LOCUS       DAVBCW010000001        63822 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVBCW010000001 DAVBCW010000000
VERSION     DAVBCW010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 63822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63822
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.001"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            212..3982
                     /gene="spuA"
                     /locus_tag="VQ514_000001"
     CDS             212..3982
                     /gene="spuA"
                     /locus_tag="VQ514_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HET2498832.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPATEPT
                     TSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDDVEKPSENWPNGALSF
                     KDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVEKLVPKMNEAWLDQDY
                     KVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQWPDGTDFTATGKYGRY
                     IDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQIFLKDDDESIYTNPY
                     YVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHLGNKVTITDVAIDEAG
                     KKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGKLGADLKEEGKQVDLT
                     LWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNKLGITDFTGYYYQY
                     QIERQGKTVLALDPYAKSLAAWNSDDAKIDDAHKVAKAAFVDPAKLGPQDLTYGKIHN
                     FKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDLGVTHIQLLPVL
                     SYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSNPKNPEKRIAEFKNLI
                     NEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPRTSFGGGRLGTTHHMT
                     KRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNPNLIMLGEGWRTYA
                     GDENMPTKAADQDWIKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKN
                     LIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEIHRRLRLGNL
                     MVLTAQGTPFIHSGQEYGRTKQFRDPAYRTPVAEDKVPNKSHLLRDKDGNPFDYPYFI
                     HDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDR
                     VHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEV
                     LADENQAGPVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTSHESTAEEKPDSTPSK
                     PEHQDPAPEARPDSTKPDAKVADAENKPSQATADSQAEQPAQEAQASSVKEAVRNESV
                     ENSSKENIPATPDKQAELPNTGSKNENKLLFAGISLLALLGLGFLLKNKKEN"
     gene            complement(4082..5131)
                     /locus_tag="VQ514_000002"
     CDS             complement(4082..5131)
                     /locus_tag="VQ514_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET2498833.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRAEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(5250..7058)
                     /gene="glmS"
                     /locus_tag="VQ514_000003"
     CDS             complement(5250..7058)
                     /gene="glmS"
                     /locus_tag="VQ514_000003"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HET2498834.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGTDNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEDGLSVLEAFKKALHIIRGSYAFAL
                     IDSENPDVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNSRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDDAGQ
                     VVVDPAIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLIHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            complement(7250..8629)
                     /gene="bglA"
                     /locus_tag="VQ514_000004"
     CDS             complement(7250..8629)
                     /gene="bglA"
                     /locus_tag="VQ514_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HET2498835.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWDRDIELLLETGHTAFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPDSPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            complement(8728..10581)
                     /locus_tag="VQ514_000005"
     CDS             complement(8728..10581)
                     /locus_tag="VQ514_000005"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET2498836.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALIEKLEASLMGDGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            complement(10594..11853)
                     /gene="rseP"
                     /locus_tag="VQ514_000006"
     CDS             complement(10594..11853)
                     /gene="rseP"
                     /locus_tag="VQ514_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HET2498837.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            complement(11875..12678)
                     /locus_tag="VQ514_000007"
     CDS             complement(11875..12678)
                     /locus_tag="VQ514_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HET2498838.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            complement(12687..13445)
                     /locus_tag="VQ514_000008"
     CDS             complement(12687..13445)
                     /locus_tag="VQ514_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HET2498839.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            complement(13676..14248)
                     /locus_tag="VQ514_000009"
     CDS             complement(13676..14248)
                     /locus_tag="VQ514_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET2498840.1"
                     /translation="MNTVKNKQEILEAFRENPDMMAILTIIRDLGLKDSWLAAGSVRN
                     FIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLEKKLREDFPQYQWELKNQVYMHQ
                     HSPHTASYTSSRDAMSKYPERCTTVGLRLNEESDFELYAPYGLEDILNFQVRPTPHFL
                     ENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            complement(14229..15200)
                     /gene="ruvB"
                     /locus_tag="VQ514_000010"
     CDS             complement(14229..15200)
                     /gene="ruvB"
                     /locus_tag="VQ514_000010"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HET2498841.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            15361..15453
                     /locus_tag="VQ514_000011"
     CDS             15361..15453
                     /locus_tag="VQ514_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HET2498842.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
     gene            complement(16090..16506)
                     /locus_tag="VQ514_000012"
     CDS             complement(16090..16506)
                     /locus_tag="VQ514_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2498843.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEQALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVLVQDLIVLPIYQRQGIGSALMKEALEDYKDAYQVQ
                     LVTEETERTLGFYRSMGFEILSTYNCIGMTWMNRKK"
     gene            complement(16518..17213)
                     /locus_tag="VQ514_000013"
     CDS             complement(16518..17213)
                     /locus_tag="VQ514_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2498844.1"
                     /translation="MPVNEYGQMIGESMEGYTPGELPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPQEMWTYLFQEPVADMGELVSLLHQMLARKDRFYYAIIDKATGKALG
                     SFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYVFEELNYRRYEWKCDAL
                     NLPSRRAAERLSFIYEGTFRQAVVYKGRTRDTDWLSMIDKDWPQVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            complement(17407..19908)
                     /locus_tag="VQ514_000014"
     CDS             complement(17407..19908)
                     /locus_tag="VQ514_000014"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HET2498845.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSTEQ
                     AEAVADYKHQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEIPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVAWLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLIRSKEIVAENNGALD
                     KVYNETVKAVTEQIDSLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAATGESISYVTWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVISVPNKLVNIVVK"
     gene            complement(20363..21451)
                     /locus_tag="VQ514_000015"
     CDS             complement(20363..21451)
                     /locus_tag="VQ514_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HET2498846.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNYPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            complement(21469..22137)
                     /locus_tag="VQ514_000016"
     CDS             complement(21469..22137)
                     /locus_tag="VQ514_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HET2498847.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHITATAIYTVIQGLLAIEVGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVTQN
                     SRSI"
     gene            complement(22156..24594)
                     /locus_tag="VQ514_000017"
     CDS             complement(22156..24594)
                     /locus_tag="VQ514_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HET2498848.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMTIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            complement(24745..26043)
                     /locus_tag="VQ514_000018"
     CDS             complement(24745..26043)
                     /locus_tag="VQ514_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2498849.1"
                     /translation="MSKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASANLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQTLGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVITQLVILAMSTLVYFPFFKVQDRLAYQNEIKQS"
     gene            complement(26060..26368)
                     /locus_tag="VQ514_000019"
     CDS             complement(26060..26368)
                     /locus_tag="VQ514_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2498850.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQASGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            complement(26414..26734)
                     /locus_tag="VQ514_000020"
     CDS             complement(26414..26734)
                     /locus_tag="VQ514_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2498851.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            complement(26929..27909)
                     /locus_tag="VQ514_000021"
     CDS             complement(26929..27909)
                     /locus_tag="VQ514_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HET2498852.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNVGIVKIEIENFDTNMFKLENYVKEKYSLESLEIIPNEFDDTPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
     gene            complement(27922..28668)
                     /locus_tag="VQ514_000022"
     CDS             complement(27922..28668)
                     /locus_tag="VQ514_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET2498853.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYETKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRVIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     CQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAVSDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            28790..29566
                     /locus_tag="VQ514_000023"
     CDS             28790..29566
                     /locus_tag="VQ514_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HET2498854.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     QMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGVNALHPEDLIDYQKVFLNHHINCYF"
     gene            29641..29838
                     /locus_tag="VQ514_000024"
     CDS             29641..29838
                     /locus_tag="VQ514_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET2498855.1"
                     /translation="MTLNEYILQYRLKQAIDKMAESPNSSLSDISDQVGFSDYKYFAK
                     VFKKYLHISPKKLKSLGRIVK"
     gene            30055..30651
                     /locus_tag="VQ514_000025"
                     /pseudo
     CDS             30055..30651
                     /locus_tag="VQ514_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466308.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            30727..31176
                     /locus_tag="VQ514_000026"
     CDS             30727..31176
                     /locus_tag="VQ514_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2498856.1"
                     /translation="MCTILVSIYYKIGLSSSGVYKAVADGEMTVGLSYKDPAVKLLND
                     GANIKVVYPKEGTVFLPASAAIVKKSKNMENAKKFIDFIISQEVQDTLGTTTTNRPVR
                     KNAKTSENMKPIDKIKTLTEDYDYVIKNKSDIVKKYNEVFTDIQSKQ"
     gene            31189..32280
                     /locus_tag="VQ514_000027"
     CDS             31189..32280
                     /locus_tag="VQ514_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2498857.1"
                     /translation="MSEIKIINAKKIYHDVPVIENLNITIPKESLFTLLGPSGCGKTT
                     LLRMIAGFNSIEGGEFYFDDTKINNMEPSKRNIGMVFQNYAIFPHLTVRDNVAFGLMQ
                     KKVPKEELIQQTNKYLELMQIAQYADRKPDKLSGGQQQRVTLACALAVNPSVLLMDEP
                     LSNLEAKLRLDIRQAIREIQHEVGITTVYVTHDQEEAMAISDQIAVMKDGVIQQIGRP
                     KELYHKPANEFVATFIGRTNIIPANLEKRSDGAYIVFSDGYALRMLALDQVEEQAIHV
                     SIRPEEFIKDESGDIEGTISDSVYLGLNTEYFIETGFASKIQVSEESTFEEELQKGNR
                     IRLRINTQKLNIFSADGSQNLIKGVNHGT"
     gene            32270..33922
                     /locus_tag="VQ514_000028"
                     /pseudo
     CDS             32270..33922
                     /locus_tag="VQ514_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(34025..34717)
                     /locus_tag="VQ514_000029"
     CDS             complement(34025..34717)
                     /locus_tag="VQ514_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET2498858.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEIIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
     gene            complement(34961..36298)
                     /locus_tag="VQ514_000030"
     CDS             complement(34961..36298)
                     /locus_tag="VQ514_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HET2498859.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPDINCAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGREGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
     gene            complement(36308..36574)
                     /locus_tag="VQ514_000031"
     CDS             complement(36308..36574)
                     /locus_tag="VQ514_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HET2498860.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            complement(36839..37225)
                     /gene="rplQ"
                     /locus_tag="VQ514_000032"
     CDS             complement(36839..37225)
                     /gene="rplQ"
                     /locus_tag="VQ514_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HET2498861.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            complement(37237..38172)
                     /locus_tag="VQ514_000033"
     CDS             complement(37237..38172)
                     /locus_tag="VQ514_000033"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HET2498862.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            complement(38215..38598)
                     /gene="rpsK"
                     /locus_tag="VQ514_000034"
     CDS             complement(38215..38598)
                     /gene="rpsK"
                     /locus_tag="VQ514_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HET2498863.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(38616..38981)
                     /gene="rpsM"
                     /locus_tag="VQ514_000035"
     CDS             complement(38616..38981)
                     /gene="rpsM"
                     /locus_tag="VQ514_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HET2498864.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(38999..39115)
                     /gene="rpmJ"
                     /locus_tag="VQ514_000036"
     CDS             complement(38999..39115)
                     /gene="rpmJ"
                     /locus_tag="VQ514_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HET2498865.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            complement(39140..39358)
                     /gene="infA"
                     /locus_tag="VQ514_000037"
     CDS             complement(39140..39358)
                     /gene="infA"
                     /locus_tag="VQ514_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HET2498866.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            complement(39475..40113)
                     /locus_tag="VQ514_000038"
     CDS             complement(39475..40113)
                     /locus_tag="VQ514_000038"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HET2498867.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGIINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            complement(40264..41574)
                     /gene="secY"
                     /locus_tag="VQ514_000039"
     CDS             complement(40264..41574)
                     /gene="secY"
                     /locus_tag="VQ514_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HET2498868.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            complement(41587..42027)
                     /gene="rplO"
                     /locus_tag="VQ514_000040"
     CDS             complement(41587..42027)
                     /gene="rplO"
                     /locus_tag="VQ514_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HET2498869.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            complement(42172..42396)
                     /gene="rpmD"
                     /locus_tag="VQ514_000041"
     CDS             complement(42172..42396)
                     /gene="rpmD"
                     /locus_tag="VQ514_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HET2498870.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            complement(42368..42862)
                     /gene="rpsE"
                     /locus_tag="VQ514_000042"
     CDS             complement(42368..42862)
                     /gene="rpsE"
                     /locus_tag="VQ514_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HET2498871.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            complement(42880..43236)
                     /gene="rplR"
                     /locus_tag="VQ514_000043"
     CDS             complement(42880..43236)
                     /gene="rplR"
                     /locus_tag="VQ514_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HET2498872.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            complement(43320..43856)
                     /gene="rplF"
                     /locus_tag="VQ514_000044"
     CDS             complement(43320..43856)
                     /gene="rplF"
                     /locus_tag="VQ514_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HET2498873.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEITLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            complement(44048..44446)
                     /gene="rpsH"
                     /locus_tag="VQ514_000045"
     CDS             complement(44048..44446)
                     /gene="rpsH"
                     /locus_tag="VQ514_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HET2498874.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            complement(44731..45000)
                     /gene="rpsN"
                     /locus_tag="VQ514_000046"
     CDS             complement(44731..45000)
                     /gene="rpsN"
                     /locus_tag="VQ514_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HET2498875.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            complement(45018..45560)
                     /gene="rplE"
                     /locus_tag="VQ514_000047"
     CDS             complement(45018..45560)
                     /gene="rplE"
                     /locus_tag="VQ514_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HET2498876.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            complement(45584..45889)
                     /gene="rplX"
                     /locus_tag="VQ514_000048"
     CDS             complement(45584..45889)
                     /gene="rplX"
                     /locus_tag="VQ514_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HET2498877.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            complement(45967..46335)
                     /gene="rplN"
                     /locus_tag="VQ514_000049"
     CDS             complement(45967..46335)
                     /gene="rplN"
                     /locus_tag="VQ514_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HET2498878.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            complement(46361..46621)
                     /gene="rpsQ"
                     /locus_tag="VQ514_000050"
     CDS             complement(46361..46621)
                     /gene="rpsQ"
                     /locus_tag="VQ514_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HET2498879.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            complement(46646..46852)
                     /locus_tag="VQ514_000051"
     CDS             complement(46646..46852)
                     /locus_tag="VQ514_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HET2498880.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            complement(46862..47275)
                     /gene="rplP"
                     /locus_tag="VQ514_000052"
     CDS             complement(46862..47275)
                     /gene="rplP"
                     /locus_tag="VQ514_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HET2498881.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            complement(47279..47932)
                     /gene="rpsC"
                     /locus_tag="VQ514_000053"
     CDS             complement(47279..47932)
                     /gene="rpsC"
                     /locus_tag="VQ514_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HET2498882.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(47945..48289)
                     /gene="rplV"
                     /locus_tag="VQ514_000054"
     CDS             complement(47945..48289)
                     /gene="rplV"
                     /locus_tag="VQ514_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HET2498883.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            complement(48301..48582)
                     /gene="rpsS"
                     /locus_tag="VQ514_000055"
     CDS             complement(48301..48582)
                     /gene="rpsS"
                     /locus_tag="VQ514_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HET2498884.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            complement(48686..49519)
                     /gene="rplB"
                     /locus_tag="VQ514_000056"
     CDS             complement(48686..49519)
                     /gene="rplB"
                     /locus_tag="VQ514_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HET2498885.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            complement(49537..49833)
                     /locus_tag="VQ514_000057"
     CDS             complement(49537..49833)
                     /locus_tag="VQ514_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HET2498886.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(49833..50456)
                     /gene="rplD"
                     /locus_tag="VQ514_000058"
     CDS             complement(49833..50456)
                     /gene="rplD"
                     /locus_tag="VQ514_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HET2498887.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            complement(50481..51107)
                     /gene="rplC"
                     /locus_tag="VQ514_000059"
     CDS             complement(50481..51107)
                     /gene="rplC"
                     /locus_tag="VQ514_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HET2498888.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RVGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            complement(51323..51631)
                     /gene="rpsJ"
                     /locus_tag="VQ514_000060"
     CDS             complement(51323..51631)
                     /gene="rpsJ"
                     /locus_tag="VQ514_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HET2498889.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(51897..52514)
                     /locus_tag="VQ514_000061"
     CDS             complement(51897..52514)
                     /locus_tag="VQ514_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HET2498890.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRYLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            complement(52511..53101)
                     /gene="nrdG"
                     /locus_tag="VQ514_000062"
     CDS             complement(52511..53101)
                     /gene="nrdG"
                     /locus_tag="VQ514_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HET2498891.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVAGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLELLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKEIYEQVKRE"
     gene            complement(53094..53600)
                     /locus_tag="VQ514_000063"
     CDS             complement(53094..53600)
                     /locus_tag="VQ514_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2498892.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKSQSAHDGGFWDVEDFSYEEWL
                     EGNRNKEMGIWLPENRVPSIQFVLFGESGCALGFLNLRLRLNEGLLNYAGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDARNGVER
                     YWIEVANE"
     gene            complement(53644..53784)
                     /locus_tag="VQ514_000064"
     CDS             complement(53644..53784)
                     /locus_tag="VQ514_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2498893.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            complement(53796..56003)
                     /gene="nrdD"
                     /locus_tag="VQ514_000065"
     CDS             complement(53796..56003)
                     /gene="nrdD"
                     /locus_tag="VQ514_000065"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HET2498894.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNNLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNCRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            complement(56123..57661)
                     /locus_tag="VQ514_000066"
     CDS             complement(56123..57661)
                     /locus_tag="VQ514_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HET2498895.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            complement(57698..57841)
                     /locus_tag="VQ514_000067"
     CDS             complement(57698..57841)
                     /locus_tag="VQ514_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2498896.1"
                     /translation="MLTKEEVNDMIEFKQTHLHRFREIETFVKLCKKSLKQPSQADNP
                     RTF"
     gene            complement(57919..59451)
                     /gene="cls"
                     /locus_tag="VQ514_000068"
     CDS             complement(57919..59451)
                     /gene="cls"
                     /locus_tag="VQ514_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HET2498897.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            complement(59597..60154)
                     /locus_tag="VQ514_000069"
     CDS             complement(59597..60154)
                     /locus_tag="VQ514_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HET2498898.1"
                     /translation="MTPLFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQQVQQPAQQPAQQNTNTANAGGNQNQAAPVQNQPVAQP
                     TDIDGTYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKI
                     YTVNGNQIVVDDMDRDPSDQIVLTK"
     gene            complement(60151..61401)
                     /locus_tag="VQ514_000070"
     CDS             complement(60151..61401)
                     /locus_tag="VQ514_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HET2498899.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRIQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLADQVKE
                     MEKTLLQTPDQLSFFEMLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVNLYQA
                     GQDFLMLNGDFSSSLLNFSQLNIGLEGVYQQENAALALQTFLLFMGEGKEAVDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGALKRKD
                     YQGMLGYLTEKLPQVELKVTGFDYQGALDERDVTGYDIVSSCREFISSFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            complement(61609..61914)
                     /locus_tag="VQ514_000071"
     CDS             complement(61609..61914)
                     /locus_tag="VQ514_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HET2498900.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            complement(61930..62349)
                     /gene="ruvX"
                     /locus_tag="VQ514_000072"
     CDS             complement(61930..62349)
                     /gene="ruvX"
                     /locus_tag="VQ514_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HET2498901.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPLDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            complement(62353..62619)
                     /locus_tag="VQ514_000073"
     CDS             complement(62353..62619)
                     /locus_tag="VQ514_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HET2498902.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            complement(62706..63275)
                     /locus_tag="VQ514_000074"
     CDS             complement(62706..63275)
                     /locus_tag="VQ514_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET2498903.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLTIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            complement(63341..63739)
                     /gene="spx"
                     /locus_tag="VQ514_000075"
     CDS             complement(63341..63739)
                     /gene="spx"
                     /locus_tag="VQ514_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HET2498904.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
BASE COUNT        18889 a        14187 c        11398 g        19348 t
ORIGIN      
        1 ttctgaataa aaatagctgg gaacacatca tcttcctaat aatacggctt ggcttatgtg
       61 acatttttat ctccgtcatt tatactgata tataaaatag tacagaaaac ttctcaaaag
      121 taggaatttt ttcacaaatc aaagaaaacg cttgcatatt gttttactac atgctatact
      181 gatatagaaa tttattgatt ggagaatcat tatgagaaaa acaccatctc acactgagaa
      241 aaaaatggtc tacagcatcc gttccctcaa aaatggaact ggttctgtcc ttatcggcgc
      301 aagccttgtc ttgcttgcca tggctacacc aactatctca tccgacgaaa gtacaccaac
      361 cactaacgaa cccaacaaca gaaatacaac cacccttgcc caacctctta ctgatacagc
      421 agctggctct ggtaagaacg aaagtgatat ttcttcacct agaaatgcaa acgcttccct
      481 agagaaaaca gaagaaaaac ctgcaacaga gccaactact tctactagtc cagtaacaac
      541 tgaaactaag gcagaagagc ccatcgaaga taactacttc cgtatccatg tcaaaaaact
      601 tcctgaagaa aacaaggatg ctcaaggact atggacttgg gacgatgttg aaaaaccatc
      661 tgaaaactgg ccaaacggag ctttgtcctt caaggatgcc aagaaagatg actacggcta
      721 ttacctagat gtcaaattaa agggagaaca agccaagaaa attagcttcc tcatcaacaa
      781 tacagctgga aaaaatctaa ccggcgataa atctgtagaa aaactagttc caaaaatgaa
      841 cgaggcttgg ttagaccaag attacaaggt tttctcttac gagccacagc ctgcaggaac
      901 tgttcgcgtc aactactacc gcacagatgg caactatgac aagaaatctc tctggtactg
      961 gggagatgta aaaaatccaa gtagcgctca atggcctgac ggaacagact ttacggctac
     1021 aggcaaatat ggccgctata tcgacattcc tcttaatgaa gccgcaagag aatttggatt
     1081 tttattacta gatgagagca aacaaggaga cgacgtgaaa atccgtaaag aaaattataa
     1141 gttcacagat ttgaaaaatc atagccaaat tttcctaaaa gacgatgatg aatcgattta
     1201 cacaaatcca tactatgtcc atgatatccg tatgacagga gcccaacacg taggcacttc
     1261 tagcattgaa agtagctttt caacacttgt cggtgctaaa aaagaagata tcctcaaaca
     1321 ctccaacatc actaatcacc taggaaacaa ggtaactatt accgatgttg caatcgatga
     1381 agctggtaag aaagtgacct acagcggaga tttctctgac acaaaacatc cttatactgt
     1441 tagctacaat tccgaccaat tcactaccaa aacaagctgg cgcctgaaag atgagacata
     1501 cagctatgat ggcaaactgg gagctgacct aaaagaagaa ggaaaacaag ttgatttgac
     1561 cctttggtca ccaagtgctg ataaggtttc tgttgttgtc tacgacaaga atgaccctga
     1621 caaagtagtt ggaactgtcg ctctcgaaaa aggggaaaga ggaacttgga aacaaactct
     1681 agacagcaca aacaaactcg gaatcacaga tttcactggc tactattatc aataccaaat
     1741 cgagcgtcaa ggtaaaactg ttcttgcact cgatccttac gctaaatctc ttgctgcttg
     1801 gaatagcgac gatgccaaga ttgacgatgc ccataaagtg gctaaagccg cctttgtaga
     1861 tccagctaaa ctcggacctc aagacttgac ttatggtaag attcacaatt tcaagactcg
     1921 tgaagacgcc gttatctacg aagctcatgt gcgtgacttc acttcagatc ctgccattgc
     1981 aaaagacttg accaaaccat ttgggacttt tgaagccttc attgaaaaac tagactatct
     2041 caaagacttg ggtgtaaccc atatccagct ccttccagtc ttgtcttact actttgtcaa
     2101 tgaattgaaa aaccatgaac gcttgtctga ctatgcttca agcaacagca actacaactg
     2161 gggatatgac cctcaaaact acttctcctt gactggtatg tactcaagca atcctaagaa
     2221 tccagaaaaa cgaatcgcag aatttaaaaa cctcatcaac gaaatccaca aacgtggtat
     2281 gggagctatc ctagatgtcg tttataacca cacagccaaa gtcgatatct ttgaagacct
     2341 agagccaaac tactaccact ttatggatgc cgatggcaca cctcgaacta gctttggtgg
     2401 tggacgcttg gggacaaccc accatatgac caaacgcctc ctagttgact ctatcaaata
     2461 cctagttgat acctacaaag tggatggatt ccgtttcgat atgatgggag accatgacgc
     2521 cgcttctatc gaagaagctt acaaggctgc acgcgccctc aatccaaatc taatcatgtt
     2581 aggcgaaggt tggagaacct atgccggtga tgaaaacatg cctactaaag ctgctgacca
     2641 agattggata aaacataccg atactgtcgc tgtcttttca gatgacatcc gtaacaacct
     2701 caaatctggt tatccaaacg aaggtcaacc tgcctttatc acaggtggca agcgtgatgt
     2761 taacactatc tttaaaaatc tcattgctca gccaactaac tttgaagctg acagccctgg
     2821 agatgtcatc caatacatcg cagcccatga taacttgacc ctctttgaca tcattgccca
     2881 gtctatcaaa aaagacccaa gcaaggctga gaactatgct gaaatccacc gtcgtttgag
     2941 acttggaaat ctcatggtct tgacagctca aggaactccg tttatccact ctggccagga
     3001 atatggacgt accaaacaat tccgtgaccc agcctacaga actcctgtag cagaggataa
     3061 ggttccaaac aaatctcact tgttgcgtga taaggacggc aacccatttg actatcctta
     3121 cttcatccat gactcttacg attctagtga tgcagtcaac aagtttgact ggactaaggc
     3181 tacagatggt aaagcttatc ctgaaaatgt caagagccgt gactatatga aaggtttgat
     3241 tgcccttcgt caatctacag atgccttccg acttaagagt ctccaagata tcaaagaccg
     3301 tgtccacctc atcactgtcc caggccaaaa tggtgtggaa aaagaggatg tagtgattgg
     3361 ctaccaaatc actgctccaa acggcgatat ctacgcagtc tttgtcaatg cggatgaaaa
     3421 agctcgcgaa tttaatttgg gaactgcctt tgcacatcta agaaatgcgg aagttttggc
     3481 agatgaaaac caagcaggac cagtcggaat tgccaacccg aaaggacttg aatggactga
     3541 aaaaggcttg aaattgaatg cccttacagc tactgttctt cgagtctctc aaaatggaac
     3601 tagccatgag tcaactgcag aagagaaacc agactcaacc ccttccaagc ctgaacatca
     3661 agacccagct ccagaagcta gacctgattc tactaaacca gatgccaaag tagctgatgc
     3721 ggaaaataaa cctagccaag ctacagctga ttcacaagct gaacaaccag cacaagaagc
     3781 acaagcatca tctgtaaaag aagcggttcg aaacgaatcg gtagaaaact ctagcaagga
     3841 aaatatacct gcaaccccag ataaacaagc tgaacttcca aatacaggaa gcaaaaacga
     3901 aaacaaactc ctatttgcag gaatcagcct ccttgcgctc cttggtctcg gtttcttact
     3961 aaaaaataaa aaagagaact aaactagccc tcctatagaa aaatccccca agcattatag
     4021 ctcgggggat taatttttgt acaatatttg ttgtcctaat aaacttgatt aggatttttt
     4081 attaagcctc tttcatagca aaataagctc gtactttggg tgcaacttgt gttccgaaga
     4141 gttcaatagc tctcagaacc tggtcatgag gcatagaacc aagcggtaga tgaagcatga
     4201 agcggtccaa tcctaaatcc tctatcatgc gaatcaattt ttcggccacc tgatctggat
     4261 tgccaacaaa catggcgcca tttggcccta cctgctccaa atattgctca taacgcaatt
     4321 cctgccagtg cggacggtct ttggaaatag catccaccac ttgcttagtc ggatggaaat
     4381 aatctttcac cgcctgctca ccatcttccg caatccaccc ccaagaatgg gctcccactt
     4441 tcaagtcttt gtcagcatgg cccgcttcgc ttccaatctc acgataagcc tgaatcaact
     4501 ttttaaaata acgtggatta ccaccaataa tagcatatac aatcggtaga ccagcctgag
     4561 caatcttcac tgttgattcg acatgaccac ctgtagctat ccacaagggc aatttgtcct
     4621 gaactggacg aggataaact tctttaccag caatcgtttg agtcaatcga ccttgccagt
     4681 ctaacttggt cttttcattg actaactgaa gcaagtctaa tttctcatca aaaagagagt
     4741 cgtagtcttt caagtcataa ccaaacagag ggaaagattc cgtgaaagat ccccttccag
     4801 ccataatctc cgctcgtcca tttgacaaag catcgatagt ggcatactgt tggaacaaac
     4861 gaatcgggtc catgcttgac agaatgctga ctgcactggt caaacggatt ttcttggtat
     4921 tgactgcccc agcggccagg acaatctctg gggctgatac tgcaaaatcc gcccgatggt
     4981 gctcgccaat cccatatacg tccaaaccaa ccttgtcagc cagctcaatc tctgccacca
     5041 actggcgaat gcgttcagca tgactgtaag tttgtccagt cccttcaagc tccgttattt
     5101 ccccaaatgt tgaaattccc aattctacca ttgtgattct ccttgtctat ctctgtcctt
     5161 caatttgaaa aattattcta acacgaatct tgagtacaag caaccaattt gctcacaaga
     5221 aaaagcctag ataactagac ttttttagct tattctaccg ttactgactt ggcaaggtta
     5281 cgtggtttgt ccacatcgag gccacggtgg agggttgcaa agtaagcgac taattgcgtt
     5341 ggtacgacca ttgaaattgg tgagaggtat ggatgtacgg tcgtaaggac gatatcgtcg
     5401 gtatctttgg ctacattctc ttctgcgata gtgaggactt tggcaccacg ggctgcgacc
     5461 tcttggatat ttccacgagt atgattggca agaactggat ctgacaagag agccaaaaca
     5521 ggcgttcctt cttcaatcaa ggcaatggtt ccgtgcttga gttctcctgc agcaaagcct
     5581 tcacactgga tataagaaat ctctttgagt ttgagacttg cttccatggc tacgtagtaa
     5641 tcttgaccac gtccgatgta aaaggcgtta cgagttgttt caagaagttc acgaaccttg
     5701 gcttcaatgg tttctttctc tgaaagagtt gattcgatag actgagctac gattgacaat
     5761 tcatgaatca ggtcaaaggc ttgcgcttta gcattaccat ttgcttctcc gactgctttt
     5821 gcaaggaagg caagggctgc gatttgcgct gtataggctt tagttgatgc cacggcaatt
     5881 tcaggacctg cgtgaaggag catggtatag ttggcttcac gtgagagggt tgagcctgga
     5941 acatttgtca ctgttaagct tggaattccc atttcattag ccttgaccaa aacttgacga
     6001 ctatccgctg tttcaccaga ttggctgata aagatgaaga gtggtttctt gctgagaagt
     6061 ggcataccgt agccccactc agatgagatg ccaagttcaa ctggtgtatc tgtcaattct
     6121 tccaacattt tcttagaagc aaatcctgca tggtaagatg ttccagctgc aaggatgtag
     6181 atgcggtctg cgtcttgaac agccttaatg atagcaggat caaccactac ttgaccagca
     6241 tcatccgtgt aggcttgaat gagtttacgc ataacagttg gttgctcatc aatttcctta
     6301 agcatgtagt aaggataagt tcccttaccg atatctgaca agtcaagttc cgcagtatag
     6361 ctagcacgtt cacgactgtt accatcatag tcttgaactt ccacgctatc agccttgacg
     6421 attaccaact cttggtcatg aatttccatg tattggttgg tttcacgaat catagccata
     6481 gcatctgagc agaccatatt gtagccttcc ccaagaccaa tcaaaagtgg agatttgttt
     6541 ttcgctacat agatgacatc tggattttca gagtcaatca aggcaaaggc atatgaacca
     6601 cggataatat gaagagcttt tttaaaggct tcaagaactg agagaccgtc ttcttccgca
     6661 aattttccaa tcaaatgtac ggcgatttcc gtatctgttt gccctttgaa gtggtgccca
     6721 gcaaggtatt cttctttaat ttcaaggtag ttttcaatca ccccattgtg aaccaagaca
     6781 aaacgttctg tctcagagcg gtgtgggtga gcattgtctt ccgttggttt cccatgagtt
     6841 gcccaacgag tatgtccgat accagttgtt ccctcaacac cagctgtctt agcagacaat
     6901 tctgcgatac gaccaacagc cttcaccaaa tggttatcag taccatctag gacaaaaatt
     6961 cccgcagaat catagccacg gtattcaagc ttttcaagcc cttgaatcaa aatatcagtt
     7021 gcatttgtgt ttccaacaac accaacaatt ccacacatag tatatacgac acaggcaagc
     7081 tgtgctttct ccttaaaatt ggtatagtct aattcatctt ttatagaatc agcaaaaata
     7141 gtatatactt gtttctttca cttgtcaaga gtaaaaattg gtatagttca aattaagctc
     7201 ctgtaagcaa aaaactctga ccgattggga taatcagtca gagtcctttt taaaatccat
     7261 tattatcgct taattctttg aaccagtggc ctgatttctt cagacgacgt tcttgcgttt
     7321 ccaagtctaa ttcgaccaaa ccatagcgat ttttatagct gttgagccat gaccagcagt
     7381 caataaaggt ccaaatcaag tagcccttac agttggcacc atcttcaatg gcacggtgaa
     7441 gttcacgaag atgacctttt acaaagtcaa tacggtaatc atcttgaatc attccatctt
     7501 gacggaattt ttcttctcct tcaacaccca taccattctc agtcaacatc cactcaatat
     7561 tgccataatt ttccttgata ttttgggcga tgtcataaat tccttgctca taaatctccc
     7621 aaccacggtg agaattgatt ttacgtccag gcatcacata aggctcgtaa aaatgttctg
     7681 gtaagagtgg actatctgga tgcttagcaa atcgaggagc cataacacgc aaaggttgat
     7741 agtagttcac accaaggaag tccaccgtat tatcacgaat gagttccaac tcttcctctg
     7801 tagcatcagg taaaagaccg tgttcatgca agatttctac caactcctgt ggataagtcc
     7861 ccaagacaga tggatctaag aaagattggg cctgaaaaag ggccgcaata cgagctgcct
     7921 tgacatcagc aggatgctgg ctacgtggat aagccggtgt caagttgagg acaatcccaa
     7981 tcttggaatc aggcaaaagt tcatggcaag ccttaacagc ccggctgctg gccaattgtg
     8041 tatgataggc taccttaaca gctgcctctg catccacctt atgtggataa tgggcatcat
     8101 aaaaataacc aaattctaca ggaacgatgg gctcgttaaa ggtaatccat tgatccacta
     8161 aatctccata agtctcaaaa caaaaacgag catagtcttc ataggctgag actgtcgcct
     8221 tattttccca accatcacca tcctcttgaa gggcaaaagg taaatcaaaa tgatagagat
     8281 tgactaacag acgaattcct ttagccttaa tagcctcaaa gaccttacga taaaaatcca
     8341 caccttgagg gttgactttt ccacagcctt gtggaaaaat ccgtgaccac tgaatagaag
     8401 tccgaaaggc tgtgtgacca gtctctaaca aaagctcaat atcccgatcc caattttcat
     8461 aaaaagtcga tgtcttatct gaaccaatcc cattatagta acgatttggc tccacttgga
     8521 accagtaatc ccagagattg tctcccttgc cgtcaccagc tacacgtcct tctgtctgcg
     8581 gtccagaagt agaggatccc cagacaaaat cctttggaaa tcttagcata catttacctc
     8641 tttatctact catttctccc attatacaga aaaaacaagg taaaaactag ttacattttt
     8701 tccttgtttt tcttctgatt atagttttta tttcttgctt aggatttcaa gcgtttcaag
     8761 cacgttatct gcatgaacct caatggtgtc accagttgcc ttgatcttaa cttctacaat
     8821 gccatcggcc gcttttttac caacagtgat acggattgga agaccaatca agtcactatc
     8881 gctaaattta acaccgacac gttcgttacg gtcatctgtc aagacttcat aaccatctcc
     8941 catcaagctt gcttcaagtt tttctatcaa ggcttgcgct tcttcatcct tgacattgac
     9001 agtaatcaaa tgcacatcaa atggtgccaa ttctttaggg aaattgattc cccaagcgta
     9061 acggtattca ccttttggcg ttttgttaac aaagaggcga gcgtgttgct ccatcactgc
     9121 tgaaagaaga cggctgacac cgataccgta acatcccatg atgattggca cagcacgacc
     9181 attttcatcc aagacatctg ctcccatgct tgctgagtag cgagttccga gtttgaaaat
     9241 atgaccaatc tcaataccac gcgcaaagtt aaggacacct tgtccatctg gggaaatttc
     9301 accctcacga acttcacgga tatccacata ttctgcagta aaatcacggc ctgggttcac
     9361 accagtcaag tggtagccat cttcgttagc accgacaact gcattgcgaa catcttgcac
     9421 cttacgatct gcaataattt taatattctc tggcaaacca actggtccaa gtgaaccaaa
     9481 tcctgcttga acaacattcg ccacttcttc ttcgctagca acgtcaaaga aatctgctcc
     9541 taagtggttt ttcaacttga cttcgttgag ttggtcattt ccaactagaa gggctgcaac
     9601 aagctcacca tctgcaatgt agaagagggt tttaatcgtt tgttcttctg gaacattgag
     9661 gaaagctgca acttcatcaa ttgatttaac atctggcgtt gcaacacgag taacttcttc
     9721 ttcagcgaca acacggttgc ttggtttgta ctcgtttgtt gccatttcta agttagctgc
     9781 atagctagac tcacttgagt aagcaatggt atcttcacca gagactatcc atttgagcaa
     9841 ttctgccttg atttcttctt gcacttctgc aggaatttcg tcaaatgagg caactgactt
     9901 gtccaagaca acccagcggt caaggtctgt acgagcagat gtaatggcca taaattcttg
     9961 gctatcctta ccacccatgg ctccaccgtc accaataata gccttgaagt ctaaaccact
    10021 acgagtgaaa atacgctcat aggctgcttt gtactcatca taaacactat ccaaactatc
    10081 atagttagcg tggaaactat aagcatcctt catgataaat tcacgtgtac gaagaagtcc
    10141 attacgtggg cgtttttcat cacgatactt gggctgaatt tgataaaggt tgagtggcaa
    10201 ttgcttgtaa gatttaacag aatcacggac aatagctgta aaggtttcct cgtgagttgg
    10261 acctaagata aagtctgatt tttcacggtt ttttagtttg taaaggtctt caccataggt
    10321 ttcgtaacga cctgattcac gccacaattc tgcactaaga agggctggag ccaacatctc
    10381 aacagcacca atcttttcga attcttggcg catgatgttt ttagcttttt caatcacacg
    10441 gttggcaagt ggtagataag aataaacacc tgctgaaact tggcgaacat aaccagcacg
    10501 caacataaga gcatggctga taacttgagc atcgcttggc atttcgcgaa gcgttgggat
    10561 aggcatttta ctttgtttca taatattcct cgattatcta aaaaagagtc gcataatgtc
    10621 attccaagtc acagcaatca tcaagacaac catgatgacc actccggcca aggtgacata
    10681 ggtttcaatt tcttgtttca atggtttgcg gcggatggct tctaggatat tgagcacaat
    10741 cttaccacca tccaaggctg gaatcggaat aagattaaaa atcccaatat tgatggaaat
    10801 cattgccaag aagtacaaga tattttcaat tccattttta gcagcatcac tacttgcctt
    10861 aaagatagca acaggtccac ccaacttgtt caaatccggt tggaaaatca gatttttcag
    10921 agctgagaga attcggagag ctgagtcagc agcagttgta aaaccaccta caaacatgga
    10981 tagaaaatct gacttaaccc ccggttgaac acctagaagg taacgacctt gactatcttc
    11041 gggtgtaaca gtgacttgtt tgtcactccc cttttcagaa atagtcacat ccaaagtcgg
    11101 tgccgtctta tctttggttt ctgtttccac agcttggatc aagctttccc agttgctaac
    11161 ctcatgtgag ccaatcttgg taatttgtgc cgtttctggt actcctacct tggccaaggc
    11221 accttggggc atgatatgga actgattggt atcaacatct ctgacaccac cctgcataaa
    11281 gattaaaacc caaaaaacaa cgacacctaa gataaaattg ttcatagggc ctgcaaaatt
    11341 ggtaatcagt ttgccccaga tagtcgcatt ttgatattga acatctaaag gtgcaatccg
    11401 aacctcagta ccatctgctt ccacaaccgt tgcatcgtga tccactgcaa atgttttttc
    11461 ttcttccaga accaatcctt tgataaagag cttgtcttca aaatcaaact gggtcacctg
    11521 catagggagg gctgtttgat ccaatttttt acctgagaga ttgatgcgtt taaccttacc
    11581 atcatcagca agtgtcaaac taacaggcgt tcctgtcttg atttcagttg tatcatcacc
    11641 ccaaccggcc atgcggacat agccacccag aggcaagatt cgaatggtat aggccgttcc
    11701 atccttgcca atgtgagcaa aaattttagg tcccataccg atggcaaatt cacgtactaa
    11761 aatccctgat ttcttggcaa agtagaagtg cccgaactcg tgcaccacta caataatccc
    11821 gaaaaccaga ataaaggtta aaattccgag catagcgttt cctccgtctt ttgattaaaa
    11881 gagtccaaat aagtgcatga ttggaaatac aagcaacata ctatcgaaac gatccaaaac
    11941 accaccatgt ccagggataa atttcccaga atccttaaca ccaaaatgac gtttgatcga
    12001 actttctagt aaatcaccaa attgtccagc aatgctaaag aaaatagcaa agactgacat
    12061 cttgtaaatt ccatatggaa gagcaactgt actgtcaact atcataaaga taatggttac
    12121 taaaattgct cctaaaatac cacccaaggc accctcaagg gttttattag gcgataccct
    12181 tggtgctaac tttcgtttcc catagttcat cccaacaaga taggcaccac tgtctgtcgc
    12241 ccagacgata cacaaggcta agagagcctt gtccaaacct gcaacacgag catctagtaa
    12301 agcattaaat ccaaagccca cgtagaagct catagcaaga gggaaaaccg catcctcaat
    12361 cgtataagac ttgctaaaaa cggtcgttcc taacatgatt gaaatcaaaa cactataggc
    12421 aaccacattc ccatcaactg gcaaaaaagt caggtaattc tccaagggaa tggtcaatgc
    12481 aaaggttgca aagagggtca agaggccctc catcgtcatg gtctctagac ctctcatctt
    12541 caaaagttca tgcatggcta gcatggctat gattccgatt gctatctgaa gcaagaggcc
    12601 cccaatcatt aaaattggta ggaaaatagc cagggcaatc cctgcaaaca aggttctttt
    12661 ctgtaaatcc tgggtcatat ttcctcctaa actcctccaa atcggcgatg acgacgatta
    12721 taggcaagaa tagcttcctg caaggccgct tcgtcaaaat caggccataa ggtgtccgta
    12781 aaataaagct cactataggc tccctgccat ggaaggaaat tgctcaaacg caattctcca
    12841 ctagtacgga taatcaagtc tgggtctcgt aagtccttag gcaaatgctg ggtaaagaga
    12901 tagttaccaa tcaattcctc tgtgatgtca cctggattga ttttggcatc taaaacatcc
    12961 tgggaaatca acttaagcgc ctgtgtaatc tcagcacgtc caccatagtt aagagcaaaa
    13021 ttaagaatca atcctgtgtt gttcttagtc aattcctcag ccttggttaa agcttcaaag
    13081 gtttgcttag gcaggcggtc tgtctcccca atcatttgaa tcttaacatt attcgcatgt
    13141 agttccggga cataattatc ataaaactct actggcaagt tcatgataaa cttgacttcc
    13201 tgatctggac gggtccagtt ttccgtagaa aaagcataga ccgtaataac cttgacgccc
    13261 agtttgttgg ctgccttggt cacggtttgc aatgcttcca tgcccgcctt atgtccaaaa
    13321 actcgcggtt gcatacgttt tttagcccaa cggccattgc catccatgat gatgccgata
    13381 tgagcaggaa cctgtgtcgg aacctctact tccacagcct tatctttctt aaaaaatcca
    13441 aacatgatct tattcctatt caaaaatcta tcgttttatt ataccatatt tccccatttt
    13501 cttctatcac taagctattt attctcaggc accaagccca tttttcaaaa aaataagccg
    13561 cctgattggg cgactttatt tttataggga gattattatg aaaaagtttt aggagtttaa
    13621 gttaaggtct tcttaactta tgaacttagt gtacactccc tagcttaaag tttccttaag
    13681 tatttttaaa aatcaaattt ttccatttct cctgccaatt tttcttggat aaacgtgttt
    13741 gatagagttc cattcggtct tcattttcta agaaatgagg agttggacga acttgaaaat
    13801 tcaaaatatc ctccaaacca taaggtgcat agagttcaaa atctgattct tcattcaagc
    13861 gcagtccaac tgtcgtacac cgttctggat acttactcat agcatcacga gaactggtat
    13921 aggaagcagt gtgaggactg tgctgatgca tatagacctg atttttcaat tcccactggt
    13981 actgaggaaa atcctctcgc agctttttct ccagtaataa ggtttcctca taagaaaaat
    14041 ctggatcaaa gaaaatcaca tctatatctg tttcatgatc aaaaggggat ttgtctgaca
    14101 aaagattcca aatgaaattt ctgacagaac ctgctgccaa ccacgagtct ttcagaccaa
    14161 ggtctcggat gatcgtcaga atggccatca tatctggatt ttctctaaaa gcctctaaga
    14221 tttcttgctt atttttcact gtattcataa cctaagtgct catatgcctt agcagtcgcc
    14281 acccgtccag accgtgtccg catgataaaa cctttttgaa tcaagtaagg ctcatacata
    14341 tcttcaactg tctcacgctc ttcggcgata ttcacagaaa gagttcctag accaacaggt
    14401 cctccactgt acatctcaat catggtacga aggatttttt gatccacata gtccaaacct
    14461 tcatggtcaa catccagcat agtcaaagcc ttatcggtaa taatatcatc aattacccca
    14521 ttccccatta tctgggcaaa atcgcgcacg cgcttgagga gacgattggc aatacgaggg
    14581 gtcccacgac tacgtagggc caactcagat gctgcctcat gagtgatttc catctcaaaa
    14641 atatctgccg tccgctcgac aatttctgtc aagtcagcat gggcataata ctccatatgg
    14701 cctgtaatcc caaaacgtgc ccgtagcgga ttggagagca taccagcccg agtcgtcgca
    14761 ccaatcaagg taaaaggtgg taactccaaa tgaacactac gactaccctc accagcccca
    14821 atcataatat ctatgtagaa gtcctccata gcactataaa gcacctcttc cactgacatt
    14881 ggcaaacgat ggatctcatc aataaaaagt acatccccag gctctaactc attcaaaata
    14941 gctaccagat ctccggcttt ttcaatgact ggacccgacg tctgcttaag attgactccc
    15001 agttcgttgg caataacaaa ggccatggtc gttttcccga gacctggagg cccaaataag
    15061 agcacatgat ccagcgcttc atcccgcatt ttggcagctt caataaagat ttgtagctgg
    15121 tccttgacct tatcctgtcc gatatattca cgtaaatact gaggacggag cgtgcgttct
    15181 actaactcct catcccccat tatctcatta tctaaaattc tactcatggc tctattatat
    15241 caaaaaacca agccacaaac aaaaaagcca cctgattggg tgactcctaa gtttagcact
    15301 tatgtggtat aatattctac gacactgcta caccgcctac gaaaggaggt gagatagccc
    15361 atgatggaat tagtactcaa aactatcatc ggaccaattg tggtcggtgt cgttcttcgt
    15421 atagtcgata aatggctaaa caaggacaaa tagtgtcaaa aaagacctca agcttatttg
    15481 gtcgtgagct tggggtcttt tctagcctat gatatagaac tagtactcaa ttccttttta
    15541 ttatcccata gttcacgaat tttgtcaaag gtttacattt tcttcaaccg ctgtacgaca
    15601 agacggttaa gattaagaga acgttaggga ttctatcaat ttcatagaaa ttttgatttc
    15661 gtaaacgaag agacaatctt acatgtcact tctcatttaa tacgccacta ctagacaagc
    15721 aaaatcatta ttacagtagt tccagttctt caattaacag tcacttacaa tcaaattgag
    15781 tttgaactag ctgaagcgac cacagaccta tttcttagtt atattcgcta aaaaagtccc
    15841 cgccaattcc ccgaccaaaa tccgaaaaat accgaaaaat accgaaaaat atcgaaaaat
    15901 tatttttaga atagtcccca aaagcctgaa atagagctaa aaaactccac ctgattgggt
    15961 ggagttaagg gagattatta tgaaaaagaa aagtttagga ttttattaaa taaagttagg
    16021 aggtctttat ttaatgacta tataatacta gaccatcctt aaattttgct taagaaaaaa
    16081 caagttttgt tattttttac gattcatcca agtcatccct atacaattat aggtggataa
    16141 gatttcaaag cccatagaac gatagaagcc caaggttctt tctgtctctt ctgtcaccag
    16201 ctggacttga taggcatctt tgtaatcctc taaagcctct ttcatcaagg cactaccaat
    16261 tccttgacgc tgatagattg gtaaaacgat taaatcctga accaatactg atgagaatcc
    16321 atctccaacc aaacgaatca agcccaccac agcatcacca tcaagtgcca gataaattac
    16381 taatgaatga gataaggcct gctccagcat ctctggttga tgggtataat ttgtccaacc
    16441 gacagcctga tagagatgta aaacatcctc tagcttgaca atttcttgct ttttaatagt
    16501 aatcatctca acacctctta aagttctctc aagctcttgt actgctgtcc atttttatca
    16561 aaattttcag gacgcaacca tgtttccaaa cgatctttga cttgaggcca gtccttatca
    16621 atcatagaca accaatccgt atctctcgta cgtcccttat aaaccactgc ctgacggaag
    16681 gttccttcat aaataaagct caaacgctcc gcagctcgtc tggatggcag atttaaggca
    16741 tcgcatttcc actcgtagcg acgatagttt agctcctcaa aaacatagcg agctaggaga
    16801 tactgagctt cagttcctat ccgtgtaccc cttagctctg gagaaaaagt gactgctccg
    16861 acttctatta ctcggttatt ctggtcaatg cgcatgagag aaaaagatcc caaagcctta
    16921 ccagtcgcct tgtctatgat tgcatagtaa aaacggtcct tacgagccaa catctgatgt
    16981 aaaaggctaa ccagctcccc catatctgct actggctcct gaaaaaggta ggtccacatc
    17041 tcctgaggcg tatcaggccc ataaacagct agcaaatcct ccgcatgctt ttccactgaa
    17101 agagcctcta tcctagcata acgcccttct aagaaatcaa tagaaggcag ttcacctggt
    17161 gtataacctt ccattgactc cccaatcatt tgaccatatt catttactgg catagctttc
    17221 tccttttttc acttttcaaa ctttatagtt gcccatagta taccatactt tcataagaag
    17281 attcaaaaaa tcctccagat tctactctga aggattccta ccttatttca caacgatatt
    17341 aacgagttta gttggctcaa acagtttggg aaactgtttg aggttggaaa tcagcaaata
    17401 aattggttat tttacaacaa tattaaccaa tttatttggt acactaatca ctttcacgat
    17461 ttccttaccg tcaatttctg ctttgacttt ttcatccgct agagcaattt cttgcaattc
    17521 ttcacgtgat aggtctttag cgaccatgag tttggcacgg actttacctt tgatttggac
    17581 aacgatttcg atttcgtctt caaccagttt actttcgtcc catgttggcc aagtcacgta
    17641 agagattgac tcacctgttg cagcaacagt ttgccagagt tcttctgcca agtgaggtgc
    17701 aaatggggca atcaattgga taaatccttt ggcatagtca acatagagct tgtcttcctt
    17761 gttagccgca ttgacaaaga ccataagttg ggcaatggct gtgttgaatt tgagggaatc
    17821 aatttgctct gtgacagctt taacagtttc gttgtaaacc ttgtcaagag cgccattgtt
    17881 ttccgcaaca atttccttac ttctaatcaa acggtaaaca cggtcaagga acttacgact
    17941 tccttccaga ccttcttctg accaagcaat cgaagcatcg agtggtccca tgaacatttc
    18001 atagacacga agggtatcag caccgtattg ttccaccaca tcgtctgggt tgacaacgtt
    18061 cttgagcgat ttagacatct tggctggcgc ttgctccaac tcttcccctg tttccacatg
    18121 gaagaaagaa ccatcacgtt tttcaacctt gtcagtcgcc acaagagccc cacggtggtc
    18181 acgatagctt gttcccaaaa tcattccttg gttaaagagt ttttggaatg gttccttagt
    18241 tggaacaaca ccgaggtcat agaggaattt atgccagaaa cgagcgtaaa gcaagtgaag
    18301 tacagcatgt tccgcaccac ccacgtagat atctactggc aaccattgtt tgagcagatc
    18361 ctcgtcagcc aatttctcag tattgtgtgg gtcaatatag cggaggtagt accagcttga
    18421 acctgcccat tgtggcatag tatttgtttc acgacgacct ttgacaccat cttcacgagt
    18481 cacttcaagc cagtctgtca agttagctag tggactttca ccagtacctg agggacggat
    18541 atccttggtt acaggcaaaa caagtggcaa ttcactttca gggacagctg ttgaagttcc
    18601 atcttcccaa tgaatgattg ggattggctc accccagtaa cgttgacggc taaagagcca
    18661 gtcgcggaga cggtaggtta ccttctcttg tccacagcct ttttcttcca accaagccac
    18721 aatcttggca atagcgtctt ctttgttgag tccatctagg aagtctgaat taacgtgaag
    18781 gccatcttct gtgtaggcag cttcttcaac atttccacct tcaagtactt cgacgattgg
    18841 aaggtcaaat tgtttggcaa attcccagtc acgttgatcg tgggcaggca cagccattac
    18901 cgcacctgta ccatagctag caagaacgta atccgcaatc cagattggaa tttccttacc
    18961 attgacagga ttgatggcat aagcaccagt ccatacacca gttttttctt tggcaaggtc
    19021 ggtacgagcc aagtctgact taaggctggc ttggtgtttg tagtctgcta ctgcttctgc
    19081 ttgctctgtg cttgtgatgg catctactaa ttcatgttca ggagccaaaa cagtgaaagt
    19141 cgcaccgaaa agtgtgtccg gacgagtagt aaagactgta aattccttgt ctgttccttt
    19201 tactttgaaa gttacattgg caccagttga tttaccaatc cagttgcgtt gcatatcctt
    19261 gatagactct gaccaatcta gttcatctaa gtcattgagc aagcgctctg cgtaagccgt
    19321 gattttgagc atccattggc gcattggttt gcggacaact ggatagcctc cacgttcaga
    19381 agttccgtca ggaagcactt cttcattggc aatggcagtt cccaattcct caacccagtt
    19441 tactggcact tcagcttcat aggccaagcc tttttcgtaa agcttggtga aaatccattg
    19501 agtccacttg tagtagtttg gatctgttgt gttgacttca cgatcccagt cataagaaaa
    19561 tccaagcgca ttgatttgac gtttgaagtt ggcaatgttt tccgctgtaa attctgctgg
    19621 gtcattacca gtatccatag cgtattgctc tgcaggcaaa ccaaaagcat cccaacccat
    19681 tgggtgaagg acattgtagc cttgcgcacg tttgtaacga ctgaggatat cggttgcagt
    19741 ataaccttct gggtgtccta cgtgcagacc agctccagac ggataaggga acatatcaag
    19801 cgcataaaac ttaggttttg atgcatctgt tcctgtctta aatgtatgat gttctgccca
    19861 gtagccctgc cacttaggct caatttcttt atgattgtaa aaactcatgg tctctctcca
    19921 attttgtgat gttactatta taccattttt gagggaattt gaaatgtgtt taaaggaaaa
    19981 actgtatact gtatctcatt aattttgatg actatgaatt gctctatctc ccacttttta
    20041 tcccattgtt atgcagtaaa tcaaggagta agtcaagaaa cttatctcgt atagcacaaa
    20101 atacttattt tagaccgaag ataaaactgg aatgctaatt aatgtattca caataaatag
    20161 ccgacaacct atgtcgcttt ttgatttatg aagaacgtat agtggactga atctgaaata
    20221 gtacgaaaca attgctaaaa tacttataga aattaatttt actttcccaa tcgatttgtt
    20281 ctcatcttat ttcaatctgc tatagaattc aatacgagga tcaataaaag actaccattt
    20341 ggaaaacagt agtccttatt gtttattttg aatttacata ggcatctaca gcgataatag
    20401 cttgagcaac atctgaaggc gaaatcttaa acggcatctg atgaattgtc tcaccctcca
    20461 tagttgcttg tttaccaact tttattaaat catcatatcc aacttgatcc aaatgcattt
    20521 cttttagagt tgttggcata ccaatttttt tgtaaaactc aatatactta tcaagttctt
    20581 ctttaggtct attttccaat aatagttgta ctaaagttcc ataagctact ttttcaccat
    20641 gtgttaaatg atgaatgtca cctgtcaatg cagtaaaacc attatgaatt gcatgcgccg
    20701 cagctaatcc tccactttca aaacctaggc cactcaataa agtattagct tcaacaatat
    20761 tttctaatgc tggtgtcacc actttagctt cacaagctgc catagcctgt aaaccatctg
    20821 caaacagcgt ttcttcacat ttcttcgcaa ttgcaactcc agccaatgtt tgctgttgtc
    20881 ccaacatagt ttttccattt gcctgcataa ccgcacgcgc ctcaacccaa gttgctaaac
    20941 catctgcaat accagacgct aataaacgct taggggcttg tgaaataact tttgtatcaa
    21001 ccaaaactaa atctggattt ttagaataaa atagataatg atcaaatgca ccttcatctg
    21061 tataaataac agataaagca gatacaggtg cgtcggtcga tgcaattgtt ggagcaataa
    21121 taacaggctt ttcaatcaaa tctgcaatag cttttgcgct atcaatcgtc tttcccccac
    21181 caagaccgat aatactatca caattttctt tctcagccaa ggcaacaact cgattgattt
    21241 cattgtcaga agcttcacca ttaaatagcg ccagaacaat atggaaacca tacctatgta
    21301 gataatcttc aaatcgtttt ccaacaatat cataaaccaa ctgatcgcat aatagaatag
    21361 ggtaatttcc caaatccaaa attgatttgg cattttcaaa caaggcattt tccccctgaa
    21421 tatatctaga aggactagca aaaattctca tatgtatttc ctctctatct aaatggaacg
    21481 actattttga gtaacaaacc aatcgtcaga aaaatcatca accgcctttt ggatagatgg
    21541 catggcgaaa gctgattcaa aaacatctgc tcctgctgta acagcatgcg cacctgcagc
    21601 taaagcatta tttacttgtg ctacattttt aaaggatgca gctaaaatct tactaggaga
    21661 gttctgtcta tcaatagcaa gagctaattg acgaatgaca gaatttgaat caatgttcag
    21721 attttccatt ctattataat atggagctag gtaatccgct cctacttcga tagctaataa
    21781 tccctgaata actgtataaa tagctgttgc agtgatatgg tagccctctt tttttagcac
    21841 ctttattgca cgtaatccag ctggagtaac aggtactttg ataaatatat catctcctgc
    21901 ttgtcttcga atttcatgag catcctttaa gatgccttca aaatcttgag aaatcacctg
    21961 aacatgaata gagggtgtag agccaatcaa ttctcttaca tctttgattc gttcaaaaaa
    22021 attaatagaa ccctctcttt ttgcaatagt gggatttgaa gttaccccag ctagcggcaa
    22081 aatttcagac cactttttaa tctcatctaa atttaatgtg tcaagcataa attccataaa
    22141 aagaacctct ttattttaca aagtatgctc agtacgtcct ataatgtcat cttgggttgc
    22201 cttagaaaga acattgaaga atgcagagta tcctgcaaca cgaacaatta agtctctgtg
    22261 tttttcagga tgtttctgag cgtcaatcaa cgtctctctg gaaacaacat tgtattgaat
    22321 atggtaccca tgtaaacgat taaagaatgt tcgtaacaaa gcaattagtt ttaatttatc
    22381 ttcttctttg gctaacgttt gaggatttac tttctgattt aagagaaccc cacctacgat
    22441 ttcatctgtt ggtaattttg aaacagattt taaaacagat gtagggccgt gttgatccat
    22501 attatgtgat ggtgaacaac cctctgctaa cggtgtaccc gcgttgcgtc catctggagt
    22561 tgctaatgtt ccacgtccct gccctacgtt ggctgagata gaagatgttc ctgaataacg
    22621 aattcctcca ataggccctc ttccataacg tgtattagga tatttagcaa tttcatcaac
    22681 ataaatgtca taagcagcag taaccaattt gtcagcataa tcatcatcat taccatactt
    22741 aggtgcatca tgaatcaaca tttcttgaat gaccttacct tcttctccgg cataatctgt
    22801 ttccagtgca tgccaaagct gacttgggct tatacgttct tcctcaaaca ccaatttttt
    22861 aattgcagct aatgaatccg acaaatttgc aattccaact tgcaatcctg atatataatc
    22921 atatactgct ccaccttctt taaggtgttt tccacgacca atacaatcat cagtcaatgc
    22981 tgaacataga atatcaggaa cttctcgttc caatgataaa tcaatagaat tttcaacaat
    23041 aacactcatt cgtgtcaaat atcttagtgt tttatcccaa gcattttcta attcagaaaa
    23101 gttcttcata tccttaaaat gaccaaagct tggtgcaaac cgtttacccg aagccggatc
    23161 aattccatca ttcatcgtga taagtagaac cttagggaag ttcatataac tcatacctgt
    23221 gcaacgatag ccccatttcc ctggaactgc cgtttcaaca catccaatgg cactgtaatc
    23281 ataagcatca tcttccaata ctccttttgc aataaaagaa ggaataataa tctcatcatt
    23341 attaaatgca ggcataccaa aaccaagttt catcacttca atacactcat tcatgaaacg
    23401 agcatctaaa cctgcatggt aacgtacagt tagattaggt tgcggtagat gggtttgtgc
    23461 aactgatttt aataccaaat aagataatgg gttaacagca tccttcttat ctcgagtctg
    23521 tccaccaatt gtaacatttt gatataaagg acttcctgct gaagaaaatg tatgtgattg
    23581 actgcgaacc ttattgattg taattgtctt aatccaaaga tttgtcagac gttcaacaat
    23641 gctatcttct gtttctttac cactttctaa atcagccttc atatatggat acatatattg
    23701 atcaaaacgg ccatatgaaa gagagtggcc attagattca atttgtaaaa tacattgaat
    23761 aaaccaaact gattgaatag cttctgcaaa agtagttgcc ggttcatatg ggactctaga
    23821 gcaaatatct gcaatctcaa gtaattcttt cttacgttta ggatttgcat tttcggctaa
    23881 acttttagca agagcaacaa agcgctttgc atatacttta atagcatcga ttacgataaa
    23941 tatagagtcg taaaaatgat atttatcaat acttgctgga tctgttaaat ctagagctac
    24001 ttttgcttta cgagcccgct cttcaaaacc tcttaaacca aattgcaaaa gtttctgata
    24061 gttaactgct aggtgagcat ctccagaatt catcttacct tccataccga agaatcctgt
    24121 ttccatataa acagacactt cttcaggtaa taaggcacca gctctagcac gtaaattatt
    24181 attttcccaa aacggagcaa tacttctaag ttgttctttt gtttcttctg taatatagaa
    24241 aacatctcca tcacgctttt caaaaagatc caactcattg agaacaaatt ctagcgtata
    24301 ttccggaaaa ataggagcat ctttattgga agaagcttga tttcccgcaa tcatagattc
    24361 ttcttcaata tagatagtca tattttccaa aatttctttc agcatatatg cacgttttag
    24421 gacattaggt tgttccttgt atcgttcata ggcgcgtgtt gctagaactg ctctctcagc
    24481 atcaatataa ggttttttat ttaaaacatc ttctcgatat ttgttcatcc tttcagtaag
    24541 gctgccaaaa tattctgtct tgattgttgt tcttgctact tctgtattta ccatcgtaac
    24601 cacctttctt tcgaaaatat ttttatttct ttcgaaaata taataccgat ttttatagaa
    24661 attttcaagc ttttaactag aaaaatttta actctctaat ttttagcaca aatgtgtgag
    24721 tttaacagta acacacaaat acctctaaga ttgtttgatt tcattttggt aagctaaacg
    24781 atcctgtact ttaaagaatg gaaaataaac caatgtagac atcgctaata tcaccagctg
    24841 agtaataact ccttgccatc cacccaccaa aaatcctgat aaaatagctg gtgtactcca
    24901 aggcaatgtt acccctgaga atggctgcat gaaacctgtt gcaatagctc catatactat
    24961 cacagctgca agtacaggaa caagaatgaa aggtacaaac ataactggat tcatgacaat
    25021 cggaaatcca aatacaactg gctcatttac gttaaatatt gctggaaaag ctgcaacttt
    25081 tcctaaggtt tggtattgtt ttgattttgc tgcaaaaagc atggcaacta caagaccaaa
    25141 cgtaatccct gaacctgata gaattaaaaa tgaatctaaa aattgttgag taacaatatg
    25201 tgcaccattt tctaatgata gattagcaga ggctaacata gctttattag cgtcaagatt
    25261 agataaaagc agagctgtca ctactccatt tactaccgat tgcccatgaa caccaaacca
    25321 ccacaaaaat gatataaaga atgcaattcc aatagcacca tacaaagatc cagttaaacc
    25381 ttgcaacgga acttgaatag cagaataaat catttctatg aatgttccgc cattagtcaa
    25441 tgacttcgct aaaatatata caatcataga agataagaaa attacaaatg ctggaatcat
    25501 tgcttcaaac tgtttggcaa tagcttgtgg aacttgttct ggcatcttaa taacaatttt
    25561 tctctttata aagaaggtat aaatacttcc tactaccaaa cctataatga tagcaccgat
    25621 aattccttgg cctccaaacc aaactttact aatagcgtcc ccaatcgcct caccttgttt
    25681 agggatataa gatgatctta gcaaaataaa gaatgcagat acagatagaa ctccagctgg
    25741 taaagcctct actccgctat tcttagcata agaataggca attgaaaaac aagaaattag
    25801 acccataata gcaaaagttc ctgaatatac ttgcataaac ggctctgtcc aattagctcc
    25861 aaaaacacta gcaatgctct tatttaatcc ttcgaacggc aattgtccca taatcaagaa
    25921 caaactacca actactgtca atggcaaaat tgctaacatc ccatctttta gagctataat
    25981 gccacgcata ttcacaaact tcatcatcgg tgcaatgatt ttctgaacat ccatctttga
    26041 cataataaat ctccttttct tacccactaa tcaaagatag ggccaaatct aatacttttt
    26101 tcccatctaa cataccatag tccatcatcg gaataacagc tatcggaaca tcacacttat
    26161 cacaaatttc ttttgattta tctaatgtat aagcaacttg tggacccaat agtgcaacat
    26221 ctatatttgg cgcataatcc gctaatttag actgagaaaa cgcctctatt tctgcctcaa
    26281 ctccactagc ttgcgctgca attttcatat tatttacaag cataccagta gaaaaacctg
    26341 ctgcacaaaa caaaccaatc ttcaccatta tgttttcctc ctctatgtta ataacaatga
    26401 taatactcta gtattatttt ttatgaagtt cttttctcaa actaataatt tctttgatta
    26461 aattaatctc cgtcatacta gtcatgagat gatcttgtga atgaacaaag agagctgtaa
    26521 tttctgtctt tgtaccagac gcttcctgtg ccaagaattt tgtttgtaga ttatgagctt
    26581 ctaataaggc ggcatcagct aactggattt cttcttcaca tcgttcacat gtaccgttct
    26641 ttatgtaatc taatgcttta taaatatgtt gtttcgcatc gccagcatat aaaattaaac
    26701 ccatgataat ttgatctgga acaataattt ccataaattc ctcctcattt cactatgtaa
    26761 aaagcttttt tcatttgaaa ttttaaaatt ttcttttgta actatagaat acaactacat
    26821 aataaagttg tcaatccaaa aacaacagat aaactatttt tgtttatata agcacaaatg
    26881 tgcaatcgac taaaaagaaa aaacgataaa ctgatcagtc tatcattttc actcacctag
    26941 aatctctcta aaagtcaaat caccatcttc ttccaaaact tttaaaattg tatttttatc
    27001 tgtaatcaaa tgatttacta aattagcacg caagacagaa agaatcgaag atactttcgt
    27061 atcaccgtaa gcaacagcta aactttgagg aatatttttt aaatcttcca aagagatggc
    27121 tatcgttctt tcctgtaagt tttcatagac ttctttacct tttgaatcaa aaaatcgaca
    27181 acaaatttct cctacagttt taactttggt caattcttta aagtcatcct ctgtaagcat
    27241 gtctaaccat tgatgtttcc ctttattgct aaaatcacca attccgacta cagctatatc
    27301 taaatctttc caactatttt tcaaattttc aaaatatctt gattgcaaaa taccatctgc
    27361 taacaattta ttttcttgca caatcgttgc attcataaat gtacactctc catgaaattt
    27421 tctagacatt tcataaatca gtgtattcac atggtattta gcgtgtatgt gactaggacc
    27481 acctgctaga ggatagaagt gaacatttcg gacactttta ctgtgaatta aatctactaa
    27541 attacttaaa cttttccccc aagaaaagcc aattttcata ttatcatcaa ttagattcct
    27601 aaggacgcct gctgcaactt gagaaattct ttcagataaa attgttggag tatcatcaaa
    27661 ttcatttgga ataatttcta aactttccaa actgtatttt tcttttacat aattttccaa
    27721 cttaaacata ttggtatcaa aattctctat ttcaatttta acaattccta cattccttgc
    27781 ttctgttaac attctactaa tagaggttct ataaattcct aattttgctg ctatttgtga
    27841 ctgatttaag ttttcaatat aatacagata agcaatttta gaaagcagtt tattcctatc
    27901 ttgattcata cacttaacct cttacgaaac taccttaacc attatcccag cattttctaa
    27961 tgtagctata ttttgtttag aaagtttttc gtctgttatt acttcataga cttgacttaa
    28021 agcaaatctt cttactgtac ctcttttatc aaatttactt gagtcagtta ggacaatgac
    28081 tttatccgac actgctgaaa tatattgaac tacctcactg cgcattaaat cttttccggt
    28141 aaagcccatc tctttatcgt aaccatctgt cccaacaaaa gcttgacaca catgaaaagt
    28201 ctgtatcatt tcttttaata aaggtcctac agtcacctgt gaatctttct gaaactcacc
    28261 accaagaaca ataacacgac atgaatcata agctctcaca aaatttgcta taaaaaacga
    28321 atttgttaca atcgtaacat ttcttttttg cttgcaaatt tcctcagcaa gtaaagcaca
    28381 ggtcgatcca gattctatca ttattgtttc attatctgac accaatttta ctgcttcctg
    28441 aacaattttt ctcttagttt cataattaat tgacaaacgt acatttaagt catctccact
    28501 atttaataca gcatatccat gctctctgtg taataaacct tttgactcta atttatctaa
    28561 atcttttcta atcgttactt tcgatacatt taatttttcc gataatgtat taacgtcgat
    28621 cttttcatat tctgatacta atttaataat ttgttccaat cttttcattt tacacctccg
    28681 ttttattcta ccaaaataaa aagcaaaaaa caacaaatta aactttcgtt cgtaattgtt
    28741 tttctttcgt ttttgtgata ggatagactt atgaagagga ggaactctta tggaaatatc
    28801 taaaggaatt atttttaata ttcaacactt ttcaattcat gacggcccgg gtattcgtac
    28861 aactgttttt ttaaaaggat gtcctctgcg ctgtccatgg tgttctaacc ctgaatctca
    28921 aagaatgaaa cctgaaaaaa tgaaagatgc tcaacgagag aaattcacct tagtcggtga
    28981 agaaaagact gtagaagaaa ttattacaga ggtattaaaa gacaaagaat tttacgaaga
    29041 atccggtgga ggtttaactt tatcaggagg tgaaatattt gctcagtttg aatttgctaa
    29101 agccatctta aaatcagcta aagaacatca catacacact gccattgaaa ctactgcctt
    29161 tgttgatcat gaaaaattta tcgatttaat tcaatatgtg gattttatct acacagacct
    29221 aaaacattat aattctataa aacataaaaa agtgactggg gtttttaatc aaatgattat
    29281 taaaaacatt cattatgctt tttctcaaaa taaaactatc gttttaagaa tcccagttat
    29341 tcctaatttt aacaatagtt tagaggatgc agaaaaattc gctactctat ttaactcatt
    29401 aaatatcgac caagttcaac tactcccttt tcatcaattt ggtgaaaaca aatatcgttt
    29461 attaaatcgg aaatatgaaa tggatggagt caacgcactt catcctgaag atcttattga
    29521 ttatcaaaag gtatttctga accaccatat taattgttat ttctagttca tttccttgaa
    29581 acgctctagc tatttgcaga taacaagcat ctatataagg ttatcaaaga agacttcccc
    29641 atgactctaa atgaatatat cttacaatat cgcctgaaac aagctataga taagatggct
    29701 gaatctccca actcctcttt aagcgatatc tctgaccaag ttggattttc agactataaa
    29761 tattttgcca aagtatttaa aaagtatctc catatttccc caaaaaaatt gaaatcactc
    29821 ggaagaatag taaaataggc tattcttctt tttgtagaga attttactct aatatagtga
    29881 attgaaataa gatgtgaaca tctctatcag gaaagtcaaa ttaatttata gaaatatttt
    29941 agcagtcaag gtgtactatt atagattcaa tgcactatag aatagaattg caggttttaa
    30001 aaacgcttac attgttttaa aataaactta aataacacaa cggaggtatc catcatgaaa
    30061 aagaaatgga tgtattatgc tgcttgttct tctaatgaat ctgccgatga cagttcatct
    30121 gataaaggag acggtggttc gctagtcgtt tattgaccaa actcagaagg cttaattgga
    30181 gcaactattc ctgcctttga agaaaaatat ggtatcaaaa tagaactgat tcaagctggt
    30241 actggagaac ttttcaaaaa actagagtca gaaaaagaag ttcctgtagc tgatgttatc
    30301 tttggtggtt cttatacaca atatgctacc cacggagaac tctttgaaaa ctatatttca
    30361 aaagaaaatg ataatgttat caaagaatat caaaacacaa ctggcttctc tactccttat
    30421 acactagatg gtagtgtttt aatcgtccac cctgatttaa ctaaaggcat gaacatcgaa
    30481 ggatatagcg accttctcaa acctgaacta aaaggaaaaa tcgcaactgc tgacccagca
    30541 aactcttcta gcgcctttgc tcaattaaca aatatgctac aagctcaagg tggttacaaa
    30601 gatgataagg cttggtctta tgtaaaagat cttttcacac ttattgatgg tatagtaaaa
    30661 tgaaataaga acaggacaag ttaatcagga cagtcaaatc gatttctaac aatgttttag
    30721 aagtagatgt gtactattct agtttcaata tactataaaa tcggtttaag ttcatctggt
    30781 gtctataaag cagtcgctga tggagaaatg actgttggtc tctcttataa agatccagca
    30841 gttaaactct taaatgacgg agctaacatt aaggtagtct atccaaaaga aggaaccgtc
    30901 ttcctacctg ctagtgctgc tatcgttaaa aaatctaaaa atatggaaaa tgccaagaaa
    30961 tttatcgatt ttattatctc tcaagaagta caagatacac ttggtacaac cactactaac
    31021 cgtcctgttc gtaaaaatgc taaaacaagc gaaaacatga aaccaattga caaaatcaaa
    31081 acactcactg aagattatga ttatgtcatc aagaataaat cagatatcgt taagaaatac
    31141 aacgaagtct ttacagatat ccaatctaaa cagtaaaaga ggttcactat gagtgagatc
    31201 aaaattatta acgccaaaaa aatctaccac gatgtccctg ttattgagaa tttgaacatt
    31261 acaattccaa aagaaagtct ctttaccctt cttggacctt caggatgtgg gaaaacaacc
    31321 cttcttcgta tgattgcagg tttcaacagt atcgaaggtg gagaatttta cttcgatgat
    31381 acaaaaatca ataatatgga acccagcaaa cgcaatatcg ggatggtttt ccaaaactac
    31441 gctattttcc cacatttgac tgtccgagac aacgttgctt ttggtcttat gcaaaagaag
    31501 gttccaaaag aagaattgat tcaacagacc aacaagtatc ttgaactcat gcaaattgct
    31561 caatatgcgg atcgaaagcc cgataaactc agtggtggac aacaacaacg tgtcaccttg
    31621 gcatgcgcct tagcggttaa tccaagtgtt ctcctcatgg acgagccact tagtaatctg
    31681 gaggccaaac ttcgcttgga tatacgtcaa gccatccgag aaatccaaca cgaagtggga
    31741 attacaactg tttatgtaac ccacgaccaa gaagaagcca tggctatttc agaccaaatt
    31801 gctgttatga aagatggggt gatccaacaa atcggccgac caaaagaact ctatcataaa
    31861 ccagctaatg agtttgtggc aacctttatc ggacgcacaa atattatccc tgccaatctt
    31921 gaaaaacgga gcgacggcgc ttatatcgtc ttttcagatg gctatgccct tcgaatgcta
    31981 gctcttgatc aggttgagga gcaagctatt catgtaagca ttcgtcccga agagtttatc
    32041 aaagatgaat ctggagatat tgaaggaact attagcgata gcgtctatct tggactaaat
    32101 acggagtatt tcattgagac aggttttgcc tcaaaaattc aagttagtga agaatcaact
    32161 tttgaagaag aactacaaaa aggcaatcgt attcgtctac gaatcaatac gcaaaaatta
    32221 aacatctttt ctgcagatgg ttcccaaaac ctgataaaag gagtcaacca tggaacgtaa
    32281 aaaactaaat atttggacag cctcctcttt cttcatcttt cttacctatc ttgtctttct
    32341 cgtttatcct atcgttaccg tgctcaagta agcacttata catgaaggat aattctcact
    32401 agctaatttt gtcactttct ttagtaaaac ctactactct gagacactag tcaacagttt
    32461 cagggtttcc attaccgcta ctgtcacttc cttagttgta ggaaccctat tagcttatct
    32521 cttctctatg tatgacttca aggggaagaa atttctacaa atattgatta tcattgcttc
    32581 catgtcagct cctttcgtag gagcctactc ctggattctc ttgctgagac gaaatgaggt
    32641 catcactaaa tttttgacaa atgcccttca tcttccagct atcgatattt atggattcaa
    32701 aggaattata cttgtcttta cactgcaact attcccactg gtatttctat atgttgctgg
    32761 aacaatgaac agtattgaca attctctact tgaagctgct gaaagcatgg gatccttcgg
    32821 atttaaacct atcgtaacgg ttgttttacc tctcctagtt ccaaccttac tagcagcccc
    32881 ttgcttgtat ttatgagagc attctcagac tttggaacgc ctatgttgat tggcgaagga
    32941 tatcggactt tccctgtcct gatttatacc caatttatta gcgaggttgg aggaaattct
    33001 gcttttgcaa ttatggcgat tatcattgcc ttggcaattt tccttatcca aaaacacatt
    33061 gcaaaccgct acagtttcag catgaatccg ctccatccaa ttgagcctaa aaaaactaca
    33121 aaaggaaaaa tggctgccat ttatgcaaca gtctacggaa ttatctttat ctctgtttta
    33181 cctcaaatct acttaattta tacctctttc ctaaaaacat caggtatggt atttgttaaa
    33241 ggttattctc caaacagtta caaggtagct ttcaatcgta tgggatctgc tattttcaat
    33301 accattcgta tccctttgat tgccttagtt ctagttgttc tatttgcgac atttatctcc
    33361 tacctagccg ttagaaaacg gaatttattt acaaacttaa ttgacagcct cagtatggta
    33421 ccttatattg taccaggaac cgttctaggg attgccttca tttcttcctt caatactggt
    33481 ctatttggaa gtggatttct tatgattaca gggactgctt tcatcttgat tatgtctcta
    33541 tctgtcagaa gattaccgta tactattcgc tcatctgttg ctagcttaca acaaatagca
    33601 ccaagtattg aagaagctgc tgaaagctta ggaagtagtc gtctcaatac ctttgctaag
    33661 attacaactc caatgatgct atcgggtatt atttctggag ccatcctatc ttgggtcaca
    33721 ctgatttcag aactctctac ttctatcctc tatcctcctc tacaatgtca aaacaagaac
    33781 aatgactgta gctatttata cagaggttct cagaggaaat tacggtgtag ccgcagcctt
    33841 gtcaactatc ctgactgttc taacagtagg ttccttgctc ttgtttatga aaatctctaa
    33901 aagcaatagc attacacttt agttttccac atcaaaaaca gccgaaagga gtaccctcgg
    33961 ctgttttttc ttatcttgat attcttatca aatccctagc tcatggcaag caagtctaga
    34021 ctgattaaat agagccttct tccatcttct cacgtcctag ctctcggtaa ctacggtcac
    34081 cgacaacttc aaccctaaac tgaccgtcct catattcaag aatcgtcacg ctaccattat
    34141 ctagaccatg cggatgcatg ccattaatca gataaacaat ggttccaata gtcattccat
    34201 ggctgacaac aagggcgtta cctccacctt gatcttccat ttcttttgca attatttcaa
    34261 agccttcctt gattcggcca ctgagttttt cccaaccttc agcccaacca gctgtatcga
    34321 cctctaccaa gccctcagcc agttcagcat aagacaattg gtgaacgtgg tccacattaa
    34381 agatacgagg aataatgccc atgaaaagat cgccatcata ggctccatca aaactaccga
    34441 aacaccattc tctgatacgc ttgtccatgc gataagggat tttcccctgc aagccaagtt
    34501 cttcaagtat aattcccatg gtctgaatgg tacgaccaga atcactcgaa taagcgcgct
    34561 caaactgtag atcagattct cgcaaaccga ttcctaactc ttgaatccct cgttcacctt
    34621 cagcagttaa gggagtatcg ctccaacctt gcgcgcgacc aatcgtgtta aacatggtct
    34681 tgccatgacg taccaaatac aatcgtactt ttaccatttt ccgtcctccg tttgcttcta
    34741 ttatatcatg aatgataaaa taaaagccac ccatacaggc gacttttgaa ggagattatt
    34801 atgaaaaagt ttaggagttc attaaataaa gatattagat gaaaatcaaa ttcaaactaa
    34861 ttcagtgtta tctgtttcaa ataatattag gaggtcttta tttaatgatt atagtataca
    34921 catctaatct taaatagaac ttaaaatttc tcctattttc ttaattttta aaactatgaa
    34981 ttggtgctgg gatttgtcca ccgcgagaga tgaagtcgac agacgaagcc ccattaacct
    35041 tcataacggg tgcagttcct aatagaccac caaactcaat catatcgcct tctcttcctt
    35101 tgggaatgat acgaacagct gttgttttca tgttgataac accgattgct gcttcatccg
    35161 caatcatagc cgcaatagtt tcagcaggcg tatcttctgg gatggcaatc atatccaatc
    35221 caacagaaca gatagccgtc atagcttcta gtttttctaa attaagagag ccattttgca
    35281 ctgcagcaat cattccttca tcctcagaaa cagggataaa ggcaccagat agaccaccga
    35341 cctggttaca ggccatcact ccaccctttt taacttggtc gttcaagagg gccaaggcag
    35401 ctgtcgttcc atgcgtgcca actgtttcta gccccatttc ctcaaggaca cgtgccacag
    35461 agtctccaac cgcaggggtt ggtgccaaac tcaagtccac aataccaaac tccacaccca
    35521 gtctctcact ggccatttga ccaaccaatt gaccgatacg agtgatttta aaggcagttt
    35581 tcttaactgt ttcggctact acatcaaagc tctgtccacg aactttttcc aaagcacgtt
    35641 tcacaacacc aggaccagaa actccgacat tgatgataac atctgcttcc ccaacaccat
    35701 gaaaggcacc cgccataaat ggattgtcct caacagcatt agcgaataca accaacttgg
    35761 ccactcccat atctgaaaga tttgctgttt ccttgataat tcgtcccata tctgccacag
    35821 ccgtcatatt aataccagac ttggttgagc cgatattgac tgacgagcag accttatccg
    35881 tctcagccaa agcgcgagga atggaattga tgagaatctc atctcccttt tgataacctt
    35941 tttgtactaa ggcagaaaaa ccaccaataa agtccacacc aatctctttc gcagccttat
    36001 caagcgcttt tgccagaacc acgtagtccg tcgcatctgt cgctgcccca atcagagaaa
    36061 taggtgtcac cgatacacgc ttattaacga taggaattcc caactcagcc gcaatttcat
    36121 caccaacagc tactaaatta gccgcctttg tcgtaatttt ttgatagatt ttctccgcag
    36181 cacaattgat atctggatcg atacagtcca aaagagaaat ccccatggta atggttctaa
    36241 tatcgaagtt ttgctcctca atcatggcga tggtttcagt aacttgtcta atatccatga
    36301 tgacctccta gatattatac atagcttcga aaatcgctgc actctgaata ttgattttta
    36361 cattcaaagt ttgcccaaaa gcttcaaatt cattacgaag ataggtaaaa tcttgctttt
    36421 catcactaga tacaacagcc atcatcgtaa aatattcatc taagacagtt tgagagatat
    36481 cgtcaatatt caatcctaat tctgcaattt taccagaaac acctgcaaca attccagatt
    36541 tatctttacc aacaacagtt ataatggctt tcataagtaa actccaattc ttcttaaaat
    36601 aagaaacctt aacattaaac aggattcttt tcaaatagta tagcataatt ctaacgaaaa
    36661 tactcaaaaa cgcctgctta cgaagttgtt cttaagaaaa aaacaatttc gtaaacaagc
    36721 gtctactatc ccaacttatg atgagtgttc ccgctaggac cgaaatccta gcggtagacc
    36781 agagctagac taagagcaca agactccatc atcataacac tcaacaaaat tgatgatttt
    36841 atactaattc gatgatcgcc attggcgctg catcaccacg acgtggttca gttttaagga
    36901 tacgagtgta tccaccgtta cgttcagcat aacgaggtgc gatttctgag aacaattttt
    36961 gaagtgctgt agtagaagtg tacttatcag ttgcttcatc atagttttca gatgcgattt
    37021 cattacgtac gaaagcagct gcttgacgac gtgcatgcaa atcaccacgt ttacctagag
    37081 taatcatttt ttcaacagtt ttacggattt ctttagcacg agcttcagtt gtcacgattg
    37141 attcgttgat caaaaggtca gttgtcaaat cgcgaagcat tgctttacgt tgtgagctag
    37201 tgcgtcctag tttacggtaa gccatgtatt cctcctttat ttatctttta atccaagacc
    37261 caaatcaatg agtttgagtt tcacttcttc caaactcttg cgtccaagat ttcgtacttt
    37321 catcatctct gcttcagatt tttctgtcaa atcatgcaca gtattgatac cggcacgttt
    37381 taaacagttg tatgaacgca cagacaagtc cagttcctca atcgtacgat ctaaaatacg
    37441 gtcgtcagat tcagtatcag cttctttcat cacttcagtt gacttagcaa tctcagtaag
    37501 atttgtaaac aaatcaagat gttctgtcaa aatacgtgct gaaagcccta aagcatcttc
    37561 tggaataatt gttccatttg tcaagatttc aagggttaat ttgtcgaaac catcattgct
    37621 acctacacga gcaggttcca cttgatagtt gacttttgta actggtgtat aaatagaatc
    37681 tacagcaagt gttccaactg gtgcattatc ctttttattt tcatcagcag gtacatatcc
    37741 acgaccactg ttaacagtca tagtcgcttt tagagaagaa ccttcaccga ttgtaaagag
    37801 ataatgatct ggatttacaa tttcaatatc gctatctgtc aaaatgtcac cagctgttac
    37861 ttcagcagga ccttcaacat ccagttcgat gattttttcg tcttcaacgt acgatttcac
    37921 tgcaattcct ttaatgttca gaatgatttg catcacgtct tcacgaacac ctggaactgt
    37981 gtcaaactca tgtaacacac catcaatgtt gatagatgtc acagctgctc ctggtagaga
    38041 agctagaagt acacgacgaa gagagttacc aagagttgta ccgtagccac gttcaagtgg
    38101 ttcgattaca aacttgccat aatctttatt ttcatcaatt tttgttatat ttggtttttc
    38161 aaactcgatc atttagttac tccctcttaa acgaaaagca gtgtaatgcg atgattatac
    38221 acggcgacgt tttggaggac gagcaccatt gtgtggcact ggagtcacat cacgaattgc
    38281 tgttacttca agaccagcgg cagcaagcgc acgaatagct gactcacgac cagaacctgg
    38341 accttttaca gtaacttcaa ctgatttaag accgtgttct tgtgcagatt tagcagcagc
    38401 ttcagaagcc atttgagcag cgaatggtgt agatttacga gaacctttga aaccaagagc
    38461 accagctgat gaccaagcaa ttgcattacc atgcacatca gtaatcataa caatagtgtt
    38521 attaaatgta gcgtgaatat gagcaatacc agattcgata ttctttttca cacgacgttt
    38581 acgtgttggt ttagccaaga cttttacctc ctatattatt ttttcttacc agcaatcgca
    38641 acagctttac ctttacgagt gcgggcgttg tttttagtgt tttgtccacg gacaggaagt
    38701 ccacgacggt gacggatacc acggtatgaa ccgatttcca tcaaacgttt gatgttcaag
    38761 tttacttcac gacgaaggtc accttcaact ttgattgcat ccacttcacg acggatagca
    38821 tcttcttgat ctgatgtaag atcacgtaca cgaacatctt ctgagattcc agcagcagcc
    38881 aaaattttct tagatgttgc aagtccgata ccataaacat aagtcaatga gattactacg
    38941 cgtttgtcat ttggaatatc aactccagca atacgagcca tgttttctcc tttctatctt
    39001 atccttgacg ttgtttgtgt tttggatttg ctgggcaaat taccataaca cgaccattac
    39061 gacgaataac tttacagtat tcgcaaattg gtttgaccga tggtcttact ttcatttctt
    39121 atccctccaa gtttttcgat tatttaaagc ggtaagtgat acgtccacgt gtcaagtcat
    39181 atggactcat ttcgacagta acacgatctc ccgctaaaat acgaatatag tttttacgaa
    39241 ttttaccaga aactgttgct aaaatctgat gtccattttc aagttcaacc gtaaacattg
    39301 cattcggcat tgtatcaact actttgcctt caacttcaat cacatcgtct tttgccacgc
    39361 aaaagcacct ccataaattt cgattcgatg cctctagaca cagagacaac aattataagt
    39421 cagactatct cagtataaca tttgtagcgg atttttgcaa gtgcgaaaaa cgctttattt
    39481 caaatttgtc aatacttttt caatatctga gaagacatca ttgatatctt gattaccttc
    39541 gatgtcatga accaaacctt tggcacggta gtgagcaatg attggttctc cttgagcaat
    39601 attaacatcc aaacgacgtt ttactgtctc aggcttatca tcttcacgtt ggtagtaatc
    39661 ttcttcttta tagtcaactg gtgggttaaa gaccttgtgg aaagtttctc cagttacgcg
    39721 gtggatgata cggccactca aacgttccaa gaggctgtca gggttcactt caatattgat
    39781 aataccttct agttcaatgc caagttcagc caatgttttg tccaaggcat gagcttgttc
    39841 aattgtacgt gggtaaccat ccaataagaa tcctgtttct ttaatatcat cttgtgaaag
    39901 gcgttctttt acgattccat ttgtaacttc gtcaggaacc aattcaccct tgtcaatata
    39961 tgacttagca agaacaccca tttcagtttg atttgccatt gcagcgcgga acatatcacc
    40021 tgttgagata tgtgcaacat ggaattgttc tacgattttt gctgcttgag ttcccttacc
    40081 tgcaccaggt aagcccataa tcaaaagatt catgatttga tctccttatt ttatttttaa
    40141 atagaagcaa aaagtctttt cacccctatt taaaaacaaa atagaagagg ggagatccaa
    40201 ctcttacaaa tgtcagagtt taaacctcca ctccctcagt atttactgat tcagtaaata
    40261 cttttattct gttctgtcca tgaaaccaac atacttacgt ttcaataggt aaccttccaa
    40321 ttgcttgatt ccttcgatac ctgtagagat aatgatcaag agacttgttc caccaaaggc
    40381 aacaacatca gaaagaccaa atacatcttt agctgcaatc ggtaaaatgg aaatcacacc
    40441 aaggaagagg gaaccaacag ttgcaagacg acgaagaagt ttagacatat attcttctgt
    40501 acctttacca ggacgaactc catggatata ggcaccactc ttttgtaggc tctctgctgc
    40561 tttttcagga ttaatctgta caaacgtata gaagaatgta aagagaataa tcaacaaagc
    40621 atacatggca ataccagttg gagaagtagt tgccaacatc tcttgtgcta cccttaccca
    40681 agcccaatca tgacctgtgg cactcaaaaa ctgaagaata gccgcaggcg ctgcagtaat
    40741 cgaactggca aagataacag ggataactcc agcagggttt acctttaacg gaaggtaaga
    40801 gctagatgga gcaccttgtg caaccttagt atattggatt ggaattttgt attctgcttg
    40861 ttgaacataa gttgtaaagt aaataatcaa caatacagta ataatcaaaa tgattacgaa
    40921 aatgatagat gaagtgatac ggctacttgg gacgttcaca aagtagtcca catagatgcc
    40981 ctgaatcatc tctggaattg aggaaacaat cccggcaaag ataatcatgg aaacaccgtt
    41041 tccgtatccc ttatctgtaa tttgctcacc caaccaagtg acaatcatac taccagctgt
    41101 taagatgata ccaatcgtca gaaaaacttg tggagttaaa gcagttttaa tcaattgagc
    41161 tccagccaag gtattaaaac cagctgtaat cccgatagat tgcacaaaag cgagaactag
    41221 agcaatataa cgagtagctt gattcaattt tcttcgacct acttcccctt gtttacccca
    41281 ctctacaaac ttgggtaaaa tatccatttg caagagttgg acaacaatag aagcggtgat
    41341 atagggacta actcctaggg caaaaatcga aaagtttttt agggcattcc ccgacaccaa
    41401 gctcaacatg tttaagaagg ataatccact taaagcattc aagctattgg cattcacacc
    41461 aggaactgta atgctagttc cgatacgaaa gaccaaaacg ataaaaattg taaataaaat
    41521 ttttgatcga acctgcttga ctttaagagc ttctcttaat aatttaaaaa acataggtca
    41581 cctctcttag atgacttcta ctgaaccacc tttagcagtg atagcttctt cagctgattt
    41641 agagaattta gctgctttca cagtcaattt cttagtcaac tcaccgttac caagaatttt
    41701 aatacctgac ttttcagctt taacaattcc tgcttcgata agaacaactg gagttacttc
    41761 agcaccatct tcaaagacgt tcaattggtc aaggttcaca attgcgtatt ctttagcgtt
    41821 gatgttagtg aatccacgtt ttggaagacg acggaacaat ggagtttgtc caccttcaaa
    41881 accaaggcga actccgccac cgctacgagc tttttgacct ttttgaccac gaccagatgt
    41941 tttaccgtta cctgatgaag taccacgacc aacgcggtta cgtactttac gagaaccttc
    42001 tgcaggtttc aattcatgaa gtttcatttt tattttctcc tcttttgtaa aatgctagcg
    42061 ccgataaggg agaaaaggtt gtctccccca tcaactcgcc tatacgatgt catcatagat
    42121 gactatatct agttttaggg gatggtacag tgcacatccc ctaaaacttc attagtttac
    42181 ttcttcaact gttactaagt gagatactgc tgtaatcata ccacggatag cagcgttatc
    42241 ttctttaata acagagctgt tcaatttgcc aagtccaagt gctacaacag ttttacgttg
    42301 tgatggaatg cgtccgattg gagacttagt caaagtaatt ttaatttgag ccattttatc
    42361 ccctttctta tgccaaatca gaaactgaaa taccacgaag ggcagcaatt tcttcagcgc
    42421 gtttcaattg tttcaaacct tcaacagttg cacgaacaat gttgattgga gtgttagaac
    42481 caagtgattt agatgtaata tctgccacac ctgccaattc cacaacggca cgaactgcac
    42541 caccagcggc aactccagaa ccttctacag caggtttcaa caatacttta gctccaccga
    42601 attctgaaag aacttcgtgt gggattgttg ttccaaccat aggaacttcg atcaagtttt
    42661 tcttagcatc atctactgct ttacggattg cttctggaac ttcttgagct ttaccagtac
    42721 caaatcctac gcgaccattg tggtcaccaa caacaacaag agctgcgaaa cgaagacgac
    42781 gtccaccttt aacaactttt gtaacacggt tgacagcaac tacgcgttct tctaattcaa
    42841 ctgcattgtc tttaaatgcc attttctagt gtcctcctat tagaatttca atccgttttc
    42901 acgagctgca tcagccaaag ctttcacacg tccgtgatat agatatccac cgcggtcgaa
    42961 caccacttct gaaatacctt tagcgtttgc acgttctgca acgagtttac cgacagcaac
    43021 ggcttgttca gttttagttc cttttgaaac ttctttatca agagttgaag cacttgcgag
    43081 cgttacaccc gctacgtcat caatcacttg agcgtagatg cctgtattag aacggaatac
    43141 gttcaaacgt gggcgatcag cagttccaga gagttttccg cgaacgcgac ggtggcgttt
    43201 ttggcggagt ttgtttttat ctggttttga aatcacagtt ttcacctctt tagttttaaa
    43261 tcgtgtgcta tgcacaaagt tggaaaatag gttggtggtt gatgatcaac cactcaacat
    43321 tatttacctg ttttaccttc tttacggcga acgaattcac caacgtaacg gataccttta
    43381 cctttatatg gttctggtga acgaaggcta cgtacgtaag cagctgtttg accaactact
    43441 tcttttgaaa ttccgctaac aacgattgtt gttgggtttg gaagttcaaa agtaattcct
    43501 tctggagctt caacttcgtc tggatgagat ttaccaacag ccaaaacaag tttagatcct
    43561 tgaagctgtg cacggtaacc aaccccacgc atttcaagtt ctttcttgaa tccttctgat
    43621 acaccaacaa ccatgttgtt caaaagggca cgagtagttc cgtggatagt tttcatttct
    43681 tttgaatcgt ttggacggtg aagagttatt tcagtacctt ccacacggat ttcaatatct
    43741 tttgagaact cacgagtaag ttctccttta ggtcctttta cagttacaac gttgtcattg
    43801 ttagcgagtt caacaccagc aggcaacacg ataactttat taccaatacg tgacatgttt
    43861 attttctcct gttaaattgt caggccagaa cggccagttt tcacggggtt cagatactta
    43921 tttagttcaa gagctgaact gaacacgctc taagaactgt gcaaaataga taagttggct
    43981 tgtttgtaaa caaacttctc caacttccta attttgcttt gttctttacg agctttgtat
    44041 cttgatttta ccaaacgtaa gcgataacct caccaccaac attcttttgg cgtgcttctt
    44101 tatcagtaag caaaccttca gaagttgaaa ggatggcaat tccaagtccg ttaagaactt
    44161 ttggaaggtc ttcacgtttt ttgtagacac gaagtcctgg tttagaaaca cgtttcaagt
    44221 tagtgataac tttctcacca tttggtccgt atttaagaaa tacacggatg acgccttgtt
    44281 tgtcatcttc aatgatttca acgtttttta caaaaccttc gcgtttaagg atttcagcaa
    44341 tccctttttt gatgtttgat gcaggtactt caagtacttc gtgtttagct tggttagcat
    44401 tacgaatacg agttaggaag tctgcgattg ggtcagtcat aaccattttg tttttctcct
    44461 cttactagta gtttgcaagt tgcacttgct agttaataca tgatacaaag agcgtaacag
    44521 cacgagcaaa aatagacaat ttaatgcaga agcaatgctc caaagcaaat tggtcttttt
    44581 tgccaaagct gtagctcgtg ttcaaattgg caagcccaat tgaacccggg ctaaactctg
    44641 tgtgaaaaag ataaactttc ctagaaactt tagtttcttc gtcaagtttc ctatttttac
    44701 ttggagtttt gacgctcttg atatcttaaa ttaccaagat gcttttgtta caccaggaat
    44761 ttgaccttta tgcgcaagtt cgcgaaaagc gatacgactc agaccaaatt tgcggtaaac
    44821 tgaatgtggg cgccccgtaa ctctacaacg attatgtaaa cgagtcggtg aggcgttgcg
    44881 aggtaattta gataaacctt cgtagtcccc tgccgccttt aatgcagcac gtttttcagc
    44941 ataacggtca acaatttttt ggcgtttagc ctctctagct accattgatt ttttagccat
    45001 tagatttacc tcctatatta ttttgcaaaa ggcattccaa ggcctgtaag caatgcacgt
    45061 gactcttcgt cagtgttagc agttgttacg ataacgatgt caagaccacg agttttgtca
    45121 acgtcatcga agttgatttc tgggaagatt aattgttctt tcacaccaag tgtgtagttc
    45181 ccgcgtccat caaatgattt tgttggaaca ccgtggaagt cacgtacacg tggaagtgaa
    45241 actgatacca atttatccaa gaattcgtac atacgttcac cacgaagggt aacttttgca
    45301 ccgatcgcaa caccttcacg aagacggaag ccggcgattg attttttagc tttagtgata
    45361 agtggttttt gacctgagat aagtgccaat tcttcagcag ctttttcaag gcttttagcg
    45421 tttgatacag cttcaccaac acccatgttc aaaacaatct tatctacttt aggcacagcc
    45481 atcactgatg agtagttgaa ttgttctgtc aaagcaggaa ctacttcatt aagatatttt
    45541 tcttttaaac gatttgccat tatacttctc ctttccttcg tgattaatca agcacttcgc
    45601 ctgatttttt gttgtagcga acttttttac cgtctacaaa tttgtaacca acacgaccag
    45661 ctacaccatt tttgtccaaa acttgaacgt ttgatacgtg gatagctgct tctttctcga
    45721 tgataccacc ttgaggaagc tcgttagttg gacgttggtg tttcttaaca atgttaacac
    45781 cttcaacgat aactttgttt acttttggaa gggcagtaag gacaacagct tctgttccct
    45841 tatctttacc agcgattacg cgaactttgt cgcctttttt tacaaacatt aggtttctcc
    45901 ttgatttttc ttacgcccat aagggcaccc tagcttgaag ctaggggact agtttgttcc
    45961 taaaaattaa agtacttctg gagcaagtga cacgatcttc atgaagccac cttcacgcaa
    46021 ttcacgtgca actgggccaa agatacgtgt tccgcgagga gttttgtctt cacggatgat
    46081 aactgctgcg ttttcgtcaa atttgatgta tgaaccatca gcacgacgag cacctgattt
    46141 agtacgaacg ataactgctt taacaacgtc acctttttta accgcaccac caggagtagc
    46201 ttgttttaca gatgccacga taacatcacc gatgtttgca aatttacgtc ctgaaccacc
    46261 aagaactttg atagtcaaga tttcgcgagc accgctgttg tctgcgactt tcaaacgagt
    46321 ttctgtttga atcatttcag ttttctcctt tcaggtttga ttagatgatg accgcttctt
    46381 caacaacttc tacaagacgg aaacgttttg tagctgaaag cgggcgagtt tccatgatac
    46441 gtacgatatc gccttctttg gcaacattgt tttcatcatg agctttgtat tttttagagt
    46501 agttaatacg tttaccatag actgggtggt tacgttttgt ttcaactaca actgtgattg
    46561 tcttgtccat tttgtcagat acaacacgtc caacaagaac tttacgatta ttgcgttcca
    46621 ttgaaatttc tccttcccta gtctattatt tcgcttcaga ttgaactgtt ttgatacgag
    46681 cgatttgttt tttaacttct ttcaagcgag ctgtttgttc caattgacca gtagcagctt
    46741 ggaaacgaag ttcaaacaat tcttttttca attcgttttc gcgcttcgcg agttcttctt
    46801 gagaaagacc acgaagttct ttaacaaatt cttttacttc attaagtttc atgccttctc
    46861 cttattctgc ttcacgtttt acgaatttac atttaactgg caatttgtgg ctagcaagtc
    46921 gaagcgcttc acgtgcaatc tcttcagata caccagcgat ttcgaacatc actttaccac
    46981 gtttaactgg tgctacccaa ccttcaggtg cccctttacc agatcccata cgcacaccga
    47041 tagctttagc agtgtatgat ttgtgtggga agattttaat ccaaacttta ccaccacgtt
    47101 tcatgtaacg agtcatggcg atacgagcag cttcgatttg gcggttagtg atccagtggc
    47161 tagttgtagc ttgaagaccg tattcaccga atgctacttc ttttccacct tttgcttcac
    47221 cgcgcatttt tccacggaac tcacgacggt gtttaacacg tttaggtact aacattggtt
    47281 atttacctcc tttagtgttt ttacgagctg gaagaacttc accacggtag atccatactt
    47341 taacaccaag tttaccgtat gtagtatctg cttcttccca agcgtaatcg atatctgcac
    47401 gaagtgtgtg aagcggaaca gttccttcag agtatccttc agcacgggcg atatctgcac
    47461 cgttcaaacg acctgatact tgagttttga ttcctttagc tccagcacgc attgcacgtt
    47521 ggattgcttg tttttgtgca cgacggaaag caacacgttg ctccaattga cgagcaattc
    47581 cttcacctac aaggtgagca tccaaatcag gttgtttgat ttcgatgatg ttgatgtgta
    47641 cttgttttcc agtcaatttg ttaagttttg cacggagtgc atcaacgtta gcaccacctt
    47701 taccgataac catacctggt ttagcagtgt gaagtgaaac gttaactttg tttactgcgc
    47761 gttcgatttc aatagttgaa actgctgcgt cagcaagttc tttttgaacg aatttacgga
    47821 ttgcaagatc ttcatgaagg taatccgcgt attctttttc agcataccat ttggcatccc
    47881 aatcacggat gatgccgaca cgcataccaa ttggatgtac tttttgaccc acgattttac
    47941 ctccttattt ttctgcaaca gctacagtga tgtgagctgt acgtttgttg attggtgaag
    48001 ctgaaccttt cgcacgtgga cggaaacgtt tcatagttgg tccttcgttt gcgaatgctt
    48061 cagatactac caaattagct ttatccaaac caaagttgtt ttcagcgtta gctacagctg
    48121 agttcaaaac tttcaagatg atttcagcag ctttgtttgg agtgaatgtc aagattgcga
    48181 ttgcatcggc tacgctttta ccacggatgt tatcaagaac aagacgtgat ttacgaggtg
    48241 aaacacgtac tgtacgagcc attgctttag ctgaagtaat ttctgccatt tatgttctcc
    48301 ttattttcta cgtgttttct tgtcgtctgc agcgtgacct ttgtaagtac gagttggtgc
    48361 aaattcacca agtttgtggc ctaccatgtc ttcttggatg taaacaggta cgtgtttacg
    48421 tccgtcataa actgcaatag tgtaaccaat gaaacttggg aagatcgttg aacgacgtga
    48481 ccaagtttta ataacttttt tcttttcgtc gttagcttga gcttcaactt ttttcatcaa
    48541 atgctcatcg acgaaaggtc cttttttaag actgcgtccc atttttatat tttctccttt
    48601 aaatgttgta ccacagcggc ttgcactcct atggagcgct accgagctgg cggatttact
    48661 agttgcttaa gcgactagtt taatattatt tctcgttgcg acgacgaacg ataagtttgt
    48721 cagatttcgc tttcttgtta cgagttttaa gaccaagagc aggtttgccc catggagtag
    48781 atggtgcttt acgaccaact ggtgctttac cttcaccacc accgtgtggg tgatcgttag
    48841 ggttcattac agaaccacga actgttgggc ggataccttt ccaacggcta cgtcctgctt
    48901 taccaaggtt tacaagtcca tgttgttcgt ttccgacaac accaactgta gcacggcaag
    48961 ttccaagaat catacgaact tcacctgatt gaagacgaac aagaacatat ttaccttcag
    49021 aacccaatac ttgagcagat gcaccagcag cacgtaccaa ttcaccacca cgacctggtt
    49081 tcaactcgat gttgtgaatc aaagtaccaa ctgggatgtt agcaagtgga agagcgtttc
    49141 cgactttgat atctgcttct ggacctgaaa cgatacgttg acctacttca agaccttttg
    49201 gagcgatgat gtatgctttc acaccgtcag tgtagtgtac aagagcgatg tttgcagaac
    49261 ggtttggatc gtactcgatt gttttaacaa ctgcttcaac gttgtcttta ttacgtttga
    49321 agtcaaccaa acggtaaaaa cgtttgtgtc caccaccttg gtgacgaact gtgatacgac
    49381 cgttgttgtt acgaccagcc ttgctcttca atgcaacaag caatgatttt tcaggagtgc
    49441 ttgttgtgat ttcagcgaaa tccaaagaag tcatattacg gcgaccgttt gttgttggtt
    49501 tataaacacg aattcccacg atatttcctc cttagattat tcagcttcag cagcaaacaa
    49561 ctcgattgct ttagaatcag ctgtaagtgt gatgatagct tttttagttt tgttagtaaa
    49621 accagtgtaa cgtccaacac gtttagcttt tggttttacg ttgattgtgt taacattggc
    49681 aactttaaca ccttcgaaag cagcttcaac agcttgcttg atcaaaagtt tgtgtgcacg
    49741 agtgtcaact tcaaatacat attttcctgc ttcaagttga gccattgagc tttcagtgat
    49801 gacaggtttt ttgataacat catacaaatt cattatgcaa gaacctcctc gattttagag
    49861 atagctgctt gtgtgacaag aagtttgtcg ctatttgcga tgtcaagaac acttgcagtt
    49921 gtagcagttg caactttcac gtttggaagg ttacgagctg aaagagctgc gaattcattt
    49981 ccttcttcaa ggataacaag aactttagaa tcgatgctca atgctgcaag aacttttgca
    50041 aattcagcag tttttggagc tgtaaatgaa agagcgtcta cagctacgaa tttgttttca
    50101 gcaacttttt cagagtaaac tgatttaaga gctaggcgac gaactttttg tggaagtttg
    50161 tagccgtatg aacgtggagt tggtccgaag acaacaccac caccacgcca ttgtggtgag
    50221 cggatagaac cttgacgagc acgtccagtt cctttttgac gccatggttt gcgtccacca
    50281 cctgatactg cagagcggtt tttaacagcg tgtgttcctt gacgaaggct tgcgcgttgg
    50341 ctgatgatta catcaaacac aactgattca tttggttcga taccaaatac tgcatcgcta
    50401 agaacaactt ggccagcttc tttaccagtt tggtcaaata atgttacgtt tgccattgtg
    50461 actgatttcc cctttcttta ttatttacca gctttaactg ctgatttgat agtgataaga
    50521 gatttcttag cacctggtac gttacctttg ataaggataa cgttcttttc tggaacaact
    50581 tgtacaactt caaggttttg aattgttacg cggtcgccac ccacacgccc tgcaaggttt
    50641 ttacctttga atacgcggtt aggtgcaaca ggtcccatag aacctggacg acggtggtaa
    50701 cgagaaccgt gagccattgg tccacgtgat tgtccgtggc gtttgataac accttggaaa
    50761 cctttacctt tagaagtacc agttacgtca acaacgtctc cagctgcgaa tgtttcaact
    50821 gtaatttcag caccaacttc caagccttca acgtttttga attcacgaat gaagcgctta
    50881 ggagccgtgt tagctttcgc tacatgtcct ttagcaggtt tgttgctcaa tacttcgcgt
    50941 ttgtcatcga aaccaacttg gatagcgttg tatccgtctg tttcaacagt tttaacttga
    51001 agaacaacgt ttggagttgc ttcaataact gttacaggga tcaattcgcc agcttcagtg
    51061 aagatttgag tcattcccac ttttttccct aagattcctt ttgtcatgag aaaatagttc
    51121 cttttctata tttttattca aaaagttttt aacgagcgtt ttttatgctc aagtctaaga
    51181 tacaaagggc gtcaaactca acgtgaaaat aggaaactga cacagtgtct agcagacgcg
    51241 aggaagttta tctttttcac accgagttta gcccgtgttc aattagattg aaacaccagc
    51301 ctctgctctt ttatatttta gattaaagtt tgatttctac gtttacacca cttggaagat
    51361 ccaatttcat caaggcatca actgtttttt gagttgggtt aacgatatcg atcaaacgtt
    51421 tgtgtgtacg catttcaaat tgttcgcgag agtctttgta tttgtgagtc gcacgaatga
    51481 ttgtgtagag gctacgttca gttggaagtg ggattggacc cgcaacttgt gcacctgtac
    51541 gagtagctga ttctacgatt tttgcagccg ctgtgtcaag cgtacggtgt tcgtaagctt
    51601 tcaaacggat acggattttt ttgtttgcca tctttttctc cttttcgtct atttaagata
    51661 ataggctagc tccacaagaa aaccgacgcg gtgttgcgtg gcaatgcaac cgagcgtgtc
    51721 gcaacctctt gcatcaaaac taaggctgta atttacagca ccataataga ataacacaaa
    51781 tcccctgcga ttgcaaggga tttgttaggt tttttcgttt tttggggatg aaactgtttt
    51841 cctacttttt ctttctaaaa ttagggattt tcttctatat ttcttctata cataagttaa
    51901 gtcctcttga catcaaattt atcttctgat tggtccacta agatatccgc cttagactca
    51961 gtttctttat agtatcgcag atactgctcc cgtctcatct gatggctagc caatataaag
    52021 gatgcatcgc gatttctcac agtcgtatct ctagcaaggc gtcgctttaa ttcggtctcc
    52081 tcatccgtgt agaaacagat ggttttttca aagagttcct tgggtagaaa gccaacagac
    52141 atcccttcga caatcaaaat tggtttggct ccagacaaga cctcactagc cttccaaggt
    52201 tcttcaattg tcaagacatc catacccgcc tgcaaggcaa ggatatctct ctgcaaactc
    52261 tccagttcat gcgccactgg cagactggct gtcacctttt gattcggcgc gtccttgggt
    52321 actaccaaat agcgtccgga ggtgatatag ggatctgttt ctagcaaatt tactgtagta
    52381 gaatctaaag cttggtacaa ttcctgtgca aaggttgatt tacctgaagc tccatgaccg
    52441 tatattccca gtgtcctgac tttccccgtc tcgatctctg agactagttg gtctactaag
    52501 tcttttttct tcattctctc ttcacctgtt catagatttc ttttccgtca ttgagcttgt
    52561 cccaaatcac tacttgccca cttttgagcg atttttgcac atcgataatt cgttggttag
    52621 acgaacctcg aaactggagc ataagatttc tcttagttcg atcatatctt ccatcgacaa
    52681 gaatgtcaat cagtgacaag agttccagtt tatctggagt ttccaacatc atttcttccc
    52741 aagtgtagcc cgtccaggac cagatgtcct tgtctggcaa ttccttccga atccgcttaa
    52801 caagtggcaa gagaatccca gtattaagaa aaggctcccc tcctagtaag gttaacccct
    52861 gaacataagg ctgggcaaga tctgccataa tctgttcttc caactcagct gtatagggaa
    52921 tgcctgcatt gaaggaccag gtcgctacat tataacaccc ctcgcagtga aacatacagc
    52981 ctgctacata gagagagtta cgcacgcctt cgccatcaac aaagttaaag gccttgtagt
    53041 caatgatacg accttgacta agttcctcgc ttttccattc ttgtggtttt ggattattca
    53101 ttcgctacct ctatccaata acgctcgact ccattgcgag catcctcaaa tattccacca
    53161 tttgctagaa tgactgctct gctagcagga ttattcacgc tacaggtcac cagagctttc
    53221 ttgatgttct tttccttagc aacttgcaag ccctgacgga gagtctcttt tgcataaccc
    53281 ttgcctcttt cagatggacg gatggagtag ccaatgtggc cagcataatt cagtaacccc
    53341 tcattcagtc tcaatcgcag attcaaaaat cctagagcac aacctgattc accaaacaat
    53401 acgaactgta tcgatggaac gcgattttca ggtagccata ttcccatttc tttattacgg
    53461 ttaccttcta gccactcctc gtaagaaaaa tcctcaacat cccaaaatcc tccatcatgg
    53521 gctgattggc tcttttcaaa ctctgtcatc atatctaaaa cagctttctt atccgctaat
    53581 cttggtctgc gtaattccat ccttacctcc cactatgaga aaagcgagag ctggctctca
    53641 cttttatttt ttcttgttct tgtttaaaaa ttgttctctg aagctaagca agcgttcttt
    53701 cttaccagct ccacctttag agtgtttctc gtgataacgg gtcacttgtg cgcgcccctt
    53761 atcgtctagt tgatattttc ccatttcatt tctttctaat ttgttacttg atgcccagct
    53821 attttaatcg ttgaaccatt catatgtttg acacgcgcag cgatttcctt gtgacgcccg
    53881 ttgaccatcg gtcttgcttg aggattacct aggtagccac aagttcgctt caccacatct
    53941 actgttttag ggtcgctatt gccacagttt ggacaagcaa accctctctc agttggttca
    54001 aaatcccctt caaagtcaca cttgtagcaa cggtcaatcg gagtattggt gcctagatag
    54061 cctacacggt cataagcata atcccagaca gcttccaagg cctttggatt ttgctgaagg
    54121 actggatact cacaataatg gatgaaacca cctgacgcac ctgcttctgg atagactttc
    54181 tcaaagtcca atttttcaaa cggtgttgga tttttacgaa catcgtagtg gaaagagttg
    54241 gtgtagtatt ccttgtctgt gatatcagga atagagccaa acttgtctat atctagtcgg
    54301 cagaaacggt ctgtcagact ttcggatggt gttgagtaga tagagaaatg gtagccatat
    54361 tggtctgacc actcttctac acggcgtttc atatcgtgaa tgatgtctag cgtgaattcc
    54421 ttagcatctg gattactttc ccagctgtta ccaaagaaaa ctgtcgctac ttcatacaag
    54481 ccgatatagc ccagcgaaac ggtcgcacga caattcttaa agagctggtc aacactttct
    54541 tctttaccta gacgatggcc aaaagcaccg tactgataaa ggataggagc attcgctggt
    54601 gtcgcctctt tagtgcgttc gacacggtaa acaagagcat cttctgcgat attcattcgc
    54661 tcgttgaaga tttcccagaa cttattcata tcaccttcag actcaagagc aatacgaggc
    54721 agattaaccg tcacaacacc cagattcatg cgacctgaat tgacttctac accattttca
    54781 tccttccatc cttggaggaa agaacggcaa cccataggca ccttgaagga tcctgtcaag
    54841 tcaacaatct tatcgtaaga taaaacatct ggatacatcc gtttggttgc acactcaaga
    54901 gccaactgct tgatgtcgta gttgggagtt ccttcctcta aattaaggcc tcttttcaga
    54961 gtgaaaataa gtttaggaaa gatagccgta cggtgttctg aaccaagacc cttgatgcga
    55021 atgtttaaaa tagctttttg aatttctcgt tcaaaacgac tggttcccag accaaaacct
    55081 agcgaagtaa aaggtgtttg tccatttgaa gtgaagagag tattgatttc atactcaaga
    55141 gattgcatgg catcgtagat gtccttttgc gttttcttcc aagcgtaatc ttcccgtttt
    55201 tcaggcaata cccactcttc tgcatctttg agatgttttt gatagttctt ctctgcataa
    55261 ggcgccaaaa tttcatcgat acggtcagct gaacagccac cgtactggct agaagcaacg
    55321 ttggcaatga tttgagaaat ctgtgctgtc gcagtctgga tagacttggg actctctacc
    55381 tctgcatttc caatcttaaa accattttcc aacataccct taaaatcaat caaacagcag
    55441 ttggtcatag gggtataggg actgtagtcc aaatcgtgat agtggatatc ccccttttgg
    55501 tgggcattgg ctacgtgctt aggaagcatt tgcagtccga ttgatttccc aacaatccct
    55561 gctgtcaaat cacgctgagt gttaaagaca tcactgtctt tattagcatt ttcattgaca
    55621 actgtctggt ctttgttgag aagtttatga atactaaagt tgatatccgt cgcttttgag
    55681 cgctcaaaat ccctctgtgt ccgataagta atatactcct cagccagcgc atattctttg
    55741 gcttcaagga gttcatgttc tacgatattt tgaatttcgt aaatcttaat tccctgtgga
    55801 aagcgactat gaatttctgt aataattctc tcagtcagat tattgaggcg tttttcaacc
    55861 aggggtgtca catccataac cttgtcagct gccttgtgga gagccttgtc aatcttgtcc
    55921 acatcaaata caacacgtct cccatctcgt ttctcgacga agagagttgg caaaattgta
    55981 attttttctt ctagtgcaat catatgcatg ctcttttcta aaatgttctt tctaaaaagt
    56041 attataccac tctaaaaaga aaaatcaata tcttgtgtta aaaatcctaa aatttttaaa
    56101 aatacacaag atattgattt ggttatttaa ttttatagac tttaaactct gagtaatcag
    56161 tctttacttg ttggtaattt tcttttaaaa gtgtttccac ttcagaccag actaccacct
    56221 tgtcatttac cacaatcacc ttaggttgtt tttctttcaa gtcattaagt aacttattac
    56281 gattttcctc agttgctgta tagtgcaacg gggacaaaat cgccgatggc gacaagcgct
    56341 cactcttacg ataaagagtc gcagtatcat cccatgcata gatagcatct tccgtatttg
    56401 tctcctcttt taccaaatca gcaagacgat tacgttcttg ataaggtact ggataaagga
    56461 caaacttcat caaaaaagga acagacaaaa gatagacaaa cccaaaaact agcagatata
    56521 gatttccttt gaagaaacga ctccataggc taactggcgt ttctcttcgg cttcttctag
    56581 cactacgccc cctctgttga ctcttgatat tggttaccaa taaaagtagg aaaacaggga
    56641 agacgaccat caaataagaa gcgtgcagag gctcttgtga aaagaaaagt aaaatcaaac
    56701 ctaaaattac cacaagactt gcaggaaccg aaatgacgta tagtttaaag ggtttggatt
    56761 gaaagagacc taaaaagata caaaccacaa atcccaaacc aagggacaac aagccataaa
    56821 aaaacatatt ctctaaaatt ttagaagtaa aatcaaagcg aatactgtca ataggataac
    56881 gaataccact aatcgcatcc ccaaaacttc ctgttgcagc actatagtag gcagttggat
    56941 aaaagacaag tgaaaaacct aaagccactg caagaaactg ataaaaccca tgcgcaaagc
    57001 gtctatgccc aaggttaaag accaacaagc ctaaactcac tacagcaata aacaggagtg
    57061 atgataaggg agcaaagaaa aatccgcctg ccaaagctaa cccaatccgt acaaatccct
    57121 tatcatggct tggattgctt aggtaagccg caactaaact aaaggctgcg aataagaaag
    57181 gaagcgctaa aagagtcgca tagcctccac caaaagcaag acctgtaact agcatgtaaa
    57241 aaatagtcac cagatgtcca gcttggtctc cttgctctgt caaggtgtcc gctgatctaa
    57301 aaaggaaaaa tcctcctgct accaaggcta accactcaaa gatggcaaag aaaaatccgc
    57361 cctgactcac gtaagtcagc aaataataaa gcaaaccttg acttccataa tagtcgctgt
    57421 aaatcttccc tgtctgatga agcgcccaac ctgcataaaa atcctgcact tcttgtgcac
    57481 tcattaagtc gagtaatagc ggtactccta gagttatccc cgttacaagc gtactccata
    57541 gtaaaatttt caccaaagga agacgacttg attcacgatg atgcgattct tgctcgattt
    57601 ggtattctag aggttcacga ttctccttat gaacttcttc tactctacca tacacactca
    57661 tatcgtttct cctgttcaat ttatctgtct tagtatatca aaaagtccta ggattgtcag
    57721 cttgcgatgg ctgtttgagt gattttttgc atagtttcac aaaggtttca atttcccgaa
    57781 agcggtgtaa atgtgtctgt ttgaactcaa tcatatcatt cacttcttcc ttagttagca
    57841 tgaattcccc tcctctttct ttctattcgt aaaagacaag aaaaataggt cgggtttgta
    57901 tcctaagttc tagatttctt ataagatagg ggcgaatagc tgggcgattt cttttacaag
    57961 cttttgatac cagctcgttt tgatagaatg aggatagact tcctgagaat ctgcaaatat
    58021 atttcgaaaa tctcgggcga tttcccttat agaaggagtt ttatagagta agactgcatt
    58081 ttcataatgg tgtaccaagc tccggtagtc gagattgatt gtccccacca ccgcaaaatc
    58141 ttcgtctacc aacatctgct tactatgaat aaaacctgga ctatactcat aaatccgaac
    58201 accagcagaa agaaggtcgg gataagctcc tctcgtgact aactgaatga acttcttatc
    58261 tggtatgtaa ggggtgataa ttcgaacatc gacccctctc atagctgcat ttttgattgt
    58321 ctctgttaaa tcataatcta taatcaaata aggtgtcgta atatagaccg attccgttgc
    58381 ttgattgatt aaactctgat aaactttttt ccctacctgc gctcgaaaaa ttggcttggg
    58441 tccacttccg tatggaatgg ttaaaccgtc actcgggata gaatgatttt ctaaatgata
    58501 ttgatcaaaa tcactaattt ctcctcgatt aatgtaccaa gtggtcaaaa ataagcgtgt
    58561 cagggctttt actgctagtc cgtctaagcg aattccacta tccttccaat aaccaaatct
    58621 ctcgacgtgg ttaatgtact catctgccag attgacccca ccagtatagg ctatctgacc
    58681 atcaacaatc aatatttttc tatgatctct gttattataa gccactgtca aacgaggaat
    58741 aactttattg aatttatggg cctcgatgcc cagctgacga agtcgatgtg cataatctcc
    58801 tgttaaagta gccatacagc cgatatcatc atagagcatc ttaacctcta caccctgagc
    58861 taccttttgc tctagtatat ctagtatgcg attccacatc aaaccttctt ctataatgta
    58921 atattccaag aaaataaatt tctcagcctt tttaagatct tctaccatct ttttccacat
    58981 agcttctccg ttaggaaaaa atgtagaggc agtttgatca tagatgtcag cattggtatc
    59041 catactcaat aaggacttga tgactccata agctgccttg tcactttctt ttaatttctc
    59101 ttttagtagc tggctatttg cttcttggaa atgcatagag cctagcttct tcagttgttt
    59161 gatttctttt ttggacaatc gcctttcacc aaacatcaga tagagtaagg gaccaaatac
    59221 tggcacaaag gctactaaca accaggttac cttattctca ggagtcgtat tacggttgac
    59281 tatcgaaatg attgtgatga tactcagtag aatgaggaca gtaatccata aaatgggagc
    59341 catgcgtcct aaataaagaa agagaccaaa aacaagacaa agttcaagta gcataatcga
    59401 aagactaaag ccatacttgg acatcaataa ttgaaatttc ctatatttca tatcccctcc
    59461 ttgatagttt gaatgctaaa aacaagtaat tgatactagt ataacgcagg tattcggtag
    59521 aaggcaagta tttatgatta ttttctaggt aaaagtaaaa aggctgaaaa atccagccct
    59581 ttctacttgc atagtcttat ttagttaaaa cgatttggtc cgatgggtct ctatccatat
    59641 catctacgac gatttgatta ccgtttacag tgtaaatttt gacatcatcg ccaataatca
    59701 tgcgttgatt tgctgaatca aatgtaacct gtttgacttt ctgatccccg tcagattcga
    59761 gctcagtcca tgtaccagtc gttccagtta ccactaaagt gatacggtct ccgtcatcct
    59821 gaccagtata agtcccatca atatcggtcg gttgagcaac aggttggttt tgtactggag
    59881 ccgcttgatt ttggttacct cctgcatttg cagtattggt attctgttga gcaggttgtt
    59941 gagcaggctg ttggacctgc tgctgagcaa caggctgttg aacctgttga gctctttgtg
    60001 aacaagctac taagagacta aaacttgcta gagaagcaag agaaagtgtg aatcttttta
    60061 atttcatgat gaatttcctt tctgctacca atttagagaa attttctcta accagcaatt
    60121 cccctagtat aacaagttca aacaaggggg tcaatttatc tgctcacggt ccagcaggta
    60181 gccccgtact tctgagataa aatagagaga ccctgtaacg aacagcaagt cttgagcgtc
    60241 tgccctttct tcgaaactgc tgataaattc tcggcaagaa gaaactatat cgtaacctgt
    60301 cacatccctt tcgtccaaag ccccctgata gtcaaagccg gtcaccttga gttccacctg
    60361 aggcaatttt tcagtcaggt aacctaacat cccttgataa tccttccgtt tcaaggctcc
    60421 aaaaaggatt tgaggtcgat agccttcctg ctctttttct ttgataaact caaccaagcg
    60481 agtcaaggca gggaggttat gagcaccatc caagtaaatc tgtgggcgaa tacgctccaa
    60541 gcgaccagcc caatgggtct gttccaaggc ctttcttaca gcctgttcat caacagcttc
    60601 ctttccttct cccataaaaa gaagaaaagt ttgcaacgcc aaggctgcat tctcctgctg
    60661 atagactcct tctaagccta tgttcagctg tgaaaaattt agtaaagagc ttgaaaaatc
    60721 accattcagc attaaaaaat cttgacctgc ctgataaagg ttaacagcta aagattcggc
    60781 ttttttctga cagacaagcc tagcttctgg aggcaatttc gcaatcactg ccttcttacc
    60841 agccttgaaa ataccagctt tctgctctgc aattgcttct agactatcac ccaaggtttc
    60901 ttgatggtca agcccaatgg aggtgatgac agcaagctct ccagttacca cattggtcgt
    60961 gtcaagtaag ccaccaattc ccacttctaa taaaaccaaa tccacctctt gctccctaaa
    61021 ataaagaaaa gcaaccaagg tcagcatttc aaaaaaggac aactggtcag gagtttgcag
    61081 aagcgttttc tccatctcct tgacctgatc agccaaacgg ataaagtctg cgtctgctat
    61141 aggttgccca ttaatgcaga ttcggtcatt gatagagacg atatgagggg aggtaaaggt
    61201 cgcaactttt ttgccatgcc ccataaataa ttccctcata aaagcaatgg tagatccttt
    61261 cccattagtc cctgttacgt ggataatagg gtaagactgc tcaggatttc ctaacaaatc
    61321 caccgcttgc tgtattcggc ccaaacctga tcgaaaattc aaaccaatcc gactatgaag
    61381 ccattcttct acttcaaaca tacacatctc cttgacaaaa gtccaatcaa ttatcgcatt
    61441 aaagtatggt tactaataaa aacaaggcca ggattttcgt cccgacctct tacctggtta
    61501 gctaataact agccactatg aatgtgaata tgggctaaaa acatccacta gatgttccaa
    61561 ctcttcccca tttctgggag ttgggctgat acagtctccc agactgtatc actcctccat
    61621 aaagctgttg aagacttctt caatcatgtt ccattcgtct tctgagtctt ctgggattgg
    61681 ttgcaattcg ccttctgttc catcttcatt ttcaatgaat gagtaagctt ggatttcaac
    61741 ttgtccgtct tcgtcttctt ctgcgttaac tggtactaga agaacatagt ttttaccaaa
    61801 ttcttctttt ccatcaattg tcaaaaggat ttcaaacaag gtttcatttc cttgctcatc
    61861 tactagtgtg attagttcac gttcttcgtg gtcgtggtta tgatcgtgtg acatagcctc
    61921 tcctttatat taaaattttc tatctaaata attttgtaaa atcagctgag ctgctaactt
    61981 atcaatgact ttcttgcgct tattgcgact gatatctgct tgttcaatca acatgcgctc
    62041 agcagccact gttgtcaagc gttcatcctg atagtctaat ggtaaaccaa aaaactcttc
    62101 tagctttgct ccgtaggctt gactagcttc tacgcgcggt ccacttgtat tgttcatgtt
    62161 tttaggcaag cccactacaa atcgttccac cttgtaagta tcaaccaatt ccttaacgcg
    62221 gtcaaaacca aattggcctt gttcttcatt tatctggatg atttcaagcc cttgagctgt
    62281 aaaaccaagc ggatcgctaa tcgccacccc taccgttttt gaaccgacgt ccaatcccat
    62341 aattctcata ggttatagat cgactccttg tcctttgagg tagtagcgaa ccaattcctc
    62401 aacgatttca tcacgctcat acttacggat ttgatttcgt gcattattat aacgaggaac
    62461 gtaggcaggg tctccactca atacgtaacc tacgatttgg ttaattgggt tgtaaccctt
    62521 atcgttcaac gaagcataaa catctgtcaa agtttcgcta atttcttttt tattggaatc
    62581 gtccaattta aaacgtactg tttcttcagt aaatcccatt ctaacaccct ctttccttag
    62641 aatagtacca ttatagcata attccttacc ttctacaatt caggcagtct atttatttgg
    62701 attttctatt gttctgtcgc gccatttgcc aatctatctg aaatatattt gcttggttca
    62761 tttttcaaaa gattttccaa accaatattc ttcagatgtt ccaactggga agccttcttg
    62821 acatccagaa cttgaaaatc aaaactagtc gttgtttgaa gttccgttgc gctcaatagt
    62881 tttgtttcaa gtttgaaacc tgccaattta cgagcttcaa tgatagactt atccttctcc
    62941 tccgcttcaa gaagagcttt ttgagtttcc tccactccat gttggtcaat ataagtcttc
    63001 aactcatcag agactggacg tttttgttgg atagttagac tcaaaaaagt cttgtccttg
    63061 tatgtaatgg tttgtgtctg ctggctacca tcatctgact tgggcaagac caaagtctta
    63121 gttacaactg tattcttctc agcattttca ataactggca atgccgactg aagcgtatct
    63181 ttttctgttt ttgtagctgg tccagtttct tttttctgtc cgcaaccaac caggacaaaa
    63241 aggaaagcta gactaacaag aactattttt ttcatttctt tcttctttct ttttgaaatt
    63301 aaaatagaat aagactggga agtgctccca gccttgatgt ttatagagct gcacgcaaac
    63361 gtgcttctgc attttctaca ttacggacag agcgtggtag gaaggcacga atatcgtctt
    63421 ccttgtagcc aacttgcagg cgtttttcat ctacaaggat tgggctcttt aaaattctcg
    63481 gtgtttccat aatcagattg agaacttcat tgacactcaa atcttcaata tccactccaa
    63541 gggctttggc atagcgattt ttagacgaaa cgatgctggc tattccgtta tctgttttgg
    63601 ttagaatatc cagtaattct tctctcgtaa ttccttcttt accaaggttt tgttctttat
    63661 aacttaactg gtgggcattg agccaggttt ttgctttttt acagctagta caacttgaga
    63721 ctgtataaat tttaatcatg tacctacccc tttcgctaca tgttactatc agtttagtct
    63781 attataccat aaaaaacatc cgacttgcga cctattttta at
//
LOCUS       DAVBCW010000002        53965 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVBCW010000002 DAVBCW010000000
VERSION     DAVBCW010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 53965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53965
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.002"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(475..1503)
                     /gene="queA"
                     /locus_tag="VQ514_000076"
     CDS             complement(475..1503)
                     /gene="queA"
                     /locus_tag="VQ514_000076"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HET2498905.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIINMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDSMFIY"
     gene            1654..2361
                     /locus_tag="VQ514_000077"
     CDS             1654..2361
                     /locus_tag="VQ514_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HET2498906.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            2501..2677
                     /locus_tag="VQ514_000078"
     CDS             2501..2677
                     /locus_tag="VQ514_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HET2498907.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            2807..3742
                     /locus_tag="VQ514_000079"
     CDS             2807..3742
                     /locus_tag="VQ514_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HET2498908.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLSETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            3735..4523
                     /locus_tag="VQ514_000080"
     CDS             3735..4523
                     /locus_tag="VQ514_000080"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HET2498909.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGLRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            4524..4907
                     /locus_tag="VQ514_000081"
     CDS             4524..4907
                     /locus_tag="VQ514_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HET2498910.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            4923..5312
                     /locus_tag="VQ514_000082"
     CDS             4923..5312
                     /locus_tag="VQ514_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HET2498911.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            5396..6526
                     /locus_tag="VQ514_000083"
     CDS             5396..6526
                     /locus_tag="VQ514_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HET2498912.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            6531..7268
                     /locus_tag="VQ514_000084"
     CDS             6531..7268
                     /locus_tag="VQ514_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HET2498913.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEDYNLVWIIVEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHAVEPEIVAPYQSDFDKKLIRGPKYESLEESISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKA"
     gene            7279..8052
                     /locus_tag="VQ514_000085"
     CDS             7279..8052
                     /locus_tag="VQ514_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HET2498914.1"
                     /translation="MKYKGYLIDLDGTIYKGKDQIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKEKPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            8042..8659
                     /locus_tag="VQ514_000086"
     CDS             8042..8659
                     /locus_tag="VQ514_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HET2498915.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLALVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYESFFGYLCLKSRRK"
     gene            8869..9270
                     /locus_tag="VQ514_000087"
     CDS             8869..9270
                     /locus_tag="VQ514_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HET2498916.1"
                     /translation="MITLFLSPSCTSCRKAKTWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            9270..9548
                     /locus_tag="VQ514_000088"
     CDS             9270..9548
                     /locus_tag="VQ514_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HET2498917.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGKGKISLEK"
     gene            9538..10311
                     /locus_tag="VQ514_000089"
     CDS             9538..10311
                     /locus_tag="VQ514_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET2498918.1"
                     /translation="MKSKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLASFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            10312..11616
                     /locus_tag="VQ514_000090"
     CDS             10312..11616
                     /locus_tag="VQ514_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HET2498919.1"
                     /translation="MKFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQIFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEEFVKYAAGET
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            12190..13068
                     /gene="pstS"
                     /locus_tag="VQ514_000091"
     CDS             12190..13068
                     /gene="pstS"
                     /locus_tag="VQ514_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HET2498920.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            13068..13985
                     /gene="pstC"
                     /locus_tag="VQ514_000092"
     CDS             13068..13985
                     /gene="pstC"
                     /locus_tag="VQ514_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET2498921.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            13975..14859
                     /gene="pstA"
                     /locus_tag="VQ514_000093"
     CDS             13975..14859
                     /gene="pstA"
                     /locus_tag="VQ514_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET2498922.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            14870..15673
                     /gene="pstB"
                     /locus_tag="VQ514_000094"
     CDS             14870..15673
                     /gene="pstB"
                     /locus_tag="VQ514_000094"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET2498923.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            15686..16444
                     /gene="pstB"
                     /locus_tag="VQ514_000095"
     CDS             15686..16444
                     /gene="pstB"
                     /locus_tag="VQ514_000095"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET2498924.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            16456..17109
                     /gene="phoU"
                     /locus_tag="VQ514_000096"
     CDS             16456..17109
                     /gene="phoU"
                     /locus_tag="VQ514_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET2498925.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            17285..18082
                     /locus_tag="VQ514_000097"
     CDS             17285..18082
                     /locus_tag="VQ514_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET2498926.1"
                     /translation="MFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVVATSPDY
                     APFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEILSMSFDNVLTSLQTGKADLAVA
                     GISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGTVPESMV
                     KEQLPKAQLTSLTNMGEAVNELQAGKIDAVHMDEPVALSYAAKNAGLAVATVSLKMKD
                     GDANAVALRKNSDDLKEVVDKVIQKLKDEGTYQSYLEKAASLTEVEE"
     gene            complement(18102..19373)
                     /locus_tag="VQ514_000098"
     CDS             complement(18102..19373)
                     /locus_tag="VQ514_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET2498927.1"
                     /translation="MKKQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEEHVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQLGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            19515..20246
                     /gene="budA"
                     /locus_tag="VQ514_000099"
     CDS             19515..20246
                     /gene="budA"
                     /locus_tag="VQ514_000099"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HET2498928.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFLHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            20256..21008
                     /locus_tag="VQ514_000100"
     CDS             20256..21008
                     /locus_tag="VQ514_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HET2498929.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWFEKKR
                     SLEKADALAYGLGHGGLELIFLGLTSLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLTAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            21120..22070
                     /gene="murB"
                     /locus_tag="VQ514_000101"
     CDS             21120..22070
                     /gene="murB"
                     /locus_tag="VQ514_000101"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HET2498930.1"
                     /translation="MSVREKMLEILEGIDIRFQEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARLVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESLSVAKMYAGGFTPCKR"
     gene            22129..23286
                     /locus_tag="VQ514_000102"
     CDS             22129..23286
                     /locus_tag="VQ514_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2498931.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFESEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            23267..24073
                     /locus_tag="VQ514_000103"
     CDS             23267..24073
                     /locus_tag="VQ514_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2498932.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            24070..24843
                     /locus_tag="VQ514_000104"
     CDS             24070..24843
                     /locus_tag="VQ514_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2498933.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLAPSIITGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            24840..25910
                     /locus_tag="VQ514_000105"
     CDS             24840..25910
                     /locus_tag="VQ514_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET2498934.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQDS
                     AYAFINFMLKPENALQNAEYVGYSTPNIPAKELLPEETKEDKAFYPDVETMKHLEIYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            26163..26402
                     /locus_tag="VQ514_000106"
     CDS             26163..26402
                     /locus_tag="VQ514_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2498935.1"
                     /translation="MKMSTFFNKSFWPTFMIVNQTAILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILEK"
     gene            26677..27162
                     /locus_tag="VQ514_000107"
     CDS             26677..27162
                     /locus_tag="VQ514_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HET2498936.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSQISKEILTLLPRFEIQLLDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            27184..29802
                     /gene="alaS"
                     /locus_tag="VQ514_000108"
     CDS             27184..29802
                     /gene="alaS"
                     /locus_tag="VQ514_000108"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HET2498937.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLISPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGS
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNTSQLSSKLVAIVADNAEVGAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKIVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDAKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQAKIQELLDAVAGKL"
     gene            complement(29892..31346)
                     /locus_tag="VQ514_000109"
     CDS             complement(29892..31346)
                     /locus_tag="VQ514_000109"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HET2498938.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKEQGIQPMADVVL
                     NHKAAADNREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNGFHWHWYHFT
                     GTDYDAKRSKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            31525..32274
                     /locus_tag="VQ514_000110"
     CDS             31525..32274
                     /locus_tag="VQ514_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2498939.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLTSSDLETSLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSAPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGSVEN
                     LRKDHPDQFLESIYLSLAGRKEEVADASQGH"
     gene            32255..33889
                     /locus_tag="VQ514_000111"
                     /pseudo
     CDS             32255..33889
                     /locus_tag="VQ514_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            34574..35737
                     /locus_tag="VQ514_000112"
     CDS             34574..35737
                     /locus_tag="VQ514_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HET2498940.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVAFNQAFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGLDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDERRFIVMDVFEYF
                     KYAKRKNLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            35734..36411
                     /locus_tag="VQ514_000113"
     CDS             35734..36411
                     /locus_tag="VQ514_000113"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HET2498941.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            36401..37255
                     /locus_tag="VQ514_000114"
     CDS             36401..37255
                     /locus_tag="VQ514_000114"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HET2498942.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLVETVANIRRYQMFGINLSMPYKEQVIPYLDELSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKKMPTEEIW
                     QSLTEKYQ"
     gene            37274..38341
                     /locus_tag="VQ514_000115"
     CDS             37274..38341
                     /locus_tag="VQ514_000115"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HET2498943.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVENILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWKVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSATIHPVSLEEMKDYLVK"
     gene            38351..39517
                     /gene="aroC"
                     /locus_tag="VQ514_000116"
     CDS             38351..39517
                     /gene="aroC"
                     /locus_tag="VQ514_000116"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HET2498944.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            39527..40630
                     /locus_tag="VQ514_000117"
     CDS             39527..40630
                     /locus_tag="VQ514_000117"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HET2498945.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLEVIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            40641..40979
                     /locus_tag="VQ514_000118"
     CDS             40641..40979
                     /locus_tag="VQ514_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HET2498946.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            41071..42354
                     /gene="aroA"
                     /locus_tag="VQ514_000119"
     CDS             41071..42354
                     /gene="aroA"
                     /locus_tag="VQ514_000119"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HET2498947.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKEYTRNHTEDMLQQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIAPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSATLIVESSDLKGTEIGGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            42347..42823
                     /locus_tag="VQ514_000120"
     CDS             42347..42823
                     /locus_tag="VQ514_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HET2498948.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRHVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     LRIAADKDNQRPLFLNNSKEELVAIFQERQVWYEEVASRVLDVTKLSPEEIIEELR"
     gene            42820..43668
                     /gene="pheA"
                     /locus_tag="VQ514_000121"
     CDS             42820..43668
                     /gene="pheA"
                     /locus_tag="VQ514_000121"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HET2498949.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYIDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            43665..44939
                     /gene="psr"
                     /locus_tag="VQ514_000122"
     CDS             43665..44939
                     /gene="psr"
                     /locus_tag="VQ514_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HET2498950.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            45218..46063
                     /locus_tag="VQ514_000123"
     CDS             45218..46063
                     /locus_tag="VQ514_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HET2498951.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKDFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLTLDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            46053..47156
                     /locus_tag="VQ514_000124"
     CDS             46053..47156
                     /locus_tag="VQ514_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET2498952.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDLEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLTRRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYASKLQIIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            47158..48144
                     /locus_tag="VQ514_000125"
     CDS             47158..48144
                     /locus_tag="VQ514_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2498953.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQIYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            48160..49593
                     /locus_tag="VQ514_000126"
     CDS             48160..49593
                     /locus_tag="VQ514_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HET2498954.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFIAKMLTKEN"
     gene            49622..50656
                     /locus_tag="VQ514_000127"
     CDS             49622..50656
                     /locus_tag="VQ514_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HET2498955.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPKESAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            50856..51593
                     /gene="mecA"
                     /locus_tag="VQ514_000128"
     CDS             50856..51593
                     /gene="mecA"
                     /locus_tag="VQ514_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HET2498956.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            51744..53030
                     /locus_tag="VQ514_000129"
     CDS             51744..53030
                     /locus_tag="VQ514_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HET2498957.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILALDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            53032..53901
                     /locus_tag="VQ514_000130"
     CDS             53032..53901
                     /locus_tag="VQ514_000130"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HET2498958.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTKGVRVEAK"
BASE COUNT        15473 a        10136 c        12314 g        16042 t
ORIGIN      
        1 aaactagtcg caggttgctc aaagtactgc tttgaggttg tagatagaac tgacgaagtc
       61 agtcacatat ataatccaag gtgacgttga cgcagtttga agagattttt gaagagtata
      121 agtctttgat tttgagacta aggagagtca gggtcaaagt agtgaatgat tctgaaataa
      181 ctcataatag tctcttgaga ggacagataa gagtaacaaa tatggtgcta ctcatgtgtc
      241 ttgcattttg atttacaaac agcgaagaaa atataggaag ctgaaataaa atatagatga
      301 atcaatttgg agagtcaacg caatctatgg caatattttc gaagctaggg ggaagcattt
      361 taatttcaat gtactaggcg tcttctacag ctagctagag tttcaattgt agatgttctt
      421 tgaaataata tcttagcgat ttattggtat tagaagattt agagaaattc tttctcaata
      481 aataaacatg gagtcaccaa aactgaagaa gcggtagtgt tcttggatgg catggtggta
      541 ggcatctaag actaattcac ggcctgcaaa ggcagaaacc aacatgacca gagttgattt
      601 tggcagatgg aagttggttg agaaggcatc cacgaccttc cactcatacc caggtttgat
      661 aaagatattg gtccaaccag aatctgcttg gatttgccca tcaaacttgg aaccaatagt
      721 ttccaaggtg cggatagaag tggttccgac agcgatgacg cgaccaccat tttctttgac
      781 agagcgaagg gtggcagcag cttcctcaga aagttgatag aactctgaat gcatttcgtg
      841 ttcgtccaga ttatccacag aaacaggtct aaaggttccg agtccgacat ggagagtcag
      901 atagactaga tgaacaccct tagcttggat ttctgccagc agttctttgg tgaagtgaag
      961 accagcagtc ggtgctgcag cagagccgct ttccttggcg tagacggttt gataacgttc
     1021 acggtcatct aatttttcgt ggatataagg tggcagaggc atttctccca gactttccaa
     1081 gacttctagg aaaattcctt ggtattcaaa gcggacaatg cgtcccccgt gggtcaattc
     1141 ttctgtaacg acagcgctga ggcggccatc accaaagctg atacgagtac cgaccttgag
     1201 gcgtttggca ggtttagcca gaacttccca ctcgtctcca ctagtgttct taaggaggag
     1261 aagttccaca tgacctcctg tctccacttt ttgaccatag aggcgggcag ggagaactcg
     1321 ggtgtcgttc atgacaaggg catcaccagg ttccagcata ttaataatag agtggaagtg
     1381 tttatcttgc atttctcctg tctcacggtt gacgatgagg agtttggagg catcacgttt
     1441 ttcaaggggc gtttgggcaa tcaattcctc tggcaagtgg aaatcaaaat cagctgtatt
     1501 catatatctg ttcattcttt ctaagttcta atcctatcca ttataacacg tttcaagttt
     1561 ggacgctctt tttttagaaa ttaaatcgtt ttcacttgac aaaaattggt ctataccata
     1621 taataaatat agacagaaat ggaggatgaa aagatgaaag ttattaaagt tgaaaaccaa
     1681 gttcaaggtg gaaaagttgc ttttgagatt ttgaaggaaa aattggctaa tggagctcaa
     1741 actctaggac ttgcgacagg aagtagccca cttgaatttt acaaggaaat tgttgagagt
     1801 gaccttgatt tttcaaatct aaccagtgtc aaccttgatg agtatgtggg ccttgatggg
     1861 gacaatccac agtcttatcg ttacttcatg caagaaaact tgttcaacca aaaaccattt
     1921 aaagaaagtt tcttgcctcg tggtgttaag gacaatgctg aagctgaagt tgaacgctac
     1981 aaccaaattt tggctgacca tccagttgac ctccaaatct tgggaatcgg tcgcaatggg
     2041 catatcggct ttaatgagcc tggtactcca tttgacagtc aaacgcatct agtagaactt
     2101 gaccagtcta ctatcgaagc caatgcacgc ttctttgcca agattgaaga cgtcccaacc
     2161 caagctattt caatggggat taaaaacatt ttggatgcca agtctattat tctttttgct
     2221 tacggtgagt cgaaagcaga agccattgcc ggaacagtat ctggcccagt gactgagaac
     2281 ctacctgcaa gtagcctcca aaatcaccct gatgtaacca tcatcgcaga tgctgaagcg
     2341 cttagcttac tcgaaaaata aaaaagcaat gttctattga acgctttcaa ctcttgtata
     2401 aatggaagaa aaaatcaaaa ttaaacagca tttttgcttg acaattattc cttttacgtg
     2461 tagaatgaaa tagatatcga acttgaaggg agtgaaaaaa atgtctaaaa cagtagtacg
     2521 taagaatgaa tctcttgacg acgcacttcg ccgtttcaaa cgtgcggtta ctaaagctgg
     2581 tactcttcaa gaaacacgca aacgtgaatt ctatgaaaaa ccttctgtaa aacgtaaacg
     2641 taaatcagaa gtagctcgta aacgtaaaaa attctaattg gaatgaaagg ctagagaaat
     2701 ctagtccttt ttcttttaaa taaatactcc aaagcctgca aaaatctgaa acttcctcct
     2761 acaatttgat ataatagaga gaagaactca tttgaaggag gaaatgatgt cggttttagt
     2821 aaaagaagtg attgaaaagc ttagactaga tattgtctat ggtgaaccag agttgcttga
     2881 aaaggaaatc aatacagcgg atattacgcg acctggtctt gaaatgacag gctattttga
     2941 ctactacaca ccagagcgga ttcaactttt ggggatgaag gagtggtctt atctgatcag
     3001 catgccttcc aacagccgtt atgaagtttt gaaaaaaatg tttctatctg agacaccagc
     3061 agtcattgtt gcccgtggtt tggtggttcc agaggagatg ttaaaggctg ctagagaatg
     3121 taagattgct attttaacca gccgtgcagc taccagtcgt ttatctggag agttatctag
     3181 ctatctggat tctcgtttgg cagaacgtac cagtgtgcac ggtgtcttga tggatattta
     3241 tgggatgggc gtcttgattc agggagatag tggaattggt aagagcgaga caggtcttga
     3301 gcttgtcaaa cgtggtcacc gtttggtagc agatgaccgt gtcgatatct ttgccaagga
     3361 tgagattact ctctggggtg aaccagctga aattttgaaa cacttgattg aaattcgtgg
     3421 ggttggtatt atcgatgtta tgagtctcta cggtgcgagt gctgtcaagg attcttcaca
     3481 ggttcagctt gctgtctatt tggaaaatta cgatacgcat aagacctttg atcgtcttgg
     3541 aaacaatgca gaggaacttg aagtttctgg cgtagccatt cctcgtattc gtattccagt
     3601 taaaacaggt cgtaatatct ctgttgtgat tgaggcagct gccatgaatt atcgtgccaa
     3661 ggaaatgggc tttgatgcga cccgtttgtt cgacgaacga ctgacaagtc tcatcgctcg
     3721 aaatgaggtg caaaatgctt gatccaattg ctattcaact aggaccccta gccattcgtt
     3781 ggtatgcctt atgtattgtg acaggcttga ttcttgcggt ttatttgacc atgaaagaag
     3841 cacctagaaa gaagatcata ccagacgata ttttagattt tatcttagta gcctttccct
     3901 tggctatttt aggagctcgt ctctactatg ttattttccg atttgattac tatagtcaga
     3961 atttaggaga gatttttgcc atttggaatg gtggtttggc catttacggt ggtttgataa
     4021 ctggggctct tgtgctctat atctttgctg accgtaaact catcaatact tgggattttc
     4081 tagatattgc ggcgcctagc gttatgattg ctcaaagttt ggggcgttgg ggtaatttct
     4141 ttaaccaaga agcttatggt gcaacagtgg ataatctgga ttatctacct ggctttatcc
     4201 gtgaccagat gtatattgag gggagctacc gtcaaccgac tttcctttat gagtctctat
     4261 ggaatctgct tggctttgcc ttgattctga tttttagacg gaaatggaag agtctcagac
     4321 gaggtcatat cacggccttt tacttgattt ggtatggttt cggtcgtatg gtcatcgaag
     4381 gtatgcgaac agatagtctc atgttcttcg gccttcgagt gtcccaatgg ctgtcagttg
     4441 tccttatcgg tctcggtata atgatcgtta tttatcaaaa tcgaaagaag gccccttact
     4501 atattacaga ggaggaaaac taaatgttag aagttgcata tattcttgtt gccctagctt
     4561 tgattgtctt tttggtctat ctgatcatta ctgtacaaaa gcttggtcgt gtcatcgatg
     4621 aaacagaaaa gacgattaaa accttgactt cagatgtgga tgtgaccttg catcacacca
     4681 atgagttgtt ggctaaggtc aatgtcttgg cagatgatat caatgtcaag gtggctacga
     4741 ttgatccact cttcagtgct gttgcagatt tatctctatc tgtttcagac ctcaatgacc
     4801 atgcgcgtgt cttgagcaag aaagcttcat cagctggttc aaaaacactc aagactggtg
     4861 caagtctgtc agctcttcgt cttgcaagta aatttttcaa aaaataaaaa aggagaatcc
     4921 ttatgggtaa attatcctca atccttttag gaaccgtttc aggtgcagct cttgccttgt
     4981 ttttaacaag tgataagggc aaacaagttt gcagtcaggc tcaagatttt ctagatgatt
     5041 tgagagaaga tccggagtat gccaaggagc aagtctgtga aaaactgaca gaagttaagg
     5101 agcaggctac agattttgtt ctgaaaacaa aagaacaggt tgagtcaggt gaaatcactg
     5161 tggacagtat acttgctcaa gctaaatcct atgcttttca agcgacagaa gcatcaaaaa
     5221 atcaattaaa taatctcaag gagcaatggc aagaaaaagc cgaagctctt gatgactcag
     5281 aagagattgt gattgatata acagaagaat aaaccatcac catctccgga cggactatgt
     5341 atctggggat ggtgattttt atctggaatc tagtctttgt ggtataataa ttactatgca
     5401 gaaaaaacca acgtcagcct atgtgcacat cccattttgt acccagattt gttattattg
     5461 tgatttttca aaggtcttca tcaaaaatca gccagtcgac agctatttag agcatctgct
     5521 ggaagagttt cgttcttatg atattgaaaa gttgtcaacc ctttatatcg gtggtggaac
     5581 accgacagcc ctgtcggctc cgcaactgga ggttttactg aatggcttga ctaaaaactt
     5641 ggacttgtct gtcttagaag agttgaccat tgaagccaat ccaggcgatt tggatgcgga
     5701 taagatagct gttttgaaaa attcggctgt caatcgtgtt tcgctaggtg tccagacctt
     5761 tgatgataag atgttgaaaa agattgggcg cagtcatttg gagaaggata tttatgaaaa
     5821 tatcgatcgc ctgaaactgg ctggttttga caacatctcc attgatttga tttatgctct
     5881 gcctggtcag accatggagc aagtaaagga aaatgtggct aaagccattg gactggatat
     5941 tccccacatg agtttgtata gtttgatttt agaaaaccat acggtcttta tgaatcggat
     6001 gcgacgtggg aaattgcctc tgcctaagga ggaactagaa gcggagatgt ttgagtacat
     6061 cattgcagag ctggagcgag cgggttttga gcattatgag atttctaatt tctccaaacc
     6121 cggctttgaa agtcgtcaca atctcatgta ctgggacaat gctgaatact atggtattgg
     6181 tgcaggggca tctggttatg tcaatggagt acgctataaa aatcatggtc ccattcgtca
     6241 ttatctcagt gcggttgagg aaggcaatgc ttgtattaca gaagatcacc tgagtcaaaa
     6301 ggagcaaatg gaagaagaaa tgttcttggg actccgcaag aaatccgggg tttccatggc
     6361 gcgatttgag gaaaaatttg gacagtcttt tgctggactt tatggagaaa ttgtcagaga
     6421 tttggttcaa caaggcctca tgcagattga gggtgaccac gttcgcatga caaagagagg
     6481 tctctttttg ggagatactg tagcggaacg atttattttg gagtaggatc atgggcttaa
     6541 cttatcaaat gaaaatgaaa attccttttg atatggctga tatgaacggt catatcaaac
     6601 ttccagatgt aattttgctg tcactgcaag tttcagggat gcagtcgatt gaactgggag
     6661 ttagtgataa ggccattttg gaagactata atctggtctg gattatcgta gaatatgata
     6721 ttgaggtggt tcgtttgcct cgttttgcgg aagaaattac catcgaaacg gaagccttga
     6781 gctacaatcg acttttttgc taccgtcgct ttactattta taatgaagcg gggcaggagc
     6841 tcatccatat gatggcgacc tttgttctca tggaccgcga cagtcgaaaa gtccatgctg
     6901 ttgaacctga gattgtggct ccttaccagt ctgattttga taaaaaactt atccgtggac
     6961 cgaagtatga gtccttggaa gagtcgatca gcaaggatta ccatgttcgt ttttacgact
     7021 tggatatgaa tggtcatgtc aataacagta aatacttaga ctggattttt gaggtcatgg
     7081 gagcggattt tttgacccac tatattccca agaaaatcaa cctcaagtat gtcaaggaag
     7141 ttcgaccagg tggggtgatt acatcggctg ttgaacggac tggactggaa agcaagcatg
     7201 aaattacaag tgatggggtt atcaatgccc aagcaatcat tacttggcaa gaaataaaaa
     7261 aggcttaggg agaaatagat gaaatataaa ggctatttaa ttgatttaga cggaaccatt
     7321 tataagggga aagaccaaat ccctgctgga gagacttttg ttcatgaatt gcaaaagcgg
     7381 gacattccct atctctttgt aaccaacaat acaacccgca ctccagagag tgttaaggag
     7441 atgttggctc agaattttaa tatcgatacg cccctatcga ctgtctacac agcgactttg
     7501 gcaaccatcg actatatgaa tgatttgggg cttgaaaaga ccgtctatgt tgtcggagaa
     7561 gcaggactca aggaagccat caaggcggct ggttatgtgg aagacaagga aaaacctgcc
     7621 tacgtggtag ttggtctgga ttggcaagtc gactatgaaa aatttgccac agcaactctt
     7681 gctattcaaa agggtgccca ttttatcgga accaatcctg acctcaacat cccgacggaa
     7741 cgtggtcttt tgccaggtgc tggctcactg attacgctgc ttgaagtagc gacacgagtg
     7801 aagccggttt atatcggaaa acccaatgcc atcattatgg acaaggcagt tgagcactta
     7861 ggtttggaac gtgaagagtt aatcatggtt ggggacaatt atttgactga tatccgggct
     7921 gggattgaca atggcattcc aacgctcttg gtgacgacag gttttaccaa ggcagaagaa
     7981 gtagctggcc tgccaatcgc accgacacat gtggtttcta gccttgcgga gtgggatttt
     8041 gatgaaaact aaattgacct tttggggctc tatgctcttt ctcctctccc tctcaatcct
     8101 tctgaccatt tatctggctt ggattttcta tcctatggag attcagtggc taaacttaac
     8161 gaatcgagtc tatctaaaac cagaaaccat tcaatacaat tttcatatct tgatgaatta
     8221 tctgaccaat ccttttagtc aggtcttaca gatgcctgat tttcgttcgt cagcagctgg
     8281 tttgcaccat ttcgcagtgg tcaagaatct ctttcatttg gttcagctag tagctctagt
     8341 gacactgcca agtttctatg tctttgtcaa taggattgtg aaaaaggact ttttgtctct
     8401 ttatcgaaaa agtctcctgg ctctagtagt cttacctgtg atgattggac ttgggggagt
     8461 tttgattggt tttgaccaat tctttactct tttccatcaa attctctttg tgggagatga
     8521 tacctggctt tttgatccag ccaaggatcc tgttattatg attttgccag agaccttctt
     8581 tcttcatgcc ttcctcctct tttttgccct ctatgaaagc ttctttggct atctgtgtct
     8641 gaaaagtcgt aggaagtgaa aggaaagatg tttttaattt acatatataa atatatatat
     8701 cgtttaaaac catcgttaag agtgaattaa ccaaatccaa ttgtgtcaat cgttcgcaaa
     8761 cagcctattt ttcctgaaaa aataggcttt ttttctaaaa atccctagtc aagcgcctta
     8821 tttttttgta taatagaagt agcaaagtat agataagaag agaaaagcat gattacacta
     8881 tttctatcac cgagctgtac atcatgtcgt aaggctaaga cctggttaga aaaacataag
     8941 gttccctttg tggaacacaa tattatgacc agtcctttaa caagaaaaga attgcaacac
     9001 atcctttcct tgaccgaaaa tggtactgat gacatcattt caactcgttc aaaaattttt
     9061 caaaaattga atattgatgt agagagtatt tcggtatcgg aattgcttca tttgattgag
     9121 cagtatccta gtcttttgcg tcgtccaatt attattgatg ccaagcgtat gcaaatcggt
     9181 tttaatgaag atgagattcg tgcttttctc cctcgtagtt atcgtaagca agaactaaaa
     9241 gaagcaagaa tgagagctgg tattagttaa tgaacaaaca gtatagttac ccactagatt
     9301 tgtcgtggag cactgaagaa cttgcttcag tgctttcttt ttttaatgat gttgaagctg
     9361 cctatgaagg caaagtagag gctaaaaagt tactggactc ctataaggga ttcaaagcgg
     9421 tcgtgccaag caagagcgaa gagaaacgtt tgggacgaga atttgaaacg gttagtggct
     9481 attcacttta ccgagcagtt caggctgcca aagaaaaagg gaaaggaaag atttctcttg
     9541 aaaagtaaat ttgaatttgc taaagagctt gttaagaaag caggtcagta cattcttgac
     9601 catatgcagg aagacttgcg tgttgaaacc aagtcctctc caacagattt ggtgaccaga
     9661 ctggacaaag aagttcagga actcttggtt ggtgagattt tgtcccgtta tcctgaggat
     9721 aagatttgtg ctgaagaagg ttgtctgcga gcctcggttc aagagggcaa ggtttgggtc
     9781 attgatccca ttgatggtac caataatttt gtagcccagc aggaagattt tgctgttatg
     9841 atggcttatt ttgaaaatgg tcagggacag tttggtctga tttatgatgt ggtcaaaggg
     9901 gattgttacc acggtggtgg ggaatttcca gtttgtctaa atgataggcc cctagcttct
     9961 tttaaaacta aaccacttgg agattttctc attgcaggga atagtggtat gctggaaacc
    10021 aatgaatggg ggctggctga tttgagccga gcggttcttg gtgttcgtgt ctatggaagt
    10081 gcggccatta gttttgccaa gattttgtca gggcgtttgt tgacctatct cacctatctg
    10141 caaccatggg attatgccgc agctagtatt ttaggggaaa gtctgggcta tcgggttgtg
    10201 accctttttg gtgaagctcc tgattttcaa accagacagc cggtcatgat ggtgcctctt
    10261 gagatgcagg aggaaattca gtcctatatt tacgaaagga aaagaactta aatgaaattt
    10321 ccagaaggat ttgttgaaaa atataaagaa atactaggag atgaggcaag agattttctt
    10381 gcctcttttg aggaggaagc ggtttcggcc tttcgggtca atcccttaaa agaagagcaa
    10441 ctttcctttt ctgatgccat tactcaaacc ccttggggcc actatgggaa ggtttcaggg
    10501 aaatcgcctg agcatgctac aggtttagtt tattcgcaag aacctgctgc tcaaatggtg
    10561 gctcaagtag cccaacccag tcctggtatg aaggtcttgg acttggctgc tgcaccgggg
    10621 ggcaaatcaa ctcaactggc agcctatcta gcaggggagg ggctacttgt ttccaatgaa
    10681 atttcaagca aacgggctaa gattttggtt gaaaacatgg agcgttttgg agcgacaaat
    10741 gtcgtggtga ccaatgaatc tgccgaccgc ttggtcaagg tctttaaagg ctattttgac
    10801 ttaatcgttc ttgatgcccc ttgctctggt gaaggaatgt ttcgtaagca acctgatgct
    10861 atggactatt ggagcttaga ttatccgagt caatgtgcta gcttgcaaag agaaattctg
    10921 gaggatgcag tgaccatgtt agctgaaggt ggtcgtctgg tttattcgac ctgtacctgg
    10981 gcacccgagg aaaacgaaga gattgtcaat tggttgctgg aagagtatga ttttgacttg
    11041 ttgccagtag agcatatcaa tggaatggta gctggtattg acctgccaga aacggctcgg
    11101 atgtaccctc atcagtttaa gggagagggt cagtttgttg cccatctaca gtttaagggt
    11161 aacaatccag ctcctaaatt taaggcaagt aagagtaacc tcagccgtga acaagttgcc
    11221 ttgtggcagg aatttgccca aaaccatttg aaggtcaatc taccgggtat cttgcagatt
    11281 tttggagacc agctttatct cttgccagaa cttttgccag atttagggaa gctcaagatt
    11341 gctcgcaatg gactgcatct gggtactttt aagaagaaac gctttgagcc cagtttcgct
    11401 cttggtttag ccttgaaacc gagtcaggtc gaacagtcgg ttgaaattgg ccaagaagag
    11461 tttgtcaagt atgcggctgg agaaaccgtt cagctggcag aaagtctgcc aaatggttgg
    11521 taccaagttt tggttaaagg caatggcttg ggctttgcaa aggttactgg aaatgttttg
    11581 aaaaattatt ttccaaaagg cctcagattc aaatgaaaat gctagtcaaa tatcttattc
    11641 tatgttatag tgaaagtatg gattttttta gtaggatgat tcttgatatc ttgattgcca
    11701 tcacatgtag ttttactact cgatgaaaat caaagagcaa actaggaagc tagccataga
    11761 tagctcaaaa cactgctttg aagagatttt caaagagtac aagaagttta gttattagcg
    11821 ttcttaccgc ttgtaaacta gatttctcat aaaatagaat cttttccttt tagttgtaaa
    11881 ctagtctggg agagtagaga gatttgagat acctttctag cttttggatt atcatctaag
    11941 gagagtaatt tcccttgcat taaaaagggg aaaaagagac acgaaatgac tataatgggt
    12001 gacaatgggg gaagggatag acaagagatt ttatccacat atgaaaaaag gaggttagga
    12061 aagagtaata tagcctatat tatataaata atcaattgcg cagaaatttg gtaagaattc
    12121 atgcgtcaac tcataaagaa ctacttaaaa aattcacagt attcataatt attttcgagg
    12181 agaaaaacag tgaaaaaaag aaaaaagctt gctctgtctc ttatcgcttt ttggctgacg
    12241 gcttgtttag taggctgtgc tagctggatt gatcgtggag aatccataac ggctgttggc
    12301 tcaactgcct tgcaaccctt ggttgaagta gcggcagatg aatttggcac gatccatgtt
    12361 ggaaaaacgg tcaatgtcca agggggaggt tctggtacag gcttgtccca ggttcagtct
    12421 ggggcagttg atataggaaa ctcagatgta tttgctgagg aaaaagacgg aattgatgct
    12481 tctgctcttg ttgaccacaa ggtcgcggta gctggcttgg ctctgattgt caataaggag
    12541 gttgatgttg ataacctaac gacagagcaa cttcgtcaaa tcttcatagg tgaggtaacc
    12601 aattggaaag aggttggtgg taaggactta cccatctctg ttatcaatcg ggcagccggc
    12661 tctggctctc gtgctacctt tgatactgtc attatggaag gtcagtctgc catgcaaagt
    12721 caggagcagg attcaaatgg agcggtaaaa tcaatcgtat caaaaagtcc aggagctatc
    12781 tcttatttat ctcttaccta tatagatgat tcggtcaaaa gcatgaagtt gaatggctat
    12841 gacttaagtc cagaaaatat aagtagcaat aattggccct tgtggtctta tgagcatatg
    12901 tatacattgg ggcagcccaa tgagttagct gcagaatttc tcaattttgt tctctcggat
    12961 gagacccaag aagggattgt caaaggattg aagtatattc cgattaagga aatgaaggtt
    13021 gaaaaagatg ctgccggaac tgtgacagtg ttggaaggga gacaataatg aatcaagaag
    13081 aattagctaa gaaaatgttg cttccatcaa agaattctcg tctggagaaa ttaggaaaag
    13141 gtttgacctt tgcctgtctt tctttgatag tcatccttgt ggccatgatt ttggttttcg
    13201 tagcgcaaaa aggcttgtcg accttctttg tcaatggtgt gaatatcttt gactttcttt
    13261 tgggaggaac ttggaatcct tctagtaaag aatttggtgc ccttcctatg attttgggtt
    13321 cctttatcgt taccattctc tcagccctta tcgcaacacc ctttgctatt ggtgcagcag
    13381 tttttatgac cgaagtatca ccaaaagggg cgaagatttt gcaaccagct attgaactcc
    13441 tggttgggat tccttcagta gtgtacggat ttattggctt gcaagtcgtc gttccctttg
    13501 ttcgcagtgt ctttggtggg actggttttg ggattttgtc agggatttcc gtcctctttg
    13561 tcatgatttt gccgaccgta acctttatga caacggatag cttgcgtgcg gttcctcgtt
    13621 attatcgtga ggcaagtttc gctatgggag ccactcgctg gcagactatc tggcgtgtga
    13681 tcttgaaggc ggcccgttct ggtattttca ctgcagtggt ctttgggatg gcgcgtgcct
    13741 ttggtgaggc tttagctatc cagatggttg tcggaaactc agctgttatc ccaacttcct
    13801 tgaccacacc agctgcaact ttaacttcta tattaactat gggaattggg aacactgtca
    13861 tgggaactgt aaataataat gttctctggt cactggcctt ggtactgctc ttgatgagtt
    13921 tagtttttaa cagtgtgatt aaattgatta cgaaagaaag aggaaagaaa aattatgcgc
    13981 gctaagaaat tagataaact tgcaacagct gtcctctata cgattgctag catcattgtg
    14041 acaatcttgg cttccttgat tctctatatc ttggttcggg gcttgcccca tatctcttgg
    14101 tctttcttga ctggaaggtc ttctgctttt caagcaggtg gtgggattgg cattcagctt
    14161 tacaattcct ttttcctatt ggtcattacc ttgattattt ctgtacctct ttctatggga
    14221 gctgggattt acttggctga atatgctaaa aaaggtcctg ttaccaactt tgtgcggact
    14281 tgtattgaaa tcttgtcctc tttaccatca gtggtggtgg gtctctttgg ttacttgatc
    14341 tttgtagtcc agtttgagta tggattttca atcatttcag gtgccttggc cttgacagtc
    14401 tttaacttgc ctcagatgac gcgtaatgta gaggatagtt tgaaacacgt tcaccatacc
    14461 caacgtgagg ctggtctggc tcttgggatt tctcgctggg agacagtggt tcatgttgtt
    14521 attccagaag cgcttccagg tattgtaacg ggtgtcgtct tggcatctgg tcgtatcttt
    14581 ggcgaagctg cagctctgat ctatacagca gggcaatcgg cgccagctct tgactggtct
    14641 aactggaata tcctcagtgt gactagcccc atctctatct tccgtcaagc agaaaccttg
    14701 gctgtccata tctggaaagt caatagtgaa ggcactattc cagatggaac cattgtatca
    14761 gcaggttctg ccgctgtgct cctgatcttt atcctgattt ttaactttgg agctcgtaag
    14821 ttcggaagct atctacacaa gaaattaacc gctgcctaaa ggagaagcca tgtcaacata
    14881 taactgggat gagaagcata tccttacctt tcctgaagaa aaagtagccc tttctactaa
    14941 ggatgtccat gtttactatg gtaaaaatga atccattaag gggattgata tgcaatttga
    15001 aagaaataaa attacagctt tgattggtcc gtcgggatcg gggaaatcta cctacttacg
    15061 cagtctcaat cgcatgaatg atactattga tattgctaaa gtaactgggc agattctcta
    15121 tcgtggaatt gatgtcaacc gtccagaaat caacgtttat gaaatgcgta aacacattgg
    15181 aatggttttt caacgcccca atccatttgc taagtcaatt taccgtaata ttacctttgc
    15241 gcatgaacgt gctggagtta aggataagca agtcctagat gaaatcgtag aaacctccct
    15301 tcgtcaggct gccctctggg atcaggttaa agacgatctc cacaagtcag ccttgacctt
    15361 atcaggtggt cagcaacaac gtctctgtat cgctcgtgcc atctctgtta agccagatat
    15421 cctcttaatg gatgagccag cctcagcctt ggatccgatt gcgaccatgc aactagaaga
    15481 gaccatgttt gagctcaaga aaaactttac catcatcatt gtaacgcata atatgcagca
    15541 ggctgctcgt gcaagtgact atacaggctt cttttacttg ggtgatttga ttgagtatga
    15601 caagactgca actattttcc aaaatgccaa gctacagtcc accaatgact atgtatctgg
    15661 tcactttggt tagaaaggaa accgtatgac agatgcgatt ttacaggtat cagacctgtc
    15721 cgtatattat aataaaaaga aggctttgaa tagtgtttcc ctatctttcc aacctaagga
    15781 aattacagcc ttgattggtc catctggatc agggaagtca accctcctca agtctctcaa
    15841 ccgcatggga gatctcaatc cagaggtgac cacaactgga tccgtggtgt acaatggtca
    15901 caacatctac agtccgcgta cagatacggt tgaattacgt aaggaaatcg gaatggtttt
    15961 ccaacaacct aatcctttcc ctatgactat ctatgagaat gttgtctacg ggcttcgtat
    16021 caatggaatt aaggataagc aggttctgga tgaagccgta gaaaaagcct tgcaaggtgc
    16081 ctctatctgg gatgaggtca aggatcgtct atatgattca gctattggat tgtcaggtgg
    16141 tcaacagcag cgtgtctgcg tggcccgtgt cttggcaact agtcctaaaa tcatcctctt
    16201 ggatgagcca acttcggctt tggatccgat ttcagctggt aaaattgagg aaaccttgta
    16261 tggtctaaaa gacaagtaca ccatgcttct ggtaacccgt tccatgcagc aagcttcacg
    16321 tatctctgat aagacaggat ttttcctaga tggagatttg attgaattta atgataccaa
    16381 gcagatgttc cttgatcccc aacacaagga aacggaagac tatattacag gaaaatttgg
    16441 ataaggagat gaaagatgtt acgatctcaa tttgaagaag atttagagaa attacataac
    16501 cagttctacg ctatgggaca agaagtgctc tcacaaatca atcgtacggt acgtgctttt
    16561 gtcacgcatg accgtgacct ggcaaaagag gtcatcgaag atgatgcaga agtaaatgaa
    16621 tacgaagtga aactggaaaa gaaatcattt gaaatgatcg cactccaaca accagtctct
    16681 caagatttgc gtacagtctt gactgtcctt aaggctgtat cagatgtgga gcgtatgggg
    16741 gatcacgctg tagccattgc ccaggcaacc atccgtatga agggggaaga acgcatccca
    16801 gctgtagagg aagaaattaa aaaaatggga cgtgaagtta aaagcgttgt ggaagcagca
    16861 cttgatcttt atcttaatgg ttctgttgac gacgcatacc gggtggcctc catggatgag
    16921 caaattaacc actattttga aactatccgt gaccttgcga ctgaagagat taagaaaaat
    16981 ccagaagcca ttgttacggg tcgtgattat ttccaagtta tttcctactt ggagcgtatc
    17041 ggtgactatg ctaaaaatat ctgtgaatgg gttgtctact ttgaaacagg taagattgtc
    17101 gaactataaa atagtttaat tctgtataaa aaggagcttg aaatcaactg atggctcctt
    17161 tttttgaacg aaaaaatatt tttgagataa aaatttaaaa aacacttgac aagtaacttg
    17221 gatatcgtta taattataca aaattaacag aaaggttgtt tatttatgaa atcaaaaaaa
    17281 atggatattt gttttatgta attttcttgc aagtttcttc ttagtagctt gccagtcagg
    17341 ttctaatggt tctcagtcag ctgttgaggc cattaagcaa aaggggaaat tagttgtggc
    17401 aaccagtcct gactatgcac cctttgaatt tcaatcattg gttgatggaa agaaccaggt
    17461 agtcggtgca gacatcgaca tggctcaggc tatcgctgat gaacttgggg ttaagttgga
    17521 aatcttaagc atgagttttg acaatgtttt gaccagtctt caaactggta aggctgacct
    17581 agcagttgca ggaattagtg ctactgacga gagaaaagaa gtctttgatt tttcaatccc
    17641 atactatgaa aacaagatta gtttcttggt tcgtaaggct gatgtggaaa aatacaagga
    17701 tttaactagc ctagaaagtg ctaatattgc agcccaaaaa gggactgttc cagaatcaat
    17761 ggtcaaggaa caattgccaa aagctcaatt aacttcccta actaatatgg gtgaagcagt
    17821 caatgaattg caggctggaa aaatagatgc tgttcatatg gatgagcctg ttgcacttag
    17881 ttatgctgct aaaaacgctg gcttagctgt cgcaactgtc agcttgaaga tgaaggacgg
    17941 cgacgccaat gccgttgctc ttagaaaaaa tagtgatgat ttgaaagaag tggtagacaa
    18001 ggtcatccaa aaacttaagg atgaaggaac ttaccaaagc tatcttgaaa aagcagcaag
    18061 tctaactgaa gttgaagaat aagaaaaagc agagttggat gtcattccaa ttctgctttt
    18121 aaatagttta aaacattttc cagattgtct agggacacct tggcaaattg ataaggacag
    18181 gtgctcaaat aggcctcttc aaagaagtcc aagcccagtt gactgtgttt gagactggcg
    18241 attttaggat actgtctcag gtctagcaat aaaccatcgt gtaggggata atgaggaagg
    18301 ggacaaggtg ttttagttat cctttcctcg atttgttttt ggtaagattc atgattggtc
    18361 aagcgagcca aacgattttt ttcaaacaat tgactgtcaa tatagagtga cagggccttt
    18421 tgcataatga ggttgctgtc gtagtctagg atatttttgt aggccacaaa tgcttctttg
    18481 atagcatttg gaagaatgag tgctgtaata cgaagggcag gaaaaaggct ggtcgagaag
    18541 gacttgatat aaatgacatg ctcctctgta tcaagatagt ggaaggtttg gcccttcttg
    18601 gagtccaaat cacccagata atcgtcctct acgatataga catcatactt ggcagctaag
    18661 ttaagaatag atcgtttgtc ttgctcagaa taggaatgtc ccagaggata gtgaaatcgg
    18721 ggaatggtgt agaaaaattt aatttttcct gttttgaagt ggccttccag ctcctccaag
    18781 tcaatcccat caatgcctcg ttcaatcgtt tgatagtcca gcccctgtgc aatcaagagg
    18841 cgattcatcc gatggtaggt tggctgttcc accaagattt ccttggcttg tctaggaaag
    18901 gatatttgag agaggataaa taaggcttgt tgggttccag aagtcagtac tagttggtta
    18961 gccttgcagt agagagcttg ctcaaagagg agtttgtgaa tggactgtct taggtcttct
    19021 aatccttctt gattgtcata gtagttgaag aggtagtttt ctcggccaat caaggtttca
    19081 ttgacacaga gtcggaaatc gtcataggca ctggcatgtt cgtcggtaac ctcgatttct
    19141 aggtcttgat gttgcccttg ttctaataca tagtagccac tctgaggctt ggcatagagg
    19201 tattgttcgt gccgtaattc cagcagggct cgttgaatgg tgtccttgct gcagtgaaag
    19261 tcaaggctca gttgacggat agaaggcagg cgactacccg tcggaaatcg tccagactcg
    19321 ataccatttt tcagatagga aacgacctct ttgtacttgc tttgtttctt cattccagac
    19381 ctcccttgat tttgttacat tgtacccttt tttttccttc ttggcaatgt ctagaacgtt
    19441 tctctcgttc acatctaggg gcaaatgtgg tatacttagg agtaagattt atagaataac
    19501 agacaagaaa gtgaatggat aagaacgtgc aggaaccagt gaaattattt caatacaata
    19561 ctcttggagc cttgatggct ggcctttatg gtggtactat gacagtagga gaattgctag
    19621 aacatggtga ccttggtttg ggaaccttgg attccattga tggggaattg attgtcttgg
    19681 atggcaaggc ttatcaggcc aaagggtcag gagaccagcc agaaattgtg gaagtgtcac
    19741 cagatgccct tattccttac gctgcagtgg taccgcatca ggcagaggtc attttccgcc
    19801 agcgctttga gatgacagac aaggaattga aagaacgaat tgagtcttat tatgatgggg
    19861 aaaatctttt ccgctctatc aagattcggg gggaattttt acatatgcat gtgcgtatga
    19921 ttcctaagtc aacacccgat accaagtttg ctgatgtcgc aacccatcaa ccggaatata
    19981 gtcgtgacaa tgttgcgggg actattgttg gtttctggac gcctgagatt ttccatgggg
    20041 tcagtgtggc aggctaccat ctgcacttca tatcagatga tttgaccttc ggtggacatg
    20101 tcatggattt tgtcatcaag gaaggcatta tcgaggtggg agcagttgac cagttggacc
    20161 aacgtttccc agtccaagac cgtcaatact tgtttgctaa gttcaatgtt gacgagatga
    20221 aaaaagatat tgaaaaggca gaataggaga agaaaatgac cattcatatc attattacca
    20281 tgttgctgtt gctagctttt ctgataggaa gcatttggtt tgccaaaaag aaataccaga
    20341 ttaatctagc tgtcttgggc ttgggtgcag tcgctttctt tgtctcttcg cagattttgg
    20401 aaaaactggt gcatatcttg attttgcacc ctcaaaaaga cggtagtatt gccctcttgc
    20461 aagatcatcc gcttatctat attatctatg gcctagccat ggcagcattt tttgaggaaa
    20521 cggctcgtct tgttttcttc aaatggtttg agaaaaagag aagtttggaa aaggcagacg
    20581 ccttggccta tggactgggg catggtggct tggagttgat ttttctaggc cttactagtt
    20641 tactcaatct ttacattgtt ctttcggcag ttcaaactca gaatccacag gttatgcaat
    20701 tactgtctga aaatatgtta aaaaccattc agtcactttc ggtctggcag atttatttgc
    20761 ttggttttga gcgaatcttg gcgcttggtt tccaattact tttgacagtt tgggtttacc
    20821 aagctgttcg ccagaagaaa tggatttatc tcctaacagc ctatggcttg catgccttct
    20881 ttgatctggc accatctctt ttccaagtag gctggttgac aaatccagtc ttggttgaag
    20941 tgattctagc actggagctc gttctggtcg cctatggaac caaggaaatc ttttgtaaaa
    21001 aatcataaaa aggggggaac ctctttttct tatgcaaaat ccaaacaagg tatttttatg
    21061 gtcgtcaaat gtctctaaaa atggtataat ggaatgaatt ttggaaaagg aagagtgaca
    21121 tgtctgtaag agaaaaaatg cttgaaatct tagaaggaat tgatatccgt tttcaggaac
    21181 ccttgcatag ctatagttat acaaaagtag gtggagaggc tgattatttg gtctttccac
    21241 gaaatcgttt tgagttggct cgccttgtga aatttgccaa ccaagaaaat atcccttgga
    21301 tggttcttgg caatgcaagc aatatcatcg ttcgtgatgg tgggattcgt ggatttgtca
    21361 tcttgtgtga caagctcaat aacgtttctg ttgatggcta taccattgaa gcagaagctg
    21421 gggctaactt gattgaaaca actcgcattg ccctccgtca tagtttaact ggctttgagt
    21481 ttgcttgtgg tattccagga agcgttggcg gtgctgtctt tatgaatgcg ggtgcctatg
    21541 gtggcgagat tgctcacatc ttgcagtctt gtaaggtctt gaccaaggat ggagaaatcg
    21601 aaaccctgtc tgctaaagac ttggcttttg gttaccgcca ttcagctatt caggagtctg
    21661 gtgcagttgt cttgtcagtt aaatttgccc tagctccagg aacccatcag gttatcaagc
    21721 aggaaatgga ccgcttgacg cacctacgtg aactcaagca acctttggaa tacccatctt
    21781 gtggctcggt ctttaagcgt ccagtcgggc attttgcagg tcagttaatt tcagaagctg
    21841 gcttgaaagg ctatcgtatc ggtggcgtag aagtgtcaga aaagcatgca ggatttatga
    21901 tcaatgtcgc agatggaacg gccaaagact acgaggactt gatccaatcg gttatcgaaa
    21961 aagtcaagga acactcaggt attacgcttg aaagagaagt ccggatcttg ggtgaaagcc
    22021 tatcggtagc gaagatgtat gcaggtggtt ttactccctg caagaggtag tggggacctg
    22081 acagagcccc gatcggttaa tctatgaaaa agaaggaatt tatgccaatt gaaaaaacca
    22141 attatcgaat tcaaaaacgt ctctaaagtt tttgaagaca gcaacaccaa ggttctcaaa
    22201 gacatcaact ttgagttgga agaagggaaa ttctacaccc ttctaggtgc atctggttcg
    22261 gggaaatcaa ctatcctaaa cattattgca ggtttactgg atgcgacgac aggagatatc
    22321 atgctagacg gtgttcgtat caatgatatt ccaaccaaca agcgcgacgt acataccgtc
    22381 ttccaatcct atgccttgtt cccacatatg aatgtgtttg aaaatgttgc ctttccactt
    22441 cgcttgcgta aaattgataa gaaagaaatc gagcagcgtg tagcggaagt tctcaagatg
    22501 gttcagttgg aaggttatga aaaacgttcc atccgcaaac tttctggagg acaacgtcag
    22561 cgtgtggcca tcgcccgtgc tatcatcaac caaccccgtg tggtcttgtt ggacgagcct
    22621 ttatcagcgc tggacttgaa attgagaaca gacatgcagt acgaattgcg tgaattacaa
    22681 caacgattgg gcattacctt tgtctttgtc actcacgatc aggaagaagc tcttgccatg
    22741 agtgactgga ttttcgttat gaatgatggc gagattgtcc agtctggaac ccctgtggac
    22801 atctacgatg agccaatcaa ccactttgtt gccaccttta tcggggagtc aaacatcttg
    22861 ccaggtacca tgattgagga ctacttggtc gaatttaacg gcaaacgctt tgaagcggtt
    22921 gatggtggga tgaagccaaa tgaacctgtt gaggtcgtta ttcgtccaga ggacttgcgc
    22981 attacccttc ctgaagaagg caaactccaa gttaaggtcg acacccagct cttccgtgga
    23041 gttcattatg aaattatcgc ctatgacgaa cttggaaatg aatggatgat ccactcaacc
    23101 cgtaaggcca tcgtgggtga ggaaatcggt ctggactttg aatcagaaga catccacatc
    23161 atgcgtctca atgaaaccga agaagagttc gatgctcgta tcgaagaata cgtggaaatc
    23221 gaagagcaag aagcaggtct gatcaatgcg atcgaggagg aaagagatga agaaaacaag
    23281 ctctaaactc tttgtagtgc cctacatgct ttggattgcc ctctttgtat tggcaccctt
    23341 ggtcttgatt ttcggacaat cctttttcaa cattgaaggc cagtttagtt tagaaaatta
    23401 taaatcttac tttgcgtcac aaaacttgac ctacctcaaa atgagtttca actcagtgct
    23461 ttatgcaggg attgtgacct ttgtgacctt gcttatcagc tatccaacag ccctcttttt
    23521 gacccgtctc aagcaccgtc aactctggct catgctgatt atccttccta cctggatcaa
    23581 tttgctcctt aaagcctatg cttttatcgg gatttttggt caaaatggct ctattaacca
    23641 attcttggaa tttatcggaa ttggttcaca acagttgctt tttaccgatt tttcctttat
    23701 ctttgtcgca agctacatcg agctcccctt tatgattttg ccgattttca atgtcttaga
    23761 cgacatggat aataatctca tcaatgctag ttatgacctt ggtgcaacta agtgggagac
    23821 cttccgtcat gtcatcttcc ctctatctat gaacggtgtg cgaagtgggg ttcagtcggt
    23881 ctttatccca agtttgagtc tcttcatgct gacccgtttg attggtggga accgcgttat
    23941 caccttgggg acggctattg agcagaattt tctaaccaat gacaactatg gtatgggttc
    24001 aaccatcggt gtgattctca tcctgaccat gttcatcacc atgtgggtga ctaaggaaag
    24061 gagagaacga tgaaaaaatt tgccaacctt tatctgggac tggtctttct ggtcctctac
    24121 ctgcctatct tttacttgat tggctatgcc tttaatgctg gtgatgatat gaatagcttt
    24181 acaggtttta gctggactca ctttgaaacc atgtttggag atgggagact catgctgatt
    24241 ttggctcaga catttttctt ggccttccta tcagccttga tagcgaccat tatcgggact
    24301 tttggtgcca tttacatcta ccagtctcgt aagaaatacc aagaagcctt tctatcactc
    24361 aataatatcc tcatggttgc gcctgacgtt atgattggtg ctagcttctt gattctcttt
    24421 acccaactca agttttcact tggctttttg accgttctat ctagtcacgt ggccttctcc
    24481 attcctatcg tggtcttgat ggtcttgcct cgactcaagg aaatgaatgg cgacatgatt
    24541 catgcggcct atgacttggg agctagtcaa tttcagatgt tcaaggaaat catgcttcct
    24601 tacctggctc cgtctatcat tactggttat ttcatggcct tcacctattc gttagatgac
    24661 tttgccgtga ccttctttgt aacaggaaat ggcttttcaa ccctatcagt cgagatttac
    24721 tctcgtgctc gcaaggggat ttccttagaa atcaatgccc tgtctgctct agtctttctc
    24781 tttagtatta tcctagttgt aggttattac tttatctctc gtgagaagga ggagcaagca
    24841 tgaaaaaaat ctattcattt ttagcaggaa ttgcagcgat tatccttgtc ttgtggggaa
    24901 ttgcgactca tttagatagt aaaatcaata gtcgagatag tcaaaaattg gttatctata
    24961 actggggaga ctatatcgat cctgaactct tgactcagtt tacagaagaa acaggaattc
    25021 aagttcagta cgagactttt gactccaacg aagccatgta cactaagata aagcagggtg
    25081 gaacgaccta cgatattgcc attccaagtg aatacatgat taacaagatg aaggacgaag
    25141 acctcttggt tccgcttgat tattcaaaaa ttgaaggaat cgaaaatatc ggaccagagt
    25201 ttctcaacca gtcctttgac ccaggtaata aattctccat cccttacttc tggggaacct
    25261 taggaattgt ctacaacgaa accatggtag atgaagcgcc tgagcattgg gatgaccttt
    25321 ggaagccgga gtataagaat tctatcatgc tctttgatgg ggcgcgtgag gtgctgggac
    25381 taggactcaa ttccctcggc tacagcctca actccaagga tctgcagcag ttggaagaga
    25441 cagtggataa gctctacaaa ctgactccaa atatcaaggc tatcgttgcg gacgagatga
    25501 agggctatat gattcagaat aacgttgcaa tcggcgtgac cttctctggt gaagccagcc
    25561 aaatgttaga aaaaaatgaa aatctacgtt atgtggtacc gacagaggcc agcaatcttt
    25621 ggtttgacaa tatggtcatt cccaaaacag ttaaaaacca agactcagcc tatgccttta
    25681 tcaactttat gttgaaacct gaaaatgctc tccaaaatgc ggagtatgtc ggctattcaa
    25741 caccaaacat accagcgaag gaattgctcc cagaggaaac aaaggaagat aaggccttct
    25801 atcccgatgt tgaaaccatg aaacacctag aaatttatga gaaatttgac cataaatgga
    25861 cagggaaata tagcgacctc ttcctacagt ttaaaatgta tcggaagtag gagataaacg
    25921 taagaagacg aatcagtcaa gctggttcgt ttttaaggaa tagaagtcag tttttagagc
    25981 cctatctttt tctattctag ttgtttattt gcttatttgc ttgcctagta gtatactaag
    26041 cctatactct tcaaaccacg tcagcgttgc cttgccgtac tcaagtacag cttgaggcta
    26101 gcttcctagt ttgctttttg atttttattg agtattaacc ttacagaaag cggcaacaaa
    26161 taatgaaaat gtctactttt ttcaacaaaa gtttctggcc aacctttatg attgttaacc
    26221 aaactgctat actattccat ttaaaggatg gcttggatcg acaataccta acaactgagt
    26281 ccatatattg ggtgataggc acctttatat ttggaaatat tctagtagct gtgtttagta
    26341 atatgaaaat ttgggataaa aagaaaaacg gcagtaagaa aaaatatatc ctggaaaagt
    26401 aatcagagat gtagggaatt ggtctaattc aacggagatc atttccagtt gacgcaagat
    26461 taaaaaaacg atataataag aaagttgaga attgattttc acttgtctta gtccagagaa
    26521 atggcggtgc tgcgagccat ctaagctagg aagtcatgct actcaatcat acaattgcat
    26581 aagaacaaga gaatgacaag ttcattgaat gaaggtggta ccgcggtttt tcgcccttcg
    26641 tgatatgagc ttgtcttttg atttttggag gtgttgatga aaacatttct tgtcaaacaa
    26701 aagtttcgtc ttggaggcga acgcttcgct atcaaggatg acaggggaga aattgcctat
    26761 caggtggagg gatcattttt taagattccc aaaactttta ccatctatga tgcggctggt
    26821 gaacaagtca gtcagatcag taaagaaatc ttgaccttgc ttcctcgttt tgagattcag
    26881 cttctggatg gctcgagttt tgtcattcgt aagaagttga ccttttggcg agataagtat
    26941 gagtttgata atctaggtct tcgtatcgag ggcaatatct gggatttgaa tttcaaattg
    27001 ctggatgatc gcgatcagtt gattgcggaa attaagaagg aactcttcca tctgacctct
    27061 acctataccg taacggttct tgaagacgct tatgcagacc tagtcatttc cctctgtgtc
    27121 gcgattgact atgtggaaat gctggaaagc caatcacatt aaacaagtaa ataaggagac
    27181 aatatgaaac aactatctag tgcacaagta cgccaaatgt ggcttgattt ctgggcgacc
    27241 aaaggtcact cagtagaacc atcagtaagt ttggttcctg taaatgaccc aactcttttg
    27301 tggatcaact ctggggtagc aacccttaag aaatactttg acgggaccat tatccctgaa
    27361 aatccacgta ttaccaatgc ccaaaaggct atccgtacca acgacatcga aaacgtaggg
    27421 aagactgcgc gtcaccatac catgtttgaa atgttgggga acttctctat cggggattac
    27481 ttccgtgacg aagctatcac ttgggcttat gagcttttga taagccctga atggtttgat
    27541 ttccctgctg aaaaacttta catgacctac tatccagacg ataaagattc ttacaaccgc
    27601 tggattgaag tgggagtgga tccaagtcac ttgattccaa ttgaggacaa cttctgggaa
    27661 atcggtgcgg gaccttctgg accagataca gaaatcttct ttgaccgtgg ggaagccttt
    27721 gacccagaaa atatcggtct tcgcctgctt gcagaagata ttgaaaacga ccgttatatt
    27781 gaaatctgga acatcgtttt gtcacaattt aacgcagacc ctgctgttcc tcgtagcgaa
    27841 tacaaggaat tgccacataa gaacattgat acgggcgctg gtttggagcg tttggtggcc
    27901 gttatccaag gggctaagac caactttgaa acggacctct tcatgccgat tatccgtgaa
    27961 gtcgagaaat tgtctggtaa ggtttatgac caagatggcg acaacatgag cttcaaggtc
    28021 atcgctgacc acatccgttc actttcattt gccatcggtg atggtgccct tccaggaaat
    28081 gaaggtcgtg gttatgttct tcgtcgtctt ctccgtcgtg cttctatgca tggtcaaaaa
    28141 ttgggaatca acgagccttt cctttacaaa ttggttccaa ctgttggaaa aatcatggaa
    28201 agctactacc cagaagtgct tgagaaacgt gactttattg agaaaatcgt taagagcgaa
    28261 gaagaatcat ttgcccgtac ccttcactca ggtcaacact ttgcccaagg cattgtagca
    28321 gacttgaaag aaaaaggtca atctgttatc gctggttcag atgtcttcaa actctatgat
    28381 acttatgggt tcccagttga attgactgaa gaaattgctg aagaggctgg tatgactgta
    28441 gaccgtgaag gttttgaagc agccatgaaa gaacaacaag aacgcgcgcg tgcgtcagct
    28501 gtcaagggtg gctcaatggg tatgcaaaat gaaactcttc aaaacatcac tgtagaaagt
    28561 gtcttcaact acaatactag ccaattgtct tctaaattgg tagctatcgt tgctgacaat
    28621 gcagaagtag gagctgtttc agaaggaact gcctctctta tctttgcgga aacgtcattt
    28681 tatgctgaaa tgggtggaca ggtagctgac tacggacaaa tcttggatga gtcaggtaag
    28741 attgtggcta ctgtgaccaa tgttcagaaa gccccaaatg gtcaagccct tcatacagtt
    28801 gaagtccttg caccgcttgc cttgaaccaa gaatatacct tggcaattga tagcaatcgc
    28861 cgtcaccgtg tcatgaaaaa ccacactgcg actcatttgc ttcacgctgc ccttcacaat
    28921 atccttggaa accacgcaac acaggcagga tctcttaacg aagttgaatt ccttcgcttt
    28981 gactttaccc acttccaagc agtaactgct gaggaacttc gtgcgattga acagcaagtt
    29041 aacgagaaaa tttgggaagc acttgaagtg aagacagttg aaacggatat tgacactgct
    29101 aaagaaatgg gagctatggc cctctttggt gagaaatacg gcaaggaagt tcgtgtcgtg
    29161 actatcggtg actactctat tgaactttgt ggtggtactc atgttggcaa cacttctgag
    29221 attggtctct tcaaaattgt caaagaagaa ggaatcggtt caggaactcg ccgtatcttg
    29281 gcagtgactg gtaaggaagc ctttgaagcc tatcgtgaac aagaggatgc tcttaaagct
    29341 gtcgcagcaa ccttgaaagc acctcaagtc aaggaagtac ctcacaaggt agaaggactt
    29401 caagaacaac ttcgtcaact tcaaaaagaa aatgctgagt tgaaagaaaa agccgcagct
    29461 gcagccgcag gcgatatctt caaagatgct aaggaagtca acggtcatcg ttacattgct
    29521 agtcaagtgt ctgtatccga tgccggtgcc cttcgtactt ttgcagataa ctggaaacaa
    29581 aaagactact ctgatcttct tgtcctagtt gccgctatcg gtgacaaagt caatgtcctt
    29641 gtagcaagca agacaaaaga ccttcatgca ggaaaccttg tcaaagaatt agcaccaatc
    29701 atcgatggac gtggtggtgg taaaccagac atggccatgg caggaggaag caaccaagct
    29761 aagatccaag aattgttgga tgcagtagca ggtaaattgt aatcaagtaa agatctatcc
    29821 atttgggtag gtctttttgt gaatacaaaa aagccaaatc cgcttggacc tagcttggtt
    29881 attatgagct attagatggt attgactgcc cagacactta cggatctagc tgagacaggg
    29941 aattgtccat aaccttcctc atcaattgta acttgacctt ggtggttacc aagtaaatct
    30001 acaaaggttt gattagtcca ttcttgaccg acaaacattg acttgctgtt ttcttggtca
    30061 tttgagataa ggactgcgat tggggattga ttttcagcac ctgaacgtac ccaaccgata
    30121 cagttagcat ggtcaaagta gtcattttgt tctccatagg ccaagtcttt tcggatggct
    30181 aggaggcggt caaggatttc tttgaaatct tcttgagcat actggcctga aatcccatag
    30241 tagtctccgt aaaagacaca tggaaggccg tcttggcgta acaaaatgag ggcataggct
    30301 gctggcttga accattcttc aacggtagac tcaagggctt gtcctcgttg ggtatcgtgg
    30361 ttgtcgacaa aagtcacagc cttgtcaggc ttgagttcaa ccaggctatc tgtgaaaatg
    30421 ccacgaaggt catagtttgc gccagcttga ctggcttcaa agagattctg gtggagacga
    30481 acatcgacaa ggtcaaagtg ttcttccgtt ttttcgagat agtccagatt ggcttccttg
    30541 tctgggttcc aaaattcacc aaaaacatag aaatcgtcac cgtatttttc cttcatatcg
    30601 cggatgaagt tgcgcataaa gaaagagtca atatgcttaa cggcatccaa acggaaacca
    30661 gctacaccag tcgtttccat gaaccaatca gcccagtcat agatgttttg gatgacttca
    30721 ggatgtttaa agtctaggtc ggcatacatg aggtagtcgt agtttccgtt ttcgttatcg
    30781 accaattcct cgttagccca gcccttgttg tccccttgga tcagataaat cccagattta
    30841 ctgcgtttgg catcgtagtc tgtaccggtg aagtggtacc aatgccagtg gaagccatta
    30901 taggtatctt ggcgaccatc gaaggtaaaa ctagtccagc cattgatggt gaagggttct
    30961 ccaagttcaa ctgtacggtc tacaggatca acttcgataa cctgaaaggc ttccctgtta
    31021 tcggcagcag ccttgtggtt gagaactaca tcggccatag gttgaattcc ctgttcttta
    31081 agggcttgaa tggcttgaag atagtcttct ttgaaaccat acttggtgcg gacagtccct
    31141 ttttggttga actctcctaa gtcaaataag tcatagaccc catagccgac atctttttcg
    31201 ttggttgcct tgaaggctgg tggcatccag acgtgactga tccccagatg agctaggtgt
    31261 ggagcatttt cagccagacg cgtccagtgt tgaccgtcgt ggggcagata ccattcaaag
    31321 tattgcatga gtgtttgatt ttgcatgatg tttcctctta cttgtcaatc ttgttcttta
    31381 ttctatcata aagtatctct cggcgcaaac gtttgcgtaa gatagaagga aactatttta
    31441 actacttaaa ataaagtatt gatttttttg tatcaaagtg ctagtatagc gatgtataat
    31501 acagataaag ataggagttg tcccatgatt gaatttcaaa atgttagtaa gttgtatggt
    31561 gataaagagg ccttgagtaa tctcaatttg cagattgaaa atggagagat tatgggcttg
    31621 attggtcata atggggctgg aaaatcgacc accataaaat ccctagtcag tatcatttca
    31681 cccagcagtg gtcgtatttt ggtagacggt caggagttat cggaaaatcg cttggcgatc
    31741 aaacgaaaga ttggctacgt agcagactcg cctgacttat ttttacgctt aacggccaat
    31801 gaattttggg aattgatcgc ttcatcctat gatctgacta gctctgactt ggagactagt
    31861 ctagctaggc tattgaacgt ttttgatttt gctgaaaacc gctatcaggt tattgaaact
    31921 ctttctcacg gaatgcgtca gaaagtcttt gtcattgggg cactcttgtc tgctcctgat
    31981 atttgggttt tggacgaacc cttgactggt ttggatcccc aggccgcctt tgatttgaag
    32041 caaatgatga aggaacatgc acaaaaaggc aagacggtct tgttttcaac tcatgtcctt
    32101 gaggtagccg agcaagtctg tgatcggatt gctattttga aaaaggggca tttgatctat
    32161 tgtggtagtg tagagaactt gaggaaagac cacccagacc agtttttgga aagtatctac
    32221 cttagtcttg ctggtagaaa agaggaggtt gcggatgcgt ctcaaggtca ttaaaaaatt
    32281 agttgatatc aatatcctct attcatctca agaagctaat ctggctaatc tacgaaagaa
    32341 gcaggctaag aatcctggga aaaaagtaaa tgtttctgct agagtcctaa gttcttacat
    32401 tttttccagt ctcttgatgc tcatctgttt tagtaatata gccattcatt ttccttttga
    32461 ggaaatacct atttatttta gctcgatggt tgctatttta ctggtgctgg ccttttcaac
    32521 ttctttcact gccttttaca atgtctttta tgagagtaag gacctggcct cctataggcc
    32581 ctatgccttt aaagaatcag agattataat ggccaaaggt ctgtctgtcc tcttgccagc
    32641 tctaactgga attgtaccaa tcctagctta ttttctagcc ctctatatta gtctagctcc
    32701 ttctctttgg ctgggtttgc ctttgatgct actgtccttg accttattat ttgtctctgt
    32761 tactttagtg acggtagtgg cagtacattt cttggctcag actagggtct tcagaaagta
    32821 tcagtctatt ttttcgaatg tgatgattgg gataggagtt ctcatacctt taatatttat
    32881 cttctttctt cagtcgactt ttggaagtat tgttgacaaa gttagagaca ttccatttct
    32941 cctttatcct cttcatatct tttacaaaat agcagtggag cctttttcga cagaagcctt
    33001 agtgggtctg ctcgcttgga taggactaac tctcttcctg ctttatctga ccaaaaagaa
    33061 ggtccttcct cgtttttatg acgtgatcct gcttaacagt gaggagaagg tcaaaaaaga
    33121 acgtcgcagc aaggagagga tttcaactac taaaaagggc tttttccgta tggttttacg
    33181 ctaccacctc accctcttgg gataggggac tggtgtgatt acagtgcttt ttacaagtgc
    33241 tttccttcct tatctcatga tgatcagtct gatttccaaa atccgagatt ctcagatagt
    33301 tccagacatt catcctccat actggttacc cttgtttttt gtaggagtgt ttatagcagt
    33361 tgtcaataac aatatcacca gcctgcattc aattgccttg tccttggaga gggaaaatgt
    33421 tgattttctg aagagtttac cctttgactt tgctcgttat gtgaaagtga aattttggat
    33481 tatctttgct gttcagtcct ttttaccagt tctgactttg ctcggtcttt ctctatatct
    33541 aggcttgtcc atcctttcga tgatttacct tcttgtagtc tggatcattg ccagtgtcat
    33601 cctttcttgc caccattact ttaaggacgt taaaaatctg tcaaccaatt ggagtagcat
    33661 tacggacctg gtgaatcgtt caaatggcat agtcgccata gttttattgt ttatttatag
    33721 tgcaatcctg atggcccttg taattgggag catattcttg gttcagtctc tctcccctat
    33781 ccttgccatc aacttgggag taggagctct tatcgtcttg cttgctcttg ctatttttgg
    33841 ctatcattat tacctgtcac gcatattggc agaaatagaa aaaagatgag atagttggtt
    33901 gcttgcttga taaattcaat tcgacaaacg cttttatagt ttgttagaat gtagttgtaa
    33961 atgattaaga ttggtaatca aaaatatagg gaggaatgtt ttgaataaga aatataatat
    34021 agttctatta ttgttgttta tagtttattt attgggatat ttttcaattt caaaaacgtt
    34081 aattcctata atgtgtgtga tccaagtatt tttgatagaa catatattta gagttcgaaa
    34141 tagaatgatg caaataggtg aaattttaat tattgttgct tctataatat tatttattga
    34201 tagtatattg agtttgtaac aaaaggcttt tctataaaat caaattttcc agatatctac
    34261 tgaagcagat ttatttcttc atctttcttc tactaccttt gtcactaaat ttgacagaca
    34321 catattgctg attcaacctc tctacctata atctcacagt taacctgagg ctagtttcat
    34381 agtttgttat ttgattttcg ttgataaaaa gaaatgctcc tccaagacgg aagagcattt
    34441 tttattgtga ttcttacaca tttacttcct agttttgcag gaagtctagt ataccatttt
    34501 ttaaaagaag agtaaaaaag caagggtagt ttcttttttg agaattgtct aaaagtttga
    34561 tataatggta gttatgaata gaataagagt cagcaaaagg gttgaaaaga agcttgctaa
    34621 ggggctagtt ttactagaag ccagtgatct tgagaatgtc aatcttaagg atcaggaagt
    34681 agaggtgcag ggtcaggaag gaaactttct tgggactgcc tacctttctc agcaaaacaa
    34741 gggcttgggc tggtttgtca gcaaagacaa ggtggccttc aatcaagctt tctttgaaac
    34801 gttgtttaga aaagccaaag aaaagagaaa cgcctactat caagatgatt tgacaactgc
    34861 ctttcgtctc tttaatcaag agggagatgg ctttgggggt ctgacagtgg acctttatgg
    34921 cgactacgcc gtcttttctt ggtataactc ttatgtttat cagattcgtc agactatatc
    34981 agaagccttt agacaggttt tccctgaggt tttaggggct tatgagaaaa tccgctttaa
    35041 gggtttggac tatgaatctg cccatgttta tggtcaagaa gcacctgact ttttcactgt
    35101 tttagaaaat ggtgtcctgt atcaagtctt tatgaatgat ggcttgatga caggaatttt
    35161 cctagaccag catgaggttc gcggtagttt agttgacggc ttggctatgg gtaaatcctt
    35221 gctcaatatg ttttcctaca cagcggcttt ttcagtagct gcggccatgg gaggagctag
    35281 ccagacaact tctgttgatc tagccaaacg ttcacgagaa ttgtctcaag cgcattttca
    35341 ggcaaatggg ctcagcacag acgagcgtcg ttttatagtc atggatgtct ttgagtattt
    35401 caaatatgcc aaacgcaaaa acttgaccta cgatgtgatt gtcctagatc cgcctagctt
    35461 tgctcggaat aaaaaacaaa ctttctctgt ggccaaggat tatcacaagt tgatttccca
    35521 gagtcttgag attttaaatc cgggagggat tatcattgcc agtaccaatg ctgccaatgt
    35581 ttcccgtcag aaatttacag aacaaattga taaaggcttt gcaggaagaa gttaccagat
    35641 tttaaacaaa tatggtcttc cagcagattt tgcctataat aaaaaagatg aaagtagtaa
    35701 ttacctcaag gtgattagta tgaaggttag taaatgaaat taatcgtttc agtaatgcca
    35761 agaagtttag aggaggctca ggctctggat gccacgaggt acctggatgc cgacatcatt
    35821 gaatggcgtg ccgactatct gcctaaagaa gcgattttgc aggtagctcc agccattttt
    35881 gaaaaattcg caggccgtga gttggttttc acgctacgaa ctcgctccga aggtggagaa
    35941 atcgaccttt ctccagaaga gtatatccat ctaatcaagg aagttgcgca actctatcaa
    36001 ccagactata ttgattttga gtactatagc tacaaggatg tttttgagga aatgctggac
    36061 ttcccaaatc ttgttttgag ttaccataat ttccaagaaa cacctgagaa catgatggaa
    36121 atcttgtcag agttgacgat cctaaatcca aaacttgtta aggttgcggt gatggctcac
    36181 acggagcagg atgtcttaga cttgatgaac tatacacgag gctttaaaac gctcaatcct
    36241 gaacaggaat atgtgaccat ttctatgggt aaggtgggca aggtctctcg tatcactgcg
    36301 gatgtgactg gttcgagttg gtcttttgcc agtctggatg aggttagtgc ccctgggcag
    36361 atttctctag ctagcatgaa aaaaatcagg gagattttgg atgaagcttg atggctatac
    36421 acgtttagct gcagttgttg ccaatcctat taagcattct atttctccct tcatccacaa
    36481 tagagccttt gaggcgacag ctaccaacgg tgcttatgtg gcttgggaga ttgaagcgag
    36541 tgacttggta gaaacagtgg ccaatattcg tcgctaccag atgtttggca tcaatctgtc
    36601 catgccctat aaggagcagg tgattcctta tttggatgag ctgagcgatg aagcgcgctt
    36661 gattggtgcg gttaatacgg ttgtcaatga gaatggcaat ttaattggat ataatacaga
    36721 tggcaaggga ttttttaagt gcttgccttc ttttacaatt tcaggtaaaa agatgaccct
    36781 gctgggtgca ggtggtgcgg ctaaatcaat cttggcacag gctattttgg atggcgtcag
    36841 tcagatttcg gtctttgttc gttccgtttc tatggaaaaa acaagacctt acctagacaa
    36901 gttacaggag cagacaggct ttaaagtgga tttgtgtgct ttagaatatg tttctgaact
    36961 gcaagcaagg attgccgagt cggatttgct agttaatgcc accagtgtgg gcatggatgg
    37021 ccaatcctct cctgttcctg aaaacatagt cttaccagaa actctcttag ttgcagatat
    37081 catataccaa ccctttgaaa caccgttttt gaaatgggct agaagtcagg gcaatccagc
    37141 agtcaatggt ctgggaatgt tgctctatca agctgcagaa gcttttcaac tgtggacagg
    37201 caagaaaatg ccgacagaag agatttggca gtctttaaca gaaaaatatc aataaagaaa
    37261 gggagactct cctatgaaaa tcagaatcga tattcctcat catccttatg atattcagat
    37321 tgaaaaaggt tgtatggccc aggctggtca gtggttgcga gaactctggc aaccgcaaaa
    37381 ggtagtcatt gtgacagata accatgtagc ctctctctat gcagagaagg tcaagctcag
    37441 cctagaagat gctggttttc aggtagctgt ttttgatttc ttagaaggtg aagaaagaaa
    37501 gaatttaact actgttcaga aagtctatga atttctagtc aagcaaggtc tgactcgtag
    37561 cgatggaatc gttgctcttg gtggtggcgt tgttggggac ctggctggct ttgtagcctc
    37621 tacctatatg cggggtattc actttgttca gattccgact agtttgacag ctcaggttga
    37681 ttcttctatc ggtggaaaga caggcgttaa cacaccattt gctaaaaata tggtggggac
    37741 ctttgcccaa ccagatgggg ttctgattga tccacttgtt cttgaaaccc tcggaaaaag
    37801 agagttgatt gaagggatgg gtgaagttat caagtatggc ttgattgagg atccagaact
    37861 gtgggctctc ttgacgggac tgaatggttc tgttgagaat attttggaac atgcagagac
    37921 cttgattgaa cattcttgtc aggtgaagcg caagatggtg gttgaagatg agttggacaa
    37981 tggtattcgt ctttacctca attttggcca cactattggc catgccatcg aagcgactgc
    38041 cggttatggc aaggtcatgc atggagaggc tgttgccatg ggaatggtac agatttccaa
    38101 ggttgctgag gaaaaaggcc tcatgccagc tggcattacc caatctatca cagagatgtg
    38161 tcagaaattc ggcttgcctg ttgactatga aaattggaaa gttgacaagc tttatcaggc
    38221 tcttactcat gacaagaaag cgcgtggtaa caccttgaaa ttggtcttgg tgccagagct
    38281 tggttcagcg accattcacc cagtttctct ggaagagatg aaagactact tggtaaaata
    38341 aggagagtgt atgagatatt taactgcagg agaatcacac ggcccccgtc taacagctat
    38401 tattgaggga attccagctg gacttccatt gacagctgag gatatcaatg aggatcttag
    38461 acgccgtcag ggtggctacg gtcgtggtgg tcgtatgaag attgagagtg accaggttgt
    38521 ctttacttcg ggtgttcgcc acgggaagac gacaggggcg cctattacta tggatgtcat
    38581 caataaggac caccagaaat ggctggacat catgtctgcg gaggacattg aagaccgcct
    38641 taaaagcaag cggaaaatta ctcatcctcg cccaggtcat gccgatttgg ttggggggat
    38701 taagtaccgt tttgatgatt tgcgaaattc tttggagcgt tcatcagctc gtgaaaccac
    38761 catgcgggtg gcagttggtg cagtagccaa acgcctcttg gctgagctgg atatggagat
    38821 tgccaaccat gtcgtggtct ttggtggtaa ggaaatagat gttcctgaaa atctgacagt
    38881 cgctgaaatt aagcaaagag ctgcccagtc tgaagtttct attgtcaacc aagaacgaga
    38941 acaggaaatc aaggactata ttgaccaaat caaacgtgat ggtgatacca tcggtggggt
    39001 tgtggagaca gtcgtcggag gcgttccagt tggtcttggt tcctatgtcc aatgggatag
    39061 aaaattggat gcaagattgg ctcaagctgt tgtctctatc aatgccttta aaggggtgga
    39121 atttggtctt ggctttgagg ctggttatcg taaaggcagc caagttatgg atgaaattct
    39181 ctggtctaaa gaagacggtt atactcgccg taccaataat ctaggtggtt ttgaaggtgg
    39241 tatgactaat gggcaaccca tcgttgttcg tggggtcatg aaacccattc ctactcttta
    39301 taaacctctt atgagtgtgg atatcgaaac ccacgaacct tacaaggcaa ccgtggagag
    39361 aagtgatccg actgctcttc cagctgcagg aatggtcatg gaagcagttg tagcaacggt
    39421 tctggcgcaa gaaatcctcg aaaaattctc atcagataat cttgaggaac taaaagaagc
    39481 ggtagccaaa caccgagact atacaaagaa ctattaagga gttcctatgg caaaaacaat
    39541 ctatatcgca ggtcttgggt tgattggagc ctctatggca cttggtatca aacgcgatca
    39601 tccagattat gaaattttag gttataatcg tagtcaagct tcgagagata tcgccttgaa
    39661 agaaggtatg attgaccgtg caacggatga ttttgctagt tttgctcctt tggcagatgt
    39721 cattatcctc agcttgccaa tcaaacaaac tattgctttc attaaggagt tggccaattt
    39781 ggatttgcga gaaggcgtta ttatttcaga tgctggttcg accaagtcaa ccattgtgga
    39841 tgcggcggag cagtatttgg ctggcaagtc tgttcgcttt gtcggggccc atcccatggc
    39901 tggtagtcac aagacagggg ctgcttcggc agatgtcaat ctttttgaaa atgcctatta
    39961 tatctttaca ccttcaagcc tgacaagtca ggacacgctt aaggaaatga aggatctgct
    40021 ttcaggtctt catgctcgtt ttatcgagat tgatgccaag gagcatgatc gtgtcacttc
    40081 tcagattagc cattttcctc atattttggc ttctagtctc atggagcaga ctgcggtcta
    40141 tgctcaagag catgagatgg caaggcgctt tgcggcaggt ggttttcgag atatgacccg
    40201 aattgcggaa agcgagccag gaatgtggac ctccattctc ttgtccaata gcgagaccat
    40261 tctggataga attgaggatt tcaaggaacg tttggaagtg attggtcagg ccattagtaa
    40321 gggagatgaa gagcaaattt ggaacttttt taaccaagcg cgtgaacaac gccagaccat
    40381 ggaaatccat aagcgtggtg gtgtggatag ctcttacgac ctctatgttg acgttcctga
    40441 tgaagaagat gtcatcctgc ggattttgga actgctacgt ggaacttcct tggttaacat
    40501 ccacatcaac gaggaaaatc gtgaggatat tcacggaatc ctacaaattt catttaaaaa
    40561 tgctcaagac ttggaaagag ctgagcatct cataacagaa aataccgact atacagtcgt
    40621 catcaaataa ggagaagaac atgtcaaata tttacgatag tgcaaacgaa ctcagtcgcg
    40681 gtctacgcgg attaccagaa tacaaggctg ttaaagcagc taaagatgcg attgcagcag
    40741 atgctgaggc aagcaaaatc tttacagaat atcttgcctt tcaagaggaa attcaaaaac
    40801 tagcacagac aggtcaaatg ccagatgcgt ccttccaagc taagatggaa ggctttggta
    40861 aacagattca aggaaatagc ctcttgtcag aattctttac caagcaacaa caattggcca
    40921 tttacctttc tgacattgaa aaaattgttt ttgaaccagt ttcagaattg ctgaaataag
    40981 aaaagaacga tcctaaagtc aggaattttt aaggaatttc tgacttttta tgataaaata
    41041 ggtaaaagta ttgcaagtat gaggtccagt atgaaactaa aaacaaacat tcgccattta
    41101 catggtagta tccgcgtccc aggtgacaag tctatcagcc accgttccat tatctttgga
    41161 agtttggctg agggtgagac caaggtttat gatattctgc gaggtgaaga cgttctttcg
    41221 accatgcagg tttttcgtga ccttggtgtt gaaattgagg ataaagatgg ggttattacc
    41281 attcaaggtg taggcatggc tggcttaaaa gcgccacaaa atgcccttaa tatgggaaat
    41341 tctggcacct cgattcgcct gatttcaggt gtccttgctg gtgcagattt cgaagtagag
    41401 atgtttggag atgatagtct ttccaaacgt cctatggacc gtgtgaccct tccactgaaa
    41461 aaaatgggcg tcagcatttc agggcaaact gaacgagact tgcctcccct tcgcttaaaa
    41521 gggacgaaaa acctaagacc tattcattat gagttgccaa ttgcctctgc ccaagtcaag
    41581 tcagccttga tgtttgcagc cttacaagct aagggggagt cagttattat cgaaaaagag
    41641 tacacccgta atcatactga agatatgttg caacaatttg gtggtcattt aagtgtggat
    41701 ggtaagaaaa tcacagtcca ggggccacaa aaattgacag gacagaaggt ggtcgtacca
    41761 ggagatattt ccagtgcagc cttttggtta gtcgcaggtt tgattgctcc aaattctcgt
    41821 ctagtgctgc agaatgtggg gatcaacgaa actcgcaccg gtattattga tgtcattcgt
    41881 gccatgggtg gaaaattgga aataactgaa atcgatccag tcgctaaatc tgctaccttg
    41941 attgttgagt cttctgactt gaaaggaaca gagattggtg gcgctttgat tccacgattg
    42001 attgatgaat tgcccattat tgccctgctt gcgactcaag cccaaggtgt aacagttatc
    42061 aaggatgctg aggagctcaa ggtcaaggaa acagaccgta ttcaggttgt ggcagacgcc
    42121 ttaaatagta tgggagcaga tattactcct acggcagatg ggatgattat caaagggaaa
    42181 tcagctcttc acggtgctag agtcaatacg tttggtgacc accgtatcgg catgatgaca
    42241 gctatcgcag ccctcttggt tgcagatgga gaggtggagc ttgaccgtgc agaagccatc
    42301 aataccagct atcctagttt ctttgatgat ttggagagct tgattcatgg ctaaggtgtt
    42361 attagggttt atgggggctg gaaaatcgac tattgcaaga ggcttggacc ctaattacct
    42421 tgatatggat gctctgattg agaagcgcct aggtatgtcc attgcgaatt ttttcgctga
    42481 aaagggagaa gcagcctttc gtcatgtaga atcagaagtc ctagctgatt tactacaaac
    42541 agaccaagtc gtgtcaactg gaggaggagt ggttatttct cagagaaatc gtgacttact
    42601 caagactaat acagataaca tctacctgaa agcagatttt gaaaccctct acctacgtat
    42661 cgcagctgat aaggacaatc agcgaccgct ttttctaaat aatagcaagg aagaactagt
    42721 agctattttt caagaaagac aggtttggta tgaggaagtg gctagtcggg ttttggatgt
    42781 gaccaagcta agcccagagg aaattataga ggaactaaga tgaaaatagc ttatctaggt
    42841 cccaagggat cattttcaca ccacgttgtg cagacagctt ttcctcatga ggaattgcag
    42901 gcttttgcca acattacaga tgtcatcaag gcttatgagc aaggattagt ggactattct
    42961 gtggtgccag ttgaaaattc tattgagggt agtgttcatg aaaccttgga ctatcttttt
    43021 catcaggctc acatccaagc agtagcagaa atcgttcagc ctattcatca gcagttgatg
    43081 gtggttccag gccatactaa gattgaaaag attttttcac atccacaggc cttggctcaa
    43141 ggaaagaaat tcatcgatga acaatatcca gaggctcaaa tcgaggtaac agctagtaca
    43201 gcttatgcgg cccgttttat ttccgaacat ccagaccagc cctttgcagc agttgcacct
    43261 agaagttctg ctgaagaata tggattggaa ctgattgccg aggatattca ggaaatggaa
    43321 gccaatttca cacgtttctg ggttctagga gctgaaaagc ctagtattcc cttgcaagca
    43381 caaactgaaa agatgagttt ggccttgaca ttacctgaca accttccagg tgcactttat
    43441 aaggccctgt cgacctttgc ttggcgagga attgacttga caaaaattga aagtcgtcca
    43501 ctcaagacag cactgggtga atactttttc attatcgatg tggattatat cgataaggac
    43561 ttggtccact ttgcccaaaa agaattagaa gcgattggaa tccagtataa aattctgggt
    43621 gcctatccta tttatccaat atcagaccat ggaaaggaga gaagatgagt aaagaaaatc
    43681 ccttaagtca tcatgagcag ttgcgttatg attatttgct aaaaaatatt cactatctca
    43741 atgagagaga aaaaaatgag tttgcctatt tgcaagaaaa gctaactctt gctaggggaa
    43801 atagtagctc tagcttggaa caagaaagag aagagcaggt tgacttacca agctatgcga
    43861 accggagtcg ctcacaatcc aaatcacaag cactctcttt ccctccaaaa aagaaaagac
    43921 ggaagctccg tcttaaacga atttttatgg tgattttttc acttttggtc tgtgtggctt
    43981 tggccatggt attcatgttt ttgcgtggtt accaagatgc tagcgcaaag aaaactgctg
    44041 atgcccgggc agctcaagta gaagtcttta atggtcagga cactagagat ggagttaata
    44101 ttttaatcat gggtactgat ggtcgaatcg gccagaacag tgttgagaca cgaactgact
    44161 ctattatggt attaaatgtc gggggctcag ataagaaaat gaagctagtc agtttcatgc
    44221 gtgataattt ggtctatata gatggttata gtcaagtgat taatggtaga aaacagacag
    44281 ataacaagtt aaacgtagcc tacgagttag gagaacaaga ggggcaaaaa ggggcagaaa
    44341 tggttcgcca agtcttgaaa gataattttg acttggacat taagtactac gccttggttg
    44401 atttccaggc ctttgccaca gcgattgaca cgcttttccc tgatggggtg acgattgatg
    44461 ctcaattttc aacattgaat gggcgtcccc taacagaagc tacagtcgga gatgatttat
    44521 acgctaccga gactgagtct ccaacccaaa ccattaaagt cggaaaacag cagatgaatg
    44581 gctcgaccct gctcaattat gctcgtttcc gtgatgatga tgaggcggat tacggccgta
    44641 caaaaagaca gcaacaagtt ttaacagcaa ttttagagca aattaaagat cccactaaac
    44701 ttttcactgg ttctgaagct cttggaaagg tttttgctat gacctcaacc aacgtaccct
    44761 atactttcct cctaacaaac ggtttatctg ttttggatgg agcaaaaaat ggtattgaaa
    44821 aattgacgat tccagaactg ggtgactggg tagatgccta tgatgtttat ggaggcttgg
    44881 gcttgctggt tgatcaaaac aaatatcaaa ccaagctcgc tcaaatgggc ttgagataag
    44941 ataatactca atgaaaatta aagtgcaaac taggaagcta gccgcaggct gctaaaaaca
    45001 cagttttgag gttgtagata agactgacga agtcagttac ctatacctac ggcaaggtga
    45061 agctgacgtg gtttgaagag attttcgaag agtataagac agaaaaatca aagtttgata
    45121 gctatttgga tagcagtcag gctttgattt tttacagtct gcaatagatt ttcacccctt
    45181 gtttgtggta taatgaaacg atacgataga aagaataatg aacaatatga ctgatttaaa
    45241 agcaattcag gctcgtagtc tggagatggc tgaatatttt gtggcctttt gtaaagaaca
    45301 tgatttgctc tgttatctct gtggcggagg cgctattggt gcccttcgaa acaagggatt
    45361 tattccttgg gacgacgacc tagacttttt tatgcctcgt aaagattatg agaaattagc
    45421 agaattatgg cctcgttatg cagatgaacg ttatttcttg tcaaagagtc acaaggattt
    45481 tgttgatcgt aatcttttta ttaccattcg tgacaaggaa accacctgta tcaagcctta
    45541 tcagcaggat ttagatttgc cacatggtct gaccttggat gttttgcctt tggattatta
    45601 tccgaaaaat ccagctgagc ggaaaaaaca ggttcgttgg gccttgattt attcactctt
    45661 ttgtgcgcaa actattccag aaaagcatgg tgctctcatg aaatggggaa gtcgcatttt
    45721 actgggtttg actccaaaat ctctccgtta tcgcatctgg aaaaaagctg agaaagaaat
    45781 gactaagtat gatttggctg attgtgatgg cattacagaa ttatgctcag gtcctggcta
    45841 catgagaaac aagtacccaa tcacatcttt tgaagacaat cttttcttgc catttgaagg
    45901 aacagagatg cctattccaa tcggctatga tgtctatctc agaactgctt ttggggatta
    45961 tatgacgcct ccaccagcag acaagcaggt accgcatcat gatgctgtca tcgctgatat
    46021 ggataagtct tatacagaat acaagggaga atatggtggc taagaaaaaa atcttatttt
    46081 ttatgtggtc tttttctctt ggaggtggtg cagagaagat tctatcaacc attgtttcaa
    46141 atctggatct agaaaagtat gatattgata ttcttgaaat ggagcacttt gacaagggat
    46201 atgaatctgt tccaaagcat gtacgcattt taaaatccct tcaagattat cgccaaacca
    46261 gatggttacg agcttttttg tggagaatga gaatttattt tccaagactg actcgtcgtt
    46321 tgcttgtaaa agatgattat gatgttgaag tttcttttac cattatgaat ccaccactgt
    46381 tgttctctaa aagaagagaa gtcaagaaga tatcttggat tcatggaagt attgaagaac
    46441 ttcttaagga tagctctaaa agagaatcac atagaagcca gttggatgct gcgaatacaa
    46501 ttgtagggat ttcaaaaaag accagcaatt ctatcaagga agtttatcca gattatgctt
    46561 ctaaattaca gataatctac aatggatatg attttcagac tattctagaa aaatctcaag
    46621 agaagatcga tatcgagatt gctcctcaaa gtatctgtac tatcggacgg attgaggaaa
    46681 ataagggttc tgaccgtgta gtggaagtga tacgattatt acaccaagag ggaaaaaact
    46741 atcatctcta ttttatcggg gctggtgata tggaagagga actgaaaaaa cgagtcaaag
    46801 agtatgggat tgaggactat gtacatttcc ttggttatca aaaaaatcct tatcagtatt
    46861 tatctcagac gaaagttctc ttgtctatgt ctaaacaaga aggttttcct ggagtgtatg
    46921 tggaggcctt gagtctggga ctccctttta tctctacgga cgttggaggg gctgaggaat
    46981 tatcccaaga aggacgattt ggacaaatca ttgagagcaa tcaagaggca gctcaggcga
    47041 ttactaatta catgacttct gcctcaaact ttgatgtcga tgaggctagc caattcattc
    47101 aacaatttac aattacaaaa caaatcgaac aagtagaaaa actattagag gagtagcatg
    47161 gaaactgcat taattagtgt gattgtgcca gtctataatg tggcgcagta cctagaaaaa
    47221 tcgatagctt ccattcagaa gcagatctat caaaatctgg aaattattct tgttgatgat
    47281 ggtgcaacag atgaaagtgg tcgcttgtgt gactcaatcg ctgaacaaga tgacagggtg
    47341 tcagtgcttc ataaaaagaa cgaaggattg tcgcaagcac gaaatgatgg gatgaagcag
    47401 gctcacgggg attatctgat ttttattgac tcagatgatt atatccatcc agaaatgatt
    47461 cagagcttat atgagcaatt agttcaagaa gatgcggatg tttcaagctg tggtgtcatg
    47521 aatgtctatg ctaatgatga aagcccacag tcagccaatc aggatgacta ttttgtctgt
    47581 gattctcaaa catttctaaa ggaatacctc ataggtgaaa aaatacctgg gacgatttgc
    47641 aataagctaa tcaagagaca gattgcaact gccctatcct ttcctaaggg gttgatttac
    47701 gaagatgcct attaccattt tgatttaatc aagttggcca agaagtatgt ggttaatact
    47761 aaaccctatt attactattt ccatagaggg gatagtatta cgaccaaacc ctatgcagag
    47821 aaggatttag cctatattga tatctaccaa aagttttata atgaagttgt gaaaaactat
    47881 cctgacttga aagaggtcgc ttttttcaga ttggcctatg cccacttctt tattctggat
    47941 aagatgttgc tagatgatca gtataaacag tttgaagcct attctcagat tcatcgtttt
    48001 ttaaaaggcc atgcctttgc tatttctagg aatccaattt tccgtaaggg gagaagaatt
    48061 agtgctttgg ccctattcat aaatatttcc ttatatcgat tcttattact gaaaaatatt
    48121 gaaaaatcta aaaaattaca ttagaacggg ggtgaccaag tgattgatgg gaaacgatta
    48181 ttatttagtt tgaccatagt cagttatgcc ttgacgctag taagtggaat tgtgtatctg
    48241 tttaataata ataatgttag cttactttct actttattgt tcttgttggt tagtagctta
    48301 attgcttgtt ggaatgatat caagtattac ttaatccatt ttattttcta tttaaccatt
    48361 tttgtatttc tggtatcaag accgaccatt gattatttta gggatggtgc tttggatacc
    48421 tatcatccaa tagcctatcg ttttgccttt atagttgtca tggtttcgat tctgggcttg
    48481 accacaggag gcattctggc tcgttacttc atagctagga agaaaataaa agtagcaaat
    48541 ataggaaatt ctctaaaaga ggtttatatc aagcggttac gctttgtatc actaggagtt
    48601 tttcttctaa cttatccttt ctatttcatt cggttatttg aacggctctt gtatcgtttg
    48661 cagacttcct actatgccta ctatgcaaat tttgaaagta aactgcctta ttttacctac
    48721 attttgtcta cctttacggt ctatgcaatg tgtatgtatc tggcaaccaa gccaaagaaa
    48781 ttgcaggcca cagcagtgct tgtctccttt attgcagcta atactattca tttggcaatt
    48841 gggacacgaa atccctttat tttaagtatt ttatttgctt ttgtttatta ctttatgcgg
    48901 gagcaaactg aaaaaggaaa atggattggg tttaaagaaa agttagcgat ttttgtaggt
    48961 tctcctattc tcatgttagc gatgggagta ctcaattatg tacgggataa tgtccaagtt
    49021 tcccatacag gtttctggga tatcttactt gactttatct ataaacaagg gactagtttt
    49081 ggtgttctgg ctcgaggttt tctatttaac agtagcctcc cttaccgaga tttccgtaat
    49141 tttacttttg gtcctgttct tgattatttt gcaaggggga gtttgggagc cattttcgga
    49201 ggaaaagcct ttgaacatac aaccaatagt gtggaactag ctattgatag taatagttat
    49261 gcccacaatc tatcctatct tgtcttgaac aaggaatacc tgaaagggca tggtatcgga
    49321 agtagttata tcatggagtt gtataccgac tatggtatga ttggagtctt tctgcttagt
    49381 ttcttactcg gcgtattatt tatagccatg ctgcaagtag cctatcgctc aaggacaatc
    49441 ctatttgctt tatccctact catcttgaat aatctattct ttatgccaag aagcagcttt
    49501 tcagaaagtt tcttcaattt atttacaatg caattctggg gaattgttct tgtgattata
    49561 tttatagcaa aaatgcttac aaaggaaaac tagtatctac taaacaaagg agaaaaaaat
    49621 catgtttgaa cattattcag tagctgattt gtttgcaaat ctttataaaa agcgaaaagc
    49681 aaatatttta gctctcatcg ctttatttgc tctcattgct gtaccattta caattaaagc
    49741 agttaggaat aaaaacactg tcaaagatac aacaagttat tcaacttatc ttatctataa
    49801 aatcactcct ccaaaagagt cggccaaaac gattttgaat catcaaattg gtggttatag
    49861 tgatttttat gggaaattga ttgatggtaa tttgaatgga gcttatcttt tcaatgatgt
    49921 agaacccagt gagttgaaaa aaattgccag tgaattagac acgacagaaa caaccttgaa
    49981 aaattcaacg aatgactatt ggtggaaaaa attgaccgtc tactatatga ttgacgatgc
    50041 aggggttggt gtgaaaattt tgacatcaag taaagatgcc aatgacttgt tagagaaaaa
    50101 aattgatggg ttgattgaga aatttaaaca tgcctatgca aatgtgaaaa ttgaaaaact
    50161 ggaaaccatc aactctaaag aattgaacgc aaatggtgaa acagcgcttg gcttaaatgt
    50221 gaaaaatctg attcttcgtt tagttgttat tggagtggtt tgtgtgattt tggttgtgat
    50281 gggaaatgtg ttagtttatc tctttaatcc aacaatcaat agagtaggtg atttttctca
    50341 gtatcaaatt gattttgtaa cagagattac aacaattgct aacctagcag atgttttgtc
    50401 atacaaaaat actggacagg aattgaccat cgttagctca aataaagcta tcctagataa
    50461 attgaaacag agtcaagaag ctttaaaagg aatgcatttt gtagatttac aggatgtatc
    50521 atctcttttg gaaagagata cagtccttct tgttgaagag tacggagtga ctcgttataa
    50581 gaaatttgag caaagtcttc aaattctcag aaacttaaat cgttctatcc tcggtgtagc
    50641 aacctttaaa ttataagtat tctgatgtat aaggtcttca aaatcggctc ttagccgatt
    50701 ttgaaggctt tttctatctg tctgcagaaa ttttctcttg gagtctccta tagaaagacc
    50761 ctagggaaaa tctatccgta tagaggagcc ttcttttgat aaaatcgtaa aaatctagta
    50821 taatagatag aactgaaagt atgaggttac tagcaatgaa aatgaaacaa attagtgata
    50881 caactttaaa aatcacgatg tctttagagg atttgatgga tcgtggaatg gagattgctg
    50941 actttctcgt tcctcaagaa aaaacagaag agttctttta tgctatcttg gatgagctag
    51001 agatgcctga tagctttctg gatacaggca tgttgagctt ccgtgtgact ccaaaacctg
    51061 ataaggtaga tgtctttgtg accaagtcaa agattgacca aaatctagat tttgaagact
    51121 tatcggattt gccagatatg gaagaattgg ctcaaatgtc tccagatgaa tttatcaaaa
    51181 ccctggaaaa aagcatcgca gacaaaacca aggatgatat cgaagccatt caatctcttg
    51241 agcaagttga agccaaggaa gaagagcaag agcaggctga acaagaagct gagagtaaga
    51301 aagaacctta catctactac atcctttctt ttgctaagtt ggctgacttg gtagcttttg
    51361 ccaagacagt gacttttgaa atggaaactt ctgaactcta caaaatgaac gagcgctatt
    51421 atttgaccat tttagtggat attgaaaatc atccaagccc atatccagct tggctgttgg
    51481 cccgtatgcg cgagtttgca gacgatagtg atatcagtcg ctcagtctta caagagtatg
    51541 gtcaagtctt gatgagtcac gatgcagtgc tcaatctgca aaaaatcggc taatcttttc
    51601 tggaaagcta ttttcagatt ttaagaagag cgaatcgtct gattcgtttt ttctttttat
    51661 actgaaatag tgatttacta taataggaat tttcacaaaa ttctgttata atggctatat
    51721 tagaaaattt cgaggagata aagatgacag ttaaaattgc tttactagga tttggtaccg
    51781 ttgcaagtgg tgtgcctttc ctcctaaagg aaaatggagg aaaaatcaat caatcagcac
    51841 attcagatat caaagttgct aaggtattgg tcaaggatga agatgaaaaa aatcgcttgc
    51901 ttgcagcagg gaatgacttt aactttgtaa ccaatgtgga tgatatttta tcagaccagg
    51961 atattactat cgtagtggaa ttgatggggc gtattgagcc tgctaaaacc tttatcactc
    52021 gtgccttgga agctggaaaa cacgttgtta ctgctaacaa ggacctttta gctgtccatg
    52081 gcgcagaatt gctagaaatc gctcaagcta acaaggtagc actttactac gaagcagcag
    52141 ttgctggtgg gattccaatt cttcgtactt tagcaaattc cttggcttct gataaaatta
    52201 cgcgcgtgct tggagtagtc aacggaactt ccaacttcat ggtgaccaag atggtggaag
    52261 aaggctggtc ttacgatgat gctcttgcgg aagcacaacg tctaggattt gcagaaagcg
    52321 atccgacgaa tgacgtagat gggattgatg cagcctacaa gatggttatt ttgagccaat
    52381 ttgcctttgg catgaagatt gcctttgatg atgtagccca caagggaatc cgcaatatca
    52441 caccagaaga cgtagctgta gctcaagagc ttggttacgt agtgaaattg gttggttcta
    52501 ttgaggaaac ttcttcaggt attgctgcag aagtgactcc aaccttccta cctaaagcgc
    52561 acccacttgc tagtgtgaat ggcgtaatga acgctgtctt tgtagaatct atcggtattg
    52621 gtgagtctat gtactacgga ccaggtgcgg gtcaaaaacc aactgcaaca agtgttgtag
    52681 ctgatattgt ccgtatcgtt cgtcgtttga atgatggtac tattggcaaa gacttcaacg
    52741 aatatagccg tgacttggtc ttggcaaatc ctgaagatgt caaagcaaac tactatttct
    52801 caatcttggc tctagactca aaaggtcagg tcttgaagtt ggctgaaatc ttcaatgctc
    52861 aagatatttc ctttaagcaa atccttcaag atggcaaaga gggtgacaag gcgcgtgtcg
    52921 ttatcatcac acacaagatt aataaagccc agcttgaaaa tgtctcagct gaattgaaga
    52981 aggtttcaga attcgacctc ttgaatacct tcaaggtgct aggagaataa gatgaagatt
    53041 attgtacctg caaccagtgc caatatcggg ccaggttttg actcggtcgg tgtagctgta
    53101 accaagtatc ttcaaattga ggtctgcgaa gaacgagatg agtggctgat tgaacaccag
    53161 attggcaaat ggattccaca tgacgagcgt aatctcttgc ttaaaatcgc tttgcaaatt
    53221 gtaccagact tgcaaccaag acgcttgaaa atgaccagtg atgtcccttt ggcgcgcggt
    53281 ttgggttctt ccagctcggt tatcgttgct gggattgaac tagccaacca actgggtcaa
    53341 ctcaacttat cagaccatga aaaattgcag ttagcgacca agattgaagg gcatcctgac
    53401 aatgtggctc cagccattta tggtaatctc gttattgcaa gttctgttga agggcaagtc
    53461 tctgctatcg tagcagactt tccagagtgt gattttctag cttacattcc aaactatgaa
    53521 ttacgtactc gcgacagccg tagtgtcttg cctaaaaaat tgtcttataa ggaagctgtt
    53581 gctgcaagtt ctatcgccaa tgtagcggtt gctgccttgt tggcaggaga catggtgacc
    53641 gctgggcaag caatcgaggg agacctcttc catgagcgct atcgtcagga cttggtaaga
    53701 gaatttgcga tgattaagca agtgaccaaa gaaaatgggg cctatgcaac ctacctttct
    53761 ggtgctgggc cgacagttat ggttctggct tctcatgaca agatgccaac aattaaggca
    53821 gaattggaaa agcaaccttt caaaggaaaa ctgcatgact tgagagttga taccaaaggt
    53881 gtccgtgtag aagcaaaata aagaatagaa gataggatgg ggaaactctt gaccagaggg
    53941 tttcatatcc tttttgtgaa aagaa
//
LOCUS       DAVBCW010000003        49431 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVBCW010000003 DAVBCW010000000
VERSION     DAVBCW010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 49431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49431
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.003"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(276..1526)
                     /gene="ilvA"
                     /locus_tag="VQ514_000131"
     CDS             complement(276..1526)
                     /gene="ilvA"
                     /locus_tag="VQ514_000131"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HET2498959.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(1576..1932)
                     /locus_tag="VQ514_000132"
     CDS             complement(1576..1932)
                     /locus_tag="VQ514_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET2498960.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEIFGDE"
     gene            complement(2170..2406)
                     /locus_tag="VQ514_000133"
     CDS             complement(2170..2406)
                     /locus_tag="VQ514_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2498961.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKY"
     gene            complement(2513..3535)
                     /gene="ilvC"
                     /locus_tag="VQ514_000134"
     CDS             complement(2513..3535)
                     /gene="ilvC"
                     /locus_tag="VQ514_000134"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET2498962.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            complement(3601..4077)
                     /gene="ilvN"
                     /locus_tag="VQ514_000135"
     CDS             complement(3601..4077)
                     /gene="ilvN"
                     /locus_tag="VQ514_000135"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HET2498963.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            complement(4070..5770)
                     /locus_tag="VQ514_000136"
     CDS             complement(4070..5770)
                     /locus_tag="VQ514_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HET2498964.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDILPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            complement(5970..7637)
                     /locus_tag="VQ514_000137"
     CDS             complement(5970..7637)
                     /locus_tag="VQ514_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HET2498965.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            complement(7640..8005)
                     /locus_tag="VQ514_000138"
     CDS             complement(7640..8005)
                     /locus_tag="VQ514_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET2498966.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            complement(8162..8350)
                     /locus_tag="VQ514_000139"
     CDS             complement(8162..8350)
                     /locus_tag="VQ514_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HET2498967.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            complement(8466..8642)
                     /locus_tag="VQ514_000140"
     CDS             complement(8466..8642)
                     /locus_tag="VQ514_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HET2498968.1"
                     /translation="MDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLIN
                     DGMWRAENVTQDLI"
     gene            complement(8753..8902)
                     /locus_tag="VQ514_000141"
     CDS             complement(8753..8902)
                     /locus_tag="VQ514_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HET2498969.1"
                     /translation="MDKVETTIKSLCVHHYNLKFYVFNDDLPREWFQLMEKRLETLNS
                     EIVNV"
     gene            complement(9214..10758)
                     /locus_tag="VQ514_000142"
     CDS             complement(9214..10758)
                     /locus_tag="VQ514_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HET2498970.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLEIFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVINAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMNPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            10971..11273
                     /gene="gatC"
                     /locus_tag="VQ514_000143"
     CDS             10971..11273
                     /gene="gatC"
                     /locus_tag="VQ514_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HET2498971.1"
                     /translation="MKITQEEVTHVANLSKLRFSEEETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDRLFKNVPEQDNYYIKVPAILDDGGDA"
     gene            11273..12739
                     /gene="gatA"
                     /locus_tag="VQ514_000144"
     CDS             11273..12739
                     /gene="gatA"
                     /locus_tag="VQ514_000144"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HET2498972.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDANNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDTKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGIDPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            12739..14181
                     /gene="gatB"
                     /locus_tag="VQ514_000145"
     CDS             12739..14181
                     /gene="gatB"
                     /locus_tag="VQ514_000145"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HET2498973.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDLHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            14310..14870
                     /gene="efp"
                     /locus_tag="VQ514_000146"
     CDS             14310..14870
                     /gene="efp"
                     /locus_tag="VQ514_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HET2498974.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            14892..15281
                     /locus_tag="VQ514_000147"
     CDS             14892..15281
                     /locus_tag="VQ514_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET2498975.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGVYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            15274..15696
                     /gene="nusB"
                     /locus_tag="VQ514_000148"
     CDS             15274..15696
                     /gene="nusB"
                     /locus_tag="VQ514_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET2498976.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTNVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKVGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(15745..16122)
                     /locus_tag="VQ514_000149"
     CDS             complement(15745..16122)
                     /locus_tag="VQ514_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2498977.1"
                     /translation="MVLSNGQPTFLSTILSFVLLVIIGPVFEETLDRGYFMNTFFPKS
                     KYYLDVILSGLIFGISHLILSHRDPISLLYYSLIGFFFALVYRSTDNLRLTILCHSFF
                     NFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(16463..16648)
                     /gene="briC"
                     /locus_tag="VQ514_000150"
     CDS             complement(16463..16648)
                     /gene="briC"
                     /locus_tag="VQ514_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HET2498978.1"
                     /translation="MTGTETFTVLSTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRTG"
     gene            complement(16807..17574)
                     /locus_tag="VQ514_000151"
     CDS             complement(16807..17574)
                     /locus_tag="VQ514_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HET2498979.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEVGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            complement(17571..18437)
                     /locus_tag="VQ514_000152"
     CDS             complement(17571..18437)
                     /locus_tag="VQ514_000152"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HET2498980.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            complement(18474..19841)
                     /gene="accC"
                     /locus_tag="VQ514_000153"
     CDS             complement(18474..19841)
                     /gene="accC"
                     /locus_tag="VQ514_000153"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET2498981.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            complement(19853..20275)
                     /gene="fabZ"
                     /locus_tag="VQ514_000154"
     CDS             complement(19853..20275)
                     /gene="fabZ"
                     /locus_tag="VQ514_000154"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HET2498982.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGTLTFAIGN"
     gene            complement(20272..20757)
                     /locus_tag="VQ514_000155"
     CDS             complement(20272..20757)
                     /locus_tag="VQ514_000155"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HET2498983.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVASEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            complement(20760..21995)
                     /gene="fabF"
                     /locus_tag="VQ514_000156"
     CDS             complement(20760..21995)
                     /gene="fabF"
                     /locus_tag="VQ514_000156"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET2498984.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            complement(22017..22748)
                     /gene="fabG"
                     /locus_tag="VQ514_000157"
     CDS             complement(22017..22748)
                     /gene="fabG"
                     /locus_tag="VQ514_000157"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HET2498985.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVIAIDGGLSM"
     gene            complement(22782..23702)
                     /gene="fabD"
                     /locus_tag="VQ514_000158"
     CDS             complement(22782..23702)
                     /gene="fabD"
                     /locus_tag="VQ514_000158"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HET2498986.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            complement(23695..24669)
                     /gene="fabK"
                     /locus_tag="VQ514_000159"
     CDS             complement(23695..24669)
                     /gene="fabK"
                     /locus_tag="VQ514_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HET2498987.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     ISIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVE
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWAGVVRND"
     gene            complement(24789..25013)
                     /locus_tag="VQ514_000160"
     CDS             complement(24789..25013)
                     /locus_tag="VQ514_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET2498988.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            complement(25073..26047)
                     /locus_tag="VQ514_000161"
     CDS             complement(25073..26047)
                     /locus_tag="VQ514_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HET2498989.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGEELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANNRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            complement(26047..26481)
                     /locus_tag="VQ514_000162"
     CDS             complement(26047..26481)
                     /locus_tag="VQ514_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET2498990.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            complement(26575..27360)
                     /locus_tag="VQ514_000163"
     CDS             complement(26575..27360)
                     /locus_tag="VQ514_000163"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HET2498991.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            27685..29049
                     /locus_tag="VQ514_000164"
     CDS             27685..29049
                     /locus_tag="VQ514_000164"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HET2498992.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            29052..29423
                     /locus_tag="VQ514_000165"
     CDS             29052..29423
                     /locus_tag="VQ514_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HET2498993.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            29637..30911
                     /gene="serS"
                     /locus_tag="VQ514_000166"
     CDS             29637..30911
                     /gene="serS"
                     /locus_tag="VQ514_000166"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HET2498994.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGTDEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSAAKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(30936..31835)
                     /locus_tag="VQ514_000167"
     CDS             complement(30936..31835)
                     /locus_tag="VQ514_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HET2498995.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACSGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(31945..32493)
                     /locus_tag="VQ514_000168"
     CDS             complement(31945..32493)
                     /locus_tag="VQ514_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET2498996.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            complement(32711..34033)
                     /locus_tag="VQ514_000169"
     CDS             complement(32711..34033)
                     /locus_tag="VQ514_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HET2498997.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSITESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMALIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGARFIWLYRVFFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(34124..34212)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(34376..36712)
                     /locus_tag="VQ514_000170"
     CDS             complement(34376..36712)
                     /locus_tag="VQ514_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HET2498998.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVHFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(36816..37364)
                     /locus_tag="VQ514_000171"
     CDS             complement(36816..37364)
                     /locus_tag="VQ514_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HET2498999.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIFYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(37361..37663)
                     /gene="zapA"
                     /locus_tag="VQ514_000172"
     CDS             complement(37361..37663)
                     /gene="zapA"
                     /locus_tag="VQ514_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HET2499000.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            37750..38631
                     /locus_tag="VQ514_000173"
     CDS             37750..38631
                     /locus_tag="VQ514_000173"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HET2499001.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQLVEENRGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TSDQHDFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSNPISLEEKAEG
                     KYLSVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLSFC
                     AKLHFKNTEKAKKRLER"
     gene            38635..39249
                     /gene="lepB"
                     /locus_tag="VQ514_000174"
     CDS             38635..39249
                     /gene="lepB"
                     /locus_tag="VQ514_000174"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET2499002.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRLWPITRIGTF"
     gene            39377..41743
                     /locus_tag="VQ514_000175"
     CDS             39377..41743
                     /locus_tag="VQ514_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HET2499003.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLAKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGKQKSHDLKTIQKHITTIEKELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDLRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSSISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSEESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKGDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITDTDLKEIFGK"
     gene            complement(41791..43074)
                     /locus_tag="VQ514_000176"
     CDS             complement(41791..43074)
                     /locus_tag="VQ514_000176"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HET2499004.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVITAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     ATAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
     gene            complement(43828..44964)
                     /locus_tag="VQ514_000177"
     CDS             complement(43828..44964)
                     /locus_tag="VQ514_000177"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HET2499005.1"
                     /translation="MKHSVHFGAGNIGRGFIGEILFKNGFHIDFVDVNNQIIHALNEK
                     GKYEIEIAQKGQSRIEVTNVAGINSKEHPEQVIEAIQKTDIITTAIGPNILPFIAELL
                     AKGIEARRVAGNTQVLDVMACENMIGGSQFLYQEVKKYLSPEGLTFADNYIGFPNAAV
                     DRIVPAQSHEDSLFVVVEPFNEWVVETKRLKNPDLRLKDVHYEEDLEPFIERKLFSVN
                     SGHATSAYIGAHYGAKTILEALQNPNIKSRIESVLAEIRSLLIAKWNFDKKELENYHK
                     VIIERFENPFIVDEVSRVARTPIRKLGYNERFIRPIRELKELSLSYKNLLKTVGYAFD
                     YRDVNDEESIRLGELLAKQSVKDVVIQVTGLDDQELIDQIVEYI"
     gene            complement(45026..45463)
                     /locus_tag="VQ514_000178"
     CDS             complement(45026..45463)
                     /locus_tag="VQ514_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2499006.1"
                     /translation="MKLEKHLIKLNKQFSNKEEAICYCGQVLYEGGYVNEDYIEAMIE
                     RDKELSVYMGNFIAIPHGTDAAKKDVLKSGITVVQVPRGVDFGNVSNPQVATVLFGIA
                     GIGNEHLEIIQKISIFCADVDNVLKLADAQSKEEVLRLFDAVE"
     gene            complement(45465..47420)
                     /locus_tag="VQ514_000179"
     CDS             complement(45465..47420)
                     /locus_tag="VQ514_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HET2499007.1"
                     /translation="MLLTKREEQLLKAFLHVGKLSMQDMTEILQVSSRTIYRTLSDLT
                     DSMEQYGIEITKHGKYYILTGELDDLPTELEVLVEYSPQERQELITYRLLTESGFVTN
                     EALQECTKVSNVTIIQDISDIDKRLLDFDLKIERQKGYRISGDSVGKRRFLAILLTNC
                     ISVADFSTGNFGSFDILEADRTRLASQIVNKQLSGFPDMDARMKMFFAILLSLIGQEQ
                     NIENSPNTSKQALEISQKIFQTYSKQTAQFYSIQEIIYFASILDELIIKRQDNPLFTE
                     KFDGEFFYNISNLIDTVSMYTKIDFFKDKVLFNFLFHHIRLSLGVPILFQGENLPESV
                     QILVERNKFLYTVISLLVNDIFPKYLHTEYEYGMIALHFISSLGRSPEIYPVRVLLLT
                     DERRVTRDLLVSKIKSVAPFVELIDIQSLVDYHSIDLSQYDYILSTKPLTNQEIDVIS
                     SFPTIKELLELQERLQYVQAHRTIVARDAIAPEKSYDLQDYLISSSQLLSQFELVQLE
                     NNQSFEHTVEQIIQYQKNVSDRDYLTRKLLSHFQNSPMAIPNTGLVLLHSQSSKVTTN
                     SFTMFELKLPISALSMKREEEEVKRCLLMLMSKEASEEARDLMTAISQSIIENHLYTE
                     IYKTGNQSIIYQMLNTIFNEKIKKLEN"
     gene            complement(47444..49213)
                     /locus_tag="VQ514_000180"
     CDS             complement(47444..49213)
                     /locus_tag="VQ514_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HET2499008.1"
                     /translation="MEEKVSLKVRVQKLGTSLSNMVMPNIGAFIAWGVLTALFIADGY
                     LPNEQLATVVGPMLTYLLPILIGYTGGYMIHGQRGAVVGAIATVGAITGSSVPMFIGA
                     MVMGPLGGWTIKKFDEKFQEKIRPGFEMLVNNFSAGLVGFALLLLAFYAIGPVVSTLT
                     GAVGNGVEAIVNARLLPMANIIIEPAKVLFLNNALNHGIFTPLGVEQVAQAGKSILFL
                     LEANPGPGLGILLAYAVFGKGSAKSSSWGAMVIHFFGGIHEIYFPYVMMKPTLFLAAM
                     AGGISGTFTFQLLDAGLKSPASPGSIIAIMATAPKGVWPHLNVLLGVLVAAVVSFLVA
                     ALILHADKSTEDSLEAAQAATQAAKAQSKGQLVSTSVDAVVSTDSVEKIIFACDAGMG
                     SSAMGASILRDKVKKAGLEIPVSNQAISNLLDTPKTLIVTQEELTPRAKDKSPSAIHV
                     SVDNFLASPRYDEIVASLTGAFPIAEIEGDIPTSAPVDSQEIDLNHIDAVVVAYGKAQ
                     GTATMGCETIRAIFRNKNIRIPVSTAKISELGEFNSKNIMIVTTISLQAEVQQAAPNS
                     QFLIVDSLVTTPEYDKMAARMYK"
BASE COUNT        14542 a        11190 c         9258 g        14441 t
ORIGIN      
        1 tgctcaaaac actgttttga ggttgcagat agagctaacg tggtttgaag agattttcga
       61 agagtattaa agacagtgtg actatgcttg tcaatagata ggaaatcaaa atgaaggatg
      121 gaaatctgag ccaaacatca taagtgctat aatgaacaga agtaagaatt gcacgataag
      181 ttttctaaaa ccataggaat cgtccccttt cttcatcttc attatagcat atatacatca
      241 gttgtacaaa taatatgata tttttttatt agtcctcaga caagcatatt ataaagcgtt
      301 tcattaccat ttaagttaat ataagctgga tcaaaacctt ccattctacg aatcaaacct
      361 gcataatcat gcttatctgc taaagcgatc ccaatcaata ctgggcctgt tcccttgcta
      421 gctcgtttga tatactcaaa acgtgtgata tcatcatttg gccccaggat atcatttaca
      481 aactcacgca aagctcctgg acgttgtggg aaattgacca caaaataatg tttgatacca
      541 tcataaatca aggcacgctc ttccatttct ggcatacggt tgatatcatt atttcctcca
      601 gaaatgatac aacaaatggt tttcccctta atatattcag ctaaaacctc taaagaggcg
      661 atactagctg ctccagcagg ttctgcgact atcccttgct tagagtaaag gtcaatcaag
      721 gtttcagaaa tcaatccctc atcgacacct accaaagttt gaatatgttg acgagttgct
      781 tcataggtca actgacctac cttttgcaca gcaatcccat ccgcaaattt atcaatttcc
      841 ttgagtttga ctggacctcc agcctcaaag gcagctttca tggaacgcgc tccattcgcc
      901 tctactccga taacctcaat ctctggactt gtttccttga tataggtaga aaccccggca
      961 atgagaccgc caccaccaac aggaaccaag acagcatcaa aatcaatcga ttcttttcga
     1021 gcttcttcta aaatctcata agcaactgtt ccttgacctg cttgaacatg agcatcatca
     1081 aaaggatcaa taaaggtacg attttcagag actgtaaatt cttgagctgc tttggctgag
     1141 gcatcaaagg tatctccaac tagtttaata gttacaaaat ccccaccaaa aaagcgaacc
     1201 tgaccaatct tttgttgtgg cgtagtaata ggcataaaga tagtagcagg aattttcatt
     1261 tcattacaag tataggctac tccctgcgca tgatttcccg cagaagcgca aactacccca
     1321 cgttcacgtt cttccttgct gagctgggaa atggcataat aggcaccacg aattttaaag
     1381 gagcgaacac gctgggcatt ttcttttttc aaataaatct tagcaccata cttctccgat
     1441 aaataatgat cgtaatccag tggagtattc acaaccacac cgttcaagac cttgtgagcc
     1501 ttgattatat cttttgaact taacatataa aacctctttt tctataaaaa acagtcacta
     1561 atgactgttt tttagttatt catctccgaa aatttcagta acactttcat ggtatttatg
     1621 agccatacga atcgaaaact ctttaggaag ttcataacga tttgtggaaa tccaatcata
     1681 taaccattct accaaacttt cttcactatc attacgatgg aagataaaat agtttaaaat
     1741 ttctttatat tctacatcat tcaaatccaa cgtgtctaca aattgactac gaggaacaaa
     1801 attagaatct aattttctta gttgatctgt caattctccc aattgtaaat ccaactgaaa
     1861 aaatagtttt tgcagttgcc tatcagtttc atcacatcct gtcttgatag aaaacaaatt
     1921 ggtaatattc atatgatttc aacccctaaa taagtacatc taatatccca cgaatagctg
     1981 aagctaaagt tttcgcagtc cagtcattta aacccgattc tttcaatcca gatgcaataa
     2041 tttgctctat ttgatataaa ccgccatcta tcgtctcatt tattctgcga agaggacctt
     2101 caatatagga taaagcttct ccgaatctaa cagctgattt aggtcctaat gatttaccaa
     2161 ctatatttgt taatattttt tatttctcaa aatccatttc gctatataac gagcagattt
     2221 agtcgcacca gctctctcaa acggagaatg aatttcaaga ccttcttctc tagctatttg
     2281 tgccaatttg aagaattgtt caaaggtcat atgcaaccta ccatcttcaa aattagcatc
     2341 atcataaatg ttaattacag cattttcatc atacgaccaa tctgctttat tttggtctgc
     2401 tagcactatt accgacggtg taattacatt atacaactag aaccaataat aacgatgcaa
     2461 tttttctcat aatctttttc ataaaatcat ctccagcgct atatatttct aattagttat
     2521 agattttgaa tgcatcatcg tcgtttttac caacgaatgg cattgcttta cgcaattctg
     2581 caccaacttt ttcaatttca aggttagctg cttgttcacg gtaagcagtc aattttggac
     2641 gtccagcttt atagtcattt acaaagtcat ttgcaaattt accattttgg atgtctgcca
     2701 agacagcctt catattttct ttaacttgtt cagtgattac acgtggacct gatacatagt
     2761 caccgtattc agcagtgttt gaaatagatt gacgcatttt cttgaatcca ccttcgtaga
     2821 tcaagtcaac gatcaatttc atttcgtgaa gaacttcaaa gtaagccaat tctggagcgt
     2881 aacctgcttc tgtcaagact tcgaaacctg cttcgataag ggcagtcaaa ccaccacaaa
     2941 gtacagcttg ttcaccaaac aaatcttctt cagtttcttc tttgtaagtt gtttcaagaa
     3001 gacctacacg agccgctcca acacctttac accagtccat agcaatgttt ttagcatttc
     3061 ctgttgcatc ttggtatact gcataaagag ctggaacacc aaatccttct tcgtaagtac
     3121 gacgtaccaa gtgtcctggt cctttaggag cacacatgaa gacatctaca tccgcaggaa
     3181 ctttgataaa ttcaaagtgg atgttgaaac catgggcaaa tccaactgcg tttccagctt
     3241 ccaagtttgg agcgatttct gcttcgtaca attcttgttg aatttcgtct ggcgccaaga
     3301 tcatgataac atcagccaac ttagtagctt ctgctactgt gtaagtatca aatccatctt
     3361 cttttgcttt atcaaaagat ttacctggac gtacaccgat aataacgtca cgacctgaat
     3421 cacgcaagtt ttgagcatgc gcatgccctt gtgaaccata accgataacg gcgatttttt
     3481 taccgtcaag tgctgctact ttaacatctt tttcatattc catttgaact gtcatagctt
     3541 ttctctcttt tctattattt attgcctttt aggctggttt aacaaattta agttggattt
     3601 ttaatcgcgg gtaaatccag ttgcacccgt tcgagcaata ttgcgaatac cgtatgggcg
     3661 aatgactctc aatagggctt cgctcttttc tgcatttccc gtcatctgaa tggtaatcga
     3721 gcttggcgct acgtctacca ctgttgcacg gaaaggttga ataatcgcta aaatctcagc
     3781 tctcttctca gctggcgctg acatcttaac caaaatcacc tcgcgctcca aatgaggctt
     3841 gtctgtaata tctcgaatgc gaatcacatc aatctgacga ttgagctgtt tgatgatttg
     3901 ctccacttca tcatgagaag caacatcaat aataatagtg atacgcgata cattcggatc
     3961 ttctgttgct ccaacagaga tgctttcaat attaacctga cgacgagata ggacacctgt
     4021 aaagcgattg aggactcctg aacgattttg tagttttgct gttaacattc tacgcatgga
     4081 actgcacccc caacatctca tgattactct taccagccgg taccattggt aacacctgtt
     4141 ccttacgaga aatatctacc tcaattagca taggaacatc ctcagtgatg acttcaaggt
     4201 cttgagccaa ggtctcagga ttgtcaaact tatagttttt aataccataa gcctgcgcca
     4261 tcaattggaa atcaggaagg atatcaaaga ccgactctga tgttctgcct tcatagaagg
     4321 attcctgcca ctggcgaacc attccgagtg aatgattgtt cagcataacc accttgattg
     4381 gcaccttgta aatattcaaa atagccaact cctggttagt catttggaaa ccaccatccc
     4441 caacaaacaa gactacttcc ttatctgggt tagcaatttt agcaccgatt gctgctggaa
     4501 ttccaaagcc cattgttccc aaaccacctg aagtcactaa ctgacgttca ttttggtagg
     4561 gataatactg agctgtccac atttggtgtt gaccaacgtc tgttaccaca atggcatctc
     4621 cattcgtcaa ttcaccaatt cgttcaataa ctgcttgcgg ttgaaccaca cgctctttct
     4681 tgtcataaga acgaacacga ttcttgtctt tagtgacttt ctcaatccac ttttcagtat
     4741 tgttgtgaac tgttggttct gctagcaaca tttgcaaggc cttcttagca tctccaacta
     4801 caggaatgtc tgcactgata atcttgccaa tctcagctgg gtcaatatca atgtgggcaa
     4861 ccttagcatt cttagcgaaa gtcttaggat tccccgtcaa acggtcatcg aaacgagaac
     4921 caatactaat cataaagtcc gcttccgtca tggcaatatt tgctgcgaat gacccgtgca
     4981 tgcctcccat tccaagaaag agtgggtgac tcgttgcaat cgttccttgt cccaaaagac
     5041 tggttaccac tggaatttga tagcgttctg caaattcatt tagttccgta gcagcctcag
     5101 cataactaat tccaccacca gctaacaaga ctggcttttt agccttggac aattgcttca
     5161 agattttctt gatttgcata tcattcggct caagagtcgg ctgataactt ggtaaattca
     5221 cttctggtga ataaatgaag tctgtttcta aagcagatat gtcttttggt aggtcaatta
     5281 caactggccc tggacggcct gtagttgcga tatggacagc ttccgtaatg atacgcggaa
     5341 tatcagctgt ctcacgaact tggtaattgt acttagtgat tggcatggta attcccacga
     5401 tgtctgcctc ctgaaaggca tccttcccaa tccctgctcg cgccacctga cctgtaaaga
     5461 ccaaaagggg aacgctatcg ctcatggcat ccgcaatccc tgtaatggca tttgttgctc
     5521 ctggtccact agtgacgacg gcaacaccca actttccagt tgatttggca taaccttcag
     5581 cttcatgcaa acaaccttgc tcatggcgcc ctagaatgtg gcgaatgcct ttaaaattat
     5641 atatcgcatc ataaaaaggc aaaaccgcac caccaggata accaaagatg gtatcaactc
     5701 ctaaatcacg aagtgtttcc aaaactaggt ccgaccccgt cttaggagat tctaaactga
     5761 ttttctccat tattcccctt tctttcctct taaaaataac ttgttactat cataccattt
     5821 tttcaaaatt tttcaagata aaagaagaaa ttttctgaat tttctatttt aacgtttatt
     5881 tatgaatgtt atttttgttt ttattaaaaa gataccgatt tcatttacta aaaagaaaaa
     5941 agaactgatt tctcagtcct tcattaatct tattccacac taaataggta tgggtaaaca
     6001 ggttgttgac cttggtgaat ctcgacttca acgtcttcga attcttctac gatttcttga
     6061 gcgatttcat tggcaagttc ttcgcttccg tcttcaccga catagaaggt tacgatttca
     6121 ctatcttcat ccaacatatg tttcaaggtt tcagtcaatg tttggtgcat atcagggttt
     6181 gacacaagaa tttttccatc caccatacct agattatcgt tttcatggat ttctaagcca
     6241 tcgatcgttg tatcacgcac ggctgttgtg acgcttccgc taacgacatc gctaagagca
     6301 gctgtcatac gctcttggtt ttcttcaatg gacttgcttg gatcaaaggc aagaagactt
     6361 gtcatacctt gaggaagagt gcgagcctct accactactg ctggttgctc caaaacttct
     6421 gccgcagatt gagctgccat gaagatgttc ttgttgtttg gcaagaagat gatgttacgg
     6481 gcattgacct gttcaacagc cttgataaag tcttctgttg aagggttcat ggtttgaccg
     6541 ccttcgataa cataatccac gccttgagaa cagaagatat ctgctagacc tttaccagcc
     6601 accacagcaa tcaaagcata ctctttttct tcagccgact tgataacttg agtagcttct
     6661 ttctcaacct gtgcttcgtg ttggttacgc atattgtcaa cttttacctt gaccaagcta
     6721 ccatatttga gaccttcttg cataacaagt cctggatctt ctgtatgaac atggactttg
     6781 acaatttcat catcgttaac aacgaggaga gaatccccaa gctcatccaa gtagttacgg
     6841 aattcatcgt agtcaaaatc tttagcatag gttggacctt gcttaagagc taccatgatt
     6901 tcagtacagt aaccaaacgt gatgtcctca gttgctacgt gaccagctac agacttatga
     6961 tgctctacat tgatcatctc actcatgttg gcaggagtcg ctacaaagtc ctcagatgca
     7021 atatattcgc cagtaagggc tgaaaggaaa ccttcgtaga tgaagaccaa tccttgacca
     7081 cctgagtcca caacgccaac ttctttcaat actggaagca tgtctggtgt tttagctaga
     7141 gctgttttag caccttccaa ggctgcgcgc atgacttcaa cagtgtcatc tgtttgctca
     7201 gcttttttct tagcaccgat agcagctcca cgagaaactg tcaaaatcgt tccttcaaca
     7261 ggtttcatca ctgccttata ggcaacttcc acacctgatt ggaaggccag agccaagtct
     7321 tgacctgtta actcgtcttt atccttgata gcttgggaaa atccacggaa aagctgagac
     7381 gtaatcactc ctgagttccc acgcgcaccc atcaaaagcc ctttggcaag aatgctcgct
     7441 acttctccaa ctgtagaagc tggcttgtct gcaacttctt tagcaccatt ttcaatggtc
     7501 attcccatat ttgtcccagt atctccatct ggaactggaa agacgtttaa tgaattgaca
     7561 tattcagctt gcttattcaa gcgagttgat gcagcctgca ccatttcttg aaataagcta
     7621 gtagtaattt ttgacacggt tattctccta caactttgat attttgaatg tagacattta
     7681 cagtctgagc agtaattcca agctggtttt ccaagctaaa ggcaacacgc tcttgaatgt
     7741 tttttgacac ttcactaatc tttgttccgt agcttaacac ggtatataca tcaactgcaa
     7801 tactgccatc ttcggctgcc tttacgacga cacctttaga ataattttcc ttacctagca
     7861 gggcttggaa attatctttg agggcatttt tactagccat accgaccaca ccaaaaatct
     7921 cagttgctgc tccacctacg acggttgcaa tcacttcatc tgttagttcg atttgaccat
     7981 cttttgtatt aatttttaca gtcatccttt ttacctcaac tagttgatac tctattttat
     8041 catatttcag cccaagtgta aaagcagaat gatgtatcag cggatattta ctctattttt
     8101 caaacgatgt tatacccaca ataaaagaaa aaagacccta aggtctcctt tgcttttatt
     8161 attaaacgcg ttcaacttta cctgatttca aagcacgagc tgaagcccaa acttttttag
     8221 gtttaccatc gataagaaca gtaacttttt gaaggtttgg ttttacggca cgttttgttt
     8281 ggttcatcgc gtgtgaacgg ttgtttcctg atacagtctt acgacctgta aagtaacata
     8341 ctttagccat tgtgttttcc tcctattaga tctaatatag cggatgtgct agcaccacat
     8401 accgtactat gttatcacac tttcttgttt tttgcaaggg aattggaaga ttttttattt
     8461 gtgtcttaaa tcaggtcttg cgtgacattt tctgctctcc acatgccatc gttgattaac
     8521 agaacaccag aattaaaatt atgtgtataa aaatcatctc taactgcagc taagggtata
     8581 gccgtcaagt ccaaatccca cagctcatct atcgattttc ttacaacaat atctgaatcc
     8641 aaatacagta cacgagactc gcttacatac tttggaataa aatacctaaa aaagccgcat
     8701 atgaaagtcc ctcaaagggg agacgataac ctttcagaat attactgtca atctaaacat
     8761 tcacaatctc actattcaaa gtctctagtc ttttttccat caattggaac cattctcgcg
     8821 gaaggtcatc attaaaaaca taaaacttaa gattataatg atgaacacaa agagatttta
     8881 ttgttgtttc aactttatcc atataagcat tatctgcacc taagacaatc gcttttttct
     8941 cttttttctc ttctttcact ttttatctca tttcttttta ttcccatcat atgtttctac
     9001 gtaaccatta ttttcgccta ttcgttcgta aaaccatacc agtggagatt ttagatgaag
     9061 tcccattacg gtttacaatt tttacattac gacacggagt tttacaaatc gatttcattt
     9121 gccaaacgta gttagtgagg cagttagcta gttcgccaaa tagcgactag cgtccaacaa
     9181 tttggaactt tagttccaat tgttggtact gagtcacatc ttctcctcta actctacgtc
     9241 tggatacttg tcagcaaacc agcggagggc aaagtcattt tcaaagagaa agactggttg
     9301 gtcaaaacgg tctttggcta agatattgcg acttgacgac atccgttcat ccaagtcctc
     9361 aggcttgatc caacgaacgg tctttttacc cattgggttc ataactactt ctgcattgta
     9421 ctcgccttcc atgcggtgtt taaagacttc aaactggagt tgacctacag cgcctagcat
     9481 gtactcacct gtttggtaat tcttataaag ctgaacggct ccttcttgca ccaattgctc
     9541 aatccccttg tggaaggatt tttgcttcat aacattctta gcagaaactt tcatgaaaat
     9601 ttcaggagta aaggttggca gtggttcaaa ttcaaacttg tttttaccaa ctgtcaaggt
     9661 atccccaact tgataagtac cggtatcgta aaccccgata atatcacctg ccacggcatt
     9721 gatcacattc tcacgacttt ccgccataaa ctgggtaaca ttagatagtt tggccccctt
     9781 accagtacga gggagattga cactcatgcc acgctcaaat tcgcctgata cgatacggac
     9841 aaaggcaata cggtcacggt gacgaggatc catgttggct tggattttaa agacaaagcc
     9901 tgagaaatcc ttgtcataag gatccacaat ttcaccgtct gttttcttgt gaccatgtgg
     9961 ttctggagca aacttgagga agatttcaag gaaggtctgc acaccaaagt ttgtcagggc
    10021 tgaaccgaaa aagacaggcg tcaattctcc agccagaata gcttcctctg aaaactcatt
    10081 cccagcttca tttaaaagct caatgtcatc cttgacttgc tcgtagaaag gattgctacc
    10141 aaagagtttg tccccgtctt ctagactggc aaaacgctca tcccctttgt aaagctctaa
    10201 acgttggtta tagaggtcat acaagccctc aaaggctttc cccatcccga taggccagtt
    10261 catagggtag ctagcaatgc ccaagatttc ttccaattct tgcaagagat ccaaaggctc
    10321 acgaccgtca cggtccagct tgttcataaa ggtaaaaact ggaatgccac gatgtttcac
    10381 aacctcaaac aatttcttgg tttgagcctc gatccccttg gcagagtcca cgaccatgac
    10441 cgcagcatcc accgccatca aggtacgata ggtatcttct gagaagtcct cgtgccctgg
    10501 cgtgtctaag atattcacgc gcttgccgtc gtagtcaaat tgcataacag atgaagtaac
    10561 agaaatccca cgttgcttct cgatatccat ccagtcagat ttagcaaaag tccctgtttt
    10621 cttccctttt accgtaccag cctcacgaat ctcaccccca aagtagagta actgctcagt
    10681 gatggttgtt ttccccgcgt ccgggtggga gataatggca aaggtacgac gtttcttaat
    10741 ttcttcttga atattcataa gttctctttc tttgattctc tatttttctt gtttcaatag
    10801 ctgagaatga tttttacatt ggattttacc attcctttca acattccatt atatcggatt
    10861 ttagcatttt tttcaatttc tatttctttt cacttccccc tcccttattt ataggaaaat
    10921 atggtaaaat agaacagact aaaaatcatc atttcacgaa aggatgcaag atgaaaatta
    10981 cgcaagaaga ggtaacacac gttgccaatc tttcaaaatt aagattctct gaagaagaaa
    11041 ctgctgcctt tgcgaccacc ttgtctaaga ttgttgacat ggttgaattg ctgggcgaag
    11101 ttgacacaac tggtgtcgca cctactacga ctatggctga ccgcaagact gtactccgcc
    11161 ctgatgtggc cgaagaagga acagaccgtg atcgcttgtt taaaaacgta cctgaacaag
    11221 acaactacta tatcaaggtg ccagctatcc tagacgatgg aggagatgcc taatgacttt
    11281 taacaataaa actattgaag agttgcacaa tctccttgtc tctaaggaaa tttctgcaac
    11341 agaattgacc caagcaacac ttgaaaatat caagtctcgt gaggaagccc tcaattcatt
    11401 tgtcaccatc gctgaggagc aagctcttgt tcaagctaaa gccattgatg aagctggaat
    11461 tgatgctaac aatgtccttt caggaattcc acttgctgtt aaggataaca tctctacaga
    11521 cggtattctc acaactgctg cctcaaaaat gctctacaac tatgagccaa tctttgatgc
    11581 gacagctgtt gccaatgcaa aaaccaaagg catgattgtc gttggaaaga ccaacatgga
    11641 cgaatttgct atgggtggtt ccggtgaaac ttcacactac ggagcaacta aaaacgcttg
    11701 ggaccacagc aaggttcctg gtgggtcatc aagtggttct gccgcagctg tagcctcagg
    11761 acaagttcgc ttgtcacttg gttctgatac tggtggttcc atccgccaac ctgctgcctt
    11821 caacggaatc gttggtctca aaccaaccta cggaacagtt tcacgtttcg gtctcattgc
    11881 ctttggtagc tcattagacc agattggacc ttttgctcct actgttaagg aaaatgccct
    11941 cttgctcaac gctattgcca gcgaagatac taaagactct acttctgctc ctgtccgcat
    12001 cgccgacttt acttcaaaaa tcggccaaga cattaagggc atgaaaattg ctttgcctaa
    12061 ggaataccta ggcgaaggaa ttgatccaga ggttaaggaa acaatcttaa acgcggccaa
    12121 acactttgaa aaattgggtg ctatcgtcga agaagtcagc cttcctcact ctaaatacgg
    12181 tgttgccgtt tattacatca tcgcttcatc agaagcttca tcaaacttgc aacgttttga
    12241 cggtatccgt tacggctacc gcgcagaaga tgcgactaat cttgatgaaa tctatgtaaa
    12301 cagccgtagc caaggttttg gtgaagaagt gaagcgccgt atcatgctgg gtactttcag
    12361 tctttcatca ggttactacg atgcctacta caagaaggct ggacaggtcc gtacacttat
    12421 cattcaagat ttcgaaaaag tcttcgcgga ttacgatttg attttgggtc caactgctcc
    12481 aagtgttgcc tatgacttgg attctctcaa ccatgaccca gttgccatgt acttagccga
    12541 cctcttgacc atacctgtaa acttggcagg actgcctgga atttcgattc ctgctggatt
    12601 ctctcaaggt ctacctgtcg gactccaatt gattggtccc aagtactctg aggaaaccat
    12661 ttaccaagct gctgctgctt ttgaagcaac aacagactac cacaaacaac aacccgtgat
    12721 ttttggaggt gacaactaat gaactttgaa acagtcatcg gacttgaagt ccacgtagag
    12781 ctcaacacca attcaaaaat cttctcacct acttctgccc actttggaaa tgaccaaaat
    12841 gccaacacta acgtgattga ctggtctttc ccaggagttc taccagttct caataaaggg
    12901 gttgttgatg ccggtatcaa ggctgctctt gccctcaaca tggacctcca caaaaaaatg
    12961 cactttgacc gcaagaacta cttctaccct gataatccca aagcctacca aatttctcag
    13021 tttgatgagc caatcggtta taacggctgg attgaagtcg agctagaaga cggtacgacc
    13081 aagaaaattg gtatcgaacg cgcccactta gaggaagacg ctggtaaaaa cacccacggt
    13141 acagacggct actcttacgt tgaccttaac cgccaagggg tacctttgat tgagattgta
    13201 tctgaagccg atatgcgttc gccagaggag gcctatgctt atctgacagc cctcaaggaa
    13261 gttatccagt acgctggcat ttctgacgtt aagatggaag aaggttctat gcgtgtggat
    13321 gccaacatct cccttcgtcc ttatggtcaa gagaaattcg gtaccaagac tgaattgaag
    13381 aacctcaact ccttctcaaa cgttcgtaaa ggtcttgaat acgaagtcca acgccaggct
    13441 gaaattcttc gctcaggtgg tcaaatccgc caagaaacac gccgttacga tgaagcgaat
    13501 aaagcaacca tcctcatgcg tgtcaaggaa ggggctgctg actaccgcta cttcccagaa
    13561 ccagacctac ccctctttga aatttctgac gagtggattg aggaaatgcg gactgagttg
    13621 ccagagtttc caaaagaacg tcgtgcgcgt tatgtatctg accttggttt atcagactac
    13681 gatgctagtc agttgactgc taataaagtc acttctgact tctttgaaaa agctgttgcc
    13741 ctaggtggtg atgccaaaca agtctctaac tggctccaag gggaagtcgc tcagttcttg
    13801 aatgctgaag gtaaaacact ggaacaaatc gaattgacac cagaaaactt ggttgaaatg
    13861 attgccatca tcgaagacgg tactatttca tctaagattg ccaagaaagt ctttgtccat
    13921 ctagctaaaa atggcggtgg cgcgcgtgaa tacgtggaaa aagcaggtat ggttcaaatt
    13981 tcagatccag ctatcttgat cccaatcatc caccaagtct ttgccgataa cgaagctgct
    14041 gttgccgact tcaagtcagg caaacgtaac gccgacaaag cctttacagg attccttatg
    14101 aaggcaacca aaggccaagc caacccacaa gttgccctta aactacttgc acaggaattg
    14161 gcgaagttga aagaaaacta gacagaacaa aaccagccct aaggttggtt ttttcttctc
    14221 taccaactcc caataactat tttggcttta tttccagagt attttatggt aaaatgaaga
    14281 gtaataatat ttattaaaga ggtaaaaaca tgattgaagc aagtaaatta aaagctggta
    14341 tgacctttga aacagctgac ggcaaattga ttcgcgtttt ggaagctagt caccacaagc
    14401 caggtaaagg aaacacgatc atgcgtatga aattgcgtga tgtccgtact ggttctacat
    14461 ttgacacaag ctaccgtcca gaggaaaaat ttgaacaagc tattatcgag actgtcccag
    14521 ctcaatactt gtacaaaatg gatgacacag catacttcat gaatacagaa acttacgacc
    14581 agtacgaaat ccctgtagtc aatgttgaaa acgaattgct ttacatcctt gaaaactctg
    14641 atgtgaaaat ccaattctac ggaactgaag tgatcggtgt caccgttcct actactgttg
    14701 agttgacagt tgctgaaact caaccatcta tcaaaggtgc tactgttaca ggttctggta
    14761 aaccagcaac gatggaaact ggacttgtcg taaacgttcc agacttcatc gaagcaggac
    14821 aaaaactcgt tatcaacact gcagaaggaa cttacgtttc tcgtgcctaa tctctagaaa
    14881 gaggtcattc tatgggaatt gaagaacaac ttggcgaaat cgttatcgcc ccacgtgtac
    14941 ttgaaaaaat cattgctatc gctactgcaa aggtagaggg tgttcactct ttttcaaaca
    15001 gatcagtgtc tgataccctt tcaaaacttt cactcggccg tggcgtttat cttaaaaacg
    15061 tggacgaaga actcacagca gatatctatc tctaccttga gtacggagta aaagttccta
    15121 aggtagcggt tgctatccag aaagctgtca aagatgccgt ccgtaatatg gctgatgtag
    15181 aactcgctgc tatcaatatt cacgttgcag gtatcgtccc agataaaaca ccaaaaccag
    15241 aattgaaaga tctatttgac gaggacttcc tcaatgacta gtccactatt agaatctaga
    15301 cgccaactcc gtaaatgcgc ttttcaagct ctcatgagcc ttgagttcgg tacgaatgtc
    15361 gaaactgctt gtcgtttcgc ctatactcat gatcgtgaag atacggatgt acaacttcca
    15421 gcctttttga tagacctcgt ttctggtgtt caagctaaaa aggaagaact agataagcaa
    15481 atcactcagc atttaaaagt aggttggacc attgaacgct taacgctcgt ggagagaaac
    15541 ctccttcgct tgggagtttt tgaaatcact tcatttgaca ctcctcagct ggttgctgtt
    15601 aatgaagcta tcgagcttgc aaaggacttc tccgatcaaa aatctgcccg ttttatcaat
    15661 ggactgctca gccagtttgt aacagaagaa caataaaaag aaaaagtgtt tgatagatat
    15721 caaacacttt ttctttgcct cccactatct aaaaaaatga taatagatat aattgtaaac
    15781 aaaaatccag ataggttttg catgattgag aaagttaaaa aaactatggc agagaatcgt
    15841 taatctcaga ttgtcggtag aacgataaac aagggcaaaa aagaaaccaa tcagactata
    15901 atataataaa ctaattggat ctctgtgaga tagtatcaaa tggctaatcc caaagataag
    15961 gcctgataag atgacatcta aatagtactt tgacttagga aagaaggtat tcataaaata
    16021 ccctctatca agagtctcct caaaaacagg accgatgatt acaagcagga caaaagataa
    16081 gatagtcgat aaaaaggttg gttgtccatt tgaaagaacc acggtaaaat actcatcatg
    16141 aatattccta tggttaatca aatgagcata gcgtgcctaa aaattaccga gaatctgata
    16201 aaccacataa gttgcaaata aggagaagac aaatgaccag tcccagctct ttttcttttt
    16261 taacctccaa attaatagaa aaggaaactt cccactaatc ccactgttaa aataagagaa
    16321 tagacatcag ctcctaaccc taaaatgatc gtcacataca atccaattgt ttgtggtaaa
    16381 taggtagata gtaaaataat aagcaaaaat attccaaatt gtcttagttt ctttgtgttt
    16441 ctcatcgtac ttttttgaaa gattaccctg ttcggaagcc gtacttccaa gcatctatat
    16501 aagaattaag tgccccttgc ctcatatagg gagcaaattc tctataatat aaccatctac
    16561 tatatccatc ttcccaaaca gcaagaccac ctgaagtttg ctccaagtcc tcagttgaaa
    16621 gaactgtaaa tgtttctgta cctgtcattg caagtacctt cttaaaatag attgttgtag
    16681 gctcacattt atagtatatt tctttttttg tctattttat agtctatctc ctcaactggc
    16741 aatttttcga cctgaattgc atttttccat aaaaaatgag acctttctag tctcatttag
    16801 tcattcttag tattttctaa atcgttgata gcgttcttcc agcaactctt ctagcggttt
    16861 ttgtgaaagt ctagccagct ccgtttggag ttcttttttg acactcttaa ttagttcttt
    16921 actagaaagt cctacttcag aaatcacctt atccaccacg tccatttcta acagttcatg
    16981 cgaagtgatt ttcatcagtt ctgctgcttc catggcgcga gtaccgtcct tccataaaat
    17041 ggaagcaaag ccttctggac tgagaatggc atagatagaa ttttccagca tccagacacg
    17101 gtccgcgaca gctagagcca gagccccgcc tgaaccacct tcaccgataa taatggcgat
    17161 aataggaact ttcaggtcac tcatttccat gagattgcga gcgatagctt ccccttgacc
    17221 acgttcttcc gctccgacac caggataagc acctgctgta ttgataaagg tcacaactgg
    17281 acggccaaat ttctcagcct gtttcatcaa ccgcagtgcc tttcggtagc cttctggatg
    17341 tggttggcca aaattccgtt tgaggttgtc ttgcaaactc ttgccttttt ggataccaac
    17401 cactgttaca gcttggtctc caagccaacc aataccacca acaactgcac catcatcacg
    17461 aaaagaacgg tcaccatgta attggataaa ttcatcaaaa atgcctgtcg caaagtccaa
    17521 ggttgtcaag cgactctgct cacgcgcttc tctgactatt tttgcaatat tcatctagga
    17581 ctccctccat gcaatctgac taggctagca atcgtatctg gtaagtctct tcttttgaca
    17641 atagcatcca caaagccatg ttctaatagg aattctgcct tttggaaatc ctcaggcaag
    17701 ctttcacgaa ccgtattttc aatcacacga cgcccagcaa aaccaaccaa gctctgtggt
    17761 tcagccagaa tgatatcgcc ttccatagcg aaagaagctg tcacaccacc agtcgttgga
    17821 tctgtcaaaa tggtcaggta aaagagacca gcatttgaat ggcgtttaac cgctgcagag
    17881 atcttagcca tctgcatgag actcatgatt ccttcctgca tacgggctcc accagaggct
    17941 gtgaatagga caactggtaa tttttcgaca gtcgcatact caaacaaacg agtgattttt
    18001 tcacctacaa ccgtacccat agaagccatg ataaaattag aatccataat cccaagagcc
    18061 acagtctgac ctttaataag agcagttcct gtcacaacgg cttcatgcag acctgttttt
    18121 tcacgcatag atgccagttt cttttggtaa ccagggaaat gcaagggatc cttgctttca
    18181 atccctgtaa acaattcttt gaaggttccc atatcaatcg tcaaagccaa gcgttcttgg
    18241 gcagaaatac gaaaggtata gctacagtgc ggacagatac gttcacttcc cagatccttc
    18301 tgatagatgg tatgcttaca gcctggacac tgggaaaata attcatctgg aacctctggc
    18361 ttagcttgag gtttttccct aaccgaacga ttgggattga ttcgaatata cttatctttt
    18421 ttactaaata gagccattga ttcccctttt cggtttaaac tcttaaagtc attttattct
    18481 ttttcttgat atttaggtaa gaaggtttcc atcaagaagg aagtatcata atccccagca
    18541 atgacattgc gatctgaaat gagatcaagc tggaaatctg cattggtctg cactccttca
    18601 atctctaatt catagagggc acgttgcatt ttcatcaagg cgtcaaaacg attttcgccg
    18661 tgtactatga ttttggcaat catactatca taataaggcg gaatggtata acctggataa
    18721 actgctgaat ccacgcgcaa gccaactcca ccacttggca gatagagatt agtaatctta
    18781 cctggacttg gagcaaagtt aaaggctggg ttttctgcat tgatacgaca ctcgatggca
    18841 tgaccgcgta ggacaatatc ttcttgctta acagacaaag gctgacctgc cgcaatgcaa
    18901 atctgttcct taacgatatc aacacctgaa acaaactctg ttactggatg ttctacctga
    18961 acacgagtat tcatctccat gaaatagaaa ttgctacttg cttcatcaag aagaaattca
    19021 atggttcctg cattctcata gccaacaaac tctgccgctc gaacagcagc agcacctatt
    19081 tcatgacgca gcgtttttcc gattgcaatc gagggacttt cttccaaaac cttttggtta
    19141 ttcctttgaa gagaacaatc ccgttcaccc aagtgaatca catgtccatg ctcatcacct
    19201 aggatttgaa cctcaatgtg ccgagctgga tagataaccc gttctatgta catggcacca
    19261 ttgccataat tggccttggc ctcactagag gcagtttcaa aggcagaaac gaggtcatct
    19321 ggtttttcaa ccttacgaat ccctttacca cctccacctg ctgaagcctt gagcataaca
    19381 ggatagccaa ttttttcagc aacaatcaaa gcttcttcag agttatgcac ttctccatct
    19441 gaacctggta taacaggcac acctgcttta atcatctgag cacgcgcatt gatcttatcc
    19501 cccatcatat ccataacatg accagatgga ccgataaact tgatacctac ttcttcacac
    19561 atggtcgcaa atttggaatt ttcactgaga aatccaaaac cagggtgaat agcttctgcc
    19621 tcagtcaaga ctgcagctga tagaactgca ttaatattga gataagactc tgttgccttg
    19681 ccaggaccaa tacaaactgc ttcatctgcc aacagcgtat gaagagcttc cttatcagca
    19741 gttgaataaa ccgctaccgt cgcaatcccc aattcacgtg ccgcacggat aatacgaacc
    19801 gcaatttcac cacgattggc aattaaaatt tttcgaaaca tggagaacct ccttagttcc
    19861 caattgcaaa agtaagggta ccactggctg caagcttgcc atccacttca gcctttgctt
    19921 caaccacagc tatggtgcca cgacgtttta caaaagtcgc tgtcataacc aattggtcgc
    19981 ctggtacaac ttgcttcttg aacttaacct tatccatacc agcgtaaaag accagttttc
    20041 ctttattttc aggttttgat aactccaaca caccagcagt ttgcgccaag gcttccataa
    20101 tcagaacacc tggcataact gggtattgag gaaagtggcc gttaaagaaa ggctcgttga
    20161 tggtcacatt tttgatagca acaatggtat cctcgctcac ctccaagaca cggtccacta
    20221 gaagcatagg ataacggtgg ggaagggctt ctttgattcc ttgaatatcg atcatttgat
    20281 acgtaccaat cctttaccaa actcaaccat ttcttcgtta gagacgagaa tttccgttac
    20341 cacaccatcc ttaggagctg ggatttcatt catgactttc atggcttcga taattaccaa
    20401 tgtttgacct tttttgacac tatcaccaac tgtaacgaag gcaggtttat ctggtccagc
    20461 agccaagtaa accactccaa caagtggact ctctacaaga tttccctcac tagccacact
    20521 tgcttcagct ggagctggaa cttcttctgc tacagtctct gctggagcag atgtaggagc
    20581 tactggactc ggtgttgcta gaacgggtgc tggagcgact tgagttgcaa cttcaggcac
    20641 aggtctcgct tcattcttgc taaactgcaa ctcatccgtc ccatttttat aagaaaattc
    20701 tctcaaactt gactggtcaa attgagtcat caagtcttta atatcgttta aattcatact
    20761 tatctattct cccaacgttt gaaagcaaga actgcattgt ggcctccaaa accaaaagta
    20821 tttgaaatag cgtatggaat ttctttctcc aagccttgtc cataaacgac attagcttcg
    20881 atataatctg atacttcact tgtcccagct gtcattggta caaagttatg acgcatagct
    20941 tcgatggtga cgatagcttc tactgcaccc gcagccccca gcaaatgtcc tgtaaaagac
    21001 ttggttgatg atacaggtac ttccttacca agaacagcta cgatagcacc actttctcct
    21061 ttttcattgg caggagttga cgttccgtga gcattgacat aggctacttg ctctggagaa
    21121 atctcagctt cttccaaggc tagtttgatg gccttgatag ctccctgacc ttctggatgt
    21181 ggagaagtca tgtggtaggc atcacaagta tttccgtaac caaccacttc agccaggata
    21241 gtagctccac gtttttcagc gtgttcaaga ctttctagaa ccaacatccc tgaaccttca
    21301 cccataacaa acccattgcg atccttatca aatgggatcg aagcacgagt tggatcctct
    21361 gtagtagaga gagctgttaa ggcttggaaa ccagcgatgg caaaaggtgt gatagaagct
    21421 tctgttcctc ccaccaacat cacatcttgg aaaccaaact taatggagcg gaaggcatcc
    21481 ccaatcgcat catttgatga agagcaggca gtattgatag atttacaaac accgtttgca
    21541 ccaaaacgca tggctacatt cccagaagcc atatttggta aagcttttgg aagagtcatt
    21601 ggtttgacac gtttgggtcc tttttcatga aggcgaagta cctgatcttc aatttccttg
    21661 attccaccaa taccagatgc aacgataaca ccaaaacgat ccctattaag agcctctaca
    21721 tcaagattgg catgatttac agcctcttgg gctgcataca aggcatataa agaatagtta
    21781 tcaaaacggt tggtatcttt ttttacaaag tatttatcga acggaaaatc ttggatttct
    21841 gccgcattat gcacatcaaa gtcactatga tcaaattttg taatgccacc aatgccgatt
    21901 ttcccagttg ctaaactatt ccaaaattct tctggtgtat ttccgattgg agatgttact
    21961 ccataacctg ttaccactac tcgatttagt ttcattcttt tcacctctag ctttcgctac
    22021 atacttaagc caccatcaat ggcaatcact tgaccagtta gataatcttg gcctgctaaa
    22081 aatactgtca aatctgcaac ctgctctgcc tgcccaaatt ctttcatcgg aatctgagct
    22141 agtgtagctt ccttaatctt atctgataag atagctgtca tatcagactc aatcattcct
    22201 ggagcaatca cattgactcg tatattccga ctagcgacct cgcgtgccac agacttggta
    22261 aagccaatca agccagcctt agaagcagca tagttagctt gaccaatatt ccccatcaaa
    22321 ccaacaacac tagacatatt aatgatagca ccttctctgg ctttcatcat cggtttcaag
    22381 actgattgtg tcatattaaa ggcaccagtc agattgacct tgagcacttt ttcaaaatct
    22441 gcttctgtca tcttgagcat aagagtatct tgggtaatcc ctgcattgtt gaccaaaaca
    22501 tctactgaac ccagttctgc aatagcttga tcaatcatac gcttagcgtc tgcaaaatct
    22561 gatacatctc ctgaaatggg aaccaccttg ataccatagt ttgaaaactc agcgagcaat
    22621 tcttctgaga ttgccccacg actgtttaag acaatgttgg ctcctgcttg agcaaacttg
    22681 tgggcgatgg caagaccaat tccacgactc gaacctgtaa taaagatatt tttatgttct
    22741 agtttcattt ttttcctttc aaaacttcta cttattttag tctatttttc taaaagtgct
    22801 actaaactcg cttgatcttc cacatgagct aagtgagcag tttgatcaat ttttttaaca
    22861 aaacctgaca agactttccc cggtccaatc tcgataaagt tgcttatgcc tgcttcttgc
    22921 atgaccccaa tactttcata gaaacgaacg ggttccttga cctgacgcgt caagagctga
    22981 gcaatgtcct ctttttgcat cacagcagct tctgtattgc cgactagggg acaagtaaaa
    23041 tctgaaaaac ttacctgagc tagagtttca gctagtttct ggctagcagg ttcaaggaga
    23101 gcggtgtgaa agggacctga caccttaaga ggaatcaagc gtttggcacc tgcttcttgc
    23161 aaaagttcaa ccgctcgatc aactgcaacc acttctccag caatgacgat ttgtgcaggt
    23221 gtgttatagt tggctggagt aaccactcca agttcagaag ctttttgaca ggcttcttca
    23281 atgacctcta ctggcgtatt gagaactgct accatcttgc cagagtcagc aggagccgct
    23341 tcttccatat aggctccacg cttagctacc aaggcaaccg catcttcaaa atccaaggcg
    23401 ccgcttgcca ccaaggcaga gtattctcca agagacaaac cagcaaccat atcaggctga
    23461 tagccctttt cttgcaataa acggtagata gcaaccgaag tcgctagaat ggctggttgc
    23521 gtatagcggg tctgattgag tttgtcttct tccgtatcga tgagataacg taaatcataa
    23581 ccgagcacct gactcgctcg atcaatcgtt tctttaacaa tcggatactg atcatagaaa
    23641 tcccgtccca tccctagata ctgggcacct tgaccagcaa ataaaaaggc tgttttagtc
    23701 atttcttaca actcctgccc agcgagaggc ttcttcttga attttcttag cggctccgta
    23761 atacaaatct tttaggattt cttcagctgt ttcttcttta gaaacaagcc ctgcgatttg
    23821 acctgccatg acagagccac cctccacatc accgtgaaca actgctttgg ctagggcacc
    23881 tgctcccatt tgttcaaaga tttctaaatc aggatcttcc tgcttaaagg catctttttc
    23941 agccagttca aaatctctag tcaactgatt tttaatagca cgaacagcat gaccaaagtg
    24001 ctgagctgaa atcgtagtat caatatccct tgcttttaaa attttctctt tgtagtttgg
    24061 atgggcattc gactcttttg caactacaaa ccgtgtcccc acctgtacag cctctgcacc
    24121 tagcataaag ccagccgcag caccttcacc atccgcaatt cctcctgcag caataacagg
    24181 aatagatata gctgtggcta cctgtcgcac caaggtcatg gttgttaatt taccgatatg
    24241 ccccccagct tccattcctt ctgcaataac agcgtctgca ccgatttttt ccatgcgttt
    24301 agctaaagcg acactaggaa caacaggaat aacgattatc ccagcttcat ggaaacgttc
    24361 catatacttg cttggatttc ctgctcctgt tgtgacaact ttaacacctt cttcaataac
    24421 gagatccacg atatcttcca caaagggaga taagagcatg atgttgaccc caaagggttt
    24481 atcagtcaat gatttgattt tatcaatatt ggccttgaca acttctttcg gggcatttcc
    24541 cccaccgata attcctaatc ctccagcctt ggaaacagcc cctgccaaat caccatcagc
    24601 aacccaggcc atccctcctt ggaaaatagg atagtcaatc ttcaataatt ctgtaatacg
    24661 cgttttcata gtgcctccaa ccttccttgc ttacgtaata gttcgatttc accataattt
    24721 gacagtcaaa ctattaccta aacaagaggg agtgggtttc tccctactcc ttctactaaa
    24781 tattctgctt attttgcttg ctcttcaacg taagcaacca agtcaccaac tgttttcaag
    24841 tcattttctg cttcgatttg gatatcaaaa gcatcttcga tttctgagat tacttggaac
    24901 aagtccaatg aatctgcgtc caaatcatca aaagttgatt caagtgttac ttctgatgcg
    24961 tcttttccaa gttcttcaac gataatttct tgtacttttt caaatactgc catgatagga
    25021 ctcctttaaa ataaatagtt tttttataac aatgtgttca ccacatgatt acctaaattg
    25081 taagaatgag cgtgccccag gtcaagcctc caccgaagcc tgatagaaga acagtttggc
    25141 taccatctaa agggatgaga ccttgttcta cacactctga aagtaaaatc gggatactgg
    25201 ctgcactggt attgccatat tccatcatat tagctggaag tttggctcgg tcaacaccaa
    25261 tttttctagc catcttatcc aaaatacggt tattggcttg atgaagtagc agataatcca
    25321 agtctgtcac ctctatagga gattcatcaa tagtctgctt gatagacttg gctacatctc
    25381 gaatggcaaa atcaaagact gtgcgtccat ccatcttcaa aaatgaatct gcactttctt
    25441 gatctgaaaa tggagaatgc aaacctgaat gcccataagt taaacactcg ctgcgacttc
    25501 catcgctatt gagactctca gctaagaaat gctcttgctc gctagcttct aacaagacac
    25561 caccagcacc atctccaaac aacacagctg ttgatcgatc cgaccaatcg actgccttag
    25621 agagggtttc actaccaatc accaagcctt tttgaaagcg accagaagcg ataaactttt
    25681 cagcagttga aagagcaaat acaaatccac tgcaagccgc ggttaagtca aaagcaaagg
    25741 ctttattagc accaatatta gcttgaacac gagcagctgt agagggcatc atcgaatctg
    25801 gagtaatggt agctaggatg ataaaatcca gttcttctcc tgttattcca gcttttgcca
    25861 tcagtttctt agcaacctct gtagccaaat cactggtaga ttctgttctt gaaatatgcc
    25921 tttgtcgtat tcccgttcga cttgaaatcc actcatcatt ggtatccata atctgagcca
    25981 agtcgtgatt tgtaaccact tgctctggca cataatgagc aacctgactt atttttgcaa
    26041 aagccattat ttcaaatcct ccaaaaattg gtaaagatta gtcaaacctt tacccatgac
    26101 agcaatttct tcctcgctca tgccatcaat aattttttct accatggcct tgtggaagcg
    26161 tttatgcagt ctatgaatca agcgaccctt ctttgtcaaa tgcagatgca ccacacgacg
    26221 atcctgttct gaccgaactc gctcaatgta gcccttacgc tctaaattgt tcaaacttgt
    26281 cgtaacagtt ccaagagtta ccatcaactc ttttgacact tgacttggag tcacatctgg
    26341 agccttaccg atgacatcaa tcgtatgcat ttctttgatg gagatatcct taaaacgact
    26401 acctctcaag ttcacttcct caattacaag gacattgtta aatatagatg ttaaatattc
    26461 attgattcgt tggtagtcca tttttcatat ccctccttct tcaaaaaact tttacaatca
    26521 aaacatttga cagaaagaat tatatcaaaa ttcaaagaat gatgcaagta ttttttattt
    26581 tcctgtaaat ttaggtcttc ttctctccga atgagctcgc actccttctt tgaaatcctc
    26641 tgtttgagct aaggatttct gtaggttcag ttctaaagta gcgtaaccct gccagtcttt
    26701 aaattggctc tcccaaacca acttcttaat ggcagcatag gaattacttg aagcacgtct
    26761 taatttttta agaagctgtt ctctcgtctt ttcaagtttt tcagcttcag aaacgcggta
    26821 aaccaacccc cattctaggg ctttttctgc cgttaaagcc tctcctgtca tagctaattg
    26881 agcagcacgc gtcacaccga tactgcggct caagagatga attccccctg catctggagc
    26941 caaaccaaca ccaacaaagg cttggataaa cttagcctta tccgtcgcca aacagaaatc
    27001 tgccgcaaca gccatattcg ctgcggcacc tgcaacagca ccgtcaactt ccattaaaac
    27061 aggtttggct atttgcttga ttttataaga aatcgtattg accaactctg cgatttttgt
    27121 caatgatgga atatcatcct catccactgc ccgcttcatc tctaccaaat ctcccccaac
    27181 tgagaagacc tttccattgg cattgattaa gataaaatgc acagctggat tttcttctgc
    27241 caaagtcaga gcttctaaaa tctcctcaca catgggaata tgaaaaccat ttgcgacctc
    27301 aggacggttc aaggtaagga ttgccaagtc ctcttcaagc tgataaataa tgtgttccat
    27361 cagggctcct ttttatttta taagcagttt atactcttcg aaaatctctt taaaccgcgt
    27421 cagcttccat ctgcaacctc aaaacagtat tttgagctga cttcgtctag cttcttagtt
    27481 tgctctttga ttttcattga gtgttaaatt atttcactgt caaagtatat cttttttagg
    27541 agcagaaagc aagaaaaaat cgactgaaag tgtctcagcc gataagaaag gctcgcttta
    27601 ggctagaaag catcttattt tcttcattga aaaaagcctt tctttctgct ataatcgtat
    27661 aaaatactta ctttaggagt tcttatgaaa gttgttaaat ttggaggtag ttctcttgcc
    27721 tctgctagtc aattagaaaa agttttaaac atcgtcaaaa gcgattcaga gcgtcgtttt
    27781 gtagtcgttt ctgcgcctgg taaacgcaat gctgaagata ctaaggttac ggatgccctg
    27841 attaaatact accgcgacta tgttgcgggt aacgatatta gcaagagcca aagctggatt
    27901 atcgaccgct atgctgctat ggttagtgaa ttgggactaa aaccagctgt gctagaaaaa
    27961 atttctaaaa gcattcacgc cttggccact cttcctattg aagaaaatga atttctctac
    28021 gatactttcc tagcagccgg tgaaaataac aatgccaaat tgattgctgc ctactttaac
    28081 caaaatggta tcgatgcacg ctatatgcac cctagagaag ctgggattgt ggtcacaagt
    28141 gaacctggtc acgctcgcat cattccatca agttatgaca agattgaaga attgacaaac
    28201 accaatgaag tccttgtcat tcctggtttc tttggtgtca ctaaggaaaa tcaaatctgt
    28261 actttctcac gtggaggttc tgatattaca ggttctatca ttgctgctgg tgtcaaagct
    28321 gacctctatg aaaactttac ggacgttgat ggtatctttg cagcccaccc tggtattatc
    28381 caccaaccac actcgattcc tgagttgacc taccgtgaaa tgcgcgagtt ggcctatgca
    28441 ggcttctcag tccttcatga cgaggctctt cttcctgcct accgtggaaa aattcctctg
    28501 gttatcaaga ataccaacaa ccctgaccat ccaggtactc gtatcgttct aaaacacagt
    28561 aatgatgaat ttccagttgt gggaattgct ggtgactcag gctttgtcag cattaacatg
    28621 tcgaaatacc tcatgaaccg tgaggttgga tttggccgca aggttctgca aatcctggaa
    28681 gaacttaaca tcggttggga acatatgcca acaggtatcg acgatctttc tatcattctc
    28741 cgttctcgcc aactaactcc catcaaggaa gaagaaatcc tgcgtcagtt ggttcaaaag
    28801 gctaaagtag atcatgcaga aatcgaacac gacctttcta tcattatgat tgttggtgaa
    28861 aaaatgaaga gccatatcgg agtgactgct actgcgacac gcgctctatc tgaaaacaaa
    28921 atcaacatcc agatgatgtc tcaaggttct agtgaagttt ctattatgtt tgttgtcaat
    28981 aaagaccaag agaaagcagc aattaaagcc ctctacaatg ccttttttgg agaaagtaag
    29041 gaagactaag catggcccaa tcacttaaca aaacagtact cctcagtacg acaggcactt
    29101 cctacctctc tatcgctggg aaagttggga aattccttgt cggagatcag gctttggaat
    29161 tttacccaga tgtcaatgtc gaacaattta tccagattcc ttggagccat atcaatcaga
    29221 ttggagccaa tgtcactgga cgcaaaatca gccgccactt cgaagtcttt acagacaaag
    29281 gaaaattcct ctttgcttca aaagactcag gtgccattct caaaattgca cgcgaaaaac
    29341 taggcaatga caaggtcgtc aaacttccga ccctgattca gacaattagc caaaaattta
    29401 aaaatctatt tgcaaaaaag tagaaacttt agtataatca tagcaacgga taagtaggtt
    29461 atcttcttct ttcagaaagt ctgcgggtac tgcgagcaga taggggggat aggtgaaatt
    29521 ctaccgttag taacaattac atacacaatc aagtgcacac ctgtgaagtt ggatggaacc
    29581 gtggccctgc cactccaacg ttttgtcagg cgtgcttttt tcataaagga gttcttatgt
    29641 tagatatcaa acgtattcgt acagattttg aagctgtcgc agaaaaatta gctacacgtg
    29701 gtgtagatgc tgctgtcttg aatgaaatga aagaaatcga tgctaaacgt cgtaacatct
    29761 tggtcaaggt tgaaactctc aaagcagaac gtaacacagt ttctgctgag attgcccaag
    29821 ctaagcgcaa caaggaaaat acagatgaca agattgctgc catgcaaaat ctatctgctg
    29881 aggttaaagc cttggatgct gaattggcag aaatcgatgc taaattgaca gaatttacaa
    29941 cgactcttcc aaatatccca gctgacagcg ttcctgttgg gactgacgaa gacgacaatg
    30001 tggaagttcg ccgttggggt actccacgcg agtttgactt cgaacctaaa gctcactggg
    30061 atctcggtga agaccttggt atccttgact gggaacgcgg tggtaaggta acaggcgctc
    30121 gcttcctctt ctataaaggc ctcggtgctc gtttggaacg tgctatctac aactttatgt
    30181 tggatgaaca tggaaaagaa ggctatactg aagtcatcac accttacata gtcaaccatg
    30241 attctatgtt tggtactggt cagtatccaa aatttaagga agatactttt gaactcagcg
    30301 ataccaactt tgtcttgatt ccaactgctg aagttcctct gacaaactac taccgtgatg
    30361 aaatcttaga cggcaaagat cttccaatct acttcactgc catgagtccg tcattccgtt
    30421 ctgaggctgg ttctgccggt cgtgatacgc gtggcttgat ccgtttgcac caattccaca
    30481 aggttgaaat ggtcaaattt gccaaaccag aagaatctta cgaagaattg gaaaaaatga
    30541 cagccaacgc tgaaaacatt cttcaaaaac tcaaccttcc ataccgtgtc gttgctctct
    30601 ctactggaga tatgggcttc tcagctgcga agacttacga cttggaagtg tggattccag
    30661 cacaaaacaa ttaccgtgaa atctcaagct gttcaaacac agaagatttc caagcccgtc
    30721 gtgcccaaat ccgttaccgt gatgaagcag atggcaaggt gaaactcctt cataccttga
    30781 acggctctgg acttgcagtt ggacgtacag tggctgcaat tcttgaaaat taccaaaatg
    30841 aagatggttc tgtgaccatc ccagaagcac ttcgtccata catgggtgga gctgaagtca
    30901 tcaaaccata aaaaataagg tttagctatt tctagctaga ccttttttcg taaccaaatc
    30961 agataagcac ctagtacaaa gaataaaata gttaggcata taatggtttc agccaatacc
    31021 aggtaatcca gaaatggaag tttcaaaatt ccctgagcca tcttgagcga ggtcgctgtg
    31081 ataatggttg ggaaggtgag ggctgagaag gctggttgaa aaccttgttt taaaatgttg
    31141 ggcagacgag ttaaaacaaa gaaaaagaag gattgagaag ccaaaatcat gacaatcaag
    31201 acccaagtcg gcaggctggt tcctcctact cgaactagag aagccaagag tagagagaaa
    31261 ggagcacagt agattccttc ttgtccaagc aaggctagtg ggagtggatg tttctttaaa
    31321 tcgctataaa taaggggata gagatagaag gtcaagagaa aaccaaaact caaggtcgca
    31381 taggcaattt cgataatacc taccagagga taggtcaagg cagccactgc tatccccaca
    31441 tagagaaccg tccaacttgg agtggcatga accctccgcc ctgaacaagc aaacttgatg
    31501 gtaaaaccag caatcaaggt caaatccaag agaaatgaaa accaccaaat cccttgtgct
    31561 accaaaggaa gataagagaa tacgcgaaag acataggtcg ataaaatcat cccagccata
    31621 ggaaaggttg ccattcctga caaaagaggg ggcttggtca attcttgctt ggtttctttc
    31681 caattaaaga gatgcagaat tagaaagtaa atccataaaa ccaaaccagt cagactaaaa
    31741 agatgggata gaaccggcaa cgtatctaaa ataagatttc cagctcctgc caaacctagc
    31801 aaacaacctg aaaatactaa ggggagtttt ttcatcctaa cctccaataa tcatgttagt
    31861 ttcagtataa cataaaagcg cttaaatgag gatttaaaaa aacgaagtcc gcttatttca
    31921 gacttcattt tactcagata tgaattaggc ataaggttgc aattctggat taattggtgt
    31981 attagctaag ttgttggcat agttacagag gattgctagg ctgacaccaa aaaccacatc
    32041 caaggcattt tgttgagtgt agccagcttc taaaaactca gacaaggctt catctcctac
    32101 acgacccttg gtattgataa ctgccaaggt aaacttagct agggtatcca atttaggatc
    32161 tgtttcaatt ggagtacgat tgcgaagagc ttgaatcaag tcatcattca tctggatttg
    32221 tttgatggaa aaggctgtgt gacctgcgac acagaaggca caaccattgg tcacggctgc
    32281 cgtgatttgc accacttcac gctcaacggg tgtcaggctg ttgcgacggt ggatagatga
    32341 gacaatttgg taggcttcta aaacagtcgg ggcattggcc aagagaccga ttaggttggg
    32401 aatatagcca ttgttgtctt tttctactgt ttcaagaatt tctttcactt ctgctggtgc
    32461 tgactctact gtatggatag taaatgttgt cataagatac ctcttttctt attattgaca
    32521 ctaatattat tggaaaatct tataaaatcc tgattcctaa gtttatctaa gataaagctt
    32581 tattctctca taagattttc gttgttatat tagtttatca cacttccaat cacttgcata
    32641 atatatatta tatatcaggc tgataaaaat tatttatagg caaaaaaatc acacgagctg
    32701 tgtgattcca ttatttgtca aaatactttt tagtttcagc aataacgact ggcgacaaga
    32761 ccaagagggc aatcaagttt ggcagagcca tcaaggcgtt aacgatatct gcgataatcc
    32821 agaccatatc caactcgata aatcctccta acaagaccat gagcacaaaa aacacacggt
    32881 agagccagat aaagcgagcc ccaaagagga actcaaaaca gcgttctccg taatagttcc
    32941 aacctagaat cgttgtaaag gcaaaaagta caaggaagat ggtcaagagg gcaggcccaa
    33001 agtgtgaaaa gactgttgag aaagctgact gagtcaaggc aaccccattc aagtcaccac
    33061 tccaaactcc agttaccaag atggtcaaac cagttagagt acaaatgatg agggtatcaa
    33121 taaaggttcc tgtcatggaa atcaaacctt gctctactgg ttcatttgtc ttggcagctg
    33181 cagctgcaat aggagcagaa cccagaccag attcgtttga gaacacacca cgcgccacac
    33241 cattttgaat agccatccga acgctagcac cagcaaatcc acctaccgca gcaaggggac
    33301 taaaagctga ggtaaagact aaagcgattg tgccagggat ttttccgata ttaaagaaaa
    33361 taactgtaag agttcctaag atataaatga gggccataaa aggaacaaca gtagttgaaa
    33421 ccttagaaat agacttgagt ccaccaaaga ctgcaatcgc tacaaagaca gacaagacga
    33481 gagctgtgat ggctggcgaa atcgtcgttg tattttggat agattctgta atcgagttga
    33541 cttgggtgaa ggttccgatt cccaagagag caaccaatac tcctgctact gcaaacaaaa
    33601 cagcaagtgg tcgccacttt tctcccatcc ctagaaggat atagtgcatg ggacctcccg
    33661 ctactgcacc atggtcgtcc ttggtgcggt atttgatggc caagagtcct tccgcatact
    33721 tggtagccat tccaaagaaa gccgccatcc acatccaaaa tagagctcct ggtccaccaa
    33781 ccttgatagc cgtcgcaact cctatgatat ttcctgttcc aacagttgat gccaaggctg
    33841 tacacagagc tgcaaaactg gatacatcac catgtccctt atcctggata aaaataagct
    33901 gaaaggcctt gggcagacgc aaaacctgca agagtcctag ccgcatggtt aggtaaatcc
    33961 ctgttccgac caataaaatc aagaggggcg gtccccaagc aaaagcatcg attgatttaa
    34021 gcaattctaa catttccttc tcctatcgtt tcaaccccaa aagaaagagc acatgcaaga
    34081 tacatgtact ctggaatgct tagataaatg ctaaaaagcg gtctatccta gctctgtcct
    34141 tttacctgag agtttgagca gttgcctgcc ttgccccttc ggtgccttta cggtctctcc
    34201 agagttccgt ccatttacag tcatggaaaa tcaaacgatt ccccacttct attaaacttc
    34261 attcggtgtt ggtatttaat tgattctaat tttacaaaaa atgttggctt ttgtcaatgt
    34321 gtttattagt aaaaattagt tcaacagttt ttactttata aagtccagaa tactgctatc
    34381 ctttaaaagt gacaatagtc gcaccactgc ctccagcatt ttgtggggca tagccgaaac
    34441 tcttgacatg tttgtttctt tgcaagtatt tggtaactcc ttcacggatg actcctgttc
    34501 cgataccatg gatgatatca acttgagcca tattgttaag caaggcttgg tcgatgaagg
    34561 tatctagctc attcatggct tcttcatagc gcttgcctcg aagatccagt ctagcttgag
    34621 gtcctcgccc agaagttcgt ttcacaacat tgacctgttt cttcttgact ggtttttctt
    34681 gctgggcttg aacaagatca aactctttct cttccaaggt catcttaatc aagccaactt
    34741 gggcttccca gcgaccgtcc ttgagttgac tggtcaaggt accacgctga ccataactga
    34801 gaaccacgat atcatctccc acctttggag ctcgtttttt cttggccttt tgaaggacct
    34861 tatttttaga caagtccact ttttcaggag ccaatttttt caacttggcc ttggcttcaa
    34921 tgatttcgtg gggcttgagt tgggatttac tgtggagatt tttgagaatc tggtcacttt
    34981 cacttagggc catatccaca atctcagcag cctgttcacg cgccttgtta agctcggttt
    35041 ccttttcacg attaagctcg ttgtagagtt tttttagcgc acggttcatc ttgagatttt
    35101 cttgctccac ctcacggata ttgtccaaac gtttgcggct ttccagcgtc tgctcttcta
    35161 attgctcaat gatacgattg acgtcattgt cctgatcgat ctgctgactg gcatctccta
    35221 cgataacttc agataggcct agacgtttgg caatttcaaa ggcattactt cggccaggaa
    35281 caccctgcat aaagcgatag gtcgggcgaa gagttgcagt atcaaactcc atactggcat
    35341 tttgcacaaa ggctgtctca ataccgtagg ccttgagttc tggatagtgg gtcgtcgcca
    35401 tggtcttgat ttgacgcagg cgaaggtcct ccagaatagc catggcaagg gctgctccct
    35461 cttggggatc agtaccagcc cccaactcat ccaaaagtaa gagtgaatgt tggttgacct
    35521 tgccaagaat atccacgata ttggtcatat ggctagagaa ggtagacaag ctctgctcaa
    35581 tagactgctc atctccaata tcagcaaaga tttcttcaaa aataccaaca cgacttccct
    35641 tgtctgctaa aatcggcaat cctgactggg ccatgacctg tgtcaagccc agagttttga
    35701 gcatgatggt cttcccacct gtattgggac ctgtaatgac aatagctgtt aaatcttgac
    35761 caaaatggac atcatttgcg acggcatttt tgaccaaagg atggcagaca tggagcagtt
    35821 gaatctcttg attttctgac agctgaggca cgactgcttg tctttcttgg ataaatcgaa
    35881 ccttggcacg aatcaagtcc agatgaccga taatccaagc gtcattagca atctcagccg
    35941 catgagggcg gacacgctca gaaatttctt ggagaatgcg aagcatttca tagcgctcat
    36001 ctgctcgcag actagcaatt tcttcgctca gtttgactac ctcacgtggt tcgatataga
    36061 cggtgtttcc actagcagaa atatcatgaa cgacacctgc aatcttattg cggtaggtgt
    36121 ttttgactgg taaaacctga cggccatttc tgctagcaac aattccttcc gtcaacatct
    36181 gcgctttttg cttgagcaag tcttgtaaaa catcgcgtac ctgactctcg ctatcatgta
    36241 tttttcgacg gattcgcgcc aattcttcac tggcaaaatt ttcaatgaaa cccgcatcat
    36301 taaaggcctg aagatttcct tgtaattgcg gaaaatcatg taatttctca aaccaaaggg
    36361 ctaattcttc caagctgaca ttttccagat tggcataaaa actttgaagt tctcggctgg
    36421 caagaagcac gcgtttcaag agtaggaact cctcgatatt gagatccgct cccatctcca
    36481 acctcttgca gactcctgca atttccttag ttgagagaat agtaaaatgc ggttgctcga
    36541 cgaaaagagc ctgcatttct ttcatctcag caaaagcctg tttgatttta tctgctttgg
    36601 cagtcggagc cagctgtctc aattgctcca agccctgctc ggtcaacaaa tgaggctcaa
    36661 acaaggcctt gaccttatcg aactctaatg tttctaatat tttcttattc atctttattt
    36721 ccttgtcttt gaatgtctag gtgtggtagc tttttacatt ctgatagatt cgagtcaaat
    36781 ctgtaaacaa agggctagga aaactcctgc cctttttatc cgattaaatt tgtcacccag
    36841 atttgtttga gccaactggt tgttatcggt atgctctgga tgatgtgttt tgcgacgata
    36901 ctcttttcaa gaggattttg tataactgcc atggggatgg tcgccaagat tgtcaaggcc
    36961 atttgcaaga caaataaggt caccaacatg gacaagatac ctgctgaaac ttggaacaac
    37021 ttaccaccca gttttttgct aggaatcaag tgtaagagaa gaccaagcaa acgaccaatg
    37081 ctatagaaaa tcccaaatac aagcaagtag ccgatacctg cataaaagac cttatccagc
    37141 tgaaagagtt gatccgatgg gaaaaagaaa gtcccctgac cttcctgcga atttgcataa
    37201 gggagcaata aatggaattg ctctccaagc cccttataaa actggccagc cataaaagcc
    37261 gatgccatgg ctgaaatcag gtaataaacc tgtaagagca ggcctctccg atagccgata
    37321 taaaatcccc aaaccaagac caatagaaga aggaatgaaa tcataaggaa tcctcaatct
    37381 tgctctgttc ttgcttacaa gtcacaagct tgtgacggag ttcttctagc tcttgctcct
    37441 tatcgtcaaa ttcaatctca cggctgagct gagttgataa acagttgact gccaacaaaa
    37501 gagcgattgt ttcatcatct gcgctaggca tttgttcttt aattgcttgg tatttttctg
    37561 tcgcaacctt agcgatttcc tccataaaaa ggttatcatg ttcgcttgtc aaggttaacg
    37621 attttttccc gaatgtaaat ttgaatcgat ttagatttgc cataaaattc acctcacgat
    37681 attataccaa aattcgctaa ttttgtcagt ttttacaaat tttactgctt ttgtggtaca
    37741 atagaaacta tggcaagtat cacactcaca ccaagcgaaa aggatattca ggcttttctt
    37801 gaacactatc aaaccagtct ggctcccagt aagaatccct atatccgcta ctttttgaaa
    37861 ctacctcaag caacggtttc tatctatact tctggaaaaa tcttgcttca gggtgaaggg
    37921 gctgagaaat acgctagttt ctttggctat caacttgtag aggaaaacag ggggcaaaat
    37981 cttcctttaa ttgggacaga tgaggtggga aatggttcct actttggtgg gcttgcagtt
    38041 gtggctgcct ttgtcacatc tgaccagcac gactttttac gaaaactcgg tgtgggggat
    38101 tctaagactc tgaccgacca aaagatccgt cagattgctc ctattctcaa ggaaaaaatc
    38161 cagcaccagg cactccttct ctcacccagc aagtacaacg aggtcatcgg agaccgctac
    38221 aatgctgttt cggttaaggt tgccctccat aatcaggcta tctatctcct ccttcaaaaa
    38281 ggtgttcagc ctgagaaaat tgtgattgat gcctttacca gtgctaaaaa ttatgacaag
    38341 tacttggcac aagaggccaa tcgtttcagc aatcctatca gcttagaaga aaaggctgag
    38401 ggcaaatact tgtctgtcgc agtttcttct gtcattgcgc gtgatctctt tctggaaaat
    38461 cttgaaaatt tgggacgaga actgggttat cagctcccaa gtggagctgg aacggcttct
    38521 gacaaggtgg ctagccagat tttgcaagcc tatggtatgc agggactcag cttctgcgcc
    38581 aaattgcact ttaaaaacac tgaaaaagcg aaaaaacgct tagaaaggta agttatgaat
    38641 tcatttaaaa atttcttaaa agagtgggga ttgttcctcc taattctgtc attactagct
    38701 ttaagtcgta tctttttttg gagcaatgtt cgcgtagaag gacattccat ggatccgacc
    38761 ctagcggatg gcgaaattct cttcgttgta aaacaccttc ctattgaccg ttttgatatc
    38821 gtggtggccc atgaggaaga tgggaataag gacatcgtca agcgcgtgat tggaatgcct
    38881 ggcgacacca ttcgttacga aaatgataaa ctctacatca atgacaaaga aacggacgag
    38941 ccttatctag cagactatat caaacgcttc aaggatgaca aactccaaag cacttactca
    39001 ggcaagggct ttgaaggaaa taaaggaact ttctttagaa gtatcgctca aaaagctcaa
    39061 gccttcacag ttgatgtcaa ctacaacacc aactttagct ttactgttcc agaaggagaa
    39121 taccttctcc tcggagatga ccgcttggtt tcgagcgaca gccgccacgt aggtaccttc
    39181 aaagcaaaag atatcacagg ggaagctaaa ttccgcttat ggccaatcac ccgtatcgga
    39241 acattttaag aaacctaaga ggccgagaat caccaatctc agcctcttct tctatcgtga
    39301 gaaaatgatt ggtaactatc taaacttacc agaacagaaa cacctcaact ctcacctatt
    39361 catgcaaagg aattttatgg aagtttattt ttcaggaacc attgaacgga ttatttttga
    39421 aaatcccagc aatttttatc gcatcctcct cctagaaatc gacgatacgg acgcagagga
    39481 ttttgatgat tttgaaatca ttgtcacagg aaccatggct gatgtaattg agggcgaaga
    39541 ctatactttt tgggggcaaa ttgtccagca ctccaagtat ggagaacaac tgcaaatcag
    39601 tcgttatgat cgcgcaaaac caactagtaa gggcttggtc aagtactttt caagtagcca
    39661 tttcaagggg attggtctca agacagctca gaaaatcgtg gatacctatg gcgaaaatac
    39721 cattgacgaa attttgcaac acccagaaaa gttagaaggc atcgcaggac tctctgccaa
    39781 aaatcgcgag gctttcgtct ccactctccg tctcaactac ggaacggaga tggttttggc
    39841 caaactagcc aactacggca ttcccaacaa actagccttt cagattcaag acttttacaa
    39901 ggaagaaacc cttgatgtgg ttgaaaatta tccctaccag ctggttgagg atatcaaggg
    39961 tttgggcttt accattgctg accaactagc tgaggaacta ggcatcgaaa gtcaggctcc
    40021 tgaacgcttc cgcgctggtc tagttcacag tctttttcag gcctgtatgg aaacagggga
    40081 cacctatgtt gaagcacggg atttgctgga acaaaccctt actctccttg agtcttcccg
    40141 tcccgtggaa ctagacccca gccaagttgc ccaagagctc tcctacctga tcgaagaaga
    40201 caaagttcag cagattgata ccaagatctt tgacaacagc ctctttttcg ctgaggaagg
    40261 catccgcagt cacttggttc gtatccttga aaaaggaaaa cagaagagtc atgatttaaa
    40321 aaccattcaa aaacatatca ctactatcga gaaagagctt ggaattgagt atgataacat
    40381 tcaaaaacag gctatctgtg atgctatcca gaataaggtc tttatcctga caggtggacc
    40441 tggtactggt aagacaactg ttatcaatgg gatcatcgct gtttacgccc tgttagaggg
    40501 acttgacctc aggaagaaaa gcaacctgcc cattcttctt gctgctccaa ctggtcgagc
    40561 tgctcgtcgc atgaatgaat tgacaggttt gcctagcgcg accatacacc gccacttggg
    40621 aatgacaggt gacgatgata ccagtcatct ggaagattac ctagatgctg actttatcat
    40681 cgtggatgaa ttctctatgg tggatacttg gctggccaat caactcttct ccagtatctc
    40741 ttctaacagt aagatcctca tcgtaggtga cagtgatcag ctaccttctg tcagtcctgg
    40801 acaggttcta gcggatctgc ttcatattcc tttgattcct cagactcgct tggaaaaaat
    40861 ttatcgacaa agcgaagaat caaccatcgt caccctagct agtcagattc gacagggcat
    40921 cttgccagct gatttcaccc aaaaaaaagc tgaccgttcc tactttgaaa ttgctagtgg
    40981 ccatattcct gccaccattg aaaaaatctt aggtgccgcc ctcagaaatg gtattcctgc
    41041 ccgtgatatc caagttctgg ctcctatgta ccgagggacg gcagggattg atgctatcaa
    41101 tcagctcatg caagacctgc tcaatccccc acaaaaagat caactcagtt ttgaagctcc
    41161 tcagtgccac tatcgtaagg gagacaaggt cattcatttg gttaacgatg ctgaaatcaa
    41221 tgtctttaat ggggatttag gagctatcac agacctgatt cctggtaaat acaccgaatc
    41281 gaaacaagac gagattgtca ttgattttga tggtaatgaa gtctcttacc cccgtaacga
    41341 atggtacaag attcgcttgg cctatgccat gagtattcat aagtctcagg gaagtgagtt
    41401 cccagttgtc atcctaccga ttactagtgc tagccgacgt atgctggagc gaaatctcat
    41461 ctacacagcc attacacgtg ccaaaagcaa actcatctta ctaggcgaat tacaagcctt
    41521 cgactatgct acccaacaca tcggaactgc ccgaaaaacc tatctgattg aacgcttcag
    41581 tgatctactg gagaatgttg aagaaaagca acaagctgtc tctgaaacag tcacatcaag
    41641 tgcctctgaa caatcctaca tcctaaccga agaaaactgg gacagcattc cagccatgat
    41701 tgggattaca gacacagacc tcaaagagat ttttggaaaa tagtgcatca gaaaaaccac
    41761 caattcaggt gggttttctg tttattaaga ttattttact gttgctgtgc ttgtaatcaa
    41821 ttcaacagct tttttgatag tgatatcatg tttcaacatg tcagctgaaa gcaagttttg
    41881 aacttgtgca acttccatgt tgtagtctgc tgccaattgc tcaacttctt tttggatttc
    41941 ttcttctgaa gcatcaaatc cttcagcttt ggcaactgct tcgataacaa ggttagtctt
    42001 agtacgtgac tcagcttctg cttggtattg gttgtgaagg tcttcttgag tagttccagt
    42061 gatttggaag tacatgtcag ggttgatccc ttgacgttgc aagttcccaa ggaattcatt
    42121 tactgaacgg tgaacttctt cgtggatcat ttcttctgga agttctacga tttcagcatt
    42181 ttctacagct gtatcaattg ctgcaccttc aactgcatct ttgtaagctt cttctttagc
    42241 agtagccaat tctttgcggt atttttcttt caagtcagca agtgtttcaa cttcttcatc
    42301 aatgtctttt gcaagttcat cgtcaagagc tggaacttct ttagctttta cttcgtggat
    42361 agttgtcacg aatttagctt ctttacctgc aaggtcttct gcttggtagt cttctgggaa
    42421 tgttacgata acatcaacgg tttcgccagc tgagtgacct accaattggt cttcgaaacc
    42481 agggatgaat tgacctgaac caagtccaag tgagaagttt tcaccttttc caccgtcaaa
    42541 ttcaacaccg tcgatagaac caacgaagtc gatcacaaca gtgtcgccgt tttcagcagc
    42601 agcttccttg ataaccaatt cagccagatt gttgcgttcg cgttcgatac gctcttcaac
    42661 atcagcgtca gttacttctt tttctacatc aactgatact tcaaggtttt tgtagtcacc
    42721 caattttact tcaggttttg taacgacttc agcagtgata acccagtctt gacctttttc
    42781 cattgaagtt acgtcaattt ttggttgggc aaccacttca agaccagctt cttttacagc
    42841 tgcttcataa gcgtttggca aaagagcgtt cataacgtct tggtaaagtg attcttcacc
    42901 aaatttttta tcgaagatag gacgtggaag gtgaccttta cggaaacctg gaacattaag
    42961 agatttcttc actgacttga agacacggtc caattctggt ttgatttggt cttgagagat
    43021 agtgaaagtc aagacaccac ggtttgtttc tttgttttca aatgatacag acattctgtc
    43081 atttctcctt aaaatttttt aaatacagtc tattataaca ttttctataa ataaaatcta
    43141 gactaatgca gccaattata gacaacaagc ctttttccta actaaaacac tcataaagta
    43201 tagacaattt tagactactt tggatatctt ttggttagtc attttccaaa actaatttat
    43261 ctaatttttc agttatatag tagattgaaa ctagaatagt acacctctac ttctaaaaca
    43321 tttttagaaa tcgatttgac tgtcctgatc gatttgtcct gttcttattt cattttacta
    43381 tatagatcgc attcgaaaga attcgttcca aaattgcttg gttcgttctt ctcttggacg
    43441 actaaagaat tgatgggcat tatttttttc aacaattttc ccactgtcca taaaaatagc
    43501 atgatgggct acatctctag caaaacctat ctcataaagc tgatcaaaaa ctcaatctga
    43561 ggagaaaatc caataaggct ttccataata aaggacatcc tatcaagatt tttgaacacc
    43621 cgataggctg cctttttccg attttgaaga ctttttgtcc agcttctttt accttataaa
    43681 taacaacaaa aatcaaagac aagctagaaa gattaccgca gataactcaa acagtgtttt
    43741 gaggttgcag ataagactga cggagtcagt aacatatgcc taagacaaag cgatgctaac
    43801 ctgatttgaa gagattttcg aaaaatatta aatatactct acaatttgat caatcaattc
    43861 ttggtcgtct aaacctgtaa cttgtataac aacatctttg actgattgtt tagccaacaa
    43921 ttcacctaat cgaatacttt cttcatcatt tacatcgcga tagtcaaagg catagccaac
    43981 tgttttaagt aggtttttat atgacaaact gagttctttc aattcacgta tcggccggat
    44041 gaatcgttca ttatagccta attttcggat tggagtacga gctacgcgac taacctcgtc
    44101 cactatgaaa ggattttcaa accgttctat aatgactttg tgataattct ccaattcttt
    44161 tttatcaaag ttccatttgg caatcaagag actccgaatt tcagctaata cagattcaat
    44221 ccgagatttg atattaggat tttgaagagc ttccaaaatt gtcttggcac cataatgcgc
    44281 accaatgtaa gctgaagttg catgtccaga attgactgaa aaaagttttc gctcaataaa
    44341 gggttctaaa tcttcttcat aatgcacatc ttttagacgt aaatctggat ttttaagacg
    44401 cttggtttcc acgacccatt cattaaaggg ctcgaccaca acaaaaaggg aatcttcgtg
    44461 actttgtgct ggaacaatcc tgtctactgc agcatttgga aaacctatgt agttatcagc
    44521 aaatgtcaaa ccttccggac ttaaatattt cttgacttct tgataaagaa attgagaccc
    44581 gccaatcata ttttcacagg ccataacatc caatacctgt gtattccctg caactcggcg
    44641 agcttcgatt cctttggcta gaagttcagc gataaaaggg agtatattag gtccgattgc
    44701 agtagtaata atatccgtct tttgaatcgc ttcaatgact tgctcaggat gttctttgct
    44761 attaatgcca gccacattag ttacttctat acgagactgt cctttctgtg caatttcaat
    44821 ttcatacttg cccttttcat ttagagcatg aattatctga ttattgacat ccacaaaatc
    44881 aatatggaaa ccatttttaa atagaatttc acctataaaa ccacgaccga tattaccggc
    44941 accaaaatga acagaatgtt tcattcaatt tctccttttc ctatttgagg gacatatata
    45001 ctagatggca aatgacgaaa ttcaattatt caacagcatc aaataagcgc aatacttcct
    45061 cttttgactg agcatcggct agtttaagaa cattatctac atctgcacag aagatagaaa
    45121 ttttctgaat aatttctaag tgttcattac caataccagc aataccaaaa agaaccgttg
    45181 ccacttgagg gttagataca ttcccaaaat caacccctct agggacttga acgactgtaa
    45241 taccagactt gaggacatcc ttttttgctg catctgttcc atgcggtatg gcgataaagt
    45301 tacccatgta aacagatagc tctttatctc gctcaatcat ggcttcaata tagtcttcat
    45361 taacatatcc accctcataa agaacttgcc cacaataaca aatagcttcc tccttgttag
    45421 aaaattgttt attaagctta atcaaatgtt tttcaagttt catattagtt ctccaatttc
    45481 ttaatttttt cgttaaaaat agtatttagc atctgataaa taatggattg atttcccgtc
    45541 ttgtaaatct ctgtataaag atgattttca ataatcgact gactaatagc tgtcattaaa
    45601 tctctcgctt cctcgctagc ttctttagac attagcatta gcagacacct tttgacctct
    45661 tcttcctctc gtttcattga caatgcggag ataggtagtt tgagttcaaa catagtaaaa
    45721 ctatttgttg ttactttgct agactgacta tgtaaaagca ccagaccagt attaggaata
    45781 gccataggac tattctggaa gtgagataac aattttcttg ttaggtaatc tctgtcactc
    45841 acattcttct gatattggat gatttgttct accgtgtgct caaatgattg attattctcc
    45901 aattgaacca actcgaattg actcaaaagc tgactactag atattaaata atcttgcaag
    45961 tcataacttt tctctggagc gatagcatca cgcgcgacaa ttgtacgatg tgcctgtaca
    46021 tactgaagtc gttcctgtaa ttcaagcaat tctttgatgg ttggaaaact agaaattaca
    46081 tcgatttcct gattagtcag cggcttggta gataaaatat aatcatactg actgagatca
    46141 atactgtggt aatctactag agactgaata tctatcaact ctacaaaagg agcaacactc
    46201 ttaattttac tgactaataa atctctagtg acccgacgtt catccgttaa aagcaaaaca
    46261 cggactggat aaatctctgg actacggcct aagctagaga taaaatgtag ggcaatcatg
    46321 ccatactcat actctgtatg aagatatttc ggaaaaatat cattcactaa aagactgatg
    46381 actgtataaa gaaatttatt cctttcaact aaaatctgga cagattctgg caaattttca
    46441 ccctgaaaaa ggatagggac gcctaaactg agccgaatat gatggaaaag aaaattgaat
    46501 aaaaccttgt ccttaaaaaa gtcaatcttg gtatacatgg aaaccgtatc aatcagattt
    46561 gaaatattgt agaaaaattc accatcaaat ttctccgtaa agagcggatt gtcctgacgt
    46621 ttaatgatta attcatccaa gatgctcgca aaatagataa tttcctgaat actataaaat
    46681 tgtgcagtct gcttagagta agtttgaaaa attttttgag aaatttccaa agcctgctta
    46741 ctagtattag gtgaattttc aatgttttgc tcctgaccta taagagataa caagatcgca
    46801 aaaaacatct tcatcctagc atccatatct ggaaaacctg acagttgctt attaacaatc
    46861 tgactggcca gcctagttct atctgcttct aaaatatcaa agctcccaaa attaccggtt
    46921 gaaaaatctg ctactgagat acagtttgtc agtaaaatag ccaaaaatct tctcttacca
    46981 actgaatcac cagaaatccg ataacctttt tgtcgttcaa ttttcagatc aaagtctaaa
    47041 agacgcttat caatatctga aatatcctga ataatagtta cattactgac tttcgtgcac
    47101 tcttgcaatg cttcattggt gacaaaaccg ctctcagtca gaaggcgata ggtaatcaac
    47161 tcttgtcttt cttggggact atactcaact aacacttcaa gttctgtcgg caaatcatcc
    47221 aactctccag tcaaaatata gtatttccca tgcttcgtta tttcgattcc atattgctcc
    47281 atgctatctg tcaaatctga taaagttcga taaattgttc tagatgaaac ctgtaagatt
    47341 tcagtcatat cttgcattga aagcttccct acatgtagga aagccttcaa taattgttct
    47401 tctcgtttgg ttaatagcat tcgtaattta gaaacctcta gttctatttg tacattctag
    47461 cagccatttt gtcatattct ggtgttgtta ctaaactatc cacaataaga aattgagaat
    47521 tcggtgctgc ttgctgcact tctgcctgta aagaaatagt tgttacaatc attatgtttt
    47581 tagaattaaa ttcacctaat tctgaaattt tggcagtaga aactggaata cgaatattct
    47641 tgtttctaaa gatagcccga atcgtttcac agcccatagt tgcagttccc tgtgctttac
    47701 cataagcaac tactacagca tcaatatggt taaggtcaat ttcctgacta tctactggtg
    47761 ctgaagttgg tatatctcct tcaatttctg ctattggaaa agctcctgtt aatgaagcta
    47821 caatttcatc ataacgaggg gacgctaaga aattatcaac agaaacatga atagcacttg
    47881 gactcttgtc tttagctctt ggtgtcagtt cttcctgagt aacaattaat gtttttggtg
    47941 tatcaagcaa atttgagatt gcctgattag atactggaat ctctagacct gcttttttaa
    48001 ccttatctcg aagaatacta gctcccatag cagagcttcc cataccagca tcgcaggcga
    48061 aaatgatttt ttccactgag tctgtcgaaa caactgcatc aacagaagtt gatactaact
    48121 gacctttaga ctgagcctta gctgcttggg tagccgcctg agcagcttcg agcgaatcct
    48181 cagttgactt gtctgcatga agaataaggg ctgctacaag gaaagaaaca actgctgcca
    48241 ctaaaacacc taaaagaaca tttagatgag gccaaacacc ttttggcgcc gtagccataa
    48301 tcgcaataat agaacctggt gaagctggag atttaagacc agcgtctaag agttgaaaag
    48361 taaaagttcc agagatacct cctgccatag cagctaaaaa tagagtaggc ttcatcataa
    48421 cataaggaaa gtaaatttca tgaatccctc cgaagaaatg aataaccatt gccccccaag
    48481 aagaagattt agcagaacct ttaccgaata cagcataagc taatagaatt cccaggcctg
    48541 gtccaggatt agcttccaat aggaagagaa ttgacttacc agcttgagct acctgttcta
    48601 ctcccagagg agtaaaaatg ccatgattga gggcattatt gaggaaaagg actttagccg
    48661 gttcgatgat aatattagcc ataggaagga ggcgagcatt gacaatagcc tcaacaccat
    48721 tcccaacagc tccagtaaga gtcgatacga ctggaccgat tgcgtagaaa gccaaaagca
    48781 ataatgcaaa accaacgaga ccagctgaga agttattaac taacatttca aatccgggac
    48841 gaattttttc ctggaacttc tcatcaaatt tcttgatagt ccatcctccc agtgggccca
    48901 ttaccatagc tccgataaac ataggaacac tagaacctgt gattgcacca acagtagcaa
    48961 tagctcctac aacggcacca cgttggccat ggatcatata tccacctgtg taaccaatca
    49021 ggattggcaa taaatacgtt aacataggac caacaacagt agctaactgt tcatttggca
    49081 gatagccatc agcgataaag agggcagtca atactcccca agcaataaat gctccaatat
    49141 tgggcataac catatttgaa agcgatgtcc ctagtttttg aaccctgact ttcaatgata
    49201 ctttttcttc cattagatca tcctcctttt cttaaaacta gtatatacaa agttaaaaac
    49261 ctttaaaact aaagttttgt cacagatttt gtgtcaaaac attacttttg acgaggctgg
    49321 gcaaaaaggc ttaaaaatta taaaacgcat agtataggat gttgaaatga tgaactgcac
    49381 cccaaaagtt agacagaaaa aatctaactt ttggggtgtt tttatcatga a
//
LOCUS       DAVBCW010000004        48492 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVBCW010000004 DAVBCW010000000
VERSION     DAVBCW010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 48492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48492
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.004"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(72..1208)
                     /gene="lctO"
                     /locus_tag="VQ514_000181"
     CDS             complement(72..1208)
                     /gene="lctO"
                     /locus_tag="VQ514_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HET2499009.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     gene            complement(1833..3323)
                     /gene="lysS"
                     /locus_tag="VQ514_000182"
     CDS             complement(1833..3323)
                     /gene="lysS"
                     /locus_tag="VQ514_000182"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HET2499010.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLITKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKTIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(3451..3597)
                     /locus_tag="VQ514_000183"
     CDS             complement(3451..3597)
                     /locus_tag="VQ514_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HET2499011.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAGDTF
                     TSFQ"
     gene            3823..4491
                     /locus_tag="VQ514_000184"
     CDS             3823..4491
                     /locus_tag="VQ514_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2499012.1"
                     /translation="MVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHYRILV
                     LAQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSYMAESFR
                     SGLEAISQTQQEIGLAIGLTPLQVFYYVVLPQATAVALPSFSANVIFLIKETSVFSAV
                     ALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRLRHAGFGNPST
                     LSRK"
     gene            4451..5128
                     /locus_tag="VQ514_000185"
     CDS             4451..5128
                     /locus_tag="VQ514_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2499013.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIEISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLDFTRIMPQLVLLFIVYFGLARNFNINISGETSAIIVFTLW
                     GAAEMGDLVRGAITSLPKHQFESGQALGLTNVQLYYHIIIPQVLRRLLPQAINLVTRM
                     IKTTSLVVLIGVVEVTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVCYPISKLSTH
                     LEKHWRN"
     gene            5129..5886
                     /locus_tag="VQ514_000186"
                     /pseudo
     CDS             5129..5886
                     /locus_tag="VQ514_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            5898..6759
                     /locus_tag="VQ514_000187"
                     /pseudo
     CDS             5898..6759
                     /locus_tag="VQ514_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
     gene            complement(6839..7486)
                     /locus_tag="VQ514_000188"
     CDS             complement(6839..7486)
                     /locus_tag="VQ514_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2499014.1"
                     /translation="MNEIIILKNIELKLKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(7483..8181)
                     /locus_tag="VQ514_000189"
     CDS             complement(7483..8181)
                     /locus_tag="VQ514_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499015.1"
                     /translation="MGKLSSHMWRLNKIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLVLFCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDVGGDLFLQLLFLVFLFQ
                     SIFYFTRQKRRFIE"
     gene            complement(8191..8943)
                     /locus_tag="VQ514_000190"
     CDS             complement(8191..8943)
                     /locus_tag="VQ514_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499016.1"
                     /translation="MKQFVQFYKKDFLSVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTATLFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIGFFFSY
                     IAYYFFLSLLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLNNGIIGLLPIFQYMV
                     NSNPYALIYWLTLLSIIIPLTVFSVHRNWRRV"
     gene            complement(8943..9707)
                     /locus_tag="VQ514_000191"
     CDS             complement(8943..9707)
                     /locus_tag="VQ514_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499017.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKKYLAIVG
                     STAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQLLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKIGAIFIPVAYMMVGGIFWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            complement(9803..10206)
                     /locus_tag="VQ514_000192"
                     /pseudo
     CDS             complement(9803..10206)
                     /locus_tag="VQ514_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10386..11018)
                     /locus_tag="VQ514_000193"
     CDS             complement(10386..11018)
                     /locus_tag="VQ514_000193"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HET2499018.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYRELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            complement(11052..11753)
                     /gene="pyrF"
                     /locus_tag="VQ514_000194"
     CDS             complement(11052..11753)
                     /gene="pyrF"
                     /locus_tag="VQ514_000194"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HET2499019.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKVLSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQNWN"
     gene            12074..12166
                     /locus_tag="VQ514_000195"
                     /pseudo
     CDS             12074..12166
                     /locus_tag="VQ514_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516271.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(12488..13201)
                     /locus_tag="VQ514_000196"
     CDS             complement(12488..13201)
                     /locus_tag="VQ514_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2499020.1"
                     /translation="MLKRFLALVWLRCQIILSNKSILLQVLVPFAFTYFYKYLMETQG
                     KVNDQQALVLLMMCLPFSLALAVGSPITIILSEEKEKYNLQTLLLSGVKGSEYILSTM
                     FLPFLLTFVIMGTTPLILGVTIVHTFNYITIVLLTSLSIILFYLLIGLTAKSQVVAQV
                     ISLPAMILVAFLPMLSGLDKTVAKITDYSFMGLFTKFFTKWEGFSWNKTLIPNLTLLI
                     WIVLLLTLITITIRKKKIS"
     gene            complement(13194..13889)
                     /locus_tag="VQ514_000197"
     CDS             complement(13194..13889)
                     /locus_tag="VQ514_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2499021.1"
                     /translation="MTGQFLADKGQSIILGQKSQNLTSGELKRIGLVSDTSGFYEKMS
                     LYNNLLFYSKFYNISKSRVDNLLKRVGLYDSRKMVAGKLSTGMRQRMLLARALINKPA
                     VLFLDEPTSGLDPTTSRTIHELILELKTAGTTIFLTTHDMNEATLLCDYVALLNKGKL
                     VEQGAPSELIQRYNKDKKIKVTDYNGNQITFDFTSLEQVSQADLENIFSIHSCEPTLE
                     DIFITLTGGKLNA"
     gene            complement(14229..15561)
                     /locus_tag="VQ514_000198"
                     /pseudo
     CDS             complement(14229..15561)
                     /locus_tag="VQ514_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812020.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
     gene            complement(15545..16343)
                     /locus_tag="VQ514_000199"
                     /pseudo
     CDS             complement(15545..16343)
                     /locus_tag="VQ514_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824573.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M50 family protein"
     gene            complement(17133..18332)
                     /gene="divIB"
                     /locus_tag="VQ514_000200"
     CDS             complement(17133..18332)
                     /gene="divIB"
                     /locus_tag="VQ514_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HET2499022.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEADKEKEEKEEPES
                     KEKEEQDKKLSKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVVDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEESNRNQT
                     TQRSSRR"
     gene            complement(18342..19400)
                     /locus_tag="VQ514_000201"
     CDS             complement(18342..19400)
                     /locus_tag="VQ514_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HET2499023.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVCWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLSKVEHVGA
                     VAKVSDQKNPEPDELVDIQSHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQLENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            complement(19402..20754)
                     /locus_tag="VQ514_000202"
     CDS             complement(19402..20754)
                     /locus_tag="VQ514_000202"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HET2499024.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLTSKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRGVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            complement(20858..21499)
                     /locus_tag="VQ514_000203"
     CDS             complement(20858..21499)
                     /locus_tag="VQ514_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2499025.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTFL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNCVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTTSLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            complement(21501..23522)
                     /locus_tag="VQ514_000204"
     CDS             complement(21501..23522)
                     /locus_tag="VQ514_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HET2499026.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFL
                     GGGVVLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLSYKALVPVIKGR
                     LPLKRLMILTLLCQLVAVFTVGYAVKTGLMSYQRLKELEISKQAWQDRADYYQISFGL
                     GDRGKDTENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANT
                     LYVSPSYLDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYG
                     KSGEEASAPLDYEMKAHVSYLSMGEKRFVYNNGENPVSTQYLTDPILVVFTPTSTGDS
                     FISLSSWSINAGKQLFIKGYESGLELLKKAGIYEQVSYLKEGRSVYLTRYNEVQTETA
                     TLILGAIVGIASSLLLFYSVNLLYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFV
                     FGASLFILSSRDLVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            complement(23593..23892)
                     /locus_tag="VQ514_000205"
     CDS             complement(23593..23892)
                     /locus_tag="VQ514_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HET2499027.1"
                     /translation="MKTKKHRLLALALISSFTLLGAASAAVQYPDGGVWTYGEGSGGG
                     WAFSNYYHGKKYHYSSLVSRWNSHSDKGEASAGKTSYAWIWTKWGEQVAFYCDYD"
     gene            complement(24258..24512)
                     /locus_tag="VQ514_000206"
     CDS             complement(24258..24512)
                     /locus_tag="VQ514_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HET2499028.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
     gene            complement(24532..26373)
                     /gene="typA"
                     /locus_tag="VQ514_000207"
     CDS             complement(24532..26373)
                     /gene="typA"
                     /locus_tag="VQ514_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HET2499029.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            26535..27260
                     /locus_tag="VQ514_000208"
     CDS             26535..27260
                     /locus_tag="VQ514_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HET2499030.1"
                     /translation="MRLDNLLAQEKVSRKAMKQALLRGEILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVQVNELLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSLSQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLA
                     ESNYRPLNQKELQIIKNYLEMSR"
     gene            complement(27300..27527)
                     /locus_tag="VQ514_000209"
     CDS             complement(27300..27527)
                     /locus_tag="VQ514_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499031.1"
                     /translation="MKEKKKNPLFVGILSIILGLLFPIVGLILGIIGLVLAISYQKES
                     QLDYKIEKILNILGIVISVVNWIVAIALIFR"
     gene            complement(27616..27996)
                     /locus_tag="VQ514_000210"
     CDS             complement(27616..27996)
                     /locus_tag="VQ514_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET2499032.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            complement(27996..28265)
                     /locus_tag="VQ514_000211"
     CDS             complement(27996..28265)
                     /locus_tag="VQ514_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HET2499033.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            28329..29237
                     /locus_tag="VQ514_000212"
     CDS             28329..29237
                     /locus_tag="VQ514_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET2499034.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            complement(29291..30046)
                     /locus_tag="VQ514_000213"
     CDS             complement(29291..30046)
                     /locus_tag="VQ514_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET2499035.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEADAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(30067..30996)
                     /locus_tag="VQ514_000214"
     CDS             complement(30067..30996)
                     /locus_tag="VQ514_000214"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HET2499036.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTDASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            complement(31011..31634)
                     /locus_tag="VQ514_000215"
     CDS             complement(31011..31634)
                     /locus_tag="VQ514_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HET2499037.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTEQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGFGRIFSENEEKISDDLLFEIAQIFSHQLVFEESGKIYMKPVKDEEVVSLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            complement(31627..32865)
                     /gene="hflX"
                     /locus_tag="VQ514_000216"
     CDS             complement(31627..32865)
                     /gene="hflX"
                     /locus_tag="VQ514_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET2499038.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            complement(32858..33742)
                     /gene="miaA"
                     /locus_tag="VQ514_000217"
     CDS             complement(32858..33742)
                     /gene="miaA"
                     /locus_tag="VQ514_000217"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HET2499039.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKCFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEDIHSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQEILYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTLEEARESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            33863..34033
                     /locus_tag="VQ514_000218"
     CDS             33863..34033
                     /locus_tag="VQ514_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HET2499040.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            complement(34081..34920)
                     /locus_tag="VQ514_000219"
     CDS             complement(34081..34920)
                     /locus_tag="VQ514_000219"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HET2499041.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPIITLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(35017..35976)
                     /locus_tag="VQ514_000220"
     CDS             complement(35017..35976)
                     /locus_tag="VQ514_000220"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HET2499042.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENTFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            complement(36103..37101)
                     /locus_tag="VQ514_000221"
     CDS             complement(36103..37101)
                     /locus_tag="VQ514_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HET2499043.1"
                     /translation="MNKRLFLKMSLVTLPILALFSQPVLAEENIHFSSCKEAWANGYS
                     DLHEGEPGYSAKLDRDHDGVACELKNAPKGTFKAKQAATTQTDTTSSTASGWVKQDGS
                     WYYFDGNGNLVKNAWQGNYYLKADGKMAQSEWIYDSSYQAWYYLKSDGSYAKNAWQGA
                     YYLKSNGKMAQGEWVYDSSYQAWYYLKSDGSYARNAWQGNYYLKSDGKMAKGEWVYDA
                     TYQAWYYLTSDGSYAYSTWQGNYYLKSDGKMAVNEWVDGGRYYVGADGVWKEGQASTA
                     SSSNDSNSEYSAALGKAKSYNSLFHMSKKRMYRQLTSDFDKFSNDAAQYAIDHLDG"
     gene            complement(37151..37768)
                     /locus_tag="VQ514_000222"
     CDS             complement(37151..37768)
                     /locus_tag="VQ514_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET2499044.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSNYQQA
                     KERVYQHLAQETEPFVLIGLSLGAALTLELSSYDLPNLRALILSGCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKTLMVGVSEELKTLDLTDIAGTCSYPTLLICGSKDKPNL
                     SSMKALHRLLTDSQFQIIPDGPHVLNKAKPKEFVEKTRSFLELLK"
     gene            complement(37769..39490)
                     /gene="pabB"
                     /locus_tag="VQ514_000223"
     CDS             complement(37769..39490)
                     /gene="pabB"
                     /locus_tag="VQ514_000223"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HET2499045.1"
                     /translation="MHRKTVIDFRTLGERYTFAQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDSVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLSANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGEISQKNLLFED
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANRDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGELLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQYLLETGQVEEKVLTLADLTQAEAIYGCNAVRGLY
                     ELSLEEN"
     gene            complement(39559..45246)
                     /locus_tag="VQ514_000224"
     CDS             complement(39559..45246)
                     /locus_tag="VQ514_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HET2499046.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASVFLVGAMGLVVLPSAGAVDPVATLALASREGVVEMDGYRYVGYLSGDILKTLGLD
                     TVLEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEETPKEEAPRTEESPKEEP
                     KSEVKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKSEEKVAVKPESQPSDKPAEES
                     KVEPPVEQAKVPEQPVQPTQAEQPSTPKESSQEDNPKEDRGAEETPKQEESTPDTKAE
                     ETVEPKEETKTAKGTQEEDYVGEALVPEQLPEYKVTEGTLVEESTTELDYKTETTEDP
                     TKYTDEETVLRNGEKGSQVTKTTYKTIGGVKTDQVLSTSTEVTKEPVNQQVIRGTKPI
                     EGTTVETREEIIPFETKEQEDDTLKRGTRQVTQEGVNDKKQITETYKTIRGEKTSDAP
                     TITETVIEKPQDKIIKNGTKELEKPTLEWAKTDKDVLKKSATASYTLTKPAGVEIKSI
                     KVALKDNTGTVVKEVTVEENNLQATLDNLKYYQGYTLSTTMVYDRGNGEETEILEDKE
                     VQLDLKKVEIKDIKETRLMKVDENGNETDSSLLETVPENLTSYYLKITTNHNKVTRLA
                     ITNIEEVTEEETSLFKVTASAPDLIQRDSNNSLSQTYTYYIEKPKASQNNVYYNFKDL
                     VDAMQKNPNGEFKLGSDLNATNVPTPSKSYVTGKFTGHLTSVDGKHFSIHNTARPLFD
                     RVEGGSIKNINLINVNINMPWANQLASLAQTVKNATVENIKVTGTIVGNNNVAGILDK
                     LDDAGIMTNVAFIGKVQSVGDKGWNLAGIASEIYRGNLNKAYADVELKGLNTRAGAIV
                     GHLAHDFNPRDVGAWSNVRNSVAKGTIDVARGDVGGLIGKDWPWGTIDNNISLVNVKR
                     GDIFFGSNDMDDEFVSNWIKPNNATVIGVSSGDKTHKQSSKIKEISQVEADKKIKDMG
                     ITADTFEVTEHLVDKLNRRILRDEEYKTIQDYNANRELVYRNVEKLQPFYNKEWIVNQ
                     GNKLTTDSPLMNKEVLSVTAMKGNDFITDLTDADHIMVHYADKTKDIFTISPKDSQVK
                     QVKEYSVAELGEVVYTPNMVVKDRADLISAIEGILSPIELQSDPIYQRLGRTGDNRVN
                     AIKNLYLEESFKEVKDHLNTFVTKLVENEDHQLNTDEAAKRALIKKIEDNKAAVLLGL
                     TYLNRYYGVKFDNFNIKELMLFKPDFYGKNVSVLDRLIEIGSKEDYIKGSRTQESFRE
                     VVAKNTLSGNLHDFLTYNMKLFTNETDINVWFKKAIENNAYVVEQPSTNPAFANKKYR
                     LYEGLNNGQHGRMILSLLNLKDAHLFMISTYNTISFSSFEKYGKDTEEKRESFKSEIN
                     KRAKEQVNYLDFWSRLATDNVRDKLLKSQNVVPTPVWDNHGAPGGWPDRFGHRKDKPD
                     YAPIREFFGRIDKYHDYKYGYGAYAYIFADPQPMDAVYFVMSDLISEYGTSAFTHETT
                     HVNDRMAYLGGHRHRQGTDLEAFAQGMLQTPAEHGHQGEYGALGLNMAFERSNDGNQW
                     YNPDPTKLQTRDQIDHYMKNYNEAMMMLDYAEAEAVLPKVKGDNSKWFKKIDRETRRP
                     MDRNKLSGPHQWDKVRDLIDAEKTTNLETIDDLVNNNFMTIHGNPGNKVFHPEDFGTA
                     YVNVNMMAGIYGGNTSQGAPGSLSFKHNAFRMWGYYGYEHGFIDYVSSKHQGAANKEN
                     NGLLGDDFIIKKVSNNQFSNLEDWKKQWYGEVLTKSKKGFAPIEINGTQISTYEQLKT
                     MFDEAVTKDLTELNDSSIKDKYRNTVVLKSKVFKALLKNTDGFSGDLFTAPQA"
     gene            complement(45268..45831)
                     /locus_tag="VQ514_000225"
     CDS             complement(45268..45831)
                     /locus_tag="VQ514_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HET2499047.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGVLFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILGSRGIHLAVQGCEHVNRALVVERQVAEQFDLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKS"
     gene            complement(45947..47638)
                     /locus_tag="VQ514_000226"
     CDS             complement(45947..47638)
                     /locus_tag="VQ514_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2499048.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIETGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYQMKIDSRPQKGTKVEIYINRIETS"
     gene            complement(47635..48372)
                     /locus_tag="VQ514_000227"
     CDS             complement(47635..48372)
                     /locus_tag="VQ514_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2499049.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
BASE COUNT        14570 a        10465 c         8483 g        14974 t
ORIGIN      
        1 ttttcgatag caatttattc ttccaataca cgaagaaaaa cctccacatt cagtggaggc
       61 aatctgtttt atcaatacaa ttttaagtca cgagggtcaa ctgggaaggt tgggttgtat
      121 ggattgtgac ggagcttaaa gtgtttgaca tcttcaatgg tctgagctcc agacaattgc
      181 ataactgtct tcaattccgc attcaagtgt tcaaagactt gacgcacacc gacactacca
      241 ccgagagcca agccataaat aacagggcga ccaatagcta ccaagtctgc tcctgaagcc
      301 aaggctttaa agacgtgttg accacgacga acaccagagt caaagacaat cggcacacgt
      361 ctatcaactg cttctgccac ttcttgaagc gagtcaaagg cagctggtcc accgtcgatt
      421 tgacgaccac cgtggttggt tacccagata ccagaagctc ctgcagcaag tgaacgttca
      481 acgtcctcac ggcattgtgg tcccttgaca tacacaggaa gaccagagta ttcagcgata
      541 aattctacat cgcgtggaga caagcgttgt ttagctgatt tgtaaacaaa gtccattgat
      601 ttaccagcac cttctggcag gtattcttca acaatcggca tgccaactgg gaagacaaaa
      661 ccattacgct tatccacttc acgattcccc cctacagtag catctgccgt caagacaatc
      721 gctttataac cttcagcctt cacacggtcc atgatgtggc ggttgatacc gtcatcctta
      781 ctaaagtaaa attggaacca atggggtgtc ccttgaaggg cttcagaaat ctctggaagg
      841 tcaacagtag agtaagagct ggttgtataa agagaaccaa actcatgcac accacgcgca
      901 gtcgccactt ccccctgttc atttgccaat ttatgagccg caacaggcgc cataatgatt
      961 ggtgaagata gtttttcacc tgcaaattca atctctgtac ttggattttc aacatcgcaa
     1021 agtgtatgag gaacgatgag tttgtggtta aaggcgcgga tattctctct taaagtgaaa
     1081 gtatcttccg ccccactagc gatatagcca aatgctgctt taggaataac ttgttgcgcc
     1141 attggctcca aatcataggt attgatgaag tctacatgac cttctgcatt gcttgttttg
     1201 tatgacataa aatgccctcc ttgattaagt aagcgtttgc tttttactac acaactataa
     1261 taactctttt ttcaatcatt ttcaagagtt ttgcttaaaa accatcttat tttttcatac
     1321 aatatactag ccctactttc cttaaactct aaactttagt ctatattaag tgaatcgttg
     1381 agattttttg ctgccaggca tttgaactac ttttttattt gcggattcta tctactaata
     1441 ctataaaaaa acaagtgaat aagacaccag aacatcctct aataggacct atggacttat
     1501 atcagtccca tatatactgt ttaagcaaga tactatccta gttgacgcta acttcctatc
     1561 ccacttatta attttttatt tatagtgaat ctgaacaaat caattgggga atccaagtca
     1621 atttctagta ctttttgaac agtttcagcg tactatcgta aaatgaaata aaacatgcac
     1681 aaattgctta gggaatttaa accaatttct aacaatgtct tagaaatcaa agtgtactat
     1741 ttcaacttca atatactaca cttcattttc tccttaaatt cagtctcttt ttgaatcaaa
     1801 aaatcccctg gtaagagcca gaggataaga atttatttca ttgttgggaa gagcaataca
     1861 tcacggatag ttgttgtatc agtgaggagc atgcagagac ggtcgatacc gattcccaaa
     1921 ccacctgttg gtggcatacc gtattcaaga gcttcaatgt agtcatagtc gatgcctgtc
     1981 gcttcatcat caccaagttc tttggcttta gcttgggctt caaaacggct aagttggtcg
     2041 attgggtcgt tcaactcagt aaaggcatta ccgtactcct tagtcatgat aaagagctcg
     2101 aaacggtcag taaagcgttg gtcttcagga tttttcttag cgagtggaga tacagctact
     2161 ggatgtccat agacaaaggt tggttggatt aaagtttctt caacaaactc ttcaaagaag
     2221 gcattgatga tgtgaccaac ctcagtgtag tgtttctcaa ctggaacttt cttctcagca
     2281 gcgatagttt tagcttcttc caaagtcatg tcttgccaga aatcgacacc agtaatttct
     2341 ctgatagcat ccaccatatg aacacgctta aatggttcgt taatcttgat ttcagtacct
     2401 tggtagttga ctgggccatc acctttgact gatttagcag cgtgttggat aatgccttca
     2461 gtcaagtcca tgatgtcttg gaagtctgca taagcttggt aaacttcgat agaagtgaac
     2521 tcagggttat gagtagcgtc cattccttcg ttacggaaga tacggccaat ttcatagaca
     2581 cgttccatac cacccacgat aaggcgtttc aagtgaagct cagtcgcgat acgaagcacc
     2641 atgtcaatgt tttgggcatt gtggtgggtg ataaatggac gggcagcagc accaccggct
     2701 tcattatgaa gaacaggtgt ttccacttca aggaatcctt tttggtcaag gtaacgacgg
     2761 atttcagaga tgatttttga acgagtgaca aagcgttcaa agctttcacg attagaaatc
     2821 aagtcaaggt aacgtttacg gtaaattgtt tcaacgtctg tcaaaccatg gaatttctca
     2881 ggaagaggac gaagagcctt agacaagtgt gtgatgtggg ttgccttgat agagagttct
     2941 cccatatccg tacgcatcac ttcaccttcg acaccaagga agtcaccaag gtctgctttt
     3001 ttgaagattt cgtagttttc ttcaccgaca gcatccttac gaacgtagat ctgaatctgg
     3061 ccttcgcggt cttgaaggtg ggcaaaacca acttttcctt taccacgttt ggttatcaag
     3121 cgtcctgcga tagtagctgt ttcgttttta tcgtgtaatt gttctttatc gaggttggca
     3181 tatttatctt ttaattcttg tgaatttgca gtacgttcaa aacgttttcc gaaaggatcg
     3241 attccttgtt cgcggagcgc agccattttt tcacggcgaa cgatctgctg gtcatttagt
     3301 tcttccatat gttctgtaga catgggatcc tcctagtttt actcaaaatt gaatacgatg
     3361 agtcattttt tgcgatactc ccattttatc ataaataagc aagaaattca cggataatct
     3421 ttaaaaacta aaaagaacct gacgctaaaa tcactggaaa gaagtgaaag tatctcccgc
     3481 cccactagcg atatagccaa atgctgcttt aggaataact tgttgcgcca ttggctccaa
     3541 atcataggta ttgatgaaat ctacatgacc ttctgcattg cttgttttgt atgacataaa
     3601 atgtcctcct taataagtaa gcgtttactt tgtgtattac aaaaatatct taactctttt
     3661 tcaaaacttt taaaatattt tgtttggaaa tttcagaaat tttatgtcta tgataaaaat
     3721 ccttataacg gcaataaaaa atagatatta tccaaagaag attttaagtg ctacaataac
     3781 tgtattattt ctagatggga ggttctattt ttggattgat ccattgttga acaatatcta
     3841 ccactatatc aaaaggcatt ctttctgacc ttgcatattg cagtttgggg aattttggga
     3901 tcctttctgc tcggtttaat cgttagtatc atccgacatt atcgaatcct tgttttggcg
     3961 caagtagcga cagcctacat tgaattgtca cgtaatacgc cccttttgat tcaactcttc
     4021 tttctctact tcggtcttcc ccgaatcggg attgtcctat cttcagaagt ctgtgcaacg
     4081 cttgggcttg tctttttagg aggctcctat atggcagaat ctttccgaag tgggctggaa
     4141 gccatcagtc aaacccagca ggagattggc ctcgctattg gtctgacacc tctacaggtc
     4201 ttttactatg tggttcttcc gcaagcaaca gcggtggcac tcccctcctt tagtgccaat
     4261 gtcattttcc ttatcaagga aacctctgtt ttctcagcag tggctttggc cgacctcatg
     4321 tacgtcgcca aggatttgat tggtctctac tatgagacag acattgcgct agctatgttg
     4381 gtagttgctt atctgatcat gctgctaccc atctcactgg tctttagctg gatagaaagg
     4441 aggctccgcc atgcaggatt cgggaatcca agtactcttt caaggaaata atctcctgag
     4501 aatcttacag ggattgggcg ttacgattga gatatccatc ctgtctgtcc tcttatccat
     4561 gatgttcgga acagtcatgg gaatcatcat gacctcccat tctagaatca tacgattttt
     4621 aacacgattg tatctggact ttacccgtat catgccccag ctggtgctac tcttcatcgt
     4681 ttactttggc ttagctcgaa actttaatat caatatctca ggtgagactt cagctattat
     4741 cgtttttacc ctctggggag cagctgaaat gggagacttg gtacgtggag ctatcacttc
     4801 tctccctaaa catcagtttg aaagtggaca ggcactcggc ttgactaatg ttcaacttta
     4861 ctaccacatc atcatcccac aagtcttaag aagactgcta ccgcaggcta tcaatcttgt
     4921 cactcggatg attaaaacca cttcattagt tgttttgatt ggggttgtgg aagtgaccaa
     4981 agttggacaa caaatcatcg atagcaatcg cctgaccatc ccaactgctt cattttggat
     5041 ttatggaacc attctagtct tatatttcgc agtttgctac cctatttcca aactatccac
     5101 tcacttagaa aaacattgga gaaactaaat gtctgaaact atcttagaaa tcaaggaact
     5161 aaaaaaatcc ttcggagaca atcccatcct ccaaggactt tctctagaaa tcaaaaaagg
     5221 gaagttgttg tcatcctagg gccatctggt tgtgggaaaa gtaccctcct tcgttgcctc
     5281 aacggcttag aaagtattca aggtggagat attcttctgg atggtcagtc tatcgttgaa
     5341 aataaaaaag attttcacct agttcgccaa aagattggca tggtctttca aagttatgaa
     5401 ctctttcccc atctggatgt cttacaaaac ctcatcctag gccctatcaa agctcaagga
     5461 agggacaaga aagaagtaac ggaagaagct ttgcaattac tagagcgtgt cggtttgctg
     5521 gataaacaac atagctttgc ccgtcaatta tctggtggac agaagcaacg tgttgcaatt
     5581 gtccgtgccc tcctaatgca tccagaaatc atcctttttg acgaggtgac tgcttcgctg
     5641 gatccagaaa tggtgcgtga ggtgctggaa cttatcaatg atttggccca agaaggccgt
     5701 accatgattt tagtaaccca cgaaatgcag tttgcccaag ccattgctga ccggattatc
     5761 ttcctcgacc aagggaaaat cgctgaagaa ggaacagctc aagccttctt taccaatccg
     5821 caaaccaaac gagcccagga atttttaaac gtctttgact ttagccaatt cggctcatat
     5881 ctataaagga gattcttatg aaactattca aaccactctt aactgtttta gcacttgcct
     5941 ttgcccttat ctttatcact gcttgtagct caggtggaaa cgctggttca tcctctggaa
     6001 aaacaactgc caaagctcgc actatcgatg aaatcaaaaa aaaagcggtg aactgcgaat
     6061 cgccgtgttt ggagataaaa aaccgtttgg ctacgttgac aatgatggtt cttaccaagg
     6121 ctacgatatt gaactaggga accaactagc tcaagacctt ggtgtcaagg ttaaatacat
     6181 ttcagtcgat gctgccaacc gtgcggaata cttgatttca aacaaggtag atattactct
     6241 tgctaacttt acagtaactg acgaacgtaa gaaacaagtt gattttgccc ttccatatat
     6301 gaaagtttct ctgggtgtcg tatcacctaa gactggtctc attacagacg tcaaacaact
     6361 tgaaggtaaa accttaattg tcacaaaagg aacgactgct gagacttatt ttgaaaagaa
     6421 tcatccagaa atcaaactcc aaaaatacga ccaatacagt gactcttacc aagctcttct
     6481 tgacggacgt ggagatgcct tttcaactga caatacggaa gttctagctt gggcgcttga
     6541 aaataaagga tttgaagtag gaattacttc cctcggtgat cccgatacca ttgcggcagc
     6601 agttcaaaaa ggcaaccaag aattgctaga cttcatcaat aaagatattg aaaaattagc
     6661 aaggaaaact tcttccacaa ggcctatgaa aaaacacttc acccaaccta cggtgacgct
     6721 gctaaagcag atgacctggt tgttgaaggt ggtcattaac tcttaaaagg gactggattt
     6781 taagctccaa tcccttttta agattttacc tataacatcc tgagtctatc taagatgttc
     6841 aatctgaaca cagtgtacat actttatctt ctattgcata tactttatca cataagatac
     6901 gaatatcctc ttcactatga ctagcaatca aaattgttgt ccctttttca ctagagagct
     6961 ttctaaacaa tgttctcata ttttctacac ttgatttatc caaggcattc ataggttcat
     7021 ctagtaaaag aatagaggga ttctccatga ttgcttgagc aatccctagc tttttcctca
     7081 tacctagcga ataagtttta actttctggt ctttttgctc atatagacca actattttca
     7141 gtgtatcatt gatttcctga ttaccaacta ctcctcgtat gcttgccaaa tattgtaaat
     7201 tcttaaagcc actataataa tttataaaac caggttcttc aatcaaagct cccaaattag
     7261 ctggaatttt tctctcagga acaatatttt ccccattgat taacacttct ccataagacg
     7321 gactatataa accagctatt aatttaaaca atacactttt ccctgagcca ttcgcaccag
     7381 taattcctat aatttccccc tgtttacaac taaagttaag gttttgaaaa acacatgtct
     7441 tttttaattt caactcaata ttttttaata taattatttc attcattcta taaacctcct
     7501 cttttgacga gtgaaataga aaatgctttg aaaaagaaag actaaaaata gcaactgaag
     7561 aaacaaatct ccccctacat ccccattccc tcgattcaaa atataaaata gataattagt
     7621 tcgatttcct acaaatagac caccaaacac aatcatgagt aaaaagaaac taacgcaagc
     7681 aaagttcatt tgtacattcg ctcctagtgc gtataacata aagcaaaaca agactaacac
     7741 acaagcattc aaagaatcaa ctataaacaa aactcctata tcccagtttg gtaactgtaa
     7801 cataaattga atcactagaa cttttaccat gattaaaatg agtccataaa gaaaacaggt
     7861 ccaaatctga tacctaatcc agagcttaat agaagaaaat cgaatcactt ccataaagaa
     7921 atcggatgct cttctacgat atacaactgc tagaaaaaat gtatgaataa taaaccaatg
     7981 aagcaaccat atagataaga ctaaaaaaga gttttgactc agattaggcc cttttaaaat
     8041 ttctataaca agtctatcat ttccttctaa ccaataccat aatcctaaac aaatcaatat
     8101 ccaaaaaaga acataacccc aaaagtactt ggtatagatt atcttattca acctccacat
     8161 gtgactactt aactttccca tttccaactt ttacactctc ctccagtttc tatgaacaga
     8221 aaatacagtc aatggaatta tgatagatag taatgtaagc caataaatca atgcatacgg
     8281 attggaattt accatatact gaaaaattgg caataatcca attatcccat tgttcaactg
     8341 ataaatccag aataaggatt ctacaaataa aaaagtaaac agaaatacta agctcatgtt
     8401 tgatttttta aaccaagaaa aactgctaat agtaagtaag gataaaaaga aatagtatgc
     8461 tatataagag aaaaaaaatc ctataaagaa actagcattg tttgcaatac ttataccata
     8521 aaattctctt aaaaaatcaa cctcctttat ctccaaagag aagctaaaac cattactaaa
     8581 tgcaattaga aaaatcaata aaaataaagt cgccgtccaa atccccgtac taagagctgc
     8641 taatttgaaa ctaaaactgg taaagtgctt aattgatttc agacgaatgc gacactccaa
     8701 cctattaaaa tagttattca tcaaataaaa aaagaacaat atatatgtga acggaaagca
     8761 atatactcca gtcgtcatat cttgaagtaa aactaaaatc cattctaata catttggatg
     8821 gattgaatat tggcgacagc gcaataaata tactgtacta gataaaacac aggatagcag
     8881 taatataaaa taaaccaata ctgataaaaa atcttttttg taaaattgaa caaattgttt
     8941 cattatacat agtcctctga atgtagaaaa aatgtaccat aaacaaccaa acaactaaca
     9001 aataaaataa aagcaagatg cccactaact aaggaaagac tgatatcttt ctgatatccc
     9061 aaagctaatg ttgtcacagg ttctaagtaa gatagcccta aaatagccca aaaaatacca
     9121 ccaaccatca tataggcaac tgggatgaaa atagctccta tttttttctt cactagcaaa
     9181 gcactagcta gtccaaaaat agagaacaca gcgccccaaa ctccatacca gagagtcgtc
     9241 acaagactat agagcaactg attagaatca aataattctt ttaaggcacc actataatct
     9301 ccaatataaa tttcctgata aggagtcact aaaagattaa ttcctaataa taataaatag
     9361 gggagaaaaa agactagaaa agaagaaata attgcagtac tacctacaat agccagatac
     9421 ttctttttag aaattcggca caattgtgct gttagaaaat gactctcagc atcctctatt
     9481 atctgactag aatagggcag tgtacagata agtgcagcta ctaggctaat cggtgaaaat
     9541 cctcgaatag aagaagcggc aaaaaaaatc gataaacctt caattttata aataccattg
     9601 aaagcaagga aatttcctaa actcatgcaa agaagggtca aaaataaaga catataaaat
     9661 cgaggtgatt gaacgactcg tacaagatta caaatgaaaa attccatcct tactcctcct
     9721 tataataaaa atagggtgta gcattctttt ttcatgctac acccacaatc aaccatcttt
     9781 aaggcttact ctgacaagta agttaataag aatctggact ccaagaacct gaagtatgaa
     9841 ttcttacatg atttccaaat tgtggcgcca tagctaatct agtaccagaa ccaatataat
     9901 tgtcaccacc tccattatag tacatgacaa tcctagagcc agaccccaat gaatataccg
     9961 gggtaatatc tgacccacta taggcgctac gaatagaggt atttaacctt ttaccgccac
    10021 cagtgctgtc actattatta attccagcag aggcgttttc tttctcaagc tcactagttg
    10081 tatccgaacc ccaacgcggc aacgttaaat cataatttcc aatctgtcta gctaaaactg
    10141 tagggagaaa caaactagat gcaattgtaa ctacgcaaaa aaggaatata tttttcttct
    10201 tttcatgaca tcgtcctctt aagaagtgat aaacaagata gttgattgat caatttgata
    10261 catattacat tttcattata tcaataaaag aaagcgttgt caattatttt atttaaaata
    10321 ttgtcatatt ttaaataaaa tggtaaaaga gactcggtag cctcgtctct ctttatctta
    10381 ctgacctaac cttcttgcca attttcttgg tcttctttaa agcgttttag aagatcaagg
    10441 ccctctggcg tgatgtaacc ttcttcttgg gctagatgga taagctctct atagtttgaa
    10501 agcgtcacaa gtttaacacc agcatctgca aagttcttat ctgcttttgg caattggtag
    10561 ctgaaaatcg ctacaactcc aagtacatct gctccttctc gcttggctgc tgctacagct
    10621 tcaagaactg aaccacccgt tgaaataagg tcttcaacca ctaccatttt ttgaccttga
    10681 gctacgcgac cttcgatttg attaccagct ccgtggtctt ttggtttgct acggatgtag
    10741 gcaaaaggca agtccatctt atcagcaata atggctccgt gtggaatccc tgctgttgca
    10801 gttcctgcaa tcacttctac ttcaggaaag gcttctttaa tagcttccac aaaaccattt
    10861 tcaattaggg tacgagtttc tggataggct agtgtcacac gattatcagt gtaaatcggt
    10921 gacttgatac cagatgccca agtgaagggt tcctctggtt tgaggtaaac ggcttggatt
    10981 ttcaagaggt ggctagcgat atctttagca agtgtcatgg tattctcctt ttatttttct
    11041 aatctagttc tttaattcca gttctgtgtc cattcatcct tgatggcatg ataagctgca
    11101 acaggatcct cagcttgggt aatgggacgt cccactacga tatagtcact gccgatttga
    11161 taggcatcag caggtgtcat gactcgtttt tgatctccaa ctgcaacacc agctggacga
    11221 atccctggtg tcagacagat aaaatctgga ttggtagcct gcttgatgac ttgtacttcc
    11281 tgagccgagc aaacaacacc atccaagcca gcttcagctg tcttcttggc atagtgaatc
    11341 acagactctt gcagactggt ttggatattt tgaaactcct gcatctgagc ttctgacgtt
    11401 gatgtgagct gagttacagc gatcaatttg gcttgactcc caagaccttc acgcgccgcc
    11461 ttcatcatct ctacaccacc agccgcatgg acattagtca tatcgacacc aagctgagac
    11521 aagaccttca tggctgactt gactgtatta ggaatgtcat gaagtttgag atccaaaaag
    11581 acactatgac ccaaaccttt taagtaggac acaatctcag gccccgctgc gtaataaagc
    11641 tccatcccta ccttgagata aaggctttct tctgctggga aaagagctaa aaattccttg
    11701 accgcctcaa aactaggaaa atcaagagca atgattggac gatgttctcg cataggtttc
    11761 tccttttacc tttactagtg agagagtctg gccaacagaa gccacgaaaa aaggaacctg
    11821 ccaacagcaa ggttccacga aaaatgggta gtctccaccc tttcaccgtc taaccttagc
    11881 tgcctctctg gactgcttta aaggttttct attattctat tatttctact agcacttgtc
    11941 aagtaaaaac ttagtcacgt atgtgttgac tgctattgaa cacctcacaa gactctttcc
    12001 aaaagaatag ttaaagactt tcacttagga cagagaaaaa gagtttgctt gttacccttt
    12061 aattgaacaa atgggaattc tgttttattt tgcggatgcc tattcatctt ggcaaagggg
    12121 aagtaatgag aatgcgaatg gacttcttcg tgagtatttt cttaaaccga tttggcaaca
    12181 attaccgaaa aaatccttaa tggtgctttg ctagcttatt aatcaccgtc cccaaaaata
    12241 cttggattac aagacaggtt ttgaagcact tatagaggaa atttaaaaat gttgcatttt
    12301 ttattgcaat ttatcagtta tcttatcacc caaaacttct gcaattagta tgagcactat
    12361 tgttattttt cttttttcgt acactcaaat caaaatatag caataagact attctgtaga
    12421 attgctgtca aaaagatcag ttcatttttc cttcccactt gtgtttataa tcattaaaat
    12481 aactcaatta agaaattttc tttttcctaa tagttatcgt aattaaagtt aatagaagaa
    12541 caatccaaat aagtagtgtt agattaggaa ttagagtttt attccatgaa aatccctccc
    12601 attttgtgaa aaacttagta aacagtccca taaaactata atctgttatc ttcgcaactg
    12661 tcttatccaa accagatagc atcggtaaga aagcaactaa aatcatagca ggaagactga
    12721 taacctgagc tactacttgg ctcttcgcgg ttaaacctat caataaatag aataaaatga
    12781 tggataaaga ggttagaaga acgattgtaa tataattaaa agtatgtaca attgtaactc
    12841 ctaaaataag aggagtagtt cccataatca caaaagttag caaaaaagga agaaacatcg
    12901 ttgataaaat gtattcggat cctttaacac cactcaacag aagagtttgt aaattgtact
    12961 tttctttttc ttcagacaag ataatagtta taggacttcc aacagccaaa gccaaagaaa
    13021 aaggtaaaca catcatcaaa agaactaatg cctgttgatc gttgaccttc ccctgtgttt
    13081 ccataagata tttataaaaa tatgtgaaag caaaaggcac taaaacttgc aataaaatac
    13141 tcttattgga aaggataatt tgacaacgca accataccaa agccagaaac cgtttaagca
    13201 tttagctttc ctcctgttaa tgtgataaaa atatcttcta aagtaggctc acatgaatga
    13261 attgaaaaaa tattttccag atcagcctga gatacctgtt ctagtgatgt aaaatcaaaa
    13321 gttatctgat tcccattata atctgtaacc ttaatctttt tatctttatt atatctttga
    13381 atgagttcag aaggagctcc ttgctcaact aatttccctt tatttaataa ggcaacataa
    13441 tcacataaaa gagttgcttc attcatatca tgagtcgtta gaaaaatcgt tgtccctgct
    13501 gttttcaatt ctaaaattaa ctcatgaatt gttcgagaag ttgtgggatc tagacctgag
    13561 gtcggttcat ccagaaagag tacagcgggt ttgttgataa gagctcgtgc taaaagcatt
    13621 cgttgcctca ttccagtgga taattttcct gctaccatct tgcgactatc atataatcct
    13681 actcgcttta acaaattatc aacacgtgat ttactaatat tataaaattt actataaaaa
    13741 agaagattgt tatacagaga cattttctca taaaatccac ttgtatcgct aaccaatcca
    13801 attctcttta attcaccgct tgttaaattt tgagattttt gtcccaaaat aatagattgt
    13861 cctttatcgg caaggaactg cccagtcaga atattaatcg ttgtggtctt tccagaacca
    13921 gatggtccta aaaatctaac cttctttaac agtgaagctt atattattta aagctatttt
    13981 atcaccaaaa ctttttgata aatggctaac ttcaatcata ctcattgcac ctcctttata
    14041 ttctacattt ttacttaaca tctatttact tatctttatc aaataaacgt cttaaaaaaa
    14101 acaataacat taagcatata accgttagca agattgtttc aatattattg ataatacgat
    14161 acattctcaa gcagtataaa ataattatat atatgcaaat gctcattata cctatttttt
    14221 taaacatact aagcctcctt gctaaataat aaagttatat aataactaag acacactata
    14281 cctaacataa taaatatcca ctggtaactg aataaatgtt ctaaaattaa caagcaaaaa
    14341 taaaatctca aacgatgaaa aaagccatac tatattttcc aatgatgttt gaatattttt
    14401 attgtagtaa gcataatata tagcaaaaat aatacccaag aacaatacta ttaattgtgt
    14461 ttctataaca gtgaactgcc caatagcaga taaaaaagct actcctcctg ataataagca
    14521 aaaacaaact gaacgaataa atgttttcgc tctaatattg ttatctttca ctttattatt
    14581 tttttacaaa ttggacattc tccgttttcc agaacatatt tttcttgact tagagcatga
    14641 tttctcatat tataaacgta agcgttatct attaggattt cattataaca tcccagaaat
    14701 attttaatta ttaaattagt cattatccct cccaaaatag caggcatctc tggtgtagtc
    14761 ggccactttg tcttattaga taaagtgtac ttatttatat ctttgtttat attccgacaa
    14821 ccaaagcaag aaccgatggt agggatatat attatcagta ataagatagc atccattaaa
    14881 ccctcctgat atgtaggata tcttatgcga tacagcaaat tgattgacaa tttttataat
    14941 atcaatgggc gtatcaattg ctttaacgat aaaatctata ctcccatatt ccgcaacaat
    15001 tttttctaat tcttctactg aagaaacttt aatagaaata ggtactacct gaacatctga
    15061 attatacttg tgtatttttt cagaaagaac attaatcttc tccttaccaa catccgactc
    15121 tgtataaaga atttgcctat ttaaatttga tggttctatt atatcgtaat cagcgacaat
    15181 aagtttcctc actccataca tacttagact tagagatata taacatccta aggctcctgc
    15241 tcctaatact aatactgttt tattctgtat tttattatac tcttcattat aaaataacga
    15301 atagtaacct ttagttctag aatatctact atcaatatct atctcatccg aaattaaaat
    15361 ttgttcattt aatagttcaa atgttagttt actgcctaat acttcttcta tctctaatag
    15421 ttccttacaa ctattaaata gatcgttgat aaattcttgc gaaatttgag gttctatagc
    15481 tatttcccgc atactccgac cacgcaaata cattatacct tcttttttat aagcaaaaac
    15541 ataactattt acgtagaaca tatttcgtcc ttcctctcat atataatata gaaagcagta
    15601 tagaaacaac cacccctcct atactacgaa caataatatt aggtgtcaaa aactgtaata
    15661 gttgactcaa agggaaataa ataataacaa atgctattaa aagttgaact atatattgaa
    15721 aattcaagtt attattgtta gaattcaagt atcttaaaat aataccacta tcatactgaa
    15781 aaaaaggaat tgaattaata aaaatcatac ctaagttaat gataaaaaat aagagtccaa
    15841 gcaatttata cgatgtgaac aataaaacaa gtaaacctgt aagtgataaa atcgaatttg
    15901 aagcaattcc tgctaacata attttcaaaa attgtttttt attttttaag aattgcaccc
    15961 cacttaaatc tacaaaaaaa agccaaatta aacaaaagta gcattattcc acaatttggg
    16021 atctcaattt gataattatt agcaattatt gcatgactaa attcatgtat cgttgcagac
    16081 ataataacaa caagcaacca aaataaaaga atgtttagcg ttattggaaa gttttggaaa
    16141 ttagtcaaaa tatccgccct ataaatatac ataataaaac attgcaaaat aagagccaat
    16201 gggaaaatac caaataggat tttttcaaaa ttcttattaa atcttttata gtttaatttt
    16261 tcagataatg ttatattaaa gtcccaagat attttaaaaa tatttatctt ttttcgtgat
    16321 ttcatagaat ctataagtcc cattttacta agaacaatat aaaatcttaa ataatatttc
    16381 ttccctacta acactaataa gtccgatatg gtaaattctt gacctattag ttgctctaac
    16441 catttttttc ctgtttccca actaatacat gtttcttact ggatggtata tatagatgat
    16501 actgtccctc ataaggagta ggtaatatct taatcttatc aacaattctc ataacaaacc
    16561 taacctttaa taaataaaag aggggctaag taaaacatac tctagtccct cagtccgcaa
    16621 tcaataaaat aatgactaat tctattaatc tcttgctgca agactaatac ggaaaattca
    16681 aatgactgtc taaaatgaaa ttgaaaaagc ttgatgttag taaatgaaca aacttcaaca
    16741 accagcaaga gcaaggattg ctaaaccaaa tccgatacct gttgcaagtc cagctgaaaa
    16801 atcactaact ggtactgaaa tttcttcaat ttcctcaaat aaaaagttat cttgcatgat
    16861 tatcactccc tatcttttta acaacaggct attccaatta agagaactac cccagttcca
    16921 agtaaaactc ccccaaaaat tcaccatctg taggggctac gatttcttca atttcttcaa
    16981 ataagaatgc ttcatctgac atatgtgtta ccttcttcat aaaaaattat tttgtaatcg
    17041 attacattgc aaatgtaaca taaagaaaaa cagatgtcaa atattaaacg taagaacatg
    17101 gtcactaaag aactataaga gaaaaggtaa acctagcgac gcgatgagcg ctgggtcgtt
    17161 tgatttcgat tgctctcttc ctcttgtttt ttctgttctt cttcctgttt tttctcggct
    17221 tccttggcct cttgtttggc tttttcctca gcctccataa ttaatttatc cgccacagtg
    17281 taactgtaaa ttccagcttc catgtcgacc acactcggtt ctgacaattg tggcttaatc
    17341 ttactgtaat atggcagttt cttactcatt tcagatagag gaaccaagac ttcgtccgaa
    17401 tcattcatgg tcaatcgaat taaatcggat gtcaccttgc ttggggctaa ttccaccttt
    17461 tggatagccg ccttgagttc tgggctaatt tgagcaagtt ctgagacaaa aaccttgatt
    17521 tgttcactat cattaaagag aactgataaa taagtttctg gtaaactgtt cagactcaca
    17581 gaactagtct caagctgacc actggaaaga ataggataat gattttcacc agaaatatag
    17641 taggccacaa tatcatattc cttgacctta atagtgaact tagttggaaa ttgatagaca
    17701 agttgagctg attcaaccca atagttagac ttaatctgct tttcatattt tgccttgtct
    17761 agcagaaggt taatcgtata atccgaatcc tgaatgcctg aagcctgtcg aatatcatca
    17821 gctgtagttt gcaccgttcc ctcaacacga atatctttca tggtcgcata aggactgagc
    17881 aagtaggcag agacaatcaa taaaagcaga cttggaaata aaatcgtgaa ggctcgcaag
    17941 atatggatac caggaatctt tgctttggct ggtttttcct ttgtagcctt tttagaaagc
    18001 tttttatcct gttcctcctt ctctttagac tctggttctt ctttctcttc tttctctttg
    18061 tcagcctctg aggatgctac tttttcttca gactcttcct tagctgattc tgaatcttcc
    18121 tggtctgttt cactctcctg gtcctgtttg tcctctgact tctcagattc ttctcccatt
    18181 cgagcttgtc tttccttttc cttctcctca gctagagccg cctcttcttc agccttcttt
    18241 tttagatatt cttggtttcg tttctgccat tctgataact ctttcaattc ttcgaggatt
    18301 tctttgtcct catttttctt atcttttgac atttactttc cttatgataa atcttttttc
    18361 aacaattgat aaaaatctgc tagagatttc aattccttag aagccttcat cttagcttgg
    18421 taatcttcct tgtgacttag taagtgagaa agcttctctt ccaaactatc caaggtcaaa
    18481 tcgctttctt gaaggtcttc tgcatagcct ttttttacaa agtaagctgc attttcaagc
    18541 tggtcaccac gactagcttc acgaccaagc ggcacaatga catgcaattt tgctatcgcc
    18601 aagagctcaa aaatcgtatt ggcaccacct cgtgtcacaa caatatcagc caattccatc
    18661 aagggttgat agagatcggt cacatagtca acacgaaaaa gattttggct caactcattc
    18721 aggctagaat ctccagttag attgataata ttgtagcgct ctgttagttc tttcttatgg
    18781 tctgtcacca attggttaaa gacacgagca cctgcagaac cgccaacaaa caatacagtc
    18841 ggcaatttat gattaaagtg gctttgaata tccaccaatt catctggttc tggatttttt
    18901 tgatctgaaa ccttggccac tgctcccaca tgctcaacct tagacaaact cgaagcttgc
    18961 tcaaaggttg aatacatctt agtcgcaaat ttataggcga ttttattggc caagcccata
    19021 gacaggtcag attcgtgaat aaagacaggc actcctgaca cacgcgcagc gataacaggc
    19081 ggtactgaga caaagccccc ctttgaaaaa agggtctgtg gacgaagtcg caacatgata
    19141 aagagcgatt ggacgattcc ccagcaaact ttgaagacgt ccagcatatt ttgccaagag
    19201 aaatagcgac gcaattttcc agtcgcaata gaatggaagg tgacatccaa acctgactta
    19261 aggatttctt ggtgttcgat accacgcttg tccccgatat agtggacttc ccaaccatct
    19321 tcgatgaact tgggcattaa caaaagattg agggtaacgt gtccaaccgt ccccccacct
    19381 gtaaagacaa tttttttcat attattcttt taactccgct actgtgtcga taaagaggtc
    19441 gccacgtact tcaaagttag catacatatc ccagctggca ttggcaggac taagaagaac
    19501 cacatctcct tgagtcgcaa gctcataggc cttgcgggtc gcatctgcaa tatctgtcgc
    19561 ctccacataa gcgacaccag ccttgtctgc tgcccgtttg acacgttctg cagattgacc
    19621 caggatgacc atcttcttga gtccagtaat gtctggcacc aattcgtcaa actcattgcc
    19681 acggtccaaa ccacctgcaa tcaagacgac cttgctgtta tcaaatcctg acaaggcttt
    19741 ttgagtagcc aagatattag ttgatttact gtcgttatag aatttaacac ccttgatgtc
    19801 atccacaaac tggagacggt gtttgacacc accgaaggct gaaagagttt ccttgatggt
    19861 ttgattgtcc acaccacgaa gcttggctac agcaatagtc gcaagggcat tttccacatt
    19921 gtggctacct ggaacaccga tttcattcgc tgccatgact acttcaccac ggaagtagag
    19981 ttgaccatct tccagataag ctccatcaac cttttcaagt gttgaaaatg gtacaacagt
    20041 ggcttctgtc ttggaagtca agtcttttgc caagtcttga ttaaagttca agacaaggaa
    20101 atcagctgct gtcatcttgt tctggatatt ccacttggct gctacatatt ccgaaaatga
    20161 cccatggtag tcgatatgag ttggcatgag gttggtaata accgcaatct ctggatggaa
    20221 ttcttgaaca cccatgagtt ggaaagaaga aagttccata acaagcgtgt ccttatctga
    20281 tgctatttga gcaacctgac tagctggata gccgatattc cctgataaaa gaccatgttg
    20341 cccagcagca gtcaaaactt ccccaatcat agtcgttgtg gttgtcttac cgttcgatcc
    20401 tgtgatacca ataatcggtg cttctgaaat caaataagcc aattccacct cagtcaagac
    20461 tggaattccc ttggccaaag ccttttcaat catgggattg ttgtagggga tacctggatt
    20521 tttcaccata agggcaaact cttcatccaa gagttccaaa ggatggccac ctgtaatgac
    20581 cttgatccct tcttccagca aactttgggc agctggattg tcctcgaaag gtttcccatc
    20641 atttactgtc acaatggcac ctagcttgtc caacaaacga gctgcagatt caccagactt
    20701 ggccaaacct aaaacaagga ctttcttatt tttaaattga tctattactt tcatgtctcg
    20761 aactccattt ctactcctac tattttacca tttttatgga aataaaaaag ccacaaagtg
    20821 tgtttgtgac tctttcttct aactgaatct taccatatca tctatgtgat aaatcggtaa
    20881 ctcgaatgac ctgatccact tgttcccaaa taagaggatt gtgggtcgca ataataatgg
    20941 tccgattcgg attttttaaa gattctagga tggaaagtaa ttcctcagag tttttggggt
    21001 ctaaggaagt ggttggttca tctgcgagga tcaaaggtgg atcctttaaa attatcttcg
    21061 ctaatgcaac acgttgtgct tctcctcctg ataactcaaa tataggttgc ttcaaatcca
    21121 aataagagag gtttacacag tttagagctt gtttcatcaa agagattttc tctttttcct
    21181 tcaacttttt accaactaaa cccagattga gattctcttt gacggtttgg ctttcaatta
    21241 agccaaaatc ttgaaataag tatcccaagt aatctctaaa gaaaacagaa ggcttgatgt
    21301 ccttaagaga agtaccatca tagatgattt gtcctttgtc atatggctct aatcgtccaa
    21361 tcatattcaa gaatgttgtc ttaccacagc cacttgtacc gattaaggca taaattttcc
    21421 caccttcaaa atgaagattt gtatctgaaa atagctgacg gcttccaaat tttttagata
    21481 tattctttag ttcaatcatc ctattttcct ttcataattg tcatagaaac acgagattct
    21541 ttctgcgctt gacggtaaag cgtcagaact gcactagcta gaaagactaa taaagtgagc
    21601 aagccaatca ccaagtctcg actgcttaaa ataaagagac tagcaccaaa tacaaaactg
    21661 gcaaattgac taaccatata ctgagcatgt gtttcaaaaa atcgtaaacc tgaaattcgt
    21721 ttaatcaaaa tatctcggcg gaattgctcg aaatatagaa gattgacaga ataaaatagt
    21781 aataaggaac tagctatccc cacaatagct cctaagatta aagttgctgt ttcagtttga
    21841 acttcattat aacgagttag ataaacactt cttccttctt taagatagga tacttgctca
    21901 taaattccag ctttcttcaa gagttctagc ccactctcat atcctttgat aaagagttgt
    21961 tttccagcat tgatagacca actagataag gatataaaac tatcacctgt agaagtcggc
    22021 gtgaatacca ctaaaatcgg atcagtcaaa tactgagtag atacgggatt ctcaccgtta
    22081 ttataaacaa accgcttttc tcccattgaa agataactaa cgtgcgcttt catctcataa
    22141 tccaaaggag cacttgcctc ctcaccagat tttccataat aactcaatct ttcttcaaaa
    22201 actttcttaa gttctgcttc tcgagagcgc aaatgttctg ggagcaagag gataaactca
    22261 cctttttgga gatgggctaa cttctgtttg gtctcagcat ctaccacgac cttttccttg
    22321 tccaaataac tgggactaac atagagcgta ttagcatctg gactataggt atccagtgtc
    22381 tctccctgtt cattttttcc ttgtggattg gcaaaatgga gcagattatc ctttacataa
    22441 agagcttgtt cttcttcgat tgcttccttg gcaaaggcat accacttgct ctgattttct
    22501 gtatcttttc ctctatcacc taagccaaaa gaaatttgat aatagtctgc tctatcctgc
    22561 catgcttgtt ttgaaatttc aagttctttc aatcgttggt aagacatcaa acctgtctta
    22621 acagcgtagc ctactgtaaa gacagcaacc aactgacaca atagggttaa aatcatcagg
    22681 cgtttaaggg gtaatctccc cttaataacg ggaactaatg ctttgtaact caaactcatt
    22741 agataaagaa gcattagtaa aattgaaatc gccaataaaa acaacagata gaaactaatc
    22801 ccaaaaccat aggtggctaa caagatagga taaaacaaac cttgactaaa aagaacgact
    22861 cccccaccta ggaaggaaag gagggctgat agaaggagcc atttgatatc agtagataaa
    22921 gaatgcccca tgatggataa gagagtctga ccagaaaaga gttttatacc tgctgctctc
    22981 atttccttaa tccgagtgat aatcactaaa gcaaagaaag ataagccaaa tattgctaaa
    23041 ctaattaaaa taaggggatt tagtaatatt cgaaaagcaa gaaaataggg cggtatcttt
    23101 cggtcagcac ttgctttata acccaaatct cctaatttat cggcaagctt ttctttcgtc
    23161 aaggagcctg acaaaaggag ataactattt agcggatcac tacgttcacg actttcttgg
    23221 ctagcttctt ggaattcttt tggtaaagtt ccctgaccat aagttgcata agtaaagtga
    23281 gtcgtcccat ccttactcgg ctctacaatt cttctagcta ttaaactctg ttctgagttt
    23341 gcaaaattct ccaattcctg ttcaaatacc tcacgcgtcg gttcctgagt atcttttttg
    23401 acacgaagta aagaaacgga atcatagctt gcatataaat attgtggcgc acgtaagaca
    23461 ataatccaag caaggaagaa gctgagaaaa aaagttgata ataatatgaa tagtttcttc
    23521 atagtagact ccttgtaaac aaaattcccc ctgtaatttc ttacaagggg aacgatttaa
    23581 atcaatgaac gattagtcat aatcacagta aaatgctact tgttctcccc atttagtcca
    23641 aatccatgca taggaggttt ttccagcaga agcttctcct ttatctgaat gactattcca
    23701 tctacttaca agagaagaat aatgatattt tttaccatga tagtaatttg aaaaagccca
    23761 accacctcct gaaccttctc catacgtcca tactcctcca tctggatatt gtacagcagc
    23821 tgatgcagct cccaataatg taaaacttga aataagagct agagcaagta atctatgttt
    23881 tttcgttttc attttatttt ttctttcaaa aaaagcacac cttgagcaac aatgcaacaa
    23941 aataaaccct cctctctctt ttgttgaaac cgctttctta tgtgataaaa atatcatttt
    24001 tattatttgt tgtcaaggaa aaaatcgaat tttttagata ttttactaca ttgcctctat
    24061 gaataatatt atatagtagt tttattttaa aataatatgc aaccagtact aaccaaatat
    24121 aaaatagatg ccattaacga attttattca agtttttccc attcatacta tccaagcaaa
    24181 agagatgatg ttatagtaca aaacaattca aactattgta aaattcctag caaaaaagag
    24241 agccaaaact ctctttttta tcttctttta ctttttttga ctggcatgag tgtaatgtct
    24301 ctaacactaa agtaagctag gatcaacatg gctattgcta ggaatatttc tgttggtaat
    24361 tgaaaaattt tcagaaaaga tagaaccaat aaaatcaaga gtgccactaa aatacatacc
    24421 atagcgacga tattgactgt ccctttaatg ctttctggtg tcgcaaatac atagagtagg
    24481 agcagtaaaa ttcctaggac taaatagacc atctttctct ctttctagct cttattcagc
    24541 tgattttttc ttcttgttag ctttctcacg ctctgctttg ttaaggattt gtttacgcaa
    24601 acggatagac tcaggcgtta cttccatgta ctcatcgtcg ttcaagaact caagagactc
    24661 ttcaagtgtc aagatacgag gcgtcttgat aacagctgtt tggtccttag tagctgaacg
    24721 aacgttggtc atttgttttg ccttcgtgat gttaactgtc aagtcatttt cacgagagtt
    24781 ttcaccgatg atcattcctt cataaacctc agtacctggg ttgacaaaga tcgtaccacg
    24841 ttcttcgata gacatgattg agtaagttgt agccttacca gcatcgatag aaacaagggc
    24901 accacggtga cgtccaccaa tttcccctgg aatcaatggc aagtattggt cgaaggtatg
    24961 gttcatgata ccgtaaccac gagtcattga caagaactca gttgagtatc caatcaaacc
    25021 acgcgctgga acaaggaaga ccaaacgagt ttgaccatta ccagttgaaa tcatatccaa
    25081 catttcacct ttacgttcag aaaggctttg gataacagac ccttggtatt cttctggagt
    25141 gtcgatttgt acacgttcaa atggttcaca tttaacaccg tcgatttctt ttacgataac
    25201 ttctggacga gatacttgaa gttcatagcc ctcacgacgc attgtttcga taaggattga
    25261 caagtgcaat tctccacgtc ctgaaacagt ccatttatct ggtgaatcag ttgggtcaac
    25321 acgaagggaa acgtctgttt gcaattctgc ctgcaagcgt tcttccacct tacgagaagt
    25381 tacccattta ccttctttac cagcaaatgg tgagttgttg accaagaaag tcatttgaag
    25441 agttggctca tcgatgtgta ggattggaag agcttctact gcatctgtcg gagtgatggt
    25501 ttcaccgaca aagatgtctt ccatacctga aacggcaatc aagtcacccg ctttggcttc
    25561 ttggatttca cgacgttcca aaccaaagaa accgaagagt tttgtaacac ggaagttttt
    25621 agttgtaccg tcaagtttag aaagggtaac ttggtcccca accttaactg taccacggaa
    25681 gacacgaccg ataccgatac gtccaacgaa gtcattgtag tccaaaagtg acacttggaa
    25741 ctgcaaaggc tcatctgagt tatctactgg agctgggata tggtcgataa tcgtgtcaaa
    25801 gattggtgcc atagtcgctt cttggtcagc tggatcatct gacaatgaag aagttccgtt
    25861 gatcgctgaa gcataaacca ctgggaaatc aagctggtcg tcatctgcac caagctcgat
    25921 gaaaagttcc aagacttcat ccactacttc tgctggacga gctgatggct tatcgatttt
    25981 gttaacaacc acgattggga caaggtcttg ttccaaggct tttttcaata cgaaacgagt
    26041 ttgtggcatg gttccttcat aggcatctac gaccaagaca acaccgtcaa ccattttcat
    26101 gatacgctca acttctccac caaagtccgc gtgtcctggt gtgtccataa tgttgatacg
    26161 agttccgttg taagcaacgg cagtattttt agcaaggatg gtaatcccac gctctttttc
    26221 gatatcgttt gagtccatag cacgctctgc caattcagta cgtgcatcaa gcgtttctga
    26281 ttgtttcaat aattcgtcaa ccagggttgt tttaccgtgg tcaacgtggg cgataatcgc
    26341 aatgttacgg atatcttctc ttaattttgt catgatttcc tctataatat tcaaaattta
    26401 ttttctaact gaacgattat accataattt caaataaata acataactca agcaagtgta
    26461 aatgttttca ctccgctttt cttttcacgt caagcctttt caaagcaagc gacttatgat
    26521 aagataggca cagtatgcgt ttagataatt tattagccca agaaaaagtt agccgaaagg
    26581 ccatgaagca agcactcctc agaggggaaa ttctagtcga tggttgccca gcccgatccc
    26641 tagctcaaaa tatcgataca ggactacaag aactcctttt tcaggatcga atcattcaag
    26701 gctatgaaca cacctatctt atgcttcata agcctgctgg tgtcgttaca gccaacaaag
    26761 acaaggaact tccgaccgtc atggacctgc ttccatctga catccagtct gacaagctct
    26821 atgccgttgg ccgactggac cgagatacaa cgggactcct cctcttgacc gataacggtc
    26881 ccttgggctt tcagctcctc catccccaat atcatgtcga taagacttac caagtccagg
    26941 ttaatgaact tctaacacct gaccatatcc aagcctttca aaagggaatt gtctttttag
    27001 atggtactgt ctgtaaaccc gcaaaactag agattctatc tgcaagtccc tccctcagtc
    27061 aagcctctat caccatttca gaaggaaaat ttcatcaaat caagaaaatg ttcctctcgg
    27121 ttggtgttaa ggtgactagc ctcaaaagaa tccaatttgg ggacttcaca ttgaacccag
    27181 atttagcaga aagtaactac cgccctttga accaaaaaga gttacaaatc attaaaaact
    27241 atttagagat gagtcgataa aacaaaaaaa gctttaaaac taaagctttt ttcttttatt
    27301 taccgaaaaa ttaaggcgat tgctacaatc cagttaacta cagaaatcac aattcctaag
    27361 atattaagaa tcttttctat tttatagtct aattgtgact ctttttggta tgaaatagcc
    27421 aagaccaatc ctatgatacc caaaatcaag cctacaattg gaaataacaa accaagaata
    27481 atcgacaaga tacccacaaa aagtggattt ttcttctttt ctttcatgtt ctaagaactc
    27541 tttaaatttt atacaaatta attatactat aaaacaatag cttcatccta tcattcgact
    27601 aatttggaaa taaggttagc tagtcttcac tttccctttc caagaatcca agccataaga
    27661 aaggatataa atctcagaaa aaccttgttt tttcaagtaa agagctgcat ttgtaactcg
    27721 ttgcgcacgt tggttctcgt agagaaggac aggtttatct ttacgaaggg ctgcaagact
    27781 agttttcaac tgacttgaag gaatattgcg tgcaccaagg atatgttttc tgtggaattc
    27841 tgctgggtcg cgcaaatcaa tcaattgacc cgtacgaatc aaggcttcaa actcctcatt
    27901 gtccacaatt ttagccgcac gacgaatacg aagatagtta aagcccatcc acgccaacat
    27961 tgctagtata agtgcccaca aaatccaagt aaccattagt tcttttctcc atttttctca
    28021 atataatcca attctacctt gtgctctctg cgaagaactg cttctgcctc tagatagtct
    28081 aatttatcca tcaaccctgc atcgtaaatc cgagatagtt ccaacttcat cagttcaata
    28141 tcatataagc gttttcccat gtaaacaata ataccaaatc gtttgaggaa ttgctgcaca
    28201 tcatagaatg ttttcataag actcattcta gcaaaatttt gtgttttttt caagaagaga
    28261 ctcacacaat gctccttatt ttcctatctt ctttggcgat tctaaggcaa gtgtggtaca
    28321 ataaaaacat gagaattcaa caattacatt atattatcaa aatcgtcgaa actggctcta
    28381 tgaatgaggc agccaagcaa ctctttatca ctcagccaag tctttccaat gctgtgagag
    28441 atttggaaaa tgaaatgggc attgagatct ttatccgcaa tcccaaggga atcaccttga
    28501 cccgtgatgg gatggagttt ctctcttatg cccgtcaggt tgtcgagcaa actcagcttc
    28561 tggaggaacg ctataaaaat cctgtcgccc accgcgaact ctttagcgtt tcgtctcaac
    28621 actatgcctt tgtggtcaat gcctttgtct ctttgctcaa gaaaagcgat atggagaaat
    28681 acgagctttt ccttcgtgaa actcggactt gggagattat cgacgacgtc aagaacttcc
    28741 gcagtgaggt cggggtcctc ttcttaaaca gttacaaccg tgatgtttta accaagatgc
    28801 tggatgacaa tcacctacta gcccaccatc tcttcacagc gcaaccgcat atctttgtca
    28861 gcaagaccaa ccctctggca aagaaagaca aggtgaaact gtctgatttg gagaatttcc
    28921 cttacctcag ctatgaccaa gggacgcaca actccttcta cttttcagaa gagattcttt
    28981 ctcaagaaca ccacaagaaa tccattgtgg tcagtgaccg tgccaccctc tttaatctct
    29041 tgattggttt ggatggttat accattgcga cagggatttt gaacagcaac ctaaacggag
    29101 acaatatcgt ttctatccca ctggatattg atgacccgat tgagctggtc tatatccagc
    29161 atgagaaaac cagcctatct aagatgggcg aacgctttat cgactatcta ctagaagaag
    29221 ttcagtttga tagttgagaa atgataagaa ccaatatgta ggctagcaac aacctgcaca
    29281 ttggttcttt ttacttataa ttaaaagttt cccctgccaa cttatcagct agcttgggaa
    29341 agagagtata aaacttatgg gctaggttca acaaaatcgg gagattgagt tctcgtttat
    29401 tttttcctat aatcttgaca atctttttag ccactgcatc tgcctctaag aggaagcggt
    29461 caaccgattt aagataagtt ccatctgggt cagcttggtc aaaaaatcct gttcggattg
    29521 gacctggatt gactgttgtc acatagactc catagggcat aagttcgagt cgcagagcat
    29581 ttgaaaaacc aatagccgca aacttggtcg ctgagtaaag actagacttg ccagtagcta
    29641 ttagacctgc catgctgacg atgttgatga tatgcccttt gctgctttcc ttcatacgag
    29701 ccgcaaggtg acgagacaga ttcatcaggg caaaggtatt gacctcaaac atctggtgaa
    29761 tatctttatc agcaatctgg tcaaatccct caaaaatccc gtaaccagcg ttgttaatca
    29821 agacatcaat cttgccatag cggagataaa gatcagttac cagagcttct agggctgaat
    29881 cgtcggtaat atcaatttca atcaattctg catgggaata atttccgtag agttgggcta
    29941 atttttcctt atttctacca agcaagatga gttggtcatt gggcaggagt ttgaccattt
    30001 cttgagctag accaccgcta gctccggtaa tgagaatagt aggcatactt atcctttctg
    30061 tgactgctag atttccactt cttccaagtc tttgaccaca tggacatttt caaaaattgt
    30121 ggcagcgtcc ttcttgagtt tgcttatatc ttttgagagg aaacgggcac tgatatggtt
    30181 gagtaggagg cgtttggcac ctgcttctac cgctacttgt gcagcttgca tattagttga
    30241 gtgaccatgg ttacgagcaa ttttttcatc acccttgcca taagtggact catgaactag
    30301 gacatctgca ttgacagcca gacgcacact ggcatccgtt tttcgagtgt ctcctaaaat
    30361 agtgataatc ttacctggac gtggcgctga gatatagtct gctgccttga tttcagttcc
    30421 gtcttccaaa acaagatcct ggccgttttt gattttacca aaaagcgggc cgaacggaac
    30481 accagcagcc ttgagttttt cagcatccag cgtcccttct agatcctttt gcatgacacg
    30541 atagccaaca cagaaaatag tgtggtccag ctcctctgca tacacagtga atttatcggt
    30601 ttcaagaatt ttacccagag aatcttggtc aaactcatgg aaatgaatgc ggtagggcag
    30661 acgagaacct gacacacgaa ggctggttaa gacaaatgac ttgattcctt gaggtccgta
    30721 gatttccaaa tctgtctgct cttcattggc ctgaaaggca cggctagaaa ggaaacctgg
    30781 caaaccaaaa atgtggtctc catgcagatg agtaataaag attttgctga ccttacgtgg
    30841 tcgaattgtg gtttccagaa tgcgattttg cgtaccttct ccacagtcaa agagccaaac
    30901 ttcgttaatc tcgtccaaaa gtttcagggc gagacttgaa acgttgcggg ctttagaggg
    30961 ctgaccagcc cccgttccta aaaattgaat atccattcga tactttctaa ttaatcaata
    31021 tataacatgg ctgtgcggtt ttccgatcgg aaatagcgtt tgccagaaaa agcagcagct
    31081 tcttgcaata aatcctcttg gctgtagcct ttgagacgtt ttcgaccatc agccaatctt
    31141 tccaaatcag tcaaagctgt gagactttct aggctgacaa cttcctcgtc cttgacaggc
    31201 ttcatgtaaa tcttaccaga ctcttcaaag actaattgat gggagaaaat ttgcgcaatt
    31261 tcaaagagca agtcatccga gattttctcc tcattttcag agaaaatccg accaaagccg
    31321 tcactctcat aacaaaaacc aaaggattta ccagacagat taagccgaat aaaaggctta
    31381 ttttctaggg tgaaacttgg ctcagtattg taaagattca gttcctgact gagttctgca
    31441 aaataatccg tcgcagcctg aggacttttt ttctgataga gttctgcaaa gtaggcatta
    31501 acaacacttg gcggaggtgt aataagtgtt aactgctcct gctctgtttt accagctaga
    31561 agctgatcca gatagacctt gtccagactt gtataacctc catactttag agccaaagtt
    31621 ttaatatcag tcataaaatt cttctaacct ccatttattt ttctcggaaa tgtagcctgt
    31681 aatcacttcg ccgtcttcct gataatcacg ttcttccaga attgcaacac tctctaaatc
    31741 atgaatcttg taggactttg aaaaaggcac tcgcagggta aatgcttcaa aaatttcctt
    31801 aatcttatct agcaataatg cttgcaagtt ttcacgactg tcctcagact tggcagaaat
    31861 gagggtatat ggcgtttggg taggcgtgaa atcctccacc aaatccgctt tattataaag
    31921 cgtcaagtga ggaatatctt ccatgtccag gtctttcatg atggagagaa ccgttttttc
    31981 atgctcctcg tggtaaggat tgctagcatc gataacatga accagaaggt ccacatgctt
    32041 gctttcttcc aaggttgact tgaaactgga caccaactct gtcggcaaat cttggataaa
    32101 gccaacagta tctgtcaaag ttacttggag attgcctccc agatgaatac tcttggttgt
    32161 cgcatccaga gtcgcaaaga gctcatctgc ttcatactgg gtcttactgg tcaagatgtt
    32221 catgatagtt gatttcccag cattagtata accaatcaaa ccaatcttaa aagtgctaga
    32281 ctccaaacgt ttttctctga cagtcgcacg atttttctca accaccttaa gctggcgctc
    32341 gatatccgtg atttgattgc gaacgctacg acggttcagc tccagttggc tttcaccagg
    32401 accacgggaa ccaattcccc ctgcctgacg gctgagcata atcccctgac caaccaagcg
    32461 aggcaagagg tatttgagtt gggctaggtg gacttggagc ttcccttcat ggcttcgagc
    32521 ccgcatggca aagatatcca aaatcaactg catacggtca atgaccttaa caccgagaac
    32581 ttcctctaga ttgacattct gccttggggt cagacggttg ttgacgatga cagtagtgat
    32641 ttcttctgca tccaccataa gcgcaatctc ttccaactta ccagagccga cgaaggtctt
    32701 ggaatcatat ttttcacgtt tttgtctgta gctatctaca acgactgccc ctgccgtttt
    32761 cgctaaacta gccaattctt ccatggagag gtcaaaactg tccataccct gcaattccac
    32821 accaatcagc aggactcgct cctctttttt ctccgtttca atcatctaaa aactcctcta
    32881 tctggcttaa aatgcggtct tgtacaccag attctccaat ctgataaaag gtgacctgca
    32941 tgcgattacg gaaccaggtc agctgacgct tggcaaaacg acgggtcgcc tgtttaagac
    33001 tctcacgagc ttcctccaag gtctgctctc cacggaaata aggaaagagt tccttatagc
    33061 caattccttt agcagcctgt acattagggg aatggtcaaa cagccacttg gcctcatcca
    33121 aaagcccagc ctcaaacatc aaatccactc ggtggttgat acgctcataa agttgactac
    33181 gttcatcatc caagcagata atcagcggtt catacaaaat ctcttgattt tccaaatcct
    33241 gaccaaaatg ggcaatttct aaggcacgca tagcacgacg acgattaaac tggggaatct
    33301 caaggcctgc ttgctccacc aaatgggcta attcctcatc tgaatatggc tccaaactag
    33361 ctcgataagc taaaatctcc tcatgaggag tctccccacc taggtggtaa ccttctagca
    33421 agctctggat ataaagtcca gtcccaccgg cgataatggc tagcttgcca cggctgtgaa
    33481 tatcctcaat agtcatctta gcttctgaaa caaaatcaaa agccgagtaa gactcagtta
    33541 tctctctaac atcgattaaa tgatgaggaa cagctgcctg ctcttctgga ctagccttgg
    33601 ccgtcccaat atcaagtcct cgatagactt gctggctatc tccactaacc acttcgccat
    33661 taaagcactt tgcaacttca atagctagag ccgtctttcc aacagcagtc ggtccaacaa
    33721 tcacaattat ttttgttttc atcttttttc cttgaaaaat tcccattttt tcgttactat
    33781 tattataaca caaaaaggtc agtcagaaaa atgtgacctc ttgaggaggc tggctgattg
    33841 agaatataaa ggaggaagac tcatggctaa aggattcgct aaaggtcttg taacaggtgt
    33901 cgcaggaact gtcgctgccg ttgcaggtgc agtatacgca tttaaaaaga aagtaatcga
    33961 accagaagag caaaaagcag ctttcatcga agaaaaccgt aaaaaagcag ctcgtcgccg
    34021 cgtatcacgt taagaaacaa aagaagttgg gattattctc aacttctttt ttctattctt
    34081 ttaaatagct aggtcaaact tcaactgtgg cttaacaggg tcataatcca ccaactcaaa
    34141 atcttctgct ttgatatcaa agaaattagt cccatcagga acatttaaaa ccaagcgtgg
    34201 ttggcagttt gacggctccc gacggagcaa ttcctgagct tgttcaaatt gattatcata
    34261 gatatggagg ttgttgatga agtagaagaa cttcccaacc ttccagccaa aatgtttagc
    34321 aatcatcatc tgcaaagcca catactgcat agcgttgatg tggtgggcca ccagcatatc
    34381 attggagcgc tgggtcaagg tcgcatccag atagatttcc ccatcaacac gccgaacatc
    34441 aaacatggtc tgaaaggcgc acgggagcag cccatctgtt tcttcgaaag cttggtaatc
    34501 ccagagagaa ataatattgc ggcggttcca aggattggtt tccaactgtt tgagaagctt
    34561 attgataatg tcgtgtttct taacaacggc accatagcgc tcaccaatgg ttcccgtgtc
    34621 tccaacttcc cagtcattcc agtagtgaac attgtacttg tcattaagca cttctaagct
    34681 atttgactgg tcttggtaaa tccagagaac ttctttgatg gcggatttga ttgcaatggg
    34741 acgcaaggtt atgataggaa attctccttt agataagtcg tactcggaaa aggcaccagt
    34801 tacatacttg gaattagcaa cagtcccatc cttgtactta ggacgagcct gctctgagaa
    34861 aacaccttcc tttagaatgc gttcaatatt ctctttaaaa atcgtatctg cttttgtcat
    34921 ttactctcac ctcattcatt gtcctaacta gtatagcata aaaaataaaa gaggaacatt
    34981 actaactcta ggttagcatt ttcctctctc attatcttat tgcaatacaa gtgatgctgc
    35041 tccgataact ccagcgtcat ttcctagagt tgcaagagct aatttagtag atgtgcgtac
    35101 ttgtgggaaa gtattttcgt catagacttt ttgaacacct tgtagaagga attctcccgc
    35161 agctgacaca ccaccaccga tgacgattgt tgatgggttt aggattgaac caatgttagc
    35221 acaagcgatt cccaagtaac gtgagaagtt acggtaaaca atcaaagcaa ggtcgtctcc
    35281 ttcttttgcg agatcaaaga cagtcttagc agttacttct tctccgttat caatcaagcg
    35341 tttcaaggct gcatcgcctt cgtattcatc ggcatagcga cgagtcaagt tgacaattcc
    35401 tgttgcagaa gcgactgtct caagacagcc tttcttaccg caagtacatg agattggctg
    35461 gtcaaagtca acagtgatgt gacccaactc acctgctgca ccagcaacac cgtgaagcaa
    35521 tttgccttct gcgacgatac cgccaccaac accagtaccg agtgtcataa agacaacatc
    35581 tggttggtta tcaccagcac ccatccagcg ctcaccaaga gctgctacgt tggcatcatt
    35641 atcgatgaaa aatggaatgc ccaaagcttt ttcaatcttt tgtttgattg gttgaagggt
    35701 tttccagttc aagttgtagg caccgataac agtccctttg tcacggtcaa ccacacctgg
    35761 tgatcccatt ccaatgcctt ggaagtccgc tgctgccaat ccaagcaagt ccaaacgatg
    35821 ttgaatagac tcaatcatat catcaacgat atgacttccc tcatccaaaa tgttggtctt
    35881 gattgaccat tttccttgga tttctcctgc tgttgttaag attgcaaatt tgatagaagt
    35941 tccaccaagg tcaatcccaa taatcttttg actcattata ttctcctttt tcattgtgta
    36001 gtaattgaaa atgcttacag tactagtata tcaaaatcca ttcacttatg caaaggtttg
    36061 cgtaaatgaa ttataaggaa ttttttcgcc taccagtatc tgttaaccat ctaaatgatc
    36121 aatggcatat tgagctgcat catttgaaaa tttatcaaaa tcagaagtta attgtctata
    36181 catacgtttt tttgacatgt ggaataacga attataactt tttgcctttc ctaaagcagc
    36241 agaatattca ctattgctat cattactaga agaagctgta cttgcttgac cttccttcca
    36301 aactccgtca gcgccaacat aataacgtcc accatcaacc cattcattga cagccatttt
    36361 accatccgat tttagatagt aatttccttg ccatgtacta taagcataag aaccatctga
    36421 tgtcaaataa taccaagctt gataggtggc atcataaacc cattcacctt tagccatttt
    36481 accatctgat ttcaaatagt agtttccttg ccatgcattg cgagcatatg aaccatctga
    36541 tttcaagtaa taccatgctt ggtaagaaga atcataaacc cactcacctt gtgccatttt
    36601 accgtttgat ttaaggtaat aagctccttg ccatgcattt tttgcataag aaccatctga
    36661 tttcaaataa taccaagctt gataagaaga gtcataaatc cattcactct gtgccatttt
    36721 gccatcagct ttcaggtaat agtttccctg ccatgcattt ttcactagat ttccatttcc
    36781 atcaaaatag taccaagaac cgtcctgctt aacccaacca cttgctgtgg atgaagttgt
    36841 atcagtttga gttgtagccg cttgttttgc tttaaaagta cccttaggag catttttcaa
    36901 ttcgcaagcc acaccatcat gatcgcggtc taacttggca gaataaccag gttctccctc
    36961 atgaagatcc gaatatccat tagcccaagc ttccttacag ctcgaaaaat ggatgttttc
    37021 ttccgccaaa actggttgtg aaaacaaggc taaaattggc aacgtcacca gactcatttt
    37081 taaaaataga cgtttattca tttctttctc ccataattat gatatcgatt tcaatattat
    37141 tttatcaaac ttatttcagc aattcaagga aacttctggt cttttctaca aactcctttg
    37201 gttttgcctt attcaagaca tgagggccat caggaatgat ctgaaactgc gaatctgtta
    37261 acagtctatg gagagctttc atagaactaa gattcggttt atctttgctg ccacaaatta
    37321 gtaaggttgg ataggagcaa gtccctgcta tatccgttaa atcaagtgtc ttcaattcct
    37381 cagaaactcc gaccataaga gtcttgtctg ctccctgttt ttcaaatact cttttgggaa
    37441 gtagtttaaa aatcagcaac tgaagataaa ataggatatt ccctgctaat ttaagcgggc
    37501 atcctgacag aatcaaagct cgaagatttg gtaaatcgta actggaaagt tctagtgtca
    37561 gggcagcacc taaggacaat ccaatcaaaa caaaaggttc tgtctcttga gctaggtgct
    37621 gataaactcg ctctttagct tgttgatagt tactaactcc agaaggaaat aactcgatag
    37681 cctcagaagg ataatctgtc agtagattcc gaacttcttt ccaagactct gctgactgcc
    37741 ctaacccatg caaaaatatc aatttcatct agttctcctc aaggcttagt tcatacaagc
    37801 ctctcactgc attacagccg taaatagctt ctgcttgggt taaatctgcc aaggtcaaga
    37861 ctttctcttc tacctgtcct gtttctagca aatactgacg gtaaattcct ggtaagattc
    37921 caagtcggat aggcggtgtg tagagttttc cagcgatttt cagaaccaaa tttcctatag
    37981 aggtttcaag cagttctcct gacttattgt ggtaaatctt ctcttgttct cctaggctca
    38041 aatgcggtcg gtgagtggtt ttaaagtagg taaaggattg attcaaagca gcttcctgaa
    38101 ggcagacttg ggcctgacaa aagcttgtac tgagaggggt taatacttga cgattgactt
    38161 ctatctctcc agatttgcta aggctgattc gcaagcggta atctcgatta gcttcacaat
    38221 cctgacactc ttcctcaatc ttgtgtccca agtcttctgc atcaaaagga aaagcaaaat
    38281 aacgactagc ttttctcagt ctttccagat gttgatcttc aaacagcagg ttcttctggc
    38341 tgatttcccc tgtcgtaatc agttggaagc gagcttgttt acgataaaga actgctgcct
    38401 tttgatgaac ctctcggtat tcagattccc atgtgctatc ccaagtaatc cctccgccaa
    38461 ctccataaat ggcttgacct ttgtgaagtt gaatggtacg aatggccaca ttaaaaatcc
    38521 gtcgtccatt tggaagcaag agaccaatcg ttccacagta gactccacgc ggttgaggct
    38581 ccaagtcctt gataatctcc attgtcgcaa ttttcggtgc acccgttatg gaaccacaag
    38641 gaaagagtga gcggaagatt tcaacaaggt ccacatcctc tcgcaactga ctcttgatgg
    38701 tcgaagtcat ctgccaaaca gttgaatact gctctacctg acacagacgc tccacgtgct
    38761 cgctcccaac ttcagaaata cggttcatat cattgcgcaa gaggtccaca atcatcatat
    38821 tttcagagcg atttttggga tcctgttcca accaactggc ctgttcaaga tcttcttggt
    38881 cagttacccc acgctgagtc gtccccttca ttggtcgtgt tgtcaactcg cgatcatttt
    38941 gctcaaaaaa gagctctggg ctcatggaaa tcactgccat ctcgtcatgt tccacatagg
    39001 cattgtagcc cgcctcctgc tctaccacca tacgattgta gatggcaaaa ggattggcac
    39061 ttaacttttg cttgagttgg acggtgtagt tgacctgata ggtgtctccc tgcctcaagt
    39121 gatggtgtat ctgggcaatg gccttttcat agtctgctgc agacgtcact tcctgccaat
    39181 ttgagggcaa atcaatatcc tcataagtca gaggaatagg ggaagtttct acgctatcat
    39241 gaacagtaaa gtaaagcagg tactctccca gtaggggatc cttgtgaact gctaattttt
    39301 cctcaaaagc aggtgcagcc tcgtagctga cataccccac cacataataa ccttgctctt
    39361 ggtagctttc cacttgtgcc agcaaatctg ccacttcttc tacatttctc gttttcaact
    39421 ctttaatagg ctgggcaaag gtatatctct cccctaaagt cctaaaatca atcactgttt
    39481 ttctatgcat accttaagta tagcataaaa aggcagatac agacaaagct gatctacctt
    39541 tttacgattt tattcattct atgcttgtgg tgcagtaaat agatcaccag agaaaccgtc
    39601 tgtgttttta aggagagctt taaagacttt cgatttcaag acaactgtat ttcggtattt
    39661 gtctttaata ctactatcgt ttagttccgt taaatcttta gtaacagcct catcaaacat
    39721 agtcttcaac tgttcatagg ttgaaatttg agtgccatta atttcaattg gtgcaaaacc
    39781 ttttttagat tttgtaagaa cttccccata ccattgcttc ttccaatctt caaggtttga
    39841 aaattggtta ttagaaactt tcttaataat gaaatcatca cctaatagac cgttattttc
    39901 tttattggct gcaccttgat gtttgcttga aacatagtca atgaacccat gttcatatcc
    39961 atagtaaccc cacatacgga aggcgttatg tttgaaggat agagaaccag gggcaccttg
    40021 actcgtatta ccgccataaa taccagccat catattaaca ttgacatatg ccgtaccaaa
    40081 gtcctctggg tggaagacct tattacctgg atttccgtga atggtcatga aattattatt
    40141 taccaagtca tcgatggttt caaggttagt tgttttctca gcgtctatca aatcacgcac
    40201 tttgtcccat tggtgaggac cacttagttt atttctgtcc attggtcgac gtgtttcgcg
    40261 atcaattttc ttgaaccact tactattgtc acctttaacc ttaggaagaa cagcctcagc
    40321 ttctgcataa tcgagcatca tcatagcttc attgtagttc ttcatgtaat ggtcgatttg
    40381 gtcacgtgtt tgaagtttgg taggatctgg attataccat tggtttccat catttgaacg
    40441 ttcaaatgcc atatttaatc caagtgcgcc atattcacct tgatgtccat gttctgctgg
    40501 agtttgtaac ataccttgag caaatgcttc aagatctgta ccctgtcgat gtctatgacc
    40561 tcccagatat gccattcggt catttacgtg ggtagtctca tgtgtaaagg cagatgtgcc
    40621 atattcactt attaaatccg acataacaaa atatacagca tccataggct gtggatcagc
    40681 aaagatgtaa gcatacgctc catacccata cttataatca tgatacttat caatacgtcc
    40741 aaagaattca cgaataggtg cataatccgg tttatctttt cgatgaccaa atctatcagg
    40801 ccaaccaccg ggtgcaccat gattatccca tacaggagtt ggaactacat tttgactctt
    40861 aagaagttta tcgcggacat tatcagtggc aaggcgtgac caaaaatcta agtaattaac
    40921 ttgctcttta gcacgtttat tgatttcact cttgaacgat tcacgttttt cttctgtatc
    40981 tttaccatac ttttcaaacg aactaaatga gatagtatta tatgttgaaa tcataaataa
    41041 atgtgcatct ttcaagttca gaagcgataa aatcatgcgt ccatgttgtc cgttattaag
    41101 tccttcatat agacgatatt ttttattagc aaatgcaggg ttagttgacg gttgttcaac
    41161 tacataagca ttattttcaa tagctttctt aaaccaaaca tttatgtctg tttcatttgt
    41221 gaatagtttc atattgtagg tcaagaagtc atgtaagttt ccagaaagag tatttttcgc
    41281 caccacttca cggaaggact cctgtgtacg acttccctta atatagtcct cttttgaacc
    41341 aatttctatt aatcgatcta aaacacttac attcttacca taaaaatctg gtttgaatag
    41401 cattaattct ttaatattga agttatcaaa tttcacaccg taataacgat ttaggtatgt
    41461 taatcctaac aggacagcag ctttgttatc ttcaattttc ttaatcaagg cacgttttgc
    41521 agcttcatct gtattcagtt gatgatcttc attttcaact aattttgtca caaatgtatt
    41581 aagatgatct tttacttctt taaagctttc ttctaagtaa agattcttaa ttgcattaac
    41641 tctgttatcc ccagttcttc ctaatcgttg ataaattgga tcagattgta attcaattgg
    41701 acttaaaata ccttcaatag cactaattaa atctgcacga tctttaacaa ccatatttgg
    41761 tgtatagacg acttcaccaa gctctgcgac actgtattct tttacttgct taacttgaga
    41821 gtctttagga gaaatcgtaa agatatcttt tgtcttatct gcgtaatgaa ccataatatg
    41881 gtcagcatct gtaaggtctg taataaagtc attacctttc attgcagtca cagacaatac
    41941 ttctttattc attaatggac tatcagttgt aagcttgttc ccttgattca caatccattc
    42001 tttgttgtag aacggttgga gtttttcaac attacgataa acaagctcac gatttgcatt
    42061 ataatcttga attgttttgt attcttcatc acgtaatata cggcgattca acttatcaac
    42121 caaatgctca gttacttcaa atgtatcagc agtgataccc atatccttaa ttttcttatc
    42181 agcttctacc tgtgaaattt ctttaatttt agacgattgt ttatgggttt tatctccaga
    42241 actcacaccg ataactgttg cattattagg tttaatccaa ttgcttacaa attcatcatc
    42301 catatcatta gaaccaaaga aaatatcacc acgcttaaca ttcactaagc tgatattgtt
    42361 atcaatggtt ccccaaggcc agtctttacc aattaaaccg cctacatcac ctctagcaac
    42421 atcaatagta cctttggcta cagaatttct tacattagac caagcaccaa catctcttgg
    42481 attaaaatca tgagctaaat gtcctactat agcacctgca cgagtattta aacctttaag
    42541 ttcaacatct gcataagctt tatttaaatt gcctcgataa atctcagatg caatcccagc
    42601 caagttccac cctttatcac caacagattg aacttttccg ataaaagcaa cattagtcat
    42661 aatacctgca tcatctaatt tatccaaaat acccgcaaca ttgttattac caacgatagt
    42721 acccgttact ttaatatttt caacagttgc atttttcact gtttgagcta atgaagctaa
    42781 ttgatttgcc caaggcatat taatattaac gttaattaaa ttaatatttt tgatagaacc
    42841 cccttcaact ctatcaaata atggacgagc agtgttatga atggagaaat gcttaccatc
    42901 aacacttgtt aagtgacctg taaatttacc tgttacatat gatttacttg gtgtaggtac
    42961 atttgttgca tttaaatccg atcctaattt aaactcaccg ttaggatttt tctgcatagc
    43021 atctactaaa tctttgaagt tatagtaaac gttattttgc gatgctttgg gtttctctat
    43081 gtaataagtg tatgtttgac tcaaactatt atttgaatca cgttggatta aatcaggagc
    43141 tgaagcagta actttaaata aggatgtctc ctcctcagtt acttcctcta tattagtaat
    43201 ggcaagcctt gttactttat tatgattagt ggtaattttt agataatatg acgttagatt
    43261 ttctggaaca gtttctagaa ggctactatc tgtttcattt ccattctcat ccactttcat
    43321 caatcttgtt tctttaatat ctttaatctc aactttttta aggtctaact gaacttcttt
    43381 atcttctaat atttcagttt cttcaccgtt acctcgatca tagaccatgg ttgtggatag
    43441 tgtataacct tgataatatt tcaagttgtc taaagtagct tgtaagttat tttcttctac
    43501 cgttacttct ttaacaactg ttccagtatt atcttttaga gctactttaa tcgatttaat
    43561 ttctactcca gctggtttag tcaatgtata actagctgtc gcactcttct tcaagacatc
    43621 tttatctgtt ttagcccatt ccaaggttgg tttctctaac tcttttgtgc cattcttaat
    43681 aattttgtct tgaggttttt caatcactgt ttctgtgata gtaggagcgt cacttgtttt
    43741 ttctccgcga attgttttat aggtttcagt aatttgtttc ttatcgttta caccttcttg
    43801 agttacttgt cttgttccgc gttttaaagt gtcatcttct tgttcttttg tttcaaaagg
    43861 aatgatttct tctcttgtct caaccgttgt cccttcaatt ggttttgtac cacggattac
    43921 ctgttgattt acaggctcct tggtgacttc cgtactggtt gaaagaactt ggtcagtttt
    43981 aacccctcct attgttttat aggttgtttt tgtaacttga cttccttttt caccatttcg
    44041 taggacagtt tcttcgtccg tgtacttggt tgggtcctca gtcgtttcag ttttatagtc
    44101 aagttctgta gttgattctt caacgagtgt tccttcagtc accttatact caggtaattg
    44161 ttctggaaca agagcctcac ccacgtaatc ttcttcttgc gtccccttag ctgttttagt
    44221 ctcctctttt ggttctacag tttcttcagc cttggtatct ggagttgact cttcttgttt
    44281 cggtgtctct tccgctcccc tatcttcttt aggattgtct tcttgtgatg attcttttgg
    44341 tgtacttggt tgctcagctt gtgtaggttg cacgggttgt tctgggactt ttgcttgttc
    44401 tactggtggt tcaacttttg attcctcagc tggtttgtct gatggttgac tttctggctt
    44461 aactgctact ttttcctctg attttgactc aacttctcca cctacttctt caactggagc
    44521 tggttctgct gaatcttctt tcccctcttc tactttagga agggtgtcgt cagtaggttt
    44581 tacctccgat tttggttctt cctttggact ttcttctgtt ctaggtgctt cttcttttgg
    44641 agtttcctct gtctctacta cttggttttc tgtcctagct tgctcctgat ttgttgttga
    44701 ttgaggagtc tcaacttcga ctacagtcac ctctccaggt tttgctgagg tttcttctaa
    44761 aacagtgtcc aagccaagcg ttttgaggat gtcacctgat agataaccaa catagcgata
    44821 gccatccatt tcaacaacac cctctcgact agccagcgct aaggtcgcaa ctgggtctac
    44881 agcccctgca ctaggaagaa ctaccaatcc catagctcca actagaaaga cgctagcaat
    44941 tttctttctc ttgtagatta aaagcaagct cccaacagtc agcaaaccaa aagctgtcaa
    45001 aacagatgct tctgtccctg tttgaggcaa ctgatctttt tgatacacca aaccatatac
    45061 aacttcattc ctgtcaggct ttcctgtctg aattaaatct ttagcttctt gtgaaataat
    45121 ctctttattt acatagtgat aggtagctgc gtccactaca gaaggagcca tcaaaaggct
    45181 tccaagaaat acagagccta caactccctt aatcttacga attgaaaaac ggtctttttt
    45241 aaacactttc atctccttta ttcattctca agactttcta atagcatctt gcggatagtg
    45301 cgcacgcgca cctccgatta attttggacg actagccagt gccgttacat gggcatgacc
    45361 aatctctctc aaaatagggc gaatcggaac ctgaacatgc ttgacatgca tgccaattgc
    45421 agtgtctccg atatccaatc cagcatgagc cttgataaat tcaacctcaa ctggatcctg
    45481 cataaactta aaggctgcca actgccccga acctcctgca tgaagagtag gatggacact
    45541 gacaatttcc agatcaaact gctctgccac ctgacgttca acaacgagag cccgattgac
    45601 atgctcacaa ccttgaactg ctaaatggat acctctacta cctagaatac ccaagatagt
    45661 ctccactatc agctcaccaa tctcttgact ggattctttc ccaatctgac cacctagcac
    45721 ctcactagaa gatagaccta aaacaaaaag gaccccctgc ttcaaattgg tcttttctaa
    45781 aacatcttcc actacctgac gtgtttctct ttgaatctgt gtctcgttca tctctgttac
    45841 ctctgttgtc actcttctat cataccgttt tttcttgttt ttagcaagat agacaaccta
    45901 taaagtctgc ccaattacgc ataaaactcc cagaattgac tgggagttag ctagtttcta
    45961 ttctatttat atatatttca actttcgttc ctttttgggg tctagaatca atcttcatct
    46021 ggtaattggc tccaaaatga agtttgagcc gttgatcgac attttgaaga ccaactcccc
    46081 cacgtttgag ttgactttga ctactatcac cagcatcttg gaagccaacg ccatcatcct
    46141 caatacggat gaccaatccc gaatcctgtt tctggacaga aagtttaata tggccctgac
    46201 cttccttttc cttaatgcca tggtaaagag cattttctac aaggggttgt aggaccagct
    46261 tgggtaagac taaattatca aaggcaacat tttcattaat ttcgtattcc agcttatctc
    46321 catagcgttg tttctggata aagagatact ggcggacatg attgatttcg tcagagagac
    46381 aaatcaagtc cttgccttga ttgagcgcca agcggaaata ggttgccaag gacttggtca
    46441 cctgcaccac tcgctgacta tcatgaaatt cagccatcca gatgatggtg tccaaagtgt
    46501 tatagaggaa atgtggatta atctggctcg aaagggcttg aagttggtac tgacgggtcg
    46561 tttcttcctg gctacgaata gctaccatca actgatcaat ctgatccaac atagcattaa
    46621 attggcgagt tacttctctc agttcatagg caccaacttc cttggcacga agattttgag
    46681 caccagaagc aatttccaac atggtttctc tcaaatcctt caaaggagca atccagcgtt
    46741 taagactgaa ccacactaag cagagacaaa caagaagaga tgtgacactg gccccaagca
    46801 aggtccacaa gagctgactc cgaacctggt ctaacttttc caatgatgac acgccaagta
    46861 ccgtccaatc agttcctgca atcttctctt gactgacgta ggatttgtga ccaggagtat
    46921 aaccctgacc tgtctcgatg tagggtttca tagcctccat tttgctagac gaactataaa
    46981 ctgtgtgttg aggatggtag acaaattcat ggttttcatt gataatgaag gcaaagccct
    47041 gctgccccaa ctggagttga ttgagatagg cttccagagt ttcataagaa atatccaaac
    47101 gaagcacacc aagattggct ccctttgcat caacaagttc ttgagtgaca gaaatgaccc
    47161 actgactatc tgatttacga gctggagtca aaacaggcat agctccctga tgaatagcct
    47221 tttggtacca atcctcagcc atcatatcag aggaagtttt catctgcaca ctgtcatctg
    47281 tagaaatgac ctgaccagat ttggtcacca gcacaacagt tttcaagtcc ttatctgact
    47341 tcaagatggt caaaaacaaa tctcggattc cctcgacctt gtcttgactg ggattctcag
    47401 cataggccag aacatccgtc tgctgggtca aaccagtcga ggtggtttct agttttttga
    47461 tataagactg aataaagtgg ctagtctggc tgatggtcgt ttggctgttg ccctcaatgg
    47521 tggcctcaat ggctgaagaa cttgattgat agtagaaagt tccaaccaga gctaggagaa
    47581 tgagaaagac cagaaagatg gaaataacca ttctaactaa aagagaagaa cgcttcatcg
    47641 gtcttctccc ttcttaaact gacgaggtgt cacacctgca atctgcttaa aacgttgggt
    47701 aaaatagttc atatcttcaa aaccaacctt ctctgcgatc tcataaatct tcagatctgt
    47761 agttaaaagc aagagcttgg cttgtttaac acgttctctc accagataat cctgaaaagg
    47821 caagcccaac tctttcttaa tcaaggaact cagataggtc ggactaaaac ctaagtcact
    47881 ggctaaagac tttaaactaa attggctatc agccagatga gactggattt tctgggccat
    47941 gtttccttca aacctattag tcaataaatc ttgtaactgc tcttctttct cttccttgtc
    48001 tagtttttgt ttgattttcc ccaacatttc ctcaatatcc tgacgagaaa agggtttgag
    48061 caggtagtcg tccacaccta gtttgacagc agacaaggca taatcaaaat catcgtaacc
    48121 tgttaaaaag accaaatgaa cctgaggata ggtttctcgt accagactgg ccaactggat
    48181 gccatttaga tgaggcatgt tgatatcggt taaaatgata tctggcacct gcttttggat
    48241 caattcccaa gcctgccttc cattttcagc ctgaccgatg atttccatat cgtaggctgc
    48301 tacattgacc agtttagtca aaccttgtct taccagatat tcatcttcta cgattaagat
    48361 tgtgtaggtc atgctctgct cctttaccac ttactagtat cagtatagca aaattctcct
    48421 ctaactgctt aggaaagacc tcttatactc aataaaaatc aaaaagtaaa ctaggaagat
    48481 agccacaggt tt
//
LOCUS       DAVBCW010000005        47768 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVBCW010000005 DAVBCW010000000
VERSION     DAVBCW010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 47768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47768
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.005"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(251..511)
                     /locus_tag="VQ514_000228"
     CDS             complement(251..511)
                     /locus_tag="VQ514_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499050.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDITRLTASLLGDEKLSAITSSSA
                     VKADIHRFVIELEPVKSTILQNDDISLDQNEIFEILKNFLDG"
     gene            complement(570..1121)
                     /locus_tag="VQ514_000229"
     CDS             complement(570..1121)
                     /locus_tag="VQ514_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499051.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIYEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNPEFPSMIELFSIL
                     PEYPLKKDGREIPLHFDQDASLSALLLDEDYYNILVHEKETIQGYSVLSNCGLYSSKI
                     SSNHVSFHLQPQNSVLSSLQLAS"
     gene            complement(1111..1308)
                     /locus_tag="VQ514_000230"
     CDS             complement(1111..1308)
                     /locus_tag="VQ514_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499052.1"
                     /translation="MLEIWKYRPFVSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRI
                     EEESEALENMILQYLGEDDAS"
     gene            complement(1312..1896)
                     /locus_tag="VQ514_000231"
     CDS             complement(1312..1896)
                     /locus_tag="VQ514_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499053.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEIPKELTPSEQLTWIAFLLPDGDIKQIKSVSNLLYGGAYALS
                     HSTFLAETDENTSYVIWQRKFNQLSLPLSQHVLK"
     gene            complement(2304..2729)
                     /locus_tag="VQ514_000232"
     CDS             complement(2304..2729)
                     /locus_tag="VQ514_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499054.1"
                     /translation="MTSRVFIDADCISAFLWVGTEHLLEKLYSGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVTKGSAEIVSIDIGTEEYALYRDLTRNYDSNKIIGKGEATSIFLA
                     KKHNGILGSNNLRDVKPYVEEFSLEHMTTGDILIEAFKA"
     gene            complement(2722..3216)
                     /locus_tag="VQ514_000233"
     CDS             complement(2722..3216)
                     /locus_tag="VQ514_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499055.1"
                     /translation="MKKSSVSLILIGEGDETERKADQFASYFLIFPSSLYRMVEEIRE
                     NANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIETASRLGYD
                     TSLYRPLSESKKEMVLGHYIKSTEQLLENNRISQGKYEELLLDAFRYDIVYGLDEEGE
                     LSFD"
     gene            complement(3262..3468)
                     /locus_tag="VQ514_000234"
     CDS             complement(3262..3468)
                     /locus_tag="VQ514_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499056.1"
                     /translation="MDLSNKASNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMPLGR"
     gene            complement(3455..3616)
                     /locus_tag="VQ514_000235"
     CDS             complement(3455..3616)
                     /locus_tag="VQ514_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2499057.1"
                     /translation="MNMINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
     gene            complement(3981..4937)
                     /gene="lytA"
                     /locus_tag="VQ514_000236"
     CDS             complement(3981..4937)
                     /gene="lytA"
                     /locus_tag="VQ514_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HET2499058.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPDFTVEPEGLITVK"
     gene            complement(5313..6683)
                     /locus_tag="VQ514_000237"
     CDS             complement(5313..6683)
                     /locus_tag="VQ514_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET2499059.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYHLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
     gene            complement(6990..8156)
                     /gene="recA"
                     /locus_tag="VQ514_000238"
     CDS             complement(6990..8156)
                     /gene="recA"
                     /locus_tag="VQ514_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HET2499060.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVPLDLGDELEIEIEE"
     gene            complement(8211..9467)
                     /locus_tag="VQ514_000239"
     CDS             complement(8211..9467)
                     /locus_tag="VQ514_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HET2499061.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNLLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQVDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGISSIFKGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            complement(9551..10567)
                     /locus_tag="VQ514_000240"
     CDS             complement(9551..10567)
                     /locus_tag="VQ514_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET2499062.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNVERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEKALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSVGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            complement(10575..11093)
                     /locus_tag="VQ514_000241"
     CDS             complement(10575..11093)
                     /locus_tag="VQ514_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2499063.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            complement(11083..11526)
                     /gene="tsaE"
                     /locus_tag="VQ514_000242"
     CDS             complement(11083..11526)
                     /gene="tsaE"
                     /locus_tag="VQ514_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HET2499064.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            complement(11612..12232)
                     /locus_tag="VQ514_000243"
     CDS             complement(11612..12232)
                     /locus_tag="VQ514_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499065.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            complement(12615..13478)
                     /locus_tag="VQ514_000244"
                     /pseudo
     CDS             complement(12615..13478)
                     /locus_tag="VQ514_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            13643..13837
                     /locus_tag="VQ514_000245"
     CDS             13643..13837
                     /locus_tag="VQ514_000245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499066.1"
                     /translation="MKKKRVSRMKKNRGIQKLAILVLLGVFMFSNTIPYQQFIQKNRQ
                     LEIRVQSQKKSNGLDVGKAD"
     gene            14152..14448
                     /locus_tag="VQ514_000246"
     CDS             14152..14448
                     /locus_tag="VQ514_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HET2499067.1"
                     /translation="MLKFRKVVASSLAVLAFSLALPVLAVSHRGGEWTYGGHHDPNNW
                     GAFSNYYHGSQYHWAYVGSNGRNNQKTVYAGARSAAYAFVNTNFGEQVTFDAGW"
     gene            14551..16566
                     /locus_tag="VQ514_000247"
     CDS             14551..16566
                     /locus_tag="VQ514_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HET2499068.1"
                     /translation="MKKLFILISNLLASLFFVWVFTIWTDTYVSYYYPNVVVHDSSPE
                     TTFQHVATRLEKLAEETDSFIAIQHQDPNSEGTTVFSYTTFGDGKLPDGLQEKKLEDA
                     QSSSVETNYFVFDGHLDIHLLREELSQLGLTNMNLTIPSKLSTLMAIFSNGFQLISLL
                     IFILTFIALTLISQISQLRSSGIRLISGEKRWSIFLRPVGEDLKGIAVGFSLAGVLAI
                     LMQKILSLPTQSLMTIGAGLLSYNLILLSISLFFAQLFAVGIKKIHLMQIIKGQVPVR
                     GIISLILIGQLLAIIIVTLGIGSSLKYSQAWQQHRIGQEAWSQERQLITLSISREGTS
                     PGFDEQAQRKLRTWYQLMDLAVSEQKAFLSRHQLIDRTLQNGMASSKNLITSTEWHDY
                     NPNGNVLIVTPQYLERQNIPVDTTIEQKMNHLDVGEFVLLLPEHLRSEEEHYKSVFED
                     DLTSRMSSQDERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQ
                     SILFWIDAVQNYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVM
                     LAGAGLGIATSILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLG
                     FVASVFLQVEIGVAFLVLLLFTGLSLLQLHVQMQKENKMSMLVLKGG"
     gene            16568..17200
                     /locus_tag="VQ514_000248"
     CDS             16568..17200
                     /locus_tag="VQ514_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2499069.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLTYLDLDKRIFELSGGESQRVALAKVILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDRLK"
     gene            17341..17619
                     /locus_tag="VQ514_000249"
     CDS             17341..17619
                     /locus_tag="VQ514_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComA"
                     /protein_id="HET2499070.1"
                     /translation="MKKFDNYIVEKPCDSNSDKLQKILIIENLVDDILQFPLRINNIV
                     GEIFLLQPFQKKTIFIPCYFEEDIVKVKDDDKVEWNLLEFQKFRAFLA"
     gene            complement(18128..18541)
                     /gene="ndk"
                     /locus_tag="VQ514_000250"
     CDS             complement(18128..18541)
                     /gene="ndk"
                     /locus_tag="VQ514_000250"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HET2499071.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEVIQNVVHGSDSEESAKREIALWF"
     gene            complement(18652..22329)
                     /gene="rpoC"
                     /locus_tag="VQ514_000251"
     CDS             complement(18652..22329)
                     /gene="rpoC"
                     /locus_tag="VQ514_000251"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HET2499072.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKTEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGEVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPYAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            complement(22362..25973)
                     /gene="rpoB"
                     /locus_tag="VQ514_000252"
     CDS             complement(22362..25973)
                     /gene="rpoB"
                     /locus_tag="VQ514_000252"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET2499073.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVVAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLKEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            26388..26458
                     /locus_tag="VQ514_000253"
     tRNA            26388..26458
                     /locus_tag="VQ514_000253"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26420..26422,aa:Cys,seq:gca)
     gene            complement(26656..27987)
                     /locus_tag="VQ514_000254"
     CDS             complement(26656..27987)
                     /locus_tag="VQ514_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HET2499074.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPVIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFNVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(28278..29102)
                     /gene="endA"
                     /locus_tag="VQ514_000255"
     CDS             complement(28278..29102)
                     /gene="endA"
                     /locus_tag="VQ514_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HET2499075.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSTANSPKTKLSQKKQ
                     APEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            complement(29141..29329)
                     /locus_tag="VQ514_000256"
     CDS             complement(29141..29329)
                     /locus_tag="VQ514_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET2499076.1"
                     /translation="MNKKSSYVVKRLLLVIIVLILGTLALGIGLMVGYGILGKGQDSW
                     AILSPAKWQELIHKFTGN"
     gene            complement(29322..30605)
                     /gene="murA"
                     /locus_tag="VQ514_000257"
     CDS             complement(29322..30605)
                     /gene="murA"
                     /locus_tag="VQ514_000257"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET2499077.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            complement(30629..30859)
                     /locus_tag="VQ514_000258"
     CDS             complement(30629..30859)
                     /locus_tag="VQ514_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HET2499078.1"
                     /translation="MVQLLFTLSSHMLFIYVSFYLLKDLVRWEKVLKVTAENTRKVRV
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            complement(30917..31954)
                     /locus_tag="VQ514_000259"
     CDS             complement(30917..31954)
                     /locus_tag="VQ514_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET2499079.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            complement(31938..32426)
                     /gene="coaD"
                     /locus_tag="VQ514_000260"
     CDS             complement(31938..32426)
                     /gene="coaD"
                     /locus_tag="VQ514_000260"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HET2499080.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLCVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATCLVRGLRNASDLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACYVPESILEEIRNEK
                     KD"
     gene            complement(32416..32955)
                     /gene="rsmD"
                     /locus_tag="VQ514_000261"
     CDS             complement(32416..32955)
                     /gene="rsmD"
                     /locus_tag="VQ514_000261"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HET2499081.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEVGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAERELFSEDVMVVCETDKTVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            complement(33020..34012)
                     /locus_tag="VQ514_000262"
     CDS             complement(33020..34012)
                     /locus_tag="VQ514_000262"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HET2499082.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            complement(34124..34807)
                     /locus_tag="VQ514_000263"
     CDS             complement(34124..34807)
                     /locus_tag="VQ514_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HET2499083.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYLLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            complement(34863..35603)
                     /locus_tag="VQ514_000264"
     CDS             complement(34863..35603)
                     /locus_tag="VQ514_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET2499084.1"
                     /translation="MTIITSKANSVVKNAKKLHQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRNQLAAFPQTVWISEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            35637..35915
                     /locus_tag="VQ514_000265"
     CDS             35637..35915
                     /locus_tag="VQ514_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HET2499085.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            35993..36919
                     /gene="yidC"
                     /locus_tag="VQ514_000266"
     CDS             35993..36919
                     /gene="yidC"
                     /locus_tag="VQ514_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET2499086.1"
                     /translation="MKSIKRFAFSAMGVAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(36994..37788)
                     /gene="pflA"
                     /locus_tag="VQ514_000267"
     CDS             complement(36994..37788)
                     /gene="pflA"
                     /locus_tag="VQ514_000267"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HET2499087.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(37914..39164)
                     /locus_tag="VQ514_000268"
     CDS             complement(37914..39164)
                     /locus_tag="VQ514_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HET2499088.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     ISLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            complement(39231..40058)
                     /gene="purR"
                     /locus_tag="VQ514_000269"
     CDS             complement(39231..40058)
                     /gene="purR"
                     /locus_tag="VQ514_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HET2499089.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLHEFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            complement(40325..41266)
                     /locus_tag="VQ514_000270"
     CDS             complement(40325..41266)
                     /locus_tag="VQ514_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HET2499090.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMTNKIFAMDNRSFYKPDLD"
     gene            complement(41256..42512)
                     /locus_tag="VQ514_000271"
     CDS             complement(41256..42512)
                     /locus_tag="VQ514_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HET2499091.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVVVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFARDIRNKYI
                     APPRTTNFGILFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            complement(42514..43176)
                     /locus_tag="VQ514_000272"
     CDS             complement(42514..43176)
                     /locus_tag="VQ514_000272"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HET2499092.1"
                     /translation="MSEYKLSENNWTRVAVFAGGDRGHYRTDFDGFVGVDRGSLWILE
                     EDLPLALAVGDFDSVTEEERQVIQKRAQYFVQARPEKDDTDLELALLTIFKQNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLASYMHQIEIEDGQNLITYCPEGISQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            complement(43139..43795)
                     /locus_tag="VQ514_000273"
     CDS             complement(43139..43795)
                     /locus_tag="VQ514_000273"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET2499093.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDGHFVP
                     QISFGAGVVEALRPHSKMVFDCHLMVSNPEHHLEDFVRAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTPVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALRDEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGDVNERVQTLRKQLD
                     "
     gene            complement(43806..44684)
                     /gene="rsgA"
                     /locus_tag="VQ514_000274"
     CDS             complement(43806..44684)
                     /gene="rsgA"
                     /locus_tag="VQ514_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HET2499094.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDGGELDFYQQTYGDIGYDFVTSKEELLPLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     TAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGTIAPFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            complement(45277..46050)
                     /locus_tag="VQ514_000275"
     CDS             complement(45277..46050)
                     /locus_tag="VQ514_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HET2499095.1"
                     /translation="MRKKLVNSLMCAVVLSGFPIATYANEKNIGPNITVSNGQTRLSI
                     PEKNGVEVIQSEENRVVYEVKEAAKIETSDGDMVAPIVVDSRITSINNQVVQGKTVYT
                     ADLEKATFVEEGYNKLAKPTNILDHIFGIQKVHAYTQNITTHQSQFDSTYSVELYTVV
                     HWVSYDGGVQKNITKISGGVDFHERDIEIESSSVEVRQGGWYSSQQKWFHPGARSSWE
                     YSTGFSKVGPGENRVVTNYTVNLIRYGDRWSAYLQNNPW"
     gene            complement(46740..47612)
                     /gene="rsmA"
                     /locus_tag="VQ514_000276"
     CDS             complement(46740..47612)
                     /gene="rsmA"
                     /locus_tag="VQ514_000276"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HET2499096.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAEVMAFEIDHRLVPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPDLPIKVVANLPYYITTPILMHLIESGIPFSEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPVPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLE
                     EFASLADALKGQGL"
BASE COUNT        14048 a        10558 c         8824 g        14338 t
ORIGIN      
        1 atttgtcctg ttctcgtttc atttcactat actaaaaaac cacctgaaac aaatcaagtg
       61 actagaggat aaaaacaaag actaggcaaa aagttttgaa aagcaaaaaa cgcataatat
      121 caggtcttga aaaaccttga tactatacgt ttgattgtgg gaagattgac ttcattttct
      181 cctgaaattg aagttttttc tagtttccag atatagacga attcaatata gccatctcgc
      241 tacaattatt ttaaccatcg agaaaatttt tcagaatttc aaaaatttca ttttgatcca
      301 atgaaatgtc atcattttga agaatagttg acttcacagg ctctaattct atcacaaagc
      361 ggtgtatgtc tgcttttacc gcactacttg atgttatagc cgataacttt tcatctccta
      421 gcaaggaagc tgtcaaacgg gtaatgtcat tcaaatgctt tttaatggac ttacttaaac
      481 cttgagcacc tgtagcagag cgttccctca tatccagcca agctttagcc ttaaaaataa
      541 ttaatactca atgaaaatca aagagcaaac taggaagcta gctgcaggct gctcaaaaca
      601 ctgttttgag gttgtagatg gaagctgacg tggtttgaag agattttcga agagtataaa
      661 ccacaattac tcaataccga atacccctga atggtttctt tttcatgcac caatatatta
      721 taataatctt catccaataa taaggctgat aaactagcat cttggtcaaa atgtaaggga
      781 atttctcgac cgtccttctt taatggatat tctggtaaga tactaaatag ttcaatcata
      841 gaaggaaact caggattagt tgttgtaaat cgaaaaagct gcgctttctc atctttctga
      901 cttccttgat actctcccaa ttctaaaaaa tgattcaagg tagtataaaa ttccttattt
      961 tttacttcat aaatgatgac catatcataa tcttttgttg tgcgactttt aaatccttgc
     1021 gaatccaata cgatagaggt agcagttccc ccaatcagaa cataatagtt ctgaaaatcc
     1081 gcaaacattt cttgaaaaat aactttcgta ttagctggca tcatcttctc ccagatactg
     1141 taatatcata ttttctagtg cttcactctc ttcctctata cgtgggtcat catcatcttt
     1201 taaggtcaaa taaagagaaa tcggatctac aaattgttta tcatgattat ttttaaaatc
     1261 attccaaaac tcagatacaa aaggacgata tttccatatc tctagcatct ttcattttaa
     1321 aacatgctga gaaagtggca aggataactg attgaatttt ctctgccata tgacatagct
     1381 agtattttca tccgtttcag ctaaaaaagt tgaatgcgac aaagcataag caccaccata
     1441 tagaaggtta gaaacagatt ttatctgctt tatatcgcca tctggcaata aaaaggcaat
     1501 ccacgttaat tgttcgctag gtgttaattc cttagggatt tcagtatcat tcgcattgag
     1561 tactagtccc aatggaggga agaagaggtt tcccttaaag tctacaaacg gaactctagc
     1621 ttgaagtaat tgcttttttt cactgtctga taacttcgaa aacaccaaaa caacatccat
     1681 attggctttt tcacccatag tgcgagcctg agtaacaaat gaactcaaac tcccccttct
     1741 cttttcttta atcaataaaa atgattcctt atgaaaagtc atttgagtat actcaaacct
     1801 ttttacaaaa ctgattggta aagtcaaagt cagttcctca atactgacat caacataaaa
     1861 ggattgaaaa attgacatta tctttttgac attcatgttt tttccttatt ctatcatttt
     1921 tattatattt tatcattcgg tataataaaa tataagtttt ttgataaaat aaccaaaaac
     1981 tactcgctat cccctctcac attaccctac atatcgtttg acatgcgact gatagttcag
     2041 gaaaacttca aggagaactt ttctctcatc cactatgcag gacttactat ttcacttcta
     2101 ctcctatagg ctcaatttga cacttttctt tttcgaattg ctcatatgcc tcctgaatag
     2161 tgagttcgat ttatccaaat ttatttttgt ctattttgat gaatacttcc acgaagatag
     2221 tctgaaaatg aatttgcacc aattttcctt ctctttttaa gcatattatt ccagatatga
     2281 ttgccctctt gttcagtaat aaattacgct ttaaacgctt caatcagtat atctcctgtt
     2341 gtcatatgtt ctaaagaaaa ttcttctaca tatggtttaa catctcttag gttattactt
     2401 cctaatatcc cattatgctt tttcgctaag aaaatagaag tcgcttctcc cttaccaata
     2461 atcttgttac tatcataatt tcttgttaaa tctctatata atgcgtattc ttcagttcca
     2521 atgtctatgc tcacaatctc agctgaaccc ttagttacca actggtctat cctagatttt
     2581 aaatggggaa ttgtaggtat attgatttca tcatacacct cttgtggaat aacaatttta
     2641 cccgaataga gcttttctaa aagatgttca gtgccaaccc ataaaaatgc tgaaatacaa
     2701 tctgcatcaa taaatacacg actagtcaaa cgacaactcc ccctcttcat ctagcccata
     2761 tacaatatca tatctgaaag catctagtaa cagttcctca tacttccctt gcgaaattct
     2821 gttattttct aaaagttgtt cagtcgattt aatatagtgc cctaatacca tttccttttt
     2881 actttctgac aaaggacgat ataaacttgt atcatagcct aatcttgaag ctgtctctat
     2941 aacactaata tccatatttt taatttcttc tgcatcaagg tatccatcat ttctcaatct
     3001 atataacata gctttatgac tgataccata aaactgaccc aattttataa tatcttctac
     3061 ttcaagatga gttctattgg cattttctct gatttcctca accatcctat acagtgaaga
     3121 tgggaaaatt aaaaaataag aagcaaactg atccgctttt ctttcagttt catctccttc
     3181 accaatcaag ataagactga ctgaactctt cttcacctca tcataataaa gatgatacag
     3241 tccatgtgct aaagaaaatc tttaccttcc taatggcatg tctgaattga ctgcaatgag
     3301 actgaactga gttcctttat aacagactcc gctaatattc tttccgagtc cataaaatac
     3361 cagcgtcaaa ttttctatct tttgtaccaa tttaaaaata tctatcggcg attcaccatc
     3421 agctcccaac ttttttctaa gatttgaagc tttattactt aaatccattc gtgatattcc
     3481 tttcaccttt ttccattttg gcaatcatgc tttgattcaa agacaaatac tcagcaatct
     3541 tatcttgagt aagatttgaa gatattctaa gttcttttat tctttttcca acatcacata
     3601 catttatcat attcatacac ctctcataaa aagaatagca cattcttgtc ataatttttt
     3661 aaataaaaaa attatgacaa aacaaggaag caatttattg atgctactta aaaatctaaa
     3721 attgatgata ttaaacctat ttgatgagtt cctatcaaaa atcgtatctt caacctcaaa
     3781 acagtactta aagctatccg actcggttta cattgtcaaa tttagatttt atttgagcat
     3841 aactttctag tttgcttttt gatttttgtt taatatagta acaaaaattg aaggaaatat
     3901 ctccacaaga aaacgcatac tattaagctt tttcaagacc taataatatg cgctgttctg
     3961 atttgaaaga cattccatta ttattttact gtaatcaagc cttcaggctc tactgtgaag
     4021 tctggcttgt ctgccagtgt tccgtctggt ttgaggtagt accagcctgt tccgtccgct
     4081 gactggataa aggcatttga taccatggcg ccttctttag cgtctaagta gtaccaagtg
     4141 tccttgtact tgacccagcc tgtcttcatg gcaccttctt cgttgaaata gtaccactta
     4201 tcagcgattt tcttccagcc tgtagccatt tcgcctgagt tgtcgaacca gtaccagttg
     4261 ccgtctgtgt gcttcctcca gcggtctgca agcatatagc ctgaactgtc aaagtagtac
     4321 caagtgccat tgattttctc aaacttgtct tttggataag agccgtctga atgtacgtac
     4381 cagtagccag tgtcattctt ctgccagcct gtttcaatcg tcaagccgtt ctcaatatca
     4441 tgcttaaact gctcacggct aatgccccat ttagcaagat atggataagg gtcaacgtgg
     4501 tctgagtggt tgtttggttg gttattcgtg caatactcgt gcgttttaat tccagctaaa
     4561 ctccctgtat caagcgtttt cggcaaacct gcttcatctg ctagattgcg taagagttcg
     4621 atataaaggc ggtagtccgt catgaactct tctttggttg aatggctttc aatcagttca
     4681 accgctgcat aggtctcagc attccaaccg cccccaacgt cccaggcacc attatcaaca
     4741 ggtcctacct gcatgatgca accgttccca acaatgtgcg agaaaaaacc taattctggg
     4801 tctttccgcc agtgataatc cgcttcattc tgtacggttg aatgcggatt cccagttgag
     4861 tgtgcgtgta cttgcctata tggttgcacg ccgacttgag gcaaatctgt tcttaattta
     4921 ctcacattaa tttccatatt ctactcctta tcaattaaaa caactcattt tttacaatcc
     4981 agaaccagaa actcctttat ttctacctta caaagaagac aatcttagtc acgattaagc
     5041 ttgtagatag aacctcaaaa cgcactattt tgacactgta aataggactg acaaggtctg
     5101 cattctatct acaataacac cccagactaa aaagcttttc aaaagtatat ttttacagtc
     5161 tctatatgtc cttttcataa atactatact ttattatatc acataaaaga agtcaaaagt
     5221 ctgttaaact attttcaaca ccaaactaaa gaagagaaca caagtttttt cgatgttcac
     5281 tagaggaaat ggattttatt cagtaaatcc aactaggatt gcactttggt tgccaaaatt
     5341 gcctttcctt cttttatcaa gggatgacgg aatagtgaga agtacagttg aactgtcatg
     5401 gcaacccaga taagaggttc acaaaggata acacccttat atcctgccca aggaataatc
     5461 aaaaccacaa aaacgatttt tccgattagt tcaataaagc tagaaactag aggaaggatc
     5521 ttctgcccca agccctgcaa gcaattgcga taaatcaaca agaggctcaa aatgggataa
     5581 aaggctgaac tgatttgcag atagagactt ccattttcta tcaagtaacc atctgtcgaa
     5641 ctagccaaga aggaaaccaa ggctggactg gcaaaaaaga ggaaaataca aacaaaaact
     5701 gcccaggata tacttaaacg actgccgatt cgaagacctt gaacaatgcg gtcaggtcgc
     5761 ttagctccta gattctgaga agcaaaggtc gtcattgatg cagaaatagc ggtcatagga
     5821 agaagggcaa aggtcataat gcgtcgagct gccgtctggg cactaataat cactgcacca
     5881 aatgtattaa cagaaaactg taaaatcaca ctgccgatag atacaattga actcatcaag
     5941 cccatagcca aaccttgctc caagagatcc gcgtacaagc ttttgtccca tttgaaatgt
     6001 ttaaactgtg gcaagagttc tggcacactt ttacgaatat aataaaagca gagaaccgct
     6061 gataaacctt gcgaaataat ggtagcaagt cctgcggatt gaactcccag atgcaattgc
     6121 gtaataaaat agagatccag aaccacatta accaaggcag agaaaatcag aaatcccagg
     6181 gctgctagac tgtcaccaat agaccgcaac aagcctgcaa aaagattata agcaaagctg
     6241 acacctacac aggtcacaat catagaaata tattgataag attgaggaag aatttctgca
     6301 ggagtatcta agtattgcaa gagatgatac aagccaagaa agcccagcaa cataactaga
     6361 atgctcaaaa gagcacctaa aatccaagta gctgctactg cttccttgat tttagtgaaa
     6421 ttccgagccc cataataacg agcaatgaca atccccatgc cattgccaac accaagtgta
     6481 aaacctacaa tcaggtcaaa aatcgctgtc gtcgctccta ctgcagccaa ggattcttga
     6541 ccaagaaatc gtccaacaat caagacatca gcagtgttat agagctgttg aaaaatattt
     6601 gatagcaaaa ttggaaaggt gaagcttaag agcgagggaa gaatcggacc atgtatcagg
     6661 tccactgttc gttttttatt cataaagcta ttatatcagc tttccagagg agcgacaaga
     6721 aacagcaaac ttatatatag cggaatgaaa taaggaaagt caaatcgatt tttaacaatg
     6781 ttttagaagt agatatgtac tattctagtt tcgatctatt atagttggtt gagtttggaa
     6841 tagtacgctg tagctgctaa aacatttcta gaaattaatt tgactttcct aatagagttg
     6901 ttcatatctt atttcaagct actatatata atctaaaaac ttgaatcaaa aatcgaaaaa
     6961 gtagcggatt tctccactgc tttaacagct tattcttcaa tttcgatttc aagttcatcg
     7021 cctaagtcaa gcggaacttc ttcattcact gcttcttctt tctttggctc atcttttttg
     7081 ttttcagtat cttgttctga aacttcttct ccatcaatca agccaaattt agaacggact
     7141 tgcttatcaa tttcatcaaa gatttctggg tgctctgcca agtatttctt agcattctca
     7201 gaaccttgcc caattttttc atctttgtaa gaataccaag cccctgcttt tttgataata
     7261 tccaaatcgc ttgcaatctt caaaagctca ccagtcttag aaattccttc tccgtacata
     7321 atttcaacta cggcttcctt aaacggtgga gctaccttat tttttacaac cttaatctta
     7381 gtttctttac cgacattggt ttctttttgg tcaccagttc ccttaatttg tgtattacca
     7441 cgaacatcca agcggactga agcatagaat ttcaaagcac gtccgcccgg tgttgtttct
     7501 ggatttccaa acatcactcc aactttttca cgcaattggt tgataaaaat ggcaattgtt
     7561 ttggttttat tgatagaggc gccaagttta cgcatggcct ggctcatcat acgagcctgc
     7621 aaaccaacat ggctatctcc gatatctcca tcaatttccg cacgaggaac aagggcagca
     7681 actgagtcga ctacgacaag atcaactgca cctgagtcaa tcaattttcc cgcaatctca
     7741 agaccttgct ctcctgagtc tggttgagac aagagcaatt cgtcaatatt gacaccaagg
     7801 gccgcagcat aagctggatc aagggcatgt tccgcatcga taaaagcagc aatcccacct
     7861 tctttttgcg cttgtgcaac tgcatgaagg gcaaccgttg tcttaccaga tgactctggg
     7921 ccatagattt cgatgatacg tcccttagga taaccacctg agccaagggc aatgtcaaga
     7981 gctaaagaac ctgagctcat cacttgcacc ttttgctccg cacgttcacc caaacgcatg
     8041 attgatcctt taccaaagtc tttctcaatc aatttaagag cgtcattcaa ggccttttca
     8101 cgttctgccc caaatttttt tgaaatttct tctaattttt ttggtttttt cgccattcta
     8161 ttctcctaca ttctaatgat cctcagacct atctactatt atatcaaaaa ttagtcactt
     8221 aataaagcct tgcgaactag gttaaaggca tgcataaccg caatgtgacg tacatctgct
     8281 cggcttctgc ctccaatatt aaccttgata acctcagttc cttgatcttg cgccaagcct
     8341 atgaagactg tcccaactgg gtgtccttct aggctatctg gtcctgccac tccagtcaaa
     8401 ctaattccaa aatcagactg ggtcttgctt cgtgcctgct cagccatctt ctgagctgta
     8461 aattcagaca ccacaccatg ttcttccaaa ttcttggcag gaatatccaa catccttgat
     8521 ttttcctcca agctataggt cacaaaacca cccttaaata tacttgaaat tccagaaaaa
     8581 ttcgccacgg tagcttggaa aagacctgcc gtcaaactct ctgcagccgc gatggttttc
     8641 ccttgccttt tcagttcttc taccacaatg ctggctaaac tagtttcttc cccataacca
     8701 tagcaaaagt ctcgtaaaga aattccttcg aaagtctggc agtccaagat ttgattttcc
     8761 aagatatcca gcgcttgatt cgcctcttct tgactgctag cctttgttga cagacgtaga
     8821 gtgacttctc ctgtcttggc ataaggggcc aaggtaggat cgacctgatt atcaattaaa
     8881 tcagccaaaa tcgtaaccag ctggctctcg ccaatcccaa agaaacgaag aactcgggaa
     8941 tacagcttgc tccctgtcat caacttgggt agaagtaggt ttaagaccat gggtttcaat
     9001 tcacttggcg gacctggaag gacgacatag gtcactccgt ctacttctaa ttttcctcca
     9061 acagccagtc ctgtttcgtt tggcagtgga atcgctcctt ctacaatttg agcttgtctt
     9121 tcgttattcg gtgttcgggc atagtctggt cgcagggcaa aaaagatatc caacttctcc
     9181 tgagcctgag gatcaaagac taatgctttc cctaaaaatt tagctagggt ttgtttggtt
     9241 aggtcgtcct cagttggccc caaaccgcct gtcaaaatca ccagactgct acgttgactg
     9301 gcaatctcaa gcaaagacaa gagacgaact tcattgtctc ctacagccgt ctgaaaatat
     9361 acatctaccc caatctcagc tagtttttcc gacaaaaact gggcattggt gttgacaatc
     9421 tgtcctgtca aaatctctgt tccaacagca atgatttctg ctttcatgtt tcctcctacc
     9481 tatctattcg tatttttttg aaaaaatcgc aggaattttc ctacgattga ttttttattt
     9541 gtatcaaaag ttaattatct tcatcaccaa caggtgctct gccaaataaa tcttcaaata
     9601 aaaccgcatt ggtttcaagc tgagtaactt cttcttgtcc caaagaacgt cggagtatat
     9661 tttgcatttc caacatatgt gctcttgaaa caatctggta agaaacacct tgaagtatct
     9721 ctccttcacc ctgcaactgc tgagtttcaa tggttttaaa tgaatcttta tagcctagca
     9781 agttagggat actttttgca gacaaatcaa tattggtctg catattgtca ctcaaagctt
     9841 ttagaatctc ttgataatga ccaacgctat ttaaactgag agctttttcc atgacttttt
     9901 gaataacttc acgttgacgt ttttgacgac cataatcccc ctcaggatct tggtaacgca
     9961 ttcgtgcata gactagggct ttttctcccc caatatgttg ctccccaaca ccgatagaaa
    10021 tagtattaaa ttcttcttgg tcactgatag aaattgggaa acctaggata ttattgactg
    10081 taatacctcc tactgcatcc actagttttt gcaaccctct catattgacc atcacatagc
    10141 gatcaatatg gatattcatc attttttgaa tggtttctat agcaagctct gctccaccat
    10201 ctgcatatgc tgagttcagt ttcgcttcat gagcctgacc attccctgat tcaatgcgcg
    10261 tcagaatatc ccgctctaaa ctcatcattg ttgttttttt cgttttagga ttcactgtca
    10321 tcaagatcat gctatcactt ctaccgaccc aagtttcagt tcgttcaaca tttccggtgt
    10381 ccactcccat taacagaatg gttagaggtt cagtcgcttc aataaccttg gtttcttcgc
    10441 cgattttttt ataggtttta gctaaggttt ctgtcccttg ttgataaata gtataagcaa
    10501 aaacacctac tcctactaca gttacagaaa gtaaagctag caccattcca ataatttttt
    10561 taaccatatt tctactaacc tatcagttta cccatcaagt aaacatcgat aaatttccct
    10621 tcttctatat atgccccacg ctcttggcta ccttcaatga caaagccatg cttttgataa
    10681 agatggactg ctgcttgatt acgagtttgg acagtcagtt ggagacgacg cagaatgcca
    10741 cttgcttgtg cccactctat cgcttcttct agcaacaaac ttcccaagcc attattccaa
    10801 tatctttttc caatcacaat gaagagatct ccaatatgac ggactctctt acgctgatca
    10861 gctgtaatat ttacaatacc agcaattttg ccatttaaga atgcaagtaa ggttatctga
    10921 ttgtccgaac tagcttgctt gttgaggaat atttccatct cctcactagt caagagaata
    10981 ccatctccgt ctaggctggt aaagtctgtc tccaaactca cacgatttaa aaaggccact
    11041 aattcagctg catctttggg ctctgcttcc ctaatgagca attcatactc catattgaag
    11101 ctcctctaac aatttctcag cacgcaaacc ctttgcctga aaatttaaac ggcgtccatc
    11161 tgcttctttt agaatttcca attctaaata agcatctggc aaggcatctc ctaagagatt
    11221 tccccactca ataacagtca cgccgccacc aaagataaac tcatccaagt cgatagaatc
    11281 agcatctcct tcaatacgat aaacatctag gtgataaagt ggaagtcgac cttcatactc
    11341 tctcacgata gtataggtgg gacttttaat catttgagaa atctgtaatc cttttgcaag
    11401 tcctttagta aaggtcgttt tacctgcacc cagttctcca gttaagatta aaacatcatt
    11461 ctttgctaat agatggccca aacgctcccc taaggcttgc aactcttctt cattttttgt
    11521 gtacatactc ttattatacc aaaaactttt cttttgtgtc tattttccta ctaaacttat
    11581 catcataaca tccataaaaa acaggctttc tctaaaagaa aatgagcgta acaatgacca
    11641 atacaagatc tcggaaaata tgaccataaa aggaaacttc cttcttaacc gaatttggga
    11701 caagataggc tgcaaaaaac aagcccagtc caatataaat cagaagtgag acaatggtca
    11761 ttggatttct taagaaaaga agtgttgcta aaatagtcac caacactgtc ttttttctgt
    11821 ctagcatagc aagaaaatcg cgcacgtatt ttttcaaggg taaaaaaatc agcaaatcta
    11881 gcccaaatag gaaaaagaag gatggcaata aaaagtcaac taattcttgc tgcagcgtat
    11941 ttttgatgaa caagttatct gacaaaacaa gaacagctcc taacaaatta attaagagta
    12001 acatactgta aaaaagcttc accgacttct tactggctag gacactatgg acttcttgct
    12061 tacgggtata aagataattt actccagcac agattcctga aacgaaaacc atgctcccga
    12121 tgaaaaaagc tgtactttgt ttaaaggaca agatgcattc cttccatagg aaacagctac
    12181 tcaaactgat ttgaattaaa gctaacaaaa ataagattct cattgatttc atcttctctc
    12241 tcccttccta ccaatcatta tactaggaga aaagagagaa ctgtttctaa tcttctcaaa
    12301 tgtctcttta agacgctaaa caaacactag agactaatac tcaatgaaaa tcaaagagca
    12361 aactaggtag ctagccacag gttgctcaaa acactgtttt gagattgcag atagagctga
    12421 cgtgatttga agagattttc gaagaatata aatttgaaat catgaaaatc cgtcaaacgg
    12481 gtggttgttt tgtctcgcac ctcacggagc gagacggact cagagtcaca taattataag
    12541 gctgatagta ttaatctaac tatcagcctt acaggttatt taacgtttca gaaaaactat
    12601 aatgtcaaga ttaactaaac agtatctaat tccttcaaat aattttctat cttcatcagc
    12661 attaaaggat tgttataaat cttgcatagc tctcttgctt ctatataata atttttgact
    12721 tgttctctgt ctagaaattt ggctccagca tttcctacaa gaataagtag aggagccaat
    12781 tggtagcttg tctgtctttg tttacagagt tcgatagtct caagagcttc ttggatggct
    12841 tcattatatt tttcctttga tactaggtag tgagcgtaat tgtaacgaac tctgatgtag
    12901 ccaaataaaa actcttgatg ctcaaaattt tttgtctgat ataactccat caaatgagag
    12961 tagtttacct catattcttg ttcacgaccc actaaggaat agaaattaga tagagtattc
    13021 aacgccttta aataaatcaa agtatttgag gagactttta ataatatatt ttccaatgaa
    13081 gaaattgctt cacacttact gtcatattga cagaagtcaa taatagcttt aatccattca
    13141 aggtaagttc ggtcttctag tgttagaaaa gtacttcgtt caatctctat tctataaata
    13201 tattccaaat tttcataatt tctatcatct aataggcgag cagataattg cttgaaatta
    13261 gagaggttag atttaatttc aatttgttca ttaaaaaaat aatctaatgg cacttcgagt
    13321 cgttgtgaga gtttgaacaa aaggtctgcg gagggaatga aataccctct ctcaatttta
    13381 ctaatctggc tctattcaca aattccttct gcaagagttt gttgggagag tctcaactct
    13441 tttcttttca atctgaattt ctctgcgagt gtattcatta aagataataa accttcctat
    13501 ttgcttaatt tcattataaa atttttagtt caaaatagca agttaaagga ataaataatt
    13561 ctttgcggta ataaacaaaa aaaagtcaaa aattacataa aaatgcttgg ctaaataaaa
    13621 tatatttgtt aaaataagga taatgaaaaa gaaaagggtt tcacgaatga agaaaaatcg
    13681 tggaattcaa aaattagcta tattagtatt actaggtgtt tttatgttta gtaacacaat
    13741 tccttaccaa cagtttattc agaagaatag acaattggag attcgagtcc aatcgcaaaa
    13801 gaagtccaat ggtcttgatg ttgggaaggc tgattgattt tatattactt taaaattcaa
    13861 ctatttagag aaaggtattc tgatgagagc ttgtaacaat accagataga tataagaatg
    13921 gagatgtttg ataaaatgct ttcatagcta accaactgtt gaaaattatg agttacaaaa
    13981 tctaaagtat ttaagattgg tttataaaat taggtctctg cctgtagaaa ggaacgtgca
    14041 gttagaaagg aggaaaaaat aggatactat aattcagact gagatttcta attaaaatat
    14101 atagttgcat ttcgctcatt ttcataatat ttcattcaga ggagtaataa tatgttaaaa
    14161 tttagaaaag tagttgcatc atcccttgcc gttttggcat ttagtttggc tcttcctgtt
    14221 ttggcagttt ctcaccgtgg tggcgagtgg acctatggag gccatcatga tccaaataat
    14281 tggggagcat tttcgaatta ttatcatggt tcacagtatc actgggccta tgttggaagt
    14341 aatggacgta acaatcaaaa aacagtttat gcaggagcac gtagtgcggc ctatgcgttt
    14401 gttaatacaa attttgggga gcaagttaca tttgatgcgg gatggtaaag aataattgaa
    14461 gaagattctt ttatcaatag ttcagtaagc ttgttagttt atagagggca ggggtatttc
    14521 tgccctctgt tttagaataa ggaatgcact atgaaaaaat tatttatact catatccaac
    14581 ctgcttgcta gtttgttctt tgtttgggta tttaccattt ggactgatac atatgtctcc
    14641 tattattacc ccaacgttgt tgtacatgat tcttcccctg aaacaacttt tcagcatgtt
    14701 gctacacgct tggaaaaact agcagaagaa acagatagct ttattgcgat tcaacaccaa
    14761 gatcctaact cagaaggtac tacagttttt tcttatacga cctttgggga cggaaagttg
    14821 cctgatgggc ttcaggaaaa aaaactagaa gatgctcaaa gtagtagtgt tgaaacaaac
    14881 tattttgtat tcgatggaca cctagatatt cacttgctaa gggaggagct aagtcaactt
    14941 ggcttgacta acatgaacct gacaattcca tctaaactat ctacgttaat ggctatcttt
    15001 agtaacggat ttcagttaat cagtctattg attttcatct taacctttat agcactgact
    15061 ttaattagtc aaattagcca attacggtcg tcgggcattc gcctcatttc aggagagaaa
    15121 cggtggtcca ttttccttag accagttggt gaggatttga aagggatagc tgttggtttc
    15181 agtttagctg gcgtgctcgc cattcttatg caaaaaattc tgtcgcttcc aacgcaatcc
    15241 ctaatgacca taggagcagg cttgctcagt tataatctca tcttgttgtc gatctccctg
    15301 tttttcgctc aactcttcgc agttgggata aaaaagatac acctgatgca gattataaaa
    15361 gggcaagtgc ctgtcagagg aatcattagt ttgattttaa tcggtcaact attagcgatt
    15421 attattgtaa cgctgggaat agggagtagc ttaaagtatt cccaagcctg gcaacagcat
    15481 cggattggac aagaggcttg gagtcaggaa aggcaactga ttaccctatc aatcagccgt
    15541 gaaggaacga gtcctggttt tgatgaacaa gctcaaagga aactcagaac ttggtatcaa
    15601 ttgatggatc tggctgtttc agaacaaaag gctttcttat ctagacacca gttaattgac
    15661 cgtactttgc aaaatggcat ggcttcctcc aaaaacttga taacctctac agaatggcac
    15721 gattacaacc cgaatggcaa tgtccttatc gtcacaccgc aatacttgga gcgtcaaaac
    15781 attcctgtag atacaactat tgagcaaaag atgaatcacc ttgatgtagg ggagtttgtc
    15841 ttattgctgc ctgaacacct ccgttcagag gaagaacatt ataaatctgt ttttgaagac
    15901 gacttaacca gtcgcatgtc tagtcaagat gaacgacagc aaatgactgc tacggtaggt
    15961 tatttagaat caggtcagga tcgttttgtg tataatacga cccctatttc ttaccagcag
    16021 tttttgaaag atccaatcat cattgttata acaccccaat caactggtcc acagtccatt
    16081 ttgttttgga tagacgcagt acagaactac gttctcttta atcaattgtc tgatgcccag
    16141 gagcttatcc agagacaagg cattgaaaat tgggtctcag aaatgcaaac aggttaccac
    16201 aactacatca cattattgga taatattcag agggaacgtt gggtaatgct agcaggagct
    16261 gggcttggga ttgcaacttc aatcttgttg tttaacacta tgaataggct ctactttgaa
    16321 gaatttagac gtgccatttt tatcaaacgc attgcaggtc tcaggttctt agaaatccat
    16381 cgcacttatc tctttgctca actgggtgtg tttttactgg gatttgttgc gagtgtattt
    16441 cttcaggtag agataggagt tgctttctta gtcttgttac tctttactgg tctatctctt
    16501 ttacagttac atgtccaaat gcagaaagaa aacaagatgt ccatgcttgt tttgaaggga
    16561 ggttaatatg attgaactta aacaggtgag taaatctttt ggagaacgag agttattttc
    16621 gaatctttca atgacatttg aggctggaaa agtctatgcc ttaattggtt caagtggtag
    16681 cggaaaaaca accttgatga acatgattgg gaaattagaa ccttatgatg ggacgatttt
    16741 ttaccgaggt aaagacttgg ccaattataa atcgagtgat tttttccgtc acgaattggg
    16801 ctacctcttc cagaactttg gcttaattga aaaccaaagt attgaagaaa accttaagct
    16861 aggtctcatt ggtcaaaagt tgagtcggtc ggaacagcgg ttgaggcaga agcaggcttt
    16921 agaacaggtc ggcctgactt atcttgacct agataagcgc atctttgagt tatcgggcgg
    16981 agaatcgcaa cgggttgcct tggcaaaagt tatcttaaag aatccaccct ttattctggc
    17041 agatgagcca acagcttcaa tagacccagc aacttctcag ttgattatgg agattctgct
    17101 atctcttcga gatgataata ggctaatcat tatcgcaaca cataatccag caatttggga
    17161 gatggctgat gaagtgttca cgatggatcg tctgaaataa aaatccttgt ttttaattgc
    17221 acgatgagtt actgaaatat tatcataaat caataattgg agttaattta gaattgtact
    17281 ttattaatat tgaggtaact ttttcttgat aaaggaagaa atagtggaga ggaagttaga
    17341 atgaaaaaat tcgacaatta tattgttgag aagccttgcg attctaattc agataaactg
    17401 caaaaaatct taataattga aaatttggta gatgatattt tgcaatttcc tctcagaatc
    17461 aataatattg taggagagat tttcctccta caaccgtttc aaaagaaaac tatctttatt
    17521 ccatgttatt ttgaggaaga tattgtgaaa gtcaaagatg atgataaagt tgagtggaat
    17581 ttgttagaat ttcaaaaatt tagagcattt ttggcttagt aatctgtgtt gaaggctcaa
    17641 aacctatggt aaaaaagtcg ctttgaaaac gtattgcctc caaagattta gttaaataat
    17701 gatttaacac aaaaagaaat tattgaagtt ctggaaagat gttgtttcag tattgagaaa
    17761 aggtgggaaa aacttgcgat tttcacagag aaaggaagaa aaagtataga aatatagtca
    17821 attgaaacaa gaacaggaca aaagaacctt ttgtgccata tttttctcct ttcgctttac
    17881 aattggcttg aacaccttta ttgtatcgcg tttggagttt ttttggtata accttcgacg
    17941 cgcacccgca tagcgggtgt ttttttgtct cgcacctaac ggagcgagac gaactaatag
    18001 tcacttaatc aaaaaacgca ccatatcaaa aactaaaaag tttgatatca tgcgtcatgt
    18061 cttaaactaa ttgactatac tttctattca aatgagcttt taaccaattg attgagccaa
    18121 tccactctta aaaccaaaga gcaatttctc gcttagctga ctcttctgaa tctgaaccat
    18181 gtacaacatt ttggataacc tcattttctc cagcagcttt tgcaaaatca cctcgaatag
    18241 tgcctggtaa agcttcttct ggacgagttg cacccatcat ggtccgccaa gtttcgatta
    18301 ctttgggacc agaaatgaca cccacaagaa ctggacctga agtcatgaat tcacgaatcg
    18361 gtgggtaaaa actctgacca accaagtcct gatagtgctg gtcaatcaac tcttctgaaa
    18421 cttgtgaacg aaactccaat ttttcgattg taaatccacg ttgttcgatg cgctttaaca
    18481 cttcacccac tagccctctt tttacaccat ctggtttgat gataaagaat gtttgttcca
    18541 tacccgtctc ctttgtcagc ttctttcttt tattttacca catttcgtgg aaaaatggag
    18601 aaagttttca gaatagagaa tgagagaacc cgagggttct ctcattctct attattctac
    18661 tgtttcttcc acagtttcaa cggcagtatc cacaactact tctgttgttt cttcatttcc
    18721 ttcttcctct actggaggat taaggtattc ttcttcgttg acagcatatg gttcaaggtt
    18781 acggtaacgg gccataccag taccagctgg gatgatctta ccgatgataa cattttcttt
    18841 aagtccaagg agatggtctt tcttaccacg gatagctgcg tcagtaagga cacgagttgt
    18901 ttcctggaag gaagccgctg acaagaaact gtttgtttca agtgaggctt tggtaattcc
    18961 cataaggact gggcgacctg tcgctggaac tccacctgcg ataaggacat ctttgttggc
    19021 atctgtaaag tcattgatat ccatgagggt acccatgaga agatctgtat cacctggatc
    19081 catgacacgg actttacgga tcatttgacg aaccattacc tcgatgtgtt tgtcaccgat
    19141 ttctacccct tggctacggt aaactttttg tacttcaccg agaaggtacg tttcaactga
    19201 caagacatca cgaactgcaa ggagacgttt tggttggata gaaccttctg tcagagcagc
    19261 accacgcgct acttggcccc caacttcgac acgcatacga gctgtaaatg gaacgacata
    19321 ttcaccttcg ccagtttcac ccttaacaaa gactttcttg gtacgagttg atgcatcttc
    19381 ttcgatagca gtaacttctc ctttaacctc tgtgataacc gcttcccctt taggattgcg
    19441 ggcttcaaag atttcttgga cacgaggaag accctgagtg atatcggtat ttgaggcaac
    19501 cccacctgtg tggaaggtac gcattgtaag ctgtgtacca ggttccccga tagattgggc
    19561 agcaatggta ccaactgctt caccaacttc aaccgcatca ccagtcgcca agttgatacc
    19621 gtaacagtga cggcagacac cgtgacgagt gttacatgta aatacagaac ggatagtcac
    19681 ttcttccaca ccagcattga caatttcacg cgccttgtct tctgtaatca attcatttgg
    19741 accaataatc actgcaccag tttctggatg tttaacagtt ttcttagtgt aacgaccgtt
    19801 gagacgctct tcgagagact cgatcatctc ttttccttct gcgatagaac ggatcaagag
    19861 accacggtca gttccacagt cgtcctcacg gatgataacg tcttgggcaa cgtcgaccaa
    19921 acgacgagtc aagtaacctg agtcggctgt cttaagggcc gtatcggtca tacctttacg
    19981 agcaccgtga gttgagaaga acatttccaa taccgacaaa ccttcgcgga agtttgaaag
    20041 gattggcaat tccatgatac gtccattcgg agcagccatc agaccacgca taccggcaag
    20101 ctgtgagaag tttgagatgt taccacgggc tccagagtcc atcatcataa cgattgggtt
    20161 cttaggatct tggttagcaa tcaagcgttt ctcaagtttt tcacgggcag cacgccattc
    20221 agctgtaaca gcattgtaac gctcgtcgtc tgtgatcata ccacgacgga attgtttggt
    20281 gatttgttcg acacgtttgt gtgattcttc aatgatttca gtcttgtcat caacgactgg
    20341 gatatcggca atacccactg tcaatcctgc aagagttgag tggtggtaac cgaggttctt
    20401 catgcggtca agtagggcag aagtttctgt cgtacggaaa cgtttgaaga tttcagcgat
    20461 gatatttcca aggtttttct tcttgaatgg agggttgagc tcaagattgc tgatagcttc
    20521 cttgatatct ccaccaagtg gcaagaagta tttagctgga acaccttctg tcaagttggc
    20581 attgtttggt tcttgcaagt atggtagccc ctctggcatg atatcgttga agagaatttt
    20641 accaactgtt gtaagcaaga ccttatgtct ttgctcttct gtccaaggct tgttgaggct
    20701 gtctgttgcg ataccaacac gtgagtggag gtgaacataa ccattgcggt aagccataac
    20761 cgcttcgtca cggtctttga agaccattcc ttcaccttcg cgaccagctt cttccatggt
    20821 caagtagtag ttacccaaaa ccatgtcctg agatggagta actaccggtt tcccatcttt
    20881 cgggttcaag atgtgctcag cagctagcat gaggatacga gcttctgctt gtgcttcttc
    20941 tgaaagtggt acgtggatgg ccatttggtc cccgtcaaag tcggcattgt aggcttcaca
    21001 gacaagtggg tgcaagcgaa gagccttacc atcaattaag actggctcga aggcttggat
    21061 acccaaacgg tgaagggtcg gtgcgcggtt caaaagcact gggtgttctt taatcacttc
    21121 ttcaaggata tcccagatac gctcatctcc gcgttccacc aagcgtttag ctgctttgac
    21181 gttttgcacg atatcacggg caacgatttc acgcatgaca aatggtttaa agagttcaat
    21241 cgccatttca cgtggcacac cacattggta catcttaaga gttggaccaa cggcgataac
    21301 ggaacgtcct gagaagtcaa cacgtttacc gagcaagttt tgacggaagc gtccttgttt
    21361 acctttaagc atgtggctca atgatttcaa tggacggcta cctggtcctg tgattggacg
    21421 accacgacga ccattgtcaa tcaaagcgtc aactgcttct tgaagcatac gtttctcatt
    21481 ttgaacgatg atacctggtg catttaactc aagcaaacga gccaaacggt tgttacggtt
    21541 gataacacgg cggtaaaggt cattcaagtc agatgaggca aaacggccac catccaactg
    21601 caacattgga cgaagatctg gtgggataac cggaaggatg ttaagaatca tccattcagg
    21661 tttgtttcca gacttgtaaa aggcatccaa aacatccaaa cgacggatgg ctttgacacg
    21721 tttttgtcca gtagctgttt tcaattcttc tttgagttca gcaatttctt tttcaagatc
    21781 tacttgcttc aaaaggtctt ggatggcttc cgcacccatc ttggcaacaa atgaaccata
    21841 accatattcg cgcaagcgct ctcggtattc gcgctctgtc atgatagact tgtgctcaag
    21901 tggtgtatcc ttaggatcaa tcaccacata agccgcaaag tagataactt cctcgagggc
    21961 acgagggctc atatcaaggg tcaagcccat acggcttgga atccccttga agtaccagat
    22021 gtgagataca ggagctttca attcgatatg tcccatacgc tcacgacgaa ctttcgtacg
    22081 cgttacttca accccacagc ggtcacaaac aattcctctg taacgaatgc gtttgtactt
    22141 accacaagca cattcccagt cttttgtagg accaaagatc acttcatcaa agagtccttc
    22201 acgttctggt ttcaaggtac gataattgat tgtttcaggt tttttgactt ctccataaga
    22261 ccatgaacgg actttacttg gagaagctag ggtgatttgc atacttttaa aacgatttac
    22321 atcaaccact atttcttccc tttctattct aagtgaactg cttattcctg ttcagcagct
    22381 tcttctgttg cttccgcttt tgttgctttc tcagcttctt cagcttcaaa ggctgcttta
    22441 gcctcttggg ctgctttttc gcgggctttt tcaaggtcat ctacgtggat gacatcttcg
    22501 tccattcctt catccaagtc gcgaagttcc acttcttggt catcttcgtc taggacacgc
    22561 atgtcaagac caagagattg caattctttg acaagaactc ggaaggattc tggaacacct
    22621 ggttttggaa ttggtttgcc ttttgtaata gcttcatagg ctttcaaacg tccgttgata
    22681 tcgtcagact tgtaagtcaa gatttcttga aggacatttg acgcaccgta ggcttcaaga
    22741 gcccaaacct ccatctcacc gaaacgttgt ccaccaaact gagctttacc tccgagtggt
    22801 tgttgggtaa cagttgagta aggtccgact gaacgcgcat gcaatttatc gtcaaccatg
    22861 tggtggagtt tgatcatgta catgactcca acagaaacac ggttatcaaa tggttcacct
    22921 gtacgtccat cgtaaaggat tgtcttggca tcgctatcca tacctgcttc tttaacagtt
    22981 gaccaaagat cttcagaact tgctccatca aagactggtg tcgcaatgtg aataccaaga
    23041 gtacgagctg ccataccaag gtgaagctcc ataacctgac cgatattcat acgtgatggc
    23101 accccaagtg ggttcaacat gatgtcgact ggagttccgt ctggaaggta aggcatgtct
    23161 tctacaggaa cgatacgaga gacaacccct ttgtttccgt gacgtccggc cattttatct
    23221 ccgaccttaa tcttacgttt ttgagcgatg taaacacgaa ccaacatgtt aacacctgat
    23281 tgcaactcat ctccatttac acgtgtaaag atcttaacat cacgaacgac accatcggca
    23341 ccgtgtggta cacgaagaga agtatcacgc acttcacgag acttgtctcc aaagatagcg
    23401 tgcaagagac gttcttcagc tgaaagatct ttctcaccct taggtgttac tttacctaca
    23461 agaatatcac cttctttaac ctcagcacca atacggataa tccccatttc gtcaaggtct
    23521 ttgagggcat cttcaccaac gtttggaatt tcgcgagtga tttcttcagg cccaagcttt
    23581 gtatcgcgcg tttctgattc gtattcttca aggtgaacag atgtgtagac atcgtccttc
    23641 accaagcgtt cgctcatgat aacggcatcc tcgaagttgt agccttccca agtcatgtag
    23701 gcaacgattg ggttttgtcc aagcgccatt tctccatttt ccatagaagg tccgtcagcg
    23761 atgaaatcgc ctttttcaac gacatcacca acttttacaa gagtgcgttg gttgtaagca
    23821 gtacctgagt ttgaacgacg gaatttttgg atgtggtaaa catccaatga accatcttca
    23881 cgacgaactt ctaccttgtc agcatctgcg taagtaactt taccatcata ctgagcaatc
    23941 acagccgcac cagaatcgtg ggctgcttgg tattccatac cagtaccaac gtaaggtgcc
    24001 tgaggattaa tcaatggcac agcctgacgt tgcatattgg ctcccatgag ggcacggttg
    24061 gagtcatcgt tttccaagaa aggaatacat gctgtcgcaa cggcaactac ctgttttggt
    24121 gaaacgtcca tgtagtcaac aatattagct ggatactctt ggttgacccc ttggtgacgt
    24181 cccatgacaa tcttctcagc aaaggttcca tcttctttca gacgagagtt agcctgagct
    24241 acagtatatt catcttcttc atcagctgtc aaccaaacaa tttcgttcgt gacaacacct
    24301 gtttcacggt caaccttacg gtatggtgtt tgaacaaaac catatttgtt caagtgtccg
    24361 taagatgaca agttattgat caaaccgatg ttaggtcctt caggtgtctc gattggacac
    24421 atacgaccat agtgagtgta gtgcacgtca cgtacttcat atccggcacg gtcacgagtc
    24481 aaaccaccag gtcctaaggc tgacaaacgg cgtttgtgag acaactcaga aagcgggttg
    24541 tgttggtcca tgaactgtga caactgtgat gaaccaaaga attctttaac tgcagctgtt
    24601 acaggacgga tattgataat ttgttgtggt gtcaagactt cattgtcctg aacagacata
    24661 cgttcacgga cattacgttc catacgagaa agtcccaaac gtacttggtt ggcaagcaat
    24721 tcaccaaccg cacggatacg acgatttcca aggtggtcga tatcatctac acggccaagt
    24781 ccttcagcca agttgaggaa gtagctcatc tcagcaagga tatctgcagg agtcaccgta
    24841 cgaaccttgt catctgggtt agcattacca atgatcgtta cgacgcgatc tggatcagtt
    24901 ggagcaacaa ccttgaattt ttgaagaaca acaggctcag tcacaacggc tgcatcgttt
    24961 gggatgtaga caatcttgtt caagtcgcca tccaaatggc tttcaatgct ttcaatcacg
    25021 ctacgagtca taatcgtacc agcttctacc aagatttctc cagtttcagg gtctaccaat
    25081 ggctctgcaa tggtttggtt gagcaaacgt gttttaacat tgagtttttt attgattttg
    25141 taacgaccaa ctgctgccaa gtcatagcga cgtgggtcaa agaagcgagc cacaagcaag
    25201 ctacgtgagc tttcagctgt cttaggctca cctggacgaa ggcgttcgta aatttctttc
    25261 aaggcttcgt ctgtacgaga gtccattgga ttcttgtgga tatctttttc aacagtgttg
    25321 cgaaccaatt cgctgtcacc aaagatatca aagatttcat catcacctga gaaaccaaga
    25381 gcacgaacca aggttgtaaa tggaatctta cgagtacggt cgatacgagt gtaggtgata
    25441 tcttttgagt cgctttcaag ttccaaccaa gctccacggt tagggataac agttgaacca
    25501 tagcccacct taccattttt gtctactttg tcgttaaagt aaacacctgg tgagcggacc
    25561 aactgagaaa cgataatacg ttcaccacca ttgatgatga aagtacccat ttctgtcatg
    25621 attgggaaat caccaaagaa aacttcttgg gtcttgattt cgcctgtttc tttattgatc
    25681 aagcggaagg ttacaaaaat tggtgctgag tagctagcat cgtggatacg agcttcttct
    25741 agcgtgtatt ttggttcctt gatttcatat ccaacaaatt ccaactccat tgtgtctgtg
    25801 aagtttgaaa ttggcaatac atcttcaaac acttccttaa gaccgtggtc taggaaagct
    25861 ttgaatgagt cagtttgaat ttcaatcaaa tttggtaagt caagaacttc tttgattctt
    25921 gaaaaactac gacgggtacg atgtttcccg tattgaacgt catgtcctgc caagatgatt
    25981 ctcctttgta aataagttcc aagccttgtc aatcaggctt ttctaatcgc catatggttg
    26041 taaacccctt atcaccgtgt cctcttgacg aattttcaga atctttaagc ctctgttaca
    26101 aatgctcaaa atcttgaaaa aaagcacaaa aagagcagct aaatctgact ttttcagaag
    26161 atttaactgc tgtgagcctt gtctggacaa tatttcagac aaaacctacg acaaatgatt
    26221 acccatatta taccctattt agctagattt ttcaagggga ttcagtaggt ttttggtaat
    26281 tttttcccat agaaaacttg gcatcacatt cgaatcacgc tatggtacaa aaaactgaaa
    26341 aaactattga ctgaaaatca ttttcaaggt ataataataa acgttaaggc ggtatagcca
    26401 agtggtaagg cacggctctg caaaagcttg atcgtcggtt caaatccgtc taccgccttc
    26461 tataacttga tttatcaggt ttcaaatgaa cagaaagccc aatttgaagg gcttttttta
    26521 ttttccctcg aataaatacg tataacttta aaaacttttg gagcgagttt gtggcagagt
    26581 tctttccatg gcataattcc cttttgaaat caattgaaaa aagcttcgtc acgcctggtg
    26641 acaaaagcct ctagtttatt ctgtttcttc ttctatttcg acttctgtga tttggacttt
    26701 taccttggta acacgtccat tcttcacttt atcattggtt aagataaact gtttgttttg
    26761 gctgaccaat tcatagctga gtttctcagt cgttggaatg gttcccactc ctgtcaaata
    26821 ataaccagcg atagtatcaa cgtcatcact ttccagttca acattaaagt aattattgaa
    26881 atcattgagg gtcatagttc cttgaacaat gtaggtatcc tcgccaatct gatgaacatc
    26941 tattgctgct ttgtccgttt catcatcaat ttcaccaaca atttcctcta agaggtcttc
    27001 caaagtcacc aaaccagcca taccaccgta ttcatccagc aagattgcca tttgtctttg
    27061 ggtatttcgc agttctttta gcaagtcatc cacaaaaata gtttcaggta caaaaagtgg
    27121 atcttgtaaa attctcttcc aaacaatatt gtcaaaaccg tccacaaagc ctgccttaag
    27181 gagactcttg gtgtgaatga ttccaattac attgtcctta tccccatcat aaaccgggat
    27241 acgagaataa ttttgtttta aaatactttg gataatggct tgactatcat cctgaatatc
    27301 caccataaag gcatccgttc gaggaaccat aacctctcgt gccatcagtt catcgagcga
    27361 aaagacacct tgtaacatct caatctcatc agcatccaat gtttcttcac tatttgtcag
    27421 catataggca atttcatcac gggtcatctt ttcatccgca tcatcgaatg acataggagt
    27481 caaatggctc aagaaattgg tcgaagcagc taaaagccaa acaaaaggac tgactagttt
    27541 tccgatccca atgataaccg gcgctgtacg aattgccaag gcatccttta gattaagagc
    27601 gattctctta ggatataatt ccccaaaaac gatggaaata taggtcaaaa atgccaagga
    27661 tagaaaagtt gccacggctt gtgctgtttc gccattccca agccaagagg caatcacacg
    27721 tcctagagta tcagttaaac tcgcccctga taagattgta atcagggtga ttcctacctg
    27781 aatggttgat aaaaagtggt taggattttc tagtaccttc agcaggcgga tgtagcgtct
    27841 gtctccttct tccgcctttt gttcaactcg ggcacgatta agagaaacca tggccatttc
    27901 tgtggctgag aaaaaggcat ttaataccgt caagataaac aataaaacaa attgtagcaa
    27961 caaattctga ctacttgggt cttccatagt tcttctccta aaatagtatc ttatccttta
    28021 ttatatcaca aaatttaatc caatacatat tatttttttc ttagcgtaac ttccatctct
    28081 ctcccaaaac tggaagttag tctaagacgg atttccactg atttcacgcg tttttctcat
    28141 actaaaagct aggttgtggg aaactggaag ttagttttag aagttactat tttttcttag
    28201 tacctgttct agccctgctt aaactagtaa aaagaactcc tacatccata agtgacatag
    28261 gagtttaggc ttatctttta ctgagttaca gttacttctc cagttcggta atccagttga
    28321 agtccctttt gaacattggg aactagaaca ttgaattcca attctccatc cgaagacttg
    28381 gcttcaatct gtgaagctga gggaactaaa tcctcgtttg aagcgtagta aagggttaca
    28441 cggtaacgga cacgcttgtt ttggtccaag gctttacgca ccttgctttc atagtagttt
    28501 tgaccagtcg aatactcggc ttgtgcctga tttgcccagg ctgtctgaac agcaatgttt
    28561 ttaggattgc ttgttgaggc atcaaaacca tccaaaccac cgattaaggc atagcctaac
    28621 aaatgacctc tatcgactgc atgggtataa gagcccttta gattcttgac ctgatgccaa
    28681 cctggaggag tccaagaagt tgaaccattc ccagtttctt tacgattctt gtactgacga
    28741 gtggccttag acaagagggc attagctacg gttggaacag tttccttacc cactgtcttt
    28801 gttttattgt cagcgtaggg cttacttgaa accttggcat ctagatttgt tttattacca
    28861 ttgacgataa aagcacctga gccattccac tccagactcc cctttatttg actcttgact
    28921 gcgtctgtta agacactctc tgccaatgct tgactaggag cttcaggcgc ttgttttttc
    28981 tgactaagct tggttttagg actattagct gtcgactgca tctgcttgat ataatagctc
    29041 cctgtagaca aaagcaataa cactagcagt ccgattagtg tctgtcttgt ttttttgttc
    29101 atatttctcc ttatctttag aaaaaggctg gttctccagc ctaatttcct gtaaatttat
    29161 gaatcaattc ctgccatttt gctggagaca ggatagccca tgaatcttga cccttgccca
    29221 agattccata acctaccatt aaaccgattc ctagagccag agtacctaaa atcagtacta
    29281 tgatgactaa aagtaaacgc ttgactacgt agcttgattt cttattcatc ttcatcactt
    29341 gcctcaatcc gctgaatctt agcacctagc tgcgccaact tctcatggaa accgtagtaa
    29401 cctctatcca agtgaaccaa tttaccgacc acagtttctc cctgtgctac caaacctgtc
    29461 aaaatcaaag ctgcactggc acgaaggtca gttgaaagaa cttctgctcc ctgcaaaggc
    29521 tgtccaccaa caatacgagc tgtatcacgg ataatctcag aatgcaagcc catgcggcgc
    29581 atctcttcta ggtgttggaa acgattttca aaaactgtct ccaccatggt tgattcgcct
    29641 tttgcaactg tcatcaaggc tgtaaattga gcctgcatat ctgttggaaa tcctgggtgg
    29701 ggcaaggttt tcacatgaac agcttttaga ttttccagtt gagaacgaac acgaattcct
    29761 tcgtcttctt caattacttc aacacccatt tcaagtaact tggcaatcaa gggacggttg
    29821 tgctcccaga cagcgtctcg aatcaagaca tcaccaccag tcatggcagc agctaccata
    29881 aaggttcctg cttcgatacg gtcctggact acattgtgag tcgtaccatg aagtttctca
    29941 acaccagtaa tggttatagt ctctgtacca gcacctttga ccttggctcc catttcatta
    30001 aggagaatgg ctaagtcaac aatctcaggc tcacgcgcag cattctcaat cactgtcacc
    30061 ccatcagcca gagtcgctgc catcatcaag ttctgcgttg caccaacact tggaaagtcc
    30121 atatagatat gagcaccatg caagcgttct gcctttgctt cgatgtaacc agctgtctga
    30181 ctaatcttaa cccccatagc ttccagacct ttcaaatgaa gatcaatagg acggctacca
    30241 atcgtacaac cacctggcat ggataccttg gcatgaccca cacgggcaag gattggccct
    30301 aatacaacga tggaggcgcg catcttactg acatacttgt aaggggcttc ctcagtgatg
    30361 tcgccagtag catccacctt gacaagatga gcttcctcat caaagtcaac cttggcattc
    30421 aaaccaccaa ctacctgatt cataataaag acatccgaca aaatcggaac attctgcaag
    30481 acggtctttc cttcacttgc tagaatagtc gctgccaaca agggtaagac tgcatttttt
    30541 gctccctcga tcgtcacgct tcctaccaga cgattatcgc caccttgaac cacaatttta
    30601 tccatactcg tttcctttac tcttgatttt ataataatcc tctaagcgct tcttgccaca
    30661 aatgatacag gctaataaag aaagaactca ggatgtagcc tatgacaatg ctaaaaaagg
    30721 ctactaatac acgaactttt cttgtattct cagctgtcac ttttaaaacc ttttcccatc
    30781 taacaagatc ctttaaaagg taaaaactca cataaataaa gagcatatga ctacttagag
    30841 tgaataataa ttgaaccatt tgactattat accatcttct ctgtttgtgc gctagtttgg
    30901 aaacttcact caaaaattag ggattgtttt tcaagtaatc aatcgcatct tgtagggttt
    30961 taacgggcac gattttcata tccgtcttga ttgttttagc cgcttctagg gctgtttggt
    31021 agttgttttt cgcgtccgga tgcgcctttt gttcttcttc gctaacaggg ttatcaggag
    31081 caaagaaaat agcagcacct gccctagccg aagctacaac cttcttatca atacctccaa
    31141 tgtctcccac attaccatcg cggtcaatgg tacctgtacc ggcaacaata cgaccattac
    31201 gaagatctgg gtgagctatt tgagtataga tagctagact aaacatgaga ccagcacttg
    31261 ggccgccaat accagctgtt gaaaagctaa ttgggacatt gctgattacc tctgtacgat
    31321 caatcaagcc gattccaatc ccattttttc cattttccaa ggtgataatc ttcccttctg
    31381 cagacttggt ttgcccatcc tcttcatagg tgaccttgac ggaatcccct aatttttgag
    31441 aactgacgta atcaatcaag tctttggaac tatcaaaggt ctgatcattg actgctgtga
    31501 ctgtatcaga gatattgaga atccctttaa aggttgaatt atccgtcaca ttcaaaacat
    31561 aaactccaaa gtacttgagt tcgatatcct taccagctgt ttttagtcct tgatacttgg
    31621 ccatattttg cgatgtttgc atgtagaatt gattgattcg cataaattca acatcggaag
    31681 aaccacctgt agtctcctga gcactacgaa tatctgtaaa aggtgtcaac caagcataaa
    31741 tcatatgagc taaagtagca tgttgaacac caaccgtaac gaattgatag gcaccagctt
    31801 ccttatcttc tgtgtcattt actttaagga cttggcgaat atcttccgaa ccacctggaa
    31861 cctctatata ataaggcaag ggcactacaa atgccaagaa agtcacaatc aaggccgcaa
    31921 tgacgtataa gggccatcta atcttttttt tcatttctta tttcctccaa aatactctcg
    31981 ggaacatagc aggcaatatc ctgaccaaac ttcaaaagct ctctaacgcc tgatgaactg
    32041 atatagagat gttcaggtcg actatgtaaa taaatagtct ctatatcaga agacagctga
    32101 tgattgtagt aatcaaaact ggcttcatat tgcaaatccg acgcattcct caaaccacgc
    32161 actagacaag tagcacccaa tctttttgca acatcgacca ccaattcatc atgagaagcc
    32221 acgacttcaa cattttccaa atgtcccaaa gccttttcta gcccccgttt acgattttca
    32281 agagggagaa atccttgttt gtggggatta aaaaaaatac ccacacaaag cttatcaaaa
    32341 agtctgctcg cccgttcaat gatatccaga tgcccatttg tcatcggatc aaatgagcct
    32401 gtgaataagc caatcttatc tgacatagac tgtcacctta ctaattccat aaatcttttc
    32461 cttccagata cccagacagg caatttcttc tggaagttca acggtcttat ccgtctcgca
    32521 cacaaccata acatcttcag aaaaaagctc tctctcagcc attttttcaa tatctgctac
    32581 gatttgttcc ttggcatagg gagggtctaa gaaaacgagg tcaaattccc cagatacctg
    32641 ttccaatgcc ctttctgcat ccattttgag gagttgaaat tttccaactt ccttggtcat
    32701 ctggatattt tcagccacga tggtctgagc cttacggtct cgctccacca aaacagcact
    32761 ggacatgcca cgcgatactg cttcgataga taaaccacca ctacctgcat aaaggtccaa
    32821 gactcgtccc acttcaaagt agggaccaat catgttaaaa atggctcccc taaccttatc
    32881 cgaagtaggt cttgttgtct tgccttctag tgtcttgagg ggacgtcccc catagattcc
    32941 tgatacgatt ttcatactgt ttattatacc aaattataga caaaaagaga aagaaaaccg
    33001 aaccttgcgg ttcgattctc tacaaaatat tttcgtaagt atcgcggact tcttgaggcc
    33061 aaacacttgt ttgcacttct ccgatgtgtc tcttgcgaag taggaacatg gccatacgag
    33121 attgtccaat tcctccaccg attgtcaatg ggaataggcc attcaacaaa gacttgtgcc
    33181 attccaattc taagcggtct tcatcacctg taatttccac ctgacgtcta agagtttctt
    33241 catctacacg aattcccata gaagacaact caaaggctcc acctaaagac tcattccaga
    33301 caagaatatc accatttaga cccttgtagc cattctcaga ctcgcttgtc cagtcatcat
    33361 agtctggtgc acgtccatcg tgcggtttac catctggcaa ctcgccaccg ataccaatca
    33421 aaaagacggc tccaaattct ttacaaatcg cattttcacg ttctttaggt gtcaagtctg
    33481 ggtagcgttc taccaattct tctgtatgga taaaggtgat ttgttttggc aagatagact
    33541 cgatgtcata gcgggcttca acagctagct cagtcaggcg aatagcctta taaatcttct
    33601 caactgtttc ttttagataa acgatgttac gcttaccatt tgggataacc ttctcccagt
    33661 cccactggtc aacataaaca gagtgggttg catccaagga atcctcatct ggacgaaggg
    33721 ctttcatgtg gacaaagaga ccctctcctt caccaaagcc aaaacgagcc aaggtgtggc
    33781 gtttccattt agcaagtgag tgcaccactt cataagtagc atcagggatt tggagaacct
    33841 tgaccgatac tggattttcc acaccagaca ggttgtcctg cattccgtca ccgaccttac
    33901 tcaagatagg accttgaact tcgacaactt ctagcttatc tttcaaatac tgggtaaaag
    33961 tgtttttgac aaaggaaatt tcttcttgtt gatggataaa acttttcttc atagacaact
    34021 cctcaaaaat ttaataaacg tattatacac ctatttccaa gaaaagaaaa gagaaaattt
    34081 taaaaaaatc agtgtcactc aaaacactga tttcatagac cttttagtca ttgcgaccaa
    34141 agatacgtag aatacttagg aaaagattga taaaatcaag atagatgctg agagccattg
    34201 acaccaccca gcctgtcgct acacgacctt gtgattgttc ataggcgagg cgaatctttt
    34261 ggttatccca agcaatcaac cctgaaaaaa ccaaaaccat ggctacgcta atcatataat
    34321 caaagaagcc actagccaag aaaatattga ctaccatagc aatcagtaaa ccaatgagag
    34381 ctgccatcat tgctcgacca atcccactca agtctttctt ggtaaaaata ccaactgccg
    34441 ccatgacaaa gaagagaagg gcgctcgata caaaggcaga taaaactgta cccggagtat
    34501 agaaagccac cacaaaacta agggtaaagc cgtttaaaac ggagtaaagt aaaaatactg
    34561 gaagagcagc tggactattt cttgaagcca tgctactggc aacgaaaacc aaagccaact
    34621 ccgcaaaagt agcaatggtc aaccagagac gcccctgcat caacaagtaa accaactgag
    34681 actgaaagac cgtcaacata aggcctgata ccaaagcaga tagtccgatt cccagaccaa
    34741 caaaggcata aaccttagcg taaaattgat tgagacctgc acggtcatgt ataatagtgt
    34801 gattcatcct tttctccttt tctgtgaaca tctttccttg attataacaa aaaaagttaa
    34861 aattagctta aatggaagat taaaataccg gctgcaactg caacattcaa actctcggcc
    34921 tgccccttca tgctaatatg gaccaactgg tctgcacttt cagccataag gggactaatt
    34981 ccttgaccct catttcccat aactagtaca aaattttcta tagaaggcag ttctctgtaa
    35041 tcaacagaat ctttagatag ggttgttgct agcactggga tagctacctt tttagtttcg
    35101 tcaagaagcg cttgactagt catccggtaa atgggcagat ggaaatgact accttgcatg
    35161 gaacgtaggg ttttgagact gtagatgtct gccgacttat ctgaaacaat cactcctgta
    35221 aaacctgctg catccgcagt tcgaatgata gttcctacat taccaggatc ttgcacatct
    35281 tccaaaaaca agaacttgcc ctgactcaaa tcagcttgtc ctacttcttc tttttgaacc
    35341 acggcaacaa ttccctgtgg agtctgagaa tccgccaaat ctagcaaaat atcctctgaa
    35401 atccagacag tttgcggaaa agcagctaac tgatttcgat aattttctag ggcaaagatt
    35461 ttctcaatcg tcactccagc ttggacagct tcttcaaaca agtgccagcc ttcaatcaaa
    35521 taggcagact tgcggtattt tttttggtgt aatttcttgg cattttttac cacagaattg
    35581 gcttttgagg ttataatagt catagaaata ttataacaca atcaaagggg tttggtatgc
    35641 aaaaggttag aatgattgcc caaggtaggg tgcagggagt cggctttcgt tggggtgttt
    35701 atagcctggc ccttgaaatc ggtggcatca caggtcgggt atggaataac gacgatggca
    35761 cagtggaaat cttagcccaa gcagactcat ctgctatcat ggcaaaattt atccaagaaa
    35821 tccgaaaagg accgacacct ttttcaaaag taagctactt agatgtcaaa ctaagcaact
    35881 ttcctcccta ctctgacttt aaaatcgcaa attaggtctc cagaactatt gtatattttg
    35941 taaaaaaaca gtagaataga aaggtataat ttttaaagaa ggaataaaaa cagtgaaatc
    36001 tattaaacgt tttgcattct cggctatggg agtggctatg ttgcttgtct tgactggctg
    36061 tgtcaatgtc gataaaacca caggtcagcc aacaggattt atttggaata cgatcggagc
    36121 gcctatggct gaagccatca agtacttcgc tactgataaa ggtctaggct ttggtgtcgc
    36181 tatcattatc gtaaccatta tcgtacgctt gattatctta ccacttggta tctaccaatc
    36241 atggaaggca acgcttcact ctgaaaagat gaacgctctc aagcatgtcc ttgagccaca
    36301 ccaaacacgt ctcaaagaag caactactca agaagaaaaa ctcgaagccc aacaagccct
    36361 ctttgccgct caaaaagagc acggcatcag catgtttggt ggtgtaggat gtttccctat
    36421 cctccttcaa atgcctttct tctctgctat ctactttgct gcccaacata ctgaaggggt
    36481 tgctcaagca agctacctag gcattcctct aggttctcca agtatgattt tggttgcctg
    36541 tgctggtgtc ctttactatc ttcaatcgct cctttcactt cacggagtag aagatgaaat
    36601 gcaaagagaa caaatcaaga aaatgattta catgagccca ctcatgatcg tcgtcttctc
    36661 cctcttctca ccagctagtg tcacacttta ctgggttgtc ggtggtttca tgatgattct
    36721 ccaacagttt atcgtcaact atatcgttcg tccaaaactt cgcaaaaaag tccgtgaaga
    36781 actagccaag aacccaccaa aagcaagtgc tttctctaaa ccaagtggac gaaaagacgt
    36841 tacccctgaa caaccaactg ctatcacaag caagaaaaaa cacaaaaatc gcaacgctgg
    36901 aaaacaacgt tcgagataag aagaaaaagg cttagctgat ttgctaagtc tttttgcaaa
    36961 tcaaaagccc gatgtttcca tcaggcttct tttctatcca tgtacacgtt tcatataatc
    37021 ttgataactt tcggtatcca tgagtttttt agcgttcttg acgcgatctg ctgttggtgg
    37081 tttgactcct tcgagggaat atggaattcc aagttcacgc cacttgaact cacccatggt
    37141 gtgataaggt agaatttcaa acttatcaac atttttgagg gtcttgacga acttaccaag
    37201 ttcaatcaag tcatcatctc tgtctgtcaa tcctggaact agcacgtggc gaatccagac
    37261 aggttttcca atatctgata gatactgggc acaagccaag atatttttat tagtttggct
    37321 agtgacaatc ttgtgctgtt cttcgttgat ttccttgata tccaaaagaa ccaagtcagt
    37381 gacagccatg agtttgtcaa acttctcaag gtaacgtggt ttattacgga aaggaagagc
    37441 acaggtatcc aaggtacagt ggattccttg ttccttagcc ttggtgaaga gagcaatcag
    37501 gaaatcaatc tgcaagagag cttctcctcc actgactgta atcccaccct tatttcccca
    37561 gaaaccacgg tagcgcaagg cctctgtcaa gacatcatct accgtccgtt cacgtgactt
    37621 attggactcc atagcccaag tgtctgggtt gtggcaatac tggcaacgca tgtgacagcc
    37681 ctgcaaaaag acaataaagc gaataccagg cccatctact gacccaaagc tttctgtcga
    37741 atgcaccatt cctgtcactt gtccataatc aattgtttct tcagacatat cgttaccttc
    37801 cttgaaaacg ttttatagtt ttattatatc acgacttgaa ggaaaaacaa gatttttgcc
    37861 tattttttag aaagcaatct gtttttcaat ttcattttca atctattatc agattattct
    37921 tcaaaatcga agccatataa ggttgcaaaa tagtcctcag ggcgctctgc acggcggatt
    37981 tgacgggctt taccttcttc ggtatagagg atttccgcag aacgtaattt ggcattatac
    38041 tggtagccca ttgaaaatcc gtgggcacct gtatcatgaa ttaccagcaa atcaccgatt
    38101 tctgtatgag gcagttcgcg attgactgca aatttatcat tgttttcaca gagtgaacca
    38161 accacatcta ccgcttcagc tggtccatct ggatgggtca cgttgctaat atgatggtaa
    38221 gctccgtaca tagctggacg catgaggttg actgctgagg catccacacc tagataggta
    38281 cggtaggttt ccttcttatg agtcactctt gtgactagag caccgtgagg tgccagcata
    38341 aaacgaccca attcggtgaa aatcttgacc tgaccaagac ctgctgacgt aagaacttct
    38401 tcatacacct tacgaactcc ctcaccaatc aaggcgatat cgttcggctc ctggtctgga
    38461 cgataattaa caccaatacc gccagaaaga ttgataaagt ctagcgaaat gcccaacttt
    38521 tccttgattt caacagccag ttcaaagagc tgacgagcca actctggata atagagatgg
    38581 gtcacggtat tggacgctag gaaggagtga atcccaaaag tcttggctcc tttttccttc
    38641 aagatagcaa aggcttcaaa gagctggtcc ttggtcatgc caaacttagc ctccccagga
    38701 ttgtccataa tgtctgtccc cagttcaaaa acgcctccag gattataacg acaagagatg
    38761 atttctggaa tgcctgctac tctctccaga tgttcaatat cttcaaaggc atccaagtta
    38821 atggtcgcac ccaattcacg tgcataggcg tattccttgt ctggcgtgtt gttggaagag
    38881 aacataatct cagaacccag aaagtccagt ttatggctca tcaaaagctc tacataacta
    38941 gagcagtcca caccacaacc ttcttcttgg agaattttca aaatagctgg agttggagta
    39001 gccttaactg caaaatattc cttaaagccc ttgttccacg aaaaagcttg gttgacggct
    39061 cttgccttct cacgaatccc cttctcatca tacaagtgaa agggagtcgg gaactcggca
    39121 acaatcgctt ctaactcttc tctattgata aatggtgttt tcataagctt ccttctattc
    39181 tgtttgcaaa gaaaggctgg gacaatcgtt ccaaccttta gttctatctc ttatttatct
    39241 tcgtcaaaga tattgccaac ctcaacatcg atggcttggt tcttgacatc aatattggtt
    39301 accttcaaga gtgacttgta gtcaaactgc ttttcacgtt cttcttgggc attgtccgca
    39361 aagaccgcta cacctgccag ttctgagtcg aactcgtgca agagactaat cataccattg
    39421 accgttccgc cacctttcaa gaagtcatcc acaatcaaga cacggctgcc tgccttaaga
    39481 ctacgttttg aaaggaacat tttctcgata cggtcaccac ttgaacctga aacatagttg
    39541 acgctaacag ttgaaccttc ggtaattttc aggtcacggc gcacaatgac aaaagagaca
    39601 ttgaggacat tggcaactgc atttgcaagt ggcacaccct tagttgctac ggtcataacc
    39661 gcgtcaattt tttggtccat aaagcttttg gcaataatac gaccaatatt tttcaagatg
    39721 gctggtgtgc taagcaaatc agacagatag atataaccac ctggcaagat acggtcactt
    39781 tctgacaact tggtacgcaa gtcttcaacc atttccttag catcctgact cgaaatagac
    39841 ggtgtgaaga tgacacctcc gccagcccca gtcactgtct ggatatgacc gatttcaatt
    39901 tcctcaaagg cgcgtttgat aatgacgata tcttctgaga tggatgattt agcagactca
    39961 tacttttcag caaaagtatt gagactagtt agtttataag gattattaat caaatagttg
    40021 gaaatgacaa ccatccgatc acttcttctt aatttcattt ctatttccta ttccattttt
    40081 attcaaaaaa tcaaaaagca aactagaaag ctggtcgctg gtggttcaaa acactgtttt
    40141 gagattgtca atagaactga caaaccctgt aatatacctg catatataca tacgacaagg
    40201 cgatactacc ctagtttgaa gagattttcg aagagtattc atttttgtct tttacttatt
    40261 ataccatatt cacataaaaa aacgaacatt cttatcctaa aaaatgctca tttttcttaa
    40321 attatcaatc taaatctggt ttatagaagg aacgattatc catagcgaag attttattgg
    40381 tcatctctcc tttatccacc aaagccagag ctgttgacat catcatcatg cttgcatcca
    40441 gattgtcaat catatggata atctctgcct ccataatacg tggacggact ggacttccat
    40501 attcaagcaa gccgtggtga ctgaggatga catgacgaag caaaacgact tcttccttgg
    40561 tatcatcgat gccgagttcc ataactgtct tggtaatttc gctatcaatg agagcgatat
    40621 gtccaagaag attacctcgc actgtgtact ctgtctggtc tggccccgtc aactcgataa
    40681 ccttagctaa gtcatgcagc ataatccccg catagagcag gctcttattg agctgaggat
    40741 aaacttcgct aatagcgtct gccaaacgta ccatggtcgc cgtatgataa gccaaccccg
    40801 tttcaaaggc atggtggttg gtcttggcag ctggatagga gtagaattcc ttatcatact
    40861 tggtgtagag atttcggaca atccgttgcc agacaggatt ttcaattttg aaaatcattt
    40921 gcgacatgta gtcacgaatt tccttgacat caactggtga cttgaccttg aaatcggctg
    40981 ggtcattggg ttcaccagct tgaggcaggc ggagagtaat ttgattgact tgaggggtat
    41041 tgttataaac ttctcggcgt cctttcatgt ggacaacctt acctgcggta aaggcctcaa
    41101 tgttatgagg ttgggcatcc cagagcttcc catcaatctc gccactatca tcttggaagg
    41161 taaaggctag gtagtttttc ccagctcgag tttgcctcag gtcagctgat ttgattaggt
    41221 aaaagccttc aaatagctca tcttttttca tgtgactaat cttcatattc ttcctcattt
    41281 tcttgaaaat ggagtagatc aagcgcaggc tcaccttctg acaactcaat gtgacggagc
    41341 gtccgctcga tagctatggt acgacggttt aataattcat caatattgcc agaggcatgt
    41401 tggagatgtt tttgtgcctt gaccagaata ccaccaaact tgccaaactc ggtcttgaca
    41461 ctggcaagag tcttgctgat atggtcggca ctcttttgga tattaagggt cttgaaacca
    41521 actgatagag agttaagaag ggctgatagg gtacttggtc ctgcaacaat aatctgttct
    41581 tcccgtctca aatcatcaaa gaagaccgga ttgcgcacaa tctctgaata aagaccttct
    41641 gtcggaacaa acaagattcc aaagttggtt gtccgaggtg gtgctatgta cttgttccta
    41701 atatccctag caaagcgctt gacgcttgct aggagtgact tacgacagcg ttcaatctca
    41761 tccttgtcac ctgtctcata ggcttcttcc aagcggtaat aatctgccag tggaaactta
    41821 gagtcaattg gcagatagac gtattcttgg tcgccttgtc cgggtaactt gatggcatac
    41881 tccactcgtt cactagagtt ttcaaccgtt gcgtattctc gttcgtactg ggcaggtgtc
    41941 atgatgtctt caataatttg ccccagttgc aattctccca gaatccctcg cgtcttggtt
    42001 ccagagagaa ccttgttaag agctccgaca tcacgggcaa ctgtctgcat ttctccaagg
    42061 ccacgattga cagactccag ttgtttagaa actgtctcaa aggaagcctg taagcgtgtc
    42121 tgcaaggtct tttctagttt ttcctcgacc gtctggcgca tttgttccaa acgttgctca
    42181 tttgattcct gcaaggcttg gagacgttgg tctgtcttgt ctctagtttg gaggagatta
    42241 tctgtcattt cttgacggac ttgggtcaga ccttggtgca attcaatccg cacttcttgc
    42301 aaacggtcgc tgacaaccac ttccaaatct ttttggtcta actggctggc ttgtctggct
    42361 tggtcaaagc ggtaatccaa ctggtctgac aaatgatctg cctgatcctc caagctctta
    42421 cttaagtgtt tctcctgcct atcctgcctt tgccaaatca gaaagagacc agctagattg
    42481 gcaattaata atagaataag taaactttcc atcctacctc ctgtccttgc tatgcagtac
    42541 gaccacataa ccatctgggc aagttaccga cacttcccta tctatatatt cgttagaagc
    42601 gtacactttt ttaaagaaaa aattttcctc tgtcaactca tacttggctc caagaatagt
    42661 cagctggcta tcccgaactg gcataaaggc tagatagtcg tagtctgaac gaggttctag
    42721 ctgactgatt ccttctggac aataagtaat caagttttgc ccatcctcaa tttctatttg
    42781 atgcatatag gatgccaact taggattgct aggcagaaag acattggcca acatatggtc
    42841 aatacggcca cccaaggcac cgaaaatagt aacctgagcc tgaggatttt gcttaaagat
    42901 ggtcaagaga gccaattcca aatcagtatc atctttttct ggccgggctt ggacaaaata
    42961 ctgggcacgt ttttgaatca cctgtcgctc ttcttccgtc acagaatcaa aatctccgac
    43021 tgctagagca agaggcaaat cttcttccaa gatccagagc gagcctcgat ccacaccaac
    43081 aaaaccatca aagtccgtcc gataatgacc gcggtctccg cctgcaaaaa cggcaaccct
    43141 agtccagttg ttttctgaga gtttgtactc gctcattgac atctccctta aagacatagg
    43201 aacctgctac aaaaacggtc gcaccagctt ctttggcttg agcaatagtt tggtcatcaa
    43261 tcccaccatc cacttcaatc tcaaagttca aacctttttc atcacgaagg gcaaccagct
    43321 cacgaacctt atccatggtt tctggcagaa aggcttgacc accaaatcca gggttaactg
    43381 tcatgactag gacttggtca actagatgaa gcacatgctt aatcgcttca actggtgtac
    43441 caggattgat aacaactgat ggcttgacac cgagtgaacg aatcttttgg agggcaccat
    43501 gaatatgagg cgttgcttcc acatggatac taataatatc cgcacctgca cgcacaaagt
    43561 cctctaaatg atgctcaggg tttgacacca tcaagtggca gtcgaaaacc atcttactat
    43621 gaggacgaag agcttcgacc acacccgcac caaaactgat ttgcggcaca aaatgaccat
    43681 ccataatatc gatatgggca tactctgccc cagttgcttc taggcgttta atttcacgtt
    43741 caaagttggc ataatctgct gccagaattg acggagcaat cttgtattga gacataggtt
    43801 tctccttatt ttggaatttt tttgctgact tttttatagg tttctctgcg attttcaatt
    43861 tcgctgagga attgcaggta gttgtcaaaa cggaagggcg caatggtacc ttcttcaact
    43921 gctggcttga cagcacagga cggctcatgg gtatgggtac aagtacggaa tttgcagtct
    43981 cgactgacag tggcaatctc tggaaaggcc tgattgaggt cttcagccgt tgatacttca
    44041 taatccagtg atgaaaagcc tggtgtgtct gcgattttac ccccattgag gttgtaaaaa
    44101 ctaacagctc gagtggtatg acgcccgcga cccagactgt ctgaaatttc tcccgtttca
    44161 agattaagat ctggtgcgat tttattgaga agagttgatt tcccaacacc cgtctgcccc
    44221 ataaagaccg taaccttgcc tgttaacaaa ggcaggagtt cctctttact ggtcacaaag
    44281 tcatatccga tgtcaccata ggtctgctgg taaaaatcca gttctccccc gtcttccaac
    44341 aaatccattt tagaaatata gacgatggga tgaataccct tatgctccaa aagaaccaag
    44401 aaacgatcca gcaaattgct gttaaaatca ggttccttga cagacatgat tactacagct
    44461 tggtcgatat tgacaatagg cggacggacc agactgtttt tccgttcgtg aatcttgaga
    44521 atatagcctt ctgaattttc ctcggcagag aaatctaccc agtccccaac gtaaggggta
    44581 tgcccttttt tacggaaatt cccacgcgcg cgtgtttgat agacttggcc atcactctct
    44641 acatagtaga acccagccaa ggctttaatg atttgtccct gcaaaataac tcctttaaaa
    44701 ttgttagttc cattataaca aaaaaaagct attttagctt agcttctctt tacaatttcg
    44761 ataagtctat tctaaaagaa aaagatacac tcacgcttta gataccaagt tttatacaaa
    44821 aataacgaat ctacctcctt atataaaaac aggcgaacag agagcctaaa actccctgtt
    44881 cgctttactt tttctgtgtg taatagaaaa aacctaataa agcaagacaa aaaatatgaa
    44941 tataaactcc atacatcatg tggggaaaga cagactctat aaaatagcct ggtagataga
    45001 tgattggaaa ttgatagaaa tcataggtcg ttagagatag taaacaaaga acatacataa
    45061 atactgctac aattagaacc catccttgct ttcgccatag aaaaatcatc aataagaaca
    45121 gacaaagtag atagattgac caatttcgca acataaattg aaaaccataa atcatacttt
    45181 cagtcgtttc tccatcaatc acatcaacta ctttatagaa aggtaaacaa tagctcacta
    45241 aaaaaagaag gggactaatt atttttttca tattctctac catggattat tttgcaagta
    45301 agctgaccat ctatctccat atcgaatcaa attaacagta taattagtta ctacccgatt
    45361 ttcacctggt ccaaccttag aaaaacctgt gctatactcc caactacttc ttgcacctgg
    45421 atgaaaccat ttttgctggg atgaatacca accaccttgt ctaacttcca cacttgaaga
    45481 ttctatttcg atatcccttt catggaagtc aactcctcca cttattttag taatattttt
    45541 ttgtacacct ccatcataac tcacccagtg aacaacagta tacaattcca cactataagt
    45601 actatcaaac tgactttgat gagtagtaat gttttgagta taagcgtgca ctttctgtat
    45661 accaaaaata tggtctaata tattcgttgg tttagcaagc ttattgtaac cctcttcaac
    45721 aaaagtagcc ttctctaaat cagcggtata aacagttttt ccttgtacta cttgattgtt
    45781 tatagaagta atacggctat ccacaacaat gggggccacc atatctccat cgctagtttc
    45841 tattttagct gcctctttca cctcatatac aactcgattt tcttcagact gaatcacttc
    45901 taccccattt ttttcaggta tagataatct agtttgccca ttagaaaccg ttatatttgg
    45961 accaatattc ttctcatttg cataagtcgc aataggaaat cctgatagaa ccactgcaca
    46021 cataaggcta ttgactaatt tttttctcat gaatatatcc ttcccatttt caagaggagt
    46081 aaaaatagct aatataagat gaacgtttcc ttatatttca gaataggata tctatctgaa
    46141 agcaattcac actcctatag ataactgttc caaatcttgg ctacatatat ttcaatagtt
    46201 tgctacaaaa aactcctttc taagtctatt gaacatgccc ttattcacct actaatatat
    46261 cacaaaaata aataaataca agactgtttt ttctactagt tttatgatag aatagtattg
    46321 ttagggtaaa tttcatatat aaatgtcgag tgaaaaatcg atggagcaga aatcggtaga
    46381 caaagactcg atttcgttgg tccagcccca caaggctaat caggtatttc acgagtttaa
    46441 aaatctaatg aagtgcccaa tcaactactg attcattgat ttaatacgaa ctatagtgga
    46501 ttgaacctag aaatagtaca ctaaggattc taaaacgttt ctagaaatta atttgacttt
    46561 cctaatctct ttgttcatat cttatttcaa tctactatat ataccaaaat gctaggacaa
    46621 aagtctttaa aatcagagaa acgcatatta tcaggtgttg gaaaaccttg ataatatgcg
    46681 ttttatcata gaaagatcta cttcattttt tactaaaatt aagtttttgt ccctgcatct
    46741 tagagtcctt gccctttaag tgcatctgct aggctggcaa actcttccaa gctgagagct
    46801 tccccacgca cacttggtga caagcctgct tggtccaaag ccttggtcag cttgtccttg
    46861 acctcttcag tcttgccaaa gtaacctgtc aagttattcc acaaggtctt gcggcgatgg
    46921 gtaaaactag ccttggaaac cttaaagaaa aagttctcgt cttccactgc tacagctggc
    46981 actggacggc gcaccatttt caagatggct gagtctacat ttggtgctgg cacaaagact
    47041 gtacgcggca cgataaaggc aaccttggct gtcatgtaat actgcacagc gatagacaag
    47101 ctaccgtaag ccttggtatt tggttgagcc gagatacggt ccgccacttc tttttgcatc
    47161 atgaccacaa actcactaaa aggaatgcca ctctcgatta agtgcatgag aataggcgtt
    47221 gtgatgtagt aaggcaaatt agccactacc ttgattggca ggtcaggatt tttgaaattc
    47281 tggatatgtt gcgccaaatc gaccttgaga atgtcctcgt tgactacagt cacattgtca
    47341 aaatcacgca aggtatctgc caaaatcggt accaaacgat ggtcgatctc aaaggccata
    47401 acttctgctg cacgctcagc caaaaactcg gtcaaggcac caatacctgg cccgatttca
    47461 atgacattga cctggtcatc aatctcagcc gtatccacga ttttttgtag gatattggta
    47521 tccgtcaaga aattttgccc aaaggacttt ttaaaggtaa aaccgtgacg ctccagcact
    47581 gccttggtca cgctataatc tgcaattctc atctattctc ttttctatta tctgttacta
    47641 ctattgtaac acattcccct tgtattttgg gggtgaaaat cataccaaaa aactatcttt
    47701 cctcatgaag aaagatagtc ttggaaatct ttaaaagtgg ccttagcgct tctctcgaaa
    47761 catacgtt
//
LOCUS       DAVBCW010000006        41388 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVBCW010000006 DAVBCW010000000
VERSION     DAVBCW010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 41388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41388
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.006"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            <1..122
                     /locus_tag="VQ514_000277"
     CDS             <1..122
                     /locus_tag="VQ514_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET2499097.1"
                     /translation="SLNQLEQAIVDYIDYYNNKRIKVKLKGLSPVQYRTKSFG"
     gene            complement(251..454)
                     /locus_tag="VQ514_000278"
     CDS             complement(251..454)
                     /locus_tag="VQ514_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2499098.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(447..1529)
                     /locus_tag="VQ514_000279"
     CDS             complement(447..1529)
                     /locus_tag="VQ514_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HET2499099.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(1655..2437)
                     /locus_tag="VQ514_000280"
     CDS             complement(1655..2437)
                     /locus_tag="VQ514_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HET2499100.1"
                     /translation="MVYTSLSSKDSNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(2437..3780)
                     /locus_tag="VQ514_000281"
     CDS             complement(2437..3780)
                     /locus_tag="VQ514_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HET2499101.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQSL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(3879..5195)
                     /locus_tag="VQ514_000282"
     CDS             complement(3879..5195)
                     /locus_tag="VQ514_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HET2499102.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYATVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKKELTAETIVINTGAVSNVLPIPGLATSKNIFDSTGIQSLDKLPEKL
                     GILGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            5388..6609
                     /locus_tag="VQ514_000283"
                     /pseudo
     CDS             5388..6609
                     /locus_tag="VQ514_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
     gene            6808..8382
                     /locus_tag="VQ514_000284"
     CDS             6808..8382
                     /locus_tag="VQ514_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2499103.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
     gene            8646..9434
                     /gene="codY"
                     /locus_tag="VQ514_000285"
     CDS             8646..9434
                     /gene="codY"
                     /locus_tag="VQ514_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HET2499104.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            9434..10009
                     /locus_tag="VQ514_000286"
     CDS             9434..10009
                     /locus_tag="VQ514_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HET2499105.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDFFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            10091..10176
                     /locus_tag="VQ514_000287"
     tRNA            10091..10176
                     /locus_tag="VQ514_000287"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10125..10127,aa:Leu,seq:aag)
     gene            10459..11589
                     /gene="ugpC"
                     /locus_tag="VQ514_000288"
     CDS             10459..11589
                     /gene="ugpC"
                     /locus_tag="VQ514_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HET2499106.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            12090..12848
                     /locus_tag="VQ514_000289"
     CDS             12090..12848
                     /locus_tag="VQ514_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET2499107.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGLSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKVLTGEMTLMKLSGMIYDEGWLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            12935..13447
                     /locus_tag="VQ514_000290"
     CDS             12935..13447
                     /locus_tag="VQ514_000290"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HET2499108.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            13629..14573
                     /locus_tag="VQ514_000291"
     CDS             13629..14573
                     /locus_tag="VQ514_000291"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HET2499109.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYGYL"
     gene            14582..15259
                     /locus_tag="VQ514_000292"
     CDS             14582..15259
                     /locus_tag="VQ514_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HET2499110.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVRSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            15357..16115
                     /locus_tag="VQ514_000293"
     CDS             15357..16115
                     /locus_tag="VQ514_000293"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HET2499111.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            16153..17658
                     /gene="lytC"
                     /locus_tag="VQ514_000294"
     CDS             16153..17658
                     /gene="lytC"
                     /locus_tag="VQ514_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HET2499112.1"
                     /translation="MSVTFFIGEERLKTKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSTEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAESDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTGTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPHYSGKKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            18154..18672
                     /locus_tag="VQ514_000295"
     CDS             18154..18672
                     /locus_tag="VQ514_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HET2499113.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            18802..19308
                     /locus_tag="VQ514_000296"
     CDS             18802..19308
                     /locus_tag="VQ514_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HET2499114.1"
                     /translation="MTKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQAQEKNLYIIGG
                     KQIFQAFEPYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            19510..20742
                     /gene="clpX"
                     /locus_tag="VQ514_000297"
     CDS             19510..20742
                     /gene="clpX"
                     /locus_tag="VQ514_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HET2499115.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            20751..21338
                     /locus_tag="VQ514_000298"
     CDS             20751..21338
                     /locus_tag="VQ514_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HET2499116.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            21349..21729
                     /locus_tag="VQ514_000299"
     CDS             21349..21729
                     /locus_tag="VQ514_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET2499117.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            21781..22671
                     /gene="rapZ"
                     /locus_tag="VQ514_000300"
     CDS             21781..22671
                     /gene="rapZ"
                     /locus_tag="VQ514_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HET2499118.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLAQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            22668..23645
                     /locus_tag="VQ514_000301"
     CDS             22668..23645
                     /locus_tag="VQ514_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HET2499119.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            23642..24553
                     /gene="whiA"
                     /locus_tag="VQ514_000302"
     CDS             23642..24553
                     /gene="whiA"
                     /locus_tag="VQ514_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HET2499120.1"
                     /translation="MSFTVAVKEEILGQHHLSRHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(24587..25555)
                     /locus_tag="VQ514_000303"
     CDS             complement(24587..25555)
                     /locus_tag="VQ514_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET2499121.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWTLAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            25691..26548
                     /locus_tag="VQ514_000304"
     CDS             25691..26548
                     /locus_tag="VQ514_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HET2499122.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            26535..27314
                     /locus_tag="VQ514_000305"
     CDS             26535..27314
                     /locus_tag="VQ514_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HET2499123.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            27338..28690
                     /locus_tag="VQ514_000306"
     CDS             27338..28690
                     /locus_tag="VQ514_000306"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HET2499124.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAIQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            28764..29138
                     /locus_tag="VQ514_000307"
     CDS             28764..29138
                     /locus_tag="VQ514_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HET2499125.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            29140..29988
                     /locus_tag="VQ514_000308"
     CDS             29140..29988
                     /locus_tag="VQ514_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET2499126.1"
                     /translation="MKLAVFTDSSAYLSEETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            30265..31032
                     /locus_tag="VQ514_000309"
     CDS             30265..31032
                     /locus_tag="VQ514_000309"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HET2499127.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDDMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            31044..32228
                     /locus_tag="VQ514_000310"
     CDS             31044..32228
                     /locus_tag="VQ514_000310"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HET2499128.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQGKEVNPQAITEKYQ
                     SLTIHDKKEIQINGDILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            32225..34096
                     /locus_tag="VQ514_000311"
     CDS             32225..34096
                     /locus_tag="VQ514_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET2499129.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELDRAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQAVSFAYENKPILQD
                     FNLLVQAKDRIGIVGDNGVGKSTLLNLIAGSLEPTKGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDSKIRPFFGHYTDYLDEKAFETDMANQVQKVEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(34239..35423)
                     /gene="mntE"
                     /locus_tag="VQ514_000312"
     CDS             complement(34239..35423)
                     /gene="mntE"
                     /locus_tag="VQ514_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HET2499130.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPISEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            35727..38423
                     /locus_tag="VQ514_000313"
     CDS             35727..38423
                     /locus_tag="VQ514_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HET2499131.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            38436..39227
                     /gene="yghU"
                     /locus_tag="VQ514_000314"
     CDS             38436..39227
                     /gene="yghU"
                     /locus_tag="VQ514_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HET2499132.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTENVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            39343..39753
                     /locus_tag="VQ514_000315"
     CDS             39343..39753
                     /locus_tag="VQ514_000315"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET2499133.1"
                     /translation="MEKKIVKDILFLSQVSHPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGMQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            39765..41360
                     /locus_tag="VQ514_000316"
     CDS             39765..41360
                     /locus_tag="VQ514_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499134.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPKSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTN
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESIKGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKVQ"
BASE COUNT        12131 a         7754 c         9468 g        12035 t
ORIGIN      
        1 agtcgcttaa tcaattggaa caagctattg tagactatat tgattactac aacaataaac
       61 gaattaaggt aaaactaaaa ggacttagtc ctgtgcaata cagaactaaa tccttcggat
      121 aaattaattg tctaactgtt tgggggcagt acatattcaa cacctgatac tatgcgtttt
      181 attgtgggaa gattttcctc attttctagt gaaattgaac tttttctaac atcttttgat
      241 agatgacttc ttaatcaatt atcatttcca ttgttgtttc ttggggtttc atcccttgtc
      301 tttgataaaa gcggagagca ccctcgttat cattccagac acttagggtt agattattgc
      361 agccaatttc tttagcataa tagcagaaaa atgacaataa aatagcccct agatattttg
      421 aaaggaaagc ataggggcta tatagtttaa tcaaaataaa gcaaatcttt gctggtgctt
      481 gtaaagaggt cgaagccatc cttggtaaca acaccgcagt cttcaatacg aacaccgact
      541 ttaccaggga tatagatacc tggttcaaca gagaagcaca tgccttcttc gatgaccatg
      601 tcgtttcctt ccatgataga tgggaattca tggacatcca taccgatacc atgcccgaga
      661 cggtggttga agtactcacc ataaccagct ttttcgatga cctcacgggc agcgcggtcc
      721 acttcatgag cagtcacacc tggcttgata aagtcaagag cagcttgttg ggcttcaaga
      781 gtcaagttgt aaatatcttt cttgaattgg tctggtttgc cgacagcgac tgtacgagtc
      841 atatctgacg catagccatt gaccagaaca cccaggtcaa agaggagaag agcatcattt
      901 tcaaccttat tagctgctgg aatgccgtgt ggattcgcag cattatcacc agtcaagacc
      961 atggtatcaa agctcatttc ataaccttca cgtttcatgg caaagtcgat ttgtgcgatg
     1021 atatctgtct cagtcttatc aagagaaata ttgtcaaaac caacatgaac agccttgtca
     1081 gcataaagac ctgcaaccat cattttttgc acttcatcag ctgatttgat gaggcgcatg
     1141 cgttggatac gaggagtgag gttgtcaaac tcagcagtct caaaaactgt tttcaaacca
     1201 tggtatttgg tcaagatgag attgtcaaac tcaacagcga cacgtttgaa gtcaagttgt
     1261 ggaagagcat gtttgatttt ttgccatgga ttttcagaat cgacatagcc cactactggg
     1321 aaggaaacgg tgctacttgc acgttctact tcaagagctg ggacaaagag gagaggttcc
     1381 tgatccgcta ggacaaagag gaacatttgg cgttcatggg gatcactgta aaaaccagtg
     1441 aggtaattga ttgtgacggg gtcagatacg acagcgacgt ctagtttttc tgattcaaga
     1501 tatgttagga tttgttgtaa tttagacata tgctaccttc tttctacccc tctattttgg
     1561 caaaaaatga gagaaaatgc aagggagaag ggtaaaagaa aagacaaaaa actccgacaa
     1621 gataacggag ttctttgata tcattttcct ttgtttaaga aaagtcagcc ttgcttttga
     1681 cgtcactgta ctcttcagcg atttcttggc tgagaatgtc ctcataggca gggagttgga
     1741 tgtcctgacc atatttcttc ttgagggcag tagtaactag gcgataagcc agattggcat
     1801 tacgagagag gataggcccg tggaagtagg aaccaaagac attcttataa tgaacccctt
     1861 caccgacctt ttcttcgttg tttccatttc catagacaac ctgtcccagc ggtttttggt
     1921 catcagagag gaaggtacga ccttggtgat tttcaaatcc atagtaggtt tcatcgaaat
     1981 cttcattgtg aatcttgatg tcaccgataa aacggttatt ggtctggttg agcgtgtagt
     2041 gtcccatgac ccctagccct tcgatacgtt ttcctgaagc ttcaacataa tattgaccca
     2101 atagttggaa accaccgcag atagccagaa ctacaccgtc gttttggatg tagttgtcaa
     2161 tgctctcttt tttagcaggt aggtcgtctg caatgatact ttgttcaaag tcttgaccac
     2221 caccgaaaaa ggcgatgtcg tagtgatttt catcaaagtc atcatggaga gaaacgatgt
     2281 caacggtcac atgggctccc agtttttcag ccacatactt gagcatgagg atgtttccat
     2341 tgtccccgta ggtattcatg agatttccgt agaggtgggc aatgttgagc tgataggggt
     2401 aattgctatc ttttgaggaa agtgaagtat aaaccattag ttcatctcct ttctaacaat
     2461 ctgacgacta gccagcagtt cacgaaattc cagcatggca gtataagttg ccagaatata
     2521 ggcatgcttg cagtcttgat tctcaatggt cttgagaact tgctccagat tactcgtttc
     2581 agtgattttc tcagctggat agccagtcac tcggaggcga cgagcgattt cagaatgacg
     2641 aacaccgcca gcgttgattt caggaatgtc catgtcagtg atttgctcaa agtctgcatc
     2701 ccagatccag ctagtgtcaa ttccatctgc atagttggca ttaaggagga cagataggct
     2761 aaatggataa ggtgctagtt tgatcatttc gatagcttgg gttgcaccga ctggattttt
     2821 aatcaagaca agggtacatt ccttgtcacc gatatgaaag gtttcttggc gtccaaagac
     2881 agcacggctc ttgtcaaatc cctgtttgat gagttgggaa tctgcgccga ggaaacgggc
     2941 gatggccaca gcagctaggg cgttatagat attatagagc ccgccgattt ggataccgta
     3001 ttcttggccg tctatgacaa agcgagagcg attgttggtc aactcaacca gttttgtcaa
     3061 acgatagtcg agatcaggac gtttacatcc acaaccttca cagatatagg cacccaagtt
     3121 tgcataggta ttatgctcat atttgaggat gccttggcag tcaggacaga gaatcccttc
     3181 ggtattgtag tgagccagtt gggctggtcc tttttccaag tcaaaaccaa aatactctat
     3241 agggtttgga atagttggct tgtagaaaag tggactgtct ccgttaagga gaacagtagc
     3301 agttggaact tttcgaatgg catccaatat catgttatag gtagtataga tttcaccgaa
     3361 acggtccatc tggtcacgga agatattagt aatgacaaaa agactaggct ggatatagtc
     3421 acagatacga gatagactgg cttcgtcaat ttcgaggacg gcaatatttt tcccagtttt
     3481 tgaagatttg gctgttagga aggttgttgc aatccctgta atcatgttgg caccgcttgg
     3541 gttggttaga acttgaccat aaacctcttt taaaatgccg acagtgaggg cagttgtcag
     3601 ggtttttcca tttgttccag tgacaacgac aatctcgtag ttcttagcta ggctttgtaa
     3661 aatatcttta tcaaattgaa gggcgacttt ccctgggagc gtacttccac gtccaagacg
     3721 gcttaaaacg aagtgggaag aacgcccagc aagaaggccc aaagtagttt ttaagttcat
     3781 gtttctatta tatcacaaaa gagggaaaag acagatttgg tgtttcgtag aagtaaaaac
     3841 agcccaaaga gggctgtcag ttaagatgag atttcaactt aaatcgcaaa caagtcattc
     3901 aagttctcag ccaaggttgg gtgagtgaag atttgttttg tgaagtaagt gtaaggaatc
     3961 ttgttgtcca tagcaacagt aatgatgttg atgatttctt gagaaccttc tgagaagatg
     4021 cttgcaccaa gaatttcttt tgtttcagta ttaacaacag ctttgaaagc tccgcgaagg
     4081 tctccattta cgtgaccacg aggcatggct gcaacaggga tttctttcac tgcgtatgga
     4141 agtttcaaat cagctgcttg gctttcagtc aaaccaactt gtgaaagtgc aggtgtgatg
     4201 aacatagtat ttggcacatt gagacggtcc tcaagtgtgt agctgccatc tccagcaagg
     4261 tagctgtaaa caacacggaa gtcatcaagt gaaatgtaag taaattgaag gccaccgttg
     4321 acatctccaa ctgcaaagac accaggaacg tttgtttgac agtgtttgtc tactttaata
     4381 gcaccacgtt cagttagttc aatatctgta ttttcaagtt gaagtggttc tacatttggt
     4441 ttgcgtccag ttgcgtagag aagggcgtcg aaacggtaag tttcgtcttc agttacgaca
     4501 agcacttggt caccatcgtt tttgatttca gtagtatgga tattttgaag caattcaatg
     4561 ccatcttctt ccatgtattg tttagcaaga gctgcgatgg aaggttctgc acgaggtagg
     4621 aatgtatcca aggcatctag gactgtgacc ttgcttccaa gtttgttgta aaggccggca
     4681 aattcaagac cgatatttcc gccaccaagg attccaagtt tttcaggtaa tttgtccaag
     4741 ctttggatac ctgttgagtc aaagatgttt ttgcttgtag caagtccagg aattggcaag
     4801 acgtttgaaa cagcaccagt gttgatgacg attgtttcag cagtcagttc tttcttttca
     4861 tcaccagctt ggatttcgat gactttattt gaaaggaagt gagcttccgc atcaaagata
     4921 tctacgcctg taccagcaac agtcgcatag tttttaccgt tgaggcgacc agtgatcgtg
     4981 tttttagtag caatcacttc ttcaaaagac aagtcctttt cagcagcaac tagcaaggtt
     5041 ttagttggga tacaaccaat gttgatacaa gttccaccgt acatagcctt gctgcgttca
     5101 acgagggcaa cttttttgcc agctgaagcc aatttacctg ctagtgtttt accagcttta
     5161 ccaaatccaa taacaattaa atcgtatgtt aacatttatc ttcctcctat tcgaatttca
     5221 ttctagcaca agaaaaaaac ttttgcacaa ttctgctacg gaaaagacag aaaaagataa
     5281 aaacgctttc cagacaaaaa actagtaatt ttttgacaag cttgataaac taaaatccta
     5341 tctattttta tataagaaag aataaatgtt cgaaaatagg aggttttatg aaatcgaatc
     5401 gttatattat tgcctttgct ggggtcattt tacacttaat gctgggttcg acttatgctt
     5461 ggagtgttta tcgtaaccct attattgaaa aaacgggatg ggatcaggct tctgttgcct
     5521 tcgcctttag tctagcaatc ttttgtttgg gcttatcggc tgcatttatg gggcgtttgg
     5581 tagaaaaatt tggtccgaaa gtcatgggga gtctatctgc ttttctatac gcaggtggaa
     5641 atatcttaac aggatttgca atagaccgtc aggaactgtg gttgttgtat ctagcttatg
     5701 gcattttagg tgggcttggt ttgggagcag gctatattac ccctgtgtca acgattataa
     5761 aatggtttcc tgataaacgt ggtctcgcaa caggtttagc gattatgggg tttggttttg
     5821 cttctttatt gactagtccc atagcgcaac acctcatcgc aggggtaggg attgtagaaa
     5881 ctttttatat tttaggagca agttacttta ttatcatgct cctagcttca caattcatta
     5941 agcgtccaaa tgagcaagag cttgcaattt tatcttcttc agggaaagaa aaaacagcct
     6001 ctttgacgca aggaatggct gcaaatcagg ctctaaaaag caatcggttt tatatgcttt
     6061 ggattatttt ctttatcaac atagcttgtg gtttaggctt aatttcagcg gcatcgccaa
     6121 tggcacagga gatggctggc ttgtctacaa gtcatgcagc agtaatggtg ggtgttttgg
     6181 ggattttcaa tggatttggt cgcttgctct gggcgagttt gtctgactat atcggtcgcc
     6241 ctctaacctt tagtatatta ctgcttgtca atcttttctc tctcactttg gctctttaca
     6301 gattccgttt tatttgtagt tgctatgtct attttgatga cttgctatgg agctggtttt
     6361 tctttgattc cagcttatct cagtgatatt tttggaacca aggaattggc cgctctgcat
     6421 ggttatattt taacagcttg ggcaatggct ggtttagcgg gacctatttt attagcagag
     6481 acttataaaa tggctcattc gtacacacaa accttgttcg tttttctcat tttatacagt
     6541 atcgccttgg ctttgtctta ttatctaggt cgttcaatca aaaaagaaag tcaaaaaccg
     6601 cttacatgat tttctgtaga aatggggcta tcttttgctc tggtcttgtg ttatactaga
     6661 taggttgcaa agaaaacagt acttttcttt tgtggaaaag aagcaacacg attttatatc
     6721 tagttatatg aaaatacgtc ataaaaagaa aagtataaac taagccctat agggtggctt
     6781 cgcaccacct ttagaaagaa gaataacgtg aaatttaatg aattaaactt gtctgctgat
     6841 ttgctagcag aaattgaaaa agctggtttt gtagaagcta gtcctatcca agaacaaact
     6901 attcctttgg cccttgaagg caaggatgtt atcggtcaag ctcagactgg tacaggaaaa
     6961 actgcagcct ttggcttgcc tacccttgaa aaaatccgta cagaagaagc gactatccaa
     7021 gctttggtca tcgctccaac tcgtgaacta gctgtccaaa gtcaagaaga actcttccgc
     7081 tttggtcgta gtaagggagt caaagtccgt tcagtatatg gcggatcaag cattgaaaaa
     7141 caaattaagg ctcttaaatc tggtgcccat atcgtggtgg gaactccagg tcgcctcttg
     7201 gacttgatta aacgcaaggc cttgaaatta caagacattg aaacccttat ccttgacgaa
     7261 gcggatgaaa tgcttaacat gggcttcctt gaagacatcg aagccattat ttcccgtgta
     7321 cctgagaacc gtcaaacttt gcttttctca gcaactatgc cagatgccat caaacgtatc
     7381 ggtgttcagt ttatgaaagc ccctgaacat gtcaagattg cggctaagga attgacaaca
     7441 gaattggttg accagtacta tatccgtgtt aaggaacaag aaaaatttga caccatgact
     7501 cgtctcatgg atgtggcaca accagaactc gctattgtat ttggtcgtac caaacgccgt
     7561 gtggatgaat tgactcgtgg tttgaaaatt cgtggcttcc gtgcagaagg aattcatggc
     7621 gacctagacc aaaacaaacg tcttcgtgtc cttcgtgact ttaaaaatgg caatcttgat
     7681 gttttggttg cgacagacgt tgcagcgcgt ggtttggata tttcaggtgt gacccatgtc
     7741 tacaactacg atattccaca agatcctgag agttatgttc accgtatcgg tcgtacaggt
     7801 cgtgctggta agtcaggtca atctattact tttgttgctc caaacgaaat gggttacctt
     7861 caaatcattg aaaacttgac taagaaacgc atgaaaggtc tcaaacctgc aagtgtagaa
     7921 gaatccttcc aatcaaaaaa acaggtagct ctcaagaaaa tcgaacgtga ttttgcagat
     7981 gaaaccattc gtgccaactt tgagaaattt ggtaaggatg ctcgcaaatt ggctgctgag
     8041 tttactccag aagaattggc aatgtatatc ttgagtctga cagtccaaga cccagatagc
     8101 cttccagaag tggagattgc acgtgaaaaa ccactaccgt ttaaaccatc aggtaatggt
     8161 ttcggtggta aagctaaggg aggtcgtgga ggccgtcgtg gggacgaccg tcgagagcgt
     8221 gatcgccgtg gcaatggtcg ccgtgatgag ttcaaaaaag gaagtcgtgg caacgatcgt
     8281 tttgataagg aaaaacgtta ccgtaaggat aataaaaaac cacgcaatac tttaagcgaa
     8341 aagcaaacag gctttgttat tcgtaacaaa ggcgataaat aagaaaaagc ggttcaaaat
     8401 gaatcgcttt tttatataag gagaccgaat gtgaaaagac aaataaaatt actatattat
     8461 cttttataat gttatgatac acaaaaatag gcaacttgtc aatagaaaag gaacaattta
     8521 attaaaaata ttctggtttt taattaaatt gtaagtcaat gcaagtcaat aaggaatttt
     8581 cagataatta ttgaaaaaga gacgttttta tgttataatg atagcgatta aattcgaggt
     8641 gaaaaatggc acatttatta gaaaaaacta gaaaaattac atcaattttg aaacgctcag
     8701 aggagcagtt gcaggatgag ctcccttaca acgctattac gcgtcagtta gcggatatta
     8761 ttcattgcaa tgcctgcatt atcaatagta agggacgtct gcttggctat tttatgcgtt
     8821 ataaaacaaa tacagatcgc gtagagcaat tcttccaaac taagattttc ccagatgact
     8881 acgttcaagg ggctaatatg atttacgaaa cagaagcaaa cttgcctgtt gagcatgata
     8941 tgagtatttt ccctgttgag agtagagatg attttccaga tggcttgacg actattgcac
     9001 cgattcatgt atcggggatt cgccttggtt ctttgattat ttggcgtaat gataaaaaat
     9061 tcgaagatga ggacttggtt cttgttgaga ttgccagtac cgttgttggg attcagcttc
     9121 ttaacttcca acgtgaagaa gatgagaaaa atattcgtcg tcgtactgct gtcaccatgg
     9181 cggtcaatac cctttcttac tccgaactcc gtgctgtttc agcaatttta ggggaattaa
     9241 atggaaatga agggaagttg actgcgtcag tgattgcaga tcgtatcgga atcactcgct
     9301 ctgtgattgt caatgctctt cgtaaacttg agtctgcggg gattattgaa agtcgctcac
     9361 ttggaatgaa gggaacctat cttaaggtct tgatttcaga tatttttgaa gaagtgaaga
     9421 aaagagatta ctaatgacaa aggctttaat ttcgattgat tatacagaag attttgttgc
     9481 tgatagtggg aagctgacag caggtgctcc agctcaggcg atttcggatg ccatcagcaa
     9541 ggtaactcga ttagcttttg aacgaggaga ttatatcttc tttactattg atgctcatga
     9601 agaaaacgat tttttccatc cggaaagtaa gttatttcct cctcataatc tgattgggac
     9661 gagtggacgg aatttatatg gagatttggg gatcttttat caagagcatg gttcagacag
     9721 tcgtgtcttt tggatggata aacgccatta ctcagctttt tcagggactg acctagatat
     9781 tcgtttgaga gagcgtagag tgtctaccgt tatcttaaca ggtgtcttga cggatatctg
     9841 tgtcctacat acagctatag atgcctataa tctaggatat gacatcgaga ttgttaaacc
     9901 agctgttgct tccatctggc ctgaaaatca tcaatttgcc ctaggtcatt tcaaaaatac
     9961 actcggagct aagttagtag atgaaaatct aaatgaactt tctgagtaac ctggttggtg
    10021 aaaatgaaaa aatttgaaaa aaagtgttga caagcatccc aaaagttgat atactagtaa
    10081 agtaatcgat gcggggatgg cggaattggc agacgcgcag gactaaggat cctgtgaccg
    10141 ctttaggtcg tgagggttca agtccctctc tccgcatagg aaaagagtta gctaaagcta
    10201 gctcttttgt atttctctaa aaaattagaa tccagtcaaa tgtagtggag aatcagcgaa
    10261 aatatgggaa aactgttcct tgtttctgct tttgtcatag aagaataata aagattatct
    10321 tcttcaaatt ctccgatatt ctctaaagtt ttgtgcaagt tgcacagaac ttttttattt
    10381 ttttggtcat cttgccatag aaatataaag cgttttcatc tataatataa ttatcaaaag
    10441 acaaaaggag ttcacctcat ggtagaattg aatcttaaaa atatttacaa aaaatatcca
    10501 aacagcgaac actattcagt tgaagatttc aacttgaaca tcaaagataa agaatttatc
    10561 gttttcgtag gaccttcagg atgtggtaaa tcaactacac tccgtatgat tgctggtctt
    10621 gaagacatta cagaaggtac tgcatctatc gatggcgtgg ttgtcaacga cgtagctcca
    10681 aaagaccgtg atatcgccat ggtattccaa aactacgctc tttacccaca catgactgtt
    10741 tatgacaaca tggctttcgg tttgaaattg cgtaaataca gcaaagaaga cattaacaaa
    10801 cgtgttcaag aagcagctga aatacttgga ttgaaagaat tcttggaacg taaaccagct
    10861 gacctttcag gtggtcaacg tcaacgtgtt gccatggggc gtgcgattgt ccgtgatgcg
    10921 aaagtattct tgatggacga acctttgtca aacttggatg ccaaacttcg tgtatcaatg
    10981 cgtgctgaaa tcgctaaaat tcaccgtcgt atcggagcta caactatcta tgtaactcac
    11041 gaccaaacag aagcgatgac acttgcagac cgtatcgtta ttatgtcagc tactaagaac
    11101 cctgctggta caggtactat cggacgtgta gaacaaatcg gtactcctca agaagtttac
    11161 aaaaatccag ttaacaaatt cgttgcagga ttcatcggaa gcccagctat gaacttcatc
    11221 aacgtgaaat tggttggtag cgaaattgtt tctgacggtt tccgtttgaa agtgccagaa
    11281 ggagcattga aagttcttcg tgaaaaaggc tacgaaggaa aagaattgat ctttggtatc
    11341 cgtccagaag acgtgaatgc agaacctgct ttccttgaaa cattcccaga ctgtgttgta
    11401 aaagcgacta tctctgtatc agaactgctt ggttcagaat ctcaccttta ctgccaagtt
    11461 ggtaaagacg agtttgttgc aaaagttgat gctcgtgact acttgcaaac aggtgcaaca
    11521 gttgagcttg gatttgactt gaacaaagca cacttcttcg atgtagaaac tgaaaaaaca
    11581 atctactaaa ataaataaaa ttcaaagcac tacaagaaaa gatatctctt tatcaattgt
    11641 agtggagaga tatcagttaa tctagggaga gaaacaaaat gcttctctcc tttttgctag
    11701 ggaagtcata ttatgcatct atattgtgat gctctttaat actcttcgaa aatctcttca
    11761 aaccacgtca acgtcgcctt gccgtacgta tgattactga tttcgtcagt tttatctgca
    11821 acctcaaaga tgtactttga gcagcttacg gctagtttcc tagtttgctc tttgatttcc
    11881 attgagtatt atttgtgggt accatctaca agtgaagcta tatgcgtaaa ctacgtgagc
    11941 aattgaattc gaactagaga ggtaataata aatttatgct atagttatgg tgacttgtat
    12001 gcttttgatt ctagtttatc aaataataga ttagaattgt cagataatat cattttgtgt
    12061 tataatgaag aaaaaacaga ggtgttcaaa tgtcagaagc aggtcataag tttttagcaa
    12121 aattggggaa aaaacgctta cgtccaggtg gaaagcgtgc tacagattgg ttaattgcag
    12181 aaggaggatt gtcaaaagaa aagagaatac tagaggttgc gtgtaatagg ggaactacag
    12241 caattgagtt ggcacagcgt tttggttgca agataactgc tgttgatatg gatgctcaag
    12301 ctttagaagt ggctaaaaaa tctgctggaa cggcaggtgt tgctcattta atcagttttg
    12361 aaagagcaaa tgcaatgaaa cttccttatc aagatgctag ttttgatatt gttataaatg
    12421 aagctatgct gactatgcaa gccgatcaag ctaagaaaaa atgtgtaatg gaatatctaa
    12481 gggtattaaa acctggaggt cttcttttga cacatgatgt gcttcttaag gaagctaaag
    12541 agtctatcag acaggaatta tcacaagcaa ttcatgtaaa tgtaggtcct ttaactcaag
    12601 atggttggga acaggtgatg atagaatcag gttattgtga tgtgaaagta ttgactggtg
    12661 aaatgacatt aatgaaatta tcgggtatga tttatgacga aggttggcta ggaactttga
    12721 aaatttgtgt aaatgcttgt aaaaaggaga atagaaagca gtttttaact atgtataaaa
    12781 tgtttgctaa gaataaacag aaattgggct ttattgcgat ggctagttat aaatcgtcaa
    12841 aacgttagat aattattgaa gttaactttt ccttttttct ttcttaaaaa atatgctata
    12901 atagagagtg aaaaactttg aaagaaagaa aaagatgaat ttaaaagatt acattgcaac
    12961 aattgaaaat tatccaaagg aaggcattac cttccgtgat attagtcctt tgatggctga
    13021 tggaaatgct tatagctacg ctgttcgtga aatcgttcag tatgctactg acaagaaagt
    13081 cgacatgatc gtgggacctg aagctcgtgg atttatcgtg ggttgtccag ttgcctttga
    13141 gttgggaatt ggttttgcgc ctgttcgtaa gccaggtaaa ttgccacgcg aagttatttc
    13201 tgctgactat gaaaaagagt acggtgtcga taccttgact atgcacgcgg atgccattaa
    13261 gccaggtcaa cgtgttctta ttgtagatga ccttttggcg acaggtggaa ctgttaaggc
    13321 aactatcgag atgattgaaa aacttggtgg tgttatggca ggttgtgcct tccttgttga
    13381 attggatgaa ttgaacggcc gtgaaaaaat cggtgactac gactacaaag ttcttatgca
    13441 ttattaatga aaacagtccc tagggctgtt ttctctacac taggatataa aaatagacta
    13501 taactagtta gagaaaaact ataattgaaa actatatctt cttgcagtat aataaaagga
    13561 ctaagtgttt gagatttgtc ttcaaacaca tgcaattatt cctgagagag tacagttagg
    13621 agagggttat gccgattcga attgataaaa aattgccagc tgttgagatt ttacggacag
    13681 agaatatctt tgtcatggat gatcaacgtg ctgcccacca agatatccgt cctttgaaga
    13741 ttttaatttt aaatctcatg ccacagaaaa tggtcacaga gacccagttg ttgcgtcact
    13801 tggctaatac acccctacaa ctggatattg attttctcta tatggagagc caccgttcta
    13861 aaacaactcg ttcagagcac atggagacct tctataaaac ttttcctgaa gtcaaggatg
    13921 agtattttga tgggatgatc atcacgggtg ctccagttga gcatttacca tttgaggaag
    13981 tggactattg ggaggaattt aggcagatgc ttgagtggtc taagacacat gtctattcga
    14041 cccttcatat ctgttggggg gctcaggctg ggctttatct gcgctatggt gtagaaaaat
    14101 accagatgga cagtaagcta tcaggtattt atcctcagga caccctaaaa gagggtcacc
    14161 ttctatttag aggctttgat gatagctatg tatcccctca ttcacggcac acggagattt
    14221 ctaaggaaga ggtcttaaac aagaccaatc tcgagatttt atcagaagga cctcaggttg
    14281 gggtttctat tttggccagt cgtgatttac gagaaattta tagttttggt catttggagt
    14341 atgaccgtga taccttggca aaagagtatt ttcgagatcg tgatgcaggt ttcgacccac
    14401 atattccaga aaattacttt aaagatgatg atgttaatca ggtaccttgt ctttgttggt
    14461 cttcatctgc agccctcttt ttcagtaatt gggtaaacca tgcggtctat caggagacgc
    14521 cttttgattg gagaaagata gaagatgatg catctgcata tgggtattta taagaggaat
    14581 tatgacatat ttagacgctt ttaaatcagg taccttggtt ttaccgagtg ccctgctctt
    14641 gcattttaag gaactctttc cttctagcga cgattttctg gtttggcaat ttttctattt
    14701 gcaaaatacg acaggcttag aagaaatgtc gccaagccag attgctgaaa ggattggcaa
    14761 ggaaatttcg gatgtcaacc agtccatttc taatctgacg gaaaggggac tgctccagta
    14821 tcgtactatc gaattaaatg gcgaaattga attgctcttt gatgctagtt tggccttgga
    14881 acgtttggat gacctgtttg gagcagttcg ttcaagttca gaccagctaa cacctcaaaa
    14941 ccagctcaag gatttggtgg aaaccttcca gcaggagttg ggacgattgt tgacgccttt
    15001 tgagattgag gatttgacca agacactaaa ggaagatgga accagtgctg acttgattaa
    15061 ggaggctctt cgtgaagctg ttttgaatgg aaaaccaaac tggaagtaca ttcaggcgat
    15121 tttgagaaac tggcgccatg aaggaatcaa gagtgtggct caaattgagg ccaagagagc
    15181 agaaagagaa gcaagcaatc ctcagttgac acaggtatct gcagatttca gaaatgccat
    15241 ggatctctgg aaggattaat ccatgcaagt aggcttgaaa tccgagtaag atttgcaagc
    15301 tgtgtataat tgtgatagaa taaatagaaa ataaattgaa aaaagaggta tgtgaaatgt
    15361 cacgtaaacc atttatcgct ggtaactgga aaatgaacaa aaatccagaa gaagctaaag
    15421 cattcgttga agcagttgca tcaaaacttc cttcatcaga tcttgttgaa gcaggtatcg
    15481 ctgctccagc tcttgatttg acaactgttc ttgctgttgc aaaaggctca aaccttaaag
    15541 ttgctgctca aaactgctac tttgaaaatg caggtgcttt cactggtgaa actagcccac
    15601 aagttttgaa agaaatcggt actgattacg ttgttatcgg tcactcagaa cgccgtgact
    15661 acttccatga aactgacgaa gatatcaaca aaaaagcaaa agcaatcttt gcgaacggta
    15721 tgcttccaat catctgttgt ggtgaatcac ttgaaactta cgaagctggt aaagctgctg
    15781 aattcgtagg tgctcaagta tctgctgcat tggctggatt gactgctgaa caagttgctg
    15841 cctcagttat cgcttatgag ccaatctggg ctatcggtac tggtaaatca gcttcacaag
    15901 acgatgcaca aaaaatgtgt aaagttgttc gtgacgttgt agctgctgac tttggtcaag
    15961 aagtcgcaga caaagttcgt gttcaatacg gtggttctgt taaacctgaa aatgttgctt
    16021 catacatggc ttgcccagac gttgacggtg cccttgtagg tggtgcgtca cttgaagctg
    16081 aaagcttctt ggctttgctt gactttgtaa aataatcagt aagtagcaaa agctaggtgg
    16141 aacagcattc agatgtctgt tacatttttt ataggagagg aaagattgaa aacaaaaatt
    16201 ggattagcaa gtatctgttt actaggcttg gcaactagtc atgtcgctgc aaatgaaact
    16261 gaagtagcaa aaacttcgca ggatacaacg acagcttcaa gtagttcaga gcaaaatcag
    16321 tcttctaata aaacgcaaac gagcacagaa gtacagacta atgctgctgc ccactgggat
    16381 ggggattatt atgtaaagga tgatggctct aaagctcaaa gtgaatggat ttttgacaac
    16441 tactataagg cctggtttta tattaattca gatggtcgtt actcgcagaa tgaatggcat
    16501 ggaaattact acctgaaatc aggtggatat atggcccaaa acgagtggat ctatgacagt
    16561 aattacaaga gttggtttta tctcaagtca gatggggctt atgctcatca agaatggcaa
    16621 ttgattggaa ataagtggta ctacttcaag aagtggggtt acatggctaa aagccaatgg
    16681 caaggaagtt atttcttgaa tggtcaagga gctatgatgc aaaatgaatg gctctatgat
    16741 ccagcctatt ctgcttattt ttatctaaaa tccgatggaa cttatgctaa ccaagagtgg
    16801 caaaaagtgg gcggaaaatg gtactatttc aagaagtggg gctatatggc tcggaatgag
    16861 tggcaaggca actactattt gactggaagt ggtgctatgg cgactgacga agtgattatg
    16921 gatggtgctc gctatatctt tgcggcctct ggtgagctca aagaaaagaa agatttgaat
    16981 gtcggctggg ttcacagaga tggtaaacgc tatttcttta ataatagaga agaacaagtg
    17041 ggaaccgaac atgctaagaa aatcattgat attagtgagc acaatggtcg tatcaatgat
    17101 tggaaaaagg ttattgatga gaacgaggtg gatggtgtca ttgttcgtct aggttatagc
    17161 ggtaaagaag acaaggaatt ggcgcataac attaaggagt taaaccgtct gggaattcct
    17221 tatggtgtct atctctatac ctatgctgaa aatgagaccg atgctgagag tgacgctaaa
    17281 cagaccattg aacttataaa gaaatacaat atgaacctgt cttaccctat ctattatgat
    17341 gttgagaatt gggaatatgt aaataagagc aagagagctc caagtgatac aggcacttgg
    17401 gttaaaatca tcaacaagta catggacacg atgaagcagg cgggttatca aaatgtgtat
    17461 gtctatagct atcgtagttt attacagacg cgtttaaaac acccagatat tttaaaacat
    17521 gtaaactggg tagcggccta tacgaatgct ttagaatggg aaaaccctca ttattcagga
    17581 aaaaaaggtt ggcaatatac ctcttctgaa tacatgaaag gaatccaagg gcgcgtagat
    17641 gtcagcgttt ggtattaagc gatgatttga aagagggatg tgatagtagc accctctttt
    17701 tctttgtttt atgatagttc atcctcgagt aaattcaagt tcttgctcgg aaatgaagct
    17761 tatatagtag attgaatata gacaaatacc ttgtgattgg taaaacattt tagaaattca
    17821 tttacctttc ctaatcgact tggtttcatc ttatttcaat ctattatagt attggggaat
    17881 ttcttcaaac cacatcagct tggtcagttc tacctgcgac ctcaaaactt gtgctttggt
    17941 caagctgggt ttagtttcct agtttgctga tggatttcca ttgactataa gcatccaacc
    18001 ctctttttgt cttctaaaga attcttaaat tatcagtcta ttgcaacttt tctcatataa
    18061 gttctttgtc ttgctattgg ttttccttag tagtatacta aggtagtaat cattaagaag
    18121 tggttacaaa aaataatgaa tgaggtaaag aaaatggtag aattgaaaaa agaagcagta
    18181 aaagacgtaa catcattgac aaaagcagcg ccagtagcat tggcaaaaac aaaggaagtc
    18241 ttgaaccaag ctgttgctga tttgtatgta gctcacgttg ctttgcacca agtgcactgg
    18301 tatatgcatg gtcgtggttt ccttgtatgg catccaaaaa tggatgagta catggaagct
    18361 cttgacggtc aattggatga aatcagtgaa cgcttgatta cactcggtgg aagcccattc
    18421 tctacattga cagagttcct tcaaaatagt gaaatcgaag aagaagctgg tgaataccgt
    18481 aatgttgaag aaagcttgga acgtgttctt gttatctacc gttacttgtc agaacttttc
    18541 caaaaaggtt tggatgtcac tgatgaagaa ggtgacgatg tgacaaacgg tatctttgca
    18601 ggcgctaaaa ctgaaacaga taaaacaatt tggatgcttg cagccgaact tggacaagca
    18661 cctggtttgt aagaaataag gaaacataga taaatctgta ggaaatttct tacagatttt
    18721 ttctattttg taagctattc caaaccagtc tttttttgag ttttttgata aaatagtact
    18781 atcagtgaaa aggatggaag catgactaag aaaatcgtag ctatttgggc ccaggatgaa
    18841 gagggtgtga ttggtaagga aaatcgtctg ccttggcatt tgccagcaga attgcagcac
    18901 tttaaagaaa caactctgaa tcatgctatc ttgatggggc gtgtgacctt tgatgggatg
    18961 gggcgtcgct tgcttccaaa acgggaaacc ttgattttga cgcgtaatcc ggaagaaaag
    19021 atagatgggg ttgctacttt tcaggacgtc cagtctgttc ttgactggta tcaggctcaa
    19081 gaaaagaatc tctacattat cggtgggaag caaatttttc aggcttttga accttacctt
    19141 gatgaagtga ttgtcactca cattcatgct cgggtggaag gagataccta tttccctgaa
    19201 gaacttgact tgtctctttt tgagacagtt tcaagcaaat tttacgccaa agatgagaag
    19261 aatccttatg attttaccat ccaataccgc aagagaaagg aagtctaatg gaacggagtt
    19321 tatttggtct attcacggct tttctgtgtt tcatctgctt tctggcagga gcacaagctt
    19381 ttcgtaaaaa gcgttacggg ctttctatcc tcctttggtt aaatgccttt accaatctgg
    19441 tcaatagtat tcatgctttt tacatgacct tattttagat agaatgacag tatagaatgg
    19501 aaggaaatca tgtctacaaa tagaaaaaat gatatgatgg tttattgctc attttgtggc
    19561 aaaaaccaag aagaagtaca aaaaataatt gctggcaaca atgcttttat ttgtaatgaa
    19621 tgcgtggagt tagctcagga aatcattcga gaagaattgg ttgaggaagt cttggcagac
    19681 ttgtctgagg tgccaaaacc aattgaactc ctccatatct tgaaccacta tgtaattggt
    19741 caagatcgtg ccaagcgtgc cttggcagtg gcggtttata accactacaa acgcatcaat
    19801 ttccacgata cacgcgaaga gtcagaagat gtggatttgc agaagtcaaa cattttgatg
    19861 attggcccaa ctggttcagg gaaaactttc cttgcccaga ccttggctaa gagcttgaat
    19921 gtaccttttg ctattgcgga tgcgacagct ctgacggagg ctggttatgt gggtgaggat
    19981 gtggaaaata tactcctcaa actcttgcag gttgctgact ttaacatcga acgtgcagag
    20041 cgtggcatta tctatgtgga tgaaattgac aagattgcca agaagagtga gaatgtgtct
    20101 atcacacgtg atgtttctgg tgaaggggtg caacaagccc ttctcaagat tattgaggga
    20161 actgttgcta gcgtaccgcc tcaaggtgga cgcaaacatc cacaacaaga gatgattcaa
    20221 gtggatacaa aaaatatcct cttcatcgtg ggtggtgctt ttgatggtat tgaagaaatt
    20281 gtcaaacaac gtctgggtga aaaagtcatc ggatttggtc aaaacaataa ggcgattgac
    20341 gaaaacagct catacatgca agaaatcatc gctgaagaca ttcaaaaatt tggtattatc
    20401 cctgagttga ttggacgctt gcctgttttt gcggctcttg agcaattgac cgttgatgac
    20461 ttggttcgca tcttgaaaga gccaagaaat gccttggtga aacaatacca aaccttgctt
    20521 tcttatgatg atgttgagtt ggaatttgac gacgaagccc ttcaagagat tgctaataaa
    20581 gcaatcgaac ggaagacagg ggcgcgtgga cttcgctcca tcatcgaaga aaccatgcta
    20641 gatgttatgt ttgaggtgcc gagtcaggaa aatgtgaaat tggttcgtat cactaaagaa
    20701 actgtcgatg gaacggataa accgatccta gaaacagcct agaggtgact atggaactta
    20761 atacacacaa tgctgaaatc ttgctcagtg cagctaataa gtcccactat ccgcaggatg
    20821 aactgccaga gattgcccta gcagggcgtt caaatgttgg taaatccagc tttatcaaca
    20881 ctatgttgaa ccgtaagaat ctcgcccgta catcaggaaa acctggtaaa acccagctcc
    20941 tgaacttttt taacattgat gacaagatgc gctttgtgga tgtgcctggt tatggctatg
    21001 ctcgtgtttc taaaaaggaa cgtgaaaagt gggggtgcat gattgaggag tacttaacga
    21061 ctcgggaaaa tctccgtgcg gttgtcagtc tagttgacct tcgtcatgac ccgtcagcag
    21121 atgatgtgca gatgtacgaa tttctcaagt attatgagat tccagtcatc attgtggcga
    21181 ccaaggcgga caagattcct cgtggtaaat ggaacaagca tgaatcagca atcaaaaaga
    21241 aattaaactt tgacccaagt gacgatttca tcctcttttc atctgtcagc aaggcaggga
    21301 tggatgaggc ttgggatgca atcttagaaa aattgtgagg aaaagaaaat ggcaaaaaca
    21361 attcatacag ataaggcccc aaaggctatc ggaccctatg ttcaaggaaa aatcgttggc
    21421 aaccttttgt ttgctagcgg tcaagttccc ctatcccctg aaactgggga aattgtagga
    21481 gagaatatcc aagaacagac agagcaagtc ttgaaaaaca tcggtgctat tttggcagaa
    21541 gcaggaacag actttgacca tgttgtcaaa acaacttgct tcttgagcga tatgaacgac
    21601 tttgttcctt ttaatgaggt ttaccaaacg gccttcaaag aggaattccc agctcgttca
    21661 gctgtggagg tagctcgtct tcctcgtgat gtaaaagtcg aaattgaagt catcgcagag
    21721 attggataag ctagttgaag tttggtgttg ccaaacttct tttgatataa ggagaaaaag
    21781 atgacaaaga aacaacttca cttggtgatt gtgacaggga tgagtggcgc agggaaaact
    21841 gtagccattc agtccttcga ggatctaggt tatttcacca ttgataatat gccgccagct
    21901 ctcttgccta agtttttgca gctggttgaa attaaggaag ataatcctaa gttggctttg
    21961 gtagtggata tgcgtagccg ttctttcttt tcagagattc aagctgtttt ggatgagttg
    22021 gaaaatcaag atggtttgga tttcaaaatc ctctttttgg atgcggctga taaggaattg
    22081 gtcgctcgtt acaaggaaac cagacggagt cacccactag cagcagacgg tcgtatttta
    22141 gatggaatca agttggaacg tgaactcttg gcacctttga aaaatatgag ccaaaatgtg
    22201 gtggatacga ctgaactcac tccacgtgaa ctgcgcaaaa cccttgcaga gcagttttca
    22261 gaccaagaac aagcccagtc tttccgtatc gaagtcatgt ctttcggatt taagtatgga
    22321 atcccgattg atgcggactt ggtctttgat gtccgtttct tgccaaatcc ctattatcta
    22381 ccagaactga gaaaccaaac gggtgtggat gaacctgttt atgattatgt catgaaccat
    22441 cctgagtcag aagactttta tcaacattta ttggccttga ttgagccgat tctgccaagt
    22501 taccaaaagg aaggtaagtc cgttttgacc attgctatgg gatgtacggg tggacaacac
    22561 cgtagtgtgg catttgctaa acgcttggcg caggacttat ccaagaattg gtctgttaat
    22621 gaagggcatc gcgacaaaga ccgcagaaag gaaacggtaa accgttcatg agaaaaccaa
    22681 agataacggt gattggtgga gggactggaa ttcccgtcat tctaaaaagt ctgcgggaaa
    22741 aagatgtgga aatcgcagct atcgtgacgg tggcagatga tggtggttct tcaggtgaac
    22801 tccgaaaaaa tatgcaacag ttgacaccgc caggtgatct tcgtaatgtc cttgtggcca
    22861 tgtcggatat gcctaagttt tatgagaagg tctttcagta tcggttctct gaggatgccg
    22921 gagcctttgc tggccatcca ttgggaaatc tcatcattgc tggcttgtca gaaatgcagg
    22981 gttcaaccta taatgccatg cagttattga gcaaattttt ccatacaaca gggaaaattt
    23041 atccttccag tgaccatcct ttgacccttc atgcagtctt tcaggatggg acagaagtgg
    23101 ctggagagag tcatattgta gaccatcgag gcataattga caatgtctat gtgaccaatg
    23161 ccctaaacga tgatacgcct ctggccagcc gtcgagtagt gcagaccatc cttgaaagtg
    23221 acatgattgt cctagggcca ggttccctct ttacctctat tttgcccaat atcgtgatta
    23281 aggaaattgg gcgggctctt ttggaaacta aggcagaaat tgcctatgtc tgcaatatca
    23341 tgacccaacg tggggagacg gaacacttta cagatagcga ccacgtggaa gtcttgcatc
    23401 gtcaccttgg tcgccctttt atcgacactg tcttggtgaa tattgaaaaa gtgcctcagg
    23461 aatacatgaa ttccaaccgt tttgatgaat acttggtgca agtggaacac gattttgtag
    23521 gtctttgtaa gcaagtttcg cgcgtgattt catctaactt ccttcgtctg gaaaatggcg
    23581 gtgccttcca cgatggagat ttgattgtgg acgagttgat gcgcattata caggtgaaaa
    23641 aatgagtttc acagtagcag taaaagaaga aatcctgggc caacaccatc tgagccggca
    23701 tgaattatct gccattatca aaatgtctgg tagcatcggt ctctcgactt cgggcttgac
    23761 tttgtcagtt gtgacagaaa atgccaaact ggcccgtcac ctctatgagt cctttctcca
    23821 tttctatgaa atcaaatctg aaattcgtca ccaccaacgg agcaatcttc gcaagaatcg
    23881 cgtctatacc gtttttacag atgaaaaggt gcaggatttg ttaagtgatt tgcacttggc
    23941 agactctttc tttggcctgg aaacaggtat tgatgaggcg attttatcgg atgaggaagc
    24001 aggtcgtgcc tatctctgtg gcgctttctt ggcaaatggg agcattcgtg accctgagtc
    24061 aggcaagtac cagttggaaa tcagttctgt ttatctggac cacgcgcaag gaattgcctc
    24121 ccttctccaa cagtttttac tggatgccaa ggtgcttgag cgcaagaagg gggctgtgac
    24181 ctatctccag cgagcagaag acattatgga cttcttgata gtaatcggag ccatgcaggc
    24241 acgtgatgat tttgagcggg ttaagatttt gcgagaaacc cgtaacgacc tcaatcgggc
    24301 taataatgcc gagacagcta atatcgctcg gacagtttct gccagcatga agaccattaa
    24361 caatatcagt aaaataaaag atatcatggg cttagaaaat ttgccagtag atttgcagga
    24421 agtggcgcaa ctgaggattc agcacccaga ctactctatc cagcagttag cagatagtct
    24481 cagcacccct ctgaccaaaa gtggtgtcaa tcacagactc agaaaaatca ataagatagc
    24541 ggatgaatta taaaaccacg aatcctatgt gactcgtggt tcttttttat aaactggtag
    24601 agtgttttgg ttgtactttt tgttcagggt caatgtagtt gatagcgttg ttgacagcag
    24661 ttggagcttc tccgaggcct gtcgcaatca gatcaatttt tccgtcatag tagcagcagt
    24721 caccgatagc atagatacct gcttggctgg attcctgttt gctgttgacg ataatcttgt
    24781 gacggttgag gtcgagcccc cagtttttaa ggttaccgac agaagatttg aaaccatagt
    24841 tgacaaagag gtggtctagg tcaatggttt cagtttcatc agatttgact tttgtgattt
    24901 caagtttatc aagtgttttt ccatttccaa ggagttggct aggggcgaat ggtgtcttga
    24961 tggttacaga tgattcttgc aaggcttgaa cactgtgttc caaggcacgg aaattatctc
    25021 tgcggtgaac aagggtagtt ggtgcgattt tttcaaaagc caaagtccaa tccacagccg
    25081 agtctccccc accaagaatc gtcacttttt taccagcgta ttgctgaatg ttagaaacgt
    25141 ggtagtggat attttcatag ccctcaaccc cttcaagttc cagcggacgt ggtttgaagg
    25201 caccgccacc catagcgatg ataactgttt tagtcaggtg acttccttta gaagttgtga
    25261 tggcaaattc ttcttcttgt ttgtcaatct caagaaccgt ttcattgaga tgaatagggg
    25321 tatcaaatcc atttagctgt tcaatcaagc ggttagtcaa ctcttctcca gtcaggtttg
    25381 ggaagcctgg tacgtctagg atttcctttt cagggtagag aatagcaggt tgtccaccta
    25441 gctggggaag agagtcgatg atttgaacct tggcttggcg taggtgggca taaaaggctg
    25501 caaaaagccc gacaggacca ccacccacaa tggtaatatc atagagttga gacatagttt
    25561 ctcctttgtt ttttctagtc agtttatttt atcatatttt tgttcaatgt aaaatggact
    25621 aggataggga aaaaaatgcc agaaaatggt ataataggac gaaagtgttt tgatagggag
    25681 gatatcaggg atgaattttc aacaattatc caatttgcaa tattggacca gtttgtttgc
    25741 aagtccatgg acgatagcta tcaatctgat tgatattttg attgttgctt atattttata
    25801 ccattttaca aaagctattg caggaaccaa gattatgatt ttggtacgtg gagttttggt
    25861 atttatttta gctcaaatcc ttgcaaatat gattggtttg actacgattt cttggttaat
    25921 caatcaaatt attacttatg gggttattgc ggcggttgtt atcttctctc cagagattcg
    25981 gactggtttg gaacgtttgg gaagagcgac agatttcttt tccaatgccc ctattagtgc
    26041 tgaggaacag atgattcgtg cctttgttaa gtctgttgaa tacatgagtc ctcgtaaaat
    26101 cggggccttg gttgctattc agcgtgtacg taccttgcag gagtatattt cgacaggaat
    26161 tcccttggat gctaagattt ctgcagaact tctcattaac atttttattc ccaacactcc
    26221 cctacatgac ggtgcggtga ttatcaaaga agaacgtatc gctgtgacgt ctgcctatct
    26281 gcccttgaca aaaaacacag ggatttccaa ggaatttggg accagacacc gggcggctat
    26341 cggtttatca gaagtctcag atgccttgac ttttgtcgta tcagaggaaa cgggaggaat
    26401 ttcgataacc tataatggaa ggtttaagca caacctaaca cttgatgaat ttgaaacaga
    26461 attacgtgaa atcttacttc caaaagagga agtaggtctt agttttaaag aacgattgct
    26521 aggaggatgg aaacatgaaa aaaaatagtt tatatatcat atcctcactc ttttttgctt
    26581 gtgtcttatt tgtctatgct acggcgacga attttcaaaa cagtaccagt gctaggcagg
    26641 taaaaacgga aacctatact aatacagtaa caaatgtccc tattgacata cgctataata
    26701 gtgataagta ttttattagc ggttttgctt cagaagtatc agtggtcttg actggtgcaa
    26761 atcgcctatc gctagctagt gaaatgcaag aaagtacacg taaattcaag gttactgctg
    26821 acctaacaga tgccggtgtt ggaacaattg aagttccttt gagcattgaa gatttaccca
    26881 atgggctgac cgctgtggcg actccgcaaa aaattacagt caagattggt aagaaggctc
    26941 agaaggataa ggtaaagatt gtaccagaga ttgaccctag tcaaattgat agtcgggtac
    27001 aaattgaaaa tgtcatggtg tcagataaag aagtgtcgat tacgagtgac caagagacat
    27061 tggatagaat tgataagatt atcgctgtct tgccaactag cgaacgcata acaggtaatt
    27121 acagtggttc agtacctttg caggcaatcg atcgcaatgg tgttgtctta ccggcagtta
    27181 tcactccgtt tgatacaata atgaaggtga ctacaaaacc agtagcacca agttcaagca
    27241 catcaaattc aagtacaagc agttcatcgg agacatcttc gtcaacgaaa gcaactagtt
    27301 caaaaacgaa ttaaaaatag aaaaggattt tataacaatg ggtaaatatt ttgggactga
    27361 tggagtccgt ggagaagcta acctagaact aacaccagaa ttagccttta aactaggacg
    27421 ttttggaggc tatgttctta gtcaacatga aacggaagcg ccgaaagtct ttgtaggacg
    27481 tgacacacgt atttcagggg aaatgctaga atcggccttg gtggcaggtc tcctttcagt
    27541 agggattcac gtatacaaac ttggtgtcct tgcaacacca gcagtagctt acttggttga
    27601 aactgaagga gcaagtgctg gtgtcatgat ttctgctagc cacaatccag ctcttgataa
    27661 cggaatcaag ttctttggcg gtgatggctt caaactagat gatgaaaaag aagcagaaat
    27721 tgaagccttg ctagatgctg aggaagacac tcttcctcgt ccaagtgcag aaggcttagg
    27781 aattttggta gattatccag aaggcttgcg taagtatgaa ggataccttg tttcaactgg
    27841 aactcctctt gatggaatga aggttgcctt ggatacagct aatggagcag cttctaccag
    27901 tgcccgtcaa atctttgcag accttggtgc ccaattgacg gttatcgggg aaacaccaga
    27961 cggtcttaac atcaacctta atgttggttc aacacatcca gaagcccttc aagaagtggt
    28021 caaagaaagt gggtcagcta ttggtttggc ctttgatgga gacagtgacc gcttgattgc
    28081 tgttgatgag aatggtgaca tcgtcgatgg tgacaagatt atgtacatca tcggaaaata
    28141 cctttctgaa aaaggacaat tggctcaaaa tacaattgtg acaactgtta tgtctaacct
    28201 tggtttccac aaggccttga atcgcgaagg tattaacaag gcagttactg cagttggtga
    28261 ccgctacgtt gttgaagaaa tgagaaaatc aggttacaac cttggtggtg aacagtctgg
    28321 tcacgttatc ttgatggatt acaataccac aggtgatggt caattatcag caattcaatt
    28381 gactaaaatc atgaaggaaa ctggtaagag cttatcagag ttggcggcag aagtaacgat
    28441 ttatccacaa aaattagtta atatccgagt ggaaaacgtc atgaaggaaa aggccatgga
    28501 agtgccagct atcaaggcca tcatcgagaa gatggaagaa gaaatggcgg ggaacggccg
    28561 tatccttgtt cgtccaagtg gaacagagcc cctcttgcgt gttatggcag aagcgcctac
    28621 aacagaagaa gtggactact atgttgatac tatcacagat gtagttcgtg ctgaaattgg
    28681 gattgactaa atcctagaaa actagatgaa aataaaaaat tatggtacaa tagcttataa
    28741 tattgtagcc gatggagaaa gacatgaatc ttaaacgtga acaagaattt gttagtcagt
    28801 atcattttga tgcacgtaat tttgaatggg aaaatgaaaa tggagctcct gaaaccaagg
    28861 tggatgtgaa tttccaattg ctccaacatg accaagaaaa tcaagtgact tccttgattg
    28921 tcattttgag ctttatgatt gtatttgata agtttgtcat tagtggaaca atctcacaag
    28981 taaaccatat cgatggccgt attgtcaatg aaccaagtga attgaaccaa gaagaggtgg
    29041 aaaccttggc tcgtccatgt ttgaacatgc tcaatcgttt gacttacgaa gtgactgaaa
    29101 ttgcattaga cttaccagga attaatttgg agttctaata tgaaattagc tgttttcaca
    29161 gattcctctg cttatctcag tgaagagacc ttgcaaagag aagacttgtt tgtcttggat
    29221 attcctgtca atattgatgg tgaggaatat gtcgaaggca tcaatctgtc tgctgaggaa
    29281 ttttaccaaa aaatggctca ggcttctgaa ttgcctaaga ccagtcaacc aagtattgcc
    29341 aagttagatg agatcttaac ttcgctcaaa gaacaaggct atacacatgc cttggggctt
    29401 ttcctatctt ctggaatttc aggtttttac caaaatatcc agtatatggt cgatgactat
    29461 gagggcttaa ccattgcttt cccagacact ttgattacaa gtgctcccct aggcatcatg
    29521 gttgaaagcg tctttaattg gcgtgatcag ggcgatgatt ttgccagcat tcaggataag
    29581 ctagccattc aaatcagccg tacgtcagcc tttatcatgg tagatgactt ggatcatttg
    29641 gtaaaaggtg gacgcctttc aaatggggct gccattttgg gcaatttgct tagcattaag
    29701 ccaatccttt attttaacga tcaaggtgtg attgaagttt acgaaaaagt tcgtactgaa
    29761 aagaaggcca ccaagcgctt aattgaaatt atcaaggaaa caacggcttc aggtcaatac
    29821 cgggtcattg tcattcatgg gaatgctcct gaaaaggctg aagaattgcg tcagcacttg
    29881 cttgattttg gcttgggttc ggatgtttca cttgctacat ttggtagtgt cattggaacg
    29941 cacctaggag caggaagtat tgctctgggt tatattccag tgatttagtt ggcgcttcta
    30001 ggctagttaa gatgccttgt atcttagaaa ttgaacacgg actacctgcc tcttgaaaaa
    30061 agatgcctgt cttgcctttc gtggaaagtc agtgccattc cctatttttc agaggcagct
    30121 aaggtccttt gtatcttgat aattgaacac gcctggaacc ctgtgtgaaa aagatagttc
    30181 ttccaagaag tagacacttc ttgattggaa ttcctatttt cactttgtgt tcttacggac
    30241 tttgtatctt agacaggagt agagatgagt attcgagtaa ttattgccgg ttttaaggga
    30301 aagatgggcc aggctgcttg tcagatggta ttgactgatc cagacttgga cttggtggca
    30361 gttttggatc cttttgagtc tgagtcagaa tggcagggta ttcctgtttt caaggataag
    30421 gctgatttag ctggttttga agcggatgtc tgggtagatt ttactactcc agctgttgcc
    30481 tacgaaaata cacgttttgc tcttgaaaat ggctttgctc cagtagttgg aacgactggt
    30541 ttcacgagtg aagaaattgc agagctaaaa gaattttctc gtgcccaaga cttgggtggc
    30601 ctgattgccc ctaactttgc cttgggtgct gtcttactca tgcaatttgc gacgcaggct
    30661 gccaaatatt tcccaaatgt ggagattatt gagctccatc atgacaagaa aaaggatgct
    30721 ccgagtggaa cagccattaa aacagctgag ttgatggcag aggttcgaga gtccattcag
    30781 caaggcgcag cagatgagga agagctgatt gctggtgctc gtggtgctga ctttgatgat
    30841 atgcgcatcc actcagttcg tttgccaggc ttggtagctc atcaggaagt catctttggc
    30901 aatcagggag aagggttgac cctccgtcat gactcctatg atcgcatctc cttcatgaca
    30961 ggagtcaatt tgggaattaa agaagttgtc aagcgtcatg agcttgtcta tggattagaa
    31021 cacttattat gagattaacg caaatgcctt ctgaatttca gaaggcttta ccagtattag
    31081 aaaaaattaa agaagcaggc tttgaggctt attttgttgg gggctctgtt cgagatgccc
    31141 ttctccatag ccctatccac gatgtggata ttgcgacgtc ttcttatcca gaagagacca
    31201 agcagatttt tccgcgaaca gccgatatcg gaatcgagca tggaaccgtc ttggtcttag
    31261 atggggatga ggagtatgag gtaacaacct ttcggacaga ggatgtctat gtggactatc
    31321 gcagacccag tgcggtttcc tttgtacgat cgctagaaga agacctcaaa cgccgtgatt
    31381 tcacagtcaa cgcctttgcc ttggatgaga caggagaaat cgttgacttg ttccatggtt
    31441 tagaagattt ggaaaagcaa gtcttgcgag cagttggagt ggctagtgag cgtttcaacg
    31501 aagatgcttt acggattatg cgtggtttcc gttttcaggc tagtcttggt tttgcacttg
    31561 agccagaaac atttaaagct atgaagacct tgacgccgct tttggagaaa atttctgtag
    31621 agcgtacctt cgtcgagttt gataaactct tgctggctcc attttggaga aggggcttgg
    31681 cttccatgat tgagagtcaa gcttatgact atctccctga tatggcatct agccaggaca
    31741 agctcaacag actgtttgat ttggagactg attttacttt tgaatcctct gaacaagcct
    31801 gggcggcttt actatgggct ttggagattg aaaatgcgca gtcatttttg aaatcttgga
    31861 agacctcacg ccagtttgcc aagcaagttc aggatttgct gattattttg gctctgcgtg
    31921 aaaatggaga attgagtaag cgagattgtt atcgctttga catagatttg cttttacagg
    31981 ctgaaaatct tcgtcaagct caaggaaaag aagtcaaccc acaagccatc acagaaaaat
    32041 accaaagctt gactatccat gacaagaaag agattcagat taatggcgat attttgatca
    32101 aggaatatgg ctatcagcca ggcccagact tgggagagat tttaacagag attgagtttg
    32161 ccattgtcga tggagaattg gagaataatc gtgaagccat ccatgcttac ctgagggaga
    32221 aaaaatgagt gattttatcg ttgaaaaact aagtaaatcc gttggtgaca agaccgtttt
    32281 tagggatatt tcctttatta tccatgactt agacagaatt ggtttaatcg gtgtcaatgg
    32341 gactggcaag accacccttt tggacgtcct ttctggtgtt tctggatttg atggggatgt
    32401 cagtcctttt tcagctaaaa atgattacca gattggttac ttgactcagg atcctgattt
    32461 tgatgataga aagacagttt tggatacggt tctatctagt gaactcaagg aaatccagct
    32521 cattcgtgag tatgaattga ttatgctcga ctatagtgag gacaagcagg cgcgtttgga
    32581 acgtgtcatg gcagagatgg actctctcca agcttgggaa atcgaaagtc aggtcaagac
    32641 cgttcttagc aaattgggca ttcaagactt atctactcct gttggggaat tgtcaggtgg
    32701 tctgagaaga cgggtacagt tggcacaagt cttacttggc aaccacgacc tcttgctttt
    32761 ggatgagccg accaaccatc tggatattgc gattattgag tggctgaccc tctttttgaa
    32821 aaattctaag aagaccgtcc tttttatcac tcacgatcgt tatttcttag acgctttgtc
    32881 aacacggatt ttcgagttgg atcgtgcagg cttgaccgag taccagggca attaccagga
    32941 ctatgttcgc ctaaaggcgg aacaggatga gcgcgacgcg gctcttcttc acaaaaaaga
    33001 acaactctac aaacaagaac tggcctggat gcgcagacaa ccgcaggcgc gtgcgaccaa
    33061 gcagcaggcc cgtatcaatc gattccatga tctgaaaaag gaagtttcag gcagtagtgc
    33121 tgagacagac ttgaccatga actttgaaac cagtcggatt gggaagaaag tcatcgagtt
    33181 tcaggctgtt tcctttgcct atgaaaacaa gcctattttg caagatttta atctcttggt
    33241 gcaggccaaa gaccgtatcg gaatcgttgg ggacaatggt gttggaaaat caaccctgct
    33301 caacttgatt gcaggaagtc ttgagccaac taaaggacaa gttgtgattg gggaaactgt
    33361 tcgcatcgcc tatttctctc agcaaattga gggtttggat gaaagcaaga gagttatcaa
    33421 ttacctgcag gaagtggcag aagaggtcaa gaccagtggc ggttctacga cttcgattgc
    33481 agagttgctg gagcagttcc ttttcccacg ttcgacgcat ggaaccttga ttgagaaatt
    33541 gtcagggggt gagaaaaaac gtctttatct cctcaaactg cttttggaaa aaccaaatgt
    33601 tcttctttta gacgagccaa ccaatgacct agatattgca actttgacag tcttagagaa
    33661 tttcttgcaa ggttttgcag gtcccgtttt aacagtcagt cacgaccgct atttcttgga
    33721 taaggtagcg accaagattc tcgcttttga ggatagcaag attcgtcctt tctttggtca
    33781 ttacaccgac tatcttgatg aaaaagcttt tgaaacagat atggccaatc aagtgcaaaa
    33841 ggtcgaaaag gaaaaagtgg tcaaggttcg agaagacaag aaacgtatga cctaccaaga
    33901 aaagcaggag tgggcaagta ttgaaggtga tattgaaacc ttggaaaaac gtatcgctgc
    33961 tattgaagag gaaatgcagg ctaacggctc tgactttggt aagctggcta ctctccaaaa
    34021 agaattggat gagaaaaatg aagcactcct tgaaaaatac gaacgctatg agtatctcag
    34081 tgaatttgat agttaagact attcgaaaat caaagtgcaa actatgtcgg cttcacattg
    34141 ccgactcaag tacagcctgt gaccggcttc ctaatttgca ctttgatttt tattgagtat
    34201 aaaagaatag aaaaatcccg tctcatgaaa ggatttctct attctttttt ggtttcttga
    34261 tgaaagatat tttgccaggt ttcgtggcga cgccagatac tggtatggat gataccatct
    34321 aactcatagc agatgagttt ggttttttga ctgatggaag taatttgaat gtccttgata
    34381 gcagaattta actctttttt ggtcttataa gcctctttat ccatctgctc tccatcttgg
    34441 cgaatataga caaaatcatc agtcaagagt tcttctagtt ggtttccttg gtcaatcaat
    34501 tgttcacgca taagcaattt tttatagaca ttgtctaaaa tttcatcctc agagataggt
    34561 gctggttcga tatggacatc ggtatcaaag acgccaaaac gctcctccag catagactcg
    34621 acctgatccg cgatttcatg gctttcaaaa acagacaagt caggattcat ctctagtgta
    34681 atatccaggt agatgttgct accgtaggtg cgacctcttt gcgatttgac cttgctgatt
    34741 ttgggaattt ccatgatagc cttttggtag tcctcgagca ggcggtcgtc aaagccatct
    34801 gaaagactaa aggaagactc gatgaagata tcataggcag tcttcaagat aaagaaagtg
    34861 atgatgatag caaccagttt atccacaatc ggataattga aactactagc taggatggca
    34921 atggcggttc caagtgaggt aacagcgtca gaaagattgt ccttagcagc tgccttcagc
    34981 gccttggagt tggatttctt actgaggcga gtattgtaga gatagaccac aaacataatc
    35041 gctgcagaaa tgattcctag agttgcacca agaggatcaa tgaccgtttc ttcccgactg
    35101 agaatctttt gaatggtatc tcttagaaca tcgaaaccga catagaacat gatgatagaa
    35161 gtgatcaagc ttgccaaatc ttcaatcttc caatgaccaa aacggtggtc acggtctgca
    35221 ggctggcgcg ccatccgaat cccgattaag agggccacat ttccaatgat gtccgatacg
    35281 ttattaaaac catcggccac caaactggat gaatgaagga gatgaccagc tgctaatttg
    35341 gctgcagaca agatcaaata ggtcgaaata ctgataatgg ctccacgctc agctaacttg
    35401 agatttgaga tagattgctt cattcaactt cctttctagt cacatagcat tctaccatta
    35461 tactggtttt caaggaaaaa ttcaaataag gcagttttta ctctttgaaa actccccaaa
    35521 atttggtata atggtactac tcaagggagt agctggcaga aacctgtgat agtgtcgtca
    35581 ttccgaattt tatgctgaaa agtatgcttt ccggccctat cttaaacagc gagacttgtt
    35641 atgattaaca ggtctctttt tgtttgtcgt gaaaagatat gacagaaaga gagtctgttt
    35701 tgcacacaat gtcaaggagg agacacatgt caaaagaaca aaaacgccaa gcgttttata
    35761 ctcagagccc tgaagaggtc ttgcaggctg tggatgcgac cgagcaaggt ttgtcatcaa
    35821 gtgaggcgga aaagcgcctt gccgaatttg ggcacaatga actcgaagaa ggcgagaaac
    35881 gatcaatctt ggtcaaattc atcgagcaat ttaaggattt gatgattatc atcctagttg
    35941 cggcagcaat cttgtcagtc gtgacttctg gtggggaaga tatcgcagat gccattatca
    36001 tcctagctgt ggttatcatc aacgctgcct ttggtgttta ccaagaagga aaagctgaag
    36061 aagctattga agccctcaaa tccatgtcta gtccagttgc ccgcgttctt cgtgatggac
    36121 acatggcaga gattgactct aaagaattgg taccaggcga tatcgttgcc cttgaagcag
    36181 gtgacgtggt accagcggac ctacgtttga tagaagccaa ctctcttaaa attgaagaag
    36241 cagccttgac aggtgaatct gtaccagtcg aaaaagactt gtcagtcgag cttgcgacag
    36301 atgctggtat tggtgaccgt gtcaacatgg ccttccaaaa ctcaaacgta acctatggtc
    36361 gtgggatggg tgttgttgtc aatacaggta tgtacactga agttggtcat attgctggta
    36421 tgcttcaaga tgcggatgag actgatacac cactcaaaca aaatttgaac aacctttcta
    36481 aggtcttgac ctatgctatc ttggtcattg cccttgttac ttttgtagtg ggtgtcttca
    36541 ttcaagggaa aaatccactt ggtgagttgt tgacttctgt tgcccttgcc gttgcagcca
    36601 ttccagaagg acttcctgct atcgttacca tcgttctttc tcttggtact caagttttgg
    36661 ccaaacgaca ttccatcgtt cgtaagttgc cagcagttga aacacttggt tcaactgaaa
    36721 tcatcgcttc tgataagact ggtacgctga ctatgaacaa gatgacagtc gaaaaagtct
    36781 tctacgatgc ggttctacat gactcagctg atgatattga actaggtctt gaaatgccac
    36841 tacttcgttc agttgtcttg gccaatgata cgaaaatcga tgtggaaggt aacttgattg
    36901 gtgacccaac cgaaacagcc tttatccaat atgccttgga caagggctat gatgtcaaag
    36961 gtttcttaga gaaatatcct cgtgtagctg aattgccatt tgactctgac cgtaagctca
    37021 tgtcaacagt tcacccattg ccagatggtc gtttccttgt agcagtcaag ggtgcgccag
    37081 accaactctt aaaacgttgt cttcttcgtg ataaggctgg ggatattgct ccgattgatg
    37141 agaaggttac aaatctcatt catacaaaca attctgaaat ggctcatcaa gccttgcgtg
    37201 tccttgcagg tgcttataag attatcgata gtattccaga aaatctcact tctgaagagc
    37261 ttgaaaatga tttaattttt actggtttga ttgggatgat tgaccctgaa cgtcctgaag
    37321 ccgctgaggc tgttcgtgtg gctaaggaag cgggaatccg tccaattatg attacaggtg
    37381 accaccaaga cacagcggaa gccattgcca aacgtttggg aatcattgac gcaaacgata
    37441 cagaaggtca cgttttaact ggtgctgaac tcaatgaact gtcagatgaa gaatttgaaa
    37501 aagtcgttgg tcaatactct gtttatgccc gtgtgtctcc agaacacaag gttcgtatcg
    37561 tcaaggcttg gcaaaaacaa ggtaaagtcg ttgccatgac aggtgacggt gtcaatgacg
    37621 cgccagctct gaaaacagcc gatatcggta tcggtatggg aatcactggt acagaggttt
    37681 ctaagggggc ttctgatatg attcttgcag atgataactt tgcgactatt atcgtcgcag
    37741 tggaagaagg acgtaaggtc ttctcaaaca ttcaaaagac tattcagtac ctactttctg
    37801 ctaatactgc tgaagtatta accatcttcc tatcaacctt gtttggttgg gatgtcttac
    37861 agccggttca tcttttgtgg attaacttgg taacggatac cttcccagct atcgctcttg
    37921 gtgttgaacc tgcggaacct ggtgtcatga atcataaacc acgtggacgc aaggcaagct
    37981 tcttctcagg tggtgttttg agttctatca tttatcaagg tgtactccaa gcagctcttg
    38041 ttatgagtgt ttatggcctt gcgattgctt acccagttca tgtgggtgac aatcatgcta
    38101 ttcatgcaga tgccctaacg atggcctttg caacccttgg tttgattcag ctcttccatg
    38161 cctacaatgt caagtctgtc taccaatcca tcttgacagt tggcccattc aagtctaaga
    38221 cctttaactg gtccatcttg gtatccttta tccttctcat ggcaacaatc gttgtagaac
    38281 cgcttgaagg aatcttccac gtaaccaaac tagacttgtc acaatgggga attgttatgg
    38341 ctggaagctt ctcaatgatt atcatcgtcg aaatcgttaa gtttattcaa cgcaaactcg
    38401 gttttgacaa gaatgcgatt taagggaggt ttcggatgtc agcatatcaa ttaccgaccg
    38461 tatggcagga tgaagctagt aatcaaggag cttttacggg gctaaacaga ccaacagcag
    38521 gtgcccgttt cgaacaaaac ttgccaaaag gagaacaagc ttttcagctt tattcactgg
    38581 gaacaccaaa tggtgtgaag gttactatct tattggaaga attactagaa gctggtttta
    38641 aggaagcggc ttacgacttg tataagattg ctatcatgga tggggatcaa ttcggctcag
    38701 actttgtgaa gctcaatcca aattccaaga ttccagcctt attggaccag tcaggtactg
    38761 aaaacgtaag agtctttgag tctgctcata ttcttcttta ccttgctgag aaatttggag
    38821 cctttttacc aagtaatcct gtggaaaagg tagaagtttt gaattggcta ttctggcaag
    38881 caggtgcagc accttttcta ggtgggggat ttggacattt cttcaattat gctcctgaaa
    38941 aattggaata tcctattaac cgttttacga tggaagtgaa acgccagttg gatttattgg
    39001 ataaggaatt ggctcagaaa ccttatattg caggcaatga ctatacgatt gcagatattg
    39061 ctatctggtc ttggtatgga cagttagttc aaggaaatct ttaccaaggt tctgcaaaat
    39121 tcttggatgc ctcaagttat caaaatctag taaaatgggc agaaaaaatt gccaatcgtc
    39181 cagctgttaa gcgtggcttg gaagtaactt atacagaaat taaatagcaa aaaagtcatc
    39241 ttcggatggc tttttgctac agttgaggga aagggtttgc agttatcggc ttttgtgcta
    39301 taattgctat aatatagtaa aaagcaagag gagtgtgagg aagtggaaaa gaaaattgtg
    39361 aaggatatct tatttttatc tcaagtgtct catccggcaa gtcaggagga cctttatctt
    39421 gccagagatt tgcaggatac actcttagca aatcgtgata cctgtgttgg tctagctgcc
    39481 aatatgattg ggatgcagaa gcgcgtgatt atctttaatc ttggcttggt tcccgtggtc
    39541 atgtttaacc cagtgcttct gtcctttgaa ggatcctatg aggcagaaga aggctgtttg
    39601 tccttggtag gtgtgagatc aactaagcgt tatgaaacca taaggcttgc ctatcgtgac
    39661 agcaagtggc aggaacagac cattaccttg acaggcttcc cagctcagat ttgccagcat
    39721 gagctggatc acttggaagg acgaatcatt taggaggaaa gcaaatgaaa cgaatagtct
    39781 ttgaacttat ttttatcgca acgacctggt atatcttttt accgcccctt aacctgacca
    39841 gctgggaatt tctcttcttc ctctgtgggc atttgttagt tgtggcaatc ttatttggct
    39901 ttggcaaggg gataaacctt gtcaaaacgg ttcatgtgcg ccacggtaag gcggaagctg
    39961 ccttaaatct tgagggtttc aaaatcaatc ggttagggaa aattctgtta gcttcgattg
    40021 gaggaattct tctcttggca gctttggttt ccttggtaac ttctagcatg tttcaggcta
    40081 aaaattatgc caatgtagtc acggttacgg aaaaagactt tactgaattt cctaagagtg
    40141 acaccagtaa ggttcctatc ctagatagaa gtactgctga aaaaattgga gaccgctact
    40201 tgggttccct aaccgataag gtgtcgcaat acgtagcggc agatacctat acccaattga
    40261 caattgatgg gaaaccttat cgggtcacac cactagaata tgcagaccct atcaaatggt
    40321 ttaacaatca agccaaggga atcggtgagt atatcaaggt ggacatggta actggaaatg
    40381 cggatttggt ggacttgaag acaccaatca agtattcaga ctcggagtat tttaaccgtg
    40441 atgtcaaacg tcacctgcgc ttgaagtacc cgaccaaaat ctttaaaact ccatcttttg
    40501 aggtggacga tgagggcaat cctttctatg tagcaacggt ttaccaaaag caatttggac
    40561 ttgctgttcc tcgtcctgct tcagtcatta tcttggatgc tacaaatgga gaaaccaagg
    40621 aatacagctt atcagatgtt ccagaatggg tggacaggat ctatccagca gaggaaacca
    40681 ttgagcaaat caactacaac ggcaagtaca aggacggttt cttgaatgcc atgatttcca
    40741 agaaaaacgt gacccagacg accaatggct ataattactt gtctatcggt aatgacatct
    40801 atctctacac aggtgtgacg tcggctaatg cggatgagag taatcttggt ttcatccttg
    40861 aaaatatgcg aacaggagaa attactaagt atagcttggc ttctgcgaca gaagaatcag
    40921 cccgtgaatc agcagaaggt gctgttcagg agaaatccta caaagcaacc ttcccaatcc
    40981 tcatcaacct caatgacaag cctctctaca tcatgggctt aaaggacaat gctggcttgg
    41041 tcaaagagta cgccctggta gacgcagtcg agtaccaaaa tgttatcgtt gctactacag
    41101 tggaagagat gctcagcaag tatgccaata aaaacgacct tgaaattgac aatgcaacga
    41161 cagaaagcat caagggagta gtagcagacc tcaaatcagc tgttatcaag ggagacactg
    41221 tctacttctt taaagttgat ggcaagatat acaaggtaaa ggcttcagta tccgatgacc
    41281 ttccttacct tgaaaatggt aaaaccttcg aaggtcaagt aggaaaagac aattatctca
    41341 agacctttaa agtacaataa tactcttcga aaatctctag ctctatct
//
LOCUS       DAVBCW010000007        38645 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVBCW010000007 DAVBCW010000000
VERSION     DAVBCW010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 38645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38645
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.007"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            434..886
                     /locus_tag="VQ514_000317"
     CDS             434..886
                     /locus_tag="VQ514_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET2499135.1"
                     /translation="MIITKISHLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            905..2242
                     /locus_tag="VQ514_000318"
     CDS             905..2242
                     /locus_tag="VQ514_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET2499136.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2321..3205
                     /locus_tag="VQ514_000319"
     CDS             2321..3205
                     /locus_tag="VQ514_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2499137.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3205..4038
                     /locus_tag="VQ514_000320"
     CDS             3205..4038
                     /locus_tag="VQ514_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2499138.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAVFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4056..6149
                     /gene="nanB"
                     /locus_tag="VQ514_000321"
     CDS             4056..6149
                     /gene="nanB"
                     /locus_tag="VQ514_000321"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HET2499139.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISSLLLDKLSGESQTVVMKFKADKPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIDEISLFNKAISDQ
                     EVSTIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     NETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLRERHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSKVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            6161..7264
                     /locus_tag="VQ514_000322"
     CDS             6161..7264
                     /locus_tag="VQ514_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET2499140.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            7438..8136
                     /locus_tag="VQ514_000323"
     CDS             7438..8136
                     /locus_tag="VQ514_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HET2499141.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            8155..9672
                     /locus_tag="VQ514_000324"
     CDS             8155..9672
                     /locus_tag="VQ514_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET2499142.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADLAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPFGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            9797..11125
                     /locus_tag="VQ514_000325"
     CDS             9797..11125
                     /locus_tag="VQ514_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2499143.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            11377..12243
                     /locus_tag="VQ514_000326"
     CDS             11377..12243
                     /locus_tag="VQ514_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2499144.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            12259..13098
                     /locus_tag="VQ514_000327"
     CDS             12259..13098
                     /locus_tag="VQ514_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2499145.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            13370..13822
                     /locus_tag="VQ514_000328"
     CDS             13370..13822
                     /locus_tag="VQ514_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET2499146.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQAENDQFEYHKNYADLHLLVEGHEYSSYGSRIKDEAVAFDEASDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPNGYAGMEEKVRKYLFKILID"
     gene            13919..14263
                     /locus_tag="VQ514_000329"
     CDS             13919..14263
                     /locus_tag="VQ514_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499147.1"
                     /translation="MVSSKHAARASFLDGQGITVDETSWIINGIVNALIGRYIKLGTY
                     AAKYGISIARSILSRAAATAAARVGLLTKISGWILRVAVNVADVYGNFANNIAAAWDA
                     YDKIPNNGRINF"
     gene            14273..14464
                     /locus_tag="VQ514_000330"
     CDS             14273..14464
                     /locus_tag="VQ514_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499148.1"
                     /translation="MKALCIFLLNMLAWTVLAMIIRGGLDGFDEHTWSTILIASLFGV
                     YDYKPIDKNRKKSKRKKKK"
     gene            14547..15191
                     /locus_tag="VQ514_000331"
     CDS             14547..15191
                     /locus_tag="VQ514_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HET2499149.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVISCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLSWKEILQKGLMLASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            15360..16277
                     /locus_tag="VQ514_000332"
     CDS             15360..16277
                     /locus_tag="VQ514_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HET2499150.1"
                     /translation="MSDLKKYEGVIPAFYACYDDQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGAKAGIGGTYGAMPE
                     LFLKLNQLITDKDLETARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKERFL"
     gene            16295..17179
                     /locus_tag="VQ514_000333"
     CDS             16295..17179
                     /locus_tag="VQ514_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2499151.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(17195..18046)
                     /locus_tag="VQ514_000334"
     CDS             complement(17195..18046)
                     /locus_tag="VQ514_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HET2499152.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKNSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            18246..20303
                     /gene="pbp2b"
                     /locus_tag="VQ514_000335"
     CDS             18246..20303
                     /gene="pbp2b"
                     /locus_tag="VQ514_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HET2499153.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKIVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            20314..20910
                     /gene="recR"
                     /locus_tag="VQ514_000336"
     CDS             20314..20910
                     /gene="recR"
                     /locus_tag="VQ514_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HET2499154.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVATMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            21086..22129
                     /locus_tag="VQ514_000337"
     CDS             21086..22129
                     /locus_tag="VQ514_000337"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HET2499155.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            22213..23586
                     /locus_tag="VQ514_000338"
     CDS             22213..23586
                     /locus_tag="VQ514_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HET2499156.1"
                     /translation="MKLTIHEVAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGVRDGHDFIERAFENGAAATLSEKEVANHPYILVDDVLTAFQQLAAYYLEKTAVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPTDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQVLDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIRLAFQHLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGVQSVQLHNQMILSLSPDVLDTVIFYGEDIAE
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAHDQILLKGSNSMNLAKLVE
                     SLENEDK"
     gene            23573..24184
                     /locus_tag="VQ514_000339"
     CDS             23573..24184
                     /locus_tag="VQ514_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2499157.1"
                     /translation="MKTSDFVKYLQRMIAITDTGLTFTKDPFDRERYEDLRDLLSEML
                     NQASDLDSEEVAEVLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEDSWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKLLDGQFQEN
                     QEIADLQFFAIDPLPNLSEKRITKEQIEILWQVYQGQREQYLD"
     gene            24275..24979
                     /locus_tag="VQ514_000340"
     CDS             24275..24979
                     /locus_tag="VQ514_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HET2499158.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            25198..26571
                     /gene="ftsA"
                     /locus_tag="VQ514_000341"
     CDS             25198..26571
                     /gene="ftsA"
                     /locus_tag="VQ514_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HET2499159.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENIIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVESVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            26588..27847
                     /gene="ftsZ"
                     /locus_tag="VQ514_000342"
     CDS             26588..27847
                     /gene="ftsZ"
                     /locus_tag="VQ514_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HET2499160.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            27852..28523
                     /locus_tag="VQ514_000343"
     CDS             27852..28523
                     /locus_tag="VQ514_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HET2499161.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKATQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            28533..29072
                     /locus_tag="VQ514_000344"
     CDS             28533..29072
                     /locus_tag="VQ514_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HET2499162.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTPVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            29072..29335
                     /locus_tag="VQ514_000345"
     CDS             29072..29335
                     /locus_tag="VQ514_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HET2499163.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            29332..30117
                     /locus_tag="VQ514_000346"
     CDS             29332..30117
                     /locus_tag="VQ514_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET2499164.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            30126..30914
                     /gene="divIVA"
                     /locus_tag="VQ514_000347"
     CDS             30126..30914
                     /gene="divIVA"
                     /locus_tag="VQ514_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HET2499165.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            31262..34054
                     /locus_tag="VQ514_000348"
     CDS             31262..34054
                     /locus_tag="VQ514_000348"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HET2499166.1"
                     /translation="MKLKDTLNLGKTEFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     QGKPHFTLHDGPPYANGNIHVGHAMNKISKDIIVRSKSMSGFYAPFIPGWDTHGLPIE
                     QVLSKQGVKRKEMDLVEYLKLCREYALSQVDKQREDFKRLGVSGDWENPYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPAGEARKFVVAAELLTSLSEK
                     FGWADVQVLETYRGQELNHIVTEHPWDTAVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKVKFHSEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMVAETIEHVAQLFEEHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVVVNRPELTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFALDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDTVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYANFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFEAEDFVQLSELPEVQTFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SELTIAEEPAPEAALSFEDVVFTVERAAGEVCDRCRRIDPTTAERSYHAVICDHCASI
                     VEENFADAVAEGFEEK"
     gene            34464..34826
                     /locus_tag="VQ514_000349"
     CDS             34464..34826
                     /locus_tag="VQ514_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499167.1"
                     /translation="MKSSGIVGLVGGILLLICPFVLLGTAVNTAATTLNGGATAGAFS
                     GVALLLNALKIANLVLGIIAIVYYKGDKHVGAAPSVLMIVSGEVSLIPFLGWVGGILA
                     IIGGSLFLATLKKFKSEE"
     gene            35361..36053
                     /locus_tag="VQ514_000350"
     CDS             35361..36053
                     /locus_tag="VQ514_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HET2499168.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            36106..38517
                     /locus_tag="VQ514_000351"
     CDS             36106..38517
                     /locus_tag="VQ514_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HET2499169.1"
                     /translation="MIRNKKQDYVLAYKQPASTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALATTSYVYKGTRFE
                     RKAFASFPDDLLIQCFTKEGLETLDFTIELSLTCDLASDGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEADVDASTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLETVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYSGNLFSY
                     KGQEYIEAARASLNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKTSTLPNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
BASE COUNT        11869 a         6813 c         8437 g        11526 t
ORIGIN      
        1 ttagaaaaac gaagtgagca gcttataaat tcaagtcccc aaatagattc atactagtat
       61 cttttgcaaa aaataaaggg cgacttcctt catgaatatc aatttcatct ataaggaagg
      121 tagctaattg aactaactta tttattctgt ttgtcgctag aaaaatcaga cctccttgtg
      181 aagattgagg agatactgaa tgaaaatcaa agaagaaact agcaagctag tagcagattg
      241 cccaaaacac cgccttgagg ttgtagataa gactgaccta tataatccaa ggtgaagcga
      301 ctgtggtttg aagagatttt caaagagtat aggctagaga gtagtgtttt tatgtccttc
      361 tagtagaaaa tgctagacag aagaatgggg aacttggata ggaaaaatag attgagaaag
      421 gaggttagaa gagatgatta ttacaaaaat tagccattta ggaacttatg tgggagtaaa
      481 tccacatttt gcaacattaa tagattttct agaaaaaaca ggactagaaa atttaacaga
      541 aggttcgatt gctatcgatg gtaatcgatt gtttgggaat tgctttactt atctagcaga
      601 tggtcaagca ggggctttct ttgaaacaca ccaaaaatat ttggatattc atttagtttt
      661 ggaaaacgaa gaagccatgg ctgttacatc gccggaaaat gtaagcgtta cccaagaata
      721 tgatgaagag aaagatattg aattatacac agggaaagtg gaacagttgg ttcatttgag
      781 agctggtgaa tgcctcatca cttttccaga agatttacat caacccaagg ttcgtataaa
      841 tgatgaacct gtgaaaaaag ttgtctttaa agttgcgatt tcttaatgta gaaagagaag
      901 aacgatgaaa aaaatgagaa agtttttatg tctagctgga attgcgctag cggctgttgc
      961 cttggtagct tgttcaggaa aaaaagaagc tacaactagt actgaaccac caacagaatt
     1021 atctggtgag attacaatgt ggcactcctt tactcaagga ccccgtttag aaagtattca
     1081 aaaatcagca gatgctttca tgcaaaagca tccaaaaacg aaaatcaaga ttgaaacatt
     1141 ttcttggaat gacttctata ctaaatggac tacaggttta gcaaatggaa atgtgccaga
     1201 tatcagtaca gctcttccta accaagtaat ggaaatggtc aactcagatg ctttggttcc
     1261 gctaaatgat tctatcaagc gtattggaca agataaattt aacgaaactg ccttaaatga
     1321 agcaaaaatc ggagatgatt actactctgt tcctctttat tcacatgcac aagtcatgtg
     1381 ggttagaaca gatttgttaa aagaacataa tattgaggtt cctaaaactt gggatcaact
     1441 ctatgaagct tctaaaaaat tgaaagaagc tggagtttat ggcttgtctg ttccgtttgg
     1501 aacaaatgac ttaatggcaa cacgtttctt gaacttctac gtacgtagtg gtggaggaag
     1561 cctcttaaca aaagatctta aagcagactt gacaagccaa cttgctcaag atggtattaa
     1621 atactgggtt aaattgtata aagaaatctc acctcaagat tctttgaatt ttaatgtcct
     1681 tcaacaagct accttgttct atcaaggaaa aacagcattt gactttaact ctggcttcca
     1741 tatcggagga attaatgcca acagtcctca attgattgat tcgattgatg cttatcctat
     1801 tccaaaaatc aaagagtctg ataaagacca aggaattgaa acctcaaaca ttccaatggt
     1861 tgtttggaaa aattcaaaac atccagaagt tgctaaagca ttcttagaag cactttataa
     1921 tgaagaagac tacgttaaat tccttgattc aactccagta ggtatgttgc caactattaa
     1981 ggggattagc gattctgcag cctataaaga aaatgaaact cgtaagaagt ttaaacatgc
     2041 tgaagaagta attactgaag ctgttaaaaa aggtactgct attggttatg aaaatggacc
     2101 aagtgtacaa gctggtatgt tgactaacca acacattatt gaacaaatgt tccaagatat
     2161 cattacaaat ggaacagatc ctatgaaagc agcaaaagaa gcagaaaaac aattaaacga
     2221 tttatttgag gctgttcagt agatgtaaaa gactagaaaa taggtgggct agtgatctga
     2281 aaagctctag cccaattttg taaaagaagg gagaaggaga atggttaaag aacgtaattt
     2341 aactcgctgg atatttgttt tgccagctat gattatcgta ggattactct ttgtttatcc
     2401 gtttttctcg agtatttttt atagctttac caataagcat ttgattatgc ctaattataa
     2461 atttgttggt ttggctaact ataaagctgt gctatcagat cccaacttct ttaatgcgtt
     2521 ctttaattca attaagtgga ccgttttctc attagttggt caagttttag tagggtttgt
     2581 attggcttta gctcttcaca gagtacgcca cttcaagaaa ttatatagga ccttattgat
     2641 tgttccttgg gcatttccta ccatcgttat tgccttctct tggcagtgga ttctaaacgg
     2701 ggtttatggc tacttaccta atctaatcgt aaaattaggt ttaatggaac atacacctgc
     2761 atttttgaca gatagtacat gggcattcct atgtttggtg tttatcaaca tttggtttgg
     2821 agcaccaatg attatggtta atgtgctttc agctttgcaa acagtaccag aagaacaatt
     2881 tgaggctgct aagatagatg gtgcttcaag ttggcaggtg ttcaagttta tcgtctttcc
     2941 acatattaaa gtggttgtag gacttctagt tgttttgaga actgtatgga tctttaataa
     3001 ctttgacatt atctacctca ttactggtgg tggaccagcc aatgctacaa cgacgcttcc
     3061 aatttttgct tacaacctgg gctggggaac taaattgttg ggtcgtgctt cagcagttac
     3121 agtactgctc tttatcttct tggtggcgat ttgctttatc tactttgcta tcatcagtaa
     3181 gtgggaaaag gagggtagaa aataatgaag aagaaatcca gtatttattt agatattctc
     3241 tcacatgtac ttttagttgg tgcgaccatc gttgcagttt tcccattggt atggattatc
     3301 atatcttctg tcaaagggaa aggggaatta actcagtatc caacacgatt ttggcctgaa
     3361 cagtttacat tagattattt cactcatgtt atcaacgatt tgcacttcat tgataacatt
     3421 cgaaacagtt taatcattgc cttggctaca acccttattg cgattattat ttctgctatg
     3481 gcagcctatg gtattgttcg attctttcct aaattgggag caatcatgtc gagactactc
     3541 gtcattacct acattttccc accaattttg ttagcaattc cctattcaat tgccattgct
     3601 aaagttgggt taacaaatag tttatttggc ttgatgatgg tttatctatc ttttagtgtt
     3661 ccatatgcag tttggctctt agttggattt ttccaaacag ttccaattgg aattgaagaa
     3721 gcggctagaa ttgatggtgc aaataaattt gttacgtttt ataaagttgt gctaccgatt
     3781 gtagcaccag gtattgtagc aacagctatt tatacattta tcaatgcttg gaatgaattc
     3841 ctgtatgcct tgattttgat taacaataca ggaaagatga cagtagcagt agcccttcgt
     3901 tcacttaatg gttcagaaat actagactgg ggagatatga tggcagcgtc tgttattgta
     3961 gttcttccat caattatttt cttctctatc atccaaaata agattgcaag tggattatca
     4021 gaaggatctg tgaagtagac gaaagaagga aaaaaatgaa taaaagaggt ctttattcaa
     4081 aactaggaat ttccgttgta ggcattagtc ttttaatggg agtccccact ttgattcatg
     4141 cgaatgaatt aaactatggt caactgtcca tatctcctat ttttcaagga ggttcatatc
     4201 aactgaacaa taagagtata gatatcagct ctttgttatt agataaattg tctggagaga
     4261 gtcagacagt agtaatgaaa tttaaagcag ataaaccaaa ctctcttcaa gctttgtttg
     4321 gcctatctaa tagtaaagca ggctttaaaa ataattactt ttcaattttc atgagagatt
     4381 ctggtgagat aggtgtagaa ataagagacg cccaaaaggg aataaattat ttattttcca
     4441 gaccagcttc attatgggga aaacataaag gacaggcagt tgaaaataca ctagtatttg
     4501 tatctgattc taaagataaa acatacacaa tgtatgttaa tggaatagaa gtgttctctg
     4561 aaacagttga tacatttttg ccaatttcaa atataaatgg tatagataag gcaacactag
     4621 gagctgttaa tcgtgaaggt aaggaacatt acctcgcaaa aggaagtatt gatgaaatca
     4681 gtctatttaa caaagcaatt agtgatcagg aagtttcaac tattcccttg tcaaatccat
     4741 ttcagttaat tttccaatca ggagattcta ctcaagctaa ctattttaga ataccgacac
     4801 tatatacatt aagtagtgga agagttctat caagtattga tgcacgttat ggtgggactc
     4861 atgattctaa aagtaagatt aatattgcca cttcttatag tgatgataat gggaaaacgt
     4921 ggagtgagcc aatttttgct atgaagttta atgactatga ggagcagtta gtttactggc
     4981 cacgagataa taaattaaag aatagtcaaa ttagtggaag tgcttcattc atagattcat
     5041 ccattgttga agataaaaaa tctgggaaaa cgatattact agctgatgtt atgcctgcgg
     5101 gtattggaaa taataatgca aataaagccg actcaggttt taaagaaata aatggtcatt
     5161 attatttaaa actaaagaag aatggagata acgatttccg ttatacagtt agagaaaatg
     5221 gtgtcgttta taatgaaaca actaataaac ctacaaatta tactataaat gataagtatg
     5281 aagttttgga gggaggaaag tctttaacag tcgaacaata ttcggttgat tttgatagtg
     5341 gctctttaag agaaaggcat aatggaaaac aggttcctat gaatgttttc tacaaagatt
     5401 cgttatttaa agtgactcct actaattata tagcaatgac aactagtcag aatagaggag
     5461 agagttggga acaatttaag ttgttgcctc cgttcttagg agaaaaacat aatggaactt
     5521 acttatgtcc cggacaaggt ttagcattaa aatcaagtaa cagattgatt tttgcaacat
     5581 atactagtgg agaactaacc tatctcattt ctgatgatag tggtcaaaca tggaagaaat
     5641 cctcagcttc aattccgttt aaaaatgcaa cagcagaagc acaaatggtt gaactgagag
     5701 atggtgtgat tagaacattc tttagaacca ctacaggtaa gatagcttat atgactagta
     5761 gagattctgg agaaacatgg tcgaaagttt cgtatattga tggaatccaa caaacttcat
     5821 atggcacaca agtatctgca attaaatact ctcaattaat tgatggaaaa gaagcagtca
     5881 ttttgagtac accaaattct agaagtggcc gtaagggagg tcaattagtt gtcggtttag
     5941 tcaataaaga agatgatagt attgattgga aataccacta tgatattgat ttgccttcgt
     6001 atggttatgc ctattctgcg attacagaat tgccaaatca tcacataggt gtactgtttg
     6061 aaaaatatga ttcgtggtcg agaaatgaat tgcatttaag caatgtagtt cagtatatag
     6121 atttggaaat taatgattta acaaaataaa ggagaaaaac atggttaaat acggtgttgt
     6181 tggagcaggg tattttggag ctgaattggc tcgctacatg caaaagaatg atggagcaga
     6241 gattactctt ctctatgatc cagataatgc agaggcgatt gcagaagaat tgggggcaaa
     6301 agtagcaagt tccttagatg agttggtttc tagcgatgaa gtagattgtg ttatcgtcgc
     6361 aactccaaat aatcttcata aggaaccggt tattaaggct gcacagcatg gtaaaaatgt
     6421 tttctgtgaa aaaccaattg cgctttctta tcaagattgt cgcgagatgg tagatgcgtg
     6481 taaagaaaac aatgtaacct ttatggcagg acatattatg aatttcttta atggtgttca
     6541 tcatgcaaaa gaactcatta atcaaggagt tatcggagac gttctatatt gtcatacagc
     6601 tcgtaatggt tgggaagaac aacaaccgtc agtatcatgg aaaaaaattc gtgaaaaatc
     6661 aggtggtcac ttgtatcacc acatccatga attggattgc gttcaattcc ttatgggggg
     6721 catgcctgaa actgtaacca tgacaggtgg aaatgtggcc catgaaggtg aacatttcgg
     6781 tgatgaagat gatatgattt ttgtcaatat ggaattttct aataagcgtt ttgccttgtt
     6841 agaatggggt tcagcttatc gttggggtga acattatgtc ttaatccaag gaagcaaagg
     6901 tgccatccgc ttagacttat tcaactgtaa aggaactctt aagctagatg ggcaagaaag
     6961 ctatttcttg attcacgaat cgcaagaaga agatgatgat cggactcgta tctatcatag
     7021 tacagagatg gatggagcaa ttgcttatgg taaaccaggt aaacgtactc cattatggct
     7081 atcatctgtc attgataaag aaatgcgcta tctgcatgag attatggaag gagctccagt
     7141 atcagaagaa tttgcaaaac ttttgacagg tgaagctgct ctagaagcaa ttgctactgc
     7201 agatgcttgt acccagtcta tgtttgaaga tcgcaaagta aaattgtcag aaattgtaaa
     7261 ataaattttg gtattctcct atttataggt cgacttgctc ctctgaaagt acttttagag
     7321 gagctgtttg actttgctag tttttgaaac tgaaatctat tatactacaa actattgaaa
     7381 gcgttttaat tttaaggtat aataatctca tagaaataaa gaaaaggagg aaagaggatg
     7441 ccacagatta gcaaagaagc cttgattgag caaatcaaag atggaatcat cgtttcttgt
     7501 caggctcttc ctcatgaacc gctttataca gaagcgggag gggtgattcc cttgctggtc
     7561 aaagcggctg agcaaggtgg agcagtcggt atccgagcaa acagtgttcg cgatatcaag
     7621 gaaattaagg aggtcactaa acttccaatc attgggatta tcaaacgtga ttatccacct
     7681 caggaaccct tcatcacggc tactatgaaa gaagttgatg aattggcaga actggacatc
     7741 gaggtgattg ctctggattg taccaagcgt gaacgctacg atggtttgga aattcaagag
     7801 ttcattcgtc aggttaagga gaaatatcct aatcagcttt tgatggctga tactagtatc
     7861 ttcgaagaag ggctagcagc tgtagaagca ggaattgact ttgtcggaac aaccttatca
     7921 ggctacacat cctacagtcc aaaagtagac ggtccagatt ttgaattgat taagaaactc
     7981 tgtgatgccg gtgtagatgt cattgcagaa ggaaaaattc atacaccaga acaagccaaa
     8041 caaatccttg aatatggagt gcgaggcatc gttgttggtg gcgccattac tagaccaaaa
     8101 gagattacag aacgcttcgt tgctagtctt aaataagatg tgagggggag ttttatgttt
     8161 aaagttttac aaaaagttgg aaaagctttt atgttaccta tagctatact tcctgcagca
     8221 ggtctacttt tggggattgg tggtgcactt tcaaacccaa ccacgatagc aacttatcca
     8281 atactagaca atagtatttt tcaatcaata ttccaagtaa tgagctctgc aggagaggtt
     8341 gtattcagta atttgtcact acttctctgt gtgggattat gtattggctt agcgaaacga
     8401 gataaaggaa ccgctgcgtt agcaggagta actggttact tagttatgac tgcaacgatc
     8461 aaagctttgg taaaactttt tatggcagaa ggatctgcaa ttgatactgg agttattgga
     8521 gcattagttg tcggaatagt tgccgtatat ttgcacaacc gatataacaa tattcaatta
     8581 ccttccgctt taggattctt tggaggttca cgcttcgttc ctattgttac atcgttctct
     8641 tctatcttga ttggctttgt cttctttgtt atttggccac ctttccaaca acttcttgtt
     8701 tctacaggtg gatatatttc tcaggcgggt ccaattggaa cttttctata tggattttta
     8761 atgagacttt ctggagcagt aggcttacat catataattt accctatgtt ttggtatact
     8821 gaacttggtg gtgttgaaac tgttgcagga caaacagtgg ttggagctca aaaaatattt
     8881 tttgctcaat tagccgattt ggcccattct ggattattta cagaaggaac aaggtttttt
     8941 gcaggtcgtt tctcaacaat gatgttcggt ttaccggctg cctgtttagc gatgtaccat
     9001 agtgttccta aaaatcgtcg taaaaaatac gcgggtttgt tttttggagt tgctttaaca
     9061 tcttttatta ccggtattac agaaccaatt gaatttatgt ttctattcgt cagtccggtt
     9121 ctatatgttg ttcacgcatt ccttgatggt gttagcttct ttattgcaga cgtcttaaat
     9181 atttcaatag gaaacacatt ttcaggaggt gtaatcgatt tcactttatt tggaattttg
     9241 caggggaacg ctaagacgaa ttgggttctt cagattccat ttggacttat ttggagtgtt
     9301 ttgtattata ttatttttag atggttcatt actcaattca acgttctaac gccagggcga
     9361 ggagaagaag tagattctaa agaaatttct gaatccgcag attcaacttc aaatactgca
     9421 gattatttaa aacaggatag cctacaaatt atcagagcct tgggtggatc aaataatata
     9481 gaagatgtag atgcttgtgt gacacgttta cgtgtagctg taaaagaagt taatcaagtt
     9541 gataaagcac ttttaaaaca aattggtgca gttgatgtct tagaagtgaa gggtggcatt
     9601 caagcaatct atggagcaaa agcaatctta tataaaaata gtattaatga aattttaggt
     9661 gtagatgatt aagtacttac tgacttaata aaaaacagag gagagtgatg gatgagtagg
     9721 atgaaatgaa atcgcataca agaaataaag aactcattat ccaagttgga tacgcttatt
     9781 acataggaga atacaaatga aatttagaaa attagcttgt acagtacttg cgggtgctgc
     9841 ggttcttggt cttgctgctt gtggcaattc tggcggaagt aaagatgctg ccaaatcagg
     9901 tggtgacggt gccaaaacag aaatcacttg gtgggcattc ccagtattta cccaagaaaa
     9961 aactggtgac ggtgttggaa cttatgaaaa atcaatcatc gaagcgtttg aaaaagcaaa
    10021 cccagatata aaagtgaaat tggaaaccat cgacttcaag tcaggtcctg aaaaaatcac
    10081 aacagccatc gaagcaggaa cagctccaga cgtactcttt gatgcaccag gacgtatcat
    10141 ccaatacggt aaaaacggta aattggctga gttgaatgac ctcttcacag atgaatttgt
    10201 taaagatgtc aacaatgaaa acatcgtaca agcaagtaaa gctggagaca aggcttatat
    10261 gtatccgatt agttctgccc cattctacat ggcaatgaac aagaaaatgt tagaagatgc
    10321 tggagtagca aaccttgtaa aagaaggttg gacaactgat gattttgaaa aagtattgaa
    10381 agcacttaaa gacaagggtt acacaccagg ttcattgttc agttctggtc aagggggaga
    10441 ccaaggaaca cgtgccttta tctctaacct ttatagcggt tctgtaacag atgaaaaagt
    10501 tagcaaatat acaactgatg atcctaaatt cgtcaaaggt cttgaaaaag caactagctg
    10561 gattaaagac aatttgatca ataatggttc acaatttgac ggtggggcag atatccaaaa
    10621 ctttgccaac ggtcaaacat cttacacaat cctttgggca ccagctcaaa atggtatcca
    10681 agctaaactt ttagaagcaa gtaaggtaga agtggtagaa gtaccattcc catcagacga
    10741 aggtaagcca gctcttgagt accttgtaaa cgggtttgca gtattcaaca ataaagacga
    10801 caagaaagtc gctgcatcta agaaattcat ccagtttatc gcagatgaca aggagtgggg
    10861 acctaaagac gtagttcgta caggtgcttt cccagtccgt acttcatttg gaaaacttta
    10921 tgaagacaaa cgcatggaaa caatcagcgg ctggactcaa tactactcac catactacaa
    10981 cactattgat ggatttgctg aaatgagaac actttggttc ccaatgttgc aatctgtatc
    11041 aaatggtgac gaaaaaccag cagatgcttt gaaagccttc actgaaaaag cgaacgaaac
    11101 aatcaaaaaa gctatgaaac aatagtcctt agttattcta taaaaagtag ttttttaaag
    11161 aacctaagag tgtatacccc cttttccctc tacacagata gtgtaagaaa agggggcttt
    11221 tgtttaaaat gtaagaaact gtcacgaaat taaaatgaag ttcttacata agcgaatcat
    11281 aaaaaatttc attttgattt taaaacagtt caagaaagtc aaaaaattat tctatttgaa
    11341 agagaggtgc cgactgtgaa agtcaataaa atccgtatgc gggaaacagt gatttcctac
    11401 gctttcctag caccagtatt attcttcttt gtcatctttg tgttggctcc gatggtgatg
    11461 ggcttcatta caagtttctt taactactca atgactaaat ttgagtttgt aggcttggat
    11521 aactatatcc gtatgtttaa agatcctgtc tttacaaaat ctctgattaa cacagttatt
    11581 ttggttattg gatctgtacc agttgttgtt ctattctcac tctttgtagc atctcagacc
    11641 tatcatcaaa atgtcattgc cagatccttc taccgtttcg tcttcttcct tcctgttgta
    11701 acgggtagtg ttgccgtgac agttgtttgg aaatggattt atgacccact atcagggatt
    11761 ctaaactttg tccttaagtc cagccacatc atcagccaaa acatttcttg gttgggagat
    11821 aaaaactggg cattgatggc gattatgatt attctcttga ccacttcagt tggtcagccc
    11881 atcatccttt atatcgctgc catggggaat attgacaatt cactggttga agcggcgcgt
    11941 gttgatggtg caactgagtt tcaagttttt tggaagatta aatggccaag ccttcttcca
    12001 acaactcttt atattgcaat catcacaaca attaactcat tccagtgttt cgccttgatt
    12061 cagcttttga catctggtgg tccaaactac tcaacaagta ccttgatgta ctacctttac
    12121 gaaaaagcct tccaattgac agaatacggc tatgccaaca caattggtgt cttcttggca
    12181 gtcatgattg ctatcgtaag ctttgttcaa tttaaagtac ttggaaacga cgtagaatac
    12241 taaagaaagg agacagctat gcaatctaca gaaaaaaaac cattaacagc ctttactgtt
    12301 atttcaacaa tcattttgct cttgttgact gtgctgttca tctttccatt ctactggatt
    12361 ttgacagggg cattcaaatc acaacctgat acaattgtta ttcctcctca gtggttccct
    12421 aaaatgccaa ccatggaaaa cttccaacaa ctcatggtgc agaaccctgc cttgcaatgg
    12481 atgtggaact cagtatttat ctcattggta accatgttct tagtttgtgc aacctcatct
    12541 ctagcaggtt atgtattggc taaaaaacgt ttctatggtc aacgcattct atttgctatc
    12601 tttatcgctg ctatggcgct tccaaaacaa gttgtccttg taccattggt acgtatcgtc
    12661 aacttcatgg gaatccatga tactctctgg gcagttatct tgcctttgat tggatggcca
    12721 ttcggtgtct tcctcatgaa acagttcagt gaaaatatcc ctacagagtt gcttgaatca
    12781 gctaaaatcg acggttgtgg tgagattcgt accttctgga gtgtagcctt cccgattgtg
    12841 aaaccagggt ttgcagccct tgcaatcttt accttcatca atacttggaa tgactacttc
    12901 atgcaattgg taatgttgac ttcacgtaac aatttgacca tctcacttgg ggttgcgacc
    12961 atgcaggctg aaatggcaac caactatggt ttgattatgg caggagctgc ccttgctgct
    13021 gttccaatcg tcacagtctt cctagtcttc caaaaatcct tcacacaggg tattactatg
    13081 ggagcggtca aaggataata ctctgcgaaa atctcttcaa actacgtcag cgtcaccttg
    13141 ccatacttaa gtattgcctg cggttagctt cctagtttgt tcttcaattt tcattgagta
    13201 taggaaaatc aatctatcaa gatacagaag tatattttat agatttagag aatatagagg
    13261 ttataagtgt ctacaaaatg gagggtatgc agttacttta tgaagttttg tcagacactt
    13321 ataaacttaa gaatggtttt agttaactat cagaaacgaa ggaaagagta tgatttttga
    13381 cgatttgaaa aacatcacct tttacaaagg gattcatcct aatttagaca aggctatcga
    13441 ctatctctac caacatcgta aggattcttt cgaattagga aagtatgaga ttgatggaga
    13501 taaagtcttt ctagttgttc aggaaaatgt cctcaatcaa gctgaaaatg atcaatttga
    13561 gtatcataag aactatgcag atttgcattt gctggtagaa ggacatgaat attcgagcta
    13621 cggttcacgt atcaaagacg aggcagtagc attcgacgaa gcgagtgaca ttggctttgt
    13681 tcattgtcat gaacactacc cactcttgtt gggttatcac aattttgcga ttttcttccc
    13741 aggtgagcca catcagccaa atggttatgc aggcatggaa gaaaaggttc gaaaatatct
    13801 ctttaaaatt ttgattgatt aaaaatagga tgaattgttt ttttgtaaag ctttgacaat
    13861 actctaccat gaaattgatc tttgtgaggt agagaaatga gaataaaata tttaaaaatt
    13921 ggtatcttct aagcatgctg caagagctag tttcttagat ggacagggaa ttacagttga
    13981 tgagacgtcc tggataatta atggcattgt gaatgcattg attggtagat acataaaatt
    14041 aggtacttat gcagctaagt atggtattag tatagcacgc tcgatcttaa gtagggcagc
    14101 tgcaactgca gcagcaagag taggattact gaccaagatt tctggatgga ttttacgagt
    14161 agctgtgaat gtagctgatg tatatggtaa ttttgccaac aatattgctg cagcttggga
    14221 tgcatatgat aaaattccta acaatggtcg tataaacttt taaaatgcga gaatgaaagc
    14281 actttgtatt tttttattga atatgttagc ttggacagtg cttgcaatga taattcgtgg
    14341 agggctagat ggatttgatg agcatacttg gagtactatt ttaattgctt cgctgttcgg
    14401 ggtatatgat tataagccca tagataaaaa tagaaaaaag tccaaaagaa aaaagaaaaa
    14461 atagatttgt tcatggtagg gacttatgaa agctttactg acaaaaaaga aaacagttta
    14521 caaagaaaaa tgatggagga gcaaacatgg cacaaaaagg agtaagcctt atcaaggcag
    14581 catttgatac agataacttt ctcatgcgtt ttagtgagaa agtcttggac atcgtgacag
    14641 ccaatcttct ttttgtcatc tcttgtttgc ccatcgtgac gattggagtg gctaaaatca
    14701 gcctctacga gaccatgttc gaagttaaga agagcagacg ggtgcctgtt tttaaaatct
    14761 atctaagatc tttcaagcaa aatctgaaac taggtcttca gctgggttta atggagttag
    14821 gaattgtgtt tcttaccctt tcagacctct atcttttctg gggtcaaaca gctctgccct
    14881 tccaattgct gaaagccatt tgtttaggta ttctgatttt tcttactatc gtgatgctgg
    14941 ctagttaccc tatcgcggca cgttatgacc tatcttggaa agaaattctt caaaaaggat
    15001 tgatgttggc tagttttaac tttccttggt tcttcctcat gttagccatt cttgtcctca
    15061 ttgtgatggt tctttatctg tccgccttca gtctactctt aggtggctca gtcttcctac
    15121 tttttgggtt tggactattg gtctttatcc agactggatt gatggagaaa attttcgcaa
    15181 aataccaata ggagccttat ttctgaaact actttcaaag gttccaaacg ctattctata
    15241 agcgagaaac taaaatctaa gtgtctataa gatattgaaa gccttttcgg taaagtgtat
    15301 actaaagaag taaagaaaaa tctgctttag cagaaaataa aaataatagg agaaaatcta
    15361 tgtcagattt gaaaaaatac gaaggtgtca ttccagcctt ctacgcatgt tatgatgatc
    15421 aaggagaagt aagcccagaa cgtacgcgtg ccttggttca atacttcatt gataaaggtg
    15481 ttcaaggtct ttatgtcaat ggttcttctg gtgaatgtat ctaccaaagc gttgaagatc
    15541 gcaagttgat tttggaagaa gttatggcgg ttgccaaagg taaattgacc attattgccc
    15601 atgttgcctg caacaatacc aaggatagta tggaacttgc tcgccatgct gaaagcttgg
    15661 gagtagatgc tattgcaacg attccaccaa tttatttccg cttgccagaa tactcagttg
    15721 ccaaatactg gaacgatatc agttctgcag ctccaaatac agactacgtg atttataaca
    15781 ttcctcaatt ggcaggtgtt gctttgactc caagccttta cactgaaatg ttgaaaaatc
    15841 ctcgtgttat cggtgtgaag aactcttcta tgccagttca agatattcaa acctttgtga
    15901 gccttggtgg ggaagaccat atcgtcttta acggtccaga tgaacagttc ctaggtggtc
    15961 gcctcatggg tgccaaagct ggtatcggtg gtacttatgg tgctatgcca gaactcttct
    16021 tgaaactcaa tcagttgatt acggataagg acctagaaac agcgcgtgaa ttgcagtatg
    16081 ctatcaacgc aatcattggt aaactcactt ctgctcatgg aaatatgtac ggtgtcatca
    16141 aagaagtctt gaaaatcaat gaaggcttga atattggatc tgttcgttca ccattgacac
    16201 cagtgactga agaagatcgt ccagttgtag aagcggctgc tgccttgatt cgtgaaacca
    16261 aggagcgctt cctctaatct aaaaggaggt atttatgaca tattacgttg caattgatat
    16321 cggtggaacc aacatcaagt atggtttggt tgatcaagag gggcaacttc ttgaatcgca
    16381 tgaaatgcca actgaggcgc ataagggtgg acctcatatc ttacaaaaga ccaaagatat
    16441 cgtagctagt tatttagaaa aaggcccagt agcaggtgtt gccatatctt ctgctgggat
    16501 ggtggatccg gataagggtg agattttcta tgctgggccg caaatcccta actacgcagg
    16561 cacccagttc aaaaaggaaa tcgaagaaag ctttactatt ccttgtgaga ttgaaaatga
    16621 tgtcaactgt gcaggtcttg ctgaggcagt atctggttca ggcaagggag caagtgtgac
    16681 actttgcttg accattggaa caggtatcgg tggttgcttg attatggata ggaaagtctt
    16741 ccatggtttt agcaattcag cctgtgaagt cgggtatatg catatgcagg atggagcttt
    16801 tcaagacttg gcttctacaa cagctttagt gaaatatgta gctgaagccc atggagaaga
    16861 tgttgatcag tggaatggcc gtagaatttt caaagaagcc actgaaggaa acaaaatctg
    16921 catggaaggt attgaccgta tggttgacta tctaggaaaa ggtctggcaa atatttgcta
    16981 cgttgccaat ccagaagtgg tcattcttgg tggtggtatc atggggcaag aggctatcct
    17041 caaacctaag atccgcacag ccttgaaaga ggctttggta ccaagtttag cagaaaaaac
    17101 acgattagaa tttgcccatc accaaaatac agcagggatg ttgggtgcat attatcattt
    17161 taagacaaaa caatcctagt ttggctcagc caaactagga ttttcttaca cgtttttgtc
    17221 tacgatagcc gttgagtttt ttattttccc agtagctatt aaagattttt tccttgcttt
    17281 cgcgattgat ttccaaaaag taggcataaa tcaaatcgat aaagaagagc ataggaagtt
    17341 gagcggatat tcgttggatg taggagggtt ggctgtgggt ggctacaaga acagtctctg
    17401 tataggtctg gctattttta ttgggaacac ttgtaaagag tacagtcttt gcccccatct
    17461 ccttggcatc taatagacta tctaaaatag aaggagttga gcctgaaagt gagaagccca
    17521 gtactagaca attttcatcc atgatgctgg ttgtccaggc aaagccgtct tggtctgtca
    17581 aagcttcgca gaccacacct aatcgcataa aacgtaattt catttcacgg gcgacgaggc
    17641 cagaactccc tgttccaaag aagtagatac gctcagcatc ttcgattagc tgggcaattc
    17701 gctctagttg gatttcgtca atcaagtctt gtgtttgttc cctcatattg ctataacttc
    17761 tgaggactcg tttggtcagt ggactgtgct tggagacttg gttggcttga ttttctgcct
    17821 gatgttggta ttggaaaata aattctcggt agccagtaaa gccacacttt ttagcaaagc
    17881 gggtcaaagc agcttgagaa atatgtaatt tttgggtgac ttgttgagaa gataaatcat
    17941 ctgtaatcgt ttcagcttgc aaaaaatagc gagcgatttc ttgctctagg tctgtcattt
    18001 cttcaaaatg tgaatcaatg atagttgcga tatctggttt gtccataggg aaagctcctt
    18061 tacatgagtc atactggaag actagatcag ggaatagtca cacttcatta taacacataa
    18121 tataaggata gataaataaa aacgcatctc tgttttaaaa acgaaaaaat cgaaaaagct
    18181 tctctctttt ccataatttt ctactcaaat tgtggtacaa ttaagagtaa gattttaagt
    18241 tagaaatgag actgatttgt atgagaaaat ttaacagcca ttcgattccg attcggctta
    18301 atttattgtt ttcaatcgtc attttactct ttatgaccat tattggtcgt ttgttgtata
    18361 tgcaggtttt gaacaaggat ttttacgaaa aaaagctagc ctcagctagt cagaccaaga
    18421 ttacaagcag ttcagcccgt ggggaaattt atgatgctag tggaaaacct ttggtagaaa
    18481 atacgttaaa gcaggttgtt tcctttacgc gtagcaataa aatgacggct acagacttaa
    18541 aagaaacagc taaaaagtta ctgacttatg tgagcatcag ttctccaaat ttgacagaac
    18601 gccagctggc ggattactat ttggctgatc ctgaaatcta taaaaaaata gtggaagctc
    18661 tcccaagtga gaaacgcttg gattcagatg gcaatcgtct atccgaatca gaactgtata
    18721 acaatgcggt cgatagtgta caaacgagtc aactaaacta tacagaggat gaaaagaaag
    18781 aaatctatct ttttagtcag ttaaatgctg ttggaaactt tgcgacagga accattgcga
    18841 cagatcctct aaatgattct caggtggctg ttattgcctc tatttcaaag gagatgcctg
    18901 gcattagtat ttctacttct tgggatagaa aggttttgga aacttccctt tcttctatag
    18961 ttgggagtgt atccagtgaa aaagctggtc tcccagcgga agaagcagaa gcctatctta
    19021 aaaaaggcta ttctctaaat gatcgtgtag gaacctccta tttggaaaag caatatgaag
    19081 agaccttaca aggaaaacgc tcggtaaaag aaatccatct ggataaatat ggcaatatgg
    19141 aaagcgtgga tacaattgag gaaggtagta agggaaacaa tatcaaactg accattgatt
    19201 tggccttcca agatagcgtg gatgctttac tgaaaagtta tttcaattcc gagctagaaa
    19261 atggtggagc caagtattct gaaggtgtct atgcagtcgc ccttaaccca aaaacaggtg
    19321 cggttttgtc tatgtcaggg attaaacatg acttgaaaac gggagagttg acgcctgatt
    19381 ccttgggaac ggtaaccaat gtctttgttc caggttcggt tgtcaaggcg gcgaccatca
    19441 gctcaggttg ggaaaatgga gtcttgtcag gaaaccagac cttgacagac cagtccattg
    19501 tcttccaagg ttcagctccc atcaattctt ggtatactca ggcttacggt tcattcccta
    19561 tcacagcggt ccaagctctg gagtattcat ctaataccta tatggtccaa acagccttag
    19621 gtcttatggg gcaaacctat caacccaata tgtttgtcgg caccagcaat ctagagtctg
    19681 ctatggagaa actgcgttca acctttggcg aatatggctt gggtactgcg acaggaattg
    19741 acctaccaga tgaatctact ggatttgttc ccaaagagta tagctttgct aattacatta
    19801 ctaatgcctt tgggcagttt gataactata cgccgatgca gttggctcag tatgtagcaa
    19861 ctattgcaaa taatggtgtt cgtgtggctc ctcgtattgt tgaaggcatt tatggtaata
    19921 atgataaggg aggactgggt gacttgattc agcaactgca accgacagag atgaataagg
    19981 tcaatatatc cgactccgat atgagcatct tgcaccaagg tttttatcag gttgcccatg
    20041 gtactagtgg attgacaact ggacgtgcct tttcaaatgg tgccttggta tccattagcg
    20101 gaaaaacagg tacagccgaa agctatgtgg cagatggtca gcaagcaacc aataccaatg
    20161 cggtggccta tgccccatct gataatcccc aaatcgctgt cgcagtggtc tttcctcata
    20221 ataccaatct aacaaatggt gtaggacctt ccattgcgcg tgacattatc aatctgtatc
    20281 aaaaatacca tccaatgaac tagaaaggaa attatgcttt atccaacacc tattgccaag
    20341 ttgattgaca gttattctaa gttaccaggt atcgggatta agacggctac gcgtctggcc
    20401 ttttatacga ttgggatgtc tgctgatgat gtcaatgaat ttgcaaaaaa tctcctttct
    20461 gctaagagag aattgacata ttgttctatt tgtggacgtt tgacagacga cgatccttgt
    20521 tctatctgta ccgatccgac tcgtgaccag acaacaattt tagttcttga ggatagtaga
    20581 gatgtggcaa ccatggaaaa tatccaagaa taccatggcc tctatcatgt cctgcatggc
    20641 ctcatttctc ctatgaatgg tatcagtccg gacgatatca atctcaagag ccttatgact
    20701 cgtcttatgg atagtgaagt ttcagaagtg attgtggcga ctaatgctac agcggatggt
    20761 gaagcgactt ccatgtatct ttcacgtttg ctcaagccgg ctggtatcaa ggttacgcgt
    20821 ctagcacgag gtctcgctgt gggagcggac attgagtatg cggacgaagt gacactctta
    20881 cgagccattg aaaatcggac agagttgtaa gtgtaggcaa atttacgaac tccattcatt
    20941 tataaaaaat caaagaggct gaaaatcgtt cctatcggcc tctttttgta tagtgtgatg
    21001 agtaggatcg ggttcaagtt ttaaaaaacc aagcaaatat gatatactaa agagcgagta
    21061 ttctagtaga attaggacaa ataatatgaa acaaacgatt attcttttat atggtggacg
    21121 gagtgcggaa cgcgaagtct ctgtcctttc agctgagagt gtcatgcgtg cggtcaatta
    21181 cgaccgtttc acagtcaaga ctttctttat cagtcagtca ggtgacttta tcaaaacaca
    21241 ggaatttagt catgctccgg ggcaagaaga ccgtctcatg accaatgaaa ccattgattg
    21301 ggataagaaa gttgcaccaa gtgctatcta cgaagaaggt gcagtggtct ttccagtcct
    21361 tcacgggcca atgggagaag atggctctgt tcaaggattc ttggaagttt tgaaaatgcc
    21421 ttacgttggt tgcaacattt tgtcatcaag tcttgccatg gataaaatca cgactaagcg
    21481 tgttctggaa tctgctggta ttgcccaagt tccttatgtg gctatcgttg aaggcgatga
    21541 tgtgactgct aaaatcgctg aagtggaaga aaaattggct tatccagtct tcactaagcc
    21601 gtcaaacatg gggtctagtg tcggtatttc taagtctgaa aaccaagaag aactccgtca
    21661 agccttaaaa cttgccttcc gatatgacag ccgtgtcttg gttgagcaag gagtgaatgc
    21721 ccgtgaaatt gaggttggcc tcttgggtaa ctacgatgtc aagagcacgc tacctggaga
    21781 agttgtcaag gacgttgcct tttatgacta cgatgccaag tatattgata acaagattac
    21841 tatggatatt cctgccaaaa tcagtgatga tgtggtagct gtcatgcgtc aaaatgcaga
    21901 aacagccttc cgtgccattg gtggccttgg tctatctcgt tgcgatttct tctatacaga
    21961 taagggagag atttttctca acgagctcaa taccatgcca ggtttcaccc agtggtctat
    22021 gtacccacta ctttgggaca atatggggat cagctaccca gaactaatcg agcgtttggt
    22081 tgaccttgcc aaggaaagtt ttgacaagcg cgaagcgcat ttgatataaa atgaaagaga
    22141 gggtagaagc cagaaccatc actgcaaggt gactagagtt ctcggacttc agcccttttt
    22201 aaaggagtag aaatgaaatt aacaatccat gaagttgccc aagttgttgg agccaaaaat
    22261 gatatcagta tctttgagga cacccagtta gaaaaagctg agtttgatag tcgtttgatt
    22321 ggaactggag atttatttgt gcccctaaaa ggtgtgcgtg acggtcatga ctttatcgaa
    22381 agagcttttg aaaatggtgc ggcagcaacc ctgtctgaga aagaggttgc aaatcatccc
    22441 tacattctag tagatgatgt tttgactgcc tttcaacaac tagctgccta ctatcttgaa
    22501 aaaacagctg ttgatgtctt tgcggttacg ggttcaaatg gcaagacaac gactaaggat
    22561 atgttggcgc atttactgtc aacaagatac aagacctaca aaacacaagg caattacaat
    22621 aatgagattg gccttcctta cacagttctc cacatgcctg aaggaacaga aaagttggtc
    22681 ttggagatgg ggcaggacca cttgggagat attcatctct tgtctgaatt ggctcgtcca
    22741 aaaacagcca tcgtgacctt ggttggagaa gctcatttgg cctttttcaa agatcgttct
    22801 gagattgcta aaggaaaaat gcaaattgca gacggaatgg cttcaggttc cttgctttta
    22861 gcgccggctg accctatcgt agaggactat ttgccaactg ataaaaaggt ggttcgtttt
    22921 ggtcaaggag cagagctgga aattactgac ttggttgagc gcaaagatag tctgaccttc
    22981 aaggccaatt tcttagagca agtccttgat ttgccagtaa ctggcaagta caatgcgaca
    23041 aatgctatga ttgcatccta tgttgccttg caagaaggag tttcagagga gcaaattcgt
    23101 ttggccttcc aacatcttga attgacgcgt aaccgtaccg agtggaagaa agcagccaat
    23161 ggagcagaca tcttgtcaga tgtttacaat gccaatccaa ctgctatgaa gctgatttta
    23221 gagaccttct ctgccattcc agccaatgaa ggtggcaaga aaattgcagt gttggcggat
    23281 atgaaggaac ttggtgtcca gtctgttcaa cttcataatc agatgatttt gagcctttcg
    23341 ccagatgtgc ttgatactgt tattttctac ggagaagata ttgctgaatt agcccaactt
    23401 gctagtcaaa tgttcccaat cggacacgtt tattacttca agaaaacaga agaccaagac
    23461 caatttgaag acctagttaa gcaggtcaaa gaaagccttg gggctcatga ccaaatcctg
    23521 ctcaaaggct ctaactctat gaatctagcc aagttggtag aaagtttaga aaatgaagac
    23581 aagtgatttt gtcaagtatt tgcaaagaat gattgccatt acagatactg gattaacctt
    23641 tacaaaagat ccgtttgacc gtgagcgcta cgaagacttg cgagaccttc tatctgaaat
    23701 gttgaatcaa gcatcagacc ttgattccga agaagtggca gaagtcttga agccaacttc
    23761 tgcttatgcg actcccttaa tggacgtccg tgcttggatt gttgaggacg aaaagatttg
    23821 tctggttagg ggacaaggag aggatagttg ggctttgcca ggtggctttg gtgaagtcgg
    23881 ctattctcca actgaaaata ttctcaagga aattgaagaa gaaaccggtt ttaaagccaa
    23941 agttgaaaga ctgctagctg tttttgatac caatcgtttc caactacaga gcaaacaata
    24001 tgcaaagttt gtttttgaat gtaagcttct cgatggacaa ttccaagaaa atcaagaaat
    24061 cgctgacctt caattttttg caattgaccc actgccgaat ttatctgaaa aacgcattac
    24121 caaggagcaa atcgagattc tttggcaggt ttatcaaggt cagagggagc aatatcttga
    24181 ctaagaagat gattatcgta tttctaaatt catttttctt aagtagcatg gtataataaa
    24241 atgcaggaaa attttgaatc atgaggaaga ctagatgaat ttatgggata ttttctttac
    24301 gactcaggca accgagccgc ccaaatttga ccttttttgg tatgttagcc tatttacgct
    24361 cttagcctta accttttata cagcccatcg ctatcgtgaa aagaaggttt accaacgatt
    24421 tttccaaatc ttgcagactg ttcagttaat ccttctttat ggttggtact gggtcaatca
    24481 tatgccactg tcagaaagcc taccctttta ccattgccgt atggctatgt ttgtggtact
    24541 cttgcttcct ggtcagtcca aatataaaca atactttgca ttattgggaa catttgggac
    24601 attagcagcc tttgtttatc cagtgccaga tgcttaccct tttccacata tcaccattct
    24661 atcctttatc tttggtcatt tagcactctt ggggaactct ctagtttatc tattgagaca
    24721 gtataatgcg cgattgctgg atgtgaaggg aatttttctc atgacctttg ccctaaatgc
    24781 cttgattttt gtggtcaatt tggtgacagg tggcgattac ggatttttga caaaaccgcc
    24841 attggttggg gatcacggtc tagtagctaa ttatttactt gtttcaattg tgctggtagc
    24901 tactatcagt ttgactaaga aaatcttaga attcttttta gctcaagaag cagaaaaaat
    24961 gattgcaaag gaagcttaac acagagcttt cttttttgct cttagagagt ttttacaagc
    25021 agcttataaa ataagaattt ctgaatagac aaactcaaaa aatggctggg aaatttagga
    25081 aaaaagcaag cacgattaaa ttttttgtgt tataatattt tgtgaatagc tatgcctatg
    25141 tttagctatg gaataatacg aagtgcgaaa cttggaagat agagaggaag cgatgtaatg
    25201 gctagagaag gcttttttac aggtctagat attggaacaa gctctgtcaa ggtgcttgtg
    25261 gccgagcaga gaaatggtga attaaatgta attggcgtga gtaatgccaa aagtaaaggt
    25321 gtaaaggatg gaattattgt tgatattgat gcagcagcaa ctgctatcaa gtcagccatt
    25381 tcccaagcgg aagaaaaggc aggcatttcg attaaatcag taaatgtcgg cttgcctggt
    25441 aatcttttgc aggtagaacc aactcagggg atgattccag taacatctga tactaaggaa
    25501 attacggatc aagatgttga aaatgttgtc aaatcagctt tgacaaagag tatgacacct
    25561 gaccgtgaag tcattacctt tattcctgaa gaatttattg tggatggttt ccaagggatt
    25621 cgtgacccac gtggcatgat gggggttcgc cttgaaatgc gtggtttgct ttatacagga
    25681 cctcgtacta tcttgcacaa tttgcgtaag acggttgagc gtgcaggtgt tcaggttgaa
    25741 aatattatca tttcaccact agcaatggtt cagtctgttt tgaatgaagg ggaacgtgaa
    25801 tttggtgcta cagtgattga tatgggggca ggtcaaacga ctgtcgctac aatccgtaat
    25861 caagaactcc agttcacaca tattctccaa gaaggtggag attatgtaac taaagatatc
    25921 tccaaggttt tgaaaacctc tcgcaaatta gcggaaggct tgaaactgaa ttacggggaa
    25981 gcctatccgc ctcttgcaag caaagaaacc ttccaagtag aggttattgg agaagtggaa
    26041 gcagtcgaag tgacggaagc ctacttgtca gaaattattt ctgcacgaat caagcacatc
    26101 cttgaacaaa tcaagcaaga attagataga aggcgtctat tggacctccc tggtggtatt
    26161 gtcttaatcg gtgggaatgc cattttacca ggtatggttg agcttgctca ggaagtcttt
    26221 ggcgtccgtg tcaagcttta tgttccaaat caagttggta tccgtaatcc agcctttgcg
    26281 catgtgatta gtttatcaga atttgcgggt caattaacag aagttaatct tttggctcag
    26341 ggagcgataa aaggtgagaa tgacttaagt catcagccaa ttagttttgg tgggatgctg
    26401 caaaaaacag ctcagtttgt acaatcaacg cctgttcaac cagctcctgc tccagaagta
    26461 gagtcggtgg cgcctacaga accaatggcg gatttccaac aagcttcaca aaataaaccg
    26521 aaattagcag atcgtttccg tggcttgatc ggaagcatgt ttgacgaata aagaggaaaa
    26581 ataaattatg acattttcat ttgatacagc tgctgctcaa ggggcagtga ttaaagtaat
    26641 tggtgtcggt ggaggtggtg gcaatgccat caaccgtatg gtcgacgaag gtgttacagg
    26701 cgtagaattt atcgcagcaa acacagatgt acaagcattg agtagtacaa aagctgagac
    26761 tgttattcag ttgggaccta aattgactcg tggtttgggt gcaggaggtc aacctgaggt
    26821 tggtcgtaaa gccgctgaag aaagcgaaga aacactgacg gaagctatta gtggtgccga
    26881 tatggtcttc atcactgctg gtatgggagg aggctctgga actggagctg ctcctgttat
    26941 tgctcgtatc gccaaagatt taggtgcgct tacagttggt gttgtaacac gtccctttgg
    27001 ttttgaagga agtaagcgtg gacaatttgc tgtagaagga atcaatcaac ttcgtgagca
    27061 tgtagacact ctattgatta tctcaaacaa caatttgctt gaaattgttg ataagaaaac
    27121 accgcttttg gaggctctta gcgaagcgga taacgttctt cgtcaaggtg ttcaagggat
    27181 taccgatttg attaccaatc caggattgat taaccttgac tttgccgatg tgaaaacggt
    27241 aatggcaaac aaagggaatg ctcttatggg tattggtatc ggtagtggag aagaacgtgt
    27301 ggtagaagcg gcacgtaagg caatctattc accacttctt gaaacaacta ttgacggtgc
    27361 tgaggatgtt atcgtcaacg ttactggtgg tcttgactta accttgattg aggcagaaga
    27421 ggcttcacaa attgtgaacc aggcagcagg tcaaggagtg aacatctggc tcggtacttc
    27481 aattgatgaa agtatgcgtg atgaaattcg tgtaacagtt gttgcaacgg gtgttcgtca
    27541 agaccgcgta gaaaaggttg tggctccaca agctagatct gctactaact accgtgagac
    27601 agtgaaacca gctcattcac atggctttga tcgtcatttt gatatggcag aaacagttga
    27661 attgccaaaa caaaatccac gtcgtttgga accaactcag gcatctgctt ttggtgattg
    27721 ggatcttcgc cgtgaatcga ttgttcgtac aacagattca gtcgtttctc cagtcgagcg
    27781 ctttgaagcc ccaatttcac aagatgaaga tgaattggat acacctccat ttttcaaaaa
    27841 tcgttaagta aatgaatgta aaagaaaata cagaacttgt ttttcgagaa gttgcagagg
    27901 ctagtctgag tgctcatcga gagagtggtt cggtctctgt cattgcagtt accaagtatg
    27961 tagatgtacc gacagcggaa gccttgcttc cgctaggtgt tcatcatatc ggtgaaaatc
    28021 gtgtagataa gtttctggaa aaatatgaag ctttaaaaga tcgagatgtg acttggcatt
    28081 tgattggtac cttgcaaaga cgtaaggtga aagatgtcat tcaatacgtt gattatttcc
    28141 atgcattgga ctcagtaaag ctagcagggg aaattcaaaa aagaagtgac cgagtcatca
    28201 agtgtttcct tcaagtaaat atttctaaag aagaaagcaa acacggtttt tcgagagagg
    28261 aactgctgga aatcttgcca gagttagcca gactagataa gattgaatat gttggtttaa
    28321 tgacgatggc accttttgag gctagcagtg agcagttgaa agagattttc aaggcgaccc
    28381 aagatttaca aagagaaatt caagagaaac aaattccaaa tatgcctatg accgagttaa
    28441 gtatgggaat gagtcgtgat tataaagaag cgattcaatt cggttccact tttgttcgta
    28501 taggtacatc attttttaag taggagagaa ccatgtcttt aaaagataga ttcgatagat
    28561 ttatagatta ttttacggag gatgaggatt caagtctccc ttatgaaaaa agagatgagc
    28621 ctgtgtttac tccagtaaat tcttcacagg aaccggctct cccaatgaat caaccttcac
    28681 agtcggctgg tacaaaagag aacaatatca ccagacttca tgcaagacaa caggaattgg
    28741 caaatcagag tcagcgtgca acggataagg tcattataga tgttcgttat cctagaaaat
    28801 atgaggatgc aacagaaatt gttgatttat tggcaggaaa cgaaagtatc ttgattgatt
    28861 ttcagtatat gacagaggtg caggctcgtc gttgtttgga ctatttggat ggagcttgtc
    28921 atgttttagc tggaaatttg aaaaaggtag cttctaccat gtatttgttg acaccagtga
    28981 atgttattgt aaatgttgaa gatatccgtt taccagatga agatcaacag ggtgagttcg
    29041 gttttgatat gaagcgaaat agagtacgat aatgattttt ttaattcgta tgatttataa
    29101 tgcagtggat atttactccc tgattttggt agccttcgct gtcatgtctt ggtttccagg
    29161 tgcctacgaa tccagtttag gtcgttggat tgtagcgttg gtgaaaccag tgcttgctcc
    29221 cttgcaacgc ctgcctttac agatagcggg tcttgattta tctgtttggg tcgcgattgt
    29281 tttggttcga tttttaggag aaaacctagt gcgttttctg gcgatgatag gatgaataaa
    29341 gggatttatc agcatttctc catagaagat cgtccatttc ttgacaaggg aatggaatgg
    29401 ataaagaagg tagaagatag ctatgctcct tttttaactc cttttatcaa tcctcatcag
    29461 gagaagctat taaagatttt ggccaaaacc tatggtcttg cttgtagcag tagtggggaa
    29521 ttcgtctcga gtgagtatgt tcgagtttta ttatacccag attatttcca accagagttt
    29581 tcagattttg aaatatctct ccaggaaatt gtgtattcca ataaatttga acatttaacg
    29641 catgctaaga ttttagggac agtcatcaat caattaggga ttgaacggaa actttttgga
    29701 gatatcctag tagatgaaga acgggcgcag attatgatta atcagcagtt tcttcttctc
    29761 tttcaagatg gactaaagaa aattggtcgt atacctgttt cgctggagga acgtcctttc
    29821 accgagaaaa tagataagct agaacagtat cgagaactgg atttatctgt gtctagtttt
    29881 cgattagatg ttcttttatc aaatgttttg aaactatcta ggaatcaagc aaaccagttg
    29941 attgaaaaga aacttgtcca agtaaattat catgtggtag acaaatcaga ttacactgtt
    30001 caagttggag acttgattag tgtgagaaaa tttggtcgct tgagattact tcaagataag
    30061 ggacaaacga aaaaagagaa gaaaaaaata accgtccagt tattattaag taagtgagga
    30121 atagaatgcc aattacatca ttagaaataa aggacaagac ttttggaact cgattcagag
    30181 gttttgatcc agaagaagtc gatgaatttt tagatattgt ggttcgtgat tacgaagatc
    30241 ttgtgcgtgc gaatcatgat aaaaatttgc gtattaagag tttagaagag cgtttgtctt
    30301 actttgatga aataaaagat tcattgagcc agtctgtatt gattgctcag gatacagctg
    30361 agagagtgaa acaggcggcg catgaacgtt caaacaatat cattcatcaa gcagagcaag
    30421 atgcgcaacg cttgttggaa gaagctaaat ataaggcaaa cgagattctt cgtcaagcaa
    30481 ctgataatgc taagaaagtc gctgttgaaa cagaagaatt gaagaacaag agccgtgtct
    30541 tccaccaacg tctcaaatct acaattgaga gtcagttggc tattgttgaa tcttcagatt
    30601 gggaagatat tctccgtcca acagctactt atcttcaaac cagtgatgaa gcctttaaag
    30661 aagtggttag cgaagtactt ggagaaccga ttccagctcc aattgaagaa gaaccaattg
    30721 atatgacacg tcagttctct caagcagaaa tggaagaatt acaagctcgt attgaggtag
    30781 ccgataaaga attgtctgaa tttgaagctc agattaaaca ggaagtggaa actccaactc
    30841 ctgtagtgag tcctcaagtt gaagaagagc ctctgctcat ccagttggcc caatgtatga
    30901 agaaccagaa gtagctccag tgcatccgac aggtccaaca ccagctacag aaactgttga
    30961 ttcagcaccg ggatttgaag caccgcaaga atctgttaca attttataag aaatattctg
    31021 agaacaatat cttatcctta tattcccagc gagcaggaga tggtgtgagt cctgcattcc
    31081 ctatcgataa gattatcctc tcaaactatc aagtctgaat ctagtaagat ttgacgttcc
    31141 ccacgttacg ggataagagg gagaaagact aaaacttttt ccgaataaag gtggtaccac
    31201 gattttcgtc ctttttggaa gtcgtggttt ttaatttgtt attatttata aaggagatac
    31261 catgaaactc aaagacaccc ttaatcttgg gaaaactgaa ttcccaatgc gtgcaggcct
    31321 tcctaccaaa gagccagttt ggcaaaagga atgggaagat gcaaaacttt atcaacgtcg
    31381 tcaagaattg aaccaaggaa aacctcattt caccttgcat gatggccctc catacgctaa
    31441 cggaaatatc cacgttggac atgctatgaa caagatttca aaagatatca ttgttcgttc
    31501 taagtctatg tcaggatttt acgcaccatt tattcctggt tgggatactc atggtctgcc
    31561 aatcgagcaa gtcttgtcaa aacaaggtgt caaacgtaaa gaaatggact tggttgagta
    31621 cttgaaactt tgccgtgagt acgctctttc tcaagtagat aaacaacgtg aagattttaa
    31681 acgtttgggt gtttctggtg actgggaaaa tccatatgtg accttgactc ctgactatga
    31741 agcagctcaa attcgtgtat ttggtgagat ggctaataag ggttatatct accgtggtgc
    31801 taagccagtt tactggtcat ggtcatctga gtcagccctt gctgaagcag agattgaata
    31861 ccatgacttg gtttcaactt ccctttacta tgccaacaag gtaaaagatg gcaaaggagt
    31921 tctagataca gatacttata tcgttgtctg gacaacgact ccatttacta tcacagcctc
    31981 tcgtggtttg acggttggtg cagatattga ttacgttttg gttcaacctg ctggtgaagc
    32041 tcgtaagttt gtcgttgctg ctgaattatt gactagcttg tctgagaaat ttggctgggc
    32101 tgatgttcaa gttttggaaa cttaccgtgg ccaagaactc aaccacatcg taacagaaca
    32161 cccatgggat acagctgtag aagagttggt aattcttggt gaccacgtta cgactgactc
    32221 tggtacaggt attgtccata cagcccctgg ttttggtgag gacgattaca atgttggtat
    32281 tgctaataat cttgaagtcg cagtgactgt tgatgaacgt ggtatcatga tgaagaatgc
    32341 tggtcctgaa tttgaaggtc aattctatga aaaggtagtt ccaactgtta ttgaaaaact
    32401 tggtaacctc cttcttgccc aagaagaaat ctctcactca tatccatttg actggcgtac
    32461 taagaaacca atcatctggc gtgcagttcc acaatggttt gcctcagttt ctaaattccg
    32521 tcaagaaatc ttggacgaaa ttgaaaaagt gaaattccac tcagaatggg gtaaagtccg
    32581 tctttacaat atgatccgtg accgtggtga ctgggttatc tctcgtcaac gtgcttgggg
    32641 tgttccactt cctatcttct acgctgaaga tggtacagct atcatggtag ctgaaactat
    32701 tgaacacgta gctcaacttt ttgaagaaca tggttcaagc atttggtggg aacgtgatgc
    32761 caaagacctc ttgccagaag gatttactca tccaggttca ccaaacggcg agttcaaaaa
    32821 agaaactgat atcatggacg tttggtttga ctcaggttca tcatggaatg gagtggtggt
    32881 aaaccgtcct gaattgactt acccagccga cctttaccta gaaggttctg accaataccg
    32941 tggttggttt aactcatcac ttatcacatc tgttgccaac catggcgtag caccttacaa
    33001 acaaatcttg tcacaaggtt ttgcccttga tggtaaaggt gagaagatgt ctaaatctct
    33061 tggaaatacc attgctccaa gcgatgttga aaaacaattc ggtgctgaaa tcttgcgtct
    33121 ctgggtaaca agtgttgact caagcaatga cgtgcgtatc tctatggata tcttgagcca
    33181 agtttctgaa acttaccgta agattcgtaa cactcttcgt ttcttgattg ccaatacatc
    33241 tgactttaac ccagctcaag atacagtcgc ttacgatgag cttcgttcag ttgataagta
    33301 catgacgatt cgctttaacc agcttgtcaa gaccattcgt gatgcttacg caaactttga
    33361 attcttgaca atctacaagg ccttggtgaa ctttatcaac gttgacttgt cagccttcta
    33421 ccttgatttt gccaaagatg ttgtttacat tgaaggtgcc aaatcactgg aacgccgtca
    33481 aatgcagact gtcttctatg acattcttgt caaaatcacc aaactcttga ctccaatcct
    33541 tcctcacact gcggaagaaa tctggtcata tcttgagttt gaagccgaag acttcgtcca
    33601 attgtcagaa ttaccagaag ttcaaacttt tgctaaccaa gaagaaatct tggatacatg
    33661 ggcagccttc atggacttcc gtggacaagc acaaaaagcc ttggaagaag ctcgtaatgc
    33721 aaaagttatc ggtaaatcac ttgaagcaca cttgacagtt tatccaaatg aagttgtgaa
    33781 aactctactc gaagcagtaa acagcaatgt agctcaactt ttgatcgtgt ctgagttgac
    33841 catcgcagaa gaaccagctc cggaagctgc ccttagcttc gaagatgtag tcttcacagt
    33901 tgaacgcgct gcaggtgaag tatgtgaccg ttgccgtcgt attgacccaa caacagcaga
    33961 acgtagctac cacgcagtca tctgtgacca ctgtgcaagt atcgtagaag aaaactttgc
    34021 agatgcagtc gcagaaggat ttgaagagaa ataagattga aaagtctagg taaaactcaa
    34081 tttgagaaga aaagacaact aattttatag tctattaaac gcattgtatc acgtttttga
    34141 acacctgata tgatgcgttt tttatttatt ttaaaaattt gcgaggtatg actttttata
    34201 ctcaacaaga atcaaagaga aacttagcaa gctaacagta gtaatataaa ataggaattt
    34261 gatattaggg ataagattgg taaatagtgt aatattttta caacaataaa tttatatagt
    34321 tatttctggt ttctgaaaag tattatatta tattttattt catattatac aaatttttat
    34381 tttataatat cagaaaatac tttttttaaa aagcaaatat gatacaattt tatttgaaaa
    34441 aaaataaaaa aggagatttt attatgaaat caagtggtat cgtcggtctt gtgggaggaa
    34501 ttttacttct tatttgtcct tttgtcttgt tgggaacagc ggtaaataca gctgctacaa
    34561 ctcttaatgg aggagctact gcaggggctt tttcaggtgt tgccttactc ttgaatgcct
    34621 tgaagattgc aaatcttgtt cttggtatca ttgctattgt ttactataaa ggagataagc
    34681 atgtaggtgc agctccgtct gtactaatga ttgtttctgg tgaagttagt ctcattccat
    34741 tcttaggatg ggttgggggg attcttgcta ttatcggagg atctctattc cttgcaacat
    34801 tgaagaaatt caaatcagaa gaataaaagg tattttagca tgaaaagaac aaaaaagttt
    34861 atcggtatag gagtagctct attatctctt tctcttctag ttgcatgtgg aacataaagt
    34921 tcaaagaata cttcaacaag taatgatgag aagacagtag caacatccaa tagttcaaaa
    34981 gaaacaatca ctttcgatac accggttgta acagacgatg cgattgaatc aatacgcact
    35041 tatgcagatt atatagatct ttataaaaat atttttgatg attattttac taaagctgag
    35101 gaaggtttca aaggcacaac tatggaaaat aatgactcgt ttactaaact aaaagagtca
    35161 actcaaaaat tattcgatgc gcagaaaaaa aggttaaata atgaagatag aatagaaaca
    35221 accaaaaaca atgtgattgc caaacattgt caaacagtcc tttccttttt ggttttgact
    35281 agcttttttg tgaaaaattg tgtaaaatag aatagataaa cgaggggaaa cctcggaaaa
    35341 tttaaaggag aatccatcta atggtaaaat tggtttttgc tcgccacggt gagtctgaat
    35401 ggaacaaagc taaccttttc actggttggg ctgatgttga tttgtctgaa aaaggtacac
    35461 aacaagcgat tgacgctggt aaattgatca aagaagctgg tatcgaattt gaccaagctt
    35521 acacttcagt attgaaacgt gctatcaaaa caactaactt ggctcttgaa gcttctgacc
    35581 aattgtgggt tccagttgaa aaatcatggc gcttgaacga acgtcactac ggtggtttga
    35641 ctggtaaaaa caaagctgaa gctgctgaac aatttggtga tgagcaagtt cacatctggc
    35701 gtcgttcata cgatgtattg cctccaaata tggaccgtga tgatgagcac tcagctcaca
    35761 cagaccgtcg ttatgcttca cttgacgact cagtcattcc agatgctgaa aacttgaaag
    35821 tgactttgga acgtgctctt ccattctggg aagataaaat cgctccagct cttaaagatg
    35881 gtaaaaacgt attcgtagga gctcacggta actcaatccg tgcccttgta aaacacatca
    35941 aaggtttgtc agatgatgag atcatggacg tggaaatccc taacttccca ccattggtat
    36001 tcgaattcga cgaaaaattg aacgttgttt ctgaatacta ccttggaaaa taaaaaattg
    36061 taagcctagg attgatttct aggcttttta tgttagtatg gaagtatgat aaggaataaa
    36121 aaacaagatt atgtactggc ctacaagcaa ccagcttcaa ccacttacat gggttgggaa
    36181 gaagaagctt taccgatagg caatggttct ttaggagcaa aagtatttgg ccttataggg
    36241 gctgaacgga ttcaatttaa tgaaaaaagt ctctggtctg gaggtccact tcctgatagt
    36301 tcagattatc agggtggaaa tcttcaggat cagtatgttt ttttagctga gattcggcag
    36361 gctttggaga agagagatta caatctggct aaggaactgg ctgagcagca cctaattggg
    36421 ccaaaaacga gtcaatatgg gacctatctg tcttttgggg atattcacat tgagttcagc
    36481 cagcaaggta cgactttgtc tcaggtgacg gactatcaga gacagctgaa tattagtaag
    36541 gcacttgcga cgacttctta tgtctataag ggaacgcgat ttgaacgtaa agcttttgcg
    36601 agttttccag atgatctctt gattcaatgt tttactaagg aagggttgga aactctagat
    36661 tttactatag aactatcctt gacctgtgat ttggcttctg atggaaagta tgagcaggaa
    36721 aaatctgatt acaaggagtg taagttggat attactgatt ctcatatctt gatgaaggga
    36781 agagttaagg ataatgatct gcggtttgct agttatctag cttgggaaac ggatggagat
    36841 attagagttt ggtcagatag ggttcagata tcaggagcca gttatgccaa tctcttcttg
    36901 gccgctaaga cggattttgc ccaaaatcct gctagcaatt atcgcaagaa actagattta
    36961 gagcaacagg tgatagactt ggtggacaca gctaaagaaa agggctatac ccaattgaaa
    37021 tcaaggcata tcgaggacta ccaagcctta ttccagcgtg ttcaattgga tttggaagct
    37081 gatgttgacg catccactac agatgatttg ttaaaaaatt ataagccaca agaagggcag
    37141 gctttggagg agctgttctt ccagtatgga cggtatttat tgattagttc gtccagagac
    37201 tgcccagatg ctctaccagc taacctacag ggagtctgga atgcggtcga caatcctcct
    37261 tggaattcgg actatcactt aaatgtcaat ctgcagatga attattggcc agcctatgtt
    37321 accaatctcc tagagacggt ctttccagtc atcaactatg tagatgattt gcgtgtctat
    37381 ggtcgtctag cggctgtaaa gtatgcagga atcgtctctc aaaaaggtga ggaaaatggt
    37441 tggttggttc atactcaagc gactcccttt ggttggacgg cacctggttg ggattactat
    37501 tggggttggt caccagctgc caatgcgtgg atgatgcaaa ccgtttatga agcctattca
    37561 ttttataggg accaagacta tctcagggag aaaatttatc ccatgttgag ggaaacggtt
    37621 cgtttttgga atgccttttt acataaggat cagcaggcgc agcgttgggt ctcttctccg
    37681 tcttattccc cagaacatgg gccgatttcg attggcaata cctatgacca atctctgatt
    37741 tggcagttat ttcatgattt tattcaggct gctcaggaat tgggactgga tgaggacttg
    37801 ttgactgagg ttaaggagaa gtctgattta ctaaatcctt tgcaaatcac tcaatctggt
    37861 cgaatcaggg agtggtatga ggaggaagag cagtattttc aaaatgagaa agtggaggcc
    37921 cagcatcggc acgcttccca tctagtggga ctctattctg gcaatctctt tagctacaag
    37981 ggacaagagt atattgaagc ggcgcgtgct agcctcaatg atcgtggaga tggcggcaca
    38041 ggctggtcca aggctaataa gatcaatctc tgggcgcgtt tgggagatgg caatcgagcc
    38101 cataaattat tggcagagca gttaaagaca tccaccttgc caaatctttg gtgtagccat
    38161 cctccttttc agatagatgg taattttggt gctactagtg gcatggcaga aatgttactc
    38221 cagtctcatg cagcttatct ggtacctcta gctgccctac ctgatgcttg gtcaacaggt
    38281 tctgtttcag gcttaatggc acgtggacat tttgaagtga gcatgagctg ggaagataaa
    38341 aaactcttac agttgaccat tttatcaagg agtggaggag atttgcgagt ttcttatcca
    38401 gatattgaga agagtgtgat taaaatgaat caagaaaaaa taaaagcgaa atgcatgggg
    38461 aaagattgta tttcggtggc aacagcagaa ggtgatcttg ttcaatttta tttttaagaa
    38521 gatgttataa ggcagtaatt tgaaacttcc ttttaataag gatttgagaa tataggcagt
    38581 ttccaactag ttgaaaaagc gttataatga taataggaag taatactcaa tgaaaatcaa
    38641 agagc
//
LOCUS       DAVBCW010000008        34992 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVBCW010000008 DAVBCW010000000
VERSION     DAVBCW010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34992
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.008"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            49..303
                     /locus_tag="VQ514_000352"
     CDS             49..303
                     /locus_tag="VQ514_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET2499170.1"
                     /translation="MHRTTIPYFVSQEVYWKVRNIGAEAIRRNCERGAIFSGKIKHHE
                     DSQFKGDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            647..1981
                     /gene="trmFO"
                     /locus_tag="VQ514_000353"
     CDS             647..1981
                     /gene="trmFO"
                     /locus_tag="VQ514_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HET2499171.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVANHPLIEVVRDEITELPTDVITVIATGPLTSDALAEKIHALNDGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRIFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEAIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            2065..2802
                     /locus_tag="VQ514_000354"
     CDS             2065..2802
                     /locus_tag="VQ514_000354"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HET2499172.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            2811..3368
                     /gene="frr"
                     /locus_tag="VQ514_000355"
     CDS             2811..3368
                     /gene="frr"
                     /locus_tag="VQ514_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HET2499173.1"
                     /translation="MANAIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRIHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVV
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            3428..4282
                     /gene="cvfB"
                     /locus_tag="VQ514_000356"
     CDS             3428..4282
                     /gene="cvfB"
                     /locus_tag="VQ514_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HET2499174.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTSLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            4291..4506
                     /locus_tag="VQ514_000357"
     CDS             4291..4506
                     /locus_tag="VQ514_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HET2499175.1"
                     /translation="MRKSFYTWLMTERNPKSNNPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            4592..5560
                     /locus_tag="VQ514_000358"
     CDS             4592..5560
                     /locus_tag="VQ514_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET2499176.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMALVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            5740..6240
                     /locus_tag="VQ514_000359"
     CDS             5740..6240
                     /locus_tag="VQ514_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2499177.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVMDFVYAYPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIA
                     EQSLED"
     gene            6243..6503
                     /locus_tag="VQ514_000360"
                     /pseudo
     CDS             6243..6503
                     /locus_tag="VQ514_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(6870..7981)
                     /gene="ald"
                     /locus_tag="VQ514_000361"
                     /pseudo
     CDS             complement(6870..7981)
                     /gene="ald"
                     /locus_tag="VQ514_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253293.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
     gene            8158..8727
                     /locus_tag="VQ514_000362"
     CDS             8158..8727
                     /locus_tag="VQ514_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2499178.1"
                     /translation="MESIFVKFAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKDNGKFIGTIDLHKIDPVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKANPASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            8795..9445
                     /locus_tag="VQ514_000363"
     CDS             8795..9445
                     /locus_tag="VQ514_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET2499179.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTSVKETNLQ
                     AEVAAVSKDSSTGKEVNKEEKEEPVEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSTSKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGAKTIEKLKDYVTVD"
     gene            9429..11669
                     /locus_tag="VQ514_000364"
     CDS             9429..11669
                     /locus_tag="VQ514_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HET2499180.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSVSYFALLGFVFLLVCL
                     FIQFPWKSAGKVLVICGVFGFWFLFQTWQQSQVSQNLVDSVERVRILPDTIKVNGDSL
                     SFRGKSDGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFDYQGY
                     LKTQGIYQTLNIKRIQSLQKVGSWDIGENLSSLRRKAVVWIKMHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMDGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     VFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVANRPLVFGQPNEWLLILLLI
                     SLALVYDLRKNIKKLTVLCLLITGLFLLTKHPLENEITMMDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIKKWQEKMTTSNAQRSLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     SEMTKAFHVGEILVSKDSLKQKEFVAELQATQTKVRSMIVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            11849..12037
                     /locus_tag="VQ514_000365"
     CDS             11849..12037
                     /locus_tag="VQ514_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499181.1"
                     /translation="MTVTKSYKYDWNTVLEYSTNYHDHQYAWIPEWSRYYSYSEYKVG
                     GGWNYARYEVINYYTGGY"
     gene            12069..12656
                     /locus_tag="VQ514_000366"
     CDS             12069..12656
                     /locus_tag="VQ514_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2499182.1"
                     /translation="MLNLTHVTLKTRQVILQDADFTFKKGRIYGLLAINGSGKTTLFR
                     AMSKLLPLSSGHIAVPPSLFYYESVEWLDGNLSGMDYLRLIKNIWKSDLNLRDEIAYW
                     EMADYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDICDRVVTIHQGQIEEV"
     gene            12660..13835
                     /locus_tag="VQ514_000367"
     CDS             12660..13835
                     /locus_tag="VQ514_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499183.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIILALFVSVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWKDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLPKQIDNVDLNWSMGMVLLPCLIIFLLLGILFIERWGSSQKKRIF"
     misc_feature    14009..14140
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            14156..14686
                     /gene="infC"
                     /locus_tag="VQ514_000368"
     CDS             14156..14686
                     /gene="infC"
                     /locus_tag="VQ514_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HET2499184.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            14719..14919
                     /gene="rpmI"
                     /locus_tag="VQ514_000369"
     CDS             14719..14919
                     /gene="rpmI"
                     /locus_tag="VQ514_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HET2499185.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            14971..15330
                     /gene="rplT"
                     /locus_tag="VQ514_000370"
     CDS             14971..15330
                     /gene="rplT"
                     /locus_tag="VQ514_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HET2499186.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            15388..15768
                     /locus_tag="VQ514_000371"
     CDS             15388..15768
                     /locus_tag="VQ514_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET2499187.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            16018..16818
                     /locus_tag="VQ514_000372"
     CDS             16018..16818
                     /locus_tag="VQ514_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HET2499188.1"
                     /translation="MNPTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVNQRVCEDGPVFRTGTVVL"
     gene            16781..17767
                     /locus_tag="VQ514_000373"
     CDS             16781..17767
                     /locus_tag="VQ514_000373"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HET2499189.1"
                     /translation="MVLFSAQEQLYYKEKIMTTNRLQVSLPGLDLKNPIIPASGCFGF
                     GQEYAKYYDLNLLGSIMIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAE
                     KLPWLEREYPNLPIIANVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGL
                     LIGQDPDLAYDVVKAAVEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVG
                     MRFDLKTRKPILANGTGGMSGPAVFPVALKLIRQVAQTTNLPIIGMGGVDSAEAALEM
                     YLAGASAIGVGTANFTNPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            17809..19578
                     /locus_tag="VQ514_000374"
     CDS             17809..19578
                     /locus_tag="VQ514_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HET2499190.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKIAEKEWVYDS
                     HSQAWYYFKSGGYMAKNETVDGYQLGSDGKWLGGKATNENAAYYQVVPVTANVYDSDG
                     EKLSYISQGSVVWLDKDRKSDDKRLAITISGLSGYMKTEDLQALDASKDFIPYYESDG
                     HRFYHYVAQNASIPVASHLSDMEVGKKYYSADGLHFDGFKLENPFLFKDLTEATNYSA
                     EELDKVFSLLNINNSLLENKGATFKEAEEHYHINALYLLAHSALESNWGRSKIAKDKN
                     NFFGITAYDTTPYLSAKTFDDVDKGILGATKWIKENYIDRGRTFLGNKASGMNVEYAS
                     DPYWGEKIASVMMKINEKLGGKD"
     gene            complement(19639..21294)
                     /gene="pavA"
                     /locus_tag="VQ514_000375"
     CDS             complement(19639..21294)
                     /gene="pavA"
                     /locus_tag="VQ514_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HET2499191.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEI
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTKSLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPLANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            21399..21896
                     /gene="ybeY"
                     /locus_tag="VQ514_000376"
     CDS             21399..21896
                     /gene="ybeY"
                     /locus_tag="VQ514_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HET2499192.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLGYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            21877..22272
                     /locus_tag="VQ514_000377"
     CDS             21877..22272
                     /locus_tag="VQ514_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HET2499193.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFGFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            22289..23188
                     /gene="era"
                     /locus_tag="VQ514_000378"
     CDS             22289..23188
                     /gene="era"
                     /locus_tag="VQ514_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HET2499194.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPDRFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            23237..24061
                     /gene="mutM"
                     /locus_tag="VQ514_000379"
     CDS             23237..24061
                     /gene="mutM"
                     /locus_tag="VQ514_000379"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HET2499195.1"
                     /translation="MPELPEVETVCRCLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARSSQTLTAEEATAIHDQTIAVLSQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQGCVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            24061..24666
                     /locus_tag="VQ514_000380"
     CDS             24061..24666
                     /locus_tag="VQ514_000380"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HET2499196.1"
                     /translation="MGKIIGITGGIASGKSTVTNFLRQQGFQVVDADAVVHQLQKPGG
                     RLFEALVQHFWQEIILENGELNRPLLASLIFSNPDEREWSKQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            24653..25852
                     /locus_tag="VQ514_000381"
     CDS             24653..25852
                     /locus_tag="VQ514_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HET2499197.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSEQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            25852..26001
                     /gene="rpmG"
                     /locus_tag="VQ514_000382"
     CDS             25852..26001
                     /gene="rpmG"
                     /locus_tag="VQ514_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET2499198.1"
                     /translation="MRVKINLKCSSCGSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            26041..26274
                     /gene="secG"
                     /locus_tag="VQ514_000383"
     CDS             26041..26274
                     /gene="secG"
                     /locus_tag="VQ514_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HET2499199.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            26376..28730
                     /gene="rnr"
                     /locus_tag="VQ514_000384"
     CDS             26376..28730
                     /gene="rnr"
                     /locus_tag="VQ514_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HET2499200.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVSSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSHSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            28693..29160
                     /gene="smpB"
                     /locus_tag="VQ514_000385"
     CDS             28693..29160
                     /gene="smpB"
                     /locus_tag="VQ514_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HET2499201.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NIKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            29176..30036
                     /gene="tehB"
                     /locus_tag="VQ514_000386"
     CDS             29176..30036
                     /gene="tehB"
                     /locus_tag="VQ514_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HET2499202.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEDVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            30109..31062
                     /gene="coiA"
                     /locus_tag="VQ514_000387"
     CDS             30109..31062
                     /gene="coiA"
                     /locus_tag="VQ514_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HET2499203.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKKQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            31081..32883
                     /gene="pepF"
                     /locus_tag="VQ514_000388"
     CDS             31081..32883
                     /gene="pepF"
                     /locus_tag="VQ514_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HET2499204.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEVLKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQMEKLYVYAHMKNDQDTREAKYQEYYAKAMTFYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGIYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            32885..33598
                     /locus_tag="VQ514_000389"
     CDS             32885..33598
                     /locus_tag="VQ514_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HET2499205.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELENFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            33665..34606
                     /gene="prsA"
                     /locus_tag="VQ514_000390"
     CDS             33665..34606
                     /gene="prsA"
                     /locus_tag="VQ514_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HET2499206.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(34723..>34992)
                     /locus_tag="VQ514_000391"
     CDS             complement(34723..>34992)
                     /locus_tag="VQ514_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2499207.1"
                     /translation="QHDSYHRFLESKGIQASMSRKGNSQDNGRMESFFGILKSEMFYG
                     YEKTFKSLNQLEQAIIDYIDYYNNKRIKVKLKGLSPVQYRTKSFR"
BASE COUNT        10539 a         6494 c         7895 g        10064 t
ORIGIN      
        1 aacaagacgc ttaaatcgtg catcggttag ttgtttactt gcttcataat tcatagaact
       61 actataccat attttgtttc gcaggaagtc tattggaaag taagaaatat tggagctgag
      121 gcgattagaa gaaattgtga gcgtggtgct attttttcag gtaaaataaa acatcacgaa
      181 gattctcagt ttaaaggaga ccactatgtt gaatgttatg ctgttttaga taatacggtt
      241 atagcaagag atagaataac agtccctatc gatccgttat gtggaaaaga ttttatagag
      301 tagcatataa ttgattctta actggaatac tctctatctc tttacatcaa gaaaatgact
      361 aaacagggaa gtttgccttc ttcccttttt ttgttatact agtagaagaa aaaaatagaa
      421 agatttgtgg gagtgaaaaa gtccggggga ctttttcagc ttgagccaag aaattcgaga
      481 gcgaggaaag tccgggggac tttttcagcc tgagccaaga aattcgagag cgaagtaagt
      541 ccaatggact ttttcagcct gagccaagaa actcgaaagc tcgtaagtct gattggcttt
      601 ttcaatgtga atcttaactt cacactccca aagaggtatt agtgtcgtgt ctcaatctta
      661 tatcaatgtt atcggtgctg gtttggcagg ttctgaagca gcttaccaaa tcgcagagcg
      721 tggtattcca gttaaactat atgaaatgcg tggtgtcaag tcgactcccc agcataaaac
      781 agacaatttt gctgagttgg tttgttccaa ttctttgcgt ggggatgctt tgacaaatgc
      841 agttggtctt ctcaaggaag aaatgcgtcg cttgggttct gttatcttgg aatctgctga
      901 ggctacacgt gttcctgcag gtggtgccct tgcagtggac cgtgatggtt tctctcaaat
      961 ggtgaccgaa aaagttgcca accacccctt gattgaagtg gttcgtgatg aaattacaga
     1021 attgccgaca gatgttatta cggttatcgc tactggtcct ttgacaagtg atgccttggc
     1081 tgaaaagatt catgccctta atgacggtga tggtttttat ttctacgatg cggcagcgcc
     1141 tattatcgat gtcaacacta tcgatatgag caaggtctac ctcaaatcac gttatgataa
     1201 gggagaagcg gcctacctca atgcccctat gaccaagcaa gaatttatgg atttccatga
     1261 agctttggtc aatgcagaag aagcaccgct taattctttt gaaaaagaaa agtactttga
     1321 aggctgtatg cctatcgaag tcatggccaa acgtggcatt aaaactatgc tttatggccc
     1381 tatgaagcca gtcggtcttg agtacccaga cgactataca ggacctcgtg atggagaatt
     1441 taaaacacct tatgcggttg tgcaacttcg tcaggacaat gcagctggta gcctctacaa
     1501 tattgttggt ttccagaccc acctcaaatg gggagaacaa aagcgtatct tccaaatgat
     1561 tccgggtctt gaaaatgcag agtttgtccg ttatggtgtc atgcatcgca attcttacat
     1621 ggattcacca aatcttcttg agcagactta ccgttctaag aaacaaccaa atctcttctt
     1681 tgctggtcag atgacggggg tggaaggcta tgttgagtcg gcggcttcag gcttagttgc
     1741 gggaattaac gcagctcgtc tcttcaagga agaaagcgag gctattttcc ccgagacgac
     1801 agcgattgga agcttagctc attacattac ccatgccgac agcaaacatt tccaaccaat
     1861 gaatgtcaat tttgggatca tcaaggagtt ggaaggcgag cgtatccgtg ataagaaggc
     1921 tcgttatgaa aaaattgcag agcgtgccct tgccgactta gaggaatttt tgactgtcta
     1981 atttttttga aagaattgct catgatacta taaaaatctt agaaattgtg ataaaatagg
     2041 taggatgaaa gaaggagagt gaaaatggcg aatcccaagt ataaacgtat tttaatcaag
     2101 ttatcaggtg aagcccttgc cggtgaacgt ggcgtaggga ttgatatcca aacagttcaa
     2161 acaatcgcaa aagagattca agaagttcat agcttaggta tcgaaattgc ccttgttatt
     2221 ggtggaggaa atctctggcg tggagaacct gcagcagaag caggtatgga ccgtgttcag
     2281 gcagattaca ctggaatgct tgggactgtt atgaatgctc ttgtgatggc agattcattg
     2341 caacaagttg gggttgatac gcgtgtacaa acagctattg ctatgcaaca agtggcagag
     2401 ccttatgtcc gtggacgtgc ccttcgtcac cttgaaaaag gccgtatcgt tatctttggt
     2461 gctggaattg gttcaccata cttctcgaca gatacaacag cggcccttcg tgcagctgaa
     2521 atcgaagcgg atgccatcct catggctaaa aatggtgtcg atggtgttta caatgccgat
     2581 cctaagaaag ataagacagc tgttaagttt gaagaattga cccaccgtga cgttatcaat
     2641 aaaggtcttc gtatcatgga ctcaacagct tcaaccctct caatggacaa cgacattgac
     2701 ttggttgtct tcaacatgaa ccaatcaggc aacatcaaac gtgtcgtatt tggtgaaaat
     2761 atcggaacaa cagtttcaaa taatatcgaa gaaaaggaat aagaaagaat atggctaacg
     2821 caattattga aaaagctaaa gagagaatga cccagtctca ccaatcactt gctcgtgaat
     2881 ttggtggtat ccgtgctggt cgtgccaatg caagcttgct tgaccgtata catgtagaat
     2941 actatggagt tgaaactcct cttaaccaaa tcgcttcaat tacgattcca gaagcgcgtg
     3001 ttttgttggt aacaccattt gacaagtctt cattgaaaga catcgaacgt gccttgaacg
     3061 cttctgatct tggtatcaca ccagctaatg acggttctgt gattcgcttg gttgtcccag
     3121 ctcttacaga agagactcgt cgtgaccttg ctaaagaagt gaagaaggtc ggcgaaaatg
     3181 ctaaagtggc tgtccgcaat atccgtcgcg atgctatgga cgaagctaag aaacaagaaa
     3241 aagcaaaaga aatcactgaa gacgaattga agactcttga aaaagacatt caaaaagtaa
     3301 cagacgatgc tgttaaacac atcgacgata tgactgccaa caaagagaaa gaacttttgg
     3361 aagtctaaaa ataaacagaa aaactcagtt ggcattgctg gctgagtttt attcgaaaga
     3421 aggaaatatg aatacaaatc ttgcaagttt tatcgttgga ctgatcatcg atgaaaacga
     3481 ccgtttttac tttgtgcaaa aggatggtca aacctatgct cttgctaagg aagaaggcca
     3541 acatacagta ggggatacgg tcaaaggttt tgcatacacg gatatgaagc aaaaactccg
     3601 cctgacaagc ttagaagtga ctgccactca ggaccaattt ggttggggac gtgtcacaga
     3661 ggttcgtaag gacttgggtg tctttgtgga tacaggcctt cctgacaagg aaatcgttgt
     3721 gtcactcgat attctccctg agctcaagga actctggcct aagaagggcg accaactcta
     3781 catccgtctt gaagtggata agaaagaccg tatctggggt ctcttggctt atcaagaaga
     3841 cttccaacgt cttgctcgtc ctgcctacaa caacatgcag aaccaaaact ggccagccat
     3901 tgtttaccgt ctcaagttat caggaacttt tgtttaccta ccagaaaata atatgcttgg
     3961 ttttattcat cctagcgagc gttacgcaga gccacgtttg gggcaagtat tagatgcgcg
     4021 cgttattggt ttccgtgaag tggaccgcac tttgaacctc tccctcaaac cacgctcctt
     4081 tgaaatgttg gaaaacgatg ctcagatgat tttgacttat ttggaaagca atggcggttt
     4141 catgacctta aatgacaagt catctccaga cgacatcaag gcaacctttg gcatttctaa
     4201 aggtcagttc aagaaagctt taggtggtct tatgaaggct ggtaaaatca agcaggacca
     4261 gtttggtaca gagttgattt agggaggctt atgagaaaat cattttacac ttggctcatg
     4321 accgagcgca atcctaaaag taacaatccc aaagcaattt tggcagacct cgcttttgaa
     4381 gaggcagctt ttccaaaaca tacagatgat tttgatgagg tcagtcgctt tttggaggag
     4441 catgccagtt tctcttttaa cctaggagat tttgacagca tttggcagga atatctagaa
     4501 cactagcatt tattcattgg gtttgggcta gtaatttctc catccctctg ctataataaa
     4561 aagaaataaa aggattagag aggttcttta tttgaaggaa cattcaatag acattcaact
     4621 gagtcatcca gatgacctgt ttcatctttt tggttccaat gaacgccatc ttcgtttgat
     4681 ggaagaagag cttgatgttg tgattcatgc tcgtacggag attgtccagg ttttgggaga
     4741 agagtctgct tgcgaggaag cccgtcaggt tattcaagct ctcatggcct tggtaaatcg
     4801 tgggatgacc gttggtacgc cagatgtagt gacggctatt agtatggtca aaaatgatga
     4861 aattgacaag tttgtcgccc tttacgaaga agaaatcatc aaggataata ctgggaagcc
     4921 tatccgcgtc aaaaccttag gtcaaaaact ttatgtggac agtgtcaaac agcatgatgt
     4981 gacctttgga attgggccag caggtacagg gaagaccttc cttgcagtga ccttggcagt
     5041 gactgccctt aaacgtgggc aagtcaagcg aattatccta actcgtccag cggtggaagc
     5101 gggagagagt cttggatttc ttccgggtga tcttaaggag aaggtggatc cttaccttcg
     5161 tcctgtttac gatgccttgt atcaaattct tgggaaagac caaacgactc gtctcatgga
     5221 gcgtgaaatt atcgaaattg cgccccttgc ctatatgcgt ggccggacct tggatgatgc
     5281 ctttgtcatt ctcgatgagg cgcaaaacac gaccatcatg cagatgaaga tgttcttgac
     5341 gcgtttaggc tttcattcta agatgattgt caatggagat atcagtcaga ttgacctgcc
     5401 acgtaatgtc aagtccggtt tgattgatgc tcaagagaaa ctcaagaaca tccatcagat
     5461 tgactttgtt catttttcag ccaaggatgt ggttcgccat ccagttgtcg ctcagattat
     5521 ccgagcctat gaatattcta ctgaagttgc acacgactga ttttgaggaa gttcgcctgc
     5581 aaaagaatat acttttgaga gaggaaaatc cagttgtata ggctaaaggt ttgagacaac
     5641 gattaggcac gatggaaaga acttttatgt ggctgatgac gatcagtgca tcttcctgtg
     5701 tcataatcac agggcacaag aaagtaggaa tttgaaaaga tgattgacca actatctaag
     5761 tattacagtt gtaggatact aactgaaaag gatattccaa gtattttatc tttatatgaa
     5821 agtaatcctc tgtattttca gcattgtcca ccagagccaa attttgcaac tgtaaaagag
     5881 gacatgcttt gtctacctga aggtaaagct aaggctgata agttttttgt tggattttgg
     5941 aatggctctg accttgtggc tgttatggat tttgtctatg catatcctga tgaggagact
     6001 gtttttattg gtttgtttat ggttgatcaa gcctatcaga ggaaagggat tggtagtcat
     6061 attgtgacag aagcactagc ttattttgct aagaactttc gaaaggcacg tttggcttat
     6121 gttaagggaa atccgcaatc tcagcatttt tgggaaaagc agggctttaa atcaattgga
     6181 tgcgaggtta agcaagaact ctatacggtt gttatcgctg aacagagcct agaagattag
     6241 aaatggcatc aagtaagaac tatttggaat ttgttttgga acaattatca ggattagatg
     6301 atgtgactta ccgttccatg attggggagt atattcttta cttccgcggc aagattattg
     6361 gcggcattta tgacgatcgc tttttagtta aacccgtgca agcagtctta gataagattg
     6421 accaatcttc ttttgagttt ccatacaaag gtgccaaaga aatgatttga gtggaagaac
     6481 ttgataataa gatgtttcta taagacctga ttttagctat gtataaccaa ctgccaacgc
     6541 ccaaacctaa aaagaaaaag caagggtgaa cgaagtaaaa aagaagtctg ctaaggccct
     6601 gtctttgcac gggtaaaatt ttatatataa aaagaagctg ggactaaaga gctcagcttc
     6661 ctttggttta tataattgtc attacaagac gaagtggttg ggcgaaactc tgttgacttt
     6721 attcaattta gagtttctta tgcacaattg agtctggaac gaaagtctcc agttgcaaag
     6781 tatacagtac aataaaccaa cgatgtaata gctgatgaca caaagcacag tgggtaggac
     6841 ttgcgaagtc acccttttct tttcaaaatt tatactaaat cattgatatc agtgtagtca
     6901 cgattaagtc cttgagcaac tggtaggcta gtcaagtaac cttgataagt ggtcacacct
     6961 tgacgcaagc cttcatcttc agagattgct tgtgcgaatc ctttgccagc caaagcttcg
     7021 atataaggaa gagtgacatt ggttagggcg atggttgaag tgcgggcaac cgcaccaggg
     7081 atattggcaa cggcatagtg gagaacaccg tgtttttcat agacgggttc atcgtgcgtt
     7141 gtcacacggt cagctgtttc gataacgcca ccttggtcaa cagcaacgtc aacgatacag
     7201 agcctggacg catttgtttg accatctcat ctgtcaccaa ttccggtgct tttgcaccag
     7261 ggatgagaat ggctccaatc accacatcag catctctcac acttgcttca atgttgaatg
     7321 aattagatat aatagtttga atttgacttc caaagacttc ttctagaact gagagacgct
     7381 tggcactaat atctaaaata gtcacttgag caccaagacc aagggcgatg cgggcagcat
     7441 gtgtaccgac gacaccatca ccgatgatag ttacttttcc ttttggaaca cctggtacac
     7501 caccaagtag aacaccagag ccaccagctt gcttagtaag gaagtgagct ccgatttgaa
     7561 cagccatacg acctgcaacc tcactcatag gaacgaggag cggtagttgt ccttgattgt
     7621 cacgaacagt ttcataggca attcctgttg tttttgctgc taacatagca tctgctaatt
     7681 ctggagcagc ggccatgtgc aagtaggtga agagaagaag atcgtcgcgc aagtaaccgt
     7741 attcagaact taaaggttct tttactttca caaccaactc tgctgcccaa gcttcaccag
     7801 cagtagcgac aatctcagct ccttgctttt gatagtcagc atcagtaaag ccagaaccga
     7861 gaccagcatt tgtttcgata aggacacgat gaccacgact aactaagcta tgaacacctg
     7921 caggtgtgag ggcgacacgg ttttcgttat ttttaatttc ttttgggatt ccgattaaca
     7981 ttgagataac ctacctttca attgacggtc ttgttttggt tgtcacattc cagttcataa
     8041 atcaaaaatg tgacggtttc attgtatatg aaaccgcttc aaaaatcaag aaaaacttgt
     8101 catccaaatt tttttatgct agactagtga aaatcaagct ctaatggagg gaaaagtatg
     8161 gaatcaatat ttgtgaaatt tgcccagtat ccgtctatag aaacggagcg tttattgctt
     8221 agacctgtaa ctttggatga tgcggaagca atgtttgact atgcctcgga caagggtaat
     8281 acacgttaca cttttccaac caatcaaagc ttggaagaaa ccaagaataa cattgctcag
     8341 ttctacttgg ctaatccctt gggacgttgg gggattgaat taaaagataa cggcaagttt
     8401 atcggaacta ttgacttaca caagattgat cctgttctta agaaggcagc tattggctac
     8461 attatcaata aaaagtattg gaatcaagga ttaacgacag aagccaatcg tgctgtgatt
     8521 gagctagctt ttgagaagat agggatgaat aagttgactg cccttcacga taaggctaat
     8581 cccgcgtcag gaaaggtcat ggagaaatca ggcatgcgtt tttcccatgc agaaccatat
     8641 gcttgtatgg accagcatga aaaaggccga atcgtgacaa gagttcatta tgtcttgacc
     8701 aaggaagact attttgcaaa taaataagca gttgaaaaga aatttttcga ctgttttttc
     8761 ttcctcttac gaataatcta agagaggaga aaatatggaa gcaattatcg agaaaatcaa
     8821 agagtataaa atcatcgtca tctgtactgg tctgggcttg cttgtaggag gatttttcct
     8881 gctaaagcca gctccacaaa catctgtcaa agagacgaat ttgcaggctg aagttgcagc
     8941 tgtttccaag gactcatcga ccggaaagga agtgaacaag gaagagaagg aagaaccagt
     9001 tgaacaagat ctaatcacag tagatgtcaa aggtgctgtc aaatcgccag ggatttatga
     9061 cttgcctgta ggtagtcgag tcaatgatgc tgttcagaag gctggtggct tgacagagca
     9121 agcagacagc aagtcgctca atctagctca gaaagttagt gatgaggctc tggtttacgt
     9181 tcctactaag ggagaagaag cagttagtca acagactggt tcggggacag cttcttcaac
     9241 aagcaaggaa aagaaggtca atctcaacaa ggccagtctg gaagaactca agcaggtcaa
     9301 gggactggga ggaaaacgag ctcaggacat tattgaccat cgtgaggcaa atggcaagtt
     9361 caagtcagta gacgagctca agaaggtctc tggcattggt gccaagacca tagaaaaact
     9421 taaagactat gttacagtgg attaagaatt tctctattcc cctaatttac ctgagttttc
     9481 tattactatg gctttactac gccattttct cagtatccta ttttgctttg ttgggttttg
     9541 tttttctgct agtctgcctc tttatccaat ttccttggaa atcagcaggt aaagttctag
     9601 tgatttgtgg agtctttggc ttctggtttc tgtttcaaac ttggcaacag agtcaagtga
     9661 gtcaaaattt ggtggattct gttgaaaggg tacggatttt accagacact attaaggtta
     9721 acggtgacag tctgtccttt cgtggtaagt ctgatggtcg tgctttccaa gtctattata
     9781 aactccagtc cgaggaggag aaagaagcct ttcaagcttt aactgacctt catgagatag
     9841 gactagaagg gaagctttcg gagccagaag ggcagagaaa ttttggtggc tttgactacc
     9901 aaggctatct gaagactcag ggaatttacc agacactcaa tattaaaaga atccagtcac
     9961 tccaaaaggt tggcagttgg gatataggtg aaaacctgtc cagtttacgt cgaaaggctg
    10021 tggtttggat taagatgcac ttcccagacc ctatgcgcaa ttacatgaca ggactcttgc
    10081 taggacatct ggacaccgat tttgaggaga tgaatgagct ttattccagt ttaggaatta
    10141 ttcacctttt tgccttgtca ggtatgcagg tagggttttt catggacgga tttaagaaac
    10201 tacttttacg attgggcttg acacaagaaa agttgaagtg gctgacttat cccttttccc
    10261 ttatctatgc gggactaact ggattttcag catcggttat tcgcagtctc ttgcaaaagc
    10321 tactggctca acatggggtt aagggcttgg ataattttgc cttgacggtg cttgtcctct
    10381 ttattgtcat gccaaacttt ttcttgacag caggaggagt cttgtcctgt gcttatgctt
    10441 ttatcctgac catgaccagc aaagaagggg aggggctcaa ggctgttact agtgaaagtc
    10501 tagtcatctc cttgggcata ttgcccattc tatccttcta ttttgcggaa tttcaacctt
    10561 ggtctatcct tttgaccttt gtcttttcct ttctttttga cttggtcttc ttaccgctct
    10621 tgtctatctt atttgtcctt tcctttctct atccagtcat tcagctgaac tttatctttg
    10681 aatggttaga gggcattatt cgcttggtct cgcaggtggc aaatagaccg cttgtctttg
    10741 gacaacccaa tgaatggctt ttaatcctat tgttaatttc tttggctttg gtctatgatt
    10801 tgaggaaaaa cattaaaaag ctaacggtat tgtgcttatt gattacaggg ctctttctcc
    10861 tgaccaagca tccactggaa aatgaaatca ccatgatgga tgtggggcaa ggagaaagta
    10921 ttttcctacg ggatgtaact gggaaaacca ttctcataga tgtaggtggt aaggcagaat
    10981 cttataagaa aatcaaaaaa tggcaagaaa agatgacgac cagcaatgcc cagcgaagct
    11041 tgatacccta tctcaaaagt cgaggagtag ctaagattga ccagctaatt ttgactaaca
    11101 cggacaagga gcatgttgga gatttgtcag agatgaccaa ggctttccat gtaggggaga
    11161 ttctagtatc aaaagacagt ctgaaacaga aggaatttgt ggcagaacta caggcgactc
    11221 aaacaaaggt gcgtagtatg atagtagggg agaacttacc catttttgga agtcagttag
    11281 aagttctatc tccaaggaaa atgggagatg gaggacacga tgatacccta gttctgtatg
    11341 ggaaattctt ggataagcaa tttctcttca cgggaaattt ggaggagaaa ggagagaagg
    11401 acttgctgaa gcactatcca gacttgaaag taaatgtttt gaaagctagc caacatggca
    11461 ataaaaaatc atcaagtcca gcctttctag aaaaactcaa accagagctt actcttatct
    11521 cagttggaaa gagcaatcga atgaaactcc cccatcagga aacattgaca cgactggaag
    11581 gtatcaatag caaagtttat cgaactgacc agcaaggagc tatacgtttt aaggggttgg
    11641 atagttggaa aatcgaaagt gttcgatagg aaggataaat gttgtagatt agtgaaataa
    11701 actaaaaatt tgttgcataa taatgataaa aacggcataa tgaaaacgta ttcaatattg
    11761 aggatataaa atcattaaaa atcagcaaaa gttgttttag ttagttagtt tataatctat
    11821 tggtcttctt cagtccagtg tatctgctgt gacagtcact aaaagttaca agtatgattg
    11881 gaatacggtt ttggaatata gtaccaacta tcacgaccat cagtatgctt ggattccgga
    11941 atggtctcgt tattacagct attctgagta taaagttggc ggaggctgga actacgctcg
    12001 ttatgaggtc ataaactact atactggagg ttattaatcc ttaaagagtg agaaaaggag
    12061 ggctggatat gttaaatctt actcatgtta ccttaaaaac gcgacaagtc atcttgcaag
    12121 atgcggattt tacctttaaa aagggtagga tttatggcct tcttgctatc aatggctcgg
    12181 gaaagacgac actattccga gctatgagca agttgcttcc ccttagtagt ggacacatcg
    12241 cagttcctcc ttctttgttt tattatgaga gcgttgaatg gctggatgga aacttaagtg
    12301 ggatggacta ccttcgtctc ataaaaaaca tctggaagtc agacctaaac ttgagagatg
    12361 aaatcgccta ctgggaaatg gctgactata tcagtcttcc catccgcaag tattccttag
    12421 ggatgaagca acgcttggtg attgctatgt attttctcag tcaggccaaa tgctggctca
    12481 tggatgagat tacaaatggc ttagacgagt attatcgaca gaagtttttt gataggctag
    12541 cacaaatcga tagacaagaa cagctggttc ttttaagttc ccactataag gaagagttgg
    12601 ttgatatctg cgatagagta gtaaccattc atcaggggca gatagaagag gtttagttta
    12661 tgaaagatgt tagtctattt ttattgaaaa aagttttcaa aagtcgttta aactggatta
    12721 tcttagcttt atttgtatct gtactcggtg ttacctttta tttaaatagt cagactgcaa
    12781 actcacacag cttggagagc aggttggaaa gtcgcattgc agccaacgag agggctatca
    12841 atgaaaatga agagaaactc tcccaaatgt ctgataccag ctcggaggaa taccagtttg
    12901 ctaaaaataa tttagacgtg caaaaaaatc ttttgacgcg aaagacagaa attctaactt
    12961 tactaaaaga agggcgctgg aaagaagcct actatctgca gtggaaagat gaagagaaga
    13021 attatgaatt tgtatcaaat gacccgactg ctagctctgg cttaaaaatg ggggttgacc
    13081 gcgaacggaa gatttaccaa gccctgtatc ccttgaacat aaaagcacat actttggagt
    13141 ttccgaccca cgggattgat cagattgtct ggattttaga ggttatcatc ccaagcttgt
    13201 ttgtggttgc gattattttt atgctaacac aactatttgc agaaagatat caaaatcatc
    13261 tggacacagc tcacttatat cctgtttcaa aagtgacatt tgcaatatcc tctcttggag
    13321 ttggagtggg atatgtaact gtgctgttta tcggaatctg tggcttttct tttctagtgg
    13381 gaagtctgat aagtggtttt ggacagttag attatcccta cccaatttat agcttagtga
    13441 atcaagaagt aactattggg aaaatacaag atgtattatt tcctggcttg ctcttagctt
    13501 tcttagcctt tatcgtcatt gtggaagttg tgtacttgat tgcttacttt ttcaagcaaa
    13561 aaatgcctgt cctctttctt tcactcattg ggattgttgg cttattgttt ggcatccaaa
    13621 caattcagcc tcttcaaagg attgcacatc tgattccctt tacttacttg cgttcagtgg
    13681 agattttatc tggaagatta cctaagcaga ttgataatgt cgatctaaat tggagcatgg
    13741 gaatggtctt acttccttgc ctgattatct ttttgctatt gggaattcta tttattgaaa
    13801 gatggggaag ttcacagaaa aaaagaattt tttaatagat tctagctttc ctataggtag
    13861 ggaaaataag taaaaactaa catagagagg gaatcaactt gattctctct ttttgattcg
    13921 aaaaccaaac caaaatacaa acacaaactt ttcaaaaaat aactttttat cttgacaaga
    13981 gctagaaaac ttggtatcat ataaaagttg agaaaagcag aagtgagagc ttctcgcctt
    14041 gtgacattaa gttgcctggc cctacggatg aaaagtttcg aagaaacgct atcataacgt
    14101 gcgggcttgt atatttacga gtccgctatt gtttttctct aataaaacaa aagaggtgaa
    14161 aaccatagca aagcaagact tattcatcaa tgatgagatt cgtgtacgtg aagttcgctt
    14221 gattggtctt gaaggagaac agctaggcat caagccactc agtgaagcgc aagctttggc
    14281 tgataacgct aatgttgacc tagtattgat tcaaccccaa gccaaaccgc ctgttgcaaa
    14341 aattatggac tacggtaagt tcaaatttga gtaccagaag aagcaaaaag aacaacgtaa
    14401 aaaacaaagc gttgttactg tgaaagaagt tcgtctaagt ccaactattg acaagggtga
    14461 ctttgataca aaacttcgta atgcacgtaa gttccttgaa aaaggaaata aagttaaggt
    14521 atctattcgc tttaagggtc gtatgattac ccataaagag attggtgcaa aagttttagc
    14581 cgagtttgct gaagcaactc aagatattgc catcatcgaa caacgtgcta aaatggatgg
    14641 acgtcaaatg ttcatgcaat tggcgccagc gactgacaaa aaataattgt cagaaagtta
    14701 aataaaggag aaaaaattat gccaaaacaa aaaacacacc gcgcatcagc taaacgtttc
    14761 aaacgtacag gttctggtgg acttaaacgt ttccgtgctt acacttctca ccgtttccac
    14821 ggaaaaacta agaaacaacg tcgtcatctt cgtaaagcat ctatggtgca ttcaggagat
    14881 tacaaacgta tcaaagcaat gcttactcgc ttgaaataat agcttgactg taagtaaaca
    14941 attctaggaa atatttggag gaaataaaac atggcacgtg ttaaaggtgg cgttgtatca
    15001 cgcaaacgtc gtaaacgtat tcttaaatta gcaaaaggtt actatggagc taaacacatc
    15061 ttgttccgta ctgcaaaaga acaagtaatg aactcttact actatgcata ccgtgaccgt
    15121 cgtcagaaaa aacgtgactt ccgcaaattg tggatcactc gtatcaatgc ggcagctcgt
    15181 atgaacggac tttcatactc acaattgatg catggtttga aattggctga gatcgaagtt
    15241 aaccgtaaaa tgcttgctga cttggctgtt aacgatgcag tagctttcac agctcttgca
    15301 gatgcagcta aagcaaaact tggtaaataa caactgatga gactggaaca ggattgtcca
    15361 gtctttttaa aaataaagga gaaaaatatg gcttcaaaaa tgctacacac ttgcttacga
    15421 gtagaaaatc ttgaaaaatc aatcgcattt tatcaagatg cttttggttt taaagaattg
    15481 cgtcgcagag attttccaga ccatgccttc acgattgtct atctaggtct tgagggtgat
    15541 gactatgagt tggagttgac ttataactac gatcacggtc cgtatgtggt tggtgatggg
    15601 tttgcccata tcgccctcag tacacctgat cttgaggcac ttcatcaaga acacagtgca
    15661 aaaggctatg aagtgactga gccaaatggt ctaccaggaa ctacaccaaa ctattacttt
    15721 gtcaaagacc ctgatggata taaggtcgaa gtcattcgtg aaaaataatt tcgtgcaagt
    15781 taagtagaat attcgttttt tacttgactt tttttagctg aaagagtata ctaataaaaa
    15841 ttcaaccttt aaggggagtc cagagagact cacaaggtgt cagataaaag aatagtgcaa
    15901 ttttctagag gagacttttt gagtgtgctc tcttgtgttg tacgatttta actgaggcct
    15961 tgcactagca aggtcttttc tttatctggt ccccttaaaa tttaaggagg aaaagttatg
    16021 aatcccacat gtaagaagcg tttgggtgtc attcggttgg aaaccatgaa ggtggttgca
    16081 caagaggaaa tcgcgccagc aatctttgaa ttagtcctag aaggagaaat ggttgaagcc
    16141 atgcgagcag gccaatttct tcatctgcgt gtaccagacg atgcccatct tttacgtcgt
    16201 cctatttcaa tttcgtctat tgacaaggca aacaagcagt gtcacctcat ttatcggatt
    16261 gagggagctg ggactgcaat tttttcaacc ttaagtcagg gagacactct tgatgtgatg
    16321 gggcctcagg gaaatggttt tgacttgtct gaccttgatg agcagaatca ggttctcctt
    16381 gttggtggtg ggattggtgt tccacccttg cttgaggtgg ccaaggaatt gcatgaacgt
    16441 ggagtgaaag tagtgacagt cctcggtttt gctaataagg atgctgttat tttgaaaacg
    16501 gaattggctc agtatggtca ggtctttgta acgacagatg atggttctta tggcatcaag
    16561 ggaaatgttt ccgttgttat caatgattta gatagtcagt ttgatgctgt ttactcgtgt
    16621 ggggctccag gaatgatgaa gtatatcaat caaacctttg atgatcaccc aagagcctat
    16681 ttatctctgg aatctcgtat ggcttgtggg atgggagctt gctatgcctg tgttctaaaa
    16741 gtaccagaaa gcgagacggt caaccaacgc gtctgtgaag atggtcctgt tttccgcaca
    16801 ggaacagttg tattataagg agaaaattat gactacaaat cgattacaag tgtctctacc
    16861 tggtttggat ttgaaaaatc cgattattcc agcatcaggt tgttttggct ttggacaaga
    16921 gtatgccaag tactatgatt taaacctttt aggttctatt atgatcaagg cgacaaccct
    16981 tgaaccacgt tttgggaacc caactccaag agtagcagag acgcctgctg gtatgctcaa
    17041 tgcaattggc ttgcaaaatc ctggtttaga ggttgttttg gctgaaaagc taccttggct
    17101 ggaaagagaa tatccaaatc ttcctattat tgccaatgta gctggttttt caaaacaaga
    17161 gtatgcagct gtttctcatg ggatttccaa ggcaactaat gtaaaagcta tcgagctcaa
    17221 tatttcttgt cccaatgttg accactgtaa tcatggactt ttgattggtc aagatccaga
    17281 tttggcttat gatgtggtga aagcagctgt ggaagcctca gaagtgccag tttatgtcaa
    17341 attaaccccg agtgtgaccg atatcgttac tgtcgcaaaa gctgcagaag atgcgggagc
    17401 aagtggcttg accatgatca atactctggt tggaatgcgc tttgacctca aaaccagaaa
    17461 accaatcttg gccaatggaa caggtggaat gtcaggtcca gcagtctttc cagtagccct
    17521 caaactcatc cgccaagtag cccaaacaac aaacctgcct atcattggaa tggggggagt
    17581 ggattcggct gaagctgccc tagaaatgta tctggctggg gcatctgcta tcggagttgg
    17641 aacagctaac tttaccaatc cttatgcctg ccctgacatc atcgaaaatt taccaaaagt
    17701 catggataaa tacggtatta gcagtctgga agaactccgt caggaagtaa aagagtctct
    17761 gaggtaaact gcaatcaatc tgttcttgat tttttattag tttgtaatat gaatttagga
    17821 gaattttggt acaataaaat aaataagaac agaggaagaa ggttaatgaa gaaagtaaga
    17881 tttatttttt tagctctgct atttttctta gctagtccag agggtgcaat ggctagtgat
    17941 ggtacttggc aaggaaaaca gtatctgaaa gaagatggca gtcaagcagc gaatgagtgg
    18001 gtttttgata ctcattatca atcttggttc tatataaaag cagatgctaa ctatgctgaa
    18061 aatgaatggc taaagcaagg tgacgactat ttttacctca aatctggtgg ctatatggcc
    18121 aaatcagaat gggtagaaga caagggagcc ttttattatc ttgaccaaga tggaaagatg
    18181 aaaagaaatg cttgggtagg aacttcctat gttggtgcaa caggtgccaa agtaatagaa
    18241 gactgggtct atgattctca atacgatgct tggttttata tcaaagcaga tggacagcac
    18301 gcagagaaag aatggctcca aattaaaggg aaggactatt atttcaaatc cggtggttat
    18361 ctactaacaa gtcagtggat taatcaagct tatgtgaatg ctagtggtgc caaagtacag
    18421 caaggttggc tttttgacaa acaataccaa tcttggtttt acataaaaga aaatggaaac
    18481 tatgctgata aagaatggat tttcgagaat ggtcactatt attatctaaa atctggtggc
    18541 tacatggcag ccaatgaatg gatttgggat aaggaatctt ggttttatct caaatttgat
    18601 gggaaaatag ctgaaaaaga atgggtctac gattctcata gtcaagcctg gtactacttc
    18661 aaatctggtg gttacatggc gaaaaatgag acagtagatg gttatcagct tggaagcgat
    18721 ggtaaatggc ttggaggaaa agctacaaat gaaaatgctg cttactatca agtagtgcct
    18781 gttacagcca atgtttatga ttcagatggt gaaaagcttt cctatatatc gcaaggtagt
    18841 gtcgtatggc tagataagga tagaaaaagt gatgacaagc gcttggctat tactatttct
    18901 ggtttgtcag gctatatgaa aacagaagat ttacaagcgc tagatgctag taaggacttt
    18961 atcccttatt atgagagtga tggccaccgt ttttatcact atgtggctca gaatgctagt
    19021 atcccagtag cttctcatct ttctgatatg gaagtaggca agaaatatta ttcggcagat
    19081 ggcctgcatt ttgatggttt taagcttgag aatcccttcc ttttcaaaga tttaacagag
    19141 gctacaaact acagtgctga agaattggat aaggtattta gtttgctaaa cattaacaat
    19201 agccttttgg agaacaaggg cgctactttt aaggaagccg aagaacatta ccatatcaat
    19261 gctctttatc tccttgccca tagtgcccta gaaagtaact ggggaagaag taaaattgcc
    19321 aaagataaga ataatttctt tggcattaca gcctatgata cgacccctta cctttctgct
    19381 aagacatttg atgatgtgga taagggaatt ttaggtgcaa ccaagtggat taaggaaaat
    19441 tatatcgata ggggaagaac tttccttgga aacaaggctt ctggtatgaa tgtggaatat
    19501 gcttcagacc cttattgggg cgaaaaaatt gctagtgtga tgatgaaaat caatgagaag
    19561 ctaggtggca aagattagta ctataagtga atatgatttg agtgaatagt aagttaaaaa
    19621 tcctgatttc aagtaaaatc aggatttttt catggatgca atttttttgg agtctggtgt
    19681 gacgcggagg gtcttttgtc ctgtgtaagt gacaaagccg ggttttccac cagttggttt
    19741 attgagtttt ttgacttcaa tcatatctac ctgcaccaga ttcgacaggc gcccttgaga
    19801 gaagtaggca gctaactctg ctgcgtctgt cttgactgca tcagatgggt caagatttcc
    19861 tgagatgaca acatggcttc caggaatgtc cttagcatgg aaccaaagtt cctccttgcg
    19921 ggccatttta aaggtcaatt cctcattttg aagattgttt cgtccgacat agatgatggt
    19981 tttgccatcg cttgctagat attgttctag ttttttgcgt ttctggattt tctcccgttg
    20041 tcttctgcgg ataaaacctg tttgaatcaa ttcttcacgg atttcagcga tttcttccag
    20101 tccagcttgg ttgaggacgg tttctacact ttccagatag agaatagtgg ctttggtttc
    20161 ttcaatcaaa tcagtcaagt atttgacagc ttctttgagt ttctgatacc gtttaaaata
    20221 gcgttgggca ttctggttgg gagtcagagc cttatcaagc gcaatcatga taggttggtt
    20281 ggtatagtag ttgtctagga taacctggtc ttggtcgtta ggcacttggt ggaggaaggt
    20341 tgtcagcaat tctccttttt gacgaaattc ttcagcgttg tctgtcgcca gtaactcttt
    20401 ttcctgtttt ttgagtttgt gtcggttttt ctgaagttca ttttcaacac gacgaatcag
    20461 ttcactggcc tgctgtttga cgcggtcgcg ctcagcctta tccttatagt aggtgtccaa
    20521 caaatcagaa agatttgcaa gaggctctcc cacctgattt gcaaaaggaa ctggactgaa
    20581 ggaagtctca gtcaagcatg gcttggtttc ttgattgaaa aaatttcgga aagcggaaag
    20641 tttttcacta accagtatcc tttccaattc atttgccgta tcgcgtccca gaccttgaaa
    20701 gaggctttga agattttttg ctgttagttc ttgggtttgc aggatttcaa agagcttttc
    20761 atccttgata gtaaaaggat tgagagattt tgtacttggc ggagcgatat aggtcgatcc
    20821 tggaagtaag gtgcggtagc tattttgtga aaagccgacg tgtttgataa cttcgaggat
    20881 tttatgactg cttttatcga ccagtagaat attactgtgt ttccccataa tttcgataat
    20941 caaggtagcc tggatatggt ctccaatctc gtttttattg gaaactgtaa tttccacaat
    21001 acggtcattt tccacttgct caatcgactc aatcagggcc ccctgcaaat actttctcaa
    21061 aaccatgata aaggtagaag gttgggctgg attttcaaaa gtcgtttggg tcagctgaat
    21121 gcgtccaaaa actggatggg cagaaaggag caggcgatgg ctttggcgat tgctgcggat
    21181 ttgcaagacc aactcttgtt caaaaggctg attgattttc tggatgcgac cattcactaa
    21241 ttcgcttcgc aattcctcaa ctatgtggtg taaaaaaaat ccgtcaaatg acatcgttct
    21301 ctccttgtga ttgtattcca tagtattata tcaaaaaggt agaataaaat catggaaatg
    21361 tggtataata aagccaagta aagagaaacg agaagcacat gtatattgaa atggtagatg
    21421 aaactggtca agtttcaaaa gaaatgttgc aacaaaccca agaaattttg gaatttgcag
    21481 cccaaaaatt aggaaaagaa gacaaggaga tggcagtcac ttttgtgacc aatgagcgta
    21541 gtcatgaact taatctgggg taccgtgaca ccgaccgtcc gacagatgtc atcagccttg
    21601 agtataaacc agaattggaa attgcctttg acgaagagga tttgcttgaa aatccagaat
    21661 tggcagagat gatgtctgag tttgatgcct atattgggga attgttcatc tctatcgata
    21721 aggctcatga gcaggccgaa gaatatggtc acagctttga gcgtgagatg ggcttcttgg
    21781 cagtacacgg ctttttacat attaacggct atgatcacta tactccggaa gaagaagcgg
    21841 agatgttcgg tttacaagaa gaaattttga cagcctatgg actcacaaga caataaacga
    21901 aaatggaaaa atcgtgacct gatatccagt ttagaatttg ccatcacagg gatttttact
    21961 gctatcaagg aagaacgcaa tatgcgaaaa catgcagtga cggctctagt ggtcatcctt
    22021 gcaggttttg gttttcaggt gtcacgaatc gaatggctct ttctcctatt gagcattttc
    22081 ttggtggtag cctttgaaat tatcaattct gctatcgaaa atgtggtgga tttggccagt
    22141 cactatcact tttccatgct ggctaaaaat gccaaggata tggcggctgg cgcggtatta
    22201 gtggtttctc tttttgcagc cttaacaggc gcattgattt ttctcccacg aatctgggat
    22261 ttattatttt aaacagtaag aggaaattat gacttttaaa tcaggctttg tagccatttt
    22321 aggacgtccc aatgttggga agtcaacctt tttaaatcac gttatggggc aaaagattgc
    22381 catcatgagt gacaaggcgc agacaacgcg caataaaatc atgggaattt acacgactga
    22441 taaggagcaa attgtcttta tcgacacacc agggattcac aaacctaaaa cagctctcgg
    22501 agatttcatg gttgagtctg cctacagtac ccttcgcgaa gtggacactg ttcttttcat
    22561 ggtgcctgct gatgaagcgc gtggtaaggg ggacgatatg attatcgagc gtctcaaggc
    22621 tgccaaggtt cctgtgattt tggtggtgaa taaaatcgat aaggtccatc cagaccagct
    22681 cttgtctcag attgatgact tccgtaatca aatggacttt aaggaaattg ttccaatctc
    22741 agcccttcag ggaaataacg tgtctcgtct agtggatatt ttgagtgaaa atctggatga
    22801 aggtttccaa tatttcccgt ctgatcaaat cacagaccat ccagaccgtt tcttagtttc
    22861 agaaatggtt cgcgagaaag tcttgcacct aactcgtgaa gagattccgc attctgtagc
    22921 agtagttgtt gactctatga aacgagacga agagacagac aaggttcaca tccgtgcaac
    22981 catcatggtc gagcgcgata gccaaaaagg gattatcatc ggtaaaggtg gcgctatgct
    23041 taagaaaatc ggtagcatgg cccgtcgtga tatcgaactc atgctaggag acaaggtctt
    23101 cctagaaacc tgggtcaagg tcaagaaaaa ctggcgcgat aaaaagctag atttggctga
    23161 ctttggctat aatgaaagag aatactaagt agaggtgggc tcatgcctgc ttcttgtttt
    23221 tacagaagga ggacttatgc ctgaattacc tgaggttgaa accgtttgtc gttgcttaga
    23281 aaaattgatt ataggaaaga agatttcgag tatagaaatt cgctacccca agatgattaa
    23341 gacggatttg gaagagtttc aaagggaatt gcctagtcag attatcgagt caatgggacg
    23401 tcgtggaaaa tatttgcttt tttatctgac agacaaggtt ttgatttccc atttgcggat
    23461 ggagggcaag tatttttact atccagacca aggacctgaa cgcaagcatg cccatgtttt
    23521 ctttcatttt gaagatggtg gcacgcttgt ttatgaggat gttcgcaagt ttggaaccat
    23581 ggaactcttg gtgcctgacc ttttagacgc ctactttatt tctaaaaaat taggtcctga
    23641 accaagcgaa caagactttg atttacaggt ctttcaatct gcccttgcca agtccaaaaa
    23701 gcctatcaaa tcccatctcc tagaccagac cttggtagct ggacttggca atatctatgt
    23761 ggatgaggtt ctctggcgag ctcaggttca tccagctaga tcttcccaga ctttgacagc
    23821 agaagaagcg acagctattc atgaccaaac cattgctgtt ttgagccagg ctgttgaaaa
    23881 aggcggttcc accattcgga cctataccaa tgcctttggg gaagatggaa gcatgcagga
    23941 ttttcatcag gtctatgata agactggtca aggatgtgta cgctgtggta ccatcattga
    24001 gaaaattcaa ctaggtggac gtggaaccca cttttgtcca aactgtcaaa ggagggactg
    24061 atgggaaaaa tcatcggaat cactggggga attgcctctg gtaagtcaac tgtgacaaat
    24121 tttctaagac agcaaggctt tcaagtagtg gatgccgacg cagtcgtcca ccaactacag
    24181 aaacctggtg gtcgtctgtt tgaggctcta gtacagcact tttggcaaga aatcattctt
    24241 gaaaacggag aactcaatcg ccctctccta gctagtctca tcttttcaaa tcctgatgaa
    24301 cgagaatggt ctaagcaaat tcaaggggag attatccgtg aggaactggc tactttgaga
    24361 gaacagttgg ctcagacaga agagattttc ttcatggata ttcccctact ttttgagcag
    24421 gactacagcg attggtttgc tgagacttgg ttggtctatg tggaccgaga tgcccaagta
    24481 gaacgcttaa tgaaaaggga ccagttgtcc aaagatgaag ctgagtctcg tctggcagcc
    24541 cagtggcctt tagaaaaaaa gaaagatttg gccagccagg ttcttgataa taatggcaat
    24601 cagaaccagc ttcttaatca agtgcatatc cttcttgagg gaggtaggca agatgacaga
    24661 gattaactgg aaggataatc tgcgcattgc ctggtttggt aattttctga caggagccag
    24721 tatttctttg gttgtacctt ttatgcctat cttcgtggaa aatctaggtg tagggagtga
    24781 gcaagtcgct ttttatgcag gcttagcaat ttctgtctct gctatttccg cggcgctctt
    24841 ttctcctatt tggggtattc ttgctgacaa atacggccga aaacccatga tgattcgggc
    24901 aggtcttgct atgactatca ctatgggagg cttggccttt gtcccaaata tctattggtt
    24961 aatctttctt cgtttactaa acggtgtatt tgcaggtttt gttcctaatg caacggcact
    25021 gatagccagt caggttccaa aggagaaatc aggctctgcc ttaggtactt tgtctacagg
    25081 cgtagttgca ggtactctaa ctggtccctt tattggtggc tttatcgcag aattatttgg
    25141 cattcgtaca gttttcttac tggttggtag ttttctattt ttagctgcta ttttgactat
    25201 ttgctttatc aaggaagatt ttcaaccagt agccaaggaa aaggctattc caacaaagga
    25261 attatttacc tcggttaaat atccctatct tttgctcaat ctctttttaa ccagttttgt
    25321 catccaattt tcagctcaat cgattggccc tattttggct ctttatgtac gcgatttagg
    25381 gcagacagag aatcttcttt ttgtctctgg tttgattgtg tccagtatgg gcttttccag
    25441 catgatgagt gcaggagtca tgggcaagct aggtgacaag gtgggcaatc atcgtctctt
    25501 ggttgtcgcc cagttttatt cagtcatcat ctatctcctc tgtgccaatg cctctagccc
    25561 ccttcaacta ggactctatc gtttcctctt tggattggga accggtgcct tgattcccgg
    25621 ggttaatgcc ctactcagca aaatgactcc caaagccggc atttcgaggg tctttgcctt
    25681 caatcaggta ttcttttatc tgggaggtgt tgttggtccc atggcaggtt ctgcagtagc
    25741 aggtcaattt ggctaccatg ctgtctttta tgcgacgagc ctttgtgttg cctttagttg
    25801 tctctttaac ctgattcaat ttcgaacatt attaaaagta aaggaaatct agtgcgagta
    25861 aaaattaatc tcaaatgctc ctcttgtggc agtatcaatt acctaaccag taaaaattca
    25921 aaaacccatc cagacaagat tgaggtttta aagtattgtc ccaaggaaag aaaagtaacc
    25981 ctacatcttg aatctaagta gattttatgg taaaataata gggattttaa ggagtttgat
    26041 atgtataacc tattattaac cattttatta gtattatctg ttgtgattgt gattgcaatt
    26101 ttcatgcaac caaccaaaaa ccaatccagc aatgtatttg atgccagttc aggtgatttg
    26161 tttgaacgta gtaaagctcg cggttttgaa gctgtaatgc agcgtttgac agggatttta
    26221 gtctttttct ggctagccat tgccttagca ttgacggtat tatcaagtag ataagaaaat
    26281 aatgggcagg actaggtctt tgcctctttt tatttttaaa ggatgtttga gaaggtttta
    26341 cagtaaaaga aaattaaaaa atctagaaag aaaatatgaa agatagaata aaagaatatt
    26401 tacaagacaa gggaaaggtg actgttaatg atttggctca ggctttggga aaagacagtt
    26461 ccaaggattt tcgtgagttg attaaaacct tgtccttaat ggaaagaaag caccaaattc
    26521 gttttgaaga agatggtagt ctgacattag aaattaagaa aaaacatgag attaccctca
    26581 aggggatttt tcatgcccat aaaaatggct ttggctttgt tagtctggaa ggcgaggagg
    26641 acgacctttt tgtagggaaa aatgatgtca actatgctat tgatggtgat accgtcgagg
    26701 tagtgattaa gaaagtcgct gaccgcaata agggaacagc agcagaagcc aaaattattg
    26761 atatcctaga acacagtttg acaacagttg tcgggcaaat cgttctggat caggaaaaac
    26821 ctaagtatgc tggctatatt cgttcaaaaa atcagaaaat cagtcaaccg atttatgtta
    26881 agaaaccagc cctaaaatta gaaggaacag aagttctcaa agtctttatc gataaatacc
    26941 caagcaagaa acatgatttc tttgtcgcga gtgttctcga tgtagtggga cactcaacgg
    27001 atgtcggaat tgatgttctt gaggtcttgg aatcaatgga cattgtatcc gagtttccag
    27061 aagctgttgt taaggaagca gaaagtgtgc ctgatgctcc gtctcaaaag gatatggaag
    27121 gtcgtctgga tctaagagat gaaattacct ttaccattga cggtgcggat gccaaggact
    27181 tggacgatgc agtgcatatc aaggctctga aaaatggcaa tctggagctt ggggttcaca
    27241 tcgcagatgt ttcttattat gtgaccgagg ggtctgccct tgacaaggaa gcccttaacc
    27301 gtgcgacttc tgtttacgtg acagaccgag tggtgccaat gcttccagaa cgactatcaa
    27361 atggcatctg ctctctcaat ccccaagttg accgcctgac ccagtctgct attatggaga
    27421 ttgataaaca tggtcgtgtg gtcaactata ccattacaca aacagttatc aagaccagtt
    27481 ttcgtatgac ctatagcgat gtcaatgata tcctagctgg cgatgaagaa aagagaaaag
    27541 aatatcataa aattgtatca agtatcgaac tcatggccaa gcttcatgaa actttagaaa
    27601 acatgcgtgt gaaacgtgga gctctcaatt ttgataccaa tgaagcgaag attttagtgg
    27661 ataaacaagg taagcctgtt gatatcgttc ttcggcagcg tggtattgcc gagcggatga
    27721 ttgagtcttt tatgttgatg gctaatgaaa cagttgccga acatttcagc aagttggatt
    27781 tgccttttat ctatcgaatt cacgaggagc ctaaggctga aaaggttcag aagtttattg
    27841 attatgcttc gagttttggc ttgcgcattt atggaactgc cagtgagatt agtcaggagg
    27901 cacttcaaga catcatgcgt gctgttgagg gagaacctta tgcagatgta ttgtccatga
    27961 tgcttcttcg ctctatgcag caggctcgtt attcggagca caatcacggc cactatggac
    28021 tagctgctga ctattatact cactttacca gtccaattcg tcgttatcca gaccttcttg
    28081 ttcaccgtat gattcgggat tacggccgtt ctaaggaaat agcagagcat tttgaacaag
    28141 tgattccaga gattgcgacc cagtcttcca accgtgaacg tcgtgccata gaagctgagc
    28201 gtgaagtcga agccatgaaa aaggctgagt atatggaaga atacgtgggt gaagagtatg
    28261 atgcagttgt atcaagtatt gtcaaattcg gtctctttgt cgaattgcca aacacagttg
    28321 aaggcttgat tcacatcact aatctacctg aattttatca tttcaatgag cgtgatttga
    28381 ctcttcgtgg agaaaaatca ggtatcactt tccgagtggg tcagcagatc cgtatccgtg
    28441 ttgaaagagc ggataaaatg actggagaga ttgatttttc attcgtacct agtgagtttg
    28501 atgtgattga aaaaggcttg aaacagtcta gtcatagtgg cagagggcgt ggttcaaatc
    28561 gtcgttcgga taagaaggaa gacaagagaa aatcaggacg ctcaaatgat aagcgtaagc
    28621 attcacaaaa agacaagaag aaaaaaggaa agaaaccttt ttacaaggaa gtagctaaga
    28681 aaggagccaa gcatggcaaa gggcgaggga aaggtcgtcg cacaaaataa aaaggcacgc
    28741 cacgactata caatcgtaga tacgctagag gcagggatgg tcctgactgg aactgaaatc
    28801 aagagtgtac gagctgctcg aattaatatc aaggatggct ttgctcaagt gaaaaatgga
    28861 gaagtttggc tgagcaatgt tcatatcgcg ccttacgaag agggcaatat ctggaaccag
    28921 gaaccagaac gtcgtcgtaa actcctgctc cataaaaagc aaattcaaaa attggaacaa
    28981 gagaccaaag ggacaggaat gaccttagtt ccccttaagg tctatataaa agatggctac
    29041 gctaagcttc ttttaggact tgccaaaggg aagcatgact atgacaaacg ggagtctatc
    29101 aaacgtcgtg agcaaaatcg agatatcgcg cgtgtgatga aagctgttaa tcagcgataa
    29161 aaagaggaat tgaaaatgga aaaattagtt gcctataaac gcatgccttt gtggaataaa
    29221 caaacaatgc ctgaagatgt tcagcaaaag cacaatacaa aagttgggac ttgggggaaa
    29281 attactgtct tgaagggagc tctcaagttt attgaattga cagaagaagg ggaagttcta
    29341 gctgaacacc tctttgaagc aggggcagac aatccaatgg cccaacctca agcctggcac
    29401 cgagtggaag ctgccacaga tgatgtggaa tggtacttgg aattttattg taaacctgag
    29461 gattattttg ctaaaaaata caataccaat cctgttcatt cagaggtcct agaggccatg
    29521 cagacagtga aacaagggaa agctttggat ttgggttgtg gtcaggggcg taattctctt
    29581 tttctagccc agcaagattt tgatgtgacg gctgtagatc aaaatggact agctcttgaa
    29641 atcttgcaaa gcattgtgga gcaggaagat ttggacatgc ctgttggcct ttacgatatc
    29701 aattcagcta gcattgaaca agaatatgat tttatcgttt caacagttgt tctcatgttt
    29761 ctacaagcgg accgcattcc agctattatt caaaatatgc aggagaaaac cagtgttggt
    29821 ggttacaacc ttatcgtttg tgccatggac acggaggatt atccttgctc ggttaatttc
    29881 ccattcacct ttaaagaagg agaactggca gactattaca aggattggga attggttaag
    29941 tacaatgaaa atccaggcca tttgcaccgt cgcgatgaga atggcaatcg tattcaacta
    30001 cgctttgcga ccttactagc taagaaaatc aagtaaacac acatgaagat taggaatttt
    30061 cctgatcttt tttctttttt acgaatgata tagaaaagga gggaattcat gtttgttgcg
    30121 agagatgcta ggggagaatt ggtaaatgtg ttagaggata aacttgagaa gcaagcatac
    30181 acctgcccag cttgtggagg ccagctccat ttgcgtcaag gaccaagtgt acggacgcat
    30241 tttgcccata aatccttaaa agactgtgat tttttctttg aaaatgaaag tccagaacac
    30301 ctggccaata aggaatccct ctatcactgg ttgaaaaaag agacaaaggt tcaattagag
    30361 tacccgcttt cagaacttaa acagattgcg gatgtatttg taaatggcaa tctagctcta
    30421 gaagttcagt gtagtccctt gcctcagaaa gtccttaaag agcgaagtga gggatatcgt
    30481 agtcagggtt accaagtact gtggttgctg ggtcaaaaac tgtggctcaa ggagcgtttg
    30541 actcgtctac agcaaggttt tctttatttc agtcaaaaca tgggctttta tgtttgggaa
    30601 ttagacaagg aaaaacaagt tttaagactc aaatacctga tttaccagga tctccgcggt
    30661 aaactccatt atcaaatcaa ggaattttcc tatggtcaag gtagtttatt ggaaatattg
    30721 cgtcttccct ataagaaaca aaaaatatct cattttacag tttctgagga caaggacatc
    30781 tgtcgctata tccggcaaca actttattat caaaatctct tttggatgaa agaacaagca
    30841 gaagcctatc aaaagggaga aaatatcctg acttatggac tgaaagaatg gtatccacaa
    30901 attcgaccaa tagtgggcaa atttttccag attgaacaag acttgactag ctattatcag
    30961 cacttttata cctattacca aaaaaatcct caaaatgatt ggcaaaagct ttatccacca
    31021 gccttttatc agcaatattt cttgaaaaat atggtagaat agaaaggatg gaggaatcta
    31081 atggtattac aaagaaatga aataaatgaa aaagatacat gggatctatc aacgatctac
    31141 ccaactgacc aggcttggga agaagtctta aaagatttaa cagaacaatt ggagacagta
    31201 gcccagtatg aaggccatct cttggatagt gcggataacc tactagaaat cactgaattt
    31261 tctcttgaaa tggaacgcca gatggagaag ctttacgttt atgctcatat gaagaatgac
    31321 caggatacac gtgaagctaa gtatcaagag tactatgcca aggccatgac attctacagc
    31381 cagttagacc aagccttttc attctatgag cctgaattta tggagattag cgaaaagcag
    31441 tatgctgact ttttagaagc tcaaccaaag ctgcaggttt atcaacacta ttttgacaag
    31501 ctcttgcaag gcaaggatca cgttctctca caacgtgaag aagaattatt ggctggagct
    31561 ggagaaatct ttggttcagc aagtgaaacc ttcgctatct tggacaatgc ggatattgtg
    31621 ttcccttatg tcctagacga tgatggtaaa gaagttcagc tatctcatgg gatttacaca
    31681 cgtttgatgg agtctaaaaa acgtgaggtt cgccgtggtg cctatcaagc tctttatgcg
    31741 acttacgaac aattccaaca cacctatgcc aaaaccttgc aaaccaatgt taaggtgcaa
    31801 aattaccgtg ctaaagttcg taactacaag agtgctcgtc atgcagccct cgcagcgaat
    31861 tttgttccag aaagtgttta tgacaatttg gtagcagcag ttcgcaagca tttgccactc
    31921 ttacatcgct atcttgagct tcgttcaaaa atcttgggga tttcagatct caagatgtac
    31981 gatgtctaca caccgctttc atctgttgaa tacagtttta cctaccaaga agccttgaaa
    32041 aaagcagaag atgctttggc agtcttgggt gaggattact tgagccgtgt taaacgtgcc
    32101 ttcagcgagc gttggattga tgtttacgaa aatcaaggca agcgttcagg tgcctactct
    32161 ggtggttctt acgataccaa tgcctttatg cttctcaact ggcaggacaa tctggacaat
    32221 ctctttactc ttgttcatga aacaggtcac agtatgcatt caagctatac tcgtgaaact
    32281 cagccttatg tttacggaga ttactctatc tttttggctg agattgcctc aactaccaat
    32341 gaaaatatct tgacggagaa attattggaa gaagtggaag acgacgcaac acgctttgct
    32401 attctcaata acttcctaga tggtttccgt ggaacagttt tccgccaaac tcaatttgct
    32461 gagtttgaac acgccattca ccaagcagat caaaatgggg aggtcttgac aagcgatttc
    32521 ctaaataaac tctacgcaga cttgaaccaa gagtattatg gtttgagtaa ggaagacaat
    32581 cctgaaatcc aatacgagtg ggctcgcatt ccacacttct actataacta ctatgtatat
    32641 caatattcaa ctggctttgc ggccgcctca gccttggctg aaaaaattgt ccatggtagt
    32701 caagaagacc gtgaccgcta tatcgactac ctcaaggcag gtaagtcgga ctatccactt
    32761 aatgtcatga gaaaagctgg tgttgatatg gagaaggaag actacctcaa cgatgccttt
    32821 gcagtctttg aacgccgttt aaatgagttt gaagcccttg ttgaaaaatt aggattggca
    32881 taaaatggtt gaatcgtata gtaagaatgc taaccataac atgcgtcgtc ctgtcgtcaa
    32941 agaagaaatt gtagacttga tgcgtcagcg tcaaaagcag gtcacaggtt tcttgaaaga
    33001 attggaaaac tttgcccgca aggaaaatat tcctattatt ccccatgaaa cggttgctta
    33061 tttccgtttt cttatggaaa ccatgcagcc taaaaatatt ctggaaattg ggacggctat
    33121 cggtttttca gctctcttga tggctgaaca tgcgccaaat gctaagatta caactattga
    33181 tcgtaatcca gaaatgattg gttttgccaa ggaaaatttt gcccagtttg acagtcgcaa
    33241 gcaaatcact ctcctagagg gagatgcggt ggatgtctta tctacactga cagagtctta
    33301 tgatttcgtc tttatggatt ctgccaagtc taaatacatc gtctttctgc cagaaatcct
    33361 caaacatttg gaagttggtg gtgtggttgt cttggatgat atttttcaag gtggtgatgt
    33421 tgccaaggat attatggaag tccgtcgtgg tcagcgaacc atttatcgag gccttcaaaa
    33481 attatttgat gcaaccttag acaatccaga actcaccgca acattagtgc ctttaggaga
    33541 tggtattctc atgcttcgta aaaatgtagc agatgttcaa ctgtctgaaa gcgaatgatt
    33601 ttcagaaaaa tttaagaaaa aatagtaaaa tagatagagt aacacttatc tcaaaggagt
    33661 agacatgaag aaaaaattat tggcaggtgc catcacacta ttatcagtag caactttagc
    33721 agcttgttcg aaagggtcag aaggggcaga ccttatcagc atgaaagggg atgtcatcac
    33781 agaacatcaa ttttatgagc aagtgaaaaa caacccttca gcccaacaag tcttgttaaa
    33841 tatgaccatc caaaaagttt ttgaaaaaca atatggctca gagcttgatg ataaagaggt
    33901 tgatgatact attgccgaag aaaaaaaaca atatggcgaa aactaccaac gtgtcttgtc
    33961 acaagcaggt atgactcttg aaacacgtaa agctcaaatt cgtacaagta aattagttga
    34021 gttggcagtt aagaaggtag cagaagctga attgacagat gaagcctata agaaagcctt
    34081 tgatgagtac actccagatg taacggctca aatcatccgt cttaataatg aagataaggc
    34141 caaagaagtt ctcgaaaaag ccaaggcaga aggtgctgat tttgctcaat tagccaaaga
    34201 taattcaact gatgaaaaaa caaaagaaaa tggtggagaa attacctttg attctgcttc
    34261 aacagaagta cctgagcaag tcaaaaaagc cgctttcgct ttagatgtgg atggtgtttc
    34321 tgatgtgatt acagcaactg gcacacaagc ctacagtagc caatattaca ttgtaaaact
    34381 cactaagaaa acagaaaaat catctaatat tgatgactac aaagaaaaat taaaaactgt
    34441 tatcttgact caaaaacaaa atgattcaac atttgttcaa agcattatcg gaaaagaatt
    34501 gcaagcagcc aatatcaagg ttaaggacca agccttccaa aatatcttta cccaatatat
    34561 cggtggtgga gattcaagct caagcagtag tacatcaaac gaatagtcca aatcaatgag
    34621 tcagggaaaa aaactcgact tcaggaaaaa atgaagcaaa cattcccaca ataaaacgca
    34681 tagtacaagg tttgtactga ccccaaaagt tagacaatta atttatctga aggatttagt
    34741 tctgtattgc acagggctaa gtccttttag ttttacctta attctcttat tgttgtaata
    34801 atcaatatag tctataatgg cttgttccaa ttgattaagt gatttaaatg ttttctcata
    34861 gccataaaac atttcggatt ttaaaatgcc aaagaaagat tccatcctac cgttgtcttg
    34921 gctgtttcct ttgcgtgaca tagatgcttg aattccctta ctctctagga agcgatgata
    34981 agaatcgtgt tg
//
LOCUS       DAVBCW010000009        34038 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVBCW010000009 DAVBCW010000000
VERSION     DAVBCW010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34038
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.009"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(167..1153)
                     /locus_tag="VQ514_000392"
     CDS             complement(167..1153)
                     /locus_tag="VQ514_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2499208.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            complement(1163..1963)
                     /locus_tag="VQ514_000393"
     CDS             complement(1163..1963)
                     /locus_tag="VQ514_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HET2499209.1"
                     /translation="MLPYPFSYFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTASQAPSVVIG
                     PSQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRIYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            complement(2216..3058)
                     /locus_tag="VQ514_000394"
     CDS             complement(2216..3058)
                     /locus_tag="VQ514_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2499210.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            complement(3060..4352)
                     /locus_tag="VQ514_000395"
     CDS             complement(3060..4352)
                     /locus_tag="VQ514_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2499211.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            complement(4463..5734)
                     /locus_tag="VQ514_000396"
     CDS             complement(4463..5734)
                     /locus_tag="VQ514_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2499212.1"
                     /translation="MSSKFMKSTAVLGTATLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIAGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            6284..7801
                     /gene="malQ"
                     /locus_tag="VQ514_000397"
     CDS             6284..7801
                     /gene="malQ"
                     /locus_tag="VQ514_000397"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HET2499213.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLGASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEHFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            7827..10085
                     /gene="glgP"
                     /locus_tag="VQ514_000398"
     CDS             7827..10085
                     /gene="glgP"
                     /locus_tag="VQ514_000398"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HET2499214.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLKSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIELDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(11130..11702)
                     /locus_tag="VQ514_000399"
     CDS             complement(11130..11702)
                     /locus_tag="VQ514_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499215.1"
                     /translation="MDKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHVAKSAIRSAISRYLGSWFVNDVALE
                     FAMNLLSPGTYLAQLWDKNDAIPNNGRINF"
     gene            complement(12324..13172)
                     /locus_tag="VQ514_000400"
     CDS             complement(12324..13172)
                     /locus_tag="VQ514_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET2499216.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYNKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            13324..13602
                     /locus_tag="VQ514_000401"
     CDS             13324..13602
                     /locus_tag="VQ514_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499217.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            13604..15667
                     /locus_tag="VQ514_000402"
     CDS             13604..15667
                     /locus_tag="VQ514_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET2499218.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIEFIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIKLMKKSEENKKTKQRYFIKMADKVVKYNFFGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWFSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(15745..17001)
                     /locus_tag="VQ514_000403"
     CDS             complement(15745..17001)
                     /locus_tag="VQ514_000403"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HET2499219.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKVFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            17143..19608
                     /locus_tag="VQ514_000404"
     CDS             17143..19608
                     /locus_tag="VQ514_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HET2499220.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGAGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVAGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNLTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGALATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            19673..20584
                     /locus_tag="VQ514_000405"
     CDS             19673..20584
                     /locus_tag="VQ514_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET2499221.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            20814..21512
                     /gene="dapD"
                     /locus_tag="VQ514_000406"
     CDS             20814..21512
                     /gene="dapD"
                     /locus_tag="VQ514_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HET2499222.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVCVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            21580..22710
                     /locus_tag="VQ514_000407"
     CDS             21580..22710
                     /locus_tag="VQ514_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HET2499223.1"
                     /translation="MLDLIQTRRALHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFTSQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDGAFGDWLPNQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            22722..23261
                     /locus_tag="VQ514_000408"
     CDS             22722..23261
                     /locus_tag="VQ514_000408"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HET2499224.1"
                     /translation="MKSELRKQVLHEMKAISQEQKQAIDQALTERILQHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLTPKTYPKGRMDFVVYDPQQLVKTSFALLEPQ
                     GDLEVVDASQIDLIHVPGLAFTTDGYRIGYGGGYYDRYLEHFSGRALSTIYPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            23245..23922
                     /locus_tag="VQ514_000409"
     CDS             23245..23922
                     /locus_tag="VQ514_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET2499225.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(23994..24893)
                     /gene="galU"
                     /locus_tag="VQ514_000410"
     CDS             complement(23994..24893)
                     /gene="galU"
                     /locus_tag="VQ514_000410"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HET2499226.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDKTTDMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FQILENQAPGAGNEIQLTDAIDTLNKTQRVFAREFNGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(24915..25931)
                     /locus_tag="VQ514_000411"
     CDS             complement(24915..25931)
                     /locus_tag="VQ514_000411"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HET2499227.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     YHTNKHYFKDVVLDENIIAYTDLAETLKDVDAILFVVPTKVTRLVAQQVAQTLDHKAI
                     IMHASKGLEPDSHKRLSTILEEEIPEQLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQELFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            26143..26523
                     /locus_tag="VQ514_000412"
                     /pseudo
     CDS             26143..26523
                     /locus_tag="VQ514_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809823.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(26703..27128)
                     /locus_tag="VQ514_000413"
     CDS             complement(26703..27128)
                     /locus_tag="VQ514_000413"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2499228.1"
                     /translation="MNNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKSLTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTCLTE"
     gene            complement(27196..27846)
                     /gene="phoU"
                     /locus_tag="VQ514_000414"
     CDS             complement(27196..27846)
                     /gene="phoU"
                     /locus_tag="VQ514_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET2499229.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(27861..28613)
                     /gene="pstB"
                     /locus_tag="VQ514_000415"
     CDS             complement(27861..28613)
                     /gene="pstB"
                     /locus_tag="VQ514_000415"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET2499230.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDVLLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(28615..29430)
                     /gene="pstA"
                     /locus_tag="VQ514_000416"
     CDS             complement(28615..29430)
                     /gene="pstA"
                     /locus_tag="VQ514_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET2499231.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(29423..30286)
                     /gene="pstC"
                     /locus_tag="VQ514_000417"
     CDS             complement(29423..30286)
                     /gene="pstC"
                     /locus_tag="VQ514_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET2499232.1"
                     /translation="MTKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRTFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(30404..31279)
                     /locus_tag="VQ514_000418"
     CDS             complement(30404..31279)
                     /locus_tag="VQ514_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HET2499233.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(31446..32777)
                     /locus_tag="VQ514_000419"
     CDS             complement(31446..32777)
                     /locus_tag="VQ514_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HET2499234.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGQGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSALKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(32778..33485)
                     /locus_tag="VQ514_000420"
     CDS             complement(32778..33485)
                     /locus_tag="VQ514_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2499235.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(33569..34030)
                     /locus_tag="VQ514_000421"
     CDS             complement(33569..34030)
                     /locus_tag="VQ514_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET2499236.1"
                     /translation="MIRTLELAPHVEGGYFRQTKKASDTINLAGKGRALYTSIYFLLE
                     ETNPSHFHRLTADEIWYFHAGSPLTVHMITADGHYEAVTLGLDISKGQQLHYCVPKGT
                     IWGSTVDKDYALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTRY"
BASE COUNT        10020 a         6414 c         7301 g        10303 t
ORIGIN      
        1 tcctagtttg cactttgatt ttcattgagt ataaaaagat cttgggaaaa gactcaattt
       61 cagtagaaaa ggaagtaaat cttcccacat taaaacgcat agtatcaagt tttttcaata
      121 cctgatagta tgcgtttttg cttttaacga ctttttgccc agtctcttat ttttcgataa
      181 ttttgtgggc gatcaattga cggtaacaaa tttgtttatt gtttttatta tcattaataa
      241 tctggagaat agtttcaagg gaaacacgac caagctctaa actattgata tcgacatagg
      301 ctgccaagtt gagcttggga ttaaccgagt caaagctgag aacagggaca tccagctggt
      361 gtttggcaat atagttacaa acgccttcag ctaggaggct atcggttgtg atgatagcat
      421 caatttgtgg atcgtgcttg aataatcgct tgctaaattt atagcccttt tcttccagaa
      481 actcgtcggc aaagtagatg cgattgttgt cagtggtaag tttgtaatgt ttaagcgcct
      541 gttcatagcc tgttaaacgg tctttggtca cgaagagctt tttacttcct ccgataaagg
      601 caatgcgttt gcagcctttt ttgatgaaat attcagtcgc atcaaaacca gcttgaacat
      661 tgtcgttgtc gacaagtggg atgaaaggag atagagattt acctaagata aggaagggga
      721 actgttcttc tgcgacgagt tttacgagag ggtcttcttc ttgggcatag agaaagatta
      781 gcccatctac acgcttgccg tagaccattt gtgaaatagc gttgagacgc tccttctcat
      841 ctttccctgt tgctatctga atggcatagt ggttttcaga tgcgacttga gagatgccac
      901 gtagaaccga tggaaagaaa ggattctggt agaaggcgtc tgagtcatca ggaagaacta
      961 atccgataac ctgagtatag ctgcttacca agctacgagc gttgaggttt gggtggtagt
     1021 tgagttcctt catagctttg cgaacgcgtt tttttgtttc gtcgctaatg gttgatttat
     1081 tttgaataac acgggttacg gttgaaggcg aaacaccagc agccttggcc acgtctttaa
     1141 tcgtaacggg cataaaaatc tcctatttat ggtaatttgg atcacggaaa tgtgttttat
     1201 aaaagatagt gaccagatac agaacaaaaa gaatattttg tacagtaatc agggttgtca
     1261 tattttggcc aaataatccc aaaataagtg taatcagagt cggcaatcct aaacagttca
     1321 agataaagtg atagcactct ttaaaggttc taaatgaaaa gaggcgtgat ttcttagtga
     1381 tataaaggag aagactagct cctagagaga cgataaagaa attcaaacca aagaggaagc
     1441 tcgcaccgag aactaggaag aggctgatat agatacgatt ttgttggtac cagtctttag
     1501 aaattgcttg ggtcaagctg tctttgcttt tgaaactctc agtctgaatg gctcggtaag
     1561 agatgcgggt cagttccttg ctttccttgc tgatgactag ttcatttgta tcgaaatgca
     1621 gttgcaagtc cttaggtaat tccttgattt gacttggacc aatcacaaca gaaggggctt
     1681 gactagctgt tccagtataa gttaatgtgc catcgacaat tgtagcatgt tcagagagat
     1741 cctggacaac ctcatttgtc agaggttcat agacattatc gataaaagtt tctagcggat
     1801 aggtctcctg ggagctgttt tggatagcaa tgggtaccat agacaagctg ataaggaaga
     1861 tactggtaaa gagaagttga aaccagttga gctcgaaacg tttggacagg ggcttacgaa
     1921 acccccaaat acttgaaaaa taggaaaatg gatatggaag cataggtatc tttctaaaag
     1981 gtgttttcta tatgagtatt attatataga gaaatgcgct ttatttcaag tatagggggt
     2041 aaattgcttg ctacaaggat attatttaca gtgacaattg ctaataatca atgaaaatca
     2101 aagtgcaaac tagccacagg ttgctcaaag cacaactttg aggttggaga taaagctgac
     2161 gtggtttgaa gagattttcg aagagtataa aatgaaaaag ggtggcgggg ataaattatc
     2221 ccttgtcgcc accacttgta agtcctgaaa caaagttctt ttgtaggaag aagaagagaa
     2281 tacagattgg aagggcgatg aggatagcac ctgctgagaa gtaggcaatc ttcaagtttt
     2341 tcgcattgtt aacgaaggtt tggagaccta cggcaacagt aaagtattct ttctcacgaa
     2401 gcaagaaact agagaggatg tagtccccga aaggtcccat gaaggcccag agagcttgta
     2461 cggcaaccat tgggcgaaca agtggtagaa caatttgcca gaagcggcgg aagtgtcctg
     2521 caccgtctag ttttgcagat tcgtctaaag acattggcac tgtatcgaag tagcctttca
     2581 tgagccaagc attcatcggg ataccaccac caacgtagag gaagatgagg aaccagttgt
     2641 ggttaagggc gttcaacata agcgccataa cgaagaaggc tgtcaaagcg gccatagttg
     2701 gcaccatttg gatgatcaag aagaagacca aactttgttt acgagccaag aagttgtaac
     2761 ggctgtaggc ataaccagca agtacgatga tacttgtttg aacagccatg gtaattaagg
     2821 cgataatcaa agtgttgagg taccaagtac cgtacaaggt ttcagtgaag aggcctttaa
     2881 agttatcaaa attgaggtcg atattagtat ctagtttaaa ggctgagacg ttacctgctt
     2941 taaaggctga cataatggta atcaacagtg gatagataat tacaattgat agaccaatca
     3001 ggtaaaggta agtaaggctt tgagtcagtc tacgtttgag tttaattgag ttattcatct
     3061 tagacgtcct ccatatcaaa tgcgtgtagt ttcttgaatg cgatcataga gattgagatg
     3121 acaatgatag agataatcaa ggtaacagct gccgccattg agtattgagg agatgtacct
     3181 gttgtcaaac ggtagatcca tgagatcaag atatcggttg aaccagctcc acctccgaca
     3241 ctaccaggtc ctccaccatt gaagaggtac atgatagaga agttgttaaa gttgaaggtg
     3301 tattggctaa tcaaagtagg tgccgcaaca gccaaaatca ttgggaaagt gatgttgcgg
     3361 aatttttgcc aagcgttggc accgtcaata taagctgctt cgtaaaggtc gttaggaata
     3421 gattgcaaga tacccaaagt caaaacgtag atgtatggga atccgagcca accttgcatc
     3481 ataatcaagg caatcttagt ccaagttggg tctgttttcc aaggaataag agctccatca
     3541 aggaaaggaa ggaatttagc caagattggc aatacttgag tgttgatagc accgacacta
     3601 tcgttaaaca tgtttgagaa tgtcaagata gtgatgaagg ctgggacagc ccaaggaaga
     3661 aggaaaataa caccaaagat acgttttcct ttgataaatg gttggttggc aatgatagct
     3721 gtgaagatac caattacgat ttgtaaagta gaagctgcca aagcccaaat gatagtccaa
     3781 gaaagaacag aaccaaaggc agaacggaag gtactcaagc tccaaatgtt tgtaaagttg
     3841 gtcaaaccaa cccagtccaa caacttgttt ggtggcaagt gttggaagtc gtagttggta
     3901 aaggcgatca tcaaggttac gataactggg aagataatcg cgaaggtcat ggcaacataa
     3961 gatggaatga tcaagaggta agggaagcca ttttcataaa tccctttgat catgtctttg
     4021 agtgtgcgtg gaactggaat tccattgtta atgcgttttg caatcgtatg tgcatctttg
     4081 atatttgaga aataaaagag tacataaacg attacaaaga ttagatggaa ggcaccacga
     4141 atcagcataa agagggaatt atcacgacct ggtttgtcac caagagtgat gaggttgctc
     4201 aattcagggg ttgcaagtgc taggaagtaa aggacaaata cgatggttac accaaggaag
     4261 ataaaacctt tggctttttg tttattgtaa atctgtccta acccaggaat gatagacagc
     4321 agggctgctt tactaggttg ttgcttttcc atattaactc ctttcatagg atactacttt
     4381 tttataagaa agctaaatta tttgtgtctc tattgataaa ttcaaagggg gatttgattt
     4441 ccacccccct tgaacaaatt ttttattcac caaatttttg tttgattgtt tctttgatca
     4501 atgttacagc atcgttagca gctgttttag catctttttg accacttaca gcatcaaaga
     4561 gcatattttt cgctggatcc caaactgcag acatttgaga gatgtttggc agtggttgag
     4621 tgttcttgaa ctgtttgata acagctgttg tcaactcatc gtttttacct tcagcgtatg
     4681 aacgagcctc agtattagct gggatttcgt tagtcttatc atataatact ttttgttgtt
     4741 cagttgcaac aaggaagtct acaaattttt gagaagcttc aaggttctta acggcttgag
     4801 gaatgaccca agctttacca ccaccgaatg cagcatattc ttttccattt ggaagagttg
     4861 ggatagttgc aactccgtag tttactttag catctttaaa ggcttgagct ttccaaggtc
     4921 cgtcgatgat agcagctgtt ttaccttctt ggaattgagt ttggattaag tttccagcac
     4981 cttctgtatc ttgcatacct ttaggccatt tttcgtacca agatttagcg tagttgatac
     5041 ctgcgataga accgtcgttt gcaagaccga tgtctttagc gtctttaccg ttttggccaa
     5101 agacgtaagc accgttaccg gcaagaagtc catatgtata gtagaagttt gtccagtcag
     5161 ctaggaaggc agtagtttta ccatcttcac cagcgaatgc gtatttgcta tctttagcaa
     5221 ggttttccaa gtcagcaaat gtttttggag catctttcac caagtctttg ttgtagtaca
     5281 taacaagtga ctcgataacg gcaggagcac cgtaaacttt accattagca gctgttacaa
     5341 gagatttagt tgtgtcgtct gttttagcac catcgctcaa tttcacttct gaaagttgtc
     5401 cgtcagaacc aaggctacct acacggtcgt atggagccat cataacatca gggacattac
     5461 cagattggtt gtcaagagaa agtttatcaa gacctcctag agcatcacca gttttaagag
     5521 tgacttttac tccagcttct ttttcataag ctttagcaac ctcttcaata tagctcttat
     5581 atccctcgtc tacatataca gtgagttctt tgacttcaga tgaaccagaa tcagcaggct
     5641 tatcagcagt tttgcttccg caagctacca aaagcaagct agcaagtgta gcagttccaa
     5701 gcaccgcagt gctcttcata aatttagatg acatagtgta ttcctcccaa agaatagcaa
     5761 gttttattga taaggaaacg caaacgtttt cctttatgag cttagtatag cacaaataga
     5821 aaacggttgc aagtattttt tgtaaaaatt ttaaaaaatt tttaagcttg atttgatagc
     5881 ataattttgc attttaatag aagaaaagta tgaaatagat aagaatacag tatttttaat
     5941 aaaaatatat ggaatctgtc taaagaggga aatcaggagg ggctacctcc ctgtttctaa
     6001 tccctatcta ttttctatag acgtttgtgc tttgttgcat ataaattcaa gctccctaag
     6061 gaggttttgt actttgtaga ctgtctcgtg agagtcaaac tttaggatta gatagtttta
     6121 agttatcttt gctaaaatga gttagtctcc ttaaaaataa tcaaaaaagt tttttaaaaa
     6181 cgcttgcaat tatgcttgaa aaggagtata cttataagta acgcaaacgt ttgcgtctgc
     6241 aaaatacgca acgttccatt attttaacac acgaggtgct attatgaaaa aacgtcaaag
     6301 tggtgtgttg atgcacatct cttctcttcc aggagcttac ggaatcggat catttggtca
     6361 aagtgcttac gacttcgttg atttcttggt ccgtacaaaa caacgttact ggcaaatcct
     6421 tccattagga gcaactagtt acggggattc tccttaccaa tctttctcag ccttcgcagg
     6481 aaacactcat tttatcgatt tagatatctt ggtggagcaa ggtttgttgg gagcgagtga
     6541 ccttgaagga gttgactttg gtagcgatgc gtctgaagtt gactatgcta aaatctacta
     6601 tgcacgtcgt cctcttttag aaaaagcggt gaaacgtttc tttgaagtcg gagatgttaa
     6661 agattttgag aaatttgctc aagacaacca atcatggctt gagctctttg ctgagtatat
     6721 ggctatcaaa gagcattttg acaatcttgc ttggactgaa tggccagatg cagatgctcg
     6781 tgctcgtaaa gcttcagcac ttgaaagcta tcgtgagcaa ttggcagaca agttggttta
     6841 ccaccgtgtg actcaatact tcttcttcca acaatggttg aaattgaaag cttacgctaa
     6901 cgacaaccac attgaaatcg ttggggacat gccaatctac gtagcggaag attcaagtga
     6961 tatgtgggca aatccacatc tcttcaaaac agatgtcaat ggtaaggcta cttgtatcgc
     7021 aggatgccca ccagatgagt tttctgtaac tggtcagctt tggggtaacc caatctatga
     7081 ctgggaagca atggacaaag acggctacaa atggtggatt gaacgcttgc gtgaaagctt
     7141 caaaatctac gatatcgttc gtatcgacca cttccgtggc ttcgaatctt attgggaaat
     7201 ccctgctggt tccgatacag cagcacctgg tgagtgggtg aaaggtccag gctacaagct
     7261 ttttgcagcc gttaaggaag aacttggtga gctaaacatc atcgcagaag accttggctt
     7321 catgacagat gaagtgatcg aattacgtga acgtactggc ttcccaggaa tgaagattct
     7381 tcaatttgcc ttcaacccag aagacgaaag cattgatagc ccacacttgg cacctgctaa
     7441 ctcagttatg tacacaggaa cacacgataa caatacggtt cttggttggt accgtaatga
     7501 gattgatgat gcgactcgtg agtacatggc tcgttacacg aaccgtaaag aatacgaaac
     7561 agtggtacac gctatgcttc gtacagtatt ttcatcagtt agctttatgg caattgcaac
     7621 tatgcaagat ttactagaat tggatgaggc agctcgtatg aacttcccat ctacccttgg
     7681 tggaaactgg tcttggcgta tgactgaaga tcaattgaca ccagctgtcg aggaaggttt
     7741 gcttgacttg acaacaattt atcgccgaat taatgaaaat ttggtagatt taaagaaata
     7801 agacaataat caggagacaa ctaaacatgt tatcactaca agaatttgta caaaatcgtt
     7861 acaataaaac cattgcagaa tgtagcaatg aagagcttta ccttgctctt cttaactaca
     7921 gcaagcttgc aagcagccaa aaaccagtca acactggtaa gaaaaaagtt tactacatct
     7981 cagctgagtt cttgattggt aaactcttgt caaacaactt gattaacctt ggtctttacg
     8041 acgatgttaa aaaagaactt gcagctgcag gtaaagactt gatcgaagtt gaagaagttg
     8101 aattggaacc atctcttggt aatggtggtt tgggacgttt ggctgcctgc tttatcgact
     8161 caattgctac tcttggtttg aatggtgacg gtgttggtct taactaccac tttggtcttt
     8221 tccaacaagt tcttaaaaac aaccaacaag aaacaattcc aaatgcatgg ttgacagagc
     8281 aaaactggtt ggttcgctca agccgtagct accaagtacc atttgcagac tttactttga
     8341 aatcaactct ttacgatatt gatgttactg gttatgaaac agcgactaaa aaccgcttgc
     8401 gtttgtttga cttggattca gttgattctt ctattattaa agatggtatc aactttgaca
     8461 agacagatat cgctcgcaac ttgactctct tcctttaccc agatgatagt gaccgtcaag
     8521 gtgaattgct ccgtatcttc caacaatact tcatggtttc aaacggtgcg caattgatca
     8581 tcgacgaagc aatcgaaaaa ggaagcaact tgcatgacct tgctgactac gcagttgtcc
     8641 aaatcaacga tactcaccca tcaatggtga ttcctgaatt gattcgtctt ttgactgcac
     8701 gtggtatcga gcttgacgaa gcaatctcaa ttgttcgtag catgactgcc tacactaacc
     8761 acacaatcct tgctgaggcg cttgaaaaat ggcctcttga attcttgcaa gaagtggttc
     8821 ctcacttggt accaatcatc gaagaattgg accgtcgtgt gaaggcagag tacaaagatc
     8881 cagctgttca aatcatcgat gagagcggac gtgttcacat ggctcacatg gatatccact
     8941 acggatacag tgttaacggg gttgcagcac ttcatactga aatcttgaaa aattctgagt
     9001 tgaaagcctt ctacgacctt tacccagaaa agttcaacaa caaaacaaac ggtatcactt
     9061 tccgtcgttg gcttatgcat gctaacccaa gattgtctca ctacttggat gagattcttg
     9121 gagatggttg gcaccatgaa gcagatgagc ttgaaaaact gttgtcttat gaagacaaag
     9181 cagctgtcaa agaaaaattg gaaagcatca aggctcacaa caaacgtaaa ttggctcgtc
     9241 acttgaaaga acaccaaggt gtggaaatca atccaaattc tatctttgat atccaaatca
     9301 aacgtcttca cgagtacaaa cgccaacaaa tgaacgcttt gtacgtgatc cacaaatacc
     9361 ttgacatcaa agctggtaac atccctgctc gtccaatcac aatcttcttt ggtggtaaag
     9421 cagctccagc ctacacaatc gctcaagaca ttatccattt aatcctttgc atgtcagaag
     9481 ttattgctaa cgatccagca gtagctccac acttgcaagt agttatggtt gaaaactaca
     9541 acgttactgc agcaagtttc cttatcccag catgtgatat ctcagaacaa atctcacttg
     9601 cttctaaaga agcttcaggt actggtaaca tgaaattcat gttgaacgga gctttgacac
     9661 ttggtactat ggacggtgct aacgtggaaa tcgctgagtt ggttggagaa gaaaacatct
     9721 acatcttcgg tgaagattca gaaactgtta tcgaccttta cgcaaaagca gcttacaaat
     9781 caagcgaatt ctacgctcgt gaagctatca aaccattggt tgacttcatc gttagtgatg
     9841 cagttcttgc agctggaaac aaagagcgct tggaacgtct ttacaatgaa ttgatcaaca
     9901 aagactggtt catgactctt cttgatttgg aagactacat caaagtcaaa gagcaaatgc
     9961 ttgctgacta cgaagaccgt gacgcatggt tggataaagt catcgttaac atttctaaag
    10021 caggattctt ctcatctgac cgtacaatcg ctcagtataa cgaagacatc tggcacttga
    10081 actaatactc ttcgaaaatc tcttcaaacc acgtcagctt tatctgcaac ctcaaagcgg
    10141 tgctttgagc aacctgcggc tagcttccta gtttgctctt tgattttcat tgagtataag
    10201 atacaaattt atactaatac atcttgtaaa aaagcgagtt tcgattgaaa ttcgcttttt
    10261 taaatgctgt ggatttgggt caatcttgtc taaaaatagg gaaatcctag atacagtgaa
    10321 ggctttaaat gctggttttt actgtcttca gccttatatt ttttcgtagt tggttacctc
    10381 atatctcttg tattcgctta cataaagtat tataatataa ttgtaggaaa gaaggtgttt
    10441 ttatgatata cacacttaaa ttggtgttgt ttattacctt tcttgtaata agcttgttac
    10501 ctgataagat ttttggaaaa aataaaaaaa tttggaaaat agtttttgca atattgacgg
    10561 cagtggcagc attgtcattt atgtactaag ttattttaag aatgtaggga aataaaccct
    10621 acattctttt tagttttttc tgttttctaa attctattta tccaagcgct tcaacatttc
    10681 ttgcttcttc gcttcaagtt ctgcacgctt ttcttcgatt tcggcatgtt ttttctcgag
    10741 ttcagaacaa cttgcatcat tgctaaattc ttttcgccat caggagatag ggtgagtcga
    10801 catgtctatt actcacccaa agcagtccta caaagcagga attttctgtt acttttttgg
    10861 aaatagtaac gtttatacag ctttgacact tcgtatcaaa gcgccaaaca cactccgagg
    10921 gatttacaga aagcagaaaa ggaatgatct ggtataagat cattcctttt ctctcttttt
    10981 ctttaagtaa ttatatacaa tgtacgacga agtcgtcatt gcaatgctga tccaccacct
    11041 aaagggaact ttaaacaaca ttgataagat aaagaatata aacaacgaaa atacgttata
    11101 cccaattaat tttattgtat atctcatgat taaaagttaa tccttccgtt gttaggaatg
    11161 gcatcatttt tatcccataa ttgtgctaaa taagtccccg gtgataataa attcatagcg
    11221 aattctaaag caacatcatt tacaaaccaa ctacctagat atctagaaat tgctgaacga
    11281 atagcacttt ttgctacatg ttttcctttt actttaatta gatttgcaag gcctgcagta
    11341 gttcctccta atgctaaagc tattgcagta tctaatagag cacccatttg attaactgta
    11401 ataccttgcc aagctgctct aaatggagag tatgtaggtg ggattgtata atcgccttgt
    11461 aattgtcggt taattacttc tttgatccat tgttgtgaga cgtctggatg aaaagattgg
    11521 atttcgtttg caagtgtatt gatttgttct tctgttagag aagtgacagg ttgaagttcc
    11581 atatttgttt caatttgtga tacttgttca gaagcgtata cagctgaaac acttggaatc
    11641 gctgatacaa ttaacacaat tgacgtcaaa aaaaccgaaa taaatttcat taatttgtcc
    11701 atgagctttt ctccttttta tttgtatctg cttacatttt atcatatact gttattatag
    11761 tcaaaaaaat atgctattat gttaaaaaaa tatttttcaa aatataaatg gacggattta
    11821 ttttggattt tatttgttat tttgacctgc ctctatattg gtaaccatga tttgtttact
    11881 ctcaatcatc aagaattctc ttttcgtggt agcgtttggg gtctggtact ggccttatat
    11941 cacttattgt tcattgataa gtttgtaatt tcgaatcgaa aataaagatt agaactatga
    12001 ttgactggct acttttaata ttgattttgg aggaagattt tgtctctatt atttattatt
    12061 ttaaatttat ttattttgta taagatctat tctttgaggc gggaaaaatc gaaatacttg
    12121 atttatacgg cctatatcat atttggggta aatgtagtat atggtattca atggttatta
    12181 agagaactga tttcaactat ttccccttaa tgtgtatgga aactataaca aaacgcataa
    12241 gattaaggtt tgtgtacctg ttcttatgcg tttttgtcat attgaagatt ttcggtaagt
    12301 tttatacctt tgggaagtct agtttagaat gctttcccaa ccaaaatctc tgcttcgatg
    12361 gtaatctctg tcagttggct ccagtcaatc ttgctctggt caacgtgaaa gaggagagga
    12421 gtcatactga gtagggcttg gagttgttct gctgtgatag tcttagtcag agaggcagtt
    12481 tgactagata ggatggtaaa gtgttcctgg aaatgctcct tgatatcttg gttagaatac
    12541 tccttgtttg tcagctggtc ctgtactctt tgacggattt ctttgaggtg attttcagtt
    12601 gggataacct ttatcaagat accgtctttg gataaaacgc gacgaaattc tccatagttg
    12661 gcaggtgaga agatatcaag cagaatatcc atgttagcgt cttttatagg aagtcgtgcc
    12721 aagtcgccaa caaaccaatt gactgcccag ttgggttcac ttttagcagc gatttggact
    12781 gaatctttgg agatgtcaaa ggcatagaaa gttttttcag agtgactttc ttgtagtttg
    12841 cgagaataga atccttcacc acaaccgata tccaaaattg ttgtggtagt ttttgagctt
    12901 gcaagcaagt cagatacagc atctaagata gcttggtaaa agccggcttc taggatttgt
    12961 tgacggtttt gaaaattttc cttgttatag ttagcagatt gcttgatttg aggtgctaga
    13021 ttgacatagc caaatttcgc caagtcaaaa gaatgacggt tgcagcactt gaaattagtc
    13081 tctaacagag tcagattttc ttgacagata ggacaggcaa aggcagtcgc agaagcaaaa
    13141 cgctgaagtt tgggcttgag atttgtattc atagtttaag tgtatcaaaa gaggcaaatg
    13201 aaagcaactt taggaataag tagatgggag attcagtaga aataaaacta attctccaat
    13261 ttatagaatg aaattgcttt tatatctaaa ttgctatata ataatgataa ggaggaaata
    13321 agtatgttaa aagaagtatt aaccgttgca aaagttgcga aaaaatcttc attatttttg
    13381 ggtggtgtcg catttggtac ccttggtttg aaaatcttag caagtaagga agctaaaaaa
    13441 ggttattcta aagctttggc taaggcttac aagttgaaag acgagctaga tgcatctgtt
    13501 tctgttgtga agcaacatgg agacgatgtc ttgcaagatg ccaaatattt gtacgagcaa
    13561 gagaaaaaag aagagcaatt agatagcctt ataggtgaat aatatgtctt ttaaagtgct
    13621 acatagagga taccaacata tccgactatc atcttctttt tcacttacct tggatattca
    13681 agactatctt cgttccttgg cgagagatga aaaggggatt gagtttatcc agttttacat
    13741 ggatcaacag cactttactc tacgcataaa agaaggcttt tctgtattag ataatgcaga
    13801 agccttttta aaaagaattg ataaagggaa agtttctgag ttgatgactc ttcccattcg
    13861 tagagaagag agtgcttatt ctattgtttc aggtgcagcg gttaagcgtg tactttttcg
    13921 tagttttgtg ccgtatccta ttcgctatat atggacttgt tatcaggctt tgggttatat
    13981 tagagaagcc tatcaaacac tagcgcgtaa ggaactaacg atggaagtct tggactgttc
    14041 ggctatttta ttgtctttgt ttatgaacca atccaagaca gctagcaata tcatgtttat
    14101 gcttgatttg gggaatcatt tagatcagtg gtccttgaaa aaaactgcaa cagatttaga
    14161 acagagtctt cttgcaaaag agagcgatgt attcctagta cagggcgata cggttgttag
    14221 tatcaagagt tccgatgttc aaataggaga tgtcttgatc ttatctcaag gaaatgaaat
    14281 tctgtttgat ggacaagtag tttcaggttt aggtatggtc aacgaaagtt ccttgacagg
    14341 agagagtttt ccagttgaaa aaagagagtc tgatttggtt tgtgcaaata cagtattaga
    14401 aactggagag ttacgcattc gtgtaacaga taatcagatg aacagccgta ttttacagct
    14461 gattaagttg atgaagaaat ctgaagaaaa caagaaaacg aaacaacgct atttcatcaa
    14521 gatggcggat aaagtcgtca aatataattt ctttggggct gggctgactt acctattgac
    14581 aggttctttt tctaaagcta tttctttcct attggtcgat ttctcctgcg ctttgaaaat
    14641 ctctactcct gtagcttatt tgacagttat caaggaaggg ttgaaccgtg aaatggtgat
    14701 taaggatgga gatgttctgg agaaatatct ggaagttgat actttcttgt ttgataagac
    14761 aggaacaatc acaactagtt atcctatagt tgaaaaggtg ttaccttttg gagactatag
    14821 tgaggaagat attctcagaa tcagtgcctg tcttgaggaa cacatttatc atcctattgc
    14881 taatgccatc gtcaagcaag ctgagataga gggaattgaa catgaggaaa tgcatgggaa
    14941 actccaatat atcgcaagca aggggatcaa atctcatata gatggccaac cagttcttat
    15001 tgggaattat gtcttgatgc aggatgagca gattcatatc agttcagaac aaaatgcttt
    15061 aattgaagag tacaagagtc actacaatct cttattcttg gcttatcaaa atgaattgat
    15121 tggaatgttc tgcattcata ctcctttgag aaaagaagca aaaacagcct tggataaact
    15181 taaggcacag gggaaaaaat tgattctggc aacaggagac accttgatta ggacagagga
    15241 attagtcaaa gatttgccct ttgatcaggt ctatacagac ttgaaacctg atgggaaatt
    15301 tgagttagta gagaaactgc agaaagcagg tcacactatt ttgatggttg gagatggatt
    15361 gaatgactca gcggctttaa ctctatcaga tatcggtgtg gtgatgaatg agagtgcaga
    15421 tatttctaag cagatgagtg atatcttatt gttagataat cgtttggatt tcttccaaga
    15481 gttggattcg ttatcatcat ctttgcaaac actcatcaag aaaaatattc aggataccgt
    15541 tgtcgtaaat agtagtttga ttggctttgg cttatttaat tggttcagtc cttcaaatct
    15601 ctctatctta cataatctaa caaccttgcg cattgtcctg cgtagcctgt ctattaaaaa
    15661 tagatagatg agagttatta acagcaaaaa ggagttacct ttctcgaggt taactccttt
    15721 gtttatagat aaatgttgaa tagtttagta agtcaataca aagtattttt tcttcccacg
    15781 acggataaca gtcagttcat tttctaactt atcagcgtca ctcaagacat agtcaaggtc
    15841 ttggatgcgg tcgccgttta cgtagatggc tccgttttgg acgtcttcac gggcttggcg
    15901 ttttgagtta actataccag atgagacgag cagttccacg atattgttgt tttcgtctgc
    15961 ctgtacttgg tagtttggca caccacgaag tccttgtttg agctctttga cagaaaggtt
    16021 tttgatgttt cctgcaaaga gttgctcagt gatgttaagt gcttctttgt aggcttcttc
    16081 tccgtgaaca agtgtaacaa cttcacgagc caagactttt tgagccaagc gttcgtgtgg
    16141 cgctgcttca aattgtttac gaatatcttc aatctcatca agtgacaaga aagtaaagac
    16201 tttcaagaag cgaacagcgt cagcgtccat cacgttcatc cagaattggt acatttcgta
    16261 tggagaagtc ttttcgggat tgagccagac ggcatttcct tctgatttac caaatttctt
    16321 accagttgca tctgtgatta gtggaacagt gataacgtga ccagtcttgt ccgccttacg
    16381 acgaagcaat tcggtaccag ctgtcatatt tccccactgg tcagaaccac cgatttgaag
    16441 agtgacatta tggtcttggt taaggacgaa gaagtcgtac ccttgcatga tttggtaagc
    16501 gaactcagtg taagaaattc ctgtttcgat ccgtttttta acagattcct tactcatcat
    16561 gtagttgacc gtgaagtatt ttccaatatc acggaggaag tcaatgaagc tgatgctgcc
    16621 aaaccagtcg tagttgttga ccatgacagc cttgttttcg ccattttcaa agtcaagaaa
    16681 acgagaaagt tgtccttgga tagacttgac ccagccatct actgtgtctt ttgtttggag
    16741 actacgttca gcatctttga aggacggatc tccgatgaga cctgtagcac cgccaacgag
    16801 cgcataaggt ttgtgacctg ctagttgcaa gcgacgactt gtcaagattg cgacaaggtg
    16861 gcctaggtga aggctgtcag cagttggatc gtagccagta taataagaaa cttgaccttc
    16921 ttctagggct ttacgcaaag cttcttcatc agtcgtttga aatatcaaac cacgctcttt
    16981 tagctcatca aaaatgtgca tgtgtctttt ctccttttta aaattattgt ttctacctat
    17041 tgtatcacaa acttgggcaa tagcctagca gaatagggaa gaaagtggct attttattga
    17101 aaatttccct tttgtggtat aatagataga gtgaggaaat ccatgcaaaa tcaattaaat
    17161 gaattaaaac gaaaaatgct ggaatttttc cagcaaaaac aaaaaaataa aaaatcagct
    17221 agacctggca agaaaggttc aagtaccaaa aaatctaaaa ccttagataa gtcagccatt
    17281 ttcccagcta ttttactgag tataaaagcc ttatttaact tactctttgt actcggtttt
    17341 ctaggaggaa tgttgggagc tgggattgct ttgggatacg gagtggcctt atttgacaag
    17401 gttcgggtgc ctcagacaga agaattggtg aatcaggtca aggacatctc ttctatttca
    17461 gagattacct attcggacgg gacggtgatt gcttccatag agagtgattt gttgcgcact
    17521 tctatctcat ctgagcaaat ttcggaaaat ctgaagaagg ctatcattgc gacagaagat
    17581 gaacacttta aagaacataa gggtgtagta cccaaggcgg tgattcgtgc gaccttgggg
    17641 aaatttgtag gtttgggttc ctctagtggg ggttcaacct tgacccagca actaattaaa
    17701 cagcaggtgg ttggggatgc gccgaccttg gctcgtaagg cggcagagat tgtggatgct
    17761 cttgccttgg aacgcgccat gaataaagat gagattttaa cgacctatct caatgtggct
    17821 ccctttggcc gaaataataa gggacagaat attgcagggg ctcggcaagc agctgaggga
    17881 attttcggtg tagatgccag tcagttgact gttcctcaag cagcattttt agcaggactt
    17941 ccacagagtc ccattactta ctctccttat gaaaatactg gggagttgaa gagtgatgaa
    18001 gacctagaaa ttggcttaag acgggctaag gcagttcttt acagtatgta tcgtacaggt
    18061 gcattaagca aagacgagta ttctcagtac aaggattatg accttaaaca ggacttttta
    18121 ccatcgggca cggttgcagg aatttcacga gactatttat actttacaac tttggcagaa
    18181 gctcaagaac gtatgtatga ttatctagct cagagagaca atgtctccgc taaggagttg
    18241 aaaaatgagg caactcagaa gttttatcga gatttggcag ccaaggaaat tgaaaatggt
    18301 ggttataaga ttactactac catagatcag aaaattcatt ctgccatgca aagtgcggtt
    18361 gctgattatg gctatctttt agacgatgga acaggtcgtg tagaagtagg gaatgtcttg
    18421 atggacaacc aaacaggtgc tattctaggc tttgtaggtg gtcgtaatta tcaagaaaat
    18481 caaaataatc atgcctttga taccaaacgt tcgccagctt ctactaccaa gcccttgctg
    18541 gcctacggta ttgctattga ccagggcttg atgggaagtg aaacgattct atctaactat
    18601 ccaacaaact ttgctaatgg caatccgatt atgtatgcta atagcaaggg aacaggaatg
    18661 atgaccttgg gagaagctct gaactattca tggaatatcc ctgcttactg gacctatcgt
    18721 atgctccgtg aaaagggtgt tgatgtcaag ggttatatgg aaaagatggg ttacgagatt
    18781 cctgagtacg gtattgagag cttgccaatg ggtggtggta ttgaagtcac agttgcccag
    18841 cataccaatg gctatcagac cttagctaat aatggagttt atcatcagaa gcatgtgatt
    18901 tcaaagattg aagcagcaga tggtagagtg gtgtatgagt atcaggataa accggttcaa
    18961 gtctattcaa aagctactgc gacgattatg cagggattgc tacgagaagt tctatcctct
    19021 cgtgtgacaa caaccttcaa gtctaacctg acttctttaa atcttactct ggctaatgca
    19081 gattggattg ggaagactgg tacaaccaac caagacgaaa atatgtggct catgctttcg
    19141 acacctagat taaccctagg tggctggatt gggcatgatg ataatcattc attgtcacgt
    19201 agagcaggtt attctaataa ctctaattac atggctcatc tggtaaatgc gattcagcaa
    19261 gcttccccaa gcatttgggg gaacgagcgc tttgctttag atcctagtgt agtgaaatcg
    19321 gaagtcttga aatcaacagg tcaaaaacca gggaaggttt ctgttgaagg aaaagaagta
    19381 gaggtcacag gttcgactgt taccagctat tgggctaata agtcaggagc gctagcgaca
    19441 agttatcgct ttgctattgg cggaagtgat gcggattatc agaatgcttg gtctagtatt
    19501 gtggggagtc taccaactcc atccagctcc agcagttcaa gtagtagttc tagcgatagc
    19561 agtaactcaa gtactacacg accttcttct tcaagggcga gacgataatt ctctaaatga
    19621 agtggccaat caatggattg ccactttttt cctttcgtgt tacaataagg gtatgaatca
    19681 gtatcagaaa aagattgtta acggaaaaat ttattcgctc ctatccggct taatatgggg
    19741 aatctgtgga attttaggag agtacttctt tactcattat caggtgtctt cgggctggat
    19801 tacctctatg cgtttgacac tggcagggag tcttgtactc atttggtctg caatacaatt
    19861 aaaatcgcaa gtgctagata tttggcgaga caagaaaaat tacctgccct ttttagctta
    19921 tgctattttg gggatttttt cagtccagta ttttttctat ctctgtgtag aatactcaaa
    19981 tgctacgaca gcaactattt tacagtttat tagccctgtc tttatcctct tttacaatcg
    20041 cttggtttat caaaaacgag cgtcaaaaag cgctgttttc tatgttttgg ttgccatgct
    20101 gggtgtttgc ttgatggcga caaagggaga tctctctcag ttatccatga cgccgctagc
    20161 tcttataaca ggtttgctga gtgccatggg tgttatgttt aatgttatct tgccccaacc
    20221 ttttgctaag cgttatggtt ttgttcctac ggttgggtgg gggatgattt tggcaggttt
    20281 gtttagcaat gtcctctcgc cggtttatca gctttccttt actcttgata tttggagtat
    20341 cttgatttgc ctcattatcg ctttctttgg aacggctttt gcttttttca tttccatgaa
    20401 ggctgtgtcc ttggtttctc ctttggtggt tgccgttatc agtgccagtg aacctctctc
    20461 ttctgctctc ttgagtgttt tgttcttagg attagtagtg gattggtccc tccttctagc
    20521 tatagccttg attattttac ccatgatatt tttgtctata gaagaagcga aagaaagtag
    20581 ataaaaagtc ttttcttaat tctaaaagtg tgctatacta gaatagtcaa taaaacacgg
    20641 ggagatagtt gtgaagagtg tttttaggtt gtatcggtag gggcttgctt ttaccgacag
    20701 cacgttttat ctcacatccc taaaaaatca tacctaaaaa cagacaaaaa ggtttcaaat
    20761 ttgaggcctt ttttggtaga ataggtatca ttataacgaa ccaggaggca cctatgactg
    20821 ctacaaaaat gaacgctcaa gaaattatcc aatttatcgc caatgctgaa aagaaaacca
    20881 gtgtcaaagt aacctttgag gggcaactcg caactgctgt gcctagctct gttgtcaaac
    20941 taggaaatgt cctattcgga gactggaagg acgtggctcc gcttcttgaa ggtttggtag
    21001 aaaatcaaga ttatgttgtc gagcaagatg ctcgtaattc tgcagttcct ttgctagata
    21061 agcgtgctat caacgctcgt atcgagccag gtgctattat ccgtgaccag gtggaaattg
    21121 gtgacaatgc tgttatcatg atgggagctg ttatcaatat cggtgctgaa atcggtgctg
    21181 gaaccatgat tgacatgggt gccatccttg gtggccgtgc tatcgttgga aaaaatagcc
    21241 acgttggtgc aggtgcagtt ttggcaggtg tgattgagcc agctagtgct gaaccagtct
    21301 gtgtcggaga caatgttctt atcggggcta atgcagtggt tatcgaagga gtccaaatcg
    21361 gtagtggttc agttgtcgct gcaggagcta tcgttaccca agatgtccca gaaaacgtgg
    21421 tagtagcagg tgttccagct cgtattatca aagaaattga tgcccaaact caacaaaaaa
    21481 cagcgctaga ggatgcgctt cgtaccttgt aattgtaaaa gtaaaaaaga ggcggaaccc
    21541 tttttccagc ctctttctgc tatatcggag gacagataga tgttagattt gattcagact
    21601 agacgagctt tacaccagat tccagagatt ggcttggagg agttcaagac tcaggcttat
    21661 ttgctggatg tgattgagaa attgactacg ggcaaggatt ttgttcaaat tcgtacttgg
    21721 cggacaggga ttttggtcta cctgcaggga agtcagccgg agcgaaccat tggttggcgg
    21781 acagatattg atggcctgcc tatcgtcgaa caaacaggtc taccttttac ttctcaacac
    21841 caaggtcgca tgcatgcctg tggtcatgat tttcatatga ccattgcctt aggctgtctt
    21901 gagcgcgccc ttgaggagca acccaagaat aatctgctct ttctatttca gcctgctgaa
    21961 gaaaatgaag ctggtggtat gctcatgtat gaggacggtg cttttggaga ttggttgcca
    22021 aaccaattct atggtctcca tgttcgtcca gatttgaaag tcggccagat tgcgactaat
    22081 actcatacac tctttgcagg aacttgcgag gtgaagatcc gtttcaaagg taaaggtggc
    22141 cacgcagctt ttcctcatga agccaatgac gccttggtgg cggctagtta ctttgtgacc
    22201 caagtgcagt cagttgttag ccgcaatgtc aacccaatcg agggagcagt ggtgaccttc
    22261 ggcgtttttc aagctggaac caccaacaat gtcatcacag acacagcttt tttacatgga
    22321 actattcgtg ccttgacaca ggacatgagt ctcttggtgc aaaagagggt aaaaacagtt
    22381 gcagaagggg ttgcagctgc ctttgatatg gaagtcgaag tggaactcaa acaaggtgga
    22441 tacctacctg ttgagaacaa tccagccttg gcgcgtgaac tgatggactt ctttgacgag
    22501 aaagacggaa tcgagttgat tgatatcgaa cctgctatga caggcgagga ctttggttat
    22561 ctcctttcaa aggttgatgg cgtgatgttt tggctaggta tcgatagtcc ctacgctctt
    22621 catcaccctc aaatgagtcc taaggaagaa gtcttagcta ttggggtggc tgcggtgtct
    22681 agtttcctga aaaagaaggc agcagaatag agggcttgtc tatgaaatcg gaattacgca
    22741 agcaagtctt gcatgaaatg aaggctatat ctcaagagca aaaacaggct atagaccaag
    22801 ctttaaccga gcgaatttta caacacccct tttatcaaga agccaaggtc atcgcaacct
    22861 acctctcttt ttctcatgag tttcaaacgc gggaactgat tgagcaggcg ctgaaggacg
    22921 gcaagaaggt tttgacacct aagacctatc ccaaggggcg catggatttt gtggtctatg
    22981 atccgcaaca gttggtaaaa acttcctttg ccttactgga gccacaggga gatttggaag
    23041 tagtggatgc atctcagatt gatttgattc atgttcctgg cctggctttt acgacggatg
    23101 gatatcggat tggatatggt ggaggttatt atgaccgcta tctggaacat ttttctggtc
    23161 gtgctttgag tacgatatat ccttgtcaaa ttcaggactt tatacctgaa aaccatgata
    23221 ttcctgttca ggaggtatta attgatgaag gaaatctttg atagacgtta ccctgtgacg
    23281 agtttcttcc tcttagtgac ggccttggta tttttactaa tgttggtcac tgcaggcgga
    23341 aactttgaca gggcagatac attatttcga tttggagcca tgtatgggcc agctattcgc
    23401 ctctttcccg agcaggtttg gcgtctcttg tctgccattt ttgttcatat tgggtgggaa
    23461 catttcattg ttaatatgct ttcactttat tatcttggaa ggcaggtaga ggagattttc
    23521 ggttctaagc agtttttctt tctctatctt ttatcaggaa tgatgggcaa tctctttgtt
    23581 tttgtattta gtcctaaatc cttagcagca ggcgcctcta cctctcttta tgggctattt
    23641 gccgcgatta ttgttcttcg ctatgcaact cgcaatcctt atattcaaca gctagggcaa
    23701 tcctatctga cactttttgt agttaacatt attggaagtg ttctgattcc aggaatcagc
    23761 ctagcaggcc atatcggtgg tgcagttggt ggcgcatttc tagcagttat ctttccagtt
    23821 agaggagaaa aacggatgta taataccagc cagagattag gagcggtagt cttgttcgta
    23881 ggactcgcca ttttgctttt ctacaaggga atgggaatgt gacgaagttg aaagaaaatt
    23941 tgtgtaatga aaaaagataa ggcatttttt gaaccttatc tttttatttt attttattcc
    24001 ttctcagtca attcttttcc aagttggatg aggtaattct tcaaatcatc tttgacttgt
    24061 gggtgtttga gggcgtagtc gatggatgtt ttcatgaagc caaacttgtc tccgacatcg
    24121 taacgagccc cattgaactc acgagcaaat acacgttgtg ttttattgag ggtgtcgatt
    24181 gcatctgtca gctgaatttc atttcctgca cctggagctt ggttttcgag gatttggaaa
    24241 atttcaggcg tgaggaggta gcgtccgata atagcaaggt cgctaggagc gtcctctgga
    24301 gctggttttt caacaaaggt ttcaacactg taaagaccat cttttccttc gccttgcgga
    24361 gcaataaccc cgtaagcaga tacttcgtca tgagggactg gcatgacagc gatagtagac
    24421 gcgtgggtac gctcgtagtc atccatgagt tgtttggtaa gtggaacagc cttttcgtct
    24481 gtgatatcca tcaagtcatc accaagcata acgacaaaag gttcatttcc gacgaaagcc
    24541 ttagcttgca aaacagcatc tccgagaccg cgtggatgag tttggcggat aaaatgcaga
    24601 cgcatgtcag ttgttttatc aactagcttc aaaagatctg ttttcccttt ttctttgagg
    24661 ttatattcca attcgaagtt tgaatcaaag tggtcctcaa tagaacgttt tgatttacca
    24721 gtgacaacta gaatatcttc aatacctgat ttgagagcct cttccacgat aaactggata
    24781 gttggtttgt ctacgattgg caacatttct ttggcaaggg ccttggttgc tggtaaaaat
    24841 cgagttccta gtccagcagc agggatgact gcctttctaa cttttgatgt cataaaaatc
    24901 ctttctatag agggttaaga ccactcattt tctgctttaa attcattgtt catgatgtca
    24961 taaatggcat ctttgatatt ggttccgtga taaataactt ggtaaatagc ctgtgtaatg
    25021 ggcatataga ctccaagttc ttgggctagt tcataggctg ctcgagtcgt tgaaattcct
    25081 tcgattacca tgcccatatt agcttctata tcagctaggg attctcctcg tccgagagca
    25141 tctccagctc tccagttacg agagtggatg gaagttcccg ttacgatcaa atctcccaca
    25201 ccagataagc cgctataggt caatggactg gccccgagtg ctacccctag gcgggtgatt
    25261 tctgctaaac ctcgagcgat gatggctgcc ttagcattat caccaaatcc aagaccatgt
    25321 aaagctccag caccgacagc aataatattt ttaagagcac cagcagtttc aaccccgata
    25381 acatccgtat tggtataaag tcggaagtag tgattactaa atagctcctg aacgtattga
    25441 gctgtttgta aatctttaga agcagcagtg attaaagtta ggtcacgcac aatggtttct
    25501 tccgcatgac taggccctga aacaacgaca atatcactgc ggagctgttc aggaatttct
    25561 tcttcaagga tagttgataa tcgtttatgg ctatcaggtt ctaatccctt tgaagcgtgc
    25621 atgatgatag ccttatggtc caaggtttgt gcaacttgtt gggcaacaag tcgtgtcact
    25681 tttgttggga caacaaacaa aatcgcatcc acatctttca atgtttctgc taagtcggtg
    25741 taggcaatga tattttcatc tagaacgaca tctttaaagt agtgcttgtt agtatgatag
    25801 gtattaattt cattgatttg ctcgggaaga tttccccaaa tacgtacctc gtgtccattg
    25861 tcatttaaga cttgtgaaag ggcagttccc caagaaccag gccccaagac ggcgacggtt
    25921 tgtttttcca tgatttcctc cttgatagag ttctttccat tattctatca tattctagag
    25981 gattttgcac ttgcattact ggggtatggt ggaaaaatac acaaagaccg agatgaaggg
    26041 ataaactctt agtctttagt taaagtgaga agaaatctga tagatagttt gattcagtat
    26101 atgctataaa tagaggtaaa caactcagga ggttcttgta ggttgcgaga gtttggcgaa
    26161 aaaattaaaa gattacgttt ggctaaaaaa atcagtcgtt cagaattttg tggtgatgag
    26221 tctgaattaa gtatccgtca attaattaga attgaaaatg gagaatccag accaacacta
    26281 acaaagttaa aatatatcgc tgaacgtttg gggggggggg ggaagattac aagttgatgc
    26341 taagttatat agagttggat aaggaatacc tagaattgaa gtatttcttg atgaggactc
    26401 ctacatacga agatgaaact atcgcccaaa agaaagagag tgtttttgct aagatttttg
    26461 aagagtatta tgataggcta cctgaggaag aaagatttat catcccaaat tattcatatc
    26521 tagcactagc gaactacaca gttcaaaaat taccagaaaa gctagttgaa atactgtcct
    26581 tctggtgatt ttgcttaatt tctgatgttc gagacatgat tgaggagatt tgtcttgatt
    26641 ttttcctaaa aaagggcaga ctcctccctt ggtttgatgc tcgattttta aaataaggtg
    26701 acttattctg ttaggcatgt tttcccctgc cctctagctc tatatggaac agggagtatc
    26761 agatttatct ggcagattgt atcaaataag gcttgaaact gttttgaata ggcgtataga
    26821 cttgagaatt tgagaatatg tcgtctagca gtagagacat attttccggc aatatgaagg
    26881 aagagacgtc gaaaacgctt gatagtcaag gactttactt catcaccagc tagctgcttt
    26941 aaaaagaggt agagattata agccagacag cccatcatca tacgtacttc attcttaatc
    27001 atggtcgaac tatctgtctt atccccaaag aatcctgctt tcctttcttt gataaagttt
    27061 tcagcattcc ctcgttcacg atagaattga aacagctcct cactatctac actgcttaaa
    27121 ttgttcaaat gaactttttt acaaccataa aaaagagcac ttaatcgtac tcttctcttt
    27181 taaggattag ttgaattaat tcaaatccac tagttctcct gtttctaggt agactaggcg
    27241 ttcacaaatg ttagcaatgt aatcagcgaa gcgttccaga tgtcctatga tataaaggta
    27301 ttgagttcca ttgggaattg aggtttcttg gtctttcata agtccaatga tttcctttga
    27361 taaggcataa taatattggt caatctgttc atctttttga gcaatactaa tagcttttga
    27421 ggcttggtgc aaaggaaagg caaccaataa atcggctagc atgctgaggg ataatttacc
    27481 catttggtgt aactgttctt cgtcaggggc tagttgattt tcttttagtt gcaaaacagc
    27541 tttggcaatg cctgccatat ggtctcccat acgttcaagg tctgaacaag aagacatgat
    27601 gctaatcaca aatcgaaggt cagacacttg tggctgttgc aaggccaaca aacgggcaca
    27661 ggtcaattcg atagcgcttt gaccttggtt gatagcatga tccttattga taattagctc
    27721 tgccatctcc ttgtctttgg aggctaaggc cagtaaggct tttgaagctg tttcaaggac
    27781 aagttgccct agtcctaaaa aggattgttc taattcatgc aattctaagt caaattgatt
    27841 tctcataggt ttttccttcc ttatccgaac cgtcctgaaa tatagtcttc tgtgcgctga
    27901 tcttttggat tggtaaacac gtcaacggta tctccaaatt cgcaaatttc tcctgttaag
    27961 aaaaaagcag ttttatctga aatacgtgaa gcttgttgca tgttatgggt aacaatgata
    28021 atcgtataat ccttttttag ttgctgaatg aggtcttcaa tttttaaagt ggagataggg
    28081 tctaaggctg aagtcggctc atccattaac agaacatcag gttctactgc taaagctcgc
    28141 gcaatgcaaa ggcgttgctg ctgaccgcca gataaggaca tggcactctt tttaagatca
    28201 tctttgactt cttcccaaat ggctgcccct tttaaagatt tctccactaa ggcatctaat
    28261 tgttttttgt ctcgaatacc atgtgtcctt ggaccataag ccacgttatc atagatagac
    28321 atggcaaagg gattaggctg ttgaaaaacc atccctacac gcttgcgtag ctgattaagg
    28381 ttgaatttgc tactataaat atcttgctca tctaagagga cttggccttc aatatggcaa
    28441 gaaggaacca aatcgttcat ccggttaagg gtttttagaa aagttgattt gccacaacca
    28501 gatgggccta tcaaggcagt aatctgtctt tctggtaatt gaatcgaaat attttttaag
    28561 gcttgaaaat ccccgtaaaa taagtctagg tgtctgactg aaaatgttcc catactagga
    28621 agctcctttc acaagttttc gagataataa gcttgataga gtatttatca ttaaaacagt
    28681 aataatcaaa atcacaccgg tagcataggc ttcattgaca tgtagcccct cacttgacag
    28741 catatacata tgcagggcta gagaacgacc tgaagacatg agactacttg gcgtattggt
    28801 agaggtacct aatgtataca tgagggcagc tgtctcacca acgatacggc caatagctag
    28861 tatcactcca gctaaaatac ctggcatggc aactggtaga acaattctaa aaacagtccg
    28921 taacttacct gccccaagtc cataacttgc ttgacgcatg ctatcactaa cagataaaag
    28981 ggcttcttct gttgagcgaa taatgactgg caacaccatg atgactgagg ttaagattcc
    29041 tgataacaga gagtattgaa aacctaagaa gactacaaag aagagcatgc caaacagacc
    29101 aaaaacaatg gaaggaatcc cagacaaggt atctgaggcc aatcgcatga ttttaacaca
    29161 aagggaatct ttttttgtat attccacaag ataaaaacca gcaaaaatcc ctatgggcaa
    29221 ggctaaaaga agagcaccaa agaccagaat aacggtggaa ataatcgctg gcataaggga
    29281 aatgttctca gaagtataag tccaagaaaa gagggataga cttagatgag gtaagccttt
    29341 gatgaggata aaaccaatga ttaaaaagag agagccaaag gttaaagctg aaaaacaata
    29401 aacgagaagt tttagcaggt atttactcat aagatgattt tcctttcaag taggcaaagt
    29461 aggcattaat caagagaata aggaaaaaga gaactgctga ggttgcaata agggcttccc
    29521 tatgctgacc tgatgcgtaa gccatttcca gaacaatatt ggttgttaag gttctggttc
    29581 ctgaaaagag tccacttgga ataatcggct ggttgcctgc caccaaaata actgccatgg
    29641 tttcacctac tgcgcgaccg attcctaaaa taactgctga taaaatacca gatctcgcag
    29701 ctggcaagat gacactaaaa atactccgtt catgactagc tcctagagcc aagctaccag
    29761 aataatacgt tttgggaact gttcggatag cagattctga caaactgata atggttggca
    29821 aaatcattat tcctaatagt aacgaagcgg ttaggacact catgccattt cctaaaaagg
    29881 ttctaatcca aggcaccaat aattgtaggc cgaaaaaacc ataaacaata gatggaatgg
    29941 ccgccatcaa gttgatagct gattttaaga agccatagac gggctttgga caataataaa
    30001 ccataaacac cgatgtcaag atgcctgttg gcaccccaat cacaatcgct cctaaggtaa
    30061 ttaataagga accaacgatc attggtaaaa taccatagct tgccggaatg ttcgttggcg
    30121 accaatcact gcctaataaa aaacgggcaa agccgtagtt agccatgaaa ggtaagccat
    30181 tactaaaaat aaagaaacag attagcaaaa tagctacaac agctactgtt gcactcatga
    30241 aaaaaattgc cctaaaaact gcttctttga aggcttgttt tgtcacatct tgtcctttct
    30301 agtgaagaaa gtaagggaga tacgacacct ccctacttgc cttctttatc ttattgtacg
    30361 atgaaacgtc tgcatctctt tagagattta tggagcaaac attttattta atcttgtccc
    30421 aggtggttaa tttgccacta aaaacgtctg caagttcagc catactgact tggcttgcct
    30481 tattgtcatt attgaccaca acagcaatac cgtctaaagc aatagcatca tgggtgagac
    30541 tcttaccttc ttcaggagtt aattccctag aaaccatacc aatatcagcg gttttctcct
    30601 taacagcggt aatacctgct gaagacccat tagaggtaat atcaatcgta acttctggat
    30661 tttctttttt ataagcttct gctaattttt ccattaaaga agatactgaa gtggaaccta
    30721 caacagacaa cttgcctgat aagtgttggc ttgtatattc tgtggtttcg gttttagctt
    30781 caataaattt attatctgtg accacttgtt gaccttgttt ggagtggata aagctgataa
    30841 aatcttgacc tagcttggaa agattagaag accaaacaat gttgaaggga cgttgaagag
    30901 ggtattcacc atctaaaact gtgtctcgac tagccttgac accatcaatc tctaaagcct
    30961 tgacagattt cgttaaagat cccaaggaga tgtagccgat agcattagca ttcccttgaa
    31021 ctgctgagag aacaccttct gtactatttt gaatcacagc tgttttggca gtgttgtcaa
    31081 tttttttatc accgtctttt ttgagaatcc ctgtgatttc tgtgaaggca ccccgtgttc
    31141 cagagccatt ttctcgtgaa atcacctcaa tcgttcctga agctgactgt ttggaagcag
    31201 ctgactgatt gccacaggca acaagcccaa atcctgataa gccaatggct gcaagagtaa
    31261 gcattttttt gaatttcata ataatcacct ttatctctat gtatttttct tgtgtaggct
    31321 tactacattt atagtctaac aagtctttgt aaaggtttat ccctgattca tgtaaagatt
    31381 gtgtaaagaa tcaaaaaaag ccacttttga aaaatggctg ctcctaaaaa tagctttaaa
    31441 aattattagt cctgtgcgaa agattggtta ggaagaaaaa tcgtgaagca actgcctctg
    31501 ccaagctgac tcgtcaccgt gacttggcca cctaataatt gactgagttc tttgacaatg
    31561 gcaaggccaa gaccagtgcc accagtttgt ctgcttcgac ctttattaac tcggtaaaaa
    31621 cgttcaaaaa tacgatcctg ctctaattga ctaataccaa tccctgtatc tgatacagaa
    31681 atcttaatgc cttcgttcac cttttgggtc ttgacctcaa tttttccccc ttgttcagtg
    31741 taacggatgg cattggataa aagattgagt aagatttggg aaagtaattg actatctgat
    31801 acgagggtga catcatctgg cacctgcacc tttagctgta aatccttctt cttgagctga
    31861 ggttgcaagc tttgagtcaa atcctgtaca aattctgcca aagaaagggt cgtccattgt
    31921 ataggcattt gttgagcctt agataaggta agaagatgct caacaatatg ctcaagacgc
    31981 aagctttctt tgtaaataat gtctagaaag tcatccttga gcgcttcttc ttcagctgac
    32041 atccccttaa tggtttcagc aaagccctta atcgaagtaa ctggtgtcct caattcatgg
    32101 gaggcatttg agacaaaggc taaatttaac ttttcataag ttctaatcgt tgttaaatca
    32161 tatagcaaga cgagcacagc ttccacagat tgggtggggc taaaaacggg aactgctgtc
    32221 acttctaaaa tcaagtcacc ctcatgaaac ccacttactt cttgttttaa ccttgttttt
    32281 tgatcaaagg cttggtgaac taaatttcga atatccatcc gtttgaggtc atcaagtgaa
    32341 cttatgtcgc cgtccacatc gggaaaataa tgaggcagag agcgactgga taataacatc
    32401 tgaccttgag cggaaactaa aaacgtcccc atggttaggt gcgacagaag aacctccatt
    32461 gtttcggcta ggtccttgta ttgctgatcc tgttgggaga ctttggtttt tagggcagac
    32521 acatactgag ccaaagactt taagtcttct tgcccttttt ctaaaaagta ttcactactg
    32581 gtcaagagag gttggtgcaa ggtctcaaaa gcaacttccc atttccaaag gcaaaagagc
    32641 cagtagccac cttgtcccaa agaaagggct agaagaaaga gaccgatgcc tttactgatc
    32701 caagttaatg ccatccctgc aatcagaatg aggctaacac ttagattgac tagccaaaat
    32761 tgaaggtagc gtttcatcta taactccttg aacttataac cataaccccg aatggttcga
    32821 ataaattgag gggctttagg attgtcttca attttttccc tcaacttacc aatatgaacg
    32881 tccaccaaac gtgtttcctg cccaaagtca tacccccaga tacgttccaa aagacgctct
    32941 ctagtcagtg tcatgttggg atgtttcata agatagagca agagttcaaa ttcttttggg
    33001 gtcaaactca gtaacttatt cgccttgtag acttcatgac gctcagggta tactttcaag
    33061 gtcccaaata gccaagaatc gtcagcgata ttatctgaat catctccttc ttgttctcct
    33121 ttagttcgcc tgaggacagc cttgacacgc gccagcaatt ctctagggct aaaaggcttg
    33181 gtcaggtagt catcagcccc taattccaag gccaaaacct tatcaaattc atcacttttc
    33241 gcagaaacca tcataattgg agttttgacg cctttggctc tcagccgctt acaaacttcc
    33301 atgccatcta attgtggtaa catgatatca agcaagataa aatcaaaggg ttctgtttct
    33361 gccaaagcta aggccttccg tccatttgtt accaattgag tagaaaagcc ttccttactt
    33421 aaatggtagt caagcaattt cagaatgtgt tcttcatcat ccactaataa gacttgtttt
    33481 gtcatctatt atctcctatt ggtaacatta taacacaatt atcagaaatc ctaacattgc
    33541 taaatcagat taaatttgct tatcaagact agtatctggt caaacgctca atcatctcct
    33601 tgtgctctgg ataggtcgcc agtagatcta ccctttcaaa taattcaaaa tcctcaaatt
    33661 caaaaccagg agcaacaaga caagaaacca gagcataatc cttatcaact gttgatcccc
    33721 aaatagtgcc cttaggaaca cagtagtgaa gttgttgccc tttggatatg tccaggccta
    33781 aagtgactgc ttcgtagtga ccatctgctg taatcatgtg aacagtaagt ggggatcctg
    33841 catgaaaata ccagatttca tctgctgtca atcggtgaaa atgtgaagga ttcgtttctt
    33901 ctaataagaa ataaatactg gtataaagcg cccttccctt accagcaagg tttatagtgt
    33961 ctgaagcttt ttttgtttgt ctaaaatagc caccttcaac atggggagct aactctagag
    34021 ttcttatcaa gtcttctt
//
LOCUS       DAVBCW010000010        32799 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVBCW010000010 DAVBCW010000000
VERSION     DAVBCW010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32799
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.010"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            218..448
                     /locus_tag="VQ514_000422"
     CDS             218..448
                     /locus_tag="VQ514_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HET2499237.1"
                     /translation="MESIGIGLVIVSHSKHIAEGVVELISKVAKDVPITYVGGTEGGG
                     IGTSFDQVDRVVSENPADTLLAFFDLGSAIKC"
     gene            852..1565
                     /locus_tag="VQ514_000423"
     CDS             852..1565
                     /locus_tag="VQ514_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HET2499238.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFAHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYNLYAATNGITAIQTGRLAQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            1623..2225
                     /locus_tag="VQ514_000424"
     CDS             1623..2225
                     /locus_tag="VQ514_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499239.1"
                     /translation="MSEVDFNEAVNYEFTSDTCQLANSIYQSLFKFFDKKNFSGDLIF
                     TWKSPSLVKEGDYIGRRDSQVDNLRVIGNIFPNYLTNRKYSLNMNRNGCMGDFPHDFF
                     DIYLDHVAKYAYEQKVNNIKEYYPLKRAILHQENALYFRFFSNFDDFLEKNYLKTIWQ
                     VSKETPFSEMDFNMFKNISEKIIFERGSKMLNDLKSNYKK"
     gene            2361..3014
                     /locus_tag="VQ514_000425"
     CDS             2361..3014
                     /locus_tag="VQ514_000425"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET2499240.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            3024..3488
                     /locus_tag="VQ514_000426"
     CDS             3024..3488
                     /locus_tag="VQ514_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HET2499241.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            3501..4769
                     /locus_tag="VQ514_000427"
     CDS             3501..4769
                     /locus_tag="VQ514_000427"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HET2499242.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLG
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRLVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            4783..5043
                     /locus_tag="VQ514_000428"
     CDS             4783..5043
                     /locus_tag="VQ514_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499243.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            5054..6415
                     /gene="pdrM"
                     /locus_tag="VQ514_000429"
     CDS             5054..6415
                     /gene="pdrM"
                     /locus_tag="VQ514_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HET2499244.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPTILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDKIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTARFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKR"
     gene            6569..7537
                     /locus_tag="VQ514_000430"
     CDS             6569..7537
                     /locus_tag="VQ514_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HET2499245.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            7553..8545
                     /locus_tag="VQ514_000431"
     CDS             7553..8545
                     /locus_tag="VQ514_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HET2499246.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDKNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYISEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            8763..9806
                     /locus_tag="VQ514_000432"
     CDS             8763..9806
                     /locus_tag="VQ514_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HET2499247.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            9870..11555
                     /gene="lpdA"
                     /locus_tag="VQ514_000433"
     CDS             9870..11555
                     /gene="lpdA"
                     /locus_tag="VQ514_000433"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET2499248.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEIIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMQSPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDAEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEIIGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            11619..12608
                     /locus_tag="VQ514_000434"
     CDS             11619..12608
                     /locus_tag="VQ514_000434"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HET2499249.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINHIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(12681..13751)
                     /gene="xerS"
                     /locus_tag="VQ514_000435"
     CDS             complement(12681..13751)
                     /gene="xerS"
                     /locus_tag="VQ514_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HET2499250.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVNDEQKNALDSL"
     gene            complement(14335..15885)
                     /locus_tag="VQ514_000436"
     CDS             complement(14335..15885)
                     /locus_tag="VQ514_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HET2499251.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFIIIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGSLVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
     gene            complement(15901..16680)
                     /locus_tag="VQ514_000437"
     CDS             complement(15901..16680)
                     /locus_tag="VQ514_000437"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HET2499252.1"
                     /translation="MATIKEIKELLVTVKELESPIFLDLEKDNRSGVQKEISKHKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQVVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            complement(16667..17518)
                     /gene="ylqF"
                     /locus_tag="VQ514_000438"
     CDS             complement(16667..17518)
                     /gene="ylqF"
                     /locus_tag="VQ514_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HET2499253.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            complement(17725..17901)
                     /locus_tag="VQ514_000439"
     CDS             complement(17725..17901)
                     /locus_tag="VQ514_000439"
                     /inference="COORDINATES: protein motif:HMM:NF012948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET2499254.1"
                     /translation="MQAGIPTDNEVSYALIYQQETLPATGSSTSALTALGLLAVGSLV
                     LLVHNMTGTVFCSL"
     gene            complement(17953..23844)
                     /gene="iga"
                     /locus_tag="VQ514_000440"
     CDS             complement(17953..23844)
                     /gene="iga"
                     /locus_tag="VQ514_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin A1 protease"
                     /protein_id="HET2499255.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGMGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYREETIETKIDFQEEI
                     QENPDLAEGTVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPSPRIVEKGTK
                     KTQVIKEQPETGVEHKDVQSGSIVEPAIQPELPEAVVSDKGEPEVQPTLPEAVVTDKG
                     ETEVQPESPDTVVSDKGEPEQVAPLPEYKGNIEQVKPETPVEKTKEQGPEKTEEVPVK
                     PTEETPVNPNEGTTEGTSIQEAENPVQPAEESTTNSEKVSPDTSSENTGEVSSNPSDS
                     TTSVGESNKPEHNDSKNENSEKTVEEVPVNPNEGTVEGTSNQETEKPVQPAEETQTNS
                     GKIANENTGEVSNKPSDSKPPVEESNQPEKNGTATKPENSGNTTSENGQTEPEKKLEL
                     RNVSDIELYSQTNGTYRQHVSLDGIPENTDTYFVKVKSSAFKDVYIPVASITEEKRNG
                     QSVYKITAKAEKLQQELENKYVDNFTFYLDKKAKEENTNFTSFSNLVKAINQNPSGTY
                     HLAASLNANEVELGPDERSYIKDTFTGRLIGEKDGKNYAIYNLKKPLFENLSGATVEK
                     LSLKNVAISGKNDIGSLANEATNGTKIKQVHVDGVLAGERGVGGLLAKADQSSIAESS
                     FKGRIVNTYETTDAYNIGGLVGHLTGKNASIAKSKATVTISSNTNRSDQTVGGLAGLV
                     DQDAHIQNSYAEGDINNVKHFGKVAGVAGYLWDRTSGEEKHAGELTNVLSDVNVTNGN
                     AITGYHYTGMKVANTFSSKANRVFNVTLEKDEVVSKESFEERGTMLDASQIVSKKAEI
                     NPLTLPTVEPLSTSGKKDSDFSKIAHYQANRALVYKNIEKLLPFYNKSTIVKYGNLVK
                     ENSLLYQKELLSAVMMKDDQVITDIVSNKQTANKLLLHYNDHSSEKFDLKYQTDFANL
                     AEYNLGNTGLLYTPNQFLYDRDSIVKEVLPELQKLDYQSDAIRKTLGISPEVKLTELY
                     LEDQFSKTKQNLGDSLKKLLSADAGLASDNSVTRGYLVDKIKNNKEALLLGLTYLERW
                     YNFNYGQVNVKDLVMYHPDFFGKGNTSPLDTLIELGKSGFNNLLAKNNVDTYGISLAS
                     QHGTTDLFSTLEHYRKVFLPNTSNNDWFKSETKAYIVEEKSTIEEVKTKQGLAGTKYS
                     IGVYDRITSATWKYRNMVLPLLTLPERSVFVISTMSSLGFGAYDRYRSSDHKAGKALN
                     DFVEENARETAKRQRDHYDYWYRILDEQSREKLYRTILLYDAYKFGDDTTSGKATAEA
                     KFDSSNPAMKNFFGPVGNKVVHNQHGAYATGDGVYYMSYRMLDKDGAITYTHEMTHDS
                     DQDIYLGGYGRRNGLGPEFFAKGLLQAPDQPSDATITINSILKHSKSDSTEGSRLQVL
                     DPTERFQNAADLQNYVHNMFDLIYMMEYLEGQSIVNKLSVYQKMAALRKIENKYVKDP
                     ADGNEVYATNVVKELTEAEARNLNSFESLIDHNILSAREYQSGDYERNGYYTIKLFAP
                     IYSALSSEKGTPGDLMGRRIAYELLAAKGFKDGMVPYISNQYEEDAKQQGQTINLYGK
                     ERGLVTDELVLKKVFDGKYKTWAEFKTAMYQERVDQFGNLKQVTFKDPTKPWPSYGTK
                     TINNVDELQALMDQAVLKDAEGPRWSNYDPEIDSAVHKLKRAIFKAYLDQTNDFRSSI
                     FENKK"
     gene            complement(24053..25231)
                     /locus_tag="VQ514_000441"
     CDS             complement(24053..25231)
                     /locus_tag="VQ514_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499256.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLIFNGIGRSNC
                     VCRNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLPLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWILNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
     gene            complement(25401..29051)
                     /gene="addA"
                     /locus_tag="VQ514_000442"
     CDS             complement(25401..29051)
                     /gene="addA"
                     /locus_tag="VQ514_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HET2499257.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMAERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKETFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEICSLNQESDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FAKLGQLSDQITILDYQERYHQDTWKLAKTFQTFMSHFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNKHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALVEKLKQFMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLESKEYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIDEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQIIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTALGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            complement(29048..32323)
                     /gene="rexB"
                     /locus_tag="VQ514_000443"
     CDS             complement(29048..32323)
                     /gene="rexB"
                     /locus_tag="VQ514_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HET2499258.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPVKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQITLVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQTTEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDQQGLENPAIPISPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWLEKIREE
                     LNR"
     gene            complement(32523..32678)
                     /locus_tag="VQ514_000444"
                     /pseudo
     CDS             complement(32523..32678)
                     /locus_tag="VQ514_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
BASE COUNT         9300 a         6452 c         6757 g        10290 t
ORIGIN      
        1 cttaacagat agtgtaaata aagataaact atttactggc taattaatca gttaaacact
       61 agttaaggag taatgatgaa aaaaagaaca atactattat tgatggccag tctgttagct
      121 cttgtcttag gagcatgtgg tttcttggac atattgatcc tggatcattc tcatcaggat
      181 tactctttac tgctatttta gaaactgggg tggtttgatg gaaagtattg gtattggtct
      241 tgttatcgtt tcacattcca aacacattgc agaaggtgtt gttgaactga ttagtaaagt
      301 agctaaagat gttccgatta cttatgtagg aggaaccgag ggcggaggaa ttggaacgag
      361 ttttgatcaa gtagataggg ttgtttccga aaatccagca gatactttac ttgccttttt
      421 tgacctaggt tctgctataa aatgttaagt aaatgatttc aaaatcagaa agggattgct
      481 ttgggcagtt cctttttaat gtaaatgtaa aaggagtaga atcatagtgt ttctaaattg
      541 tgaatcaatc aaaactgatt gtgatggggc tattctagct ttagaatcct tcaaaaatta
      601 aaatttaagg caatcaatta cttggaagag tatgaaagca tttagtttat aggaattcta
      661 ggcctagaat tacatatgta tttatgaaga taggggtgtt cgatagttag tattgttctg
      721 ttctgaaaga tttgagctgt cagttgtata gaaagtgttc gaattttttt taagtgatta
      781 aattagttaa ttgtatgagg ttctttatga tataatgttc ttaatgaatt ttcagaaagg
      841 aaaacctcaa attgttctac aaatttctac tcttcgacct cgaccacact cttcttgatt
      901 ttgatgctgc tgaggatgtg gctttgaccc aacttctaaa agaagaagga gttgcggata
      961 ttcaggctta taaagattat tacgttccta tgaacaaggc tctctggaaa gacttggagc
     1021 tgaagaaaat cagtaaacaa gagctggtta acacgcgctt ttctcgttta tttgctcatt
     1081 ttggacagga aaaagacggt agttttcttg cccagcgtta ccaattttac ctcgcccagc
     1141 agggacaaac actatcgggc gctcatgatc tcttggacag cctcattgag cgtgattata
     1201 acttgtatgc tgcgacaaat ggcattactg ccattcagac aggacgtttg gctcaatctg
     1261 gtctagcacc ttatttcaat caagtcttta tctcagaaca gttgcaaact caaaagccgg
     1321 atgctctctt ttatgaaaag attggccagc aaattgctgg atttagtaaa gaaaagacgc
     1381 tgatgattgg agattctcta accgccgaca ttcaaggtgg caataatgcg gggattgata
     1441 ctatctggta taatcctcat cacctcgaaa atcacacaca agcccagccg acttacgaag
     1501 tctattctta ccaagacttg ctggattgtt tagataaaaa tattcttgaa aagatcacat
     1561 tttaaaggag acgagctaat gactacaaaa aagctaatat tactattgaa gagtacattg
     1621 aaatgtctga agttgatttt aatgaagctg ttaattatga atttacatct gacacttgtc
     1681 aattagcaaa tagtatttat caatctcttt ttaagttttt tgataagaaa aatttctctg
     1741 gcgatttaat ttttacttgg aaatctccat cattagtcaa agaaggggat tatattggga
     1801 gaagggattc acaagtagat aatcttagag taataggaaa tatatttccg aattatctta
     1861 ctaatcgaaa atatagcctc aatatgaatc gtaatggctg tatgggagat tttcctcatg
     1921 acttttttga tatataccta gatcatgtag caaaatatgc ctacgaacaa aaagttaata
     1981 atattaaaga gtattatcct ttaaaaagag cgattttaca ccaagagaat gcattgtatt
     2041 ttcgattttt ttctaatttt gacgactttt tagaaaaaaa ttatttaaag actatatggc
     2101 aagtttctaa agaaactcct ttttctgaaa tggattttaa tatgtttaaa aatatttcag
     2161 aaaagataat cttcgaaaga ggtagtaaaa tgcttaatga tttaaaaagt aattataaaa
     2221 aatagccagt ctttataaac tgtattcacg tattcaaatc gtatatagtc gatttattaa
     2281 atctttagat aaactgtaaa aagtggtttc catagccact ttttgctata atagaggcag
     2341 taaaaatgaa ggagtcggct atggaacact cgtcttggca tgctttgatt aaggcgcaat
     2401 tacctgaggg ttatttcggg aaaatcaatc agtttatgga gcaggtctat tctcagggga
     2461 ttatttatcc acccaaggaa aaggtttttc aggctctctt gacaacactg cttgaagaag
     2521 ttaaggtggt aattctaggg caagacccct atcacggacc aggtcaagcg cagggcttga
     2581 gtttttctgt acctgactct atcccagctc caccatcctt gcaaaatatc ttgaaagaat
     2641 tgtcagatga tatcggagtt aagaaatctc atgatttgac agcttgggct gagcaaggag
     2701 tcttgcttct taatgcttgt ttgactgttc ctgctggaca ggccaatggt catgctggtc
     2761 aaatctggga gccttttact gatgctgtga ttcaggtggt caatcatcta gatagaccag
     2821 tcgtttttgt actctgggga gcttatgcac gtaagaagaa ggccttagtt accaatcctc
     2881 atcacttgat tatcgaatca gcccatccaa gtcctttgtc ggtttataga ggattttggg
     2941 gttccaagcc cttttccaag gccaatgcat tcttaaaaga gacaggacaa gagccaatcg
     3001 attggcttag ataaggagaa aatatgcctc agttagcgac gatttgctac attgataatg
     3061 ggaaagaact gctcatgctc catcgtaata agaaacccaa tgatgtccat gaagggaaat
     3121 ggattggtgt gggtggtaag ctagagagag gagagacgcc ccaggaatgc gcggcgcgtg
     3181 aaatccttga agaaacaggg ctcaaagcca agccagttct aaaaggtgtc atcacttttc
     3241 ctgaatttac accagattta gactggtaca cctatgtttt taaggtgacg gagtttgagg
     3301 gcgacttgat tgactgcaat gaggggacgc tagaatgggt tccctatgac gaggttttga
     3361 gcaagccgac ttgggaaggt gaccacacct ttgttgagtg gcttttagag gataaaccct
     3421 tcttttcagc caagtttgtt tatgatgggg ataaattgtt ggatacccaa gttgatttct
     3481 atgaataaag gagaaagcag atgctactaa tcaaaaatgg tcgtgtaatg gatcctaagt
     3541 ctggtttgga tcaagtttgt gatgtcttag ttcaagatgg gaaaattatc aaaattgcgc
     3601 ctgagatcac ggaagaagga gcagaaacga ttgatgctac tggtcttgtg gttgctcctg
     3661 gtttggtcga tattcatgtg catttccgtg aacctggtca aacacataaa gaagacattc
     3721 atactggtgc cctggcagcc gctgcaggtg gttttactac tgtcgtcatg atggctaata
     3781 ccagtccaac catttcagac gtggagactt tgcaagcagt tctccagtca gctgccaaag
     3841 agaagattaa tgtcaagaca gttgcgacca ttactaaaaa ctttaatggt aaaaacttga
     3901 ctgactttaa ggcgctctta gaagctggtg cggttggttt ctctgatgac ggtattccgc
     3961 ttgagagcag taagattgtc aaggaagcca tggaggaagc caaaaaactc aataccttta
     4021 tcagccttca tgaggaagat ccaggtttga acggtgttct tggctttaat gaaaatattg
     4081 ctagagaaca tttccatatc tgcggtgcta ctggggtggc tgagtacgct atgatggcgc
     4141 gtgatgtcat gattgcctat gcaactaaag cccatgttca catccagcat ttgtctaagg
     4201 aagaaagtgt taaagtagtg gagtttgctc aggggttagg tgcagaagtc acagcagaag
     4261 tagcgccaca gcatttctct aagacagaag cacttctttt aacacaaggt agcaatgcta
     4321 agatgaatcc accgcttcgt ttggaatcag accgtcgtgc cgttatcgaa ggtctcaaat
     4381 caggtgtcat cacagttatt gcgactgacc acgcgcctca tcatgtagat gaaaaaaatg
     4441 ttgaggatat taccaaagcg ccatctggta tgactggctt agaaacatcc ctgtctctcg
     4501 gcttgaccta tttagtagaa gctggtgagt tgagcttgat ggaattactt gaaaaaatga
     4561 catacaaccc agccaagctt tacaactttg aagcaggtta cttggctgag aatggtccag
     4621 cagatatcac tatttttgat gccaaggctg accgccttgt ggactcccat tttgcttcca
     4681 aagcagctaa ttcaccattc atcggtgaaa ccttaaaagg gcaggttaaa tataccatct
     4741 gtaagggaca aatcgtctat caagcttgat aggagttaga atatgaatta ttttcgaaaa
     4801 cgtagggaga gacaagcgaa atctaatagt ggaatttact gtcccgcagc taatcgttcc
     4861 aaaattcaat atgaaactaa agaaaaagct gaccatgcgg ttcaatatga taacggaggt
     4921 ttagttagaa gctattattg tcgtacatgt gcgtgttggc atactacttc aaaatctaat
     4981 aaacctccgc taagtttgaa aaactatggt ctgaaactct ttggtttaga taaatcagaa
     5041 tagaaagaga agtatgtata agacaaagtg tttacgagag aagttagtat tatttttaaa
     5101 aattttcttc ccaatcctga tctaccaatt tgccaattat tctgcctctt ttgttgatac
     5161 tgcaatgaca ggtcaataca acactatgga cttggctggt gtatctatgg caaccagtat
     5221 ctggaatcct ttctttacat ttctaacagg gattgtgtca gccttggtgc ctatcattgg
     5281 tcaccatctt ggtcgaggca aaaaggaaga agttgcgtct gatttttacc aatttattta
     5341 tttggccttg ggcctatctg tggtcttgct ggggatggta cttttcttgg caccaacaat
     5401 cttgaatcat attgggttag aagcagcagt agcggcagta gcggttcgct atctttggtt
     5461 tttatctatc gggattatcc ccttgttgct ctttagcgtc attcgttcct tgctggattc
     5521 gctgggcttg accaaactgt ccatgtacct catgcttttg ttactccctc tcaatagcgg
     5581 atttaactat ctcttgattt acggtgcctt tggtgttcca gaactgggag gggctggtgc
     5641 tggtttagga acatccttgg cctactgggt cttgcttggg atttctgttc tggttttatt
     5701 taaacaggag aagctcaaag ccttacacct tgagaaacga attccactta atatggataa
     5761 aattaaggaa ggagttcgtt taggtctgcc tattggggga actgtcttcg cggaagtggc
     5821 tatcttttca gtggttggct tgattatggc taagttttcg tccttgatta tagctagtca
     5881 ccagtcagct atgaactttt caagtcttat gtacgccttt cctatgagta tctcatcggc
     5941 tatggctatt gtcgtttcct atgaagtggg agccaagcga tttgatgatg cgaaaaccta
     6001 tattggtcta ggaagatgga ctgccctcat ttttgcggcc ttcaccttaa ccttccttta
     6061 catttttagg ggaaatgtgg ccagtcttta tggtaacgac ccaaaattta tcgatttgac
     6121 agcgcgtttt ttaacttata gtcttttctt ccagttagca gatacctttg cggcgccgct
     6181 tcagggaatt ttgcgggggt ataaggatac agttattcct ttttaccttg gtttgcttgg
     6241 ttattggggc gtagcaatcc ctgtggctac gctatttgat tccctaacag attttggagc
     6301 ttattcttac tggatcggct tgattattag tttgattgtg agtggggcgc tctaccgttg
     6361 gcgtttaact gtgattatga agagatttga atctttagca aaatccaaac gctaaaaagt
     6421 ttgtgaaaaa atattcttga taagaaaatc ccttgttttc acggggtttt cttttttatt
     6481 ttgtgaaatt tcttttagaa aaactttgac agtatttgcc aaaaacggta aaatagtaag
     6541 gtaagaagaa agttagaaag gcaagaagat gtcaacttta gataaaaatc ttttgctaga
     6601 aatgttccgt aagatggaag aaatccgtcg catggactta aaaattgcac aattagtaaa
     6661 gaaagggaaa gtgccaggaa tgacgcactt ttctgttggt gaagaggcag ctaacgtggg
     6721 ggctatgtta gctctcaatc cagatgatct gattacctca aaccaccgtg gacacgggca
     6781 agctattgct aaagggatcg acctcaacgg aatgatggct gaaatccttg gtaaatacac
     6841 tggaacctgt aaagggaaag gtggatctat gcatatcgct gaccttgatg ctgggaacct
     6901 tggtgccaat ggtatcgtag gtggtggtat ggggatcgct gtcggtgcag ccctcagtca
     6961 gcaaatgcaa aataccggta aaatcgttgt ctgcttcttt ggagatggtg cgaccaatga
     7021 aggtgttttc cacgaagcag tgaacatggc ttctatctgg aacctgccag tcattttcta
     7081 ttgcattaac aacggttacg gtatctctgc ggatatcaag aaaatgacca atatagaaca
     7141 tatccatcaa cgtagcgccg cttatggaat tcctggaatg ttcatcgaag acggtaacaa
     7201 tgtcatcgat gtctatgaag gatttcagaa agctgtagac catgttcgca gtggcaatgg
     7261 tccagtcttg attgaaagtg taacttatcg ctggctcggt cactcatcat ctgaccctgg
     7321 taaatatcgt acgcgtgaag aagtggaatt gtggaaacaa aaagatccaa tcgaaaacct
     7381 ccgcaattac cttattgaaa ataacattgc aagtgccgaa gaattggaag aaatccaagc
     7441 gcaagtaaag gaagcagtag aagcttctgt taaatttgca gaggaaagcc cattcccacc
     7501 gcttgaatca gcctttgaag atatttacgc agactaagga gaaagagata aaatggaaac
     7561 aaaaacaatg tccttccgtg acaccattat ccttgctatg tctgaggaaa tgcgtcgcga
     7621 tgaaaatgtg ttcttgatgg gagaagacgt cggtgtcttc ggaggagact tcggtacttc
     7681 tgttggaatg cttgaagaat ttggtccaga acgcgtccgt gactgtccga tttctgaagc
     7741 tgccatctct ggagcagcag caggagcagc catgacagga cttcgtccaa tcgtcgatat
     7801 gaccttcatg gacttctctg ttattgccat ggacaatatc gtcaaccaag ctgctaaaac
     7861 acgttacatg tttggtggta aaggtcaggt tccaatgact gttcgttgtg cagctggtaa
     7921 cggagttggc tctgccgccc agcactcaca atctctagag tcttggttta ctcacattcc
     7981 aggacttaag gttgtggcac ctggaacacc tgcggacatg aaaggcctgc ttaagtcttc
     8041 tatccgtgat aaaaacccag ttattatact tgagtacaag tcagaattta accaaaaagg
     8101 ggaagtgcca gttgatccag actacacaat tccacttggg gttggtgaaa tcaaacgcca
     8161 aggaacggat gtaacagttg ttacttatgg aaaaatgctt cgccgtgtgg ttcaagctgc
     8221 tgaagaatta gcagaagaag gaatttcagt tgaaattgtt gacccacgta cccttgttcc
     8281 gcttgataag gatatcatca taaactcagt gaagaagact ggtaaggttg ttctggtcaa
     8341 cgatgcccac aaaacaagtg gctatatcag tgaaatttca gctattattt cagaatcaga
     8401 agcatttgac tatctagatg caccaatccg ccgttgtgca ggagaagatg tgccgatgcc
     8461 ttatgcacaa aacctagaaa atgcaatgat tccaacagtt gaaagtatca aagatgcaat
     8521 ccgaaaaact tataacaaag aatagagtta cataagataa gttatgtaaa taaaataaag
     8581 ctgacgagaa actttgtatc ttttaggtgc agagctcttc gtcattaata tcttatattc
     8641 gagcacgggc taaaagctcg gaaaaaagat aaatctcctt ggcttcatcg aagccatcgt
     8701 cgatttccta tttttcagtc gcttttgacg ccctttgtat cataaataga attggagagg
     8761 tcatggctga tgacaagcta agagcgactc ctgcagctag aaagttagcg gatgatttag
     8821 ggataaacct ctacgacgtt tctggctcag gtgcaaacgg tcgtgtccac aaagaagacg
     8881 tggaaactta taaagacaca aacgtggttc gcatttcgcc acttgcaaaa cgaattgccc
     8941 tcgaacataa cattgcttgg caggaaatcc aaggaaccgg tcatcgtggt aaaatcatga
     9001 agaaggatgt tttggccctg cttcctgaaa atatcgaaaa cgatagcatc aaatctcctg
     9061 ctcagattga aaaagtggaa gaagtccctg ataacgtaac accatatggt gaaatcgagc
     9121 gtattccaat gacaccaatg cgtaaggtta ttgcccaacg catggttgaa tcttacttga
     9181 ctgcgccaac cttcaccctc aactatgaag ttgatatgac tgaaatgttg gctcttcgta
     9241 agaaggttct tgagccaatc atggaagcaa ctgggaagaa gactactgta acagaccttc
     9301 tttcgcttgc agttgttaag actcttatga aacacccata catcaacgct tcattgacag
     9361 aagatggcaa gactattatc actcacaact atgtcaatct tgccatggca gttgggatgg
     9421 ataatggatt gatgacacct gttgtttaca atgctgagaa gatgagtctt tcagaactag
     9481 ttgtagcctt taaggatgtt attggccgta ccttggatgg taaattggct ccaagtgagt
     9541 tacaaaattc aacattcaca atcagtaatt tgggaatgtt tggtgttcag tcctttggcc
     9601 cgattattaa ccaacccaac tcagctatcc ttggtgtcag ttcgacaatc gagaagccag
     9661 ttgtcgtcaa tggtgaaatt gtgattcgcc caatcatgag tttaggatta accattgacc
     9721 accgtgtcgt agatggtatg gctggtgcta agtttatgaa ggacttgaaa gagttgattg
     9781 aaaccccaat ctcaatgttg atttaagagc aagagtattc atttttaaga tatagataaa
     9841 ggaaggaaga catggcctta gaaataatta tgccaaaagc cggcgtggat atgacagaag
     9901 gacaaatcgt ccaatggaat aaaaaagtcg gagaatttgt aaaagaagga gaaatccttt
     9961 tggaaatcat gactgataaa gtcagcatgg aattggaagc cgaagaagat gggtacttga
    10021 ttgccattct caaaggagat ggtgaaactg tccctgtaac ggaaattatc ggttaccttg
    10081 gtgaagaaag ggaaaacatc ccaacagctg gagcagcatc gccagaagct agccctgtac
    10141 ctgtagcaag tacctcaaac gatgatggta agagcgatga tgcttttgat atcgttgtga
    10201 ttggtggagg tcctgctggt tatgttgcag ccattaaagc tgcccaactc ggcggtaagg
    10261 ttgcccttgt tgagaaatct gaacttggtg gaacctgctt aaaccgtgga tgtattccaa
    10321 ccaagaccta ccttcataac gctgaaatta ttgaaaatat cggtcatgct gcaaatcgtg
    10381 gtatcgtgat tgaaaatcct aacttcactg ttgatatgga aaaactttta gaaactaaat
    10441 ctaaagttgt taatactctt gttggtggtg ttgcaggact tcttcgtagc tacggagtta
    10501 ctgttcataa aggaattggt acaatcacta aagacaagaa cgtcttggta aatggttctg
    10561 aattgcttga aaccaagaaa atcattcttg ctggtggttc aaaagtcagc aagatcaacg
    10621 tccctggtat gcaatctcca cttgtcatga ctagtgatga cattcttgaa atgaacgaag
    10681 tgccagaaag ccttgttatc atcggtggtg gagttgtcgg tatcgaactc ggtcaggcct
    10741 tcatgacatt tggttcaaaa gtgactgtta tcgaaatgat ggaccgtata gttccagcta
    10801 tggatgcgga agtttctaag aaccttcgct tgatccttga gcgtaaagga atgaccatct
    10861 tgactggtac taaactgcaa gaaatcattg aggaaaatgg tcaacttcgt atcaaggttg
    10921 aaggaaaaga cgatatcatc gcaagcaaag ctcttctttc aattggtcgt atgccagacc
    10981 ttgaaggtat tggagaggtt gagtttgaat tggatcgtgg ttgtatcaag gtcaacgaat
    11041 acatggaaac ttcagttcca ggcatttatg caccaggtga catcaacggt actaagatgt
    11101 tggctcacgc agctttccgc atgggtgaag tttccgctga aaatgccctt aaaggaaatc
    11161 atgcagttgc caaattgaat ttgactcctg cagccatcta cactctccct gaagtagcag
    11221 cagtaggttt gacagaagaa caagcccgtg agaaatacga tgttgccatc ggtaagttta
    11281 actttgctgc taacggtcgt gctattgcat ctgacgcagc tcaaggtttc gtaaaagtta
    11341 tcgctgataa gaaatacgga gaaatcattg gtgtacacat tattggtcct gcagccgcag
    11401 aattaataaa cgaagcatca agcatcatcg aaatggaaat cactgttgag gaaatgctga
    11461 agaccatcca cggacaccca acttactctg aagtgatgta tgaagcgttt gcagatgttc
    11521 taggaatggc catccattca cctaagaaaa aataaaacga agatagatta gactggaagg
    11581 ttatttccag tctctatcat ataaagtaaa gggatatcat gaaatacatt atcaatcatt
    11641 caaatgacac tgcttttaat attgccttgg aagaatacgc ctttaaacac cttttggatg
    11701 aggatcaaat cttcctactt tggattaaca agccatctat cattgttggt cgtcaccaga
    11761 acactatcga agaaatcaac cgtgattatg ttcgcgaaaa tggtattgag gtagttcgcc
    11821 gtattagtgg tggtggagct gtttaccacg atttaaataa cctcaactac acgatcatct
    11881 ccaaagaaga tgaaaataag gcatttgact tcaagagctt ctcaactccg gttatcaata
    11941 ccctggctca actcggggtt aaagctgagt tcacaggtcg taatgacctt gagattgatg
    12001 gtaagaagtt ctgtggcaat gcccaagcct atatcaacgg ccgtatcatg caccacggtt
    12061 gcttgctctt tgacgttgat ttgtcagtcc tcgcaaatgc ccttaaggtt tcaaaagata
    12121 aatttgaatc aaaaggtgtg aaatccgtcc gtgcccgtgt aaccaatatt atcaatgaat
    12181 taccaaaaaa aatcacagtc gaaaaattcc gtgatttact cttggaatac atgaaaaaag
    12241 agtacccaga gatgactgaa tacgtctttt cagaagaaga attggccgaa atcaatcaca
    12301 tcaaggatac taagtttgga acttgggact ggaactatgg taaatcacct gaatttaacg
    12361 tccgtcgtgg aaccaaattc actagtggta aggttgaagt ctttgctaac gttactgaat
    12421 caaaaatcca agacatcaag atttatggtg acttctttgg tattgaagac gttgctgcag
    12481 tagaagatgt ccttcgtggg gtgaaatacg aacgcgaaga tgttcttaag gcgctaaaaa
    12541 ccattgatat cacacgctac ttcgctggta ttagccgtga agaaatcgct gaagcggtag
    12601 ttggataaat caaaaagaag ttggccaact aaccaacttc tttttttgat atttatttac
    12661 ataatttaaa atacgtgaaa tcataaacta tctagagcat tcttttgttc atcattaaca
    12721 atatgggtat agaggtcagt gacttgtgtg ctggcatgtc ctagttggtg actgactaaa
    12781 acttgtgatt tagtcgcatc atagagccta gttgctagcg tatggcgcag tttatggggt
    12841 gttacacgta ctttaaaatc ctctgagtat ttagcaacca ttttctcaac gctagaagca
    12901 tcgatacgat taggaacacc tctgtagaga gttaaaaaaa gggctgtatc tgttttttcc
    12961 gttttatagc gttgattccg aatggccaga taattctcta aataaggttt agcaaaagca
    13021 gcgacattga ctgagtcacg tttgccacct tttcgagtaa catcaataac catcattttt
    13081 agattgagat ctcttagatc tagattaaca gcttcagata agcgaacacc agatgccaag
    13141 agaagggcaa taatggctaa atctcgttct ttatttttgt tgaatgatga gagagctcga
    13201 tttgaaagct gttgtgggtg ctcttgatcg atataagtta gaaaaccttc tgtttcatca
    13261 cctagaaaga gtttttgctt gatattttca gctctggcag caagggtttc tttctttttc
    13321 ttggttgaaa cttttttcat tacattacga tagaaataag gttccccctg atcgttttca
    13381 acctcctcgg ttagatactt gtaaagacta gaaagtgctg ataaggttcg attgatagtt
    13441 gtctgtgaaa caccttgttt tgttgtatta gcattcagca agggacgttc acgtagataa
    13501 aggataaagg attccatgtc tttcttagac atattttcca aaactgataa aggaatatcg
    13561 gatattttat cagcgtttga aataccagac tccaaaaccc agctgaaaaa tcggtcatat
    13621 tccttaaggt attcgtacag ggttgtaaaa ctgtagggca cagccagctt agattggtag
    13681 tattccagaa cataccaggg catgagttgt tttagtttgt cgattcgttc cagtaaaatc
    13741 tcacgtttca tgtattttct ccataagtaa gtatactagt agtatagcat agacttgtat
    13801 atttttcaag aaaattatag tttttcagaa agatgttata ggagtttttc aaagtgataa
    13861 aaagacttag acctacaaac aaaacagaat aaggaaaata ataaaaatct cacccactaa
    13921 acttctgtta atactacata actgaagagc tatataagga gctatgagat acaaaacaca
    13981 ttgtataata gattgaaact agaatagtac atctggactt ctaaaacatt gctagaaatt
    14041 gatttgattg ttctgattgt tttatcctat ttctatttca tttcactata taattgctat
    14101 tgttttcttt ttcatgattt taatcttact gtacagattt gatacagtaa actcctttct
    14161 agctttatac tttatcattt ctaacttaat tatagtctta tttttaataa agttcaatta
    14221 tttatatgta aaattcctca tcagtaaaaa ataaccgatc tcatttctga gaatcggttt
    14281 tctaaataaa tcaagctgag tatttacata acataaatta tgtaaatact catactacaa
    14341 caacaaatct ttgacttttc caatttcact ttttggaata accaggtgaa tcatatcacc
    14401 cagatacatt ctggttgagc cgttaactgt ttggctcttg ccattatgga cttgagttgt
    14461 gatgaggacg ttgtgtggta agttgagttc atgaacttgt ttcccagcaa ttttatcaga
    14521 aactggtatt tcgataagtg taacttctcc ttcgctagat acttcttctg gaagcatttt
    14581 ttccagcatg gcttcataga ctggcgtacc tttgagcaaa tccatgataa tataagaaac
    14641 aagagtgaca agaccaagtg gcatgaggtt gcgaatatct cctaccatct cagttacgag
    14701 gatcatagcg gttaagggag cttttgatat ggctccaaaa tagccactca ttcctagaat
    14761 gacaaatata gggaattgct cttgactgac aagtccaaga ttgacacaga taacaccaac
    14821 taaggaacca agcaaagaac caagagctaa aatggggagg aaaattcctc ctggcagtcc
    14881 acttccatag ctaatcatac tccaaataaa gcggatcaaa aagtaagcta ataaaacttg
    14941 gaaactaaaa ttttgttcag ttaaagaaag gacaagctga tttccgccac caatgatttg
    15001 aggtaagaag attccgactg gtatgataat gataaaagcc aagatgggat aataagccct
    15061 atccaaatgg attttttgac caatcaagtc ataaactctt ccaacgttta atacagcttt
    15121 ctcatagaga aaacctgaaa atccaaggaa aattcccatg acgagatata tccaatactg
    15181 gtctagggtc atgggaggaa tgttatctgg catatccaat actggtgtca aaccgaacat
    15241 gagtagagac acaaagtttg ctacgatgct ggctgctaga gttgagaccc agaaaaagcg
    15301 cgaaaaatgg tgatagactt cttctacaac aaagagaagt gctgcaatag gagcattaaa
    15361 ggctgcggct aaacctgctg cagctccact ggcaatcaag gaacgttcct ctactggact
    15421 ggatttgagc cacttggcaa ttcctttacc accaactgct ccaagttgaa tgctgggtcc
    15481 ctctcgaccc agcatgagtc cactggcaat agcaagaata cctagcacat attttttcca
    15541 aagaatgccc caccagttga gggacatgag gcctttcagt tcggcttcga cttgaggaat
    15601 tcctgagcct ttaatatctt tttctgaccg tgttagtttg gcactgagcc aacagatgag
    15661 tatataaaac aaaaccagta caaaaagatt gcgcactaag tacccttgat cttgataaac
    15721 tccttgtatc aggtggaagc ccttttcaat taagaaacga aaggatccga cgataattcc
    15781 aacgatgagg ccgacaatga ttcctcgacc aacttgggat agtatagtgc tggatgcaaa
    15841 ggcgaattct ttcttagaac ggactatttc cgactgttcc tccatggtat ttcctttcaa
    15901 ttaactttct tttttaccta aaaccagtga tttaacgggt tcaaagctgg ttcggtgaat
    15961 tggggtaact cctagttttg tgaggccttc cagatgttta gctgtgccat atcctgcatt
    16021 agtagcgaaa tcatagccag ggaactgctg atcgtattcc ttcatcaatt catcacgtgt
    16081 taccttggct actatagatg ctgctgcgat agagagggag ttggcatctc ctttgataat
    16141 ggaggtttgt gaaatgggca agtccagttt catggcatca atcaaaaggt gctctggttg
    16201 agggctgagc tgggagattg cttcttgcat ggctagtttg gttgcttcat agatattgac
    16261 ttggtcgatg acctgattat ctatgatacc aattccaatc gacaaggctt ggtcttgaac
    16321 gacttggaaa atctccagat gtttcttttt aggaattttc ttgctgtcgt tgagaccttt
    16381 aatcttacaa tttttaggta aaataacggc cgcagcgact acaggaccag caagaggacc
    16441 acgaccaacc tcatcaatac ctgcaattaa ggtcaatcct tgcttataaa gttctttttc
    16501 ataagaaagc atggattcca agcgcaaatt ttcatctaat tcagcttgaa tggctctttt
    16561 atgcttgctg atttcctttt gaactcctga gcgattatcc ttttcaagat ctaaaaaaat
    16621 agggctttct aactccttga ctgtcacaag gagttctttg atttctttaa tcgttgccat
    16681 cgaggtcttc caatgtatct aaggtatagt taccgagttt gccatcacgg acttccttca
    16741 cgaagagact gtaaaaacgg tcatagtcat cacggaaacc gagggcgcgg gtcatatcca
    16801 taataatcac aggcgcttct tcttcaattt tcatttgttt gaagcgttca gccagctttt
    16861 ctggataatg ttctttgaaa taattgatac caaaaatggt aacctcatcc ataggaagca
    16921 actgatcctt gatagctcca gtcaatgcca acttaagtgc aacagtttca tcctcaaact
    16981 taggccagag aatccccggt gtatccaaga tttccagatc tttattggtt ttaagccatt
    17041 gttgaccttt tgtgacccct ggcttgtttc caacaacagc aatcttttta ccagccaaac
    17101 ggttcatcag agttgattta ccagcgtttg gaatcccaat aatcatggta cgcaaggttt
    17161 caatctgaat cccacgttct ttctggcgag caatcttatc agccatgagc ttcttggccg
    17221 catctgttac aacttttaca gtcacttgct ctttggagtt gatagctagc gtctggattc
    17281 cttgtgattc aaaatactga cgccattcct tggtcattgc tggatcagcc aagtcggcct
    17341 tgtttaaaat caagagtttt ggtttatcac caacaatctt ggttaacata ggattttgac
    17401 tagatagagg taagcgtgca tcaactaaaa tcgtcacaaa atcaacaaat tttaaattct
    17461 cctgcacctg tcgacgagct ttagacatgt gaccaggaaa ccattgaata gtagccattg
    17521 agttttttcc tttcatagca aggatttaga tcccctattt tattttacta ttgtttgaac
    17581 atagagagtt ttcaaatgaa tgatgtccaa acagttcttg ttttatgaca tagaaatcta
    17641 ctttatatta tatcatgcct tctttatttt gtccgatgat agccttttac cctatataga
    17701 aaaaagccat caaatgatga cttttcagag ggagcaaaaa actgttcccg tcatattatg
    17761 aaccaaaaga actaaactac caacagctaa taggcctaaa gctgtaagtg cagaagttga
    17821 tgaacctgtt gcaggaagag tttcctgctg ataaataaga gcataactta cttcattatc
    17881 agtaggaatt cctgcttgaa tcttggtttg ttcttcatca cctttttaaa ctttatttcc
    17941 taatagtaaa cactattttt tattctcaaa aattgaactt ctaaaatcat ttgtttggtc
    18001 aagataggct ttaaagattg ctctcttcaa cttatgaacg gcactgtcga tttctggatc
    18061 ataattactc caacgtggac cttcagcatc cttgagaaca gcttggtcca tgagggcttg
    18121 caattcatcc acattattga tagtctttgt gccatagctt ggccatggtt ttgtcggatc
    18181 tttaaaggtc acctgcttca agtttccaaa ctgatccact cgttcttggt acatagctgt
    18241 cttaaattct gcccaagttt tatacttacc gtcaaatacc tttttcaaaa caagctcatc
    18301 ggtcaccaag ccccgttctt taccataaag attgatagtt tgcccttgtt gtttagcatc
    18361 ttcttcgtat tggtttgaga tataaggtac cattccatcc ttaaagcctt tggcagccaa
    18421 aagttcgtac gcaatcctac gtcccataag gtcccctggt gtgcctttct cactgctgag
    18481 agctgaatag attggggcaa agagtttgat ggtatagtag ccatttcgtt catagtcgcc
    18541 agactggtac tcacgagctg ataagatgtt gtggtcaatc aaactttcaa aactattcag
    18601 gtttcgggcc tctgcttctg tcaattcttt gactacgtta gtggcataaa cctcatttcc
    18661 atctgctgga tctttcacat acttgttctc aatttttctg agagccgcca ttttctggta
    18721 aacacttagt ttattaacga ttgactgccc ttcgagatat tccatcatgt agataaggtc
    18781 aaacatgtta tggacatagt tctgaagatc tgctgcgttt tggaatctct ctgtcggatc
    18841 caagacttga agacgggatc cctctgtact atctgatttt gagtgtttca agatagaatt
    18901 aatggtaatg gttgcgtcac ttggttggtc aggggcttgc aataagcctt ttgcgaagaa
    18961 ctctggtccc aagccatttc ttcgaccata gccaccaagg taaatatcct gatccgaatc
    19021 atgggtcatt tcatgggtat aagtaatggc tccatccttg tccagcatgc ggtaagacat
    19081 atagtagacg ccgtcccctg tagcgtaggc tccatgctgg ttgtgtacta ccttattgcc
    19141 aactggtcca aagaagttct tcatagctgg attggagcta tcaaacttag cctccgctgt
    19201 agcttttcct gatgttgtgt catccccaaa cttataggca tcataaagga gaatcgtacg
    19261 atagagtttt tcgcgtgact gttcatctaa aatacgatac caataatcgt agtgatctcg
    19321 ctgacgtttg gctgtttcac gcgcattttc ttcaacaaaa tcattgagag ccttgcccgc
    19381 tttatggtca ctactgcggt agcgatcata agctccaaat cctagactag acatggtcga
    19441 gatgacaaat acggatctct ctggcaaggt caggagaggc aagaccatat tgcggtattt
    19501 ccatgtggca ctcgtgatac gatcataaac accgatagaa tacttggtgc cagctaaccc
    19561 ttgcttcgtt ttcacctctt cgatagtgga tttttcttcg acaatgtaag ccttagtctc
    19621 tgatttaaac cagtcattat tgcttgtatt tggtaaaaag acttttcggt aatgttccag
    19681 cgtgctaaac aaatctgtcg ttccatgttg actggcaaga ctgataccat aagtatcgac
    19741 attattctta gctagaagat tgttaaagcc agatttacct aactcaatca gagtatctag
    19801 tggggaagta tttcctttac caaagaagtc tggatgatac ataactaggt ctttgacatt
    19861 cacttgacca tagttaaagt tataccaacg ttctaaataa gttaaaccga gtagcaaggc
    19921 ttccttatta ttcttgattt tatctacaag atagcctctg gtgactgagt tatcgctagc
    19981 tagaccggca tctgctgaca aaagtttttt caagctgtct cccagatttt gttttgtttt
    20041 ggagaactgg tcttctaaat agagctcggt taacttaact tctggagaaa taccaagtgt
    20101 ctttctgata gcatctgact ggtaatcaag cttctgcaac tcaggcaaga cttccttaac
    20161 aatagagtct cgatcatata agaattggtt aggagtgtag agaagtcccg tattacctag
    20221 attatattct gctagattgg caaaatcagt ctggtacttg agatcaaatt tctcagatga
    20281 atggtcatta tagtgaagta agagtttgtt tgcagtctgt ttgttggaaa caatatctgt
    20341 gattacttgg tcatccttca tcataactgc agataagagt tctttttggt ataagagact
    20401 gttctcctta accaggtttc cgtatttgac gatggttgac ttattataaa aaggtaacaa
    20461 tttttcaatg ttcttataaa ccaaagcacg gttagcttga taatgggcta tcttagaaaa
    20521 atcactgtct ttcttgccac ttgttgagag gggttccacc gttggtagag tgagaggatt
    20581 tatttctgct tttttgctta caatttgaga agcatctagc attgttcctc tttcttcaaa
    20641 ggattccttg ctgacgacct catccttctc taaagtgaca ttgaaaactc tattagcctt
    20701 actactaaat gtattagcta ccttcattcc tgtatagtgg tatccagtga tggcatttcc
    20761 gtttgttaca ttgacatcgc taagaacatt ggtcaattca ccagcgtgtt tttcctcacc
    20821 agaagttcga tcccacaaat atcctgccac accagcgact ttaccaaagt gcttgacatt
    20881 attaatatca ccttccgcat aactgttctg tatatgcgca tcttggtcta ctagacctgc
    20941 aagaccaccg acagtctgat ctgacctatt ggtgtttgac gaaatggtta ctgtcgcttt
    21001 ggatttagca atagacgcat tttttcctgt taaatgaccg accagaccgc caatattgta
    21061 ggcatcagtc gtttcatagg tattgacaat tcttcccttg aaactgctct ctgcgatgct
    21121 tgattggtct gccttagcca acaaaccacc gacaccacgt tctccagcca gaacaccatc
    21181 aacatgaact tgtttaatct ttgtgccatt cgtagcttca tttgctagtg aaccaatatc
    21241 atttttccct gaaatagcaa cattttttag actcagtttt tctactgtag caccactcaa
    21301 gttttcaaac agaggttttt tcaaattata gatagcataa ttcttgccat ctttttcacc
    21361 gattaaacga ccagtaaagg tgtccttgat ataggatctt tcatcaggac caagctccac
    21421 ttcgttagca ttcaggctgg ccgctaaatg ataggttcca gagggatttt ggtttatagc
    21481 tttgaccagg ttgctaaagg aagtaaagtt tgtattttcc tctttagcct tcttatcgag
    21541 gtagaaggtg aaattgtcga catatttatt ttctagttct tgctggagtt tctcagcttt
    21601 ggctgtgatt ttataaactg actgcccatt tcttttctct tcagttattg aagctactgg
    21661 tatatagaca tctttaaatg ctgaagattt taccttgaca aagtaagtat ccgtattctc
    21721 tggaattcca tccaatgaaa catgttgtct gtaggttcca ttcgtctgac tatacaactc
    21781 aatatcagaa acatttctta attcgagttt tttctctggt tctgtttgtc cattctctga
    21841 tgttgtatta cctgaatttt ctggttttgt tgcagttccg tttttttctg gttgatttga
    21901 ttcttcaact ggtggttttg aatcactagg tttattggat acttctccag tattttcgtt
    21961 agctattttc ccagagtttg tttgtgtttc ttctgcaggt tgaactggtt tttctgtttc
    22021 ttgatttgag gtaccttcta ctgtgccttc atttggattt actggaactt cttctacagt
    22081 tttttctgaa ttttcatttt tagagtcatt atgttctggt ttatttgatt ctccaactga
    22141 ggttgtcgaa tcactaggat tactggacac ttccccagta ttttcgctag atgtatctgg
    22201 tgatactttc tctgaattcg ttgttgattc ttctgcaggt tgaactggat tttctgcttc
    22261 ttgaattgag gttccttctg tagtaccttc atttggattt actggtgttt cttctgttgg
    22321 ttttactgga acttcttcag ttttttctgg accttgttct ttggtcttct caaccggagt
    22381 ttcaggtttt acttgctcaa tattaccctt atattctggc aatggggcta cctgctctgg
    22441 ttcaccttta tcacttacca cagtatctgg cgactctggt tgaacctcag tctcaccttt
    22501 gtcggtcaca actgcttcgg gtaatgtcgg ttgaacttct ggttcgcctt tatcacttac
    22561 tacagcttcg ggcaactcag gctgaattgc gggttcaaca atagatccag actgtacgtc
    22621 cttatgttct acaccagtct caggttgttc ctttataact tgagtttttt tagtaccttt
    22681 ttcgactatt cttggactag gcgcagtcgt tgaagttgaa acaatttctc gcgaaacttc
    22741 ttccttgttt acagagaata ttctgacgat ttcaactttc ttacctaatt taccttcttg
    22801 ttttactctt acagttcctt cagctaaatc aggattttct tgaatttctt cttgaaaatc
    22861 tatttttgtc tctatagttt cctcacgata taagagttca ggtttgttca attgacctga
    22921 taaaacttca tcctgtggat ttaatgtatt taccccagtc ttttcttttg gagaaatctt
    22981 ctcctctttc ttcgtttcta gattcttatg ttcggctaat tgttcttgag aatctgaaga
    23041 ttgtttctct tcttttcttg gattgattaa ttcagtagag aaaggttttt caactacttg
    23101 aacttctgtc ggcttagttg aagaaacagg tgtttgttcc tgaatagctt gtactgttga
    23161 tggatggtct acaaaatttg gtgtaacatt ataatccacc ttttgttgtt ttgtaggagt
    23221 ggctgctgat ttttcttgat tacttacttc aaaatcagaa gtcgtttttc cctctttgat
    23281 atatccaata taagtgtaac ctgaaatctc tttaggaaga ggtaattttt ctccagaggt
    23341 caattcatag tccgtattgt aatttagcaa aagatgattt tctaaagcat ggactgaaac
    23401 taagacacca tttcccattc ccgcaaccaa tactaaatgt aataccgttt tattcttaac
    23461 ctttttcttg gaaacagcaa aaattaaaat tcccagagca gctaagctag caccagcaac
    23521 tagggcttgc ctctcattct tgcttccagt atttggtaat tccgccagtt gattttgaga
    23581 atttaactta taaacaagat aataagtttc atcatcattc tccacgtatg tcggaatatc
    23641 atagacaagc tgcttctttt cttctgatga tagctctgaa tctgccacat atttatagtg
    23701 aactcccgca gtttcttgag catccacaga tgaactagct aatacagaca taaaaaataa
    23761 acttgaaatc gttgcagata caagtcctac tgataatttt ctaaatgaaa aacgctcttg
    23821 tttttcacca aaatactttt ccattattcc tccttgaaat aaaatttata tatgttacaa
    23881 agacctttat tatattagtg tattatctat tatctataga aaaggcagta taccttaatt
    23941 atactcttaa tttacaaaaa agtcttaaaa ttgagatgcg ctttcatact ttgttttata
    24001 ttatttgggg gtacaataac acctaccatg aaatttacac ggtaggtgtt actcatatca
    24061 ctaatcgttc taaaaatggt ttgaggcagt tgaggagaat tccttctatc cagcttcctt
    24121 gtgctgatga gcgatggtct tcctgcaggc ttttttttag aaaatctcgg acttgttctg
    24181 gtgcgatttc aaattcaaag gctttcattt tatagaaaaa gtcgatgaga tgatctgaca
    24241 ggtattcagt tgaaaagggt acttcaccac tttttcgata ttctaataag agtctagaaa
    24301 atcgagcttt ttcttcagga agctcacgaa aataggaatt gaggatccaa gtctgcttct
    24361 gttttctttc aattggatcc tgactggcaa ttcgttggtc tttttccagc tctttttggt
    24421 attgtttggc cttgatagct cgttctgctc tatttttacc aaaaagaatt ttttcccact
    24481 tgcgttcttc ttgagtcagg gtctctgtaa agccaaagta atcttgataa gcacgctctg
    24541 cgggtcccat ggctagaacc agattgtctg catattgctt ggcgatttta tccctcttct
    24601 tgcgttcttt ctctgcctgg atacggagtt cttgttcgta gtcaattttc tccttgccta
    24661 gcttgacaag gtagagttgg tcatccgatt tcccaagtaa aaagggtttg atacactttt
    24721 caaggacttc ttccatccga gcctttttct ttggttccgc cttggtccaa cttcctccct
    24781 gaaagacttc taggaaaagc tggtagtctc tctcaggcgc aaattgattg ccacgattgg
    24841 gtttgaaaac acctttttcc cagagccatt ttagaagtcg ctcgtcaaag tcacttttat
    24901 tgaccttgat tttttccttt ttctgagctt ttctggttag attttcaacc tttctgagca
    24961 gtttttcttc ctcttccaat tgctggtcaa ggggcaatcg atgaaaatga cgaacacagt
    25021 cgctaccaat tggaaagagg cgttggcctg tgacaccgtt aaagagttca taagcgtatt
    25081 tgatggcatt tcgacagaca caattgctac ggccaatacc gttaaaaatc aaggaaactt
    25141 cattccattc cttggtagct tgttcccaag tatccgcttt cgaagcctgt aaaactgcat
    25201 cgtgcaggga ttttctaact ggaagtgtca tgaggtctcc tttctaatac tcaataaaaa
    25261 tcaaagagca aactagaaag ctagccgcag tcagctcaaa acactgtttt gaggttgtag
    25321 atagaactga cgaagtcagt aaccatatat acagcaaggc gaagctgacg tggtttgaag
    25381 agattttcga agagtataag ttatactttt acaacttgaa cctcgtcttt accgagtaaa
    25441 atcaagtatt tttcaatatt ttcaatcgaa taggctcgtg ataaagcctc ttcgtataga
    25501 gctaactgac cacgatagcg gtctacgagt tgacttggtt catcatagcg gtctgtcttg
    25561 tagtcgaaca gaacaatttt gttttcgtaa agcagatagc catcaaggat accacggaca
    25621 acaaagtctt cctgactctt ttggtctcgt ttgagcatgg agaaaggttg ctcgcgatag
    25681 agatggtcgg tattagcaag aatttcctga ccgagtgctg tgtcaaagaa agcaagaatt
    25741 ttatcaagat tgatcttgtc tctgacagct tggctagttt gaacttgttt gagtgtttct
    25801 gttaagctag caagggttag ttgctggctg aggtcaatta tctgcatgag ttcgtgagta
    25861 gcactaccaa tctcagctcc agttaccttt tctttggttg aaaaatctgg caaatcgaag
    25921 ctgattttct tgcctactga ctgaccttga ccagcaatct cgacaccttc catatccata
    25981 actggttcgt agaatttctt gatttgactt ggggtttgaa cactaggaag ttcaatagct
    26041 gcgcggtgaa gagtattata aacttccacc tccttcagca tttccagagc ttctttgatg
    26101 gtatctgact gacgattgtc tgcttgggag ctatcttgga gaggactctt ggtttccaac
    26161 tctccgatag cttctctggt caactgatct tcgtcaataa aacgataact aaagttgagc
    26221 ttgtccttag taaacacttt actgatagcc caaagccaat cttggaaatt ccgtgcttgc
    26281 agtctagtat tgctatttag tttcccattt ttggctgctg ggtattcctt ggattccagc
    26341 ttttcacgag aacccttgcc gacaagatag agctttttct cagcccgcgt catagcaaca
    26401 tacagcaaac gcatctgctc agaatagctt gctagctgta attcctcttc gttctgccta
    26461 taggtcagac taggaatgga gagtttgatg gttttaggat agtggtcttc tactgcccct
    26521 gtctccatct tggcaatata tttgacacca agaccattct gacgactgag aatgacttct
    26581 gacatagagt cttgcttgtt gaaatcttga tccatattga ggataaagac gtaaggaaac
    26641 tccagccctt tactcttgtg gatggtcatg agctctactg catcttttgg cggtgcgacg
    26701 gccacgcttg ccaaatcgtg ctgggcttct aagacttggt caatcatacg aataaaacgc
    26761 gacaaacctt tgaaattgct cttttcaaat tgatcagcac gcagtgctag ggcatagaga
    26821 ttggcctgcc tagcaggacc attcggcaaa gccccaacat agtcataata aaaacggtcg
    26881 ttgtaaatct tccaaatcaa gtcatagaga gagtgggttt tggcatacaa gcgccaagaa
    26941 gctaggatat ccatgaattg ctttagtttt tcaactagag ctgtgtgaat caagcctttt
    27001 tgactacttg ccattttttg tgcattgacc agtttctcat agagattttc gtggacttta
    27061 tcctctgctt tctgaaggga caaacgtgct agctcatcct catcaaaacc aaacattgga
    27121 gacttcataa gggcaaccaa ggcgtagtct tgcaggggat tgtgaatgac acgaagagtg
    27181 tctagcatga cttgcacttc tagggattgg agataattgt tttgctctcc gtcagttttg
    27241 acaggaatcc cgtactcaga cagggcgagg agaatctggt cattacgact gcggctggag
    27301 gtcagaaggg caatttcctt aaaggcaaca cctttttctt gatgaagttt cagaatctcc
    27361 ttgataacta agcgcatttc gcctgttagt ttcgtttctg tttgactctc ttcttcctca
    27421 cctgtatcgt ccttgtcgta gaggagaaat tctgccttgt tgtctggatt gggagtcagt
    27481 ttggtattgg caaaaacaag ctggtgcttg ttatcatagt tgatttcacc gacctcttgg
    27541 tccatgagac gttcaaagac atcattggtt gctgacagca cttctgaact actacggaaa
    27601 ttttccttga ggagaatgag cttgccttct tggggatttt gcgcatagcg ttggaatttc
    27661 tcattgaaaa tctgcgggtc tgcctgacgg aaacgataga tggattgctt gatatctccc
    27721 accataaagc gattgtggcc attagacaac aattccagca tccgttcttg aatatggttg
    27781 gtatcctgat actcatcgac catgacttca tggaagcgct cctgataaga ctcacgaact
    27841 tgtgggaaat tctctaaaat ctcaatggtg taatggctga tatcagcgaa ttcaaaggca
    27901 ttttcctggc gtttgcgctg acgataagcc tccacaaaat gactcataaa ggtttggaag
    27961 gttttagcta gtttccatgt gtcttgatga taacgttctt gatagtcgag aatggttatc
    28021 tggtctgata attgtcctag tttagcaaac tgggtctttc tctcttcgtt gtaggcatca
    28081 gccaggggct tcaaatcagc cttacgactg gcattagtca gagctcgacc gtttttctcc
    28141 ttagagatgg cgacaacacg cgcaagcact gcctgataag cctgactatc ggactcctga
    28201 tttagggagc aaatttcatc cagaattaac tgaacatttt ctaaataggc agcctttgca
    28261 aactccttgg catcgttatc cagatggtaa cggaaaaagc tttccaaatc ccaaagggct
    28321 tgtttgattt gctcagtcag tttttctttt tcactggtaa aatcagcttt ctcaaatcct
    28381 ttgaggaaag attcactcag ccacttttga ggattactgg tggattggag gaagtcatag
    28441 attttataga cctgctggcg cagaccccgt tcgtccttgc cacgcccagc aaagtttttc
    28501 agcaaatgac taaaggtctc tttctgttta ccttggtaat gcgcttcaaa gacctcatga
    28561 aagacttcgt tttcgagaag aagttgctcg ctttggtttt gtaaaatacg gaaattaggt
    28621 gcaatatcaa gcagataacc atgtttgcca aggaattttt gtgtgaaaga gtccatggtt
    28681 ccgatggcag cgtttggtag gtctgccaac tggcgtccca agtgttgttt gaggtcgaca
    28741 tcatctgttt cttggatttt cttgctgatt tttttctcta aacgttcttt aagttcagtt
    28801 gcagccttga cggtaaaggt tgagataaag agttgagaaa tttcgacacc acgcgccaat
    28861 tggtccagaa tgcgctctgc catgacaaag gtctttccag aaccagccga tgctgagacc
    28921 aggatattct gaccagaagt gtagatagct tcgatttgct cggcagtttt cttttgttcc
    28981 ttgctcgaat ttgcttctgc ttcttgcagt ttttgaatct cctcctcact taaaaaggga
    29041 ataagcttca tcgattcaac tcctctctta ttttttcaag ccaagcttgc ttgagttttt
    29101 ctccgaccag acgcttgcca tcagctaggt ccaacttttc taggaaacgg gcttggccca
    29161 gatggtaatt ggcttcaaag cctgtaatag cctgatgttg ctggacgtat ggggcaatgc
    29221 ttctgccatt ttcagtataa ggattgatgg cgaaccggcc tgctaaaatc ttctcagcag
    29281 ctttcttgta aagataggca ttgtagtcca gtaggagctg aaattcctca tctgtcagtt
    29341 gattcgcctt gtttttgtta taaaattcgc ctaaataact gctttctttt tccaagaaga
    29401 gcccttggta tttcatagat ttgctggctt ctaccactgc tcctgccaga ctttttaccg
    29461 ccatcagaga ttggacaggt tcagccattt ccaagtacat ggcgccgaaa aagttctgct
    29521 ccccttctct ttttagggca gcaagatagg ttggtaactg agaattgagc ccattaaaga
    29581 aatgaggaaa ctggaactga gtcagactgg atttgtagtc tactactcct atcgctccat
    29641 tagctttcaa acggtcaatc cggtccacct tgcctcgtac aaagacactg cgtccattgt
    29701 ctaattgaat aaaggcttgg tcttttccac caaaatttgc ttcttctttg attgtttcga
    29761 tggctggatt gtgtcggaga atatgtccag ttgtccgtgc aacatcaagc aaaacttcct
    29821 tggtaaactg ggcttccaaa ctttcttgat aaatagcttc aaattcgcgt tcttgactgg
    29881 tttcttgaat agcttgttct agacgttggt caaaggaatc ttcattaggc aactgcaagg
    29941 cgcgttcaaa gatacgatgc aagaaattcc cgtgactacg ggcatcagga cgcaaacgta
    30001 attcctcctg caagcctaaa acgtagcgta ggaaataact gtattcattg cgataaaact
    30061 ctgtcaaccc cgacgtagac aggtaaaact cctgtttggc aggatagaga gcttgcaagg
    30121 tgtccttggc taaggtcttg ctgcttggac tgattgggat ggctggattt tccagacctt
    30181 gctgatctag ttttttacct atgacacgcg acagaacctt gacaaaagtc aaatcttgct
    30241 cagtatcgct catctcaccc tgctggtgat aggcaaccag actagacaaa agactgtgat
    30301 aggaccccat atcctcctta gacagtcctt tgtgattcat cctcttctct ctccgcctaa
    30361 atccaaaatg gatcaactct tgaagatagg cagattcctt actttcactt tcgttaaaaa
    30421 ggcttggagc cgacaagaac aactgcttac gagcagaatt gaccaaggaa agcatagtgt
    30481 agcgattttt cttgagattt tcactgctgg caatcagtaa ttgaacgcct tcttcggtcg
    30541 tttggtttag gttttgcctt tcttcatctg tcagaagact ggtgttttga gaaatttttg
    30601 gtaaattgtc ctgagttagt ccaatagcat agacaaagtc agcagtcaat ggtgcaatca
    30661 aatcgtaact ctgcaccaga acagtgtcca ctgttgctgg aatggtacgg tattgggaca
    30721 aactcattcc agaatggagc aaggctagga agtcttccag actaacctgt gaaccagcaa
    30781 aaacagtcgc aaattgttct aaaacatggc agaaagcctt ccaaacttcg gcttgtcttt
    30841 cctgttctac agcttccaaa gtggttgtca aatcttgtaa ctgcttggtc acagctcctt
    30901 cttttagaaa gacactccat ttttgtagga ggttttcagc cttttgtttt cggctggcaa
    30961 agagggtttc aagaggtgct aaaattctca ggcggaggac attcaaacgc tcaagattaa
    31021 attttccatg gtgggatttg gtgaaagttt gctgaaaggc tggcaagcca ttgataccaa
    31081 gatagcggat atattgctca aaagcatcaa tatcagactg actgaggtca gtatacaaat
    31141 cagttctaag aagattaatc aaatcctcct gacgaaaacg gtaacgtttt aaagctaaaa
    31201 tagactcgac aaactgagtc aagggatgat gagccatggc ttcgcttcta ccaagataaa
    31261 aaggaatctg atactggtca aaaatggttt tgagagataa ctggtaagaa gctacatccc
    31321 ccaagagaat acgaaaatgc ttgtagctca ggtctgagtt ctcatgtaat ttctgacgaa
    31381 tgctacgggc tactagctcc aactcctcct tttgcgtcaa acaagaccag atttgtaaat
    31441 tttcacggtc tttctcatcg acatccaaag cgagttctga aaagtcataa gaagactcca
    31501 acaaacgaga ggccttgtca aaactatcca tcttctcatg agtttgagaa cagtcctgag
    31561 caggcgtttg gtatttagaa gccagatgat ggagaaattt tacgctggct tggtagagat
    31621 tgccctcgct aaaaggactg gtataggctt tcttactagc ataagccccg ataacaatct
    31681 caacaccttt gccgtgaagt aagtccacaa cccgctcttc ctcagcagaa aaacgagtaa
    31741 agccgtcaat gaccaaggtg atttgattaa aatcactact taccttgtca ttctcaatag
    31801 cctcaatcaa atgggacaac tgacttccct gggctaactg accttgatta agataggctg
    31861 ttactttctc aaaaatcaag agtaaatccg ccctcttatc ctcatctgtt aaattctcca
    31921 agtccaaaaa actcatctga gatttggtca tctcatggta aagctcaatt aactgctgga
    31981 tcaattgagg atcctgctta atagcgccat aaacacgcaa gtccttggga tcgagttcgg
    32041 caaggcattt gtaaaaggcc aacccaagac cgatatcatc aagagtagtt ttaactggta
    32101 aatcattcaa gaccagataa cgagccattt gagcaaagcg cgtgacggta atcgaaaaag
    32161 aagcctgctg ggacaagcat tccagcacgg cgcgttcctt ttcaaaagaa agagagttgg
    32221 gggcaatgta gaagacccgc ttgccagctg caactagctc ttctgcctct cttgttagaa
    32281 tttctgtcaa agaagttcga atatcagtat aaagtaattt catctcagcc tcgttggaat
    32341 ttttcatcac cctatattat accatgatta gcctcgtaaa tctgttaaaa tatttaggcc
    32401 atcctttctt ttcttcatca tctgctaaat cttaaatact tagctttact tgtattagat
    32461 agaataagtc tggctactga aaatcacata ataaaaaagc ctcggtaaca aggctttgag
    32521 ttttatgatt gtttcttagg tacggaatac acttcaatgt gttgtcccag tatcttaatg
    32581 tcgactggta gattgtctga tttatcgcca tcaacatcgg actctaattc gatatcagaa
    32641 gaagttttaa tattacgtgc ctttatatat tcaatattct tgatagaatg attgaactat
    32701 agtaaattga aactataata gtacaccgtg gatgctaaaa tatttctaga aattaatttg
    32761 atttccctaa tcaagctgtt cgtatcttat ttcaatcta
//
LOCUS       DAVBCW010000011        31176 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVBCW010000011 DAVBCW010000000
VERSION     DAVBCW010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31176
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.011"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(322..1170)
                     /gene="dprA"
                     /locus_tag="VQ514_000445"
     CDS             complement(322..1170)
                     /gene="dprA"
                     /locus_tag="VQ514_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HET2499259.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            complement(1268..1957)
                     /locus_tag="VQ514_000446"
     CDS             complement(1268..1957)
                     /locus_tag="VQ514_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HET2499260.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQNIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     HKLEEILKNEN"
     gene            complement(1969..2847)
                     /locus_tag="VQ514_000447"
     CDS             complement(1969..2847)
                     /locus_tag="VQ514_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET2499261.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAAAHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            complement(2837..3706)
                     /gene="licA"
                     /locus_tag="VQ514_000448"
     CDS             complement(2837..3706)
                     /gene="licA"
                     /locus_tag="VQ514_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HET2499262.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRA
                     VKGLASYGGSDEK"
     gene            complement(3723..4745)
                     /locus_tag="VQ514_000449"
     CDS             complement(3723..4745)
                     /locus_tag="VQ514_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET2499263.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPEAEIVVIGRHWEKLELFSFAKECYITDNI
                     PEELAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFKNAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            complement(4750..5457)
                     /locus_tag="VQ514_000450"
     CDS             complement(4750..5457)
                     /locus_tag="VQ514_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HET2499264.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKKIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDSIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            5793..7280
                     /gene="tacF"
                     /locus_tag="VQ514_000451"
     CDS             5793..7280
                     /gene="tacF"
                     /locus_tag="VQ514_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HET2499265.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGDILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVAPEYYLLTTML
                     SEASLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            7290..8093
                     /locus_tag="VQ514_000452"
     CDS             7290..8093
                     /locus_tag="VQ514_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HET2499266.1"
                     /translation="MKQLTVEDAKQIELEILNYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPMDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRLVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSATFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            8095..8904
                     /locus_tag="VQ514_000453"
     CDS             8095..8904
                     /locus_tag="VQ514_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HET2499267.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDQFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNIPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
     gene            complement(9179..12355)
                     /gene="carB"
                     /locus_tag="VQ514_000454"
     CDS             complement(9179..12355)
                     /gene="carB"
                     /locus_tag="VQ514_000454"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HET2499268.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDQVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
     gene            complement(12667..13746)
                     /gene="carA"
                     /locus_tag="VQ514_000455"
     CDS             complement(12667..13746)
                     /gene="carA"
                     /locus_tag="VQ514_000455"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HET2499269.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            complement(13796..14719)
                     /locus_tag="VQ514_000456"
     CDS             complement(13796..14719)
                     /locus_tag="VQ514_000456"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HET2499270.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGAELFFAGPEEWRSQEFADYGKFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            complement(14738..15259)
                     /gene="pyrR"
                     /locus_tag="VQ514_000457"
     CDS             complement(14738..15259)
                     /gene="pyrR"
                     /locus_tag="VQ514_000457"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HET2499271.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            complement(15470..16099)
                     /gene="nth"
                     /locus_tag="VQ514_000458"
     CDS             complement(15470..16099)
                     /gene="nth"
                     /locus_tag="VQ514_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HET2499272.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            complement(16099..16641)
                     /locus_tag="VQ514_000459"
     CDS             complement(16099..16641)
                     /locus_tag="VQ514_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HET2499273.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNQLDQEVLDDD
                     LVLPIENGELDLAESVSDNILLNIPIKVLTAEEEAGQGFISGNDWQIMTEEEYQAQQA
                     VKKEENSPFAGLQGLFDGDE"
     gene            16820..17137
                     /locus_tag="VQ514_000460"
     CDS             16820..17137
                     /locus_tag="VQ514_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HET2499274.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQGTADDIRLQELLNTTMQELKKT
                     EKLNNSILIDLEKFYQPTSPLIGLGSLKLNDQARTAWRNYDKIHYEHVKHVLSLYGPV
                     FGF"
     gene            17376..18935
                     /locus_tag="VQ514_000461"
     CDS             17376..18935
                     /locus_tag="VQ514_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET2499275.1"
                     /translation="MHYRHSRKGNSMIKINHLTITQNKDLRDLVSDLSMTIQDGEKVA
                     IIGEEGNGKSTLLKTLMGEDLSDFTIKGDIQSDYQSLAYIPQKVPEDLKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKTAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLAQELQLTVRGQEKIGIIGPNGVGKSTLLAKLQQLLNDKREISLGFMPQDYHK
                     KLQLDLSSIAYISKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(18979..19878)
                     /gene="htpX"
                     /locus_tag="VQ514_000462"
     CDS             complement(18979..19878)
                     /gene="htpX"
                     /locus_tag="VQ514_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HET2499276.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(19880..20440)
                     /locus_tag="VQ514_000463"
     CDS             complement(19880..20440)
                     /locus_tag="VQ514_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET2499277.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTSEEEKSVPKVDFSGLGD"
     gene            20534..21247
                     /gene="rsmG"
                     /locus_tag="VQ514_000464"
     CDS             20534..21247
                     /gene="rsmG"
                     /locus_tag="VQ514_000464"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HET2499278.1"
                     /translation="MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            21501..22784
                     /locus_tag="VQ514_000465"
     CDS             21501..22784
                     /locus_tag="VQ514_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HET2499279.1"
                     /translation="MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLKANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLLGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(22979..24550)
                     /gene="ffh"
                     /locus_tag="VQ514_000466"
     CDS             complement(22979..24550)
                     /gene="ffh"
                     /locus_tag="VQ514_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HET2499280.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(24562..24894)
                     /locus_tag="VQ514_000467"
     CDS             complement(24562..24894)
                     /locus_tag="VQ514_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HET2499281.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDDFLQEQIEI
                     LTSIDNRE"
     gene            complement(24985..25362)
                     /locus_tag="VQ514_000468"
     CDS             complement(24985..25362)
                     /locus_tag="VQ514_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HET2499282.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELMMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTSHYQVDRQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            25434..26738
                     /locus_tag="VQ514_000469"
     CDS             25434..26738
                     /locus_tag="VQ514_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET2499283.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTVTAALLHDLGHGAYS
                     HTFENLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPVENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKNFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSQRFVNRKVFKSITFSQEDQDQLTSMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESQNPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            26751..27557
                     /gene="yidA"
                     /locus_tag="VQ514_000470"
     CDS             26751..27557
                     /gene="yidA"
                     /locus_tag="VQ514_000470"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HET2499284.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGKYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYERYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRPMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            27600..28598
                     /locus_tag="VQ514_000471"
     CDS             27600..28598
                     /locus_tag="VQ514_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAP domain-containing protein"
                     /protein_id="HET2499285.1"
                     /translation="MNFFSKLFNLKQNNHNRDTNSDCNNFYLNELECGLTPGQLILID
                     WTQKTGRNYNFPRYFKYSLQIDPESTHNQLYKLGYFTKNKTLSYLTVVELKTILSKHN
                     LATSGKKAELITRIINNVNIDNLDIPFEFKLTKEAQNLIIEHSDYIKAYYDKDISMED
                     YCKEKNNISFKATFGDIKWSLLNKQAHRNTVSGDFGCLSNTRKAQGRHLEQEGNIKHA
                     LIYYIESLIITISGLENNFSATDYPVYYPDSIPDYSLKHIQTLMESLSDDDYDFAFDE
                     ALFRFSILNANHFLSKEDIDYLRVNLPRSTAEEINNYLKKYECYSPLNNLELDDFE"
     gene            complement(28765..28836)
                     /locus_tag="VQ514_000472"
     tRNA            complement(28765..28836)
                     /locus_tag="VQ514_000472"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(28801..28803),aa:Arg,seq:cct)
     gene            complement(28879..29226)
                     /gene="rplS"
                     /locus_tag="VQ514_000473"
     CDS             complement(28879..29226)
                     /gene="rplS"
                     /locus_tag="VQ514_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HET2499286.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(29345..29674)
                     /gene="crcB"
                     /locus_tag="VQ514_000474"
     CDS             complement(29345..29674)
                     /gene="crcB"
                     /locus_tag="VQ514_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HET2499287.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            complement(29668..30042)
                     /gene="crcB"
                     /locus_tag="VQ514_000475"
     CDS             complement(29668..30042)
                     /gene="crcB"
                     /locus_tag="VQ514_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HET2499288.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIFLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYFSLV
                     LYLLLSIGGGLLLAYYLGRKKW"
     gene            complement(30039..30305)
                     /locus_tag="VQ514_000476"
     CDS             complement(30039..30305)
                     /locus_tag="VQ514_000476"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HET2499289.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREEVIFEKVRSRVEDKRYQETIVATFSYILKRSRDYQDQNIK"
     gene            complement(30421..30864)
                     /locus_tag="VQ514_000477"
     CDS             complement(30421..30864)
                     /locus_tag="VQ514_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HET2499290.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELAAKVG"
     gene            30968..>31176
                     /locus_tag="VQ514_000478"
     CDS             30968..>31176
                     /locus_tag="VQ514_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HET2499291.1"
                     /translation="MEICQQILEKIKEYNTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVE"
BASE COUNT         9472 a         6708 c         5666 g         9330 t
ORIGIN      
        1 tttcccttat actcaatgaa aatcaaagcg caaactagga agctagccac aggttgctca
       61 aagcactgct ttgaggttgt agataagact gacgaagtca gctcaaaata ctgttttgag
      121 gttgcagatg gaagctgacg tagtttgaag agattttcga agagtattat atgagaaaaa
      181 tcctctactc tactaatcca cttagaaaaa acgcatatta tcaagtgtta aaaacttgct
      241 aatatgcgtt ttattatgga aatgttgggt tcattttctc ttaaaattga ttttttctta
      301 gaattctagc ttaggtcatt tttaaaattc aaattccgca agaacatctt gcccactggt
      361 gaccaatttt gctccttctt gaatcaaatg atggcaaccg tctgatagtc catctaaaat
      421 gctaccagga atagcaaaga catcgcgtcc ttcttccatt gctcgctcac acgtaatgag
      481 actacctgaa cgcatcttag cctctgctac aatcacacca cgacaaagtc cagcaatgat
      541 gcgattacgg gcaggaaaat gaaatttcag aggttgttcg ccaggtccat attcacttag
      601 aaccagatgg tcattgccga tgtagtcttg caagcgttta ttggctttag gataaaacac
      661 atccagtcct gttccaatca ctgcaatggt ttttccgcca ttctgaagag ctgccatatg
      721 agctgctgtg tcaatgccct tggccagacc actgacaata accagttcat tttccaagcc
      781 ttgaatgact ttttcaactg atttagctcc ctgtttgcta caagcacgac tgcccacgac
      841 cgctaccttc gggaatttca ggaggtcaag atttcccttg taaaataaaa gtacaggcgc
      901 atcatatatt tcactcaaat cccaaggata acagtcatct aaaatagaga aagatggaaa
      961 tttttgaaac tctttcgaca aatgcgcatc gtctatctga aaataacgtt ccataaaaac
     1021 ggctggatta cggcaacctg atatatctgc aatatcaccc aacaaaagct cctgatcaac
     1081 attttcaccg tattctagaa ctttcaaaat ctgttgattg gtcaaacctg atttttttaa
     1141 cttatagatt tcatagtttg tgattttcat aaataactcc atttcttttt ctactcatct
     1201 atctattcgt aaaagttcgt aaaaaatcaa aaaaacatcc gactattttg tcagatgttg
     1261 gaagctttta attttcgttt ttaagaattt cttctaattt atgatagtct tggacactat
     1321 caatctcata aatgctattg ccttctaatt cttcaacata gacatctagc tctttgatgt
     1381 tatccttaac catattgtcc caatagagat caacaaattc gccgcttgca taagccttat
     1441 cgataaagct aacaattttt tctgctgttg gagcatccca gaaggataca ccactaagga
     1501 tgcgacctgc cttgctatca acaataatgt tttgaacctt gtagtcatct ccatagacca
     1561 agaaccattc gttggtacaa tcttcacgat agacactaaa ataagtagaa cgtgtcaaat
     1621 cattgcggaa catattttta aagagatagt tatcggcatc gataacataa ctattggcta
     1681 attcttcttt tacaagatag agagagtaaa agttattgta gtcagcgtat ttatcattga
     1741 aaacgagacg aacaccgtat ttctctttca agtaatcgaa ttgttcttta agataaccaa
     1801 caatgatgat gatgtcattg attccttttt ctttgagaaa ctcaatttgg tactcaatca
     1861 aaggtttttg attaacctga accaaggctt taggggtatt ttcagtcata ggacgcaagc
     1921 gagttcccaa tcccgctgct aagatgatgg ctttcacacg aatctccttt attctttaat
     1981 aataatataa actccagcaa tgacgacaag tgacgtcata atggtcagca tatctagcgg
     2041 tgcacccaag aaaacaactg caaacaagac cgtccatact acatagctca cgttcaagcc
     2101 tgtagccttg gctggttgca agcgattgat agcgatataa taagccaagt aggaaatcat
     2161 atcaaaggct gcaaaaacaa tcatgagacc tagcaattgt ccattggcta ctgcagtaaa
     2221 tgactgatga gagaagagca caatcacaag ataggacaag aacgaagtta cttgacggat
     2281 taagagggct tcgatttcac tcaattcact ttccatggca aaagagctaa gaacactctc
     2341 acttccccat gcaatagcac aaaccaaagc acaaagaatc ccaatgtaga aagaattaac
     2401 ctgttcaact ttataggtct gggcaataat ccctccaata atcaagacaa tcccaaatac
     2461 agtatttttc gaaatcttgt gcttcaaaaa gaagaaagcc aatagaactg aaatcgcagg
     2521 gtaaatagcc gatacagatg aagctaaaga acttccgata tacttaactg cataaagatt
     2581 ggcctgcata ccgatagggc ctgctagcaa ggctccgatg ataacactga cattgcgaat
     2641 atttaagaaa attgagaggc gaactttccc ttcttttacc aagagaaaag ctagtaagat
     2701 aaagatgctc aaaaaatcat gagctgcagc caccacaaag ggtgacaaat ctgtaaaaat
     2761 cgaaaagata taagcactaa ccgttagacc caagccccag aaaatacctg agagaaggcc
     2821 aaaaggaact ccgtttttac ttttcatctg aacctccata agaagccaaa cctttaacag
     2881 ctctttggta acgattcaca ccatagtcac caaaatcttc accttgctct tccttataga
     2941 cagtccatag actccaaata gtatcttgta aaattttata aatagcaatc ttttcatgag
     3001 aaaccggtgt ttggtcactc tcatagtgag ataagaaagt ttcttcctct tgggaagtga
     3061 attcagactc taaaaagagg gcagccaaat cccacattgg atcattcatt gatgaatatt
     3121 cccagtcaat caaataaagt cgtccttgag gtgattcgat aaagttttca ggcaccaaat
     3181 cgatatgaca agattttctg tcaacaccta agtcagccag tcttttctct aaggagaaga
     3241 ctgcatttct aacagattca tagttggcat aaggaatttg ttcttcaatc aaggattcgt
     3301 attttttgat ttcttcaaaa ggagcaaatt ctcctcttaa ttccttagca gacgtatgaa
     3361 tagtttgtaa tattggagca attttgtcga acttggtctt gattgacgtt gaatcaagcg
     3421 taatcgcaga ttcgatatac tcatttactt tgataccagc ttcaatatca aaaagataat
     3481 tttttacatc taagcctaaa tcctttagta gttcaagatt gtacttttca tcttgtcgat
     3541 tgataagctt ttctgtccct ttaccaaaga atttaacaat gtattgctta tttgttgttt
     3601 tggccaaata gttttgattg gtcattccac ccagttgttc aacactgagg acttcctctt
     3661 cttgactaag taaggaagaa attttttctt taatgatttt ctccacaatt aacctccagt
     3721 acttatactt cccacttaaa cactgtttta aaggctgtgt ttaaatcggt tgcaaagaca
     3781 cgatgaatat ctttaatttc tcttacaggt tcttctagat aaaggatatt tttaagacga
     3841 ttggcaaatt tcttgacttc catcatttgg atagcatttt taaaatcaat gcgaccagaa
     3901 cgagatgacc caaccaaaat caagcccttt tctaaggcat cgcgagtatt gagattgact
     3961 ttatattcgc taactcccat catgagaatc gttccctgag gacgaatgta gcgaatcaag
     4021 tcattaatag ctggtccagt accatcacca ccacaacatt caaaagcatg gtcaaaggcc
     4081 aactcttcag gaatattatc cgtaatatag cattctttgg caaatgagaa gagttccaac
     4141 ttttcccaat gacgaccaat aaccacaatc tctgcttctg gcaaagtata gttgataata
     4201 ttggcaacca caaaagctaa acttccatct ccaataacgg cgatccgctc ccgcttgcta
     4261 tgagcaagag tcaatagacg attcatagcg tgcatgccca cactgacaaa ctctgtaatg
     4321 gctgcaaccg tatcttcaat agcatcataa gccaccacac gatctttagg gagagaaaca
     4381 aactctctca taaagccatc aaatccacta gacaagaaat gggtccctgt catgtagttt
     4441 tcatagaatt cttcatcact ctgcatagga gactgattgg gaatcatgac aactttttga
     4501 ccaacctcgt aggttccggt cgggtcagaa atgacggttc cacatgactc gtgaatcatt
     4561 gccattggaa gctttttatt caaaatcttg ggatcacgtt ttccctgata gtaacgctga
     4621 tccgcatgac agacagccat gtagttggga cggataagga tatgattctc ttggtcaata
     4681 gcctcttcct gatatttgac attgataaac ttaggcttag ttagttgata aatttgatta
     4741 atcattttac tagtctttct caatcatact ttttgcaatc ttcaaatctg ttacggttgt
     4801 aatcttcaga tttgagtatt cacctttggc caaggccaca tcttttcctt tgatcacaaa
     4861 gattttacat gcatctgtca agatttcctt ctcttcatca gaaagagatc cataaaggtc
     4921 catgaagtcc ttgcaacgga atgtttgagg tgtttgtcct tgataaaggt gagcacgatt
     4981 tggaatatct gtaataaatt gaccattggt actttcaacg atagtatcaa ccgcttctac
     5041 cactgtgtcc actgcgtcat gattttgggc aagttggata ctgtcctgaa tcatgcgaag
     5101 tgtaataaat ggacgaacag aatcgtgggt aacaacgata tcctctggag taagcggacg
     5161 ataagcatca atggcttcaa tgattttctt aatacttgta ttgcggtcag caccaccctt
     5221 tgtaatgatg atacgttcct tataaagagg aagatattta tctacaagat cttctgcatg
     5281 agaaacccag tctccatgaa caccaactac aattttttca atacttggct ccaagacaaa
     5341 tttttcaatt gtatgaatca aaataggtcg atcacctagc tctaaaaatt gttttggcaa
     5401 gttactgatc cccatgcgtg tgccagttcc accggcaaga attcctgcat aaatcatcta
     5461 ttttctcctt tgtcttacta aaaaaactta ttctctctat tataccacaa tctagtccta
     5521 tcataaaaga aatactggtc ctccccttct agaataggag aattatgcag ttctattatt
     5581 caaagaaact agtccatttc tagtaatgac taggaattct gtagcaatta ctaataaatg
     5641 gctgcgaaat tttagagtcc ttcagaataa tatactttcc ttaaagaaaa cttaaaactt
     5701 aagatttgaa acttcaaagt gattacttga cttactgccc ttcatttctt attagctaac
     5761 tttatgatat aatgaaaaac gaggtaaatt gaatgaaaag tataaaatta aatgctctat
     5821 cttacatggg aattcgtgtc ttgaatatta tttttcccat cctaactgga acctatgtcg
     5881 cgcgtgtctt ggaccgaact gactatggtt acttcaactc agtcgacact attttgtcat
     5941 ttttcttgcc ctttgcaact tatggtgtct ataactacgg tttaagggct atcagtaatg
     6001 tcaaggataa caaaaaagat cttaacagaa ccttttctag tcttttttat ttgtgcatcg
     6061 cttgtacgat tttgaccact gctgtctata tcctagccta tcctctcttc tttactgata
     6121 atccaatcgt caaaaaggtc taccttgtta tggggattca actcattgcc cagatttttt
     6181 caatcgaatg ggtcaatgaa gctctggaaa attacagttt tctcttttac aaaactgcct
     6241 tcatccgtat cctgatgctg gtctctattt tcttatttgt taaaaatgaa cacgatattg
     6301 ttgtctatac acttgtgatg agtttatcga cgctgattaa ctacctgatt agttattttt
     6361 ggattaaaag agacatcaaa cttgttaaga ttcacctaag tgattttaaa ccactctttc
     6421 tccctctgac agccatgtta gtctttgcca atgccaatat gctcttcact tttttagatc
     6481 gcctcttcct cgttaaaaca gggattgatg tcaacgttag ttactatacc atagctcagc
     6541 gaattgtgac cgttatagct ggggttgtaa caggtgcaat tggagtgagt gtgcctcgtc
     6601 tcagttacta tctggggaaa ggagacaaag aagcctatgt ttctctggtt aatagaggta
     6661 gtcgaatctt taacttcttt atcattccac tgagttttgg actcatggtt ttaggaccaa
     6721 atgccatcct actttacggt agtgaaaaat atatcggagg cgacatcttg acctctctct
     6781 tcgcttttcg tacgattatc ctggccttag ataccattct tggttcccaa attctcttta
     6841 ccaatggcta tgaaaaacgt atcacagtct atacagtctt tgctgggcta ctcaatttgg
     6901 gcttgaatag tctcctcttt ttcaaccata tcgtggctcc cgaatactac ttactgacaa
     6961 ctatgttatc agaggcttct ctacttgttt tctatatcat tttcatccat agaaaacaac
     7021 tcatccactt gggacatatc tttagctata ctgttcgata ctctctcttt tcactttcct
     7081 ttgtagcaat ttatttcctg attaatttcg tgtatcctgt ggatatggtc attaatttgc
     7141 catttttgat taatactggt ttgattgtct tgctatcagc tatctcttat attagtctac
     7201 ttgtcttcac aaaagatagc attttctatg aatttttaaa ccatgtccta gccttaaaaa
     7261 ataaatttaa aaaatcttag gagtttaaaa tgaaacaact aaccgttgaa gatgccaaac
     7321 aaattgaatt agaaattttg aattatattg atactctctg taaaaagcac aatatcaact
     7381 atattattaa ctacggtact ctgattgggg cggttcgaca tgagggcttt atcccttggg
     7441 acgacgatat tgatttatcc atgcctagag aagactacca acgatttatt aacatttttc
     7501 aaaaggaaaa aagcaagtat aagctcctat ccttagaaac tgataagaac tactttaaca
     7561 actttatcaa gataaccgac agtacgacta aaattattga tactcgaaat acaaaaacct
     7621 atgagtctgg tatctttatc gatattttcc ctatggatcg ctttgatgat cctaaggtca
     7681 ttgatacttg ttataaacta gaaagcttca aactgctgtc tttcagtaaa cataaaaata
     7741 ttgtctataa ggatagcctt ttaaaagatt ggatacgaac agccttttgg ttgctccttc
     7801 gactggtttc tcctcgttat tttgcaaata aaatcgagaa agaaattcaa aaatatagtc
     7861 gtgaaaatgg gcagtatatg gcttttattc cttcaaaatt taaggaaaag gaagtcttcc
     7921 caagtgctac ctttgataaa acaatcgatt taccctttga gaatttaagc cttcctgcac
     7981 ctgaaaaatt tgatactatt ttgacacaat tttatggaga ttatatgacc ctaccaccag
     8041 aagaaaaacg cttctacagt catgaatttc acgcttataa attggaggat taggatgcaa
     8101 tatttagaaa aaaaagaaat taaagaaatt caactagccc tgctggacta tattgatgag
     8161 acttgtaaga aacatgatat tccttatttt ctcagttatg gaaccatgct tggagccatc
     8221 cgccacaaag gtatgattcc ttgggatgat gatattgata tttcccttta tcgtgaggat
     8281 tatgagcgtt tactgaagat tattgaagaa gaaaatcacc ctcgctacaa ggttctttcc
     8341 tacgatacat cttcttggta cttccataat ttcgcatcga ttttggatac ttctactgtt
     8401 atagaagacc atgttaagta caagcgtcat gataccagcc ttttcatcga tgtcttccca
     8461 attgatcaat ttacagactt gagcattgtc gacaagagct ataagtatgt ggcccttcgt
     8521 caactagctt acatcaaaaa atcacgagca gttcacggtg atagcaaact aaaagatttt
     8581 cttagattat gtagctggta cgctctccga tttgtcaatc ctcgctactt ttacaagaaa
     8641 attgatcaac tagtcaaaaa tgctgtaacc aacattcctc aatatgaagg aggagttggg
     8701 atcggtaagg aagggatgaa agaaatcttc ccagttgata cctttaaaga actgatttta
     8761 actgagtttg agggccgtat gttgcctgtt cccaaaaaat atgaccaatt tttaacccag
     8821 atgtatggcg attatatgac accaccatca aaagaaatgc aagagtggta tagtcatagc
     8881 attaaagctt atcgcaaaaa ctgattgagg gggattatac aaactactaa gatagaggtt
     8941 attcaaaaac ataattttag tagaaaatga aatacatatt cccacaataa aacgcatcat
     9001 atcaaggttt ttgaaaaacc ttgatatgat gcgttttata attttaaaga cttttttcta
     9061 tagtagattg aaataagatg cgaacaaatc aattagaaaa ttcaaattaa tttctagaaa
     9121 tattttagta ttcctgtgta ctgttctaaa ttcagtctgc tatatcttat ttttctattt
     9181 aaatcgcttc tgtaacaaag ctacgacttt caagtacctt aagcatggca ttagctgtat
     9241 ctagcgctgt gaagaggggc accccgtgtt caatggctga acgacgaatt tgctcaccat
     9301 cttcgtcagc agttcgtttt gttcctactg tgttaatgat agcttgaatt cttcctttgc
     9361 gtacaaaact tgggatatcc ttatcgtcat caccaatctt accaacaggt tgggcttgca
     9421 agccatggct agcaaagaag gctgctgtcc cttctgtcgc aaggattcca taaccaatgt
     9481 tttggaaacg acgagccaag ttcaaggctt cttctttggc atcatcagcg atggtaaaga
     9541 cgacattacc aaaagttggc aagtgtagat aagaagcttc aaaggcttta tagagagctt
     9601 tttccaaagt agcatcagaa cccataactt cacctgttga cttcatttca ggaccgagca
     9661 agctgtctac cttagctagt ttggtaaagg agaagacagg tgccttgata tgaacacggg
     9721 tgctttcagg gtaaagtcca ttttggtagc caagttctga taaactttga ccaagaatga
     9781 gtttggtcgc tacttgagcc ataggaatat tggttacctt agataggaat ggaacagtac
     9841 ggctggcacg tggattgacc tcaataacgt agactttttc atccttgata acaaactgga
     9901 tgttcatcat tccaaggcag tgaagaccga ttgctaagcg tttggtgtag tctgcgatgg
     9961 tctcctgaac cttttgcgac aaggtttgtg gtgggtaaac agccattgag tcacctgagt
    10021 ggacaccagc acgttcgata tgctccatga taccaggaat gagtacattt ttaccatctg
    10081 aaatggcatc aacttcgcac tcttgcccaa cgatataaga gtcgacaaga actgggtggt
    10141 ctggactagc cttaacagca gttcgcatgt aagaacgaag gtcttcttcg ttttcaacga
    10201 tttccatggc acgtccacca agtacataag atgggcggac aagaactggg aagccaatct
    10261 tgcgagctgc aagagctgct tcttcttcat tggtagctgt ttgtcctggt ggctgtggaa
    10321 tatccaattc tttgagagct tgctcgaaga ggtcacggtc ttcggcacga tctaggtcag
    10381 caacctgtgt accaaggatg gtcacacctg cttttgccaa tggctccgca aggttgatgg
    10441 ctgtttgacc accgaactga acgataactc cctttggttg ttccaagtca atgacgttca
    10501 taacatcttc gaatgtcaat ggctcaaagt aaagcttatc tgatacagag aagtctgttg
    10561 aaacggtctc tgggtttgag ttcatgatga tagcttcata accagctgcc tggatagcct
    10621 taacagagtg aacggttgcg tagtcaaact caaccccttg accaatacgg attggacctg
    10681 aacctaggac aagtacagat tctttatcag atctgataga ttcattttcc caaccatagg
    10741 ttgaatagaa atatggcgtt tcggagtcga actctgccgc acaagtgtct accatcttat
    10801 aaactggaac aatcttgttt tccaagcgaa gttggcgaac ttgatcatca gtcgttcccc
    10861 agagttcagc aatcttacgg tctgaaaaac cattaagttt ggctgttttc aaaacttcta
    10921 gatcttgtgg atgagcaccc aattcttgct caatttcaaa gatatgcaag agtttatcaa
    10981 gatagaagat atcaattttt gtaagctctg caatttcttc aggtgtgtag ccacgacgaa
    11041 ttgcttctga tacgtagaag agacggtcat cttgggcttt gacaaccttt tcaatcaagg
    11101 catcatcaga aactgctgca agttcaggta tttcattgtg gtgcacccca atttcaaggg
    11161 agcggcaggc cttgagaaga gattcctcga tgttacgacc gattgccatg acttctccag
    11221 tcgccttcat ttgtgtaccg agacggcgtt cacccttttc aaacttgtca aatgggaaac
    11281 gtggaatctt agcaactacg tagtcaaggg ctggttcaaa catggcatag gttgaacctg
    11341 taactgggtt tataacctca tccaaggtca aacctactgc aatcttggca gccaacttag
    11401 caatcggata tcctgtcgct ttagaagcaa gggctgacga acgtgataca cgagggttta
    11461 cttcgataac ataatacttg aagctgttag gatcaagagc tagctgaaca ttacatccac
    11521 cttcaatctt gagggcacga ataatgctca agctcgcatc acgaagcatt tggttttcat
    11581 agtctgacat ggtttgcgca ggggcaaata caatggaatc ccccgtgtga atcccaactg
    11641 ggtcaaagtt ttccatgtta caaacaacca aggcattgtc agctgagtca cgcatcactt
    11701 cgtattcaat ttccttgaaa ccggcaatcg aacgctcaat caaacattgg gtaacaggtg
    11761 acaatttcaa accattttca gtgatttcac gcaattcttt ctcgttggca cacataccac
    11821 caccagtacc accgagggta aaggctggac gaacgatgac tgggtagcca attgtcgctg
    11881 caaaggcaac tgcttcttct actgtgttaa caatttcaga ttctggaatg ggttgttcaa
    11941 gctcttccat caattgttta aagaggtcac ggtcctccgc ttggtcaatg gcagataatt
    12001 tggtacccag aagttcaacg ccaagctcat ctaagatacc atttttagac aattccatgg
    12061 ccatgttgag acctgtctga ccaccgagtg ttggtagcaa ggcatctgga cgttccttac
    12121 gaagaatacg tgtcacaaac tcaagtgtaa tcggttcaat gtaaaccttg tcagcaattt
    12181 ccttgtccgt catgatggtt gcaggatttg agttaaccaa aacaacctca taaccttcct
    12241 ctttcaacga caagcaagcc tgagtcccag cgtagtcaaa ctcagcagcc tgaccaataa
    12301 taatcggacc agaaccaatc accataattt tttgaatatc agtacgttta ggcatagttt
    12361 atgatacaaa gcccgtaaag aacacagtga aaataggaaa ctcggcgcag acgccttcag
    12421 cgtcaagacg atgtttatct ttttcacaca gttcttaggg cgtgttcact tccgcgaaca
    12481 atgtatcttc tcctttctat tcgtcgcctc tcagggcgac attaaataag atacaaagga
    12541 cgactagaaa gcgattgaat tttaggaaat caaggaagga ttgacaatcc aagttggttt
    12601 ctctaaattc tgagctttcc gtccatgttc agttacataa attctccgac gagcttttac
    12661 tcattcttag tttgattgtt taaaaacttc catcatctcg ataaactcgt caaataggta
    12721 gctagcgtcg tgtggcccag gagctgcatc tgggtggtat tgaacagaga aagcaggttg
    12781 gtatctgtgg cgcacacctt ccactgactt gtcattgatt tcttcgtggg taataatcaa
    12841 gtgctctggc aaatcctcgc ggctgactgc ataaccatgg ttctggctgg tgaagtctac
    12901 tcgtcctgtt gcgatttcac gtaccgcatg gttgaatcca cggtggccaa acttcatctt
    12961 ataggtctta gccccgtttg ccattgcaaa gagttggtgt cccatacaaa taccaaagat
    13021 tggaattttt ccttgtacac cgcgaatcat gtcgagtgct tgtggaacgt cttctgggtt
    13081 acctggacca tttgacaaca taactccatc aggattgaga tggagaattt cttcagccgt
    13141 tgtcgaataa ggaacaactg tcacgttaca gttgcgttta gaaagttcac gtaggattga
    13201 gtgcttgaga ccaaagtcca ctagcaccac gctcaaacca actcctggag ctggataaga
    13261 cgttttagta gaaacctgtt tgatattgtc tgtcggtaaa actgttgctt ggagctggtc
    13321 cgtcacatgg tccatactgt ccccaacatg ggtcaaggtt gcacgcatag taccatgctt
    13381 acggataatc ttggtaagag cacgcgtatc aattcctgaa atccctggaa ttttcttggc
    13441 tttcaaaaat tcatccaagg tcatttggtt gcgccagttg ctagctctac gcgcttcttc
    13501 aaaaacaacg actcccttac aagttggaat aatggattca taatcatcac gattaatacc
    13561 ataatttcct accaaaggat aagtaaaggt caagatttgt ccattataag actggtctgt
    13621 aatggattct tggtagccgg tcatccctgt attaaagacg atttcgcctg ttacatcaat
    13681 atctgctccg aaggccttgc cttcaaaaac tgtgccatct tctaatacta gaattctttt
    13741 tgtcatattt tcacctctcg tggacgctca ctggcgtctt ttaacgtctt gtgttttagt
    13801 tggcgtttct actcgctagt acggattcta agattgccat tcgaacaaag acaccattgg
    13861 tcatttgttg gacaatccgt gattttggtg cttcaaccaa gtggtctgct atttctacat
    13921 cacgattgat tggagctggg tgcatgagga ttgctgtttc tttcaaacga tcgtaacgtt
    13981 cttgagtcaa gccatgttgg gcatgatagt cttcttttga aaatacagct ccactatcat
    14041 ggcgttcgtg ttgcacacgg agaaacatca tgacatcaac ctgatcaatg atttcatcaa
    14101 tggttacaaa ctttccatag tctgcaaact cttgacttct ccattcctca ggtccagcga
    14161 aaaagagttc agctcccaag cgtttcaaaa tctgcatatt ggatttggca acgcgtgagt
    14221 ggtccaagtc acctgcaata gcaactttaa gaccctcaaa gtggccaaat tcctcataaa
    14281 tggtcatcaa atcaagcaag ctctggctag ggtgttggcc cgaaccatct ccaccattga
    14341 tgatggaagt cgtaatcgtt ggactagcaa tcaattctct atagtagtcg acctctggat
    14401 ggcgaatcac acagacatcc actcctaaag cagacagagt caaaatggtg tcataaagtg
    14461 tctcaccctt attaaccgaa ctagtcttca catcaaagtc aagtcgttcc aatccaagtt
    14521 taatctctgc gacttcaaag gacttatgtg tccgtgtaga atcctcaaag aagagattgg
    14581 aaacaatcgg atggtcttca tagggaagct gggctccatt tttaaactca attcctcgct
    14641 tgatcaattt cattacttga tcgacagtga ggtcttccat ggacaccaca tggttcaatg
    14701 cttgttgatt ttctgacatg gctactcctt taactttcta agcttcttca gtaatcagaa
    14761 ctctgtcttg gtcatcaagt tctgtcatct ctacgatgat ttcttcagaa cgactggttg
    14821 ggatattttt tccaacgtaa tctggacgga ttggcaattc tctatgtcca cgatcgacta
    14881 gaactgctaa actcacacgc gcaggacgac catgaccgac aatattatca atagcagcac
    14941 ggatggtacg acctgtatag agcacatcat ccaccaagat aacttcgcgg tctgtcacat
    15001 cgacagaaac caaagaagta tcttctccac ttttaacatc atcacggaaa ggtttagtat
    15061 ccaattccac aacaggaact gaaagatttt ctaactgctt caaacgttct tggattcggt
    15121 gggcaataaa gacaccacga gttttaatac cagccaagac gatcttattc aaatctttgt
    15181 tgcgttcgat aatctcataa gtaatacgcg taatcgctcg tttgacggtc aattcgtcta
    15241 caacttcttt tgttttcatg acaaacctcc aaaaagaaaa gtctccttaa acaaggagac
    15301 ttgaaattta tagccaagcg agccctactg caaacagtat agacttcacc cttctacttt
    15361 atcgcgctcc ttgcctgcct cacgggacag gtttaaagga atatttagtt atcatttact
    15421 atagcacaaa gcatgcttaa aatcaagcaa aaagtttcaa tgtagcatct tacaaattgc
    15481 taaaatcata taattgtggg tactggtcac actctggatt ttttggatgg caaatggctc
    15541 ttccaaaata aatcatggcc tgatgggcag ctaaccactg ctcaggcggc aagatatcca
    15601 tgacccgctt ttccacctca agtggcgtcg ctgatttttt gacaatatcg tggtgtttgc
    15661 aaatacgctc cacatgagta tccactgcaa aggctggaat cccaaatcct acactcatga
    15721 caacattggc tgtcttgcga ccaacacctg ccaaactctc caattcttca cgtgtctgag
    15781 ggacttgacc atcaaaatcg tctagtaact gttgggcaca ttttttaagg aatttagctt
    15841 tattccgata caatcccaag cgagaaatat gtgaagcaat ctcactctct gtcgctacag
    15901 acatagcttg gggtgttgga aaggcaacaa agagacctgg tgtggcctta tttaccgctg
    15961 catctgtcgt ctgggctgat aacatgaccg caaccaggag ttcaaaatga ttggtaaaat
    16021 caagactagg cttggcatct gggaagaggg caatgatttc ttctagcacc tttcgtgctc
    16081 gtttttttga caagaccatt attcatctcc gtcaaatagt ccttgtaagc cagcaaaagg
    16141 actgttttct tctttcttta ctgcttgttg agcttggtat tcttcctctg tcatgatttg
    16201 ccagtcattt cctgagataa atccttgacc agcttcttct tcagccgtca agaccttgat
    16261 aggaatgttt agcaggatat tgtctgatac actctcagca aggtcaagct ccccattttc
    16321 aatgggcaag accaagtcat catctaaaac ttcttgatct agctggttag ttgcgccttc
    16381 catgaaaact tccgtgactg gataagattc aactaactca actggctcca tactgcgact
    16441 cgaagcaaga acaatggtat aagatagttg ataatctaag aaatacatac ggtcttcata
    16501 ttgtactttc ccaactgcaa ggatatcttt tacatctaaa atttcttgat tacgtgcacg
    16561 caggtcatca actaaatcta acgtttgttc aaagttcaaa ccttcagact gcttacgaat
    16621 ttcttgaata tttaatttca tacttcctcc ataaagattt actctcttga ttataccatg
    16681 aaaaggctac aaatcagcac accaaacttt gtaattaaaa ttcaaaattt taacatattt
    16741 actatgacag ttttattttt tagtgctata ctatagggaa agagtacatc agatcaagga
    16801 ggatgctcat atggaagaca tggaagacaa gaaactcatt caactcctat ccaagttaaa
    16861 taaaagctac caaaactgta aacagggtac ggcagatgat attcgactac aagagctgct
    16921 aaacactact atgcaagagc tcaaaaaaac ggaaaagttg aacaacagta tcttaattga
    16981 tcttgagaaa ttttaccaac ccaccagtcc tctgattgga ctgggtagcc taaaactaaa
    17041 cgatcaagca cgcactgctt ggcgaaacta tgataaaatc cattacgagc atgtcaaaca
    17101 cgtactaagt ctctatggac ctgtttttgg attttagagc atagaatttc cagttttctg
    17161 ttgacaaaat ttccttaaaa gtatagtata aagatactaa tactcggagg taagggagac
    17221 atgaacaact aagtctatca aataaagaac ctttatttag tagatcttgt ttttgtctct
    17281 ttttgtgtgc tcttttatgc tctttttctg gcatgttaat agagtttttt tgacatagac
    17341 tttgggctct actaggtaaa gtagagcttt ttgttatgca ctatcgacat tctagaaagg
    17401 gcaatagtat gataaaaatc aatcacctga ccatcacaca aaacaaagat ttacgagacc
    17461 ttgtctctga cctaagcatg accatacaag acggggaaaa ggtagctatt attggcgaag
    17521 aaggaaatgg taaatcaacc ttacttaaaa ctttaatggg ggaagatttg tctgatttca
    17581 ctatcaaggg agacatccag tctgattatc agtcactggc ctacattcct caaaaagtcc
    17641 ctgaggacct aaaaaagaaa actttacacg actacttctt tttagattct attgatttag
    17701 actacagtat cctctatcgt ttggcggagg aattgcattt tgatagcaat cgtttcgcaa
    17761 gtgaccaaga gattggcaat ctatcagggg gcgaagcttt gaaaattcag cttatccatg
    17821 agttagccaa accctttgag attctatttt tagatgaacc ttcaaatgac ctagaccttg
    17881 agacagttga ttggctaaaa ggccagattc aaaagaccag gcaaaccgtt attttcattt
    17941 cccatgatga agactttctt tctgaaacgg cagacactat tgttcacttg cgactggtca
    18001 aacaccgtaa agaagcggaa acgctagtag agcatttaga ctatgatagc tatagtgagc
    18061 agagaaaggc taattttgcc aaacaaagtc agcaagctgc taacaaccaa agagcctacg
    18121 ataaaaccat ggaaaaacat cggagagtta agcaaaatgt agaaactgcg cttcgagcta
    18181 ccaaagatag tactgccggt cgcctattgg ctaaaaagat gaaaactgtc ctctcacaag
    18241 aaaaacgcta cgaaaagaca gctcagtcca tgactcaaaa gccacttgaa gaggaacaaa
    18301 tccaactttt cttttcagac atccaaccat taccagcttc taaagtctta gtccaactgg
    18361 aaaaagaaaa tttgtccatt gacgaccgtg ttttggctca ggaactgcaa ctaactgtcc
    18421 gtggccaaga aaaaatcggt atcatcggcc caaatggtgt tgggaaatca actctgttag
    18481 ccaaattaca gcaacttctg aatgataaaa gagagatttc acttggtttt atgccacaag
    18541 attaccacaa aaaactgcaa ttggatttat cctcaatagc ttatatcagc aaaactgggg
    18601 aaaaagagga acttcagaaa atccaatctc acctagccag tctcaatttc agttatccag
    18661 aaatgcagca tcaaattcgc tccttatctg gcggacaaca gggaaaactc ctgcttttgg
    18721 atctagttct gcgcaaacca aactttctcc tgctggatga acccacacga aacttttctc
    18781 ccacttctca accccaaatc agaaaactct ttgcaaccta tccaggcggt ctcatcactg
    18841 tttcgcatga ccgtcgtttc ttaaaagaag tctgctcgat catctatcgc atgacagaac
    18901 acggtttgaa gctagttaat ttagaagatt tataaatttg caacatagca aaaatccaga
    18961 gacgacctct ggattctttt acatctgttt taaacgttca atccgttctg agataggtgg
    19021 gtgggtataa aagagttttt ggaacccccc acctttctta ggatcattga tataaagggc
    19081 actgctagca tcatcgacgt ggcgactcat aggtttgcta ttgtccaact tatctagggc
    19141 attaatcatt ccctggggat tgcgagtcag ctcgacacta gatgcatctg ccagaaattc
    19201 cctctgacga gaaatagcga gctgaaccaa ggttgcagcg agaggtgcca gtacaatagc
    19261 tagtagggaa accactagca taatgatttc aagaccattt ccatctcggt catcatcact
    19321 tcgtctgcga cctgctccac cccaccacat catacgacct gccatactag aaagcatggt
    19381 gatagcacta gcaagggcaa ctgcaatagt cgaaatacgg atatcataat tacgaatatg
    19441 actgacttca tgtcccataa cagcttctag ttcttcacga ttcatgatag ctagtagacc
    19501 tgaagtcgca gcaacagccg cattttgagg attagaacct gtcgcaaagg catttaaggc
    19561 tggatcatca atgatgaaaa cacggggcat aggaatctga gcgaccagag ccatatcttc
    19621 cactacatgg tagaggtctg gtgccgtttg ctcatccacc tcacgcgctc cattcatgga
    19681 catgacaatc tctgtcgatt gaaaaatcat agacaaagcg tagataaagc cgataatcag
    19741 tgcgataacc aaaccaccaa gtccagatct tataaagaga taaccaaccg cataaccaac
    19801 aagagctaag agtaggaaaa ataccagcaa caaaatccag gtttttcgtt tattgcttgc
    19861 aatttgatca aacaacatct tagtcaccta aaccgctaaa atcaacttta ggaaccgact
    19921 tttcctcttc agatgtttga aggaaatctg ccgctttaaa tccaaacatt ccagcgataa
    19981 tattgctcgg gaaagtttct aattttacat tgtagttgct gacaacactg ttatagagtt
    20041 gacgagagta agaaatttta ttttctgtgt ttgtcaactc ctcttgcaat ttaacaaagt
    20101 tagcactagc tttcaaatct ggatagcttt ctgcaactgc aaaaatacct gaaacctgac
    20161 gagtgagggc atcactggct ttcatagctt ctgctggtga agtcgctgcc gccacttggt
    20221 tacgtagttc tgccaccttt tcaagggtag aaccttcata tttggcataa ccttttacag
    20281 tctcaatcaa gtttggcaag aggtcatttc gacgtttcaa ctgaacatca atctgactcc
    20341 aagcctcctt ggtttgcata cgatttttaa ccaaaccgtt atagctaaca atcacaaaaa
    20401 taacaataag agcgataact ccaagaataa tccaagtcat aatataagtc ctttctgctt
    20461 ttagattagt accagtatat caaattttct atgattgtgg taaaataaga tgatactaaa
    20521 gaaggaaata actatgaaac caaaaacatt ttacaacttg cttgccgagc agaatcttcc
    20581 actttcggac cagcaaaaag aacaatttga acgttatttt gagctcttgg tcgagtggaa
    20641 tgagaagatt aatttgacgg cgattacgga caaggaagaa gtttatctca aacattttta
    20701 cgattcgatt gcacccattc ttcaaggttt gattcccaat gaaactatca aacttcttga
    20761 tatcggggct ggggcaggat ttcctagtct accaatgaaa attctctatc cggagttaga
    20821 tgtgaccatt attgattcac tcaataagcg catcaacttc ctacaactct tggctcaaga
    20881 actggatttg aacggagttc atttctacca cggacgtgcc gaagattttg cccaagacaa
    20941 gaacttccgt gctcaatatg attttgtaac agctcgtgcg gttgcccgta tgcaggtcct
    21001 atctgaattg accattccct accttaaggt tggtggaaaa ctattggcac tcaaggccag
    21061 caatgcgcct gaggaattat tagaagctaa gaatgccctc aatctccttt ttagtaaggt
    21121 cgaagacaat ctcagctacg ccctaccgaa tagagatccg cgctatatca cagtggtaga
    21181 aaagaaaaaa gaaacaccaa ataaatatcc gcgtaaggct ggtatgccaa ataaacgccc
    21241 actttaaatt ttttagtaaa caaatgttta caaaatcagc ctcgctcttt tatttctagg
    21301 ctcgggaaaa aatgatttac aaaatcattt ttttctgcta tactatccta agcaaaggtt
    21361 tttaatgtca tcccgtgagg tgacgaagac gcagaaatat ttaaaactct ttaaaatcta
    21421 aattttaaag aagtcttact ctgagggcct attgctgtaa aataatgggc tcttttttga
    21481 tgcccaaaag tgaggtttat atgaaacaag aatcaactgt tgatttgtta ctagacgttg
    21541 accaacgtcc ttcggctgga aaaggaattc tccttagttt ccaacacgtt ttcgccatgt
    21601 ttggtgcgac catcttggta ccattgattt tgggaatgcc tgtatctgtt gccctttttg
    21661 cttcaggtgt tggaacactc atctacatga ttgctactgg ttttaaagtt ccagtttatc
    21721 taggttcttc atttgccttt atcacagcta tgtcactggc tatgaaagaa atgggggggg
    21781 atgtatctgc tgcccaaaca ggggttatct tgactggttt ggtctatgtc cttgttgcta
    21841 ccagcatccg atttgtagga acaaaatgga ttgataaact cttgccacca atcattatcg
    21901 gtcctatgat catcgttatc ggtcttggac ttgcaggttc agctgttacc aatgcaggtc
    21961 ttgtagcaga cggaaattgg aaaaatgctc tggtagccgt tgttactttc ctaattgctg
    22021 cctttatcaa tacaaaagga aaaggcttcc tacgaatcat tccattcctc tttgccatta
    22081 tcggtggtta ccttttcgca ctaactcttg gcttggttga ctttacacca gttcttaaag
    22141 ccaactggtt cgaaattcct ggtttctact tgccatttag cacaggtggt gcctttaaag
    22201 agtacaatct ttactttggt ccagaagcca tcgctatctt gccaatcgct atcgtaacaa
    22261 tttctgaaca tatcggagac catactgttt tgggtcaaat ctgtggtcgt caattcttaa
    22321 aagaaccagg tcttcaccgt actcttcttg gtgacggtat cgcaacttct gtttctgcct
    22381 tccttggtgg accagccaat acaacttacg gagaaaatac aggggttatc ggtatgactc
    22441 gtatcgcttc tgtctcagtt atccgtaacg ctgccttcat cgcgattgcc ctcagcttcc
    22501 ttggtaaatt cactgccttg atttcaacta ttccaaacgc tgtacttggt ggtatgtcaa
    22561 tccttctcta tggggttatc gccagcaatg gtttgaaagt cttgattaaa gaacgtgttg
    22621 atttcgctca aatgcgaaac ctcatcatcg caagtgctat gttggttctt ggacttggag
    22681 gagctatcct taaacttggt ccagttacac tttcaggtac tgccctttca gccatgacag
    22741 gaatcatctt gaacttgatc ttgccatacg aaaataaaga ctaagagtct aaatacacct
    22801 aatccactca gacagctgag tggatttttc gtataccata ataaaagtgt cttaacaaaa
    22861 ttattaaaat caaaaaacgt ataatatcag atattctaaa accttgatac tgtacgtttt
    22921 atcatagaaa tttttacttt attttctcat caaatgagat ttgcatcaat ctcttgtctt
    22981 acttgcgttt cttcttcgct ttcttcattt tgttagccat acgtttcatg gactgtttca
    23041 tggcaaattc accaatttta cctttcaaac cgccaccaaa catctggctc atatctggca
    23101 ttcctgctcc tccgagagct gataagtcag gcataccgcc ttgtcccatc attccttcaa
    23161 gggcagacat atccattcct cccatatttg gcatattttt aggaaggtta tttggattaa
    23221 tccccatttg cttcatcatt ttattcatat ccccagacat aacaccctgc atgagctgtt
    23281 tagcctggtt aaagtccttg atgaatttat tgacttcgac gaatgtattt ccagaaccag
    23341 cagcaatacg acggcgacgg cttggattta acaaatctgg gttttcacgc tcttcaggtg
    23401 tcatcgaaga cacaatggca cgtttacgag caatctggcg ttcatccacc ttcatgtttt
    23461 gaagggctgg attgttggcc atacctggaa tcatcttgag caagtcttcc atcggcccca
    23521 tattttgcac ctgatctaat tgatcgatga aatcattaaa atcaaaggtg ttttcgcgca
    23581 tcttctcagc catttcaagg gctttttgtt catcgtattc ctgagaagct ttctcaatca
    23641 aagtgagcat atcccccata ccaagtatac ggctagacat gcggtctggg tggaaggttt
    23701 caatgtccgt aatcttttca cctgtaccag tgaacttgat tggttttcca gtaatgtgac
    23761 gaacagacag agcagcacca ccacgagtat cgccatcaat cttggtaagg atgaccccag
    23821 tcacttccaa ctgagcatta aactcacgcg caacattggc tgcttcctga ccaatcatag
    23881 catcaacgac aagcaagatt tcatttggtt gagccaatgc tttcacatca cgaagctcat
    23941 tcatgaggag ctcatcaatc tgcaaacgac ccgcagtatc aatcaagaca tagtcgttat
    24001 gattagtttg ggcttgctcc aaaccttgac gtacaatctc aacagctggt acttctgttc
    24061 caagtgcaaa gacaggcaca tcaatctgtt gtcccaaggt cttaagctgg tcaatggcag
    24121 ctggacgata aatatccgcc gcaatcatca aaggacgagc attttcttct ttcttgagtt
    24181 tgttggccaa tttaccagca aaggttgttt taccagcccc ttgtaaacca accatcatga
    24241 tgatggttgg aatcttaggt gacttgataa tttctgccgt atcagaacct aaaacggctg
    24301 tcagttcctc atcaacgatt ttaataatct gttgcgcagg attaagtgta tcaatgacct
    24361 catgcccgac tgcacgctca cgaactttct tgataaagtc ctttacaaca ggcaaggcaa
    24421 cgtcggcctc gagcaaggcc aagcgaattt ctttggttgc ctcttggaca tcagattcag
    24481 agatttttcc ttttttacgt agatttttaa agacgttctg caaacgttct gttaaacttt
    24541 caaatgccat ttttcttcct cttattctct attatcaatg cttgttaaaa tttctatctg
    24601 ctcctgcaga aagtcatcct tgggatagcg ctccaaaatc tgatcaaaaa tctgactgcg
    24661 gacaatatag tccgagtaca tgtgcaattt catctcataa tcttccagaa tcttttctgt
    24721 tcgcttgata ttgtcataga cagcctgacg actgacacca aactcctcgg caatttcagc
    24781 aaggctgtaa tcatcagcgt agtagagctc gatataattc atttgcttat ctgtcaaaag
    24841 cgccgcataa aattcaaaga gcgcattcat acgattggtt ttttcgattt ccataacttt
    24901 tattatacca aaaattagcc taatctacca cactaggaag ccgatccaag aagatagata
    24961 gctaaatttg aaaaagacat gagcctagcc ccaagtaatt tccaattgat agctggcaaa
    25021 aggatgtccc tcttgatttt gtagttgata atctagttca atcttttgcc tatcaacttg
    25081 ataatggctc gtttggatga taaactcctg catgcccata ggtgtaggaa tataggctaa
    25141 actatcgcta tcctttagaa agcgcataat ggtcttggga ttagaaaatc ggctcatcat
    25201 aagttcttga ccatgaaatt taatcactac tttttccttt tcctcattat agaaaagcag
    25261 gtagctataa tctccttttt catgcacttc cacatcataa agctggtcaa tcacttccaa
    25321 ctgctcatca aactgaattg tatttcgcat ccgaatcttc acatcaggcc ctctttcttg
    25381 tctcttgtcc tactatttta ccaaaaagag caggattttg ctataatggt catatgaacg
    25441 aaaaagtatt ccgtgaccca gttcacaact acatccatgt caataatcaa atcatctatg
    25501 acttgattaa tacaaaagaa tttcagcgtt tgcgccggat caaacaactg ggaacttcca
    25561 gttatacctt ccacggtgga gaacacagtc gcttctctca ctgtctagga gtctatgaaa
    25621 ttgcacgacg catcacagag attttcgaag aaaaatatcc tgaggaatgg aatcctgccg
    25681 agtctctctt gaccgtgacc gctgctctcc tacacgacct tgggcatggt gcctactccc
    25741 atacttttga aaatctcttt gatacagacc atgaagccat tactcaggag attattcaaa
    25801 atcctgagac agagattcac caagtcctgc tacaagtggc acctgatttc ccagaaaagg
    25861 tggccagtgt cattgaccat acctatccta ataagcaggt cgtgcagctc atttctagtc
    25921 agattgacgc agatcgcatg gactatctct tgcgcgactc ctattttaca ggagcatcct
    25981 atggggaatt tgacctgact cgcatcctcc gagtcattcg tcctgtcgaa aatggtatcg
    26041 cctttcagcg caatggcatg cacgccatcg aagactacgt cctcagtcgc taccagatgt
    26101 acatgcaggt ttatttccac ccagcaacac gcgccatgga agttctccta cagaatcttc
    26161 tcaagcgcgc caaggaactc taccctgagg acaagaattt ctttgcacga acttccccac
    26221 atctcctgcc tttcttcgaa aaaaatgtga ccttgactga ctatctggct ctggatgatg
    26281 gcgtgatgaa tacctacttc cagctttgga tgactagtcc tgacaaaatt cttgcagatt
    26341 tatctcaacg ctttgtcaac cgcaaggtct ttaaatccat taccttttca caagaggacc
    26401 aagatcaact tactagcatg agaaaattgg ttgaggacat cggctttgat cccgactact
    26461 atactgccat tcataagaac tttgacctcc cttatgatat ctatcgtccc gaatctcaaa
    26521 atccacggac acagattgag attttacaaa aaaatggaga actggccgaa ctctctagcc
    26581 tgtctcctat cgtccaatcc cttgctggca gtcgccacgg agataatcgt ttctatttcc
    26641 ctaaagaaat gttagaccaa aacagcatct tcgcaagcat tacccagcaa tttttacact
    26701 tgattgagaa cgatcatttt accccaaata aaaactagaa gaggaatttt atgagtatta
    26761 aactaattgc cgtcgatatt gacggaaccc tagtcaacag ccaaaaggaa atcactcctg
    26821 aagtcttttc tgccatccaa gatgccaaag aagctggtgt caaagtcgtg attgcaactg
    26881 gccgccccat cgcaggtgtt gccaaacttc tggacgactt gcagttgaga gaccaaggtg
    26941 actatgtggt gaccttcaat ggtgcccttg tccaagaaac tgctactggc catgagatta
    27001 tcagcgaatc cttgacctat gaggattatc tggatatgga attcctcagt cgcaagctcg
    27061 gtgtccacat gcatgccatt accaaggacg gtatctatac tgcaaatcgc aatatcggaa
    27121 aatacactgt acacgaatca accctcgtca gcatgcctat cttctaccgt acccctgaag
    27181 aaatggctgg caaagaaatt gttaaatgta tgtttatcga tgaaccagaa attctcgatg
    27241 ctgcgattga aaaaattcca gcagaatttt acgagcgcta ctccatcaac aaatctgctc
    27301 ctttctacct cgaactcctt aaaaagaatg tagacaaggg ttcagccatt actcacttgg
    27361 ctgaaaaact cggattgacc aaagatgaaa ccatggcaat cggtgatgaa gaaaatgacc
    27421 gtcccatgct ggaagtcgtt ggaaaccccg ttgtcatgga aaatggaaat ccagaaatca
    27481 aaaaaatcgc caaatacatc accaaaacaa atgacgaatc cggcgttgcc catgccatcc
    27541 gaacatgggt actgtaaaag tatcattttt caataagaat tgattagcaa taaaatccaa
    27601 tgaatttttt tagcaaacta tttaatttaa aacaaaataa tcataataga gacacaaatt
    27661 ctgattgtaa caatttttac ctaaacgaat tagaatgtgg ccttactcct gggcaactca
    27721 tactcataga ttggactcaa aaaacaggga gaaattataa tttcccaaga tattttaaat
    27781 actctcttca aattgaccct gaatctacac acaatcaatt atacaaatta ggatacttca
    27841 ctaaaaataa gactttatca tatcttacag tagtagaatt aaaaactata ttatctaaac
    27901 ataatttagc tacttctgga aaaaaagcag aattaattac aagaataatt aataatgtta
    27961 acattgacaa tttagatatt ccgttcgaat ttaaactaac aaaagaagca caaaatctta
    28021 ttatcgaaca tagtgactat atcaaagcat actatgataa agacataagt atggaagatt
    28081 attgtaaaga aaaaaacaat atctctttta aagcaacttt tggtgatata aaatggagtc
    28141 tcttaaataa acaagctcat aggaatactg tatcaggaga ttttggatgc ttatctaaca
    28201 cacgaaaggc tcagggaaga catttggaac aagaaggtaa tattaaacat gctttaatat
    28261 attacataga atctttgata attactattt caggattaga aaacaatttt tcagccactg
    28321 attatccagt atattatccc gattcgatac ctgactactc actaaaacat attcaaacat
    28381 taatggaatc attatctgat gacgattatg attttgcttt tgatgaagca ttatttcgct
    28441 tctcaatttt gaatgcaaat cattttttat ctaaggaaga tattgactat ttaagagtta
    28501 atttacctcg ttccactgct gaagaaataa acaattactt aaagaaatat gaatgttata
    28561 gtcctttaaa taatttagaa cttgacgatt ttgaataaat tgactataca aacatttata
    28621 tactcgatat agtctcaatt ttatctgatg attgcccaaa tttttcaata ataaaacgca
    28681 taatattatg gagacaatcc cctatattat gcgttctttt aatatcaaag actctttgac
    28741 aaacttcttt gatatctaat tacatgcccc ctgcaggaat cgaacctgca actactcctt
    28801 aggagggagt tgttatatcc attgaactaa gggagctaga taaaaactct gctgaaaaag
    28861 cagagttttt tagtcgaatt aacgacggat ttctttgata cgagctgcct taccttgaag
    28921 agcacgcaag tagtacaatt tcgcacgacg tactttaccg taacgtacaa cttcgatttt
    28981 ttcaacacgt ggagtgtgga ttgggaagat acgctcaaca cctacaccgt tagagatttt
    29041 acgaactgtg tagttttcag agatgccagc acctttacgt gcaataacaa caccttcaaa
    29101 aatctggata cgttcacggt taccttcgac aactttcgca tgtacacgaa cagtgtcacc
    29161 aggacggaat gatgggatat ctgtacgaag ttgaccttca gtcaagcttt ggattaatgg
    29221 attcatttta ttctcctatc tttgtcaatc ttgaggaatc ttcctcagcg gataaactgt
    29281 atttttgtgc gtccattaca cacaagatat agtttaccaa atttctaaaa aaaagtaaag
    29341 aaatttatag caaaattcct aaaaaaatcg caaccaaacc acctatgtaa gtcaaagtca
    29401 ggtaagaata aaagaccttc ttatcactta gcagtctttg aagttcgtca ttcaaggtcg
    29461 aaaaagttgt taaaccgcca caaaatcctg ttgctagaat agcatatact tccttagact
    29521 ctacatgatt gtagaatact ccaattaaaa aacaacctaa aagattagct atgagcgttc
    29581 cgagtggcaa tttagaagct tgattatagc gagaaaagaa ataccgtacg agtgctccca
    29641 agccacaggc gattgcaaga tagacgatta ccatttcttc ctccccaaat agtaagctaa
    29701 aagcaggcct ccaccgatac tcaaaagcaa atacaggact aaactaaaat aacgccctgt
    29761 atcaagcagc ttcacagtat caagcattag gctggaaaaa gttgttaaac ctccgcaaaa
    29821 acccgtcccc agtgctaaaa tcaagccctt actagtcccc ttatagacca gatagccctt
    29881 gacaagaaaa atcaagcaga aaattcccag atagttgaca aagagagtgc cccaaggaaa
    29941 gtctggactg gctggtaacc aggtggaaac tagatatcgg acaagtccgc ccaacatagc
    30001 agctagaaaa attcctagcg gataaaattg ttcttttttc atttgatatt ttgatcctga
    30061 taatcacgcg aacgtttgag tatgtacgaa aaagtcgcga caatagtctc ctgatagcgc
    30121 ttgtcctcta cacgactcct aaccttttca aaaataactt cttctctctt agtatctaaa
    30181 atcggtttac ctgaagcttt cttataagca accaccccct caactaaatg cattcgttct
    30241 tctaacagtt tgacaatttg gtcgtcgatt tgatcgattt cttgacgaat aatatctaaa
    30301 tccatacagt ctcctccttt atttgaatta ttgtatcaaa aagccatcaa ttaggctagt
    30361 aaaatcccaa ctctcgataa gaaaaatgca ctgattgatt acatcagcgc acttttatgc
    30421 ttatcctact ttagcagcca attcttctgc gaattgttcc aagcgttcaa tatcttcttc
    30481 ctcggcagaa agatcaactt taacacactc tgaacctttt tctgctcctg ttgacacaaa
    30541 gacacggtca aagtcatcaa cagccttaca gaattcatcg tagaaggtat ctcctgagcc
    30601 gaccactccg tagattttgc cattcaagtt gagatctgct aggtcttcgt agaagtccat
    30661 catctcatct ggcaattcac catcaccata agtataggtc gcaacgatag cgatgtctgc
    30721 ttccaagaag tctgaagcgt caacagttgt acattcatca acatcaacat ccaagcccaa
    30781 gtcacgtaat ttgtctgcta caatatctgc aatttcttcg gtattaccgg tcatactggc
    30841 aaatacaatt tttgctaatg ccatatcgtc ctcctcaatt tatctttctc cattatatca
    30901 catctttttc attttaaaaa taaaaattct tgtgctacaa tgaaatgaga aagaattgag
    30961 gttatttatg gagatttgcc aacaaatttt agaaaaaatt aaagaataca acaccattat
    31021 cattcatcgt catatgaaac cagaccctga tgccttggga agtcaggtgg gattgaaagc
    31081 cttgctggaa catcatttcc cagaaaaaac catcaaagcc gtcggttttg atgaaccaac
    31141 tcttacttgg atggctgaga tggatcttgt tgaaga
//
LOCUS       DAVBCW010000012        31040 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVBCW010000012 DAVBCW010000000
VERSION     DAVBCW010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31040
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.012"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            1..235
                     /locus_tag="VQ514_000479"
                     /pseudo
     CDS             1..235
                     /locus_tag="VQ514_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(363..1385)
                     /gene="cbpF"
                     /locus_tag="VQ514_000480"
                     /pseudo
     CDS             complement(363..1385)
                     /gene="cbpF"
                     /locus_tag="VQ514_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpF"
     gene            complement(1404..2225)
                     /gene="cbpG"
                     /locus_tag="VQ514_000481"
     CDS             complement(1404..2225)
                     /gene="cbpG"
                     /locus_tag="VQ514_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpG"
                     /protein_id="HET2499292.1"
                     /translation="MNVEGRGSANFIKDNVLITAAHNYYRHDYGKEADDIYVLPAVSP
                     SQELFGKIKVKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLGTLGLPTSQKNLT
                     GITVTITGYPSYNFKIHQMYTDKKQVLSDDGMFLDYQVDTLEGSSGSTVYDASHRVVG
                     VHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGWKKINGSWYYYRQHDKQTGWQE
                     INDTWYYLDSSGKMLTDWQKVNRKWYYLNSNGAMVTGSQTIDGKVYNFASSGEWI"
     gene            complement(2306..2545)
                     /locus_tag="VQ514_000482"
     CDS             complement(2306..2545)
                     /locus_tag="VQ514_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499293.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPESNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(2695..2961)
                     /locus_tag="VQ514_000483"
                     /pseudo
     CDS             complement(2695..2961)
                     /locus_tag="VQ514_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(2986..3297)
                     /locus_tag="VQ514_000484"
     CDS             complement(2986..3297)
                     /locus_tag="VQ514_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HET2499294.1"
                     /translation="MDWDFADTCWEKEPRKYQYVAANYLKAMQSYLKETDLPKLEQLV
                     VTKSWWDTVDILDRVVGSLVYEHPELEEIILILFENLFKPRQLYLQPQNSVLSNLRLA
                     S"
     gene            complement(3222..3464)
                     /locus_tag="VQ514_000485"
                     /pseudo
     CDS             complement(3222..3464)
                     /locus_tag="VQ514_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(3465..4097)
                     /locus_tag="VQ514_000486"
     CDS             complement(3465..4097)
                     /locus_tag="VQ514_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2499295.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGQEEF"
     gene            complement(4111..5106)
                     /locus_tag="VQ514_000487"
     CDS             complement(4111..5106)
                     /locus_tag="VQ514_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2499296.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FFFLLLVFSMSMTCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVVDPDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQTQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(5103..5801)
                     /locus_tag="VQ514_000488"
     CDS             complement(5103..5801)
                     /locus_tag="VQ514_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HET2499297.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(5879..6889)
                     /locus_tag="VQ514_000489"
     CDS             complement(5879..6889)
                     /locus_tag="VQ514_000489"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HET2499298.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKAVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEAKDQMK
                     KA"
     gene            complement(6873..7880)
                     /locus_tag="VQ514_000490"
     CDS             complement(6873..7880)
                     /locus_tag="VQ514_000490"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HET2499299.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLTPNPDYSLIQETIALMGDFLAVRGQNLRSFSLEICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYDVSVDQELLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKVAAWLEEENLATVLERDWGFSISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLSSSKETVVSLVEALEQGKSEKIIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(7867..8820)
                     /gene="mvaD"
                     /locus_tag="VQ514_000491"
     CDS             complement(7867..8820)
                     /gene="mvaD"
                     /locus_tag="VQ514_000491"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HET2499300.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVIADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YSVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGQRYRLIVSKTKDLSQDDCC"
     gene            complement(8802..9680)
                     /gene="mvk"
                     /locus_tag="VQ514_000492"
     CDS             complement(8802..9680)
                     /gene="mvk"
                     /locus_tag="VQ514_000492"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HET2499301.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLDITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(9802..10131)
                     /locus_tag="VQ514_000493"
     CDS             complement(9802..10131)
                     /locus_tag="VQ514_000493"
                     /inference="COORDINATES: protein motif:HMM:NF024736.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2499302.1"
                     /translation="MTRKAYDTDLNDQEWAKIEPYFSKHRTYKWPKRVLVNETLYVTK
                     TGCQWRMIPHDFPLYLTVWSFFRRSMTTGWFQVNGRWYYAYSSGALAVNTTVDGYSVN
                     YNGEWVQ"
     gene            10793..11129
                     /locus_tag="VQ514_000494"
                     /pseudo
     CDS             10793..11129
                     /locus_tag="VQ514_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811282.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic-modifying protein"
     gene            11029..11616
                     /locus_tag="VQ514_000495"
     CDS             11029..11616
                     /locus_tag="VQ514_000495"
                     /inference="COORDINATES: protein motif:HMM:NF017000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499303.1"
                     /translation="MTILLKLSSTIVYGEIYHYFLQRDTAKESILDYSFAHGYCEIAY
                     ALFAYSKVLEPSMFYNDLHTFHAELKKLLEKVTSNTENLGNLQLSWCEGISGIILYLC
                     MYDCDGNKDIISKYQEFVFNHHLKMMTGYCHGITSLLQTTVYNQNKLLMKKIQQVILA
                     CSERDDHGLLMFQGDSGKADLFDFGIGSRGYIGVY"
     gene            11659..12891
                     /locus_tag="VQ514_000496"
     CDS             11659..12891
                     /locus_tag="VQ514_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2499304.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALCIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVHIVANLAGA
                     FLINVISIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLFALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLALITSILCQAYIVIFVTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(13009..13965)
                     /gene="cbpC"
                     /locus_tag="VQ514_000497"
     CDS             complement(13009..13965)
                     /gene="cbpC"
                     /locus_tag="VQ514_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HET2499305.1"
                     /translation="MKLLKKMMQVLLAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIPGTGYRDDLFDNRPVFEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     DGNWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLRSSGAMA
                     TGWYQEGTTWYYLDQPNGDMKTGWQNLGNKWYYLRSSGAMATGWYQDGSTWYYLNAGN
                     GDMKTGWFQVNGNWYYAYSSGALAVNTTVDGYSVNYNGEWVR"
     gene            complement(14063..14752)
                     /locus_tag="VQ514_000498"
     CDS             complement(14063..14752)
                     /locus_tag="VQ514_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2499306.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLILLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVFIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            complement(14764..16188)
                     /gene="gndA"
                     /locus_tag="VQ514_000499"
     CDS             complement(14764..16188)
                     /gene="gndA"
                     /locus_tag="VQ514_000499"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HET2499307.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVSDVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEDQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            complement(16264..17658)
                     /gene="mapZ"
                     /locus_tag="VQ514_000500"
     CDS             complement(16264..17658)
                     /gene="mapZ"
                     /locus_tag="VQ514_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HET2499308.1"
                     /translation="MSKKRRNRHKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTNSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLVTQIKAIQDVNAQFEKPAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            complement(17671..18828)
                     /locus_tag="VQ514_000501"
     CDS             complement(17671..18828)
                     /locus_tag="VQ514_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HET2499309.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARLPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVERKLELDIMGCDIDARMVEIAKANA
                     QAAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            complement(18913..19293)
                     /gene="rnpB"
                     /locus_tag="VQ514_000502"
     ncRNA           complement(18913..19293)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VQ514_000502"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            complement(19314..19643)
                     /gene="gpsB"
                     /locus_tag="VQ514_000503"
     CDS             complement(19314..19643)
                     /gene="gpsB"
                     /locus_tag="VQ514_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HET2499310.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            complement(19713..20240)
                     /locus_tag="VQ514_000504"
     CDS             complement(19713..20240)
                     /locus_tag="VQ514_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HET2499311.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDQLNELAENFSEN"
     gene            20306..20902
                     /gene="recU"
                     /locus_tag="VQ514_000505"
     CDS             20306..20902
                     /gene="recU"
                     /locus_tag="VQ514_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HET2499312.1"
                     /translation="MINYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLTVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            20899..23058
                     /locus_tag="VQ514_000506"
     CDS             20899..23058
                     /locus_tag="VQ514_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HET2499313.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGIYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLTYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWSSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(23225..28528)
                     /locus_tag="VQ514_000507"
     CDS             complement(23225..28528)
                     /locus_tag="VQ514_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HET2499314.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATANENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQQGTVTIREEKGVRYNQLFSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKEDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKTPGNSTWYKGNRVVAPEPGSVNRLSITLKSDGQLNASNNDVNLFDTVTLPGAVN
                     DNLKNEKKILLKAGTYGNDRTVVSVKTDNQEGVKADDTPAQKETGPVVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYSLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKLRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTDKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRNNATVNDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDQAT
                     YFDEIRTFENNSSMYGDKHDTGQGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVADD
                     FASLTAPAQAQEGLANAFDGNLSSLWHTSWNGGDVGKPATMVLKEPTEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLSSLVSDQGKTPDYKQEIA
                     RPETPEQILPATGESQSDTALFLASVSLALSALFVVKTKKD"
     gene            complement(28829..30811)
                     /locus_tag="VQ514_000508"
     CDS             complement(28829..30811)
                     /locus_tag="VQ514_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2499315.1"
                     /translation="MKSSKLLALAGVTLLAATTLAACSGSGSSTKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDDQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHVDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQGSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNIFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
BASE COUNT         8910 a         7038 c         5555 g         9537 t
ORIGIN      
        1 ttagaacaag tacaaacaat gttggaacag gcattcacag agaagcacta tggtggagtc
       61 cttctttggg attctgaaat cggaaatgtt ttatggctat gagaaaacat ttaaatcact
      121 taatcaattg gaacaagcta ttatagacta tattgattat tacaacaaca agagaattaa
      181 ggtaaaacta aaaggactta gtcctgtgca gtacagaact aaatcctttg gataaattat
      241 ttgtctaact tttggggggc agtacagaaa aaccttgata ctatgcgttt tttgattgtg
      301 aagactttta tccacctatt atacaaagtt aagtatccat cacagtttac aattagcctt
      361 cattacttaa cccattcacc attatagttt aagtagtaac cacctactgt ggtattaaca
      421 gctaaagcac ctgaatcata ggcatagtac cagttaccat tgacttggaa ccagcctgtt
      481 ttcatatctc cattacttgc atttagatag taccaagtcg aaccttcctg ataccaacca
      541 gttgccatag ctcctgatga acggagatag taccacttgt taccaaggta ttgccagcca
      601 gttctcatat caccattttc agcatctaga tagtaccaag ttgagccttc cttataccag
      661 ccagttgcca tagctcctga cgaatggagg tagtaccatc tattacctag aaattgccaa
      721 ccagtttgca taatgccagt tgttggatct agatagtacc atggagctgc ttttagcttc
      781 tcttcatcat acgtaagagg ggaatcctta acccaaccac atgctagctc tccaaccgcc
      841 aatctgttga tgcgcgaatc aaagccacca tccttctgta aataatacca atgaccctct
      901 tcgtaaatcc aaccagtttt taaagtatga taactacgct gatcttcaaa ataatagact
      961 catttctctg ctgggctatc atattcttcc ccatgatgtt tgttggtatt cgtagcagtt
     1021 tttgcttcta aaacttgctt gccaacaaat tcttgtaata caccatcttg accaaaataa
     1081 aaccaatctg gtctaagaga aatctctatt cttggagaag gaccaatcgt aacccccttg
     1141 tgtggagcag gtatatattg ccagccgaca accatctctc cggataaagg atcaaaataa
     1201 tagtacttcc catctatcac tctccagtag gtttctttta gatccccctt tttgtagtag
     1261 gttctaccat tttcttggac aaactgccat ccttcagaat catctgcaaa tactgtactg
     1321 gtccctagca aaccgaagaa aaatgtggct agtgcgactt gcatcatttt tttcaaaagc
     1381 ttcattttat atatcctcca acattaaatc cactcaccag atgaagcgaa gttataaact
     1441 ttaccatcga tagtttggct acctgtaacc attgctccat ttgaattgag ataataccat
     1501 tttctattta ctttttgcca atctgtaagc atcttaccgg aactgtctaa ataataccaa
     1561 gtatcattta tctcctgcca acccgtttgt ttatcatgtt gtctataata gtaccaacta
     1621 ccatttattt tcttccatcc ttcaagagag taacctttaa gaaccgaata aataaatggc
     1681 aaatttcgtt catttaattt aactgcactg ttaatttgat tagctccatc tcctaaagta
     1741 tgcactccta ctacacggtg actagcatca taaactgtag atccactaga cccctctaaa
     1801 gtatcaactt ggtaatccaa gaacatgcca tcatcactta aaacttgttt tttatctgta
     1861 tacatttgat gaattttaaa attatatgat ggatagcctg tgatagtcac agttattcct
     1921 gtcaaatttt tttgactagt aggaagaccc aaagtcccta attttgcacc aatgggctct
     1981 tctagaatta ataaagccaa gtcatattcc cttgcatcct tagaatttaa atttctaaat
     2041 tccttcaaat aacgaacttc ctttactttg atctttccaa atagttcttg acttggacta
     2101 acagccggaa gaacataaat atcatccgct tctttcccat agtcatgtct gtagtagttg
     2161 tgagccgctg taattaacac attatcttta ataaaattcg cacttcccct cccttctaca
     2221 ttcattccat cggctactcc ataatactta gataaaacca tagctgaagt cgaatacgga
     2281 tactgtaaag tattatcaat tttaatcaaa tcatcattac cgataatact tctgattgct
     2341 tttggtagta tgaaccatac gttggtgaaa tctcagataa tgaagaatca ttagactctg
     2401 gacctttttc tagtgtctca cttacctcat attcttcacc tgtactagaa ataacactca
     2461 aagcagatac tgtcgataac tggctagcca ataaagtact cgcaataatt gaaataccca
     2521 attttttata aacagttttc ttcattattg tatcctccta atgtaattat agcgtactat
     2581 tctaaatttc ttaatctact atagaatcaa gaaatctacc accttcttta aataccctcc
     2641 attatcacat aaacaggtaa acttttcaat taatgactgc gcttttcaat cacgctagag
     2701 gtacttactt gcttctttga tactaagttc agccattctt tccttgtttt catcaataaa
     2761 gcatgttacc caagttggat tcgttttgga gtagtctcgc agaatccagc caatggcttt
     2821 attgataaaa aattctgttt ggttcaagtt atgaagtaga atcttttcca ttaattgagt
     2881 attggtcttc tcttttctta acaactggtg gtcaatagcg acacgtctca gccagatatt
     2941 atctgatagg ctccatttta tactcaatga aaatcaaaga gcaaactagg aagctagccg
     3001 cagattgctc aaaacactgt tttgaggttg cagatagagc tgacgtggtt tgaagagatt
     3061 ttcgaagagt attaagatta tttcttctag ttcagggtgt tcatacacca aactccctac
     3121 tactcgatct aggatatcta ccgtgtccca ccaggatttt gtcacgacta actgctctag
     3181 cttaggcaaa tcggtttcct ttagataaga ctgcattgct ttcaaatagt tagcagccac
     3241 atattggtat tttctaggct ccttttccca gcaagtgtct gcaaaatccc aatcgataat
     3301 ctttgttttt ttcgcttcta gtaaatattt ttatagagtt tatttctttc aggcaccgca
     3361 atacctagaa aagaaaattg atggcgcata taggcttcca tgggccttgc ttttttagag
     3421 tcttttgctg cttctagctc ctcaagtaaa tctgctaaac tcatctaaaa ctcctcttgc
     3481 cccaccaaat ggtgctgaaa ggcatagaca gccgcctggg tacgatcgct gacttcaagt
     3541 ttggcaagaa tattggacac gtgggtcttg accgtcttga gagagataaa gaggtcatct
     3601 gcgatgcgct gattttcgta gcccttggcg atgagttgga gaacatctcg ctcacgcgca
     3661 gtcaattctt catgaagttc catatgattg cggtggtatt caaccttctt gctaacctct
     3721 tgctcaatgg ccagctcgcc agcagctacc ttactgacgg catgaagcaa ttcatctgca
     3781 ctagaagtct tgagcatata gcctttggca ccagcatcta agactggcat gattttttca
     3841 ttgtccaaat aagaggtcac aatcaaaatc ttggcttcag gccattcttt aaggattgct
     3901 aaggtcgcgt caattccatt catctcaggc atgacaatat ccatgacaat gacatctgga
     3961 cgcagttcca aggccaagtc aatcccttga gacccgttgg acgcctcacc cacaacttct
     4021 acatcgtctt ggaggtcaaa gtagcttttc aagcccaatc ggaccatttc atggtcatct
     4081 actagtaaaa ttttcatctt tactccttta tcattcctta tctaacaggg gaatacggat
     4141 atcaaccgcc agcccttgct tgggagctgt caagagttga actgttccag ccatatcttc
     4201 aacccgctcc ttgatatttc gcagtccata actcaagtcg tctaagctcc ctaactggaa
     4261 accaatccca ttgtccacca ccttcagttg caattcaaca tctgtctgat agaggtagac
     4321 atctaggcaa gatgcctggg catggcggag ggtattgcta atcaactctt gcaggatacg
     4381 gaagatatgc tcctcgattt tcttaggcaa tttcgtcata ttctgcttga gactaaccct
     4441 aagatcactc ttgtcctcaa gctcttttaa aagaatttga atcccttcta tcaagctctt
     4501 ctgctccagt tcaactggtc gcaaatgcaa gagcaaaacc cgcaaatcct tctgggctgt
     4561 ttctaaaata gctgtgacac tctgcaactg ggtctgcatc ttttctctat ccaatttcaa
     4621 agcctgctga ctgatacccg ataaaatcat gtgggccgca aacaactcct gactgactgt
     4681 atcgtgcaaa tcccgagcaa ttcgcttccg ttccttctcg atgatttcct cttcctgagc
     4741 aaggctatga ttttcagctt tttgaagagc ctctgtcaaa aggttaagtt tacctgataa
     4801 ggacttgaaa ctggcatcca aatctggatc tacaacctga accacttctt gccctgccaa
     4861 taaacgcttg agattagcct gcatttttct tagagaaagc tcttcgatcc ctcgccaaaa
     4921 cagggctaag agacaggtca tggacatgct gaaaaccaac aataaaaaga aaaatttttc
     4981 tgttttttcg acatcgtgca aaaagataga ccagtcaaaa tcaagtattt ccagcaagct
     5041 atgggagaaa aaaaagacaa acaggaagga ggtgagagcg ataatgacat aggcttgttt
     5101 tttcatcctc taaccacctc aacatcacca atcatagtgg tcaagaaaat cttgacactc
     5161 ttgttactct tgagatagtc ttttgtttct tgatgatagt gttcattgcg gagggctcgc
     5221 ttgggctggt tgaaaaaagt caaatcccca tagagacagt taacgctgag actgacttcc
     5281 acatctacag gtacgatgat tttggtcgtt cctaccatct ttctgaggat aatgacattg
     5341 tcatgattgg ttaagatgac tctctccaga tgaatagtgt ccttgcccat gaagcgaaag
     5401 agattgatat catcaaattg gcaagtctgg tagcttgaaa aatgatgaag atttccaaac
     5461 caacgatttt tctccttctt aaccgtcaca acctcttcaa aaaccaaatt ggtctgctct
     5521 ttttcctggt tcatcatcgg gtaaagaaga aagagactat agataaccgc aacaaaaata
     5581 gctagaatca caaaaggatt gagcataacg atgaaaaaga agagaatggt tgccgctact
     5641 aaaagaagat tattcccctc tttaccagta tagtagcgaa tcaaaagcaa aaagaggaat
     5701 agtatcagca gaaaacgcga aaaatgctct gataccatca aaatcagagc tcctgtcaga
     5761 agacaggctt cgataaataa aaagatttta aattttctca taggttcatc ctctcccttc
     5821 tattttatca caattcaaaa aagtcacctc agtctgagga tggaaaaaaa ggcgctggtt
     5881 acgccttttt catctgatcc tttgcttctt ttaattttcc ataaagaaga tagtctactt
     5941 tttgtagatc tgctatggtg gcacagttaa gggaacacat aatcaagcgt agatctgctt
     6001 tccagccttg gacaatgcca atcacttctt caactgtgta ggtttcaacc aattccagaa
     6061 cggttcgtga caatcccaca gccttagcac caaaaaccaa gcacttaatc atatccagcg
     6121 gattccgaac ccctccacta accaagagtt cgaccttatc tttccattct tgggcattga
     6181 gaagggcctg catggtagac tgaccccatt gattgaggta atcacgctgg ccactacgac
     6241 ggttttcgat ataggcaaag ctggtgccac cacgacccga taggtccact gtacgaacac
     6301 cgaattcata ggctctttcg attgtcttgg catccattcc aaagcccact tccttgagga
     6361 caataggaac gggaatttgc ttgctataat ctgctagatg cgattgccag cttctaaact
     6421 tcctttctcc ctcgggcatg agtaattcct gcatgacatt gacatgcact tgcaatagaa
     6481 caggattcat ctcttctaca gtctgaagtc ctaactcgac aggtttgtcc aatccaatat
     6541 tggttccaag gaggagattg ggatgactag acttgacaga aaaagaatca tccgttggat
     6601 ttttgagggc tgcgctataa gaacccgtta caaataaaat accacaggat tccgccacct
     6661 gagccagctt ttgattgatt tctcttccct tattacttcc accagtcatg gcattgatat
     6721 aaaaaggaaa gtcccacttt cgaccagcaa actctgtcga aagatcgatt tcatccagat
     6781 tgtaaagagg caaggaagaa tgaatcagct ccacctcatc aaagctatta taggaacttt
     6841 tctgctcaag ggcatagagg atatgctcgt ccttacgatt tgtcgtcatg tcctatcctt
     6901 tcttgatata agagctcaat ccccagatcg gcccaacgat tttttaaggt tttggttgat
     6961 tgcgcatcaa aactcagggc gatgccacag tcaccaccac cagcaccact actcttggca
     7021 acggcctgca aatcttgact ggcttctttc aactgtctaa gcaaaggcgt gtaaatatct
     7081 gtactcaagc cttctaaaag cttgctggct acttctactt gctcgataat cttttctgat
     7141 ttcccctgtt ccaaggcttc gaccaaagaa accaccgttt cttttgagga acttaaaaaa
     7201 ttttgattga tattttgctt gatttgctgg accatgtgac tcgatacagc cacttccttg
     7261 gtccatccca ctaagaaatc acattctaaa gttggtttca cttgtgaaat tgaaaagccc
     7321 caatcacgct ccagaactgt cgccaagttt tcttcttcta accaagcagc caccttctgg
     7381 cgatcaaatg actggtagag aaccaaatcc tctgccacaa tacaggcaag gtcgcccatg
     7441 gaaccattgt ctcctcgctt gagcaagaca gcgctagtca gcttgaacaa gagctcctga
     7501 tcaacagaaa catcatacag agccagtaaa gccttgacaa ccaagacaac gacgctgcca
     7561 ctagaaccta gaccaaactt tttcccttct cgttccattt tgccacagat ttctagggaa
     7621 aaagatctta aattctgacc gcgaacagcg aggaagtctc ccatcaaagc aatcgtttct
     7681 tgaatcaagc tgtagtcagg attgggcgtt aagtccactg cgaaatcaaa catatctgaa
     7741 tagatacggt agctgtcaga aaaagcaatc tcagccctca tatagatggg aatatccttt
     7801 atcaaagcta actgccctgg ctctaaaata gcatattcac ctgcccaata gagttttccg
     7861 caagttttaa cagcaatcat cttgactcaa atcctttgtt tttgacacaa tcaagcgata
     7921 acgctgaccg aaaatttctg acaaatgctc caagtctttc tcctgacaaa agaccttaac
     7981 attgggacca gcatccatgg taaagtagca ggcctctcct ttctcacgaa gctggcgaac
     8041 aaagtccata gcctcataag aggcatccgt cagataagaa aaggctggac tagcagtctt
     8101 tgtcgtagca tgcatagcca gggcattttt ctccgttaat tctccaatct tggcaaaatc
     8161 attttccttg agataaatca gcatatcctg atagtccttc tcagactgac gaacccagtc
     8221 gtcaaaagtc gtcgaggttt ccacacaaag tttcatcccg tcacggctag agattggttt
     8281 tttcttgtcc tctagcacca acataatcat agctagtttc aagtctgtct ctacagagta
     8341 aatttctcca ctatccttat cccaggctcc tagcggtcca taaaaactcc gagaagaaga
     8401 gcctgaggca aacttggctt cctgcgctaa ctgacttcta tccaatccaa gcttgaaata
     8461 agcattacaa gccttgacca gggcggacaa accactagaa cttgaggaca ggcccgctgc
     8521 agtaggcata ttgttttgag tatcgatacg gacaaagccc tcaccagctg gacgataacg
     8581 gtcaataatc ttactcatct tggcatgctc gacctcattt tgtagctgac cattgatgta
     8641 aaattcgtca gctattacat tggctggtaa aggcgacaag gtcgtctctg tatacatatt
     8701 ttccaaagtt agagaaatac tgctagtagc aggcaccatc tctttttctt ttttctttcc
     8761 ccaatatttg ataatagcaa tatttgcgta ggaacgtact gtcacaggct ctctatccat
     8821 gtctgaacag ctcctttctc ttctaatctt tctgctagtt cttgtgcgtg tgtcaaattg
     8881 gttaccaagg ctatgataca acctcctagc ccaccaccgc tcatcttggc acccagagca
     8941 ccatggctaa gagtcgtttc aaccaaaaag tctgcctcag ggctactgac tccaatttct
     9001 tttaaatgta aatgcgcttg actgaggatt tgtcccagtc cttcagcatc tttttgtgaa
     9061 atcgcaactt ctgcttgctg ggttaattct cccaaggcat gcaaaaacgg tagggcatcc
     9121 ttgcccttat tttgaaccac ttggatggct tcacgagtat gaccataaac acccgtatcg
     9181 gcaatcacca aataggcgga taaatccatc tcaagttctg taaatcctac gttcttgata
     9241 aagcgaatag gttggtcact gagacaggtc ttagcatcca aaccactagg attcatatgg
     9301 gcaatcatct cagctcgatt gaccaagatt tctagtacat catgaggcag atcagcctga
     9361 tagtagtcaa ataccgcacg aatggccgct atgctgatag ccgctgacga acccatcccc
     9421 cgtttctcag ggatagccga gtcaatctca caacgaatgc aggcttctgt gatatccaaa
     9481 tactccagtg aggcataaac cgccatggac aaggtatcct cctcataaag gcgccaagga
     9541 ctctctgcag gaactacctt acaggtcacc tccacctcca aaagaggcag ggaaatggca
     9601 ggataaccgt aaacgaccgc atgttcccct attaaaatta tcttactatg tgcctgaccg
     9661 acaccaactt tttttgtcat tttttccttt tactagacga aaaaacgtct tatttttcat
     9721 acaagtatta attctttcct atctatttta ttatattttc acaaaaaaag cgattgtttc
     9781 cattcacaat cgcttctttc attattgaac ccattcgcca ttatagttga cagaatagcc
     9841 atctacggtc gtattcactg ccaaggcacc tgagctataa gcgtagtacc atctgccatt
     9901 gacctggaac caacctgtcg tcatagaacg acgaaagaaa ctccataccg ttaagtaaag
     9961 aggaaagtcg tgagggatca tgcgccattg acaacctgtt ttagtgacgt acaaagtctc
    10021 attaacaagt actcgtttcg gccatttata ggtgcggtgt ttggagaaat agggttcaat
    10081 cttagcccat tcttgatcgt ttaaatcagt atcatatgct ttgcgtgtca taactctagt
    10141 ttaacatttt ttgtgaatac aagttctaaa taatcgacca cgaaaatttc ttaagtggaa
    10201 aacgccttat gaagtatgct acgggaaact tatgcactta atttgacaat tcaagatgta
    10261 aaaatatata ctatagtaga ttgaaactag aatagtacac ctctacttct aaaacatttt
    10321 tagaaatcga tttgactgtc ctgatcgatt tgtcctgtta ttattttatt ttactataat
    10381 atttgacaag ttatcctaaa agtattatta tgttgttgtg ttatagattg attgaatcta
    10441 actaaaggat cctattcaat tactagaact atcacatact caaggtcagc tcacagatga
    10501 gcaactattt tggttacaat gtctactaaa tttaagtcaa acaaataatt tagtcaaaat
    10561 taaaaaaata gaggaacata aatatgatta caaaacagaa tgtaatagtg ttctacgatt
    10621 tttactagat aaaactgtaa attctgaagg aaggatcact tcttcaacag aatttggaaa
    10681 tttcgtaagt aatttatcat tccaacacgg aatagctgga ctactgtttc ctctaaataa
    10741 attgtacccc ccagaactgg attctaaaat actctctatc atcaagaagg cagtgacaat
    10801 tagaacgaca cacatatgaa tatcaatact cactgctatt tggtgatgca ggctatctat
    10861 ggttactcct acatttattt tctatcagta aaaatcaata ctatctacaa ttagcaaacg
    10921 tcaccgctaa gaaattaata gagaattatg atactctaga agaaatagac tttgcattgg
    10981 gaaaatctgg tgtcctatta tcattaataa aatactatca atttaccaat gacaatactc
    11041 ttaaaacttt catccacaat agtatatggg gaaatttatc attatttcct acaaagagat
    11101 acagccaaag aaagcatttt agactatagc tttgctcatg gatattgtga aattgcatat
    11161 gctttatttg cctattctaa agtcttagaa ccttctatgt tctataatga tctccataca
    11221 ttccatgctg aattaaaaaa attattagaa aaagttactt ctaatactga aaatttagga
    11281 aatttacaac tttcttggtg cgaaggaatt tccggaataa tcttatatct ttgtatgtac
    11341 gattgtgacg gaaacaaaga tattattagt aaatatcaag aatttgtttt taaccatcat
    11401 ctaaaaatga tgacaggata ttgccacgga ataactagct tactacaaac cactgtctac
    11461 aatcaaaaca aattactgat gaaaaaaatc caacaggtaa ttttagcatg ttctgaacga
    11521 gatgatcacg gtttactgat gtttcaagga gatagtggta aagcagattt gtttgacttc
    11581 ggaataggaa gcagggggta tattggtgtc tattaaataa taaattccca ttcgatgtgc
    11641 aaacataagg agaaatgtat gaaattattt tggacaaaca acatatatag acagttgctg
    11701 ctaaacagct gtttttcatc attcggcgac agtattttct acctcgccat tatcaattat
    11761 gtggctcagt acaatttcgc tccgctagcg attttactga tttccatttc agagatggtt
    11821 cccctactat cgcaactctt tctcgggatt ctaggagatt ttcaagaaaa tagagtcaaa
    11881 cacgcactct gtattgccaa aatcaaaatc ctgctctacg ctattttgac agtatttctc
    11941 gtcttgtcgc ccttttcatt agtttcagtc attatgattg tcatcatcaa cctcatctct
    12001 gacaccttga gctacctgtc tgcctacatg atgaacgccc tctacatcag tgtaattaag
    12061 gatgacctgc atgatgccat ggggttcagg cagtctctga tgagggttgt ccacattgtc
    12121 gccaatctgg ctggcgcatt ccttatcaat gttataagta ttcaaactat ttcccttatc
    12181 aacactctga cttttgtcat tgcctttttg ggcctgtatg ttattcgaca taccttgtat
    12241 gaggttgaaa aaaggattga aatgtcacat acagcactga gttttaagaa atattttcaa
    12301 catcttaaac agtcgctggc tgtgctcctg aggttaaaag ataccgtcat actactgttt
    12361 ctgacgacca gtatgattgc catcttggat gtgtcccctc ggctgattgc cctccgcttc
    12421 atccaacaga cactagcaca actgagcatt gggcaactct tcgccctgct ctccatcatc
    12481 atgtcttgtg gagctatcct tggcaatatg accagcagta atctatttaa aaatatccgt
    12541 ttcacgcacc tcttggtttt ctgtgagatt tccctattgg ctctaataac tagtatcctt
    12601 tgtcaagcct atatcgtaat tttcgtgacc agtttcatca gttctacgat tatcggcatt
    12661 ctcagccctc gcctacaagc agctgtcttt gcccatatcc ccagtgacaa gatggggacg
    12721 gtgggctctg ctctgagcac agtagacatt ctcgccccgt ccctgctctc cctattagcc
    12781 ctatccatag catcgggcgt ttcggtgcag ttagcattga tatttttgta tcttattttg
    12841 attgctctta tcttttgtca atggttagtc aagttcaaca ctcataacta acgaaaaagc
    12901 atgtgtagat ttcacatgct tttaatctcc ccaatcgtca ggtcaagtac aacaaagtca
    12961 cttctttgat taagcgagtg ttctaatata attataagcg ccctgtcatt accgaaccca
    13021 ttcgccatta tagttgacag aatagccatc tacggtcgta ttcactgcca aagcacctga
    13081 gctataagca tagtaccagt tgccattgac ctggaaccaa cctgtcttca tgtctccatt
    13141 acctgcattt aggtagtacc aagttgaacc atcttgatac caaccagttg ccatagctcc
    13201 tgatgaacgg agatagtacc atttgttccc aaggttttgc caacctgttt tcatatcgcc
    13261 atttggctgg tctaaataat accaagtggt accttcctga taccagccag tggccattgc
    13321 tcctgaggaa cggaggtagt accatggagc aggtttgctt ctatcaatgt caatagtaac
    13381 atgaaaatct tgagtccaac ccttagcaac ttcaccaatt ggtagtggat tgtaagaatc
    13441 atcaccaaaa tttcctagct tatttaaata ataccaattg ccgtcttcat aaatccagcc
    13501 tgtctttaca gcataattat tatcaaaata ataagttctc ttttcagcag atagaggata
    13561 ttgttcaccg tatacttttc ctgtattttc agacgttttt gcctctagta cttgtttatc
    13621 tgtttgttct agcaaagcac catcttgtcc aaaatagtac cacttctcct gaaggccaat
    13681 ttcgaagact ggacgattat caaataaatc atcacgataa cctgttccag gaatttctaa
    13741 gtattgccaa ccgacaacca tctctccaat atcaccaaaa tagtaagttt taccgtctat
    13801 tttatgccaa tagattgctt tatgatcatc ttttacataa tattttctat tatccttatc
    13861 aacaaatcgg ccacctgtgg tattcgcaaa taccgtattt gtagctagca aaccaaagaa
    13921 aaagactgct agtagaactt gcatcatttt tttcaaaagt ttcatttata tatcctccta
    13981 aaacgattgt agcaaagaca cgactgcatg tcaatatata aaaactcctc aaaaaagcag
    14041 ttacacaact gcaactgctt ttctattctt gcatggtgta accaacacca cgcacggttt
    14101 taatgtagct tttttgtcct ttaacatcaa gcttgctccg tagatagcgg atatagacat
    14161 ctacgatatt tgtctcggtc gcactttcat acttccaaac actttccaac aattgctcac
    14221 gagtcaatac tttcttgctt cccataagtg tcgccaaaag gtcatactca cggcgggtca
    14281 gagcaatcat ctcttcacca cgataaaccg tgtgatgttc aacatctatc ctaagattgc
    14341 ggtaggtcct tggaactttc atcagactgc agtgttgatc aatgaagtcc cgacctcgga
    14401 agatcgccga aatacgcgct accagatttt cgataaagac tggcttatag atgtatgaaa
    14461 ctgcaaaacg ctgaacaact tccagctctt cttgcaagtc ttcccaatga tctaaaatca
    14521 tgatgactga ggcaggttta gttcgactca atttttctgc aaaatcctga gccatcatat
    14581 ctcccagatt aacgttcaat aaaatcaaat catagtctgt ctgaagagcc atggaaaggg
    14641 ctttttgccc ctcctctacc agatcaaccc gatactgctc tttctggagt tccaaactta
    14701 aaaaatgagc tagatttcgt tctttctcaa gtaataaaat ccgtttcccc atggctgacc
    14761 tacttatttt tcgtcatacc aagagtagtg gaaggttcct tctttgtctt tacgttggta
    14821 agtgtgagca ccaaagtagt cacgttgtgc ttggatcaag ttagccggaa ggtcagctga
    14881 acggtagcta tcaaagtaag taatagctgc tgagaaagtt ggcactggca caccagcttg
    14941 aaccgcaaga gctacgatat cacgcactgc ttgttgatac ttagcagtaa catccaagaa
    15001 gtactcgtcc aaaagaaggt tggcaagatc tgcatcgcgg ttgtaagcat ctgtaatctt
    15061 ttgcaagaaa cgagaacgga tgatacagcc atcacgccag atagatgcga tatctgcaaa
    15121 tggcaagttc cagttgtttt ctttagaggc tacacgcaat tgagcaaatc cttgtgcgta
    15181 tgaaatgatt tttgagaagt aaagggcttg acggattttt tcaatcaatt cagccttgtc
    15241 tccttcaaag ttgaaggcag ctggttttgg aagcaccttg ctagcatgta cacgttcttc
    15301 tttgtaagtt gaaatgtagc gtgcaaacac tgactcagta atcagtgaca atggtacacc
    15361 aaggtcaaga gatgattggc tcgtccattt accagttccc ttgttacctg cagcatcaag
    15421 gatgtagtct acgattggtc catcttggtc ttcatcgtct ttacggctca agatatcagc
    15481 tgtgatttcg atcaagtagc tatccaattc acccttgttc cactcagtaa agatttcagc
    15541 catatcctct gcagaaaggc ctagcaagtg ttgcatcaag tcatagcttt ctgcgatcaa
    15601 ttgcatatca ccgtactcga taccgttgtg aaccattttc acatagtgac cagctccatc
    15661 aggaccgata taagtcacac atggtttgcc atcttctggt gctttagctg agatttcttc
    15721 aagaacatcc gaaaccaatt cgtaggcctc tttttgtcca ccaggcatga tagaaggacc
    15781 ttcaagggca cctttttcac caccagaaac tccagtaccg ataaagttga tacctgagtt
    15841 tgccaattct tcattacgac ggatggtatc tttgtagaaa gtgtttcctc cgtcaatcaa
    15901 gatatcacct ttgtcaaggt gtggaagaag ggcttggata gtagcatctg taccaggtcc
    15961 agcttgaacc atcagcatga tacgacgagg tttttcgatt gagtttacaa aactttcaac
    16021 gtcatagctt ggtacaaaat tcttttcagg atggcaagca atcacatctt ccgttttttc
    16081 tttactacgg ttgtagatag caactgtgta accacgtgat tcaatattaa gggcaaggtt
    16141 acgacccatt acggccatac ctacgacacc aaagttagct tttgtcattt gacactcctc
    16201 ttgtttcatt tgttttattt tatcattttt acttttgaaa agaaagaatt ttgtaacgac
    16261 tgcttagtag tccaagtcat ccgcatgacc agcgccattt ccgacaaagt agcctgtctt
    16321 acagttaagg gtaaagagat aggttccatc tggcttatag aggttgtaat aaccattgcc
    16381 gttaacgata ttgacacgtt caaggatata ttggtctcca gtgatatagc cacgtgaacg
    16441 tgaagttgct agaatttgtt ccaaaacacc atccgcaaaa tcccaggcag agttattact
    16501 atcatcaata gcagactgat tataggcaac acgactggca cttctttgaa cagcaacccc
    16561 tgcactcgag agacccatat tgacttcact gcgactactt ctagtctcat ttgaactact
    16621 tggttttggc tcactagtcg tatttgaact tgcttgactt gaacttgagc tgctagtttg
    16681 acttgaactt gagctagaag tacttgtttg ctggctctta ccaagactga tagccttatc
    16741 tagcacttta tcaagctccg tatttccagt tttaatatcc gtaaatttag catccgattt
    16801 ggctttggca ttggtatcca acacaccatc cacaatagct ggtttttcaa attgtgcatt
    16861 gacatcttga atggccttga tttgcgttac tagactatca tatttagatt tggcaagcgt
    16921 atgttcacga ctaccttcca gcttatcaag taaagtcttg agttgactca gtttatcaaa
    16981 ctggctattt ttcaaagccg ttttattgct atttgtgtaa aaggcgtcat aaagtgtatt
    17041 aaaatcatcc acatccgatt gattggctgt agttgattga gaagtttcaa tttccttggt
    17101 cgaacgagcc acttgacgat agacataata agcactgaca caaataatta ctgatacaag
    17161 cgccaaaacg gtcaaaataa agcctttttt acttttcttt tctgagtcct cttgatccag
    17221 acgagaaagg ttttgctcct cttccacttc ttcagtcatt tctgttggat tttcatcatc
    17281 tagcaataga ggatccaagc cttgctcctc atcgtccaga ggcaagggta gtaagatgtc
    17341 ttcagaagat ggaacttcct ccgaagaagc ttctgacttc tctacagcct cacgcatctc
    17401 ttgaattaaa tcatccagac tctgagtttc aacgaactcc tcttttttgt attgacgagt
    17461 cgcaaactta tccgcctcaa tttcatctct gtgttgctta acatacttgt ccaaaatgga
    17521 atcctcaggc aagactcctg cttccacttc ttcattttta cgaatagctt gaccaactgt
    17581 tagctctttt gcttcatcaa aatcaaattg cggttcttga ccttcttttt tatgacgatt
    17641 tcgtcttttt ttactcatga gtatcccttt ctattttacc tcttgccgtt tgacacgctg
    17701 accaaaatat tgatatagat ccactttcaa ggttccgttg tataacttac gcttcttatc
    17761 tgcttggcta ccatacttgc tttcaaaagc ttcatcacta gtcaggataa atttgctcca
    17821 agttttcagc ggtgcaaata cttgccccat ctcagcatag agcttggtca ctcctgcatc
    17881 atctgacaaa cgttcaccat aaggcggatt ggaaatgatt actccattga ttttatcgga
    17941 acgtaaatcc tgcacgcgca tctgcttaaa agtaatatct cctgcaacac cagctgcctg
    18001 agcattggcc ttagcaattt ccaccatgcg agcatcaata tcacagccca tgatatccaa
    18061 ctcaagctta cgttctactt ttttagccgc ttctgtgcgc acttcttgaa tcaagcgatc
    18121 gctgatccag ttccattcct caaatgcaaa agagcgacga agacctggcg ccatctttct
    18181 agcaatcata actgcctcaa tacagaaagt ccccgaacca caggtcggat caatcaaagg
    18241 cttgtctgga taccagttag aaagttgtaa aatggctgct gccatatttt ccttgatagg
    18301 agcgccacct ttttcggtac gataaccacg tttaaagagg ctagacccgg tcgtatcaat
    18361 catgacagtt gccacatctt tgagaataga gacctcaatc ttaaactctg ggccattctc
    18421 catcagagga accccttctg ggcgagcata gtgtttctgc aatttcttga caacagcttt
    18481 cttagaaata gcctgaacac tgggctcatt gtgaagtttg gacttaacac atttagcttt
    18541 tgaaatcggt aaccgagctc caagtggtaa ataattttcc caatctaaag cgaaaactcc
    18601 ctgaaatagc tcttcaaaag tcttagctgg gaacgttcct acgataattt tgatacgatc
    18661 tgctgcccga agccaaaggt tggtttcgat aatagctctc acgtctcctt gaaaacgaac
    18721 acgtccattt tcaacctgac aatcgtagcc caactctcgc acttcacgac caacgacagc
    18781 ctcaagccct gctgccacag ttgcaattaa attaaattct tttttcatgt tacagtcttg
    18841 caagaccttt ctatatgtcc actttaaaaa atgaggttga gaaaatttct cagccccaac
    18901 ctatatgcaa ttttctataa gccatgtttt gttccagaag tgactaggac cacttccttc
    18961 gataatcatc tgtctaccac taacagctct agctgatagc agtcagaata ctacgttcgt
    19021 ttccgcatac tccatgcccc gaccaaaatt tgggttgcta gcttgagggg tttaccgcgt
    19081 tccactctct ctgtttccag aaagactccg tcactgtggc actttcaagc ctactctggc
    19141 ctatccaagg acttagccat ttcaactgcc gtaacgattt ctcgtcccta ggcttatggt
    19201 ttcgcctagc acaaacacta caggcatcac agcctgtgct agcatggact ttcctcatga
    19261 gaagcaattc tcctcacgcg attatccaaa aattgcacat ctcaaataac tacttaaaaa
    19321 tctgagttat ctaaaatttg tttaccaaaa acttcttttt ccaatctatt caggcgtttc
    19381 aaaatatcaa aattcgtcat agaacttgta attgccgctt caaggggttc tgcttgaact
    19441 ggtgaaggtt tcggtttacg agttaattct tccttcaaat ccgcaatttc ctgacgaagt
    19501 gacttgacca aggcagcata ggtttcatag tccttgatga catcgtctaa aaactcgtca
    19561 acttctactt tattatagcc acggacttca cgtccaaact cttgttcaaa aatatctttc
    19621 gctgaaaaaa taatacttgc catgtctctc tccattctcg cttgctagta ttattatata
    19681 aaaaaaggca aacaaaaatc aaggtcaaag cttcaatttt cggaaaaatt ttcagcaagt
    19741 tcatttaatt gatcaaatgt taatctcttt ataaaatagt cctcttgatt tttcatcttt
    19801 tggtaaaaat aagctagttt tgtttcattt tcttcatcat aaaaaagata ggaactagtc
    19861 gtgttttcca gcaaaaactg ctggtaatct cttaactgcc ccttgtgttc atagcgagga
    19921 taagcatatt tgacaaagtc tacctgctta aagcgactca gtttcatctg attgccttca
    19981 ttccaatttt ccccatgggt ttcaaaagca aaaatggtgg ccaactggaa accgtattcg
    20041 gttttcatct cctgagcaac ctctaaaacc caatattcaa aacccaaact ccctgtaaac
    20101 acaagccaag acaccccatc tgctgccata gcctctaaat ctttacgtat cgctttttta
    20161 atcaatttaa gacgaggatc cttatcagaa aataaaccca aatcaaaagc agaatagcct
    20221 aaaaccaaag ctgtagccat ttttaaccct ttctgtgcaa attatggtat aataaagtga
    20281 tgttattgta ccaataagga gaattatgat caactatcca cataaagttt catcacaaaa
    20341 aagacaaaca tctctttctc aacccaaaaa tttcgcaaat cgaggaatgt cttttgaaaa
    20401 gatgatcaat gctaccaacg actactattt gtctcagggc ttgactgtta tacataagaa
    20461 accaactcct attcaaatcg tacaagtgga ctatccacaa cgaagtcgtg ccaagattgt
    20521 tgaagcctat tttcgacaag cttcaacgac ggactattct ggcgtttata atggatatta
    20581 catcgacttt gaagtcaagg aaacaaaaca aaaacgtgcg attccgatga aaaattttca
    20641 tccacatcag attcagcata tggaacaagt ccttgcccaa caaggaatct gctttgtcct
    20701 tcttcacttt tcttctcagc aagaaaccta cttattgccg gcattcgatt tgattcgctt
    20761 ctatcatcaa gataagggac aaaaatcaat gccacttgaa tatattcgag aatatggata
    20821 tgaaatcaag gctggtgcct tccctcaaat tccttatctc aatgttatca aagaacattt
    20881 attaggtggt aaaacaagat gaacaaacca acgattctgc gcctaatcaa gtatctgagc
    20941 attagcttct taagcttggt tatcgcagcc attgtcttag gcggaggagt ttttttctac
    21001 tacgttagca aggctcctag cctatccgag agtaaactag ttgcaacaac ttctagtaaa
    21061 atctacgaca ataaaaatca actcattgct gacttgggtt ctgaacgccg cgtcaatgcc
    21121 caagctaatg atattcccac agatttggtt aaagcaatcg tttctatcga agaccatcgc
    21181 ttcttcgacc acagggggat tgataccatc cgtatcctgg gagctttctt gcgcaatctg
    21241 caaagcaatt ccctccaagg tggatcaact ctcactcaac agttgattaa gttgacttac
    21301 ttttcaactt cgacttccga ccagactatt tctcgtaagg ctcaggaagc ttggttagcg
    21361 attcagttag aacaaaaagc aaccaagcaa gaaatcttga cctactatat aaataaggtc
    21421 tacatgtcta atgggaacta tggaatgcag acagcagctc aaaactacta tggtaaagac
    21481 ctcaataatt taagtttacc tcagttagcc ttgctggctg gaatgcctca ggcaccaaac
    21541 caatatgacc cctattcaca tccagaagca gcccaagacc gccgaaactt ggtcttatct
    21601 gaaatgaaaa atcaaggcta catctctgct gaacagtatg agaaagcagt caatacacca
    21661 attactgatg gactacaaag tctcaaatca gcaagtaatt accctgctta catggataat
    21721 tacctcaagg aagtcatcaa tcaagttgaa gaagaaacag gctataacct gctcacaact
    21781 gggatggatg tctacacaaa tgtagaccaa gaagctcaaa aacatctgtg ggatatttac
    21841 aatacagacg aatacgttgc ctatccagac gatgaattgc aagtcgcttc taccattgtt
    21901 gatgtttcta acggtaaagt cattgcccag ctaggagcac gccatcagtc aagtaatgtt
    21961 tccttcggaa ttaaccaagc agtagaaaca aaccgcgact ggggatcaac tatgaaaccg
    22021 atcacagact atgctcctgc cttggagtac ggtatctacg attcaactgc tactatcgtt
    22081 cacgatgagc cctataacta ccctgggaca aatacccctg tttataactg ggataggggc
    22141 tactttggca acatcacctt gcaatacgcc ctgcaacaat cgcgaaacgt cccagccgtg
    22201 gaaactctaa acaaggtcgg actcaaccgc gccaagactt tcctaaatgg tctcggaatc
    22261 gactacccaa gtattcacta ctcaaatgcc atttcaagta acacaaccga atcagacaaa
    22321 aaatatggag caagtagtga aaagatggct gctgcttacg ctgcctttgc aaatggtgga
    22381 acttactata aaccaatgta tatccataaa gtcgtcttta gtgatgggag tgaaaaagag
    22441 ttctctaatg tcggaactcg tgccatgaag gaaacgacag cctatatgat gaccgacatg
    22501 atgaaaacag tcttgactta tggaactgga cgaaatgcct atcttgcttg gctccctcag
    22561 gctggtaaaa caggaacctc taactataca gacgaggaaa ttgaaaacca catcaagacc
    22621 tctcaatttg tagcacctga tgaactattt gctggctata cgcgtaaata ttcaatggct
    22681 gtatggacag gctattctaa ccgtctgaca ccacttgtag gcaatggcct tacggtcgct
    22741 gccaaagttt accgctctat gatgacctac ctgtctgaag gaagcaatcc agaggattgg
    22801 aatataccag aggggctcta cagaaatgga gaattcgtat ttaaaaatgg tgctcgttct
    22861 acgtggagct cacctgctcc acaacaaccc ccatcaactg aaagttcaag ctcatcatca
    22921 gatagttcaa cttcacagtc tagctcaacc actccaagca caaataatag tacgactacc
    22981 aatcctaaca ataatacgca acaatcaaat acaacccctg atcaacaaaa tcagaatcct
    23041 caaccagcac aaccataaca tttatcaccc agatttttct gggtgatttt gtcatagcac
    23101 tattgtattt atgtgcttaa caaaagcctg agaagatgtc ttctcaggct ttttctgtac
    23161 tataaacctc attggaaaag atagagactg cttccgtaat cttgttaaga ggttttacta
    23221 aatactagtc tttcttcgtt tttactacaa agagagcaga tagggctaga ctaacacttg
    23281 ctaggaagag ggctgtgtca gattgactct cacctgttgc tggcaagatt tgttcaggtg
    23341 tttctggtct agctatttct tgcttataat ctggcgtctt accttgatcg gaaactaaag
    23401 agctaagttt aaactcaggt ttctctacag ttggagcatc tggtttcgta gcagaatctt
    23461 ttaattggtt gagataatct gcaaagtatt ccaccattct ttccgtcaag agatggttat
    23521 ccgtcgcata tttcatgctt gcctgaacgc tagctacttc ctcttgattg tctttgtctg
    23581 ttaatttctg agctttagcc aaagctgctt catagcctga caagtcaaga ggtgtttctg
    23641 ctacctgtgg acgagtaaag atgagttccg ctgcagattg gtatttatcg ccaccatctc
    23701 cgtaagtctt agtacctgta aggacaattt tcttagcctt aattgtctta ccaaaatcaa
    23761 tgtcttttgg cttattgtta tctggccaat cagttgcagt aaaggtatgc tccttgccag
    23821 actcatctgt cacaacaagt ttcacatcac gcaagttacc atttgaacct gatccacgtg
    23881 gaacgtaacg aagtccagtg atttcagttg gttctttcaa gaccatggtt gcaggcttgc
    23941 ctacatctcc accattccaa gatgtatgcc ataaactaga taagtttcca tcaaaggcat
    24001 ttgcaagacc ttcttgagcc tgagcaggag ctgtaagact tgcaaagtca tctgctacca
    24061 aagccgtttt cttctgaacc aaagcattct tcaaggcttc aatcttggca atctctgcac
    24121 gcgcttcttc cacactgata tcatcatcgg cctgactaag gttaaagacc gcctctttca
    24181 aagcatccat agactctttg gtgtagttag tcatggcaac cgttggcaag tagttcttca
    24241 gagcattttc tgtcaacatc ttacctgtta gggtaatttc ttcgatttga agattatcca
    24301 tcatgaagtc gttataacca cggaagttgg catttccacc agaatcacca cgagtattac
    24361 ttgcattgcc agttgagtag atacctaccc aagtatcccc tgtttctgca cctgtcacaa
    24421 ggaaggttgc cttcttggct ttcttagaat ctgtccaagt atttggcaat tcatgcattt
    24481 ccaagttgct tgcttgagtg ccacgacgac ctgactggaa ttctccctta ccgactacaa
    24541 aagcataggt attgtctgaa cctgcttcgt attcaaaggt tacacggtag gtcttacctg
    24601 cttcaaaacg gaagttttgt gggatggttt ggtaaaccaa gttacgacgg ctcactagtc
    24661 catttgtctt gagtgaccaa tttccttcga taacatcatc gactttctta ccgttccaac
    24721 cacgttgtgt atatggatcg tgtttttcag acaagtgagt gcggttgtct tcgacacctt
    24781 cgacaccacc cactacaaat gggaagattc cttgagctac attttcaaag tcttgcttga
    24841 aggttccttg acctgtatcg tgcttgtctc catacatgct tgaattgttt tcaaaggtac
    24901 gaatttcatc aaagtaagtt gcttgatcac cagcttcacg actcaatgtc agagtaacat
    24961 ttgagacgtc cgatcctgtt gtaaagaagg cgtacatgtt ttggaagtaa cttgtatcgt
    25021 taactgtagc attgttacga cgtgtattgt gggcgtaggc ttttacatag ttgagggcga
    25081 gagacttatt ggtataagta gtcacttctt tttcaccagt attcacagtg atacttgcct
    25141 tggcattact acggttatcg acaccgacat aaacggcata cttggtattt ggtttcaagc
    25201 cagttaattt ctgagtgaga ctaacttttt ctttgtttcc ttgaatacga agcatatcgt
    25261 ttgccccttg agacttgaca atttctgcct tagaagcatc gcctgaaatg gtccaatgtt
    25321 tcaaggtacc actgttaaat ccttggtcat agatgtgcat gccttcactc catgacattt
    25381 caggattggt ttgttttgaa cgatagagaa cgtatggttg atttgctaga agatctaggg
    25441 taattttacc atcttttaca gttagttctt gctcttctgt cttaccttgg tcagttagct
    25501 tgtaaaggta aaccttgctc tttgcccaat cgcttggaag ggtccaagtt gttgcaccgg
    25561 cctgcgtatt gaagtagtac atcttttcct tatcagtaga aagtttctta ccatttgcat
    25621 cccagttcca aggagtcaag taagctgaac catcttggat gacacgtccg ttgagagtta
    25681 ctgtacgttc gcgatattgt ggactattga catcatttga cttacgagtt acaactactt
    25741 tattattgtc agcatctacc aattccactc gcatttctgg agtccattta taggtactac
    25801 cgttatcggt catagtcacc ggtgtaccat tttcccattt acttacagtg aagtgttgga
    25861 agtacttagt catgacgtca tgggcaaata agttggttac atagccattg tagtcacttc
    25921 ttccctgcca gccttcaaag tctttcatgc tgtagccacc tagcagtgga tagttggctg
    25981 caccaccata acttctgtag tccccaaccc aagcatcttt ttggtggtta cggataaagc
    26041 gggtgatggc actgttgata cctttattgg tgtagccacc gtaggtcaag tcagctgccc
    26101 agtgatggaa ggtagagtcg tactcaccac catggcccca ctcgatcgca aagcgccagc
    26161 cttgtttgtt aatttcttta gcaagaacgt gggtagccca ggcaccgtta tcacctgatt
    26221 gaccattacc ccaaacgtcc acatagataa agtcgagacc gtcaccaagt tttttcttca
    26281 aatcttccca acgtgccaaa cgaccatgag ctaggtcata ggcagcatca atgttgatac
    26341 cttgatctag ccagttccaa ccatagctat agcttccatc tggattctta cggagaattt
    26401 tttcattgaa gtatttagac tcaggataag tttctgaagc gttaacgtgg atacctagat
    26461 gagctccata tttcttagcc ttctcaatta gggtcttgaa gtcttcgaca ccaccgatac
    26521 gcttaccaat atcagcatag ttcaagtgac cagagtcatg gccttcgcta ccatatcctt
    26581 taaggagaac accttgccca agaccatctg tgtggagatt gattttcttg ataccatcca
    26641 aggtcataag gaatggattt tgtgcttgag aaccaaagtt catcgcgata cggtaagctg
    26701 tgatatcctt aactttttcc caaccttgag gattgttcat aatgctacga taagcaatgg
    26761 caccatcttg ccaatcgact ttcttgtctg cattggcatc ttcagtgata acaaccttag
    26821 cacttggaag ttccttcgtg tattctggga aaacaatgcc cttataagct ttttcccatt
    26881 gccattcaga gctgtggatt cctacatagt tggcatttcc gactgtttct ttataagctg
    26941 tcaaacgagt ccagtcattc gaaccaccac catagctatt ttgagagtta ctccaaacac
    27001 cagcagcaag cttatctgta gaaacaaatc catacatgta acccttggct aggtctttca
    27061 ttggattggt tacatcgata tgatcatctc cgctgacatg cgtattgttt gacatggttg
    27121 ccccatcaaa cttagcacca gtttgatcac tagaaacaga gactaaagca ttgccgagga
    27181 aactaataga agaaagtagt tttctttcgt catcaatctt ttgacctgga gtgacttgat
    27241 tgtggttgac aatcttggtc acatcaaagt gcaattgatt gtccacaact tgcaagcgta
    27301 ctgtcatttc cgcattgatt aagtgagcat catcgcgaag cttcatcaag tactctgctg
    27361 ttgtctcatt gattttctta taagtgactt caggggtgat tcggtggtta ttgataaaga
    27421 cttggttgaa ctgttgcacc tgtcctggca aagtatgtcc attcaagctg tattccttga
    27481 cacgagggaa ggcttggtca atcactgctt tgaggacctt agactggatc gtatcataag
    27541 tcaccttgct atcgtcaaca accggacctg tttctttctg ggcaggagta tcatccgctt
    27601 ttacgccttc ttgattgtct gttttaacgc tgacaactgt acggtcattg ccataagttc
    27661 ctgccttgag aaggatcttc ttctcatttt taagattatc attgaccgcc ccaggcagtg
    27721 tcactgtgtc aaagagattg acatcgttat tgctggcatt gagttgtcca tctgacttga
    27781 gagtgataga aagacggttt acagaacccg gttctggtgc aactacacgg ttgcctttat
    27841 accatgtgct attacctgga gttttgtatt cccagaacca gccatccttg tcataaccga
    27901 caaaaacatt attattggta tctttaaatt tcaggaagac accaaagcgt gatttgccct
    27961 tttcagaatc ttctttgaag gttaaatcaa cagttgcatt tccattggca tcaacggtca
    28021 agcccttctt ttcaaacagg gctggtttgc ctgcgttatc attttgagca gttgagaata
    28081 gttggttgta gcggacacct ttttcttctc ggatagtgac tgttccctgt tgttcttttt
    28141 tctctaccgt ttgccattca ggagttaccg tcttaggtgt ttcaggttta gcagctgcag
    28201 gtttatcctc ttttggtttt tcttcagccg gtttttcttt ttcaagtgct aatagttctt
    28261 cttctgtctt aggtccatct gtaacttctg gagaaccttg gtcttctccc accttaggcg
    28321 cttcaaaatc acgcccatca ttctcgtttg ctgttgcaag agcagtagct aaatcagctg
    28381 gtaagttcgc cgtggaacca gactgcacgc tatctgcaag aaccggactt gtcccaaaga
    28441 atgcagctcc aatcataaca gaagcaacac ctaatgaaaa tttccgaata ctatatttac
    28501 aacgtttttc aaataatcct ttattcataa acttctaaat cctttcattt tttagcaagc
    28561 gtttacattt cctttttaaa aaatactcct tcctctgaaa gtgcaatgct taaccctgat
    28621 aaataaaaaa taattgtact tttaatataa caaataatag ctgagaaagc aataggaaac
    28681 aagtaaaatt aacaaaaatt ttctctattc tgacaaaaag aggctccaac ttttgggggt
    28741 cagtacattt agaatctttc tttacaaatg taaaaaagga ttcagcattc aagagaaata
    28801 ctaaatcctt tcttatattt tgcaacagtt atttcacatg ttttgcgaga tcttcttgag
    28861 cctttttatt agactcttct ttttctttca tccatttttc ctgagctttt tggtattcat
    28921 ctacagtgac tgccttgtct tgaagttcca agtatttata caagactggt tcacttgtac
    28981 ctttatttcc tgacaatgca aatggtattg taaatggtac catcttagac aagattgggc
    29041 gccctgtacg agatgtagtt ggaataatca aagcactatc tgtcaaccaa gcttgggctg
    29101 cagcgtattt atcatagcgt ttagcaacat ctgtagcctc atcaccagcc tcagtgacca
    29161 atttttcgta gtcatataga cctacttttt tagcagctac attatcttcc cctgagtcaa
    29221 accctaaata tgttttagta ctttctccta cagaaggttt gataatatca aggtaggttg
    29281 atggatcggc aaagtctgga ccccaaccga cattatctga taaatcccag tcttcgccag
    29341 cagcattttc agcaaaatat gtaatattgt ttacttcgtc tttttgtagt tgttggatat
    29401 caataatgac attatcagct cctaaagttg cttccaagga ttgtttcata gattggacgc
    29461 gctgaacttt tgtagttgct gtctggtcaa ctggcatatc caaatgaatt gggaaagtca
    29521 caccttctgc ttgtaaggct gatttagctt tagcaaattc agccttggct ttttctggat
    29581 tgtaaagacc atcctgagaa tctgcaagat taacatcctt ccattcatcc ccataagtga
    29641 ccaatttctc tttgaccata tcgccaaagt ttttaccatc tgcttgaaca aatgttggtg
    29701 gaacaaagat attacgtaag attttgcttg ctccagtttg tccattcaac tgagaggcat
    29761 aggctgtacg atcaaaacca aaggcaatag cctgacggaa atccttgttt aagagagcct
    29821 ttttagtcga tgccttttgt tcgtcgctgg tcttagatgt atatttatag gactgacggt
    29881 caatatttgt accaactaga tacgtaatag agccttgttg agtatagaca atattgtcct
    29941 tcatactctt ctcaagctct gcgaaacttg cacttgttgg atagagacga gctgctgtaa
    30001 ggctaccatc tttaaagttt tctgcaggtt tgctggtatc ttgaccatcc cagaatgaca
    30061 atttaacttt gtcaacatgc acattgtcct tatcccagta gttcggattt ttcgcaaatt
    30121 caacagagga ttttgtcaca atggatttca acaaataagg accgttatac aagagactac
    30181 ttggatccgt cgctttggca aaatcatccc cttttgagtt caaaaactct tcattaactg
    30241 gcgcaagcac acccatggtt gtcttagaat tccagaagct ttctggtttg ttcaaagtgt
    30301 actgaactgt ctgatcatcc aaagccttaa ttcctacttg tgagaaatct ttgatttccc
    30361 cttttacata ggcatccaac cctttgattg attcttgaac aaggtaaaga gcatctgatt
    30421 ttttatcagc agcatatttt agtcctgtta caaagtcttg agctttgact gccgcgtatt
    30481 cttcaccttc agaagtatac cattttgcat ccttacggat agtataagtg taagtcaatc
    30541 catccttgga tacagaccaa tcctcagcca tagacggcac aaagttcccg tagcgatcat
    30601 tttctagcaa accatcaacc acgttactgg taatatttgc tgtcgcagcc ttagcagttg
    30661 tcaaatagtt gaggttatca gggtctgtct cataaatgta tgagaatgtc ttctcacctt
    30721 tagtgcttga acctgatcca gagcatgcag ctaaagtagt cgccgccaat aatgtcacgc
    30781 ccgcaagggc aagtagtttt gaacttttca tccttttctc ctttaaaact ttctctccat
    30841 tatagactct tttcagaaag ttgtcaacag aattttcaga atttttgaaa attatttttc
    30901 aaataacatc tttgcaaaaa atatgaatat cgtaaccgcc caataacgaa gtctattgaa
    30961 aaatctccag actagagaac tcacggatag ttcctaatct ggagatttct tatttgcact
    31021 tttcttgtac aactttagtc
//
LOCUS       DAVBCW010000013        31003 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVBCW010000013 DAVBCW010000000
VERSION     DAVBCW010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31003
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.013"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(297..515)
                     /locus_tag="VQ514_000509"
     CDS             complement(297..515)
                     /locus_tag="VQ514_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001207345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2499316.1"
                     /translation="MVLNYKPLWIQLAKKGLKKTDVIAMAGLTTNVMAQMGKDKPITF
                     KNLERICKALSCTPNDIISFEDNFSDEE"
     gene            complement(761..1039)
                     /locus_tag="VQ514_000510"
     CDS             complement(761..1039)
                     /locus_tag="VQ514_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499317.1"
                     /translation="MKKDKEVYDKVCEYLSPHLNKDMEMLELACWFRVIIVIEANSYV
                     NIRSSRLLPKFKTQKINSWCAAYSIHFNNGYCKQHTNEFKDSLKSLIL"
     gene            complement(1253..2884)
                     /gene="rlmD"
                     /locus_tag="VQ514_000511"
     CDS             complement(1253..2884)
                     /gene="rlmD"
                     /locus_tag="VQ514_000511"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HET2499318.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSLHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWCTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSQKNASLNKITNAHYVCDTAENAMKKWLKEGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(2958..3932)
                     /locus_tag="VQ514_000512"
     CDS             complement(2958..3932)
                     /locus_tag="VQ514_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HET2499319.1"
                     /translation="MRKKLFLTSAAVLWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGMEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDVRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(4006..4620)
                     /gene="pmp23"
                     /locus_tag="VQ514_000513"
     CDS             complement(4006..4620)
                     /gene="pmp23"
                     /locus_tag="VQ514_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HET2499320.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNTTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            complement(4620..5594)
                     /locus_tag="VQ514_000514"
     CDS             complement(4620..5594)
                     /locus_tag="VQ514_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HET2499321.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSRKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQKENGTYSILIKNIEDIQSK"
     gene            complement(5602..6858)
                     /locus_tag="VQ514_000515"
     CDS             complement(5602..6858)
                     /locus_tag="VQ514_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET2499322.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYEE"
     gene            complement(6920..7351)
                     /locus_tag="VQ514_000516"
     CDS             complement(6920..7351)
                     /locus_tag="VQ514_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2499323.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEQEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            complement(7353..7955)
                     /locus_tag="VQ514_000517"
     CDS             complement(7353..7955)
                     /locus_tag="VQ514_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HET2499324.1"
                     /translation="MMDRIRQELENGGAVVLPTETVYGLFAKALDEKAVDHVYQLKRR
                     PRDKALNLNIASFEDILHFSKNQPTYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(7939..8778)
                     /gene="prmC"
                     /locus_tag="VQ514_000518"
     CDS             complement(7939..8778)
                     /gene="prmC"
                     /locus_tag="VQ514_000518"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HET2499325.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASDQNLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKKVRTLKDQFGQNRMVVVDD
                     GQD"
     gene            complement(8778..9857)
                     /gene="prfA"
                     /locus_tag="VQ514_000519"
     CDS             complement(8778..9857)
                     /gene="prfA"
                     /locus_tag="VQ514_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HET2499326.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(9854..10444)
                     /locus_tag="VQ514_000520"
     CDS             complement(9854..10444)
                     /locus_tag="VQ514_000520"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HET2499327.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRGLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPEINKENEEK"
     gene            10567..10749
                     /locus_tag="VQ514_000521"
     CDS             10567..10749
                     /locus_tag="VQ514_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HET2499328.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            10886..12259
                     /gene="mnmE"
                     /locus_tag="VQ514_000522"
     CDS             10886..12259
                     /gene="mnmE"
                     /locus_tag="VQ514_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HET2499329.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNIRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTKLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEANLVLLVLNASEPLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLIEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(12306..13241)
                     /gene="dapA"
                     /locus_tag="VQ514_000523"
     CDS             complement(12306..13241)
                     /gene="dapA"
                     /locus_tag="VQ514_000523"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HET2499330.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKTIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(13297..14373)
                     /locus_tag="VQ514_000524"
     CDS             complement(13297..14373)
                     /locus_tag="VQ514_000524"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HET2499331.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGSSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPCDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(14715..14787)
                     /locus_tag="VQ514_000525"
     tRNA            complement(14715..14787)
                     /locus_tag="VQ514_000525"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14752..14754),aa:Thr,seq:ggt)
     gene            complement(14887..15396)
                     /locus_tag="VQ514_000526"
     CDS             complement(14887..15396)
                     /locus_tag="VQ514_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HET2499332.1"
                     /translation="MKKLTIRDIADIAIVTAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(15394..15490)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            15519..15950
                     /locus_tag="VQ514_000527"
     CDS             15519..15950
                     /locus_tag="VQ514_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HET2499333.1"
                     /translation="MKIPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKAYIFRK"
     gene            16032..16409
                     /gene="mscL"
                     /locus_tag="VQ514_000528"
     CDS             16032..16409
                     /gene="mscL"
                     /locus_tag="VQ514_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HET2499334.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(16451..17545)
                     /gene="hemH"
                     /locus_tag="VQ514_000529"
     CDS             complement(16451..17545)
                     /gene="hemH"
                     /locus_tag="VQ514_000529"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HET2499335.1"
                     /translation="MKKAILMMTFGSPEEISFEGVAEFFTNIRRGVRPQDHEIQTLYD
                     NYIRIGGTPLQRITREEVNLVKERLGEEYGIYFANKFSRPFIPDVIKQMETDGVEECI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEVLLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            complement(17623..18455)
                     /gene="srtG2"
                     /locus_tag="VQ514_000530"
                     /pseudo
     CDS             complement(17623..18455)
                     /gene="srtG2"
                     /locus_tag="VQ514_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218039.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system class B sortase SrtG2"
     gene            complement(18533..19531)
                     /gene="srtG1"
                     /locus_tag="VQ514_000531"
     CDS             complement(18533..19531)
                     /gene="srtG1"
                     /locus_tag="VQ514_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000851047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system class B sortase SrtG1"
                     /protein_id="HET2499336.1"
                     /translation="MKTKREKPKKSLSRRLVLAVDGVINHLLLIFAALIFLFGFYALW
                     DSNQVYSLASSSEYEAYRPVTTQQDELASFSGFSKLQELNPEVLGWINVYGTNIDYPL
                     VQAKDNEKYLNKDSKGEFAATGAIFLDARNNPKFEDFNTIIYGHHVENGVMFGDVAKF
                     ADQEFFDQHRYGSIYYNGVEKGLEIFEMLEVDAYDFNIYDPGIQGEDRQQAYLDHLLS
                     VAMHKRDISLSPSDRIILLSTCFLDVTNGRHIVVAKITDTVPKNTFHTKKSKPFPYSV
                     FDDSSLGRFLSSIPLWIWYLILFVLFLLLIFLLLVLYLILRRRRESKKNARSRPFY"
     gene            complement(19549..20745)
                     /gene="pitB"
                     /locus_tag="VQ514_000532"
     CDS             complement(19549..20745)
                     /gene="pitB"
                     /locus_tag="VQ514_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus major pilin PitB"
                     /protein_id="HET2499337.1"
                     /translation="MKKTFFKKLFTASIAAITALSVFRGVPTFADDNSAITKANGENN
                     AVVKINKTLNIAEGITTPTATFTFKFTEKTGQSSNGAPYQTGVAIPDRNVEYNKNDHP
                     TADKIQKATEDIFSGVAYGHAGEYVYDVAEAKTGWQAITKNGKTIDAMRYDKRTYEMH
                     VIVKNKVNGGVYISSVYFKENNKSNAPKVEPSEQGVYNLFDNTYTKDASKEPNPDDPS
                     QVDPNAKALTITKKVDGASGDKTRDFQFHIKIQLPSTNKTAETPVTNIIVKHGSKSEV
                     LAVVTPADTVEYNFTLKDGETFTVEQLPAGSKYTVTETGVAGYTDSSIYTTNGAEQTS
                     QGQKNVDFTLTDILIGEKKNDNKVTNKIDDVTPTGLLIDNLPFILMIGLGLAGFVVLS
                     KKRREA"
     gene            complement(20790..21341)
                     /gene="sipA"
                     /locus_tag="VQ514_000533"
     CDS             complement(20790..21341)
                     /gene="sipA"
                     /locus_tag="VQ514_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system signal peptidase SipA"
                     /protein_id="HET2499338.1"
                     /translation="MLLKKKHKKPVTQVNRDKSPPSVWGDILYLVSKLLMVGFVLAIL
                     YFFVFGLLRYNDDGMKPALKDGDLVVYYRLDKRYSIGDLLVYSYKGKERVARVIATEG
                     STIDINENGLIINGSPQQEQDIYKETLLYKEGATFPMKVPAGQLFVLGDNRTTAVDSR
                     AFGTIPIQDTQGKVVTVIRRRGF"
     gene            complement(21341..23293)
                     /locus_tag="VQ514_000534"
     CDS             complement(21341..23293)
                     /locus_tag="VQ514_000534"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET2499339.1"
                     /translation="MDAGIGTGTNINAGLTEAQRLLQSARAGAKKVVILLSDGEANMY
                     YESNSGRTIYNYYSNPNVGRMIDTPYWFTSGLERGMLNISSLIAPKIDGFYSIKFRYI
                     GSNDSITSLKGYISGYNSGIPNEIFSANNENDLQQKFKEITDKILPLGVHHVTISDVL
                     SKYVQLLPGDASHLRVVKIKDGNEQELNDNQVTIETKKNEQGLVEVTAKFNPSYTLED
                     DAKYVLKFTVTSSQEAFDAIAGDKTLTSDDAEEADATKLYSNKGAKVAYSYGIGTSRT
                     KIKDYSEKPTFKPSDPLTVPVEIEWKGVDGKSNPSANRPPSVELNLNQKKDGSIKDSY
                     RKVTSPVQTNSFTENTSFAKVAKGYDYELKAPDAPGYTVEVQKTGTKEKPSFKVIYRQ
                     LPSLTVKKILEGEQSPNKSFTINVTFSDKDGKPINGKFGNTTVTNGKAQISLKNSQET
                     ALSYLPRDTHYKVEEVENSRTGYHVTYEKQEGTLSEDVQTIVTNHRLPTLSVTKKVTG
                     AFANLLQSFKITINVKDAQNKPLNGSYSAIVNNQKTTLQFTNGKATVDLKKDKTIKIL
                     DLPLNARYSIEEEASSSRGYQVSYDKKEGTLDANKSATVTNNKNSVPETGIDFLSSTL
                     VLGVVLPLGGIFFIILLGHLVVNRRK"
     gene            complement(23423..23926)
                     /locus_tag="VQ514_000535"
                     /pseudo
     CDS             complement(23423..23926)
                     /locus_tag="VQ514_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102795.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
     gene            24285..25508
                     /gene="pepT"
                     /locus_tag="VQ514_000536"
     CDS             24285..25508
                     /gene="pepT"
                     /locus_tag="VQ514_000536"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HET2499340.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPRQTLITTDGTTLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEV
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSDRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(25544..26173)
                     /locus_tag="VQ514_000537"
     CDS             complement(25544..26173)
                     /locus_tag="VQ514_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499341.1"
                     /translation="MNKANQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDSQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSANSKDKTALLESTKAILVLNHQIQSKVSASEETSPARNAEANSDNTSAENQP
                     NATAESNIETASDENKPSNTRDSKPAESTSENETTESSTTTGNQEKPVE"
     gene            26138..26359
                     /locus_tag="VQ514_000538"
     CDS             26138..26359
                     /locus_tag="VQ514_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499342.1"
                     /translation="MDLFQELICLVQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            complement(26630..26978)
                     /locus_tag="VQ514_000539"
                     /pseudo
     CDS             complement(26630..26978)
                     /locus_tag="VQ514_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669441.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(27083..27643)
                     /locus_tag="VQ514_000540"
                     /pseudo
     CDS             complement(27083..27643)
                     /locus_tag="VQ514_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669441.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(27869..30988)
                     /gene="phtE"
                     /locus_tag="VQ514_000541"
     CDS             complement(27869..30988)
                     /gene="phtE"
                     /locus_tag="VQ514_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HET2499343.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQISQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
BASE COUNT         8835 a         6359 c         5685 g        10124 t
ORIGIN      
        1 aaaggatcta gtttttattt ttagttaaat tatgtctata tttttcatta actcatcaat
       61 aaaaatttgt ttgctaatat ctctttcttc tgattttgtt tcaaggatta ttgctacatc
      121 attcatattt ttaggtaaat accaaccatc tggcttatct gaatatccct tgaagcctaa
      181 ttgattaaaa gtagttattt gaccagtacc ttgattgatg ttttcttcaa cttcattaaa
      241 gcctatactt ctcttgcata atccctaact tgatcttctg tccttaaagt cattttctat
      301 tcctcgtcac taaaattatc ttcaaaacta ataatatcat taggagtgca agataaagcc
      361 ttacatattc tttctaaatt cttaaatgta attggtttat cctttcccat ttgtgccata
      421 acatttgttg taagtcctgc catagctatt acatctgttt tctttagtcc tttttttgct
      481 aactgtatcc ataatggttt atagttaagt accataatat ccctcttttt ttcttcatta
      541 aatttattta attatagcat aaatattgat ttcattcaat cttacattga tttagtgatt
      601 tgttttagag aatttgttgt gttattccgt ccttattttg ccataaattg ttttaagtgg
      661 gacctcttat ccttaccatt tttacctcct aattttgagt ataaaaaaga cgatagagtt
      721 tttaatttct atcgtctgag atttacatat taaattttca tcacaaaata agactcttta
      781 aagaatcttt aaattcattg gtatgctgct tacaatatcc attattaaaa tggatactat
      841 aagcagcaca ccaactattt attttttgag ttttaaactt tggtagaagt cttgaactcc
      901 ttatatttac ataactattt gcctctataa ctattatgac acgaaaccaa caagcaagtt
      961 caagcatctc catatcttta ttcaaatgag gactaagata ttcacaaact ttatcataaa
     1021 cctctttatc ttttttcata aaaggggcat acaacttagc aaatttatcc taaaattttt
     1081 tgttgccttt atcaatcatt tcaacgctcc tttaaatata aaaataatcc tatcaatctt
     1141 tatattgagc ctattttcta attgatacct tgcaagaaag gaataatagc tgttcctata
     1201 ccaagtagga atgtaataaa tgcagaaagt catatactgt cattcttttc tattaaatca
     1261 ttttgaagtg tctcaaagca tccatgatgg cttcttcttt ttcaggtgta cactgtggaa
     1321 taatttgttt atccttttta gacttgttgt aatgttctcg taattctatt ccacatttct
     1381 tttttatctg tgcaatatat aatgtcgaaa cttttaattc aaatttattc caaacatatt
     1441 ctttgatttg agcatatgtt gctttgctct ccgcacttgt caaatccatc tcatccagct
     1501 caatttcaac acttatgtgc ttatcgacat cgagtttgga caaaagtgct accgtctcga
     1561 cgtgatgcgt ttgaggaaat agatccaccg gctggacttt cttcaattca tatcccaatt
     1621 cttggtagag tttgatatca cgcgccatgg ttgcgacatt gcaggagata taggcgatgc
     1681 gatcggctcc tgtttgggcg cttgctttga taaagctttc tgtcaagccc ttgcgtggag
     1741 gatcaaccaa gataacggtt ggttgaatac cttccttgag ccatttcttc atggcatttt
     1801 cagccgtgtc acagacatag tgggcattag taatcttgtt caaagaagca ttcttctggc
     1861 tattctcaac cgcttctgga atcagttcaa caccgtagac ttctttgaca tgcttggcga
     1921 ctgataaacc aatggttcca ataccagaat aagcatcaat aaccacgtca tcttttttta
     1981 actctgcaaa gtcaatggct gtttgataga gtttctccgc catttcagta ttgacttggt
     2041 aaaaggctgg gccagcgatt tggaagtcat ttcccaacat ctggtccgta atatagtctt
     2101 gaccataaag agtgcaccac tccttaccaa aaatcgcatt ggtattctgg tcgttgatat
     2161 tttgcatgac agacacaatc tctgggaact gcttgataac ttgttcaatc aattggtcaa
     2221 cacgaaaaac ttttggacga gttgtcacca aaacgaccat gatttgtcct gaatagtgac
     2281 cacgacgcac cacaagattc cgaatcaatc cagactgttc cttttcgtca taaggtttta
     2341 aatcaaaacg acggagcagg tctcgaagag ctactacgac ttggtcaatg acaggatcct
     2401 ggataaagaa atcttcaagg ggcatgaggt tgtgcgaatt cttacggaaa aatcctgttt
     2461 ccaagacacc attcactcga cgaacgggca cctgcgcctt attgcgatac ttgactggat
     2521 gttccatacc aagcgtttca gcaacttcta catctgcaat tccagcaatc ttgtagagac
     2581 tgtccttgac ttgcttggtt ttaaacttga gctgttctgg ataagaaagg tgtcctaaat
     2641 ccgcgattcc tgaacgcagg taagccaaat ctagatcttg attacggtgt agtgactgga
     2701 caaggtattt ttcaactttt ccaaagccaa tctttttatt gaccttgagg acacgcatga
     2761 gaattttttc actcggtaaa gcattctcta caaaaaagac caaaccatct accttggcaa
     2821 ctcctgcccc ttcatgggtc aaatcaacaa tttcaacttc tacaatatca tttttcttta
     2881 acattctttt ctctttctat tggccgacaa aaaagacggc aacgttacga ttaccatcca
     2941 ttataccatg aaatttctta agaaccaaaa ctcttgaaga agttgacaag ggcttgccag
     3001 agggagctta agaagttacc accgtcctct agcgccttat ccgcatcaaa gttaaggttg
     3061 atatttttaa aactgtcgcc agcttgtgat acgatgcttt gtttaaggtc atttagggtt
     3121 ttagtgaaat ctgcattgct gaggatatca ctctttgaga gattcaaggc aaaattgatg
     3181 atgatattga tctggtttcc tgttatgacc tgatcaagtt tgtaattttt taaggtatct
     3241 tcaacaatct tgcggacatc ttcttctgtc agatttccct tgctttcttt agctttggcg
     3301 agtcctgact tgatatcagc tagggcaacg tttaatttat tagcatcata gcctgatttg
     3361 tccttgtttt cagcattgat atctgacaaa gcctttagct cttcttgagc caaatcttta
     3421 ttggcttgcg gcaccttggc tccattagct tctagcgaat agtagatccc agccaaagca
     3481 ctctcacctg taactggaat aggggctgct acagtgattt tggcatgttc catacccagc
     3541 gttactgctg cgtttcggta catatcctga gtcaccttag tgatattttc tggtgtttca
     3601 atcttgacct caagtggcga tttgtcacct agcttttgaa tcttggctga tgaatacaac
     3661 tgtaagctag agtcattggc cacattcatg attttagaat aaacatcagg tgtcatggtc
     3721 ttgagttctt tggtatctgt tgaggcattg tagcccagtt ttttaagagt ttgatttttt
     3781 tggtcttcag atagggagga acctaggaca tattcaggtt ggacataggt ttcatcgata
     3841 actttttgaa catctgttgc tgcatggacg ctattcatag ctgttactgc ccacaagacc
     3901 gcagcactgg tcagaaagag tttctttctc atagggaatt tcctccttta cttttctaga
     3961 gtaatatatc tatcttaaag aaaacttata acaaaaacac ctggtctagc cagatgttga
     4021 aaagagagtg aaacatttga tgatgtaaag gttaagtcgt acctgtctag aataataata
     4081 gtttcctcca tttacataga gttcagcacc gtgaaaaatg gaaatggggt gaatataact
     4141 ataagtcttt ccagtcgtat taccaagcaa gggggcaaca gtctcacgag agtactgttt
     4201 ggctagagcc aaggtatttt ccttgccatt ttgggcgata aaatcgatat aggcaggtcc
     4261 aaaattatag gcttgaacag ctgtccagat atctaccccc ttcttctgcg ccagatagag
     4321 attgcctgtc agagtttgaa tgccttgccg aatgctagag gcattatcat tgatggtgtt
     4381 ggttgaacca cttgcagact cactagactg cataacatcg ccttcttttc cttttgtttc
     4441 agtataaatc atagcaagca caagctcttc gtttgctggg gtgtcttttt cactcaagat
     4501 ttctcgcacc atgggttgat aggtcatgac ttgtttgaca tcttgatgaa cgcggtaagc
     4561 tttatagcca gcaaaaagga agactgctag tacaagcact cttcgaattc gtttaaacat
     4621 tatttacttt ggatatcctc gatatttttg attaagatag agtaggttcc attttccttt
     4681 tggataaact caacagactc ggcgtcttga tagacgttat tgggaacgat gagctcaatt
     4741 ccatttgata aggagagttt ttggttttca aatttcttta attggcgact ggcatcaatt
     4801 tcatcaaatt gaacaggttc tggtacggct tctttgactt ggtcaataaa gctcaaacga
     4861 gccgtcagat tgttgtcaaa aaggtcatta gccaatttct caggtgacaa ttcattgctt
     4921 tcttctaggt tgttgaaaat cgctgatttg accttggatt gaaattgaaa atcatctgtg
     4981 ttaaaagatt cagcaattct ctgggctgtt ttttccagtt ccttgataga ttttttagga
     5041 gaaatcttag gagcgacagc aagaagatta tctgaaaaat agttcaaaaa agtcccgttg
     5101 tacttgattc gtttttcaat caggtgatac ttgcgactct gaagattgac caccaaggcc
     5161 tcgtcagcac ccgttccaaa tccaggcagg ttattctgag tcagcttgat tggattatca
     5221 acttctcctc cgaggtgggt caaggtctcc cgcagggcaa ttcgcaagaa agcgaaatgt
     5281 tctacacctt ctttagaaaa ttgaacaaaa atcaagtcat tggtcttgag attttcagaa
     5341 atgctaaact cctctttcca gagattagcc agcgttactg atgtctccaa caaatcgtct
     5401 gtaatatgat tgaagaaggg attttcttct tcgaaaatcc cagtcttggc ttcatctgaa
     5461 tacacatgtt caattttttt acgtaggtat tcttcgattt ttggagtaat attgagaaac
     5521 ttatctgcta agaacagctc ggtatcatcc ggactgaact ggtgaataat ggctttctta
     5581 atataaatgt ccataaaagt tttattcctc gtataatggg aaggcatctg tcaattcttt
     5641 gactgcactt ctcacttctt ctaatacagc ctcattttct gaattcttaa gggttttaat
     5701 gatgagttca gccactttgc gactttcttc ttcaccaaat ccacgtgcag taatagctgc
     5761 tgctccgata cgaatcccac ttgtcttgaa tggtgacaag gtttcgtaag ggattgaatt
     5821 tttatttagg gtaatattga cttcatccag caagttttga gcaacttttc cgttttctac
     5881 aaccttagtc acatcaacaa ggaagagatg gttttcagtt ccacctgaaa taatacggaa
     5941 atcagggtct tgcaagaaga catctgccat agccttgctg ttcttaatta cattggcagc
     6001 atattccttg aaggctggat ccaaaacttc tttgaaggaa actgccttag ccgccacaac
     6061 atgctctaaa ggaccgccct gaatacctgg gaaaatagct gaattgattt ttttagcaag
     6121 ttcttcgtca ttggtcaaaa tcaaaccacc acgaggtcca cgaagggttt tgtgggtcgt
     6181 tgttgtagtg atatgagcgt atggcactgg gcttgggtga agaccagctg caaccaagcc
     6241 agcgatgtgg gccatatcaa ccataagctt cgcaccgaca gcatctgcga tttcacggaa
     6301 ttttgaaaag tcaataattt gagaataggc tgaagcacca gctacaatca gttttggttt
     6361 tacttcttgg gcttgtttca agatagcatc aaagtctaag agttccgttt caggatcaac
     6421 actataagaa acaaagttgt aggtttgacc agagaagcta acaggagccc catgagtcaa
     6481 atgaccacct gatgccaaat ccattcccat aaccgtatca cctggctcaa tcaaggacat
     6541 gtaagccgca cagttggcct gacttcctga gtgaggttgg acattggcaa atttagcacc
     6601 gaaaatttct tttgcgcgtt caatagcaag agtctctaca acgtctacta catcagttcc
     6661 accataataa cggcgtcctg ggtaaccctc ggcatattta tttgtcaaga tagacccttg
     6721 agctgccata acagccttgg aaactacgtt ttccgaagca attaactcga tattattttg
     6781 ttggcgttct tcttctttgg caatagcatt ccagagatca gcatcatatg ctttaaaatc
     6841 atctttgtca aaaatcatag gtcttctcct ttattgtgtg actagtccat tagtttgatt
     6901 ttacaataag aaaatcaaac taaaagatgc gaataaaccg tttctgcatt ttatcacaag
     6961 tatagccaac tttttcataa aatgcatgag cacccagacg atgattggca gaatttaagc
     7021 ggataaaccc ataaccacat cttttggctt cttgttccaa cccttgtagt aaacttttac
     7081 cgataccttg accttgcgct tgaggtgaaa ctgctaaagc taagatatta aatcctgctt
     7141 tggaatagag tgattcgtaa acttcagcgt ggacatatcc aagtaagaca tgattagctg
     7201 catcctcata gccaagtagg aaatgatggg aatcctgaga cagtctagct agttggctag
     7261 ccgtttcctc tggactaaaa gtataaccca aagcctcttg gttgatgtca catatcgctt
     7321 tcacatctgt ttcttgcaaa tctcttagca tttcaagcct cctcaaaaga aatctctggc
     7381 aaccgagcaa gaatatcttc tcgtttaatt gcgccttggc gtaagatttt caccttgtct
     7441 ccagacaaat ccacaatagt tgaatcctgt ccagttagaa aagcatcgtc ttccagaccc
     7501 agaacctctt ggtcaaaatc cttcagaatt tgttcaaagg ttacaccact tgcctgacct
     7561 gagatattgg cagacggccc aatcaaggga cctgtctctc gaattaaatc cagtgtgata
     7621 gggtgactgg gcatccgaaa tccaatagtt gcaaggtcag aatttaccca atagggaact
     7681 cggtcattgg cttcgagaat aatggtcaag ggacctggca aaaaggtctc tacaagtttt
     7741 tgtagataag ttggctgatt cttagaaaag tgcaagatat cctcgaaaga ggcgatattg
     7801 agattgagtg ccttatctct aggacgacgt ttgagttggt aaacatggtc aactgctttt
     7861 tcatctaagg ccttggcaaa aagaccataa acagtctctg taggtagaac gacagctcca
     7921 ccattttcca actcttgtct aatcctgtcc atcatcaacc acaaccatcc tattttgacc
     7981 aaattggtct ttgagtgttc gaactttttt ttcaggaaga tgttttctaa aaagttcggg
     8041 aacactttga ccttgcttgt atccaatttc aaggtaaatc ttaccaccat ccttgagata
     8101 gtcttttgca tcttccgcaa ttctacagta aatagctagg ccatcctcgt ctgcaaagag
     8161 agctagatga ggctccgaat gcaaaacatt caagcctacc tctgactcat cttcacgaga
     8221 gatatagggt ggattggata caattatatc atatttttca gaaatttctg caaaacaatc
     8281 agatttttta aaaaatatat taagattttg atcgctagca ttctctgatg caagctctaa
     8341 ggcctcttga gaaacatctg ctgctgtcac tgaccaatct ggtctatttt ttgctagagc
     8401 gagagcaata gctccactac ctgttccaat atccagaact gagagattcg tctcaagatt
     8461 ttcagttaga ataagctcca ctaactcctc tgtttctgga cgaggaatca aaacccgttc
     8521 atccaccttt aaatgcattc cataaaaatc tgcctgtcca atgatgtact gagctggctt
     8581 gtgagctgct agttgctggt aaatatcttc tacaaattgt ttttcttcct ctgttgtcac
     8641 ctcctgctgg agggcaaaaa taaagtctgt aaaagataga tttttcagac tacgatagac
     8701 aaaagagagg ctttccgctt cctctccttg tcttatcaac tcttcttcaa aatttgaaaa
     8761 taattgagct aatttcatta tttgtttaat tcttctagtt tttgtgtttg gtcataaagc
     8821 accaaggcat ccacaacttc gtccaattta ccagacaaaa tcgtatctag tttttggagg
     8881 gtcaagccga tacggtggtc tgtgacacgg ttttgaggga agttataagt tcggatccgt
     8941 tctgaacggt caccagtacc gattgtcgac ttacgctcag cgtcttgttc atcctgagca
     9001 atctgagcaa agtggtcagc gacgcgtgca cggataatct tcatagcctt ctcacggttc
     9061 ttctgctggg tacgttcttc ctgcatctca accttgatat tggttggcaa gtgaacgata
     9121 cgaacggcag tcgcaacctt attgacgttc tgtccaccag cacctgatgc gtggtaaata
     9181 tcaacacgaa gatcttttgg atcgatatcg tattctactt cttcgacttc aggcatgaca
     9241 agaactgtgg cagttgaggt atgaacacga ccttggcttt ctgtcacggg gacacgctgc
     9301 acacggtggg cacctgattc gtacttaagc ttagagtata cagactgacc tgaaaccata
     9361 gcaaccactt ctttaaaccc accgacacca ttcatagagg cttccatgac ttcaaagcgc
     9421 cagccttggg cttccgcata cttttggtac atagttagca aatctccagc gaaaagtgcc
     9481 gcttcgtctc caccagctgc tccacggatt tcaaggatga tattcttgtc atcgtttgga
     9541 tcctttggaa ggagcaaaat tttcagtttt tcttcatatt cttctttttc agccttggca
     9601 tctttgagtt cttgcttggc catttcttcc aagtccgcat ctccgcctga ttccttaatc
     9661 atctcttcgg catcgacgat attttgaagg acttgtttat actcacggta ggctattacg
     9721 gtatcacgat tggaagcttc ttcttttgaa agctccataa aacgcttggt gtctgaaacg
     9781 acatcagggt cactcagcaa ttctcctagt tcttcataac ggtcttctac agcttgtagt
     9841 tgatcataga tgttcatttt tcttcattct ccttattgat ttcaggggca aaataatgtt
     9901 tacggcaaac cgagatatag gtttcattac caccaatttg gatctgttct ccatcgtaaa
     9961 cgggcagtcc atcctgtgtt cgcaacacca tggtcgcctt tttcttgcaa tactgacaga
    10021 tggtcttgat ttcgtcaatc ttgtctgcta aaagcaagag atatttggaa ccttcgaaca
    10081 attcattgcg aaagtcattt ttcaagccaa aagccatgac gggtatgtct aactcgtcca
    10141 caacacgagc taggtcataa acatggtggc gtttgagaaa ctgggcttca tcgaccaaca
    10201 cacagtaagg tttttctggt aggcctcgga tatagccaaa gatatccgtt gtttcctcaa
    10261 tcgcaagggc agggcgtttc atgccaattc gactcgacac atagccaaca ccgtcacgcg
    10321 tatccagagc tgaagtcata atcacaacac cttttccttg ctcctcatag ttataggcca
    10381 ccttgagaat ctcgattgtc tttccagagt tcatggtccc ataacgataa tacaactgtg
    10441 ccatgtttct tgcttcacgt ccatttctaa atttttgcta cattctagta tatcataatt
    10501 ttcttaagct ttaaacggca aaatgtggta aaatggaaga aatcaaaact agtggaggaa
    10561 gctattatgc catttgtacg catcgattta tttgaaggac gcacgctcga gcaaaagaaa
    10621 gctcttgcta aggaagtaac ggaagcagtt gtccgcaaca ctggagcccc tcaatctgct
    10681 gtccatgtca tcatcaacga catgccagaa ggaacttact tcccacaagg ggaaatgcgt
    10741 actaaataag ctagcttaag cagaattgct taggcttttt caatctccaa gtagcattca
    10801 ttgaagaaat atcctaaatt tgttacaatt tgaaaagaaa cttggagaat ttccaagaaa
    10861 agagctatta attaaaggaa acattatgat tacacgtgaa tttgatacca tcgctgctat
    10921 ctctactcca ctaggtgaag gggctattgg tattgtccgc ctgagcggaa cagacagttt
    10981 tgctattgcg caaaagattt ttaaaggaaa agacttgaac aaggttgcca gccacactct
    11041 caactacggt cacattattg atcctctgac tggtaaagtc atggacgagg ttatggttgg
    11101 ggctatgaag tctccaaaga ccttcactcg tgaggatatt atcgagatta acacccacgg
    11161 tggtattgcg gtgaccaatg agattctcca gctagctatc cgtgaaggag ctcggttggc
    11221 agaacctggt gaatttacca aacgtgcctt tttaaacggt cgcgtagatt tgacacaggc
    11281 cgaggcggtg atggatatca tccgcgccaa gacagacaag gccatgaaca tcgcggtcaa
    11341 acaacttgat ggttcccttt ctgatcttat caataatatc cgtcaagaaa tccttaacac
    11401 acttgcccaa gttgaggtca atatcgacta tcctgagtat gacgatgttg aggaagccac
    11461 tactgctgtt gtccgagaga agacaatgga gtttgagcaa ttactaacca agctccttag
    11521 gacagcacgt cgtggtaaaa tccttcgtga aggaatttca acggctatca ttggacgtcc
    11581 caacgttggg aaatcaagcc ttctcaacaa cctcttgcgt gaggacaagg ctatcgtaac
    11641 cgatatcgct gggacaacac gagatgtcat cgaagagtac gtcaacatca atggtgtacc
    11701 tctcaaattg attgatacag ctggtattcg tgaaacggat gatatagtgg aacaaatcgg
    11761 tgttgagcgt tcgaaaaaag ccctcaagga agccaacttg gttctactag tgctaaatgc
    11821 cagtgaacca ctgactgcgc aagacagaca acttcttgaa attagccaag ataccaatcg
    11881 cattattcta cttaataaaa ccgacctgcc agaaacgatt gaaacttcga aactacctga
    11941 agacgttatc cgtatttcag tccttaaaaa ccaaaacatc gacaagattg aagagcgaat
    12001 caacaacctc ttctttgaaa atgctggctt gattgagcaa gatgctactt acttgtcaaa
    12061 cgcccgtcac atttccctga ttgaaaaagc agttgaaagc ctacaagccg ttaatcaagg
    12121 tcttgagctg gggatgccag ttgatttgct tcaagttgac ttgactcgta cttgggaaat
    12181 cctcggagaa atcactgggg atgctgctcc agatgaactc atcacccaac tctttagcca
    12241 attctgttta ggaaaataag aaaaatccat gatccttcat tcggtcatgg attttattgt
    12301 ctttattagt aatctggtct taagacccct gttacagttg ccttagttgc ttcgtagtcg
    12361 ccatctacga caaccttgat aatgcgtttg gcatcttctt ctggtgctgg aacaagaggt
    12421 agacgagtgg gtccagcttc aaatcccata tagttaagaa ttgccttaac tggagcagga
    12481 cttggataag agaagagagc attaacctta ggaatgaatt tacgctgaat tgctgcggct
    12541 ttcttcatat cgctttctgc aatggcagta aacatctcgt gcatttcatc cccatttgta
    12601 tgagaggcaa cagaaataac cccatccgcc ccaaggttca tggcatggaa agcatctcca
    12661 tcctcacctg tataaatcaa gaactcttca ggcttgtgct caatcaagta agccatatta
    12721 gccaagctag tacattcttt gacaccgata atatttggat ggtcagccaa gcgaagcatg
    12781 gtttctggag tcaattcgac aactacacgc cctggaatgt tatagataat aattggtagg
    12841 tcagaagcat ctgcaatagt cttaaagtgc tgatacatcc cttcttgaga aggtttgttg
    12901 tagtaaggaa caatagcaag cccagctgcg aaaccaccaa attccgctac ttctttgaca
    12961 aactcaatag agtcacgcgt atcattagta cctacacccg caatcaaagg aacgcgtcca
    13021 ttgacaacct tttgtacagc cgcaaacaac tccaactcct catcgtgggt caaagttgga
    13081 ctctcagcag tcgttcctgc gagaagaatt ccatccgtat gatgggccaa taaatgctca
    13141 atcaaggctg gaatagcatc aaagttaata gaaccatcct catgaaaggg ggtaataaag
    13201 gctgtaatga ttttacattc ttttaaatct tgataagaca tgaaaacacc tctctatttc
    13261 aaagaaggag ttcatctgaa ctcctaaacg atatgactat tttaattcaa atttcaattc
    13321 ggctgttgga cgaaccaatc cacgttcatg aagagtttca gcaatctgaa ctgagttcca
    13381 agcagcacct ttgagaaggt tatctgaaac aacccacatg tgaattcctt tttctgcatc
    13441 caagtcttta cggatacgac caacaaaggt atcacgcgaa ccaactgcat tgatagcttg
    13501 aggatagatt tgatgagcta catcatcttc aagaacagca cctgggaagg ctgcgatagc
    13561 tgcttttact tcttcgattg gagccacttc ttttgtttcg atataaacag actcagagtg
    13621 agctgacaag actggaatac gcacacatgt tgcagatact gcaatgctat catcttccat
    13681 aattttctta gtttccttgg tcatcttcat ctcttcgtac gtgtaatcat tatcagtgaa
    13741 aacatcaatt tgtggaagag cgttaaaggc gataggataa tgtttcttgt caccacctga
    13801 aggcaaaatt tccgcatgca aatcacatgg tttcacacca tcattcaaga cttcacgaag
    13861 ttcacgttgt gtctcaagaa ttgctcccat accagcacct gaaacggctt gataagttga
    13921 aacaatgata cggtccaagc cccatttttg gcgaaccggc tcaagagcca ccatcatttg
    13981 gattgttgaa caattagggc aggcaatgat tccgttgtgg gcatcaagtg catgagcatt
    14041 gacctctgga acaaccaaag gaacatctgg attttgacgg aaataagatg tattatccac
    14101 tactaccacg ccagctttta ctgcgtatgg tgcatactta gctgatgtag aactacctgc
    14161 tgaaaagaga gcaatatcaa ctccttcaaa agctgtttca gtcgtttctt caattgtaat
    14221 atcttgatct ttaaatttca atgacttacc tgctgaacgt gcagaagcaa ggtaacggat
    14281 tttatcgatt ggaagtgttg attcttccaa catttttatc atctgagcac cgacagcacc
    14341 tgtcgcgccg actacagcaa ctgtatatcc cataaataac ctctttcgga attttctaaa
    14401 aatttctata atagaagtat tatactactt tttccatgaa ttgcaaagct ttttcatcat
    14461 tttttggaaa ataaaaatcc cagtcgctag actagggact aagaggcatc ttcatgtgat
    14521 gagcaggttc acacaactca tcaaggtccg ctcctgcgtt atgacctcct tatgctcaat
    14581 agtagtccga agactaccta tcgactcatc gcatattcta tattactaga gatacttgat
    14641 tttgtcaata agaaattttt tcgatcgagt ataacaaaaa actctcgtat tcaagagttt
    14701 ccctatacat atcatgctaa ttgccgggat cgaaccggcg acctcatcct taccatggat
    14761 gcgctctgcc aactgagcta aatcagctta cttaagaagt atactatatt cagaaaaact
    14821 tgtcaagttt ccttagaaat tttccatagg aaaaagccag gtaagaacta cctagcttat
    14881 cttcttctat ttacttaaat caattcgacg accaatttta ccaataataa tcgccccaat
    14941 aatcaatgaa gcaaattcac cgattcctgt tgtaaaccaa gtaaggaaga atggtaattc
    15001 tgctacaata tttaactctg cagcgatagt aaacatggaa attgaaaaga ggattgaaaa
    15061 gaagaaatga tcttttcgaa tcaatccgtt aaagaggtaa tccttgctat attttgcaaa
    15121 aagccaaaca cctaaactga ggaatactaa ggttgaacca ccaccaacaa agacatctag
    15181 cagtccgaag ctaaagaaat tagcaatcat acaaccgatg gtaactccga tgatgtactt
    15241 aggattgtaa aaagccataa agttcatcat ttctgaaata cgaaactgat aagcaccata
    15301 gctgatggca tttaggggcg gtgtgacagt caaaacgacg tagatagcag tgacgattgc
    15361 aatatctgca atatcacgaa tagttaattt tttcatcttt tctcctttgg cggttgcccg
    15421 cgtgtaatat gcttggtgaa agaagctaag caccaagggt tgctttattc aaccctacta
    15481 gtatagcaca atagcctgct ttatgctata cttaacacat gaaaattcct attcaaaaat
    15541 tttttaacaa tgaaatctta gcctatctct tttttggcct tgcgacaact ctagtttcga
    15601 ttctctcacg actagtcatt tatcaactaa gtcatcagga actcctggct actgccctag
    15661 caaatattat cggtatccta tttgccttta tcacaaatga tacgattgta tttaagcaaa
    15721 aaaagaaaaa tagacttatc cgtttagtga aattttctct tgctcgcctt tctacttttc
    15781 tgttagactt gtttttaact tttctctttg taacccaatt tcctcacatt atagggcaat
    15841 tcgtaactga aaacattgat aagataaatg ctattgaaac aataattgca caaatattca
    15901 ttattatcat caattatata ttgagcaagg cctatatttt tagaaaataa tgctccattt
    15961 atgagccttt taattgcctt ctataatgat ttaggataca atgaaacacg aaacattgaa
    16021 aggaaaacag tatgttaaaa aatctaaaat cgttcttgct tcgaggaaat gttattgacc
    16081 ttgctgtcgg tgttgtaatt gcctctgctt ttggtgctat cgttacttca cttgtaaacg
    16141 acattatcac tcctcttatt ttaaatcccg ctttgaaagc tgctaaagtt gaacgtatcg
    16201 ctcaactttc ttggcatgga gtcggctatg gtaacttctt aagtgctatt atcaatttta
    16261 tctttgtggg taccgccctc ttctttatta tcaagggcat tgaaaaagca cagaaactga
    16321 ctggcataaa gaaagaaaaa actgccgaaa aaaaaccaac cgaattggaa gtccttcaag
    16381 aaataaaagc tctccttgag aaaaaataat cgattaaaag gatctagcgc aaagctaaat
    16441 ccttttttct ttactctttg ggtaacttgc ctgctttttc aagcattttt ttgatcagat
    16501 aaggcatctt aacattctcc cgcccttttt tctcaatcag ttttttcaca aattctggca
    16561 tttgtaaatc ttcagattca gctaaaatgt ttttcgtctc gtctgaagga actaactcat
    16621 caaaggtctc ttcttctggg agaaattcct taaactcttg attttcattg actctaactg
    16681 tattgaccaa agcatcaatc aaacgagaat ctgtatttgg cattggtgga cgatggtagt
    16741 ttaccccaaa ttcctggcat aagtcataac attctacatc attgtcaaac aagacctcaa
    16801 tatgctcact gatgaaacta ataggtacaa aaatataatg gtctggatgt tctgtctgtt
    16861 ctctgagata ctccaaaaca tctggtttaa tccatggaat accaatatca ctctcactct
    16921 gccaagtgtt ggtatattgt tctgaactca agcccagttt ttcagcgact agcttgctat
    16981 tttcgaaaat ttggtctata taaggatcac caaagtccaa ggcaaaaatg ggcacactat
    17041 gggctgaaaa gatgacttta aagctgtctt gctttacttc ttcttttaaa atcttagcaa
    17101 tttcatctgc ccaatagttt aagagcactt cttcctgata ccagtcctta atgaccaaaa
    17161 attgaatttg tttgctttct aaaaattttt catagcccat gacagagtag aaagaataat
    17221 ggggctccaa aatcaagcag atacattctt caacaccatc agtttccatc tgcttgatca
    17281 cgtctggaat aaagggacga gaaaatttgt tggcaaagta gataccgtac tcctccccta
    17341 aacgttcttt gactaaattg acctcctcac gtgtaatccg ctgcaaaggc gtcccaccga
    17401 tacgaatata gttgtcatag agagtctgga tttcgtggtc ttggggtcta actccacgac
    17461 gaatgtttgt aaagaattct gctactcctt caaaactaat ctcctctgga gatccaaagg
    17521 tcatcattaa tatggctttt ttcatgttat catccttatg atattatttt ctctatccat
    17581 tctatcatga aagaaaaaaa tagtaaacgt ttcctacgtc tactatttct tttgctcttt
    17641 tgttttttta tctcgtctta taattaggat gataaggaca agaagtaaga ggacgataat
    17701 aaggagtaga aaggctagaa tgtaaatcca atgactggga attccgtgat cgaaataccg
    17761 tgttcctact gcttccccac tttcatcctg tgctactttc accggtgcat ccgttatttt
    17821 agctaaaagg atatgtcgtc cattagttat gttaaggaaa catctcgctt gtacttggct
    17881 ttacttagca gatattgata gtaagcctgg tgttcctcat ccttggaact caaggttcga
    17941 taaatctccg tgtcataggc atccacttct aaaatcccaa aaatttcaag accacgttct
    18001 cgaccattgt agtagataga accgtagcga tgctggtcga agaattcctt atcagcaaat
    18061 ttcttaatct caccgaacat agcccctgaa gccatagagt gtccgtagat gatcgtatta
    18121 aaatcagtaa agttgggatt aaagcgatag tcgagaaaaa ttgatcccat tatcgtataa
    18181 tcgccaaagg cattgcggtc aaggtatttg acattatcct gactttgtag aagcggatag
    18241 tctaccttgg tcccatcaag gctaatccaa ccgcaaacat ctttgttcaa gtgctgtagg
    18301 gtttcaaaac ctgtgctcga agcatccttt tcaataggat tatagtgatc atagtcactt
    18361 gcttctacta ctctctgcca actaaaaaga gctacaaaaa cacagaagaa taccagaaat
    18421 acgtttttaa atcgcgctct tttttgaacc gtcatcagca ctcctttcta tgcgagattt
    18481 cttaaaagga aaggaggcgg gacaaaaccg cctcttttct attcaccctt agtcagtaaa
    18541 agggtctgct tcttgcattt tttttactct ctcttctacg acgtaggatc aagtagagga
    18601 caaggagtaa gaaaatcaag agcaagaaca atacaaacaa gataaggtac caaatccata
    18661 gtgggattga tgagaggaaa cgtccaagag acgagtcatc aaagacactg tatggaaatg
    18721 gttttgattt ttttgtatgg aaagtatttt tagggacggt gtctgtaatc tttgcgacta
    18781 cgatatgacg accattggtc acatcgagaa aacaggtact gagtaggatg atacgatcac
    18841 tcggtgagag tgagatatcc cgcttgtgca tggcgactga gagcaggtgg tctagatagg
    18901 cctgctggcg gtcctcaccc tgtattcctg gatcatagat gttaaagtca taggcatcaa
    18961 cctccaacat ctcaaagatc tcgagccctt tttccacacc attgtagtat atactaccgt
    19021 aacgatgctg gtcaaaaaat tcctgatcag caaacttagc cacatcacca aacatgaccc
    19081 cattttctac gtggtgcccg tagataatgg tattaaagtc ttcgaactta ggattatttc
    19141 gtgcatcgag aaagatagcg cctgtcgctg caaactcacc tttggagtcc ttgttgagat
    19201 acttttcatt gtccttggct tggactaagg gatagtcgat attggtgcca tagacattga
    19261 tccaaccgag gacttcggga ttgagttctt ggagtttgct gaagcctgaa aaactggcca
    19321 gctcatcctg ttgcgtcgtg acaggtcgat aagcttcgta ctcacttgac gaagctaagg
    19381 agtagacttg gttggaatcc caaagggcgt agaaaccaaa gagaaagatc aaagctgcaa
    19441 aaatgagcag caagtgattg atcaccccat ccacagcaag aacgagacgc ctagacagac
    19501 tcttttttgg tttctcacgc ttggttttca tcatctgccc ccagccgttt aggcttctct
    19561 acgtttttta gacaagacaa caaatccagc caaaccaaga ccaatcatca aaatgaatgg
    19621 aaggttatca atcaagagac cagtaggagt aacgtcgtcg attttgttag taactttgtt
    19681 gtcgtttttc ttttcaccta tgaggatatc tgttaatgta aaatctacat ttttttgtcc
    19741 ttgagatgtt tgttctgcac catttgtagt ataaattgat gaatctgtat aacctgctac
    19801 tccagtttca gttactgtat atttagaacc tgctggtagt tgttcaactg taaatgtttc
    19861 accatcttta agagtaaaat tgtactcaac tgtatctgct ggggtcacta ctgccaacac
    19921 ctctgactta gatccatgtt ttactataat attcgtaaca ggggtttctg ctgttttatt
    19981 tgtacttgga agttgaatct tgatatggaa ttggaaatct cttgttttat cccctgaagc
    20041 tccatcaact tttttagtaa ttgttaatgc tttcgcattg gggtctactt gactcggatc
    20101 atcaggatta ggctccttac ttgcgtcttt ggtatatgtg ttatcaaata aattataaac
    20161 gccttgttca cttggttcta ctttaggggc gttagattta ttattttcct taaagtatac
    20221 tgatgaaata tagacaccac catttacttt attcttaaca ataacgtgca tttcatatgt
    20281 acgtttgtcg tatctcatgg catcaattgt tttaccattt ttggtaatcg cctgccatcc
    20341 agtttttgct tccgctacat cataaacgta ttcaccagca tggccataag caactcccga
    20401 aaaaatgtct tctgttgctt tttgaatctt atcagcagtt gggtgatcat ttttattgta
    20461 ttctacattt ctatctggaa ttgcaactcc ggtttgatat ggcgcaccgt tagaagattg
    20521 tcctgttttt tctgtaaact taaatgtaaa tgtcgctgtt ggtgttgtta ttccctctgc
    20581 aatattcaac gttttattaa tcttcacaac agcattattt tcaccatttg ctttggttat
    20641 tgctgaatta tcatccgcaa aagtcgggac acctctgaat acggacaaag cggttatagc
    20701 tgcaatgctt gcagtgaata gctttttaaa gaatgttttt ttcatgatta tttcttttgt
    20761 ttttttattt tcttttttca ttatgatcat caaaagcctc gtcttctaat gactgttaca
    20821 actttgcctt gagtatcctg tatagggatg gttccaaaag cacgactgtc tacagccgtt
    20881 gttcgattgt ccccgaggac aaaaagttgt cctgctggga ctttcatcgg gaaggttgcc
    20941 ccttccttat agagcagcgt ttctttgtag atatcttgct cttgttgagg agaaccgttg
    21001 atgatgagac cattttcgtt tatatcgatt gtacttcctt cggttgctat gacacgcgcc
    21061 actctttcct tacctttata actatagact agcaaatcac caatcgaata gcgtttatcc
    21121 aacctataat agacgaccaa gtcgccatct tttaaggcgg gcttcatgcc atcgtcattg
    21181 tatcttaata atccaaagac gaaaaagtaa aggatggcta gtacaaatcc aaccatcaga
    21241 agtttactga ctaagtaaag gatatctccc cagacactcg gcggagactt atcccgattg
    21301 acttgtgtta ctggtttctt atgtttcttt ttaagcagca tcacttcctc ctattcacca
    21361 caaggtgacc aagtaagatg ataaagaaga tccctcctag aggaagaacg actccaagca
    21421 cgagagtgct actcaagaag tcaattcccg tttcaggtac gctgttttta ttattcgtga
    21481 ctgtcgcaga cttatttgca tcaagagttc cttctttttt atcataggac acctgatacc
    21541 cacgagacga acttgcttct tcttcgatac tataacgagc atttagagga aggtcgagaa
    21601 tcttgatggt tttatctttc tttagatcaa ctgtcgcctt accattggtg aattgtagcg
    21661 ttgttttttg attatttact attgcactat acgatccatt caatggttta ttttgcgcat
    21721 cctttacgtt aatggtaatc ttaaaggatt gcagaagatt agcaaaagca cctgtaactt
    21781 tttttgtgac tgaaagtgtc ggaagtctgt ggttggtgac gattgtttga acatcctctg
    21841 acaaagtccc ctcttgtttt tcataggtga catgatatcc cgttctagag ttctctactt
    21901 cttccacctt atagtgggta tcacgaggca gataactgag ggcagtttcc tgactatttt
    21961 tgagagaaat ctgtgctttc ccgttagtca ctgttgtatt cccaaacttg ccgttaatcg
    22021 gcttgccatc cttatctgaa aaggtaacat taattgtgaa agatttatta ggtgattgtt
    22081 caccttctag gattttcttt acggtgagac ttggaagctg tcggtaaata actttgaagg
    22141 atggtttctc tttcgtacct gtcttttgaa cttcgactgt gtatcccgga gcgtctggtg
    22201 ctttcagttc gtagtcatat cccttagcta cctttgcaaa actagtattt tcagtaaaac
    22261 tattcgtttg aactggacta gtgacctttc gataggaatc ctttatactt ccatctttct
    22321 tttggtttaa gtttaattcg acactaggtg gacgatttgc tgatggattt gattttccat
    22381 ccacaccttt ccactcaatc tctacaggaa ccgtcaatgg atctgacggc ttgaaagtgg
    22441 gcttctcaga atagtctttt attttggtac gtgaggtccc aataccatag gaataggcaa
    22501 cttttgcccc cttgttggag tagagtttag tagcatcggc ttcttcggca tcatcactag
    22561 taagtgtctt atcacccgca atcgcatcaa atgcctcttg gctagaggtg acagtaaact
    22621 tgagaacgta cttggcgtca tcctccaaag tgtaactcgg attaaacttg gctgttactt
    22681 ccactaatcc ctgttcgttc ttcttagttt caatcgtaac ttgattgtca ttcagttctt
    22741 gctcgttacc atccttgatt ttgacgacac gaaggtgtga agcatcacca ggtaacagct
    22801 gcacgtactt ggacaagaca tctgatatag ttacatggtg tacgcctaga ggtagaattt
    22861 tatctgtgat ttctttgaat ttttgttgca agtcattttc attattggca gaaaatattt
    22921 cgttggggat tccagaatta taaccactga tatatccttt aagagatgtg atactatcgt
    22981 ttgaacctat atatctgaat ttgattgaat aaaagccatc tatttttgga gctattaaac
    23041 tagatatatt cagcattcct ctctctaaac cagaggtaaa ccaatatgga gtatcaatca
    23101 tacgtcccac atttggatta gaataatagt tatatattgt tctcccacta ttagactcgt
    23161 aatacatatt agcttcgcca tctgacagca gaataactac tttttttgcc ccagcccttg
    23221 cactttgcaa caatctttga gcttcagtta acccagcatt tatatttgtc ccagtgccaa
    23281 tgcccgcgtc cataccataa gaacgactag agtcagcaat tgttaacgaa ctaacaatat
    23341 tttttgagct attagaatcc gtgctccaag atactaaagt tttagcatca tcatatgaag
    23401 agatatttga ataccaacca gctcaatttg gccaagttcc atattgataa ctcagataat
    23461 attcattata ataacggtca ttatactgat tatcaatctt tccggcaaaa ccaactatag
    23521 acagacggtt ttgggaatta gaaagaattg tatcaatgag cccctgacga ccattcacac
    23581 ctttcagtgt atgttttagt gcttcaatcc tactaatgta tcgtctacta gagtaagacc
    23641 acacatctcg ctcttccata ctccctgaaa gatcggcaac caagactaca tccaagggtt
    23701 ctgactgggt ctccgttccc agtttagatg tgatatccag tgacaactca tactggtctt
    23761 tttaccctga tatcggagta atagttttgt gcagagttgt ctgaggttca gttattgaat
    23821 ctgcacgtaa agaattaatt ggtgcacaca aaaaaagaat agcgagaaag ataactaaac
    23881 tactggtaaa atattgaatc ttcttaaaat catattgaac gttcatgcta tctcccttgc
    23941 tagaatatgt tttaatctga tactatataa atctgagact tccataagat aattgtataa
    24001 taatattaac ttcgtgtcaa ttaatgtgtt tgaaaacatg ttattataaa ttacaaaaaa
    24061 gtatgattct tctttgtttt tcaacttttt ctgtggtaaa atggaaaaaa tagtaaaaaa
    24121 tttcataaaa taaagatgca tatatttatt ctaatcgctc gaaaatttct tttgaaagtc
    24181 ttctgaattt gaattattat attttttcaa aattcataaa atttctcaaa tcatattttt
    24241 ccgaagtttt gtattctatt ttatgataaa aaaggagttt ttttatgact tatccaaatc
    24301 tcttggaccg cttcttaacc tacgttaagg tcaacacgcg ctctgatgaa cactctacta
    24361 ctactccaag tacacagagt caggttgact tcgcaacaaa tgtcctaatt cctgaaatga
    24421 aacgtgttgg actgcaaaat gtttactatc taccgaatgg ttttgctatt ggaaccttgc
    24481 cagccaacga tccgtcttta acacgtaaga ttggttttat atcgcacatg gatactgctg
    24541 attttaatgc tgaaggagtc aatccacagg taattgaaaa ctacgatggt ggtgtgattg
    24601 aactagggaa ttctggtttc aaactcgatc cagccgactt caagagtctt gaaaaatatc
    24661 caagacaaac actcatcaca acagatggaa caaccttgct aggtgctgat gacaagtcag
    24721 gaattgctga aattatgaca gccattgaat atctaactgc tcatcctgaa attaagcact
    24781 gtgagattcg tgttggtttt ggtccagatg aagaaatcgg tgttggtgcc aataaatttg
    24841 atgcagaaga ttttgatgtg gattttgcct acactgttga tggtggtcca ctaggtgaac
    24901 ttcagtacga gactttctca gccgctggtg ctgaattgca tttccaaggc cgtaatgtcc
    24961 accctggtac tgccaaaggg cagatggtca atgcccttca gctagcaatt gattttcata
    25021 atcaacttcc agaaaatgac cgacctgagt taactgaagg ttaccaaggt ttttaccatc
    25081 taatggatgt gacaggtagt gttgaggagg tgcgtgcaag ctacatcatt cgtgattttg
    25141 aaaaagatgc ctttgaagcg cgtaaagcat ccatgcaatc tatcgctgat aagatgaatg
    25201 aagaacttgg gagcgaccgt gtcactctca acttgacaga ccagtactac aatatgaaag
    25261 aagtcattga aaaagatatg actccaatta ccattgctaa agccgttatg gaagatctag
    25321 gtatcacgcc tattatcgaa ccaatccggg gtggaacaga cggctctaag atttccttta
    25381 tgggaatccc aactccgaat atctttgcag gtggcgaaaa tatgcacgga cgttttgaat
    25441 acgttagcct tcagactatg gaacgtgcag ttgataccat cattggcatc gtagcttata
    25501 aaggctaaaa agacgaggtg gctcagctac ttcgcctttc tttttattct actggttttt
    25561 cttgatttcc agtagttgta gaagattctg ttgtttcatt ttctgaagtt gattcagcag
    25621 gtttagaatc tcttgtattg cttggtttgt tttcgtcgct agcagtttca atgttagatt
    25681 ctgcagttgc gtttggttgg ttctcagcac tggtgttatc actatttgct tcagcatttc
    25741 ttgctggact tgtttcttca cttgcgctaa cttttgactg gatttgatga ttcaaaacta
    25801 gaatagcttt tgtcgattca agtaaagctg ttttgtcttt actattagca gaaagttgat
    25861 ctaataatgc atccacctta tcaaagtccg catcagatcc attattactt tctaaataag
    25921 agtgaagcga catgagaata tcgtagagtt tttgatagag tacaagtgtc tgagaatctt
    25981 gctcagcatt ttccttttct tgttgaaggg cgctagcgat acgagtcaaa acatctttta
    26041 cctgactgtt tacttcatcc aagtctgcat cagccttgtt tgtggcagct tttagatttt
    26101 ctacttcttc tgccaaggat tgtctgattc cttcttcatg gatttgttcc aagagttgat
    26161 ttgccttgtt caaaagactt tctacttctt ccttgctatc tgtcgcagat tattggttgc
    26221 tatctaccat gtactcctaa aacaggagag ttataatcca agattacaag gccttacaga
    26281 aataagaaat ccagataaga caatgttcgt ccaagacgct attcgcttcg cacagcagca
    26341 cggattcaat atgctttaat tttaaagttt aggtgtcaag acctcttttt agtgtgccca
    26401 aaatttagag aagtaatcaa tcaactaact tttatttttt tcaaactttc agtaaactga
    26461 cctaaagcta actcaatctg tctttgtaga tgcttctgct atcagctaga agttgatcta
    26521 cttttgccaa gactgccttc tcatcaaaag ttccaggttg atagttggat tgcagggatg
    26581 gaatcttgtt tttcaaagcc gcttcatatc ccttagtttg aaccttgatg tagtgattgt
    26641 ggtcgccatg aggaatcaca aaaccttctg aatcttcact tataattcga ttggcatcaa
    26701 aaaccataac catcttcttc ctcatggtgg acatgtagtg acggattact taatacagaa
    26761 ctagaagaac ttcctacctc ttccgtgtta gagtgtgatg ggggattgtt aagagatgac
    26821 ttaggaatat agtgatagtg ttccccatgt cttactatat aagcatcacc tatatctctg
    26881 acaatatcat tagggttaaa gacatatgtg gctgctaatt cacctgccga caagtcactc
    26941 tcaggaatga aatgatagtg accaccatgt ggtactatta gtagattgaa atagaatatg
    27001 agcaaattga taaggggatt ttaaagtaat ttctaacaat gatttagaaa ctatgatgtg
    27061 ctattctaaa ttcaactcac tatatataac catcatcggt agtataacgt ccctgtaatt
    27121 ttgctacaga tacttctgca ctagctcctt tatcgtcttt accatgttct tgtttttggc
    27181 gattgatttc atcttttgtt cgtacatttt ctgcatgagc ttgatcttta aggtaaacat
    27241 aatactttcc atctacctta ataatatatc ctcccttaac ctaactgaca atatcttgat
    27301 ctttcggctg atagttgggg gctttcatta atggctcttc actaaagagc gcatcaaaag
    27361 gaactttacc attatagtag tgataatgat cgccatgaga agttacataa ccttgatctg
    27421 taatcttaat aacaatttgt tttgcttgaa ttccttcttt ttgactaacc tagtctggag
    27481 tcaaattttc agtcttctta gtgtctttat tactgtttac atatgaaaca cgatttttat
    27541 ctgtattggc ctgttagcta tgttggttca gagcataaac acacagactt aaggaaagga
    27601 taacaacaga tccagctgct atatatttct ttttaaattt cataattacc tcatttctat
    27661 aattatttat atgatgtctt cattattaaa tgattataga aattaattaa ccaattaatt
    27721 aactagtaaa tattccacct ctttttaagt tgtatgtcaa gaaattttat atattaataa
    27781 taaaatgaaa ttctcccaaa gtcagagttt tatttctaac ttttgagaga acttcatttt
    27841 tgattcagac tttttctact gctattcctt acgctatgag atcagataaa ttctttttta
    27901 tcacttctcc acttggcaat cttaattcaa tcgttccatc catattgaat ataacactat
    27961 ctaagcctaa tccgtaacta gctgtaaatt tttctaattt ttcttgtaca ggatctactg
    28021 ctggagcttc ctctaatgct ggatctaaca tagggtcact ccccacattc ccttctggat
    28081 tcaacattcc attatccgtt gagttttctg gttttacagg tttttcgttt ggtgcctctg
    28141 gtaaagaatc tgctggttta ttttctgttg gttggttctc aactgttcca gtagatactt
    28201 ttccattttc agatggttta ttttcaccat ttccttgagg tgcttctcct gtaaaatctg
    28261 ccatattctt tttaatgact tctcccgatg gtaaatataa ttcaattgtt ccgtccatat
    28321 taaacaagac attttctagc ttcatcccat aactttcagc aaattttgct actttttctt
    28381 gtacaggatc cactgtagga acttcttcta acgttgaatt actagtacta ttcccagttt
    28441 cagaaagttt ttctttttct accttctcac tagtctttgg ttcttctacc ttttcatcaa
    28501 gttttgagtt ttcttgtgct ttattccttt taaattgtgg tagaatactt ggtttatcag
    28561 tttgattttc tttttccaag ataggtactt ccacaatata agtcgattga ttgtccaaat
    28621 aagcatttgc catgaaggtt acaggaattt tatttccggc cgttctggtt gttccttggt
    28681 ttaatttcgg aatcggtaat ttgatttcac caactttata gttattttct aaataagcat
    28741 ttccatgaaa ttcatcaaac actctgacta aagcatcagt tcctttaggc actgcaaatt
    28801 gagggttcac tcttaaataa gtatcccctg catggaaagg atagaaaatc gtttgactgg
    28861 ccattttgta agctaaagag gttggaactg taaatgtacc atcataactt acttctggat
    28921 aatcttttga agcgatagta tacttaaatg tttgtcctgg taaataaggt tgatctaatt
    28981 caaagtttgc aatattccct actccttctc caaatacttt accagatact ttctccaata
    29041 cttttccatc tggtgttatt aattttacta gcatattgat acctaatttt ttctccaatt
    29101 caggcggaaa actaaaagaa acgcgttttt gaccattggc tagagtaaag ttttgattat
    29161 taaacgtact attttttaac aaattaacaa cattcgttaa ttcttctcca gtataaactt
    29221 tattcccttc ttttttagca actccttctt cgggtttaaa cagttcatag ttactgtgag
    29281 aatgaccaat tccaaccggt ttatgttcat caatcggatc tgcatgatgg tgatctccat
    29341 gcggataaat aatcgcattt ttttctttat tcacgacaat actttcacgt ttgacaccat
    29401 attgtttcat aatgccagca attttttctt cgattttttt atctaaatct ttcatttctt
    29461 tggcattact tggataatcc tgttcatgag atgacaaaga atctaatcca ttatgactag
    29521 ttttaacttc ctctaaatgt ttttgcgcag ccttaatttg ctcttctgtc aagtccttct
    29581 tgaagaaata atgattgtgg tctccgtgac tcatgacaaa acctgattca tcttcagcga
    29641 taatacgatt agcatcaaat ccgtatccat cttcttcatg tttctcatgt gaagttcctg
    29701 gattgattgg aagagatgga gaaggtgttg ctagactatt gtttggaaga gtcggttgcc
    29761 caatttgatt tgattttgga atgtaatgga aatgatcacc atgtcttaca atataagctg
    29821 tagccgtttc ttcaacgata tcttttggat taaaaatata accatcagat gctgaagaga
    29881 gctccttact tgtcgttaaa gaagaaggat tgcttgaaag actgcctaga ctagacacta
    29941 cttcattagg ttttgcattt gtagaaactg tagaaccagt tccactgata ggcaccattc
    30001 tggcaatctt ttcttctaag gcagaaagct tgctgtaagg aataaagtgg taatggtcgc
    30061 catgcggaat cgcaactcca tttggtgtac gactgataat cttagcaggg tcaaagacca
    30121 ggccatctga ttcactgtaa cgttgggcgc taggtgaatc atagagttcc ttcaaaagac
    30181 tctggagatt ttcagattta tttgctggct tgctagttga tccttttgct acagattgcg
    30241 tgttattgtc actagctgtt gaagaatagc ttaactgact cggttgcata ttttttccag
    30301 ccagatgtgc tttagctgct gctaattcac tagcagataa atcgcttttg ggaatgtagt
    30361 gatagtgacc tccatgagga acgatataag cattacccgt atcttcgata atatcagctg
    30421 gattaaagac ataaccatca tttgtcgtat atcgtccctg agaccttgct acagcaacat
    30481 tagagttaac cttctcatta tctttgacat gttcttgttt ttgacgattg atttcatctt
    30541 tagttcgaac attatcagca tgagctgcat ctttcaggta gacataatat tttccatcga
    30601 ccttgatgat ataaccaccc ttgacttcat tgacaatatc agcgtcttta agttgatagt
    30661 ttggatcctt catcaagagt tcttcactaa agagggcatc ataaggaact ttcccattat
    30721 agtaatgata gtggtcaccg tgtgacgtta catagccctg atctgtaatt ttgattacaa
    30781 tttgctcggc ctgaattcct tctttctggc taatctggtc tggtgtcaag ttttcacttt
    30841 tctgacttga ctggctgcca tctacataag agacacgatt attgtcctta ttttcctgcg
    30901 aacgatgctg gtttagtgca taggcacata gactcaagga tacgataaca gctgatccag
    30961 ctgctatata ttttttactg aatttcataa atccctcatt tca
//
LOCUS       DAVBCW010000014        29514 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVBCW010000014 DAVBCW010000000
VERSION     DAVBCW010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29514
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.014"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            184..414
                     /gene="blpU"
                     /locus_tag="VQ514_000542"
     CDS             184..414
                     /gene="blpU"
                     /locus_tag="VQ514_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HET2499344.1"
                     /translation="MNTKKMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAVRGLQ
                     LGIKTRTWQGAATGAAGGAILGGVAYAATCWW"
     gene            1129..3282
                     /locus_tag="VQ514_000543"
     CDS             1129..3282
                     /locus_tag="VQ514_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HET2499345.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPYKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTGINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLMALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            3295..4644
                     /locus_tag="VQ514_000544"
     CDS             3295..4644
                     /locus_tag="VQ514_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HET2499346.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAMETGASLASQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            4814..5521
                     /locus_tag="VQ514_000545"
     CDS             4814..5521
                     /locus_tag="VQ514_000545"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HET2499347.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIAGDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQELK"
     gene            5723..9448
                     /locus_tag="VQ514_000546"
     CDS             5723..9448
                     /locus_tag="VQ514_000546"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HET2499348.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLAEDLFAPAEKHIFSEQGTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITTGIAKQEFS
                     ESDKTIPKLTFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDTFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTIFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANSTSPTLFYNDANQHVTKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            9541..10983
                     /locus_tag="VQ514_000547"
     CDS             9541..10983
                     /locus_tag="VQ514_000547"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HET2499349.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            11020..12042
                     /locus_tag="VQ514_000548"
     CDS             11020..12042
                     /locus_tag="VQ514_000548"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HET2499350.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAISPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            12039..12584
                     /gene="purN"
                     /locus_tag="VQ514_000549"
     CDS             12039..12584
                     /gene="purN"
                     /locus_tag="VQ514_000549"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HET2499351.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKADYEAALVELLEEHQIALVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            12668..13177
                     /locus_tag="VQ514_000550"
     CDS             12668..13177
                     /locus_tag="VQ514_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET2499352.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLIFFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            13202..14749
                     /gene="purH"
                     /locus_tag="VQ514_000551"
     CDS             13202..14749
                     /gene="purH"
                     /locus_tag="VQ514_000551"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HET2499353.1"
                     /translation="MTKRVLISVSDKADIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYDDAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGENKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLDGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
     gene            14871..16133
                     /gene="purD"
                     /locus_tag="VQ514_000552"
     CDS             14871..16133
                     /gene="purD"
                     /locus_tag="VQ514_000552"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HET2499354.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVSETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFFEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGMIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNITWTDKGVTLGVVVA
                     SKGYPLNYSKGVELPVKTDGDIITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELSQQKIEGLFYRTDIGSKAIK"
     gene            16536..17024
                     /gene="purE"
                     /locus_tag="VQ514_000553"
     CDS             16536..17024
                     /gene="purE"
                     /locus_tag="VQ514_000553"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HET2499355.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            17011..18102
                     /gene="purK"
                     /locus_tag="VQ514_000554"
     CDS             17011..18102
                     /gene="purK"
                     /locus_tag="VQ514_000554"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HET2499356.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLADKAKAMAVRIAEQLNLSGTFCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEQYVTENSSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            18112..18339
                     /locus_tag="VQ514_000555"
     CDS             18112..18339
                     /locus_tag="VQ514_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HET2499357.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIEKEEFE"
     gene            18402..19700
                     /gene="purB"
                     /locus_tag="VQ514_000556"
     CDS             18402..19700
                     /gene="purB"
                     /locus_tag="VQ514_000556"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HET2499358.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(19755..23693)
                     /gene="strH"
                     /locus_tag="VQ514_000557"
     CDS             complement(19755..23693)
                     /gene="strH"
                     /locus_tag="VQ514_000557"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HET2499359.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKAEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEDDKPETEAGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKESARTVNLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGSMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQTLNEVPKDLNRY
                     TAESVAAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLNQLKRIVDKASELGYSDVHLLLGNDGLRFLLDDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYILGQKPEDGGG
                     FLKKAIENTGKTPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQKEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEIPSQPAPSTAPA
                     EESKALPQDPAPVVIEKKLPETGTHDSAGLVVAGLMATLAAYGLTKRKED"
     gene            complement(24008..24724)
                     /locus_tag="VQ514_000558"
     CDS             complement(24008..24724)
                     /locus_tag="VQ514_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET2499360.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
     gene            25072..26859
                     /locus_tag="VQ514_000559"
     CDS             25072..26859
                     /locus_tag="VQ514_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HET2499361.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPVYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            26856..27332
                     /locus_tag="VQ514_000560"
     CDS             26856..27332
                     /locus_tag="VQ514_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HET2499362.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            27360..28265
                     /locus_tag="VQ514_000561"
     CDS             27360..28265
                     /locus_tag="VQ514_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HET2499363.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            28252..29067
                     /locus_tag="VQ514_000562"
     CDS             28252..29067
                     /locus_tag="VQ514_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET2499364.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            29074..29478
                     /locus_tag="VQ514_000563"
     CDS             29074..29478
                     /locus_tag="VQ514_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2499365.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
BASE COUNT         8123 a         5759 c         6953 g         8679 t
ORIGIN      
        1 gtaaaaaagt gattagaaaa catctttttt aaaaatagag atgattttga aacaaaaaag
       61 ctaattcaag acgtttcgat gccaattcaa gatttggatg aaaaaaatta atagatactg
      121 ttatactaaa cttgtcaagt ttgtaacaag acaaatatta aaaataaaaa agaggtattc
      181 gttatgaata caaaaaagat gtcacaattt gaaattatgg atactgagat gcttgcttgc
      241 gttgaaggtg gcggatgcaa ttggggagat tttgccaaag caggtgttgg aggaggagca
      301 gtacgaggtc ttcagctagg aattaaaaca agaacatggc aaggtgcagc aactggtgct
      361 gcgggaggag ctatacttgg aggtgtggcc tatgcagcga catgttggtg gtaattatgg
      421 attttaaaag ttttattatt ggtttagtag ttggtatatt tggtccttat atggatgatt
      481 taattagaaa aaaattttta aagtcttcgg agaagaaaac agaaaaatct gttaaaaaat
      541 aatcaaaact ataaatgatg aatctgaatc aaaattattt tgcgcatgta aagaggagtc
      601 ttatagtaac gagtcaaaaa aggagtaact atgaatcgta atttagaacg gtgttatcta
      661 ttctgactag gaatagatca taccagaggt agcttagaaa tagcagggac attagaaatt
      721 gaagtaataa ataggatgtc gtaagtgtta ctatcaatga tttatttgtt tcaagcttgc
      781 ctagggtgac agtaaaaaat caatttcctt tcaatagcat atttttagtg ggcaggactc
      841 ttgttctgcc tattttttta tccaaaaagt gcagttggga gggagatagg ctcatttggg
      901 aaggaagtcc agtttttgtt tagtgattgg ggtaagatag ttgttatcag atgagtttat
      961 actcttcgaa aatcaaattc aaaccacgtc aacgtcgcct tgccgtatat atgtgactga
     1021 cttcgtcagt cctatctaca acctcaaaac agtgttttga gcagcctgcg gctagtttcc
     1081 tagtttgctc tttgattttc attgagtatt agggaaaagg agatgaatat gaaatttggg
     1141 aaacgtcact atcgtccgca agtggatcag atggactgcg gtgtagcttc attagccatg
     1201 gtttttggct actatggtag ttattatttt ttggctcact tgcgagaatt ggctaagacg
     1261 accatggatg ggacgacggc tttgggcttg gtcaaggtgg cagaggagat tggttttgag
     1321 acgcgagcca ttaaggcgga tatgacgctt tttgacttgc cggatttgac ttttcctttt
     1381 gttgcccatg tgcttaagga agggaaattg ctccactact atgtggtgac tgggcaggat
     1441 aaggatagca ttcatattgc cgatccagat cccggggtga agttgactaa actgccacgt
     1501 gagcgttttg aggaagaatg gacaggagtg actcttttta tggcacctag tccagactat
     1561 aagccttata aggaacaaaa aaatggtctg ctctctttta tccctatatt agtgaagcag
     1621 cgtggcttga ttgccaatat cgttttggca acactcttgg taaccgggat taacattgtg
     1681 ggttcttatt atctgcagtc tatcattgat acctatgtgc cagatcagat gcgttcgaca
     1741 ctagggatta tttctattgg gctagtcatc gtctacatcc tccagcaaat cttgtcttac
     1801 gctcaggagt atctcttgct tgttttgggg caacgcttgt cgattgacgt gattttgtcc
     1861 tatatcaagc atgtttttca cctccctatg tccttctttg cgacacgcag gacaggggag
     1921 atcgtgtctc gttttacaga tgctaacagt atcatcgatg cgctggcttc gaccatcctt
     1981 tcgattttcc tagatgtgtc aacggttgtc attatttccc ttgttctatt ttcacaaaat
     2041 accaatctct ttttcatgac tttattggcg cttcctatct acacagtgat tatctttgcc
     2101 tttatgaagc cgtttgaaaa gatgaatcgg gataccatgg aagccaatgc ggttctgtct
     2161 tcttctatca ttgaggacat caacggtatt gagactatca agtccttgac cagtgaaagt
     2221 cagcgttacc aaaaaattga caaggaattt gtggattatc tgaagaaatc ctttacctat
     2281 agtcgagcag agagtcagca aaaggctctg aaaaaggttg cccatctctt gcttaatgtc
     2341 ggcattctct ggatgggggc tgttctggtc atggatggca agatgagttt ggggcagttg
     2401 attacctata ataccttgct ggtttacttt accaatcctt tggaaaatat catcaatctg
     2461 caaaccaagc ttcagacagc gcaggttgcc aataaccgtc taaatgaagt gtatctagta
     2521 gcttctgagt ttgaggagaa gaaaacagtt gaggatttga gcttgatgaa gggagatatg
     2581 accttcaagc aggttcatta caagtatggc tatggtcgag acgtcttgtc ggatatcaat
     2641 ttaaccgttc cccaagggtc taaggtggct tttgtgggga tttcagggtc aggtaagacg
     2701 actttggcca agatgatggt taatttttac gacccaagtc aaggggagat tagtctgggt
     2761 ggtgtcaatc tcaatcagat tgataaaaaa gccctgcgcc agtacatcaa ctatctgcct
     2821 caacagccct atgtctttaa cggaacgatt ttggagaatc ttcttttggg agccaaggag
     2881 gggacgacac aggaagatat cttacgggcg gtcgaattgg cagagattcg agaggatatc
     2941 gagcgcatgc cactgaatta tcagacagaa ttgacttcgg atggggcagg gatttcaggt
     3001 ggtcaacgtc agagaatcgc tttggcgcgt gctctcttga cagatgcgcc ggtcttgatt
     3061 ttggatgagg cgactagcag tttggatatt ttgacagaga agcggattgt cgataatctc
     3121 atggctttgg acaagacctt gattttcatt gctcaccgct tgactattgc tgagcggaca
     3181 gagaaggtgg ttgtcttgga tcagggcaag attgtcgaag aaggaaagca tgctgatttg
     3241 cttgcacagg gaggctttta cgcccatttg gtcaatagct agaaagagga gaggatgaaa
     3301 ccagaatttt tagaaagtgc ggagttttat aatcgtcgtt accataattt ttccagtagt
     3361 gtgattgtac ccatggccct tctgctcgtg tttttacttg gctttgcaac tgttgcagag
     3421 aaggagatga gtttgtccac tagagctact gtcgaaccca gtcgtatcct tgcaaatatc
     3481 cagtcaacta gcaacaatcg tattcttgtc aatcatttgg aagaaaataa gctggttaag
     3541 aagggggatc tcttggttca ataccaagaa ggggcagagg gtgtccaagc ggagtcctat
     3601 gccagtcagt tggacatgct aaaggatcaa aaaaagcaat tggagtatct gcaaaagagc
     3661 ctgcaagaag gggagaacca ctttccagag gaggataagt ttggctacca agccaccttt
     3721 cgcgactaca tcagtcaagc aggcagtctt agggctagta catcgcaaca aaatgagacc
     3781 atcgcgtccc agaatgcagc agctagccaa acccaagccg aaatcggcaa cctcatcagt
     3841 caaacagagg ctaaaattcg cgattaccag acagctaagt cagctatgga aacaggtgct
     3901 tccttggcca gtcagaatct agcctactct ctttaccagt cctacaagtc tcagggcgag
     3961 gaaaatcccc aaactaaggt tcaggcagtt gcacaggttg aagcacagat ttctcagtta
     4021 gaatctagtc ttgctactta ccgtgtccag tatgcaggtt caggtaccca gcaagcctat
     4081 gcgtcagggt taagcagtca attggaatcc cttaaatccc aacatttggc aaaggttggt
     4141 caggaattga cccttctagc ccagaaaatc ttggaggcag agtcaggtaa gaaggtacag
     4201 ggaaatcttt tagacaaggg gaaaattacg gcgagtgagg atggggtgct tcatcttaat
     4261 cctgagacca gtgattctag catggttgca gaaggtgccc tactagccca actttatcca
     4321 tctttggaaa gagaagggaa agccaaactc acagcctatc taagttcaaa agatgtagca
     4381 agaatcaagg tcggtgattc tgttcgctat actacgactc atgatgccgg gaatcaactt
     4441 ttcctagatt ctactattac aagtattgat gcgacagcta ctaagactga gaaagggaat
     4501 ttctttaaaa tcgaggcgga gactaatcta acttcggagc aggctgaaaa acttaggtac
     4561 ggggtggaag gccgcttgca gatgattacg ggcaagaaaa gttatctacg ttattatttg
     4621 gatcaatttt tgaacaaaga gtaatgttcg tgtttttaga gttaaataat ttttaaactg
     4681 tgagaaagat tcttcttgca gttttttctt tacaattttt aaaaaacatc tgctatttat
     4741 tcggttaaat tcttgtgttt tttggttttt tgtggtaaaa tgtgctcaag taatacgaaa
     4801 ggcgaacttt aaaatgtcaa aacaattgat ctattcggga aaagctaaag atatctatac
     4861 aactgaggat gaaaatctta ttatttcaac ttacaaggac caggcgactg ctttcaacgg
     4921 tgtcaagaag gagcagattg caggtaaggg agtcttgaat aatcagatct catcttttat
     4981 ttttgagaaa ttaaatgcgg ctggtgtggc gactcacttt gtggagaaac tttcagacac
     5041 ggaacaactc aataaaaagg ttaagattat tcctttggaa gtcgtgctcc gcaactatac
     5101 tgctggttcc ttttcaaaac gttttggtgt ggatgaggga atcgccttgg agactccgat
     5161 tgtcgaattt tactacaaaa atgatgattt ggatgatcca tttatcaatg atgagcatgt
     5221 gaaattccta cagattgcgg gtgaccagca gattgcctac ttgaaggaag aaacgcgtcg
     5281 tatcaatgaa ctattgaaag tctggtttgc tgagattggg cttaaattga ttgactttaa
     5341 gctagagttc ggttttgaca aggatggcaa gattatcttg gcagacgaat tttcaccaga
     5401 taactgccgc ttgtgggacg ctgatggcaa ccacatggat aaggatgttt tccgtagagg
     5461 attgggagaa ctaaccgacg tttatgagat tgtttgggaa aagttgcagg aattgaaata
     5521 atctgtttgc aacggaaaac cttcgtctct caactaaaag gactcaggct gaaaaggtcc
     5581 cccagacctt ttcactctgt agagaactag gtgaactaac agatgtttac gaaattgtct
     5641 gggaaaagtt gcagggttta aaataacaac ctcaaggctg tttgggaata ttgcaagagc
     5701 tgaaataaag gaataagaat tgatggataa acgtattttt gttgaaaaaa aggctgattt
     5761 tcaggtcaag tcagagagtt tggttagaga gctccagcac aacttgggac tgtcaagctt
     5821 gaaaagtatt cgtattgtgc aagtatatga tgtatttgac ttggctgagg acttgtttgc
     5881 acctgcagag aagcacattt tctctgagca gggaaccgac catgttttag atgaagtatc
     5941 tgtgcaggcg gatcttgcta actatgcttt ctttgccatt gaaagtctgc cagggcagtt
     6001 tgaccagcgt gcagcttcgt cacaggaagc cttgcttttg ttgggaagtt cgagtgacgt
     6061 gacagtcaac acagcccaac tttacttggt gaataaagat attgatgcga ctgagttgga
     6121 agctgtcaaa aactacctgc tcaatccagt tgattctcgt ttcaaggata tcacgacagg
     6181 gattgccaag caggagtttt cagagtcaga caagaccatt ccaaaattga ctttctttga
     6241 aagctataca gcagaagact ttgctcgcta caaggccgag caagggatgg ccatggaagt
     6301 ggatgatttg ctctttatcc aagactactt taagtcaatc gggcgcgtgc caactgagac
     6361 tgaactcaag gttttggaca cttactggtc tgaccactgc cgtcatacga cttttgagac
     6421 agagttgaaa cacatcgact tttcagcttc taaatttcaa aagcaattgc agtcaaccta
     6481 tgacaagtat attgccatgc gcgaggagtt aggtcggtct gaaaaaccac aaaccttgat
     6541 ggatatggcg actattttcg gtcgttatga gcgtgctaac ggacgattgg atgatatgga
     6601 agtctctgac gaaatcaatg cctgctcagt tgaaattgaa gtggatgttg atggtgtcaa
     6661 ggaaccttgg ctcctcatgt ttaaaaatga aacccacaac catccaacag aaattgagcc
     6721 atttggtgga gcggctacct gtatcggtgg agctattcgt gatccgttgt caggccgttc
     6781 ctatgtttac caagccatgc gtatttcagg tgctggtgat attacagcac cgatttcgga
     6841 aactcgtgct gggaaattgc cacaacaagt catttctaaa acagcagctc atggttattc
     6901 ttcatatggt aaccagattg ggcttgccac aacctacgtt cgtgaatact tccacccagg
     6961 ctttgtagct aaacgtatgg aacttggtgc cgttgttggt gcggctccca agggcaatgt
     7021 tgtccgtgaa aaacctgaag caggtgatgt gatcatcctt ctcggaggca aaacaggtcg
     7081 tgatggtgtc ggtggtgcga cgggctcttc taaggttcaa acagttgagt ctgtagagac
     7141 tgctggtgct gaggttcaaa aaggaaatgc catcgaagaa cgcaagattc agcgcctctt
     7201 ccgtaatggc aatgtcactc gtctgatcaa gaagtccaat gactttgggg caggcggcgt
     7261 ctgtgtggct atcggtgaat tggcagacgg tcttgaaatc gacctcaaca aggtgcctct
     7321 taaataccag ggcttgaatg gtacagaaat tgccatctct gaatcacaag aacggatggc
     7381 ggtcgtggtt cgtcctgaag atgtggatac cttcgttgcc gaatgtaaca aagaaaatat
     7441 tgatgctgtt gtggtggcga cagtaactga aaaaccaaat cttgtcatgc actggaatgg
     7501 tgagacaatc gttgacttgg agcgtcgttt ccttgacacc aatggtgtgc gcgtggttgt
     7561 cgatgccaaa gttgtggaca aggatgtcaa actcccagaa gagcgtcaaa catctgctga
     7621 aacactggaa tcagataccc ttacggttct atctgacctc aaccatgcaa gtcaaaaagg
     7681 attacagact atctttgact gctctgttgg acgctcaacg gttaatcacc cacttggtgg
     7741 tcgttaccaa ctcacaccaa ctgaggcatc tgtgcagaaa ttgccagttc aacacggtgt
     7801 gactcatact gcgtcggtca ttgctcaagg tttcaaccca tatgtagctg aatggtctcc
     7861 ataccacggt gctgcttatg cggttatcga agcaactgct cgtttggtgg ctgctggtgc
     7921 caactggtct aaggctcgtt tctcttacca agaatatttc gagcggatgg acaagcaagc
     7981 agagcgtttc ggtcagccag tagctgctct tctaggttct attgaagcac aaattcagct
     8041 tggcttgcca tctatcggtg gtaaggactc catgtctggt acctttgaag aattgaccgt
     8101 tccgccaacc ttggttgcct ttggggtgac gacggcagat agccgtaagg tgctctctcc
     8161 agaatttaaa gctgttgggg aaaatatcta ctacatccca ggtcaagccc tctctgcaga
     8221 gattgatttt gacttgatta agaaaaattt tgctcagttt gaagcaagcc aagctgacca
     8281 taaagtgacg tctgcatcag ctgtcaaata cggtggtgta gttgaaagtt tggctcttgc
     8341 tacctttgga aactatattg gtgcagaggt gaccttgcct gaacttaaaa cagctttgac
     8401 agctcaatta ggcggctttg tcttcacatc tcctgaagaa attgctggag tagagaagat
     8461 tggacaaacg aaagcagact ttacactgac tgtcaacggt gtgaagctag atggacacaa
     8521 gcttgacagt gcatttcaag ggacattgga agaagtttac ccaacagaat ttacccaagc
     8581 gaaagaacta gaagaagtac cagctgtggc atcagatgtt gtgattaaag ccaaagaaaa
     8641 ggttgaaaaa cctgtggttt acatcccagt ctttccagga accaactcag aatatgattc
     8701 agctaaggcc ttcgaaaaag aaggtgcaga ggtcaatttg gtgccattcg tgaccttgaa
     8761 tgaagaagct attgtcaagt cagttgaaac tatggttgac aatatcgaca agactaatat
     8821 tctcttcttt gccggtggat tctcggctgc ggatgaacca gatggttcag ctaagtttat
     8881 cgtcaatatc ctgcttaatg aaaaagtgcg tgtggctatt gatagcttta tcgcccgtgg
     8941 tggcttgatt atcggtattt gtaatggatt ccaagcctta gtcaaatcgg gtctcctacc
     9001 atacggaaac tttgaagatg ctaacagtac tagtccaacc ctcttctaca atgatgccaa
     9061 ccaacacgtg accaagatgg tggaaactcg cattgccaat accaactcac catggttggc
     9121 tggtgtgcaa gtgggcgata tccacgctat tcctgtttcg cacggtgaag ggaagtttgt
     9181 cgtgacggct gaggaatttg cagagctccg tgacaatgga caaattttca gccaatacgt
     9241 tgactttaac ggtaaaccaa gtatggattc taagtacaat ccgaatggtt ctgtccatgc
     9301 catcgaagga attaccagca agaatggtca aatcatcggt aagatgggcc actcagaacg
     9361 ttatgaggat ggtcttttcc aaaatatccc aggcaataaa gaccaacacc tgttcgcatc
     9421 agcggttaaa catttcactg gaaaataaga cttacagatt ttctaataga tagtatcagt
     9481 aatgtaaaag tcatgtaaat ctagctcttg atgattacaa atgaaaatta ggtataaaaa
     9541 atgacatacg aagtaaaatc tcttaatgaa gaatgtggtg ttttcggtat ttggggacat
     9601 ccagatgctg ctaagttgac ctattttgga ctccacagtc ttcaacaccg tggtcaggag
     9661 ggggcaggaa tcctctccaa tgatcaagga caactgaagc gccatcgtga catggggctt
     9721 ttatcagaag ttttcagaaa tccagccaat ttggataaat tgacaggagc tggtgcgatt
     9781 gggcatgtgc gttatgcgac tgctggcgaa gcttctgttg ataacatcca gcccttcctc
     9841 ttccgttttc acgatatgca gtttggcttg gctcataatg gaaatctgac caatgcagcc
     9901 tctctcaaga aagaactgga acaaagagga gcaattttca gcgcgacttc ggactctgaa
     9961 atcttggctc acctcattcg tcgtagtcat aatcctagcc tgatgggcaa aatcaaggaa
    10021 gcgctcagcc ttgtcaaagg tggttttgcc tatatcttgc tgtttgagga caagttgatt
    10081 gcggctcttg accctaatgg ctttcgtccg ctttctatcg ggaaaatggc caacggagcg
    10141 gtggttgttt cctctgaaac ctgtgctttt gaggtcattg gtgccgagtg gattcgtgat
    10201 ttgaagccag gtgagattgt gatcattgat gacgagggca ttcagtatga cagctataca
    10261 gatgatactc agttggcgat ttgttctatg gagtatatct actttgctcg ccctgattct
    10321 aatatccacg gtgtcaatgt ccatacggca cgtaagagaa tgggagcgca attggcgcga
    10381 gaatttaagc atgaggcaga tattgtagtt ggtgtgccca attcttccct aagcgcggct
    10441 atgggatttg cggaagaatc aggcttacca aatgaaatgg gtctgatcaa aaaccaatac
    10501 acccagcgaa cttttatcca accgactcaa gaattgcggg agcaaggagt gcggatgaaa
    10561 ctgtctgctg tttcgggtgt tgtcaaaggc aaacgtgtgg tcatggtgga tgattccatt
    10621 gtacgtggaa caacctctcg tcgtatcgtt cagctcttga aagaagcggg tgcgactgag
    10681 gttcacgttg ccattggaag tcctgcacta gcgtatccat gtttctacgg gattgatatc
    10741 cagacccgtc aggagctgat tgcagccaat catacggtcg aagaaactcg ccaaatcatt
    10801 ggtgcggaca gtctgactta tctttcaatt gatggcttga ttgagtcgat tggtatcgaa
    10861 acagatgcgc cgaacggtgg tctctgtgtc gcttactttg acggtgacta cccaacgcct
    10921 ctctatgact acgaagaaga ctatcgtaga agtttggaag aaaagaccag tttttacaag
    10981 taggcgacag attctccatt aaagaaaagg aaaaaacaaa tgacaaataa aaatgcatat
    11041 gcccagtcgg gtgtggatgt tgaagcgggt tatgaagttg ttgaacggat taaaaagcac
    11101 gtggcccgta cggagcgtgc aggtgtcatg ggagctcttg gtggctttgg tggtatgttt
    11161 gacctttcca agactggggt taaagaaccc gtcttgattt cagggactga cggtgtcgga
    11221 accaagctca tgttggctat caagtacgac aagcacgata ccatcgggca ggactgtgtg
    11281 gccatgtgtg tcaacgacat cattgctgca ggtgcggaac ccctctattt tctcgactac
    11341 gtagcgacag ggaagaatga accagctaag ctagaacaag tggttgctgg tgtggcagaa
    11401 ggttgtgtgc aggctggtgc tgccctcatc ggtggggaaa cggctgaaat gccgggcatg
    11461 tacggcgaag acgactatga cttggctggt tttgcggtcg gtgtggctga aaaatctcaa
    11521 atcattgacg gttcaaaggt ggtagaggga gatgttcttc tcggacttgc ttcaagtggg
    11581 attcactcaa atggttactc tttggttcgt cgtgtctttg cggattacac aggtgaggaa
    11641 gtcctaccag aattggaagg caagaaactt aaggaagttc tacttgagcc gactcgtatc
    11701 tatgtcaagg ctgtcttgcc gctcatcaaa gaagagttgg tcaacggcat tgcccacatc
    11761 acaggtggtg gctttatcga aaatgtccct cgtatgtttg cagatgacct agctgctgaa
    11821 attgatgaaa gcaaagttcc agtgcttcca attttcaaag cccttgaaaa atatggtcag
    11881 attaaacacg aagaaatgtt tgaaatcttc aatatgggtg tgggacttat gttggcgatc
    11941 agccctgaaa atgtagagcg tgtaaaagaa ttgttggatg aagcagtcta tgaaattggt
    12001 cgcatcgtca agaaagaaaa cgaaagtgtc attatcaaat gaaaaaaata gcggtttttg
    12061 cctctggtaa tggctcaaat tttcaggtga ttgccgaaga atttccagtg gagtttgtct
    12121 tttcagacca tcgtgatgcc tatgtgcttg agcgtgcaaa gcagctcggc gttctgtcct
    12181 atgcttttga actcaaggag tttgagagca aggcagacta cgaagcagcc cttgtcgaac
    12241 tcttggaaga acaccagatt gccttggttt gcctagcagg ctacatgaaa atcgttggac
    12301 caaccttatt gtcggcttat gaaggtcgga ttgtcaacat tcatccagcc tacttgccag
    12361 aatttccagg agctcatggg attgaggatg cttggaatgc tggcgtgggt cagtctggtg
    12421 tgaccattca ctgggtggat tcgggtgtgg atacaggcca ggtcatcaaa caggttcgtg
    12481 tgccacgact agctgatgat accattgaca gatttgaagc tcgcatccat gaagcagagt
    12541 acaggttgta tccggaagta gtgaaggctc tatttacaga ttgacttttt gatgattcat
    12601 atgatatctt tgattttaaa ttggagtcag tgtttgttga agacggcttc aaacggaggt
    12661 atttgtaatg ttagaatcta aaaaaacaac tcgatatgta ttttatgtct atctgatgtt
    12721 attaacttgg ggaatcttat ttaagtttga aacaaatcct gaatttatag catttttctt
    12781 agctccaagg tatatcaatt ggattccatt ttcagaacca ctaatagtcg atggaaaaat
    12841 tgtttttgct gaaatgttat ttaatctgat tttctttatt ccattaggtg tttgtttccc
    12901 tttgataaaa actaatttat ctagtttaag aatagtcggg acaggtttct tgattagttt
    12961 attgtttgag tgcttacagt atattttagc aataggtata acagatataa cggatttgac
    13021 tttaaatacg ctaggtgtct gtgtaggctt actgatttat caaattttta taagagtgtt
    13081 caaatcacag actagaaaat ggatcaatat cttaggtatg cttagccttg gttttgctta
    13141 tcttgtttta ctgttactgc atttaattgg tgtttaacta atgattaaaa aggagaatat
    13201 aatgactaaa cgcgtcttaa tcagcgtctc agacaaagcg gacattgttg aatttgccca
    13261 agaactcaaa aaacttggtt gggagattat ctcgacaggt ggaactaagg ttgcccttga
    13321 taatgctggg gtggatacca ttgctatcga tgatgtgact ggtttcccag aaatgatgga
    13381 cggtcgtgtg aagaccctcc acccaaatat ccacggaggg cttctcgctc gtcgtgactt
    13441 ggatagccac ttggaagcgg ctaaggacaa caagattgag ctcattgacc ttgtggtggt
    13501 caacctttac ccatttaagg aaactatcct taaaccagat gtgacttatg atgatgcagt
    13561 tgaaaatatc gatattggtg ggccatctat gcttcgttca gcagcgaaaa atcatgccag
    13621 tgttacagtt gtggtagatc ctgctgacta cgctgtggtt ttggacgaat tggcagcaaa
    13681 cggcgaaacc tcttatgaaa ctcgccaacg tttagcagcc aaagtatttc gtcacacagc
    13741 ggcttatgac gccttgattg cagaatactt cacagctcaa gtgggtgaaa acaagcctga
    13801 aaaactcact ttgacttatg acctcaagca accaatgcgt tacggtgaga atcctcaaca
    13861 agacgcggac ttttaccaga aagctttgcc tacagactac tccattgctt cagccaaaca
    13921 gctcaacggg aaagaattgt catttaataa tatccgtgat gcagatgctg ctatccgtat
    13981 catccgtgac ttcaaagata gtccaaccgt tgtggctctc aaacacatga atccatgtgg
    14041 aattggtcaa gctgatgaca tcgagactgc ttgggactac gcttatgagt ctgacccagt
    14101 gtctatcttt ggtgggattg tcgtcctcaa ccgtgaggtg gatgctgcga cagctgagaa
    14161 gatgcacggc gttttcctcg aaatcatcat tgcaccaagc tatacggatg aagcgctagc
    14221 cattttgatc aataaaaaga aaaacttgcg tatccttgcc ttgccattta atgctcaaga
    14281 ggctagcgaa gtggaagcag aatacacagg tgtagtcggt ggacttctcg tgcaaaatca
    14341 agacgtggtc aaggaaagcc cagctgactg gcaagtggtg actaaacgtc agccaactga
    14401 gacagaagcg actgctcttg agttcgcttg gaaggctatc aagtacgtca aatcaaatgg
    14461 tattatcgtg accaacgacc acatgacact tggtgttggt ccaggtcaaa ccaaccgtgt
    14521 ggcttctgtt cgccttgcca ttgaccaagc caaagatcgt ctggacgggg cggtccttgc
    14581 ttcagatgcc ttcttcccat ttgcggataa cgtggaagaa atcgccaaag caggaattaa
    14641 ggccatcatc cagcctggtg gctctgtccg tgaccaagaa tccatcgaag cagcggataa
    14701 atacggcttg actatggtct ttacaggtgt gagacatttt agacattaag aagataaaag
    14761 ggaagaaaac agtttctttc cttttttggc ttaaaatgct aactgaaaca agattaaaac
    14821 gaactttttt gatataatgt tggtaaataa ttcgcaaaag aggttgagga atgaaactgc
    14881 ttgttgtcgg ttctggtggt cgtgagcatg cgattgctaa aaagttactt gaatcaaaag
    14941 acgtggaaaa agtctttgta gctcctggga atgatgggat gactctggat ggtttggaat
    15001 tggtaaatat ctctatttcc gaacattcta aattgattga cttcgcaaag accaatgatg
    15061 ttgcttggac ctttatcggt ccagatgatg cccttgctgc tggtatcgtg gatgatttta
    15121 accaagctgg gcttaaggcc tttggtccga ctagggctgc agcggagctg gagtggtcca
    15181 aggattttgc caaggaaatc atggtcaaat atggtgtttc gacagcagcc tatggcacat
    15241 tttcagattt cgaggaagca aaggcctata tcgaaaagca tggtgcgcct atcgtagtca
    15301 aggcggatgg cttggcgctt gggaagggtg tcgtcgtttc tgagacggtt gagcaagcgg
    15361 tcgaagccgc tcatgagatg cttttggaca ataaatttgg tgactcaggt gcgcgcgtgg
    15421 ttattgagga attctttgaa ggagaggaat tttcactctt tgcctttgtc aatggtgata
    15481 agttctacat catgccaacg gctcaggacc acaaacgtgc ctatgatggc gacaaagggc
    15541 ctaacacggg tggtatgggt gcctatgcgc cagttccaca cttaccacag agtgtagttg
    15601 atacagcggt tgacaccatt gtcaagccag ttctagaagg gatgattaaa gaaggtcgcc
    15661 cttatctggg agttctttac gcagggctta tcctgacagc tgatggaccg aaagtcattg
    15721 agttcaacgc tcggttcgga gatccagaaa ctcagattat cttgcctcgc ttaacctctg
    15781 actttgctca aaatatcaca gatatcctgg atagcaagga gccaaatatc acgtggacgg
    15841 acaagggtgt gactctgggt gtggttgtcg catccaaggg ctacccgcta aattattcaa
    15901 agggtgttga gttgccagtc aaaaccgatg gcgacatcat tacctactat gcaggggcta
    15961 agtttgcgga aaatagcaga gcactgctct caaacggcgg acgagtttat atgctcgtta
    16021 ccacagcaga taccgtcaaa gaagcccaag ccagcatcta ccaagaacta tcccaacaaa
    16081 aaatagaagg actcttctac cgaacagata tcggaagcaa ggcaattaag taaagatata
    16141 agaataacgc gccgtagtcg ccaaacacga taatggtcgt cgtggtgaaa agaccagaac
    16201 agtgaatgtt ctggtcaggg ggaaacttgg agaccgtagg ctcaaagttt aggaatgaaa
    16261 ccgaaggttt gcttccgcct ccatcaccta agaccattat caaaaagaaa aataaaaatt
    16321 cacaaaatac gttaatgatc gtatggtttg cgagcgttag cgagctaata tagaacaatc
    16381 accgccgttg tgaaagaacg attggatgat aatccaatcg ttcagggaaa ttggaagacc
    16441 ttgggtttcc aatttaggca tgagacacct ttggtggctg ctgccgtccc tcacaagcta
    16501 aggtgattgt tgaaaaagag gaaaaaggag aagaaatgaa accagtaatt tccatcatca
    16561 tgggctcaaa atccgactgg gcaaccatgc aaaaaacagc agaagtccta gaccgcttcg
    16621 gtgtagccta cgaaaagaaa gttgtttccg cacaccgcac accagacctc atgttcaaac
    16681 atgcagaaga agcccgtagt cgtggcatca agatcatcat cgcaggtgct ggtggcgcag
    16741 cgcatttgcc aggcatggta gctgccaaaa caacccttcc agtcattggt gtgccagtca
    16801 agtctcgtgc tcttagtgga gtggattcac tctattctat cgttcagatg ccgggtgggg
    16861 tgcctgttgc gaccatggct atcggtgaag ctggagcgac taacgcagct ctctttgccc
    16921 tccgtctcct ctctgtagaa gataagtcca ttgcggatgc acttgccaac tttgctgaag
    16981 aacaaggaaa aatcgcagag gagtcgtcaa atgagctcat ctaaaacaat cggaattatc
    17041 ggtggcggtc aactgggtca gatgatggcc atttctgcta tctacatggg ccacaaggtt
    17101 atcgcgctgg atcctgcggc ggattgcccg gcctctcgcg tggcggaaat cattgtggca
    17161 ccttataacg atgtggacgc cctccgtcag ttggcagacc gttgcgatgt cctcacttat
    17221 gagtttgaaa atgtcgacgc tgacggtttg gatgccgtta tcaaggatgg acaactccct
    17281 caaggaacag atctgctccg catttcgcaa aatcgtattt ttgaaaagga ctttttgtca
    17341 aacaaggctc aagtcactgt ggcaccctac aaggtcgtga cttctagcct agacttggca
    17401 gatatcgact tgtcgaaaaa ctatgtcctc aagactgcga ctggtggcta cgatggtcat
    17461 ggacaaaagg ttattcgttc agaagcagac ttggaagcag cctatgcgct agcagactca
    17521 gcagactgcg tcttggaaga atttgtcaac tttgaccttg agatttctgt catcgtgtca
    17581 ggaaatggca aggaggtgac gtttttccca gttcaggaaa atatccaccg caacaatatc
    17641 ctgtctaaga ccatcgtacc agcccgcatt tctgaaagtc tagcagacaa ggctaaagct
    17701 atggcagtgc gaatcgcaga acaactcaac ttgtctggaa ctttctgtgt ggaaatgttt
    17761 gcgactgatg atgacgttat tgtcaatgaa atcgccccac gaccacataa ctctgggcac
    17821 tattctattg aagcctgtga tttctctcag tttgacaccc atattctggg tgttctcgga
    17881 gcaccattac cagtcatcaa actccatgcg ccagccgtta tgcttaatgt cctcggtcag
    17941 catgtcgagg ctgctgagca atatgtgaca gaaaattcaa gcgcccacct ccacatgtat
    18001 ggtaaaatag aagcgaagca caaccgcaag atgggacatg tgaccttgtt tagtgatgta
    18061 ccggatagtg tggatgagct tggggaaggg attgattttt aggacaagtc tatgatacaa
    18121 attatcgtta atacatttat tgaaaaggat aagactggag cagttgttga agtgttgtat
    18181 gccagtgctg accaagataa ggtacaagct aaatatgaag aactagctgc acaatacccc
    18241 gaaaattatt tagctatcta tgatgtaccg ctggatacgg atttgaatac actagatcat
    18301 tacccgtctg tgtttattga aaaagaggag tttgagtaga aatcttggtt tacctagata
    18361 gcttattccc aacagcttaa gaagaaagga aaaattaaca catgatcaac cgttactctc
    18421 gccctgagat ggcgaatatt tggagtgaag aaaataaata ccgtgcttgg cttgaggtgg
    18481 aaatcctctc tgacgaggca tgggctgagt tgggggaaat ccctaaggaa gatgtggctt
    18541 tgattcgcaa gaaggcggac tttgacatcg accgtatttt ggaaattgag caggagacgc
    18601 gccacgatgt ggtggctttc acgcgtgcgg tttctgagac tcttggtgaa gagcgcaagt
    18661 gggttcacta tgggttaact tctaccgacg tggtggatac tgcttatggt tacctctaca
    18721 agcaggccaa cgacatcatc cgtcgtgacc ttgaaaactt cactaatatc atcgctgaca
    18781 aggccaagga gcacaagttc accatcatga tggggcgtac tcatggtgtg cacgctgagc
    18841 cgacaacctt tggtcttaaa ttggcaactt ggtacagcga aatgaaacgc aatatcgagc
    18901 gtttcgagca tgcagctgct ggtgtagaag ctggtaagat ttctggtgcg gttgggaact
    18961 ttgccaatat cccaccattt gtagaggagt atgtctgcga taaacttggc atccgtgccc
    19021 aagaaatctc tacacaagtc cttcctcgtg accttcacgc tgagtacttt gcggttcttg
    19081 ccagcattgc gacttcaatc gaacgtatgg cgactgagat tcgtggtcta caaaaatctg
    19141 agcaacgcga agtagaagag ttctttgcta aagggcaaaa agggtcttca gcaatgcctc
    19201 acaaacgcaa cccaatcggt tctgaaaata tgactggtct ggcgcgtgtc attcgtggtc
    19261 acatgattac ggcttatgaa aacgtcgctc tctggcacga acgcgatatt tctcactcat
    19321 cagctgagcg tatcatcaca ccagatacga ccattttgat tgactacatg ctcaaccgtt
    19381 ttggaaatat cgtcaagaac ttgacagtct tcccagaaaa tatgatccga aacatgaact
    19441 cgacttttgg tcttatcttt agccaacggg ctatgttgac attgattgaa aaaggcatga
    19501 cccgtgagca agcctatgac ttggtgcaac caaaaacagc ctactcttgg gacaaccaag
    19561 tagactttaa accacttctt gaggcagatt cagaagtaac atcacgtctc actcaagaag
    19621 aaatcgatga aatcttcaac ccagtttatt acaccaaacg agtggatgat atctttgaac
    19681 gtcttggact aggtgattaa ttaaaaaata aacagcgagc ttcaatctcg ctgtttattt
    19741 tttatcgaaa agacttagtc ttcttttctt ttagtgagtc cataggctgc tagtgtggcc
    19801 atgagtcctg cgactactag tcctgcagaa tcgtgagttc ctgtttcagg aagttttttc
    19861 tctattacca caggagctgg atcttgagga agagctttgc tttcctcagc aggagcagtt
    19921 gatggagctg gttggcttgg gatttctagt tttggttttt cttcagcaat agcggcttgt
    19981 ccgttttcat cgcctacatg tgttaccata gttccgactt cgactatttg agtaacggct
    20041 tcctgtgcta cgacactatt tacaagtgtt ttcacttcct taccatcggc agaagtgctc
    20101 acagagtaga agttgctacg atgtccattg acgcccttag taatgacttg tgtttttcct
    20161 ttgagtaaga gtggattttc acgagtcact gtggtaaatg gaatttcttc tttttggata
    20221 tccagtctag gttttacctc agtagttggt gcaagaccac tttcatcacc cttgtgagtt
    20281 acaggagcgc caacttcaac cacttggttt ataacttctt tggttacctg gctatcaagg
    20341 actgtttctg ttgtttttcc attttcagtg agtacagaga tgtaatgagt tcgctcacct
    20401 ttgactcctg ctgtgataat attttcctga ccagctggga ggttaggatt ttctttcttg
    20461 ataacttcaa atggaatttc ttcagttctt gtgatgagtt ctggtctggt ttcaacattg
    20521 gcagccactt cattttcatc caggcttcct gaatgagttg cagctggttt gaggccttga
    20581 agagcggctt ttaggttggc tacaagtgtg tcaagctcag cttgtttatt acggttgagg
    20641 ttgtaattta gagctgtttt agctgcgtca agggcctcaa gactttcttt actatatcct
    20701 tctaagtttg taggaatttt agctaattct tcgcggagag cattataatt agcacggaag
    20761 tagtctttgt tgtggtctgc aaaggcagtc atgagttcaa agatttcctc ttccttgtat
    20821 tcagcgcttg gtctatctgc ccagattgaa agcatacttc cgactgttgg aagatctact
    20881 tcaggatatt tggtagaagc tagttgattg aatggtgttt ttccagtatt ctcaatagct
    20941 ttcttgagga aaccaccacc atcttctggt ttttgaccaa gaatgtagta ccagtcaccg
    21001 ttggtattca agaatttata gcctttgctt gctaggtatt gaggtgatgc gaggttatat
    21061 ccccaccagc ctttagacca gtaagaaatc aagacatctt tgtcaaactg aacatcgtcc
    21121 ttgtcttcat agtagaagcc atcgttgaag gccattggtt gaagccctct ttctttggcc
    21181 atagctgcga gggtgttggc atattcggca aatttgccat agagttgata ccacttgagg
    21241 tagtaccagc cttgggcact agtcgcatcg ttggcgtatt cgtcagtacc aaagttgaaa
    21301 atctttgttt tacctgcaaa gaagtccatg tatttaccga tgagggcttt tacaaagttc
    21361 atcgcttctt cgtttttcaa gtccatagtt gtttttgaaa ctttatcaaa gtgggcttga
    21421 ggatttttaa tacctaattt ttccatggca accagcatag catccatgtg acctggactg
    21481 ttaatagctg ggatgagacc gatgtcctta gatttagcgt attcaattag ctctgttact
    21541 tctgcctgtg ttagtgcagt accgtttgga tcgtcgtagt aagctttagt tccttcgata
    21601 atagcttttt taacgtcatc actagcatag gtttttccgt tggcagtaat ggtcatatca
    21661 tcgagtagaa agcgaagtcc gtcatttcct agaaggagat ggacatcaga atatccgagc
    21721 tcactggcct tgtctacgat gcgtttgagc tggttcagag taaagtattt gcgtccagca
    21781 tcgattgaga ttaccttgtt tttggcaagt ttttcaacct cacgtttagc ttcttcttct
    21841 ttttgagctt caggcgtgag ggtcaagttg ttgacagttt cttgaagttt agcaatggct
    21901 tgatcaatcg tatcttgttg ggcacggcta aggttgctat cgagagagcg aatagctttt
    21961 tcagcttctt ttacggccgc gacgctttct gcagtataac ggttcaggtc ttttggtacc
    22021 tcgttaagtg tttgctctgc agattcataa tcagctgcga agtattcagc gttggcattt
    22081 gcaaaatgac gcatgagttt gaagaggcgt gatggtgaat aacgtgcaga tggagtgtca
    22141 gcccaagcag ctaccatact accgatgatt gggatatcag ctccttctgt ttttggtaca
    22201 gaagtgattg gtgtgttttt aataccattg agcccctgat cgagattgta ccagccttgg
    22261 ccatcagcgt ttcgtccaag aacgtagtac caagcatcat tggtattaag gatttggtga
    22321 cctttttcag ctagtagttt agaagaagcg acatcgtagc ctccccaacc accagtccac
    22381 atagaaacga tgatgtcttt gtcaaaacta ccaaagcttg tgtcgctatt gtagtagata
    22441 ccgtcgttaa aggccattgg tttgagaccg tgcgatttta caatacgagc gaggtcattg
    22501 gcgtaggcaa taaatttttc atagcctttt acagggtagc cttcgtttgg atagtattta
    22561 tcagcttgaa gcacactcca acctttagca tctgtcgcat cattggcata ttcatcaagt
    22621 ccgatgttga agatttcagt ctttttcgcg aaataagcag catacttgtc gataagggct
    22681 tttgtaaaag cgacagcttg ttcgttgtca agattgacag tacgggctga ttccttccca
    22741 aaatagctaa agttagggtt ttggattccc aattctttca tggcattgag aatcgcatcc
    22801 atgtgaccag gactatttac tgtcggaatg agaccgatac ctttatcttt ggcatagtta
    22861 atcagatctg tcatttgact ttctgttaag tgattgccgt ttggatcgtt gtaataatca
    22921 tttgtacctt tttcaatggc gcgtttgaca tcgtcactgg cataggtctt gccgttagct
    22981 gtgatgctca tatcgtccaa catgaaacgg agtccatcat ttccgactaa taggtgtaaa
    23041 tcagtgtagc cataatgttt cgctttatcg atgatttcct tgagctgttc tggtgagaaa
    23101 tatttacgtc cagcatcaat agaaacaatt ttctttttcg ctagtttttc atttacagtt
    23161 gcagcacgtt cctttcctgc ctctgtttcc ggtttgtcat cctctgcttt cgcttcatct
    23221 tttttagctg gtttatcctt gtcagtcttg tctgtatttg actctttaga atcaacctct
    23281 ttcgcttctt cctttttagg gctagcttct tctgcctttt tattagcagt ttctttttca
    23341 gcagaagttg gagttaccac ttctgcttta tcactaggag ttgaactaac ttcctcttgt
    23401 ggtttttctt ctgtttttgg aagactagct accttatcag tagctggagt ttctgtttct
    23461 acagtttttg gagcttctgg ttgaagctct gctttaggtg tttcctcagt ccgattttcg
    23521 gatgattgag gggaatcaga aaccgtatgg atggtcggtt ggttttctgt agtagtagga
    23581 gtaactccat cggctgcaac agtctgtgct tggaaggcaa atccaattag aacagaagct
    23641 gctcctacag cgtatttacg aatagaaaaa cgctgttgtt tttcatgttt cattgcaaaa
    23701 cctcctgatt gcattgttat attgatagcg attatataaa tcaacgcctt tattttattt
    23761 cttatattaa cgagagtcaa gaggagatga caaaaaacta taataagtat aaaaaaatat
    23821 aaaatttaaa cttaagattt cagattggtc ggaaaaaata cgtatatata tctagtataa
    23881 tttttggttc tatttctata aaatattcca caaattatag aattttccaa aaataggtaa
    23941 gcgctacctt tttggtgtgg tataataagc atagaaaaag cccaagcgat tagctcaggc
    24001 tttcttctta gtgatcacgg tcacatgaga taaatttaat cttgtagtaa tcagatcgtt
    24061 tgtaagtttc actgtattct aaaacttggc cagttgattc gagtttggtg attttagttt
    24121 gtaggacagt agggaattgt tcatcgactc cgaggactga agccgcatgt tctggagttg
    24181 gaaagactat ttcgttgatt tcttcaaagt gttcatcatt catgtgaatg tggtagtcta
    24241 acttgaaacg attatagata gaactatagt attcaaggtt tggataattt gcgttgatat
    24301 attgttctgg gatgtaggat gtatggtaga tataaacgac accgtttgat tcgcggatac
    24361 gttcaatctt gtagtagaat tgatcgccgc gtagacccaa tttttccaag taaacaagct
    24421 tgtttccgcg ttcaattgaa agaacagtta ccttatcatc tttagcattg aagagttcaa
    24481 tatctgaaaa ctctacaagc ttgtgtttgc gtgcacgtga aacgaaggtt ccttttcctt
    24541 gttggcggac aatatagcca tctttggcaa ggtcgtttaa ggcgcgaaca actgtgatag
    24601 agctgacatc gtacattgaa atgagttctg cttcagtgta aaatttatct ccactgctaa
    24661 actgcccaga gatgatttta ttttttaatt cgtcttttat gtattgatat ttgggaattg
    24721 ccatattttt acctctattt tccttctcca taggaaattt taccataaaa gcgaaaaaaa
    24781 tagtagtctt ttgaataaaa aattagaata atagtttgaa aaattaagcg atatagggat
    24841 tgctcatatt ttatcaatgt attctataca aaattatttt aggtcccata ttccgaattt
    24901 aaagagcgtt tgaaatgggt ctaaaaatgt ttgaaactaa ttgaatgtgt gtataatagt
    24961 aaaaatgcag taaatatccc gacaatttct attgacaatt caaacagatt ggtttataat
    25021 taatataaca acaaatgaaa gcgcaaactt tcgcggtcgg aaggtagttt tatgacacga
    25081 tttgagatac gagatgattt ctatctcgat ggaaaatcat ttaagatttt atctggtgcc
    25141 attcattatt ttagggttcc tccagaggat tggtatcatt cgctctataa cttgaaggct
    25201 cttggtttta atacggtaga gacttatgtt gcttggaatt tacacgagcc tcgtgaaggt
    25261 gagtttcatt ttgaaggtga tctggattta gagaaatttc tccaaatagc gcaggatttg
    25321 ggtctctacg caattgtgcg tccgtctcca tttatctgtg cggaatggga attcggtggc
    25381 ttaccagctt ggctcttgac caagaacatg cgaattcgct catccgaccc agtatatatc
    25441 gaggcagttg gtcgctacta tgatcagtta ttgccaagac tggtgcctcg tttgttggac
    25501 aatggtggca atattctcat gatgcaggtt gaaaatgagt atggttctta cggagaagat
    25561 aaggcttacc tgagagcgat tcgacagcta atggaagagt gtggcgtaac ctgtcccctc
    25621 tttacatcag atggtccatg gcgagctact ctgaaagctg gaaccttaat tgaagaggac
    25681 ctctttgtaa caggaaactt tggttctaag gcaccttaca acttttcgca gatgcaggaa
    25741 ttctttgatg aacatggtaa gaaatggcca ctcatgtgta tggagttctg ggatggttgg
    25801 ttcaatcgct ggaaagaacc gattatcaca cgggatccta aggaattggc agatgcagtt
    25861 cgagaggttt tggaacaagg ctctatcaat ctttacatgt tccacggtgg tacaaacttt
    25921 ggtttcatga atggttgctc agctcgagga actttggacc tgccacaagt tacgtcttat
    25981 gattacgatg cccttctgga tgaagaagga aatccaactg ctaaatatct tgcagtcaag
    26041 aagatgatgg caacacattt ttcagagtat ccgcagttgg aaccactcta caaagagagt
    26101 atggagttgg atgctattcc actagttgaa aaagtttctt tgtttgaaac cttagatagc
    26161 ttgtcaagtc ctgtagaaag tctctatcct caaaagatgg aggagctggg acaaagttat
    26221 ggctacctac tttatcgaac agaaacaaac tgggatgcag aagaagaaag acttcgtatc
    26281 attgatggtc gagatagggc ccagctgtat gtcgatggtc agtgggttaa aactcaatat
    26341 cagacagaga ttggggaaga tattttttat caaggtaaaa agaaagggct atctaggtta
    26401 gatatcttga tagaaaatat ggggcgtgtc aactatgggc ataagttctt agcggatacg
    26461 caacgtaagg gaattcggac aggggtctgt aaggatctgc atttcttact aaactggaaa
    26521 cactatccac tcccactaga caatcctgag aaaattgatt tttcaaaagg atggactcaa
    26581 ggacaaccag ccttttacgc ttatgacttt acagtcgaag agccaaaaga tacttaccta
    26641 gacttgtctg agtttggtaa gggagttgcc tttgtcaatg ggcagaatct aggacgtttt
    26701 tggaacgttg gcccaactct ctcactttat atccctcata gctatctcaa ggaaggtgcc
    26761 aaccgtatca ttatctttga aacagaaggt caatataaag aagagattca tttaactcgt
    26821 aaacctacac taaaacatat aaagggggaa aacttatgac aattgtagga tgccgtattg
    26881 atggacgttt gatccacgga caagtagcca atctttgggc tggaaaacta aatgtttcac
    26941 gcattatggt tgtagacgac gaagttgtca acaacgatat tgaaaagagt ggtttgaaac
    27001 ttgcgacacc accaggtgtg aaattgagta ttttgccagt tgagaaagct gcagccaata
    27061 ttcttgctgg taaatacgat agccaacgtc tctttatcgt ggctcgtaaa ccagaccgct
    27121 tccttggttt ggtagaagca ggtgtaccac ttgaaaccct taatgttggg aatatgtctc
    27181 aaacaccaga aactcgttct attacacgtt ctatcaacgt agtagacaag gatgtggaag
    27241 acttccacaa actggcagaa aaaggtgtta aacttactgc tcagatggtt ccaaatgatc
    27301 caatttcaga ctttttgagc ttattaaaat aggaaaaaaa tttttaggag gtcattgtta
    27361 tgatacaatg gtggcaaatt ttacttctca ctttgtactc agcttatcaa atctgtgatg
    27421 agttgacgat cgtttcatct gcaggttccc ctgtatttgc tggtttcatt actggtttaa
    27481 tcatgggaga tgtgactact ggtttactta tcggtggtaa cttgcaactg ttcgttcttg
    27541 gggttggtac cttcggtggt gcttctcgta tcgacgcaac ttctggtgcg gttcttgcga
    27601 cagccttctc tgtttcacaa ggaattgatg caccgcttgc aattactaca atagctgtac
    27661 cagtagcagc tctcttgact tacttcgacg ttcttggtcg tatgactact accttcttcg
    27721 ctcaccgtgt ggatgctgca atcgaacgct ttgactataa aggtattgaa cgcaactact
    27781 tgcttggtgc gattccgtgg gctctatctc gtgcccttcc agtcttcttt gcccttgctt
    27841 ttggtggtgc ctttgtacaa tcagtagtag acttcgttga agcctacaaa tgggttgcag
    27901 atggcttgac acttgcagga cgtatgcttc caggtcttgg atttgcaatc ttgcttcgtt
    27961 accttccagt taaacgtaac cttcactacc ttgctatggg atttggtttg acagctatgt
    28021 tgactgttct ttactcatat gtaacaggtc ttggtggcgc tgttgctggt atcgtaggta
    28081 ctcttcctgc tgaagttgct gaaaaaattg gtttcgtgaa caacttcaaa ggtttgtcta
    28141 tgattggtat ttctatcgta ggtattttcc ttgcagtgct tcacttcaaa aatagccaaa
    28201 aagtagctgt agcagcacct tctacaccat cagaaagtgg ggaaatcgaa gatgacgaat
    28261 tctaattaca aacttacaaa agaagatttt aatcaaatca acaaacgtag cttgtttact
    28321 ttccaattag gttggaacta cgaacgtatg caagcttctg gttaccttta catgatcttg
    28381 cctcagttgc gtaaaatgta tggtgatgga actcctgaat tgaaagaaat gatgaaagtt
    28441 catactcaat tcttcaatac ttcaccattc ttccatacca ttatcgctgg ttttgacctt
    28501 gccatggaag aaaaagatgg tgtaggttca aaagacgctg ttaacggtat caagacaggt
    28561 ttgatgggac cattcgctcc tcttggggat acaatctttg gttcacttgt acctgctatc
    28621 atggggtcag tcgcagcaac tatggctatc gctggccaac cttgggggat cttcctttgg
    28681 attgcagttg cagtagcgta tgacatcttc cgttggaaac agttggaatt tgcttacaaa
    28741 gaaggggtta accttatcaa caacatgcaa agtaccttga cagctttgat tgacgctgca
    28801 tctgtacttg gtgtcttcat gatgggtgct cttgtagcaa cagtgattaa ctttgaaatt
    28861 tcttacaagt tgccaatcgg tgaaaagatg attgatttcc aagacatctt gaaccaaatc
    28921 ttcccacgtt tgcttccagc aatctttact gcctttatct tctggttgct tggtaaaaaa
    28981 ggtatgaact ctactaaagc tatcggtatt attatcgtac ttgctttggc tctttctgcc
    29041 cttggtcact ttgcacttgg aatgtaattc cttatgacta aatcattaat tttggtgagc
    29101 catggtcgct tctgtgagga gcttagaggt agcacagaaa tgattatggg cccacaagac
    29161 aacatttaca cagtagctct tcttccagaa gatggcccag aagaatttac tgctaaattt
    29221 gaagctgtta ttgaaggatt ggatgatttc ctagtctttg cggatcttct cggtgggaca
    29281 ccttgtaatg tggtgagtcg cttgatcatg gaaggtcgtg atattgacct ttacgcaggg
    29341 atgaatcttc caatggtgat tgaatttatc aatgcgagcc ttacaggcgc agatgcggac
    29401 tacaagagcc gtgctgcaga aagcattgtg aaagttaatg acctgttagc gggcttcgat
    29461 gatgacgaag atgaataata ctcttcgaaa atctcttcaa actacgtcaa cgtc
//
LOCUS       DAVBCW010000015        28858 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVBCW010000015 DAVBCW010000000
VERSION     DAVBCW010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28858
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.015"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            <1..825
                     /gene="leuB"
                     /locus_tag="VQ514_000564"
     CDS             <1..825
                     /gene="leuB"
                     /locus_tag="VQ514_000564"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HET2499366.1"
                     /translation="AAIGSPQYDGAAVRPEQGLMALRKELNLYANIRPVKIFDSLKYL
                     SPLKPERISGVDFVVVRELTGEIYFGDHILEERKARDINDYSYEEVERIIRKAFEIAR
                     NRRKIVTSIDKQNVLATSKLWRKVAEEVAQDFPDVTLEHQLVDSAAMLMISNPAKFDV
                     IVTENLFGDILSDESSVLSGTLGVMPSASHSENGPSLYEPIYGSAPDIAGQGIANPTS
                     MILSVAMMLRDSFGRYEDEERIKRAVETSLAAGILTRDIGGQASTKEMTEAIIARL"
     gene            822..1091
                     /locus_tag="VQ514_000565"
     CDS             822..1091
                     /locus_tag="VQ514_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HET2499367.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKFKARRRTWRIQEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            1095..1454
                     /locus_tag="VQ514_000566"
     CDS             1095..1454
                     /locus_tag="VQ514_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HET2499368.1"
                     /translation="MAGSSREHAAWALANYGFKVVIAGTFGDIHYNNELNNGLLPIVQ
                     PREVREKLAQLKPTDHVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            1616..2184
                     /locus_tag="VQ514_000567"
                     /pseudo
     CDS             1616..2184
                     /locus_tag="VQ514_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007122522.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
     gene            2278..2343
                     /locus_tag="VQ514_000568"
                     /pseudo
     CDS             2278..2343
                     /locus_tag="VQ514_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849983.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
     gene            2506..4437
                     /locus_tag="VQ514_000569"
     CDS             2506..4437
                     /locus_tag="VQ514_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HET2499369.1"
                     /translation="MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEW
                     PLDRILTMSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIYWNKKTNK
                     YKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTK
                     LLCIYTEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELMSKTYPELEPSKL
                     GTMLFEYSKLFMENKEDNSTMDSSNNFRHQLTQSLLKSPNLILRGAPGTGKTYLAKEI
                     AKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKE
                     TQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSGVPGWSLP
                     SKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKFVFIIDEI
                     NRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIGTMNDIDR
                     SVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIENVQELNSH
                     YHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFELTNNEQ
                     KDQAVADDNESDENDDADY"
     gene            4424..5776
                     /locus_tag="VQ514_000570"
     CDS             4424..5776
                     /locus_tag="VQ514_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HET2499370.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKKQKILETINQEIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLHINLTSLDVALSREERLYQLLMYLFPKYLQAAIRKGLYKEYHRFSHNDSHVKGV
                     IDVRNHLKKNLPFTGNIAYTTREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKIIRGNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLVFPSKDKVIDNEMGNL
                     AGYGALLKKWSIQIPQKSSSYSAFCKMMENSDYSAFCKMMENSEENFKAIIDEEVGRK
                     "
     gene            5951..6937
                     /locus_tag="VQ514_000571"
     CDS             5951..6937
                     /locus_tag="VQ514_000571"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HET2499371.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKQMHDQGLIASISVGVKDYEY
                     DFVSQLKTDAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            7001..7072
                     /locus_tag="VQ514_000572"
     tRNA            7001..7072
                     /locus_tag="VQ514_000572"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7034..7036,aa:Arg,seq:ccg)
     gene            7255..7953
                     /locus_tag="VQ514_000573"
     CDS             7255..7953
                     /locus_tag="VQ514_000573"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HET2499372.1"
                     /translation="MKELQTVLKNHFAIEFADKNLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            7944..11483
                     /gene="smc"
                     /locus_tag="VQ514_000574"
     CDS             7944..11483
                     /gene="smc"
                     /locus_tag="VQ514_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HET2499373.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKSEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAALEDKRNS
                     LSKEKSDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLAIAKEKASQQKEELETAKEQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDNLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDMHYQTALEIALGASSQHIIVEDEESATKAIDFLKR
                     NRAGRATFLPLTTIKARTISSQNQDAITVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDAVEHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAADEASLRSEEAALKTLQDQMATLTERLEVIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEEIDRLSEGDWQADKEKCQDRLAAIASDKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVADIERLGKELDNLDFEQEEIQRLLQEKVDN
                     LEKVDTELLSQQAEEAKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRYLQSQLTDQYQISYTEALEKAHELENLNLAEQEVQDLEK
                     AIRSLGPVNIEAIDQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKDRFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            11480..12274
                     /locus_tag="VQ514_000575"
     CDS             11480..12274
                     /locus_tag="VQ514_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2499374.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFAGVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYI
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNDNVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPAAPENARPEILELAKTVIGHHKDQSVIAYMEGL"
     gene            12274..13092
                     /locus_tag="VQ514_000576"
     CDS             12274..13092
                     /locus_tag="VQ514_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2499375.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKATLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDDHEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFADLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAVANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            13096..14373
                     /gene="ftsY"
                     /locus_tag="VQ514_000577"
     CDS             13096..14373
                     /gene="ftsY"
                     /locus_tag="VQ514_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HET2499376.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVS
                     QEEAEVEIVEQAVFQEEEIQDTVEESLDLEPVVEVSQKEVEEFPHSEEGNTEFLATVE
                     ENNSEILEAERPQTEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEELEEL
                     LIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYEKDGSYDESIHFQ
                     DNLTVMLFVGVNGVGKTTSIGKLAHRYKRAGKKVMLVAADTFRAGAVAQLAEWGRRVD
                     VPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGRIIKR
                     VVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAIREEL
                     NIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(14419..15906)
                     /locus_tag="VQ514_000578"
     CDS             complement(14419..15906)
                     /locus_tag="VQ514_000578"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET2499377.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYQSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATANKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRGVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVCASWKLIDRIEKLWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDITYRQDGRLE"
     gene            complement(16055..16795)
                     /locus_tag="VQ514_000579"
     CDS             complement(16055..16795)
                     /locus_tag="VQ514_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2499378.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            complement(16795..18960)
                     /locus_tag="VQ514_000580"
     CDS             complement(16795..18960)
                     /locus_tag="VQ514_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HET2499379.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALAEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            19133..20086
                     /locus_tag="VQ514_000581"
     CDS             19133..20086
                     /locus_tag="VQ514_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2499380.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAYTLYGIS
                     LLALIGLARNFGEAGQNLASYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            20163..22151
                     /gene="uvrB"
                     /locus_tag="VQ514_000582"
     CDS             20163..22151
                     /gene="uvrB"
                     /locus_tag="VQ514_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HET2499381.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARIEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMTYNEEHDIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKVLD"
     gene            22161..22724
                     /locus_tag="VQ514_000583"
     CDS             22161..22724
                     /locus_tag="VQ514_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2499382.1"
                     /translation="MIYLRKLKREDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKQTRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            22690..23178
                     /locus_tag="VQ514_000584"
     CDS             22690..23178
                     /locus_tag="VQ514_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2499383.1"
                     /translation="MTVIIKSMETPEEIEGKSLVHWQTWREAYDDLLPAEFQETMTLE
                     RCRFFSQKYPENTLIAIGDLKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LMKAALTALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIKEKRMVFY
                     SK"
     gene            23252..23719
                     /locus_tag="VQ514_000585"
     CDS             23252..23719
                     /locus_tag="VQ514_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HET2499384.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRAPENNRWSGYAFPGGH
                     VENDEAFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVICYKATEFSGTLQS
                     SEEGEVSWVQKDQIPNLNLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF"
     gene            complement(23716..24231)
                     /locus_tag="VQ514_000586"
     CDS             complement(23716..24231)
                     /locus_tag="VQ514_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HET2499385.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(24243..24770)
                     /locus_tag="VQ514_000587"
     CDS             complement(24243..24770)
                     /locus_tag="VQ514_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2499386.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            complement(25048..25611)
                     /locus_tag="VQ514_000588"
     CDS             complement(25048..25611)
                     /locus_tag="VQ514_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2499387.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            complement(25595..26146)
                     /gene="coaC"
                     /locus_tag="VQ514_000589"
     CDS             complement(25595..26146)
                     /gene="coaC"
                     /locus_tag="VQ514_000589"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HET2499388.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            complement(26158..26847)
                     /gene="coaB"
                     /locus_tag="VQ514_000590"
     CDS             complement(26158..26847)
                     /gene="coaB"
                     /locus_tag="VQ514_000590"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HET2499389.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISADQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            27077..28747
                     /locus_tag="VQ514_000591"
     CDS             27077..28747
                     /locus_tag="VQ514_000591"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HET2499390.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELEIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNALSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
BASE COUNT         8922 a         5128 c         6403 g         8405 t
ORIGIN      
        1 gcagctatcg gtagtcctca gtatgatgga gcagcggttc gccctgaaca aggcctgatg
       61 gctctccgta aggaactcaa tctttacgct aatattcgtc ctgtaaaaat ctttgacagt
      121 ctcaagtatt tgtcaccact taaaccggaa cgaatttctg gtgtagactt tgtcgtggtg
      181 cgtgaattga caggcgagat ttactttgga gatcatattc ttgaagagcg caaagcgcgt
      241 gatatcaacg actatagcta tgaggaagtg gagcggatta ttcgcaaagc ctttgaaatt
      301 gcaagaaatc gcagaaaaat cgttactagt atcgataagc aaaatgttct agcgacctca
      361 aaactctggc ggaaagtagc tgaggaagtc gcacaggatt tcccagatgt aaccttggaa
      421 caccagctgg tagactcagc tgctatgctt atgattagca atcctgctaa gtttgatgtt
      481 attgtaacgg agaatctttt tggagatatt ttatctgatg aatcaagcgt cttatctggt
      541 acacttgggg ttatgccatc agccagtcat tctgaaaatg gaccaagtct ctatgaacct
      601 atttacggtt cagcacctga tattgcaggt caaggaattg ccaatcctac ttccatgatt
      661 ttatcagttg ccatgatgtt gagagatagt ttcggacgtt atgaggatga agagcgtatc
      721 aaacgtgctg ttgagacaag tctggcggca ggaattttaa cgagagatat aggggggcag
      781 gcttcaacaa aggaaatgac ggaagctatt attgcaaggt tatgaagtta gacgaaaaaa
      841 ttactctagt ccttttgatt tggaatgtca tcattttctt gatttatggc attgacaaat
      901 tcaaggcaag aagaagaact tggcgcatcc aagagaaaat cttactcatt ttagccctta
      961 cttgtggtgg ttttggggcc tggttagctg gaatcacttt tcaccacaag actcgaaaat
     1021 ggtactttaa aacagtttgg tttcttggga tggtgaccac actagtagcc ttatatttta
     1081 tttggaggta atggatggca gggtcttcga gggaacacgc tgcttgggct ctagcgaact
     1141 atggttttaa ggtcgtgatt gcaggaactt tcggtgacat tcattacaat aatgaactca
     1201 ataatggcct gttgccaatc gttcagccta gagaggttag agagaaacta gcccagctaa
     1261 aaccaaccga ccatgtaact gtggacttgg aacaacaaaa aatcatctca ccagttgaag
     1321 aattcacctt cgagatagat agcgagtgga aacataaact cctaaatagt ttggatgata
     1381 tcggtattac cttgcagtat gaagagttga ttgctgctta tgaaaaacaa cgaccagcct
     1441 actggcagga ttagaaaaaa tagaaaagga aatatagtaa actgaaataa gatgtaaaca
     1501 aatgaattag agcttaacat ccatttccag caatttttta gaaactacag tggactattc
     1561 tggattcaac acattataaa attatgacaa gacacattca caagaaggct acgacatttt
     1621 aaaaggtgag ggcggatgta tcgtttgccc tactaaagtt ggttacatta tcatgaccag
     1681 tgacaaggca ggacttgagc gtaagttcgc agccaaagaa cgtaagcgta acaaaccagg
     1741 tgttgttctc tgcggtagca ttgatgaact ttgcgcttta gcgcaactca acccagaaat
     1801 tgaagcattc tactaaaaac attgggatga agatattctt cttggttgta tccttccttg
     1861 gaaaccagaa gcctttgaaa aactcaaagc atacggggat ggccgtgaag aacttatgac
     1921 tgatgtacgt ggtactagct gttttgttat caagtttgga aaagcaggtg aacaattggc
     1981 tgccaagctt tgggaagaag gtaaaatggt ctacgcctca tctgcttcaa tgacaaaacg
     2041 attgaaactc gctatgagca aggtgtaatg gtgtctatgg tcgataagga cggcaaactc
     2101 atcccagaac aaggaggagc acgttcaact tcaccagctc cagttgtgat ccgtaaaggg
     2161 cttgacattg atatagtaaa atgaagtaag agcaggacaa attaatcagg acagtcaaat
     2221 cgatttctaa caatgtttta gaagtagagg tgtactattc tagtttcaat ccactataaa
     2281 atcatgatgc acctgtcaga tacttttaac tcatgggact accgtcaggt tgagtattat
     2341 taggatagag aagaagtcta gtgttaagga cattacagct cataatacta ggctttttta
     2401 tagaaattct tttctttttc tataggatat ggtattctat gtagaaaata tatgttaata
     2461 agtaatgcca atatttaaac atcattagta aaaggagtta gattgatgaa taaaagaaaa
     2521 gttagtttag aagattttta taaatggtat agtctaaata aagaagagtt attaaataag
     2581 gcaactgttg gtgaaaagtt taatgataaa ttaaaagaag agtttctcca ggaatggcct
     2641 ttggatagga ttttaacaat gtcaatcgat gaatatgtaa taggaaaggg acagcaaaat
     2701 aagtctttat gctacgctct tgagaaggga aaatacaaaa atctatttct tggaatttct
     2761 ggtggctcag cttcaaaatt tggtatttat tggaataaaa aaacaaacaa atataaagat
     2821 caagctaata atgagatttc agagttggat cagcgatttt caaaattaaa atcagatttg
     2881 tatgaaatta tcaaagaagg tattcgtttt aactttgaaa atcctatttt tgatatgaaa
     2941 agatcaacaa atgaatttat tggtcgttct gctatggtga caaaattact ttgtatctat
     3001 actgagggag atcctttctt tggtgtaaat attaatagtc agaaagaatt ttggaaccac
     3061 tttgtttctc agacaaatca aggtggacct tatctgcaaa atcataaaat aattgaactg
     3121 atgtccaaaa cttatcctga gttggagcca tcgaaattag gaactatgct ttttgagtat
     3181 tctaagcttt ttatggaaaa taaggaagac aatagtacaa tggattcatc aaacaatttt
     3241 cgtcatcaat taactcaatc tctattaaag tctccaaacc ttatccttcg tggtgctcct
     3301 ggcacaggaa agacttatct tgctaaagaa attgccaaag aattaacgga tggcaacgaa
     3361 gatcaaatcg gatttgtaca atttcaccca tcatatgatt atacggattt tgtagaaggt
     3421 ttaagaccag tatcaaatgg ggatggagct attgagttta ggctacagga cggtattttt
     3481 aaagattttt gtcagaaagc aaaagaaacc caattgattg gaggacaaga taattttgat
     3541 gaggcttggg attcttactt agaatatata aatgttgctg aagaaaaaga atatataaca
     3601 aaaacatctt acttatctgt taatagtaga caaaatttgt cagtaaatta tgatagtggt
     3661 gttccaggat ggtcactacc tagcaaatat gtttacgagt tgtataaaga taaaaattat
     3721 aataagcaag aatactacaa aagtggtgga aaaactgtcc tagaaacatt gagaaagaga
     3781 tttggtttga aagactatgt ttccccaaca gaaattgata ctgataagaa attcgttttc
     3841 atcatcgatg aaatcaatcg tggggagatt tctaagattt ttggcgaact ctttttctct
     3901 atcgaccctg gctatcgtgg tgaaaaagga agtgtttcta cccaatatgc aaatctacac
     3961 gaaactgatg aaaagttcta tatccccgaa aatgtttaca tcatcggaac tatgaatgat
     4021 attgatcgtt cagtggatac ctttgatttt gctatgcgtc gtcgttttcg ttttgttgaa
     4081 gttactgccg agggtcaagt tggcatgttg gataaagagc tgaatatcca tgcagaagaa
     4141 gcaaaaattc gtctaagaaa cttgaacgct gctatcgaaa atgttcagga attaaacagt
     4201 cattatcata ttggaccaag ttattttctt aagttgaagg atgtagattt tgactatgaa
     4261 ttactctggt ccgattatat taagcctctc ctagaagact acttgcgagg ttcttatgat
     4321 gaggttgaaa ctttggaaac tttgaaaaaa gcatttgaac tgacaaataa tgagcaaaaa
     4381 gatcaggcag tagctgatga caatgaaagc gatgaaaacg atgatgcgga ttactgataa
     4441 tcaacacaag attattaaag aaaaatttgt tgaagaatat cctaaactaa gcaatcttct
     4501 tttagacaga accttggaaa gtctatccca agatgaacgt attttcattt ttccaaatga
     4561 tttgactcat actccagatt tggataaaaa acaaaagatt ttggaaacga tcaatcagga
     4621 aatcaagaca ggaaatgtga ttggttttct tggatatggt caggaaagat taacgatttc
     4681 ctcacgattt tctgatgaga gtaatgacca ttttttgcat tatctcttaa acaaggttct
     4741 tcatatcaat ctcactagtt tagatgttgc tttgtctcgt gaagagaggc tttatcaact
     4801 tttgatgtat ctcttcccca agtatctaca agctgctatt cgaaaaggtc tttataagga
     4861 atatcatcga ttttctcata acgacagtca tgttaaggga gtgattgatg taagaaacca
     4921 tctcaagaaa aatcttcctt tcacgggaaa tattgcctac acaacgagag agttcaccta
     4981 tgataatccc ctcatgcagt tggtccgtca cactattgaa tacattaaga atcagaaaag
     5041 cattggtcaa ggggtactag ataatctctc aactagtcgt gaaaacgtat ctgaaatcgt
     5101 gcgtgtaacg ccctcttata aactagctga tcgtgctaag attattcggg gaaatcaatc
     5161 taaacctata cgtcatgcat actttcacga gtacagaaac ttacaagaac tttgtctgat
     5221 gatcctaaac caagaaaagc acggtttagg gtatcaagat caaaaaatct atggtattct
     5281 ctttgatgtt gcctggcttt gggaagagta tgtttacacc ttgttgccaa aaggttttgt
     5341 acatcccaga aataaggata agacggatgg aatttcagta ttttctgttg ggaaacgaaa
     5401 agtatatcca gatttttatg acagagaacg aaagattgtt ctagatgcaa aatataaaaa
     5461 actggaattg actgaaaaag gaatcaatcg tgaggactta ttccagctga tttcctattc
     5521 ttatatttta aaagctgaga aggctggact tgtttttccg agcaaggaca aggttattga
     5581 taatgagatg ggaaacctag caggttatgg agctttgttg aagaaatggt ctatccaaat
     5641 tcctcagaag tcttcatctt atagtgcatt ttgtaaaatg atggaaaatt cagattatag
     5701 tgcattttgt aaaatgatgg aaaattcaga agaaaatttt aaagcgatta ttgatgaaga
     5761 agtggggaga aaataatttc ccctctttct tttattttta acacaagtaa aacccgaaca
     5821 atatatttca attcataaaa taatttgaca aaaactgtac tttggtttat aataaattaa
     5881 ggaaacgtcg gaaaagacgt agtgatgcga gagcataggt aggtcattac aaaagaaacg
     5941 agacatcgat atgttaaatg aatttccaat ttttgattac gaagatattc aattgattcc
     6001 aaataaatgt gtcattaaaa gccgtgcaga agcggataca agtgtcactt taggaaatca
     6061 cacctttaaa ctacctgttg tgccagcgaa tatgcagacg attttggatg aaaatgtagc
     6121 agagcaactg gctaaaggtg gttacttcta cattatgcac cgttttgatg aggcaggacg
     6181 cattcctttt attaaacaaa tgcacgatca agggctcatt gcttccatct ctgtcggtgt
     6241 taaggactat gagtatgatt ttgttagcca gctcaagact gatgctcctg agtatatcac
     6301 tattgatatt gctcatggtc atgcggatag cgtgatttct atgattcaac atatcaagaa
     6361 agaattgcca gatacctttg tcattgctgg aaatgtggga acaccagaag ctgtgcgtga
     6421 attggaaaat gctggtgcgg atgctactaa ggtcggaatc ggtcccggta aggtttgtat
     6481 caccaaggtt aagactggtt ttggtacagg tggttggcag ttggctgctc tacgctggtg
     6541 tgccaaggct gcgcgtaaac cgattatcgc tgatggagga attcgtactc acggtgatat
     6601 tgctaagtct atccgcttcg gtgctagcat gatcatgatt ggttccctct ttgcaggaca
     6661 cattgaaagt ccagggaaaa cgattgaagt cgatggtgaa cagttcaaag aatattatgg
     6721 ttcagcctca caatatcaaa aaggtgctta caaaaatgtg gaaggcaaac gtatcttgct
     6781 tcctgctaaa ggtcatctgc aagacacttt aactgagatg gaacaagacc ttcaaagtgc
     6841 tatttcttat gcaggtggac gtcaggttgc tgaccttaaa cacgttgatt atgtgatcgt
     6901 gaaaaactcc atctggaatg gggatgcttc ccactaagac ggggtctttg accaaaaaac
     6961 ttgttattag agcaaatttc tgttataata aaacaagttt ccacccttag tgtaatggat
     7021 atcacgtaag attccggttc ttgagatggg ggttcgattc cctcagggtg gatgtaaaca
     7081 tcctaaaaag cctttaatag ggcttttttc tttatcctga cccaaatttg ccgctaaaac
     7141 tgagaatctc tgtctaaaga attcccaaat gctttccagt cccgttttag agtgactcag
     7201 ggggcttttt ttgatataat aaaaaggact gttatcagtt agaaagaggt tggtatgaaa
     7261 gaattacaaa ctgtactaaa gaatcatttt gcaatcgaat ttgcagacaa aaatttactg
     7321 gaaactgcct ttactcatac gagttatgcc aatgagcacc gcctcttaaa aatttcacac
     7381 aatgaacgct tggaattttt aggagacgct gttctacagt tattgatttc agaatatcta
     7441 tataaaaaat atcctaaaaa gcctgaaggt gacctatcaa aactccgtgc tatgattgtc
     7501 cgtgaggaga gtttagctgg ttttgcgcgt gattgccagt ttgaccagtt tatcaagttg
     7561 ggtaaagggg aagaaaaatc aggtggtcgc aatcgtgaca ccattcttgg tgatgccttt
     7621 gaagcctttc ttggtgccct tcttttggat aaggatgtgg ccaaggtcaa ggaatttatc
     7681 tatcaagtca tgattcctaa ggttgaagca ggcgaatttg agatgattac agactataaa
     7741 acccatctcc aagagttact tcaggtcaat ggtgatgtgg ctattcgtta tcaggtgatt
     7801 tctgaaacag ggcctgctca cgataaggtc tttgatgtag aagttcttgt tgaaggtaag
     7861 agcatcggtc aaggccaagg tcgttctaag aaattagcag agcaggaagc tgccaaaaat
     7921 gccgttgaga aggggctgga ttcatgtatt taaaggaaat cgaaattcag gggttcaagt
     7981 cttttgctga taagaccaag gtcgtttttg accaaggtgt gacggcagtt gttggaccca
     8041 atggatctgg aaagtccaat attacagaaa gtctgcgttg ggctttgggg gagtctagtg
     8101 tcaagagtct ccgtgggggc aagatgccgg atgtcatctt tgctggaaca gaaagtcgca
     8161 aaccgctcaa ttatgcttct gtagttgtga ctctggataa tcatgacgga tttatcaagg
     8221 atgcaggtca agaaatcagg gtggaacgcc atatctatcg tagtggagat agcgaataca
     8281 agattgacgg caagaaagtc cgtctgcgtg atattcatga ccttttcttg gatacaggtt
     8341 tgggacgaga ttccttctct attatttccc aagggaaggt tgaggagatt tttaattcca
     8401 agtctgagga acgacgagct atttttgaag aagctgctgg agttttaaaa tacaagactc
     8461 gcagaaaaga aaccgagagt aaactgcagc aaactcagga taatctggac cgcttagagg
     8521 acattatcta cgagttggat aatcaaatca agcctcttga gaagcaagct gagaatgccc
     8581 gtaaatttct agacttagaa ggtcaacgca aggctattta cttggatgta ttggttgctc
     8641 aaatcaagga aaataaggca gaactagagt cgacagaaga agagttggct caggttcaag
     8701 aactcttgat gagttattac caaaagcgtg aaaaattaga agaagaaaat caaactctta
     8761 aaaagcaacg ccaagattta caggctgaaa tggccaaaga ccaaggcagt ttgatggact
     8821 tgactagtct gattagtgat ttagaaagaa aattagccct atcgaaactg gagtctgagc
     8881 aagtggcctt gaatcaacag gaggcacaag ctcgtttggc tgctttggag gataagagaa
     8941 attcactcag caaagaaaag tctgataaag aaagctcttt agctctgtta gagggaaatc
     9001 tagtccaaaa taatcaaaaa ctcaatcgtt tagaagctga attgctggct ttctcagacg
     9061 atcctgatca gatgattgag ctcttacgtg aacgctttgt agctctttta caagaagaag
     9121 cggatgtctc aaaccagttg acccgtattg agaatgagtt ggaaaatagt cgtcagcttt
     9181 ctcaaaaaca agcagatcaa ctagaaaagc tgaaagagca attagctata gctaaagaga
     9241 aggctagtca gcaaaaggaa gagcttgaaa ctgccaagga gcaggttcag aaattattgg
     9301 ctgactacca agctattgcc aaggagcaag aggagcagaa aacttcctat caagctcaac
     9361 aaagccaact ctttgaccgt ctggataatc tcaaaaacaa gcaggccaga gctcaaagtt
     9421 tggaaaatat cctgagaaat catagtaact tttatgcagg tgttaagagt gttctccaag
     9481 aaaaagatcg cctaggtggg attattggtg cagtcagtga gcatctgacc tttgatatgc
     9541 attatcaaac tgccctagaa attgcgcttg gagcgagtag ccaacatatc atcgtagaag
     9601 atgaagagtc ggcaaccaag gctattgact tccttaaacg gaacagagcc ggtcgtgcaa
     9661 cctttcttcc tttgaccact atcaaggcgc gtacgatttc tagtcagaac caagatgcta
     9721 tcactgtaag cccaggtttc cttgggatgg cagatgagtt ggtgactttt gatactagac
     9781 tggaagccat tttcaagaac ttgctagcta cgacggctat ctttgatgcc gtagaacatg
     9841 cgcgtgcagc agctcgccaa gttcgttatc aggttcgtat ggtgacattg gatgggacag
     9901 aattacgcac gggtggttcc tatgcgggtg gtgccaatcg ccaaaataac agtattttca
     9961 tcaagccaga actggagcaa ttacaaaaag aaattgctgc agatgaagca agcttgcgtt
    10021 cagaagaagc ggctttgaag accttacaag accagatggc tacattgaca gaaagattag
    10081 aagtcatcaa atctcaagga gaacaggcac gtattcagga gcaaggcttg tccctcgctt
    10141 atcagcaaac tagtcagcaa gttgaagaac tggaaactct ttggaaactc caagaagagg
    10201 aaatagatcg tctttccgag ggagattggc aagcggataa ggaaaaatgc caagatcgtc
    10261 ttgctgcaat cgccagtgac aagcaaaatc tggaagctga gattgaagag attaagtcta
    10321 acaaaaatgc catccaagaa cgctatcaaa acttgcagga agagctagcg caagctcgtt
    10381 tgcttaagac agaactgcaa gggcaaaaac gttatgaagt tgctgatatt gaacgcttag
    10441 gcaaggaatt ggacaatctt gattttgaac aagaggaaat ccagcgcctt cttcaagaaa
    10501 aggttgataa tcttgagaag gttgatacag aattgctcag tcaacaggcg gaagaagcca
    10561 aaactcagaa aacaaatctc caacaaggtt tgattcgcaa gcagtttgag ttggatgata
    10621 ttgaaggtca actggatgat attgctagtc atttggatca ggctcgccaa cagaatgaag
    10681 agtggattcg caagcaaaca cgtgctgaag ccaagaaaga aaaggtcagc gagcgcttgc
    10741 gctatttaca aagtcaatta acagaccaat atcagattag ctatactgaa gctctagaaa
    10801 aggcgcatga attggaaaac ctcaatctgg cagagcaaga ggtgcaggat ttagagaaag
    10861 ctattcgctc actggggcct gtcaatatag aagctattga ccagtacgaa gaagttcaca
    10921 atcgtctgga tttcctaaat agccaacgag atgatatttt gtcagcgaaa aatctgctcc
    10981 ttgagaccat tacagagatg aatgatgagg tcaaagatcg ctttaaatca acctttgagg
    11041 ccattcgtga atcctttaaa gtgaccttca aacagatgtt tggaggaggt caggcagact
    11101 taatcttgac tgagggagac ttgttgacag ctggggttga gatttctgtt caacctccag
    11161 gtaagaaaat ccagtcgctt aacctcatga gtggtggtga aaaagcctta tcagctcttg
    11221 ctttgctttt ttctatcatt cgagttaaga ctatcccatt tgtcatcttg gatgaggtag
    11281 aggccgcgct ggacgaagcc aatgttaaac gttttgggga ttacctcaat cgatttgaca
    11341 aggacagcca gtttatcgtc gtaacccacc gtaagggaac catggcagcg gctgattcta
    11401 tctatggagt gaccatgcaa gaatcggggg tctcaaaaat tgtttcggtt aagttaaaag
    11461 atttagaaag tattgaagga tgacaattaa actagtagca acggatatgg acggaacctt
    11521 cctagatggg aatggacgct ttgatatgga tcgtctcaag tctctcttgg cttcctacaa
    11581 ggaaaaaggg atttactttg cggtggcttc gggtcgggga tttctgtctc tggaaaaatt
    11641 atttgctggt gttcgtgatg acattatttt catcgcggaa aatggcagtt tggtagagta
    11701 tcaaggtcag gacttgtatg aagcgactat gtctcgtgac ttttatctgg caacttttga
    11761 aaagctgaaa acgtcacctt atatagatat caataaactg ctcttgacgg gtaagaaggg
    11821 ttcatatgtt ctagatacgg ttgatgagac ctatttgaaa gtgagtcagc attataatga
    11881 aaatatccaa aaagtagcga gtttggaaga tatcacagat gacattttca aatttacaac
    11941 caacttcaca gaagaaacgc tagaagctgg tgaagcttgg gtcaatgata atgtccctgg
    12001 tgtcaaggct atgacaactg gctttgaatc tattgatatt gttctggact atgtcgataa
    12061 gggtgtagct attgttgaat tagctaaaaa acttggcatc acaatggatc aggtcatggc
    12121 ttttggagac aatcttaatg acttacatat gatgcaggtt gtgggacatc ctgcagctcc
    12181 tgaaaatgca cgaccagaga ttttagaatt agcaaagact gtgattggtc accataagga
    12241 ccagtcggtt atagcttata tggagggctt ataatggcag atataaaatt aatcgcattg
    12301 gacttggacg gtaccttgct gactactgat aaaaggctga cggatcgcac caaggcaact
    12361 ttgaaagctg cgcgtgatcg tggtatcaag gtcgtattga caactggtcg tcccttaaaa
    12421 gccatggact tctttctcca tgagttaggg actgacgacc acgaagatga gtataccatt
    12481 acttttaatg gtggattagt tcagaaaaat acaggagaaa tccttgataa aacagtcttt
    12541 tcatatgatg atgtggcacg tttgtatgaa gaaacagaga aattatcact gcctcttgac
    12601 gctatctcag aaggaactgt ttatcaaatt caatcggacc aagaaagtct ttatgccaaa
    12661 ttcaatccag ctttgacctt tgttccagtg gactttgcag atctgtctag tcaaatgacc
    12721 tacaacaaat gcgtgactgc ctttgctcaa gaacccttgg atgcagccat tcagaagatt
    12781 tctccagaat tgtttgacca gtatgaaatc tttaaatcac gtgaaatgtt gctagagtgg
    12841 tcaccaaaga atgttcataa agcaacaggc ttggcaaaac tgatcagcca tcttggaatt
    12901 gatcaaagtc aagtgatggc ctgtggtgat gaagccaatg acctctctat gattgaatgg
    12961 gcagggctcg gtgttgctat gcaaaacgct gttcctgaag tcaaggctgt cgcaaatgta
    13021 gtgacgccaa tgaccaacga tgaggaagct gtcgcctggg ctatcgaaga atacgtgcta
    13081 aaggagaact aagatatggg attgtttgac cgtctattcg gaaaaaaaga agaacctaaa
    13141 atcgaagaag ttgtaaaaga agctctggaa aatcttgatt tgtctgaaga tgttgatcct
    13201 accttcacag aagttgagga agtttctcag gaagaagcag aggttgaaat tgttgaacaa
    13261 gctgtgttcc aagaagagga aatccaagac acagttgaag aaagtctgga tttagagcca
    13321 gttgtagaag tttctcaaaa agaagtcgaa gaatttccac actcagaaga agggaatact
    13381 gagtttctag cgactgtaga agaaaataat tctgaaattc ttgaagcaga aaggcctcaa
    13441 acagaagaaa ctgttcagga aaaatatgac cgcagtctta agaaaactcg cacaggtttc
    13501 ggtgcccgct tgaatgcctt ctttgctaac ttccgctctg ttgacgaaga atttttcgag
    13561 gaactggaag aactgttgat tatgagtgat gttggtgtcc aagtcgcttc taacttaacg
    13621 gaggaactac gttacgaagc caagcttgaa aatgccaaga aacctgatgc acttcgtcgt
    13681 gtcatcattg agaaattggt tgagctttat gaaaaggatg gtagctacga tgaaagcatc
    13741 cacttccaag ataacttgac agttatgctc tttgttggtg tgaatggtgt tgggaaaaca
    13801 acttctatcg gaaaactagc ccaccgctac aaacgagctg gtaagaaggt catgctggtt
    13861 gcagcagata ccttccgtgc gggtgcagta gctcagctag ctgaatgggg ccgacgagta
    13921 gatgttccag tagtaactgg acctgaaaaa gctgatccag ccagcgtggt ctttgatggt
    13981 atggaacgtg ccgtggctga aggtatcgat attctcatga ttgatactgc tggtcgtctg
    14041 caaaataagg ataaccttat ggctgagttg gaaaagattg gtcgtattat caaacgtgtt
    14101 gtgccagaag caccacatga aaccttcttg gcacttgatg catcaacagg tcaaaatgcc
    14161 ctagtacagg ccaaagaatt ttcgaaaatc acacctttaa cgggaattgt tttgactaag
    14221 attgatggaa ctgctcgagg aggtgtggtt ctagccattc gtgaagaact caatattcct
    14281 gtaaaattga ttggttttgg tgaaaaaatc gatgatattg gagagtttaa ctcagaaaac
    14341 tttatgaaag gtctcttgga aggtttaatc taatcagaag caaaaatcct gcaaggcata
    14401 aacttgcagg aaattttttt attctaagcg accatcttga cgataggtga tatctggttg
    14461 ccaagtccat ttggcaccga atttttcaag taggtcaaag ctggcttgag gtcccatgct
    14521 tccagcttta tagtcatgaa gtggggcacc attttcagcc cagagctttt caatacggtc
    14581 aatcaacttc catgacgcac aaacttcatc ccagtggcta aagttagttg agttgttatt
    14641 taggacatca taaatcaatt tttcgtatgg ttctggagaa gcaccagttg cagtcgcatc
    14701 tgtacggtaa tcaagtgagt taggagccaa gttaaattct tctcctactt gcttcccatt
    14761 taggctaaga gagaagcctt ctgttggttg aatatagatg gtcaaaatat ttggagcaag
    14821 tggttctcca aagatagaat ccatttgttt aaagacgatg ttgacatgag ttcctttttc
    14881 agtcagtcgt ttacctgtac ggaaaaagaa aggaacacca cggaatcgat cgctgtctac
    14941 aaagaaggca ccagatgtaa aggtttcagt tgttgattct ggattcacat ttggctcgct
    15001 acgataagag atgtatttca tgccatcaat cttaccagag cggtattgcc cacggataaa
    15061 gtgttctttg agttcttcat cagttggatg atagaggttt ttaaagacct taatcttttc
    15121 agcacgaatc tcgtcttttg tgaagcttgc tggtttgtcc atggcgagga gcgaaagaag
    15181 ttgtagagtg tggttttgga ccatgtcacg gagggcaccg gattggtcat agtagccacc
    15241 acgttcttct acacccaagc gctccgcaaa ggtaatttga acattgtcga taaaatcctt
    15301 gttccaaacg ttttcaaaaa tcaagtttgc aaagcgaact gcaaagatgc tttggatcat
    15361 ttccttacca agataatggt cgatacggaa aatttgttct tcgtcaaatg ttgctaggag
    15421 ttcgtcattc aacttgtttg cagttgcgta atctgtacca aatggttttt caacgatcaa
    15481 gcgctcaaaa cctttgccat cgacaatgtt ttcagatttg aggtgtttgg caatggttcc
    15541 aaagaactga ggtgccatag acaagaagaa gagcttattg tgttcagctt ggtatttttc
    15601 attaagctca gcttgtaatt gacgcaaagc aatataatgt tccgaatcat tgacatcatg
    15661 gctttgatag tagaagtggc tagcaaattc ttgggcttgc tcggtactat ctgccaaatc
    15721 aaggatggac tcgacaacta cagattcaaa atattcctta ctccaaggtc tacgggcagt
    15781 tccaataacg gcaaagtgct tggaaagatt gccggattga tatagtctaa aaagggaagg
    15841 gtagagcttg cgtttagcca ggtctccact cgcaccgaaa attgtaacaa taaccttaga
    15901 tgacatctag ctacctaatt tctattttta ttcctagtct tgctaggtat gaggaaacct
    15961 catttcataa acttaattct aacataattt tccgaaaaat caaaaattcc aatacgaggt
    16021 agaaaaactg caaggaaatc cttacagttt gagtttagac gtttaagacc ttatctaaga
    16081 actctttcag acgagggtgt tgtgggttat caaagatttg gtcaggtgtt ccgtcttcaa
    16141 ggaactcgcc atctgcagta aagataacgc ggttggcaac ctgacgagca aatcccatct
    16201 catgggttac gataatcatg gtcatacctt gctcagccaa ttccttcata acgttaagta
    16261 cgtctccaac catctcaggg tcaagggcag aagttggttc atcgaagagc atgatgtctg
    16321 gattcattgc taggccacga gcgatggcca cacgttgttt ttgaccacct gataggctat
    16381 ctggattggc attagcttta tctgctagtc caaccttttc aagcaactcc attcccaatt
    16441 cctcagcttc ttccttagtc atcaacttgt gctcaatagg agcaaaggtg atgttgtcca
    16501 atacagacat atgagggaag aggttgaagt gttggaatac catgccgata ttttcacgga
    16561 cgtggtcaac attggttgtt ttttcagtta aatcatagcc gttcacagtg atgtgaccgc
    16621 tagtgacttc ttctaaaaga ttgaggctac ggaggaaagt tgacttacca gaacctgaag
    16681 gaccgatgat acaaacaaca tctccttcat agaacttagt cgtaattcct tttaggactt
    16741 cattttttcc atagtgcttg tgtaaatcat ttacatcaat ttttaatttt gccattaacg
    16801 aatcctcttt tctaagcgtt tcgctagtct agtcaaaagt gtgataatta caagatagaa
    16861 gatagcaagg attgcataca tcttgaaact ttggtagtta cgagcaatga taatcttacc
    16921 agtttggaag agttcaacca aaccgatagc agatacgata gttgtatctt taagagcgat
    16981 aacgaattgg ttgacaaagt ttggcaacat caatttagtt gcttgtggca agataatctt
    17041 acgcatggtt tttccataag agatacccaa gcttcggctg gcttccattt ggccaactgg
    17101 aacggcctga ataccaccac gaacgatttc agcgatataa gcagccgcat tgagtgagag
    17161 ggcaattgtt ccagctacaa agtcgttaat tgggctttgt tggcctgtga tagactcgat
    17221 gaagtttgga attccccaga agatgaaggc tgcaagaatc atcaatggaa taccacgaat
    17281 aacgtcaacg aaaatctcag agatgacgcg aagagatttg tatgggctaa cgctaaacat
    17341 accgaagata attccgatga caatggcaat agcaaatgag ataagagcta gagcaagagt
    17401 gataccaaga ccgctaagga gttgtttgta gttgttttga agcaagcccc agagcgttgt
    17461 ttcgtcaaca gtacttgttg aagcagttga agattcgcta gctaggtatt tgtcaagaat
    17521 cttttggaat tcaccgtttg ctttaaggtt tgcaagtccg ttgttgaaca tttcaatcag
    17581 ttctggattt gctccttttt taacggcaaa ggctgtttca ccgattggag ttccagagat
    17641 tggagttttc aatttttgac cttggctgat agaatatttg agaacaggtt catcatccat
    17701 aacggcatca atggcaccag tgtttaaact gtcatacatt gaagaaccat cagcaaaggt
    17761 tttgattttg taaccgtatt tgctttgatt ttctgttagg aaggtttgag aagcagttcc
    17821 gtttttaaca ccgactgtct ttccttttag atcttcataa gaagcaatat tgcttgattc
    17881 tttgacacca agaatggtat tagcagtgta gtatgattct gagaagtcaa aagttgcctt
    17941 acgagcatct gtgacagaca taccagcaat gataccatcg gcttgaccag cttggacagc
    18001 actgatagca gcatcaaaac cagggttggt gatttcaatt tcaaaacctt ggtctttagc
    18061 gattgcctta atcaattcca tatcaatacc agtgtattgg ttgcttgaat tttggaaaac
    18121 aaaaggtgca aaagaagaat cgctggcaat gatatattta gccttaacag gaatagcttt
    18181 taatcctgcg agagtagttg tagttggcac tgctgaagat gatgaagcag tccatttctt
    18241 gataatttta tcaagactac catctttttt catttcagcc aaggcttggt taaattcagt
    18301 aaccaggtgc tcgtatttac ttcctttttt cacaccgaaa gcaaaacttc ctacagcttc
    18361 accatccatt tcaatatgga ggtcttgacc ttggttaatg gcatattcga taacaggttt
    18421 gtcatccatc atggcatcga tggcaccagc actcaagctg ttgttcatta aatccccagt
    18481 gtcaaatgtt ttaatagtaa agccgtattt atctttgatt gtttcaagga aacgttgagc
    18541 ggcagttccg tttttaacac caacggtttt gccagttaat tggtcgtact tgctaatttt
    18601 gtgtgacttt gtagtagcaa tgacaacttt tgtatcatag taagtatcag acatggtgaa
    18661 gactttttca cgttctttag tctttgtcat ccctgccatg atagcgtcgg cttgcccagc
    18721 ttgaaccgca ttgactgctg cgtcaaatcc aggataggac atctgaatgt tccagccttt
    18781 aatctcagcg actttgttaa taatgtcaac atcaattcct ttataagttt gatctgaatc
    18841 tttaaactca aaaggtgcat aggcggtatc agaaacaatc ttaatcgttt ctgctttggc
    18901 aatacctaat gagaaaattg ggaataaaat tagcaaaaat gctagaaatt ttttcttcat
    18961 ttttagtctc cttttccgaa tattctctca ttatatcaga aaaagtaaaa aaaggcaaat
    19021 ttttatattt ttcgtgtcag aaaatataac attggttttt ccttttcttt cttgaattgc
    19081 tattaaaaag tgctataata atagtatata tagaagaaaa gaggacgggg atatgaagaa
    19141 caaaagaata tttaaagact tccaagcttc aaaaatgagt ttaaacattt acacaagccc
    19201 cttgttagcc tttgtttttg tcttcatagg agagtttgtg gcttatactt tatatggtat
    19261 tagtttgtta gctctcatcg gacttgctag aaattttgga gaggctggtc aaaatcttgc
    19321 aagctacttg cagaccttgc atcagagctt gacggataaa acaagtgact ttcgtttaat
    19381 tttaggatta ctggcctttg gttttattct taacactgtg ttcagatgga caagaaaagt
    19441 tgagaaaaga cctattcgaa ccttgggatt ttatagagag aatttcctca gcaatcttct
    19501 gaaaggattt agtctaggcc tggcactttt tcttctgacc ttgttaggtt tggtggtctt
    19561 aggtcaatat cgtttggaat ccattcactt gaatccttat tctcttgcct ttgtcgtctt
    19621 tactatccca ttttggattt tacaggggac agcagaagaa gtggtggccc gtgcttggct
    19681 acttcctcaa ttggcctcaa gaaccaatct aaaactagct attcttatat ctagcctgtt
    19741 ctttaccctg cttcatatgg gcaattctgg cctcacccct ctatctctag taaatctctt
    19801 tttattcgga gttgccatgg ctctttacct tctcaaaact gatacagttt ggggtgttgc
    19861 aggtattcat ggtgcttgga attttgctca gggtaatctc tttgggattt tagttagtgg
    19921 tcaaccgtca ggaacgtctc tgatgacctt tttaccacaa ggcaatcaag attggctatc
    19981 aggtggttct tttggcatag aaggttccat tatgacaagt ctggtattac tactgctgat
    20041 tgtctatctt gctaataaat taaagaaaga aaatgaaagg atgtgacttc ggtccgtcct
    20101 tttcttcgtg aaaatactat aagtatgcta aaataggaat agcacatgga gagaggattc
    20161 ttatgatcaa tcacattaca gataatcaat ttaaactagt atcaaaatat caaccatcag
    20221 gagatcaacc ccaagctatc gagcagttgg tggataacat tgagggggga gaaaaagctc
    20281 agattctgat gggggcgact ggaacaggga agacctatac tatgagtcag gtcatttcta
    20341 aagtcaataa accaactctg gttattgccc acaataaaac tctggctggt cagctctatg
    20401 gggagtttaa ggaatttttc cctgaaaatg cagttgagta tttcgtatcc tactatgatt
    20461 attaccagcc agaggcctat gtcccttcta gcgataccta tattgagaag gatagttctg
    20521 tcaatgacga gattgacaag cttcgccact cagctacctc agcacttttg gagcgtaatg
    20581 atgttattgt cgtggcctca gtctcttgta tctatggttt gggttcgccc aaggaatacg
    20641 ctgatagtgt cgttagtctc cgtcctggtc tagagatttc tcgtgataaa ctcttgaatg
    20701 acttggtcga tattcagttt gaacgcaatg atattgattt ccaacgcgga agatttcgcg
    20761 ttcgtgggga tgtggtagag attttcccag cttcccgaga tgaacatgcc tttcgagtag
    20821 aattttttgg agacgaaatt gaccgtattc gtgaagttga ggctctgaca ggtcaggtgt
    20881 tgggagaagt ggatcattta gcgattttcc cagcgacaca ctttgtgacc aatgacgacc
    20941 atatggaagt tgccattgca aagattcagg ccgagttgga agaacaatta gctgtctttg
    21001 aaaaggaagg taaactgctt gaagcccagc gtttgaaaca gcggacagag tatgatatcg
    21061 aaatgttgcg tgagatgggc tataccaatg gggttgagaa ctattcacga cacatggatg
    21121 gacggagcga aggagagcct ccttatacgc tcttagattt cttcccagat gatttcttaa
    21181 ttatgattga cgagagtcac atgaccatgg ggcaaatcaa gggcatgtac aatggagacc
    21241 gttcgcgtaa agaaatgctg gttaattatg gcttccgatt gccgtctgct ttggacaatc
    21301 gtcctctccg tcgggaggag tttgagagtc acgttcatca gattgtttac gtttcagcga
    21361 cacctggtga ctatgaaaat gaacagactg agacagtgat tgagcaaatc attcgtccaa
    21421 cgggactctt ggatcctgaa gtggaagtcc gtccgactat gggacagatt gatgacctct
    21481 tgggtgaaat caatgcccgc attgaaaaaa atgagcgtac ctttatcaca actttgacca
    21541 agaaaatggc agaggacttg accgactact tcaaggaaat gggtatcaag gtcaagtaca
    21601 tgcactcgga tatcaagacc ttggaacgga cggagattat ccgtgacctg cgcttgggtg
    21661 tctttgatgt cttggtcgga attaacctgc tccgtgaagg aattgacgtt cctgaagtga
    21721 gcctcgtagc tattctcgat gctgataagg aaggtttcct tcgcaacgaa cgtggactca
    21781 tccagactat tggacgtgct gctcgtaaca gtgaaggcca tgtcatcatg tatgcagaca
    21841 cggttaccca gtctatgcaa cgtgctatcg atgaaactgc ccgccgtcgg aaaattcaga
    21901 tgacctataa tgaagagcat gatatcgtac cacagacaat caagaaagaa atccgtgacc
    21961 tgattgccgt gaccaaggca gttgctaagg aagaggacaa ggaagtcgac atcaatagcc
    22021 tcaacaaaca agagcgcaag gaactcgtca agaaactaga aaaacaaatg caagaagccg
    22081 tcgaagtgct tgactttgaa ctggcggctc agattcgtga tatgatgttg gaagtcaagg
    22141 tcttggatta ggggaatagt atgatttatt taagaaagtt aaagagagaa gatttgatgt
    22201 ctttatggga aatggcttat tcacaactta atccagtttg gaaacagtat gatgctccct
    22261 attatgatga ttatcagtat ttttcaaatt ttaaagaatt cgaactacaa aaatcagaat
    22321 ccattttaag caactcaaat cgccttggta tttttgttga tgataaacta gttgggactg
    22381 tttcgcgtta ttgggtatgt aaacaaacaa gatggatgga attgggaatt ggtatttatg
    22441 ataaaaaatt ctggaacact ggtattggga aagttgctat gttgcagtgg atagatagga
    22501 cgtttcagga ttacttggag ttggagcatc tgggtttgac aacttggtca gggaatcttg
    22561 gtatgatgaa acttgctgaa aaattaagaa tgaaaaaaga agctcatatt ccaaaagttc
    22621 gttattatca aggtaaatat tttgatagta ttaaatatgg tattttgaga gaagactggg
    22681 agaaaataaa tgacggttat tatcaaatca atggaaactc ctgaagagat agaaggtaaa
    22741 tcccttgttc actggcaaac gtggagagag gcttatgatg accttttgcc tgcagaattt
    22801 caggagacaa tgacattaga aagatgtcga ttctttagtc aaaagtatcc agaaaataca
    22861 ttgattgcga taggtgatct gaaggtagtt ggttttatca gttatggtaa ctttcgtgat
    22921 gagactattc aagctggtga aattattgct ttatatgttt taaaagacta ttacggaaaa
    22981 ggcatagcac aaaagttaat gaaagcagct ttgactgctc tgaatcattt ttctgaaatt
    23041 ttcttatggg tattgaaaga taacaagcga gccattgctt tctatcaaaa aatgggtttt
    23101 acttttgatg gacaagaaaa aatacttgaa cttggaaagc ctataaagga aaaacgaatg
    23161 gtgttctatt ctaaataatt ttaaaaaagt aaaagataat atctctggta gtaagtctga
    23221 aaatttaata aattagaaag cgagtaaatt tatgtcccgt tcccaattaa caattttaac
    23281 aaatatctgt ctgattgaag acctcgaaac tcagcgcgtg gtgatgcagt atcgcgcccc
    23341 tgaaaacaat cgctggtctg gttatgcctt tcctggaggt catgtagaaa atgatgaggc
    23401 ttttgcggag tctgtcattc gtgaaatcta cgaagaaaca gggttgacta tccaaaatcc
    23461 tcaacttgtc ggcattaaaa attggccact agatacaggt gggcgctata ttgtcatttg
    23521 ttataaggcg actgagttct ctggtaccct tcaatcttca gaagagggag aagtttcttg
    23581 ggtgcaaaaa gaccagattc caaacttaaa tctggcctat gatatgctac cattgatgga
    23641 aatgatggaa gctcccgaca agtcagagtt tttctatcct cgccgtacag aagacgattg
    23701 ggaaaagaaa atcttctagt cttttactaa ataacctagc tgatccaagg cctcctcgat
    23761 atagtggagg tcttgttgtg tttcggcttc gactaggtgg taatggatgc catctgtcaa
    23821 ttcagataga ggtctaaagt cagaatgctc gacttgttct agaaaatgtt gcacatctct
    23881 gcgacaagac agtttaagca aggtttcaat ttctccataa acgggatggt caatcaaggt
    23941 attctggaca tgcccaccat tatcgacaat agcaaggagt tcctgaccga tttcttcaac
    24001 ttcatgtttc actttgaaaa gtttgtgaac ataaggattg gtatcaattt gcttatagat
    24061 gtagccacga ttggtggata ggataggagc gccatcggct ctcaaaattg caatgtcctg
    24121 cacaatgacc tgacgtgtga catgaaaatg ttccgccaaa ctttggccat taagggcttt
    24181 aggagcttct ttcaagagtt ggagcagggc ttgtttgcga tcttttgtca tagcttttcc
    24241 ttttaacggc gttttcgaag cactttatag acagctagtg ctaatgtata gtctaccata
    24301 ctatggataa ttgtgccaaa tccaactagt acaaatagaa catagaacat attttctaca
    24361 ttggtacccg aagttgcgta aaaaaggaca caggctaata cttcagcaag ggcatgaaca
    24421 acagccaaaa caaaattgaa aacccaggaa gattttggtt tatctagggt atcggggaat
    24481 ttttgtaggt aaagagctcc taaagcacca aaagatatat gggaaaaagc ccgaaaaacg
    24541 ataaccatgg gatagccagc catcaaaaat ccaaaactag aggctaggat gacaaaaact
    24601 gccatcaagg gcgacaagaa catggctata aaaatagcga tgtggctccc caaagtatag
    24661 gaagcaggtg gaatgacaat cttgaaaggc ataacaattg gaatcaaaat cgcaatagcc
    24721 gttaaaaggg ctgtcattgt cataaattgt gtctttttcc gtgtattcac aagaatctcc
    24781 tttttaactg catatacact agtatggtac aataaaccag acaataaagc aagaatttac
    24841 ttgggtttat agatcatttt ttagttaaaa gttatagtag atggaaacta gaatagtcca
    24901 cctctacttc taaaacattg ttagcgattt gactgtcctg atcgatttgt cctgttctta
    24961 tttcgtttta ctatagtaaa gatttcatta aaaagaaact gtatagagca aaatctccac
    25021 cttcaggttt gaaagcggag attgttttta ttttttcaag gtttgtagtc gtggaacaat
    25081 ggctagggtt agaattgcag aaatgaccaa ttcagcaatt gaatttgttg agataacggt
    25141 tgccagaaga agttggatat ttccattata aacatttcca aataggaaga agattcctcc
    25201 aaggacaaag atagtatttg tcaaggaacc aagggctcca gctaaaatca gaccagtctt
    25261 gtttttcatc agtttataga ctaagtaagg agttaaacca atcaaaatac gtgggacgat
    25321 ggcaatgata gctgagtaga tgtttccgtt tggtacgaag ggagagaaga ggtagcttgt
    25381 cggtagaatc gtaatcgtgt taaccgtcaa gctaagtaat cccatcaaaa atccaagtgt
    25441 aaccccaact cgtggaccat aaataatgct ggcaataatg acaggaatat gaacaatggt
    25501 cggtttgatt ggaaatggaa aaaggttaaa gataagtgag ctcagaaagt gtatcacgag
    25561 catggtagca aaaaagatag caatgggtgc aatattagag cgttttttca tcgatagttt
    25621 cctttattct ttctaaaata attgtgaggt cagctaaagc tcctcgtccg tggtctccac
    25681 aagctagtag ggattcctta ggagcaatca gctgatagcc gtaggtttct aatgttttca
    25741 gattattctg agttactgga tggtcataca tttttgtatt catagcagga gctattagtt
    25801 tgggaatatg acttggtagg gctagagctg tactggttac catgttgtcc gcaaatccgt
    25861 gagctagttt tgcaatagtg ttagcagttg caggtaccac gataaataaa tctgcttttt
    25921 ttccaagttc gatatgattg acctgatcag gatagggttc cttcatgaca tccaagtgga
    25981 caggattctg tgagagtacc tgtagtgtca aaggttggat aaactctgta gcagcctgag
    26041 tcattaagac agtgacttga tggccttgtt tttttagaga actgactaaa tctgccgact
    26101 tataagaggc gattgaaccc gttacagcca agagaatgtt tgccatagtt ttcctttcta
    26161 agaatgatag gcttgaattt tttcaaggag gagttctgca atttcttctt tagtctggac
    26221 tgtttgaagc tgatttttct caacaaatat agctcggtgc tgatctgctg aaatttgagt
    26281 caggtcattc gcgatgatta aatctgcttg attcttgata agactttttc gtgcaatgtc
    26341 aaccagatga tcttcggtaa catcaaccag cagtttgaaa ccaatcagat gaatagtggg
    26401 attccattcc ttgactaggg atatgatttt gggtgtcttt ttaaggaaca aaacctgaac
    26461 ctcatcagtt gaagaaatct tggcctgatg attctgcttg cttaaaaatt cttttagatt
    26521 ggagctagcc tgaacttcct caagccctgt catataaaca ggagtgtagt cagaaacagc
    26581 cattgagtgg atcaagacct gataatcctg aacacgttct tgcatttcta ttagaaggtc
    26641 cttggtattg gtaatttctc gaatacttag gttaggatga ggctctggct tcagagctcg
    26701 ttttgtcgta attaaacaaa cttcatgccc tgcagaaagc aaagtctctg tgataatttt
    26761 ccccaagtga cctgtagaat ggttagtgat agagcggacg ctatcgatag cttcactggt
    26821 accgcccgat gtaactaaaa ttttcatagc actattgtac acaaaaataa gcggtaagta
    26881 aaatgaaagt gataaatttt tggtaaaaac gaagatttac tatagtttca aactataaag
    26941 ccatataaaa tttaagaaag gactctaaaa gccttaaaaa cagagatatt tacgaacgtt
    27001 tagagagttt taggatgtta aaaatcttgt tttgtgatat aatgaattat catataagag
    27061 gttagagaaa ttttgaatga aaacagatat tgaaatcgca cagagtattg agttgaagcc
    27121 aattgttgat gttgtagaga aacttggtat ttcttatgac gatttggagt tgtacggaaa
    27181 gtacaaggct aaactcagct ttgataaaat tcgtgcagtt gagagcaatc cagtcggtaa
    27241 attgatcttg gttactgcca tcaacccaac acctgcaggt gaaggaaagt cgacgcttac
    27301 cattggtctt gcggatgcct tgaacaagat tggcaagaaa accatgattg ctatccgcga
    27361 accgtctctt ggtccagtca tggggatcaa gggtggtgct gctggtggtg ggtatgcaca
    27421 agttctgcca atggaagaca tcaacctcca ctttactgga gatatgcatg ctattacaac
    27481 tgccaacaat gccctttctg ccttgattga caaccacttg caccaaggga atgagctgga
    27541 aattgatcaa cgtcgtatcc tctggaaacg cgttgtggac ttgaacgacc gtgcgctccg
    27601 ccatgtgact gttggtcttg gtggtcctct aaacggtatt ccacgtgagg atggttttga
    27661 tattacagtt gcttcagaaa tcatggcaat tctttgcttg gcaacggaca tcgaggactt
    27721 gaaacgtcgt ttggcgaata tcgttattgg ttatcgctat gaccgtacgc ctgtttctgt
    27781 aggtgatttg caggttgagg gtgccttggc tttgattttg aaggatgcta ttaagccaaa
    27841 cttggttcag acaatttacg gtacacctgc ctttgtacac ggtggtccat ttgccaatat
    27901 cgctcatggc tgtaactctg ttttggcgac gacaacagcc cttcacttgg ctgattacac
    27961 tgttactgaa gctggttttg gtgcggacct tggtgctgag aaattccttg atatcaagac
    28021 accaaacttg ccaacatctc cagatgcagt tgtcattgtc gcaacccttc gtgcccttaa
    28081 gatgaatggt ggtgtggcta aagacgctct gactgaagaa aatgtagagg cagttcgtgc
    28141 aggttttgct aacttgaaac gccacgttga aaatatccgt aagttcggta ttccagcagt
    28201 tgtagctatt aacgaatttg tatctgatac agaagctgaa attgcagtct taaaagaact
    28261 ctgtgcctca atcgatgtac cagttgaatt ggctagtgtc tgggctgatg gagcagaagg
    28321 tggagtagca cttgccgaaa cagttgttaa gacaattgct gaaaatccag ctaactataa
    28381 acgtttgtac gataatgccc tttctgtcca agaaaagatt gaaaaaattg tcactgaaat
    28441 ctatcgtggt agcaaagtga actttgagaa gaaagctcaa acacaaattg ctcaaatcgt
    28501 tcaaaacggt tgggacaaat tgccaatctg tatggctaaa actcaataca gtttctcaga
    28561 caatccaaat gcgcttggag caccagaaaa ctttgaaatt accattcgtg aattggtacc
    28621 aaaattaggt gcaggcttca tcgttgcctt aactggtgat gttatgacca tgccaggtct
    28681 tccaaaacgt ccagcagctc tcaacatgga tgttgaaagc gatggaactg tactaggctt
    28741 gttctagtgg attgaaattg actttaagtg agtttggaaa taatatccaa gctcattttt
    28801 tctcagactg caagcagttc atcaataaga tttatactct tcgaaaatct cttcaaac
//
LOCUS       DAVBCW010000016        28502 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVBCW010000016 DAVBCW010000000
VERSION     DAVBCW010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28502
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.016"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            47..535
                     /locus_tag="VQ514_000592"
     CDS             47..535
                     /locus_tag="VQ514_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HET2499391.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKIIFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            532..1719
                     /locus_tag="VQ514_000593"
     CDS             532..1719
                     /locus_tag="VQ514_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET2499392.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAAVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTVILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            1712..2077
                     /locus_tag="VQ514_000594"
     CDS             1712..2077
                     /locus_tag="VQ514_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET2499393.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEVQACYEAGLEAGGRDNGAPGYRSEHPIYY
                     AAFMIDLDGNNIEVVCHKE"
     gene            2093..3439
                     /gene="asnS"
                     /locus_tag="VQ514_000595"
     CDS             2093..3439
                     /gene="asnS"
                     /locus_tag="VQ514_000595"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HET2499394.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            3592..3882
                     /locus_tag="VQ514_000596"
     CDS             3592..3882
                     /locus_tag="VQ514_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HET2499395.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            3894..4364
                     /gene="ssbA"
                     /locus_tag="VQ514_000597"
     CDS             3894..4364
                     /gene="ssbA"
                     /locus_tag="VQ514_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HET2499396.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            4396..4635
                     /locus_tag="VQ514_000598"
     CDS             4396..4635
                     /locus_tag="VQ514_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HET2499397.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            4930..6330
                     /locus_tag="VQ514_000599"
     CDS             4930..6330
                     /locus_tag="VQ514_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HET2499398.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSYVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDVIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            6332..6691
                     /locus_tag="VQ514_000600"
     CDS             6332..6691
                     /locus_tag="VQ514_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HET2499399.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            6745..7494
                     /locus_tag="VQ514_000601"
     CDS             6745..7494
                     /locus_tag="VQ514_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HET2499400.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPYSNLNESEHYLLARHEITTNLEEICHSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            7484..7783
                     /locus_tag="VQ514_000602"
     CDS             7484..7783
                     /locus_tag="VQ514_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HET2499401.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDMRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALFKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            7854..8789
                     /locus_tag="VQ514_000603"
     CDS             7854..8789
                     /locus_tag="VQ514_000603"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HET2499402.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            8876..9388
                     /locus_tag="VQ514_000604"
     CDS             8876..9388
                     /locus_tag="VQ514_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HET2499403.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGAVCQEPADFFESNHLYDLYVYPEMDIQSVKERACRHLGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            9425..10020
                     /locus_tag="VQ514_000605"
                     /pseudo
     CDS             9425..10020
                     /locus_tag="VQ514_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
     gene            10121..10315
                     /locus_tag="VQ514_000606"
     CDS             10121..10315
                     /locus_tag="VQ514_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET2499404.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGAVEGLKNVFTK"
     gene            complement(10361..11806)
                     /locus_tag="VQ514_000607"
     CDS             complement(10361..11806)
                     /locus_tag="VQ514_000607"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HET2499405.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVIFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVDIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTATYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQAYLVHRVYGLTFDVGVFLNITPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            11912..13534
                     /locus_tag="VQ514_000608"
     CDS             11912..13534
                     /locus_tag="VQ514_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HET2499406.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            13738..15696
                     /locus_tag="VQ514_000609"
     CDS             13738..15696
                     /locus_tag="VQ514_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2499407.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGFLQNLGLEPGEANDKAKAVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFATAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            15868..16803
                     /locus_tag="VQ514_000610"
     CDS             15868..16803
                     /locus_tag="VQ514_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2499408.1"
                     /translation="MKLKLLRVDTKVIMGSFLLVLSSLLALLLPLILKGLIDGSSIEN
                     IGSKVFQSFLIFIGQALFSSIGYYLFSQSGEKKIAKIRKKVIEGLIYAEKSFFDKSQS
                     GELTSAIVNDTSVIREFLITTFPNIILSLVMVLGSIVVLFSLDWNLSLLLFITLPCMM
                     FIILLLSNISEKYSRRLQEEIGFLTGQLTEKIQEHELIKTNQAEKSVQDVLDNCIERV
                     QNNSLKSDRVTSFETPFALLFIFATIAVMLTYGGYRVSAGYISVGTLVSFLIYLFQLL
                     NPISNIANFVTVYSRSKGSSVALENLLAVPKEKFE"
     gene            16828..17480
                     /locus_tag="VQ514_000611"
                     /pseudo
     CDS             16828..17480
                     /locus_tag="VQ514_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864981.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            17638..18732
                     /locus_tag="VQ514_000612"
     CDS             17638..18732
                     /locus_tag="VQ514_000612"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HET2499409.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGRFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQALEG"
     gene            18740..19906
                     /locus_tag="VQ514_000613"
     CDS             18740..19906
                     /locus_tag="VQ514_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET2499410.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEIQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDGLFEIDFDQLEKDLVEEE
                     VKLYILCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKR"
     gene            19999..23097
                     /locus_tag="VQ514_000614"
     CDS             19999..23097
                     /locus_tag="VQ514_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2499411.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDSFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLNRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQNIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPLQATQVKADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSEFYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            23145..23762
                     /locus_tag="VQ514_000615"
     CDS             23145..23762
                     /locus_tag="VQ514_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HET2499412.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPIAEEKPVKPIEKQVGKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            23772..25106
                     /locus_tag="VQ514_000616"
     CDS             23772..25106
                     /locus_tag="VQ514_000616"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HET2499413.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFSGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            25116..25580
                     /locus_tag="VQ514_000617"
     CDS             25116..25580
                     /locus_tag="VQ514_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2499414.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQYLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     DLDYKGIRLESPDELLSYFEMNGFVDEEATLLYATSQGYSMIWFNPFYLEEQ"
     gene            25577..26071
                     /locus_tag="VQ514_000618"
     CDS             25577..26071
                     /locus_tag="VQ514_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2499415.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            26152..27801
                     /gene="mltG"
                     /locus_tag="VQ514_000619"
     CDS             26152..27801
                     /gene="mltG"
                     /locus_tag="VQ514_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HET2499416.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMTDSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDIIS
                     DTSRRSRREGAKPVNPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPIDA
                     NSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQKSM
                     STEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQAVGQLQGDFKESLTAEAFLA
                     KVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDEML
                     AAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDMPL
                     QSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIESSI
                     NQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            27872..28354
                     /gene="greA"
                     /locus_tag="VQ514_000620"
     CDS             27872..28354
                     /gene="greA"
                     /locus_tag="VQ514_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HET2499417.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
BASE COUNT         8544 a         5318 c         6418 g         8222 t
ORIGIN      
        1 cttatattca cttgtaataa agcttaatag atggtaaaat agacgagtga aaaaaagaca
       61 aaacaaagca aaaaataatc tactgtggca gtatggtcta gggatgacga ttttgtttgt
      121 ggttatcagt gcttcctttc tgtatatggt ttctcttagc atgaaaccct atcaaacagc
      181 taaaagtgaa ggagaaaaat tagctcagca gtatgcagga ttagagcagg ccgatcaggt
      241 tgatttatac aatggcttgg aatcttatta cagcgttctt ggtcgtaata aacagcaaga
      301 agcacttgct gttctgattg gaaaagatga tcataagatt tacgtttatc agctaaatca
      361 gggtgtttca caagaaaaag cagaaacggt ttctaaggaa aagggagctg gcgaaattga
      421 caagattatc tttggtcgtt atcaagataa gccaatctgg gaagtcaagt caggatctga
      481 tttttatcta gtagattttg aaacaggagc attggtcaac aaggagggcc tatgaaatta
      541 tccaaccgtg ttttagaaat ggaagaaagt gtgactttgg ctgctggagc ccgtgccaaa
      601 gcactgaagg ctgagggtag agatattttg tctctaacct tgggtgagcc agatttcact
      661 actcccaaaa atatccaaga tgccgccatt gcatcgattc gagatgggcg tgcttctttt
      721 tatacagtaa cctcaggtct gccagagctt aaggcggcgg tcaatagcta ctttgagcgc
      781 ttttacggct attctgtagc gtcaaatcaa gtgacagtcg ctgcgggagc caaatattct
      841 ctctatacct tctttatggc tgtggtcaat ccaggtgatg aagtcatcat cccaacccca
      901 tactgggtta gctatggaga tcaggtcaag atggcagaag gcgttcccgt ctttgtttct
      961 gctaaggaag acaatcactt taaggtgacc gtagagcagt tagaagcagc tcgcactgac
     1021 aagaccaagg ttttggtgct gaattcgcca tctaatccca caggtatgat ttacacccgt
     1081 gaggaactct tggcaattgg aaactgggct gtagaaaatg atattctcat cctagcagat
     1141 gatatctatg gccgcttggt ttataatggt catgagttca caccgatttc tagcctatcg
     1201 gaagcgattc gcaagcaaac agtggtcatc aatggtgtgt ctaaaaccta tgccatgact
     1261 ggttggcgga ttggttatgc cgttggagaa gcagacatta tcgctgccat gtccaagatt
     1321 gcaggtcaaa caacttcgaa tccgtcagca gtagcccaat atgcagcagt tgaggctcta
     1381 tcaggtgagc aagatactgt agaaagcatg cgtcaggcct ttgaagaacg tcttaatacc
     1441 atctatcccc tccttgcaga agtgccagga tttgaagtgg tcaaaccgca aggggccttc
     1501 tatctcttcc caaatgtcaa aaaggccatg gagatgaaag gctacacgga tgtgacagac
     1561 tttacaactg ttatcttaga agaagccgaa gtggccttgg taacaggagc tggatttgga
     1621 gcaccagaaa atgtccgcct cagctatgcg acagacctag acacgcttaa agaagcagtc
     1681 gaacgcttga aagcatttat gggtagtgag aatgattgat cattttgaga ttaaggtaaa
     1741 ggatttacaa atttcagaag gattttatag gagttttctc gctcctttgg actataaatt
     1801 ggcttttaag actagttctc taattagttt tctatccccg aacagccctc atcctggtgg
     1861 tgatttttgg ctgactcagg gaacacaaga tcctgttcat tttgcttttt tagcagaaaa
     1921 taaggaagaa gttcaggctt gttatgaggc tggcttagag gcaggtgggc gagacaatgg
     1981 ggctcctggt tatcgaagtg agcatccgat ttactatgct gcttttatga ttgacctgga
     2041 tgggaacaat atagaggtgg tttgccataa agaataaaaa agaaaaggaa aaataatgac
     2101 aaaacgtgta acgattattg acgtaaaaga ctatgttggt caggaagtga cgattggcgc
     2161 ttgggttgcc aacaaatcag gaaaagggaa aatcgctttc ttacaattgc gtgatggaac
     2221 agccttcttt caaggtgtgg cttttaaacc aaactttgtc gaaaaatttg gtgaagaagt
     2281 gggacttgag aagtttgatg ttatcaaacg cttgagccaa gaaacgtctg tttatgtgac
     2341 aggtattgtc aaagaggacg aacgttctaa atttggctat gagttggaca tcacagacat
     2401 cgaagtgatc ggtgaatctc aagactaccc aatcacacca aaagaacacg gaacagactt
     2461 tttgatggat aaccgtcact tgtggctacg ctctcgtaag caagtagctg tgttgcaaat
     2521 ccgtaacgct attatctatg caacttatga gttctttgac aagaacggtt ttatgaagtt
     2581 tgacagccca attctttcag gaaatgcggc agaagattct acagaactct ttgaaactga
     2641 ctacttcgga acgccagcct acttgagcca atcaggtcaa ctttacctag aagcaggggc
     2701 tatggctctt ggtcgtgtct ttgactttgg tccagttttc cgtgctgaaa aatcaaaaac
     2761 acgccgtcac ttgactgagt tctggatgat ggatgctgag tactcatact tgacacatga
     2821 tgagtcgctt gacttgcaag aagcttatgt gaaagctctt ctacaaggtg ttcttgaccg
     2881 cgcgcctcaa gccttggaaa ccttggaacg tgatacagaa ctcttgaaac gctacattgc
     2941 agagccattc aaacgtatca cttacgatca agccattgac ctcttgcaag agcatgaaaa
     3001 tgatgaagat gctgactacg agcatcttga gcatggtgat gactttgggt caccacacga
     3061 aacttggatt tcaaaccact ttggtgtgcc aacatttgtc atgaactatc cagcagccat
     3121 caaggccttc tacatgaaac cagttcctgg aaatccagag cgcgtgcttt gtgcagactt
     3181 gcttgctcca gaaggctatg gagaaattat cggtgggtct atgcgtgagg aagattacga
     3241 tgcccttgtc gctaagatgg atgaacttgg catggatcgt acagaatatg aattctacct
     3301 tgaccttcgt aaatacggta cagttccaca cggaggattt ggtatcggta tcgaacgtat
     3361 ggtaaccttc gcagcaggaa caaaacatat ccgtgaagct attccattcc cacgtatgtt
     3421 gcaccgtatc aaaccataag aacaggaaaa cgcccatgtg gcgttttttc gaattctagt
     3481 gtttttactt gcgtaaaaaa aatttttcta gtataatagt ttattgtgag cgaacctcac
     3541 ttaccccttg caaagtcttg gggtcattag accaaaagga ggaacatatc aatggctaaa
     3601 tacgaaattc tttatatcat tcgtccaaac attgaagaag aagctaaaaa cgctttggta
     3661 gcacgttttg actctatttt gactgacaac ggtgcaactg ttgttgaatc aaaaacttgg
     3721 gaaaaacgtc gtcttgcata cgaaatccaa gatttccgtg aaggacttta ccacatcgtt
     3781 aacgttgaag caaatgacga tgcagctctt aaagagtttg accgtctttc aaaaatcaac
     3841 gctgacattc ttcgtcacat gatcgtcaaa attgacgcgt aagaaggtaa actatgatta
     3901 acaatgttgt acttgtaggg cgtatgacac gtgacgctga attgcgttat accccatcaa
     3961 atgtagcagt tgcgactttt actcttgcag taaaccgtac atttaagagt caaaatggtg
     4021 aacgtgaggc tgattttatc aatgtcgtta tgtggcgcca acaggctgaa aatcttgcta
     4081 actgggctaa aaaaggctca cttatcgggg tgacaggtcg tatccagact cgtagttacg
     4141 ataaccagca aggacaacgt gtctacgtga cagaggtcgt ggctgagaat ttccaaatgt
     4201 tggaaagccg tagtgtgcgt gagggtcaca caggtggagc ttactctgca ccaactgcaa
     4261 actattcagc acctacaaat tcagtaccag acttttcacg taatgaaaat ccatttggag
     4321 caacaaaccc attggatatt tcagatgatg atttaccatt ctaatggaca actaaactta
     4381 taaaggagaa aaaacatggc tcaacaacgt cgtggcggat tcaaacgccg taaaaaagtt
     4441 gattacatcg cagcaaacaa aattgaatat gttgattaca aagatactga gcttcttagc
     4501 cgtttcgttt cagaacgtgg gaaaatcctt cctcgtcgtg taacaggaac ttcagctaaa
     4561 aaccaacgta aagtaacaac agctatcaaa cgcgctcgcg taatggcttt gatgcctttc
     4621 gtaaacgaag attaaagaaa agacccggtt gggtctcttt ttcatgaact tgaaaacaag
     4681 aaagcgaatt aagaccccaa cgggtctttt ttcacaagct ttgaaatgag agagcgagtt
     4741 aggctcagtg gctccctttt tacgagtttg aaaacaagag aacacattaa aagcacgatg
     4801 gagctttttc ataaatatgt cttttttttc atccaagaaa ttaaaataaa aatgataaac
     4861 tttttgaaaa taggggattt gcctacttta tgatataatg gaaagagtta gattgaaaga
     4921 ggtagtgaga tggacgcgaa attaagatac aaggcaaaga agatcaagat tgtctttttt
     4981 gatattgatg atacattgcg gaattcaaag acagggttta ttccaactac aattcccact
     5041 gtttttaaac agttgcgtga aaaaggaatt ttaacaggaa tcgcctctgg acgtggcatt
     5101 tttggtgttg ttccagagat tcgtgatctc aaacctgact tttttgtaac tttgaatggg
     5161 gcttatatcg aagataaaaa aggtcaggtc atttatcagc atcagattga aaagtcatat
     5221 gttgaggagt atatctcttg ggctaagcaa gaaggaattg agtatggctt ggttgggagt
     5281 catgatgcca agttgtcgac tcgcaccgat atgatgagtg aagctatcaa tccaatttat
     5341 cccgacttag atgtagatcc cgatttccat gaaaaagaag atatctatca gatgtggact
     5401 tttgaagata agggagatga cttgcacttg cctgacagtc tctcagacaa acttcgcatg
     5461 gttcgttggc atcaacattc gtctgatatt gtgccgattt caggctccaa agcgacgggg
     5521 gtggaaaagg ttgtggaaca ccttggcttg aaaccagaga aggtcatggt ttttggagat
     5581 ggtctcaatg acttggaact ctttgattat gctggaatca gcgttgccat gggaatttct
     5641 catgataaaa tcaaagaaaa agcagattat attacaaaaa cattagaaga agatggcatt
     5701 tttgatgcct tagaagtatt tggtatggta gaaaaagaat tgcattttcc acaagtagac
     5761 attgaaacag tagaaggtcc tcttgcgact attaagacca atcacggaga cttacgtatc
     5821 aagcttttcc ctgaacatgc tcctaaaaca gtggctaact ttgtatctct ttcaaaagat
     5881 ggctactatg atggtgtcat tttccaccgt attatcaagg actttatgat ccaaggtgga
     5941 gacccaactg gaactggtat gggtggcgag tcaatctacg gcgaatcttt tgaggatgaa
     6001 ttctcagaag agctttacaa tatccgtggt gctctttcca tggcaaatgc tggtccaaat
     6061 accaacggca gccagttctt tatcgtgcaa aaccaacacc taccttattc taagaaagaa
     6121 attactcgtg gtggttggcc agaaccgatt gcagaaatct atgccaatca aggtgggaca
     6181 cctcacctag accgccgtca cacggttttt ggtcagttag ctgatgaagc atcttacgct
     6241 gtcttggatg tcattgctgc tgttgagaca ggagctatgg acaagccagt tgaagatgtt
     6301 gtaattgaaa ctattgaaat cgaggactaa gatgaaaatc ggtgataagc taaaggggcg
     6361 tattacaggg attcagccct acggtgcctt tgttgagtta gagacgggtg atacggggct
     6421 gattcacatt tcagaaattc ggacaggatt tattgaaaat attcatgaaa ccttgaaagt
     6481 cgatgaagag gttcaagttc aggtagtgga tttagatgaa tttacgggga aagccagtct
     6541 ttctatccgc actttggagg aagaaaagta ccagtttcct agacggagac gcttttcaag
     6601 cgaccgcttt aactacggtt ttgctccctt tcgacgaatg ctaccaatct ggactggaga
     6661 agccctgcac catttaaaaa agaagaagta gtttagagtt ttatattttt tgtaatgtta
     6721 atataaattt gctataatag gaccatggaa gaagaacaat tattaaaatc aggagagcgc
     6781 attaaccagc tcttttcgac agatatcaaa atcattcaaa atagagaggt ttttagctat
     6841 tcggtggata gtgttctctt atcacgattt ccacgttttc ctaagaaggg gttgattgtg
     6901 gatttctgtg ctgggaatgg agcagtgggg ctttttgcta gcactcgtac tcaagcacag
     6961 atattgtctg ttgagattca ggagcgtttg gcggatatgg ctgaacgctc tgtccgtttg
     7021 aatggattag aggagcaaat gcaggtcatc tgcgatgatt tgaaaaatat gcctgctcac
     7081 attcagggga gtaaggtgga tatgattttg tgtaatccac cctatttcaa ggtggatcct
     7141 tattctaatc tgaacgagag tgaacattat ctcttggctc gacacgaaat cacgactaat
     7201 ttggaagaaa tctgtcatag tgcccagagt attctcaagt ctaatgggcg tttggccatg
     7261 gttcatcgtc ctgatcgact tctggatatt ttggatacgt taaaacgaca taatctagcc
     7321 cctaagcgcc tgcagtttgt ttatccaaaa agagaaaagg aagccaatat gcttttgatt
     7381 gaggcgatca aggatggctc aacaagtggc tttaaggtct tacctccttt gattgtccac
     7441 aatgatgatg gctcttatac gcccgaaatc gaagagattt attatggatc ataaggctta
     7501 tatgtacgtt ctggagtgtc gtgatggatc ctactatata ggctatacga ctgatatgag
     7561 aagacgcctc gctatccaca atagtgggaa gggagccaaa tatacacgag cacgcttgcc
     7621 agtcaaactt atctatgctc aaggttttgc cagtaaggaa gaagccatgt cggctgaagc
     7681 tcttttcaag cgtaagaaga ggccacagaa ggaagaattt ttatctgaaa atcaagatag
     7741 aaatttactc agttattttg aagaatcgtg gggagtcctt tgactcctct tacctttgtc
     7801 aaaaagcgaa aaaaatgcta caataaagaa aataaacaaa atgaggtatt atcatgtcca
     7861 agattctagt atttggtcac caaaatccag actcagatgc catcgggtca tctgtagctt
     7921 ttgcctacct tgcaaaagaa gcttacggtt tggatacgga agctgttgcc cttggaactc
     7981 caaatgaaga aacagccttt gtcttgaact attttggtgt ggaagcacca cgtgttatca
     8041 cttctgccaa agcagagggg gcagagcaag ttatcttgac tgaccacaat gaattccaac
     8101 aatctgtatc agatatcgct gaagtagaag tttacggtgt tgtagaccac caccgtgtgg
     8161 ctaactttga aactgcaagc ccactttaca tgcgtttgga gccagttgga tcagcgtctt
     8221 caatcgttta ccgtatgttc aaagaacatg gtgtagctgt gcctaaagag attgcaggtt
     8281 tgatgctttc aggtttgatt tcagataccc ttcttttgaa atcaccaaca acacacccaa
     8341 cagataaaat cattgctcct gaattggctg aattggctgg tgtaaacttg gaagaatatg
     8401 gtttggcaat gttgaaagct ggtaccaact tggctagcaa atctgctgaa gaattgattg
     8461 acatcgatgc taagactttt gaactcaacg gaaataatgt ccgtgttgcc caagtgaaca
     8521 cagttgacat cgctgaagtt ttggaacgcc aagcagaaat tgaagctgca atgcaagctg
     8581 ccaacgaatc aaacggctac tctgactttg tcttgatgat tacagatatc gtcaactcaa
     8641 actcagaaat attggctctt ggtgccaata tggacaaggt cgaagcggct ttcaatttca
     8701 aacttgaaaa caatcatgcc ttccttgctg gtgccgtttc acgtaagaaa caagtggtac
     8761 ctcaattaac tgaaagcttt aatgcgtaag attttgggtg tcagctcaaa atcggaaagt
     8821 ctagtttgcc ttatatcgaa aggagtttcg gctccttttt ttctaggagt gaagtatgtt
     8881 agaaaatggc gatttgattt ttgtgagaga tgggtcagac atgggacagg ccatccagac
     8941 ttccacaggt aactatagcc atgttgccat ttatttggat gggatgattt atcatgctag
     9001 tggacaggct ggtgctgtct gtcaagaacc ggcagacttc tttgagtcca atcatttgta
     9061 cgacctctat gtttacccag aaatggatat ccagtcggtg aaggaaagag cttgcagaca
     9121 tcttggagca ccctacaatg cttctttcta tccagatgca gctggtttct actgctccca
     9181 gtatatagca gaaatcctac ctatttttga aactattcct atgaaatttg gagatgggga
     9241 acaggagatt agtgattttt ggagggagta ttacatagaa ctaggtctgc ctgttcctct
     9301 gaaccaagct gggaccaatc ctagtcagtt ggcagcatcg cctctgttac aatgtaaaga
     9361 aaggaatctt catgattcag atttttaatt catctcgttt gacgagacag ccatttttgg
     9421 agaattgatc cgctatctgg accagtatga ggatgtgatt ctacgggaaa ttaaggctca
     9481 atttccagat gttgcagttg ataaactcat ggaagagtat ataaaggcaa gcttgattct
     9541 acgtgaaaat aagcgctatt acctcaattt tcctacgctt gaatcacttg atagtcttga
     9601 actggatcaa gagatttttg tcagagaagc tagtccggtc tatcaagcct tgttggagca
     9661 gagttttgag acggaattgc gcaatcaaat caatgcagct attttagttg aaagacggac
     9721 tttgcgcgca ttaaaatgac cctgtccaat tatttttaca aggtcaaaca gcagtatcct
     9781 ttgacagaaa aacagcagga gctctatgac attttaggag atgttaatcc tgagtatgca
     9841 ctcaagtata tgacggcttt tttgttgaaa tttctcaaaa aagaccagct tatgcagaaa
     9901 tgccgtgata tctttgtgga cagtttggtt gtcttaggct atattgtgca aaatgaagat
     9961 agaaagtatg agttggctat cgattttgat aaggagagat taactttcta cttagcgtga
    10021 tttcttgttt ctgagcacat tgtttgactt tccttagtat tcggtataaa ctatatgtaa
    10081 ccggtaacac atatcggaat aaactaaagg agacaatcat atgtcacttg aaaacaaatt
    10141 ggaacaagca acaggcgctg tcaaagaagg ttttggtaaa gttactggag acagcaagac
    10201 agaacttgaa ggagctgttg aaaaaacagt tgctaaggca aaagacgttg tagaagacgc
    10261 aaaaggtgct gtagaaggtg ccgttgaagg tttgaaaaac gtttttacta aataatagga
    10321 aaaaatcaag ggtttcattt tcccttgatt ttttctattc ttataaataa ttttctgcga
    10381 cggctgtatc tcctgggtag gattctttct tgccctggat gatttggtaa caatcggctc
    10441 ccttacccgc aataataact gcatctaatt cgtgatttgt gatagccatt gccgccttga
    10501 tggcttcttg gcgatccgca atcttttcaa caggatgatt gatgtagcta ctaatttcat
    10561 ctgcaatggc cattgggtct tcatagttag ggtcatcagc agtcagaaag acttgaatct
    10621 cagggtgttg attgaggagg aggccaaagt ccttacgacg actttctccc ttgtttcctg
    10681 ttgatcccag aaccagagca atctttccgg tttgatgagt ttcaaccaca ttgatgagtt
    10741 ttttcagact atccccattg tgggcatagt cgatgaagac cttggctcca tttttctgag
    10801 tgaggacttc catacgacca ggaacgcggg ttgcagcgat gccttttttg atgtcctcaa
    10861 gacttgctcc gagacggaga caagcaagtc cagcagcaac tgcattttct tggttgaagt
    10921 tgccaatgag ttggatatca taatctccag cgagtttacc cgtagctgaa aagctaaagg
    10981 ctttggaatt ctcgatttgg ttatcaaatt ggctaccata gaaatcatgg tcttgatctt
    11041 caacctgttc tttcaagact gagaagtggt ccatgtcact gttaatgatg actgctcggc
    11101 tattttccat caagagacgc ttgtggtaga aatagtcttc aaagctaggg tgttcaatcg
    11161 ggccgatatg gtcaggagtg atgttaagaa agactcctac atcaaaggtc agtccataga
    11221 ctcgatggac tagataggct tgactggaga cttccatgat gaggtgggta cggtcatttt
    11281 gcacagcctg attcatcatg tcaaagaggt caatactctc aggggttgtc aacgctgact
    11341 taaagaaagt ctcgccatca agagttgtgt tcatggtcga caacatagca ggtctatgcc
    11401 cttgagataa gatgttatag gcgaaatagg ttgctgttgt cttaccctta gtaccagtaa
    11461 aggcaaggag tttgagtttc tcttgtggat taccatagaa ctccatggca atcaaactca
    11521 tggctttctt tatatcgttc acaatgatga cagggatatc gacttcgtag tccttttcag
    11581 ctacatacca agctaaacct tgtgttatag cagaaagaag gtattctttt ttaaaggcag
    11641 cgccttttgc aaaaaaaaga gtgtcttctg ttacttttcg gctgtcgtag ctgatgctat
    11701 caaaaataac tttgctgtag ttgtagtggt aatgaccttg gtcaataatt tcgcgaaaaa
    11761 ggccatcttt ctttaaaata tctaatacgg tttcaatctt aatcatactt tctattgtaa
    11821 accgaaagtc gtaaatttac aagtaacaag gaaaagttta taatggaaga taaggagttt
    11881 ttcctagtta tcaaaattga atgaggaatc tatgtcgcac gaaaacaatc accagcaggc
    11941 ccagatgtta cgggggactg cttggctaac ggctagtaac tttatcagtc gcctactcgg
    12001 ggctgtttac attatccctt ggtacatctg gatgggggct tatgcagcta aggcaaatgg
    12061 tctctttacc atgggttaca atatctatgc ttggttcttg ttggtttcaa cagcggggat
    12121 tccagttgcg gtggccaagc aagttgccaa gtataatact atgcgagaag aagagcatag
    12181 ctttgccctg attcggagct tcttaggctt tatgacagga ctaggcctgg tttttgcttt
    12241 agtcttgtat gtctttgctc cttggctagc agacttgtct ggcgtgggca aagacttgat
    12301 cccaatcatg caaagcttgg cttggggagt cttgattttc ccgtctatga gtgttatccg
    12361 aggatttttc caagggatga ataacctcaa accctatgcc atgagccaaa ttgctgagca
    12421 ggtcattcgt gttatctgga tgctcctagc aacctttatc attatgaagc tcggttcagg
    12481 agattatcta gcagccgtta cccaatcaac ctttgctgcc tttgtcggta tggtagccag
    12541 ttttgcagtc ttgatttatt tccttgccca agaaggttca ctcaaaagag tctttgaaac
    12601 aggagataag attaacagta agcgtctctt ggttgatacc attaaggaag ccattccttt
    12661 tatcctgaca gggtctgcca tccagctctt ccagattttg gatcagctga cctttatcaa
    12721 tagtatgagc tggtttacca actacagcaa tgaggacttg gttgtcatgt tttcttattt
    12781 ctcagccaat cctaataaaa tcacgatgat tttgatttct gtaggggttt cgattgggag
    12841 tgttggtttg ccacttttga cggaaaacta tgtcaagggg gacttgaaag cagcttctcg
    12901 tctcgttcag gacagtctca ccctactctt tatgttcttg ctaccagcaa cggttggagt
    12961 ggttatggta ggagaacctc tttatacggt cttctatggt aagccagata gtttggctct
    13021 gggcttattt gtctttgcag ttttgcagtc tattatttta ggcttgtaca tggtcttgtc
    13081 tccaatgctt caggccatgt tccgcaaccg caaggccgtt ctctatttta tctatggttc
    13141 tattgccaag ctagtcttgc aactacctac catcgccctc ttccacagtt atggtccttt
    13201 gatttcaaca accattgctc tcatcattcc taacgtcttg atgtatcggg atatttgtaa
    13261 agtaactggt gtcaagcgca aggtgatttt gaagcgaacc attttaatca gtttgctgac
    13321 cctagtcatg tttctgttaa taggaaccat ccagtggctg ttaggatttt tcttccaacc
    13381 aagtggacgt ttgtggagct tcttttatgt agctcttgtc ggtgccatgg ggggtggact
    13441 ttatatggtt atgagtctgc gtacctattt attagataag gtaataggaa aagcccaagc
    13501 agatcgcctg cgagcaaaat ttaagctttc gtaaggtagc atttataaat aaaaggaata
    13561 agttgaacaa tcttttagga gaacttgaag cttattcctt ttttagcgta aaaatagcta
    13621 aaattatatt tcaaaaatag aaaatgaggc ttttttctat tttttggaaa aaatcattga
    13681 ctaaacaaac taaacgttgt ataataaaac aaatatttaa agcaggaggt tccggaaatg
    13741 aaaaagtcta agagcaaata tctaaccttg gcaggtcttg tcctgggtac aggagtttta
    13801 ttgagcgcgt gtggaaattc tagcacggcg tcaaaaacat acaactatgt ttattcaagt
    13861 gatccatcta gcttgaacta tctagcagaa aaccgcgcag caacatccga tattgttgca
    13921 aatttggtag acgggttatt agaaaatgac caatatggga atattattcc atcattagca
    13981 gaggattgga ctgtttctca ggacggtttg acctatacct acaaacttcg taaggatgcc
    14041 aagtggttta cttctgaggg agaagaatat gcgcctgtaa ctgcccagga ttttgtgaca
    14101 ggtttgcaat atgcagctga taaaaaatca gaagccttgt atctagtgca ggactctgtt
    14161 gctggtttgg atgactatat cactggtaaa acaagcgact tttcaactgt cggtgtcaag
    14221 gcacttgatg accaaacggt tcaatatact ttggttaaac cagaacttta ctggaattca
    14281 aaaacacttg caacgatact ttttcctgtt aatgcagatt tcctgaaatc aaaaggggat
    14341 gattttggga aggcggatcc atctagtatt ttgtacaatg gacctttctt gatgaaagca
    14401 cttgtctcaa aatctgctat tgaatataag aaaaacccta attactggga tgctaagaat
    14461 gtctttgtag acgatgtgaa attgacctac tatgatggta gcgaccaaga atcactggaa
    14521 cgtaatttta cagctggtgc ttatactacg gctcgtcttt ttcctaacag ctccagctat
    14581 gaagggatta aagaaaaata caaaaacaat atcatctata gtatgcaaaa ttcaacttca
    14641 tatttcttta attttaacct agataggaag tcttacaatt atacttctaa aacaagtgac
    14701 attgaaaaga aatcgactca ggaagcagtt ctcaataaaa acttccgtca ggctatcaat
    14761 tttgcttttg acagaacatc ttatggggct cagtctgaag ggaaagaagg tgcaacaaag
    14821 attttgcgta acctagtggt tcctccaaac tttgtcagta tcaagggaaa agactttggt
    14881 gaagttgtag cctctaagat ggtcaactat ggtaaggaat ggcaaggtat caactttgcg
    14941 gatggtcaag acccttacta caatcctgag aaagccaagg ctaagtttgc ggaagctaag
    15001 aaagaactcg aagcaaaggg tgttcaattc ccaatccact tggataagac tgtggaagta
    15061 acagataaag taggcataca aggagttagt tctatcaaac aatcaattga atctgtttta
    15121 ggttctgata atgtagtgat tgacattcag caattaacat cagatgagtt tgacagttca
    15181 ggctactttg ctcaaacagc tgctcagaaa gactatgatt tatatcatgg cggttgggga
    15241 cctgattatc aagacccgtc aacctatctc gatattttta atactaatag tggaggattt
    15301 ctgcaaaatc ttggactaga gcctggtgag gccaatgaca aggctaaggc agttggactg
    15361 gatgtctata ctcaaatgtt ggaagaagct aataaagagc aagatccagc caaacgttat
    15421 gagaaatatg ctgatattca agcttggttg attgatagtt ctttagttct tccaagtgtt
    15481 tcgcgtgggg gaacaccatc attgagaaga accgtaccat ttgctactgc ctatggttta
    15541 accggtacaa aaggggttga atcatataaa tacctcaaag tacaagataa gattgtcaca
    15601 acagacgaat atgcaaaagc cagagaaaaa tggttgaaag aaaaagaaga atccaataaa
    15661 aaagcccaag aagaattggc aaaacatgtc aaataaaatc taattgtaga taagtttgtg
    15721 taagaagtct ttgtgcttaa aaacgcataa tatcaggtat ataaaatcct agatgttatg
    15781 cgttttgtta taggaagatt tattagattt tatcctcaaa ttgagttatt cccgacttta
    15841 tccgttgaaa tattataagg agtatttatg aaattaaaat tattaagagt agatactaag
    15901 gtgattatgg ggagtttctt acttgttctg tctagtctac ttgctttgtt gcttcccctt
    15961 atcttaaagg gtttaataga tgggagttct attgaaaata taggttccaa agtatttcaa
    16021 tcgtttttga tttttattgg tcaagccttg ttttcttcta ttggttacta tctgtttagt
    16081 caatcgggtg aaaaaaagat agcaaaaatc aggaaaaaag tgatagaggg gttgatttat
    16141 gcagagaaat ccttctttga taagagccaa agtggggagt tgacttctgc cattgtcaat
    16201 gacacgagtg tcattcgtga gtttttaatt acgactttcc caaatattat tctgagttta
    16261 gttatggtac ttggttccat tgtagtctta tttagtcttg attggaatct ttctctactt
    16321 ttattcatca ctcttccttg tatgatgttt attatcttgc tcctttccaa tatcagtgaa
    16381 aagtatagtc gtcgtttaca ggaggaaatc ggatttttaa cagggcaatt gactgaaaag
    16441 attcaagaac atgaattaat taagactaac caagcagaga aaagtgtaca ggatgttttg
    16501 gataattgta ttgaaagggt acaaaacaat tcactgaaat cagatagggt tacttctttt
    16561 gagaccccgt ttgctctctt atttatcttt gcgactatag ctgtgatgct aacctatggg
    16621 ggttatcggg tcagcgcagg atatatatct gtgggaacct tggtttcgtt tttgatttac
    16681 ctctttcaat tacttaatcc tattagtaat atagctaatt ttgtaactgt ttattctagg
    16741 agcaagggat cttcagttgc actggagaac ttgcttgcag ttcctaaaga aaaatttgag
    16801 tgaggaaaat cggtatcagg acaagggttg aattttaacc atgtctattt tggttatgat
    16861 gaaaatcgac ctgtcttaaa ggatattact tgttcaattt tcaaggggca aaaaattgct
    16921 tttgttggac catctggatc aggaaaatca acgattgtgc gtttgttaga gcggttttat
    16981 aaaccgcttt caggagatat tctaatggag caatcaagta tatatgattt taacttaaaa
    17041 gaatggagaa gtaaaatcgc ttagtaactg atgatgaatt gatgaaagtg ctagacttag
    17101 tatcactagg tgatgagatt cgctccatga aagagggact agatactgaa gttggtgaac
    17161 gcggacgact cttgtcaggg gggcaaagcc aaagacttca aatagctaga gcctacttaa
    17221 aagatgctga aattcttata tttgatgaag ccactgctaa tcttgatgcg gattctgagt
    17281 atgcgattat cagtagcctc tattctgtat taaaggagaa gacggttgtg attatagcgc
    17341 atcgtttgtc aacggtaaaa gatgtggatt gtattttctt cttagaggag gggaaaatca
    17401 ctggctcagg aactcataag gaactactgg aaaatcatga gcgttatgct cgttttgtgc
    17461 aggagcaaat gatagagtga aggtgtcttt tgagattcac cattttatag tctattaaag
    17521 ggagcaggaa aaactccctt tttatatagt ttgaaactat aactagctct tgaaaagaag
    17581 aaaatgagtt gatgaaaata agtggtacaa tagttactat agatttggag gtattgtatg
    17641 agcaaggaat tacacattaa cacaattttg gcccaggcgg gtattaagtc agatgaagcg
    17701 acaggtgcat tggtgacacc gcttcatttt tcaacgacct atcagcatcc agagtttggt
    17761 cgatctactg ggtttgacta tacgcgcact aaaaatccaa ctcgtagtaa ggctgaggaa
    17821 gtcttggcgg ctattgagtc agcagactat gccctagcga ctagctcagg gatgtcagct
    17881 attgtactgg cctttagcgt ctttccagta ggaagtaagg tcttggcagt gcgtgatctt
    17941 tacggtggtt cttttcgctg gtttaaccaa gtggagcagg aaggtcgttt ccattttacc
    18001 tatgccaata cagaagaaga gttgattgcc gagttagaaa aggatgtgga tgttctctat
    18061 atcgaaaccc caaccaatcc cttgatgttg gaatttgata tcgaaaaact agcaaaattg
    18121 gctcatgcta agggtgccaa agtggtggtg gacaatacct tctatagccc tatctaccaa
    18181 cgtccgattg aagatggagc agatatcgtt ctccattcag caaccaagta tctagcaggc
    18241 cacaatgatg tcttggctgg agtggttgtg accaatagtt tagaactata cgagaagctt
    18301 ttttacaatc tcaatacaac aggggcagtc ttgtctccat ttgacagcta ccagttgctt
    18361 cgtggtctca agaccttatc tcttcgtatg gagcgctcaa cagctaacgc ccaagaagtg
    18421 gttgcctttt tgaaggattc tccagcagtt aaggaagttc tctacactgg tcgtggaggc
    18481 atgatttcct ttaaagtagc cgatgaaaca cgcattcctc atattttgaa cagtctcaag
    18541 gtcttctctt ttgcggaaag tttgggcgga gtggaaagtc ttattactta tccaacgact
    18601 caaactcatg ctgatattcc agcagaagta cgccattctt atggtttgac agatgacctc
    18661 ttgcgtttgt ctattgggat tgaggatgct agagatttga ttgcagattt gcgccaagcc
    18721 ttagaaggat aagacaaaga tgggaaaata tgattttaca agcctgccca accgtttagg
    18781 gcaccatacc tataaatgga aagaaacaga aacggatagt gaagttctac cagcttggat
    18841 agcggatatg gactttgtgg tcttgcctga aatccgccaa gccgtgcaaa cttacgcaga
    18901 ccaactggtt tatggttata cctatgccag tgaagactta attaaggaaa ttcaaaagtg
    18961 ggaagctaca caatacggtt acaactttga caaagaggct cttgtcttta tcgagggtgt
    19021 ggtaccagcc atctcaacag ctattcaaac ctttacaaaa gaaggcgagg cggttttaat
    19081 taacacgcct gtctacccac cctttgctcg cagtgtcaag ttgaataatc gtagattgat
    19141 tactaattcc ttagtggaaa aggatggtct gtttgagatt gactttgacc aacttgaaaa
    19201 ggatttggtg gaagaggagg ttaaactcta tattctttgc aaccctcaca atcctggtgg
    19261 acgtgtttgg gaaaaagaag tgttggagaa gattggccaa ctttgccaaa aacacggtgt
    19321 tttgttagtt tcggatgaga ttcaccaaga tttgaccctc tttggtcaca aacaccagtc
    19381 tttcaatacc atcaatcctg ccttcaaaaa ttttgctatc gtcttgagca gtgccactaa
    19441 aacatttaat attgctggaa caaaaaattc ctatgcagtc attgaaaatc ctaagttgag
    19501 actggctttc cagaaacgcc tgttggccaa taatcagcat gaaatttcag gcttgggtta
    19561 tttggcgaca gaagctgcct atcgatacgg taaagattgg ctagaggaac tcaagcaagt
    19621 ctttgaagac cacatcaatt atgtggtgga tctatttgga aaagagacta aaatcaaggt
    19681 catgaaaccg caaggtacct acttgatttg gcttgacttt tcagcctatg acctgactga
    19741 tgaaacattg caagagttgt tgagaaatga agccaaggtt atcctcaacc gtggtttgga
    19801 ttttggagag gaaggaagtc tccatgcccg catcaatata gctatgccca aatctctgtt
    19861 gcaagaagtc tgtcagcgga ttgtggctac ttttgccaaa cgttaaaaat ccagccttct
    19921 aggagaaaag tcttcctaga aggctatttt cataggcgaa aatatggtat aataaaaaga
    19981 taaggtaaag gtgaaaatat ggctaaattg attccgggga aaattcgtat cgaaggtgtt
    20041 gccctttatg aaactggtaa ggttgatatc atcaaggaaa agaacaatcg gctctacgct
    20101 cgcgttgcag aagaagaact gcgctatagt ttagaggatg atttggtttt ttgtgcctgt
    20161 gattcttttc aaaagagggg ctactgtgtg catttggcag cgctagagca ttttctgaaa
    20221 aatgatgagc gtggtcagga aatcttgtgg agtctggaag aaggtcatga agaaaaagag
    20281 gccgttgaaa ccaaggtgac cttgggtggc aagtttttga atcgaatttt atctccaaaa
    20341 tcagaatgcg cctatgagtt atcagctgtg ggtcaggtgg aagcaggaac caatcatatc
    20401 ttgtggactc tgagaatcgg ccaaatcaat agccaaaaat actatgtcat tcgggatatt
    20461 ccactttttt taaggattgt cgagcagaga aagtcgtata tgattggcaa gatttatgag
    20521 gagtctcttt cttgggaagc ctttgatgat gccagccaag aacttttgac tttcttgcac
    20581 ggactaacgg aggaagggct ggttccagac ctctttttcc aaaaccaagg tcggcacctc
    20641 tttttccctc tgaccttttt tgagcagggt gtgaatttac tgatggcctt gccccatttt
    20701 caatttgacc atcaagtgga tagctaccaa accctgcttt ttcaggatat gcatgctgat
    20761 gccaatctat ttgcctttac agtaacagaa tactcggact attttgaaat ggaaatcagt
    20821 gagagtccta gggtcaatgt cttttatcag ggagcagtac ttttccataa aggtcaggtt
    20881 tattttttaa cagaccagca gatgcgtctt ctcaaggaaa ttaaagcgtt acctgtgggt
    20941 caacaaggaa agaaatacct gcaatttgat agcagtgatc gagacaaatt ggcctcctgt
    21001 ctgactcttt ttatccagat gggcaccgtt tcagctccag aacgtctaca aatcaaaact
    21061 tttgcgccct ctttttactt tgacagggaa gaagataatc ggattcgttt agaaattcag
    21121 tttgattatg gaaatagaca ggtatctagc cgacaagagc tagaagaatt accattttca
    21181 agtgacgcgg acttagaaga aagaattttc caagtctgtt tggctgcggg ctttgaagcc
    21241 gattttcaat cttggcgtca ggccttaaaa gcggagtctg tctatcattt ttttcatgat
    21301 atcattccag tctttgaaaa actcgggcat gttgacctat cagataaact agaagagctt
    21361 tatagcctag cgagtcctca ggtgcagatt gcctccaagg gaggtctctt ggaaatccag
    21421 tttgattttc aaaatattgc ccaggaggaa atcgaccaag ccatgcaggc cttggttgcc
    21481 aatcaggatt tttatattga ttcgtctaat caagtttact ttttcgatga agaaaccaag
    21541 aaaattcgcc aaaatctaca ggaactgggg caatttgaat taaaagatgg gaccttgcag
    21601 gctcggaaat ccttggccta cagtttagct catctctttg aagggcgaga ccgtgtttct
    21661 ttttcacaag aattccagaa tctggcccag gatttgacgc atccagagga ctttcctttg
    21721 caagcaactc aggtcaaggc tgacttgcga gattatcagg aaaagggaat tggctggttg
    21781 cagatgctcc atcattatgg ttttggtggg attttggctg atgatatggg acttgggaaa
    21841 acccttcaga ccattgcttt tttgactagt caagtgacaa aagaaagtcg agtcttgatt
    21901 ctcgctccgt cgggtttgat ttacaactgg gcggatgagt ttcagaaatt tgccccacag
    21961 ttggatgtgg ctgttgttca tggtttgaaa gcaagtcgtg aggagattct tgccgagagc
    22021 catcaaatct atgtgactag ctatgccacc ttccgtcagg acagtgagtt ttatcagggg
    22081 atggcctttg acttcctttt cttagatgag gctcaggtca tgaaaaatgc ccagaccaag
    22141 attgcccaga ccttgagaca atttgtggtg ccgtcggtct ttgccttgtc aggaactccg
    22201 attgaaaacc atctgggtga gttgtggtct attttccaaa tcgtcatgcc cggacttttg
    22261 ccaagcaaga aagaatttat gaaattgcca gcagagcgag tggctcagtt tatcaagcct
    22321 ttcgtgatgc gacgcaagaa agaagaagtt ctgactgaat tgccagactt gattgaggtg
    22381 gtttataaaa atgaactgga agaccagcaa aaggctattt accttgccca gttacaacag
    22441 atgcgagacc atctggctca agtgtcagaa caggaatttc agcgaagtcg tgtggaaatt
    22501 ttatctggtt tgatgcgctt gcgtcaaata tgtgacactc ctgccctgtt tatggaagat
    22561 tatcagggag ccagcggcaa actagatagt ctccgagacc tactggtaca ggtggcagac
    22621 ggcggacacc gtgtcttgat tttctctcag ttcaagggaa tgttggaaaa aattgaacaa
    22681 gaactgccag acttgggcct gacttccttt aaaattacgg gttcaacccc agccaaggaa
    22741 agacaagaca tgaccaaggc ctttaatcaa ggagaaagag atgcctttct gatttccctc
    22801 aaggctggtg gggtcggtct gaacctgaca ggtgcagata cagtgatctt ggttgacctt
    22861 tggtggaatc ctgcggtgga agcgcaagcc attggccgtg cccatcggat gggtcaggaa
    22921 gaaacggttg aggtctatcg cttggtgacc aagggaacca ttgaagaaaa aattcaggaa
    22981 ctccaagaac aaaagaaaca tctggtgtca caagtattgg acggcacaga gtcacgtggt
    23041 agtctgaccc tagcagaaat tagagaaatt ttgggaattt ctgaagccag cacttgaaaa
    23101 atcaagacaa tacgctataa tgaagtgttg aaaggaaata gaaaatgaaa caagaacgat
    23161 ttccattggt gtcagatgac gaggtcatgt tgactgaaat gccagtcatg aatctctatg
    23221 atgagtctga tctgatcagt aatatcaagg gtgagtatcg agataaaaat tatttagaat
    23281 gggctcctat tgctgaagaa aaaccagtaa aaccgattga aaagcaagta gggaaaccta
    23341 aaaaagctcc tttaggggtt aaaaaagaag gaaagagcta tgcggaggtg gcgcgtgaag
    23401 aagcgcgtgc agacttgaaa aagaaacgct ctgctaacta tctaactcag gatttcagcc
    23461 ttgcgagacg tcattctcag cccagtctag ttagacaggg caatcaaccg acaactcctt
    23521 tccaaaagga aaatcctggt gaatttgtca aatatagcca aaaattgacc cagtctcatt
    23581 atatcttggc ggaagaagtt cattctatcc ctaccaagaa tgaagaagtg tcagcacctg
    23641 ctccaaagaa aaacaattat gattttctaa agaagagcca aatctacaat aaaaaaagta
    23701 aacaaacaga acaagaacgt cgggttgccc aagagttgaa tctgaccaga atgacagaat
    23761 agggagaaaa catgtcaaag acatatcatt ttatcggaat taagggatca gggatgagtg
    23821 ccttggcctt gatgttgcac cagatggggc acaaggttca gggatcagat gttgaaaagt
    23881 actactttac ccaacgcggt cttgagcagg caggaattac cattcttcct tttgatgaaa
    23941 agaatctaga cggtgatatg gaaattatcg ctggaaatgc ctttcgtcca gataacaacg
    24001 tcgaaattgc ctatgcggac caaaatggta tcagctacaa acgttaccat gagtttctag
    24061 gtagctttat gcgtgacttt gttagcatgg gagtagcagg agcacatgga aaaacttcaa
    24121 cgacaggtat gttgtctcat gttttgtctc acattacaga taccagcttc ttgattggag
    24181 atgggacagg tcgtggttcg gccaatgcca aatattttgt ctttgaatct gacgaatatg
    24241 agcgtcactt catgccttac cacccagaat actctattat caccaacatt gactttgacc
    24301 atccagatta tttcacaagt ctcgaggatg ttttcaatgc ctttaacgac tatgccaaac
    24361 aaatcaccaa gggtcttttt gtctatggtg aagatgctga attgcgtaag attacgtctg
    24421 atgcaccaat ttattattat ggttttgaag ctgaaggcaa tgactttgta gctagtgatc
    24481 ttcttcgttc aacaactggt tcaaccttca ccgttcattt ccgtggacaa aacttggggc
    24541 aattccacat tccaaccttt ggtcgtcaca atatcatgaa tgcgacagcc gttattggtc
    24601 ttctttacac agcaggattt gatttgaact tggtgcgtga gcacttgaaa acattttccg
    24661 gtgttaaacg tcgtttcact gagaaaattg tcaatgatac agtgattatc gatgactttg
    24721 cccatcatcc aacagaaatt attgcgacct tggatgcggc tcgtcagaaa tacccaagca
    24781 aggaaattgt agcagtcttt caaccgcata cctttacaag aaccattgcc ttgttggacg
    24841 actttgccca tgctttaaac caagcagatg ctgtttatct agcgcaaatt tatggctcgg
    24901 ctcgtgaagt agatcatggt gacgttaagg tagaagacct agccaacaaa atcaacaaaa
    24961 aacaccaagt gattactgtt gaaaatgttt ctccgctcct agaccatgac aatgctgttt
    25021 acgtctttat gggagcagga gacatccaaa cctatgaata ctcatttgag cgtctcttgt
    25081 ctaacttgac aagcaatgtt caataggatg ttcccatgga aattccaatt aagatcattc
    25141 aggcaagcaa gtctgatttg cctgagatag gggcacttca gacctcgtct tttccagctg
    25201 aaaagcagca actttcccat attttagaag aaagtatccg taagtgtgcg gatacctttc
    25261 ttctagctag ggatgaaaat caacttttag gctatatttt atcaagtccc cagtcagaca
    25321 atccgcaata tctaaaagta cattctttag tcatcgagtc tgaccatcag agacagggct
    25381 tgggaacact tcttcttgca gccttgaaag aggtggcagt tgatctggat tacaaaggga
    25441 ttcgtttgga gagtcctgat gagctgcttt cctattttga aatgaacggt tttgttgatg
    25501 aagaagcaac tttgctctat gcaactagcc agggctatag tatgatttgg tttaatccct
    25561 tttatctgga ggaacaatga aaatcagaca agcaagatta gaagatttgg atcggattgt
    25621 tgaacttgaa tttgaaaatt tttcggtcga agaagccatt cctccttctg tctttgaagc
    25681 acatttgaga gaaattcaga cctcttttct ggttgctgaa aaagaaggaa gaatcatggg
    25741 ttatatcgaa ggaccagttg gcctgcaccg ccatctgcaa gaccagtctt ttacagaaga
    25801 aataaaagac tatagtcatg agcctggtgg ttatatctct gtgacctgtc tgtctattgc
    25861 caaggaggca cagggactcg gactgggtca gaaattgctg acagctttga aagaagttgc
    25921 tcttgaagat gagagagatg gcattaatct aacctgtcat gactatctca tcgcctatta
    25981 tgaaaaacat ggatttgtca acgaaggcca gtcccagtca acctttgcag gggaaacatg
    26041 gtatgatatg gtctgggaaa tgaaaaaata agttaggaaa agtatcatag attgcttttt
    26101 ttggactttt aagatataat attatggatt atcaacaagg aggaaaaact tttgagtgaa
    26161 aagtcaagag aagaagagaa attaagcttt aaagagcaga ttctgagaga tttagaaaaa
    26221 gtaaaaggct atgatgaagt tctgaaagaa gatgaggcag tagttcgcac tcctgcaaat
    26281 gaaccttcag ctgaagaact catgactgat tccttgtcaa cggtagagga gattatgaga
    26341 aaagctccta ccgtgcctac tcacccaagt caaggtgtac cagcttctcc agcagatgag
    26401 attcaaagag aaactcctgg tgttccaagt catccaagtc aagatgtacc ttcttctcca
    26461 gcggaagaaa gtggatcaag accaggtcct gttagaccta agaaacttga aagagaatac
    26521 aatgaaaccc caacaagggt agctgtttcc tatacgacgg cagagaaaaa agcagaacaa
    26581 gcaggtccag aaacacctac gcctgctaca gaaacagtgg atatcatcag cgatacatca
    26641 cgtcgtagcc gtagagaagg agcaaaaccc gttaatccta agaaagagaa gaagtcacat
    26701 gtgaaagctt ttgtgatttc attccttgta ttccttgcct tgctctcagc aggtggttac
    26761 tttggttacc agtacgtgct ggattctttg ttacctatcg atgctaattc taagaaatat
    26821 gtgacggttg gaattccaga aggttcaaac gttcaagaaa tcggtacgac gcttgaaaaa
    26881 gctggtttgg taaagcatgg tctgattttt agtttttatg ccaagtataa aaattatacc
    26941 gacttgaaag caggttacta caatttgcaa aagagtatga gtacagaaga cttactcaaa
    27001 gagttgcaaa aaggtggaac agatgaaccg caagaacctg tacttgcgac tttgacaatt
    27061 ccagaaggtt ataccttgga tcagattgct caagctgtgg gtcaattgca aggtgacttc
    27121 aaagagtctt tgacagcgga ggctttcttg gctaaagttc aagatgagac gtttatcagt
    27181 caagcagtag cgaaatatcc tactttactg gaaagtttgc ctgtaaaaga cagcggtgcg
    27241 cgttatcgtt tggaaggata ccttttccca gctacatact ctatcaagga aagcacaact
    27301 attgagagct tgattgatga gatgttagct gctatggata agaacctatc tccttactat
    27361 agtactatca aatctaaaaa tttgactgtc aatgagttgt tgaccattgc ttccttggtc
    27421 gaaaaagaag gtgccaagac agaagatcgt aagctcattg caggtgtatt ctacaatcgt
    27481 ttaaatcgtg atatgccact tcaaagtaat attgcaatct tgtatgccca aggaaaactg
    27541 gggcaaaata tcagtctagc tgaggatgtt gcgattgata ccaacattga ttcaccttat
    27601 aatgtttata aaaatgtagg tctcatgcct ggtccagtcg atagtccaag tctggatgcg
    27661 attgagtcaa gcatcaatca aactaagagc gataacctct actttgtagc agatgtcaca
    27721 gaaggcaagg tctactatgc taacaatcaa gaagaccacg accgcaatgt cgctgaacat
    27781 gtcaacagca aattaaacta aacaaactaa aattatgtga tacgtcacat aattttcttt
    27841 agaaaatatt atcagaagaa agttgagaaa aatggcagaa aaaacatatc ctatgaccct
    27901 tgaggaaaag gaaaaacttg aaaaagaatt agaagaattg aaattggttc gtcgaccaga
    27961 agtggtagaa cgcattaaga ttgcccgttc atacggtgac ctttcagaaa acagtgagta
    28021 cgaagcagct aaggatgaac aagcctttgt cgaaggacaa atctctagct tagaaacaaa
    28081 aatccgctat gctgaaatcg tcaatagcga cgcagttgcc caggacgaag tagcgattgg
    28141 taaaacagtc accatccaag aaattggtga ggacgaagaa gaagtttata ttatcgtagg
    28201 ttcagctggt gcggatgcct ttgcaggtaa ggtttcaaat gaaagcccaa ttgggcaggc
    28261 cttgattggc aagaaaacag gtgacacagc aaccattgaa acgcctgttg gtagctatga
    28321 tgtaaaaatc ttgaaggttg aaaaaacagc ctaaaaacag aaaaaggagt ggggaggcga
    28381 tgtgcttcac tcactccttt ttccattttg ctattcttcg aaaatctctt caaaccacgt
    28441 cagcgtcgcc ttgccgtatg tatggttact gactttgtca gtttcatcta caacctcaaa
    28501 ac
//
LOCUS       DAVBCW010000017        27467 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVBCW010000017 DAVBCW010000000
VERSION     DAVBCW010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27467
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.017"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(69..365)
                     /locus_tag="VQ514_000621"
     CDS             complement(69..365)
                     /locus_tag="VQ514_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HET2499418.1"
                     /translation="MSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKY
                     PVVTDGVIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYE"
     gene            complement(518..974)
                     /locus_tag="VQ514_000622"
                     /pseudo
     CDS             complement(518..974)
                     /locus_tag="VQ514_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            1108..1935
                     /gene="xth"
                     /locus_tag="VQ514_000623"
     CDS             1108..1935
                     /gene="xth"
                     /locus_tag="VQ514_000623"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HET2499419.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            1947..2090
                     /locus_tag="VQ514_000624"
     CDS             1947..2090
                     /locus_tag="VQ514_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HET2499420.1"
                     /translation="MDYNAVIPEFLVSNIEQSRSFYCGLLGFRIEYQRSEENFLFLLK
                     SVN"
     gene            2090..2329
                     /locus_tag="VQ514_000625"
     CDS             2090..2329
                     /locus_tag="VQ514_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HET2499421.1"
                     /translation="MLEEGTKDQLAELTYPFGRGVNLSFGIKDVPKLYQKVMEANYPI
                     YRPLTKRKFRVSDPYIYPHKFAVLDPDGYFLRFSE"
     regulatory      2713..2808
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            3208..3789
                     /locus_tag="VQ514_000626"
     CDS             3208..3789
                     /locus_tag="VQ514_000626"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HET2499422.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAAAGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     gene            3789..5051
                     /locus_tag="VQ514_000627"
     CDS             3789..5051
                     /locus_tag="VQ514_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HET2499423.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LSAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(5138..6004)
                     /locus_tag="VQ514_000628"
     CDS             complement(5138..6004)
                     /locus_tag="VQ514_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HET2499424.1"
                     /translation="MKQTRNFDEWLSTMTDTVADWTYYTDFPKVYKNVSSIKVALNIM
                     NSLIGSKNIQEDFLDLYQNYPEILKVVPLLIAKRLRDTIIVKDPIKDFYFDFSKRNYS
                     IEEYTMFLEKSGIFDLLQNHLVSNLVDYVTGVEVGMDTNGRKNRTGDAMENIVQSYLE
                     AEGYILGENLFKEIEQNEIEEIFSVDLSAITNDGNTVKRFDFVIKNEQVLYLIEVNFY
                     SGSGSKLNETARSYKMIAEEIKAIPNVEFMWITDGQGWYKAKNNLRETFDILPFLYNI
                     NDLEHNILKNLK"
     gene            complement(5991..6761)
                     /locus_tag="VQ514_000629"
     CDS             complement(5991..6761)
                     /locus_tag="VQ514_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HET2499425.1"
                     /translation="MTKPYYNKNKMILVHSDTFKFLSKMKPESMDMIFADPPYFLSNG
                     GISNSGGQVVSVDKGDWDKISSFEEKHEFNRKWIRLAKEVLKPNGTVWISGSLHNIYS
                     VGMALEQEGFKILNNITWQKTNPAPNLSCRYFTHSTETILWARKNDKKARHYYNYDLM
                     KELNDGKQMKDVWTGSLTKKVEKWAGKHPTQKPEYLLERIILASTKEGDYILDPFVGS
                     GTTGVVAKRLGRRFIGIDAEKEYLKIARKRLEAENETN"
     gene            complement(6787..7641)
                     /locus_tag="VQ514_000630"
     CDS             complement(6787..7641)
                     /locus_tag="VQ514_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HET2499426.1"
                     /translation="MKIKEIKKVTLQPFTKWTGGKRQLLPVIRELMPKTYNRYFEPFV
                     GGGALFFDLAPKDAVINDFNAELINCYQQIKDNPQELIEILKVHQEYNSKEYYLDLRS
                     ADRDERIDMMSEVQRAARILYMLRVNFNGLYRVNSKNQFNVPYGRYKNPKIVDEELIS
                     AISVYLNNNQLEIKVGDFEKAIVDVRTGDFVYFDPPYIPLSETSAFTSYTHEGFSFAD
                     QVRLRDAFKRLSDTGAYVMLSNSSSALVEELYKDFNIHYVEATRTNGAKSSSRGKISE
                     IIVTNYEK"
     gene            complement(7798..8202)
                     /locus_tag="VQ514_000631"
     CDS             complement(7798..8202)
                     /locus_tag="VQ514_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET2499427.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERRVRI"
     gene            complement(8272..9753)
                     /locus_tag="VQ514_000632"
     CDS             complement(8272..9753)
                     /locus_tag="VQ514_000632"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HET2499428.1"
                     /translation="MTLVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLETIEDSQTAREILGTELMDLVTPCPSQVNRDFWEAYAYSPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIAVAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFARVLEDAGVYKQTEQGQTAFMRFVEHVGILLD"
     gene            complement(9772..10950)
                     /locus_tag="VQ514_000633"
     CDS             complement(9772..10950)
                     /locus_tag="VQ514_000633"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HET2499429.1"
                     /translation="MAQHLTTEALRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVFENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            11115..12122
                     /gene="galR"
                     /locus_tag="VQ514_000634"
     CDS             11115..12122
                     /gene="galR"
                     /locus_tag="VQ514_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HET2499430.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIQDKRLENFRNYSQAKGIYHDELVFQGN
                     FTAQSGYDLMKEAIQNLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHDRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            complement(12168..13205)
                     /locus_tag="VQ514_000635"
     CDS             complement(12168..13205)
                     /locus_tag="VQ514_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET2499431.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEVDDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATVVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(13206..13559)
                     /locus_tag="VQ514_000636"
     CDS             complement(13206..13559)
                     /locus_tag="VQ514_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET2499432.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            complement(13803..14693)
                     /locus_tag="VQ514_000637"
     CDS             complement(13803..14693)
                     /locus_tag="VQ514_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HET2499433.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            14826..15362
                     /locus_tag="VQ514_000638"
     CDS             14826..15362
                     /locus_tag="VQ514_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET2499434.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFQKNQDHITSLLFS
                     KNDYFLRQLQRELEHHVYPMLAADLKQTHPSLPTSYLQHLVVSNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            complement(15529..16335)
                     /locus_tag="VQ514_000639"
     CDS             complement(15529..16335)
                     /locus_tag="VQ514_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HET2499435.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(16680..18200)
                     /locus_tag="VQ514_000640"
     CDS             complement(16680..18200)
                     /locus_tag="VQ514_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HET2499436.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPSLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLAQKEKENLIIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            complement(18193..18921)
                     /locus_tag="VQ514_000641"
     CDS             complement(18193..18921)
                     /locus_tag="VQ514_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2499437.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKVPATDFVADLFGGSVHD"
     gene            complement(18936..19700)
                     /locus_tag="VQ514_000642"
     CDS             complement(18936..19700)
                     /locus_tag="VQ514_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET2499438.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFREDENG
                     CIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHEIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            complement(19704..20147)
                     /locus_tag="VQ514_000643"
     CDS             complement(19704..20147)
                     /locus_tag="VQ514_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET2499439.1"
                     /translation="MKDSHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKFQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            complement(20829..21125)
                     /locus_tag="VQ514_000644"
     CDS             complement(20829..21125)
                     /locus_tag="VQ514_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HET2499440.1"
                     /translation="MAEPNLESLIKDLYNHARHDLSEDLVAALLETAKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLARFIKREEAKYRGTATSALMYEELFKML"
     gene            complement(21147..22211)
                     /gene="pepA"
                     /locus_tag="VQ514_000645"
     CDS             complement(21147..22211)
                     /gene="pepA"
                     /locus_tag="VQ514_000645"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HET2499441.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            22612..23313
                     /gene="galE"
                     /locus_tag="VQ514_000646"
     CDS             22612..23313
                     /gene="galE"
                     /locus_tag="VQ514_000646"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET2499442.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYKVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            23736..24695
                     /locus_tag="VQ514_000647"
     CDS             23736..24695
                     /locus_tag="VQ514_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2499443.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKEISESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLGFILLLWRGGRKDAD"
     gene            24685..25641
                     /locus_tag="VQ514_000648"
     CDS             24685..25641
                     /locus_tag="VQ514_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HET2499444.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLTLQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRSRQ"
     gene            25638..26390
                     /locus_tag="VQ514_000649"
     CDS             25638..26390
                     /locus_tag="VQ514_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2499445.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGS
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            26486..27451
                     /locus_tag="VQ514_000650"
     CDS             26486..27451
                     /locus_tag="VQ514_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2499446.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPAILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
BASE COUNT         8102 a         6335 c         4890 g         8140 t
ORIGIN      
        1 acatacggca aggcgacgtt gacgcggttt gaagagattt tcgaagagta caagacccca
       61 ggtttttctt attcataagt tacgactgta acagcaccct tgtcatattc agcaataaag
      121 atattggcta cattgtcatg cccttgtttg ctgaggttat caagcaacca ctcttcgcta
      181 cgaccaatcg attccaagac gtctacttga atcacaccgt cagtcacaac tggatactta
      241 ggattttcat ctcccatttg cacaacgatg agttgcccat tttgctcttg cacagctcgt
      301 ttgacttgct tcatctggaa aatcccttgg ctacgaagtt tgagggctac atccgatgca
      361 gacaaaccaa ctgaacgaca ggcttatggg tcaatattcc catttttgat aaggagagtt
      421 ggcttaccat caatcaaacg tttcacaaag tgaacattgt tgttgagcta tttgagagtc
      481 aagatcagtt tgattaaaat ttcgatataa ttaagtgtca tttgttcaca tccaccagtt
      541 caattgcgtc tgtcttatac aattcgactt tctctaacag gtacttgtct ggctcacttc
      601 cgtttagggc acgataatag cgatctccca ccttgataag tgcgccatct gtgtttgtgg
      661 aagtattaac atggacttct gacttgtcta ctcccaaatc tttggaaaca acctcgatga
      721 aatgaagtga agtttgaaat ttattgttag aaacttgatt gttttgataa gtcgtgatgc
      781 tgaccaaaag catagctaaa taaggccaga gctgaaatca tgaccaactc acgaaatttg
      841 gttccttttt tatcatggta tgccttgaaa gcaaaaaaaa ctgtcacagc taatagaaca
      901 atcccaaagc caaccatgat accattttgc tgaccgattt ggctgagtac atagtcataa
      961 gagtagaatt tcatgtattt tcactccctt tcataaaatc attcttaatt ataaacgaaa
     1021 gagagtaatt tttcaaacca tacgttgggg aaatagatct ttttttggta taataaaatc
     1081 tataatctga atgaaaaagg taactttatg aaacttatct catggaatat tgattcccta
     1141 aacgctgccc taactagtga ctcagctcgt gccaaattgt cccaagaagt cctacaaacc
     1201 ttggtcgctg aaaatgctga tatcattgct atccaagaaa ccaagctttc tgccaaagga
     1261 cctacaaaga aacacgtgga aattttagaa gaactcttcc caggctacga aaacacgtgg
     1321 cgctcttccc aagagcctgc ccgtaaaggc tatgctggaa ccatgttcct ttataagaaa
     1381 gaacttacac ccactatcag cttcccagaa atcggtgccc cttctaccat ggacttggaa
     1441 ggccgtatca tcactctaga atttgatgca tttttcgtaa cccaagttta cactccaaac
     1501 gctggtgacg gtctcaaacg cttggaagaa cgccaagtct gggatgtcaa atatgcggag
     1561 tatttggctg aactagacaa agaaaaacca gtccttgcga ccggtgacta caacgtagcc
     1621 cacaatgaaa tcgaccttgc aaatcctgct agcaaccgcc gttcacctgg atttactgac
     1681 gaggaacgtg ctggatttac caacctattg gcaactggat ttactgatac cttccgccat
     1741 gttcatggcg atgttcctga acgctacact tggtgggcac aacgcagcaa aacttctaaa
     1801 atcaacaata caggctggag aatcgactac tggctcacaa gtaaccgtat cgctgacaag
     1861 gtcactaaat ctgatatgat tgactcaggt gctcgccaag accatacacc gattgtcttg
     1921 gaaattgact tgtaaggatt ttctatatgg actacaatgc ggtcattccc gagtttcttg
     1981 tatcgaatat cgaacagtcc cggtccttct actgtggttt actgggtttt aggattgaat
     2041 accaacgttc agaagaaaac tttctctttc tcttgaagag tgtcaactaa tgttagagga
     2101 gggaacaaag gatcaactag ctgaactgac ctaccccttt ggtcgtggag tcaatctctc
     2161 ctttggcata aaggatgttc caaaactcta tcaaaaagtg atggaggcaa actatcctat
     2221 ttatcgtccc ttgaccaaaa gaaagtttcg tgtcagtgat ccctatatct atcctcataa
     2281 atttgcagtt ttggatccag atggctattt tttaagattt agcgagtaga ataataaaaa
     2341 ttgggaaaag tctttaaaag ttagaaaaag gcatattgtc aggtgtctaa aaacttgata
     2401 gtatgccttt tatgatggaa aaatcttcta atactcttcg aaaatctctt caagccacgt
     2461 caacgtcgcc ttaccgtagg tatataatac tgactctgtc aattctatcc acaacctcaa
     2521 aacagtgttt tgaggttgtg gatagcttcc tagtttgctc tttgattttc atttagtata
     2581 aatgttagct tacaatggag tttttgttca ccctctcttc tgtttgttcg ttttttaaac
     2641 caaaacacta acaaaatagt tgatttctaa agttatttag tgtaaaataa aaacattggc
     2701 tcaagcctat ttaaaattga atatcgtttt acttgtttat gtcgtgaatt ggcacgacgt
     2761 ttctacaagg tgccggaaca cctaacaata agtaagtcag cagtgagata tggccgtttt
     2821 tgccatgcct atttggggga cttacttaga gattaagtag gtcttttttg aatattttta
     2881 atatttccct taattagtgc ggacgggagg tgaaattatc ctgtgaataa tttcaggaat
     2941 ccagggaatt tcatccctaa tttttgagcc cctagctctt tcatttctag gcttaggcta
     3001 aaatagttcc ccgaactatt ttactctcaa aaatcccgtc atagacaata acaagattat
     3061 tgtctatgat accactacaa cgggaaatat ttgataagac tcgcttcgct cgtaatagtt
     3121 ttatcaaata tatagcatta gcaaaaggga tcactataga aacatttaaa caacaagtaa
     3181 attaactttt agactttagg aggtcttatg aaattattag aagagcgcat cctcaaggat
     3241 gggcatatct tgggtgataa catcctcaag gtagattcct ttttaaccca ccaagttgac
     3301 tttagcttga tgcgagagat tggtaaggtt tttgcggaaa aatttgctgc tgccggcatt
     3361 accaaggtcg taaccattga agcgtcgggt attgccccag ccgtttttac agctgaagcc
     3421 ttaaacgttc ccatgatttt cgccaaaaaa gctaagaaca tcaccatgaa cgaaggcatc
     3481 ttaactgctc aagtctactc ctttaccaag caggtgacca gcaccgtttc tatcgctgga
     3541 aaattcctct caccagagga caaggttttg attatcgacg atttccttgc taatggccaa
     3601 gctgctaaag gcttgattca aatcatcgaa caggccggtg ccacagtcca agctatcggt
     3661 atcgtgattg agaaatcctt ccaagatggc cgtgatttgc ttgaaaaagc aggctaccct
     3721 gtcctatcac ttgctcgttt ggatcgtttt gaaaatggtc aggtcgtatt taaggaggca
     3781 gatctctaat gcaaactcaa gaaaaacact cgcaagcagc cgttcttggc ttgcagcact
     3841 tactagccat gtactcagga tctatcctgg ttcccatcat gattgcgaca gcccttggct
     3901 attcagctga gcagttgacc tacctgattt ctacagatat cttcatgtgt ggggtggcaa
     3961 ccttcctcca actccaactc aacaaatact ttgggattgg actcccagtc gttcttggag
     4021 ttgcattcca gtcggtcgct cccttgatta tgattgggca aagccatggt agtggcgcta
     4081 tgtttggtgc ccttatcgca tctgggattt acgtggttct tgtttcaggc atcttctcaa
     4141 aagtggccaa tctcttcccc tctatcgtaa caggatctgt tattaccacg attggtttaa
     4201 ccttgatccc tgtcgctatt ggaaatatgg gaaataacgt tccagagcca actggtcaaa
     4261 gtctcttgct ttcagctatt actgttctga ttatcctctt gatcaacatc tttaccaaag
     4321 gatttatcaa gtctatctct attttgattg gtctggttgt tggaactgcc attgctgcta
     4381 ctatgggctt ggtggacttc tctcctgttg cggtagctcc acttgtccat gtcccaactc
     4441 cactctactt tgggatgcca acctttgaaa tctcatctat tgtcatgatg tgtatcatcg
     4501 caacggtgtc tatggttgag tcaactggtg tttatctagc cttgtctgat atcacaaaag
     4561 atccaatcga cagcacgcgc cttcgcaacg gttaccgcgc agaaggtttg gccgtacttc
     4621 tcggaggaat ctttaacacc ttcccttaca ccggattttc acaaaacgtt ggtttggtta
     4681 aattgtcagg catcaaaaaa cgcctgccaa tctactacgc agctggtttc ctggttctcc
     4741 ttggactgct tcctaagttt ggtgcccttg cccaaatcat tccaagctcc gtcctcggcg
     4801 gtgccatgct ggtgatgttt ggttttgtat ctattcaagg gatgcaaatc ctagcccgag
     4861 ttgactttgc taacaatgaa cacaacttcc ttatcgcagc tgtttcaatc gctgcaggtg
     4921 tcggtctcaa caacagtaat ctctttgtca gcatgccgac agccttccaa atgttcttct
     4981 caaacggaat cgtcgtagcc agcctactcg ccattgtcct caatgccgta ttaaatcata
     5041 aaaagaaata agaaaaagag gtgcgagcct cttttttgtt tatcacaaat tgattataaa
     5101 tacaatcaat cccaattccc atctaattga attggaccta tttcaaattc tttaaaatat
     5161 tatgttccaa atcgttaata ttatataaga atggtaaaat atcaaatgtt tctcgtaaat
     5221 tattcttagc cttataccaa ccttgtccat cagtaatcca cataaactca acattaggaa
     5281 tagctttaat ttcttcagca atcattttgt aagatctagc cgtctcattt aattttgaac
     5341 cacttcctga ataaaaatta acctcaatca gataaagcac ttgttcattt tttatgacaa
     5401 aatcaaaacg tttcacagta ttcccatcat tcgtaatagc agataaatcg acagaaaaaa
     5461 tttcctctat ttcattttgt tcaatctctt taaataaatt ctctcctaaa atataccctt
     5521 cagcttcaag ataactttga acaatatttt ccatcgcatc tccagtccga tttttgcgcc
     5581 cattagtatc cataccaacc tcaacacctg taacgtagtc aactaaattt gatactaaat
     5641 gattttgcaa taaatcaaat atacctgatt tttccaaaaa catagtatat tcttcaatac
     5701 tatagtttcg tttactaaaa tcaaaataga aatcttttat cggatcctta acaataatcg
     5761 tatctctcaa acgtttagca ataagtagag gcacaacttt caaaatttct ggataatttt
     5821 gatataagtc taaaaaatcc tcttgtatat ttttactacc aataagacta ttcataatat
     5881 ttagagcaac ttttatacta gatacatttt tataaacctt gggaaaatct gtataataag
     5941 tccagtcagc tactgtgtcg gtcatcgttg acaaccactc atcaaaattt ctagtttgtt
     6001 tcattttctg cctccaacct cttccttgca atttttaaat attctttttc agcatcgata
     6061 cctataaatc ttctacctaa ccgcttcgca acaacacccg tagtgccact accaacaaat
     6121 gggtctagaa tatagtcacc ctctttagta gaggctaaaa taatgcgttc taacaaatac
     6181 tctggttttt gagttggatg tttcccagcc catttttcaa ctttctttgt taaagaaccc
     6241 gtccagacat ctttcatttg ttttccatca ttcaattctt tcattaaatc ataattgtag
     6301 taatgacgag cttttttatc attttttctg gcccataaaa tggtttcagt agaatgggta
     6361 aaataacgac aagataaatt gggggcaggg tttgttttct gccaagtaat attattcaga
     6421 attttaaaac cttcttgttc taatgccatt ccaactgagt atatgttgtg caaactacct
     6481 gaaatccata ccgtcccatt aggcttcaga acttcttttg ctaggcgaat ccatttacga
     6541 ttaaactcat gtttttcttc gaatgaagaa attttatccc aatctccttt atcaacagaa
     6601 acaacttgtc ccccagaatt agatattcca ccattactta aaaaataagg tggatcagca
     6661 aaaatcatat ccatactttc tggtttcatt tttgataaga acttgaacgt atctgaatga
     6721 acaagaatca tcttattttt attgtagtat ggttttgtca taagaacacc tccatactta
     6781 tattcgttat ttttcataat ttgtgactat aatttcagaa atttttcctc gacttgaaga
     6841 ttttgctcca ttagttcggg tagcttcaac ataatgtata ttaaaatcct tatacaactc
     6901 ctctactaaa gcactagaag aatttgataa cataacataa gctcctgtat cactcaatct
     6961 cttaaaggca tctcttaatc ttacttgatc tgcaaaagag aatccctcat gagtataaga
     7021 cgtaaatgca ctcgtctcag acaatggaat atatggaggg tcaaaataca caaaatctcc
     7081 tgttcgaaca tctacaattg ccttttcaaa atctcccact ttaatttcta gttgattgtt
     7141 atttagataa actgaaatag cagatatcaa ttcctcatca acaattttag gattcttata
     7201 acgtccatat ggaacattaa attggttctt agaattcaca cgatatagac cattaaagtt
     7261 cactctcaac atatatagaa tacgtgcagc tctttgtact tcggacatca tatctattct
     7321 ttcatcacga tctgcagaac gtaaatctaa ataatattct tttgaattat attcctgatg
     7381 aactttcaaa atttcaatca attcttgagg attgtcctta atttgttgat agcaatttat
     7441 tagttcagcg ttaaaatcat taataactgc atctttagga gccaaatcaa aaaataaagc
     7501 tccacctcca acaaaaggtt cgaaatacct gttataggtt ttaggcatta attctctaat
     7561 aacaggcaat aattgtcttt taccacctgt ccatttcgtg aacggttgta aagtaacttt
     7621 ctttatttct tttatcttca tgcttttaat tataccatag aaattaaaac acaactcttg
     7681 tattttctac cacattacta aattttctat tttagaatta aatcatatca atataaaaaa
     7741 atgagtaagg caaatcttat aaattccaaa attcacctca ctctattaaa ttactcttta
     7801 tatccttacc cgtctttcct cagacctttg gcctgtgaca aacatagtaa aggttgcttt
     7861 gcagacattt ctgccctctt gattggtgat atccacatcc atcacacagg ttgtacgacc
     7921 ttgatggaca cattctcctt taatggtcaa tacatcgtcg agttttcctg ccttaaggta
     7981 gttgatagag gattggagtg tcaccccatc aagtcccagc gagataacca ccaaaccact
     8041 gatttggtca catagtgtga atagatagcc accatgggca ttgccatagt agttgagcga
     8101 cgagttcact actttggtcg tcaccacaac gtgaccatct ctcatttttt caatttcgta
     8161 attttcaaag gcagatatag cgtcaaaatg aaagtctttc atcgtttcct cctgatattt
     8221 caaatgacac tatgatacta ctttttatag gactgtgcaa ggagaaagct cctagtctag
     8281 taaaattccg acatgttcca caaagcgcat aaaggctgtc tgcccttgtt ctgtctgctt
     8341 gtagactcct gcatcctcaa gtacacgcgc aaagatagca cccacagagt ccttgacgat
     8401 tgcaagggct ttttctttat ccgttaggtc tggatgttgg gatttgagtt ggtctgccca
     8461 ctcctgatga taatcggcaa ctgtaacagc ttctcctaca agatagctag cgacttgctc
     8521 tacttcttct ttcagacgtg gtggcaagat tgccaagccc atgacctcaa tcaagccgat
     8581 attttccttc ttgatatgtt ggacatcctt gtggggatga tagatgccat caggatgctc
     8641 tgctgaagtc tgattgtctc gcaagaccaa gtccaactca aactgtccat cacgcttacg
     8701 cgctattggt gtgatggtgt gatgcggtgt cccgtctgtc tctgctaaaa tctgcacact
     8761 agaatctgaa tactgacgcc attcctggaa aatcttatca gctaagttaa tcaaatcctc
     8821 tttggaatcc gaagtcaagc gtaggacaga cattggccac ttgataattc cagccttgac
     8881 ctgctcaaaa ccagcaaatc ggaaggtctt ttgcaaggga gccaattcca taggaaatac
     8941 gtgacggcct ccctgataat gatcatgagt tagaatagag ccccccacaa tcggcaggtc
     9001 ggcattagat ccagcaaaat atcctggaaa ctgctctacg atagccaaca gacgttcaaa
     9061 actctgacga ctaatggcca tgggacgatg ctggccatct aaaaagatac aatgctcatt
     9121 aaagtaagca tagggcgaat actggaaacc ccattcctga ccaaccattt caaaacggat
     9181 aatccggtgg ttgctgcgag ctgggtggtt gacccgacca tggtaaccct cattctctaa
     9241 acaaagttga cactgaggat aattactagc ttgcaccaac ttggctaccg caatctcttt
     9301 cggatccttt tcaggcttag agagattgat ggtaatttca agttctccat aatcagatgg
     9361 aacacgataa gcgatatttt tagcaatggc cttgagtttg atgtagtcat ttttctgact
     9421 gagttgataa aaatcctcta tcgcttgctc aggagagtag gcataggctt cccagaagtc
     9481 acgattgacc tgactcggac aaggagtcac caagtccatc aattcagtac caaggatttc
     9541 acgcgcagtc tgactatcct caatcgtctc taatcgaacg gcttcctcaa ctagctggtc
     9601 cttgaggtca atcaatttat ccagattggt ctcaacttcc aaaacacctt ctcccactcg
     9661 tgccaaaaca cgattggtca ggtagattcg atccatttcc tcaaatgaac tttcagaaat
     9721 gacatgtgtt acaaatttat ctactaaggt cactatagag cctccttttg actagtcaag
     9781 gacgcgagtg ccacctgcaa cttcagcgat atagaagctt ggagcgtatc caactacttc
     9841 ctcgtagtgt ttgcctacag cttccttaaa ggcctcaaca gtatcttttt gaaccaaggc
     9901 aatggcacag ccaccaaaac cagcccctgt catacgagca ccgagaactc cttcttgtgc
     9961 ccaagctgtg tgaacaagag tatccaattc caaaccagtt acttcataat catgctccag
    10021 agaaacgtgt gacgcattca tcaaacgacc aaatgtttcc aaatcacctg cttgaagggc
    10081 tgcttgagct ttaagggtac gttggttttc aaacacagca tggcgagcac gtttcaaacg
    10141 attttcatct ttaatcagat agctatattg gtcaacggcc cactcgtcca attcacccag
    10201 ggtctgaata tccaaggcaa cttgcaattc ttccactgct ttttcacact cagcacgacg
    10261 ttcattgtat ttagagtccg ccaattcacg gcgtttgttg gtgttcatga taacaacgac
    10321 attgtccttc aaatcaagtg gcaccaagtc gtattctaaa gtattggtat ctaagtaaat
    10381 agcacgttgg tcagccccca taccaatagc aaattggtcc atgataccag agttgactcc
    10441 gataaagtta ttttctgttt gttttccgat tttaaccaaa tcgagacgct ctagttttaa
    10501 atcaaagaga tgctctgcca cgactcctgt caagagctcc aaggatgctg aagaagacaa
    10561 accagcacca tttgggatat tcccataaac ataaaaatca aaacctttgt caatcacgtg
    10621 cccagcttct tgcaagaaat gaaggacacc ttttggataa ttggtccagt tgtgctcttt
    10681 ttcaaacttg aggtcagcga gaggcacttc gataatgccc ttgtcctcaa agttagctga
    10741 gtagaaacgc aagacttggt cgtcacgctt acgagctgca ccgtaagttc ccaaggaaat
    10801 agcagcagga aaaacgtgcc caccgttgta gtctgtgtgt tcaccaatca aattaatgcg
    10861 gcctggtgaa aagaaggttt ggtctgcttc ttgaccaaaa acagcaagaa agtctttgcg
    10921 aagggcttca gtagtaagat gttgtgccat atgaattctc ctttgactgt ccgttaagcc
    10981 accttaacgt ataatcgttt tctttctatt gtatccaagg aattttccaa gatcaatcct
    11041 ttttactaaa attttactaa aatatcagta aaaagcagaa aaatatgata tactaaccta
    11101 atcaaggagg atttatggct accttaaaag acattgcaca gctagcctct gtctctatcg
    11161 cgaccgtatc ccgcgtcctc aatcgcgacc agagcctatc tgttacagaa gaaaccagac
    11221 accgtatttt aaccgttgct gaagagctgg gctacaccaa gcacctcaag acaggcgagt
    11281 cccacaaacc caagcaaaag attgccatta tccaatgggt cagcgaacaa ggggagctgg
    11341 acgacctcta ctactaccag attcgcctag gaatagaaaa aagagcccaa gagttggact
    11401 atgatatctt gcgctatttt aatgaccatc cttttacctt aagcgaggaa gtgattggga
    11461 ttctctgcat tggaaaattt agccgagctc agatttctgc ctttgaagaa taccaaaaac
    11521 ccctggtctt tctagacagt gatacacttt ctctggggca tacttgtatt attacggatt
    11581 tttacactgc catgaaacag gttgtcgatt atttcctcag ccaaggaatg gaccgtatcg
    11641 ggattctaac aggccttgaa gaaacaactg accaagaaga aatcattcag gataagcggc
    11701 tggaaaattt cagaaactac agtcaagcaa aaggaatcta tcatgatgaa ctggtctttc
    11761 aaggaaactt tactgcccag tctggctatg acttgatgaa ggaagccatt cagaacttgg
    11821 gagaccaact cccaccagca tttttcgcag ccagcgatag tttagctatc ggtgcccttc
    11881 gtgccctcca agaagctgga atcagcctac cagaccgtgt cagcctcatt tcctttaacg
    11941 acaccagcct gaccaagcag gtttatcctc ccctctccag catcacagtt tatactgaag
    12001 aaatggggcg agcaggtatg gatattctta acaaggaagt cctccacgat cgcaaaattc
    12061 ctagcctgac catgctggga accagactga cattaagaga aagtacccta aatcaagaat
    12121 aggataacat aaaaaacgaa tagagttcta aaactcctat tcgtttttta ttcgattaca
    12181 atcatagact taatggtctt acgttcatcc atatctttgt aggcttggtc gatatcttcc
    12241 agtttataac ttgaagtaaa gacgcgacct ggattgatat caccatcaag gacggctttt
    12301 agtaaaaatt gcttatcgta tgttgtagca gaagctgccc cacctgctac agagatattt
    12361 tgcataaatg tcgaaccaag agcacgatta ttatagtgtg ggactcctac aaagcccata
    12421 cgccctccat tatgaagaac acctagcgcc tgttctatag cagcctccgt accaacacat
    12481 tcaagtgctg cgtctgctcc tccaccgagg atttcacgca ccttggtaat tccttcttga
    12541 ccacgttctg caacaacaac tgtcgcacct gactccatag ccatcttttg acggtcttca
    12601 tgacggctca taaggataat ttgtgatgct ccacgcatct tagccgcgat gacagcacat
    12661 tgaccaacag ccccatcacc gataacaaca accttgtccc ctttttgaac atttgcaaca
    12721 cgcgccgcat gatagcctgt cggcatgaca tctgcaagag tcaaaaggga cttgagcatc
    12781 ccttctgtat agtcagaagg ttgaccaggg attttaacca gcgcccagtt tgcatagtgg
    12841 aagcgaatat attctgcctg aaaatcaccc cccaaattat tgccaatatg attgtcgcaa
    12901 gaaccgtcaa atccagcaag acaggcatca cactcaccac atccatgtgt aaaagggaca
    12961 atcacaaaat cacctggttt caccgtcgta atggcttccc cagcttcttc aacaatccca
    13021 atcgcttcgt gtccactatt tttgtgtcca gctttcgttt ctggattacg gtacctccat
    13081 aaatctgaac cacaaacgca cgcacgaacc acacgaataa tcacatcatc cacttctatt
    13141 atttgcggac gttcaatgct agcaagtcca acctgacctg cctttgtata tactgctgat
    13201 ttcatttaaa attttccttc cttataaagt ttaattttga gatttaaacg atttaaagct
    13261 gtctgtagct gagacaagcg ctcctctaat ttttgctttt cctcttctaa aataccaagc
    13321 ctctcctctc tcgtttcatc tcccttttgg tagagcgaca tatagtcaac taaactatct
    13381 acagatacac ccgccgaacg aaaacactta ataaattcca gcgcttcgat atcctgatct
    13441 tgaaaatcac gaatcccagt agcagtacga gtaatcggtg gcacaagacc aacccgttca
    13501 taataccgaa tcgtatccgc tgaaattccc aacaaatcac tggcagattt aatattcaca
    13561 cgctaacctc ctctcatttc attcactgaa ttcacgctat tctaactcac ttatcttatt
    13621 atataacttt gagttgactc caagtcaagc attttgtatc taatcgaaaa atttttatca
    13681 ttaacaatca tatatccgga gtttatacaa tgctcttctc ttaccaattt ttgaaatatt
    13741 taaataataa agaaattact ctattccaaa taagatagta cccaagtatt tttcactttt
    13801 ttctaatgtt gatgctcata actccgttcc aagtcacaca cctttcgctt atgggtttgg
    13861 tgagtttcta ggtcagcatc aatttcaatg gtaatatttt gaaaaccaca atcttttagg
    13921 aaaattcgaa tagactcttt acaagtttcc atatgttcca tttcttttag acaaacatgg
    13981 acaatggcat ttttttccaa agcatccata gtccagagat taagctgatt aaggctggcc
    14041 acattgtcca atcgctccag gccactcttt acttgcttga tatcaagacc ttctggcaca
    14101 gcatccaaga aaatcttgag tgtagaccaa aaacgtggaa gggcttttga aagaataaag
    14161 aaagaaatga caagggacaa aagaggatct aggatatacc agtccgtaaa tcgaagaaca
    14221 atcgccatca ggataacagc tacccaccct agcgtatctt ccagaaaatg cagactcaga
    14281 atagactcat tctttgtctt tcccttacca accaccagac tcgctaacag attgatagta
    14341 atcgcaataa ttcctaacca gagaatcccc tcatcattga ctggttgcgg atgcaaaatc
    14401 ttcgtgacat tttccaaaat gactagaaca gagcccgtta cgagaatcac agctgttacc
    14461 aaggctccta gcaggctaaa ccgcttatag cccaaggtgt actgattgtc ttcttcacga
    14521 ttggagattg tttctagaaa agctgatatt ccaattgcaa tcgcatctcc caagtcatgc
    14581 acagagtcag caagaacagc gctagaacca aatactccac ctgcaataaa ctcaacaatg
    14641 gcataagtca aatttaagaa aaaagccacc caaacagcat attttgcctt catatttctc
    14701 attcctttgt tataatagat ttatgaacac cttgttcatt atcattatcc actaaaactc
    14761 aaagaaagga tagaagcaaa acgtcagctt tattcagttc tgaacaattt gcctcaagtg
    14821 tccatatgac taacattgac cgccgtatca gcaaaaccaa aaaagccatc tatcaagctt
    14881 ttatacaact tttgaatgct aagggctacg aggccactac tgttcaggat atcattgatc
    14941 tcgcagatgt gggacgatcc accttttact gtcactatga gagtaaggag cttcttctgg
    15001 accagctctg ccgctacctc ttccatcatc tttttgaaag agagcaagcc atttcaaccg
    15061 aagattacct agcccacctc tttctgcatt ttcagaaaaa ccaagaccat atcaccagtc
    15121 tactattctc caaaaatgac tatttcctcc gtcaactaca gagagagctt gagcaccacg
    15181 tctatcccat gctggctgct gatttgaagc aaactcaccc aagtctacct acttcttacc
    15241 tccaacactt ggtcgtgtcc aactttatcg agacattgac ctggtggctc aaaaaaggtc
    15301 aagatttcac agaccaggaa gttgtccaat tttatctaga ccttctcatt cctaaaaatt
    15361 gaatatagag taaagcttca gttgtcttat ttctaggtta ctgagttttt tatcttttca
    15421 acaacaaaag aggacccgcc gatcctcttt ttcatactat aaatccttga ttatcaatta
    15481 tatctgtttt aatcgaaatc tcaaaacagc actttcaaac atcttttcct agttaagtaa
    15541 atcagtattc tgcttagctg ccttgctcca ttgataccaa ccaactagac tgttaatgag
    15601 ataaattaga tatttccctt gaatttgcag gctttctccc caccagagat agattgaaaa
    15661 gacattggta gccgcccaga atatccactg ttcacggtaa acagctgtca tgaggatttg
    15721 ccctacccca ttggttgcat ctgtgattga atcacgatag ggacgattgg caccaataga
    15781 ctgataaatg aagccaaagg ccaaccacca aagcacacta atggaaagat actttgtcca
    15841 gcccttgccg tccagtttac gcgcgacaaa ctcctgcttt tccttcttaa actgtgcctg
    15901 ataaatccaa actagaagtc caattggctg catgactgtg aagtaaagtg tcgtcagcac
    15961 ctcaccataa aagcctttct gtagggccaa aataaggtaa ataacagagt taatcaagcc
    16021 aaaaagataa ttacttgctc gaccttccga tacaaagatt acacagataa tccctgtcaa
    16081 gctacaaatc atcccaatcc agtcaacaat acgatgttcg taaaccaact ccagccagag
    16141 aggaaaactt cctaaaacca gcaaataaat ccactgggca aaactacgat gggcaaagag
    16201 gtcatcccag atagccttca tagttcctga aaatcctaaa tcagccatag ccgcaaccat
    16261 acgacggtaa ccacctgaca tttcacctag ggttgttttg atattttcaa ttttcttttg
    16321 caaataagta tgcatcattt ctccttttgt ttttaaagag ccgtgtctgg atagactttc
    16381 ggacgcaacg ctctattaga taatgaactg cctatacaca agatttctaa ccttagtcga
    16441 catgagctga aacctcttat ttgttaagta gttcacaaaa tattatacac ctattttatg
    16501 aatagtcaac tgtctttaca gtaaaatttt agaaaatcat gaaaattttc tctttctttc
    16561 cattttaagt gacattcagt cattctcaca tcaaaaaagc ccagacgaaa ttgtctgagc
    16621 attcttttat ctagtcgttt aaggaagttg agttcagtat gtttaaagtc tctgtcccat
    16681 catttcttca acaaaccttg ttcttggaga aactccttgg ctacttgctt tgctgacttg
    16741 ccttcaacac cgacttggta gttgagctgg ctcatctggc tttctgtaat cttaccagcc
    16801 aatgtattaa gaactctttc caactctggg tgtttcttga gaagagcttc tttcatgagt
    16861 ggagcccctt gataaggtgg gaagagttgc ttgtcatctt ccaagacctg taaatcataa
    16921 cgctccaatt ccgcatcagt cgaataggca tccgtgattt gaatatcccc tgactgaatt
    16981 gcctgataac gaagagctgg ctgcatagtc gccacattga gattgagacc atacattgat
    17041 tgcaagccct tatttccatc ttcacggtcg ttaaactcaa gcgtaaagcc tgccttcagc
    17101 tgcccttcca cttttttcaa atccgaaatg gtcttcaagc catattcttg agcaatcttt
    17161 ttcggaacag ctacagcata ggtattttga taagacatag gtttgagata ggctagatga
    17221 tcctgcttgg caatgccatc acgcgccact tgataaacct gctctggctc atgactcacc
    17281 ttgggtgatg gttgaagcaa actttcagtc accgtaccgg taaattcagg ataaatgtca
    17341 atatcacctt ttttcagagc ttcataaagg aaacttgtcg tcccaaaatt cggtttaaca
    17401 gtcgcagtca tgctggtatt ttcttcaatc agcaatttat acatattagc caaaatttct
    17461 ggttctgggc ctattttccc agcaataatc aagttttcct tctctttttg agccaaaagg
    17521 gctggactat aagacagacc cagtaataaa gccaccaagg caaaaccaga gaaaatcgtc
    17581 cgcaatttcg ctttttccat tacttttagt aggaagttaa aggcaatagc tagcactgca
    17641 gaagaaaggg ccccaatcaa aatcaaactg gcattattac ggtcaattcc caacaggata
    17701 aaggaaccca gtccccctgc cccaatcaag gtcgccaagg tagccgtacc gataatcaag
    17761 accgctgccg tccgaattcc agacatgata acaggcatgg caagtggaat ttcaaacttc
    17821 ttgagacgct cccatctggt catcccaaag gcaatcccag cctcttgaag actcggatca
    17881 attcccttca gcccagtaat agtattttgt aaaatcggaa aaatcgcata aatcactaga
    17941 gctgtcaaag ccggcaaggt cccaattccc atcaaaggga taaagagccc caacaaggcc
    18001 agagacggga tggtctggaa aatccctgca atctgtaaga cccaatccgc cagcttctca
    18061 tgatagcgaa gaaaaacagc caagggaatc gcaagcaaaa tagctagtaa caaggtcaaa
    18121 agtgacaact gcaaatgttg agatagagct gtcaaccaat cactaaaacg atcctgaaaa
    18181 gttgcaatta aattagtcat gaacactacc tccaaacaag tctgctacaa agtctgttgc
    18241 aggcactttt aaaattgtct cgggattcgc tacctggcga atttctccat cctgcaagac
    18301 agcaatacgg tccgccaact tcaaggcttc atccgtatca tgggttacaa aaatcgttgt
    18361 catcccaaac tctttatgca attcttttgt cagaacctgc aactgttttc tcgaaatagc
    18421 atccaaggcc gaaaagggtt catccatgag gaaaatcttg ggctgaccaa tcatagctcg
    18481 gacaataccg acccgttgct gttctccacc agataattca ctaggtaagc gatgcccata
    18541 ctcggctact ggtaaaccaa ccttagccaa aagctcttct gttttcttcg taatttcttc
    18601 cttgctccac cccttcattt caggaatgag agcaatattt tccgcaactg ttagatttgg
    18661 aaaaagagca atagcctgta aaacataacc agtagaaaga cgaagttcac gctcatcata
    18721 gtctttgatg cgcttcccat ccatataaat atttccatca gttggttcca aaagacggtt
    18781 aatcatcttg agcatggtcg tcttacctga cccagaaggc cctactaaaa ccataaattc
    18841 cccatcctca atctgtaagt tgacatctct caagacatcc ttttctgtgt agcgcagtgc
    18901 tacatttttg tattcaatca ttctttgtcc tcaatttaaa acttccctcg attagtcaag
    18961 tcttctacct taggcatgac ttccttatta tcccaatgct ccacaatttt cccgttctct
    19021 aaacggaaga tatcgtactg ggcataagca acgccatcaa tctgagtctg accatagcta
    19081 accacatagt ttccttgtcc taagagttgg aaaacaaagt caaaagtgac actatattca
    19141 gccacatagt ttttataagc agcacttcct tgtccaatct catgattatg ctgaatcaaa
    19201 tcgtctgcca cataatcact ccactgctct agctccccat tttggaaaat ttctgtcaag
    19261 aaacggcgaa ccagcttttt attttctgct ttcttatcca aatccttgat ttcaaaatct
    19321 ccaaaaattt gatctagttg gtcattttca ggtgttcgat agtagtcaat gacatcccaa
    19381 tgctcaacaa tacaaccatt ctcatcctca cggaaagtat ccgtcgtcac ccattgagct
    19441 tctccaccat tcagatattg atgaacatga acaaagacca gattgccatc ctcaatggtg
    19501 cggacaatct taatctgacg ctctggatga cgctcaaaga aatctgcaaa gaaggctgca
    19561 aatccttctt tcccgtcagg aacacctgtc gaatgttgga tataggtatc ccctacagac
    19621 tgggcttgag cctcagcaac tcgcccgtct tgaatggcat ggatgtatag gttgtgagca
    19681 tttttcactt gttgtgacat attctaaacc tcatttccct tctctttcag attcgccaaa
    19741 attctttctt gaaaaccttc aaattggtga atttcttcct ctgaaaatcc tttgtaaaag
    19801 atagtatcca atttctgact gacacgatgc cccacttctt tctggaactt gcctaactcc
    19861 gttaaaacta aatacttctt acgcttgtct tttccacacg gactaacaat tacaagcttt
    19921 tgttcctcta gcttttttat catagtcgtc agcgtattat tcgcaagccc agtcgcaagc
    19981 gcgatatctg tcgcagttgc gcagccagtt tcactattcc ataaaaccgc taaaatcttg
    20041 ccctgttcac ccctataaag agcctcagga tcttgactca gtaacttttg aaaaatccgc
    20101 ccattcaaca aacgaatatg atgggctagc aaatgactat ctttcataac acctccaatt
    20161 tatttcgata tcgaaatgaa taaaataatt gtaacactca tcgttctaac tgtcaactat
    20221 ttcgatttgg aaataatttt tgataattat ccacaccacc atactccggc tcaactaact
    20281 tttaacgaga gtttctaaac tccttcgtcc tccagtctac aaaagccttc cattcgtact
    20341 atcctatatt ttatgagggg acacattttt cctatcagac catttatttt aaagatagaa
    20401 gtaaatcata attgcttcca tctgttcttt tatagtatat tgaagttaga ctagagcact
    20461 gtatcttcta aaacattgat agaaagcgat ttgaatttcc caatcaattt gttcgtattt
    20521 atagcatttt gaaactagaa taggacacca tgactgctaa aagatttcta taaattcatt
    20581 tgattttcct aatcaatttg ttcatatctt atttcattcc gctataattt caccttaccc
    20641 tatctttttc gtagcaccct tcaaacagcc tatcccctac cgtttgacga ttcctcactt
    20701 cgctccactt ccattacaga agtttcttca ctactatggg ctcggctgac ttctcatgat
    20761 tccttgttac tactatttga acgctcacga gatagatctt acaaaaaatg ctttgatcca
    20821 caatggaatc aaagcatttt aaagagttcc tcatacataa gcgcagaagt cgcagttcct
    20881 ctgtacttgg cttcttctct tttgataaag cgagccaagt tgagcaactc aggtgctgga
    20941 tgtttgggat ttaggagcaa ttcacgattg accaggcctg agagacgaac tgcctgcaat
    21001 tgctcatttg tagtaggcag ttttttagca gtctctagga gagcagcaac taaatcttca
    21061 ctcaaatcat gtcgagcatg attgtaaaga tcttttataa ggctttctag gtttggttct
    21121 gccatcccta ccacctccct tatggtttaa taatgtttaa tcaaatcaac cgttgaacga
    21181 tccaatttct tcaccaaggc ttgtaagaaa gcttgcgctt ctaggaagtc atccattgca
    21241 tagagggttt ggtgagaatg gatataacga gcgcagacac cgatagttgt tgatgggaca
    21301 ccaccatttt tcagatgagc tgcacctgca tctgttccgc ctttaccaca gtagtattgg
    21361 tacttgatac cagcttcttc agccgttgtc aaaagaaaat ccttcatccc tgggagaagc
    21421 aagtgacctg gatcatagaa acgaatcaag gttccatctc caatcttgcc ttgaccaccg
    21481 tagacatcac ctgctggtga gcaatcaact gcgaggaaga cttctgggtc aaacttggtt
    21541 gtagaggtat gagcgccacg cagaccaact tcttcttgga cgttagaacc cagatagagt
    21601 tcattgccga gtttttgacc tgataaagct tcagctagct cgcttaccat gaggacaccg
    21661 tagcggttat cccaagcttt tgagatgata tttttttcat tggctgtcaa aattgcagaa
    21721 ctatctggta cgatggtatc accaggacgg atgccaaaac tttctgcctc agccttgtcc
    21781 gcaaaaccac catcaaaaac gatatcggca atggctggca tggttggtcc cccctttcca
    21841 cgagtcaaat gcggaggaac agaacctgaa atcacaggaa tttcatgacc atcacgagtc
    21901 aagagtttga aacgttggct gctaaccacc atggggttcc agccaccgat ttctacgaca
    21961 cggaaggtac catctggctt gatttcgctg accataaaac caacttcgtc catatgagaa
    22021 gcgaccaaga cgcgcggtgc atccacagct tctgaatgtt tgataccaaa aataccaccc
    22081 aagccatctg tcaccacttc atccacatgc ggtgtcaact tttcacgaag ataagcacgg
    22141 acaggcgctt catgacctga gactgcagca agttctgtta cttctttaat ttttgaaaat
    22201 aatgttgtca tttcagttcc ttctttcttt catccatttt accacttttt ataggagaag
    22261 gatagtggga aggtggattt ctaagttagt atcttagtcc tgctctatct tagaaaagga
    22321 tagtattctc ttgcatgtag tgcaaaatct agtaaacatt ccaaaattaa ctcgaatatt
    22381 tatttccaaa caaaaaaaca atacaccatc aaagttgttt gtatttttca tgaaatttac
    22441 agaaaatagt tgacttccct ttcttctttc tttaaatata tagttggttg agtttggaat
    22501 agtacgctgt agctgctgaa acatttctag aaattaattt gactttccta atagagttgt
    22561 tcatatctta tttcaattta ctatagtaca aaactagaaa aggaaaaaat catgaccaaa
    22621 acaattcttg ttacaggtgg gactagctac attggctccc ataccgttaa agctctttta
    22681 aatgctggct ataaggtgca tgtcctcgat aatctctcca caggaaatcg agcggctgtg
    22741 gatagtcgtg ctagctttaa agaactggat gtttatgatg ctagcgcctt aaaggcttac
    22801 ttagaagaaa atcagattga tgctgtcctc cattgtgcag gtgaaattgt tgtgagcgaa
    22861 agtattgaaa atccaagtaa atacttcact gccaacgttg ctggtatgaa ccaagttctc
    22921 aaagtcttat ctgaagttgg cattcaaaaa atcatgttct cttcgactgc ttctctctat
    22981 ggaaataact gtattgacaa gccagtgact gaagataccc tgctcgaccc tgtcaaccct
    23041 tatgcagaga caaaactgat gggcgaacga atgatttact ggatggccaa tcgctacgac
    23101 tggaaatatg ttattttccg ttactttaat gttgctgggg ctgaaatgga tgcttcaaac
    23161 ggtctgcgtg tgaaaaatcc aactcacatc attccaaata tcaacaagac cgcattggga
    23221 caaaatgata gcttaaaaat atttggagat gactacgata cacgtgacgg ttcatgtatt
    23281 cgagattaca tttatgtctt ggaccttgca taggctcatg ttaaaggaat gaactaccta
    23341 tttcaagaag acagttcttc tcaaatcttt aacttaggaa ctaaaaaagg ctatactatc
    23401 aaagaaatct ttaaaactgc tgaagaatta ctaaattaaa aaatcccaca cgagattgtt
    23461 gctcgacgtg ctagtgaccc agccagtgtc ctagcagatg cttcaaaagc taaacaatat
    23521 cttgattgga cggctagcta ctccctcgaa gatattattt tatcagatta aggtaaaaac
    23581 attttagaat aggataacaa aggagaaagt cttgttgctc tctccttgtt gtatttatta
    23641 aattgtcaga aattttattg acagattaga aaaataatgt tacatttata tccgcagata
    23701 tctttcgata ccaaatctac atgaaaggac ggggtatgaa actttctcat tatttaattg
    23761 gcttacttct actcctagtc tttctctcta ttagcattgg gaccagtgat ttttcatggg
    23821 gaaagctatt tgatttcgac cagcagacat ggctcctctt tcaagagtcc cgtctcccaa
    23881 gaactatcag tattctcctg actgcctcta gtatgagtat ggcaggcctt ctcatgcaga
    23941 ctattaccca aaatcagttt gctgcaccga gtacagttgg aacgactgaa gccgccaaac
    24001 tgggaatggt gctgagcctt tttgtctttc catcggctag tctgacccaa aagatgctct
    24061 tcgcttttgt ttcatccatc gtattcaccc tcttcttcct agcctttatg accattttta
    24121 ctgtaaagga aaggtggatg ttgcctctga ttgggatcat ctatagcggg attatcggct
    24181 cagtcacaga agttatcgcc tatcgtttca atctggttca gagtatgact gcctggaccc
    24241 agggctcctt ctccatgatt cagacccatc agtatgagtg gctcttctta ggcctcatca
    24301 tcctgataac cgtttggaaa ttatcccaaa ccttcaccat catgaatctg gggaaagaaa
    24361 tcagcgagag tttggggatt tcctactccc tacttgaaaa actggccctc tttctggtgg
    24421 cgctaacgac aagcgtcacc atgattaccg tgggtggcct accatttctc ggagttatcg
    24481 ttcccaatct tgttcgcaag cgctatggag ataatctaag tcaaaccaaa ctcatggtcg
    24541 cactggttgg tgccaatcta gttctggctt gcgatatcct atcccgagtt ctgattaggc
    24601 cctatgagtt gtctgtcagt ctcttgctag gaatcatcgg tagtctcggc tttatcctac
    24661 ttctctggag agggggacga aaagatgcag actaaaagca aacataccaa gctcttctgg
    24721 attctcatta ttcttgccat cggagcttgt cttctctact tttggcccat cactcacttg
    24781 tcagcctttg cttggaagtt gcgttcccaa aagatcatcg tttatctctt ggtagccatc
    24841 gcgactggga tttcgaccat tagttttcaa accctgacgg aaaatcgctt cctgacgcct
    24901 agtattttgg gaattgaatc cttctacgtc ctactacaaa ccctactact ggtttttgaa
    24961 agcaagtttc ttcaacttgg caaatcccct atcttagaat tcctagtctt acttcttgtc
    25021 cagtccctct tctttctcac cttacaaggt tacttgaaga cactgatgaa gcaagacctg
    25081 gtcttcatcc tgctgatctg tctagcgctc agaagtctct ttcgaaatat cagcaccttc
    25141 cttcaagtcc taatggatcc aaacgaatac gataaactgc aaaatagtct ttttgcctcc
    25201 tttcaacatc tcaacacttc catcctagcc atcggttctc tgatcatcct cgctttgaca
    25261 atctttttct ttcgaaaagc agtcgttcta gatgtcttgc acctgcaaag agaaacggct
    25321 cagatattgg gactcgatgt tgaaaaagaa cagaaagagc tcctctgggg aatcgtgctt
    25381 ttgacctcaa cggccactgc cttggtagga cctatggcct tcttcggctt tatgctggcc
    25441 aacctcacct acctgattgt caaagactat cagcacaagt tactctttat agtggccatt
    25501 ctggttggat ttattagctt aaccttgggg caagccttga ttgaacgagt ctttgcactg
    25561 gaaattcgta tcagtatgat cattgagagt gtgggtggct tcttattctt tatcttacta
    25621 tataggaggt ctcgtcagtg aaactggaaa acattgacaa atccattcaa aaacaggata
    25681 ttttgcaagg catttcgctt gaagtcagtc ctcaaaaact gacagccttt attggtccaa
    25741 atggtgctgg aaaatcgact ctcctctcca tcatgagcag actaaccaag aaagatcagg
    25801 gagttctcag tatcaaagga cgtgaaatcg agagctggaa ttcgcaagaa ctggcccaag
    25861 aactaaccat cctaaaacag aaaatcaatt accaagccaa attgactgtt gaagaactgg
    25921 tcagttttgg acgttttccc tacagccgag gtcgacttag atcagaagac tgggaaaaaa
    25981 tccgagaaac tctgaactat ttggaactga ccaacttaaa agaccgctac atcaatagcc
    26041 tgtcaggggg gcaactccag cgcgtcttta tcgctatggt actggcccag gatacggact
    26101 ttatcttgct ggacgaacca ctcaacaatc tcgatatcaa gcaaagcgtc agcatgatgc
    26161 agattcttcg acgactggtg gaggaactcg gcaagaccat tatcatcgtc ctccacgata
    26221 tcaacatggc cagtcagtat gcagatgaaa ttgtcgcctt caaggacggc caggtcttta
    26281 gcaagggaag caccgatcaa atcatgcagg ctgacctgct cagtcaactt tatgagattc
    26341 ccatcacgct agctgatatc aatgacaaaa agatctgtat ctatagctag taacataaaa
    26401 gctcaagtta gagaaccttc agtctcttag tcaataagat taagagactc cctaaatcgt
    26461 tatcacattt taaaaaggag aaattatgaa aacatccctt aaactttatt tcactgccct
    26521 agtggccagc ttcttgctcc tacttggtgc atgtagtaca aactcaagca ctagtcagac
    26581 agagaccagt agctctgctc caacagaggt aaccattaaa agttcactgg acgaggtcaa
    26641 actttccaaa gttcctgaaa agattgtgac ctttgacctc ggcgctgcgg atactattcg
    26701 cgctttagga tttgaaaaaa atatcgtcgg aatgcctaca aaaactgttc cgacttatct
    26761 aaaagaccta gtgggaactg tcaaaaatgt tggttctatg aaagaacctg atttagaagc
    26821 tatcgccgcc cttgagcctg atttgattat cgcttcacca cgtacacaaa aattcgtaga
    26881 caaattcaaa gaaatcgccc caaccgttct cttccaagca agcaaggacg actactggac
    26941 ttctaccaag gctaatatcg aatccttagc aagtgccttc ggcgaaactg gtacacagaa
    27001 agccaaggaa gaattggcca agctagacaa gagcatccaa gaagtcgcta ctaaaaatga
    27061 aagctctgac aaaaaagccc ttgcgatcct ccttaatgaa ggaaaaatgg cagcctttgg
    27121 tgccaaatct cgtttctctt tcttgtacca aaccttgaaa ttcaaaccaa ctgatacaaa
    27181 atttgaagac tcacgccacg gacaagaagt cagctttgaa agtgtcaaag aaatcaaccc
    27241 tgccatcctc tttgtcatca accgtaccct tgccatcggt ggggacaact ctagcaacga
    27301 cggtgtccta gaaaatgccc ttatcgctga aacacctgct gctaaaaatg gtaagattat
    27361 ccaactaaca ccagacctct ggtatctaag cggaggcgga cttgaatcaa caaaactcat
    27421 gattgaagac atacaaaaag ctttgaaata agccacttca aactcaa
//
LOCUS       DAVBCW010000018        26824 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVBCW010000018 DAVBCW010000000
VERSION     DAVBCW010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26824
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.018"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            403..1581
                     /locus_tag="VQ514_000651"
     CDS             403..1581
                     /locus_tag="VQ514_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2499447.1"
                     /translation="MSNSFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLSQFGKTILLAILVGMFTVMQSVAPLVTYL
                     FVVAISILDGFAAPVSYAIVPRYATDLGKANSALSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTFIILVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRLFVSAN
                     LLEIFSNTIWVSSIILVFVTELLNKTESYWGYSNTAYSIGIIISGLIAFRLSEKFLAA
                     KWESILFPLVAMAIVTLTILYFPNAQMFLLFSALVGMLSQLKEVPESVFLQETVEENH
                     LVNVYSVLEVISTLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK
                     "
     gene            1896..2027
                     /locus_tag="VQ514_000652"
     CDS             1896..2027
                     /locus_tag="VQ514_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HET2499448.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            2048..3598
                     /gene="dltA"
                     /locus_tag="VQ514_000653"
     CDS             2048..3598
                     /gene="dltA"
                     /locus_tag="VQ514_000653"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HET2499449.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEALVQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMIMDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGVTHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFEGLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQSLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            3595..4839
                     /gene="dltB"
                     /locus_tag="VQ514_000654"
     CDS             3595..4839
                     /gene="dltB"
                     /locus_tag="VQ514_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HET2499450.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQTIPERDELMDMLDESVRYIMRGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            4853..5092
                     /gene="dltC"
                     /locus_tag="VQ514_000655"
     CDS             4853..5092
                     /gene="dltC"
                     /locus_tag="VQ514_000655"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HET2499451.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDICVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            5085..6353
                     /gene="dltD"
                     /locus_tag="VQ514_000656"
     CDS             5085..6353
                     /gene="dltD"
                     /locus_tag="VQ514_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HET2499452.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKGELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            6508..6948
                     /gene="adcR"
                     /locus_tag="VQ514_000657"
     CDS             6508..6948
                     /gene="adcR"
                     /locus_tag="VQ514_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HET2499453.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            6948..7652
                     /locus_tag="VQ514_000658"
     CDS             6948..7652
                     /locus_tag="VQ514_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HET2499454.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYSVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            7645..8451
                     /locus_tag="VQ514_000659"
     CDS             7645..8451
                     /locus_tag="VQ514_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET2499455.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            8461..9966
                     /gene="adcA"
                     /locus_tag="VQ514_000660"
     CDS             8461..9966
                     /gene="adcA"
                     /locus_tag="VQ514_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HET2499456.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDVEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISIMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNVAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(11086..11859)
                     /locus_tag="VQ514_000661"
     CDS             complement(11086..11859)
                     /locus_tag="VQ514_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET2499457.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCIYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTLPDQKLLDAVELFDGEWVSDIQ"
     gene            12035..13438
                     /locus_tag="VQ514_000662"
     CDS             12035..13438
                     /locus_tag="VQ514_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HET2499458.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLSQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKKE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGLTEATAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            13553..14191
                     /locus_tag="VQ514_000663"
     CDS             13553..14191
                     /locus_tag="VQ514_000663"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HET2499459.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMELMAEKFKTYGQRK"
     gene            14203..14643
                     /locus_tag="VQ514_000664"
     CDS             14203..14643
                     /locus_tag="VQ514_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HET2499460.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            14636..15067
                     /locus_tag="VQ514_000665"
     CDS             14636..15067
                     /locus_tag="VQ514_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2499461.1"
                     /translation="MFNRGILVASHGDFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVDELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISNYDSKINLEEIVHNAQNSLFNVKQQLNVEEEEDLCL"
     gene            15058..15528
                     /locus_tag="VQ514_000666"
     CDS             15058..15528
                     /locus_tag="VQ514_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2499462.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            15553..16326
                     /locus_tag="VQ514_000667"
     CDS             15553..16326
                     /locus_tag="VQ514_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HET2499463.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            16323..17123
                     /locus_tag="VQ514_000668"
     CDS             16323..17123
                     /locus_tag="VQ514_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET2499464.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            17182..19476
                     /locus_tag="VQ514_000669"
     CDS             17182..19476
                     /locus_tag="VQ514_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HET2499465.1"
                     /translation="MKLWYKKAASNWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELYIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKDCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPANLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFISRVKELKKKLPKTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQEREQAINNWLVSGLHASTQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRI
                     YGKNTDVQNIELVFNSEKIIELNF"
     gene            19486..22602
                     /locus_tag="VQ514_000670"
     CDS             19486..22602
                     /locus_tag="VQ514_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HET2499466.1"
                     /translation="MNKEKIKRKLITILFVCIGMLCFGLLAGVKADNRVQMRTTINNE
                     SPLLLSPLYGNDNGNGLWWGNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDT
                     KELREWYVKMLEEAQSLNIPVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENY
                     WIYNNQLAPHSAKYLEVCAKYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLA
                     TKNTPIRDDAGTDSIVSGFWLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYA
                     SEPESMIAMEMMNVYTGGGTVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAP
                     SKEEVVNRTKAVFWNGEGRISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKE
                     KISSIFPNAKILTKNSEELSSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINK
                     NQQVMLPMYTNNTKSLSLDLTPHTYAVVKENPNNLHILLNNYRTDKTAMWALSGNFDA
                     SKSWKKEELELANWISKNYSINPVDNDFRTTTLTLKGHTGHKPQINISGDKNHYTYTE
                     NWDENTHVYTITVNHNGMVEMSINTEGTGPVPFPTPDKFNDGNLNIAYAKPTTQSSVD
                     YNGDSNRAVDGNRNGNFNSGSVTHTRADNPSWWEVDLKKMDKVGLVKIYNRTDAETQR
                     LSNFDVILYDNNRNEVAKKHVNNLSGESVSLDFKEKGARYIKVKLLTSGVPLSLAEVE
                     VFRESDGKQSEEDIDKITEDKVVSTNKVATQSSTNYEGVAALAVDGNKDGDYGHHSVT
                     HTKADSNAWWQVDLGEEFTVSKVDIYNRTDAEPQRLSNFDVIFLSSSGEEVFRRHFDK
                     VVDGLLSLKVPSVGAKLVKIELKSAAIPLSLAEVEVYGSKRTPKKLSNIALTKETRQS
                     STDYNGFSRLAVDGNKNGDYGHHSVTHTKGDSPSWWEIDLAQTEELEKLIIYNRTDAE
                     IQRLSNFDIIIYDSNNHEVFKQHIDSLESNNLSIDLKGLKGKKVRISLRNAGIPLSLA
                     EVEVYTYK"
     gene            22659..24425
                     /locus_tag="VQ514_000671"
     CDS             22659..24425
                     /locus_tag="VQ514_000671"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HET2499467.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEANDTDIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERAMVWVKEHIKEGVDRNREDLILSKEEKEKQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVERVTGHTLEGRAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            24571..25722
                     /locus_tag="VQ514_000672"
     CDS             24571..25722
                     /locus_tag="VQ514_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET2499468.1"
                     /translation="MATFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKVLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGRPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            25867..26751
                     /locus_tag="VQ514_000673"
     CDS             25867..26751
                     /locus_tag="VQ514_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET2499469.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
BASE COUNT         8385 a         4400 c         5707 g         8332 t
ORIGIN      
        1 atcctctact cgattaactc ccctgaaatt tgttttaatt atgcaaattt cacgtatttt
       61 tgatgctgag acgacgatcc tgggaacttt tcagatattt ttttgactat ctaaaatcta
      121 tcattagaaa agcttagagc gccaaaggat ttgagcgttt ttctgatttt taagactttt
      181 tcccagtctc tttttcgatt gaagatgtaa ttattctact aactaacttc ttagtactag
      241 ccaacaacga taatcataat tcctcctaaa attaggaata ataaaggcaa tagtttttgt
      301 tttttcatgt aaaaaacctc acttttgttt tctgctattt tatgctaaaa tattaaaaat
      361 caaatttaat tccaaagttt gtaactaaag ggggagcgct acatgtctaa ttcatttgtc
      421 aagttgttag tctctcaatt atttgcaaat ttagcagata ttttctttag agtaacaatc
      481 attgctaaca tatacattat ttcaaaatca gtaattgcca catcactagt tcctatctta
      541 ataggaatat cctcttttgt tgcgagtctt ttagttccgt tggttactaa aaggttagcg
      601 ctaaataggg ttttatcttt atctcaattt ggaaagacta tattattggc gatactggta
      661 ggaatgttta ccgtaatgca atccgtagcg cctttggtga cctatctatt tgttgttgca
      721 atttccatac tagatggttt tgcagcaccc gtttcctatg ctattgtgcc acgctatgcg
      781 accgatttgg gtaaggctaa ttcagcctta tcaatgactg gtgaagctgt tcaattgata
      841 ggttggggat taggtggact cttgtttgca acaattggtc tgttacctac cacgtttatc
      901 attttagtct tgtatatcat ttctagcttt ctgatgttat ttcttcctaa cgctgaagtg
      961 gaggtgttag agtcagaaac taatcttgaa attttgctca aaggttggaa gttagttgct
     1021 agaaatccta gattaagact ttttgtatca gcaaatttat tggaaatttt ttcaaatacg
     1081 atttgggttt cttccattat acttgttttt gtaacggagt tattaaataa aacggaaagt
     1141 tactggggat attctaatac agcatactct attggtatta taattagtgg cttaattgct
     1201 tttaggctat ctgaaaagtt ccttgctgct aaatgggaaa gtattctttt tcctcttgta
     1261 gctatggcaa tagtgacact gactattcta tattttccaa acgcacagat gtttttatta
     1321 ttttcagctt tagttggtat gttatcgcaa ctaaaagaag ttcctgaaag tgtatttctt
     1381 caggaaacag tagaggaaaa tcatttagtt aatgtctatt ccgttcttga agtgatttct
     1441 acattagcat tttcagtatt tgttttgcta atgagctata ttactgagag ttttggtatt
     1501 agcatcagtt tttggctatc agccatttgt ctgatgatag aagctatttt aatttatatc
     1561 agacgagatt attttaaatg agagagtggt taaaaagtct ttaaaaatca gaaaagcgca
     1621 tagtatcagg tgttgaaaaa acttgatatt gtgtgtttta ttatggggaa gatttacttc
     1681 attgtctcct gaaatggagt tttgaagcag gatcactcta tttgaaaata tacatagaaa
     1741 aactgtataa tccttaaaat aagctgaaat ctattggaaa catacggtaa acaattgaaa
     1801 agatactata taaagtggta caatggtaga aaaataagct agtaagaatc actcttattt
     1861 tagcgaaaaa ttacagaaat gaatggtttt tcttgatgaa acagagaaaa gaattgtacc
     1921 tctttcttgg tcggacagcc ttgtattttc ttatctttct agggctgctt tacttcttta
     1981 gctatcttgg tcagggtcaa ggaagcttta tctataatga attttaactt gaaggagaat
     2041 ccctattgtg tcaaataaac caatagcaga tatgattgaa accattgagc attttgctca
     2101 gacacagcct agctatcctg tctataatgt tttggggcag gaacacactt atggcgattt
     2161 aaaggctgat tcggatagtt tggctgcagt cattgaccaa ctaggcttgc ctgagaagtc
     2221 tcctgtggtt gtttttggtg gccaagaata tgaaatgttg gcaacctttg tagcgctgac
     2281 taagtcaggt catgcctaca ttccaattga tagccattcg gccttggagc gagtttcagc
     2341 tattttagaa gtagcagagc caagcttgat tattgccatt tcagcctttc ccttggagca
     2401 ggtttctaca ccaatgataa atctagctca ggttcaagaa gcccttgttc aagggaataa
     2461 ctatgaaatc acgcatccag tcaagggaga tgataattac tacattatct ttacttctgg
     2521 tacgactggt aagcctaagg gagtgcagat ttcacatgat aatctcctca gctttacaaa
     2581 ctggatgatt atggataagg aatttgcgac accgagtcgt ccgcaaatgc tggcacagcc
     2641 accttattct tttgacttgt ctgtcatgta ttgggcaccg accttggcac ttggtggtac
     2701 gcttttcact cttccttcag tcatcactca ggactttaag caactctttg cggctatctt
     2761 ttcattgcca atcgctatct ggacatcaac accatccttt gcagatatgg ccatgttgtc
     2821 tgaatacttc aacagtgaga aaatgcctgg agtcacgcat ttctactttg atggtgaaga
     2881 attgacggtc aaaacagctc aaaaactgcg cgagcgtttc ccaaatgccc gtatcatcaa
     2941 tgcttacggc ccaacagaag cgacagtagc tctctcagca gttgccgtga cagacgagat
     3001 gttagcgact ctcaaacgcc taccaatcgg ctataccaag gctgattctc caacctttat
     3061 cattgacgag gaaggaaata aactgccaaa tggcgaacag ggagaaatca ttgtttctgg
     3121 gccagctgtt tcaaaaggtt atatgaacaa tcctgaaaaa acagcagaag ccttctttga
     3181 gtttgaaggt ctgccagcct atcacacagg cgatgtggga accatgacag atgagggctt
     3241 gcttctctac ggcggacgca tggacttcca gattaagttt aacggttacc gcattgagtt
     3301 agaagatgtc tctcaaagcc tcaacaagtc tcgctttatc gaatctgctg tcgcagtacc
     3361 gcgctataac aaggaccaca aggtacaaaa tctattggct tatgtcatct taaaagacgg
     3421 tgttcgtgag cagtttgagc gagatatcga tattaccaag gccatcaagg aagacctgac
     3481 agacatcatg atgtcctaca tgatgccatc taaattcctt taccgagaca gtttgccact
     3541 aactccaaat ggaaagattg acatcaaagg attgattaac gaggtgaata agagatgatg
     3601 gagttttttc aacagcttcc tcatttagag ccatatggca atcctcagta ttttgtttat
     3661 gtgattgctg caaccttgcc catctttata ggcctctttt tcaagaaacg ctttgcctgg
     3721 tatgaagtgt tggtaagtct cttctttatt gtcaccatgt tggtgggtgg aaagaccaat
     3781 caactagctg ccttgggtat ttacctttgc tgggaaatat tgctcctgct tttctacaag
     3841 cattatcgaa aaagcaagga tggcaagtgg gtcttctact tagttagttt tctgtcccta
     3901 cttccgatta tctttgtcaa ggtgcaacca gctatcaatg gaacgcagtc tttgcttggg
     3961 ttcttgggaa tttcttacct gacctttcgt tcggttggga ttatcatcga gctgagagat
     4021 ggagtgatta aggattttac cctctgggaa ttcctccgtt tccttctctt catgccaact
     4081 ttctcgagtg gtccaatcga tcgctttaag cgatttaatg aaaattatca gaccattcct
     4141 gagcgagatg agttgatgga tatgctggat gaatctgtcc gctatatcat gcggggcttt
     4201 ttgtataagt ttatcctagc tcatgtttta ggagagacct tactacctcc tctgaagaat
     4261 ttagccttgc agtcaggtgg cttctttaat ctctatgcct tggcagttat gtatactttt
     4321 ggtctggagc tcttctttga ctttgcaggt tattctatgt ttgctttggc catctcaaac
     4381 ttgatgggaa tccgtagccc tatcaacttt aacaagccct ttttatcaag ggatttaaag
     4441 gagttttgga atcgctggca tatgagtctg tccttctggt tccgtgactt tgtctttatg
     4501 cgaatggtga tggtgttaac cagaaagaaa gtctttaaaa atcgtaatgt aacctcaagc
     4561 atggcctaca ttgtaaatat gctgattatg ggattttggc atggtgtgac ctggtactat
     4621 atcgcctatg gactctttca tggactaggc ttggtcatca atgatgcctg ggttcgcaag
     4681 aaaaaaacgc tcaataagga acggaaaaaa gcagggaagg ctgccctacc tgagaatcgc
     4741 tggattcagt tgcttggtat ggttgtcact ttccatgttg tcatgttgtc attcttaatc
     4801 ttttctggat tcttgaataa tctatggttt aaaaaataaa aggaaataaa atatggatat
     4861 caaatcagaa gttatcgaaa ttattgatga gttgtttatg gaagatgttt ctgacatgat
     4921 ggatgaagat ctttttgatg caggtgtctt ggatagtatg ggaacggttg agttgattgt
     4981 ggagattgag aaccgttttg acatttgtgt ccctgtaaca gagtttggtc gtgacgactg
     5041 gaatacagct aataaaatta tagctggtat tgtggagcta caaaatgctt aaacgcttat
     5101 ggatgatctt cggaccggtc ttgatcgctg gtttgttggt ttttctgctc attttctttt
     5161 atcctactga gatgcatcat aatctaggag ctgaaaagcg ttcagcagtg gctactacta
     5221 tcgatagttt taaggagcga agtcaaaaag tcagagcact atctgatcca aatgtgcgtt
     5281 ttgttccctt ctttggctct agtgaatggc ttcgttttga cggtgctcat cctgcggtat
     5341 tagctgagaa atacaatcgt tcctaccgtc cttatctttt aggacagggg ggagctgcat
     5401 cgcttaacca atattttgga atgcaacaga tgttaccaca gctggagaat aaacaagttg
     5461 tgtatgttat ctcacctcag tggttcagta aaaatggcta tgatccagca gccttccagc
     5521 agtattttaa tggagaccag ttgactagtt ttctgaaaca tcaatctggg gatcaggcta
     5581 gtcaatatgc agcgactcgc ttactgcaac agttcccaaa cgtagctatg aaggacctgg
     5641 ttcagaagtt ggcaagtaaa ggagaattgt cgacagcaga caatgaaatg attgaattat
     5701 tggctcgttt taatgaacgc caagcctcct tttttggtca gttttcggtt agaggctatg
     5761 ttaactacga taagcatgta gctaagtatt taaaaatctt gccagaccag ttttcttatc
     5821 aggcaataga agatgttgtc aaagcagatg ctgaaaaaaa tacttccaat aatgagatgg
     5881 gaatggaaaa ttatttctat aatgagcaga tcaagaagga tttgaagaaa ttaaaggatt
     5941 ctcagaaaag ctttacctat ctcaagtcgc cagagtataa tgacttgcag ttggttttaa
     6001 cacagttttc taaatctaag gtaaacccga tttttatcat tccacctgtt aataaaaaat
     6061 ggatggacta tgctggtcta cgagaggata tgtaccaaca aacggtgcag aagattcgct
     6121 accagttaga aagtcaaggt tttaccaata tagcagattt ttctaaggac ggcggggagc
     6181 ctttctttat gaaggacacc attcaccttg gttggttggg ttggttggct tttgacaagg
     6241 cagttgatcc tttcctatcc aatcccacac cagctccgac ttaccatctg aatgagcgct
     6301 ttttcagcaa agattgggcg acttatgatg gagatgtcaa agaatttcaa tagataatat
     6361 aatatagaag agttcaagaa tgaaactagt tttcacgttt tttcttgact ctttttttgc
     6421 ttgtgatata attaactggt aaacaaaatg tcaaagaata gtatttttct cttaaaaaag
     6481 agaagtctaa aaggaaagga gtcagatatg agacagctag caaaggatat caatgctttt
     6541 ttgaatgagg tgattttgca ggcggaaaat cagcatgaaa tcctaatagg tcattgcact
     6601 agcgaggtgg ccctgaccaa tactcaggag catatcctta tgctcttgtc agaggaatct
     6661 ttaacaaatt cagaattggc ccgtcgtctc aatgtcagtc aggcggcagt taccaaggcc
     6721 attaagtctt tggtcaagga agggatgttg gaaacatcta aagattctaa agatgcgcgt
     6781 gtgatttttt atcagttgac tgacttggct cgtccaatcg ctgaggagca ccaccatcac
     6841 catgagcata cacttttaac ctatgaacaa gtggcgactc agtttactcc aaatgaacaa
     6901 aaagtgattc agcggttttt gactgcttta gtaggagaaa tcaaataatg agatatatta
     6961 cggtagagga tttgtccttc tattatgata aggagcctgt tcttgaacat atcaattata
     7021 gtgttgatag tggggaattt gttaccttga ctggggaaaa tggagcggct aagacgacgc
     7081 tcatcaaggc tagtcttgga attctgcaac cacgcattgg aaaggtggct atttcaaaga
     7141 caaatacgca aggtaagaaa ttgagaatag cctatcttcc tcaacaaatt gccagtttta
     7201 atgctggttt tccaagtacg gtctatgaat ttgtcaagtc gggtcgctat ccgagaaaag
     7261 gctggttccg tcgtttgaat gctcatgatg aggagcatat caaggccagt ctggactcag
     7321 ttggcatgtg ggaacatcga gacaaacgct tggggtctct atctggggga caaaagcagc
     7381 gagcggtgat tgcgcgtatg tttgcttctg accctgatgt gtttatccta gacgagccga
     7441 caacggggat ggatgcagga agtaaaaatg aattttacga actcatgcac cacagcgccc
     7501 atcatcatgg caaggctgtt ttgatgatta cccatgaccc tgaagaagtt aaggattatg
     7561 cggatcgcaa tattcatcta gtccgtaacc aagactcgcc atggcgttgt ttcaacgttc
     7621 atgagaatgg ccaggaggtg ggccatgctt agtttattat cttacgactt tatacaacgc
     7681 gcctttttgg cggttattgc tatgagtctt ttctcaccgg tattgggaac cttcctcatc
     7741 ttgcgtcgtc agagtttgat gagtgatacc cttagccacg tctcactttc aggtgtagcc
     7801 tttggtctgg ttttggggat ttctccaact gtttctacta ttgccattgt cttgattgcg
     7861 gcggtctttc tggagtatct ccgtacggtt tacaagagct ttatggaaat cgggacagct
     7921 atcctcatgt caacaggtct ggctgtttct ctgattgtca tgagcaaggg taaaagctcg
     7981 agttcaatga gtttggacca atatctcttt ggttcgatcg tgactatcag tgaagaacag
     8041 gtcatttccc tctttgtcat tgcggcggtt gttttgattt tgacctttct ctttcttcgt
     8101 cctatgtata tcttaacttt tgacgaagat acggcctttg tggatggctt gccagttcgt
     8161 accatgtcca ttctttttaa catggtgaca ggggtggcta ttgcccttat gattcctgca
     8221 gcaggagctc ttctggtatc gaccattatg gtcttgccag ctagtattgc cctgcgtctg
     8281 gggaaaaact ttaaatcggt tatgctgctt gccagtgcga ttggcttttt gggaatggta
     8341 gcaggacttt acatttccta ctatgcagaa acacctgcaa gtgcaagtat taccattatt
     8401 tttgtaactg tctttatact aatcagttta gtaagacgtt ttatcaaata ggagacgaac
     8461 gtgaaaaaaa ttagcttatt actagccagt ctatgtgcct tgtttttagt ggcttgttcc
     8521 aatcaaaaac aggcagatgg taaactcaat atcgtgacaa ccttttaccc tgtctatgaa
     8581 tttaccaagc aagtcgcagg agatacggct aatgtagaac tcctaatcgg tgctgggaca
     8641 gaacctcatg aatacgaacc atctgccaag gcagttgcca aaatccaaga tgcagatacc
     8701 ttcgtttatg aaaatgaaaa catggaaaca tgggtaccta aattgctaga taccttggat
     8761 aagaaaaaag tgaaaaccat caaggcgaca ggcgatatgt tgctcttgcc aggtggcgag
     8821 gaagaagagg gagaccatga ccatggagaa gaaggtcatc accatgagtt tgatccccat
     8881 gtttggttat caccagttcg tgccattaaa ctagtagagc acattcgtga cagcttgtca
     8941 gcagattatc ctgataaaaa agagaccttt gagaagaatg cagctgccta tatcgaaaaa
     9001 ttgcaagcct tggataaggc ttacgcagaa ggcttgtctc aagcaaaaca aaagagcttt
     9061 gtgactcaac acgcagcctt taactatctt gccttggact atggactcaa acaagtcgca
     9121 atctcaggac tttctccaga tgtagagcca tcagctgctc gcttggcaga attgacagag
     9181 tatgtcaaga aaaataaaat cgcctatatc tattttgaag aaaatgcctc acaagccctt
     9241 gctaacacac tttcaaaaga agcaggtgtc aaaactgatg tcctcaatcc tttagaaagt
     9301 ctgacagaag aggacaccaa ggctggagaa aattacatct ctatcatgga gaaaaacctc
     9361 aaggctttga aacaaacaac agaccaagaa ggcccagcaa tcgagcctga aaaggcagag
     9421 gataccaaga cagtccaaaa tggttacttc gaggatgcag ctgtcaagga ccgcaccttg
     9481 agtgactatg caggtaactg gcaatcagtt tatcctttcc ttgaagacgg cacgtttgac
     9541 caagtctttg actacaaggc taagttgact ggtaagatga cccaggctga gtacaaggct
     9601 tactatacaa aaggctatca gacagatgtg actaagatta acattactga taatactatg
     9661 gaatttgttc aaggtggaca aagcaagaaa tatacttaca agtatgtcgg taagaaaatt
     9721 ttgacttaca agaaaggcaa tcgtggcgtg cgtttcctct ttgaagccac agatgctgac
     9781 gctggacaat tcaagtatgt tcagtttagt gaccacaatg ttgccccagt taaggcagaa
     9841 catttccata tcttttttgg aggtacaagc caagaagctc tatttgaaga aatggataat
     9901 tggccaacct actacccaga taacctatct ggccaagaaa tcgctcaaga aatgttggcg
     9961 cattaatgag aaatcgacta gttcataaga gctagtcggt tttttgatac tagatggcta
    10021 acttcattat agacctttca gaactttaaa caataaataa aactcttgaa atatctgggc
    10081 ttatatgcta gaatgtagtt agtgtataag gttcattaga acaccaaagc ccctaactag
    10141 tgccatagtt aagggctttt ttgacgtatc ttagcgattt aacgggtgtt tataggctag
    10201 tcagtcggtc tacttcttac catctagcca tttgcagacg aaatacaaga taattccagc
    10261 tataacatct gctataatag taagagcaag ctctggtata aggctcatta gtttcacctc
    10321 ctctcacgaa cccataggaa cgtaatcggt aaccgatgac aaaaatagta taccacacta
    10381 aatttagatc atcaacctca cttaatgctt gaactattgt agattatatg ctaaaatgta
    10441 gttaattgat aaggttcatt agaacaccaa agcccctaac tatggccgta gttaagggct
    10501 tttttgacgt atcttagcga tttaacgggt gtttataggc tagtcactcg gtctactcct
    10561 tactatctag ccatctgcag acgaaataca agataattcc agctataaca tccgctataa
    10621 tagtaagagc gagctctatg ataaggctca ttagtttcac ctcctctcac gaacccatag
    10681 gaacgtaatc ggtaaccgat gacaaaaata gtataccaca atatatttag atcatcaagg
    10741 tcacttaatt cttgaaatat agggtctagg cgacaaagtc tttaaaatca gaaaaacgca
    10801 taatatcagg tgtgcaaaaa cttgatacta tgcgttttat tgtgggaagg tttactccat
    10861 tttctcctga aattgagttt ttgtccagcc tctgttttta gaattgctaa gaaaataatg
    10921 tcatgtggtg aatatttgta aatcagtcag cagacagaac gaatactctt cgaaaatctc
    10981 ttcacatcat gtcagcttcg tcttgccgtg tccatggggg acttgacttg atcagttttc
    11041 aaatctgtac tttgagcaag ctgagactag cttcctattt gattttcatt gaatatcaga
    11101 aacccattct ccatcaaata attcgactgc gtctaataat ttttggtctg gcaaggtgtc
    11161 agaaataaag gttgtgtatt tggagagggg attaatttta aaaaatccag tcttgtaaaa
    11221 tttagaacta tcaatcagta agatggtttc atgggctttg tcaataatat tcttttttga
    11281 aatagcttgg ctgagagaag cttcataaac atattggtca tcaatacctc ttgctgaaca
    11341 aaatgctaaa tcgatattaa aatgatctaa taaagaattt tccttatcat agttgaccac
    11401 ggaacaggat tgatgtttga cctcgccaga tgtgataaag attttggagc tatctttaac
    11461 agtttcagat agggtttgtg cagtatgtaa accatttgta aaaataatca aattatcaag
    11521 ttcagaaaga tagggacaaa gttcgtagac agtagtacta gaatctagat agatacacat
    11581 accagaccga ataaagtctt tagcgagact agcgattagt cttttttgcc tagtactttc
    11641 tccttcccgt atttgatgag aaagttcaat tgtgttcata gaggacaggg tcacgtatcc
    11701 gtgctttctt ttgataagac cttgattttc taagaaaatt aaatcacgac gtaaggtact
    11761 tgtgctggag aaagtgattt ctgccagctc ttttacggca attctttttt tctttttaat
    11821 aatttcaatc aattcaagta cacgttcatc ttttatcata agctcctcct aatttatcat
    11881 ttcaactata ttatagcaca aattggagga atttgaatta tttttatgaa tattgggtta
    11941 acatttgaac attattcaag taagcgttca catattgaaa aaataaaacg tggggattat
    12001 aataaagtta atcaaggacg aagagagaag aaaaatggaa gcggttttag caatagattt
    12061 aggtgcgact tctggaagag caatcgttgg ttacctttct gaaaataaac tagtaatgga
    12121 agaaataaat cgcttttcta atctacctat tagagtaaaa gggcatttat cttgggatat
    12181 tgactttcta ctagctaaaa ttcttgaaag tatccgcttg gctaatacta gttacaagat
    12241 tttatctatc ggtattgaca catggggagt tgattttgga ctgattgata atgaaggtaa
    12301 gctgttatca caacctgttc attatcgtga tgaaagaaca aagggagtgt taaaggaaat
    12361 atctgaaatg actgaattag aaaaactgta ttcagagaca ggaaatcaga ttatggagat
    12421 aaataccttg tttcaactct ttaaggcacg tcaagaatct cctgactctt tctataagac
    12481 caataagatt cttttaatgc cagatttgtt taattatctc ttgacaggta agtttgctac
    12541 agaaaaaagc attgcttcaa caactcaatt atttgatcct aggagtcaaa attggaatca
    12601 gaatatctta aaactatttg aattggattc atctttactt cctgaaattg tttcagaggg
    12661 aaatgttctt ggaaggataa aaaaggagta tggtttaggc gatattcctg ttgtgaatgt
    12721 ttgtagtcat gatacagcaa gtgcgattgt ctcagtacct aagacagaag gtagtttatt
    12781 tatttcatca ggtacttggt ctttggttgg agtggaactt acttcaccga ttcttactac
    12841 cgaatccttc agttatggat ttacaaatga agtcggtaaa gatggagtga ttacatttct
    12901 gaagaattgt acagggttgt ggatcataga ggaactaaga cgttcatttg aacgaagagg
    12961 gaaagcctat tcttttgatg atattaggac aatggtggag aaagaaaaag aaaatcttcc
    13021 tctgattgat actgaatcaa ctgaatttgc aacagaatct gatatgcaca agactttgac
    13081 agaatatcta gcttatcatc atgaaactag agagtggaca gatggacaac tatttaagat
    13141 tgtttatgaa agcctagctg aaacgtatag gaaagcgata gagttattag aagaactaac
    13201 tcataaggtt tataagagga tatatgtgat tggaggaggt gctagagcca gttattttaa
    13261 ccaaatgatt gctgatagaa ctggtaaaga ggttcttaca ggtttgactg aggctacagc
    13321 tgtggggaat attgttgtgc agctcatagc tatgggacaa ttaaaaggga tggaagaggc
    13381 tcaccatgtt attgaggagt ttctacaatt agagagttat tactcccaaa agaattaaaa
    13441 agattgagag tttgtaaatt tgcctccctc ccccttctta gcttttgtgc aggaaggggg
    13501 gataattggt gaattgaaaa atatttagtg ttttgatatg aggaggacaa ggatgtcaga
    13561 tgtaaaacaa gaattaatta aatatggtaa gaagctagta gaaacagatt tgacgaaagg
    13621 aacaggtggg aatctcagcg ttttcgatcg tgaaaaacaa ttgatggcaa ttaccccgtc
    13681 gggtattgat ttctttgaaa tcaaagaatc cgatattgta gtgatggata ttaatggaaa
    13741 tgttgtagag ggagaacgct tgccatctag cgaatggtat atgcatttga ttcaatatca
    13801 aactcgtgat gatatcgatg caattatcca tgctcataca acttatgcaa cagtattagc
    13861 ttgtctcaga gaaccacttc cagcgagtca ttatatgatt gcagtggcag ggaaagatgt
    13921 tcgggtagct gagtatgcaa catatggcac gaaagaattg gctgtgaatg cagctaaagc
    13981 aatggaaggt cgtagagcag ttttactagc gaatcatgga attttagcag gtgcacaaaa
    14041 tttattgaat gcatttaata ttgttgaaga agttgaatat tgtgcaaaaa tttattgttt
    14101 agctaagaat tttggagagc cagtagttct tcctgatgag gagatggaat tgatggcaga
    14161 aaaatttaaa acatacggtc agagaaaata gggaggatat taatgttaaa acatataccg
    14221 aaaaatattt ctccagattt attgaagact ttaatggaaa tgggacatgg agatgaaata
    14281 gtattagctg acgcgaatta tccttctgcc tcatgtgcaa ataagctaat tcgttgtgat
    14341 ggcgtaaata ttccagaatt attagattcc attctgtatt taatgccatt agatagttac
    14401 gtcgatagtt caattcagtt tatgaacgtt gtttcgggtg atgatattcc taagatatgg
    14461 ggtacctata gacagatgat tgaaggtcat ggtacagatc ttaaaacgat tacttatctt
    14521 agaagagaag acttttatga acgtagtaag aaagcttatg ctattgttgc tacaggagaa
    14581 acttcacttt atgctaatat tatccttaag aaaggagtag ttgttgaaag agaaaatgtt
    14641 taatagagga attttagttg ccagtcatgg tgattttgct agcggagctc tcatgaccgc
    14701 agaaatgttt gttggtgaga caacaaatga tagagttagg acattaggtt tgatgcctgg
    14761 agagaatatt gtagagtttg agcattattt taaaaatcaa gtggatgaac tgttagactc
    14821 aaatcaagag gttatcgttt tgactgactt gattggagga agtcctaata atgtggcttt
    14881 gtcacggttt ttaaatttgg attcagttga tattgtaaca gggtttaata ttcctctcct
    14941 agtggaatta atatcaaatt atgattcaaa aatcaattta gaagaaattg ttcacaatgc
    15001 tcaaaatagt ttgtttaatg ttaaacaaca acttaacgta gaggaggaag aagatttatg
    15061 tctatagagt ttgttcgtat tgatgaccgt ctggtacatg gtcaagttgt cactacgtgg
    15121 ctaaaaaagt atgatattga gcaagttatc attgttaatg atcgcatctc agaagataaa
    15181 acacgacaat ctattttaaa gatttctgca ccagtaggtt taaaaattgt tttctttagt
    15241 gtaaaacggt ttgtggaagt tttaaactct gtgccaataa aaaagagaac aatgctgata
    15301 tatacaaatc caaaagatgt gtatgattct attgaaggaa atttaaaatt ggagtacctc
    15361 aatgtaggac agatgagtaa aacggaggaa aatgaaaagg taacgggagg tgtagctcta
    15421 ggtgaagaag acaaatatta ttttaagaaa atagttgata agggaacgag agttgaaatt
    15481 caaatggttc ctaatgataa agttacaatg ttggaaaaat ttttataaaa ataatttaag
    15541 gaggtacagt atatgctatt cacacaagca ttactggtga cattagttgg gattattgcc
    15601 actattgact ataatggacc gttatttatg attcaccgtc cgttagttac aagtgcaatg
    15661 gttggcttag tcttaggaga tttcacccaa ggtgttctta ttggttcagc tcttgaatta
    15721 acttggctcg gtgtaacagg tattggaggt tacactccac cagatactat ttcaggtgcg
    15781 attattggta ctgcatttgg tattttatct ggtcaaggag aaactgctgg tatcgctata
    15841 gcagttccaa ttgcagttgc tacccaacag ttggatgttc ttgcaaaaac tttagatgtt
    15901 tattttgtga aaaaagctga taatgatgct aaaaacggag attattcaaa gatcggtttt
    15961 tatcattatt caagtttggt tttaatcacg ttatttaaaa ttgtaccaat tttcctagct
    16021 attatgcttg gaggggaata tgtggcagac ttgtttgcta aggttccacc aatcgttatg
    16081 cagggactta actctgcagg tgctttacta ccttcaattg gttttggtat gcttttaaat
    16141 atgatgctca agaaaaatat gtgggtattc ttgttgattg gattcatttg ttctgtgtat
    16201 ggaggaatgt caaccattgg gatctcacta gttggtattg cggtagcata cttctacgat
    16261 atgattggaa gtaaaccaca agaaacaact tcaagtagtg atgttgagga ggatcttgat
    16321 ctatgatgaa taataaagta actaaagttg aacttaaaaa agttttcaaa cgaagtttta
    16381 tgtatggttc ttcatggaac tatgagagaa tgcagaacct aggttttcta tatacaattc
    16441 ttccagtatt gaaaaaacta tacccagaca aagattcagc ttctcctgca atgaaacgtc
    16501 accttgagtt tttcaatact catcaaacag cggcaccatt tattcttgga gttacttccg
    16561 ctatggaaga acaagaagga aatgaaggtg cagcttcaat tactggtatt aaagttggct
    16621 tgatggggcc actggctggt ctaggagata gtttgttctg gctgacacta gttcctatct
    16681 gttttagtat tggtgcgtct tattctaaag acggcggtgc tttaggtatc tttatcgcct
    16741 taatattgtt taatattatt aatattcctg ttaaatattt cggtttgaaa tatgggtata
    16801 ctaagggttc tagtcttatc caagaaaata atacaaaagg aacattgaat cgcgttacga
    16861 gtatggcgac agcattaggg ctagtactag tgggtggttt gattccatca atggttggta
    16921 ttaattttgg attagaattt aagcaggggg aacttgttat ttctgttcaa gaaatgatta
    16981 caaaattaat tccaggattt atccctatgg ctttgacttt attaatgtgt aaattaatta
    17041 gaaaaggaaa gaatccggtt gtactaatct ttagtgttat ggctattgga gttatcctag
    17101 ttgttttagg aattttgaag tagtagaaag tgtggaggtg gtatttggga tatcacctcc
    17161 attttggaag agaggtaaag agtgaaatta tggtataaga aagctgcctc aaattggaat
    17221 gaagccttgc cgattgggaa cggtcattta ggtggtatga tttatggttc agctacaaaa
    17281 gaatgtattc aactaaacga tgagactatt tggtatagag gaaagtcaga tagaaataat
    17341 ccagactcac tattgcatct taaaaaaatt cgggaatatc ttttagatgg agaaattcag
    17401 aaagccgaag aattgataaa gttaacaatg tttgctaccc caagagatca aagccactat
    17461 gaattacttg gggaacttta cattgagcat atagatattc agtcttgtgc tctttcattg
    17521 tatgaaagag agctagattt agatacagct atttctaatg ttgtgtttga gcctaatagt
    17581 tgtaatttac aaataaaaag agaatatttt acgagtttta ataagaatat tttatgttgc
    17641 cgtatagtgt catcagttca aaacacatta aatttaaaca ttaatttggg tagaaataaa
    17701 cggtttaatg acgaagtatc taaactggat tcaagtacaa ttttaatgtc ggcctctgct
    17761 ggaggtagaa aaggtgttca gtttaaagta gtatgtcatt ctaaggttac ggatggtgaa
    17821 gtaagtgtat tgggagagac aatagttatt cggaatgcta cagaggtatt tctttatctc
    17881 aaatcaatga cggattattg gggaaatata gatatttctt ctcttcaggg agaatttagt
    17941 agtattgatt actttacaga aaaagatgaa catgtaaaaa aatatcagga gcaatttaat
    18001 agagttgatt ttaaactaga ctatagtaaa gattgtctta gcattccaac gaatctactt
    18061 cttgaaaaca ctaaaaagta tagtaactac ttgactaact tgttatttca ttatggaaga
    18121 tatctgttaa tatcgtctag tcaaccgaat ggtttacctg ccaatcttca aggaatatgg
    18181 tgtgatgaat taaatccaat ttggggttct aaatatacga ttaatattaa tactcaaatg
    18241 aattattgga tggtaggtcc atgtgattta ccagaagtag aatatccatt atttgatatg
    18301 ctcgaaagaa tgagagaacc gggaagacta accgctaaga aaatgtatgg agctagaggt
    18361 tttacagcac atcataatac ggatggtttt ggcgatacgg ctccccaatc tcatgccatg
    18421 ggggctgcaa tttgggtatt aactattcca tggttatgta ctcatatttg ggaacactat
    18481 ttatatttcc aagatgagcg tattcttacg gaacattttg aaatgataaa agaagcattt
    18541 cttttctttg aagattattt atttgaggtg gatggctact tgatgacagg tccaagtgtc
    18601 tcaccggaaa ataaatatcg cttaaaaaat ggtattgaag gaaatgcttg tctatcatct
    18661 acaattgata atcaaattct aagatatttt tgtgattcat gcattggcat tgcaaaacaa
    18721 ttaggagaca attcggattt tattagtcgt gtgaaggagt taaaaaagaa actacctaaa
    18781 acaaaaatag gtagtaatgg gcaaatccaa gaatggttag aagattatga agaagtagag
    18841 cctgggcata gacacatttc acctctattt gggctttatc cttataatga gattgatatt
    18901 cataaaactc cggaattagc agaagcagct aaaatcacta tcaataggag attatcaaac
    18961 gctaactttt tatcttcaca ggagagggag caagcgatta ataattggtt agtaagtggt
    19021 ttgcatgcta gtacacaaac aggttggagt gctgcatggc tgattcattt ttttgcgaga
    19081 ctatatcaag gtgaacctgc ttataaccag attaatggtt tgttaaataa tgcgactctt
    19141 ggcaatttat ttcttgacca tccaccattt caaattgatg gtaatttagg tttggtgagt
    19201 ggaatttgtg aattattagt acagagccat cataattggt tatcactaat tccagcttta
    19261 ccttctgctt ggtcagaagg agaagtgaaa ggtttcagag taagaggagg atataaggta
    19321 tcgtttgctt ggaaaaatgg ggatataaca ttcctaaaat tggaaggagg aaacaaagat
    19381 caaaaagtaa gagtaagaat atatggcaaa aatactgatg tacaaaatat tgaattggta
    19441 tttaattcag aaaaaattat tgagttaaat ttttaggtat aagtcatgaa taaagaaaaa
    19501 ataaaaagaa aattaatcac aatactgttt gtatgtattg ggatgttatg ttttggattg
    19561 ttagcaggag ttaaggctga taatcgtgtt caaatgagaa cgacgattaa taatgaatcg
    19621 ccattgttgc tttctccgtt gtatggcaat gataatggta acggattatg gtgggggaac
    19681 acattgaagg gagcatggga agctattcct gaagatgtaa aaccatatgc agcgattgaa
    19741 cttcatcctg caaaagtttg taaaccaaca agttgtattc cacgagatac gaaagaattg
    19801 agagaatggt atgtcaagat gttggaggaa gctcaaagtc taaacattcc agttttcttg
    19861 gttattatgt cggctgggga gcgtaataca gttcctccag agtggttaga tgaacaattc
    19921 caaaagtata gtgtgttaaa aggtgtttta aatattgaga attattggat ttacaataac
    19981 cagttagctc cgcatagtgc taaatatttg gaagtttgtg ccaaatatgg agcgcatttt
    20041 atctggcatg atcatgaaaa atggttctgg gaaactatta tgaatgatcc gacattcttt
    20101 gaagcgagtc aaaaatatca taaaaatttg gtgttggcaa ctaaaaatac gccaataaga
    20161 gatgatgcgg gtacagattc tatcgttagt ggattttggt tgagtggctt atgtgataac
    20221 tggggctcat caacagatac atggaaatgg tgggaaaaac attatacaaa cacatttgaa
    20281 actggaagag ctagggatat gagatcctat gcatcggaac cagaatcaat gattgctatg
    20341 gaaatgatga atgtatatac tgggggaggc acagtttata atttcgaatg tgccgcgtat
    20401 acatttatga caaatgatgt accaactcca gcatttacta aaggtattat tcctttcttt
    20461 agacatgcta tacaaaatcc agctccaagt aaggaagaag ttgtaaatag aacaaaagct
    20521 gtattttgga atggagaagg taggattagt tcattaaacg gattttatca aggactttat
    20581 tcgaatgatg aaacaatgcc tttatataat aatgggagat atcatattct tcctgtaata
    20641 catgagaaaa ttgataagga aaagatttca tctatattcc ctaatgcaaa aattttgact
    20701 aaaaatagtg aggaattgtc tagtaaagtc aactatttaa actcgcttta tccaaaactt
    20761 tatgaaggag atgggtatgc tcagcgtgta ggtaattcct ggtatattta taatagtaat
    20821 gctaatatca ataaaaatca gcaagtaatg ttgcctatgt atactaataa tacaaagtcg
    20881 ttatcgttag atttgacgcc acatacttac gctgttgtta aagaaaatcc aaataattta
    20941 catattttat tgaataatta caggacagat aagacagcta tgtgggcatt atcaggaaat
    21001 tttgatgcat caaaaagttg gaagaaagaa gaattagagt tagcgaactg gataagcaaa
    21061 aattattcca tcaatcctgt agataatgac tttaggacaa caacacttac attaaaaggg
    21121 catactggtc ataaacctca gataaatata agtggcgata aaaatcatta tacttataca
    21181 gaaaattggg atgagaatac ccatgtttat accattacgg ttaatcataa tggaatggta
    21241 gagatgtcta taaatactga agggacaggc ccagtccctt tcccaacacc agataaattt
    21301 aatgatggta atttgaatat agcatatgca aaaccaacaa cacaaagttc tgtagattac
    21361 aatggagact ctaatagagc tgtggatggt aacagaaatg gtaattttaa ctctggttcg
    21421 gtaacacaca ctagggcaga taatccctct tggtgggaag tcgatttgaa aaaaatggat
    21481 aaagttgggc ttgttaaaat ttataatcgc acagatgctg agactcaacg tctatctaat
    21541 tttgatgtga ttctatatga caataataga aacgaagttg ctaagaaaca tgttaataat
    21601 ttgtcgggtg aatctgttag tctagatttc aaagaaaaag gagcaaggta tattaaagtt
    21661 aaattactaa cgagtggagt gcctttgagt ttagcagaag tagaggtttt tagagaatca
    21721 gatggtaagc aatctgaaga ggatatagat aaaataacag aagataaagt agtctctaca
    21781 aataaggtag ctactcaaag ttcaaccaat tatgagggtg tagctgcttt agcagttgat
    21841 ggtaataaag atggagatta cggacatcat tcggtgactc atactaaggc agattctaac
    21901 gcttggtggc aggtcgatct gggagaagag tttacggttt ctaaagttga tatttataat
    21961 agaacagatg ccgaacctca gcgtttatct aattttgatg ttatttttct atcttcatca
    22021 ggagaagaag tttttagaag acattttgat aaagtagttg atggtttgtt atctttaaaa
    22081 gtaccttctg taggggctaa gctagtcaaa atagaattaa aatcagcagc tattccgtta
    22141 agtttagcgg aagttgaagt ctatggttca aagagaactc cgaagaaact ttctaatatt
    22201 gcattaacca aagaaactcg acagagttca acggattaca atggtttttc tcgtctagca
    22261 gttgatggaa ataaaaacgg agattatggt catcattcag tgactcatac caaaggagat
    22321 tctccttcat ggtgggagat agatttagca caaaccgaag aattagaaaa gttaattatt
    22381 tataatagaa cagatgctga aattcagaga ttatcaaatt ttgatattat tatatatgat
    22441 tcaaataatc atgaagtttt taaacaacat attgacagtt tagaaagcaa taatctatcc
    22501 atagacttaa aagggctgaa gggaaaaaag gttagaattt ctttgagaaa cgcaggaatt
    22561 cctttaagtt tggcagaggt agaggtttat acttataagt aattttaaaa attatcatcg
    22621 gtagtatgaa agaaacagaa aaataagagg aaaatagtat gattcaacat ccacgtattg
    22681 gtattcgtcc gactattgat ggtcgtcgtc aaggtgtacg cgaatcactt gaagtacaaa
    22741 caatgaacat ggctaaaagt gtggcagatt tgatttcaag cacattgaaa tatccagatg
    22801 gggaacctgt ggaatgtgtg atttctccat ctaccattgg tcgtgttcca gaggctgcag
    22861 cttcccatga gttgtttaaa aaatcaaatg tttgcgcaac aattacagtt acaccatgct
    22921 ggtgttatgg tagtgaaact atggatatgt ctccagatat tcctcatgct atttggggat
    22981 ttaatgggac agaacgccca ggagctgtct atcttgcagc tgtactagct tcacatgctc
    23041 aaaaagggat tccagccttt gggatttatg gtagagatgt tcaggaagct aatgatacag
    23101 atattccaga agatgtcaaa gaaaaacttt tacgttatgc gcgtgcagct cttgcaactg
    23161 gcttgatgag agacactgct tacctatcaa tgggtagtgt ttcgatgggg attggtggtt
    23221 ctattgtaaa tccggatttc ttccaagaat acttaggaat gcgaaatgaa tcggtagata
    23281 tgacggagtt cacacgccgt atggaccgtg gtatttacga ccctgaagag tttgaacgtg
    23341 ctatggtttg ggtaaaagaa catatcaaag aaggagttga ccgaaatcgt gaggatttga
    23401 ttctttctaa agaagagaaa gaaaaacaat gggaatttgt tattaagatg ttcatgattg
    23461 gacgtgactt aatggttggt aacccaagac ttgctgaact tggttttgag gaagaagcag
    23521 ttggtcacca tgctttagta gctggtttcc aaggtcaacg tcagtggaca gaccattttc
    23581 caaatggaga ttttatggaa actttcctca atactcagtt tgactggaat ggtattcgaa
    23641 aaccatttgt atttgcgaca gagaatgatt cactaaatgg tgtgtctatg ctctttaatt
    23701 atctattaac aaatactcca caaatctttg ctgatgtgcg tacttattgg agtccagagg
    23761 ctgttgaacg tgtaacagga catactttag agggtcgtgc tgcagctggc ttcttacatc
    23821 taatcaactc tggatcttgt acattggatg gtacaggtca agctactcga gatggcaaac
    23881 ctgttatgaa accattctgg gagttggaag aaagtgaagt gcaggctatg cttgaaaata
    23941 cagacttccc accagcaaac cgcgaatact tccgtggagg aggattctca actcgtttct
    24001 tgacgaaggg ggatatgcca gtaacaatgg tacgtctcaa tcttctaaaa ggggttggtc
    24061 cagtgctaca aattgcagaa ggttacacac ttgaacttcc tgaagatgtt caccatactt
    24121 tagataatcg tacagatcca ggatggccaa ctacttggtt tgctccacgt ttgacaggaa
    24181 aaggtgcttt caagtctgtc tatgacgtca tgaataattg gggagctaat cacggagcca
    24241 taacatatgg ccacattgga gcagacttga ttaccttggc ttctatgttg agaattcctg
    24301 tcaatatgca taatgtacct gaggaagata tctttagacc taaaaattgg tccttatttg
    24361 gaacagaaga tctagaatca gcagactatc gtgcatgtca gttgttggga ccactacata
    24421 aataaaactt gtttatatag gaggtgaact tacgtccctc ctatcctttt aaaaagattt
    24481 gttaaacaat tcacaaataa ttgaaaacga atacaaaaag taatataatg atgttaaata
    24541 gatagcgcaa aggcgcagga ggaaaattat atggctacat tttatgttcc ggcagtcaac
    24601 cttattggta aaggtgttgt aaatgaagtg ggtccttata tcaaggaact tggctataaa
    24661 aaggcacttt tggttacaga taagtacatc gaaggcagtg acattttacc taaggtttta
    24721 aaaccactgg atacagaagg aatcgaatat gtcatcttta gcgatgtaga gccaaaccct
    24781 acttgtaaga atgtcacaga tggggtagct gctttgcaag aacatggatg tgattttatc
    24841 atcagtcttg gtgggggttc tccacaggat gcagctagtt gtatttctat catggctaca
    24901 aatggtggaa gaccacagga ttatgaaggg cttcataagt ctgctaaaaa aggcttgcca
    24961 gttgtggcta tcaatacaac ggcaggtaca tcagcagaaa ttaccattaa ctatgtgatt
    25021 actgatgaag aacgcaaggt taagatggta atggttgaca agaatagcct tgccttgatt
    25081 tctgtcaatg accctgaact catgctttcc aaacctaaag gcctgactgc tgctactggt
    25141 atggatgctc tgactcatgc tgttgaagct ttggtaacac ctggtgctta tgatgtaacc
    25201 aagaaactgt ctattggtgc tattgagctt atcaaggaat atcttcctcg tgctgtagaa
    25261 aatggacatg atattgaagc gcgtgaaggt atggtcaatg ccatcttcct tggtggtatg
    25321 agctttaata atgctggtct tggctatgtt cactcaatgg ctcaccaact cggtgcagta
    25381 tataatttgc cacatggcgt gtgctgtgcc atgttgctac cagttataga acgtgaaaat
    25441 gctaaacgtg taccagaagc tttccgcaat gttgccaaag ccttgggact tcatgtagaa
    25501 ggtaaatcag atcaagaatg tgccgattat gcgattgctg agattgagaa gctttctgag
    25561 acagtaggta ttcctaagaa actgactgaa cttggtattg aagaaaaaga tttcgacttt
    25621 gaataccttt ctaagaatgc cttgattgat gcctgcgcac caggaaatcc atttatgcca
    25681 accttagaag aaacgattgc cttttataaa gagttatttt agcaagagat tggacgacaa
    25741 taggacaatt cacctctaat tattgacatt tcaggaaaaa tcgcagtaaa aacggcacag
    25801 ccttctttaa atgtgctata atactagaaa aatacttgtg gaggttccat tatggcaata
    25861 tttttcatga tttttctgat tgtttgtgtg ctcctattgg tgatagtcac actgagtaca
    25921 gtttatgtgg ttcgtcagca gtcggtggcg attattgaac gctttgggaa ataccaaaag
    25981 gttgccaata gcggtattca tattcgcttg ccttttggga ttgactcgat tgcagcacgg
    26041 attcagttgc gcttgttgca aagtgatatt gtggttgaga ctaagaccaa ggacaatgtg
    26101 ttcgttatga tgaatgtagc gactcagtac cgtgtcaacg agcagagcgt gacagatgct
    26161 tactataaac tcatacgtcc agaatctcag attaaatctt atatcgaaga tgctcttcgt
    26221 tcttctgttc caaaattaac cttggatgaa ttgtttgaga aaaaagatga gattgccctt
    26281 gaagttcaac accaagtagc agaagaaatg accacttacg gctacattat cgtgaaaacc
    26341 ttgattacca aggtcgaacc agatgcagaa gttaagcaat ctatgaatga aatcaatgcg
    26401 gcgcaacgta agcgggtcgc agcacaagaa ttggcggaag ctgacaagat taaaattgtc
    26461 actgcagctg aagccgaagc agaaaaagac cgccttcatg gtgtggggat tgcccaacaa
    26521 cgtaaggcga ttgtggatgg attggcagag tctatcaccg aactcaagga agccaatgtt
    26581 ggaatgacag aagaacaaat catgtctatc ctcttgacca accagtattt ggataccttg
    26641 aatacctttg cctctaaagg aaatcaaacc atctttttac caaatactcc aaatggtgtg
    26701 gatgatatcc gaacacaaat cttgtcagcc cttcgcgctg agaagaaata atagactaat
    26761 actcttcgaa aatctcttca aaccacgtca gcgtcgcctt gccgtataga tgttactgac
    26821 ttcg
//
LOCUS       DAVBCW010000019        22892 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVBCW010000019 DAVBCW010000000
VERSION     DAVBCW010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22892
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.019"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(50..274)
                     /locus_tag="VQ514_000674"
     CDS             complement(50..274)
                     /locus_tag="VQ514_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory protein Asp5"
                     /protein_id="HET2499470.1"
                     /translation="MIEMLIVLAIILSLALIVLVTIQPRQNQLFSMDATSNIGKPSYW
                     QSNTLVKVLTLLVSLALFVLLLTFMVITYK"
     gene            complement(279..458)
                     /gene="asp4"
                     /locus_tag="VQ514_000675"
     CDS             complement(279..458)
                     /gene="asp4"
                     /locus_tag="VQ514_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory system protein Asp4"
                     /protein_id="HET2499471.1"
                     /translation="MSEEDLFYKDVEGRMEELKQKPIKKEKETRGEKISKTFSLLLGL
                     MILIGLLFTLLGILR"
     gene            complement(460..1797)
                     /gene="gtfB"
                     /locus_tag="VQ514_000676"
     CDS             complement(460..1797)
                     /gene="gtfB"
                     /locus_tag="VQ514_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HET2499472.1"
                     /translation="MIELYDSYSQESRDLHESLVATGLSQLGVVIDADGFLPDGLLSP
                     FTYYLGYEDGKPLYFNQVPVSDFWEILGDNQSACIEDVTQERAVIHYVDGMQARLVKQ
                     VDWKDLEGRVRQVDHYNRFGACFAKTTYSADSEPIMTVYQDVNGQQVLLENHVTGDIL
                     LTLPGQSMRYFANKVEFITFFLQDLEIDTSQLIFNTLATPFLVSFHHPDKSGSDVLVW
                     QEPLYDAIPGNMQLILESDNVRTKKIIIPNKATYERALELTDEKYHDQFVHLGYHYQF
                     KRDNFLRRDALILTNSDQIEQVEAIVEALPDVTFRIAAVTEMSSKLLDMLRYPNVVLY
                     QNASPQKIQELYQLSDIYLDINHSNELLQAVRQAFEHNLLILGFNQTVHNRLYIAPEH
                     LFESSEVAALVETIKLALSDVDQMRQALGKQGQHANYVDLVRYQEIMQTVLGG"
     gene            complement(1790..3301)
                     /gene="gtfA"
                     /locus_tag="VQ514_000677"
     CDS             complement(1790..3301)
                     /gene="gtfA"
                     /locus_tag="VQ514_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HET2499473.1"
                     /translation="MTIYNINLGIGWASSGVEYAQAYRAGVFRKLNLSSKFIFTDMIL
                     ADNIQHLTANIGFDDNQVIWLYNHFTDIKIAPTSVTVDDVLAYFGGEESHREKNGKVL
                     RVFFFDQDKFVTCYLVDENKDLVQHAEYVFKGNLIRKDYFSYTRYCSEYFAPKDNVAV
                     LYQRTFYNEDGTPVYDILMNQGKEEVYHFKDKIFYGKQAFVRAFMKSLNLNKSDLVIL
                     DRETGIGQVVFEEAQTAHLAVVVHAEHYSENATNEDYILWNNYYDYQFTNADKVDFFI
                     VSTDRQNEVLQEQFAKYTQHQPKIVTIPVGSIDSLTDSSQGRKPFSLITASRLAKEKH
                     IDWLVKAVIEAHKELPELTFDIYGSGGEDSLLREIIANHQAEDYIQLKGHAELSQIYS
                     QYEVYLTASTSEGFGLTLMEAIGSGLPLIGFDVPYGNQTFIEDGQNGYLIPSSSDHVE
                     DQIKQAYAAKICQLYQENRLEAMRAYSYQIAEGFLTKEILEKWKKTVEEVLHD"
     gene            complement(3313..5685)
                     /gene="secA2"
                     /locus_tag="VQ514_000678"
     CDS             complement(3313..5685)
                     /gene="secA2"
                     /locus_tag="VQ514_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HET2499474.1"
                     /translation="MFRRLGQDFQLRKVKKILKQINALKGKMSSLSDQELVAKTVEFR
                     QRLSEGESLDDILVEAFAVVREADKRILGMFPYDVQVMGAIVMHYGNVAEMNTGEGKT
                     LTATMPVYLNAFSGEGVMVVTPNEYLSKRDAEEMGQVYRFLGLTIGVPFTEDPKKEMK
                     AEEKKLIYASDIIYTTNSSLGFDYLNDNLASNEEGKFLRPFNYVIIDEIDDILLDSAQ
                     TPLIIAGSPRVQSNYYAIIDTLVTTLVEGEDYIFKEEKEEVWLTTKGAKSAENFLGID
                     NLYKEEHASFARHLVYAIRAHKLFTKDKDYIIRGNEMVLVDKGTGRLMEMTKLQGGLH
                     QAIEAKEHVKLSPETRAMASITYQSLFKMFNKISGMTGTGKVAEKEFIETYNMSVVRI
                     PTNRPRQRIDYPDNLYITLPEKVYASLEYIKQYHAKGNPLLVFVGSVEMSQLYSSLLF
                     REGIAHNVLNANNAAREAQIISESGQMGAVTVATSMAGRGTDIKLGKGVAELGGLIVI
                     GTERMESQRIDLQIRGRSGRQGDPGMSKFFVSLEDDVIKKFGPSWVHKKYKDYQVQDM
                     TQPEVLKGRKYRKLVEKAQHASDSAGRSARRQTLEYAESMNIQRDIVYKERNRLIDGS
                     RDLEDVVVDIIERYTEEVAADHYASRELLFHFIVTNISFHVKEVPDYIDVTDKTAVRS
                     FMKQVIDKELSEKKELLNQHDLYEQFLRLSLLKAIDDNWVEQVDYLQQLSMAIGGQSA
                     SQKNPIVEYYQEAYAGFEAMKEQIRADMVRNLLMGLVEVTPKGEIVTHFP"
     gene            complement(5678..6118)
                     /gene="asp3"
                     /locus_tag="VQ514_000679"
     CDS             complement(5678..6118)
                     /gene="asp3"
                     /locus_tag="VQ514_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HET2499475.1"
                     /translation="MIITQRQSIYWGEVGGTYMYGTTVSYYLDKSVRLYNPLLPSGEI
                     LKTWFSSVNYQAARTQPQLPLLKRKQEYQLSLVFDCQPENGVYTKITFFDRYGDILEK
                     KVEKAKDFIFTYPEDSYTYQVSLLSAGFESLTFYHFSIKEIRSV"
     gene            complement(6115..7650)
                     /gene="asp2"
                     /locus_tag="VQ514_000680"
     CDS             complement(6115..7650)
                     /gene="asp2"
                     /locus_tag="VQ514_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HET2499476.1"
                     /translation="MSKELNILQIGLANWENHYDIPENMSWYYFYPNSSKTLREIIEK
                     EDINRFHAVLIEDGQYSRDLFSYVKYFEPYTLFYNQNLQINDREVVDFLKKRCAQAID
                     FLSPQQLINDLSKSLFGGGYGDKLFPPTIQVNPNFTGAISYQGLDYVSLEGEFGQDFA
                     QLAYWAYNIMVQKTLPIELWLEYEKEGNCDFRLVIRKMWSGSVDDFFEEVIVSEKDLE
                     QALFMDSRDGDYFLSISVEARGRGTIKLGNLHQRWSRKQFGKFVLGGNILHDSKRDEI
                     NYFFHPGDFKPPLTVYFAGYRPAEGFEGYFMMKTLGCPFILFSDPRLEGGAFYLGTDE
                     LEGKVKDTITHYLDYLGFDHKDLILSGLSMGTFPALYYGASFEPHAIIVGKPLANLGT
                     IASRGRLDAPGVSNLAFDCLIHHTGGTSSQDMTELDQRFWKIFKQANFSKTTFGLSYM
                     KDEEMDPQAYEQLVSYLCNTGAKILSKGTAGRHNDDTDTNISWFLHFYRMVLETGFGR
                     EKR"
     gene            complement(7634..9214)
                     /gene="asp1"
                     /locus_tag="VQ514_000681"
     CDS             complement(7634..9214)
                     /gene="asp1"
                     /locus_tag="VQ514_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HET2499477.1"
                     /translation="MYYFIPAWYGSERTWHADITPWYFSHFRLEFDDTFHQIRLFQEQ
                     DIDSRLLVLAYQPHLRYFLYRHGVLETDTYSVFDVMQDFHNLHTQVLSIRDIEWDDDC
                     EFIYSPFTIIVQKNGKKFAKVEHGVEGFISDIQYFEPNGQIHMHHIMDDRGFISSIIF
                     FEDGQAAYQEYLNPKGVWQFRERLKEGGQVEVNPILGYRFKMLTYQNMGDLVAEFFEN
                     YLQTYVKDQDIFMLPSHSHHDQLVLDRLPSTNPKLLSLFIGRNPQDTFRDLDVTFEKS
                     DLILVDREDSLRLLQELYPERMHQCYHLSSFDTRLRLGRSQTKKESIIYFQLDFEQGI
                     DNQALLQVLSFVAENKDTEVIFGAFAASQEQMNEVEGIVESFIQENIQSENLGKAIDY
                     GDAENPLEENQHQDLRLQFVNLNDELDLIKTLEFVRLIVDLNRHPHLYTQIAGISAGI
                     PQINLVETVYVEHLKNGYLLTDVTEFSKAAHYYTDRLKEWNESLIYSIDKIKEHTGQQ
                     FLGKLEKWIEEVKNVKGT"
     gene            complement(9224..10441)
                     /gene="secY2"
                     /locus_tag="VQ514_000682"
     CDS             complement(9224..10441)
                     /gene="secY2"
                     /locus_tag="VQ514_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HET2499478.1"
                     /translation="MTKIYSSIAVKKGLFTLFLLFIYVLGSRITLPFVDLNTKDFLGG
                     STAYLAFSAALTGGNLRSLSIFSVGLSPWMSAMILWQMFSFSKRLGLTSTSIEIQDRR
                     KMYLTLMIAVIQSLAVSLRLPVQSSYSAILVVLMNTILLIAGTFFLVWLSDLNASMGI
                     GGSIVILLSSMVLNIPQDVLETFQTVHIPTGIIVLLALLTLVFSYLLALMYRARYLVP
                     VNKIGLHNRFKRYFYLEIMLNPAGGMPYMYVMSFLSVPAYLFILLGFIFPNHSGLAAL
                     SKEFMIGKPLWVYVYISVLFLFSIIFAFVTMNGEEIADRMKKSGEYIYGIYPGADTSR
                     FINRLVLRFSVIGGLFNVVMAGGPMLFVLFDEKLLRLAMIPGLFMMFGGMIFTIRDEV
                     KALRLNETYKPLI"
     gene            complement(10498..11175)
                     /locus_tag="VQ514_000683"
     CDS             complement(10498..11175)
                     /locus_tag="VQ514_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET2499479.1"
                     /translation="MGISVAKGEYITIVDSDDIVKENMIETLYQQVQEKDADVVIGNY
                     YNYDESDGNFYFYVTGQDFCVEELAIQEIMNRQAGDWKFNSSAFILPTFKLIKKELFN
                     EVHFSNGRRFDDEATMHRFYLLASKIVFINDNLYLYRRRSGSIMRTEFDLSWARDIVE
                     VFSKKISDCVLAGLDVSVLRIRFVNLLKDYKQTLEYHQLTDTEEYKDICFRLKLFFDA
                     EQRNGKS"
     gene            complement(11160..11405)
                     /locus_tag="VQ514_000684"
     CDS             complement(11160..11405)
                     /locus_tag="VQ514_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2499480.1"
                     /translation="MSELISVVVPIYNTGKYLVECVEHILKQTYQNIEIILVDDGSTD
                     NSGEICDAFMMQDNRVRVLHQENKGGGQHKLKIWGLV"
     gene            complement(11405..12601)
                     /locus_tag="VQ514_000685"
     CDS             complement(11405..12601)
                     /locus_tag="VQ514_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HET2499481.1"
                     /translation="MRKSIVLAADNAYLIPLETTIKSVLYHNRDVDFYILNSDIAPEW
                     FKLLGRKMEVVNSTIRSVHIDKELFESYKTGPHINYASYFRFFATEVVESDRVLYLDS
                     DIIVTGELATLFEIDLKGYSIGAVDDVYAYEGRKSGFNAGMLLMDVAKWKEHSIVNSL
                     LELAAEQNQVVHLGDQSILNIYFEDNWLALDKTYNYMVGVDIYHLAQECERLDDNPPT
                     IVHYASHDKPWNTYSISRLRELWWVYRDLDWSEIAFQRSDLNYFERSNQSKKQVMLVT
                     WSADIKHLEYLVQRLPDWHFHLAAPCDCSEELTSLSQYTNVTVYQNVLHSRIDWLLDD
                     SIVYLDINTGGEVFNVVTRAQESGKKIFAFDITRKSMDDGLYDGIFSVERPDDLVDRM
                     KNIEIE"
     gene            complement(12610..13830)
                     /locus_tag="VQ514_000686"
     CDS             complement(12610..13830)
                     /locus_tag="VQ514_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HET2499482.1"
                     /translation="MRNTKRAVAFAGDYAYIRQIETAMKSLCRHNSHLKIYLLNQDIP
                     QEWFSQIRIYLQEMGGDLIDCKLIGSQFQMNWSNKLPHINHMTFARYFIPDFVTEDKV
                     LYLDSDLIVTGDLTDLFELDLGENYLAAARSCFGAGVGFNAGVLLINNKKWGSETIRQ
                     KLIDLTEKEHENVEEGDQSILNMLFKDQYSSLEDQYNFQIGYDYGAAAFKHQFIFDIP
                     LEPLPLILHYISQDKPWNQFSVGRLREVWWEYSLMDWSVILNEWFSKSVKYPSKSQIF
                     KLQCVNLTNSWCVEKIDYLAEQLPEVHFHIVAYTNMANELLALTRFPNVTVYPNSLPM
                     LLEQIVIASDLYLDLNHDRKLEDAYEFVLKYKKPMIAFDNTCSENLSEISYEGIYPSS
                     IPKKMVAAIRSYMR"
     gene            complement(13861..16305)
                     /locus_tag="VQ514_000687"
     CDS             complement(13861..16305)
                     /locus_tag="VQ514_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_1767 family glycosyltransferase"
                     /protein_id="HET2499483.1"
                     /translation="MIKTIVLAGDRNYIRQLETTIKSILYHNRDVKIYILNQDIMPDW
                     FRKPRKIARMLGSEIIDVKLPEQTVFQDWEKQDHISSITYARYFIADYIQEDKVLYLD
                     SDLIVNTSLEKLFSICLEEKSLAAVKDTDGITFNAGVLLINNKKWRQEKLKERLIEQS
                     IVTMKEVEEGRFEHFNGDQTIFNQVLQDDWLELDKEFNLQVGHDVTAFYNKWENYFNE
                     LVPPSIIHFVSYRKPWTTLIANRYRDLWWEFHDLEWTKILQHHIGEFELTSSLDKEFS
                     CLTLTNSQDLEGIEELVTALPDVVFHIAAWTDMGDKLIKLAVYDNVRLHPQIVPPVLD
                     KLKKSTNLYLDINHGSADENFLKSLQEQEKTLLAFQSTQHGELGQIVFENGKVSFMID
                     TIKDFKKNGHLTCFRQLPSLTCLTFTASQDIEQLDYLAGQLPNVVFQVAAWTAMGPKL
                     YDLSNRYPNIQLYPAISRDKLDELKEKMDAYLDINLLTSTSDIVAEMAHLSKPILAFY
                     KSQNGNNGQRLYSSEHPERMLADLQKLITKDMLEKPLDIIQVKGIDETLDYIIEHNSS
                     LVRFGDGEINMLAGHSIPYQDYDEELVSIMRDIIGQESREDLVVCLPDAFTDRFRFTS
                     WAIPFWKDHMDHYMDFYRELCSDSWYGSTFVSRPYIDFEDKSQAKAQFEKLKSIWENR
                     DLLIVEGATSRSGVGNDLFDEANSIKRIICPSHSAFSRVHEIEQEIEKYAGGRLILCM
                     LGPTAKVLSYNLCQMGYQVLDVGHIDSEYEWMKMGAKTKVKFSHKHTAEYNFDQDIEF
                     IDDETYNSQIVARILN"
     gene            complement(16315..17325)
                     /locus_tag="VQ514_000688"
     CDS             complement(16315..17325)
                     /locus_tag="VQ514_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET2499484.1"
                     /translation="MKLHLTNLYGMAGDSTVILAQNAVQKIASQLGFREVGIYFYNIA
                     SDSPSEMNKRLDGIMASISIGDILVFQSPTWNGFEFDRLLFDKLKDMQVKIICFIHDV
                     VPLMFDSNYYLMKDYMYMYNLSDVLIVPSERMKTRLMEEGLTTKKILVQGMWDHPHDL
                     SLYTPAFKKELFFAGSLERFPDLQNWSQDTPLRVFSNKGEASSSARNLSIEGWKKDEE
                     LLLELSKGGFGLVWGTYQNDGESNQYYTLNISHKVSTYLTAGIPVIVPSSLSTAKFIV
                     DQGLGFVANSLEEVHAIVDKMNLQEYQEMTNRIKTFSYLLKEGYFTKKLLVDAIYHLG
                     VE"
     gene            complement(17354..18229)
                     /locus_tag="VQ514_000689"
     CDS             complement(17354..18229)
                     /locus_tag="VQ514_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2499485.1"
                     /translation="MENELISIVVPIYNVENYLRQCLDSIQKQTYPNFECIMVNDGSP
                     DHSSKICEEFVEKDSRFKYFEKANGGLSSARNLGIECSGGAYITFVDSDDWLEHDALD
                     RLYGALKKENADISIGRYNSYDETRYVYMTYVTDPDDSLEVIEGKAIMDREGVEEVRN
                     GNWTVAVLKLFKRELLQDLPFPIGKIAEDTYWTWKVLLRASRIVYLNRCVYWYRVGLS
                     DTLSNTWSEKRMYDEIGAREEKIAILASSDYDLTNHILIYKNRLQRVIAKLEEQNMQF
                     TEIYRRMMEKLSLLP"
     gene            complement(18269..19147)
                     /locus_tag="VQ514_000690"
     CDS             complement(18269..19147)
                     /locus_tag="VQ514_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HET2499486.1"
                     /translation="MKKNHLVGDALILTVSDQIEELDYLLESLPNICFYIAAPVQFSE
                     KIRSLETNYNVRLRTITNEEQLNFLVDTCDFLLDINHFQEVDAIVSKFVQAGKSVFAF
                     DNTVHGNQGQEVFLSSTPDKLVSRVRDYLNEVRVGTNHQEKIIQDGTWNVFKIDDKAH
                     FIVGANVICRNFENFHVSSGKLILHDGVFINNSCSFNCMERIEIGAGTMMGEGVRFYD
                     HDHIYTAEKIEKWQWTTAPIRVGRDCWIGSNVTILKGVTIGDNTIIGAGCLIRNDIPS
                     NSVVYNNGNLVVKRRD"
     gene            complement(19144..21229)
                     /locus_tag="VQ514_000691"
                     /pseudo
     CDS             complement(19144..21229)
                     /locus_tag="VQ514_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000341103.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(21290..>22892)
                     /locus_tag="VQ514_000692"
     CDS             complement(21290..>22892)
                     /locus_tag="VQ514_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET2499487.1"
                     /translation="SASASASTSASTSASTSASASASISASESASTSASASASTSASA
                     SASTSASASASTSASASASTSASASASTSASASASTSASASASTSASASASTSASESA
                     STSASASASTSASASASTSASASASTSASASASTSASESASTSASASASTSASASAST
                     SASASASTSASESASTSASTSASASASTSASASASTSASTSTSTSASTSASTSASTSA
                     STSASTSASESASTSASTSASTSASASASISASESASTSASASASTSASASASTSTSA
                     SESASTSASASASTSTSASESASTSASASASTSASASASTSASASASTSASESASTST
                     SASESASTSASASASTSASASASTSASASASTSASASASTSASASASTSASASASTSA
                     SASASTSASASASTSASASASTSASASASTSASASASTSASASASTSASASASTSASE
                     SASTSASESASTSASASASTSVSNSANHSNSQVGNASGSTGKSQKELPNTGTESSIGS
                     VLLGVLAAVTGIGLVAKRRKRDEEE"
BASE COUNT         7174 a         4884 c         3759 g         7075 t
ORIGIN      
        1 aagaaaactt cagaccaatc cacaaaaggt gaatatctga agttttcttt tatttataag
       61 taatcaccat aaaggttaat agtagaacaa ataaagccaa actcaccaat aaagtgagca
      121 ccttgaccaa ggtgttgctc tgccagtagc ttggtttacc aatattacta gtggcatcca
      181 tggaaaatag ttgattttga cggggttgta tagttaccaa tacaatcaaa gcaagagata
      241 ggataatagc taaaacaatt agcatttcaa tcattgatct acctcaaaat tcccaacaaa
      301 gtaaagagca aaccaatcag aatcattaag cccagtaaaa gtgaaaaagt tttactaatc
      361 ttctcccctc gggtttcttt ttccttcttg atgggttttt gtttcaactc ttccatgcga
      421 ccttcaacgt ctttgtaaaa taaatcttcc tctgacatgt tagcctccta aaacagtttg
      481 cattatttct tgatatcgca ccaagtcaac ataatttgca tgttggcctt gtttgccaag
      541 tgcctgacgc atttgatcaa catctgaaag ggccaattta atggtctcaa ccaaggcagc
      601 aacttcacta ctttcaaata gatgttctgg agcgatataa agtctattgt gaaccgtttg
      661 gttaaatcca agaatcaaga gattgtgctc aaaggcctga cgcactgcct gcaacaactc
      721 attgctgtga ttgatatcca agtaaatatc cgacagttga tacagctcct gaatcttctg
      781 tggactagca ttctggtaaa ggaccacatt aggatagcga agcatgtcta agagcttaga
      841 agacatctct gtcactgctg cgatacggaa ggtaacatca ggcaaggctt ctacgattgc
      901 ttctacttgc tcaatctgat ctgaattggt caagattaag gcatctcgtc ttaggaaatt
      961 atcacgtttg aactggtaat gataacccaa gtgcacaaac tgatcatggt atttctcgtc
     1021 agttaactct aaagcgcgct cataagtcgc cttatttgga atgatgatct tcttagtacg
     1081 cacattatca ctttccaaaa tcaactgcat attacctgga atggcatcat agagaggttc
     1141 ctgccatacc aagacatccg agccagattt atctggatga tggaaggaaa ccaagaaagg
     1201 agtcgctaga gtattaaaga taagctgact ggtatctatt tccaaatctt gcaaaaagaa
     1261 ggtgataaat tcaactttat ttgcaaagta acgcatggac tgaccaggca aagtcaataa
     1321 gatatcaccc gtcacatggt tttccagtaa aacttgttgg ccattgacat cttggtaaac
     1381 tgtcataatc ggctcgctat ctgcgctata agtggtttta gcaaagcaag caccaaagcg
     1441 attgtagtgg tcaacctgac gtactcgacc ttctaggtct ttccagtcta cctgtttaac
     1501 caagcgagcc tgcattccat caacataatg aatgacagcc ctctcctgcg tcacatcttc
     1561 aatacaagca gactgattat ctcctaaaat ttcccaaaaa tctgaaacgg gaacttggtt
     1621 aaaatagaga ggttttccat cctcgtaacc tagataatag gtaaaaggag aaagcagacc
     1681 atcaggcaga aaaccatctg catcgatgac cactccaagt tgagaaagac cagtagcgac
     1741 tagactttca tgtaaatctc tactttcttg actgtaacta tcataaagtt caatcatgga
     1801 gcacctcctc tactgttttc ttccactttt ctaaaatttc tttggtcaag aagccttctg
     1861 caatttgata agaataggca cgcatagctt ccaaacgatt ttcttgatac aattgacaaa
     1921 tcttagcggc ataagcttgc ttgatttggt cttctacatg gtcagatgaa cttggaatca
     1981 aataaccatt ttgcccatcc tctataaagg tctgattacc ataaggcaca tcaaaaccaa
     2041 ttagaggtag acctgaacca atagcttcca tcaaggtcag accaaatcct tcgctggtag
     2101 aagccgttaa gtagacctca tactggctat aaatctgcga aagttccgca tgccccttga
     2161 gttggatata gtcctctgcc tgatgatttg caataatttc tctaagcaga gaatcttctc
     2221 caccactacc atagatatca aaggttagtt ccggtaactc cttatgagct tcaatcacag
     2281 ctttcacaag ccaatcaatg tgcttttctt tggcaagacg tgaagccgta atcaatgaaa
     2341 atggtttgcg cccttgactt gaatctgtca aggaatcaat actgcctaca ggaatggtaa
     2401 caatctttgg ctgatgctga gtatatttgg caaattgctc ttgtagaact tcattttgtc
     2461 tatcagtaga cacgataaag aagtcaacct tatctgcatt ggtaaactga tagtcataat
     2521 agttattcca aaggatatag tcctcatttg tagcattttc actataatgc tccgcatgaa
     2581 caactaccgc tagatgtgct gtctgtgctt cctcaaacac aacctgtcca atacctgtct
     2641 ccctatccag aataaccaaa tcagacttgt tcaaattcaa agatttcata aaggcacgca
     2701 caaaagcttg ctttccatag aaaatcttat ccttgaaatg ataaacttct tccttccctt
     2761 gattcatcaa aatatcatag actggagtcc cgtcttcatt gtaaaaagtt cgttggtata
     2821 agactgcaac attgtccttg ggagcaaaat actcgctaca ataacgcgta taagaaaagt
     2881 aatccttccg aatcaggttt cccttaaaaa catactcggc atgttgaacc aagtccttgt
     2941 tctcatcaac caaataacag gttacaaact tatcttggtc aaaaaagaat acacgtaaaa
     3001 ccttgccatt tttttctctg tgactttctt caccaccaaa gtaagccaag acatcatcca
     3061 ctgtcacgct agtaggtgca attttgatat ctgtgaaatg attataaagc cagataacct
     3121 gattatcatc aaaaccaata ttggctgtta agtgctgaat attatcggct aaaatcatat
     3181 ctgtaaagat aaacttagag gacagattta atttccgaaa aacaccagca cgataggctt
     3241 gagcgtattc aacaccgcta ctagcccaac caattcctaa atttatattg taaattgtca
     3301 tattttctcc tcttatggaa aatgagtcac gatttcacct tttggagtga cctcaaccag
     3361 ccccatcagg agattacgca ccatatccgc acggatttgt tctttcatag cttcaaagcc
     3421 cgcgtaggct tcttgatagt actctacgat tggatttttc tgactagcag attgaccacc
     3481 gatagccatg gatagctgtt gtagatagtc tacctgctct acccagttgt catcaatggc
     3541 tttaagcagt gaaagtcgta aaaactgttc atataagtca tgttgattaa gtaattcttt
     3601 cttttcagaa agttctttat caatcacctg cttcataaag ctacgaactg cagttttgtc
     3661 agttacatct atataatctg gaacctcttt aacatgaaaa ctaatattgg tcacaataaa
     3721 gtgaaacaat aattcacgac tagcatagtg atcagccgct acctcttctg tatatctctc
     3781 aatgatatcc acaacaacat cctctaagtc acgagaacca tctattagac gatttctctc
     3841 tttatagact atatcccgtt gtatattcat actttcagca tactccagag tctgacgacg
     3901 tgctgaacgt ccagcactat cactggcatg ctgagccttt tcgactagtt tccggtattt
     3961 acgacctttc aatacttccg gttgagtcat atcttgaacc tgatagtctt tgtacttttt
     4021 atgcacccaa gatggaccaa atttcttgat aacatcatcc tctaaggata caaaaaattt
     4081 actcatacca ggatctccct gacgaccaga acggccacga atttgtaggt cgatccgctg
     4141 actttccatc cgctcagtcc caataacaat caagccccca agctctgcga ctcctttacc
     4201 aagcttgata tccgtaccac gtcctgccat agaggtagcc actgtcacag cccccatctg
     4261 acctgactcg gagataatct gagcctcacg cgccgcatta ttagcattta ggacattatg
     4321 ggcaatccct tcacgaaaca agagagacga atagagttga gacatttcaa ctgagcctac
     4381 aaaaacgagt aaaggatttc ccttagcatg gtattgcttg atgtactcca aggatgcata
     4441 cactttttca ggtaaagtga tatatagatt atctggatag tcaatccgtt gtctcggacg
     4501 attggttgga atgcgtacta cagacatatt gtaagtttca ataaactctt tttccgcgac
     4561 cttacctgtc cctgtcatac cagatatctt attaaacatc ttaaaaagac tctgataggt
     4621 gatcgaggcc atagcccgcg tctcaggaga taatttgaca tgttccttgg cttcaatagc
     4681 ctgatggaga cctccttgaa gtttagtcat ttccattaga cgccctgttc ccttatcaac
     4741 cagtaccatc tcatttccac gaatgatata gtccttatct ttagtaaaga gcttatgagc
     4801 tcgaatcgca taaaccaaat gacgagcaaa agacgcatgc tcttccttgt ataaattatc
     4861 aatccctagg aaattctcag cagacttggc ccccttagta gtgagccaaa cctcctcttt
     4921 ctcctcttta aagatataat cctctccttc gaccaaggtt gttacaagtg tatcaatgat
     4981 cgcatagtaa ttagactgaa cacgaggaga acccgcaata atcagaggag tttgtgcact
     5041 atcaagcaag atatcatcaa tttcatcaat aataacatag ttaaacggtc gtaaaaactt
     5101 accttcttca ttcgaggcta ggttatcatt tagataatca aaacctaaac tactattggt
     5161 tgtgtagatg atatccgaag catagataag cttcttttct tcagctttca tttccttctt
     5221 tggatcttcc gtaaatggta caccaatggt caatcctaga aaacgataaa cttggcccat
     5281 ttcctcggca tcacgctttg ataaatactc attaggagtc acaaccatca ctccttctcc
     5341 tgaaaaagcg ttcaaataga caggcatggt agctgtcaag gtcttacctt cccccgtatt
     5401 catctcagca acatttccat agtgcatgac aatagctccc atgacttgaa catcataggg
     5461 gaacatccct aaaatccgct tatctgcttc acgcaccaca gcaaaagctt caaccaaaat
     5521 atcgtctaga ctttctccct cggaaagacg ctgacgaaac tctactgttt tagctactaa
     5581 ttcttgatcc gaaagagagg acatcttgcc tttcagggca ttaatctgct ttaaaatctt
     5641 tttcactttc ctaagctgga aatcttgacc taaacgtcta aacacttctg atctccttga
     5701 tagaaaaatg ataaaaagtt aaggactcaa atccagcact taaaagagaa acttgataag
     5761 tataactatc ttctggataa gtaaatatga aatctttcgc tttttctacc tttttttcta
     5821 aaatatcacc atagcggtca aaaaaagtta tcttggtata aactccattt tcaggctgac
     5881 aatcaaaaac cagtgatagt tgatactcct gctttctttt taataaggga agctgaggtt
     5941 gggttcgtgc agcctggtaa ttgacactag aaaaccaagt ctttagaatc tcaccagatg
     6001 gcaatagagg attatacaga cgaacacttt tgtcaaggta ataagataca gttgttccat
     6061 acatataagt cccaccaact tctccccaat aaatagactg tctctgtgta ataatcatct
     6121 tttctccctt ccaaaaccag tctctaagac cattctataa aagtgcaaaa accaagaaat
     6181 attggtatct gtatcatcat tgtgtcgtcc agcagttcct ttagataaaa tcttcgcacc
     6241 tgtattacac agataagaca ctaattgttc ataggcttgt ggatccattt cttcatcttt
     6301 catataggat aaaccaaagg ttgtctttga aaaatttgct tgtttaaaaa ttttccaaaa
     6361 acgctgatcc aactccgtca tatcttgaga acttgtccca cctgtatgat gaattaaaca
     6421 atcaaaagct aagttagaga cacccggtgc gtccaaacgt ccacgactag ctatagttcc
     6481 taaattagcc aagggcttac caactatgat ggcatggggt tcaaaagaag caccataata
     6541 gagagccgga aacgttccca tagaaagacc tgataaaatc aaatccttat gatcaaagcc
     6601 taaataatca agatagtgag tgatcgtatc ctttactttt ccctctagct catccgttcc
     6661 gagataaaaa gctcccccct ctaaacgtgg atcagaaaat aaaatgaagg gacatccaag
     6721 agttttcatc ataaagtaac cctcgaatcc ttctgcaggt cgataacctg caaaatagac
     6781 agtcaaaggc ggtttaaaat cacctggatg gaagaaatag tttatttcat cacgcttgga
     6841 atcatgtagg atatttccac caagtacaaa cttaccaaat tgttttcgac tccatcgttg
     6901 gtgaagatta cctagtttga tagttccacg acctcttgct tcaacagata tcgagagaaa
     6961 gtagtctccg tctcgactat ccataaaaag tgcttgctcc aagtcttttt cagatactat
     7021 tacttcttca aagaaatcat caacagaccc actccacatt ttacggatta ctaaacgaaa
     7081 gtcacaattg ccttccttct catattcaag ccacaactca atagggagtg ttttttgcac
     7141 cataatatta taagcccaat aggcaagctg ggcaaaatct tgcccaaact caccttccaa
     7201 actgacataa tccaatcctt gataagaaat agctcctgta aaatttggat tgacttgtat
     7261 cgtcggagga aagagtttat caccataccc accgccaaaa agagatttac ttaaatcatt
     7321 gattagttgt tggggactta aaaaatcaat tgcttgtgca catcgttttt ttagaaaatc
     7381 cacaacctct ctatcattta tttgtagatt ctggttatag aacaaagtat aaggttcaaa
     7441 atattttaca taggaaaata agtctctgga atactgacca tcttctatta aaactgcatg
     7501 aaaacggtta atatcctctt tttcgattat ttcacgaagg gtttttgatg agtttgggta
     7561 aaaataatac caactcatat tttcaggtat gtcatagtga ttttcccaat tggcaagtcc
     7621 tatctgtaaa atattaagtt cctttgacat ttttaacctc ctctatccat ttctctaatt
     7681 ttccaagaaa ttgttgtcct gtgtgctcct taatcttatc aattgaatat atcaaggact
     7741 cattccactc cttcaaccta tctgtgtaat aatgtgcagc cttagaaaat tctgtcacat
     7801 ctgtcagcaa ataaccattt tttaaatgtt ccacatagac ggtttcaact aggttgattt
     7861 gaggaattcc tgcactaatc ccagcaatct gtgtgtagag atgaggatgt ctatttaaat
     7921 ccacaatcaa acggacaaat tctagtgttt ttatcaaatc taactcatca ttcaagttaa
     7981 caaactgtaa gcgtaagtcc tggtgttgat tttcttccag gggattttct gcatcaccat
     8041 aatcaatcgc ctttcccaga ttttcggatt gaatgttttc ttggatgaaa gactcaacaa
     8101 tcccttcaac ctcattcatt tgctcctgac tagcagcaaa ggctccaaaa atcacctcag
     8161 tatccttatt ttcagcgaca aaggacaaga cttgaagcag agcttgatta tcaatcccct
     8221 gctcaaaatc cagttgaaaa taaatgatgg attctttctt agtttggctt cgtcccaatc
     8281 gtaatcgggt gtcaaaagat gataaatgat aacattgatg cattcgttca ggatacaact
     8341 cctgcaacaa tcgtaaacta tcctctctat ccaccaaaat caaatccgat ttttcaaaag
     8401 ttacatctaa atccctaaag gtatcttgag gattacgtcc aatgaacaga ctcaacagtt
     8461 taggattagt actaggtaaa cgatctagta ccaactggtc atgatgagaa tgagaaggaa
     8521 gcataaaaat atcctgatcc ttcacatacg tttgcagata attctcaaaa aattctgcca
     8581 ccagatctcc catattttga taggtaagca ttttaaagcg ataacccaaa attggattga
     8641 cttctacctg tcctccttct tttaaacgct ctctaaattg ccaaactccc ttaggattca
     8701 aatattcttg ataggctgct tgcccatctt caaaaaaaat aatactcgat ataaaccctc
     8761 gatcatccat gatatggtgc atatgtattt gaccatttgg ttcaaaatac tgtatatcac
     8821 tgatgaagcc ttcaactcca tgttcaacct tagcaaattt cttcccattt ttttgaacga
     8881 taatcgtaaa gggactataa ataaattcac agtcatcatc ccactcaata tctctaatgc
     8941 ttaaaacttg ggtatggaga ttatgaaaat cttgcataac atcaaaaacg gaataagtat
     9001 ccgtttctaa cacaccatgt ctatataaaa aataacgtag atgaggctgg taagctaata
     9061 ctaatagacg agaatctata tcttgctctt gaaagagccg gatctggtga aaggtatcat
     9121 caaactctag acgaaaatga gaaaaatacc atggagtgat atctgcatgc catgttcttt
     9181 ctgacccata ccaagctgga ataaaataat acataaaagt ctcctaaatc aaaggtttat
     9241 aggtctcatt tagccttaaa gccttgacct cgtctctaat cgtaaaaatc atgcctccaa
     9301 acatcataaa caaacctgga atcattgcca atcgtaataa cttttcatca aacaaaacaa
     9361 aaagcatggg accacctgcc ataaccacat taaagagacc acctatgact gagaaacgaa
     9421 ggaccaatcg attaataaat cgactagtat ccgcacctgg ataaatacca taaatgtatt
     9481 ctccagattt tttcatacgg tctgcaatct cttctccatt catcgtaaca aaagcaaaaa
     9541 tgatactaaa taaaaataag accgaaatat aaacatagac ccacaaaggc tttccaatca
     9601 taaattcctt tgataaagcc gctaaccctg aatgattagg gaaaataaat cccaacaaga
     9661 tgaacaaata agctggtaca ctaagaaaac tcatcacata catataaggc atcccacctg
     9721 caggattcaa catgatttcg agataaaaat agcgtttaaa tcgattgtgt aagccaattt
     9781 tattaacagg aaccaaatag cgagctcgat acataagggc aagtaaataa gaaaagacaa
     9841 gggttaataa agcaagtaac acaataatcc ctgttggaat gtgtactgtc tgaaatgttt
     9901 ccaaaacatc ctgaggaata tttaaaacca tactggatag gagaatgact atagaacctc
     9961 caatccccat actcgcattt aaatctgaca accaaacaag aaaaaatgtt cctgctatca
    10021 gcaatattgt attcattaga acaaccaata ttgcagaata ggaggattgt actggcagtc
    10081 tcaagctaac tgccaatgat tgaatgacag ctatcatcaa ggtcaggtac attttacggc
    10141 gatcttgtat ttctatagac gtagatgtta aacccaaccg tttagaaaaa gaaaacatct
    10201 gccataaaat catagctgac atccaagggg acaatccaac agaaaaaatt gacaaacttc
    10261 ttagatttcc acctgttagg gcggctgaga aggctagata ggctgttgaa cctcctaaaa
    10321 aatctttagt atttaggtca acaaaaggga gagtaatacg acttcccaat acatagataa
    10381 acagtagaaa taaggtaaat agtccttttt ttactgctat tgacgaataa atcttagtca
    10441 taaaacactc ccctcacctt cattgtttgt gatatggtaa ataacaattc ttttatttca
    10501 acttttacca tttctttgtt ctgcatcaaa aaacaacttt aatctgaaac aaatatcttt
    10561 atattcctca gtatctgtta attgatggta ttctaaagtt tgcttataat cttttaaaag
    10621 attgacaaat cgaatacgca gaacggagac atccaaacca gccaagacac aatccgatat
    10681 tttcttagaa aacacttcaa caatatctct tgcccaggaa agatcaaatt ccgttctcat
    10741 gatgcttcct gaacgtcttc tatacagata gagattatcg tttataaaga cgattttaga
    10801 ggctaaaaga taaaagcgat gcatagttgc ttcatcatca aagcggcgac catttgaaaa
    10861 gtgaacttca ttgaataatt cttttttaat caacttaaat gtcggcaata taaaggccga
    10921 gctattgaat ttccaatctc ctgcttgacg gttcataatt tcttgtatag ctaattcttc
    10981 gacgcaaaaa tcttgccctg ttacataaaa ataaaaattc ccgtcacttt cgtcataatt
    11041 atagtaattc cctataacaa catctgcatc cttttcttgg acttgctgat aaagagtttc
    11101 aatcatattt tcttttacga tatcatctga atcaacaatc gtgatgtact ctcccttagc
    11161 tacactaatc cccatatttt tagcttgtgc tgcccccccc ccttattttc ttgatgcaat
    11221 actcgcacac gattatcttg catcataaaa gcatcacaaa tttccccaga attatccgta
    11281 gaaccgtcat caactaaaat aatttctata ttttgatagg tttgcttcag aatatgctcg
    11341 acacactcca ctaaatattt tcccgtattg tatatcggta ccacaacact aattaattca
    11401 ctcattactc tatctctata ttcttcattc tatccactaa atcatctggt ctctccacag
    11461 aaaaaatacc gtcatagagt ccatcatcca tacttttacg tgtgatatca aaagcgaaga
    11521 ttttcttgcc actttcttgt gcccttgtaa ctacattaaa aacctctcca cctgtattaa
    11581 tatctaaata aactatagaa tcgtccaata gccaatcaat tctactatgt aatacatttt
    11641 gatatactgt tacattcgta tactgtgata gagaggtcag ctcctcagaa caatcacacg
    11701 gtgcagccaa atgaaaatgc caatcaggta accgttgtac taaatactct aaatgtttta
    11761 tatctgcact ccatgtcaca agcatcactt gttttttaga ctgattgctt ctttcaaaat
    11821 aatttaaatc ggaacgttga aaagcaatct ctgaccaatc caaatctcta taaacccacc
    11881 ataattcacg tagtctagat atactatatg tattccaagg tttatcatga ctagcatagt
    11941 gaacaattgt aggtggattg tcatctagac gttcacattc ttgagcaagg tgataaatat
    12001 caacacccac catataatta tatgttttat ctaaggctag ccaattatcc tcaaaataaa
    12061 tatttaaaat actctgatcc ccaagatgaa caacttgatt ttgctcggcc gctaattcca
    12121 ataaactatt gacaatagag tgttctttcc attttgcaac atccattagt aacataccag
    12181 cattaaatcc agattttcgt ccttcatagg catagacatc atcaacagca ccaattgaat
    12241 atcctttgag atctatctca aacaaagtag ctagttcccc agttacaatg atatcggaat
    12301 ccagatacaa taccctatca gattcaacca cttctgtcgc aaaaaatcta aagtaagaag
    12361 cataatttat atgaggtcct gttttatagc tttcaaaaag ttctttatca atgtgtacac
    12421 tgcgaattgt agaattcaca acttccattt ttctccccaa taatttaaac cattcaggag
    12481 ctatatcact gttgagaata taaaaatcaa catctctatt gtgatacaat acagacttta
    12541 tagtcgtctc taaaggaata agataggcat tatctgccgc taatactatt gattttctca
    12601 tactgttctc tacctcatgt aagatctgat tgctgcaacc atttttttcg gaatggagct
    12661 tggatagata ccttcatatg aaatctcaga aagattttca gagcaagtat tgtcgaaagc
    12721 tatcattggt tttttgtact taagcacaaa ctcatatgca tcttctaatt ttcgatcatg
    12781 attcaaatcc aaatacaaat ctgaagctat tactatttgt tccaataaca ttggtaagga
    12841 atttggatat acggtaacat taggaaaacg cgttaaagct agtagttcat ttgccatatt
    12901 tgtataagca acaatatgaa aatgaacttc tggcaattgc tccgccaaat aatcgatttt
    12961 ctcgacacac caagaattcg ttaaattaac acattgtaac ttaaatattt gtgatttact
    13021 aggatacttc acactctttg aaaaccattc atttaaaata acagaccaat ccatcaaaga
    13081 gtattcccac caaacttctc ttagacgtcc aacagaaaat tgattccaag gcttatcctg
    13141 agaaatatag tgtaaaatta gtggcagtgg ttcgagcgga atatcaaaaa tgaattgatg
    13201 tttaaaggct gccgccccat aatcatatcc tatttgaaaa ttatattgat cttcaaggga
    13261 actatattga tctttaaaca acatattcaa aattgactgg tctccttctt ccacattctc
    13321 atgttctttt tctgttaagt caatcaattt ttgtcgaata gtttcagatc cccatttttt
    13381 gttgttaatc aagagaacac cagcattgaa gccgactcct gctccaaagc aagaacgagc
    13441 tgctgccaaa taattttcac ctaagtctaa ttcaaacaaa tcggtcaaat caccagtcac
    13501 aatcaaatca ctatctagat agagaacttt atcttctgtt acaaaatctg gaataaaata
    13561 gcgtgcaaat gtcatatgat tgatatgagg taatttatta gaccaattca tttgaaactg
    13621 tgagccaatt aacttgcagt caatcaagtc gccccccatc tcttgtaaat atattcttat
    13681 ttgactaaac cattcctgag gaatgtcctg atttagcaga taaattttca aatgactatt
    13741 gtgtctacag agtgacttca tcgccgtttc gatttgtcga atataagcgt aatcacctgc
    13801 aaatgctaca gcgcgtttgg tatttctcat tttaaatcct tatcatttat tttaaatagt
    13861 ctagtttaat attcgtgcaa caatctgact gttataggtt tcatcatcaa taaattcaat
    13921 atcttgatca aaattatact ctgcagtatg tttatgagaa aatttaacct tagttttagc
    13981 tcccattttc atccattcat actctgagtc aatatggcct acatccaaaa cttgatagcc
    14041 catctggcat agattataac tcagaacttt tgctgtagga cccagcatac ataaaatcaa
    14101 gcgaccacca gcatactttt caatttcttg ttcaatttca tgaactctag aaaaggcact
    14161 atgagaagga cagataattc gcttaataga atttgcctca tcgaataaat catttccgac
    14221 acctgaacga gaagtcgcac cttcgactat cagtaagtca cggttttccc aaatgctttt
    14281 caatttttca aattgagctt tagcttgact cttgtcttca aaatcgatat aagggcgaga
    14341 tacaaaggtt gagccatacc atgaatcact gcataactct ctgtaaaaat ccatataatg
    14401 atccatgtga tctttccaaa atggaatcgc ccacgatgta aacctaaaac gatctgtaaa
    14461 agcatcagga aggcacacta ctaaatcttc tcgactttct tggccgataa tgtccctcat
    14521 gattgaaacc aactcttcat cataatcctg gtagggaatt gaatgccctg caagcatatt
    14581 gatttcccca tctccaaaac gaactaaaga agagttgtgt tcaataatat aatccaaggt
    14641 ttcatctatc cctttcacct ggattatatc aagcggtttt tctagcatat ccttagttat
    14701 caatttttgc aaatcagcca acattcgttc aggatgttca cttgaataca acctttggcc
    14761 attattccca ttttgagatt tataaaaggc tagtataggt ttagatagat gagccatttc
    14821 tgcaacgata tcggatgttg aagtcagtag gttgatatct aaataagcat ccatcttctc
    14881 cttcaactcg tctagcttat ctctagaaat tgccggatag agctgaatat taggataacg
    14941 attagacaaa tcatataatt ttggccccat agctgtccaa gcagcaactt gaaaaacaac
    15001 atttggcaac tgtccagcca agtaatccaa ttgttcgata tcctgagaag ccgtaaacgt
    15061 taaacaagtt aaacttggaa gttgtcgaaa acaggtaaga tgtccgtttt tcttaaaatc
    15121 tttaatcgta tcaatcataa aggaaacttt cccattttcg aaaacgattt gtcctaactc
    15181 tccgtgctga gtcgattgaa aagctagtag cgttttttct tgttcttgca aagattttaa
    15241 aaagttctca tctgcactac catgattgat atccaaatat agatttgttg actttttcag
    15301 cttatctaat acaggaggaa caatttgtgg atgcaatctc acattatcat acacagctaa
    15361 ttttatcagc ttatcaccca tatccgtcca agcagcaata tgaaatacca catctggtaa
    15421 agctgtgact aactcttcta ttccttctaa atcttgggaa tttgtcaaag ttaaacaaga
    15481 aaattcctta tctaaagacg atgtcagttc aaactcccct atatgatgtt gtaaaatttt
    15541 agtccattcc aaatcatgaa attcccacca taaatcacga taacgattgg ctatcaaggt
    15601 ggtccagggt tttctgtaag acacaaaatg aataatacta ggaggtacta actcattaaa
    15661 ataattttcc catttattat aaaaagcagt tacatcatga cccacttgaa ggttaaattc
    15721 tttatccagc tctaaccaat catcttgtaa gacctgatta aaaatcgttt gatcaccatt
    15781 aaaatgctcg aaacggcctt cttcaacttc cttcattgta acaatgctct gttcaattag
    15841 tcgttctttt aatttctctt gacgccattt tttattgttg attaataaaa cacctgcatt
    15901 aaatgtaatt ccatctgtat ctttaactgc tgcgagtgat ttttcttcta aacaaatact
    15961 aaataatttc tctaaagaag tatttacaat caaatcactg tctaaatata aaaccttatc
    16021 ttcttggata taatctgcaa taaaatatct agcataggta atgctactaa tgtgatcttg
    16081 cttttcccaa tcttgaaaca cagtttgttc aggtagcttc acatcgataa tctcactacc
    16141 taacatgcga gctatttttc gtggtttgcg aaaccaatct ggcataatat cttgatttaa
    16201 aatataaatc ttaacatctc gattgtggta taaaatagat tttatggttg tttctaactg
    16261 cctgatgtaa ttacgatcac cagctaatac aattgtttta atcatcttgt tctcttattc
    16321 aactcccaaa tggtaaattg catctaccaa taatttttta gtaaaataac cttcttttag
    16381 caaataacta aaggttttta tccgattcgt catttcttga tattcttgta gattcatttt
    16441 atcaactatc gcatgaactt cttctagact attcgccaca aagcctaagc cttgatctac
    16501 tataaattta gcagttgaca agctacttgg tacaatgact gggatgcccg ctgtcagata
    16561 ggtactcacc ttatgagata tatttaaggt ataatattgg ttgctttctc catcgttttg
    16621 ataagttccc cagacaaggc caaatccacc ctttgataat tctagcaaca attcctcatc
    16681 ttttttccat ccttcgatgc tgagatttct agcactagaa ctagcttccc ctttatttga
    16741 aaatactctc aaaggcgtat cttgagacca attttgtaag tctggaaaac gctctaaact
    16801 tccagcaaaa aaaagttctt ttttaaaagc aggggtgtat aaggataaat catgaggatg
    16861 atcccacatc ccttgaacaa gaatcttctt agtcgtcaat ccttcttcca tcaggcgtgt
    16921 tttcattctc tctgacggca ctatcaaaac atctgataga ttatacatgt acatatagtc
    16981 tttcatgaga taatagttac tatcaaacat gaggggaaca acatcatgga taaagcaaat
    17041 aattttcacc tgcatatcct tcaacttatc aaacaagaga cgatcaaatt caaagccatt
    17101 ccaggttgga gactgaaaga ctaaaatatc cccaatagaa atactggcca taataccatc
    17161 cagacgctta ttcatttcag aaggactatc tgaagcaatg ttgtaaaaat aaataccaac
    17221 ctctctaaat cccagttgac tagctatctt ttgaacagca ttttgagcta agataaccgt
    17281 actatcacca gccatgccgt ataaatttgt taaatgtagt ttcatattac gctatctcat
    17341 tttttattac tatctacgga agtaaagaca atttttccat cattcttcta taaatctctg
    17401 tgaactgcat attttgttct tctaattttg ctatcactct ttgtaatcta tttttataaa
    17461 tcaaaatatg attggtcaag tcatagtctg aacttgctaa aatagctatc ttttcttccc
    17521 tagccccgat ttcatcatac atacgctttt cactccatgt attcgataaa gtatcagata
    17581 aaccaacacg gtaccagtaa acacaacgat tcaaatagac tatcctcgaa gctcttagaa
    17641 gtaccttcca tgtccagtaa gtatcctctg caatttttcc tataggaaat ggtaaatctt
    17701 gtagtaactc tctcttgaat aacttcaaga cagctacagt ccagttccca tttctgactt
    17761 cttcgacacc ttccctatcc atgattgctt taccttctat cacttccaga gaatcatctg
    17821 gatccgtaac ataagtcata tacacatagc gtgtttcatc ataagaatta taacgcccga
    17881 tactaatatc tgcgttttcc tttttcaaag caccatataa tcggtctaaa gcatcatgtt
    17941 ccaaccaatc atcagagtct acaaaagtaa tgtacgcccc ccccgaacat tcaataccta
    18001 ggttacgagc tgatgaaaga ccgccgtttg ctttctcaaa atatttgaaa cgagaatctt
    18061 tctctacaaa ttcttcacat attttggatg aatgatctgg agagccatca ttaaccatga
    18121 tacattcaaa attcggataa gtttgttttt gaatactatc caaacactgt cgcaaataat
    18181 tctcaacgtt gtagattggg actacaatac taattagttc attttccata tactaacttt
    18241 cctagacttg atttattcta tttccctctt aatctcttct ctttacaaca agatttccat
    18301 tattataaac tacactgttt gatggaatat cattgcgtat gagacaacct gctccaataa
    18361 tggtattatc cccaatcgtc actcctttta aaatcgtgac attacttcct atccagcagt
    18421 ctctgccaac tctaatcggc gctgtagtcc actgccactt ttcaatcttt tcagcagtat
    18481 aaatatggtc gtgatcataa aaacgaacac cctctcccat catggtacca gctccgattt
    18541 caattctttc catacagtta aaactacaag agttgttgat aaaaacacca tcatgcagta
    18601 ttaacttacc agaagagaca tgaaaatttt caaaatttct gcagatgaca tttgctccta
    18661 caatgaagtg agccttatca tcgattttaa agacattcca agtcccatct tggatgattt
    18721 tttcttgatg attggtgcct actctgactt cattcagata gtctctcact ctagaaacca
    18781 atttgtctgg tgtacttgat agaaaaactt cctgcccctg atttccatgg actgtattgt
    18841 caaaagcaaa gacagatttt ccagcctgta caaacttgga gacaatagca tctacttctt
    18901 gaaagtgatt gatatctagt aaaaagtcac aggtatcgac tagaaagttt agctgttctt
    18961 catttgtaat cgttcggaga cggacattat agttcgtttc taagctacgg attttctcgg
    19021 aaaattgaac aggtgctgca atataaaaac agatatttgg caaggattca aggagataat
    19081 ccaactcttc aatctgatca ctaacagtca aaatcagagc atctcctact aaatgatttt
    19141 tcttcatttg cttatttctc tcaatttctc aatcattgct tgaacttggt caacagcata
    19201 tacctcctga cctgcttcat aggttttggt attttcaaag gacaaaatag tttttccaag
    19261 tcttgcaaat tgattgataa tttcctctgt tttttcacca tgattaatat ctaaaagtac
    19321 ttgactggtt tctaccaatt cattatcgac atctaccaaa tagtgaattc cgttaaatat
    19381 agtcacgttt ggataaattg tcatctgagc caatcgatca ctaactatta ctctagctgc
    19441 tatcttaaac tgaatatcag gcaaggattg aaccaatgtc tcaatttgtt caatatggtc
    19501 tgaggcagta tagattagac aagtgaaagg ttcctttatt ggatagatgt gagatctttg
    19561 taatggatgt aaatgatggt tttgtcccaa ttctgtccat tcaagcccat gatagtacca
    19621 ccaaacttca cgataggttt gggccgccaa atctttccac ggtttccgat gagaaagata
    19681 gtgaataata gcaggataat cctgaccctc aggcaattga taatcagcaa actgtttatg
    19741 aatgacaata tgattataat caaagtccaa ttccaaccat ttatgttcaa aaagcatatt
    19801 caagatgctc tgatctgcct gatccacctt atcatgccat tcattggtta catcaattaa
    19861 tttttgggtc atattctctt ttttccaaaa agcattgttt accaagagaa caccggcatt
    19921 aaagatttct tgaccaaaat aagctctgcc cccaaaatct ctaacagcag ccaaaggata
    19981 atcttgtaag tctgtagcaa acaaatcatc aagatttttc gttacaagta aatcacagtc
    20041 caagtagagg gccttatctt cttgcacgaa atcagctatg aaatagcgta aaaaaactgt
    20101 gtaactaata tccgttttat aacatgaaat ttgctcagaa gttacccgac aattaataat
    20161 ttctgagtca aacttctcta agcgcttatt taattgctta atccattcat ttggaaaatc
    20221 gctatgaatc agataaaaac gaatcgaacg attatgataa caaatagact tgattgtcgt
    20281 taaaacttgg tctacatagc catagtttgc tgctaggaca atggctttct tttcacttga
    20341 ttcttgtacc gttaactgat ttaaaagtct ttttttcatt tcaaactctt tatatattgc
    20401 tgtgtcagat aaaccactag cttgaccgtt ggcgagactg acttccaaca tctgacgata
    20461 aactgccaag tgtctctcta gaggatatcc catattagct agtagcgtaa tacgttcaga
    20521 catagcatca actaaggcgt gcatccactt ttctgtccaa actcttgata gactaccttt
    20581 tctaatccga taagcataaa gacctttatt taaataaatt accttttccg ataataaata
    20641 taccttttga ttgaggtaac catcttctcc taatttaccc atgtcaaagc gcaactgctc
    20701 aaacaatctt gccttataga gtttaccaca agcagatatc aaagcaaaac tcttcatttc
    20761 ttgagtttca taacaagttc tcaaagatgg aaacattgtc atatacctta tcataatagg
    20821 agtctcctga tatatgaaag tagaacattc cttcactttc gttgaaagaa tagtaattcc
    20881 caactgcaat atcagcttga tattctgtta tttttttata tagggtttct acataatcta
    20941 actcaatcca atcatctgaa tccacaaagg tcacataatt tccggacata ttattcagac
    21001 cggtgtttcg tgcggcagaa ataccagcat tttcttgttc tatatagaga attcggtgat
    21061 ccatttctga aaattcttta caaatttcac ctgaagcatc cgtagaacca tcattaacga
    21121 caacaatctc aatattttta tatgtttgag aaataatact atctagacat ttcctcagat
    21181 agttttctac attgtatact ggtacaatga ccgtaatttt atcatccact agcaattttc
    21241 tccttgattt atgtgcgcga gttagtttag cctaacttta caggttgtct tactcttctt
    21301 catcacgttt acggcgtttc gcaaccaatc caatacctgt aacagctgct agaactccaa
    21361 gtaacacaga tccaattgac gactcagtac ctgtattagg caattctttt tgggatttac
    21421 ctgtcgatcc agaagcattt ccaacttgtg agttcgaatg gtttgctgaa tttgaaactg
    21481 atgtacttgc tgaggctgac gcactcgttg atgctgactc agacgcactg gttgatgccg
    21541 attcagacgc tgaggtgctt gctgatgctg aggcactggt tgacgctgaa gctgaggcgc
    21601 tagtacttgc tgaagccgag gcactggttg atgctgaggc tgaggcgcta gtacttgctg
    21661 aagctgaggc acttgtcgat gctgaggccg aagcactggt acttgctgaa gccgaagcac
    21721 tcgttgatgc tgaggctgaa gcactggttg acgctgaagc tgaggcactc gttgaggctg
    21781 aagccgaagc actcgttgat gcggaagctg acgcactggt acttgctgaa gctgaggcgc
    21841 ttgtgcttgc tgaggccgag gcacttgttg aggccgattc agaagctgag gtactcgttg
    21901 atgcggattc agatgctgag gtgcttgctg aggccgaggc gctggttgat gcagaagctg
    21961 aggcactggt tgacgctgaa gctgaggcac tggttgaggc cgattcagaa gctgaggtac
    22021 tggtgcttgc tgaagctgag gcactggttg aggccgattc agaagctgag gtactggtgc
    22081 ttgctgaagc tgatgcactt gttgatgctg aggctgaggc gctggttgat gccgattcag
    22141 acgctgagat acttgctgag gctgaggcgc ttgtacttgc tgaagtcgac gcactggttg
    22201 aggccgattc agatgctgac gtacttgcac ttgttgacgc cgatgtgctt gcacttgtcg
    22261 atgctgacgt gcttgccgat gtacttgtgc ttgttgatgc tgacgtgctt gctgaagccg
    22321 aagcactggt gcttgctgaa gccgatgcac ttgtacttgc tgaggttgat gccgattcag
    22381 acgcactcgt tgatgcggaa gctgacgcac tggttgacgc tgaggctgac gcactcgttg
    22441 atgcggaagc cgaggcactg gtgcttgctg attcagatgc acttgtcgat gctgaagccg
    22501 aggcacttgt cgacgctgag gctgaggcac tggtacttgc ggaagctgaa gcgcttgtcg
    22561 atgctgaggc tgatgcactg gttgaggccg attcagacgc tgaggtactt gctgaagctg
    22621 atgcactggt acttgctgag gctgaggcac tggtgcttgc tgaagctgat gcactggttg
    22681 atgctgaggc tgaagcactt gtacttgctg aggctgaggc gcttgttgat gcggatgctg
    22741 acgcactggt gcttgctgaa gctgatgcac tcgttgatgc ggaggctgac gcactggttg
    22801 atgcagattc agacgctgag atacttgctg aggctgaggc actcgttgac gctgacgtac
    22861 ttgcgctggt tgatgcggaa gctgaggcgc tt
//
LOCUS       DAVBCW010000020        22209 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVBCW010000020 DAVBCW010000000
VERSION     DAVBCW010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22209
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.020"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(70..2028)
                     /gene="ftsH"
                     /locus_tag="VQ514_000693"
     CDS             complement(70..2028)
                     /gene="ftsH"
                     /locus_tag="VQ514_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET2499488.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEETGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMLGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDEK"
     gene            complement(2044..2586)
                     /gene="hpt"
                     /locus_tag="VQ514_000694"
     CDS             complement(2044..2586)
                     /gene="hpt"
                     /locus_tag="VQ514_000694"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HET2499489.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            complement(2590..3867)
                     /gene="tilS"
                     /locus_tag="VQ514_000695"
     CDS             complement(2590..3867)
                     /gene="tilS"
                     /locus_tag="VQ514_000695"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HET2499490.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETIFMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ELIQQIPVSRETSIHIRHRKTGDVLIKNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            complement(3864..5132)
                     /locus_tag="VQ514_000696"
     CDS             complement(3864..5132)
                     /locus_tag="VQ514_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HET2499491.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKVWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            complement(5252..5620)
                     /locus_tag="VQ514_000697"
     CDS             complement(5252..5620)
                     /locus_tag="VQ514_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HET2499492.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            complement(5613..5879)
                     /locus_tag="VQ514_000698"
     CDS             complement(5613..5879)
                     /locus_tag="VQ514_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HET2499493.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            complement(5937..9446)
                     /gene="mfd"
                     /locus_tag="VQ514_000699"
     CDS             complement(5937..9446)
                     /gene="mfd"
                     /locus_tag="VQ514_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HET2499494.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYAQHAFIHQLKENG
                     YQKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERELAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPIQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTSICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNIDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKESKEKNSI"
     gene            complement(9447..10016)
                     /locus_tag="VQ514_000700"
     CDS             complement(9447..10016)
                     /locus_tag="VQ514_000700"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HET2499495.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVKYYLQEKNFEKTMQRYNG"
     gene            complement(10087..11202)
                     /gene="ychF"
                     /locus_tag="VQ514_000701"
     CDS             complement(10087..11202)
                     /gene="ychF"
                     /locus_tag="VQ514_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HET2499496.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIIEFRFNV"
     gene            complement(11286..11480)
                     /locus_tag="VQ514_000702"
     CDS             complement(11286..11480)
                     /locus_tag="VQ514_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HET2499497.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            complement(11545..12681)
                     /gene="dnaN"
                     /locus_tag="VQ514_000703"
     CDS             complement(11545..12681)
                     /gene="dnaN"
                     /locus_tag="VQ514_000703"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HET2499498.1"
                     /translation="MIHFSINKNLFLQALNTTKRAISSKNAIPILSTVKIDVTNEGIT
                     LIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQN
                     QIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRP
                     ILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDD
                     IETVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSME
                     RARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYL
                     IDSLKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN"
     gene            complement(12840..14201)
                     /gene="dnaA"
                     /locus_tag="VQ514_000704"
     CDS             complement(12840..14201)
                     /gene="dnaA"
                     /locus_tag="VQ514_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HET2499499.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYDYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVSMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
     gene            complement(14414..15172)
                     /locus_tag="VQ514_000705"
     CDS             complement(14414..15172)
                     /locus_tag="VQ514_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HET2499500.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            complement(15230..16411)
                     /locus_tag="VQ514_000706"
     CDS             complement(15230..16411)
                     /locus_tag="VQ514_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET2499501.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVIVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            16594..17073
                     /gene="rlmH"
                     /locus_tag="VQ514_000707"
     CDS             16594..17073
                     /gene="rlmH"
                     /locus_tag="VQ514_000707"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HET2499502.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            17117..17190
                     /locus_tag="VQ514_000708"
     tRNA            17117..17190
                     /locus_tag="VQ514_000708"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17151..17153,aa:Arg,seq:tct)
     gene            17355..17480
                     /gene="comC"
                     /locus_tag="VQ514_000709"
     CDS             17355..17480
                     /gene="comC"
                     /locus_tag="VQ514_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HET2499503.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRISRIILDFLFLRKK"
     gene            17501..18815
                     /locus_tag="VQ514_000710"
                     /pseudo
     CDS             17501..18815
                     /locus_tag="VQ514_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            18812..19564
                     /locus_tag="VQ514_000711"
     CDS             18812..19564
                     /locus_tag="VQ514_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2499504.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            19616..19687
                     /locus_tag="VQ514_000712"
     tRNA            19616..19687
                     /locus_tag="VQ514_000712"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19649..19651,aa:Glu,seq:ttc)
     gene            19693..19766
                     /locus_tag="VQ514_000713"
     tRNA            19693..19766
                     /locus_tag="VQ514_000713"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19727..19729,aa:Asn,seq:gtt)
     gene            complement(19807..20349)
                     /locus_tag="VQ514_000714"
     CDS             complement(19807..20349)
                     /locus_tag="VQ514_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HET2499505.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            20528..21295
                     /locus_tag="VQ514_000715"
     CDS             20528..21295
                     /locus_tag="VQ514_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HET2499506.1"
                     /translation="MIGISLIPDLYNIIFLSSMWDPYGQLSDLPVAVVNNDKEASYNG
                     NTMAIGKDMVSNLKENKTLDFHFVDEEEGKKGLEDGDYYMVVTLPSDLSEKTTTLSNI
                     QSTAAYQSLTSEQQTEISDSVSQNSTDSIQSAQSIVALVQDLQGSLENLQNQSSNLST
                     LKNQSNQVSPITSTSLIGLSSGLTEIQGDVTSKLVPASQSIASGVNAYTTGVDKVSQG
                     ASQLSEKNATLTGSLDKLVSGSNTLTQKSSRLTAGVG"
     gene            21380..22060
                     /locus_tag="VQ514_000716"
     CDS             21380..22060
                     /locus_tag="VQ514_000716"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HET2499507.1"
                     /translation="MADGSGKLAEGGTKLTSGLEDLQTGLASLGQGLGNASDQLKSVS
                     TESKNAEILSNPLNLSKTDNDQVPVNGIAIAPYMISVALFVAAISTNMIFAKLPSGRH
                     PESRWAWLKSRAEINGIIAVLAGILVYGGVQLIGLTANHEMRIFILIILTSLVFMSMV
                     TTLTTWNSRIGAFFLLILLLLQLASSAGTYPLALTNDFFRSINPWLPMSYSVSGLRQT
                     ISINKSFS"
BASE COUNT         6873 a         4086 c         3623 g         7627 t
ORIGIN      
        1 cattttctcc attctgttcc ctttagctcc taaactgcac aaaaagagag gctccagcct
       61 ctctcagggt tatttttcgt cattcatttt tgactttact tcatcatagg atagtgcatg
      121 agattcctct tctactgctt caggcatctt tcctgtttcg taaagagctt taatttgtgt
      181 actatccaat gtttcgtatt tcaataatgc ttctgcaatt aacttgtgag tttcacgatt
      241 tgactgaata atttcagcag ctttatttcg tgcctcattt aataatgaac gaacctcttc
      301 atcaatttca taagctgttt gttctgaaat tgatttttga ggactctgtg caccaagcat
      361 agcatggttt ccttcatatt gtactgggcc aagtttttca ctcataccgt actctgtaac
      421 cattgcacgt gccatttgtg tcgcttgttc aaagtcgttt gaagctcctg tagtttggac
      481 attaaagata atttcttcag ctacacgtcc acccattaag ccagccaatt gctctttcat
      541 atcttcttta gatagaagca tttgatcctc tttaggaagt gcaatcatgt atccgcctgc
      601 acggccgcgt ggtacaattg taaccttatg gacaacgcga gcattcgaca agactagacc
      661 aacaatggta tgtcctgcct catggtaagc aaccaattct cgttcttttt gtgaaactgt
      721 cttatctttc ttagaaggtc cagcaataac tctatcttct gcttcatcaa tatctgaagc
      781 atcaattatc gatttattgc gacgagcagc aactaaagct gcttcattca agacattctc
      841 taaatcagca ccaacaaagc ctggagtttg ttgagccact aatttcaaat caacatcttc
      901 tgctaaaggc ttgttcttag cgtgaacttt caagattgct tcacgacctt taacatcagg
      961 acggccaacc aatacttttc tatcaaaacg tcctggacgc aaaagggcag gatcaagtac
     1021 atctgaacgg tttgtcgcag cgatgacgat aatcccttca tttccctcaa aaccatccat
     1081 ctcaatcaaa agttggttca aggtttgttc acgttcgtca ttacctccgc cgagaccgac
     1141 tccacgttga cgtccaacag catcaatttc atcgataaag atgatagctg gtgctgcttt
     1201 tttggcatcc tcaaaaagag agcgaacacg actagctcca actccgacaa acatttctac
     1261 aaagtcagaa cctgagatac taaagaatgg aacacctgct tctccagcga ctgccttagc
     1321 aagcaaagtt ttacctgtcc ccggaggtcc ctccaaaaga acacctgctg gaatacgggc
     1381 tccaagtttt gtgaatcgtt ttggatcttt taagaactca acaacttcaa ctagttcttg
     1441 tttttcttcc tcagctccag caacatctga aaatcttact ttaatatctt ctttatttgc
     1501 tgctttagcc ttactacgtc caaaactcat tggattacgg ccattgcctc ctcccatatt
     1561 tcccatcata gagaatagga agaagaatag aattccaaat ggcacaatgg atacgagtag
     1621 attaatccat ataccacttg aactttcatg cttaacagtt acttctgctt tatggtcagt
     1681 agcaagtttt tgcaattctg atacggtagt atctgcagga agaatagtgc tggtaaattt
     1741 ctctacctta gtaacagatg gcgtgaaaaa ctgaatacct gtttcttctt tacttgtttt
     1801 aggattttta tagacaccag aaacttcgat aacactacca tttggttggt aagttaattc
     1861 ttttacatta ccatcggtaa tttcttgtac caactcagta tagttgattt gctgacttcc
     1921 tcctgagtta ttcccagaat agaaatactg gaatcctgtc acaaggaaaa agataaataa
     1981 taaccataga aaaggatttt taattaaacc attattttgt tttttcatta aagattattc
     2041 tttctaattt gaatacactt cctctttcaa tactccaata taaggaagat tacgataatt
     2101 ttctttgtag tctaaaccat aacctactac aaactcattt gggatagtaa agcaggtata
     2161 gtctgcctca atttctacaa cacgtccttc tggtttatcc aacaaggttg caattttaac
     2221 agaagctgct tctctttctt taaacatatc tcgcaaattc ttcaaagttt gacctgtatc
     2281 aatgatatct tctacaaata gaacatgtct tcctttgata tcttgagtca catcttgttt
     2341 aatattgata acaccactac ttgctgttcc accatggtag ctagaaacca tcatgaagtc
     2401 catttcaata tgtgtatcaa tatgtttgac caattcagcc ataaaaggaa tagatccttt
     2461 taaaatccca actaagattg gattttttcc tgcataatct ttagttaatt gagcacctag
     2521 ttttttagct gcttctgtaa tttcatcgtg tgaaacgagg acttttttaa tatcgttttc
     2581 taacattttt tacctatcta ttttttctat ataaagtaca gtgttcatta tatcattttt
     2641 cgttttttta ctcaaattat tggtcgcaat tcccaaaatt gagacaattt caccaaattg
     2701 ctcaataata agagcagagt ttctcttttc catagggatt ttcaaatcaa taaataaacg
     2761 tctgagtttt tttctatgcc catttttaat caaaacatct cctgtttttc gatgacgaat
     2821 gtgtatggat gtttcacgtg aaacaggtat ttgttgaatt aattcacctt ctaatggaag
     2881 tccaaaagaa aataaatatc cttgataagc tacctgattt tgatagtgta acacaagttc
     2941 atcttccttt tcatcagcct gcggactgat tttacaaatc tgaaactgtt ggtactcttt
     3001 tatcaattca tagccatttt taatcggatg acgatactgg cttttagatt ttaaaatctg
     3061 ctgaacttca gcaaactgag cttttgtaag attcaaatct ggaaaacgat tcagataagt
     3121 ttgaagtaaa actctttgtg tagactcaga gtaagaaaat aactgctgta aatcttccac
     3181 attaatattg ttagataatt cagctattgc caaatcataa tctaaaattt cattgccaat
     3241 gcctaagatt gcatccctaa atcgaggatt ttctttttcc aattctggta agtaagaatt
     3301 tcgaatacga tttcgaaaat aatgattctc ctgatttgat gtatcttcaa agtgaaaaat
     3361 tgatggaaag tcttttttct gaaaatgcaa gaagggacga atgatttcta tctctccgac
     3421 tacttgcttc tccttaattc ctgatagata gcgcaagcga gttcctcgaa tcaagcgcat
     3481 aaaaatcgtt tccacctgat catcagcatg gtgggcagtg actaaagctg tcgcacctgt
     3541 ctttttcatg acctcttgaa aaaaatcata acgaaaattt cgtgcacgcg cttctgaaaa
     3601 ttctcctgaa aaattgctga tataaatagg aagctctgct tcagcagcca acttccttaa
     3661 ttccttttct tcccaatctg attcaattct ctgcttatga ttcacatgag ctagaatcaa
     3721 ttcaatctct aactcttttt gataagtaga caatacctta aatagaaaca tggaatctaa
     3781 tccaccagaa agagctagaa ccgccttagc atgctttttg aaatatccct tcttgagaaa
     3841 atgatttaaa aaatctggtt ccctcatttt agaacctcat aaacatcctt ggctatctta
     3901 gaaatcgtat cataatcaga attcttagtg aaaatagaaa gaataaatgg agaatctgca
     3961 tagacaacac ccgtatcatg cttaaattca tccgcatctc caattttatg agctacttta
     4021 acagaaacac ctttggcaat tcgctgacta tcaaaatctg ttttagtcaa agactctagc
     4081 acaaatccat tttgattata aatagcttcc ataaacttcc cggccatctt agaagaaatc
     4141 aatttttctt ttggatccca atcatctccc ataatggcag acatcttgga tttgaatgtg
     4201 gcatcagatt ggtttgaaat gtaatatccc aatagattat gagctacatt atcagattct
     4261 tttgatactt tcgtaattaa atcctttaaa gaatattctt tattatcttc ttttttagga
     4321 agactaccac ttccctctgg tttataagaa cctggaaaat cattgactgc agatacgtat
     4381 tttacagtcg tatctaactg ataaagaccc tcatttattt tttcttgcgt ataatagaga
     4441 taagagagtt tcaaaacgct ggctgcatac atcttttcat cttgattgat accagcttct
     4501 tttccagtag tcagttgctt aacataaata gagaaagaat ctttctgata tttttcagat
     4561 aacatttctt gaactttact catccgatta tcttcttcag aagttgattc tttagctacc
     4621 catccagcct gatcaatatg tagaaattct cttccttcta caaacatggt cttgtcgatt
     4681 gatacttgcg aataagctga taaggatgat ttcacttctt ttaaatcata aggactattg
     4741 tacagtttaa agtcagattc taaccatact ttttttattg ttggagttac ctctgattga
     4801 tcatataaaa atcgtttgtc cgcagctata aattgatgat ttgatagctt aaatactgga
     4861 attccttgtt tatttaagcg ccactcggtt atttgaaaac ttgttttggg agtcaatttc
     4921 ccagattcca ctactaaatc ttcattcgca taaacaggaa cctctccata aaccatggga
     4981 gaacttaatt tttctctaaa ataaatacca aagtcagatt gtgaaaggta ataaatttct
     5041 ttcgaagtat agacgacttc tttttctgtg ctaacgactt ttgaaatggt caaaaaactt
     5101 ggtagcaaca aaataattaa gaatttacgc attcttcctt tccttttctt cttgtaaacg
     5161 caataattga tttttatcag ccaattcttc cagcttttca tctgacaaac tcaaaaattg
     5221 ctctattacg tctaataaat tttccatttt atcacctttg aagcaagtca ggaatcgtat
     5281 aaactttttc cctcgactta gaataatagt acttcgctcg tgtatatttg gcagcataat
     5341 cttcatcttt caacttggta gcaaatgctg tctccttatc cttttcatca ctcaaagttt
     5401 gatactgagt ttgcaagtct gctaattgct gacgtctttg gagtaattgc tgataactct
     5461 gcgctaaatt aaaagttggc aagataaata atagcataat caaaatcaat acccctccca
     5521 taaaacgatt ccgtttttgt cgttctttca tcaggtagcg acgacgttgg tattcatttt
     5581 gaataaaaga attattcaat tgtacaatat ttttagacat tttcttctac ccgtgtttca
     5641 ctgataattt catacattcc tgctgcatct tctttttttg tactatcttt catctctagt
     5701 acttttacaa gcagcaactt attgccaaag cgaatttcaa cttggtcatt aactttcaag
     5761 tccgttgaac ttttggccaa gattccatta accttgattc tacctttatc tgctacttcc
     5821 tttgcgactg tacgacgctt gataattcgc gatactttta aatatttatc taatctcatc
     5881 cttattacct caatttatta ttgtaccatt tttatcttct ttatagaaga aaaatatcaa
     5941 atggaatttt tttccttaga ctcttttatc tctaataaac tttctccaaa aatcagcaga
     6001 ccttctaaaa tttcataatc tttcttattt tggacatcaa atacaagctc cattaatccc
     6061 ttattctcag cgatgcctgc ttttaagttc gttacggata aagctttaaa ataatcttga
     6121 gctaaaaaca gtcgttgagt gactttttca aattgaattg taattttatt atcttttctt
     6181 tccacacgtt gaacaaagac cttgtccaag tatgatttga ccaaaccaat ctctaaaaga
     6241 taggctacta catctgggta ttctccaaaa cggtctatca actcctcttg taactcttca
     6301 taattgacac ggttgtcaat ttgacgaatt ttcttgtaaa tttcaatctt atgtcgttga
     6361 tcagaaatat aagtatcagg aagataggca tcaatttgca aaatcaactc agcattccct
     6421 ttggttcttg tgttagcgtt agcattaccg tttcgtttag caatagcttc ctctaataac
     6481 tgcgaataca attcaaaacc aacagaatca atgaaaccag actgggattt tcctaaaaga
     6541 tttcctgctc cacgaatcga aagatctcgc attgcaatct taaagccaga gcccaattct
     6601 gtaaatcctt taatcgcttc taatctcttt tcagagactt cactgattga tttttctgga
     6661 cgatacatga gataagcata agcaatacga ttactacgac cgactcttcc tcttaactga
     6721 tataaggttg acaagcccat atggtccgca ttttcaataa ataaagtatt agcatttgga
     6781 atgtccaccc ctgtctcaat aatagtagtc gtcaccaaga tatcgtattg tccctcaata
     6841 aagtctaata gagtattttc caactggact tcactcattc gaccatgaac atatccaatc
     6901 gaagcctccg gaatcaactc ctgtaattct gaaaccttct gaacaattgt gtcaactttg
     6961 ttgtaaagat aataaacttg acctccacgc tccatttcac gcaagacagc atcacgaatg
     7021 acactatcat tcttttccaa aacataggtc tgaacaggat agcgattagt cggcggagtt
     7081 tcaataacag ataaatctct gattcccagc atagacatat ggagggtacg agggattggc
     7141 gtagcggtca aggttaggac atccacttgt ttcttcagtt ctttcaaagt ttccttatgc
     7201 ttgacaccaa atcgctgttc ctcatcaata atcatcaagc ccaaatcagc aaacacaaca
     7261 tcttttgaca aaacacgatg tgttccaatc aaaatatcga cttgaccgtt tttcaatttt
     7321 tcaagtgttg cagtctgctc ttttttactt ctaaagcgac tcaacacatc aatattaact
     7381 gcaaaatttt ggaatcgttc cttaaaattc gtatagtgct gttgcgctaa aaccgtcgtc
     7441 ggaactagaa tgacaacctg tttgtgatca ttgactgctt taaaggctgc acgcatagca
     7501 acttcagtct ttccaaaacc aacatcccca actaaaagtc gatccattgg ctgagaagcc
     7561 tgcatatccc tcttgatttc ctcaatacta cgaagttgat catccgtttc aacataaggg
     7621 aaagcatcat caaaggcatc ttgatcatca tcatcagctg agaaagcaaa acccttcaac
     7681 tgactacgtt cagagtagag tttgattaaa tcatcagcta tatcctctac ctggttctta
     7741 accttttgct tggccttttt aaaatgaccg tcatttaatt tattgagttt tggagcttta
     7801 ccatcacttg aaatatattt ggacagtaga tgaatctgtt ccacggggat agaaatttga
     7861 tcaccatttt ggtattggac actgacataa tcgcgatgaa ttcccttgat ttcaatggtt
     7921 tcaattccta gatattgacc aatcccatgg atatgatgga caacatagtc ccctttttca
     7981 agttcattgt aatcttttaa tctctctgca tttgaaacat gttgtcttcg aaaacgacgc
     8041 tttaatttct tttgaaaaat ctcatgttca gttatcaata aaatcttttc atctacaaaa
     8101 tgaaaaccat gtctgagatt accctctatt aaatttacag attctttaca gatacttgtc
     8161 ttatctctag aatccaattt aatctgatat tcctctaaca tatcctccaa tgttttactt
     8221 cccattgaat tgctagactg cagaataatg gtgtaatcca tttttttata tcgttcaatt
     8281 tcttctttta gaaaagaaaa ctgattgaaa aattcctgta taggatattg attgaattga
     8341 taaattttgt caaatttgag atttcctaaa cccttttgaa gattagagaa aaaggtcact
     8401 ggactttgtt ttttatagat ttgttcaata tcagaaaaat actgcatatc agaaaatgct
     8461 ttactattct gtaattcttc tgtaaagtac tgcgctaatt ctctttcaaa gacttcatac
     8521 tgattcatca atttttgata atcatcaaag aatattggag tatctttttc aatataatca
     8581 aagacagtcc atgtcttatc atagcacaaa gataaaaact tccgagaatc tgcatgactt
     8641 tgtttttggt gaaaacttga aagaatttct tctaggtatg atttcaaaat aggtgataaa
     8701 gtttttgaaa tttgtttttc taaagctgac tgtcctcgtt gataatcctt ttctctcaaa
     8761 agcatatcac tagctggaaa gatagtgagt tctgtcttat tttctttcga taattgtgtt
     8821 tctacttcaa atgacctgat accatcaatt tcatcaccaa aaaactcaat tcgacaaggt
     8881 tctaactggg atatttcaaa aatatctaaa atatctcctc gaagactaaa ttcgccctga
     8941 gtttgtactt gagtaacttt ttgatagcca ttttccttta actgatggat aaacgcgtgt
     9001 tgagcatatt cttcaccaac tgagattttt acaatactat ctttgaatgc attgggagac
     9061 ggtaaaatca atcgacttgc tgcgatatta caaactaaaa tccctttctt agatgaatca
     9121 gtcaaaaaac gcaaggcttc aacccgtgaa ataatttttt cctgtgaaga catcaaaaac
     9181 tccaccatag gagcatcatc taccaaaaat ggatagacga gttcctcacc caagatagaa
     9241 ataagatcac taacaagtcc ttctgcttct ccataagttg acgtcaataa cacaatccta
     9301 tcttcttttt ctaaactgct tgcaattgca agagccttag tagatgttga taaaccaagt
     9361 attagttgtc ttttcttatc tgttaaattt tgatgccatt ttttaatctg atcattttct
     9421 gagaataaat ctaataaggt caccatttat ccgttatacc tctgcattgt tttctcaaaa
     9481 tttttctctt gtaaatagta ttttacagaa tcgtcaactt tgtcaacaga ctgtaaaata
     9541 ccgatataat catccctgtc aaacttactc aaaacatgat gaacaactga cataccattt
     9601 ttaggtcttc caattccaat cttaacacgg ttaaagacct gagttcctat atgttgaata
     9661 atagacttga taccattatg accacctgct gagccttttg ctcttaaacg aattttccca
     9721 acttccatgt caagatcatc gtaaatgata agtaaatcgt caatatccaa accatagtaa
     9781 gttaataaag catgaactgc ttttccactt tcattcataa aggtcgttgg tttaaccaga
     9841 taaatttttt ctccatttag gaaaaaggat gctaggtcag cttgaaatat cttatcgtgt
     9901 gtaaaagtga cattctgttt cttcgctagt tgatcaatca acataaaacc aacattgtgt
     9961 tttgtttcaa aatatttatc ccctggattt cccaaaccta caagtaattt ggtcatttat
    10021 ttttcctttc aaaagccaaa agggttggaa tttttttcca acctaattga caccatttat
    10081 taatttttag acattaaagc ggaattctat gatgtcgcca tcttgaacga tatattcttt
    10141 tccttcttca cgcaagcgtc cagcttcttt tacggccttt tcagatccgt atttcactag
    10201 atcttcatat gacatggtta ctgcacgaat aaagcctttt tcaaagtctg agtggataat
    10261 accagctgct tgaggagcct tcataccacg tttgaaagtc caagcgcgaa cttctttttc
    10321 accagctgtg aagtaagttc ccaatccaag caagtggtaa gctgcacgcg tcaacttatc
    10381 tacacctgat tctgtcaaac caatggcttc aagaaactct tttttatctt catcatccaa
    10441 ttcagaaatt tcttcctcag cacgcgcaga aataacgact acttcagcat tttctgtcgc
    10501 tgcaaattca cgaatttgtt tgacatagtc gatagagtca ggttctgaaa ccacatcctc
    10561 gtccacatta gctacataaa gaactggttt agtcgtcaaa aggaaaagac ctttgacaac
    10621 cttttgttcc tcatctgtaa attcaatggt acgagctgat ttcccgtctt ctaggactgg
    10681 tttaatcttt tgaagaacat tgaattctgc tactgattct ttatcttttt gcgtacgtgc
    10741 catcttttct acacgcgcat atcgtttgtt cactgattct aagtcagcaa gaattaattc
    10801 cagattaatt gtatcaatat ctgcaagtgg atctacaaag gcgtcttcac gtccttgctc
    10861 gcgcatcaca ttttcatcat caaaagcacg aactacgtga acaatcgcat ctacttcacg
    10921 aatattggcc aagaatttat tccctagccc ttctcctttt gaagctcctt ttacaatccc
    10981 tgcaatatcc gtaaattcaa atgttgtggg aactgtcttt ttaggagtta tcatttcagt
    11041 tagtttttgt aggcgttcat ctggaacttc caccattcca acatttggat caatcgtcgc
    11101 aaatgggtag tttgctgcct ctgctcctgc ttttgtaatt gcattaaata gtgttgattt
    11161 accaacgttt ggcaaaccaa cgatacctgc tgtcaaagcc atatttctca ttctccgttt
    11221 tcatttcaat ccctaatatt ataacacaaa aagggataaa gtgctaaccc tctaactaag
    11281 ggttctcagt caataatttt attcattttt cgctcaaaat catatcgccc catcatgaca
    11341 acatgctcac aattactgca ttttattttg atatctgctc ctacacgtgt aatttcccaa
    11401 cgattagcct ttttaccagt tgacttgatt gtacaagcgt gtgatttttt catctcaaca
    11461 aaatttccaa cttgatacat actactctcc ttttcttttt cgattatatc ataaaagagg
    11521 cgagccaggc tcaacctctt tcacttaatt tgtacgaact ggtgtaatga gctgcatgaa
    11581 gtcttcgtca gtatctgctg gcacaagagt aaatggacga acagctgaga taaagctaat
    11641 agtcaccttt tcgctattta aagctttaag agaatcaatc aagtaagttg ggttgaaact
    11701 aatggtcaaa tcttcaccag taacctgatc agtatcgatt tcttcgttta ctttaccaac
    11761 ttctggagag tgaacatggg cgctaacaac cccatcctta atttcaagtt tcacagtacc
    11821 attttgagtc gcacttgata aaagacgggc acgctccatt gactggcgta agtttaccac
    11881 attaaaagta atagtagtgt taaagtctgt tggaatcaag cgatctgtat caggatagtt
    11941 tccttctagg agacgagtat agaagctaat attttcgctt ctaaagagga tttggttatt
    12001 ggcaaagaaa atctctacag tttcgatatc atctgtaaat accgctgaaa attcgcgtag
    12061 agaacggcta ggaattacga catcaaaatc atcactattt ttttcaagag tcaatttttt
    12121 ctggcttagg cgatgagagt ctgttgcaac tgtttttaac tctttgtgtt gactcaatac
    12181 gaagtggaca cctgttaaaa tcggacgact ctcttgtgta cttgcagcaa aggctgtttc
    12241 attaataatt ttcttgagta attttgtttc aagtattaaa ggagtgcttg ctgaaatttc
    12301 ttggattcgt ggatattgtt cgctatcttt tccttttagg gtaatttctg atttgccact
    12361 ggttaaaaca atttgatttt gttcaatttc tttaaaatca agagttacat caggtaaact
    12421 agatactaca ttgataaaga aagaagcttc aagaaggatc gaacctaaag aagtaattaa
    12481 caaaccagca tcttcatttt tttgagaaat aaaattttca attgaaattt gaccatttga
    12541 accaattaaa gtaatacctt cattggtcac gtcaattttt actgttgata aaataggaat
    12601 ggcattttta gaactaatag ctctcttagt agtatttaat gcttgtagaa ataaattttt
    12661 attaattgaa aaatgaatca tggattctcc tttatttatt tttagtatta gaaagataat
    12721 agtaataata gagtctgtga aatctgtgga aaacagcctt taaacaagtc atatcaagtt
    12781 ttttagcttg tttaaaaaat gtggataaaa aagataaaaa actaaaagtt atccacaaat
    12841 tatttgattt tctttttgat tgattcaatt tctaaacgta aattatcgtc ttgatcaatc
    12901 aaagatttta ttttggcatg agcatgaatg actgtggtat gatcttttcc cccaaattcc
    12961 ttcccaattt ttggaagact attatctgtt agttctctag ataaatacat ggatacttga
    13021 cgggccaaaa caatattttg aaggcgtcta cttcccttca tttctttgat actaacacca
    13081 taaaagttac caacttcagt ttggatttta tcaattggga tgacgagcat ttggctaaca
    13141 tcttgtttgc gggctctaat ggcttctgca gcaatatcaa tagtgatatc cttgattttt
    13201 tttactctgg caattaaagt gatgtcgttg atggctccct caagatctcg aacatttgaa
    13261 tcaaattgcc cagctaggta ttctagagta tcactttgga aattgtagcc taaatgttcc
    13321 gttttacttt gtaaaatggc aatacgtgtt tcaaagtcag ggggtgtgat agtttgtgtc
    13381 aatccccaac taaaacgcgt gacaagcctc tcctcgagcc cttctagatg ttttggacta
    13441 cgatcactcg ttaggacaat ctgtttttgc ttgtcatgaa gggcgttaaa ggtattgaaa
    13501 aattcttcct gagttgcgac tttttttccg ctgagtgact ggatatcatc gattaacaaa
    13561 agatcaagac tacgataggt ctttttaaac ttttccattt ccccaagtct taggtgatca
    13621 agaaagtcat taataaagct ttcggcaggg atatatttaa cacgcgcatt aggaatattt
    13681 tttagaattt catttccaat agcgtttaat aagtgagtct taccaaggcc tggtcctcca
    13741 tagataaaaa gagggttata ggtcagagcc aaatcttcag agacagctaa agcggctgat
    13801 acagcccaaa catttccatc cccttgaata aagttatcaa aggtatactt ttcttttaat
    13861 cccgtatctg aataaggaat agatactaac tttggactat agtcataaag agttaaattt
    13921 gtagcttctt caacttgtga gctagtcgta tcttgaggtt tggtgaaaat atagtgggga
    13981 gttatttcag cgtcataaat ttcaaaacca gctactacaa taatatcttt tagttgtttt
    14041 tcccagacca tttccatttc agagcgaggt agaaatatag tggcaacatt ttcctctacc
    14101 ttgataagtt cagcttgaat agcatagaaa tcatacatgg atcgagtcag tctttcttgt
    14161 gcaaattcta atatacgatt ccaaaattgt ttttctttca atcctttctc ctcctttact
    14221 atttaaaagt ttaatactag agatatttta ccatagatag caagaatttt ccacaagctg
    14281 tgaaaaataa tccacaatgt taccaaactt tatccacagg ttggggataa aagaagaaat
    14341 tattgattta ttaagctttt tatcgaaaaa catagtggat aaccttgtga gataaaaaaa
    14401 taaaagaaca gccttatttc aggctgttga taattctact atattcttct tgatttgaaa
    14461 aagaaataat gatttttcca ctgtcttttt tagatagttt aatttctaca tctaatccga
    14521 gtagttttct taactgtttt tcttcatttt gtatgaaatg attagttttt tgctgtttct
    14581 tttgtttttt ctctgtcaga agagcttcta atttccttac agaaatatct tcttctataa
    14641 tccgttgaaa gaaatagtct tgttgttcct tatttaaccc aactagggaa cgcgcatggg
    14701 cttgtgatag tttgccattt tctacttctg aaagaatctg ttctggcaag gaaagtaaac
    14761 gaatggagtt gctgatatat ggacgagact tgcccatctt atctgcaatt tcagcatggg
    14821 tgaatccttt ctctacgaga gattcatagg cgcgtgcttc ttctattggg tttaaatttt
    14881 ctctctgtaa attttcaata atggactgga ccatcatctc ttggtctgaa atctgtttaa
    14941 caacagctgg gatagaccgt agaccagcta aaagtgaagc ccgatagcgt ctctctcctg
    15001 caaggatttc ataaccaata acaggagatt gacgaacaat aatcggttga atgaccccat
    15061 tttctttgat agactgtgct agttcatcta gtttttctct atcaaattct tttcggggtt
    15121 gatagggatt tttttgtata tctgtgatag aaatcatttc aaatttttcc atgattctac
    15181 actaacacat cttttctctt atgtaaagct ttctttacat agatgtcaat taagattcta
    15241 aatcacctga actcttgtta agtttgatag aggtagtttc ttctttcccg ttacgatagt
    15301 aggttatctt aatggtgtct ccgatagaat ggttgtaaag agcactttgt aagtctgttg
    15361 atgaagcaat ctctttgtca tctacttttg taattacatc gtatttttca aggtgaccat
    15421 tggcaggcat attactttgt accgaacgaa caattacacc agatgtaaca ttacttggaa
    15481 tattgagtct tctgatgtcg cttgtactca cattagataa attaaccatc tggattccca
    15541 aagctggacg cgtcactttt ccgttttttt ctaactgttc aataatattg atagcatcat
    15601 ttgcaggaat tgcgaaacca agaccttcta cagatgttcc tccatttgta gcaattttac
    15661 ttgaggtaat tccgataacc tgcccttgaa tattgatcag tgggccgcca gagttacctg
    15721 ggttaatagc agtatcagtt tggatggctt ttgtagaaat agcttgtcca tcttccgatt
    15781 ttaaggatac atttctattg agactggata cgataccttg agtgacagta tttgcatatt
    15841 cagaacctaa cgggctaccg atggcaatag cagtttctcc tacagttaac ttactagaat
    15901 caccaaactc agctactgtt gtcacttttt ctgaagagat tttgacgaca gcaatatcag
    15961 agaaagtgtc agctccgaca atttctccag gtactttagt cccatctgac aatcgaatat
    16021 ctactttgct ggcgccattt ataacgtgat tgttggtgac gatgtaagct tctttatcat
    16081 tctttttata aataactcca gatccttcac tagagattcg ctgagaatct gtgtcagtat
    16141 catcattgcc aaatacgcta ttttgtctgt ttgccgaata agtaataaca gaaacaacag
    16201 catcttttac tttgttaaca gcctgtgttg ttgaattttc gttcttatag gcagtttgtg
    16261 taatagtact attgttgtta gagttgttta cactactttt ttgagttagt tgagttattg
    16321 aaaaactacc caaggctcca ctaaaaaagc taatgacgat aacgactaat aattgaaacc
    16381 attttttgta aaatgttttt agatgtttca tatttgcctc catatgtttg aattactgaa
    16441 agtataaact gactagctta attataactt aaacacaaaa gttttacaca aactgtggat
    16501 aactcttttg aaattgtgat tttcttaatt gaaatctatt ttttattttg tgaataagat
    16561 gtgaaaaaat agagaatatg ttagaataga gtcatgaaaa ttaaagttgt aacagttggg
    16621 aaactgaaag aaaagtattt aaaagatggt atcgcagagt attcaaaacg aatttctaga
    16681 tttgctaagt ttgaaatgat tgagttatca gatgaaaaaa caccagataa ggccagtgaa
    16741 tcagaaaatc aaaagatttt agaaatagaa ggtcagagaa ttttatcaaa aattgctgac
    16801 cgtgatttcg ttattgtgtt agccattgaa gggaaaactt tcttctcaga agaatttagt
    16861 aagcagttag aagaaacttc tataaaagga ttttctactc ttacttttat tattggggga
    16921 agtttaggat tatcatcatc tgtaaaaaat agagccaatc tttctgtcag ttttggtcgc
    16981 ctaaccttac cccatcagtt aatgagacta gttcttgttg aacaaatcta tcgcgctttt
    17041 acgattcagc agggattccc ctaccataaa tagaaaattg acttttaatt gaatttttgg
    17101 tagaataatt gtattaggtc tcatagctca gctggataga gcattcgcct tctaagcgaa
    17161 cggtcgcagg ttcgaatcct gctgggatca atataatagc aaagctggga attttcccgg
    17221 cttttttctt aaaaaagtac actttgggag aaaaaaatga cagttgagag aattttatct
    17281 aaaacgaaat tccattttgt ataatggttt ttgtaagtta gcttacaaga aaaaacattt
    17341 taggagattt tattatgaaa aacacagtta aattggaaca gtttgtagct ttgaaggaaa
    17401 aagacttaca aaagattaaa ggtggggaga tgaggatttc aagaatcatc cttgattttc
    17461 ttttcctacg aaaaaagtaa tgaaataagg agaaagagta atggatttac ttggatttgg
    17521 gacagttatt gttcattttt taattattag tcacagttac cgtttaattt gtaaaggtcg
    17581 aataaataga aaagaattat acgtttttgg tgcttataca ttactaactg aaatagtact
    17641 tgaattttcc ttttatcttc tatatttaga taaaataggg attgaaagat ttttatttcc
    17701 tttgggctta tattcctatt ttcgatggat gaaacagtat gagagggata gaggactatt
    17761 cctaagttta ctactatctc ttttatatga gagcactcat aactttctgt ccgtaatttt
    17821 ctcctctata acaggagata attttgtttt acaatatcat ttcccattct ttttcgttgt
    17881 gacggtgtta acctattttg ttacattaaa aatcatttac tatttccatt tggaactagc
    17941 ctattttgac aaggactacc tttatccttt cttgaaaaaa gtattttttg ctttactatt
    18001 gctacatatt gtatctttcg tttcagatat ggtaagtacg attaaacatt tgaatagttt
    18061 tggaagtatt ttgtcatcta ttgtctttat ctctctcctt ttgaccttct ttgcaatgaa
    18121 ttctcataaa gttcaaatgg agaaagagat tgctttgaag cagaagaaat ttgaacagaa
    18181 acatttacag aattacacag atgaaattgt tggtctgtat aatgaaatcc gtggttttcg
    18241 acatgattat gctggaatgc ttgtcagcat gcagatggca attgacagtg gtaatttaca
    18301 gaatttacaa tgaagtttta gtcaaagcaa atcataaatt gcgttcagat aagtacactt
    18361 actttgattt gaacaacata gaagactcag ctttacgaag tttggttgct cagtcaattg
    18421 tctatgctcg aaataatggt gtagagttta cactggaagt aaaagatacg attaccaagc
    18481 ttccaattga actattggat ttggttcgta tcatgagcgt tttattgaat aatgctgtcg
    18541 aaggatcggc tgatagctat aaaaagcaga tggaagtagc agttattaag atggaaactg
    18601 aaacagttat tgtgattcag aattcatgta aaatgacgat gactccttca ggagatctat
    18661 ttgccttagg attctccact aagggaagaa atcgcggagt cggattaaat aatgtgaaag
    18721 aactactaga taagtacaac aatattattt tagaaacaga gatggaaggc agtacattta
    18781 gacaaatcat tagatttaag agggaatttg aatgaaagtt ttaattttag aagatgttat
    18841 tgaacatcaa gtgagactag agagaatatt ggatgaaatt tcgaaagaat cgaatattcc
    18901 aatatcatac aagacaacgg gaaaagtccg tgaatttgaa gaatacattg aaaatgatga
    18961 agtaaatcag ctttatttcc tagatatcga tattcatgga attgagaaaa agggatttga
    19021 agtggctcag ctcattcgtc attacaatcc ttacgctatt atcgtcttta tcactagtcg
    19081 atcagagttt gcgactctaa cctataaata ccaggtatca gccctagatt ttgttgataa
    19141 ggatatcaat gatgagatgt ttaagaagag aattgagcaa aatatcttct acacgaagag
    19201 tatgttactt gaaaatgaag atgttgtaga ttatttcgac tacaattaca agggaaatga
    19261 tttaaaaatt ccttaccatg atattttgta tattgaaaca acaggggtat ctcataaatt
    19321 gcgcattatt ggtaagaatt ttgcaaaaga gttttatggt accatgacag atattcagga
    19381 aaaggacaaa catactcagc gattttattc tcctcacaag tcatttttgg taaatatagg
    19441 taatatcaga gaaattgatc gaaaaaactt agaaattgtt ttctatgaag accatcgttg
    19501 tcctatttca agattaaaaa ttagaaaatt aaaagatatt ttagagaaaa aatctcaaaa
    19561 gtgattgaca attagcaaga aattgatata atggttatat ctgctacaga tagaaggtcc
    19621 gttggtcaag gggttaagac accgcctttt cacggcggta acacgggttc gaatcccgta
    19681 cggactattt tatccgccat agctcagttg gtagtagcgc atgactgtta atcatgatgt
    19741 cgtaggttcg agtcctactg gcggagtata gataaaaggg aacacagctg tgttcctctt
    19801 tttgtatcaa tttgtatcac caagcatttt cataaggaag tctgttattt cttgagaact
    19861 ttcttttttt ccatgtgcaa tccaagtttg gcagacacca aaaagtgcat gagttagata
    19921 gatgctacta tattctaatt cagtggtatt tagattcagt tgcataaatc gcttttgtaa
    19981 atctgtacta agcatgatat gaagtttatt tcgtaagaaa ttttggattt ctttagtccc
    20041 attttcagaa agaagggcag ccagaagtgg ttctgactct agatattcaa aaacttctaa
    20101 aatagcgtct cttttgtgat gagcatgttt ttgaaaaata tattcaaatg tatggaatag
    20161 cttgctttga tagtgctcaa tcatatcata cttatcctta tagtgagtat agaagctgga
    20221 acgactaatt ccggcttttt ctactaattt gacagtagaa attttatcaa atggctgttc
    20281 catcagtaat tgtaccatag cattttcaat agttcgcttt gtttttaagc gtttgttact
    20341 ttcttgcata tttcctcctt gtaaacaaat tagactatat gtctaaaaat agatttttta
    20401 tcttgtaatt tagatttttt aatgtataat ctattatatc aaaattttag acaatatgtt
    20461 taaaaaagga gaaactaagt ttaaagaatg gaaagcaatt taaaaaaaac caacctttat
    20521 tattgtcatg atcgggattt ctcttattcc agatctgtac aatatcatat ttttgtcatc
    20581 aatgtgggat ccatatgggc aattgtctga cttacctgtg gcagttgtaa ataatgataa
    20641 agaggcttcc tataatggta atactatggc aataggaaaa gacatggtgt ccaatttaaa
    20701 agaaaataaa accttggatt ttcattttgt agatgaagag gaaggaaaga agggattgga
    20761 agatggcgat tactatatgg tagtgacttt accaagtgat ttatctgaaa aaacaactac
    20821 attatccaat attcaatcga cagcagctta tcaatcattg acaagtgagc aacaaactga
    20881 gataagtgat tctgtatctc aaaattcaac tgatagtatt caatcggctc agtcaattgt
    20941 agctttagta caagatttac agggaagttt agaaaactta caaaatcaat cttctaatct
    21001 ttcgacttta aaaaatcaat ctaatcaagt atcacctatt acttctactt ctttgatagg
    21061 attgtcaagt ggattaacag agatacaagg agatgttact agcaaattag ttcctgccag
    21121 tcagtcgatt gcatcaggtg taaacgcata tactacaggt gttgataaag tttctcaggg
    21181 cgcaagtcaa ctaagtgaaa aaaatgccac cttgacaggt agtttggata aactagtttc
    21241 aggctcaaac accttgacac aaaaatcttc tagattgaca gcaggagttg gttaattaca
    21301 atcaggatct gggcaattag cagacaaatc cagtcagtta ctttcaggtg cttctccatt
    21361 agagaataga gctaataaat tggcagatgg atctgggaaa ctagcagaag gtggaacaaa
    21421 gttaacttct ggattggaag atttacagac aggacttgct tctttaggac aaggactagg
    21481 taatgctagt gatcaactca aatcagtatc aacagaatct aaaaatgcag agattttgtc
    21541 aaatccactc aatctttcaa aaacagacaa tgatcaagtt cctgtaaatg gaatcgcaat
    21601 agctccttat atgatatcag ttgctctttt tgttgcagca atatcaacaa atatgatatt
    21661 tgcgaaattg ccttcaggac gtcatccaga gagccgttgg gcttggttga aatctcgagc
    21721 tgaaataaat ggtattatag ctgttttggc aggaattttg gtatatggag gagttcagct
    21781 tattggttta actgctaatc atgagatgag aatatttatt ctcatcatcc taacaagttt
    21841 agtattcatg tctatggtga ccactttaac aacgtggaat agccgtatag gagctttttt
    21901 cttacttatt ttgcttttac tacagttagc atcaagtgca ggtacttatc cacttgcttt
    21961 gacaaatgat ttctttagat ctattaatcc ctggttacca atgagctatt cagtttcggg
    22021 attacgacaa acaatctcta tcaacaagtc attttcctag ctgtcatact agctctattt
    22081 actggtttag gtatgctagc ctatcaacat aagaaaatgg aagaagatta aaaaaatcga
    22141 ccgattaatg aactgcaccc caaaagttag acagaaaaaa tctaactttt gaggtgtttt
    22201 attatgaaa
//
LOCUS       DAVBCW010000021        21993 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVBCW010000021 DAVBCW010000000
VERSION     DAVBCW010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21993
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.021"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(<1..217)
                     /gene="tnpB"
                     /locus_tag="VQ514_000717"
     CDS             complement(<1..217)
                     /gene="tnpB"
                     /locus_tag="VQ514_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HET2499508.1"
                     /translation="MTIHLSSLGQVYLVCGKTDMRQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKYRFKALYWDGQGFWLLYKRF"
     gene            complement(198..383)
                     /locus_tag="VQ514_000718"
     CDS             complement(198..383)
                     /locus_tag="VQ514_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499509.1"
                     /translation="MPQPIVPVEIPQSRPFDSKKRNDILLKIRIGKLEVSFFQSLNLE
                     MVEQLLDKVLLYDNSSI"
     gene            complement(687..2249)
                     /gene="guaA"
                     /locus_tag="VQ514_000719"
     CDS             complement(687..2249)
                     /gene="guaA"
                     /locus_tag="VQ514_000719"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HET2499510.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            2391..3089
                     /locus_tag="VQ514_000720"
     CDS             2391..3089
                     /locus_tag="VQ514_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET2499511.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(3132..4028)
                     /locus_tag="VQ514_000721"
     CDS             complement(3132..4028)
                     /locus_tag="VQ514_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET2499512.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLLIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAIFFAFLIMNEQFYPIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(4037..4972)
                     /locus_tag="VQ514_000722"
     CDS             complement(4037..4972)
                     /locus_tag="VQ514_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HET2499513.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(4969..5694)
                     /locus_tag="VQ514_000723"
     CDS             complement(4969..5694)
                     /locus_tag="VQ514_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HET2499514.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDRASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLGKDRNNVELWFEEV"
     gene            complement(5778..6413)
                     /locus_tag="VQ514_000724"
     CDS             complement(5778..6413)
                     /locus_tag="VQ514_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HET2499515.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEGEEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKSLKDYLY"
     gene            complement(6415..7242)
                     /locus_tag="VQ514_000725"
     CDS             complement(6415..7242)
                     /locus_tag="VQ514_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2499516.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALAHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(7264..7600)
                     /locus_tag="VQ514_000726"
                     /pseudo
     CDS             complement(7264..7600)
                     /locus_tag="VQ514_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398793.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
     gene            complement(7601..8317)
                     /locus_tag="VQ514_000727"
     CDS             complement(7601..8317)
                     /locus_tag="VQ514_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HET2499517.1"
                     /translation="MAHGDLLYHDGLFFSAKKEDGTYDFHENFEYVTPWLKQADLAIG
                     DFEGTINKDHYLAGYLLFNAPVEVMDAIKEAGYHVLDLAHNHILDSQIEGVISMADII
                     EKAGITPIGVYTHEPRDQAPLVIKEVNAIKVALLAYSYGFNGIEQYIFQEDYNRYLSD
                     LNEDKIKAEVERAEKEADITIIMPQMGVEYRLEPTEEQKALYHKMIDWGADIIFGGHP
                     HVVEPSKTVEKDGDKKLIIY"
     gene            8840..9451
                     /locus_tag="VQ514_000728"
     CDS             8840..9451
                     /locus_tag="VQ514_000728"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET2499518.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEGIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            9496..10224
                     /gene="rlmB"
                     /locus_tag="VQ514_000729"
     CDS             9496..10224
                     /gene="rlmB"
                     /locus_tag="VQ514_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HET2499519.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEMTEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFLNRL"
     gene            complement(10263..11174)
                     /gene="trxB"
                     /locus_tag="VQ514_000730"
     CDS             complement(10263..11174)
                     /gene="trxB"
                     /locus_tag="VQ514_000730"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HET2499520.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVKEIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDSHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(11240..11464)
                     /locus_tag="VQ514_000731"
     CDS             complement(11240..11464)
                     /locus_tag="VQ514_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HET2499521.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTARARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(11542..12285)
                     /locus_tag="VQ514_000732"
     CDS             complement(11542..12285)
                     /locus_tag="VQ514_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2499522.1"
                     /translation="MIKISNLSKSFSGQTVLDHLNLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILLLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(12285..13085)
                     /locus_tag="VQ514_000733"
     CDS             complement(12285..13085)
                     /locus_tag="VQ514_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2499523.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(13243..13599)
                     /locus_tag="VQ514_000734"
     CDS             complement(13243..13599)
                     /locus_tag="VQ514_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HET2499524.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(13612..14124)
                     /locus_tag="VQ514_000735"
     CDS             complement(13612..14124)
                     /locus_tag="VQ514_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET2499525.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDINK"
     gene            complement(14124..14618)
                     /locus_tag="VQ514_000736"
     CDS             complement(14124..14618)
                     /locus_tag="VQ514_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2499526.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            14753..15199
                     /locus_tag="VQ514_000737"
     CDS             14753..15199
                     /locus_tag="VQ514_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HET2499527.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            15196..15843
                     /locus_tag="VQ514_000738"
     CDS             15196..15843
                     /locus_tag="VQ514_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HET2499528.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(15864..16445)
                     /gene="pdxT"
                     /locus_tag="VQ514_000739"
     CDS             complement(15864..16445)
                     /gene="pdxT"
                     /locus_tag="VQ514_000739"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HET2499529.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(16446..17321)
                     /gene="pdxS"
                     /locus_tag="VQ514_000740"
     CDS             complement(16446..17321)
                     /gene="pdxS"
                     /locus_tag="VQ514_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HET2499530.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(17473..18852)
                     /locus_tag="VQ514_000741"
     CDS             complement(17473..18852)
                     /locus_tag="VQ514_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET2499531.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKDKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            19147..20070
                     /locus_tag="VQ514_000742"
     CDS             19147..20070
                     /locus_tag="VQ514_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HET2499532.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGISPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            20130..20735
                     /locus_tag="VQ514_000743"
     CDS             20130..20735
                     /locus_tag="VQ514_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET2499533.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFADKAEIELVEIKTIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            20753..>21993
                     /locus_tag="VQ514_000744"
     CDS             20753..>21993
                     /locus_tag="VQ514_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET2499534.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRNFKFKFELEVLEIDEVPMF
                     NQDEKWDESFQLRFLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AITGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGAS"
BASE COUNT         6571 a         4955 c         3992 g         6475 t
ORIGIN      
        1 caaagcgttt atatagtagc caaaatcctt gaccatccca gtaaagggct ttaaagcggt
       61 atttacgtcc accacaaaag agaaaaactt gacctgagaa aggatctaat tcaaagtggg
      121 ttttaaccag ataagccagt gaatcaatgc cttgcctcat atccgttttc ccacatacga
      181 gatagacctg ccctaggcta gatagatgaa ttgtcataga gcaacacctt atccaaaagc
      241 tgttctacca tttcgagatt gagagattga aaaaaactta cttcaagctt gccaatacga
      301 attttaagca gaatatcatt tctcttttta gaatcaaaag gacgagattg tggaatctct
      361 acaggaacaa taggttgtgg cattaaaata tcctccaata gttttacttt actaatagta
      421 tacaggagga actccatgaa tgatagatac cttgttatga cgcgattact atattagaaa
      481 gttggctctc taattaaatt aattctcatt atatatatca aaagaccgca gcttgaaaac
      541 tacggtctaa ttttttgtcc aaatgtaaac cacttggaac tattattcaa tttattcttt
      601 ttagtaaatg ttcccgtttt aatcccagtt acagttttta atcctctgta accgttgatt
      661 ttacaaagct tttaattttt ttgcgattat tcccactcaa ctgttgcagg tggtttactt
      721 gtaatatcgt agacgatacg gttaacatga tccacttcat ttacgatacg tactgagatt
      781 ttttgaagta cttcccatgg aattttggca aaatcagcag tcataccatc gatagaagtg
      841 atagcacgga ttgcaatcgt gtagtcatat gtacgaccgt cacccataac accgactgaa
      901 cgaacgcctg tgttaacagt gaagtattgc caaatatcgc ggtcaagtcc agctttagcg
      961 atttcttcac gaagaatagc gtctgattca cgaacggttt caagtttctc ttcagtgatt
     1021 tcacccatga cacggatagc aagtcctggt cctgggaatg gttggcgcca tacgatatgg
     1081 tctggcatac caagctctgt accaagagca cgaacttcat ccttgtaaag agtattgagt
     1141 ggttcaatca attcaaactg catatcttct ggaagaccac ccacgttgtg gtgtgacttg
     1201 atagtttgag ctgtatccgt accagactcg ataacatctg tatataaagt accttgagca
     1261 aggaatttca catctttgag cttgcttgct tcgtcatcga atacatagac aaactcgtta
     1321 ccgatgattt tacgtttttg ttcaggatca gaaacgccag caagtttgtc aaggaaacgt
     1381 ttagcagcgt ctgctttgac gatattcaaa ccaaacttac caccgagcat gtccataact
     1441 tgatcagctt cgcctttacg aagaagaccg tggtctacga agatacagat caattgatcg
     1501 ccaatcgctt tttggagaag aaccccaacg acagatgagt caacaccacc tgatagacca
     1561 agaaggacac gtttatcacc gacggtttca cgaatttttt tgatctgcat gtcaatgaaa
     1621 ttatccattg accagtcacc tttagcctta caaatgttaa gggcaaagtt acgaaggata
     1681 tcatttccgt atacagaatg acgaacttct gggtggaatt ggataccgta aatgtgttta
     1741 tctgggtttt cgatggctgc gtatgggcag tcagctgatg tacctgtacg aacaaagtca
     1801 gcaggaatct cagtaaccgc atcaccatgg ctcatcaaaa cagtctgttc atcaggtgtt
     1861 gattcaaaaa gcgctgatgg tgtgtgagtt agggttgatt gaccgtattc acgatttcca
     1921 gcatcacctg caggaacaac ttttcctcca agtttatggg tcaataactg cataccataa
     1981 cagattccca aaattggaat tccgagttcg aagatttctg ggtcaatatc aaatgaacca
     2041 tcttcatata cagaatttgg accacctgat agaataattc ctacaggatt gacttcacga
     2101 acttcagcag ctgaaatttt atggcttttt agttctgaaa aaacaccaat ctcacggata
     2161 cggcgtgaaa tcagctggtt gtactggcta ccatagtcca atacgatgat tttttctaca
     2221 tcttgcaaat cagttgaaat gttgctcatc tttttccttt ctcaaaagaa atatcttttc
     2281 tcaatattct atcacaaata agggcgaatt accaactaaa caaggcgaag tttgactttt
     2341 tctagtttat tggggtacaa tagagaaaag atagaaatga agtgaaaaat atgctacctg
     2401 cttatatgaa aatccatgat cagataaaaa aggatattga cgagcaccgt tgggctattg
     2461 gtgagaggct tcccagtgaa agagatttag ctgagcagtt tgcggtcagt cgcatgaccc
     2521 tccgccaagc cgtatctcta ttagtcgaag aaggcgtctt agagcgccgt gtaggaagcg
     2581 gcacctttgt ttccagtact cgagtacaag aaaagatgcg agggacaacc agttttactg
     2641 aaattgtcaa atcccaaggt aaagttccct ctagccagct catttcctac aaaaaaacca
     2701 ttcccaatga gcaagaagtt gccaagctag gaatttttcc aacggacaat attatccgaa
     2761 tggagcgggt ccgctatgcc gaccaagttc ccctagttta tgaagttgct tctattcctg
     2821 aaaaattcat taaggacttt aaaaaagaag aaatcacaag tcatttcttc caaaccttgc
     2881 aaaaacatgg ctatcgtatc ggcaaatctc aacagaccat ctatgctcgc cttgctaaag
     2941 aaaagattgc ccactatttg gaagttgaaa aaggacatgc tattcttgga ttgacccagg
     3001 tttcctacct agaagatggt actgcttttg aatacgtaaa aagtcaatat gtaggcgaac
     3061 gctttgaatt ttatcttgaa aataattaga ttaccacgca aaaactctct gtcacggaca
     3121 aagagtcttt tttatttttc taagaataaa tctttcaaag aaatcaaagt tacagcaaac
     3181 aatatcattg ccataccaag aaaatcaatg ggataaaatt gttcattcat tattaagaag
     3241 gcaaagaaaa tcgccgatat tggctcaatt gaagccaaca agcttgactt gaccggtcct
     3301 atcagactgg ctcctttaag gaaagctgta taggcaaaga cagtcccgat aaggataatg
     3361 cctgcaaacg caaggagaaa atcaagacta gtcgggatat cggcctgtag aacccctgta
     3421 aaaggaaggg cgaccaaacc tgctatgacc attcccacac caatgagcaa gctgctcccc
     3481 cactttttaa tcaaggctat gggtaaaatg atatacagag cataagtcaa ggcagaaaag
     3541 agaccccaga acagaccagc aggtgtcatg gataactggt ccaactgccc atgtgttgcg
     3601 atcaggaagg ttcctccgat ggcgaatatg atggaaacta tctctcccag tgtcggtgcc
     3661 accctatcct tgatacagct ataaattaaa attccgacag gacaaacata ctgaagcacc
     3721 gtcgctgttc ccgcattggt ctcctgaata gcagacagat aggcgaattg gttgagaaaa
     3781 agaccaatca gagcaaaaat aagaagagac agcaaactct ttctatcctt taaaaagacc
     3841 agtattttat cctttgcagt agcataagcc aagagcatga gaattccacc agcgattaaa
     3901 agacgcaagt tggtcaagac cagagccgaa attccgtgtg ccattaggta ttggccactc
     3961 gttcctgaca acccccaagc aataccagcc acaactgtta gtaaagtccc ttttaaacta
     4021 tttgacatgt ctctccttac tcttctttgc gaatcaagtt catcacttga ttgcggtcta
     4081 atatccattg agctctctcg tccttttcat aggtacgatt cgataggaaa atagtggctt
     4141 cttgcttctg acgattccac atgataaagg tacctgtata gcccgtatgg tctagccaat
     4201 ctccttccaa attccatgct aaagaacgtt ccttgtcatc caaaggagaa aaattttgat
     4261 ttaagtctct tgcaaaatca tctgctaaat agtgttctaa aaagatttgt aaatccttta
     4321 tagtcgaaaa taaaccagca ctcccagcat gtctacccag gagacgagcc ttgggatcat
     4381 gcactatgcc tgcctctaca cctctaactg ttggaacagc aagctcaact ggcccaaact
     4441 tggtttccgt cattccccaa ggtttccaga cttgatcctt taaaatcaca tccaaatctt
     4501 gattaaaaat tctttccaaa ataaagccca acagcaaaaa atggacatcc gaataaagaa
     4561 aggctggctg acttcgtctg ttgagatgaa acatcgcttc ctttaattca ggggctgtta
     4621 aaagatcacg attaggaata aaaggatcaa ggtctgttgc atgagtcaag agctggcgaa
     4681 tagtgatgtc tggataatca ctctcaggta aaaaatctat taccagtcta tcaatatcta
     4741 attgacctat ttcccacaag aaggtacaaa ctgtgccaac cccaacaacc ttgctgacac
     4801 tagctagatc ataaactagt cctgcctcag tctgcaagcc atgctctggg tcactctggc
     4861 ctaaatagaa ctctgtccat tgattgtcct taaaatacgc aaaagaggct ccgggataaa
     4921 ttcctgcctc gatttgttct tctatttttt taataatctt ggtccacttc atacttcttc
     4981 aaaccacaat tcaacattat ttctatcttt accaaggaag aatttttcag acttaggaat
     5041 aaaatattca gtagactcaa acttctggcg aagacttgct atgtctaatt cattgaccaa
     5101 gaacttgagc atagataagt cccaggtaac cgtattgtcc acagtcaaat cctgcccctg
     5161 agctggaata aaatcaaggg atgccccaat ttcagatgat tccaagaaac tttcgatatc
     5221 agttgggaga tgtaattcca tagaaatctc aaatttactt aaagaaattg atgccaaatc
     5281 tgtttgaaat tcaggctttt ctcctacttc tactagactt gctctgtcat cttccgcatg
     5341 aatcaaaatc aaatcatctt ctggtgagaa aatttcaaaa gcgtagccat tttgaccttt
     5401 atataatcga tgaatcgaat ctgttttaga taagattcct tcaatttcta agggattttc
     5461 caccttgaca atcaatctag ctagtttttt tcttccctct accttacgag tacgcatact
     5521 gggagcttct tctaaaacca gcttttcaag acccgtttgg tcacctagtg acagaaaggc
     5581 cgattcttct aacaaggcct tcattccaag ggtttcaata taaaatgttt catttaattt
     5641 tctattatta gcttttaaag taggaataat ccgtacaatc tgatttacat tcataaattc
     5701 ctccgtcact ttttatttta aaggatttta gaatttttta caagatatta tgctcaaatt
     5761 taacattatt tagctattta gtaaagatag tctttcaagg attttgacaa agcctgataa
     5821 ccagcaatac tgaggtgcag tccatcagtt gtatattctt ttttgagttg gcctgcttgg
     5881 tctgtcaaac aatcaaatac tggcacaaat tccacctgca tataggcaga tgcaagctct
     5941 tgataggctt gattccagtt ctgaattttt tcattcgagc ggatatagac tgcctgctgg
     6001 tactcctctc cctcattgac aggcaaaatg gaaagcaatt taatctctgt caatggataa
     6061 tcgcgagcaa cggattgaat gatagcttcg agattattga gagcctcatt cacaggaaca
     6121 tcctttccga tatcatttgt cccaatcaga agaaaaattt tatctactgc tccaccatat
     6181 agatgagcat caagattctc tagtaacagt cctgtctgat agccacgaat tcctcgattg
     6241 acaatcgtct ttgaagtccc aaatagctcc tgtagaggat aatactcgac aatggaatcc
     6301 ccaataaaaa gaatgtttgg ttctacaaca gaaatgtgat ttaggtgacg atacttagtt
     6361 tgaatttttt cttgttcctt taggagccaa ttttctaata actgtactgc cacttcattc
     6421 tcctttttct aaccagtttt ccaagacttg ataaactcct ccttggctgt tggctggtgc
     6481 tagagcagtc gccacagctt tggctttctc atcagcattt tccatcgcat aggcaattcc
     6541 agccatttca agcatttcaa catcattttc actatcacca aaagccatga tttcttggga
     6601 tttcaagtcc cagcgcttga gtaattcctc caagccccat gctttatgaa tcccagcttg
     6661 gaggatatca atgcaaccat agccactgga tacagctcgg acacggccat caaagagagc
     6721 attgatttct tccaaaaccg aactcaaacg ttcctcacca acaaccatgc tcatcttgag
     6781 cacaccacca aagaggtcag atgttaattc atccacaaat tgcatccgtt ggtagaattt
     6841 ttcaatcatt tctggagtca taaaactttc aagatctgta aaaatcgtac cttccttgac
     6901 aaaatcaccc ttcatccccg ttacaacaaa ctggtcctga cacgctcgac ccttgaaatg
     6961 agccaaagcc ttgttgacaa tggcgtcatc ccatgtctga gcctgaatca attcattgtt
     7021 ttcaaaaata cgagcgccat tagcaacaac cagaaccact cgatccacca agggactcag
     7081 tagttgtctc atgcggtgaa tttcattgcc cgtcgcaatg acaaaacgaa tgcccctttg
     7141 atccaactga tctaaaatct tttccaatcg tgggagatca agctgacccc tagcatccag
     7201 caaggtccca tccatatctg tcgcaattac cttaatcatc atttactttc ctctggattc
     7261 aagctaatac cacttcaatc ccacatgttc attcatttct ttataggctg tatcaattcg
     7321 ttccttagtc gcttcatcta actggtcacg atgactgcca cccctctata aaatcttcca
     7381 aaatataagt ttggtaatga tacaagggat atccttctgg tgaaaaggtt ccctttggtg
     7441 ttcgattgac ccaagtagga tgagctttag ctgttccgat agttgttttt ccatccttct
     7501 tcttgatggt gacatccatg agaacaccac gttcagtcca cttagcattc tcttcatctc
     7561 ccatagattc aatccgttga ttggaaatga agttccccat ttaatagata atgagtttct
     7621 tatctccatc tttttcaacc gttttagatg gttcaacaac gtgaggatgc cctccaaaga
     7681 taatatccgc tccccaatcg atcatcttgt gataaagagc tttttgttct tcagttggtt
     7741 ccaatcgata ctcaacaccc atctgaggca tgataatggt gatatctgct tccttctctg
     7801 cccgttcgac ttcagccttt atcttatctt cgtttaaatc tgaaagataa cgattatagt
     7861 cttcctgaaa aatatactgc tcaattccat tgaaaccata ggaataggct aacaatgcaa
     7921 ccttgatagc attcacttcc ttaatgacca gcggagcctg atcacgtggt tcgtgcgtat
     7981 aaactccgat tggagtgatt ccagctttct caataatatc ggccattgaa ataactccct
     8041 caatttgcga atccaaaata tgattatgag ctaaatccag cacatgataa cctgcttcct
     8101 taatagcatc cataacttca acaggagcat taaagagaag ataacccgct aaataatgat
     8161 ccttattaat ggttccttca aaatcaccaa tagctaaatc tgcttgcttg agccaaggag
     8221 tcacatactc aaaattttca tgaaagtcat aggtaccgtc ttctttttta gctgaaaaga
     8281 aaagtccatc gtggtagagc aaatccccgt gggccataat tctggcagtt ttttcctctt
     8341 cctactcctg agacttttgc ttagtcccct cttgagaaat agtatctcgt ttttgactag
     8401 tcaagggatt ccttggaagc tttcaaacaa caagaccaag cccattgata aaccaactgc
     8461 tagcaagagt atcgccacaa atcccttatt gctccacttg cgataactcc taaaaagttt
     8521 accaagccct tcataaaacg aaaagctaaa ccaccctgat ttcgattttg tcttctttgt
     8581 atcttcattc tccctacttt cttatgcaag ccttttcttt ttattatatc acagataagt
     8641 atttctttca caattgaatt gaacttccca tctattttct ataaatccta aatgccataa
     8701 tgctttcaat tcttgtcatt ttgtgatatc atgtagaggt aatgaaagga aatgaactaa
     8761 tccacagtgg cttattccaa gtataccact tgggctttgg cagtagttaa ctgcactaaa
     8821 cataatataa ggagagaaaa tgtctgcaat agaacgtatt acaaaagctg ctcacttaat
     8881 tgatatgaac gatattatcc gtgaagggaa tcctactcta cgcgcgattg ctgaggaagt
     8941 cactttcccc ctatctgacc aggaaatcat cctaggcgaa aagatgatgc aattccttaa
     9001 acattcccaa gatcctgtca tggctgaaaa aatgggactc cgcggtggtg ttggactggc
     9061 tgctccccag ttagatatct caaaacgcat tatcgctgtt ttggtaccta atattgttga
     9121 agaaggcgaa actccacagg aagcctacga tttggaaggc attatgtaca atccaaaaat
     9181 cgtctctcac tctgttcaag atgctgctct tggcgaagga gaaggttgcc tgtctgttga
     9241 ccgtaacgtg cctggctatg ttgttcgcca tgcccgcgtt actgttgact actttgacaa
     9301 agatggagaa aaacaccgta tcaaactcaa aggctacaac tccattgttg ttcagcatga
     9361 aattgaccac attaacggta tcatgtttta cgatcgcatc aatgaaaaag acccatttgc
     9421 agttaaagat ggtttactga ttcttgaata aagaaaatcc cgttgcaaga cggggttttg
     9481 tgttataata gaggcatgaa aacaaatgat attgtctatg gtgtccacgc cgttaccgaa
     9541 gccctccttg caaatacagg aaacaaactc tacctccaag aagatctccg aggtaagaat
     9601 gttgagaaag tcaaggaact agctacagaa aagaaggtgt ccatttcttg gacatcaaaa
     9661 aaatctctct ctgagatgac tgaaggtgct gttcatcaag gttttgttct acgagtgtct
     9721 gaatttgcct atagcgagct agattacatc cttgcaaaaa cacgccaaga agaaaatcca
     9781 cttctattga ttctagatgg tctaaccgat ccccataatc tgggttctat cttgcgaaca
     9841 gccgatgcga ccaatgtttc aggtgtcatc attcccaagc accgtgctgt cggagtaact
     9901 cctgtcgttg ccaaaacagc cacaggtgct attgaacacg ttccaattgc ccgagtgaca
     9961 aacctcagtc aaaccttaga taaactcaag gatgaaggct tctggacctt tggaacggat
    10021 atgaacggta ctccttgcca caagtggaat acaaaaggga aaatcgccct catcatcgga
    10081 aatgaaggaa aaggcatctc tagcaacatc aaaaaacagg tcgatgaaat gattaccatt
    10141 ccgatgaatg gacatgttca aagcctcaat gccagtgttg ctgcagccat tctcatgtac
    10201 gaagtttttc taaatagact ataaaaaagt ttccagtcat ctgattggaa acttttttat
    10261 gattaactat gttctgtgat aaacttgtag gcttcttgac cagcgatagc tccatctcca
    10321 actgctgttg ttacttggcg aaggtctttc aagcgaacat ctccaactgc aaagataccg
    10381 tcgactgcag tcttcatgtg gctatctgtc acaatccagc ctgcctgatc ttggatattc
    10441 aattctttaa caaaatcgct aagagggtcc aaaccaacat agataaagac accaccgaag
    10501 gcttgctctg tcacttgacc tgttttcaca ttttcaaaaa cgacagattc tactctgttt
    10561 tcacccttga tttccttcac tacagaatcc cagataaagc tgattttttc attcgcaaag
    10621 gcgcgatctt gtaaaacctt ttgggcacga agttggtcac gacggtgaac aatggtaaca
    10681 gtcttagcaa aacgagtcaa gaagagggct tcttcaacag ctgaatctcc accaccaact
    10741 accaataaat cttggtcacg gaagaaagca ccatcacaca cagcacagta agaaacacca
    10801 cgactgttca gttcttcttc tccaggcact cccaaaggac ggtgtttaga accagttgct
    10861 acgataactg tacgtgtttc atatgtttgg tcatcagtca tcactttctt aaaatcacca
    10921 tggtcttcga cattttcaac ataaccataa atgtgctcaa caccaagatt ttcaagtggt
    10981 tcaaacatct tttcagccaa ttcaggtcca ctaatattag cgtatcctgg gtaattttcg
    11041 atatcagatg tattattcat ctgaccacct ggcagaccac cttcaatcaa agctactttt
    11101 agattgcttc gagcagcata caaggccgca gtcatccctg caggtccagc accgataata
    11161 atagtatcgt acatatagat tccttctttc ttgttgtaac tatctttatt ctaactcttt
    11221 cttgtcaatc acgcaaggat tatgccttga aaacaagaat taaactcata accgcaaagg
    11281 ataatactgg gacaaccaag actccaatca taatcatatc atagagatgg gcttggcgag
    11341 ccctggctgt cttatcaact cccgacatgg ctctcagacc aatccaaatc cctaaaaaaa
    11401 tcagaacaag gatggtggtc aagatcaaac tctcgaaata taaagaaaat agttgcagta
    11461 gcatgatttc tctcatttct atctttttta aagagtaaac tcagctagtc caactaactg
    11521 agttttcctt tatctattat atcaaatata agtccgtttg taactagcga agaattcttt
    11581 tgtccgctct tctttagggg tgtggataat ctcatccgga gttccagact cgatgatttt
    11641 ccccttatct aataagagaa ttttatccgc aacttgggct acaaaggaca tgtcatgact
    11701 gaccaaaatc atggtctgac ctgacttagc agcatctgca atagactttt ctacttcacc
    11761 gaccaattct gggtcaaggg ctgaagttgg ttcgtctaag agcaaaacat ctggtttcat
    11821 agcaagcgca cgcgctaggg caacccgttg cttctgtcca cctgataaat ggcgaggata
    11881 atggttttca cggtccgaaa gcccaacctt agccaactct tccttggcaa tcttagtcgc
    11941 ttcttggtca gataatttct tgacaacaac caagccttct ttcacattat caagtgctgt
    12001 tcgacgttca aataaattaa actgttggaa aaccatagac aacttacggc gtagggcaag
    12061 gatttcttct tgagtgattt tagaaaaatc aactgaaaaa ccatcaatct gaatagagcc
    12121 actgtcaggt gtttcaagat aattgagact gcgaagaaag gttgattttc cagctcctga
    12181 agaaccaatc aaggctacaa cttccccttt ttgaatatcc aagttcagat gatccaagac
    12241 agtctgtcct gaaaaggatt tgcttaaatt cgaaatctta atcattaacg aaggtctcct
    12301 ttcacatctg tttgcactgt atcaggtgca gaaatagcca tttttctctc gatgaaacga
    12361 ccgaggcttt caattccgat attgactacc caataaacaa gggcaacaga gatgaagcgt
    12421 tcaaaatagc gataatcagc tccacctaga atctgagctt gggcaaagac ttccacaaca
    12481 cccgcactaa aagctagaga tgttcccttg gtcaaaccga tgagggaatt aatcaaggtt
    12541 ggagtagcta ccaccgctgc attaggaata atcactcgtc gataaacttg cgctcgggtc
    12601 atacccagac tgcgcgccgc ctcaatctca ccaggattaa ctgagagaat ggctgcacga
    12661 atggtttcac tagcataagc tgcctcatta aaggcaaaag cgacaatcgc aaaagctgca
    12721 gctggaatcg cattgatatt gagaccagtt ccccattgct gattgagggc tttcaaagcc
    12781 aaagggattc cgtagtaggt caacatgagt tgcaccaaaa tcggtgtccc ttttaagaaa
    12841 ctaacaaaga aggcctgcaa gggatataaa atcttgacac gattgatctt cacaatggca
    12901 aaaagaagcg ccaaaaccaa gccaaaaagg gcaccaccaa ttgtcaacat aattgttgtt
    12961 ggaagttgtt ggacaattct agggattcca tcaaagaccg aacgtaggct aaacagcttg
    13021 ccatccggaa tcaattgcat caagttttgg taccaatctg atgctaaaat cgttgtaaca
    13081 ttcataaaaa catcctcttc tgataatgat tcattctacc atgcttctgc cgtcaatgaa
    13141 attatttgct acggtcagat acagactttg tctacctact agtttatcat actttgaaac
    13201 agggaagtta tatattttat ctatcaaaga gatagataaa aactatttca gtcccagttc
    13261 ttcataagat tttcgataac cgatttgctt aacagttcca ttttctacta aaatgggccg
    13321 ttttaacaac ataccgtcac ttgctagcaa ctcagccgct tcttggtttg acaaacttcc
    13381 taccttatct tttagcccta attcacggta tttgatacca ctggtgttga aaaattgctt
    13441 caattcaaat cctgaggttt ctagccaatt caaaatgact tcttggctag gtgtttcttc
    13501 cacgatatgg acggctttat agtccacacc taattgattt aattcttgtt ttgctttttt
    13561 acaagttgaa catttggggt attcgataaa ttctaacatg tgcttctcct tctatttgtt
    13621 tatatcttta aaatctacgc cttcatgctc caagagccaa gctttctttt ccactcctgc
    13681 agcatagcct gtcagacgct tgcctgctcc caacacacga tgacaaggta ctaggataga
    13741 ccaaggattg cgtcccactg ctccaccaat tgcttgagca gaagccactt gcaggtcttg
    13801 agcaatttgt ccataggtca ctgtctgacc ataaggaatg ccctgtaaat agtcccaaac
    13861 tctcttttca aaattcgttc cgattggcgc caagagcaag ttggataaat cctgaggctt
    13921 gcctttaaag taatcatcta agcaagcaat aactgggtct aaaataggat gactaacaac
    13981 ttcttctatc gtttcatctc ctagtcccct ctcaaaatgc ttctgctcct gaacccaaat
    14041 tccatacaaa taatggtcat cagctacaag tgatagaata ccaattggtg aggagtagag
    14101 tatttttaca tacttctttt gcattatttc tttaattttt gataggccaa gcgttcgcca
    14161 tctactggca ccttaccaac ttgtttaaaa ccaagttttt caaaaatatg ttgcataacc
    14221 ttgttttcag catgcgtatc tgagcgaaaa tcaagataat caaaaccttc aatcaagccc
    14281 tctaagaagg tttgagcaac tccttttccc tgcacatctg ctgccacagc aatacggtga
    14341 aagactagat actctgactc tccagcttgc cagtttccct cataaatagc ttcataggcc
    14401 tcctctggac tcttggtcac agcagcatag gctagcagtt ctccctcttc caaggctacg
    14461 taggcttgcc ctgagatgat atcatcgata ataacatctg catttggata gccattttgc
    14521 cactggtcac taccagcatc tgctaaacat tttttggcat cctccatcac ctgcatgatg
    14581 gcatctactt catttggaaa agctaaacga atttccatct tttttcctca tttctgacta
    14641 gtttctccca tcatagcata atttaagtat ttctacaagc agttaaaact tgactttgtg
    14701 tttttgttat tttataatct agttatcaaa actatataaa aaggagttga aaatgaaaac
    14761 taccttttcc tacccaaaat gggcagaaat tccaaacatt gacctctatc tggaccaggt
    14821 tttgctctat gtcaatcagg tctgcgcccc tatctctcct aataaagaca agggcctaac
    14881 agcatctatg gtcaacaatt atgtgaaaaa tggttacctg acaaagcctg acaaaaaaaa
    14941 ataccaacgc caacagattg cccgtttgat tgctatcaca accctcaagt ctgtattttc
    15001 tattcaagaa atagcccaga cacttaatac tctacaaact caagcaagtt cagaccaact
    15061 ctacgatgct tttgtggact acatgaacca aggaattgac ccagctaacc ctattatcca
    15121 aaccagctgc caaaccgtca aactctatca tcaaactcta gacttaatcg atcatactca
    15181 agaggaggta atccaatgaa cactagtctt aaactcagca aacaactcag ttttggagag
    15241 gagattgcta atagcgtgac ccatgctgtg ggtgcagtca tcatgcttat cttgctgcct
    15301 atttcatcca tctatagtta tgaagcacac ggatttttat cctctatcgg cgtttccatt
    15361 ttcgtcatca gtctctttct catgttccta tcatccacca tttatcactc tatggcctat
    15421 ggttcgaccc acaaatatgt tttgcgaatc attgaccatt ctatgattta cgttgccatt
    15481 gccggctcat acacgcccgt tgtcttgacc ttgatgaata actggtttgg ctatctgatt
    15541 attgtcatcc aatggggaac gaccatcttt ggtattctct ataaaatctt tgctaaaaag
    15601 gtcaatgaga aatttagcct tgctctttac ctgattatgg gctggttggt tctggctatc
    15661 attcctgcca ttatcagtca aacgacaccc gttttctgga gtctcatggt aactggcgga
    15721 ctctgttata cagttggagc tggattttat gccaagaaaa aaccttattt ccacatgatt
    15781 tggcatctct ttatcctagc tgcgtccgca cttcaataca tcgctattgt ttattacatg
    15841 taaaaaagtt gagaaattca atctcaactt ttttctttac acatattgat aaagtactgg
    15901 tgcaagcgca catcatcagt caattctgga tgaaaagaac ttaccaacat atttttttct
    15961 tgggctgcaa caatttgatt gttcactgtt gctagaattt ctacaccctc accaacacta
    16021 ctgataatcg gaccacggat aaaggtcatt ggaatcttgc caactccctt acattctgct
    16081 tccgtgtaga aacttcctaa ttggcgccca taagcattac gctcgaccac catatccata
    16141 gttcctagat gactctcttt ctgagaagtg atttccttag ccagcaaaat taagcccgca
    16201 caggtcccaa acactggtaa gccagataga atggcttctc ggatgggaag tagcatgttc
    16261 tggtcacgta agagcttgcc catggttgta gactcaccac caggcaaaat caaacccgac
    16321 aagtcactct gatcttgctg aaaatcatct agatttctga gttctacact ctcgacacct
    16381 aattgatcta gcacttttgc atgttctgca aaggcccctt gcaaggccaa tattccaatt
    16441 ttcatctatt ttcctcgttc agccatgaga atttggattt cattttcatt aataccaacc
    16501 atggcttctc ctaaatcttc agagatttga gctaggattt gaggattacg gaagttagtc
    16561 acagccttaa caatggcact cgctcgttta acaggatctc ctgacttgaa aatacctgaa
    16621 ccgacaaaga ccccctctgc ccctaattgc atcattaacg cagcatctgc tggcgttgca
    16681 acacctccag cagcgaaatt tacaactggc aattttccat gttcatgaac atattggacc
    16741 aattctacag ggacttgcaa atccttggca gcaacataaa gctcgtcctc acgtaagttt
    16801 tgaatgcggc gaatttcctg attcatcata cgcatatgac gaacagcttg gacgatatcc
    16861 cctgtccctg gttctccttt ggtacgaatc atggaagcac cttcagcgat acgacgcaag
    16921 gcttcaccca aatccttagc accacagaca aaaggaactt ggaattcttt cttgtccaca
    16981 tggaaacggt catcagctgg agatagaact tcactctcgt cgatataatc aatttcaata
    17041 gcctctaaaa tctgagcttc aacaaaatgc ccgattctga ccttagccat tactggaata
    17101 ctaaccgctt cttggatttc cttaatcatc tttgggtcgc tcatgcggga aactcctcca
    17161 gctgcacgaa tatcagccgg aattcgttcc aaggccatca cagctgccgc accagcagct
    17221 tctgcgatac gagcctgttc aggattctgc acatccataa taacaccacc cttgagcatc
    17281 tgtgccaagt ttttatttag ttcataacga ttttcagtca tttgttttat cctcatcatt
    17341 tgtattggta gctacctttt cattttaagt cagaacagaa tgaagcacaa gataaaaaaa
    17401 ctataattgt atggggacag attggctaaa aactatctga ccttaactta aggccagata
    17461 gctcattcat ttttattttt cagccgtaag ggcagccatt gtgatgtagt tgtatggttt
    17521 attgaagtgt ggcaagaaga agaggtctgt caatgccaat ttatcaattg tcacatgctc
    17581 ttggatagca agtgagaaca tgtggattcc catgctaatt gcaatatcat gtgaaaccat
    17641 ttgggcacca agaatttcac ggctatcttt gtcaaagaca atcttaattg ctacttcatg
    17701 gttgtcatgt ttcatgaatt ctggtttttg aagatcgtta aagcctgttt cagttgcgtt
    17761 gtaaccagca gctttcgctt tttcaagagt caaaccagtt gaaaccatgt gaagaccgta
    17821 gattgagata ccatttgaac cttgaacacc gattccttcc aattcatgtc cacaagcgtt
    17881 gtaggcacca acgataccag tgcgaacagc atttgaagca agagcgatat agcttgtatc
    17941 tttacgagca ttgtcataaa cagtcgcaca gtcaccaaca gcgtaaacgc ctgggataga
    18001 tgtttcttgt ttcttgtcta caaggaaggc accgttgcgg aagagttcga tcttaccatc
    18061 agcaagggct gtgtttggac ggaaaccaac tgcaaggata accatatcca cgtcaaagct
    18121 ttctttgtca gtaatcaagc gttcaacttt accatcacct tcgattgctt taacagtttg
    18181 acctagagcc aagcggatgt tgtgatcttc caagttcttc gccatcattt gtgtgaagtc
    18241 tttgtcatag taaccgttca agacagtatc aacgatatca acaaggacaa cttcttttcc
    18301 aagacgttca aaggcttcag caagttcaac accgatgtaa ccaccaccaa caacggcgat
    18361 acggtcgaga tgttggctct tgtcagaaag tttattgata acttcttcag cattttggta
    18421 caatttcacg aattgtacgt tttcaagagt tgctttaaat tcgcggtttc ctttaacaat
    18481 ttcaacacct tcgattggtg gcaagattgg tgtagaacct gtagcgaaaa tcaatttctc
    18541 gtatgattct ttgtgttctt ttccttcaac ttctgctgta accactttgt tatcatagtc
    18601 gattgaaaga actggtgaat tcatataaac cttagcacct ttagcttcta atttgtcttt
    18661 atcagaatag aacaagcctt cagcaccgtc aatttgttca ccaatccaaa gagccattcc
    18721 acatcctagg aaagagatgt tagagttttg gtcaaataca acaatttcgt tctcatttcc
    18781 aaaattatcc aacatggtat tgatacatgc tgtaccagcg tggttagcac cgactacaac
    18841 gattttactc ataaaaaatt tcctgccttc attctaaatt tactttacta gtataccatt
    18901 taccgttagc gtttacaagg gggggactat tatttcagca ttttttatca aaaataattc
    18961 cccatttatc ctgttttcaa gcctcatgaa ttttatgagc atatttcttg actttttcca
    19021 aattttgttt gtgaaaattc taacttcatg gtattataat aacatgaaaa taaaatgtaa
    19081 gggcttcaat ttagatgatt atttgatatt gcaacttatt ttctataaga gaaaggagtt
    19141 ggtagcttga ctcttcgttc acattctgaa cggctaatgg ggactactat cactatttca
    19201 ttagtggatg agcaagccga tatctttctc caaaaatcct ttgacttgct caaagagctt
    19261 gaataccgct tcaacgccaa tagtcaagaa tctgagttga tggaaatcaa ttatcaagca
    19321 ggaatatccc ccgtcacagt tcatccagac ctgtttgagc tgatttcact tgggttagag
    19381 catagcctag cgctctctag ccacctcaat atcagcattg gtcccttaat tcaaacctgg
    19441 cgtatcggtt tttcagatgc caaggtcgcc cagcctcaag aaattgaatc ggtgctgcct
    19501 ctaatcaatc ctcatggtat cgagttagat tcttctactt ctactgtgtt tttaaaacag
    19561 aaaggaatga agatcgatct aggttgttta gccaaaggat acagtgcgga taaggttgcc
    19621 caatttctta ggaaagaggg ggtgacttct gccttgatca atctgggagg gaatatcctg
    19681 accattggaa aaaatcaggc aagaggggat aacccatggc aaatcgggat tcaagaccca
    19741 gccaatccta ggggaaatca tttaatgacc atccctgttg tcaataaatc tgtcgtgact
    19801 tcaggcattt atgaacgtca cctgaccgtc gatggacaag attaccatca catttttgac
    19861 agtcaaacag gatatcctgt tgaaacggaa ctagcgagtc taacaatcat ctctgataaa
    19921 tcagtcgatg gcgaaatctg gacgactcga ctctttggag aaagacctgc ttctatcctc
    19981 tggcaagtcg aaagtttgga gggcatcgaa gtcatcctca tcgataaaga aggccaccta
    20041 agctgttctt caggaattcc tactctatag agacaactgt ttcaatggaa ttttaaaatt
    20101 gtattatgta aaaagagaaa ggaaatatca tgttaaaact tattgctatt gttggaacaa
    20161 attcaaaacg ttctacaaac cgtcaattgc ttcaatacat gcaaaaacac tttgctgaca
    20221 aagctgaaat tgaacttgtt gaaatcaaga ccattcctgt cttcaacaaa ccagctgaca
    20281 agcaagtacc tgctgaaata ttggaaattg ctgctaaaat cgaagaggca gatggcgtta
    20341 ttatcggtac tcctgagtat gatcactcta ttccagctgt tttgatgagc gctcttgctt
    20401 ggttgtctta tggtatttac ccacttttga acaaaccaat catgattaca ggtgcttctt
    20461 acggtacgct tggttcatct cgtgcccaat tgcaacttcg tcaaatcttg aatgctcctg
    20521 aaatcaaggc aaatgttctt ccagatgaat tcttgctctc acactctctt caagcattta
    20581 acccaagtgg cgacttggtt gaccttgatg ttatcaagaa attggatgcc atctttgatg
    20641 acttccgtat cttcgtgaag attactgaga aattgcgcaa tgcgcaagaa ttgcttcgca
    20701 aagatgctga agactttgac tgggaaaatt tgtaagatag gagaccgaaa gaatgaaatt
    20761 tgttggactt gttggatcaa actacgatca atcatataac cgcaaactct tggaatttat
    20821 ccgtcgcaat ttcaaattca aatttgaatt agaagtactt gaaatcgacg aagttccaat
    20881 gtttaaccaa gacgaaaaat gggacgaaag cttccaattg cgtttcttgt ataacaagat
    20941 tacacgtgct gatggtgtca ttatcgccac tcctgagcac aaccacacta tctcagcttc
    21001 actcaaatct gtacttgaat ggctttcata cgaagttcat ccatttgaaa acaagcctgt
    21061 tatgattgtg ggtgcatcat actatgacca aggaacatca cgtgctcaag ttcaccttcg
    21121 taaaatcctt gatgctccag gtgttaatgc ctacacgctt ccaggtaacg aattccttct
    21181 tggtaaagct aaggaagctt ttgataacaa cggaaatatc accaacgaag gcactgttaa
    21241 attccttgaa acttgcttag ataactttgt taaatacgta ggagtcgttt cgaaattgaa
    21301 aaaaccaaaa ccaatcgaat cagaagactt ggattgtgga aaaccaatcg ctacaaccat
    21361 cacagaagtt gaccctgacg atccagaatg ggtagaaaaa gttgcagcaa tcactggtgc
    21421 tgtttctggt gatacctatg tcaaattgga ccacggtatc ctaacagtta accaaattga
    21481 tatgttcttg aaagctatgc catttgaatt gacatacgct gacgacaaca accaattcct
    21541 ctactacaac aacgctcacc aagatccaga cactatgttc gctaaacgtg taccacctca
    21601 atcaggtagc cgtatgtcga ctgttcatgg ttctcttcca ccagcacgta tgaagaacgt
    21661 agagtgggtt atcggaacac ttcgcaacgg aaaccaagaa tacgtccgta cgatcgttcc
    21721 aggttctcct gcaggtgtta tcaacactca caactaccaa gcaatgtact atcctgatgg
    21781 atcatacgct ggtatcaatg aaatcgtctt taacttccaa ccatggcttg actggtacct
    21841 aaaagagact ggtcaacgtt tggtgggtgg tagcggacca tttgctcctg ctgccggagg
    21901 tcatggagac gccgatgcta cttctggcgc ttctgattca ggggatgctg gaggccacgg
    21961 tggtgacgca gacgctacag ccggtgcaag tta
//
LOCUS       DAVBCW010000022        21218 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVBCW010000022 DAVBCW010000000
VERSION     DAVBCW010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21218
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.022"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(295..2022)
                     /gene="ezrA"
                     /locus_tag="VQ514_000745"
     CDS             complement(295..2022)
                     /gene="ezrA"
                     /locus_tag="VQ514_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HET2499535.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLEAGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2104..4050)
                     /gene="gyrB"
                     /locus_tag="VQ514_000746"
     CDS             complement(2104..4050)
                     /gene="gyrB"
                     /locus_tag="VQ514_000746"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HET2499536.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFIPDPKIFTETTTFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4065..4628)
                     /locus_tag="VQ514_000747"
     CDS             complement(4065..4628)
                     /locus_tag="VQ514_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET2499537.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEDILNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4713..5267)
                     /locus_tag="VQ514_000748"
     CDS             complement(4713..5267)
                     /locus_tag="VQ514_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HET2499538.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            complement(5278..6501)
                     /locus_tag="VQ514_000749"
     CDS             complement(5278..6501)
                     /locus_tag="VQ514_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET2499539.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVSLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6538..8988)
                     /locus_tag="VQ514_000750"
     CDS             complement(6538..8988)
                     /locus_tag="VQ514_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HET2499540.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLEPRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQAMGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPFVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIIGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9187..10116)
                     /locus_tag="VQ514_000751"
     CDS             complement(9187..10116)
                     /locus_tag="VQ514_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HET2499541.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELLVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARSLIQEGNFSH
                     DQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVK"
     gene            10256..10543
                     /locus_tag="VQ514_000752"
     CDS             10256..10543
                     /locus_tag="VQ514_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HET2499542.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10577..11911)
                     /gene="ciaH"
                     /locus_tag="VQ514_000753"
     CDS             complement(10577..11911)
                     /gene="ciaH"
                     /locus_tag="VQ514_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HET2499543.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(11901..12575)
                     /gene="ciaR"
                     /locus_tag="VQ514_000754"
     CDS             complement(11901..12575)
                     /gene="ciaR"
                     /locus_tag="VQ514_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HET2499544.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(12684..15230)
                     /locus_tag="VQ514_000755"
     CDS             complement(12684..15230)
                     /locus_tag="VQ514_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HET2499545.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHIAYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(15354..15794)
                     /locus_tag="VQ514_000756"
     CDS             complement(15354..15794)
                     /locus_tag="VQ514_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HET2499546.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDVILDSQNQSVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTPESKVVLEEFRKVYPL"
     gene            complement(15769..16638)
                     /locus_tag="VQ514_000757"
     CDS             complement(15769..16638)
                     /locus_tag="VQ514_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HET2499547.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLETICSLYSDEEVWYRFTELTNTEANCLVGTKEFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSVIFPFVNDADQLKQAITVLRQYGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDLESPIMLDMLRDMQDKA
                     RKNKINFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(16680..17408)
                     /locus_tag="VQ514_000758"
     CDS             complement(16680..17408)
                     /locus_tag="VQ514_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2499548.1"
                     /translation="MELFKTWKKNMVLYGLKSQIGTVYRNNDRTTSFYDVGNFLYLAG
                     ELDSRFWEDFVRKYGLDYKIIISENTNWQDFLHRKVGLNSFTRYSFKDKANFQVEFLN
                     NLVTHLEEGYNIVPIDNHIYNCFSTEEWSQDLQGDFESYQDFVLKGGFGFVILKNNEL
                     IAGISSGLVYRKAVEVEVATRPNEQGNGFAKKLGAAMILESLNRDMFPLWDAHNEASK
                     KVAEFLGYELSEPYEAFELEEILI"
     gene            complement(17416..17895)
                     /locus_tag="VQ514_000759"
     CDS             complement(17416..17895)
                     /locus_tag="VQ514_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2499549.1"
                     /translation="MEIKKHFGVYAVCLENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELTNFMVDHVMALYDVEMNESAPQV
                     TTSEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGFPELDKTSYMNWKVK"
     gene            complement(17906..18604)
                     /locus_tag="VQ514_000760"
     CDS             complement(17906..18604)
                     /locus_tag="VQ514_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HET2499550.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(18597..18863)
                     /locus_tag="VQ514_000761"
     CDS             complement(18597..18863)
                     /locus_tag="VQ514_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HET2499551.1"
                     /translation="MKNVELKEKNMTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTI
                     EQNGLALEMTPYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            complement(18929..19855)
                     /locus_tag="VQ514_000762"
     CDS             complement(18929..19855)
                     /locus_tag="VQ514_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HET2499552.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFNLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCQLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(19930..20745)
                     /locus_tag="VQ514_000763"
     CDS             complement(19930..20745)
                     /locus_tag="VQ514_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499553.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            complement(20749..21069)
                     /locus_tag="VQ514_000764"
     CDS             complement(20749..21069)
                     /locus_tag="VQ514_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET2499554.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLTKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
BASE COUNT         6416 a         4659 c         3714 g         6429 t
ORIGIN      
        1 aagttacttc aaagtacaga tttgaagctg cagataagac tgacgaaatc actttaatac
       61 taaatgaaaa tcaaaaagca aactaagaag ctagcctcag gctgtacttg agtacggtaa
      121 ggcgacgctg acgtgatttg aagagatttt cgaagagtat aaaacactgt tttgaagtgg
      181 tggataaatc ggctgggtaa aaactcaatc tcaggagaaa atgaagtaaa tctccccaca
      241 actatagaaa aagatttgat tctgctcctc acacaataaa atctttttct tttattaaaa
      301 acgaatcgtt tcacgtgttt tctcatatga ggtaacaaag cgattggtta caccaggctc
      361 tgccacttcc aatgcttgag aaatcttgtc aaatgaagcg tgataatcaa attctttttc
      421 aaaaatatct aaagcttcgt taaatgcttc ttgaatgcgt tcatcaaatg agcgatagcg
      481 gttagaatat tgcaagagtt gctctgtcaa agttgcatat tgtacaatat tataagtttc
      541 cgtttctaaa gcttccatat cattcgttgc aatttcaaga actcgggtaa cagattcaat
      601 gttaatcatt ttttgttcta actcaaccat taaatcctcg gtattattgc ttgccgtaaa
      661 gaataacttc aagaaagttt gtggaatacc tggcagattg cgtttttcca tgtatcgctt
      721 gatagtatgg agacgattga cataaacatt ggccttttga cgtgcattaa tatcatcttt
      781 ctcaatttgt gtcaggcgct cactaactga aatttgctca tcttcaatat cttttagttg
      841 agtttgtaaa tcctcaagat tttcttcaag aactgaataa gcttgggttg gttcttcttg
      901 atttgaagtt acctcaacaa tagctgcctc aaaactctct aattctgtct gaatacgacg
      961 aacatggctt gcagctgtct caggaagtaa ataggtcttg ttcaaacgtg caatatcttc
     1021 tcccaataaa gtattattct ctttcatatg ttgaaggtaa gttggaagag ttgcaagtag
     1081 attttccact actttctgag cagcaatttc tcgagtaaaa atatcataca aggcattgat
     1141 ttcctcttgt gcctgtccat tctcatattc ggcattatcc aattccaact gacgaatatt
     1201 ctcttggttt ttcttgaatg cttcataaag caagtggaaa cgcgcttcaa tatccgtttc
     1261 aacaaaatga taattagcat caattagttt acgataaccg gcttccaaat cctgtaattg
     1321 atctggcaat tctgtagaaa gcgtcgtaac caaggctgga acacgatcca caatatgact
     1381 taaggccaaa atgtgatttt ctgtattatc caaaatcact gcggcttcca cagggtcacc
     1441 cgatgaattc aaggttacaa attgtgaaaa ttcagattgg atattttcta attgtttttc
     1501 aatttcatcc aaggcttgac catactgttc tgaattttca gcaactctat gctgaagttc
     1561 ctcaaataaa tccaaagcat gaagaacacg accactattt ttagattctt gcttctctaa
     1621 gtctgccaaa gcattgcgaa ttgccgcaat atcttcttca atcaaagtaa tttgactctc
     1681 aatttggtca atttgatgac tggccttgag aaaacgaaat gaatggttat aaccttctgc
     1741 ttcaaagaga ttattttcaa tatcggcaaa agagttgaga gataaatcga cccatttttg
     1801 attccattca cggaaagcca cttgactttg tccaatcaag tgcatatttt ttacagcttc
     1861 tacttcatca tttactggaa gattgtatag ttcttctttt ctttcttcta gcgcctctaa
     1921 tctcccctcg tttcgcttac gtagaaagat tgccactaca taagccagaa ctaaaatgac
     1981 tgcaattgca accattaaat aaattagttg tccattagac atatcaaact ccttttttac
     2041 ttgaaacaat cgtaacgatt atatcatatt ttttagacca agggaactac ttctccctat
     2101 tttttagaca tcaagtgtac tatagacagc attttcttcg ataaactcac gacgaggctc
     2161 tactcgatcc cccatcaaca tatcaaagat tttatctgct tctgcagcat catctacaga
     2221 aactctagcc atcaagcgat gttcgggatc catggttgtt tcccacagct gatgatcgtc
     2281 catttcacct agccccttat aacgctgaat agtcggtttg gtacgacctt cactataacg
     2341 ggctaaagct tcttggagtt tgatttcttg atctgcaccc ggctggatat attctttaat
     2401 ctcgcttcca accttgacac catagattgg tggttgggca atataaacat aaccagcttc
     2461 taggattggt ttcatataac gataaatcaa ggttaaaaga agggtacgaa tatgggctcc
     2521 atcgacatcg gcatcggtca tcaaaacgag tttttggtaa cgggctttcg aaacatcaaa
     2581 ttctgcgcca aatcctgttc ccatggctgt gaaaagacta cgaatttctt cgttagctag
     2641 aatcttatcc atacttgctt tttcaacgtt caaaatctta ccgcgaattg gaaggatagc
     2701 ctgaaactca cggttacgac cagatttggc tgatccacca gctgagtctc cttcgacgat
     2761 gaagagttct gtttcagcag ggttattaga agaacagtct gctagtttcc ctggaaggtt
     2821 ggaaatttcc aaaccagatt ttttacgtgt gacttcacgc gcacgcttgg cagccacacg
     2881 agccttggca gctaaaatcc ctttttccac gatacgtttg gcaatctgtg gattttccat
     2941 gaggaaatcg gagaaggctt cactgaagag gcgattggta atcttgacca cttcgctatt
     3001 tcccaatttg gtcttggttt gtccttcaaa ctgtggattt gggtgtttaa ctgagataac
     3061 tgcagttaat ccttcgcgaa catcttcccc tgttaaatta tcttcattgt ctttcagtaa
     3121 cttattctta cgagcataat cattgataac acgagtcagt gccgtacgga aaccttgttc
     3181 atgcgttcca ccttcatggg tatgaatatt attggcgaaa ctcatgacat tttcatggta
     3241 acctgttgtg tactgcatgg ctacctcaac tgtgatatca tccatctcac cgtctgtata
     3301 gattggtgta tcaaagatta catccttgtt ctcgttgata tattcaacgt aactagcaat
     3361 cccaccttca taatgataat gcttggtttg ttccaaacct tggcgcttat cagtgataga
     3421 aatttgaaga ccgcgattta gaaaggccaa ctcttgaatc cgtttattta atttatcaaa
     3481 atcaaaggtt gttgtttcag taaagatttt tgggtccggt atgaagtgaa ccgttgttcc
     3541 agttctatct gtatccccaa ccacctcaag gtctgcaaca acatgaccac gacggtattc
     3601 ttggtaatga atcttaccgt ttttatggac atgaacgtct aattgagtgg aaagggcatt
     3661 aactactgac gaccccaccc cgtgaagacc acctgaaacc ttgtatccac caccgccgaa
     3721 ctttcctcca gcatgaagga ctgtaaagac ggtctcaaca gcaggacggc ctgttttttc
     3781 ctgaatatcg actgggatac cacgcccatc atccacaaca gtaatcgaat catctggctc
     3841 aataaaaact tgaatatggc tggcaaatcc tgccaaggcc tcgtcaattg agttatcaac
     3901 aatttcccag actagatggt gaagaccttc ttttgaggtt gatccaatgt acatccctgg
     3961 acgcatacga acagcctcta agccctctaa aacttgaatt tgactggcat cataatcctg
     4021 tgcctgcaga tttttgattt cttctgtcat cttattcctt tttcttatat gtctaatact
     4081 tgtcttaaat tcacgatact ggtaaacaag tgggtatcaa gtcctgcagc ttgacctgct
     4141 tcgatatcaa tcggccgatc accaatgaca agaccagagc taatctgata cttttctctt
     4201 aaataaagca tggattcggg atttggcttt ctcttaaagc ctgagctaga agtcaccact
     4261 tctgtaaaat aagctgctat agaggttttt tctaaaattt ccaaaacctg atcatttcga
     4321 tgagagacca aaaaatgacg gccaccttga tttaaaatgt cttccaatag gtcagaaact
     4381 ccttcaaata aaatcgggtg ttcaagctct ctggcttcat tttccttgta cttttctaaa
     4441 aaattctcta aattgggagc gaatgtctca atcgcaaaag gagtagaaac ctttaaagct
     4501 tgatagacac tgtcatggtc ttgtgtgata ccatacagtg ccaatgtttc aacaaatgca
     4561 gctgttgaag tttcataatt atccagtaaa gttccaccta aatcccagat gtaatcgtga
     4621 tatttcatac ccttcattat agcatttttt tatgaaaaaa gcgatgattt ccctgagttt
     4681 tccacataaa aaacgagaga ataactcccg ttttactgat ttttaccaaa gacatctcga
     4741 tagagcattc ctgttcgtaa actctctgcg tcccctccta gttgctccac caactcgtag
     4801 gcaaaggcaa gggctgttga aggaccccga ctggttgtca actgaccatc taccactact
     4861 gtttccttga cgtagtgacc atcaaggatt tgctcttgaa cgccgtcata acaagtgtat
     4921 cgcttatttt tcaatatctc tgcttgattg agggcaattg gtgccgcaca aatggctgct
     4981 agtttcttcc cttcttgctc gaagctttgc aattcttgaa tcaaggtctg attatcacgt
     5041 aaatgtgcag aaccaggcat acctccagga agaacaatca tatcatagtc tgataaatct
     5101 ccatcaaaga catgatctgc tcttacttgg attgcatgcg aacccgttac ttgctcttca
     5161 aaaccaacca tatcacatgt gatattggct cgacgcaaga catctacaac tgtcaaggct
     5221 tcaatttctt caaagccctg agctaacata actgctactt ttaccatgat tttctcctta
     5281 tttaatttgt tttaatacaa cctttttccg tttgaatggg acttttccgc tcttttcttc
     5341 agctagatta gtctggtaac tcatcgataa aagcaaacca acaccaatca gattactgat
     5401 aatagctgat cccccttgcg aaatgaaagg caagggaatc cccgtcaaag gaagtagtcc
     5461 agtcacagca ccgatattct caaagatgtg gaagagcaac atcataatca aacctgtgga
     5521 aatataagtg tagaactggt tatttgattt aagagtaatc ttcaacatac ggtaaatcaa
     5581 catgagatag agggcaataa ccaggacaga gccaataaag ccaaaatctt ctgcaataac
     5641 cgtaaaaatc atatctgact ctcgaactgg gataagcaga ttcgaagcat taaatccctg
     5701 accaaataag ccaccactcc caatggcaat ctgcccttga gcctgctggt aagtcgttgt
     5761 ttgggcaaac tcaaagggat tgagccaagc caaaatccga ttgatttggt aggtcggcat
     5821 tccaatctgg tgaagaaaag ctcgtccgtc cttgctaata aagatagcta agaaaccagc
     5881 aactcctgtt acagcagtca caaatactgg gataataatt ttccaagaaa cccctgataa
     5941 taaaacgatt cctgagaaaa tggctacaaa aaccaaagcc gtccccaagt cactttgaag
     6001 tgctaaaaga actaggactg gaatggtaaa gagaatcatc cagaaaatta acaaaaagtc
     6061 cagcgaaacc gtgcgtctcc attccttatg tttctttgta aattggacaa tgacacgagc
     6121 caacatgagg atataggata tcttcataaa ttctgacggt tgaaataggg taattccatt
     6181 tattgatacc cagtttttgg cacccgttga tgcaactaag cttggattat aaaatacaat
     6241 cggcaagatc ataagtccca agcctaaaat atatagaaag ggggtcacct tccaaagaaa
     6301 ttctgtatta aagagcatga ccacaaaacc aatcacaagc cccaaggcaa tccaggcgac
     6361 ctgctgccct aaaatgggca gaatattatt gggataatca tgactaacgg ctatatagat
     6421 agccaccaca ccgatgacca gtagaaaaaa tactggcaag agcaaactat aatcgactct
     6481 agagtcgaga gaacgtttca tatatactaa ccttatactt tcatacaata ctatttatca
     6541 aagttcatta aaaaatctat caatagcctc gtcaacttcg gatcgagaga tggttttaac
     6601 agtcgcttct tctgctagag atgctactat ttgtttgccg tatcgttttc cgatgattct
     6661 cctatccaaa ataagagtta aggaacgttg gtattcacgt ctcatacttc ttcccaaagc
     6721 ctgttttaaa cgaataatgg ccattggcaa ttgataatca taaaaggcat ttttcccttc
     6781 ttgattcagt tcttgattaa tctttttcgt caagggttct tgaggatttt ggaaaggaag
     6841 cctcggtaca acttgaatca caaaaggatg gcttgaaaaa tcaactccct cccagaaact
     6901 tgctgcacca agcaagattt gttgttcacc tttttcaaag cgtttcttta gctgatgaac
     6961 atccccattt ttatactggg ccaagtggct aactgtaagt aaatccgata ctgctagaag
     7021 catgtcttta gcggtaaaga gaaccaaaat cggttgctgg aaagcttgaa tttccactag
     7081 taaagcagct acctctctgg catagacttc taaggaggtt tctgttacca gagggaaatc
     7141 tttgaccata accacttctt gttcctgttt tccccgagat tcaatcttga caaatttggc
     7201 ttcaggatag cctaaaaggt ctgccaaaga aaccctctga ctaatctcaa gagtagccga
     7261 tactcccatg gcttggcaac tctcaggaag taaagaggac agaagagtac ggcctgattt
     7321 tgtagaagaa atctgaattt tcttttgact cgtttcagtt acttcaagcc agtaatcacc
     7381 ttcagctgta aaatagcgaa ccagctcatc aaagccttct acttctaatt ctgaaaaata
     7441 ctgatggaga ttgtccagag aatctaagat atttttccta gatttaccag actgaaattg
     7501 ttctatcaag tagagacact caaagcgaat actttctagt atccgttgtt gaaccctgtt
     7561 ttcttctcct actaaagcct tatcaattaa atcgataata gattgtatat cgtaggtctc
     7621 ttgaagcaga ttttctagag ctaacaaaat cttttggact tcatcaataa tcagtaaacg
     7681 gtcactgaca aattcaggat tatcttcaag tctggtcacg agataggcat gattagtcac
     7741 taaaagcttg caagtctctg ccctttcttg actacgtttc caaaaatctt ccgtcacaaa
     7801 taagctctgg gatgataaat tcccatcatg acgaaggtct gctagaaaat gttggtaacg
     7861 atagagttgc ccgatttcat ccaaatctcc tgtctctgtc tcagtaagcc agaccaagac
     7921 ttgcatttta aagcgtctaa ataagcgatt ttcatcattt tcctgcaagg aatgataaaa
     7981 ggcatccaac ttcagataat tttgtggtcc ctttaagcta tgaatatctg tatggaacac
     8041 ttccttgagg cgtttacctt cttcttccat gatttgattt tgaagaatct ttgtcggaac
     8101 actaagaaca atttgtcgct ctttggattg agataaagcg ggtaagagat agccataggt
     8161 tttcccaatc cctgtcggcg cttgaatcag agagacaggt tcatctttca atagcaagcc
     8221 aacctcttta gcaaaacttt cttgttcctc cctcacttca aggttcaaca gagaaatatt
     8281 tttagaaaag tcttgagata gttttcgtgg ctccagagaa gctgccgttt tcttaaaata
     8341 cagaccttga acttggacca agtctggaga actcaggata gattggttgc gataagtttc
     8401 ctcaataacc aggtaggact catataggag agcgtcagcc atttccagca agcgttccaa
     8461 gagaccttta ggaagctgag tcatcttttc ccgtaaaaaa agaagtaatt ctgctgtagc
     8521 ttgggcatct gaaagggctg tgtgtgcgtg tttaagagga attcctaatt ctcgacacaa
     8581 aatcggcaag ctatattttt ccagttcagg gaaaaagacc tgggccaatt cgaccgtatc
     8641 aacacgaggg tttcttagct catagccttc aaaaaataaa ttttccgcca agagattagc
     8701 atcaaactga acattatggg ctacaaaaat cccatcctcc accaagtcaa atatttttct
     8761 ggcaacttgc gaaaaatcag gtgcttgcgc cagacgttgg tctgtcaatc ctgtcagttc
     8821 tttgatatga gcatccaagg gttcatgtgg attgacatcc gtcgtatagt gatcgacgat
     8881 ttctccgtcc tcaatcacga caattcccac ttggataatt ttagccttac tacctgtgct
     8941 agttgcctct aaatccacca caacatagcg attaccaatc gttttcatta taaaaaatta
     9001 taccaaaaaa agggccattt ggcacctgca aacataggat aattttcaaa ctcaagaaca
     9061 ctacttttct taaaaatccc atcgaataga gcattctagc ctgctgattt tttaaaatag
     9121 tgaagaaata cgaaaaattg agagaagaaa tgttttcatc ttccttctct caacgaatca
     9181 tgtattttat ttaaccatgt caggatcgta gtcataaaat cctgtatcaa ggaaacggtt
     9241 tttagggtga agtttacgaa cttcttcatc cagcaagaag gcttggtcat ggctaaagtt
     9301 gccctcttga atcaagctac gcgcttggat aaagagtttt gcaatctcaa caaagttctg
     9361 accaggagtg tagagccctt ctagtttcca gcgagtaaag ccatgctcca ccaattctgt
     9421 taatttgatc atcaaatcaa ggtcattgtt ggcaaagata tgggtcccat gattatcttc
     9481 aaaaatggaa tagtggctct ctggatcact tggctcagcc aagaagaggt cacgtttatg
     9541 cgtcttttca tcatcgatat gtgtaaagtt atagtagttt tgcaagagtg gacgtttaga
     9601 atgatggatg acgctagcac cgtaaaccaa aacttcagca ggaatttcca aaatctctgg
     9661 cattttgaaa agttcagctg atggaatttc acgcgccaaa acagcctcag atgcgccagc
     9721 cttttgtccc cagaagttaa tctgacgact gctagttacc atggttgaag catcgtagat
     9781 ggtcttaaat gaataaccat cgcggttaac tacgtaaaag acgcctgcat ccccaatcgt
     9841 aatatagtct gtcttgattt cttccaagaa gtctaagaaa ggcttgatac ggtccatcat
     9901 atcttggtgc atgagagcat tgaccgcaac gagcaattcc ttaccagcat catgaaccaa
     9961 cttagcgatt tcacgtaatt ggtcataact aaaggtcgtt ggcagacgaa ggccaaaatc
    10021 tttctcaccg acatagatac ggtctacgcc agcttcgagt agttgttcaa cttgttcaat
    10081 actttcagca gttgctgtaa tgacaatctt ttccataaga aaaattatac cacatttctc
    10141 caaaatcatc catgctttga aaatgaaaag ggactttatt ctttttgtaa cctttctgta
    10201 ataattctct gccgaaaaaa tgataaaata ggtatgtact gttaaggaga gaattatgcc
    10261 cgtaagaaaa ttacaatcct atgaggtaga ctatcaagaa gaattaaacc agcagcttcc
    10321 tcattaccaa gcttatacac ctgaagcgca atctgatgcc aatctcaagg aaatcctatt
    10381 ttttattaat atcgctgttt tttgtatctg tattgctatc tttagtttta tctttttagc
    10441 attaaaatta tcaactgctc ttgcctttgc cgcagcaatc ggattcagct tacttgtttt
    10501 aaaagttcaa cggtctatta tcaaacgaaa acgtagaaga taaatccaaa atatcgcccc
    10561 agttggagcg atatttttat tttttctttt tagatggtgt ctgaatggca atcttcactt
    10621 caaagattgt tcccttgggt ttattatctt tgacagtaac agttcctttt agagcatcta
    10681 caatttgctt ggctagggat aatcctaaac caaaaccacc tttttgccga gttctagcct
    10741 tgtctactcg ataaaaacgg tcaaaaattt tctttttatc ttctgtcgaa ataccgattc
    10801 cattatcaga aacaagtaaa taaagattgc gatcggtcgc cgagataaga aaatcaattt
    10861 caccatcctc ctcagtatac ttgacggcat tatcgaaaag aatggtcatc agttgtttca
    10921 gaagaagctg atctgtgaca attgttcgat ggatacgatt ttcaaaacgg aagacacgat
    10981 tattttccga agcaatcatc tcgtagtttg tgaaagttgt attaaaaaag ctagttggaa
    11041 cttctgcaag ctccggctta atcccatcat ctctccgagc taagttcagc aagcttgtcg
    11101 ttaaaaaacg catatttcgg acttcttcca aactcgatgc aatgctttcg ctcacatcca
    11161 taatggtagc ttctggctta cgaaaaaggg tctctaagcg attttgcaaa actgcgagtg
    11221 gagttcgtaa ctcatgactg gcattttcca caaaagactg ttgcttctgc atactctcaa
    11281 gcaggggcct aacactgacc ctagctagat agagactggc aagtaaagac aaaatccaga
    11341 aactagccat cacgaccaca atcaattgct catgcttttg actagcctgt tccaactgac
    11401 tggtattaat caagacagca gcatacttga tattggttga aaccgaacta atattggtct
    11461 ccatcaaaat cacacgatag atttcttcct gcccatagct attaaaaacc tgaatctggt
    11521 agatatgtcc tagttctttc ttttctaact taatcttatc caagcccaaa aatcgatttc
    11581 cagaaagaag ttgagtaaag tctttatcaa agagaatgac ttccgtattg gaactgacat
    11641 taggttttat ttctacttta ctagcgtccg ccctagcatt ttctaaatct ttaatctctt
    11701 ctgttgccct atttatagcc agctgaataa ctgcttgagg attttcactc agtccatgaa
    11761 gcttatcgtc caccgaagta taaagactcg aatgcatgac ttgtaaaata atcagagtca
    11821 ttgtagaaaa aatcagggtg aagacaccga agttgcggat aaaataacta aagtcatccg
    11881 cataccatgt ttttttaagt ttactgaaca tcttttaaaa gatacccaac actacgcaaa
    11941 gtttgcaaat tctctgcaaa agtggttccc tttaatttct tacggacttt tgaaacatag
    12001 acttcgacaa ccgaaatcgt tgtatcacta tcaaatcccc atagacggtc aaaaatctgc
    12061 gtcttaggca aaatcacatt ttgattttga aggaaataaa ctagtaaatc gaactctttc
    12121 cccagcaatt cgacaggagt atcttcaact ttaacggtat tggttgataa attaaccacg
    12181 atattcccat aagtcaaggt gttttcatta aacttccctg aacgtttgag aagggcctga
    12241 atccgcattt taagttcttc taggtagaaa ggtttggtca gataatcatc cgctcccagt
    12301 tcaaatccat gtcccttgtc atccaaactt tccttggcag tcataatcag aactggtgtc
    12361 gtaattccct tttcacgcaa ttcttttaag acttggaaac catttttttc tggcaacatc
    12421 aaatccagca aaatcaagtc atagacacca ctctcagctt cgtagagacc ttcttctcca
    12481 tcaaatacct gcataacatc cgcaaaatcg tctaaaaagt caaatactga atttgacaga
    12541 cctaggtcat cctcaaccaa taagattttt atcatgagaa actcctcctt attaaaacta
    12601 ttataccaaa tttgccttaa aaaaaactca actctctgca ttttacatga gatagctgag
    12661 ttttcttttt attttaggct tatttatgca tttccgtatt gaagaacaac tgcttcgact
    12721 gcagcttttt cacggctaat caagtcaaca cgcgctgcaa tttccttgat tcccataccg
    12781 atgttacggc taagagcaag gtcagaaagt tgcggctcaa agaactcctt gtattccgcc
    12841 aagcgttgct gagtcttaaa tacatgagca ggaaggataa caaagctatc aaagctcata
    12901 tctcctccaa gggctgcctt aatccaagcc cagttttcac gcgcccaaga ccaagctgtt
    12961 ttctgagttg cttgatgagc taggaattgg taataccaag cagacaagtc ctgtggtttg
    13021 accacaaatt tgtccttcca agaagtaatc aagttttgga tattatccgc atctgtactg
    13081 tatgcaagag ctgctgccaa ctggcgttta aagacagcat ctgttgcgtg agtataagta
    13141 tcaagataaa gtgctaacaa gtctttagtc tcatgatgtt tcatctcatt aatcagaact
    13201 tgtgaacgaa tagctgctgg gagtcctgca agattctcct tgtgtgttgc gaagatttgg
    13261 ctagcgactt gactagcttc tgcatcattt gagcgaatca tcatcgaaac agccagctga
    13321 cgaaccaatt catcctcatc tgattctccg tctttagctt caaaaccaag acggtcatag
    13381 ttatgacgag ccaatttagc aaccagtcct ttgaaggctg tttcagcatc cgttccttca
    13441 tcaataaagc gctcaagggc tgaaatcact tgagaaacag ctgaaaccac cagataagac
    13501 tcttccttag caagtttatc aagaactgga agcaagtctg cataagcaat gtgccctgcc
    13561 tcagccaaca aacgacgttc ttgaacaatt tgcagtttgc ttgtgttatc aagtgtctct
    13621 agctcagcaa gaacagcttc taacaagtct ccttgatagt cggtaatata gtgggcagta
    13681 ttttcagtgt tgagacgaag agctccttca ttttcagcaa gaagagctgc gtagccaggg
    13741 atttcgatac tttcagtttc gagtgtatca ggcaagcctt tccagttgct attgaggggc
    13801 accacccaga gacggttctt gtcttcgttc tcaccgatga agaattgttt ttgtgaaatc
    13861 ttcaagacat cattttcaac tttaacagta agaactgggt aaccaggctg ttccaaccaa
    13921 gaatccatga aggctgcgac atcacgtcct gacgcttgac caagggcatc ccaaaggtca
    13981 ctaccaatgg tgttgctgta ttggtgtttt tcaaagtagg catgcaaacc tttagcaaaa
    14041 tcagcatctc ctagccaacg gcgaagcatg tgcatgagac ggcttccttt ggcatagacg
    14101 atagcgccgt caaagagtgt attgatttca tctggatgtt taacttcgac gtggacagac
    14161 tgaacgccat cagtagcgtc acgttcaaga gcaagaggta ctccacctgt ttggaaatct
    14221 tcaaagatat tccagcttgg ttcgatggta tccacacaga cgtattccat catattagcg
    14281 aaactttcat tgagccaaag gtcatcccac catttcatag tcacgaggtt cccaaaccat
    14341 tggtgagcca attcatgggc cacaacaagg gcaacttgtt gacggctagc aaatgtagag
    14401 ttctcatcga caaccaagta aacttcacgg taggtcacaa gaccccagtt ttccatagca
    14461 ccagctgaga agtcaggaag ggcgatgtgg agagattgag gaattgggta cttaactcca
    14521 tagtaatctt cgtaaaactc gatagagcga acagcgatat ccagtgagaa atcaagattt
    14581 gaaagtggat gtgctttggt tgagtagaca cctaccaggg taccattttt agttttagcg
    14641 gtcacccctt gcaaatcacc agcaacaaag gccaacaagt aagaagacat gcgaggtgtt
    14701 gtctcaaact tccagatacc tgtttcctta cggttttcaa catcgatttc tggcatgttt
    14761 gacaaggcca attcaccttc tgcttggtca aagcgaagag agaggtcaaa agttgctttg
    14821 gcttcaggct catccacaca tgggaaagct tcgcgcgcaa aatggctctc gaactgagta
    14881 gacaagacct ccttcttgac tccatcaact gtataataag aagggtaaat ccctgtcatg
    14941 ttgtctgtaa ttttaccaga aaaggcaaga accaattcaa cttgaccagc ctcagccaat
    15001 tcgatatgaa gggcttcatt gtcatggtca actgtaaatg gacgagcttg acctgcaact
    15061 tctacagagg tgatttccaa gtctttttgg tggagggaga tgcggtcact ctgtgcttga
    15121 ccagtgatgg tcactttccc agaaaaagtc ttggtctcac gactcaaatc taaaaataaa
    15181 tcataatgtt caggaacaaa ttgcttaata aaatgttcaa ctgcttgcat agttttctcc
    15241 tattctaagt ttaagagcta ctgctcttct tcaaacaaac ttattatatc atattttgct
    15301 tgagaaaaaa ggattggaca gcgatttcca aaactttctt ccttctaaat agtctacagt
    15361 gggtagacct tgcgaaattc ttctaaaaca accttgcttt caggtgtaaa agtcagccct
    15421 acttcaccca aacagtctgt gaaaaagtcc tcatgaacct ggcgccaata ggcaagtgat
    15481 ttgtctccct caccttcctt aaaggcatgt tgcgcagaaa cttgattgaa gagttcaaca
    15541 gaaacctttg taatttcgac aatgcagaca gactgatttt gactatctaa aatgacatcg
    15601 aaggtcccta cttggggaag tggttcgtct tctagtacat agaggtcata ggctgatgct
    15661 gttgctgtct tttctccttt aaacaccaaa tccgctaaaa ggtctggttc aactccaaaa
    15721 gcccaagcat cgatttcatc tccgatcaaa ggattgattt gcttgtattt attccacatt
    15781 tcttgcggta tcatgggtac tcctttgtaa ttttttactt tcttctttta tgtgtttaag
    15841 atgatctgga tggtcaatct ctaaatcaaa aatctctgga atagaactgt agtggataat
    15901 gcacttgata cccaactgat tcattttttg tatgaaagaa gtattcagat agcctgctac
    15961 agcaaaatta atcttgttct ttcttgcttt atcctgcata tctcttagca tatctaacat
    16021 tattggactt tccagatcat gccattgact gtttctcata gtcgcaaaaa caaaggaagt
    16081 caaatcattc attccaacta caatctttga aatgcccgtt tccagtatac tagataagtc
    16141 aaaatacgct gacggtaatt caatcatcgt tccgactttc ccagtaaagc catactgacg
    16201 caatactgta atagcttgtt ttaattggtc ggcatcattg acaaaaggaa agataacaga
    16261 tagattgggg ttggtttgat aaacttctgt aacgacatgt gcttcagcct gaaattcatc
    16321 caaacacgcc agtaaacgcc tggttcctct atagccaaac aagggatgcc cttcgtcaaa
    16381 aaactcttta gtccccacta aacaattggc ttctgtattc gttaattcag taaaacgata
    16441 ccaaacttct tcatctgagt acagggagca gatagtctct agataatctt ttacaaattg
    16501 ctgacaactt gctaatatga tgttttgatt aagctctctc aacaaatatt ccccacgaat
    16561 catgccgacg tggtgaaata gttgaggata aattttttca agaatttttt cgccactaag
    16621 ggcaagttga tttctcatag ccattcacct cgtcttaaaa gatacaatat catatataat
    16681 tatatcaaaa tttcctctag ttcaaaagct tcataaggtt cagataactc atatcctaaa
    16741 aattctgcta cttttttgga agcctcatta tgagcatccc aaagtggaaa catatctcta
    16801 tttaaactct ctagaatcat tgcagcacca agctttttag caaatccatt tccttgttcg
    16861 tttggtctag ttgcaacttc cacttcaact gctttacggt aaactaaccc tgaggaaatc
    16921 ccagcaatca gttcattatt tttaagaatc acaaagccaa atccaccttt taaaacaaaa
    16981 tcctgatagg actcaaaatc cccctgtaaa tcctgtgacc attcttccgt agaaaagcag
    17041 ttataaatat gattatcaat aggcacaata ttgtaacctt cctctaaatg agtaactaga
    17101 ttatttaaaa attcaacttg aaaatttgcc ttatctttaa aagaatagcg agtaaaagaa
    17161 tttagcccca cttttcgatg cagaaaatct tgccaattag tattttctga aataataatc
    17221 ttataatcta aaccatattt tctaacaaaa tcttcccaaa atctggaatc caactcccct
    17281 gctaggtata gaaaattccc aacatcataa aagcttgttg tcctatcatt gtttcggtag
    17341 acagttccaa tttgagattt aagaccatag agaaccatat ttttcttcca tgttttaaat
    17401 agttccatta tccctctact tcaccttcca attcatgtaa gaagtcttgt ccagttctgg
    17461 aaatcctaat aattcagact taaccttcaa gactaatggc gatgcatttt cttctgtaat
    17521 ctcttgaata tccatccaaa tatatccaag tgaatcattc gcaccatcag acacagcttc
    17581 cgaagtcgta acttgaggtg cactctcatt catttcaaca tcatacaagg ccatgacatg
    17641 gtcaaccata aaatttgtta actcttctct gacgaaaaca tcgtagattc gaggattaga
    17701 gtagcttcta acagtaaatc ccgtctcttc cataacttct ctagtcagcg tttccgtcag
    17761 tccttcacca agttgctgac tgcctccagg tagatcatac cgatgttgat aagggcctct
    17821 cgttttttca atgcagagta acttcccatt ttctaagcaa acagcgtaga ctccaaagtg
    17881 ttttttgatt tccatccaac tcctcctact tcaaagacca gccaccatct attgtcaaga
    17941 tttgtccttg catggcgctc gcttttccac ttgctaaaaa aagactaagc tctgctattt
    18001 cctctggctc aatccagcgc ttgattggag tttcactagc cacccagtca gccaaaccac
    18061 ctggttcaaa atccgcagcg gtcatagctg tcttgactgc tcctggagcg ataccaaaga
    18121 cctgaatccc agcttcagca tagtctagag ccaactgctt ggtgaagcca gccaaggcat
    18181 gcttggatga agtataggcg tgaccacctc cacctgctag gctagaagca atggaacaca
    18241 tattgatgat gattcccttt ttattttcca gcatttgtgt caaataatac cgagtcaact
    18301 ctactggaat aatgtagttg atttcaaaaa tctcttgaat gtcctgcgcc gtttgttcca
    18361 acagtggttt gtaatcatcc aaaactccag cagtattaca caaaacatcc acctgagggc
    18421 accagtcaaa aataggttcc aagtccaagg tcaaatctct ctgtaaaaag tgaaaatcac
    18481 cctctaagag tggcttttca ccttggtcaa ctccataaac ttgatagccc ttctctaaaa
    18541 agaggcgagc ttgagccaat ccgatccctg aactcactcc tgtaatgagt acacgtttag
    18601 tcatgcactt ctacccaatc cgttgccaaa acatcacaaa ctgtcgggct ccacatggaa
    18661 aaaccttctc cttcgccaga aacgttgatt aggaaataag gtgtcatttc aagtgcaagc
    18721 ccattttgct cgatggtatc aaagagttgg acatagtttt ccgcacctcc ccaaccagtt
    18781 cgtacatatt ttctcttagc ctttaaccca ggcaggatct cttcaaatgt catgtttttc
    18841 tcctttaatt ctacattctt catttaatta tagcaaaaaa ccgctttata cggctttttg
    18901 aatgtgagtt attcaaacct gctactactt acggcaaatt attccctgca gcaagataaa
    18961 tttcatacca ttcttttctt gttaagctaa agtttgccgc tcggctaact tctctcaagt
    19021 gcttaggatt tgttgtacct acgactgcct gcatttttgc tggataacgc aatatccaag
    19081 aaatggcaat agttgaagag gttactccat atttaatagc taaacgatca agtacttgat
    19141 ttaaagcttg aaatttctca tttccaacaa aattcccttt aaaatacccg aattgtaaga
    19201 cagaccatgc ttgaatgacc acatcgtgta attggcaata ttcaaaaatg ctgccatctc
    19261 gcatagctgc ttgactatct tccatattaa catgaaaagc tgattcaaat cctggagtaa
    19321 aagccgcact caattgtagc tgattaacag ctaacggctg cttgacatct tttttaagca
    19381 actccatcat cataggattt tgattagaaa ctccaaaatc tcgaacttta ccttgtttat
    19441 aaaggagatt aaaggcttct gctacttggt cagattccat caaagcatct ggtcgatgaa
    19501 ggagcaagct atctagatga tcaatcttca atctttgcaa aataccgtct actgatttta
    19561 taatatagtc cttagaaaaa tcaaaatagg taaattcttc aatgcgaatg ccacatttgg
    19621 actgaatcca catcttttct cttaaatctg gacgattttt taggacaaga cctaacagtt
    19681 cttcacaacg accacgacca taaatatcag ccaagtcgaa ggcattgatt ccaacagaaa
    19741 gtgctgtttc tacaagctct tcaacttctt ttacagactt atcttttatt ctcatcattc
    19801 cgagaacaat ttctgataat tctttgtcat cttgaccaag agttatgtat ctcatcaaat
    19861 ttttctcctt taatttctaa cattcttccc ttcattataa caaaaaaccg ctttgcaacg
    19921 gctttttgac tatacttcac tccattttat cttcttaaac ccacggaaca agacaaagat
    19981 tccaataaag aggacagcta aaggaataac ttttgtaagg aaaacatttg aaattcccat
    20041 ccactcataa taacggagca gagaacccac cacaagatgg gcaataatca tactgacaaa
    20101 tggacgaaag accgcttctt tccaattcca aataccgata actagcgaaa tcgtaaagac
    20161 agacaaacta tcccagggag ccggaatata aaaggctcct tcttgtatga agcttgccat
    20221 tcctacatat cctaaaacaa ctagaagaac tatagtccca acaacaatgt aagtgccaat
    20281 tttcatttta ggagaatctt ggactaaact tcttcgtaaa attgtggcca caagtccaaa
    20341 tccaatcaga aaaataagaa gttgccctaa aaatgtgagc aaattgactg ttaagagagg
    20401 acctttagaa aaatcactta gtagttgata ataacgtaat acagccagga caagaattgg
    20461 cgtcaaaagg gactctttga tagaactgcg aggtgctccc ttgagaatct ctttcattat
    20521 ttttttagga ttcttaccta gataatcctc tgcactcatg ccatctcgtt ctgcttctga
    20581 gaaatctagc atcatcaaat agatctgctc tctgagatag tcttcatcat agagaaatcc
    20641 agcaagatta aaactttccc acaactcctc aaaatacttt tgattctcct cagaaaactc
    20701 atgtagcaaa gcgcttgttt cttcgtaata cttcattttc ttcatggttt aacccccatt
    20761 cttaatccct tctacttttt gactcaaatc gtcccattgc tgccaaaaga ctgagacacg
    20821 ctcttctcct tctttcgtta atgaaaaata cttccgatct ggaccatctg gcgacgggcg
    20881 catgtcgcct cttatccatt gatttttttc taacttttgc aacaaaggat aaatagttcc
    20941 tggaacgata gtatcaaatc cagcctctcg caaagtctga accaactcat aaccataccg
    21001 ctctttttga ccaatcatat ccaagacaca accttcaaga acacctttta atagctgagt
    21061 ttctttcatc acttctccct tctaatctat tttgtaatac ctactagtaa cttcacctat
    21121 agtatattac ttctacacta gtttgtaaag cataatagtt aatactcttc gaaaatctct
    21181 tcaaaccacg tcagcgtcgc cctaccgtat gtatggtt
//
LOCUS       DAVBCW010000023        20534 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVBCW010000023 DAVBCW010000000
VERSION     DAVBCW010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20534
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.023"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(106..1785)
                     /locus_tag="VQ514_000765"
     CDS             complement(106..1785)
                     /locus_tag="VQ514_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET2499555.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLRLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(1787..2020)
                     /locus_tag="VQ514_000766"
     CDS             complement(1787..2020)
                     /locus_tag="VQ514_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HET2499556.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            2402..2623
                     /locus_tag="VQ514_000767"
     CDS             2402..2623
                     /locus_tag="VQ514_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499557.1"
                     /translation="MTNQVLEENYLCDSLLAFSPPRDLCRLVCILLWIKCLDALLMLG
                     FFVWGCRDSRFTTRKFHPIYQDEVVFAFF"
     gene            complement(2836..2988)
                     /gene="cibB"
                     /locus_tag="VQ514_000768"
     CDS             complement(2836..2988)
                     /gene="cibB"
                     /locus_tag="VQ514_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HET2499558.1"
                     /translation="MMKDLNNCREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(2990..3175)
                     /gene="cibA"
                     /locus_tag="VQ514_000769"
     CDS             complement(2990..3175)
                     /gene="cibA"
                     /locus_tag="VQ514_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HET2499559.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            3353..4036
                     /gene="tsaB"
                     /locus_tag="VQ514_000770"
     CDS             3353..4036
                     /gene="tsaB"
                     /locus_tag="VQ514_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HET2499560.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMESLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLTHTLNIELVGMSSLLALVPY
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMPEAHLSFEEVLEKVKGASQVTFVGEVGPF
                     VEQIQEHLPRTNYKETLPNAANLALLAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            4033..4470
                     /gene="rimI"
                     /locus_tag="VQ514_000771"
     CDS             4033..4470
                     /gene="rimI"
                     /locus_tag="VQ514_000771"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HET2499561.1"
                     /translation="MIEIKRIQQQPDLAQAIYAVMAAVYLVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQENLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RKSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            4460..5470
                     /gene="tsaD"
                     /locus_tag="VQ514_000772"
     CDS             4460..5470
                     /gene="tsaD"
                     /locus_tag="VQ514_000772"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HET2499562.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLYHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(5516..6816)
                     /locus_tag="VQ514_000773"
                     /pseudo
     CDS             complement(5516..6816)
                     /locus_tag="VQ514_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(7092..7443)
                     /locus_tag="VQ514_000774"
                     /pseudo
     CDS             complement(7092..7443)
                     /locus_tag="VQ514_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7550..7842)
                     /locus_tag="VQ514_000775"
                     /pseudo
     CDS             complement(7550..7842)
                     /locus_tag="VQ514_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            8085..9291
                     /locus_tag="VQ514_000776"
                     /pseudo
     CDS             8085..9291
                     /locus_tag="VQ514_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            9284..9932
                     /locus_tag="VQ514_000777"
                     /pseudo
     CDS             9284..9932
                     /locus_tag="VQ514_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            9942..11558
                     /locus_tag="VQ514_000778"
     CDS             9942..11558
                     /locus_tag="VQ514_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2499563.1"
                     /translation="MYTIIKSNIKKFSLLTIFIVAGQLLLIYAATINALVLNELIAMN
                     LERFLKLSIYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHS
                     KSSGTYLSWLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLM
                     IMLLVPKIFASKMREVSLNLTNQNEAFLKSSETILNGFDVLASLNLLYVLPKKIKEAG
                     ILLKMVIQRKTTVETLAGAISFFLNIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTG
                     VIFTALGELGGQLSSIIGTKPIFLKLYSINPIESNKMNDIEPNEVNRDFPLYEAKNIC
                     YKYGDKEILKNLNFCFQRNEKYLILGESGSGKSTLLKLLSGFLRDYSGELRFCGDDIK
                     KTSYLNMVSNVLYVDQKAYLFEGTIRDNILLEENYTDEEILQSLEQVGLSVKDFPNNI
                     LDYYVGDDGRLLSGGQKQKITLARGLIRNKKIVLIDEGTSAIDRRTSLAIERKILDRE
                     DLTVIIVTHAPHPELKQYFTKIYQFPKDFI"
     gene            11578..12422
                     /locus_tag="VQ514_000779"
                     /pseudo
     CDS             11578..12422
                     /locus_tag="VQ514_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813546.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12490..13991
                     /locus_tag="VQ514_000780"
                     /pseudo
     CDS             12490..13991
                     /locus_tag="VQ514_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819023.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase"
     gene            14003..14752
                     /locus_tag="VQ514_000781"
     CDS             14003..14752
                     /locus_tag="VQ514_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HET2499564.1"
                     /translation="MSDNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSKLKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFPYVSDSSMVSYMGKTKELKMYQEELDEKHYIDRISSILCYKSQILI
                     IWKSVEKLLNNIKELYLRNGAAYSIRFWIKK"
     gene            14765..15937
                     /locus_tag="VQ514_000782"
     CDS             14765..15937
                     /locus_tag="VQ514_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HET2499565.1"
                     /translation="MNIAVIGLGHVGLAYALLFASKYKVVAYDIDSVKINNLKKGTLP
                     SKNEELMKFFCENNLNITFFDTFSEIKNNIDYYIIALPTDYDEKIGSFNTYEIEQTVS
                     KILRVKPNGKIILKSTVPIGFSNKLKRLFDTKNIIFVPEFLREGCSIYDNLYPSRIVV
                     GDETVEGRKIAELFLSISTHSTANIKNVMLVSPTEAEAIKLFSNTFLALRVAFFNELD
                     SFAERRSLNAEVVIKGVCLDPRIGNFYNNPSFGFGGYCLPKDTRQLKKEFIEINAPVI
                     EAIDISNTNRKQFIVKQILERKPKIVGIYKLGMKYNSDNYKESAILSIINELLIVGIK
                     ILVYEPNLNVSIDNVIFEKNFELFIKQSDLIVANRWDRGLEAYKDKVYTRGIWIRD"
     gene            16332..17195
                     /locus_tag="VQ514_000783"
     CDS             16332..17195
                     /locus_tag="VQ514_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HET2499566.1"
                     /translation="MIEKMELGEFYKELRLARKLKQSDVACAGLTASQLSKFELGQSM
                     LSADKLILAIQGINMNFDEFGHKLNNYQESPHMRIGRRVVDCFSHQDIAALEQLLEEV
                     DQEQMAQTYRRLNAIVIKNALHSLDKSYPLTEEDSEFLTTYLYAIESWTWFELYLFCN
                     TMPFLSNQDLIFLSTSLLEKSKEFKELVHNRWYMKSGYLNIISELMERKLFSYIPIFE
                     AELERMLRPYDVFEKVLWQFLKKMQIFLQTKGSNQKEIEHFIQSLQVLENPQLTALFE
                     LRLQQYKELID"
     gene            17481..17714
                     /locus_tag="VQ514_000784"
     CDS             17481..17714
                     /locus_tag="VQ514_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499567.1"
                     /translation="MLNLQFAETMELTEAELQDVRGGNLVNSMGGGGRSGISGWGVPG
                     IYPGWGNQGMSPNRGAFDWTIDLADGLFGRRRR"
     gene            17736..18413
                     /locus_tag="VQ514_000785"
     CDS             17736..18413
                     /locus_tag="VQ514_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2499568.1"
                     /translation="MKKMKEVKFHLATGLLILTYYLIFNVTSDLDFMVALSDNMYYVF
                     QVLLVLILGTIATIAFVKSEHWKECGRFQFRWSYLGVFLLSFFLLFVWANLTTYIFPR
                     TQNGSTVVEVATSLTGISYFVTRILYTSIIAPVSEEVVCRGLLMTSLSKVKRYYLDVL
                     VSAAIFGAMHVLQYGWITTDFIKYFGMGLIFCMMFRYTRSIYWAIALHASWNSFLLIV
                     TLLVFGY"
     gene            18425..19090
                     /locus_tag="VQ514_000786"
     CDS             18425..19090
                     /locus_tag="VQ514_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2499569.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVAGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVAWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEDMIYRGLVMTALEKGKKWGLDVL
                     GSAILFGVSHISNHGWVLTDFFVYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            19162..20388
                     /locus_tag="VQ514_000787"
     CDS             19162..20388
                     /locus_tag="VQ514_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2499570.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTVISFTKFLLYSIVSLSLSGQSFSLL
                     LVTFICLLNLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTISYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPISTLFLRNQPFLNLQTGQSLALLATLEL
                     SALILGSLMSGYLQHTISIKIALYASLLLQLLLLVGFATAHFGLILIFSFLDAFFAGI
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALGTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
BASE COUNT         6424 a         3215 c         4157 g         6738 t
ORIGIN      
        1 gaactgacga agtcagctca aaacacagtt ttgaggttgt ggatagaact gacgaagtca
       61 gtaacatata tacggcaagg cgaagctgac gtggtttgaa gagtattatt cttcatctgg
      121 tgtgaggatc atcgggatga tgatcggttc acgttcggta ttttcataga ggaaggggcg
      181 aatagcgttg acaatggcac cattgacaga ttgcacgcta gcatccttat ttttcagtgc
      241 gatacgaatg gcattgaaga ggatacgctg gctttggcga atcaagtctc cagactctct
      301 catgtagaca aagcctcggc tgaggatatc tgggccagac agaatcatct gcgatttgaa
      361 gtcaacagtt gcgactgcca gaacgacacc gtcttcagat agatcgcgac gatctttgag
      421 gacagctgcg ccaatttcac cgatacgatt tccatcgaca tagatatctt gggcgttgaa
      481 atgacctgcg atacgagctg agtcagcagt aagggcaagc acatcgccat tgctcatgat
      541 aaagatattg tctttctcaa caccagtatc cactgctagt ccagcgtgga ctttttgcat
      601 gcggtattca ccgtggacag gcatgaagta ttttggctta atcaagcgga gcatgagttt
      661 ttgctcttgc tgaccaccgt gtccagatgt atggatattg ttcactttac cgtggataac
      721 ttcgacacca gcttcagaaa tgatgttaat cagcttgttg acgctagtag tgtttccagg
      781 gatgggacta gaagagaaga taacggtatc acctggttgt aattgtactt gacggtgggt
      841 tccgttggcg atacgagaga gggctgccat aggctcaccc tgactacctg tacagaggat
      901 aagaacttct cctgcaggat aatctttgat ttcatttggc tcgataaagg ttcccttagg
      961 agctttgatg tagccaagat cgattccgtt gacaatggcc ttttccatag aacgaccaaa
     1021 gaccgcaatc ttgcgtccag tcttaacagc agcttctgtt gcctgctgga gacggaagat
     1081 atttgaggca aaggatgcaa agatgatacg tccttcaata ccttggataa tcttcataat
     1141 ggactgacca acgacttttt cagagttggt aaaggttggt acttccgcat ttgtcgagtc
     1201 agacaggaga cagagcacgc cttcttcacc aagcgcagcc atacgatgca agtccgcagg
     1261 ttctccaact ggagtaaagt cgaacttaaa gtcacccgta cagacgattt tcccttgagg
     1321 agtatgaatg acaatcccca aaggctctgg aatagagtga gtcgttctaa agaaagttgc
     1381 cttgagattt ttaaaggtca actcggtgtt gtggttgatt tcgtaaagtt tggcgttgcg
     1441 caagaggccg tgttcttcga gtttcccacg gatcaaagcc aaggcaagcg gtccagcgta
     1501 gatagggaca tttgcttgct tgagtaggaa cggaatccca ccaatgtggt cctcgtgtcc
     1561 gtgtgtgatt aaaacagcct tgacgcggtc gatattgtct acgatgtaag agtagtcagg
     1621 aatgacatag tcgataccaa gcaagtcatc ttctgggaat ttaatcccag catcgacgat
     1681 gataatctcg tcttggtatt caattccgta agtgtttttc ccgatttctc ctagaccacc
     1741 gatggcaaaa acaccgactt cttcaggttt aagagtgtag gccatattag aactccgtaa
     1801 tttcgaaggc gccagtttct ttttcgtaat cgagcaattt gtcagacaag agttcgatat
     1861 attcgatatt gtactctggg cgattttctt cgacaagttg gcgagcagtg atacggccct
     1921 caagttctga gctggcatcg atgtctaggt aaagcgcgcg tgttgtttca cggcgtgggc
     1981 tacgttcttt tgtttcttga taaaaaactt tgtaaatcat atagttcctt tctatttaca
     2041 ggtcagctta ttccaaactt ttagcagata tttgcttgat ttcattccat tttgtgtcta
     2101 gattgacctt gattcgttac aattatttca attataccac aaaatgaaaa attagtaaaa
     2161 gacggaaacg agaaagttag gggacgattt cttcggtaag cgtttgcaaa aacaaagaaa
     2221 atgtgttatg ttgataacgg aagtgaagtt tattacttgt cataattcca tatagatttt
     2281 aagtcaagga ggttgcccta tgtataactt atttgctttt gctatcggag ttcgtttgct
     2341 agcttttatc gtttttgttt tgcgtttatg gaggaaatct tctatttgcc aaactggaac
     2401 gatgacaaac caagttcttg aggaaaatta cctttgtgac tctctactcg ctttttctcc
     2461 tcctcgtgac ctatgtcgtt tggtttgtat tttactttgg attaaatgct tagatgccct
     2521 gcttatgctg gggttttttg tttggggatg tagagatagt agatttacga cacgaaaatt
     2581 ccaccccatc tatcaagatg aggtggtctt tgcatttttc taaaatgata tgacaatgga
     2641 attttcaaag cttattattt ttatgggagt cattgttctg ctcaaaaata gagttgaagc
     2701 ataaaaataa aacaatacaa acagataaat cgaatggtaa agggatggta gtaatgttca
     2761 aatcattgag aatgctgagc aaactgtaag agatgaagac aataaaattt atccttctaa
     2821 aaatgtgttt cttatctagc atgacttacc aaacttttta cgaaggtctt ttccaatggt
     2881 ataacctgcc ataaataaag cgataccaac acctacacca aagccaccct tgatttcttg
     2941 caattcctta ttagaaattt cacgacagtt gttcaaatct ttcatcatat taatctcctc
     3001 ctaaaaaata accagctgcc aaaccagaac ctataagtgc tgttccacct gcaatagctt
     3061 tccaagatac tgctactcca aagataacca agggagcgag accgccatca atatctgata
     3121 attcattttc agataaggat aaaaattgat tgtcaagaat gtcaaaattt gtcattgaac
     3181 tttcctcctt ttttcttgtt cacttttata ttcggaaaag aaaaggaaaa gtgagcattt
     3241 ttttaaatta tttttagtta agtgagggca ctcttgtctg ggggcaaaga aaattcccat
     3301 gtgccatctt ttgtagtata atagtaagca gacaaaaaga taggaatgtg ttatgaaagt
     3361 attagctttt gatacgtcca gcaaggctct ttctctcgct attttagagg ataagcaggt
     3421 tcttgccgag acgacgatta atatcaagaa aaatcacagt attaccctca tgccagccat
     3481 tgattttttg atggagagtt tggactggac gcccaaggat ttggaccgaa tcgtggtggc
     3541 ggaagggcca ggtagctaca caggcttgcg aatcgcggta gccactgcca agaccttgac
     3601 tcatactctg aacattgagt tagttggtat gtctagcctc ttggctctgg tgccatatca
     3661 acaagaaggc ttgttcgttc ctttgatgga tgcgcgtcgc aacaatgttt atgcaggatt
     3721 ttatgaaaat gctaaacctg tcatgccaga agcgcaccta tcttttgaag aggtgctaga
     3781 aaaagtcaag ggtgctagtc aggtaacctt tgtcggagaa gttggcccct ttgtggagca
     3841 gattcaagaa cacttgccaa ggactaatta taaagaaacc ttgcctaatg cagccaatct
     3901 tgctctttta gcttgggaca aggaagcaga ctccttgcat gattttgtgc caaattacct
     3961 caaacgagtc gaggctgagg aaaactggct caagaaccat accgagtctg gtgagtccta
     4021 cattaaacgc ctatgataga aatcaagcga attcaacaac agcctgacct agctcaagcc
     4081 atctacgctg ttatggcagc tgtttaccta gtcagtcctt ggactctgga gcaaatccaa
     4141 gcagatctgt cccaagacca gacttggtat gcattggctt atgatggggc agaagtgatt
     4201 ggatttctag ctgtgcagga gaatcttttt gaagcagaag tcctgcaaat cgctgtcaaa
     4261 ggagcttatc agggtcaggg aattgcgtca gccttgtttg ctcaattgcc gacagataag
     4321 gagattttcc tcgaagtcag aaagtcaaat caacgagcgc aagcatttta caagaaagaa
     4381 aaaatggcgg tcatcgctga gcgaaaggcc tactaccatg acccagtcga ggacgccatt
     4441 atcatgaaga gagaaataga tgaaggatag atatatttta gcatttgaga catcctgtga
     4501 tgagaccagt gtcgccgtct tgaaaaacga cgatgagctc ttgtccaatg tcattgctag
     4561 tcaaattgag agtcacaaac gttttggtgg cgtagtgccc gaagtagcca gtcgtcacca
     4621 tgtcgaggtc attacagcct gtatcgagga ggcattggca gaagcaggga ttaccgaaga
     4681 ggacgtgaca gctgttgcgg ttacctacgg accaggcttg gtcggagcct tgctagttgg
     4741 tttgtcagct gccaaggcct ttgcttgggc tcacggactt ccactgattc ctgttaatca
     4801 catggctggg cacctcatgg cagctcagag tgtggagcct ttggagtttc ccttgctagc
     4861 cctcttggtc agcggcggac acacagagtt ggtttatgtt tcggaggcag gagattataa
     4921 gattgttggg gaaacccgtg atgatgcggt tggtgaggct tatgataagg tcggccgtgt
     4981 catgggcttg acctatcctg caggtcgtga gattgacgag ctggctcatc aggggcagga
     5041 tatttatgat ttcccccgtg ccatgattaa ggaagataat ctggagttct ccttctcagg
     5101 tttgaaatct gcctttatca atctttatca caatgccgag caaaagggag aaagcctgtc
     5161 tacagaagat ttgtgtgctt ccttccaagc agcagttatg gacattctca tggcaaaaac
     5221 caagaaggct ttggagaaat atcctgttaa aaccctggtt gtggcaggtg gtgtggcagc
     5281 caataaaggt ctcagagaac gcctagcagc cgaaatcaca gatgtcaagg ttatcatccc
     5341 ccctctgcga ctctgcggag acaatgcagg tatgattgcc tatgccagcg tcagcgagtg
     5401 gaacaaagaa aacttcgcag gctgggacct caatgccaaa ccaagtcttg cctttgatac
     5461 catggaataa agagtggctc tttgtcaagt gtagtgggta gtcgaaaagc tgcaatctgg
     5521 agagtacgaa attcgtactc tcttttttga tgttcaaagt catcaaaatc cgttttttaa
     5581 aattgttaaa gttccggaaa ccaaaggctt tgcgcttgat aatcttaatc aaattattgg
     5641 tcgcttcaag tttagcgttg gaataaggca gcttaagtgc gttgataatc ttatctctat
     5701 cccttaaaaa agtccaaaag accgtttgaa aaagcggatg aaccgttggc agttcttgct
     5761 caatcaatcc aaagaaatgg tcggcattct tctcttgaaa atgaaagagc aggagttgat
     5821 agagttcgta gtgaacctgt aatccctcag agtaagaaag cagcttatct cgtacttccc
     5881 gatgggttaa gtgcattcga aacgtgggct gatagaatcg tttctgacag agcttgaagc
     5941 tatactggtg tagtaaatca gtccagtgga ctgatttagc ccgagcctag aaacgaaaaa
     6001 gcgagggttc caaaagcgct tgagcgcccg atactccaag gattttcggt caaattggtt
     6061 catgatttgg attcgtactc ggttcatagc tcggctcaga tgttggacaa tgtggaagcg
     6121 gtcaagaaca atcttcgcct ttggaaagag ttgcttagcg agccgataat aagggctgta
     6181 catgtccatg gtgatgacct cgaccagctc ccgaacctct ctctgatagc gttggaaatg
     6241 gtttcgaatc accgcatgag ttcgcccgtc taaaattgcg aggatggatt tggactcaaa
     6301 atcttgggca atgaagctca ttttgctctt tttgaagcta tactcatccc aactcataac
     6361 ttttggcaac ttggtccaat cggtttcaaa cttaaattcc ctcagttttc ggatgacggt
     6421 ggaagttgag accgccaatc tgtgggcaat ctcagtcata gactgctttt caaggaggag
     6481 ctgagcgatt ttttgtctca ccatgttgga aatctggcaa tttttcttga caatggatgt
     6541 ttgagaaacg accaccttaa ggcaattctt gcactgaaag cggcgctttt tgagatgcag
     6601 taccgtgggt aaaccctgac agtcgagaag cggaatttta gaggctttct ggaagtcgta
     6661 tttgatcatc ttcccttgac aatgaggaca aggaggagcg ggggaatcca actttgcctg
     6721 aacgactaga tgggtttggt atttcagaac agacaagatt ttgatatttt tgtcttccaa
     6781 tccgagcaaa tctgtggtat tcttaagttg ttccatatgg accctttcta atgagttggt
     6841 tagacgcttt tcattatagg tcatatgggg ctttttttct acaagaaacg accctataat
     6901 tcctggggtg ggattaccca ctacagaaat tatagagcca aagcattcca aagtcttgtc
     6961 tgactctgga atgttttctg ttcacagttg ttttgtaact gcctaataag taaccgcgct
     7021 ataacgaggt atctaccgag attgtcaatt ctccagtata ctgttataaa aaacaaaact
     7081 ggaggatttt tttatgtcct cccatcgtcc catcgactgt tccgtagctg cccaataacg
     7141 gagtatagtc ggctgaaaca agaataggac aaaagtgcct caagaaaagc atcgtaattt
     7201 gacaatatta ttctgagatg tttttgatgt aagcccatat tttctcaatg ggattatact
     7261 cgggtgaata ggtaggaagt ggtaacagtc tatgccctgc tccttacaca tgttcattct
     7321 gtgaaacctt gcattgtcca taatgataag ggatggtgta tctaaagtag gtagtaagaa
     7381 tttttggaag caagcttcga aaaagtcact cgtcatcgta tctttatagg tcatcggagc
     7441 catagtatat tgaaacaaaa aagggcatta gtacttctat aacagtatcg caatctctgg
     7501 tgtgcgttgt ctcgatctta attcaaataa ttatatcttc ttccagagag actaactgcc
     7561 tgctcaaggc tcgatcatat ttgagataaa aataggtctc aacccctgtc tcataaatat
     7621 aaataggagt caggtagctt aagtgattca attctttaag gaaccgattt actttttctg
     7681 ggtcttgttc gcagtaggta cggctctttt tagactatat cccatagcct ttagagcgta
     7741 atgaatagtt gttggaggac agttgaattc agcagctatt tcagtcaaat aagcatcggg
     7801 atgagtttca atatagatct ttaatttatc tctatcaact ttttcaatta atagtggaag
     7861 ttttcttaaa gttagtgtgt tgaaacgttt ttatacagtg ctaaaatttg aagtgtagtg
     7921 cagatgtatt tgtaatagta ttatagcaat tctttggaga aggtcgagac aagagaaaat
     7981 aggtatcttt gagatataag tatgatttac tataaaatta ttgagttatt cataaaaatt
     8041 tctctatcaa atatcataat gcgtcgttta aggagatggt tacaatgcaa tatagttgtg
     8101 gaaaaataaa tattaatatc cctgatggat atggagacat taaggatata gtgttttcgg
     8161 ctcatattat agttaggtat aataatggac attgtggagg tatagatccg catataattg
     8221 gactttgtaa aaagcagatc agaaggatgt ctccatatcc tattttgata atcgtatcta
     8281 gagattcaaa ggttattgat gattataaaa atttagatat tgcctatgtt gattgtactc
     8341 aatgttcaaa taattttgaa actgccttac acgttaaaaa tattttaaaa ttattaaaaa
     8401 tccaacttat acactgtcat ggctactcaa ctaattattt tttatatatg ttaaaaaaac
     8461 ttgacaagaa tggatttgga aaggttaaga cagtgataac atgccatgga tgggttgaat
     8521 ataacttaaa aaagaaattc ttgacttatt ttgatttttg gacatattct atgggagatg
     8581 cttttatttg tgtatctgaa actatgaaaa aaagattgga gagtataata aaaaaataag
     8641 aagatagttg caataaataa cggaattaat gtatcaaata gcgatttaga tgtagtaggt
     8701 gttcaagact ttaaaaaaga attttgtatt ccaaataaca aaaaaatcat ttgttatgtt
     8761 ggaaggttgg atccagaaaa aaggccggat agattccttg aatttgcaga aaaattattt
     8821 ttagtcagag aagatgtgat ttttattatg gctggaaatg gtagtatgtg ggctactttg
     8881 aaagaaaaaa tttgccattt gaaatgtaga gataatttca ggttacttgg tgaaatctat
     8941 ccagctacta tagtttacca aatatcggat ttattgtata ttccttctga tacagaagga
     9001 atacctatgt gtgttttaga gtcaatgtca caagaaactc cggttttagc tagtaatgtt
     9061 ggcgggttaa gtgaaattat tgaacatagg gttgatggat ttttatttga gaaggaagat
     9121 gttgagggag tgtgtgcttg tgctaatttt ttactcaatg attctgagta tttgaaatat
     9181 ataggtgaga atagtaaatc aaaaataaga aaacattttt ctgtgcaaaa aatgtttgta
     9241 gaaaccatga gagtatatga tgaattatta gagaagagta gtcatggata ggaatataga
     9301 tcaagaatta gtaagtatta taataccaac acataatagg tatgaatctc tgattagagc
     9361 tgtgaaaagt tgtttacatc aaagttataa aaatatcgaa gttataatta ttgatgataa
     9421 ttatagtaat gtaaatttaa gaaataaaat tatccatcaa tttggctata ccagtcatag
     9481 aattaagtta attttaagta atgaagattt aggtgcaact aatgccagaa acataggtat
     9541 caaaaattct agaggtaagt atatatcatt tttagacgat gatgatgaat atatgccaga
     9601 tcgaatttta aagttgatgg cttgttttaa aaagagtaga atgaagaatt tagctttagt
     9661 ttatagttat ggaataataa tttatccaat aggaaatatt ttaatttatt gagtttcaac
     9721 gagagaatat tggttacaaa gaaatattat tctttaaaca taaagcggtt actattaata
     9781 ggagacaaat gcaaggcttt aaaagtaatc aagaaggcaa gagaagaaaa aatttttaac
     9841 gaatttcttt ttcgaaatat atgttattat atcattttgg ttttttctat tgtatatatg
     9901 acaactatgt tcaattaaaa tttagaaagt gagaaactat tgtgtatact attataaaat
     9961 caaatataaa aaaatttagt ttattaacga tatttattgt tgctggtcaa ttattgctaa
    10021 tttatgcagc aactattaat gctctggtgt tgaatgaatt aattgcgatg aatttagagc
    10081 ggtttttgaa attgtcaatc taccaaatga ttgtctggtg tgggataata ttccttgact
    10141 gggtagtgaa aaattatcag gttgaagtga tccaagagtt taatctagag attcgaaata
    10201 gagttgccac agacatctct aactctacct atcaagaatt tcatagtaaa tcatcaggaa
    10261 catatctttc gtggctaaat aatgatgttc agactttaaa tgatcaggct tttaaacaac
    10321 tttttttagt aataaaagga atttctggta ctatatttgc agttgtgact cttaatcact
    10381 atcattggtc attgactgta gccaccttgt tttcattaat gattatgcta cttgtaccaa
    10441 aaatctttgc atcgaaaatg cgagaagtta gtctaaattt aactaaccaa aatgaagctt
    10501 ttttaaaatc tagtgagact atattgaatg gatttgatgt gttagcgtcc ttgaatcttt
    10561 tatatgtatt gcctaagaaa attaaagaag caggaatttt attaaagatg gttatacaaa
    10621 gaaagacaac tgtagaaacg ttagcaggcg ctattagctt ctttctcaat attttttttc
    10681 agatatctct cgttttttta acaggctatc ttgcaataaa aggaatagtg aaaattggta
    10741 ctattgaagc aataggagca ctaacaggtg ttatttttac agcgctaggt gaattaggag
    10801 gtcaattatc ctctattatt ggtacgaagc ctattttttt aaaattgtat tcaattaatc
    10861 caattgagtc aaataaaatg aatgatatcg aaccaaatga ggtgaataga gattttccgt
    10921 tatatgaagc aaaaaatatt tgctataagt atggagataa agaaatatta aaaaacttaa
    10981 atttttgttt tcaacgtaat gaaaagtatt taattttagg tgaaagtgga agcgggaaat
    11041 ctacattatt aaaattattg agtggctttt tgagagatta tagtggagaa ttgcgattct
    11101 gcggggatga tataaaaaaa acctcctatt taaatatggt ttcgaatgtt ctatatgtag
    11161 atcaaaaagc ttatttgttt gaaggtacga ttagagataa tattttattg gaagaaaatt
    11221 atactgatga agaaatacta cagtctttag agcaagttgg tttgagtgta aaagattttc
    11281 ctaataacat tttagattat tatgttggtg atgatgggag attactgtca ggagggcaga
    11341 aacaaaaaat tactttagct agagggctaa ttagaaataa gaaaatagta ttaattgacg
    11401 agggaacttc tgctatcgat aggagaactt cgttagcgat tgaacgtaag atattagata
    11461 gagaggattt gactgtcatt attgttaccc atgctccgca tccggaactt aaacaatatt
    11521 ttactaagat atatcaattt ccaaaggatt ttatttaaga atatagggga gggaaacatg
    11581 agattctatt gtgaagctta tgaggtaatt actgaagaga aaaaagtata ctttaatgat
    11641 attgagttag aagttaagta ttcctctgtc gaagaattgt ttataatatg tgagaaatta
    11701 ttagataaga aaaaagtatc atttttttat gtatatgaga aacccttaag gtatctgtta
    11761 tttgactata tatttctact agttctagct aaaaaaaata tcccaatctt agatggagtt
    11821 tccaataagc aagttgatcc aacgttgctg catcactttt ctttggaaat tgaaaagaat
    11881 tttatcgatt tttgctataa aaatatggat ctcatcctaa aaactcaaag tatatctttg
    11941 tgtcatagag aagagcttat tattgtagat gtttcagatg actctaaatt tggtgttttt
    12001 tacaagcgtt ttagaacact ggttgataaa aataacggaa aatatgtttg tgtttataat
    12061 ttcagtcgtg tttcagatat actgcaaaac tggaaaaatt attgcaatcg aaaattttcg
    12121 gttacgttta ctgaaagcca atttgaatta tttaagcttt tatataatca aaaaaatttt
    12181 aaaacaattt cgttgctatt tggaaaaaaa ttgttgctgg aggaattatc tattatagcg
    12241 atcttactaa tatagaatac ttttgtattt tttggtggga tagtatgttt gggaaagatt
    12301 ctattggaaa atacgtatat gtagaagaga ttagtagatg tcatttttta gatagaaatt
    12361 attctttttg ttatggttta caagattata aaagtaaact tattaaatat tttttagaat
    12421 agttaatcta ttcgagagag ataaagaaat atggaaaata ctttattgaa tatcacagga
    12481 agcttcgata tggaaactag gaatctaatt agttattctt taactgatat ctttgaaaca
    12541 gataaaataa gaattgaact tcttggagaa atttattata aaaatataaa gttggaattg
    12601 catgaattcg ctggattgta taagatttat ggaattagct taataaagaa tattaccgga
    12661 atgtttttaa ttataatttt tgatacgaag acaaaagaat taaaaatttt tcaagatata
    12721 acgacttcat actttaattt atactatact gtttatggag gtgtgttcta tatatagtac
    12781 atctttaaag aaagtgatga agttatctca tgttcccgtt accttaaata acaaaaaaat
    12841 acaagaattt atgagaaatg gatttatttt agattcaaat actttagtaa ccgaaattaa
    12901 taaattagaa tatttctctt acatttctgt aaataatact cttcgtatat gtggtattga
    12961 ttataatgat tcaaacaatt ttactaaaga acaagtatta aagaattggg actctatgct
    13021 acgtgaatct atattaagag tgtatagtga agcaggagag gctaatatta cactatcatc
    13081 tggttttgat tcaaattata tattatatac attagcgaat tatacgaatt cttcaattaa
    13141 tgcattttgc ataggaggag aaaaaggaat taatgagatt cctgaagtaa ctaaaattgc
    13201 aaaattttat ggtataaatt tattagttga tactgtaatg agcccaagat ttagaatatt
    13261 ttcccgatat agtatggaga ttggaaggaa gtctctttga atgtggtgtc atactgcagt
    13321 actatttagg caggttgttg ttccaaaaag ggaacacaag tattctttgt ggcgagtcag
    13381 cggatgaaat aatgactttt aagtatcatt ctgtgaacta caatcaattt tgtaatgata
    13441 aacagaagtc agtatatttt agttattccg actatccatt ttatgttaca aattcaattg
    13501 ttttaaagaa aaatagccta cttttgcata gttttggcat tcatcctaga tatccatata
    13561 aaatgtctga gattgttgaa atgtctaaaa aaatatcaga tctaaacgat aaaaaagaat
    13621 ttcacaaaaa gaactgtgag ttgaggtttc aagatagtgt tcttgataac ataaatagtg
    13681 tcccgggtac aactcattta tttagttgtt taaatattca aacattagta aaaattattt
    13741 tatatatttt tcgatacaac agttgtatga aaatatttaa ttttagagat aaagagctta
    13801 tcttttttaa taagattgta aatgggctga tacaaaatat tgaggagaat ttagaggatg
    13861 atattgaaag gatattgaaa tatttatata tatgtttatt taacgaaata ttcattatta
    13921 agaataaggt gaattttttt gatgatgtgg aatttaatca gacacttagt gagtttttag
    13981 ataaattgta gagggaatgg ttatgagtga caatactaag tatatctttt tatctccaca
    14041 tttagatgac gcaatttttt cctgtggtga ttatatttca aaattaactt cagaaggaga
    14101 aattgtactt gttattacta tattttctgg ttaccctttg agccaacagc tacagccctc
    14161 tgctaagcaa tttcataagt tgtgtaacct tgggaaatat ccgattgaag aaaggaagaa
    14221 agaagacagg ttagcttgtg aacgtctaca atgtgatttt agacacttat cgtactatga
    14281 atgtctctat aggaaagata gaaatggcaa ttttctatac cgtcatatct atagtaaatt
    14341 aaaaaatgag gacacattaa agaatgatat cataaaagag ctgttaatgc atttagacga
    14401 caaatgtgtc gtatattgtc cgctttcact tggtgaccat gttgatcatg tttttgtgaa
    14461 tagtattgga agggcgcttg aatttatgcg ttataaagta atatattatg aggacttccc
    14521 atatgtaagt gatagtagta tggttagtta tatgggaaaa actaaagaat taaaaatgta
    14581 tcaagaggaa cttgatgaaa aacattatat tgatagaata tcatcaatat tgtgctataa
    14641 atctcagatt ttgataatct ggaaatcagt tgaaaaactt ttaaacaata ttaaagagtt
    14701 gtatttaagg aatggggcag cttattctat tagattttgg attaagaaat aaggaagtag
    14761 ttttatgaat atagcagtaa ttggtttggg gcatgttggg ctggcctacg cgttactatt
    14821 tgcatctaaa tataaagttg ttgcatatga tatagattct gtaaaaataa ataatttaaa
    14881 aaagggcact cttccatcta aaaatgaaga gcttatgaag tttttttgcg agaataactt
    14941 aaatattact ttttttgata cattttctga aattaaaaat aatattgatt attatattat
    15001 tgcgcttccg acagattatg atgagaaaat tggtagtttt aatacatatg aaatcgaaca
    15061 aacggtatcg aagattctga gggtaaaacc taatggaaag attattttaa agtcaacagt
    15121 tccgatcggc ttttcaaaca aattaaaaag gctgtttgat acaaaaaata tcatttttgt
    15181 ccctgaattt ttgagagaag gttgttctat atatgataat ttatatccaa gtcgcatagt
    15241 tgttggagat gagacagttg aaggaagaaa aattgcagag ttgttccttt cgattagtac
    15301 tcatagtact gccaatatta aaaatgttat gttagtttct cctactgaag cagaagcaat
    15361 taagcttttt tctaacacat tcttagctct ccgtgttgct ttttttaatg aactagattc
    15421 ttttgctgag aggagaagtt taaatgctga agttgtaata aaaggtgttt gtttagatcc
    15481 aagaattgga aatttttata ataatccttc ttttggattt ggaggatatt gtcttcccaa
    15541 agatactaga caattaaaaa aagaatttat agaaataaat gccccagtga tagaagcgat
    15601 tgatatttca aacacaaata gaaaacagtt catagttaaa caaatattgg aacgaaagcc
    15661 aaagatagta ggaatatata aattggggat gaaatataat tcagataatt acaaagagtc
    15721 agctatttta agtataatta atgaactatt gattgtcggt ataaaaattt tagtatatga
    15781 accaaattta aacgttagta tagataacgt aatttttgaa aagaattttg aactatttat
    15841 aaaacaaagt gatctaattg ttgcaaatag atgggatagg ggccttgaag cttataaaga
    15901 taaagtttac acaaggggga tctggataag agattaacta ttgatattaa aaaggcaata
    15961 atttataaaa tttgtacaaa aaaatgttaa attttcacat tttcatagta tactgtttaa
    16021 tagaaagtag gttctttgta accgctccat tataaaatcc tttcactctt ccggtatact
    16081 attaggaaaa acataactgg aggatatttt gagctcacac ctcatcgttc cagtggctgt
    16141 tccccaagct cgtgtactgc ccaaaaaagt tagacattct atttagaacg aggattgagt
    16201 tctgtattgt acaaggctcg gtccttttag agtcagctta aggctggctt tttcaatcac
    16261 caaagtgtca gaatgttttg acaaatgaac acaaataatg atataatatg caaaagctag
    16321 gaggtggtag gatgattgaa aaaatggaac tgggggaatt ttacaaggaa ttgcgcttgg
    16381 cgagaaagct caagcagtca gatgtggctt gtgctggact aacagcctct cagttgtcca
    16441 agtttgaact aggacagtct atgctgtctg cagacaagct gatcctagcc atccaaggga
    16501 tcaatatgaa ttttgatgag tttgggcaca aactcaacaa ctatcaagaa tctccacata
    16561 tgcgaattgg tagaagggtt gtggattgct tttcccatca ggatatagct gctttagagc
    16621 agctattgga ggaagtcgat caagaacaga tggcgcagac ctatcgtcgt ttgaatgcca
    16681 ttgtgattaa aaatgcactc cattccttag ataaaagcta cccgctaaca gaggaggata
    16741 gcgagttttt gactacctac ctctacgcta ttgagtcttg gacctggttt gaactctatc
    16801 ttttttgcaa tactatgccc ttcttgagca atcaagatct gatcttttta tcaacctcct
    16861 tactcgaaaa atccaaggaa tttaaagagt tagtacacaa tcgatggtat atgaaaagtg
    16921 gttatcttaa tatcatctca gaacttatgg agcgcaagct attttcttat attccaatct
    16981 ttgaagctga gctggagcgc atgctccgtc catacgatgt ttttgaaaaa gtattgtggc
    17041 agtttttaaa gaaaatgcag atttttcttc aaaccaaagg aagcaatcaa aaagagattg
    17101 aacactttat ccaatctctg caagtattag aaaatccaca attgacagcc ctttttgaat
    17161 tgcgtttgca gcaatacaaa gagcttatcg attagttaga aatgggaaaa agataataat
    17221 atcagcaacg atcgtcaata atgttaaaac ttgtttcttc atattacttc cctcctcatt
    17281 agtaataact ttattatacc agggaaataa tcaaatctac caaaatcgca aatctgaaat
    17341 ttctataaga aaaaatatca aatatgcgat tttctaaaat aagccaattt ccgtgttata
    17401 ctgtccttgt aaaacatttg aagcaaatcc taggtcgcag aagtggttta caaatgaaga
    17461 taattgaagg agtgtaaaag atgttaaatt tacaatttgc agaaacaatg gaattgacag
    17521 aagctgagtt gcaagatgtt agaggaggca acctcgtaaa ttctatggga ggtggtggaa
    17581 gaagtggtat atctggttgg ggcgtcccag gaatttaccc tggttggggc aatcaaggga
    17641 tgagtcctaa tcgtggagct tttgattgga ctatagattt agcagatgga ttgtttggcc
    17701 gccgtagaag ataggctctt ataggagata ataggatgaa gaaaatgaaa gaagtgaaat
    17761 ttcacctagc aactggcttg ttgattttga cttattacct catttttaat gtcacatcag
    17821 acttagattt tatggttgct ttgtctgata atatgtacta tgtgttccaa gttttattgg
    17881 tacttattct gggaactata gctacaattg cttttgtcaa aagtgaacat tggaaagagt
    17941 gtggtcgttt ccagtttcgt tggtcttacc tgggtgtttt ccttctttct tttttcttat
    18001 tgttcgtgtg ggcaaatcta acaacctata tctttcctcg tacccagaat ggaagtacag
    18061 tcgtagaagt agccaccagt ttaacaggaa ttagctattt tgttacgcga atactttata
    18121 ctagtattat cgcacctgta tcagaagagg ttgtttgtcg tggtcttttg atgacaagtt
    18181 tatctaaagt taagagatac tatcttgatg ttcttgtttc cgctgccata tttggcgcta
    18241 tgcatgtttt gcagtatggt tggataacga cagatttcat aaagtatttt ggaatgggct
    18301 tgatattttg tatgatgttt cgctacaccc gatccatcta ttgggctata gctctgcatg
    18361 cttcatggaa tagcttctta ctcattgtta ctttattggt atttggatat tagaaaggtg
    18421 atatatgaaa aagataatct cacaccgcta ctttattata gtttttctac tagttattgc
    18481 tgaccagaag ttcagtgttt tagttttacg cagcgacctt gttgctgggc tatctgactt
    18541 tgcctattat ctgtcggata tgatgttgaa ttttcttgtg gttttatttg ctcttattgc
    18601 tatgatttgg tcggggaaat ggcagaaaat caatagtaga aagtttaaag gatcctatct
    18661 tttctattcc ttcctagctc tgcttgcttt tgttgcttgg aattttgtca ccttttttct
    18721 tttcccacct actcgaaatg aaatttctta tcaacatgct gctcctactt ttacaggagc
    18781 tacggcattt ttgatgtatt ttttttatcc tgtgattgca ggtcccattt ttgaagacat
    18841 gatttatcgt ggattggtga tgacagctct ggaaaaagga aagaaatggg ggctagatgt
    18901 acttggttct gctattttat ttggggtctc gcacattagt aatcacggtt gggtcttgac
    18961 agactttttc gtctatatgg gtggcggact tatatttgca gtcttattta gaatgacaaa
    19021 gtccatttat tggcctattg ggctgcatat agtctacaac ggcattggtc agcttttgat
    19081 gttactgtaa ttttggttgt tagtagtatc gcggtctatt tctggggaaa gattcccatt
    19141 tgaacttaag aaggagttgt gatgaaagta tttcttcaaa atagagattt taggcaatta
    19201 actatcaacc agtggatttc aacgcttggg gatacgattt tttatctggc cttcctgaat
    19261 tatgtggcag atgcgtcttt tgcccctttg gcgattttac ttatcacgat ttcagaaacc
    19321 cttccccaag ttctacaaat ctttctggga gttttggcgg attttcaaca tcatcgtgtc
    19381 ttgaaataca cagtcattag ttttacaaaa tttttgcttt actctatagt gtccctatcg
    19441 ctttcagggc agtctttttc cttgttgcta gtaaccttta tttgtctgct taacctcttg
    19501 tctgacacat tgagttattt ttcaggcgcc atgctcactc cgattttcat tagaattatt
    19561 gggcaagacc atctggcaga agctattgga tttaaacagt caactgttag tttagtgaaa
    19621 acaatcagta atatcctagg aggagtctta ctaggcattc tatccatcca gtttatttcc
    19681 ttactgaacg ctcttacctt tttaatcgca tttttaggta tcctcttcat aaaaactgac
    19741 ctcttgaaag tagaaaaaac gattagctat caagaaggac tctctgtaaa atccttttgc
    19801 cagcatttgc tccaatcatc gaaattgata tggaatatga ataaggtgct cttggttttg
    19861 tttattatct ctactagtca agcagtgata aatgttacgg ttcctatttc cactctattt
    19921 ttaaggaacc agcccttttt gaatttacaa actggtcaat ctcttgcctt gttagccaca
    19981 ctagaattat cagcccttat tttgggaagt ctgatgagtg gctatctgca acataccatt
    20041 tccataaaaa tagccctgta tgcttcactc ctactccaat tactactcct agtgggcttt
    20101 gcgacagctc attttggctt gattctgatc tttagctttt tggatgcctt tttcgcgggt
    20161 attctctctc ctagattgca agaactcgtc tttaaacaaa tacctgagga gtcaatggga
    20221 gcagttcaat cttctatcgg tgccattacg gttgttttac ctagcttatt tacaatagct
    20281 ttgggaacca ttgctactag ctttggaact ctggcagtta gctttgtttt attactattt
    20341 cttctagttg cctttgtcat gcttctgaat attcgtgaaa gtatttagtg gagaatttct
    20401 ataatatatg tttgagactg gattgccagt ctttttatga tggtcaaaat caacagactt
    20461 tttctctttt ctgaatgtgt tatagtagac tgaatctaaa atagtacgaa ataattgcta
    20521 aaacatttat agaa
//
LOCUS       DAVBCW010000024        20487 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVBCW010000024 DAVBCW010000000
VERSION     DAVBCW010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20487
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.024"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(88..2631)
                     /locus_tag="VQ514_000788"
     CDS             complement(88..2631)
                     /locus_tag="VQ514_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HET2499571.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLFPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVAGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQKKVD
                     ILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTKTFT
                     EAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQAQT
                     GFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(2693..4315)
                     /locus_tag="VQ514_000789"
     CDS             complement(2693..4315)
                     /locus_tag="VQ514_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2499572.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
     gene            4521..5546
                     /gene="trpS"
                     /locus_tag="VQ514_000790"
     CDS             4521..5546
                     /gene="trpS"
                     /locus_tag="VQ514_000790"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HET2499573.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            5699..7177
                     /gene="guaB"
                     /locus_tag="VQ514_000791"
     CDS             5699..7177
                     /gene="guaB"
                     /locus_tag="VQ514_000791"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HET2499574.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(7228..8325)
                     /gene="recF"
                     /locus_tag="VQ514_000792"
     CDS             complement(7228..8325)
                     /gene="recF"
                     /locus_tag="VQ514_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HET2499575.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNGN"
     gene            complement(8328..8696)
                     /locus_tag="VQ514_000793"
     CDS             complement(8328..8696)
                     /locus_tag="VQ514_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HET2499576.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SCRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            8855..10105
                     /locus_tag="VQ514_000794"
     CDS             8855..10105
                     /locus_tag="VQ514_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET2499577.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPAVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYRSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKGWIAKLEQV
                     DKDAICRAANNVKLQAIYFMEGIE"
     gene            10102..11385
                     /locus_tag="VQ514_000795"
     CDS             10102..11385
                     /locus_tag="VQ514_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET2499578.1"
                     /translation="MTKVVFEKKYYPAVKEMIYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTFVTEVDGDVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLDLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEITSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            11427..12248
                     /locus_tag="VQ514_000796"
     CDS             11427..12248
                     /locus_tag="VQ514_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2499579.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            12259..12804
                     /gene="pgsA"
                     /locus_tag="VQ514_000797"
     CDS             12259..12804
                     /gene="pgsA"
                     /locus_tag="VQ514_000797"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HET2499580.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIILIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            12801..13628
                     /locus_tag="VQ514_000798"
     CDS             12801..13628
                     /locus_tag="VQ514_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HET2499581.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            13613..14452
                     /locus_tag="VQ514_000799"
     CDS             13613..14452
                     /locus_tag="VQ514_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HET2499582.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            14445..15239
                     /locus_tag="VQ514_000800"
     CDS             14445..15239
                     /locus_tag="VQ514_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HET2499583.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            15298..16116
                     /gene="mreC"
                     /locus_tag="VQ514_000801"
     CDS             15298..16116
                     /gene="mreC"
                     /locus_tag="VQ514_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HET2499584.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTVVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            16116..16610
                     /gene="mreD"
                     /locus_tag="VQ514_000802"
     CDS             16116..16610
                     /gene="mreD"
                     /locus_tag="VQ514_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HET2499585.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            16704..17882
                     /locus_tag="VQ514_000803"
     CDS             16704..17882
                     /locus_tag="VQ514_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HET2499586.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            18106..18885
                     /gene="rpsB"
                     /locus_tag="VQ514_000804"
     CDS             18106..18885
                     /gene="rpsB"
                     /locus_tag="VQ514_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HET2499587.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            18963..20003
                     /locus_tag="VQ514_000805"
     CDS             18963..20003
                     /locus_tag="VQ514_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HET2499588.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            20249..20487
                     /gene="tnpB"
                     /locus_tag="VQ514_000806"
                     /pseudo
     CDS             20249..20487
                     /gene="tnpB"
                     /locus_tag="VQ514_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT         6508 a         3276 c         4244 g         6459 t
ORIGIN      
        1 acttagccct gtgcaataca gaactaaatc cttcggataa attaattgtc taactttttg
       61 gggtcagtac attaactggt cgatttttta tgccttagat gactttcgtc tgtgattata
      121 gattccaaat agtaagagag aagtaaagga acagattgct ccagtaataa aaccattggg
      181 aatgaaggaa agtgtaatag ttcctttccc cttgggaatg tcaactttca taaatccagt
      241 ttgagcttgt ttaatttcta ttttcttacc atcttggtag gcagaccaac ctttgtcata
      301 aggaatggtg aagaaaatag atgtatcttg ttggacatca tatgtagcaa aaaccttgtt
      361 tttagaagtt gatactgtga caggttgttc tttaattttt tgaattgcct cggtgaaagt
      421 tttggtatct aaacgataga aggtaggaga ttcaaatgat acttgtgaat ttccagggaa
      481 actaacattg atattgaaag tttttttctc tttagtatat cctagattaa agaaggagaa
      541 gacattatca gttgtaaaag tctttttttc accatttaca aggatgtcaa ccttcttttg
      601 tttatcgtta gaaaagtgaa ggtttatgaa agagagataa acttggctgt tttctggaac
      661 ttcaatttga tactggattg ctgcatcttc atttgaagaa cttgtgacac taatcaaatc
      721 attagtattt tctgtttttt cataaggtat tggagaaaaa taatcaaaat tgacgttagc
      781 aagttgattt aaaaatgagg cctgattatc caaggtatgt tcattgaact tgacatcatt
      841 gtaaacagat tgactagcaa ctgcaatcgg aagagagtat tgattttcat atagggtaag
      901 attatctttt tgatagatat ctttaaagcc atacttatca ataggactgt ctgagatatt
      961 gtactggata ccaaataaac tatcagccaa aatactatta tttgcatatc ggagattgag
     1021 attagtccca gaggatttaa aaccaagttt atctaaagta gagcttgatg aacgatttcg
     1081 aacagatgaa aattgagaga ttccattgta gttgaatttc atactgtcat ttcctgtctg
     1141 agtttgtagt ttttcagtac gagtaaattg atttccaata tatgttgaga aagattccat
     1201 agctgggata tctcgactat aagcacttcg agaagcaaat ccccattcct tagcaattcc
     1261 gtccatttga gatgaagcat ttaaactcat ttcaaccagt ataaataaag agattagaat
     1321 ggcaaataga ttcacagata taaacttttt gataactgca aggagtaaaa gagaatagac
     1381 aaccaaaaat tcaagagtaa gcagaatatt caaatctgtt aaaaaagaat aatgcgattt
     1441 tagatagatg gtagctaaaa atcctgctac tacaagaaaa agcgaaacta aaaaattcca
     1501 gactttaagt tctttcagac gctttaagac ttctgctgct gtgtaaatta acaaggtaga
     1561 gaaaatccaa gcatagcgat gtaaaaacat gtttggagta tgcatgcctt gccaaaataa
     1621 gtcaagagct tctatgtaaa agcttgcaat tagaaatgca aagaatatta catatatgag
     1681 tttcacgtga aacttaatag atttcagcgt aaaaaataaa atggtcaaaa taaagggaaa
     1741 tagtccaaca aaaatcattg ggatggcccc atactttgtt gtgtcaaagg aaccaatgaa
     1801 ttgcttagca aagagatcaa gataccagct actttcagtt tgaaactttg taacttcagt
     1861 caatttttcc ccatgtgtct gtaaatcaaa tagagtggga agagtcataa tcaaactagc
     1921 cataccagct aaaaaggaga taactatgaa atcaagaaca gatgattttc gagtcttaaa
     1981 gtcccacgaa atttgacaga gataccagaa aataagaaac aatactgtca tatatccaaa
     2041 ataataattt tgaataaata agattgacag acttgtaaag tacaatagga gtttcttttc
     2101 agttatcagt agatgtaaac cagttataat taaaggaatc aagataaaaa catctagcca
     2161 ggtttttatc tctaattgac tgacagtgaa actcatcaga gcataggaag tagataaggc
     2221 tagttttaaa gtctgaggga tagattgaaa caatttattc aaactaaaaa aggttgacag
     2281 accaatcaat ccaaatttta agagagtggt cagatagaca gcatctggca tattcgttag
     2341 atcaaaaaag taaactagag gcgcgagaaa actacccaag taataactag atagggcata
     2401 gaagtttagc cctagaccac ttgtaaaggt gtaaaacaga ctactatttc catgtaggat
     2461 atttcgtaag gctacatcaa aaataacgta ttgatgaaag ccatctccta atagaggaga
     2521 gttgtcgcta ttccagtaga taccttgaga tagatatact ccagacataa tcactacagg
     2581 aatgatgaaa gaaataaaat aggttcgata tgtttttaaa aatgatttca tgttacctcg
     2641 tagaatgata gaaaactcag ttggttaacc caactgagtt ttgaagtttt atttagtctt
     2701 tccaaagttc tttaactttt gcttgtactt ctgcattttc taggaattca tcgtaggttt
     2761 catcgatacg gtcaatgacg ccatttttag ataagacaat gatatggtta gccaaagttt
     2821 gaataaactc gtggtcatgg ctggcaaaga tgattgattc tttaaagttt ttcagtccat
     2881 cattcaagct tgagatagat tccaagtcca agtgatttgt tggatcatca agtacaagga
     2941 catttgattt taagagcatg agttttgaaa gcatgacacg aactttttct ccccctgaca
     3001 agacatttac aggtttgtta acttcatctc cagagaagag catacggccg aggaaaccac
     3061 gtaggaaagt attgtcatct tcttctttac ttgcgaattg acgcaaccag tcaagaattg
     3121 attctcctcc tgcaaaatca gctgagttat cttttggtag gtaagattga ctagttgtaa
     3181 ctccccactt gacagttcct tcatagtcaa tatctcccat gattgcacga attaatgcag
     3241 tcgtttgaat atcattttgt ccaataagtg ctgtcttatc atctggacgc aagatgaaac
     3301 taatattatc caagatagtt tcaccatcaa tctttacagt tagattttct actgtcaaga
     3361 gatcattacc aatctcacgt tccgctttaa agttgataaa tggatattta cgactagatg
     3421 gtacaatctc ttctagctca atcttatcaa gcattttctt acgtgatgtt gcctgccttg
     3481 acttagaagc attggcagag aaacgagcaa cgaattcttg caattgttta attttttctt
     3541 ctgctttagc attacggtct gctagcaatt tagcagcaag ctcagaagat tccttccaga
     3601 agtcgtagtt tccgacatag agtttgattt ttccaaagtc aaggtcggcc atgtgagtac
     3661 aaactttgtt taagaagtga cggtcgtggg atactacgat aactgtgtta tcaaagtcaa
     3721 tcaagaagtc ttctaaccaa gtaatcgatt ggatatccaa accgttagta ggctcgtcca
     3781 agagaagaac atctggttta ccaaaaagtg ctttggcgag gagaaccttt actttttcac
     3841 cgttggccaa ttcgctcatg ttttggtagt gtaattcttc tggaatgttt aggttttgaa
     3901 gtagttgaga ggcttcactc tctgcttccc aacctccaag ttcggcaaac tctccttcga
     3961 gttcggcagc acgaaccccg tcctcgtctg agaaatcttc cttcatgtag atagcatctt
     4021 tctctttcat gatgctataa agtttttcat ttcccatgat aacgacatca atggcacgtt
     4081 catcttcgta gtcaaagtga ttttgacgaa gaacagagag acgttcatct ggaccaagag
     4141 agatgtgacc agtagtaggt tcgatatctc cagctaaaat ttttaaaaag gttgattttc
     4201 cggcaccatt agcaccgatt aatccgtaag tatttccttc tgtaaatttg atattgacat
     4261 catcaaaaag tttgcgatca ctaaaacgta gtgaaacatc agatactgta agcaatgttt
     4321 ttctcctata tgtgtaatat atttattcta ctagaaaata cagaaatatt caaattttta
     4381 tttgtcaatt ttgtgtaaat tatatttaca gtatccttta cacaaatctg taaaaagcaa
     4441 ggctgattta ttttgataaa ttacggttat ttcattaaaa aaatgctata attgaaagga
     4501 ctatatcgaa ggagaacaaa atgactaaac ccattatttt aacaggagac cgtccaacag
     4561 gaaaattgca tattggacat tatgttggaa gtctcaaaaa tcgagtatta ttacaggaag
     4621 aggataagta tgatatgttt gtgttcttgg ctgaccaaca agccttgaca gatcatgcca
     4681 aagatcctca aaccattgta gagtctatcg gaaatgtggc tttggattat cttgcagttg
     4741 gattggatcc aaataagtca actattttta ttcaaagcca gattccagag ttggctgagt
     4801 tgtctatgta ttatatgaat ctagtttcgt tagcacgttt ggagcgaaat ccaacagtca
     4861 agacagagat ttctcagaaa ggatttggag aaagcattcc gacaggattc ttggtctatc
     4921 caatcgctca agcagctgat atcacagctt tcaaggctaa ttatgttcct gttgggacag
     4981 atcagaaacc aatgattgag caaactcgtg aaattgttcg ttcttttaac aatgcatata
     5041 actgtgatgt cttggtagag ccggaaggta tttatccaga aaatgagaga gcagggcgtt
     5101 tgcctggttt agatggaaat gctaaaatgt ctaaatcact aaataatggt atttatttag
     5161 ctgatgatgc ggatactttg cgtaaaaaag taatgagtat gtatacagat ccagatcata
     5221 tccgcgttga ggatccaggt aagattgagg gaaatatggt tttccattat ctagatgttt
     5281 ttggtcgtcc agaagatgct caagaaattg ctgatatgaa agaacgttat caacgaggtg
     5341 gtcttggtga tgtgaagacc aagcgttatc tacttgaaat attagaacgt gaactgggtc
     5401 ctattcgtga gcgccgtatt gaatttgcta aggatatggg agaagtttat aatatgattc
     5461 aaaaaggtag tgaaagagcg cgtgaagttg cgggtcaaac cctatctgag gtaaaagggg
     5521 caatgggact tcattacttt aactaagttt attttacaag aaactatcat ttctatctcg
     5581 aagaaaagat agttttttta tttacccctg taacatttga caaattttta tagaatggta
     5641 tgatagatac aatataaaaa gagtcaagct caaaaagaaa gaaaagagga aacttcgaat
     5701 gtctaattgg gacactaaat ttttgaaaaa aggttttacc tttgatgatg tattgcttat
     5761 tccagctgaa agtcatgtgt tgcctaacga tgcagattta acaactaaat tggcagataa
     5821 tttgacttta aatatcccaa ttattaccgc tgccatggac acagttacag agagtcaaat
     5881 ggccattgct attgctcgtg caggcggtct cggagttatc cataaaaaca tgtcaattgc
     5941 tcaacaagca gacgaggttc gtaaggtaaa acgttctgaa aatggagtta ttattgatcc
     6001 gttcttcttg acgcctgaac atacaattgc tgaagcagat gagcttatgg gtcgttaccg
     6061 catcagtggt gttccagttg ttgaaacact tgaaaatcgt aaattggttg gtattttgac
     6121 aaaccgagat cttcgtttta tttcagatta taatcaacca atttcaaacc atatgactag
     6181 tgaaaatctt gttactgctc ctgtgggtac ggatcttgca acggctgaga gtattcttca
     6241 agagcatcgt attgaaaaac ttccgttggt cgatgaagaa ggcagtcttt ctggtttgat
     6301 cactatcaaa gatattgaaa aagttattga gtttccaaat gcggctaaag atgagtttgg
     6361 tcgtcttcta gttgcaggtg cagtaggtgt tacttcagat acatttgaac gtgcagaggc
     6421 tctttttgag gcaggagcgg atgcgattgt tattgatact gcacatggtc attctgcagg
     6481 tgtcttgcgt aaaattgccg agattcgtgc tcatttccca gatcggactt tgattgctgg
     6541 aaatattgct actgctgaag gtgcacgtgc cctttatgaa gcgggtgtag acgttgttaa
     6601 ggttggtatt ggaccaggtt ctatctgtac tactcgtgtg attgctggtg ttggtgttcc
     6661 gcaagtaaca gctatctacg atgctgcagc tgttgcgcgc gaatatggta aaacgattat
     6721 tgctgacggt gggatcaagt attctggaga tattgtaaaa gcacttgctg caggtggaaa
     6781 tgctgttatg cttggatcta tgtttgctgg aactgatgaa gctccaggcg aaactgaaat
     6841 cttccaagga cgtaaattca agacttaccg tggtatggga tcaattgctg ctatgaagaa
     6901 aggttcaagc gaccgttatt tccaaggttc tgtcaatgaa gcaaacaagc ttgttccaga
     6961 aggaattgaa ggtcgtgttg cttataaagg agcggcagct gatattgttt tccaaatgat
     7021 tggtggtatt cgctctggta tgggttactg tggtgcagct aaccttaaag aactacacga
     7081 taatgctcaa tttattgaaa tgtctggtgc tggtttgaaa gaaagccatc ctcatgatgt
     7141 gcaaattact aatgaggcac caaattattc tatgtaaaaa acaatgaaaa gaactccagt
     7201 gaaaacagga gttcttttac aatgttgtca atttccattt acagcagctt taccatcctg
     7261 aatagtgaag atacttagat tttctggcag attttgaaga tggtctaagc ttgttgttgt
     7321 gataaaggtt tggattgatt gagaaatcgt ttctaataat tttaactgtc tagtgttgtc
     7381 aagttcactc atcacatcgt caagcagtaa tataggagat tctgtggtaa tgctttccat
     7441 taattcgatt tctgctaatt ttatcgagag gacgagacta cgatgttgac cttggcttcc
     7501 gaaactagca tccatcccat ttatataaaa agaaatgtca tctcgatgag gaccgacacc
     7561 agtattcttt ttaaataaat ctctggatct acttttttct aaagcaattt tgaaagattc
     7621 ggataagttt tgtttgtcag ttatattgac agaagattga taggatattg acaactcttc
     7681 gatctgatta gagagttcaa aatgtttctt acgcccaaat gattctagtt tttttatgaa
     7741 atctaagcgg tgattcatta cacgacatcc ataatcaact agctgatcat ctaacacaga
     7801 aaggaatgtt tcatctattt tttgagctga ttttaggtaa gtgtttcttt gctttaggat
     7861 gtggttataa ttggttaagt cagataaata gattggctta atttgcccaa gttccatatc
     7921 aatgaatttt cgtcgaatcg aaggtgctcc tttaattagt tgtaaatctt caggagcaaa
     7981 taagacaaca ttcatgtgtc ctacataatc tgaaaggcgt gcctgtttta agtgattaac
     8041 ttttgttaca cggccttttt gtgttagttc gatttctagg ggaatggatc cagttttttt
     8101 ctgaacgaga cctgaaagat gaagttgttc ctcatcaaaa tgaatgagat ttttatctgt
     8161 tcgagttcga tgactacgcg ttaaggctaa aaaatagata gcctctaaca tgtttgtttt
     8221 tccttgtgca ttacgtccca aaaagacatt caatttagga ttaaagtcta ttttcgtttc
     8281 tttgtaatta cgaaaagtct tgagagatag atgttgtagc cacatgatta tctaccaggg
     8341 aatcgtggag cttgttggct tttaggtgat gaagcaggtt tcgatttgtc ttttttaact
     8401 cccttattca tctttttgac aagtttagcg atccgttctt tttcaacttt atcagcttgg
     8461 tattcatctt gttcttcaga agtaggttgt gtcaacaaga tgtcaatgtt catgtcaggg
     8521 atgtcaactt tatcgccaat acgaagtttt tttccacgac aactctctaa ttccctatta
     8581 aagtaaacag aatgttcaga gagaaatgat ttaatagctc ctccgctatg tgtaattcca
     8641 agttctttga gtagtgcttg gagggtaata aattcttcaa ataatttgta ttccataatt
     8701 cacctcaatc tttggtatta taccatattt tcctaaaaat agtgagagag acagctagaa
     8761 atgtaagcga tttttatttc aaaagtgtta cagggaacaa gaaaatttca ggttttcgtg
     8821 atataataga agtctgtata taaggaggta aatcatggag ttagtgcatg gaatttcaac
     8881 acattttatc caatcaaaaa agtttaaaac aaacaaaatt accgtgcgtt ttaccgctcc
     8941 attatccctt gatacgattg caggtcacat gttgagtgca agtatgctag agactgctaa
     9001 tcagatgtac cccacttctc aagatttgag gagacacttg gccagtctat acggtacaga
     9061 tatgtcaacc aattgtttca gaagagggca aagccacatt atagaattga catttaccta
     9121 tgttcgtgat gagtttttaa gtaggaaaaa cgtgctaacc tctcagattt tggaacttgt
     9181 aaaagaaact cttttttcac ccgcagtagt tgataatggg tttgatccgg ccttatttga
     9241 aattgagaaa aaacaattgc tagcaagttt agcagctgat atggatgatt ctttttattt
     9301 tgcacataaa gaattggata aattgttttt tcatgatgaa cgtcttcaat tggaatatag
     9361 tgatttacga aatcgtattt tagctgaaac tccacaaagt tcttattctt gtttccaaga
     9421 atttttagcc aatgatcgaa tagatttctt tttcctaggt gattttaatg aggttgaaat
     9481 tcaaaatgta ttagaatcat ttggctttaa aggtcgaaaa ggagatgtga aggttcagta
     9541 ttgtcaacct tattctaata tccttcagga aggtatggtt cggaaaaatg tgggacaatc
     9601 cattttggaa ttaggttatc attaccgttc taaatatggt gatgaacaac atttacccat
     9661 gattgtaatg aatggtttac ttggtggatt tgctcactct aagctcttta caaatgtccg
     9721 tgaaaatgct ggattagctt ataccatttc aagtgagctt gatttattta gtggattctt
     9781 gaggatgtat gctggtatca atcgagaaaa tcgtaaccag gctcgtaaaa tgatgaataa
     9841 tcaactgctt gatttaaaaa aaggttattt tacagagttt gagttaaatc agaccaagga
     9901 aatgattcgt tggtcgttgt tactttctca agataatcaa tcttcattga ttgaacgtgc
     9961 ttatcaaaat gccttatttg gaaaatcttc agcagacttt aaaggttgga ttgcaaaact
    10021 cgagcaagtt gacaaagatg ctatttgtag agcagctaat aatgtgaaac tacaggcgat
    10081 ttactttatg gaaggaatag aatgacaaag gttgtttttg aaaaaaaata ctatccagct
    10141 gtaaaagaaa tgatttatcg aactcgtttg gccaacggat tgacagttgc tcttttgcct
    10201 aaaaaggaat ttaaagaggt ttacgggagt gtcactgtac agtttggttc ggtagatacg
    10261 tttgtcacag aagttgacgg agatgtaaaa caatatcctg gaggaattgc tcattttctt
    10321 gaacataaat tatttgagag agaagattct agtgatttga tgtcggcttt tacgagtcta
    10381 ggtgcagata gtaatgcctt tacaagcttt acaaaaacaa actatctttt ttcagcaacg
    10441 gattattttt tagaaaattt agatttactt gatgaattgg taacatcagc acactttact
    10501 gaagcttcca ttctgacaga gcaggatatt attcagcaag aacgagaaat gtaccaagat
    10561 gatccagatt cgtgtttatt cttttcaact ttagcgaatt tgtatcctgg tacaccttta
    10621 gcaactgata tagttggaag tgaggagtcc atttcccaaa tcaatctaac taatttgcaa
    10681 gaaaatttta caaagtttta caaacctgta aacatgtctc tgtttttagt tggtaatttt
    10741 gatgtggagc gagtacagga ctattttgaa agcaaagaac tgaaagattc agattttcag
    10801 gaagtagcaa gagaaaagtt gtttttacag cctgtaaagc caacagatag tatgagaatg
    10861 gaagtatctt ctcccaaact agcgattgga gttagaggta agcgagaagt ttctgaagcg
    10921 gattgctatc gacatcatat tttattaaaa ttattgtttg caatgatgtt tggttggact
    10981 tcggatcgtt ttcaaaaatg ttatgaatca ggtaaaattg atgcgtcctt atctctggaa
    11041 gttgaaataa caagtcgctt tcattttgtc atgttgacaa tggatacgaa agagccagtt
    11101 gctttgtctc atcaatttag gaaggctatt cgtaatttta caaaggattt agatattaca
    11161 gaggaacatt tagatattat caaaagagag atgtttggcg aatttttcag tagcatgaac
    11221 tctcttgaat ttattgcaac gcaatatgat gcttttgaaa atggtgagac aatttttgat
    11281 ttgccgaaaa ttttacagga aattacttta gaggatgtcc ttgatgctgg acatcattta
    11341 atagatgatg gtgacatagt tgattttaca atatttccat cgtagtaacc tatcataata
    11401 gacactagaa agaagggatg acaagtatga gaaaaaaaac aattggagag gttttacgat
    11461 tagctagaat caatcaggga ttgagtttag atgaattgca gaaaaagaca gaaatccagt
    11521 tagatatgtt ggaagcaatg gaagcagacg atttcgatca acttccaagt cctttttaca
    11581 cgcgttcttt cttgaaaaaa tatgcatggg ctgttgagtt agatgaccaa attgttttgg
    11641 atgcttatga ttctgggagt atgattactt atgaggaagt agatgttgat gaagatgagt
    11701 tgacaggtcg tagacgttca agtaagaaaa agaagaaaaa aacatcattt ttacctttat
    11761 tttattttat cctttttgct ttatcgattt taatttttgt gacttattat gtttggaact
    11821 atattcaaac tcaaccagag gagccttctc tttctaatta cagtgtggtt caatcaacaa
    11881 gttcaactag ctctgttccc cactcctcaa gtagtagttc ttctagtata gaatcagcta
    11941 taagtgtatc aggcgaagga aatcatgtag aaatcgctta taagacaagt aaggaaacag
    12001 ttaaattgca attggcagtt tcagatgtta caagttgggt cagtgtttca gaaagcgaac
    12061 ttgagggcgg tgtaacctta tcgccaaaga agaaaagtgc agaagcaaca gttgcaacta
    12121 aaagtcctgt aacaattacg ttaggtgttg taaaaggtgt tgatttgaca gtagataatc
    12181 agactgttga tttatcgaaa ttaacagctc agactggaca aatcactgta acctttacta
    12241 aaaattaagg aaaaacgaat gaaaaaagaa caaattccca atctcttaac aataggtcga
    12301 attctcttta tacctatttt tatctttatt ttaacgatag gaaattcgat agagagtcat
    12361 atagttgcag ctattatctt tgctgttgcc agtattaccg actatttaga tggatattta
    12421 gctcgtaaat ggaatgtggt cagtaatttt ggtaaatttg cagatcctat ggcggataag
    12481 ttactagtta tgtcggcttt tattatcttg attgagttag gtatggctcc ggcttggatt
    12541 gttgcagtga ttatctgtcg tgagttagct gtgacaggtt taaggctttt attggttgaa
    12601 actggtggaa caattttagc agcagcaatg cctggaaaaa ttaaaacttt tagtcagatg
    12661 tttgccatta ttttcttgct attacattgg actttgcttg gtcaagttct actttatgta
    12721 gccttatttt tcactatcta ctctggctat gactatttca agggtagtgc ctatgtattt
    12781 aaagggacat ttggttcgaa atgaaatcaa taattgatgt aaaaaatctt tcttttcgtt
    12841 ataaagaaaa tcagaactac tacgatgtga aggatattac gtttcacgtg aaacgtggag
    12901 aatggctttc gattgtaggg cataatggta gtggtaaatc aacgacggtt cgattaattg
    12961 atggcttact ggaagcagaa tctggagaga ttgtaattga tggccaacgt ttgactgagg
    13021 aaaatgtttg gaatatacgt cgtcaaatcg gtatggtttt tcaaaatcca gacaatcaat
    13081 ttgttggagc gactgttgaa gatgatgttg cttttggttt ggaaaatcag ggactttctc
    13141 gtcaagaaat gaaaaagaga gtggaagaag ctctggcttt agttggcatg ttggacttta
    13201 aaaagagaga gccagcgcgt ctatcaggtg gccaaaagca acgtgtggcc attgcaggtg
    13261 ttgtagccct aagaccagct attttaatct tagatgaagc aacgagtatg ttggatcctg
    13321 aggggcgtag agaacttatt gggacagtaa aaggaattcg aaaagactat gatatgacgg
    13381 tcatttctat tacccatgat ttggaagaag tcgccatgag tgatcgcgta ttggtcatga
    13441 aaaaagggga aattgaatca actagtagtc caagggagct tttctctcga aatgatttag
    13501 atcaaattgg attagacgat ccttttgcta atcaattaaa aaaatctttg agccagaatg
    13561 gctatgattt acctgaaaat tatttgacag aaagtgagct agaggataag ctatgggaat
    13621 tgctctagaa aatgtgaatt ttacatatca agaaggtact cccttagctt cagcagcttt
    13681 gtcggatgtt tctttgacga ttgaagatgg ctcttataca gctttaattg ggcacacagg
    13741 tagtggtaaa tcaactattt tacaactctt aaatggttta ttggtgccaa gtcaagggag
    13801 tgtgagggtt tttgatacct taatcacctc gacttctaaa aataaagata ttcgtcaaat
    13861 tagaaaacag gttggcttgg tatttcagtt tgctgaaaat cagatttttg aagaaacggt
    13921 tttgaaggac gttgcttttg gaccgcaaaa ttttggagtt tctgaagaag atgctgtgaa
    13981 gactgcgcgt gagaaactgg ctctggttgg aattgatgaa tcactttttg atcgtagtcc
    14041 gtttgagctg tcagggggac aaatgagacg tgttgccatt gcaggcatac ttgccatgga
    14101 gccagctata ttagtcttag atgagccaac agctggtcta gatcctctag ggagaaaaga
    14161 gttgatgacc ctgttcaaaa aactccacca gtcagggatg accatcgtct tggtaacgca
    14221 tttgatggat gatgttgctg aatatgcaaa tcaagtctat gtaatggaaa agggacgttt
    14281 agtaaagggg ggcaaaccaa gtgatgtctt tcaagacgtt gtttttatgg aagaagttca
    14341 gttgggagta cctaaaatta cggccttttg taaacgattg gctgatagag gcgtgtcatt
    14401 taaacgatta ccgattaaga tagaggagtt caaggagtcg ctaaatggat agtatgattt
    14461 tggggcgtta tatcccaggg gattcgattg ttcaccgatt ggatccacgt agcaaattgc
    14521 ttgctatgat gctactgatt ttgattgtgt tttgggctaa taaccccttg acgaatctga
    14581 ttctttttat agcgacaggg atatttattg ccttgtcagg agtatctctt tcatttttta
    14641 ttcagggctt gaagtctatg tttttcttga ttgccttcac aactattttt caactatttt
    14701 tcatttctaa tgggaatgtt ttatttgagt tttcgtttgt gagaatcacg gattatgctt
    14761 tgcaacaagc tgggattatt ttttgtcgct ttgtattgat tattttcttt tcaactttgt
    14821 taaccttaac aaccatgcct ttaagtttgg catcagctgt cgaagcttta ttagcacctt
    14881 taaagcgtgt gaaagttcca gttcatgaaa ttggattgat gctgtctatg agtttgcgtt
    14941 ttgtcccaac cttgatggat gatacgactc ggattatgaa tgcacagaaa gctcgtggag
    15001 tggattttgg agaaggaagc atcgttcaaa aagtaaaggc gatgattccc attttgattc
    15061 ctctttttgc gacaagttta aaacgtgcag attccttggc tatcgctatg gaagcgcgtg
    15121 gctatcaggg tggaaaaggc agaagtcaat accgacaatt gaaatggact ctaaaggata
    15181 cgctgaccat tcttgttatt ctcgtacttg gttgttgttt atttttctta aaatcttagt
    15241 agctttcagg aattgataaa aagttactgt aacagttttt gatataaagg taggaatatg
    15301 aaccgtttta aaaaatcaaa atatgtcatt attgtttttg tcactgttct gcttgtgtca
    15361 gctctcttag cgacgactta ttcaagtaca gttgtgacaa aattaggaga tggaatctca
    15421 ttggttgata gagttgtaca aaaacctttt cagtggtttg attctgtcaa atcagatttg
    15481 gctcatttga cacgaacata taatgaaaat gaaagtttga agaaacagct ttaccaatta
    15541 gaagttaaat caaatgaggt ggaaagttta aagacagaaa atgaacaact gcgccaattg
    15601 cttgatatga agtctaaatt gcaagccaca aagactttag cagcagatgt tattatgcgt
    15661 tctccggtat cttggaagca ggagttgacc ttagatgcag gtagatcaaa aggtgcttct
    15721 gagaacatgt tagctattgc aaatggtggc ttgattggga gtgtttcaaa agtagaggag
    15781 aactctacta tagtcaacct tctgacaaat acggaaaatg ctgataagat ttctgttaaa
    15841 attcaacatg gctctactac aatttatgga attattattg gctatgacaa ggaaaatgac
    15901 gttcttaaaa ttagccaatt aaatagtaat agcgatatta gtgcgggaga taaggtgact
    15961 actggtggat taggaaactt taacgttgct gatattcctg ttggtgaagt ggttgccaca
    16021 acgcatagta cagactattt gacacgagaa gtaactgtta aattgagtgc agatactcat
    16081 aatgtagatg tgatagaatt agtggggaat tcataatgag acagttgaag cgagttggag
    16141 tatttttatt gcttcctttc tttgttctaa ttgacgccca tattagccag cttctgggct
    16201 catttttccc ccatgtacat ttggctagtc attttctttt tctatttctc ttatttgaga
    16261 cgatagaagt atcagagtat ctctacctag tctattgttt tgttataggc ttggtttatg
    16321 atgtttactt tttccatcta atagggatta caactctctt atttatctta ttgggagcct
    16381 tccttcataa attgaatagt gttattttgt tgaatcgttg gacaagaatg ctagctatga
    16441 ttgtgctgac attcctgttt gaaatgggta gttatctttt ggcttttatg gtagggttga
    16501 cagtagatag catgtcgatt tttatagtct atagcttggt accgacgatg attttaaatt
    16561 ttttatggat tactgttttt caatttattt ttgaaaaata ttatctataa gaacgacata
    16621 taaatgtaac aaaggcgtaa tatttattag gccttttttt ggtatactag tattgtcttt
    16681 aaaagaagga gtatctacgt aatatgaaga aaaaaatctt agcgtcactt ttattaagta
    16741 cagtaatggt ttctcaagta gctgttttaa caactgcgca tgcagaaacg actgatgaca
    16801 aaattgctgc tcaagataat aaaattagta acttaacagc acaacaacaa gaagcccaaa
    16861 aacaagttga ccaaattcag gagcaagtat cagctattca agctgagcag tctaacttgc
    16921 aagctgaaaa tgatagatta caagcagaat ctaagaaact cgagggtgag attacagaac
    16981 tttctaaaaa cattgtttct cgtaaccaat cgttggaaaa acaagctcgt agtgctcaaa
    17041 caaatggagc cgtaactagc tatatcaata ccattgtaaa ctcaaaatca attacagaag
    17101 ctatttcacg tgttgctgca atgagtgaaa tcgtatctgc aaacaacaaa atgttagaac
    17161 aacaaaaggc agataaaaaa gctatttctg aaaaacaagt agcaaataat gatgctatca
    17221 atactgtaat tgctaatcaa caaaaattgg ctgatgatgc tcaagcattg actacgaaac
    17281 aggcagaact aaaagctgct gaattaagtc ttgctgctga gaaagcgaca gctgaagggg
    17341 aaaaagcaag tctattagag caaaaagcag cagctgaggc agaggctcgt gcagctgcgg
    17401 tagcagaagc agcttataaa gaaaaacgag ctagccaaca acaatcagta cttgcttcag
    17461 caaacactaa cttaacagct caagtgcaag cagtatctga atctgcagca gcacctgtcc
    17521 gtgcaaaagt tcgtccaaca tatagtacaa acgcttcaag ttatccaatt ggagaatgta
    17581 catggggagt aaaaacattg gcaccttggg ctggagacta ctggggtaat ggagcacagt
    17641 gggctacaag tgcagcagca gcaggtttcc gtacaggttc aacacctcaa gttggagcaa
    17701 ttgcatgttg gaatgatggt ggatatggtc acgtagcggt tgttacagct gttgaatcaa
    17761 caacacgtat ccaagtatca gaatcaaatt atgcaggtaa tcgtacaatt ggaaatcacc
    17821 gtggatggtt caatccaaca acaacttctg aaggttttgt tacatatatt tatgcagatt
    17881 aatttacaga gggactcgaa tagagccctc ttttcaggtt ttaccgtgac aatccctatt
    17941 aaaaattata tcaaaatagc ttgaaaatat tggaaaagta tggtagaatg aaaattgtcg
    18001 tgtgaacgat aatactcatt cttgatgaat tgtgaagcag ttgcccttgg gtcgttttgc
    18061 gagttgaagt caagaagagg aaaaaaacaa aaaggagaaa tactcatggc agtaatttca
    18121 atgaaacaac ttcttgaggc tggtgtacac tttggtcacc aaactcgtcg ctggaatcct
    18181 aagatggcta agtacatctt tactgaacgt aacggaatcc acgttatcga cttgcaacaa
    18241 actgtaaaat acgctgacca agcatacgac ttcatgcgtg atgcagcagc taacgatgca
    18301 gttgtattgt tcgttggtac taagaaacaa gcagctgatg cagttgctga agaagcagta
    18361 cgttcaggtc aatacttcat caaccaccgt tggttgggtg gaactcttac aaactgggga
    18421 acaatccaaa aacgtatcgc tcgtttgaaa gaaattaaac gtatggaaga agatggaact
    18481 ttcgaagttc ttcctaagaa agaagttgca cttcttaaca aacaacgtgc gcgtcttgaa
    18541 aaattcttgg gcggtatcga agatatgcct cgtatcccag atgtgatgta cgtagttgac
    18601 ccacataaag agcaaatcgc tgttaaagaa gctaaaaaat tgggaatccc agttgtagcg
    18661 atggttgaca ccaatactga tccagatgat atcgatgtaa tcatcccagc taacgatgac
    18721 gctatccgtg ctgttaaatt gatcacagct aaattggctg acgctattat cgaaggacgt
    18781 caaggtgagg atgcagtagc agttgaagca gaatttgcag cttcagaaac tcaagcagat
    18841 tcaattgaag aaatcgttga agttgtagaa ggtgacaacg cttaatttat acaaatagta
    18901 attacctagg agggcggggc ttagcccggc tctcctattt tcaaaaatat aggagaatta
    18961 aaatggcaga aattacagct aaacttgtaa aagagttgcg tgaaaaatct ggtgccggtg
    19021 ttatggacgc taaaaaagcg cttgtagaaa cagacggtga catcgaaaaa gcgattgaat
    19081 tgcttcgtga aaaaggtatg gctaaggcag ctaagaaagc tgaccgtgtt gctgcagaag
    19141 gtttgactgg tgtttatgtt aacggtaatg ttgcagcagt tattgaagta aatgctgaaa
    19201 ctgacttcgt tgcaaaaaac gctcaattcg ttgaattggt aaatactaca gctaaagtca
    19261 ttgctgaagg aaaacctgct aacaatgaag aagctcttgc tttgataatg ccttcaggtg
    19321 aaactcttga agctgcatac gtatctgcaa cagcaactat cggagagaaa atctcattcc
    19381 gtcgctttgc attgattgaa aaaacagacg cacaacactt tggagcatac caacataacg
    19441 gtggacgtat cggtgttatt tcagttgttg aaggtggaga cgaagcactt gctaaacaat
    19501 tgtcaatgca catcgcagcg atgaaaccaa cagttctttc ttacaaagaa ttggatgagc
    19561 aattcgttaa agatgagttg gcacaattga atcacgttat cgaccaagac aacgaaagcc
    19621 gtgcaatggt taataaacca gctcttccac acttgaagta tggatcaaaa gctcaattaa
    19681 ctgatgatgt tattgctcaa gctgaagctg acatcaaagc tgaattggct gcagaaggca
    19741 aaccagaaaa aatctgggac aaaattatcc caggtaaaat ggatcgcttc atgcttgata
    19801 atactaaagt tgaccaagct tacacacttc ttgcacaagt ttacatcatg gatgacagca
    19861 agacagttga agcatacctt gaatcagtta acgcttcggt agttgagttt gctcgctttg
    19921 aagttggtga aggtatcgag aaagctgcaa acgacttcga agctgaagtt gcagctacaa
    19981 tggcagcagc cttgaataac taataataga aaggaagcaa attgcttcct tttttgtata
    20041 gggcttagat ttttcctgaa attgtttttt ccccagtctc actcgattag aataattaaa
    20101 acaaaaagcc ctataataag ggctaagtgc atttaggaga ctatacttca aattcatagt
    20161 aagcgcgtca taacaaggta tctatcctaa ttccccaact tccagtatac tagataaaaa
    20221 ataattatcg gaggaaaggt attgtactat gataattcaa ctaagtgatt taggtcaagt
    20281 tcaccttgtt tgtggcaaga cagatatgag gtagggaata gactcattag cctatgtact
    20341 taaaacccac tttgaattgg attctttctc cggtcaagtc tttctctttt gtggtggacg
    20401 taaagaccgc tttaaagtcc tttactggga tggtcaagga ttttggctac tatataaacg
    20461 ctttgagaac ggaaaactga cttggct
//
LOCUS       DAVBCW010000025        19939 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVBCW010000025 DAVBCW010000000
VERSION     DAVBCW010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19939
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.025"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            416..1369
                     /locus_tag="VQ514_000807"
     CDS             416..1369
                     /locus_tag="VQ514_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET2499589.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLISLDKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKEWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKSEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            1420..2610
                     /locus_tag="VQ514_000808"
     CDS             1420..2610
                     /locus_tag="VQ514_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HET2499590.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGTDAIVFTAGVGE
                     NAENFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            2759..3130
                     /gene="rnpA"
                     /locus_tag="VQ514_000809"
     CDS             2759..3130
                     /gene="rnpA"
                     /locus_tag="VQ514_000809"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HET2499591.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            3105..3929
                     /locus_tag="VQ514_000810"
     CDS             3105..3929
                     /locus_tag="VQ514_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET2499592.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAKIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            3948..4934
                     /locus_tag="VQ514_000811"
     CDS             3948..4934
                     /locus_tag="VQ514_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HET2499593.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQAIVDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNRYSKTFYVTINVNDYVEHRAEVLQTYAQKL
                     AIRVLEEGRSHQTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            4985..5608
                     /locus_tag="VQ514_000812"
     CDS             4985..5608
                     /locus_tag="VQ514_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HET2499594.1"
                     /translation="MLNKIRDYLDFAGLQYRNPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPGWQLQQTSQWMNQAQRLRPHFWVYLQRDEQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQAKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            5739..7196
                     /locus_tag="VQ514_000813"
     CDS             5739..7196
                     /locus_tag="VQ514_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HET2499595.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            7219..7500
                     /locus_tag="VQ514_000814"
     CDS             7219..7500
                     /locus_tag="VQ514_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2499596.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            7577..8062
                     /locus_tag="VQ514_000815"
     CDS             7577..8062
                     /locus_tag="VQ514_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2499597.1"
                     /translation="MNLKQALIDNDSIRLGLEANNWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            8079..8744
                     /locus_tag="VQ514_000816"
     CDS             8079..8744
                     /locus_tag="VQ514_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HET2499598.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAADPAGAARAFKDEIKR
                     IWG"
     gene            8748..9611
                     /locus_tag="VQ514_000817"
     CDS             8748..9611
                     /locus_tag="VQ514_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HET2499599.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKEIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIN
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SRGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            9613..10317
                     /locus_tag="VQ514_000818"
     CDS             9613..10317
                     /locus_tag="VQ514_000818"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HET2499600.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVAWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            10496..12169
                     /locus_tag="VQ514_000819"
     CDS             10496..12169
                     /locus_tag="VQ514_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HET2499601.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALGDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            12281..13372
                     /gene="ulaG"
                     /locus_tag="VQ514_000820"
     CDS             12281..13372
                     /gene="ulaG"
                     /locus_tag="VQ514_000820"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HET2499602.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            13486..15462
                     /gene="tkt"
                     /locus_tag="VQ514_000821"
     CDS             13486..15462
                     /gene="tkt"
                     /locus_tag="VQ514_000821"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET2499603.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVTDYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDANRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKVVRNLK"
     gene            15579..15878
                     /gene="yajC"
                     /locus_tag="VQ514_000822"
     CDS             15579..15878
                     /gene="yajC"
                     /locus_tag="VQ514_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET2499604.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVEGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            15925..16353
                     /locus_tag="VQ514_000823"
     CDS             15925..16353
                     /locus_tag="VQ514_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HET2499605.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            16355..16765
                     /locus_tag="VQ514_000824"
     CDS             16355..16765
                     /locus_tag="VQ514_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499606.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            17065..19716
                     /gene="adhE"
                     /locus_tag="VQ514_000825"
                     /gene_synonym="adhC"
     CDS             17065..19716
                     /gene="adhE"
                     /locus_tag="VQ514_000825"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HET2499607.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITEPSMEATSALMNHEGIATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT         6262 a         3573 c         4327 g         5777 t
ORIGIN      
        1 aagtcgtgcg ctgaacacca acaagacgct taaattgtgc attggttagt tgtttacttt
       61 cttcataaat tttattgtat catatttttg tttcacagga agtctaataa ataaaaattc
      121 tttaaaattt tgttgggaaa atgggaaaag atataaatgg aaacttagta taacacttgg
      181 aaaactctgt aaaaatagta agtcaattca tgtcccgttt tttctctagg gaagttgggc
      241 ggctatattt atatgaccat ttgtcgtaat aaacatccct accaagggtc aatagaaata
      301 aattcaacga aacttttgct gtcagattta gatgcccaat ttgtcattta tactctttca
      361 tataggactt tctataagga tggaaaaaag aagggaaatt tgataagata ggaatatgga
      421 ttttgaaaaa attgaacaag cttataccta tttactagag aatgtccaag tcatccaaag
      481 tgatttggcg accaactttt atgacgcctt ggtggagcaa aacagcatct atctggatgg
      541 tgaaactgag ctaaaccagg tcaaagacaa caatcaggcc cttaagcgtt tagcactacg
      601 caaagaagaa tggctcaaga cctaccagtt tctcttgatg aaggctgggc aaacagaacc
      661 cttgcaggcc aatcaccagt ttacaccgga tgctattgct ttgcttttgg tgtttattgt
      721 ggaagagttg tttaaagagg aggaaattac tatcctcgaa atgggttctg ggatgggaat
      781 tctaggcgct actttcttga tctcgcttga taaaaaggtg gattacttgg gaatggaagt
      841 ggatgatttg ctgattgatc tggcagctag catggcagat gtaattggtt tgcaggctgg
      901 ctttgtccaa ggagatgccg ttcgtccaca aatgctcaaa gaaagcgatg tggtcatcag
      961 tgacttgcct gttggctatt atcctgatga tgccgttgcg tcgcgccatc aagttgcttc
     1021 tagccaagaa catacttacg cccatcactt gctcatggaa caagggctta agtacctcaa
     1081 gtcagacgga tacgctattt ttctagctcc gagtgatttg ttgaccagtc ctcaaagtga
     1141 tttgttaaaa gaatggctga aagaagaggc gagtctggtt gctatgatta gtctgcctga
     1201 aaatctcttt gctaatgcta aacaatctaa gactattttt atcctacaga agaaaagtga
     1261 aatagcagtg gagccttttg tttatccact tgctagcttg caagatgcaa gtgttttaat
     1321 gaaatttaaa gaaaattttc aaaaatggac tcaaggtact gaaatataaa atagattttg
     1381 ttataatagt tgaaaacgct taaaaagggg tatcatgtta tgacaaaaac aattgcaatc
     1441 aatgcaggaa gttcaagttt gaaatggcaa ttatacttaa tgccagagga aaaagtgtta
     1501 gccaaaggtt tgattgaacg tatcggtttg aaagattcaa tttcaactgt aaaatttgac
     1561 ggccgttctg aacaacaaat tttggatatc gaaaatcata cacaagctgt taaaatttta
     1621 ttggatgact tgattcgttt cgatattatc aaggcttatg acgagattac aggtgttggc
     1681 caccgtgttg tagcaggtgg agaatatttc aaagaatcaa cagttgttga gggagatgtt
     1741 ttagaaaaag ttgaagagtt gagtttgttg gctcctctac acaacccggc caatgcagca
     1801 ggtgttcgtg ctttcaagga attgttgcca gacattacca gtgtagttgt ttttgatact
     1861 tccttccaca caagtatgcc agagaaagct tatcgctacc ctctaccaac aaaatattac
     1921 acagaaaaca aggttcgtaa atacggtgct catggtacaa gtcaccagtt tgtagcagga
     1981 gaagctgcaa aactcttggg acgtccatta gaagacttga agttaattac ctgtcatatt
     2041 ggtaacggag gctcaattac agctgtgaaa gccggcaaat ctgtagacac ttctatgggg
     2101 ttcactcctc ttggtggtat tatgatggga acgcgtacag gggatattga tccagctatc
     2161 attccttatt taatgcaata tacagaggat tttaacacac cagaagatat cagtcgtgtt
     2221 cttaaccgtg aatcaggtct tttgggagtt tctgctaatt ctagcgatat gcgcgatata
     2281 gaagcagctg tagcagaagg gaatcacgag gctagcttgg cttatgaaat gtatgttgac
     2341 cgtatccaaa aacatatcgg tcagtacctt gcagtgctaa atggaacaga tgccattgtt
     2401 ttcacagcag gtgtcggtga aaatgcagag aatttccgtc gtgatgtaat ctcagggatt
     2461 tcgtggtttg gttgtgatgt tgatgatgaa aagaatgtct ttggcgttac aggagacatc
     2521 tcaacagagg cagctaaaat ccgtgtcttg gttattccaa cagatgaaga attagtcatt
     2581 gcccgtgacg ttgaacgctt gaaaaaataa gtgaaactaa aaaaatattc aatacaagga
     2641 gttgggaaag ttatttttcc agcttctttt tctgatgaaa ttgtccaaaa ccttgctatg
     2701 attggctttt ttgaaaaata tggtataata gtagtaattt aatagatgga gttgagtttt
     2761 gaagaaaaac tttcgtgtaa aaagagagaa agattttaag gcgattttca aggaggggac
     2821 aagttttgct aatcgcaaat ttgtggtcta ccaattagaa aaccagaaaa accattttcg
     2881 agtaggtcta tcagttagca aaaaactggg gaatgccgtc actagaaatc aaattaagcg
     2941 acggattcgg catattatcc agaatgcaaa agggagtctg gtagaagatg tcgactttgt
     3001 tgtcattgct cgaaaaggag tcgaaacctt gggatacgca gagatggaga aaaatctact
     3061 ccatgtatta aaattatcaa agatttaccg ggaaggaaat gggagtgaaa aagaaactaa
     3121 agttgactag tttgctagga ctgtctctgt taatcatgac agcctgtgcg actaatgggg
     3181 taactagcga tattacagcc gaatcggctg atttttggag taaattggtt tacttctttg
     3241 cgaaaatcat tcgcttttta tcgtttgata ttagtatcgg agtggggatt attctcttta
     3301 cggtcttgat tcgtacagtc ctcttgccag tctttcaggt gcaaatggtg gcttctagga
     3361 aaatgcagga agctcagcca cgcattaagg cgcttcgaga acaatatcca ggtcgagata
     3421 tggaaagcag aaccaaacta gagcaggaaa tgcgtaaagt atttaaagaa atgggtgtca
     3481 gacagtcaga ttctctttgg ccgattttga ttcagatgcc ggttattttg gccttgttcc
     3541 aagccctatc aagagttgac tttttaaaga caggtcattt cttatggatt aaccttggta
     3601 gtgtggatac aacccttgtt cttccgattt tagcagcagt attcaccttt ttaagtactt
     3661 ggttgtccaa caaagctttg tctgagcgaa atggcgctac gactgcgatg atgtatggga
     3721 ttccagtctt gatttttatc tttgcagttt atgcgccagg tggagtcgcc ctatactgga
     3781 cagtgtctaa tgcttatcaa gtcttgcaaa cctatttctt gaataatcca ttcaagatta
     3841 tcgcagagcg cgaggccgta gtacaggcac aaaaagattt ggaaaataga aaaagaaaag
     3901 ccaagaaaaa ggctcagaaa acgaaataaa taaggaggaa tctggtaatg gtagtattta
     3961 caggttcaac tgttgaagaa gcaatccaga aaggattgaa agaattagat attccaagaa
     4021 tgaaggctca tatcaaagtc atttctaggg agaaaaaagg ctttcttggt ctatttggta
     4081 aaaaaccagc ccaagtggat attgaagcga ttagtgaaac gactgttgtc aaagcaaatc
     4141 aacaggtagt aaaaggcgtt ccgaaaaaaa tcaatgattt gaacgagcct gtgaagacgg
     4201 ttagtgaaga aaccgttgac cttggtcatg tggttgatgc tattaaaaaa atagaggaag
     4261 aaggtcaagg tatttctgat gaagtcaagg ctgaaatctt aaaacatgaa agacatgcca
     4321 gcactatctt agaagaaact ggtcacattg agattttaaa tgaacttcaa atcgaggaag
     4381 cgatgaggga agaagcaggc gctgatgacc ttgaaactga gcaagatcaa actgaaaatc
     4441 aagacttgaa agagatgggc ttgaaggtcg agcaaagtta tgatattgcc caggtggcta
     4501 cggatgtgac tgcctatgtt caagcgattg tggatgacat ggatgttgaa gctacacttt
     4561 caaatgatta taaccgtcgt agcatcaatc tacaaattga caccaacgaa ccaggtcgta
     4621 ttatcggcta ccatggtaaa gtcttgaagg ccttgcaact gttggctcaa aattatcttt
     4681 acaaccgcta ttccaaaacc ttctacgtta caatcaatgt caatgattat gtcgaacacc
     4741 gtgcagaagt cttgcagacc tatgcgcaaa aattggcgat tcgtgtttta gaagaaggac
     4801 gtagtcatca aacagatcca atgtcgaata gtgaacgcaa gattatccac cgtattattt
     4861 cacgtatgga tggcgtgacc agttactctg aaggtgatga gccaaatcgc tatgtcgttg
     4921 tagatacaga ataagtaaaa tcagggttga cctgattttt tgctagctag aggaggttaa
     4981 actaatgttg aataagataa gagactattt agactttgct ggtttgcagt accgtaatcc
     5041 tgataaagcg ggagcagagc gagagaagat gctggcattc cgccacaaag gacaagaggc
     5101 ccgaaaggct tttacagaac tggccaaagc ctttcaagca agccatccag gatggcaact
     5161 ccaacagact agtcagtgga tgaatcaggc ccagcgtttg agaccacatt tttgggttta
     5221 tctacagaga gacgaacaag tgacagaacc tatgatggcc ttacgtttgt atgggacatc
     5281 tactgacttt ggaatttctt tggaagtcag tttcatcgaa cgtaagaagg atgagcaaac
     5341 actgggcaag caggccaaag ttttagacat tccaaccgtt aaagggattt attatctaac
     5401 ctactctaat ggtcaaagtc aacggtggga ggcgaatgaa gaaaagcgtc gtactttacg
     5461 cgagaaggtg agaagtcaag aagttcgaaa agttttagtg aaggtagatg ttcctatgac
     5521 agaaaattcg tctgaagaag aaatcgtaga aggcttattg aagtcttatt ctaaaattct
     5581 tccctattat ctagctacga gaaaataaga taatttgtaa aacatcataa atcatacagt
     5641 ccaagagtga acagtccgct gtgtaattct tggtcttttt gtttgcgctt tcgcattata
     5701 taataaactt acaaaaacaa ttcaaaagga gaacaattat ggaagtcgtt tcaagtgttc
     5761 taaattggtt ttctagcaat attttgcaga atcccgcatt tttcgtaggt ttattggtgt
     5821 tgataggata tgcacttttg aaaaaacctg cccatgacgt tttttcaggg tttgttaaag
     5881 caacagtagg gtatatgttg cttaacgtgg gtgctggtgg tttggttaca acctttcgtc
     5941 caatcttagc agctcttaac tacaaattcc aaattggtgc agcggttatc gacccttact
     6001 ttggacttgc tgcagcaaac aacaaaattg cagcagagtt tccagatttt gttggaactg
     6061 caactacagc tctattgatt ggttttggaa taaatatctt gctcgtagct cttcgaaaga
     6121 ttacgaaggt aagaaccctc tttattactg gtcacatcat ggtacaacaa gctgcaacag
     6181 tatctcttat ggttctattc ttagtaccac aattgcgcaa tgcttacggt acagcagcga
     6241 ttggtatcat ctgtggactt tactgggcag ttagttcaaa tatgactgtt gaggcaactc
     6301 aacgcttgac tggtggtggc ggatttgcga ttggtcacca acagcaattt gcaatctggt
     6361 ttgtagataa agtagcagga cgctttggta agaaagaaga aagtttagac aatcttaaat
     6421 tacctaagtt cctctcaatc ttccacgata cagttgttgc atctgctacc ttgatgctcg
     6481 tattcttcgg agccattctt ttaatcttgg gtccagacat tatgtctaat aaagaagtca
     6541 tcacttcagg aactctattc aatcctgcta aacaagattt ctttatgtac attatccaaa
     6601 cagcctttac cttctcagtt tacttgttcg ttttgatgca aggtgtccga atgttcgtat
     6661 ctgagttgac aaacgccttc caaggtattt caaacaaatt gttgccaggt tcattcccag
     6721 cggttgacgt tgcagcttct tatggatttg gttctccaaa tgctgtcttg tcaggattta
     6781 cctttggttt gattggtcaa ttgattacaa ttgttttgct catcgtcttt aaaaatccga
     6841 ttcttattat tacaggattt gtaccagtgt tctttgacaa tgcagccatt gcggtctacg
     6901 ctgataaacg cggcggatgg aaagcggctg ttatcctttc ctttatatca ggtgtccttc
     6961 aagttgctct aggagctctt tgtgtggccc ttctcgattt ggcatcttat ggtggctacc
     7021 atggaaatat cgactttgaa ttcccatggc ttggatttgg atatatcttc aaataccttg
     7081 gtattgttgg ttatgtactt gtgtgtctct tcttgcttgt tattcctcaa cttcaatttg
     7141 ccaaagcaaa agataaagag aaatattaca acggtgaagt tcaagaagaa gcttagtatc
     7201 tagaaaagga gaaataaaat ggttaaagta ttagcagcgt gcggaaatgg aatgggttca
     7261 tcaatggtta tcaagatgaa ggttgaaaat gctctccgta agcttaatca aacagatttt
     7321 acagtcaatt catgcagtgt cggtgaagct aaaggtttag cagtaggata tgacattgta
     7381 atcgcttctc ttcatttgat tcaagaattg gaagggcgaa ctaatgggaa gttaattggg
     7441 cttgataact tgatggatga taaagaaatc accgaaaaac tcagtcaagc actacagtaa
     7501 aaggttggag ggggctggac agaaactgag agttatcgtt tctgtccagc cccctcttta
     7561 aataaaggag gcagatatga atttaaaaca agctttaatt gacaatgact cgatccgact
     7621 aggtttagag gctaacaatt ggaaagaagc agtcaaggta gcagtagatc ccttgattga
     7681 aagtggggca attttgccag agtattacga tgctatcatt gaatcgactg aagagtatgg
     7741 gccttactat atcttgatgc caggtatggc tatgccccac gctagacctg aagctggtgt
     7801 gcaaagtgat gccttttcat tgattacctt acaaaatcct gttgtatttt cagatgggaa
     7861 agaggtatct gttttgttgg cactagcagc aacaagttca aaaattcaca caagtgtagc
     7921 cattccacaa attattgccc tgtttgaatt agaagattct attgcacgtt tacaggcttg
     7981 ccagactaaa gaagatgtct tggctatgat tgaagaatct aaggatagcc cttatctcga
     8041 aggattggat ttggaaagtt agaaagagga ataaagaaat gacaaaaaga atacctaatt
     8101 tacaagttgc attagaccat tcagacttgc aaggagcgat taaagcagct gtttctgttg
     8161 gtcaggaagt agatattatc gaagctggaa ctgtttgctt gcttcaagtt ggaagtgaac
     8221 tggctgaagt cttgcgtagc cttttcccag ataagattat tgtggcagac acaaaatgtg
     8281 ctgatgctgg tggaacagtt gctaaaaata atgcggttcg tggagcagac tggatgactt
     8341 gtatctgttg tgcaaccatc cctactatgg aagcagctct aaaggctatc aagactgaac
     8401 gaggagaacg aggcgaaatc cagatcgagc tttatggcga ttggactttt gaacaagctc
     8461 agctttggct agatgcaggt atctcacaag ctatttatca ccaatctcgt gatgctcttc
     8521 ttgctggtga aacttggggt gaaaaagacc ttaataaggt taaaaaactc attgacatgg
     8581 gcttccgtgt atctgtaaca ggtggtctag atgtagatac tctcaaactc tttgaaggtg
     8641 ttgatgtctt tacctttatc gcaggtcgtg gaattacaga ggctgcggat ccagcaggag
     8701 cagcgcgtgc cttcaaggat gaaatcaaac gaatttgggg gtaaatcatg gtacgtccaa
     8761 ttggaattta tgaaaaggca accccaacac actttacttg gctagaacgt ttaaattttg
     8821 ccaaggagtt aggctttgat tttgtcgaga tgtctattga cgaacgtgac gagcgtttag
     8881 caagacttga ctggagtaag gaagaacgct tggaagttgt caaagaaatc tatgaaactg
     8941 gtgttcgtat tccttctatc tgtttttcag gccatcgtcg ctacccattg ggttcaaaag
     9001 atccagttct agaggaaaaa tctctagaac tcatgaaaaa atgtatcgaa ttagctcaag
     9061 acttgggagt tcgtacgatt caattagctg gttacgatgt ttactatgag gaaaagtcac
     9121 cccagacacg ccaacgtttt atcaaaaatt tgagaaaagc ctgtgactgg gctgaagaag
     9181 ctcaggtggt acttgctatt gaaattatgg atgatccttt catcaatagc atcgaaaaat
     9241 atttggctat agaaaaagag attgactctc ccttcctctt tgtatatcca gatattggta
     9301 atgtgtctgc atggcataat gatatctata gtgagtttta tcttggtcat catgccatcg
     9361 cagctctcca tctcaaggat acttatgcag tgacagaaag ttcaaggggc cagttccgag
     9421 atgtaccttt tgggcaaggt tgtgtcaaat gggaagaagc tttcgatatt ttaaaggaaa
     9481 ccaattataa tggacctttc ctaatcgaaa tgtggtctga aaattgtgaa acagtagaag
     9541 aaacacgcgc agccattcaa gaggcgcaag cttttctcta tccactcatt aagaaagcag
     9601 gtttgatgta agatgaatca agtaatcaat gctatgcgta aacgagtctg tgatgccaat
     9661 caatcattgc caaaacatgg acttgtcaaa tttacctggg ggaatgtatc tgaagtcaat
     9721 cgcgaactcg gtgtcattgt tatcaaacca tcaggcgtgg attatgacga attgacacct
     9781 gaaaacatgg tagtgactga tctagatggt aagatcctag aaggggattt aagaccatct
     9841 tccgacctcc caactcatgt gcaattatat aaggcttggt cagaaattgg tagtgtggtt
     9901 cacacccatt cgacagaagc tgttgcttgg gctcaggcag gtcgtgatat tcctttctac
     9961 ggaacaaccc atgcagatta tttctacggt tcaatccctt gcgcccgtag tttgaccaag
    10021 gacgaagtag aagtggccta tgaaaaagat actggcctgg ttatcgtaga agagtttgaa
    10081 catcgcggac ttaacccggt tgaagtacca ggaattgttg tacgcaatca cggtccattc
    10141 acctggggca aaaatccaga gaatgctgtt tatcactctg tcgtactaga ggaagtatca
    10201 aagatgaatc gctttacaga acaaatcaat ccaagagttg gacctgctcc ccagtacata
    10261 ctagaaaaac actaccaacg taaacatgga ccaaatgctt attatggtca aaagtaagaa
    10321 cgatgaagga ggagaaaaag ataaatttag ctcctctttt tacatttgat ttttattgag
    10381 agtaaagttg gagttgaagt aattttaaaa gattttttag aaatagcgct tgatatatat
    10441 atggtaaaat aaaaagaatt gctgtgatat caatagattt gggggatttt ttaatatggt
    10501 actggataag gcaagttgtg atttgcttca atatttgatg gatcaagaaa cgtccaaaac
    10561 gattatggcg atttcgaaag atttgaaaga gtcaagaagg aaaatttatt atcacattga
    10621 caaaatcaat gctgctctgg gtgacgaggc gcttcacatc attagtattc cacgaattgg
    10681 tattcactta acggaagagc agagagatgc ttgttgtaaa ctattatcgg aagtagattc
    10741 gtacgattat atcatgagtg cgcatgaacg tatgatgata atgttactat ggataggtat
    10801 ttctaaagaa cgtattacga ttgaaaaatt gatagagtta acagaggtat ctaggaatac
    10861 tgttctcaat gatttgaata gtattcgtta tcaactaact ttggaacaat atcaggtgat
    10921 cttgcaagtg agcaagtcac agggatacca ccttcatgcc caccctctta ataaaattca
    10981 gtatcttcaa tcgcttctat atcatatttt tatggaagaa aatgccactt ttgtatctat
    11041 tttagaagat aagatgaaag agaggttaga tgatgagtgt ttgctttctg ttgaaatgaa
    11101 ccaatttttt aaggaacagg ttcctttagt tgaacaagat ttagggaaga aaataaacca
    11161 tcatgaaata acttttatgt tgcaggttct accttatttg ctgttaagct gtcataatgt
    11221 tgaacagtat caagaaagac atcaggatat agagaaagaa ttttctttga taagaaaaag
    11281 aatagagtat caggtgtcta agaaattagg agaacggttg tttcaaaagt ttgaaatttc
    11341 tttgtcagga cttgaagttt ctcttgtagc tgttctcctc ctctcctatc gtaaagattt
    11401 ggatattcat gcagaaagtg atgattttcg gcaattaaaa cttgctttag aagaatttat
    11461 ctggtatttt gaatcacaaa tccgaatgga gattgagaac aaggatgatt tgttacgaaa
    11521 tttgatgatc cactgtaaag ccttgttatt tagaaagact tacggtattt tttctaaaaa
    11581 tcctctaaca aaacaaattc gatccaagta tggagaatta tttttagtca ctagaaaatc
    11641 tgcggaaatt ttagaaggag catggtttat tcggctaaca gacgatgata ttgcctattt
    11701 gacgattcat attggaggat ttttaaaata tacaccatca tctcaaaaaa atatgaaaaa
    11761 agtttatctc gtttgtgatg aaggtgttgc ggtttcgaga cttttgctga aacaatgcaa
    11821 actttatttt ccaaatgagc aaattgacac tgtatttaca acagaacaat ttaagagtgt
    11881 ggaagatatt gcacaagttg atgtagtgat tactactaat gatgatttgg atagcagatt
    11941 tccgatttta agggttaatc ctatccttga agcagaagat attttgaaaa tgctagacta
    12001 tcttaaacac aatatatttc gtaataagag caaaagtttc agtgaaaatc tttctagtct
    12061 tatttcgtct tatattgtag acagcaagtt ggctagtaag ttccaagaag aggttcaaac
    12121 acttataaat caagaaatag tagttcaagc ttttttggaa gatatttgaa ggacagtcca
    12181 atgatgaaca caaacctgtg tttttcttgg tcttttttag tgttttgaag ggtggtatac
    12241 taatctcaaa gataacaatt atatccaaag gaggcaacat atgccaaacg tcaaagaaat
    12301 tacaagagag tcatggattt tagccacttt cccagagtgg ggaacatggt tgaacgaaga
    12361 aatcgaagaa gaagtcgtac ctgaaggcaa ctttgccatg tggtggctag gcaactgtgg
    12421 tacttggatt aagacaccag ctggtgctaa cgttgtcatg gacctttggt caaaccgtgg
    12481 aaaatcaacc aaaaaagtga aagatatggt tcgtgggcac caaatggcaa atatggcagg
    12541 tgttcgtaag ctgcaaccaa acttgcgtgt tcagccaatg gttatcgatc catttgctat
    12601 caacgaacta gactattact tagtttcaca cttccacagt gatcatatcg acccatacac
    12661 agctgcagca attctcaata atcctaagtt agagcatgtt aagtttatcg gtccttacca
    12721 ctgtggacga atctgggaag gatggggtgt tccaaaagaa cgtatcatcg ttgttaaacc
    12781 aggtgacact atcgaattaa aagatatgaa gattcatgca gtagaatcat ttgaccgtac
    12841 ttgcttggta actctcccag tgaacggtgc tgatgagaca ggcggtgaac ttgctggctt
    12901 ggctgttaca gatgaagaaa tggctcaaaa ggctgttaac tatatctttg aaacaccagg
    12961 tggaaccatc tatcatggtg cagattctca cttctcaaac tattttgcaa aacatggtaa
    13021 agactttaaa attgatgttg ctttgaataa ctatggtgaa aatccggtag gtatccaaga
    13081 caaaatgaca tctatcgacc ttcttcgtat ggcagaaaat ctgcgtacca aagtcattat
    13141 cccagttcac tatgatatct ggtctaactt catggcttct actaatgaga ttctagaact
    13201 ttggaaaatg cgaaaagatc gcttgcaata cgatttccat ccatttatct gggaagttgg
    13261 cggtaagtac acttatcctc aagatcaaca cttagtagaa taccatcatc cacgtggttt
    13321 tgatgattgt tttgaacaag actctaacat tcaatttaaa gctttgctat aatataaaaa
    13381 tattctttgg aggttatccg tatgttgcat gatacggata attttcatat aatagtaaaa
    13441 tagaatgtgt gattcaataa tcacctcaaa tagaaaggaa attctatgtc aaatctatct
    13501 gttaatgcaa ttcgttttct aggtattgac gccattaata aagccaactc aggtcatcca
    13561 ggtgtggtta tgggagcggc tccgatggct tacagcctct ttacaaaaca acttcatatc
    13621 aatccagctc aaccaaactg gattaaccgc gaccgcttta ttctttcagc aggtcatggt
    13681 tcaatgctcc tttatgctct tcttcacctt tctggttttg aagatgtcag catggatgag
    13741 attaagagtt tccgtcaatg gggttcaaaa acaccaggtc acccagaatt tggtcatacg
    13801 gcagggattg atgctacgac aggtcctcta gggcaaggga tttcaactgc tactggtttt
    13861 gcccaagcag aacgtttctt ggcagccaaa tataaccgtg aaggttacaa tatctttgac
    13921 cactatactt acgttatctg tggagacgga gacttgatgg aaggtgtctc aagcgaggca
    13981 gcttcatacg caggcttgca aaaacttgat aagttggttg ttctttatga ttcaaatgat
    14041 atcaacttgg atggtgagac aaaggattcc tttacagaaa gtgttcgtga ccgttacaat
    14101 gcctacggtt ggcatactgc cttggttgaa aatggaacag acttggaagc catccatgct
    14161 gctatcgaaa cagcaaaagc ttcaggcaag ccatctttga ttgaagtgaa gacggttatt
    14221 ggatacggtt ctccaaacaa acaaggaact aatgctgtac acggcgcccc tcttggagca
    14281 gatgaaactg catcaactcg tcaagccctc ggttgggact acgaaccatt tgaaattcca
    14341 gaacaagtat atgctgattt caaagaacat gttgcagacc gtggcgcatc agcttatcaa
    14401 gcttggacta aattagttac agattataaa gaagctcatc cagaactggc tgcagaagta
    14461 gaagccatca tcgacggacg tgatccagtc gaagtgactc cagcagactt cccagcttta
    14521 gaaaatggtt tttctcaagc aactcgtaat tcaagtcaag atgccttgaa tgttgtggca
    14581 gctaaattac caacctttct aggtggatca gctgaccttg ctcactcaaa catgacttat
    14641 atcaagacgg atggacttca agacgatgcc aatcgcttga atcgtaacat tcagtttggt
    14701 gttcgtgaat ttgcaatggg aacaatcttg aacgggatgg cccttcatgg tggacttcgt
    14761 gtatacggtg gaactttctt cgtcttctct gactatgtga aggcagctgt ccgcttgtca
    14821 gccttacaag gacttcctgt gacttatgtc tttacccatg attcaatcgc agttggggaa
    14881 gatggtccga ctcatgaacc agttgagcat ttagcaggtc ttcgtgctat gccaaatcta
    14941 aatgttttcc gtccagcaga tgcgcgtgaa acgcaagcag cttggtacct tgcagtgaca
    15001 agtgagaaaa caccaactgc ccttgtcttg acacgtcaaa atttgactgt tgaagatgga
    15061 acagacttcg acaaggttgc taaaggtgct tatgttgtat atgaaaatgc agccgacttt
    15121 gataccatct tgattgcgac aggttcagag gttaatcttg ctgtctcagc tgctaaagaa
    15181 ttggctagtc aaggcgaaaa aatccgcgta gtcagcatgc catctacaga tgtctttgat
    15241 aaacaagatg cagcttacaa ggaagaaatt cttccaaatg cagtccgccg tcgtgttgca
    15301 gtcgaaatgg gtgcaagtca aaactggtac aaatatgttg gtctcgatgg tgccgttcta
    15361 ggtattgata ccttcggagc ctctgcccca gcaccaaaag tattggcaga atatggcttt
    15421 actgtagaaa atcttgtaaa agttgttcga aacttgaaat aatcctaaaa atcagggcgt
    15481 aagctctggt ttttcttacc agaaaagtaa ggtacaatct tgtaaaagta gctgaaattt
    15541 gatatagtag tcctatgtaa aagacaaagg agaaaacaat gaatccaaat attacttttt
    15601 taatcatgct tgtaggtatg atggccttga tgttctttat gcaacgttct caaaagaaac
    15661 aagctcaaaa gcgtatggaa agcttaaata aactacaaaa aggctatgaa gtgattacaa
    15721 tcggtggact ttatggaaca gtcgatgaag tagatacgga gaagggaaca atagttcttg
    15781 acgtagaggg agtttacttg acttttgaac tagctgctat caagacagta ttaccgctga
    15841 aagaaacagc ttcactagaa ggcgcaattg aaaaataaga cgggatcacg aactcccgtt
    15901 tttctataaa agaaaggaaa tgggatgaaa aaattagtct ttgtctgtct gggaaatatt
    15961 tgccgtagcc ctatggccga gtttgttatg aaatcaatga cagataacta cgaaatccaa
    16021 agtcgagcaa cttcctcttg ggaacatggc aatccgattc ataaggggac tcagggaatt
    16081 tttcaagagt atgagattcc ttatgacaag aacaagacat cgcttcagat tagtaaggaa
    16141 gattttgaag cctttgatta tattatcgga atggacgctt caaatatttc tgacttacgt
    16201 cagatgtgtc cagtagactg tcaagataag atttactcat tttcatctga aagtgttcca
    16261 gacccttggt atacaggaga ttttgaagaa acctatcgac gtgttcaaga gggctgtcaa
    16321 gcttggttag aacgtttaga aaaggagagt taaggtggaa aatcttacga atttttacga
    16381 aaagtatcgt gtctatctga ctcgtccacg tttagagctt ttggcagtag ttaccattgt
    16441 tttctgtgct gtactcgtct tttttctaaa tattccagga aaaggtgtct taaaactcga
    16501 taatggaacg attgtttatg atggcagtct tgtccgcggt aaaatgaatg gccaaggtac
    16561 cattaccttc caaaatggag accaatatac aggtggcttc aacaatggag ccttcaacgg
    16621 aaaaggtacc tttcaatcta aagaaggctg gacctacgaa ggtgattttg taaatggtca
    16681 ggctgaagga aaagggaaac taacaacaga acaagaagtc gtttatgaag gaacttttaa
    16741 acaaggcgtt tttcaacaaa aataaagcct ccttatcaaa ggaggtatta ttagaatcgc
    16801 aaggtaagcg cttacctgca aatccctttc tttccaaatc cctcttccaa gcaagtttgt
    16861 gaaataaaaa atatttgaaa taaatttcac aaacttcaaa gataaaacct gataagaaaa
    16921 gaaaatgaga aaagtttcac aagagtttaa aaattctttg tgaaatgttt atgaaactgt
    16981 ttacaaagtt gaaataatgc gttataataa acttgtgaaa aaattaacaa aggatatcgt
    17041 tccttgaaag ctatggagga aaatatggct gataaaaaaa ctgtgacacc agaggaaaag
    17101 aaactcgttg ctgaaaaaca cgtagatgag ttggttcaaa aagctctagt tgcccttgaa
    17161 gaaatgcgta aattggatca agaacaagtt gactacatcg ttgccaaagc atcagtagca
    17221 gctttggatg cccacggaga attggcttta catgcctttg aagaaacagg acgtggtgta
    17281 tttgaagata aagcaactaa gaacttgttt gcttgtgaac acgtggtaaa caacatgcgt
    17341 cacactaaaa cagttggcgt tatcgaagaa gatgatgtaa caggattgac cctgattgct
    17401 gaaccagttg gtgttgtttg tggtatcact ccaacaacaa acccaacatc aacagcgatc
    17461 ttcaaatcat tgatttcatt gaagacacgt aatccaatcg tctttgcctt ccatccatca
    17521 gctcaagaat catcagctca tgcagctcgt atcgtccgcg atgcagctat cgcagctggt
    17581 gctcctgaaa actgtgtaca gtggatcact gaaccatcta tggaagcaac tagtgccctt
    17641 atgaaccacg aaggtattgc aacaatcctt gcaacaggtg gtaatgccat ggttaaagcc
    17701 gcatactcat gtggtaaacc agctcttggg gtaggtgccg gaaacgttcc agcttatgtt
    17761 gaaaaatcag caaacattcg tcaagcagca cacgatatcg tcatgtctaa atcatttgat
    17821 aacggtatgg tctgtgcatc tgaacaagca gttatcattg ataaagaaat ttacgatgaa
    17881 tttgtagcag agttcaaatc ttaccacact tactttgtaa acaaaaaaga aaaagctctt
    17941 cttgaagagt tctgcttcgg cgtcaaagca aacagcaaaa actgtgctgg tgcaaaattg
    18001 aacgctgaca tcgttggtaa accagcaact tggattgcag aacaagcagg atttacagtt
    18061 ccagaaggaa caaacattct tgctgcagaa tgtaaagaag ttggcgaaaa tgagccattg
    18121 actcgtgaaa aattgtcacc agttattgca gttttgaaat ctgaaagccg tgaagatggt
    18181 attactaagg ctcgtcaaat ggttgaattt aacggtcttg gacactcagc agctatccac
    18241 acagctgacg aagaattgac taaagaattt ggtaaagctg ttaaagctat tcgtgttatc
    18301 tgtaactcac cttctacttt tggtggtatc ggggacgttt acaatgcctt cttgccatca
    18361 ttgacacttg gatgtggttc ttacggacgc aactcagttg gggataacgt tagtgccatt
    18421 aacctcttga atatcaaaaa agtcggaaga cggagaaata acatgcaatg gatgaaactt
    18481 ccttcaaaaa catactttga acgtgattca attcaatacc ttcaaaaatg tcgtgacgtt
    18541 gaacgtgtca tgatcgttac tgaccatgcc atggtagagc ttggtttcct tgatcgtatc
    18601 atcgaacaac tggaccttcg tcgcaataag gttgtttacc aaatctttgc ggatgtagaa
    18661 ccggatccag atatcacaac tgtaaaccgt ggtactgaga ttatgcgtgc cttcaaacca
    18721 gataccatca tcgcactcgg tggtgggtct ccaatggatg ctgccaaagt aatgtggctc
    18781 ttctacgagc aaccagaagt ggacttccgt gaccttgtcc aaaaattcat ggatatccgt
    18841 aaacgtgcct tcaagttccc attgcttggt aagaagacta aattcatcgc gattccaact
    18901 acatctggta caggatctga agtaacacca tttgccgtta tctctgataa agcaaacaac
    18961 cgtaaatacc caatcgctga ctactcattg acaccaactg tggcaatcgt agatcctgct
    19021 ttggtattga cagttccagg atttgttgct gctgatactg gtatggacgt attgactcac
    19081 gcgacagaag catacgtatc acaaatggct agtgactaca ctgatggttt agcacttcaa
    19141 gccattaaat tggtctttga aaatctcgaa agctcagtta agaatgcaga cttccactca
    19201 cgtgagaaaa tgcataacgc ttcaacaatc gctggtatgg cctttgccaa tgccttccta
    19261 ggtatttctc actcaatggc ccataagatt ggtgcgcaat tccacacaat ccacggtcgt
    19321 acaaatgcta tcttgcttcc atacgttatc cgttacaacg gtacacgtcc agctaagaca
    19381 gcaacatggc ctaagtacaa ctactaccgt gcagatgaaa aataccaaga tatcgcacgc
    19441 atgcttggac ttccagcttc tactccagaa gaaggggttg aatcttacgc aaaagctgtc
    19501 tacgaactcg gtgaacgtat tgggatccaa atgaatttta gagaccaagg aattgacgaa
    19561 aaagaatgga aagaacattc tcgtgaatta gccttcctgg cttatgaaga ccaatgttca
    19621 ccagctaacc cacgtcttcc aatggtagac catatgcaag aaatcatcga agatgcatac
    19681 tatggctaca aagaaagacc aggacgccgt aaataattgt ttatcagtct agaagcaaga
    19741 caaaaactca atttgaggga aagatccagt aatttttcta tgataaaagg catcctatca
    19801 aggtttttga acacctgata ggatgccttt ttatgatatt gaggcctttt tgcccttttt
    19861 gaaaaactag aatagaaaca aaatatataa tagattgaaa ctggaatagt acacttctgc
    19921 ttctaaaaca ttgttagaa
//
LOCUS       DAVBCW010000026        19715 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVBCW010000026 DAVBCW010000000
VERSION     DAVBCW010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19715
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.026"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(52..1464)
                     /gene="sufB"
                     /locus_tag="VQ514_000826"
     CDS             complement(52..1464)
                     /gene="sufB"
                     /locus_tag="VQ514_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HET2499608.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(1514..1954)
                     /locus_tag="VQ514_000827"
     CDS             complement(1514..1954)
                     /locus_tag="VQ514_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HET2499609.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQDQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            complement(1941..3167)
                     /locus_tag="VQ514_000828"
     CDS             complement(1941..3167)
                     /locus_tag="VQ514_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET2499610.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQSASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPASARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            complement(3178..4440)
                     /gene="sufD"
                     /locus_tag="VQ514_000829"
     CDS             complement(3178..4440)
                     /gene="sufD"
                     /locus_tag="VQ514_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HET2499611.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKINYLERLESRGEGSAKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSYADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            complement(4468..5238)
                     /gene="sufC"
                     /locus_tag="VQ514_000830"
     CDS             complement(4468..5238)
                     /gene="sufC"
                     /locus_tag="VQ514_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HET2499612.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITRLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            complement(5388..5582)
                     /locus_tag="VQ514_000831"
     CDS             complement(5388..5582)
                     /locus_tag="VQ514_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HET2499613.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            complement(5610..7265)
                     /gene="dnaX"
                     /locus_tag="VQ514_000832"
     CDS             complement(5610..7265)
                     /gene="dnaX"
                     /locus_tag="VQ514_000832"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HET2499614.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNVGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            complement(7265..7762)
                     /locus_tag="VQ514_000833"
     CDS             complement(7265..7762)
                     /locus_tag="VQ514_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET2499615.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTTWDFTM
                     FEEKS"
     gene            complement(7848..9050)
                     /gene="rpsA"
                     /locus_tag="VQ514_000834"
     CDS             complement(7848..9050)
                     /gene="rpsA"
                     /locus_tag="VQ514_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HET2499616.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(9212..9283)
                     /locus_tag="VQ514_000835"
     tRNA            complement(9212..9283)
                     /locus_tag="VQ514_000835"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9249..9251),aa:Gln,seq:ttg)
     gene            complement(9293..9373)
                     /locus_tag="VQ514_000836"
     tRNA            complement(9293..9373)
                     /locus_tag="VQ514_000836"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9337..9339),aa:Tyr,seq:gta)
     gene            complement(9423..9653)
                     /locus_tag="VQ514_000837"
     CDS             complement(9423..9653)
                     /locus_tag="VQ514_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HET2499617.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            complement(9821..10465)
                     /gene="pcp"
                     /locus_tag="VQ514_000838"
     CDS             complement(9821..10465)
                     /gene="pcp"
                     /locus_tag="VQ514_000838"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HET2499618.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            complement(10480..11403)
                     /locus_tag="VQ514_000839"
     CDS             complement(10480..11403)
                     /locus_tag="VQ514_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HET2499619.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            complement(11400..12089)
                     /locus_tag="VQ514_000840"
     CDS             complement(11400..12089)
                     /locus_tag="VQ514_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HET2499620.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            complement(12154..12858)
                     /locus_tag="VQ514_000841"
     CDS             complement(12154..12858)
                     /locus_tag="VQ514_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2499621.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     SRTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSDM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGFPVVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKYIK"
     gene            complement(12914..13936)
                     /locus_tag="VQ514_000842"
     CDS             complement(12914..13936)
                     /locus_tag="VQ514_000842"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HET2499622.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGTGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            complement(14068..16539)
                     /gene="parC"
                     /locus_tag="VQ514_000843"
     CDS             complement(14068..16539)
                     /gene="parC"
                     /locus_tag="VQ514_000843"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HET2499623.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKNTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAVTRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            complement(16959..18902)
                     /gene="parE"
                     /locus_tag="VQ514_000844"
     CDS             complement(16959..18902)
                     /gene="parE"
                     /locus_tag="VQ514_000844"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HET2499624.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVINTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            19038..19679
                     /gene="plsY"
                     /locus_tag="VQ514_000845"
     CDS             19038..19679
                     /gene="plsY"
                     /locus_tag="VQ514_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HET2499625.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
BASE COUNT         5665 a         4487 c         3558 g         6005 t
ORIGIN      
        1 ggcgatgctg acgtggtttg aagagatttt cgaagagtat aaaatcaaat tttatccaac
       61 tgatccctcc atttcatagc taatcaagcg gttcagctca actgcgtatt ccattggaag
      121 ttcttttgta aagggttcta caaatcccat gacaatcatt tcagttgcct cagattctga
      181 caatccacgg ctcatgagat aatacaattg ctcttctgaa atcttagata ctttggcttc
      241 gtgttccaaa gccacttgcg agttgtgaat ttcattaaat ggaatagtat ctgatgctga
      301 caagtcatcc atgataatgg tatcacattc aatgtgggaa acagatttct tagagttctt
      361 gttaaaggtg acttgtccac ggtagtcaac ctttcctcca cctttagcga tggatttaga
      421 cacaatagac gagctggtat gtggagcatt gtgaatcatc ttagcacccg tgtcttggtg
      481 ttgccctgca ttagcaaagg cgatagagag catggtacca cgcgctcctt ctccatcaag
      541 ataaacagat ggatatttca tagtcgtttt ggcacccaag tttccatcaa tccactcaac
      601 agtggcatcc ttttgagcct tagcacgctt tgttaccaag ttatagacgt tatcagacca
      661 gttttggata gttgtataac gcatataagc tccgtccaaa gcaaaaattt ctacaatggc
      721 agcgtgcaag ctattgcttg aatatgttgg tgctgtacat ccttctacgt agtggacgct
      781 tgctccctca tcaacgataa tcaaggtacg ttcgaactga cctatatttt cgttattgat
      841 acggaaataa gtttgaagtg gaatatctac cttgacacct tttggcacgt agataaaagt
      901 tccacccgac catactgctg agttgagggc tgccaacttg ttatctgtcg gcggtaccaa
      961 cttcgcaaag tattgtttaa ataagtctgg gtattccttg agtgcggaat ctgtatctgt
     1021 aaagataata cctaattttt ggaactcttc cttcatgttg tggtaaacca cttctgactc
     1081 gtactgggca gaagcccctg ctaaataagc acgttcagct tctggaatcc cgatacgttc
     1141 aaaggtttct ttaatctttt caggtacatc atcccaagaa cgggctggtt tgtcagatgg
     1201 tttttggtag tagattaagt catcaaagtc aatctctgac aagtctgctc cccaagtttg
     1261 catgggcatt tttttgaagg tttcataaga cttcaaacgg aactccaaca tccactcagg
     1321 ctcaccctta gcagcagata attcacgaat aacaccttcg ttgagtcctt ttcctgtcga
     1381 taagacaggc tctacatcgt catggaaacc aaatttatat tcaccaaggt caattggttt
     1441 tggttctact ctttcttcag ccataatatc ctttcttcat ttctaataat tcataagaca
     1501 aaagaaactt gtcttactgt ttttcttgat tttcaattgt tttcttaagg gcattccaag
     1561 ctagggttgc acacttgatt ctttgaggga atttggcaac acctgacaag aatgccgcgt
     1621 ctccaagttg gtcttgacgc tcatcttttt gcccttgaac catttcagaa aaaatagtcg
     1681 ccagttctaa aatttcttgt ttggtttttc ctaaaacggc atctgtcatc atactagcag
     1741 aagcagttga aatcgtgcat cctgaattta gaaaagcaat atcttccaaa cggtcctctg
     1801 catcaaactt gacagagagg ttgatgacat ccccacaagt cggattgttg agactgattt
     1861 gctcagcgtc ttctaacttc ccttggtgat gtggattttt cgaatggtct gctaccactg
     1921 ccatataaag gctatctagt ttagaaagtg ccattgaaaa actcctttgt cttttgtagg
     1981 gcatcgacta gtttgtcgca atctgccttg gtattataga tataaaaact tgcacgagct
     2041 gatgctggga cttccaaata ctgaagcaag ggttgcgcac aatggtgacc agcacgaaca
     2101 gccactcctt cataatccag agccgtcgca agatcgtgag gatggagatc acctaggtta
     2161 aaggcaataa cacccgaacg ttgagccaaa tcctgagaac cgtaaatggt caatccctca
     2221 attgcctgca gttttggata gacgtacgca atcaattcct gttcatgagc ttcaacggca
     2281 tccataccaa tcttttccag ataatcaact gcagtcgcaa gtccaatagc tcctgccata
     2341 tttggcgttc cagcctcaaa tttccaaggc aattccttcc aactagcgga ttgctcgtag
     2401 acaaaatcaa tcatctcgcc gccaaattct actggagaca tttgctcaag atacttttct
     2461 ttgccgtaaa ggacaccgat accagtcgga ccagccatct tgtgacccga aaaggcgaaa
     2521 aagtccagat ccaagtcctg gacatcaatc ttcatatgag gtgtagattg agcaccatcc
     2581 actaccatga ttgccccaac ttggtgggct aattgagtga tttccttgat cggattgacc
     2641 acaccaagaa cattggaggc atgagctagg gaaacaaatt taaccttatc agtcaattta
     2701 gctcgcaaat cctccatatc caaggcaccg tctttaagat agacatagac aagctctgct
     2761 ccagtctttc gacaagcttc ctgccatgga atgatattag aatggtgttc cattactgaa
     2821 atcaagacct ggtctccctc agtgagaatt tcctcagcaa agcgtgccac ccagttaagg
     2881 ctggttgtcg ttcctctggt aaagagaact tcctttgtag agcctgcatt aataaactta
     2941 cgaatggttt cacgagcagc ttcataagaa gctgtcgctc gttccgctaa ggtatggaca
     3001 ccacggtgaa cattggcatt gtcctgctca tagtaactgt taatagcttt cagaactact
     3061 agtggttttt gtgtcgtcgc agcattgtcc agatagacca gaggttcatc attgacaatc
     3121 tgatctaaaa ttggaaaatc cttgcgaatc gcttctacat ctaacatagg cttcccctta
     3181 gcgttttgac aatttctctt cgatagttgc aatcatttca tcacgaactt ccttgactgg
     3241 aatctccacg ataacagatc caaggaaacc acgaacaacc aaacgctctg cagttgcctt
     3301 atccaatcca cgactcatga ggtaatacat gtcttctgga tcaacttgtc cgatagacgc
     3361 tgcgtgtcct gcagtgacat cattttcatc aatcaaaaga attgggttag catctgaacg
     3421 cgcttggtct gaaagcatga gaacacggct ctcttgttgg gcatctgctc ccttagcacc
     3481 cttgatgatg tggccgatac cattgaaagt caaagttgct ttttcaagga taaccccatg
     3541 ttgtaggata tttccgatag agttgcagcc atagttagtt acacgagtat caatcccttg
     3601 tacctgacga ccacttgaaa gagctacaac cttgaggtca gcatagctac cattaccaat
     3661 caagtcacta tcaaaatcag caacgacatt tccttcgttc atgacaccga tagcccagtc
     3721 aatacttgca tcgttgccta atttaccacg acggctaatg taggcagtga cgttttcacc
     3781 tagacggtcg atagcagcaa acttgacttg cgcacctgaa cgtgcaatca cttctactgt
     3841 gatattagca gttgctttgg cacttccttc accgcgtgac tctaaacgct ccagataatt
     3901 aatcttagaa tttttaccag cgataatcat aatatgcttg ttaaacggca cattgctatc
     3961 gctatcttgg tagaaaattc cttcaattgg ctctgtgatt tctacgttat ctggaatata
     4021 gagtacagca ccactgttaa agtaagctgt gtggtaagcc gccaacttgt catcatcata
     4081 cttaacagat gacatgaaga attcttcgat cagctctgga atttcttcta aagctgagtg
     4141 aaagtctgtg aagacaacac cctgttcagc taactcaact ggagtttgtt cgaaaacagt
     4201 ttgagttcct acttgcacca acttcaagtg atgatctaaa gctgtgaaat ctggaacatt
     4261 tgctgatggc tcattttctg taatcgttcc atcacccaga ttccaacggt ggaatttgac
     4321 acgctcaata actggtaatt ccaaagtctc aatcttgtca aaagcttttt gacggagatc
     4381 agccaaccag cttggttcag cgtgcatttc tgaaaaaagt ttaatatttt ctctagtcat
     4441 ttacttctcc taaaagatac gaggaaatta caattcttcc ttgtagtcgt agccaagttc
     4501 ttcagctaat tttgcgtatc cttcacgttc caaacgcgca gccaattctg gaccaccaga
     4561 aaggacaaca cgaccttcca tcatcacgtg taccacatca ggtgtgatat agttcaaaag
     4621 acgttggtag tgagtgatga tcatagcacc aaaaccttca ccacgcatgg cattgacacc
     4681 tttagacaca actttaagag cgtcaatatc aagacctgag tcaatctcgt ccaaaagagc
     4741 aaatgttggc tccaacatca aaagttgaag aatttcattg cgttttttct caccaccaga
     4801 gaagccttcg ttgaggtaac gctctgccat ttcttctttc atgttgagca attccatttt
     4861 ttcatctagc ctagtaataa actcacgaac tgaaatcttc tcatcatctt ctttacccgc
     4921 attcatagcg gcacgaagaa actcagcatt ggtaattcca gggatttctg atgggtattg
     4981 catagcaagg aaaagtccca tacgcgcacg ctcatccact tccaactcaa ggatgtttac
     5041 gccatcaaac aaaacttcac ctttagttac ttcatagttt ggatttccca tgatagcggc
     5101 agaaagagtc gatttacctg taccatttgg tcccatgata gcggcaattt ctcctgtttt
     5161 cagggtcagg ttaacccctt ttaaaatttc ttttccttca atctcaacgt gaagatcttt
     5221 gatctctaat actgacatga tagttccttt ctttttcaaa acttttacag tacttactag
     5281 aaaaacgtct gatttcccca gaaatagagc aaaaattcta taaatatact tttctagtat
     5341 aacaaaaaaa agcctaaaag gctttgattt tgtctggaag ctgagggcta gtctttccta
     5401 ttaaaatgct gatcgatgac atcaacgatt ttgcccatca cataactgac tcggaaattt
     5461 ctaaagagta aaattgccca aaaggctgcc ataatatagc ggccagtcat ccaggcttca
     5521 ttgaaaaaat aggtctcagc agctgtaaac agcgccataa taataaattg ttgtttattc
     5581 ataaacttat tgtatcacga aatctttctt tagtcttcct ctaccttcac tttatcagcc
     5641 aaaaattcaa atccttctgg aatcaggctt tcttctactt ctttttcagt ttgagaagat
     5701 ttggctttgg ctgaaaaggc tgcgcgaact tctttccatt cctccatgga aatagctaaa
     5761 atctcaggtg aaaaacctgc cgcctgactg aggatattgc caaacatggt attgagattg
     5821 tctcgtttca tagtttgacc agcattgaag ttagactcaa aagcaagaat agcatggtgt
     5881 tcattggcag caaccggttg agaaccaact agcagagcct tgtccggccc acctagactt
     5941 tcaattacct ctccccaggc attctgcaaa cgaattagat tttgacgtgc taaatcagga
     6001 ttttcgacgg cctcttgtaa gatagattgc actttattgc gatcgacacg atagactgtt
     6061 ttgcccgtag ctggtcgact aggagctggt gcaacttgtt taggaaccgc acctacatta
     6121 gaaagctctt gtttgagacg ggcaacttcc tgtctcagcg tagcaatttc attttcaacc
     6181 gctcctgata gagctggttc gggcttgatt tccgccaaac ggacggtcat catttcagca
     6241 taaatcttgg gctgcaaact agacttaata tctgctaaac tcactgttgc taagcgaatc
     6301 atttcaaaca gatttttttg aggaagtgcc aaattttcta caaagactga actatgatga
     6361 gtattttctc cccctgtttg aacaattaac aagtctctta aatagtgcaa aagatcggtc
     6421 acaaaacgag tcatgctctt accattgtca aaaagaagat tcaagcaaga caaagctttg
     6481 ggaacatcct gttgagacaa ggccgccaca taatcatcca aggctgatag gctaatagtg
     6541 ccagtaattt cttcagagat agcagtcgtc agctcatttc cctgtgtcaa actcagggct
     6601 tgatccaaaa tagacaaggc gtcccgcatt ccaccttccg cccgtctggc aatgatttcc
     6661 acagcctctg gttcagaact gatattttct ttttctaaga tatagtgaat atgttcctta
     6721 atatcctgtg tcttaattga tttaaactca aaacgttgca cacgggatag aatagtagca
     6781 ggaatcttgt gtaattcagt agtggccaaa ataaagacta cattctgtgt tggttcttcc
     6841 agcgtcttta ggagggcatt aaaagcccct gtagacagca tgtgaacctc atctatgata
     6901 taaaccttat aacgagcaag gctaggcgca taggtagatt tatcacgaat ttcgcgaatt
     6961 tcatctaccc cattattaga agctgcatcc atttcaatga catcttctaa actaccgtcc
     7021 gtcactgctt gacaaatata gcagttattg caaggttcgc cacccacttg attgggacag
     7081 ttcatagcct tggcaaagat tttagcaaca ctggtttttc ccgttccacg aggaccagaa
     7141 aaaagataag cgtgacttat tttctcttgc tccaccgctt gtttaagagt cttagccaca
     7201 acttcttgac caactaactg ggagaagttt tgacttctat attttcgata aagtgcttga
     7261 tacattaaga tttttcctca aacatcgtaa agtcccatgt tgtcttttca agcaaaatag
     7321 cgacaaattg ttccaaataa tctcgatcca tagcatcgta atcctcaatc tctgaagaat
     7381 ccagatccag aactccaagt aactgaccat tcttcatcat cggcaccaca atttcacttt
     7441 tagctagact atcacaagaa atatagttga gataggtcgt cacatctcca acaataacag
     7501 tttcctgaaa gtgagctgcc tcaccacaaa cacccttgcc tagtgcaata cggatgcagg
     7561 aaacacctcc ttggaaggga cctaaaacca attcctttcc atcgaacaaa taaaagcctg
     7621 caaatacggt attaggaaaa cgtgatttta taagagcact ggcgttggaa agattagcca
     7681 aaacattggt ttcgccttcc aataggaagg acaattcttc atttaacatt tgataacgtg
     7741 attgtttttc tgattttaac atagaactat tatatcaaaa aaggagcaga gacaaaagaa
     7801 aaatcccttg tttagtaaac aaaggatttt gtgaattttc aatttgatta aagttcgata
     7861 tcaccaaaca aatcagccat tgaaaatcct gtttgtgttt ctggaagttc gaaatcacgc
     7921 ttttcttgac gtcttggacg acgtggacga gcagcacgtt tttcttcttt ttgtccttct
     7981 tcttgggctg gacgttcttc aagagcttta atagaaagtg acacgcgttc tgcatctgcg
     8041 ttaacttcaa gaactttaac ttgaacttct tgaccaactt taagagcttc ttttggattt
     8101 tcaatccgtt tgtgtgaaat ttgtgatacg tgaacaagtc catcgatacc tggcaatact
     8161 tcaacaaatg caccgaagtc agtcaaacgt ttaactgttc cttctactac atcaccttta
     8221 gccaattttt gctcaacgcc atcccatggt cctggtactg ttgctttaag tgaaagtgat
     8281 acacgtcctt cttcttcgtt aagatcaagg attttcactt caatttcttc accaacagtt
     8341 acaactgatt ttggtgatac gttacgttca tgtgacaatt cagtcaagtg aaccaatccg
     8401 tcaacaccac caaggtcgat gaaagcgccg aagcttgtga tacgagcaac tttaccagtt
     8461 acaacatcac caacagccaa tttaccgaat acttcagcgc gagctgctgc agtagctgct
     8521 tcaacaactt cacgacgtga aaggatgaag cggttttctt tagcgttaac ttctttgatt
     8581 ttagtatcaa attcttgacc tacaaaacgc tcagcgttac gtacgaaacg agtatccaac
     8641 attgaagctg ggataaatcc acgaacacct tcaaattcta ctgaaagtcc acctttaacg
     8701 gcacgcgttc ctttaacagt aacaacttct tcttcgcgac caacaagttt gtcccatgct
     8761 ttgcgagctt caaggcgttt tttagataca aggtatgtaa ctgtatcagt atctttacca
     8821 actacttgac gaagtacaag aacatccaat acttctccta ctttaacaaa gtcattgata
     8881 tctgcatcac gatcgtttgt caattcgcga agagtcaaga caccttcaac accagttcca
     8941 gagattgcaa cgttagcttg agtcgcatca actgtcaata cttcagcact aacaacatca
     9001 ccagtctcaa cttgactaac gctatttagc aaatcttcaa attcgttcat ctaaaaaatc
     9061 ctccaacaat caagtttttc ttaaacttga catacttata atttttttcc taagcacccg
     9121 caaggacatg ttcatatcat tcacgtcttt caattataaa aataaaatac cctaagatat
     9181 tttgcttttt atcttgatta ttacctaaga actggggtag ctggattcga accaacgcat
     9241 gagggagtca aagtcccttg ccttaccgct tggctatacc ccattaatga aatggagaga
     9301 gagggattcg aacccccgaa cccgaaggag cggatttaca gtccgccgcg tttagcctct
     9361 tcgctatctc tcctacaatc aacatggact attatatcat gaaaatttca aaaaaacaag
     9421 acttattttg ctaaattata attttcaatc aaaatttcca cagctttttc caatttttta
     9481 taaaaactat cgacattccc attctttata atggcaaatt gtccgtctaa ttcggcaatt
     9541 tctccgataa cttttttacc gatagtcaat gtataacctt caaaactgtc tttaccaaca
     9601 ttaactttgg catccgctac ttgaatttcg atttttttat ctttcttgct catttcatac
     9661 ctctcttcac tttttctatt ttacaagaaa atcctaaaac tagcaagaaa aaactctata
     9721 tcaggcttct ctgatataga gttttatgta aacaacctca attgatttag catagttaat
     9781 agttagtaaa caagcataat tgactatcct ttttaggtta tcagtgagtc tcaccgccta
     9841 ctaactgcaa atccttatct ttgtagtcta caatagcttc aatagctacc tctatgcctc
     9901 ttacaatatc acatagactc attcctgcag tattcggctt atttactact tgttccatca
     9961 tatatggaat atgcatgaaa cctgctctca tattagggaa ttttttatcc actaaataaa
    10021 gagcttggta catcaaatga ttgcaaacaa aggttcctgc actattggat acaactgccg
    10081 gaagtccctg ttttttgata gcttgtacca tcgctttgat aggtaaacta ctaaaatagg
    10141 ccgatgctcc atcaatacga atcggtgtat caattggttg attgccttcg ttatcaggta
    10201 tgcgagcatc atcttgatta atagccactc gttcaggtgt taagccggtc ctgccgcctg
    10261 cttgtccaat acaaagtaca gcatctggtt gatatcgtaa tatttctgcc tctaaaactt
    10321 ctgacgactt ataaaaaacc gttggaattt ctacccagcg aacttcagcc ccattaatct
    10381 cagatggtaa taattttaca gcctccaaag ctggattaat cttttcacct ccaaaaggat
    10441 taaaacctgt aaccaatatt ttcattttat tttcctttac taaaatgcga gaaagtacat
    10501 taagaatatg tgaataacaa tcattactag agcaacacct gcttgagcct ttataacgcc
    10561 attctgatct ttcatatcca tcaatgctgc tggtagagcg ttaaaattag cagccattgg
    10621 ggtcaataag gtcccacaat aacctgctgt catggcaaga gcaccagcca caattggatt
    10681 agctcccaga gcaaatacaa agggaactcc aacacctgct gtaataacgg tgaatgctgc
    10741 aaaagcattt cccataatca ttgtgaatag aaccattcca agaacataag ccaaaactcc
    10801 tataaagcga ctatctgaag gaacaatacc gctaatcaga tgagagataa catcaccaac
    10861 acctgctaca gtaaaaatag cccccaaagc ccctaataat tgaggaacaa tcccacttgt
    10921 tgaaacttgc tgagtcattc gattattttc tgataacaga ctcttagggt gactattggt
    10981 aatcacaaga acagaaattg tagcaaacaa ggcggcaagg ctaatcgaaa tcttgctaaa
    11041 ttctggaatc atttgcgcta agaccaacgc aagtattgcc atcagcataa ctggaataaa
    11101 aattttattt ttcaacctgt tagattcaat attggctttc atttcatcta aggatggcaa
    11161 ggttccgata cggacttgct taaacaatgt taacagcgat aataggatta caataatacc
    11221 aatactcata tttggcatat aggaaccacc tataaacgta atagacaata gagtccaaaa
    11281 tgcagatgtc ccaagtcgaa ctgggtttgt tttatcttta taactacaat aggctgtatg
    11341 gagaaattga caaccaatca caatataggt caactctaat agttgctttg ccaactctgt
    11401 catttttgtt ctcctcccct agtctttttt gatatcaatt ttttatcaaa taaataattg
    11461 taaatcccca ctacaataag tgttataaca gcaacaataa tagatgtaga agcaatccct
    11521 gcataattgc tttcatagcc taactgatct aatgttcccc ctatcaagag gactccccca
    11581 gcacctacaa acgtattttg agcaaagaaa tttccaaaat tttcattcgc agccgcacgc
    11641 gcttttattg tctcatcttc aacctctgtt aactttctac ctaattgaga ctctgcagct
    11701 gcttctccca taggttgaac caaaggtctg acaaactgag ggtgtcctcc tagacgaatt
    11761 gaaaagaaac cagctaactc tcgaataaag aaataaactg tatagaagtt tccaactgtc
    11821 agacctttaa tctttcgaat caaatcgatt gatcgttgct tgagtccaaa ggtttctgac
    11881 agccccacaa gaggcaaggt aaccataaaa atcgtgagca ctcgctgatt gctaaattct
    11941 tttcccaaaa tctccaaaaa ttcaacgaga gaaacacctg aaactaaagc tgtaaccaaa
    12001 ccagctaaga ctactgttgc aattgtatca aattttaaaa taaaacccac aacaatgatt
    12061 gctattccta ttaatctaat ccactccata tcaaactcct ttatattcaa aatgacagta
    12121 ttttaaaaat tttatcaaga tcaataccat tccttattta atgtattttt ctagttcttt
    12181 ttggtatttg ctattggatt ccaatttttc tttttgccat tttttaaaaa cctcgttata
    12241 ttcttttgtt gtaacaatat ctttttgcaa tttcattcct ttaaagatat atggttcccc
    12301 cttaatacca acttgtgagt atggttttga gaatggtact acgttactta caactggaaa
    12361 accaccagat gaagctgttg gcatcaataa tgaactatct gtcgaccaag cttgagcttt
    12421 ggcatatttt tcatatctct tctctaggtc agtggtctca gaaacagcat cttctaacaa
    12481 tttcttatat ttatccaaac caggtttagc tacaacatcc ttatcttttc ctttcgtaat
    12541 gccaaggtgt ttcatgtcag aaccagattt tggatccata atattcaagt aagacgctgg
    12601 atcttgatag cttggagccc atcctgtact gttcaaatca tagtcttttt gagaaggagc
    12661 aacattgccg tatttatcat tttccatcaa accatcaata acatttccaa taacgtctgt
    12721 cctcgatgtt cgagtcgcta tactgtagcc caatgatgct ggatctactg catagacata
    12781 agaaaatgtt gtcggtgcat ctgcttcttt atcagttttt ccacaagcca ctaaaatagc
    12841 tgacgtgctc aggaccactc ctgctgttaa gagccacttt ttctatttca taaaaaatct
    12901 cctttggttt attttaatct acttttacaa tccaaccttc tggcgcttca atatcgccaa
    12961 actgaatacc cgtcaattca ttatataatt tacgcgtcac aggacctact tctgtttcac
    13021 tatagaatac atggaaatca tcaccatgtt gaatacctcc aattggagaa ataaccgctg
    13081 ctgtaccaca ggcacctgcc tctacaaaac ggtcaagatt atcaattgga acatcaccct
    13141 caataggagt taatcccaag cgatgttctg ccaaataaag caaggaatac ttggtaatag
    13201 atggcaagat agatggactc aatggtgtta caaattcatt atcagctgta attccaaaga
    13261 agttagctga tccgacttct tcaatctttg tatgagttga tgggtccaga tagataacat
    13321 ctgagaaatg acgtgacttg gccatttttc ctggtaagag acttgcagca tagtttccac
    13381 caaccttagc cgcacctgta ccatttggtg ctgcacggtc gtactcatcc tgaatcaaga
    13441 agttggttgg gaccaaacca cctttaaagt aatttccaac tggcatagca aagatggtga
    13501 aaatgtactc ttctgccggt tttaccccga taatatctcc gacaccaatc aaaagagggc
    13561 gaagatataa ggttccacct gttccgtatg gtggtacgta ttcttcattc gcacggacaa
    13621 ctgctttaca agcttctaca aacatgtctg tcggaacttg tggcatcaag agacggtcac
    13681 atgtacgttg cagacgttta gcattttcat caggacggaa cagttgaaca ctgccatcct
    13741 tagtacgata agctttcaaa ccttcaaatg cttgttgtcc atagtgaaga cttggagaag
    13801 actctgaaat atgcaaagtt gcatcctctg taagctctcc ttgatcccat tgtccatttt
    13861 tgaaatgagc aagatagcga taaggtaatt tcatatagga aaaaccgagg ttttcccaat
    13921 caatcgttac tgtcatattc cactccttat tctaaaaacc tatttcttat attctacact
    13981 atttttctaa aatagcaagt atattttgta attttcagaa aatttctcca ataaaaacca
    14041 gctcttagaa ctgattcttc atttcactta tttatcttca gtaactactt cctgaagata
    14101 agcgtcaaaa acttcttcat ctgaaatcgt gtcagaaatg aagcttccat tgctagtgcg
    14161 ttctgacaag ttcaagtctt gcaatcggct ttcatagatt gttcctttat tggattggac
    14221 aagcagagtt tggtcgttca catccacttc cgtactgaag aaatcgccaa caaatccttg
    14281 ctctgcaact gctcctgcca agaagacacg atgcggtttg tttttcaact cacgcaagac
    14341 ttgtaatcct cgtttggcac ggctggttgc tagaatttcc tcaatagaaa cacgtttcaa
    14401 gcttccacgc tgggtcaaga ggtagaagga cgaagtatta cagataaagc cagattggag
    14461 gacatcatct tctttcaaat tcatagcctt gacacctgct gccttagcac cgacaaccgg
    14521 aacctcttcg atattgaaac gcagggcata accattttga ctaaccaaga caacatcatc
    14581 tagtttaatc ggagccactg ctacaatctg atctgtatcg tctttgagct tagcatactt
    14641 gacagactta gatctatagg tccgccatgg agtgaattct tttcgctcta cccgtttgat
    14701 ttgaccaagg cgagtcactg caaagtaggt tgtcgcatcg tcaaactgat ccagtacttc
    14761 cacataaagg atttcttcat tcgtttcaaa gtttgtgatg gtttggctca gatgctctcc
    14821 gatgtccttc caacgaatat ctgccaactc atggattggt ctgtagatga catttccaag
    14881 acttgtgaac atcaagaggt gctgggttgt cttggcagat tgaacaaaaa tcaaacggtc
    14941 atcatcacgc ttgccaattt cttccaaggt ggaagccgca aaggaacgtg gactggtacg
    15001 cttgatgtaa cctgccttgg tcacgctgac gtaggtatct tcctcagcga taagactagc
    15061 tgtatcaatc tcaattgctt tcgcagtgtc ttctaaagaa ctcaaacgag gagttgcaaa
    15121 tttcttcttg acctcacgaa gttctttctt catgagattg tacatagtcc tttcatcacc
    15181 gataatagcc gccagcatag caatcttctc acgaagctct gcttcttctt cctgcaagac
    15241 aaccacatcg gtattggtca aacggtacag ttgcaaagtt acgatagcct cagcctgttc
    15301 ttccgtaaaa tcatagctaa ctttgaggtt ttccttggcg tccgccttat tctcagaagc
    15361 acggataaga gcaatgactt catccaaaat cgaaatcaca cgaatcaaac cttcgacgat
    15421 atggagacgt ttctcagcct tttctttgtc aaagcgtgaa cgcgccaaaa tcacttctcg
    15481 acggtgagcg atgtagctag acaggattgg aacaatccca acctgacgag gtgtgaaatt
    15541 gtcaatcgcc accatattaa agttgtagtt gatttgtagg tcggtgtact taaataagta
    15601 gttgagaaca agctcagtat tagcgtcttt cttaagttcg atagcgatac gaagaccatc
    15661 acggtcagac tcatcacgaa cctcagcaat cccagctacc ttgttattaa cacgaacatc
    15721 atcgattttc ttgactagat tggccttatt gatttcataa ggaatctcag taataacgat
    15781 ttgttcctta ccacctttta gcttttcaat ttcagtcttg gaacgaacaa ccacgcgccc
    15841 tttcccagtc tcataagctt tcttgatttc atcacgaccc tgaataatag cccctgtagg
    15901 gaagtctggt ccaggcaaga attccatgag tttatcaatc tttgcagttg ggtggtcaat
    15961 catgtaaact gcagcatcta tgacctcagc taaattatgg ggaggaatgt ctgtggcata
    16021 accagccgaa atcccagtcg aaccattgac caagaggttt ggaaaggctg ctggcaagac
    16081 cgttggttct ttctccgtat cgtcaaagtt ccatgcaaaa ggaactgtat ttttctcgat
    16141 atcctgaaga aggtaacctg caatttcaga caaacgtgcc tcagtataac gcatagccgc
    16201 aggaggatct ccgtccatag aaccgttatt accgtgcatt tcaactagaa tctcacgatt
    16261 tttccagttc tgtgacatac gaaccatggc atcatagata gaagaatccc cgtgtgggtg
    16321 gaaattcccc atgatgttcc cgactgactt ggccgactta cggtagctct tgtcaaaagt
    16381 attgctatcc ttattcatag aataaagaat acggcgctga accggcttca acccatcacg
    16441 aatatctggc aaagcccggt cttgaataat gtacttggag tagcgaccaa agcgctctcc
    16501 catgatgtcc tccagggaca tgttttgaat gttagacata agatacaaag cccataaaat
    16561 accaagtgaa aatagaaaat tcttgaagta agcaaactca caagagaatt tatctttttc
    16621 acacagtatc tagggcgtgt tcaactcctt tcaaagaatg tagagtaggt ttttatgcag
    16681 taaaagatat tttacgggaa ttcctcccgt gttcagttac gataagtaac caaactatcc
    16741 tgtttgtatt tttcaatatg aaaatctggt tttccaaaat tagtcttagt ttgtgtctta
    16801 gccgctccct taagcgcctc tttgagataa gcactcatag cagattcttc attaataatc
    16861 ctgcaatttt ttcaaaccaa gattttcaaa ctgctttttc acatagtcat tcacatccga
    16921 ctctaatttc cagtttacta acatattatt ttctttcatt aaaacactgt cgcttcttct
    16981 agcgtaaact tgacattatc ttcaatccat ttacggcgtg gttctacctt atctcccatg
    17041 agaacattga cgcggcgttc ggcgcgcgct aaatcttcaa ttgtgacacg gatgagggta
    17101 cgtgtttctg ggttcatggt tgtttcccag agctggtccg cattcatctc accaagtcct
    17161 ttgtatcgtt ggagggtagc gcctttaccg aactgtttac ggagttcttc tagttctccg
    17221 tccgtccaag cgtaggccac ttcttctttc tttcctttac ctttggacat cttgtaaaga
    17281 ggtgggaggg caatatagac atgacctgcc tcgactagcg gacgcatgta acggtagaaa
    17341 aatgtcaaga gcaaggtctg gatatgggca ccgtcggtat ccgcatcggt catgataatg
    17401 atcttatcat agttggcatc ttcaatagag aagtctgctc caacacccgc accaatggta
    17461 taaatcatgg tattgatctc ttcatttttg aggatatccg ccatcttggc cttggctgta
    17521 ttgataacct taccacgaag aggtagaata gcctggaact tgcggtcacg accttgtttg
    17581 gcagaaccac cggcagagtc cccctcaact agatagagtt cattcttagc aggattctta
    17641 gattgggctg gggtcaattt cccagacaac aagcccttat ctttcttgtt tttcttccca
    17701 tttcggctct catcacgcgc cttacgtgct gcttcacgag catcacgggc cttgatagcc
    17761 ttgcggatga ggttagaagc taattcccca ttttccataa ggaaaaaggt caacttatca
    17821 gccactattc catccacaac tgggcgagct agggggcttc ctagtttatc cttggtctgt
    17881 ccttcaaact gcaagtgttc ttcaggaact aagatagaaa gaacggccgc tagtccctca
    17941 cgatagtctg aaccttcaag gtttttatct ttttccttga gaagacctgt tttacgtgca
    18001 tagtcattca tgaccttggt aatggcagac ttgagtcctg tctcgtgcgt tccaccgtcc
    18061 ttggtgcgaa cgttattgac aaaggataga atgttatctg agaatccgtc attgtactgg
    18121 agggctactt ccacttgaaa accattgtct tccccttcaa agtaaagaac tggcgtcaag
    18181 atttccttat cttcgttgag ataagaaaca aaatcttgta ctccattctc atagtggaac
    18241 tcaatcgctt catctgttcg cttgtccgtt aaagacaagg tcacattttt caagagaaag
    18301 gctgattcat taaggcgctc tgaaatggta ttgtacttga aatctgtcgt agaaaatata
    18361 gtcgcgtcag gcataaaagt aactttggtg cctgttttag acttgggtgc tgtaccgatt
    18421 ttcttcaaag tcgtgacagg ttttccacca ttttcgaaac gttgcttgta aactgcgcca
    18481 tcacgggtaa tttcaacttc taaccagcta gaaagggcgt taacaacgga agaacccact
    18541 ccgtgaagtc cacctgatgt cttatagcca ccttgaccga atttccctcc ggcatgaaga
    18601 atggtaaaga taacctcaac agttggaatt cccatagcgt gcatacctgt cggcatccca
    18661 cgtccatggt cttgaaccgt tagactaccg tctttattga tggttacatc gatacgatca
    18721 ccaaacccag acaaggcttc atcgactgca ttatcaacga tttcccaaac taggtgatga
    18781 agaccagcgc catcggtcga tccaatatac atccctggac gttttcggac cgcatccaac
    18841 ccttctagca cctgaatagc atcatcatta taattgttaa tattgatttc cttttttgac
    18901 acaaggaacc tcctattcgt tcatctttac tattctacag gttttccaag gattttgcaa
    18961 aatttttctt tctccgatgt gacaatttta gcagagattc tctgcttttc tttcccaatt
    19021 catgatataa taggagtatg attacaatag ttttattaat cctagcctat ctgctgggtt
    19081 cgattccatc tggtctctgg attggacaag tattctttca aatcaatcta cgcgagcatg
    19141 gttctggtaa cactggaacg accaatacct tccgcatttt aggtaagaaa gctggtatgg
    19201 caacctttgt gattgacttt ttcaaaggaa ccctagcaac gctgcttccg attatttttc
    19261 atctacaagg cgtttctcct ctcatctttg gacttttggc tgttatcggc cataccttcc
    19321 ctatctttgc aggatttaaa ggtggtaagg ctgtcgcaac cagtgctgga gtaattttcg
    19381 gatttgcgcc tatcttctgt ctctaccttg cgattatctt ctttggagct ctctatcttg
    19441 gcagtatgat ttcactgtct agtgtcacag catcgatcgc ggctgttatc ggggttctgc
    19501 tctttccact ttttggtttt atcctgagta actatgactc tctcttcatc gctattatct
    19561 tagcacttgc tagtttgatt atcattcgtc ataaggacaa tatagctcgt atcaaaaata
    19621 aaactgaaaa tttggtccct tggggattga acctaaccca tcaagatcct aaaaaataaa
    19681 atgccagttc tgtgaactgc accccaaaag ttaga
//
LOCUS       DAVBCW010000027        19548 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVBCW010000027 DAVBCW010000000
VERSION     DAVBCW010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19548
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.027"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(<1..777)
                     /locus_tag="VQ514_000846"
     CDS             complement(<1..777)
                     /locus_tag="VQ514_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2499626.1"
                     /translation="MSKETVVYTKRREKIKDNILGYSFLAPALILLGIFLVIPVGMVI
                     YYAFTDYYLLTPDERKFVGLENFIRLTKDPIFLKSFLNTLKFVVWIIPVQLGAALGMA
                     LIVNKKRKGNMFFKVAFFAPVVMSLVVISILWLYLLNPNSGLLNAILNKVGIASQPFL
                     TSPKQAMYAIVFVSAWQGAGYQMLLFLGGMQNIPQDVYEAAELDGFSKWAQFRYITMP
                     LLKPTALFVLLTTLISAFKLIVQPMVMTQGGPLNSTITMVY"
     gene            complement(873..2183)
                     /locus_tag="VQ514_000847"
     CDS             complement(873..2183)
                     /locus_tag="VQ514_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET2499627.1"
                     /translation="MRKGTMLSVVAGLSLAILAGCSGGTNSKQASSSNDIKVWVQFSD
                     ETNEGKAWQKVVDNFNQSGKSKYKVVTEYIPRSGSGGGYEDKVNAAITTNSLPDVITL
                     DGPNTAAYAKSKVITPLDDYLKDNNMDDVLDSIKQQGTYNGKFYAFGFSESNVGVYYN
                     KKMFKEAGIAESELPTLEKPWTWDEFNTIAKKLKDHYNKPAIDFRINSNDEMLPYAYM
                     PLIWSNNGSVVNEDGTKAEGYFNSKESVEAVQFIQNLVKEGYTTVSPVEKGFETGEYP
                     MLLSGSWTIADMNTNYKDIDFGILPYPVSSKTKKLVSPSGSWQLAVTTKSDKKEAASE
                     FVKFATNTESSEIISLGNSVLPIRKSTIENIKDKVSEPMRFLMEQNSKTAHARPVVVA
                     YPQVSRAFQQAMQDISYYEENPNVQKVLDTRTKEMQTAIDQSLK"
     gene            complement(2310..3305)
                     /locus_tag="VQ514_000848"
     CDS             complement(2310..3305)
                     /locus_tag="VQ514_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2499628.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLRKVQR
                     AIKTLDYIPDNYARGMKKNRTETIALIIPSIWHPFFSEFAYHVEKELLKYNYKLFICN
                     ADSDSQNEIEYIEMLKQNKVDGIIGITYSDIDKYILSDLPFVSIDRHFSENVCYVTSA
                     NYKGGQTAAEELVRRDNKHLAFIGSINMYENETILRSKGFREKAKELGVDVYNFEMKE
                     PLINPEDQIRIFLQSHKEIDGIFTINDMMALRVISVMKELGKIAPDDYQIIGFDGLKT
                     SVDQSYLVSTIVQDIGMMAKESVAMILKMIKREEILEKRIVIPTYFGGDKTTKKI"
     gene            complement(3667..5148)
                     /locus_tag="VQ514_000849"
     CDS             complement(3667..5148)
                     /locus_tag="VQ514_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2499629.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSS
                     YDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            5591..5755
                     /locus_tag="VQ514_000850"
     CDS             5591..5755
                     /locus_tag="VQ514_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET2499630.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            5839..6405
                     /gene="amaP"
                     /locus_tag="VQ514_000851"
     CDS             5839..6405
                     /gene="amaP"
                     /locus_tag="VQ514_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HET2499631.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            6417..6587
                     /locus_tag="VQ514_000852"
     CDS             6417..6587
                     /locus_tag="VQ514_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HET2499632.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            6618..7226
                     /locus_tag="VQ514_000853"
     CDS             6618..7226
                     /locus_tag="VQ514_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET2499633.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            7257..7460
                     /locus_tag="VQ514_000854"
     CDS             7257..7460
                     /locus_tag="VQ514_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET2499634.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(7570..7848)
                     /locus_tag="VQ514_000855"
                     /pseudo
     CDS             complement(7570..7848)
                     /locus_tag="VQ514_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895264.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8046..8333
                     /locus_tag="VQ514_000856"
     CDS             8046..8333
                     /locus_tag="VQ514_000856"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HET2499635.1"
                     /translation="MIVSLKTKSRKAKDMAESIQGWLAQFLVNLFKSITFDCGKEFSK
                     WKDISNHHDSESFFANLGCSRQRRLNEHSNRLLRCHDLPKQTDFNEVSQEF"
     gene            complement(8611..9381)
                     /locus_tag="VQ514_000857"
     CDS             complement(8611..9381)
                     /locus_tag="VQ514_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET2499636.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTIKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(9396..10244)
                     /locus_tag="VQ514_000858"
     CDS             complement(9396..10244)
                     /locus_tag="VQ514_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HET2499637.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDDFEGPQVAVSVKDFSIADTPV
                     TNQEFAQFVKETGYKTLAERQEWSFVFILFVPEAEREGYPHPAGAPWWLQVPNACWKH
                     PYGENSNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTALDRFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            complement(10241..11716)
                     /locus_tag="VQ514_000859"
     CDS             complement(10241..11716)
                     /locus_tag="VQ514_000859"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HET2499638.1"
                     /translation="MVQTKQPNIILIVVDQMRADALSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKDMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKAYGSQFDYVSDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDMYMERLPQFLDLHIGNWEVLEKQIPSIYALRGKLKEDDQRRMVAAYFGLI
                     THIDHQISRFLTSLKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHALGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVDFLRYREEGFVVDEELVPVELSKITPTLTKTGDSQS"
     gene            complement(12039..13394)
                     /locus_tag="VQ514_000860"
     CDS             complement(12039..13394)
                     /locus_tag="VQ514_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2499639.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGFFSTAFDWRAIILSVVCLIISVLTYFPFVKMADKTEL
                     S"
     gene            complement(13404..13721)
                     /locus_tag="VQ514_000861"
     CDS             complement(13404..13721)
                     /locus_tag="VQ514_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2499640.1"
                     /translation="MDESNLESVMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTREAQGEETSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            complement(13738..14055)
                     /locus_tag="VQ514_000862"
     CDS             complement(13738..14055)
                     /locus_tag="VQ514_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2499641.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            complement(14068..15753)
                     /locus_tag="VQ514_000863"
     CDS             complement(14068..15753)
                     /locus_tag="VQ514_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HET2499642.1"
                     /translation="MKNKKLISSLFILFLLIFIHVKVFANENLIKNSNFEQGELFWNS
                     RNRGIINSNESYSNGHYSGMIPSTAINDNRADGYIGQVIDIEPNEDYLVSAFAKVDIS
                     GAEGYFTARWFDNNKQGEIVQNNIGEAVDQTVNTTEWKKYMFSFNSGKHDKVLIQLVK
                     WSEDDTTKKSNIFIDNVEMYQLSKGNSYKKIWRDDFDGEQLNKKYWGYELGSIRGWEQ
                     QHYVRSDENVFLRSGNLVLRATNRSKEDQYFNPRNNHRKVVYNSGSVRTHGKVEFLYG
                     KLEMRAKLPKGQGVFPAFWTLGSDFTLDGKINPVQGRGWPSTGEIDIMELVGERNSNG
                     SGNKTVYQTLHYGQSDNDDGKFAGRGTAFKLSSGNFNDSYHTFSIDWYKDYIVWMVDN
                     HIVRKVYYGSDNIAKKIFNRPQYVQLNLAMGGSWPGVVDNNLSGTEFVVDYVSYSRND
                     LQQHQAEEYYSQSPKINGAKDIVINRGDVPDLLKGITTNSGYELDYSIENEPMFNNFG
                     GNTSVDLLIRNKTEKEKISQMPPGVYNLHYTSRPKHLDYESKVDRKTVTLTIK"
     gene            complement(15758..16654)
                     /locus_tag="VQ514_000864"
     CDS             complement(15758..16654)
                     /locus_tag="VQ514_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HET2499643.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLNRIEELGFDFVCLTGNPGTGVSNREIIKAVQTAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            complement(16657..17919)
                     /locus_tag="VQ514_000865"
     CDS             complement(16657..17919)
                     /locus_tag="VQ514_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2499644.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFIRGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLL
                     QIVLIVLDILLYLPFVIAIEKRFKLLED"
     gene            18199..19377
                     /locus_tag="VQ514_000866"
     CDS             18199..19377
                     /locus_tag="VQ514_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HET2499645.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDDYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALFYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
BASE COUNT         6655 a         3776 c         2826 g         6291 t
ORIGIN      
        1 gtacaccata gtaattgttg agtttaatgg gccaccttgc gtcataacca ttggttgtac
       61 aatcagctta aaagcagaaa ttaaagttgt taacaataca aacagggctg ttggttttag
      121 taggggcatc gtgatataac gaaactgagc ccattttgag aatccatcta attcagcagc
      181 ttcatagaca tcttgtggaa tattctgcat tcctcctaaa aataaaagca tctgataacc
      241 tgcaccctgc caagcagaaa caaaaacaat tgcatacata gcttgtttag gactagtcaa
      301 gaaaggttga gaagcaatac ctaccttatt taaaatagca tttaataagc cactatttgg
      361 atttaaaaga taaagccaaa gaatagaaat aactaccaag gacatgacaa ctggagcaaa
      421 aaaagccact ttaaaaaaca tatttccttt tcgcttttta ttgacaatca aagccattcc
      481 taaggccgct cccaattgaa ctggaataat ccaaacaaca aatttcaaag tattcaagaa
      541 gctctttaag aaaatcggat cttttgttaa tcgaataaaa ttttcaagtc caacaaattt
      601 tctttcatct ggtgtcaaca aataataatc tgtaaaagcg taataaatga ccattccaac
      661 tggaattact aaaaagatac ctaataaaat taaagctgga gctaaaaaac tataacctaa
      721 tatgttatcc tttatttttt cacgtctttt tgtatacaca acagtttctt tactcatatc
      781 catctccttt aatcatatat atcaaactag gctattaaca agaaagggga aggtagttcc
      841 aaacattttc cttccacctt cttttttaca gattatttga gtgactgatc aatagcagtt
      901 tgcatttctt ttgtacgagt atccaagact ttttgcacat ttggattttc ttcataatag
      961 ctaatatctt gcattgcttg ttgaaaagct cgagaaacct gaggatatgc tacgacaact
     1021 gggcgagcat gagctgtttt tgaattttgt tccatcaaga aacgcattgg ttcggatact
     1081 ttatctttga tattttcaat tgtagattta cgaattggaa ggaccgaatt ccccaagcta
     1141 ataatttcac ttgattctgt atttgtagca aatttaacaa attcagatgc ggcctctttt
     1201 ttatcagatt ttgtcgtcac agctaattgc caactacctg aaggggatac taattttttc
     1261 gtcttactag atactgggta aggaaggata ccaaaatcaa tatctttgta gtttgtattc
     1321 atgtcagcaa tcgtccaaga accactcaaa agcattggat actctcctgt ttcaaaacct
     1381 ttttcgactg gactaacagt tgtatagcct tcttttacaa gattttgaat gaattgaact
     1441 gcttctacac tctctttgga gttgaagtat ccttctgcct ttgttccatc ttcgtttacg
     1501 acagaaccat tatttgacca aatcaacggc atataagcat atggcaacat ttcatcgttt
     1561 gaattaatac gaaaatcaat tgctggtttg ttataatgat ctttcaattt tttagcaatc
     1621 gtattaaatt cgtcccatgt ccatggcttt tctagcgttg gaagctctga ttccgcaatt
     1681 ccagcctctt tgaacatttt tttattgtaa taaacaccaa cattcgattc agaaaatcca
     1741 aatgcataga atttcccatt ataagttccc tgttgtttaa tactgtccaa aacatcatcc
     1801 atattattat cttttagata atcatctagc ggagtaatta ctttagattt tgcataagct
     1861 gccgtatttg gtccatcaag ggtaataaca tctggaagac tattagtcgt aatagcagca
     1921 ttaaccttgt cttcatatcc tccaccacta ccactacgag gaatatactc agttacaacc
     1981 ttgtacttac tttttcctga ttgattaaag ttatcaacaa ctttttgcca agcttttcct
     2041 tcatttgttt cgtcagagaa ttgtacccag actttaatat catttgaaga actcgcttgt
     2101 tttgagttag ttcctccact acaacctgca agtatagcta acgatagccc cgcaacaaca
     2161 gataacattg tacctttacg catcattttt ctcctttttg aaacgtttca ttttttattt
     2221 attttttaaa caagcttcta gcctattttt gaaacgtttc atttgttgat tatattataa
     2281 cagcgctttc attaattgtc aagcgctttt taaatttttt ttgttgtttt atctccccca
     2341 aaatatgtgg gaattacgat tcgtttttct agaatctctt ctcgcttaat cattttcagt
     2401 atcattgcaa cagattcttt tgccatcata ccaatatctt gaactattgt agacactaga
     2461 taagattgat cgacagaagt ttttaaacca tcaaacccaa ttatctggta atcatcaggt
     2521 gctattttcc ccagttcttt cataacacta ataactctca gagccatcat atcatttatt
     2581 gtaaatatac catcaatttc tttatgagat tgcaaaaaaa ttcttatctg atcttcagga
     2641 tttattaaag gttctttcat ttcaaaatta tagacatcta ctcctaattc cttagctttt
     2701 tctctgaatc ctttacttct gagtatagtc tcattctcat acatattaat tgaaccaata
     2761 aaagcaaggt gcttattatc ccttctaacc agttcctctg ctgcagtttg tcctcctttg
     2821 taatttgctg aagtaacata gcatacattt tccgaaaaat gtctatctat acttacaaac
     2881 ggtaaatcag ataaaatata tttgtcaata tcagaatatg taataccaat aattccatcc
     2941 actttatttt gtttcaacat ttcgatatat tcgatttcat tttgtgaatc gctatcagcg
     3001 ttacaaataa ataatttata attatatttt aataactctt tctcaacatg ataggcaaac
     3061 tccgaaaaaa atggatgcca tatgctcgga ataatcaaag cgatagtttc tgttctattc
     3121 tttttcatac ctcgagcata attatcaggg atatagtcca atgtttttat agccctttga
     3181 actttcctta aagttacttc tttaatgcct ttttctttat taattacacg tgaaacagtt
     3241 ccaacactaa ctcctgcttc taaagcaaca tctttcatgg taattgattt tctttgttct
     3301 accatattat cacctccttt caatatatag tatcatgcaa atgcttttta agcaactatt
     3361 tctcaatcat ttttggccag atcatttatc ccatcatgaa taaaatcact ccaattagct
     3421 tttgaaaata cttcaatttt catgtgtaaa catctacata aaacaggaaa agccttggtt
     3481 ttatggcttt tttcgtatct tctacaaaaa aagcaagagt tttagagggc tataaatcta
     3541 gatgtacatt ttgcttaaat gattgaaggt cttttcttaa caaaaacacc ccaaaaatta
     3601 gattttttct gtctaacttt tgaggtacag ttcaaacgcg aaatagcgtt tttttgttat
     3661 ttttggttac tcatctaatc gaataaacat catggcattt aacaaatata tgagtgagac
     3721 cgtgtttata ttatttgaat agatgagtct cttattttca ataggaggaa taataaaatt
     3781 agaaataatg atatcataag atgaatcttc taaagattcc tttgataatt ctaattcagt
     3841 ccaaacttcc agttcaaaat tattgctaca gtaataagaa agtgtctctg caacgaattt
     3901 tgcatgatac tgatcaaaat tactcataac caaaaccttt agtttaggct gattttgtag
     3961 caaattaatc accaaatgtt tggtatgagt gatgaaggta taagatagat gatttaccat
     4021 cattgaacta gaacaaacct caagagtctc taaatagtga gaaagctctt tttttatatc
     4081 tgaaacaaat tttggaaaaa tattttgaaa gttcctgatt gtattccctt tttgatcaaa
     4141 taaaataaac tcagtaaaca actcttgacg atacagatgt gcggtattat gcagatgcca
     4201 aatcagatta tccttattct ccatttcaat ctgatacttg actgaaatct gatcaataaa
     4261 atcactcaat agatggtaag atttttcaac ataactatcc ttttttacgc atttcataaa
     4321 gagactttca tctatgaaaa acattttttg aaagtaagac acaaataatt ggcaaacaac
     4381 ttcttcatct aaagagatat tgtattctga ttcaaaactc tgagcaacac cttctattcc
     4441 ttctgcctgc attaaaaaat ccaaactttg gtcgttaaaa gaatctttat ctacttccat
     4501 aaaatgacca aactttattc tatataggtt cgtaactagg agcaacttta gcattctatg
     4561 cgttgacaaa ttcattggaa agcttgtttc cttataaacc aattctaaca attgagatag
     4621 tggctctgat gaaaaatttt caaatggcca ttctaggaaa taatattttt ctgaaaaata
     4681 ttgtgcaaaa aagtaacgaa tgtctctctc atttccaatg atttgaacag gggtcagact
     4741 gacttcaaat tgaaattgcc ttttaatcac tttattgatt tggctaataa tacgatagag
     4801 cgaagatgaa ctgatataaa attctttaca aatactctca gcttgacaac cttcattaaa
     4861 gaagatgaat tctaaaatcg aaaaatgagt tgaatgttta aagaaatgat ggtaaaccat
     4921 ttcaatatca ctatcatcgg tattaataat gcgtatacca ttagtagaag aatgaaaaat
     4981 caagtcagga aaagcagatt taacatggga tagatcatct ttgactgcac gttctgtaca
     5041 atttaataac tctgctagtt cagaacgatg aaaccaacgt ttatgttcaa ataataattc
     5101 taataattct aattgcctat gacttttttt agataataaa tctctcatga atatctttct
     5161 ctctttataa attatcggat taaacctctt gcaattatac cacaaagaat aggtatagca
     5221 tgatataacg acttttccta aaatctttta tttcgtataa taacactacg gagacaatat
     5281 ataaacaatt ttcttatttt accgtctatt gagggcgtga atacagaatc aaattcaagt
     5341 ctaaagatta tatttttaat tttaaaaatt atataatagc aacaattaaa gaatttgatt
     5401 ttttaaaatt atataataat aacaatcgaa ataattgact tttctatatt aaagttatat
     5461 aatagtatta atcaaagaaa ttgatttttt gatattaaaa taaaaaagga gggtaggcag
     5521 tgttgtgatc aattattgct ggaggtctta ttggtctctt ggcaggtaaa atcactaaaa
     5581 aagtagttct atgggaatca tcgcaaatgt attcgctggt ttagtcgggg catatgcagg
     5641 acaatctctt ttaggtagtt ggggtccatc aatcgctgga atggctttgc tcccatctat
     5701 tgtaggtgca gcgattgtga ttactgtagt gtcattcttt acaggtagaa agtaaacttt
     5761 tcgccagtaa agttagcaaa ctatttttaa atcaatgacg ggaaaaatag tttaaatgtt
     5821 aaatcgaaag gattgtatat gtcaaaagca aagaaaatat gtttcattat tttctgtatt
     5881 ttaatcttga caattttcct tcctgttttg atagattatc atcaagttag tgatctaggt
     5941 attcatctac ttagctggag acagaactcc gtagttgaat tctatcttgc tagatatgtc
     6001 ttttggggga cagtggttct atcaacttta gttttattat ccattttagt tgtgatgttt
     6061 tatcctaaac gttacttgga aatccaactt gaaactaaaa acgatacatt aaaattaaag
     6121 aattcggcaa tcgaaggttt tgttagaagt ttggtgagtg atcatagatt gatcaagaac
     6181 ccaactgttc atgtaaattt acgaaaaaat aaatgtttcg ttcatgtaga aggtaaaatt
     6241 cttccttcag acaacatcgc tgacagatgc caaataattc aaaatgaaat aactaatgga
     6301 ttgaagcagt tttttggtat tgagcgtcaa gtaaaacttg aagttgcagt aaaaaattac
     6361 caaccaaaac ctcaaaacaa aaagactgtt agtcgtgtga agtaaggaag taaaaaatgg
     6421 aatggcttaa acaatatcga tatccaatta tcgctggtct cataggcgta tttctggctt
     6481 gtttgattgt ctcctttggc ttcttcaaaa caatatttgt attgatttta ggagcactgg
     6541 gagttgcagc tggattatat atcgaaaaaa actatataga taaataaaaa ttactaattt
     6601 aattaaagga gtttcatatg tcaaacgaaa aaaacacaaa tactaacgta gaaaagaaag
     6661 atgctactgt tgtagctcac gaaatcaaag gggaacttac ttacgaagat aaagttatcc
     6721 aaaaaatcat tggtctttca ctagaaaacg tttcaggtct tttgggaatc gatggtggtt
     6781 tcttctcaaa tcttaaagaa aaaatcgtta acagcgatga cgtaacaagt ggtgttaacg
     6841 tagaagttgg taaaacacaa gttgcagttg acttaaacgt tattgttgag taccaaaaaa
     6901 atgttccagc tttatattca gaaatcagag aaatcgtatc ttcagaagtt gctaaaatga
     6961 ctgacttgga aattgttgaa atcaacgtaa acgttgtcga catcaaaact aaagaacagc
     7021 atgaagcaga ctcagtaagc cttcaagatc gcgtatctga cgttgctgaa tcaacaggag
     7081 aattcacttc agaacaattc gaaaaagcta aatctggtct tggatctggt ttctcaactg
     7141 ttcaagaaaa agttagcgaa ggtgtagaag ctgttaaagg tgcagcaaat ggtgtagtat
     7201 ctcacgaaaa cactcgtgta aactaagata aaataaatat aacaggagaa attatcatgt
     7261 cagtagaaga aaaattaaat caagctaaag gttctattaa agaaggtgtt gggaaagcca
     7321 tcggtgatga aaaaatggaa aaagaaggag cagctgaaaa agttgtttct aaagtaaaag
     7381 aagttgccga agacgctaaa gacgctgtag aaggtgctgt agaaggtgtt aaaaacatgt
     7441 tgagtggcga cgataaataa ggttaaaagt tactttatct ttttggtaac attagtcaaa
     7501 agagtctgag tcaagatgat tctcagaaaa caaaaagcta gagattccca attgcggaac
     7561 tctagctttt taattttgcc tctttctctt attatatttc agcaggttgt tggccatgag
     7621 tatgaatccc atgtcaattc tcacttgacg cttacctctc agatgacatc tcttataacc
     7681 caaacaaacc tttatctgcc caaagacaga tttcatatca atcttacgtt tagcgaaaat
     7741 ttgtctaccc ttggaagata aaagtgcctg atattcttta gtttttaaac actggtaacg
     7801 ttcattcata tacagtccct tttgaggggc tgattcaggt tcataatcgc agtcaacatt
     7861 gatttcaagg ctgtttgctt tctatctccc ctttttcatg atatattttc gtacaggttc
     7921 ttcaatagtt tctatagtaa aatgaaccaa aaatagtaca cgttgcggta taatgttctt
     7981 gtggcatatt cattagattt tcgtaaacag ttctctccta tttgtgagcg aacaagtata
     8041 gtcaattgat tgtcagtttg aagaccaaga gtcgtaaggc aaaggatatg gcagaatcca
     8101 tccaagggtg gctggcacaa tttctagtga acctctttaa atctattacg tttgactgtg
     8161 gaaaagagtt ttcaaaatgg aaagatattt ccaatcatca cgatagtgag agcttttttg
     8221 cgaatctagg atgttcaaga cagcggcgtc tcaacgaaca ttcaaataga cttttaaggt
     8281 gtcatgactt gccaaagcag acagatttca acgaagtcag tcaagaattt taggctggac
     8341 aaaaactcaa tttcaggaga aaatgaaata aatattccca caataaaacg catagtatca
     8401 aagtttttcc aacacctgat attatgcatt ttttaatttt aaagactttt tcccagcctc
     8461 aagaaatcac atcccaccta aatcctttgg ataccaaata cctcatcagg tattttctga
     8521 gttggtaaga atgtctagct taatttgaca aatggagggc gttaaatcca gtttttacag
     8581 gaagtctatt taaatatttt cacactgacc ttacaaaata tgaaataaat tatataaagt
     8641 cattcccatg acaaagaata acatcccttt aaaacagtat cttacccaat tttcaggtaa
     8701 gacttttgat acaaccgtcc ccaatacccc tccgcatata gaagcaagaa aaataactgg
     8761 aacaggtgct aaatgatact gatttaaacc tgtaaccaca acaattgtag ccaaatgaga
     8821 cagctgggaa aagaaaataa ttgcaagaga atacatcgtt gcttctttta tcgaaataga
     8881 aaaaaacacc atcaataaag atacattgag cggaccacca ccaatcccta aaaaactaga
     8941 aattaagccc aatagaagac cacaaatcat atagaatcct atcttatcaa attgataagt
     9001 ttttttaaaa tctgttaaaa caaatgatac aagtagcatc acaaatagta aaatcatttg
     9061 aacaatggat actagccgta ctgataactg agataacaaa acttgaaaaa taagatgacc
     9121 tagaaatcct cctaaaacag aagaaaagga taccgtaaaa atcaatctcc attttatctc
     9181 tttactttgt gaaaatcttt tgattgttga aataatcgcc attataaaaa cagagaaact
     9241 agaataaaaa gcaatatcac tgaccgaatg gtatccaaaa ctatccataa gtggcttaat
     9301 tataattcca ccacccatgc ctgatataga acccaatcca ctagctatta taatgattag
     9361 aaaataaata atctcaacca ctttttaagt ccatcctatt ctttcaaaca tctaaaacct
     9421 agatggctac tagtggaagt agagatacat ccatttctag cagctactcg gtatcgatta
     9481 caataagaac aatgacataa aaaggaacct cctctgatag catactcctc atcttgtatc
     9541 ccatattttg gcaactcata gtcatcttca ttaaaactag ctaataaggt atatctcgga
     9601 ttgcgacacc attcccaaac attcccaatc atttgatata gaccaaaatc attaggtaaa
     9661 aattcataga caggagccgt tcctataaat ctatctaagg cagtattttc ataaggaaat
     9721 cttccttgcc aagtattggc atgataatat ccaccttcta aaagagtatc tccccaagga
     9781 tattcagatt gtcttcctcc tctagcagca tattcccact gtgcttcagt aggtaaagac
     9841 attccgctcc aattacagaa agccaatgca tcttctaaag caacatgaac tactggatgg
     9901 tcttctaaac ccactagatt actgttctca ccgtagggat gtttccagca agcgttcgga
     9961 acttgtaacc accacggagc cccagcagga tgaggatatc cctccctttc tgcctctgga
    10021 acgaacaata taaaaacaaa agaccactct tgtcgctcag ctagtgtttt atagcctgtt
    10081 tctttcacaa attgagcaaa ttcttgattc gtaactggag tatccgcaat ggaaaaatcc
    10141 tttacactaa cagcgacttg aggaccttca aaatcatcta aaaagccctc ttcagaattt
    10201 gttcccatca taaatgtccc acccttgatt tttttcattt tcatgattga ctatcccccg
    10261 ttttcgttag agttggcgtt attttagaaa gttcaacagg aactaactct tcatccacaa
    10321 caaaaccttc ttctctatat ctaagaaagt ctactaattt tttcttcatc tgacgaacaa
    10381 taggttggta tttttcgctt ggatacaaat cattcatttc atgcggatct tttttcatat
    10441 caaataactg ataatgattt aaaacaggaa accagataaa tttccactgg tctgtcaaga
    10501 tatattgact agaatctttt cctaaagcat gttcaccatg aaattctgtt cgccacccct
    10561 cgtactgtcc aaataataaa ttcttaacac tccgtccatc tagttcatct gtagtagttc
    10621 ctcctgccaa atccacgagt gaaggaaaaa tatcttgaat tttcactaat tgcttgattg
    10681 ttcccctatt accagctatc aatccagccg gatcgtaaat aaacgaagga atgtggatac
    10741 ttccctgata aggatatccc tttctaaaca gataatgttc accaagctga tctccatgat
    10801 ccgaaacaaa ccagataatc gtatctttat catgacgaaa ttcttttagg gaagtcaaaa
    10861 agcgactaat ttgatggtct atatgagtaa tcaatccaaa ataagcagca accattcttc
    10921 tttgatcatc ttcctttaat ttccctctta aagcataaat agacggaatt tgcttttcta
    10981 atacttccca attcccgata tgcaaatcga gaaattgagg caagcgttcc atatacatat
    11041 caaaatagta ttttggggga tttaatggag catgtggttt ttcaaaagac attttgagaa
    11101 aaaaaggaac cgttggatct cttctttgta aaaatgaaat ggactcactc acaacccagt
    11161 ttgttggatg taacttctca tctttatccc aaggtctagc ttcccaagaa ttacaatcca
    11221 ttccgtcatc aatcaaatca acatcataac caactttacc ctttaaaaag gctagatagt
    11281 cactaacata atcaaattga cttccataag ccttatcata ctttctatca acatgtagat
    11341 acccatcgtg aagtaagaca tgatcaaacc caagtctctg tctggaagga aaaacatgca
    11401 tcttaccaat acactctgtt tgataaccca tatctttaaa gacttttgga agagtattag
    11461 taaaattcca aggcacctca tcttgataac caacacgtcc acttttatct tggtctaagc
    11521 cagtcaataa agctgcccta gcaggaacgc aagaaggaac tggagaatag gcattttcaa
    11581 aattataacc tacactagcc atcatatcta atgttggagt gctaactagc ttatccttac
    11641 tattcaagga taaggcgtct gctctcattt gatctacaac aatcaaaata atatttggct
    11701 gttttgtctg aaccataaaa tctcctttct aatatggcaa aagaggcaca agaagatatc
    11761 tacctttact gcaccccttt ctatatcaat ctctctatat aaagcaataa cattcttgtt
    11821 atgttttata gaacaatgaa ctaaaatatg actaaatcga ttaggaaatt caaatcattt
    11881 tctagtactg ttttagaagt tacagtgtac tattccaact tcaacaaact ataaaccttg
    11941 gtctaataac aattttagtg gagataagaa atcctacacc taactcatct tacacgtaat
    12001 ctatttctat tttatcacaa aaaacgcaag taagaccatt aactcaattc agttttatct
    12061 gccattttca caaatgggaa ataagtcaag acactaataa tcaaacaaac aactgataag
    12121 atgatggcac gccaatcaaa tgctgtagag aagaaaccat ataaaattgg aggcattacc
    12181 caagtaacat tttgtgtaac aggtgaaaca agaccccagc ttgttgccca gtaagctacc
    12241 gttgccatga aaaccgggct aagtacaaat ggtataaata gcaaaggatt caagacaact
    12301 ggtaaaccat aattgaccgg ctcaccaata ttaaacagaa ctggtgctag accaagttta
    12361 gcaacttttc gataatgact gtttcttgaa aaaattaaaa tagcaagtac caatcctaat
    12421 cctccaaacc agacaaacgc cccaaaagac ccacttgtcc atatataagg aatcggttca
    12481 cctttttgga aagcatccag attcgctaac atagcaactc caaacagccc ttccatgatg
    12541 ggagccaata catttcctcc atggagacca aaaaaccaga ataacttatt taaaaagatc
    12601 atcagaataa ctgcaaagaa actttgagac aaacctagta atggcgtttg taacaccttg
    12661 taaacccaat caatcaataa gtcattgcta agtaaatgga aaacataagt caagatggct
    12721 actatataca tcgccataaa tcctggaatg atagaagtga acggcttagc aatcgcaggg
    12781 ggaactgaat ctggtaactt gattacccag ttctttttca ttactttaca gaaaataata
    12841 gaggctaaaa atccaatcat catggctgta aagtagcctc tggcattaat atggtttcct
    12901 ggaatcacat tcccaatagt taccatcaga tttttaccat caaatgctag attatcaatt
    12961 ccttgtaaga tctgatctaa tttcacatct cctacatttg ccaaagggaa actctttgta
    13021 actgtacttc caatcgaaat gacaaacgaa gcaagtgata ccaaaccagc agaaactgta
    13081 tcaaccttgt aaatcttagc gatattcact cccaagcaat agatgaacaa caaagaaaca
    13141 attggtatac ttcccttgaa taccaaatta ttgatgtcaa caagccactg aaaggttttc
    13201 gtaatacttt ctaggtgaaa ttgttgtggt aaatccacta gaaaagcatt taataacaaa
    13261 gcaatggaac ctgtcataat aacaggcata gtccccacaa atgaatcacg taaagcaact
    13321 aaaaacttct gattcccaac tttcattgca attggagaaa gtttttcttg cattttatcc
    13381 aacattgtat tcatactaaa ctcctaattt ttttcaaatc gttcgtatac ttctaccact
    13441 tctttcgcca agtcaacaaa agtcagactc gtcattaaat gatcttgcgc gtgtaccatc
    13501 aagaggctaa ttgatgtttc ttccccctgt gcctctcttg tcaacatttc tgtttgagcc
    13561 ttatgcgcct gtaataaggc agcattcgca tcagctaatc ttcgattggc tttagagaaa
    13621 tccccttttt ttgctgcctg aatagcctcc atagcattac ttttggcttc cccaccatac
    13681 ataattagcc ccattacaga ttctaaatta ctttcatcca taacattctc ctcaatctta
    13741 gtctaataaa ctttcagcta aatctaaaac tttagaacca ttcatcattc cgtaatctgt
    13801 catcggaata accgccacag gaatctgtct atcttttagt ttttcttgaa agtcccctag
    13861 taaatagcga acttgggggc ctaaaagcaa cacatttact tcttttgttg ctacaatttc
    13921 ttctgcttca ggagctggaa ctgcaaaaat ttctgcatcc aaccccttat cttctgctgc
    13981 cttttgcatc tttgtcacta gcagacttgt actcatacct gctgcacacg ctaacataat
    14041 tgttacttta gccatagctt actcctttta ttttattgtc aatgttactg tttttcgatc
    14101 aactttagac tcataatcta aatgtttggg acgactagta taatgcaaat tataaactcc
    14161 tggaggcatt tgtgaaatct tttctttttc agttttattg cgaattaata aatctaccga
    14221 agtattccct ccaaagttat taaacatagg ttcattttca atagaataat ctaactcata
    14281 tccactatta gttgtaattc cttttaataa atcaggaaca tctcctctgt ttattactat
    14341 atccttagcg ccatttattt tgggagattg actataatac tcttcagctt gatgttgttg
    14401 taaatcattt ctagagtaag aaacataatc aactacaaat tcagtccctg ataaattatt
    14461 gtccactacc ccaggccaac ttcctcccat cgctaaattt aactgaacat actgaggtct
    14521 attaaaaatc tttttcgcta tattatctga tccataatat acttttctaa caatatgatt
    14581 gtcaaccatc catacaatat aatctttata ccaatcaatt gaaaaagtat gatacgaatc
    14641 attaaagttt ccactcgata atttaaaggc tgtaccacgt cctgcaaatt ttccatcatc
    14701 attatccgac tgtccataat gaagcgtttg ataaactgtc ttattcccac ttccatttga
    14761 atttcgttca ccaactaatt ccattatatc tatctcacct gttgacggcc atcctcgtcc
    14821 ctgaacagga ttaatcttcc catcaagtgt aaaatccgat ccaagagtcc agaaagcagg
    14881 aaaaactcct tgacctttcg gtaattttgc acgcatctct aatttaccat aaagaaattc
    14941 tactttccca tgtgttctaa cacttcctga attatataca acctttctgt gattgtttct
    15001 tgggttaaaa tactgatctt ccttagagcg attcgtagct ctaagaacta aatttcccga
    15061 acgtagaaaa acattctcat cacttcttac ataatgttgc tgttcccaac cacgaattga
    15121 tcctaattca tatccccaat attttttatt taattgttcg ccatcaaaat catctctcca
    15181 aattttctta tatgaatttc ctttagacag ttgatacatc tctacattat caataaaaat
    15241 attagatttt tttgtagtat cgtcttctga ccatttcact aattgaatca aaactttatc
    15301 gtgtttaccg gaattgaaag aaaacatgta ttttttccat tcggttgtat taactgtttg
    15361 atcaacagct tctccaatgt tattttgaac aatctctcct tgcttattat tatcaaacca
    15421 tcttgcggta aaatatccct ctgctccact aatatctact ttggcaaaag cagatactaa
    15481 ataatcttca ttaggttcta tatcaataac ttgtccaata tatccatctg ctctattatc
    15541 attaattgca gtagaaggaa tcatacctga gtaatgtccg ttactataac tctcatttga
    15601 attaattatt cccctatttc tagagttcca aaacagctct ccttgctcaa aatttgaatt
    15661 ttttattaaa ttttcattag caaaaacttt cacatgaata aatattaata aaaataatat
    15721 aaataaactt gaaattaatt tcttattctt cattttatca cctatttact gacctagcca
    15781 agcgagatac tgtatgtctc actcctctaa ttgccttgct caatgcataa atattatcga
    15841 ctgttgccag tcccccatat cctgcgtcac ctatatgttg aatatcaact ccacaaattt
    15901 tatttctaag tgcaatttcc ttgatagtat ctgtatcaga tgtttcttgg ctagtaccaa
    15961 tagcactcag tactagccca cccttactat gaacgagatc aacgacttca cgcaactctt
    16021 ggtcatgaaa ggctggaacg gttccaactg ctggaacaag tatcacatcc gcacctgctt
    16081 ccaacaattg ctctgcgaca gaaagctctg ccacaggctc attcactcct gccccgtgca
    16141 tcttacctgc aataatcaaa ccagaaaagt tttccttagc agtttgaaca gccttaatga
    16201 tttctcgatt gctaactcct gttccaggat ttccagtcaa acagacaaag tcaaaaccta
    16261 actcctctat tcgattcaat gtttcaacac tagcaacacg acctgcaaca atttcctgtg
    16321 tttcctctag catctttgca gatgggtcaa tcggttccaa atttacacca attggacaag
    16381 caacaagctg gtgtaagcga tgaataacat catcacctga actatccaaa gcataaattt
    16441 taggctcaaa aacatcaaca caattcaata aaatcatatc agctccaaaa gctctagcaa
    16501 tttcagaatt agttatatcc ccaacaaatg tctcacgagt aactacattt tcagatagaa
    16561 caactctacc ttcacttgct aaaatacttt gttttaattc ttcagcattc atctgtaata
    16621 tctcagatgg atttgcacta attaaacgtt tcattctcaa tcctccaata acttaaatcg
    16681 tttttcaata gctattacaa atggtaaata aagtaagata tccaaaacaa tcaatacaat
    16741 ttgtagtaat gaaccagaaa tacttcctgt agcaagtaaa ccacttataa caggtggcat
    16801 agtccaacct ggatctgtat aagttagcgg aaccaaacct gatgccattg ctccccacgc
    16861 tactagtaag ttaaacattg gagctaagat aaatggaact agtaacaaaa tgttcaaaac
    16921 aataggaacg ccaaacattg ctggttcatt tatattaaat aatccaggta taacagttat
    16981 aggtgccaaa gtttttagtt gtttactagc tttcttctta tgtgctaaat atcctaacgc
    17041 aagaactaaa cctatggttg caccaccgcc acctatgaat acaaaattat ccataaatac
    17101 gctagtaaaa atatttggaa gagtttctcc agtttgataa gctactttat ttgcatctag
    17161 attagctaac cataacggtt tcataactgc attaacaaca tttcctccgt gtaatcctac
    17221 aaaccaaaaa ctactattaa ggaaaattaa gataagaaga ccaataacat tatttcctaa
    17281 aagtccaagt ggacctccca aagctacttg agcaatttca tgaagattag gtaaacttaa
    17341 tttatctaaa gttgcataaa caatcaacca accaacaata gtcactgcac caggaataat
    17401 ggcactaaag cttttagata ctgctggtgg aacaccatcc ggcattttta tctgtatatt
    17461 atgatttata aaccattggt agatatagcc attaatcaat cctaaaatca tagcaacaaa
    17521 taaacctttt gatgccatga aagcggtagg cattcctgct ccagcctctc ctctaatgaa
    17581 aggcgtaact gtaataaaag atgataatga cacaatacca gcaggaatac catcaacttt
    17641 gtattgtcta gccataaagt aagcgatacc aaaactagct acaagaccaa gtaatccaaa
    17701 actactatca acacccttcc ataaatagtc agcaacacca atatcaccca gatatttttc
    17761 ccatcctgga ataggaaaac ttgcaataac cattagtaat gaacctatca acaataaagg
    17821 tatagttaga gtaataccgt ctcgaataga aaccaacgct ttattattac ccaatctagc
    17881 cgctactgga agtaactttt cttgtaataa tttttccata accttttcct cttaaatata
    17941 ttttattatc aaaagttatc aattatcttt attttttgat gtcaagttgt tatttgatta
    18001 tcttttgtta tttatatttt acaactataa gaaagccttg tcaaggattt gtgtaaacat
    18061 ttgattattt tttattatca atatataata aaaagagacc agtaagtaat gaaaagctta
    18121 aaaagattaa catctacatt tatccatagc aaaaaatcat aggattttaa gaatctgaca
    18181 actaggaaga aaactaatat gaccttatct aagaaacaat tacaactaag agctaaaata
    18241 ctggaaacag tttacacatt aggtcccatt tcacgaattg aaatcgctac taaaacagga
    18301 ataacacctg caacaactag cagtatcaca aatgatctga ttaaagaaaa tattctctta
    18361 gaacttggtg aagacgaaca cgatacaagc gtagggagaa aaaaaatcct gctagacatt
    18421 caagctaaac gattttacta cattggttgt gaactatctg aaaaacattt cacttttgct
    18481 ttaggagata atctaggaaa tatcttaaag gaagagaaag aaattgttac taaacagtta
    18541 atccaagaaa agggaaatca actcattaac cagacactaa agcaattctt aaataactgc
    18601 tctgattatg aaatagaagc aattggaatc gcgctacctg gtcgctattt agatgattat
    18661 aaaataacaa caaacaatcc tttatggcaa catatcgact tagaaatgat tcaatcccac
    18721 tttgataagc ctttgttttt ctctaacaat gttaactgta tggctatagg taaacgccta
    18781 tttagtcgcc aacagaatga ccctaatttt gcttattttc attttgctag aggtatgcac
    18841 tgttcttata tctacgacgg aaacatttat gggaaaggaa atctaatgat tggagaaata
    18901 gggcatacag ttgtttcatc tgagggagaa gaatgtagct gcgggcgcaa gggatgctta
    18961 caaacttttg ctggagaatc ttggttaatc aaaaaatcaa aaattctata tcatcaatct
    19021 ccctattctc tacttcctag tttagtcaaa aatgccgatg atattgatat ccaagttatt
    19081 ttaaccgctt atcaattagg tgacactgga ataatcacac ttatccatca agccttattc
    19141 tatcttagtc aaaccatttt aaatatcagt atgatgattg actctcaaaa aatctaccta
    19201 catagtcctt tactaacaaa tcaacacatc atccaaaaac tatatagtga aatgaactac
    19261 aaacccaaac tgctctataa tcgcctacct gaagtcatta ttgaacctta caatgatttt
    19321 acagccgcac actcagctat tgcactttgt ttgtatcata caattctaca ttcttaattt
    19381 tctattacat ttaataaata ggctaaaaag agggagtata tcataaaaag cgaataaacc
    19441 tgttttctga aaatcagaat tctgttttgt tcgctttctt tatatttgga attatactcc
    19501 tatctcccct ttttcataat gtatagtaaa atgaaataat aacaggac
//
LOCUS       DAVBCW010000028        19542 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVBCW010000028 DAVBCW010000000
VERSION     DAVBCW010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19542
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.028"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            83..1057
                     /gene="folP"
                     /locus_tag="VQ514_000867"
     CDS             83..1057
                     /gene="folP"
                     /locus_tag="VQ514_000867"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET2499646.1"
                     /translation="MMKILEGVTDMSSKANHAKTVICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALAAGANLVNDITGLMGDEKMAHVVAKAGAKVVIMFNPVMARPQHPSSLI
                     FPHFGFGQTFTEKELADFETLPIEELMEAFFERALARAAEAGIAPENILLDPGIGFGL
                     TKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEENGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            1059..2381
                     /locus_tag="VQ514_000868"
     CDS             1059..2381
                     /locus_tag="VQ514_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HET2499647.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADY
                     QSLLEGEAVANLQDTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCRPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGMPLVTGRIAPEALTVIDRIAEGKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            2362..2916
                     /gene="folE"
                     /locus_tag="VQ514_000869"
     CDS             2362..2916
                     /gene="folE"
                     /locus_tag="VQ514_000869"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HET2499648.1"
                     /translation="MDTQKIEAAVKMIIEAVGEDANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            2959..3771
                     /gene="folK"
                     /locus_tag="VQ514_000870"
     CDS             2959..3771
                     /gene="folK"
                     /locus_tag="VQ514_000870"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HET2499649.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQVNLKQAIDKLRARGIHILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(3921..4238)
                     /locus_tag="VQ514_000871"
     CDS             complement(3921..4238)
                     /locus_tag="VQ514_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HET2499650.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            4594..5040
                     /gene="rplM"
                     /locus_tag="VQ514_000872"
     CDS             4594..5040
                     /gene="rplM"
                     /locus_tag="VQ514_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HET2499651.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            5059..5451
                     /gene="rpsI"
                     /locus_tag="VQ514_000873"
     CDS             5059..5451
                     /gene="rpsI"
                     /locus_tag="VQ514_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HET2499652.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVIGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            5786..5938
                     /locus_tag="VQ514_000874"
                     /pseudo
     CDS             5786..5938
                     /locus_tag="VQ514_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6094..6432
                     /locus_tag="VQ514_000875"
     CDS             6094..6432
                     /locus_tag="VQ514_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET2499653.1"
                     /translation="MTLTIHVYYKGDGDSAIKFAREMLDSGLVEEIRNQKGNLKYEYF
                     LPIEKEGTILLIDQWINQETLDKHHQSKTMQKILDLRKKYHLQMQVERYIEDDSGMPE
                     SDKSFIDTGN"
     gene            6622..8058
                     /locus_tag="VQ514_000876"
     CDS             6622..8058
                     /locus_tag="VQ514_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HET2499654.1"
                     /translation="MTTFKDGFLWGGAVAAHQLEGGWQEGGKGISVADVMTAGRHGVA
                     REITLGVLEGKYYPNHEAIDFYHRYKEDIALFAEMGFKCFRTSIAWTRIFPKGDELEP
                     NEEGLQFYDNLFDECLKNGIEPVITLSHFEMPYHLVTEYGGWKNRKLIDFFARFAEVI
                     FKRYKDKVKYWMTFNEINNQANYQEDFAPFTNSGIVYEEGENREAIMYQAAHYELVAS
                     ARAVKIGHEINPDFQIGCMIAMCPIYPATCNPKDILMAMKAMQKRYYFADVHVLGKYP
                     EHIFKYWERKGISVDFTAQDKEDLLGGTVDYIGFSYYMSFAIDSHRENNPYFDYLETE
                     DLVKNNYVKASEWEWQIDPEGLRYALNWFTDHYHLPLFIVENGFGAIDQVAADGMVHD
                     DYRIEYLGAHIREMKKAVVEDGVDLMGYTPWGCIDLVSAGTGEMRKRYGFIYVDKDDN
                     GKGSYNRSPKKSFGWYKEVISSNGESVE"
     gene            8110..8349
                     /locus_tag="VQ514_000877"
     CDS             8110..8349
                     /locus_tag="VQ514_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family sugar transporter"
                     /protein_id="HET2499655.1"
                     /translation="MIGSIAAILTTFAFLPQVFRVVKTKDTGSIALGMYVMQVIGIAL
                     WLAHGIRIGDLPLILANSVSFLLSGIILFYKLKYK"
     gene            8479..8793
                     /locus_tag="VQ514_000878"
     CDS             8479..8793
                     /locus_tag="VQ514_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HET2499656.1"
                     /translation="MAKALIICAAGMSSSLMAQKTTEFLKGKGKDIVVDAISATEGGK
                     AIAAAAFDLYLVSPQTKMYFKQFEEAGSKAGKPVVQIPPQAYIPIPMGIEKMANLILE
                     NI"
     gene            8911..10890
                     /locus_tag="VQ514_000879"
     CDS             8911..10890
                     /locus_tag="VQ514_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HET2499657.1"
                     /translation="MNVLNIKEKDILQFLIKNKERFVTSKELAEYLSCSDRTVRNVLK
                     LIEKTMIIQGVRLISKQGQGYQIFFENQGAYQEFRQTYELEEDYTKTAVSKGDDRLVF
                     ILNKLLFEQVPVLFDDLADELYVSRSTLSHDFRKIRVMLSEYNLSIESRANKGVYVSG
                     EERDKRRFIINYFLENQFFKTIHCYVKFNFFAQTVPLEEFARIVLDECQEANLKLSDF
                     VLQNLVVHIALSVIRLKSGFEIKNIDCQMTDDAIERKVAQRILSKVREVTNQEFPVQE
                     IDYITLHLLAKSQQCQKNQKNISEEVLKKSLFKTFQNLGLDDMYNFSSDFQLIEGLIT
                     HLMTLQVRLESRITLNNPLVDEIKQNYSDIFFMTREILANMDKFLEWTISDDEVAYVS
                     LHFLAAMERSKESTKFNILAICATGFGAAQMLRNRLETEFGKRVEVVDVIGYYELNQE
                     KLKGIDFIVSAVDLSNLYFQIPVFKVSVFLKSDEMEMIRKAMDQMQVSSYVQSSKINK
                     FENNSFRQYFSKENFLICTESDKVNLLEKMVESLSVGESNEFEQSLLYGIKQREELSS
                     VVFSEKIAVPHPIQPFGTEGKVSVAICKDSLLWDNQSSYVQLVFLLSPSIYGNEGLAT
                     VTKKIVSLTENDELQNQLISCNNFEDFINIFEKIK"
     gene            10900..11214
                     /locus_tag="VQ514_000880"
     CDS             10900..11214
                     /locus_tag="VQ514_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HET2499658.1"
                     /translation="MNQEELQVAAFEIILHSGNARSEIHEAFAKMREGSFDDAESKLN
                     QSNEIILEAHHAQTKLLQEYASGVEIKIEIIMVHAQDHLMTTMTLLEVAKEMLALYKK
                     VN"
     gene            11245..11751
                     /locus_tag="VQ514_000881"
     CDS             11245..11751
                     /locus_tag="VQ514_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499659.1"
                     /translation="MDKKERQKIEQQRREMALTNTFFNRYLLLRYSIALFFFGNIYWL
                     LNQFISPSPIIIFPIMLIVFSILATIEQFKLYGNRKEKLGITLMFVRIQMLISIGLLV
                     LTWTSWFENLFPIFENNQVARLFVFVVLLLGLVLSLLDIRRIKKIYKRTDKAYQQFVQ
                     LEKNSLSL"
     gene            11831..13177
                     /gene="celB"
                     /locus_tag="VQ514_000882"
     CDS             11831..13177
                     /gene="celB"
                     /locus_tag="VQ514_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HET2499660.1"
                     /translation="MDQQNGLFGFLENHVMGPMGKLAQFKVVRAITAAGMAAVPFTIV
                     GSMFLVFSILPQAFSFWPIVADIFSASFDKFTSLYMVANYATMGSLSLYFVLSLAYEL
                     TKIYAEEEELNMNSLNGALLALMAFVMTVPQIIFDGGMMKTVTSLKEGAVIADGWAMG
                     NGVARFGTTGIFTAIIMAIVTVLIYRMCVKHNWVIKMPEAVPEGVSRGFTALVPGFVV
                     AFVVIFINGLLVAMGTDIFKVIAIPFGFVSNLTNSWIGLMIIYLLTQLLWIVGIHGAN
                     IVFAFVSPIALANMAENAAGGHFAVAGEFSNMFVIAGGSGATLGLCLYIAFASKSEQL
                     KAIGRASVVPALFNINEPLIFGLPIIYNPALAIPFILAPMVTATIYYVANSLNFIKPI
                     IAQVPWPTPVGIGAFLGTADLRAVLVALVCAFAAFLVYLPFIRVYDQKLVKEEQGI"
     gene            13557..13733
                     /locus_tag="VQ514_000883"
     CDS             13557..13733
                     /locus_tag="VQ514_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499661.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALSLGMT"
     gene            14135..16348
                     /locus_tag="VQ514_000884"
     CDS             14135..16348
                     /locus_tag="VQ514_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HET2499662.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSRTGNFEDRTTQLIYNRDFGQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEDEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(16570..17046)
                     /locus_tag="VQ514_000885"
     CDS             complement(16570..17046)
                     /locus_tag="VQ514_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HET2499663.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDHKSGPLGKRIEWNFAKFLIGRDGQVFERFSSKTNPKQIEEAIQTLL"
     gene            17269..>19542
                     /locus_tag="VQ514_000886"
     CDS             17269..>19542
                     /locus_tag="VQ514_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HET2499664.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSTWVDQKNSSADASTRVIEAKDG
                     AITISSHEKLRAALHRMVPIEAKKKYKLRFKIKTDHKVGIAKVRIIEESGKDKRLWNS
                     ATTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVADQPSEDSQT
                     DKQLEEKIDLPIGKKHVFSLADYTYKVENPDIASVKNGILEPLKEGTTNVIVSKDGKE
                     VKKIPLKILASVKDAYTDRLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSI
                     SSQADRTYLWEKFSNYKMSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSME
                     WMHKHVYNSEKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDP
                     EHFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGF
                     YQDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKNPIDKDKMQTMYHWIDKSFAP
                     LLVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQ
                     SDSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRTSYLSAFNKMDKTAMYNAEKGFGF
                     GLSLFSSRTLNYEHMNKENKSGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTE
                     TDAKRADSDTGKVLPSAF"
BASE COUNT         6261 a         3080 c         4187 g         6014 t
ORIGIN      
        1 aggaaccgac ctctttctac tagagaaaga tgaatgtaat cgtgtccatc tttttctttt
       61 tatggtaaaa tagaaaaata atatgatgaa aatccttgag ggagtgaccg atatgtcaag
      121 taaagccaat catgcaaaga cagttatttg cggaattatc aatgtaaccc cagactcctt
      181 ttcggacggt ggtcaatttt ttgctcttga gcaggcactc cagcaggctc gtaaattgat
      241 agcagaagga gccagtatgc tcgatatcgg cggagaatcg actcggccgg gcagtagcta
      301 tgttgagata gaagaggaaa tccagcgtgt tgttccagtg atcaaagcga ttcgcaagga
      361 aagtgatgtc cttatttcta tcgatacttg gaaaagtcag gtggcagagg ctgctttggc
      421 tgcgggtgcc aatctagtta atgatatcac tggtctcatg ggtgatgaaa aaatggccca
      481 tgtggttgct aaagctggag cgaaagtggt catcatgttt aatccagtta tggcgcgacc
      541 tcagcatcct agttcgctta tcttccctca ttttggcttt ggtcaaacct ttacagaaaa
      601 agagttagct gactttgaaa cattgccaat tgaagaattg atggaggctt tctttgaacg
      661 agcactagcg agagcggcag aagctggtat tgcaccagaa aatatcctgt tggatccagg
      721 aattggcttt ggtctgacca agaaagaaaa tctgcttctt ttacgggacc tggataaact
      781 acatcagaag ggctatccaa tctttctcgg agtgtcgcgc aagcgatttg tcatcaatat
      841 tctagaggag aatggttttg aagtcaatcc tgagacagag cttggtttcc gaaatcggga
      901 cacggcttcg gctcatgtaa ctagtatcgc tgcgagacag ggtgtagaag tggtgcgcgt
      961 gcatgacgta gctagtcaca ggatggcagt tgaaattgcc tctgccattc gtctggctga
     1021 tgaagcggaa aatttagatt taaaacagta taaataagat gaaagaaatt gaaaacaatc
     1081 agtggattgc taactaccgg acggatcaac cgcattttgg cttggaacga atggtggaac
     1141 tgttagcttt gcgtggcaat ccccatctca aactcaaggt tctccatatt ggagggacta
     1201 acggcaaggg ctcgactatt gcttttttga aaaagatgct agaaaagcta gggctgagag
     1261 ttggcgtgtt tagttcgccc tatctcattc attacacaga ccagattagc atcaatgggg
     1321 aatcgatctc agaagcgagg ctagaagctc tcatggcaga ctatcagtct ttgctggagg
     1381 gagaagcggt cgccaattta caggacacaa ccgagtttga gattatcaca gccctggcct
     1441 atgactactt tgcctcagag caagtagatg tggccatcat ggaagttggc atgggcggac
     1501 ttttggatag taccaatgtc tgtcggccca ttttgacagg aattacaact attggcttgg
     1561 atcatgtggc cctacttggt gacaccttgg aggccatagc agagcagaag gcaggtatta
     1621 tcaaacaagg gatgcccttg gtaacagggc gtattgctcc agaagccttg actgtgattg
     1681 accgcattgc ggaagggaaa gatgcgccga gacttgccta cgggacagat tatcaggttc
     1741 gtcatcaaga aagtgtggtg acaggggaag tctttgacta tacaagtgct gtcagacaag
     1801 gtcgcttcca gactagcctg cttggtttgt accaaataga gaatgctggg atggccatag
     1861 ctttacttga tactttttgt caagaagatg gtcgagagct agcaagcaat gactttcttg
     1921 gtcaagcctt ggaagaaaca agttggccag ggcgtttgga aatcgtgtca agagatccct
     1981 tgatgatttt ggatggagcc cataatcccc atgctatcaa ggccttgttg gtaaccttgc
     2041 aagaacgttt tgcggattat cataaggaaa tcctcttcac ttgtatcaaa accaaagcct
     2101 tggaggatat gttggacttg ctgggagcca tgccagatac cgagcttact ctaacacatt
     2161 ttgcggatag tcgggcgacg gatgaaagcg tgctgaaaga ggcagctaag tctagaaatc
     2221 tcagctacca agattggcat gattttctag agcagaattt gacagataaa aaagaagaga
     2281 aacaaacagt taggattgtc acaggttcct tgtatttctt gagccaagtg agggcctatc
     2341 tgatggagag gaagaacgag aatggataca caaaagattg aagcagctgt aaaaatgatt
     2401 atcgaggctg taggagagga cgctaatcgc gagggcttgc aggaaacacc tgctcgtgta
     2461 gcccgtatgt atcaagagat tttttcaggt cttggtcaaa cagcagagga acatttgtca
     2521 aaatcctttg aaattattga cgataatatg gtggtagaaa aggatatctt tttccatacc
     2581 atgtgtgaac accacttctt gccattttat ggtagagcgc acattgccta cattccagat
     2641 ggtcgtgtgg caggcttgtc taagctagcc cgtacggttg aagtttattc gaaaaaacca
     2701 caaattcaag aacgtttgaa tatcgaagtg gccgatgcct tgatggacta tctaggtgct
     2761 aaaggagcct ttgttgtcat tgaggcggaa catatgtgta tgagtatgcg tggtgttaga
     2821 aaaccaggca ctgcaacctt gacgacagta gctcgtggtc tatttgaaac agataaggac
     2881 ctccgcgacc aagcttatcg tttaatgggg ctataaaaag aatccgcttc aagcggattt
     2941 ttctagaaag gaatcattat ggatcaactg cagattaagg atttggaaat gtttgcctat
     3001 catggtcttt ttcctagtga gaaagaattg gggcagaaat ttgtcgtttc agccatccta
     3061 tcctatgata tgaccaaggc agctacagac ttggatttaa cagcctctgt ccattacgga
     3121 gaattgtgtc agcagtggac gacttggttt caggaaacga gtgaagattt gattgaaacg
     3181 gtagcctata aactggtgga acgtaccttt gagtcttatc ctcttgtcca agaaatgaag
     3241 ttggaactga aaaaaccttg ggcaccggtg catttgtcac tagatacttg ctcggtaacc
     3301 attcatcgcc gcaagcaacg agcctttatc gccctaggaa gcaatatggg agataaacaa
     3361 gtaaacttga agcaagccat tgacaaactg cgagctcgtg gcatccatat tctcaaagag
     3421 tccagtgtct tagcgacgga gccttggggt ggagtggagc aggatagctt tgccaatcaa
     3481 gtggttgagg tggaaacctg gctaccagca caagacttgt tagaaacctt gttagccatt
     3541 gagtcagagc tgggacgggt gagagaagtg cattggggac ctcgtttgat tgatttggac
     3601 ttgctctttg tggaggacca gatcctttat acagacgacc tcatattgcc tcatccttac
     3661 atagcggaac gcctttttgt ccttgagtcc ttacaggaaa ttgcgcctca ttttatccat
     3721 ccgatattaa aacaaccgat ccgcaacttg tatgatgctt tgaaaaaata gaaaaactct
     3781 agttttcagt tacttgcaac tgaaggctag agtttttata ctcttcgaaa atctcttcaa
     3841 accacgtcag cgtcgcctta ccgtactcaa gtacagcttg cggctagctt cctagtttgc
     3901 tctttgattt tcattgagta ttaaaatagg tcattttctt ctgggaggag gatagtttct
     3961 ctaccgtcca tgtctaaaac cagtactctt gggggataac gagggtcgaa aggatggtta
     4021 aagtcaaaat caatggctgt agggaggtgt tgacttgaaa agtggaaggt aatctttcct
     4081 tggttattaa gcaattgaaa ctcgagttct tcttccaatt caaagacatt ttttaagaaa
     4141 tggtcgatga tataccaaaa agagtcaatg atgtcatcag gcaagctggt aacaatgcca
     4201 aaactagcag atcgcatgtg ggtattggta aaagccatat ctctgtcccc tttcttttcc
     4261 cttatcatac agcaaatagg attaaaaatc aagaaaaggt gattttttga aaaggatttt
     4321 agttacaggg agaaataggg aaaaaattcc taaaaatcta ccgaagttaa taggtaaatt
     4381 cccaaattaa cttgattata taactttcag ttactttgag aagttaccga aaaatatttt
     4441 tcatatctat tgacttttag gggtaaaatt tggtatgata gtaggcggta ttgtttaccc
     4501 catttgaaag gccccggaac cttccaaata cttttcgatg ggaaggaaca cccatcaccg
     4561 taaacaaaaa tcgaactata tataggagaa atcatgaaca aaacaacatt tatggctaaa
     4621 ccaggccaag ttgaacgtaa atggtacgta gttgacgcaa ctgatgtacc acttggacgt
     4681 ctttctgcag tagttgctag cgtacttcgc ggaaaaaaca aaccaacatt tacaccacac
     4741 actgatacag gtgacttcgt gattgttatc aatgctgaaa aagttaaatt gactggtaaa
     4801 aaagcaactg ataaaatcta ctacactcac tcaaaccacc caggtggatt gaaacaaatc
     4861 tctgcaggtg aacttcgttc taaaaatgca gtacgtttga ttgagaaatc agttaaaggt
     4921 atgcttccac acaatacact tggacgcgct caaggtatga agttgaaagt atttgttgga
     4981 gctgagcaca ctcacgctgc acaacaacca gaagttcttg acatttcagg acttatctaa
     5041 ggaaaggaac aataaagtat gtcacaagca caatatgcag gtactggacg tcgtaaaaac
     5101 gctgttgcac gcgttcgcct tgttccagga actggtaaaa tcactgttaa caaaaaagat
     5161 gttgaagagt acatcccaca cgctgacctt cgtcttgtca tcaaccaacc attcgcagtt
     5221 acttcaactg taggttcata cgacgttttc gttaacgtta taggtggtgg atacgctggt
     5281 caatcaggag ctatccgtca cggtatcgct cgtgcccttc ttcaagtaga cccagacttc
     5341 cgcgattcat tgaaacgcgc aggacttctt acacgtgact cacgtaaagt tgaacgtaag
     5401 aaaccaggtc ttaagaaagc tcgtaaagca tcacaattta gtaaacgtta attcgaaaga
     5461 attactatac ttatacagag cacctttcgg ggtgttcttt ttttatactt tcttactaaa
     5521 ttggtgcaat tgacacagtt gttgcgactt tagtcgctta caatgacatg gtcagttgcc
     5581 tcaaaacgtt aatcaatacg attatatcaa cgtttcaaag cactcaaaag tttaccttat
     5641 gggtgctttt ttcgtgtttt tttgaaaagt ttaccttatt tttatagtaa ggtgaaataa
     5701 gaataggaca aaccgaggat agtcaaatcg aattctaaca atgttttaga agtagaggtg
     5761 tactattcta gtttcaatct actatattat ggataatgca agattccata gaatggggaa
     5821 gctagaactc ttgtgtgaag agtttaggcg taaactttta cctcttcctc cctactcacc
     5881 tgagtacaat cctattgaga aaacatgggc tcttttgtcc tgctcttgtt tcaattgact
     5941 ataaatcgct gtaagaaaaa atcattttat tggtataaag aagtgattga aagtaatggc
     6001 gaaacgttag ataattagtt cataaaaaac aagtttagct cctcatctaa acttgttttt
     6061 ttaaaattga aggatgattt tggaggatat aagatgacat tgactattca tgtatattat
     6121 aaaggtgatg gagatagtgc aataaagttt gcaagagaaa tgcttgatag tggccttgtt
     6181 gaagaaatta gaaatcaaaa aggtaacttg aaatacgaat attttttacc aattgaaaaa
     6241 gagggaacta ttctccttat tgatcagtgg ataaatcaag aaactttaga taagcatcat
     6301 caatcaaaga caatgcagaa aatcttagat ttaagaaaaa aatatcactt gcagatgcaa
     6361 gttgaacgtt atatagaaga tgattctggg atgccagaaa gcgataagtc gtttatagac
     6421 acagggaatt agaattattg tgagagtttt tgtagtttga aagtattgaa aatcgtaaag
     6481 atatcattac gagaatacaa gaagatattg ttctatttcc attaaaaatt acttctttcc
     6541 ttaacaataa ggaaaaagaa tgcgttttca tcttttggtt tgaggtttat aataagagta
     6601 tcaaagtcag gaggaaaaaa gatgactact tttaaagatg gatttttatg gggtggtgct
     6661 gttgctgctc atcaacttga aggtggatgg caagaaggtg gcaagggaat tagtgttgct
     6721 gatgttatga ctgctggtcg tcatggagta gctcgtgaaa ttactttggg agttttagag
     6781 ggtaaatatt atccaaatca tgaggcgata gatttttatc atcgttataa agaagacata
     6841 gcactttttg ctgaaatggg attcaagtgc ttccgtacct ctattgcatg gacacgtatc
     6901 tttccaaaag gtgatgagtt agagccgaat gaagaaggat tacagtttta tgataatctt
     6961 tttgatgaat gcttaaagaa tggtattgaa cctgttatca ctctatctca ttttgaaatg
     7021 ccttatcact tagtgaccga atatggtggt tggaaaaata ggaaattgat tgatttcttt
     7081 gctcgttttg cagaagtcat atttaagcgt tacaaagata aggttaaata ttggatgact
     7141 ttcaatgaaa tcaataatca agcgaattat caggaagatt ttgcaccatt tactaactca
     7201 ggtattgtat atgaggaagg tgagaataga gaggcaatta tgtatcaagc agcacattac
     7261 gaattagttg cttctgcacg agctgtaaaa attggtcatg agattaatcc agattttcaa
     7321 ataggttgta tgattgcgat gtgtccaatt tatccagcta cttgcaatcc taaggatatc
     7381 ttaatggcaa tgaaagctat gcaaaagcgt tattattttg ctgatgtgca tgttttaggt
     7441 aaatatcctg agcatatttt caagtattgg gaacgaaaag gtatttcagt tgattttact
     7501 gcccaggata aagaagattt acttggtggg actgtagatt acattggttt cagttactat
     7561 atgtcctttg ctatcgactc tcatcgtgaa aataatcctt attttgatta tcttgaaaca
     7621 gaagatttag tgaaaaataa ttatgttaag gcttctgaat gggagtggca aattgatcca
     7681 gaaggtttgc gttatgcgtt aaattggttt acagaccact atcacttacc actctttatt
     7741 gttgaaaatg gttttggagc tatagatcaa gttgcagcag atggtatggt acatgatgat
     7801 tatagaattg aatatctagg tgcccatatt cgtgaaatga aaaaggctgt agttgaagat
     7861 ggtgttgatt taatgggtta tactccatgg ggatgtattg atttggtttc agctggtacc
     7921 ggtgaaatgc ggaaacgtta tggctttatt tatgtagata aagatgataa tgggaaggga
     7981 agttataatc gttccccgaa aaaatctttt ggctggtata aggaagttat ttcatctaac
     8041 ggtgaatcag tagaatagta taaacaaact gagattattc tcagtttgtt tagcatattg
     8101 aggtgacata tgattggaag tattgcagca attttaacaa catttgcatt tttaccacaa
     8161 gtttttcgtg tagttaagac aaaggacaca ggatctattg ctctaggtat gtatgttatg
     8221 caagttattg gtattgcatt gtggcttgct catggtattc gtataggaga cttgcctttg
     8281 attttagcaa atagtgtttc atttctactc tcaggaataa ttttatttta taaattgaag
     8341 tataaataat ttttttccat atcatttagg aaaatgaggg aattgtcatt ggaaacgctt
     8401 tattttataa taaaaataga taacaaataa gccaacttag cacagctttg attggattta
     8461 agaaaaggag aaaatattat ggcaaaagct ttaattattt gtgcagcagg aatgtcttca
     8521 tcacttatgg cacagaaaac aacagagttt ctaaaaggaa aagggaaaga tattgtagtt
     8581 gatgctatta gtgctactga aggagggaaa gctatcgcag cagcagcttt tgatttgtat
     8641 cttgtcagcc ctcaaacaaa aatgtacttt aagcaatttg aagaggctgg ttctaaagca
     8701 ggaaaaccag ttgtacaaat acctcctcaa gcctatattc ctattccaat gggaattgaa
     8761 aaaatggcta atttgattct tgagaatatt tgatgaacac atgaaatgtc aataaatatt
     8821 taagttaaga taaattgttt tgagtttgat ataaattgat ttaagtgtat aatatgcttc
     8881 aatatatgaa aacatacgtt ttaggaggga atgaatgtgt taaacattaa agagaaagat
     8941 atccttcaat ttttaataaa aaataaggaa cgttttgtta caagcaaaga actggcagaa
     9001 tatttatcct gttcagatag aactgtccgt aatgtactga aattaattga gaagacaatg
     9061 attattcaag gagtccggtt gatttcaaaa caaggtcaag ggtatcaaat attttttgag
     9121 aatcaaggcg cttaccagga gtttagacaa acttatgaac tggaagaaga ctatacgaaa
     9181 actgcggttt caaaaggtga tgatcgttta gtatttatct taaataaatt attatttgag
     9241 caagttccag ttctttttga tgatttagct gatgaacttt acgttagtcg ttcaacatta
     9301 tctcatgatt tcagaaagat acgagtgatg ttatcagagt ataatttgtc cattgagagt
     9361 agggcgaata aaggagttta tgtctcaggc gaggaacgtg ataaacgacg ttttatcata
     9421 aattatttcc tagagaatca attttttaaa acaatacatt gctatgtaaa atttaacttt
     9481 tttgctcaaa ctgttccatt agaagaattt gctagaattg ttttggatga atgccaagag
     9541 gccaacctaa aactatctga ttttgtacta cagaatttag tagttcacat agcattgtct
     9601 gtgattcgtt taaaatccgg atttgaaata aaaaatatag actgtcagat gactgatgat
     9661 gcaatagaga gaaaggttgc tcaaagaatt ctttctaaag taagagaagt aacaaatcag
     9721 gaatttccag ttcaggaaat agactacatt actttgcatt tgttagctaa gagtcaacaa
     9781 tgtcaaaaaa atcagaagaa tatttctgaa gaagttttaa aaaaatcttt atttaaaaca
     9841 tttcagaatt taggattaga tgatatgtat aacttttctt cagatttcca actaattgaa
     9901 ggattgataa ctcatctaat gacactacaa gtacgtttag aaagccggat taccttaaat
     9961 aaccctttgg tagatgaaat taaacagaat tatagtgata tattctttat gactagagaa
    10021 attcttgcaa atatggacaa gttcttggag tggacaatat cagatgatga agtagcatat
    10081 gtaagtcttc actttttagc tgctatggaa cgtagcaaag agagtactaa gtttaatatt
    10141 cttgctattt gtgcaacagg atttggtgca gcccaaatgt taagaaatcg tttggaaacg
    10201 gagtttggga agcgagtaga agttgttgat gtaataggct attatgaatt gaatcaagaa
    10261 aaacttaaag gaatagattt tatagtgtca gctgttgatt tatcaaatct atattttcaa
    10321 attccagttt ttaaagttag tgtgtttctt aagagtgatg agatggaaat gattagaaaa
    10381 gctatggatc agatgcaagt ttctagttat gtacaatcat caaagattaa taaatttgaa
    10441 aacaacagct ttagacagta ttttagtaaa gaaaattttt taatatgtac tgaatcagac
    10501 aaggtaaatt tacttgaaaa gatggttgag agtttatctg ttggtgaatc aaatgagttt
    10561 gaacaatctt tactttatgg aattaaacag cgcgaagaat taagttcagt tgttttttct
    10621 gaaaaaattg cagttccgca tcctattcag ccctttggta cagagggaaa ggtttcggtt
    10681 gcaatttgta aagattcgtt actctgggat aatcaaagtt catatgtcca gctagtattt
    10741 cttctatctc catcaatata tgggaacgaa ggtttggcaa ctgtgactaa aaagatagtc
    10801 tctctgacag agaatgatga attgcaaaat cagctaatat cttgcaataa ctttgaagat
    10861 tttattaaca tatttgagaa gataaaatag gaggttcata tgaatcaaga ggaactgcag
    10921 gttgcagcat ttgaaattat tctgcattcg ggaaatgcaa ggtcagaaat acatgaagca
    10981 tttgcaaaaa tgagagaagg tagttttgat gatgcagaaa gtaaattaaa tcaatcaaat
    11041 gagataattc ttgaagcgca tcatgcacaa accaaactct tgcaagaata cgcaagtgga
    11101 gtggaaatta aaattgaaat tatcatggta catgctcaag accatttaat gaccactatg
    11161 acattattag aagtagcaaa ggaaatgtta gctctttata agaaagttaa ctaaatatgt
    11221 gttgttatat aaggaggaaa tgatatggat aaaaaagaac gacaaaaaat tgaacaacaa
    11281 cgtcgagaga tggctctaac taatactttt tttaatcgat atcttcttct acgttattca
    11341 atagcactct tcttttttgg gaatatttat tggcttttga atcagtttat tagtccctcg
    11401 cctattatta ttttccccat tatgctcata gtattttcta ttttagcaac tattgaacaa
    11461 tttaaacttt atggaaatag gaaagaaaag ttgggaataa cactaatgtt tgttagaata
    11521 caaatgctta tttcaatagg attacttgtt ttaacttgga caagttggtt tgagaatctt
    11581 tttccgattt ttgaaaataa ccaagtagca cgtttatttg tttttgtagt tcttctatta
    11641 ggtttagttc tgagtttgtt agacattaga agaattaaaa aaatttataa acgaacagat
    11701 aaggcttatc aacaatttgt ccaattggaa aagaactcac ttagtttata atcctgaact
    11761 agtataattt gtatttgaaa ctagtcttta aaaagtaaaa attattatgt tttattaagg
    11821 aggtctattt atggatcaac aaaacgggtt gtttggtttt cttgaaaacc atgttatggg
    11881 accaatgggc aaacttgctc agtttaaagt agtgcgtgct atcacggctg caggtatggc
    11941 tgctgtacca tttactattg taggatcaat gtttttggta ttcagtattt tgccacaagc
    12001 tttctcattt tggccaattg tggcagatat tttctctgct tcatttgata aattcacatc
    12061 actttacatg gttgcaaact atgcgactat gggttctcta tctctttatt tcgttctatc
    12121 acttgcatat gaattgacaa aaatttatgc agaggaggaa gaactcaata tgaattctct
    12181 taatggtgcc ttgctcgcct tgatggcttt tgtcatgaca gtaccgcaaa tcatttttga
    12241 tggtggaatg atgaagactg tgacaagtct aaaagaaggt gcagtaattg cagatggatg
    12301 ggcaatggga aatggagtcg cacgttttgg tacaacagga atttttaccg caatcattat
    12361 ggcaattgtg actgttctta tttatcgtat gtgtgttaaa cataattggg ttattaaaat
    12421 gcctgaagct gttccagaag gagtttctcg tggatttacc gctttggttc cgggatttgt
    12481 tgttgcattt gttgttatct ttatcaacgg tcttcttgta gcaatgggaa cagatatttt
    12541 taaagtcatt gcaattccat ttggttttgt atccaatctg actaattcgt ggattggttt
    12601 aatgattatt tatctattga ctcaactact ttggattgta ggtatccacg gtgcgaacat
    12661 tgtttttgca tttgttagtc caattgctct tgctaacatg gctgaaaatg ctgctggcgg
    12721 gcacttcgct gttgcaggtg aattttctaa tatgtttgta attgcaggtg gttctggtgc
    12781 aactttagga ctatgtttat atattgcttt tgcctctaaa tctgaacagc ttaaagcaat
    12841 aggacgagca tctgtagttc cagccttatt taatattaat gaaccattaa tttttggatt
    12901 acctattatc tataatccag ccttggctat accatttatt ttagcaccaa tggttactgc
    12961 tactatttat tacgtagcga attctctaaa ctttattaag ccaattatcg cacaggttcc
    13021 atggccaact ccagtaggga ttggagcttt cttagggaca gcagatcttc gagctgtatt
    13081 agttgctcta gtatgtgcat ttgcagcatt cctagtctat cttccattca tccgtgtata
    13141 tgatcaaaaa ttggtgaaag aagagcaagg tatctaataa gtaaaaaagt atactcgatg
    13201 atagaatcca tatagtttaa ataatgagcg ttaacattag cagtgaagag gtctttttaa
    13261 gatattcttc tctgcttttt catatttttt cgctatgtag agtatgaagc aaaatagaaa
    13321 gtagttcgaa tagttgataa acagggaatt gctgttattt aaaaaaaaca ataaagtaaa
    13381 gtatgttatc gtagaatacg atacaatagt aaaaaagcaa aaagaatgcg ttttatattt
    13441 gggtttgagg cttataaaat aggataagcg tactgaaaac ttagatatga caaacaaaat
    13501 aaaattttta aaagctacag agaaacatgg tataatgtat ctaaaggagg tagcatatgt
    13561 ccttagatat agataaagaa aagatgacaa tcatgggaat agcttttgaa aaccgatccg
    13621 tttttaagag tgtttggtat gctttaagta caaatatgat tgaaggatgg cgacctacgg
    13681 ttagtgatgt tgaaaaatta cgagatgaag ctctttctct ggggatgact taatgaagcg
    13741 caactgttat gagtcttatg actatgttga tccggataat ttatataccg atccgggttc
    13801 ttcagttcta agaaataagc aggaaggaaa aaagcacgcg agcttgaata tcgtatgatt
    13861 gctagtaaga gtttgaaatt atttatcaat cctatcttag ttaatagttt aaaagtagtt
    13921 tgtttcacaa ctgcataagc ttagaaaagt tgaaaagtga taactcttta ataaactctt
    13981 ggtttgttaa aagatatttt gtccactaca tttttttcaa ttgactatta tttgtcatag
    14041 atataacaga tagaacattg cattcagaat aatgaaaaac gcatcaaaaa tggtgcgttc
    14101 tgtttttatg ctaccataaa aggaggaaaa caaattgaaa atttttaagg gagagtttta
    14161 tcgaatctct gtattaacag acaagctagt aaggttagaa tactctcgaa ctggaaattt
    14221 tgaggataga acgacacaac ttatctataa tagagatttt ggccaagttt cgttagatta
    14281 tatcgagaca tcaaatgtac tagatattat gacggactat tttcatctgc actttaataa
    14341 aggagaattt aacgccgaaa atttatttat agaattaaaa ggaaattttg ccgtatatgg
    14401 tagtcgctgg tattttggtg aatctattga aacgttaaaa ggaacagctc ggactctgga
    14461 taaggcagat ggagcaatct cgttagaaga tggaattatt agccgaaatg gtatagcctt
    14521 attggatgat tctcaaggat ttatttggga tgaacaatct ggttatattg agagagaaaa
    14581 tcaaattgac ctgtatttct ttgcctatgg gcatgattat agaggagcaa tcagagactt
    14641 ttaccatttg actggttcaa cacccttgtt gccaagatat gctttaggca attggtggag
    14701 tagatattgg ccttatacgt cggatgaata cttggattta atagacagat ttgaaacaga
    14761 gaaaattcca ttatctatcg gtgtgttaga tatggattgg catataactg acattccagc
    14821 tcgttttgga agtggctgga caggatatag ttggaataga aacttaatac caaatccaga
    14881 acagttattg caacaacttc atgatagaaa gttaaaactc tccttaaatg tccatcctgc
    14941 tgatgggata cgggcttatg aagaagctta tcctcaaatc gcaaaacggt tggggttaaa
    15001 tgtagaacta gaagaacctg ctatttttga tttttttaat ccctctttta gggaagccta
    15061 ctttaaagat gttcattatg agctagaaaa gcagggagta gatttttggt ggattgactg
    15121 gcaacaaggg acacaaggta tgctagatcc actttggctt ttaaaccatt atcactatca
    15181 ggatagttgt aaaaatgcag aaggtggttt gattttatca agatatgcag gtcctggtag
    15241 tcaccgctac cctgttggtt tttcagggga cactattatt agttggaatt ccttaagatt
    15301 tcaaccctat tttacagcga cagcatctaa tatcggttat agttggtgga gtcatgatat
    15361 cggtggacat atgttggggg attatgacga agagctacaa actagatggc tacagtttgg
    15421 tgtttttagt ccgattactc gattacatag ttctagaagt ccttttaata gtaaagaacc
    15481 ttggttcttt tcagaaacaa catctaagat tatgaagaaa taccttcgtt tgagacatca
    15541 gatgattccc tatctatata ccatgaatgt aaagacacat gaggaaggtg ccccattaat
    15601 cagtccaatc tattatttct atccagagaa tgatgagagc tataatgttc caaaccaata
    15661 cttttttgga acagaactga tggtggctcc cattgtagaa aagatggatt tgacattcca
    15721 atctgcaaaa gtagatgtat ggttccctga agatgaatgg tatgacttct tttcagagaa
    15781 aaaatacaca ggtggtgtga agttaagtgt ttatagggac atctcgacta cgcctgtgtt
    15841 tgcaaaaagt ggtgcaatca ttcccttggt tggttctgag ataggtatgg gtgttgattt
    15901 acctgaggtt gtagattggt atgtattccc agggaaacaa cattcttttg aaatgcttga
    15961 agatcaaaat ggtcaaagat ataaaacaag attatcaatc gattgggaaa tgggaatggt
    16021 agagttagca ttacaaggag attctagtat cgttccaagc aatagaaaac atagaattca
    16081 ttttaaagga acgaatgtgt ctataattga attgccaaat aagaatgata cagctaaatt
    16141 tgaatggaaa gataataaaa ggacatcttt aaatgacgaa gtttttagac tactaaagac
    16201 agcttctctt ccatatgaat taaaagatag attgttaaat caattcatca atgcgaaaaa
    16261 ctctcatgac ttaatgaata tcttgcatca tcaggataag gaattgagag ggcgtttgtt
    16321 ggaaatgata tttactagcc aaaactaatt gaaataatta gaaagtcatt tgtttagtgt
    16381 atatgaagat tctgtatggt attcgttatc actggtcaca ttgaaaatgt gaaataagca
    16441 cctacaacat agactttaca acacttcatg ttttatatca tggggtgttt ttctatttaa
    16501 gtcaaagccc atcaaaaaat ctcactattc atcaggttaa aggaaaccta atcatagtga
    16561 gattgtgaat tatagtagag tttgtatcgc ctcttcaatt tgttttgggt ttgtttttga
    16621 agaaaagcgt tcaaagactt gcccatcacg accaatgaga aacttagcga aattccattc
    16681 gattcgtttt cctagtgggc cagatttatg gtcttttaac cagacataga gagggtctgc
    16741 ttccttaccg ttgaccttaa tcttggcaaa acgtgggaag gtggtttgaa aatgtaggct
    16801 acagaaggcg ttgatttcct ctgcgctgcc gggtgcttgt cccataaact gattgcaagg
    16861 gaaatccaat atttcaaagc cctgatcttg atagcgttca tagagttctt gaagtccctg
    16921 gtactggggc gttaaaccac atccagtagc agtgttgaca atcaagagaa ctttaccacg
    16981 atagctatcc aagggagttg cttggttgtt ttggttcaaa acggaaaaat cataaagtga
    17041 agtcatgagg gcctcttttc tttttctaca ttgtaacagt ttttactttt ttcgtcgagt
    17101 cggatggaag atattgttga agaataagcc ttcaatatga tagaaaccgt ttacaaataa
    17161 ctttaaaaat ggtatgattt aattaaaccg gtttactttg ttgctataaa tctctaattt
    17221 gagatttagt tcctattgat tttacaatat gtttattgga gtgtatacat gcaaacaaaa
    17281 acaaagaagc tcattgtgag tttgtcttca cttgttttat caggattttt attaaaccat
    17341 tatatgacag ttggagcgga agaaacgact acgaatacca ttcagcaaag ccagaaggaa
    17401 gttcagtatc agcaaaggga tacaaaaaat ttagttgaaa atggtgattt tggtcagacg
    17461 gaggacggaa gcagtccgtg gacaggaagc aaagctcagg ggtggtcaac ttgggtagac
    17521 cagaagaata gttctgcaga tgcctcaact cgagtcattg aggctaagga tggggctatc
    17581 actatctcaa gccatgagaa attaagggca gcgcttcacc gtatggttcc tattgaagct
    17641 aagaaaaagt ataaactgcg tttcaagatt aaaacagatc ataaagtcgg gattgccaaa
    17701 gttcgtatca ttgaggaaag tggtaaggac aagcgattgt ggaattctgc aacgacgtca
    17761 ggaacaaagg actggcagac cattgaagca gactatagcc cgactttaga tgttgataaa
    17821 atcaagctgg agttattcta tgaaacagga actgggactg tttcctttaa ggatattgag
    17881 ctggtagagg tagcagacca gccttctgag gattctcaaa cagataaaca gcttgaggaa
    17941 aagattgatt taccaattgg aaaaaaacat gttttttctc ttgcggacta tacttataag
    18001 gtagaaaatc ctgacattgc ttcagtcaaa aatggaattt tagaacctct taaggaaggg
    18061 acaaccaatg tcattgtcag taaagatggc aaggaagtga aaaagattcc tttgaagatt
    18121 ctagcctctg ttaaggatgc atacacagac cgtttggatg actggaatgg catcatcgct
    18181 gggaatcaat actatgattc taaaaatgaa cagatggcca aattaaacca ggaattggaa
    18241 ggaaaggtag ctgatagcct atctagtatt tcaagtcagg cggaccgcac ctatttgtgg
    18301 gaaaaatttt caaattataa aatgtctgca aatctgactg ccacttatcg gaaattggag
    18361 gagatggcca agcaagtgac caatccttct tctcgttatt atcaagatga aactgtcgtt
    18421 cgaacagtca gggattccat ggaatggatg cataaacatg tctacaatag tgaaaagagc
    18481 attgttggga actggtggga ttatgaaatc ggtacacctc gtgccatcaa caataccttg
    18541 tctctgatga aagaatactt ctctgatgag gaaattaaaa aatatacaga tgtgattgaa
    18601 aaatttgtac cagatcccga acatttccga aagacgactg ataacccatt caaggctcta
    18661 ggtggaaact tagttgatat gggaagggta aaagtaatag ctggtttact gcgtaaggat
    18721 gatcaagaaa tttcttctac cattcgctcg attgagcaag tgttcaagtt ggtagaccaa
    18781 ggtgaaggtt tttatcaaga tggatcctat atcgaccaca ccaatgttgc ctatacgggt
    18841 gcttatggga atgttttgat tgatggcctg tctcaactgt tgccagtcat tcaaaagacc
    18901 aagaatccaa tcgataaaga taaaatgcaa accatgtacc actggattga taaatcgttt
    18961 gctcctttgc tggtgaatgg agagctgatg gatatgagtc gtggacgctc gatcagtcgt
    19021 gcaaatagcg aggggcacgt ggccgcagta gaagtactaa gagggattca ccgaatagcg
    19081 gatatgtctg aaggagaaac caaacaacgt ttgcagagtc ttgtgaagac cattgttcaa
    19141 tcggatagtt attatgatgt ctttaagaat ttgaagactt ataaggatat cagtttgatg
    19201 caatccttgt taagtgatgc aggagtcgca agtgttccaa gaacaagtta cctatctgcc
    19261 tttaacaaga tggataaaac agccatgtac aatgcagaga aagggtttgg atttggcttg
    19321 tcactctttt ccagccgtac cttgaattac gaacacatga acaaggaaaa taaaagtggt
    19381 tggtatacga gtgatgggat gttctatctt tacaatggcg atttgagtca ctatagcgat
    19441 ggctactggc caacagttaa tccatataag atgcctggta caacagagac ggatgctaag
    19501 agagcggata gcgatacagg taaagttcta ccgtctgctt tc
//
LOCUS       DAVBCW010000029        19422 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVBCW010000029 DAVBCW010000000
VERSION     DAVBCW010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19422
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.029"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            <1..661
                     /locus_tag="VQ514_000887"
     CDS             <1..661
                     /locus_tag="VQ514_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2499665.1"
                     /translation="FYLNKGKVYGFVGPNGAGKTTIIKMILGILKPDSGKITIFNQTV
                     EQNSKNILSRIGLVLGPSFYGHLDAYKNLKLIANMKGLSLDTERLNEYLSMVGLKDVK
                     KKKVKNFSMGMKQRLSIAASLLGSPEILIWDEPINGLDPQGVIEIRSLIRFLQEKKGI
                     TFLISSHILSELDKVISDIIIINYGKVEFFGSCHYLLQKYNCRNLEEAYLACLAGGEY
                     D"
     gene            654..1391
                     /locus_tag="VQ514_000888"
     CDS             654..1391
                     /locus_tag="VQ514_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2499666.1"
                     /translation="MIKLEFLKQKKSILWFVLIFPIILNALLYIDLTFRYRGYLLVHQ
                     NELALSNWQLIFKEQTIFYFSELFYLVLSLIIYEVFAVEFKNDAWLTVISLPFRNKYT
                     INSKLLITVVYTFTFWLSDYISLYVIGKAIDNSLEIGLIFFLKTFTIQLISSLMIMLL
                     YFLTLVLIRKISGIIPIGIIMMILTISIYYNDYNFKIYLPFTYLSHAFRVTESQFYMI
                     LLSNIIIIVLFYILIRKLNERSFEMKL"
     gene            1404..2129
                     /locus_tag="VQ514_000889"
     CDS             1404..2129
                     /locus_tag="VQ514_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499667.1"
                     /translation="MKLLKNELIKSKIFLFIIVDICIQILVILAIKTYILDISAPYSE
                     LDYNKYWYVLHALIYMPMIFPIQILYQNLREALIEDNNNGWNIMVINTNNLVKIIYIK
                     VTINIVRCFICYFVYTIFSLIQLGGMGTDMLLTNIIFPNIIFPNIMSFLLFLPIAIFM
                     QICCIRFDSILAKALPNILLILIVLITFQSDWNIFIPATYYYTEIQSTTNLGIKLLVC
                     IWIMGFEFSLLPKLIKLKEQNLV"
     gene            2672..2893
                     /locus_tag="VQ514_000890"
     CDS             2672..2893
                     /locus_tag="VQ514_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2499668.1"
                     /translation="MYRRLRDLREDHDLTQKQIAKILSFTNSAYAKIERGEHTLTADV
                     LVTLSNFYDVSTDYLLELTDFPDKIRFRK"
     gene            3410..3649
                     /locus_tag="VQ514_000891"
                     /pseudo
     CDS             3410..3649
                     /locus_tag="VQ514_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            3793..5022
                     /locus_tag="VQ514_000892"
     CDS             3793..5022
                     /locus_tag="VQ514_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET2499669.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            5042..7738
                     /gene="ppc"
                     /locus_tag="VQ514_000893"
     CDS             5042..7738
                     /gene="ppc"
                     /locus_tag="VQ514_000893"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HET2499670.1"
                     /translation="MSLQKLENSSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATNLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYEIILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    7821..8038
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8151..9155
                     /locus_tag="VQ514_000894"
     CDS             8151..9155
                     /locus_tag="VQ514_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET2499671.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNVTGVSDHNPAQQQ
                     IELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTIKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            9224..10090
                     /locus_tag="VQ514_000895"
     CDS             9224..10090
                     /locus_tag="VQ514_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2499672.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            10087..10845
                     /locus_tag="VQ514_000896"
     CDS             10087..10845
                     /locus_tag="VQ514_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2499673.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEDLTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            10935..12695
                     /gene="dnaG"
                     /locus_tag="VQ514_000897"
     CDS             10935..12695
                     /gene="dnaG"
                     /locus_tag="VQ514_000897"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HET2499674.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVTFMEAVQILGQRVGIEVEKPLYSEQ
                     KPASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKTSEIYLMEGFMDVIAAYRAGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAAILKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFIEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMERISRPTPITMPVT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            12698..13807
                     /gene="rpoD"
                     /locus_tag="VQ514_000898"
     CDS             12698..13807
                     /gene="rpoD"
                     /locus_tag="VQ514_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HET2499675.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            13821..14150
                     /locus_tag="VQ514_000899"
     CDS             13821..14150
                     /locus_tag="VQ514_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HET2499676.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            14265..15308
                     /gene="cpoA"
                     /locus_tag="VQ514_000900"
     CDS             14265..15308
                     /gene="cpoA"
                     /locus_tag="VQ514_000900"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HET2499677.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAVLKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            15324..16649
                     /locus_tag="VQ514_000901"
     CDS             15324..16649
                     /locus_tag="VQ514_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET2499678.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGILIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGALYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFRTVLYLPQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            16680..16856
                     /locus_tag="VQ514_000902"
     CDS             16680..16856
                     /locus_tag="VQ514_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET2499679.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            16915..17049
                     /locus_tag="VQ514_000903"
     CDS             16915..17049
                     /locus_tag="VQ514_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HET2499680.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            17113..18423
                     /gene="obgE"
                     /locus_tag="VQ514_000904"
     CDS             17113..18423
                     /gene="obgE"
                     /locus_tag="VQ514_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HET2499681.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLSENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            18433..18591
                     /locus_tag="VQ514_000905"
     CDS             18433..18591
                     /locus_tag="VQ514_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499682.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
BASE COUNT         6077 a         3248 c         4141 g         5956 t
ORIGIN      
        1 attttattta aataagggaa aagtatatgg ttttgttgga ccaaatgggg ctggaaaaac
       61 tacaattatc aaaatgattc tagggatttt aaaaccagat tctgggaaaa taactatttt
      121 taatcaaacc gtagaacaga attccaaaaa tatattatct aggataggat tagttttggg
      181 accttcattt tatggtcatc ttgatgctta taaaaatttg aagttaatag caaatatgaa
      241 agggcttagt ttagatactg aaagacttaa tgaatatcta tcaatggtag gattaaaaga
      301 tgttaaaaag aaaaaagtga aaaatttttc aatgggcatg aaacaacgtc taagtatagc
      361 tgcatcattg ttaggtagtc cagaaatatt aatttgggat gaaccaatca atggacttga
      421 tccacagggt gtaattgaaa ttagaagttt aatccgtttt ctacaggaga aaaaaggtat
      481 tacattttta atatcaagtc atattcttag tgaactggat aaagtgatat ctgatattat
      541 aattataaat tatgggaaag ttgaattttt tggttcttgc cactaccttt tacaaaagta
      601 taattgtcga aatctggaag aagcctactt agcttgttta gctggaggtg aatatgatta
      661 aattagagtt tctaaagcag aaaaaaagca tcttatggtt tgttcttatt tttccaataa
      721 ttctgaatgc tttgttatat atagacttaa cttttaggta tcgaggttat ttattggtgc
      781 atcaaaatga acttgcttta tctaattggc agttaatttt taaagaacaa acaatatttt
      841 atttttctga actattttat ttagttctat ctttaataat ttacgaagtg tttgcagtag
      901 aatttaaaaa tgatgcatgg ttaaccgtaa tttcattgcc atttagaaac aagtacacaa
      961 taaattctaa attactcatt acagtggttt acacatttac tttttggttg tctgattata
     1021 ttagcttata cgttattggg aaggctattg ataacagttt agagattggt ttgattttct
     1081 ttttaaaaac atttaccatt caattaattt cctcgttgat gataatgtta ttatattttc
     1141 taactttagt acttataagg aaaatttctg gaataatacc aattggtatt ataatgatga
     1201 ttttaacaat tagcatatac tacaacgatt ataattttaa gatttactta ccatttactt
     1261 atctttctca tgcttttaga gtgactgaaa gtcaatttta tatgatttta ctatctaata
     1321 taataatcat cgtacttttt tatatattaa ttcgtaaatt gaacgaaaga agttttgaga
     1381 tgaaacttta ataggtaata aaaatgaagt tacttaaaaa tgaattgatt aagagtaaaa
     1441 tatttttatt tattattgtt gacatctgta ttcagattct agtcattcta gctataaaaa
     1501 catatatttt agatatttcg gcaccatact cagagcttga ttataataag tattggtatg
     1561 ttttacacgc tttaatatat atgccaatga tttttcctat acaaattttg tatcaaaatc
     1621 taagagaggc attgattgaa gataataata atggttggaa tatcatggtg attaacacta
     1681 ataatctcgt gaaaatcatc tatataaaag taacgataaa tatcgtgaga tgttttattt
     1741 gttattttgt ttatacaatt ttttctttaa ttcaattagg tggtatggga actgatatgt
     1801 tattaaccaa tataatattt cccaatataa tatttcccaa tataatgtca tttctgttat
     1861 ttttacctat agctatcttt atgcaaattt gctgtataag atttgattcc attttagcaa
     1921 aggccttacc taatattttg ctgatactta ttgttttaat cacgtttcag agtgattgga
     1981 atatatttat acctgcaact tattattata cagaaattca gtcaactaca aacttaggta
     2041 ttaaattatt agtttgtatc tggattatgg gatttgaatt ttcccttctt ccaaaactaa
     2101 taaaattgaa agaacaaaat ctagtttaaa tgttgctcga aaattaaata attgaatacg
     2161 attaacaatg aaacaataca acgtgagtca tgacttcaaa gtaattgatt ttatgattct
     2221 ggtttcttac gcttaattaa gcaagttgta ttcactttat caaattgtta ttactctaaa
     2281 aagcgtctaa cagctataga gtgtctgttt gcttctcttt acttttattt taaataattc
     2341 attaaccttc cgtaagtgcc caataataag gtgtgttgaa gaagtctgag ataaaatagt
     2401 tctcagtcac aaaaaaagct atagattccc attggtgaac agctccctgt caagtagaca
     2461 gtaaaataat ataaaatctt aagcgacctg attcctgaat tccagtggag tcaggttgtt
     2521 ttttaaccgt ctttcttaat attgtctcac ctataataga tatgcttatg agatatgttt
     2581 aataaattaa ttttagttca aaatgtccag ttaggacact ttgaatgatt tcttagtttt
     2641 atatattata cttttctcag gagaaagcta gatgtacaga cgtttgagag atttgaggga
     2701 agatcacgat ttgacccaaa aacaaatagc taaaatactt tcgtttacaa actcagctta
     2761 tgctaaaatt gaacggggtg agcatacgtt aacagcagat gtattggtaa ctctctcaaa
     2821 cttttacgat gtcagtacag actacctatt ggaattgaca gattttcctg ataaaattcg
     2881 ctttagaaaa taatctcctc aatttcatag agtgtgaaaa tgaggagatt ttttatttgt
     2941 tctttgaaaa ttaaatagtc caaaatggta ctttcctcat ttgtggagca aatttgaatg
     3001 gctcgccatg ataagagcga ttttaaagcc atcaataaaa tagagcgata ctttatatgc
     3061 catgatacaa atgatataca atgatacttc tgaccgttca ggctgccatc gtaaaagagc
     3121 agcaagtgaa attcttatga tgacttcatc agtcatgcca cgttgaatgt gtgagtttgt
     3181 tagagaaacg caattaatcc tcaaaaggtt ccccgaacct tttgagttcc acagacgcat
     3241 cacgtggagt gtgtaagctt gttgctaaaa gcgtaaaaac cttggaacga aaggaataat
     3301 agacttcctg cgaaacaaaa atacaataca ataaaactat ggatgatgaa gcaagtaaac
     3361 aattaagcga tagccgtttc aagagccttg taggtgttca gcgcacgaga tgttagctgt
     3421 gttaaaaaca gcttatcaac gcaacgtaaa cgcgcaaaag gtggacgaaa aaccaaatta
     3481 agcctagacg atctcctcat ggtaactatt caatacatgc gagaatagcg cacttatgaa
     3541 caaattgcgg ctgattttgg cattcacgaa agcaacataa ttagtcggag gtcaatggat
     3601 tgaagcaact cttattcaaa atggttttac gatttcaaat tctgccttaa ttctgtaaaa
     3661 acagtaaaat tcgaaggatt gtaaggtaag agtttttttc tttctgaaaa aatggtataa
     3721 tagcaatcaa aactagaaaa taaaacggaa tttggaacag atttgtctgt atcctagtag
     3781 agtggtgata ctatgaagat tagtaagagg cacttattaa attattccat cttgattccc
     3841 tacttgcttt tatctatttt gggcttgatt gtggtctatt cgaccaccag tgctatttta
     3901 attgaagaag gcaagagcgc cttgcagttg gttcgaaacc aaggaatctt ttggattgtt
     3961 agtttgatac tgattgcctt aatttataaa ttgagactag attttttgag aaatgagcga
     4021 ctaatcattt tagttatatt aatagaaatg cttttattgt tcttggctcg ttttattggt
     4081 atttcagtaa acggggcata cggttggatt tcggttgcag gagtaactat tcagccagct
     4141 gagtacttaa aaatcattat tatttggtat ttagctcacc gattctccaa acagcaagaa
     4201 gaaatagcta cttatgattt tcaagttttg actcaaaatc aatggcttcc ccgtgctttt
     4261 aatgattggc gattcgttct cctagttctg attggaagtt tgggaatttt ccctgattta
     4321 ggaaatgcga ctattttagt cttggtttcc ttgattatgt atacagttag tggaatcgct
     4381 tatcgctggt tttcaaccat tctggcgctc gtatctgccg cttctgtctt tgtcttgacc
     4441 actatcagcc taatcggtgt tgagaccttt tcaaaaattc cagtatttgg ctatgtagcc
     4501 aaacgcttta gtgccttttt taatcctttt gccgatcgtg ctgatgcagg tcaccagtta
     4561 gctaattctt attttgccat ggtcaatggt ggttggtttg gtctaggtct tggaaactcg
     4621 attgaaaaac gaggttattt gccagaagct catacagact ttgtcttttc tatcgtgatt
     4681 gaagaatttg gctttgttgg tgccagtctt attttagctc tcttgttttt catgattttg
     4741 cggattatct tggtcggtat tcgagcggag aatcctttca atgccatggt tgcactcggt
     4801 gtcggaggga tgatgttggt tcaggtattt gtcaatatcg gagggatttc gggcttgatt
     4861 ccatctacag gagtaacctt ccccttctta tcccagggtg gaaatagtct tctagtctta
     4921 tcagtggcag tagcctttgt cttaaatatt gatgccagtg aaaaacgcgc taagttgtac
     4981 cgagaattgg aaaatcaacc aatgaacctt ctgttgaagt aggataaaga aaggatagtt
     5041 tatgtctctt caaaaattag aaaattctag taataaaagt gttgtgcaag aagaagtctt
     5101 gattctaaca gaattactgg aagatattac taaaaatatg cttgccccag agacctttga
     5161 aaaaataata cagttgaaag aattatcaac gcaggaagat tatcaaggtc taaaccgtct
     5221 agtgactagc ttatcaaatg atgaaatggt ctatatttca cgctatttct ctatcttgcc
     5281 tcttttgatt aatatttcag aggatgtgga tttagcttat gaaatcaatc atcaaaataa
     5341 tattgatcag gactatttag gtaaattatc tacaacgatt aaattggtag cagaaaagga
     5401 aaatgccgtt gagatcctag aacacttgaa tgttgttccg gttttgacag cccatccaac
     5461 acaagtgcaa cgcaaaagta tgttggattt aacaaatcat attcatagtc ttttgcgtaa
     5521 ataccgtgat gttaagttgg ggttgatcaa taaagataaa tggtacaatg atttgcgtcg
     5581 ttacatcgaa attatcatgc agacagacat gattcgtgag aaaaaattaa aagtgactaa
     5641 cgaaatcacg aatgctatgg aatattataa cagctccttt ttgaaagctg tacctcattt
     5701 gacgacggag tataagcgct tagcgcaagc gcatggtctg aatttaaaac aggctaaacc
     5761 aatcaccatg ggtatgtgga taggtggtga ccgtgatgga aatccatttg ttacagcaaa
     5821 gaccttgaag cagtctgcac tcactcaatg tgaagtcatc atgaactact atgataaaaa
     5881 gatttaccaa ctttatcgtg aattttctct ttcaactagc attgtcaacg tcagcaagca
     5941 agtcagagaa atggctcgtc aatccaagga taactcgatt taccgcgaaa aagagcttta
     6001 ccgtcgtgcc ttgtttgata ttcaatcaaa aattcaggca actaaaacct atctgattga
     6061 ggatgaagaa gttgggactc gttatgaaac cgccaatgat ttctacaagg atttgattgc
     6121 cattcgagat tctctactag agaataaggg cgagtccttg atttcaggtg attttgtgga
     6181 attattgcag gcagtagaga tatttggttt ttacctagca tcaattgata tgcgacaaga
     6241 ctctagcgtc tatgaagcct gtgtggcaga actcttgaaa tcagcaggaa ttcattctcg
     6301 ttatagcgag ttgagcgaag aagaaaagtg tgaccttctt ttgaaagaat tagaagaaga
     6361 tccccgaatt ctttctgcga ctcacgcaga aaaatcagaa ttattagcaa aagaactagc
     6421 tatttttaag acggctcgtg ttttgaaaga taagttggga gatgatgtca tccgtcagac
     6481 catcatttca catgcaacca acctttctga tatgctagaa ttagctattc tgttaaaaga
     6541 agtaggactg gtggatacgg aaagggcgcg tgttcagatt gttccccttt ttgaaacaat
     6601 tgaagacttg gatcattcag aggaaacaat gagaaaatat ctttctctta gccttgccaa
     6661 aaaatggatt gactcacgaa ataactacca agaaatcatg cttggctact ctgacagtaa
     6721 taaagatggc ggttacttgt catcatgttg gaccctctac aaggctcaac aacaattgac
     6781 tgctattgga gatgaatttg gcgttaaggt taccttcttc catggtcgtg gtggtactgt
     6841 cggtcgtggt ggtgggccaa cctatgaagc cattacatct caaccgctca agtctatcaa
     6901 ggatcgtatc cgtttgacgg agcagggtga agtaattggg aataaatacg gtaacaaaga
     6961 cgccgcttac tataaccttg aaatgctagt atcggcagct attaaccgta tgattactca
     7021 gaagaagagc gataccaata ccccaaatcg ttatgaagcc attatggatc aagtagtgga
     7081 ccgtagttac gatatctacc gtgatttggt ctttggtaat gagcatttct atgattattt
     7141 cttcgagtca agtccaatca aggctatttc aagttttaat attggttctc gtccagccgc
     7201 tcgtaagact attactgaaa tcggtggttt gcgtgccatc ccttgggtat tctcatggtc
     7261 acagagtcgt gttatgttcc ctggatggta cggggttggt tcaagcttca aggaatttat
     7321 caataaaaat ccagagaata ttgctatctt acgagatatg taccaaaatt ggcctttctt
     7381 ccaatcgctt ctttcaaatg ttgatatggt tttgtcaaaa tcaaatatga atattgcttt
     7441 tgaatatgct aaactttgtg aagacgagca agttaaggcc atctatgaga ttattttaaa
     7501 tgaatggcaa gttactaaga acgttatctt ggctattgaa ggacatgacg aactcttagc
     7561 tgacaatcca tatctaaaag ctagtctgga ttaccgtatg ccttacttta atattctcaa
     7621 ctatattcag ttagagttga ttaaacgcca acgtcgtgga gaattgtcca gtgatcaaga
     7681 acgattgatt catatcacca tcaacggaat tgcgacagga ttgcgtaatt caggttgata
     7741 attttcaaga gtgaatgcta aaagtgaata tcaaaaaaat tctaatagac tattgacaag
     7801 tagtttaaaa atgatataat ttaaccattc agaaaagtaa tcatacaaac tttttagaga
     7861 gtctgtggta gctgaaaaca gataagtggc gatgatgaaa attgggctga atgctattta
     7921 gaatttgaaa ttataaaaat tcggtaagca caccttacag tgcatctcgt tattgcgaga
     7981 ctgagcgata gggaaattcc ctataattga ggtggtaccg cgcatcgacg tcctcacaca
     8041 agttttttgt gtgaggattt ttttgatgga ggttagtatg gaaagaaaac gatggcgtcg
     8101 cttgtttaga taagtgaaat atgttaaagg aaataaaaag gagaaacaga atgaaaaata
     8161 aacgtttaat tggaattatt gctgcattag cagtcttagt agcaggaagc ttgatttatt
     8221 cttcaatgaa taaatcagaa gctcagaata ataaggatga gaagaaaata accaagattg
     8281 gtgtgcttca atttgtgagc catccatccc ttgatttgat ttataaaggg atccaagatg
     8341 gacttgcaga agaaggatat aaagatgatc aagttaaaat tgattttatg aactcagaag
     8401 gtgatcaaag taaggttgcg acaatgagta aacaattggt tgcaaatggg aatgaccttg
     8461 tggttggtat tgcaacacca gcagcccaag ggttggctag tgcaacaaaa gacctaccag
     8521 ttatcatggc cgctattaca gacccaattg gtgctaactt ggttaaagat ttgaaaaaac
     8581 caggtggtaa cgttacaggg gtatctgacc acaatccagc tcaacaacaa attgaactca
     8641 tcaaggctct gacaccgaat gtgaaaacaa tcggagctct ttactcaagt agcgaagaca
     8701 attcaaaaac acaggttgaa gaatttaagg cttatgctga aaaagcaggt ctgacagtgg
     8761 aaacatttgc agttccttca acaaatgaaa ttgcctcaac tgtcactgtt atgactatca
     8821 aggtagatgc tatttgggtt ccaattgata acaccattgc atcaggattt ccaacggttg
     8881 tctctagcaa tcaaagttct aagaaaccaa tttatcccag tgcgacagct atggtagaag
     8941 taggtggttt ggcatcagtt gtaattgacc aacatgacct tggtgtggca acaggtaaaa
     9001 tgattgtgca agtcttgaaa ggtgcaaaac cagccgatac cccagtcaat gtcttttcaa
     9061 ctggtaagtc agtcatcaat aaaaaaatag cacaagaact aggtattact attcctgagt
     9121 ctgttctcaa agaagcagga caagtcatcg aataaataag gtagggagga ggagactctt
     9181 cccatttttt aaaaagatga atatagaaag gattaaataa aatatgattg tttccattat
     9241 ttctcaagga tttgtctggg ctattctagg tctgggaatc tttatgacat ttaggatttt
     9301 aaactttcca gatatgacga cagaaggttc cttccctctt gggggagctg ttgctgtcac
     9361 tttgataacc aaaggcgtga acccattttt agcgacactt gttgctgtag gagcaggttg
     9421 tttggctgga atggcagcag gccttcttta tacaaaaggg aagatcccaa ccttgctctc
     9481 agggattttg gtgatgactt cttgtcactc aatcatgctc ttgattatgg gacgtgcgaa
     9541 tttaggcctg cttggaacca agcaaattca ggatgttttg ccttttgatt cggatttgaa
     9601 tcaactcttg acaggtctca tctttgtgag cattgttatt gctctcatgc tctttttctt
     9661 ggacactaaa ctcggacaag cctatattgc tacaggggat aatcctgata tggctagaag
     9721 tttcgggatt catactggac gcatggagct catgggcttg gtcttatcaa atggtgtgat
     9781 tgcccttgca ggtgccctca ttgctcagca agaaggctat gccgatgtgt ctcgagggat
     9841 cggggttatc gttgtggggc ttgcaagttt gattattgga gaagttattt tcaagagttt
     9901 gagcttggca gagcgtttgg ttactatcgt tgtaggttct atcgcttatc aatttttagt
     9961 gtgggcagtt atcgcacttg gctttaatac aagttacctt cgtttataca gtgccttgat
    10021 tttagcagtc tgcctcatga ttccaacatt taagcaaaca atcttgaaag gagccaagtt
    10081 aagcaaatga cagcaattgt agaattaaaa aatgcaacca aaattgttaa aaatggcttt
    10141 gatgaagaaa agattatttt aaatgatgtt tccttagaaa tttttgaacg ggactttatc
    10201 acgattttgg gcggaaatgg tgctggaaaa tcaaccctct ttaacactat agcagggacc
    10261 ttatcactaa ctagtggaac tatccgtatt ttaggtgaag atctcactaa gttttcaccc
    10321 gagaagcgtg ccaagtacct gtctcgtgtc ttccaagatc caaagatggg gacagctccc
    10381 cgtatgacgg tcgctgaaaa tcttttaatc gccaagtttc gtggtgagaa gcgtggattg
    10441 ttaccacgac gcttgactag ctataaggat gaatttcagg caaccattga aaaagtagga
    10501 aatggtcttg agaaacactt gaatacaccg attgagttct tatcaggtgg acaaagacag
    10561 gctttgagtc tcttgatggc aaccttgaag cgacctgaat tactcctgtt agatgagcat
    10621 actgctgccc tggatccaaa gactagtgtt gctttgatgg aattgacaga tgaatttgtt
    10681 aagaaagatc agctaacagc ccttatgatt actcatcata tggaagatgc tctcaaatac
    10741 ggcaatcgct tgattgtcat gaaagaagga cggattatcc aagatttaaa ccaagaagaa
    10801 aaagcaaaaa tgaaaatctc tgattattat caactctttg aataaagtag ggaaaatgag
    10861 tgaaatggtt tacttttttt ctgaaataaa gtatactata taaagtaaac tatgataaca
    10921 tggaggtatt gtgtatggtt gacaaacaag tcattgaaga aatcaaaaac aatgccaaca
    10981 ttgtggaagt cataggagat gtgatttctt tacaaaaggc aggacggaac tatctagggc
    11041 tctgtccttt tcatggtgaa aaaacacctt ctttcaacgt tgtagaggac aagcagtttt
    11101 accactgttt tggttgtggt cgctcaggtg atgtctttaa attcatcgag gagtaccaag
    11161 gggttacctt tatggaggct gtccaaatct taggtcagcg tgtcgggatt gaggttgaaa
    11221 aaccgcttta tagtgaacag aagccagcct cgcctcacca agctctttat gatatgcacg
    11281 aagatgcggc taaattttac catgctattc tcatgacaac gactatgggc gaagaggcca
    11341 gaaattacct ttatcagcgg ggtttgacag atgaagtgct taaacatttt tggattggtt
    11401 tagcacctcc agaacgaaac tatctctatc aacgtttgtc tgatcagtat cgtgaagagg
    11461 atttactgga ttcaggcctg ttttatctct cggatgccaa tcaatttgta gacacctttc
    11521 acaatcgcat tatgtttccc ctgacaaatg accaaggaaa ggtcattgcc ttctcaggtc
    11581 gtatctggca aaaaacggat tcacaaactt ctaagtataa aaacagccga tcgactgcaa
    11641 tttttaacaa aagttacgaa ttatatcata tggatagggc aaaaaaatct tctggaaaaa
    11701 ctagtgagat ttacctgatg gaaggattca tggatgttat tgcagcctat cgggctggaa
    11761 tcgaaaatgc tgtggcgtcg atgggaacgg ccttgagtcg agagcatgtt gagcatctga
    11821 aaaggttaac caagaaattg gttcttgttt acgatggaga taaggctggg caagccgcga
    11881 tattgaaagc attggatgaa attggtgata tgcctgtgca aatcgttagc atgcctgata
    11941 acttggatcc tgatgagtat ctacaaaaaa atggtccaga agacttggcc tatctattaa
    12001 caaaaactcg tattagtccg attgagttct acattcatca gtacaaacct gaaaacagtg
    12061 aaaatctgca ggctcagatt gagtttattg aaaaaatagc tcccttgatt gttcaagaaa
    12121 agtccatcgc tgctcaaaac agctatattc atattttagc tgacagtctg gcgtcctttg
    12181 attataccca gattgagcag attgttaatg agagtcgtca ggtgcaaagg cagaatcgca
    12241 tggaaagaat ttccagaccg acgccaatca ccatgcctgt caccaagcag ttatcggcta
    12301 ttatgagggc agaagcccat ctactctatc ggatgatgga atcccctctt gttttgaacg
    12361 attaccgttt gcgagaagac tttgcatttg ctacacctga atttcaggtc ttatatgact
    12421 tgcttggcca gtatggaaat cttcctccag aagttttagc agagcagaca gaggaagttg
    12481 aaagagcttg gtaccaagtt ttagctcagg atttgcctgc tgagatatcg ccgcaggaac
    12541 ttagtgaagt agagatgact cgaaacaagg ctctcttgaa tcaggacaat atgagaatca
    12601 aaaagaaggt gcaggaagct agccatgtag gagatacaga tacagcccta gaagaattgg
    12661 aacgtttaat ttcccaaaag agaagaatgg agtaataatg gcaacaaaac aaaaagaagt
    12721 aacaacattt gacgtacagg tagcagaatt tatccgtaat cataagcaaa aagggacagc
    12781 aacagatgat gaaatcaatg caagtctggt tattcctttt accttggacg ctgatgggat
    12841 tgaagatctc ttgcaacgga ttcaggatgc agggatttct atcacagata acgaaggaaa
    12901 tccaagtgcg cgtgttctta gcaatgaaga agaaccagaa ctcagcgatg aggacttgat
    12961 tggatcaact tctgctaagg tcaatgaccc tgtccgtatg tacttgaaag aaataggggt
    13021 cgttcctctc ttgaccaatg aagaggagaa agagttggca ctggctgttg aagctggcga
    13081 tatcgaagcc aaacaacgtc ttgcggaagc caatcttcgt ttggttgttt ccattgccaa
    13141 acgctatgtc ggtcgtggca tgcagttcct tgacttgatt caagaaggaa atatgggctt
    13201 gatgaaggcg gttgacaagt ttgactattc taaagggttc aagttttcaa cttatgcaac
    13261 ttggtggatt cgtcaggcta tcactcgtgc tattgcggac caagctcgta ccatccgtat
    13321 cccagttcac atggttgaaa ctatcaataa attggttcgt gaacagcgga atctccttca
    13381 agaattgggg caagatccga caccagaaca gattgctgaa cgaatggata tgacacctga
    13441 taaggttcgt gaaatcttga agattgccca agaaccagta tctcttgaaa ctcctatcgg
    13501 tgaagaggac gatagccacc ttggagactt tatcgaagat gaagtgattg aaaatccagt
    13561 ggattatacg actcgtatcg tcttgcgtga gcaattggat gaaatcttag atactcttac
    13621 agaccgtgaa gaaaatgttc tgcgtctacg ttttggacta gatgatggaa aaatgcgcac
    13681 acttgaagat gtggggaaag tctttaacgt aactcgtgag cgtatccgtc agattgaagc
    13741 aaaggctttg agaaaactac gccaaccaag tcgcagcaaa ccgcttcgtg attttattga
    13801 agactaagag tgaggaaaat atggcttata cagaagagca aattgaaaac atcaaaacac
    13861 ggattttaac agccttggaa gaagtcatcg accctgagtt gggaatcgat attgtcaatc
    13921 ttggtttgat ctatgagatt cgttttgacg gtgacacagg gcaaacagag attgacatga
    13981 ctttgacaac tatgggttgt cccttagcag accttttgac agaccagatt tatgatgcta
    14041 tgatagaggt tccagaagta acggatactg aggttaaatt agtctggtat ccagcctgga
    14101 cagttgaaaa aatgagtcgc tatgcccgca ttgccctagg cattaagtaa acatataaaa
    14161 gcatgtgaga gactagagga aaagcgagga aatttcccct cttttcctct agtctctcct
    14221 ttcttttgct gattttattc aaagaaaatg atataatagt agttatggag aaaaagaaat
    14281 tacgcatcaa tatgttgagt tcaagtgaga aagtagcagg acagggagtt tcaggtgctt
    14341 accgtgaatt agttcgtctt cttcaccgtg ctgccaagga ccaattgatt gttacagaaa
    14401 atcttccaat cgaggcagat gtgactcact ttcatacgat tgattttccc tattatttat
    14461 caaccttcca aaagaaacgc tcagggagaa agattggcta tgtgcatttc ttgccagcta
    14521 cacttgaagg aagtttgaaa attccatttt tcttaaaggg aattgtgaaa cgctatgtat
    14581 tttcttttta caaccggatg gagcacttgg ttgtggtcaa tcctatgttt attgaggatt
    14641 tggtagcagc tggtattcca cgtgaaaaag tgacctatat tcctaacttt gtcaacaagg
    14701 aaaaatggca tcctctacca caagaagagg tagtcagact gcgcacagat cttggtctta
    14761 gtgacaatca gtttatcgta gtaggtgctg ggcaagttca gaaacgtaaa gggattgatg
    14821 actttatccg tctggctgag gaattgcctc agattacctt tatctgggct ggtggcttct
    14881 cttttggtgg tatgacagat ggttatgaac actataagaa aattatggaa aatcccccta
    14941 aaaatttgat ttttccaggc attgtatcgc cagagcggat gcgcgaattg tatgctctag
    15001 cggatctttt cttgttgcct agttacaatg agctctttcc tatgactatt ttagaagctg
    15061 cgagttgtga ggctcctatt atgttgcgtg atttagatct ctataaggtg attttggagg
    15121 gaaattatcg ggcgacagcg ggtagagaag agatgaaaga ggctattttg gaatatcaag
    15181 caaatcctgc tgtcttaaaa gatctcaaag aaaaggctaa gaatatttcc agagagtatt
    15241 ctgaagagca tctgttacaa atctggttgg acttttatga gaaacaagcc gctttaggga
    15301 gaaagtaaaa agtgaggtaa tctatgcgaa ttggtttatt tacagatacc tattttcctc
    15361 aggtttctgg tgttgcgacc agtattcgaa ccttgaaaac agaacttgaa aagcagggac
    15421 atgctgtttt tatctttacg acgacagata aggatgtcaa tcgctacgaa gattggcaaa
    15481 ttatccgcat tccaagtgtt cctttctttg cttttaagga tcgtcgcttt gcctaccgag
    15541 gttttagcaa ggcacttgaa attgctaaac agtatcagct agatattatc catactcaga
    15601 cagaattttc tcttggcctg ttggggattt ggattgcgcg tgaattgaaa attccagtca
    15661 tccataccta tcacacccag tatgaagact atgtccatta tattgctaag gggatattga
    15721 tccggccgag tatggtcaag tatctggtta gaggtttcct gcatgatgtg gatggggtta
    15781 tttgccctag tgagattgtc cgtgacttgc tatctgatta taaggtcaag gttgaaaaac
    15841 gggtcattcc tactgggatt gaattagcca agtttgagcg tccggaaatc aagcaggaaa
    15901 atttgaaaga actgcgtagt aaactaggga ttcaagatgg tgaaaagacg ttgcttagtc
    15961 tttcgagaat ctcctatgaa aaaaatattc aagcagtttt agcagccttt gctgatgttc
    16021 tgaaagagga agacaaggtt aaactggtag tagctgggga tggcccttat ctgaatgacc
    16081 tcaaagagca agcccagaac ctagagattc aagactcagt catctttaca gggatgattg
    16141 ctcctagtga gacggctctt tactataaag cggcggattt cttcatttcg gcatcgacaa
    16201 gcgaaacgca aggtttgacc tacttggaaa gcttagccag tggaacacct gtcattgctc
    16261 acggaaatcc ttatttgaac aacctcatca gtgataaaat gtttggagcc ttgtactatg
    16321 gagaacatga tttggctggt gctattttgg aagccctgat tgcaacacca gacatgaatg
    16381 agcatacctt atcagagaaa ttgtatgaga tttcagctga gaactttggg aaacgagtgc
    16441 atgagtttta tctggatgcc attatttcaa ataacttcca gaaagatttg gctaaagatg
    16501 atacggtcag tcagcgtatc tttaggacag ttttgtatct tccgcaacag gtggttgctg
    16561 tacctgtaaa aggatctaga cgcatgttga aggcttcaaa aacacagttg atcagtatga
    16621 gagactattg gaaagaccat gaagaataga aagaggaaca gctatgaaaa aaacaattaa
    16681 tgagaagcgg tcgtgataaa aagattgcgg gtgtttgtgc tggggtggcc cattatctgg
    16741 atatggatcc gactatcgtt caagtcattt ggggtgttct tacttgctgt tacggagctg
    16801 gaattgtagc ttacattatt ttatggatta tcgcaccagt agcaactgac tattgaaaac
    16861 tagctcaatt catgctaaga taatagaaaa tagaatgtaa cgaaggagag aaaaatggca
    16921 tttggagata atggaaatcg taaaaaaact atgtttgaga aaataacctt gtttatcgtg
    16981 attatcatgc tagtagcaag tttattggga atttttgcaa ctgcaattgg tgccctcagt
    17041 aatctataaa atagattcaa gaaaatttag tgactgggat ttcccagccc ttttttaaag
    17101 tgagaagaaa taatgagtat gtttttagat acagctaaga ttaaggtcaa ggctggtaat
    17161 ggtggcgatg gtatggttgc ctttcgtcgt gaaaaatatg tccctaatgg aggcccttgg
    17221 ggtggtgatg gtggtcgtgg aggcaatgtg gtcttcgttg tagacgaagg actacgtacc
    17281 ttgatggatt tccgctacaa tcgtcatttc aaggctgatt ctggtgaaaa agggatgacc
    17341 aaagggatgc atggtcgtgg tgctgaggac cttagagttc gagtaccaca aggtacgact
    17401 gttcgtgatg cggagactgg caaggtttta acagatttga ttgaacatgg gcaagaattt
    17461 atcgttgccc acggtggtcg tggtggacgt ggaaatattc gtttcgcgac accaaaaaat
    17521 cctgcaccgg aaatctctga aaatggagaa ccaggtcagg aacgtgagtt acaattggaa
    17581 ctaaaaatct tggcagatgt cggtttagta ggattcccat ctgtagggaa gtcaacactt
    17641 ttaagtgtta ttacctcagc taagcctaaa attggtgcct accactttac cactattgta
    17701 ccaaatttag gtatggttcg cacccaatca ggtgaatcct ttgcagtagc cgacttgcca
    17761 ggtttgattg aaggggctag tcaaggtgtt ggtttgggaa ctcagtttct ccgtcacatc
    17821 gagcgtacac gtgttatcct tcacatcatt gatatgtcag ctagcgaagg ccgtgatcca
    17881 tatgaggatt acctagctat caataaagag ctggagtctt acaatcttcg cctcatggag
    17941 cgtccacaga ttattgtagc taataagatg gacatgcctg agagtcagga aaatcttgaa
    18001 gaatttaaga aaaaattgtc tgaaaattat gatgaatttg aagagttacc agctatcttc
    18061 ccaatttctg gattgaccaa gcaaggtctg gcaacacttt tagatgctac agctgaattg
    18121 ttagacaaga caccagaatt tttgctctac gacgagtccg atatggaaga agaagcttac
    18181 tatggatttg acgaagaaga aaaagccttt gaaattagtc gtgatgacga tgcgacatgg
    18241 gtactttctg gtgaaaaact catgaaactc tttaatatga ccaactttga tcgtgatgaa
    18301 tctgtcatga aatttgcccg tcagcttcgt ggtatggggg ttgatgaagc ccttcgtgcg
    18361 cgtggagcta aagatgggga tttggtccgc attggtaaat ttgagtttga atttgtagac
    18421 taggagactg gtatgggaga taaaccgata tctttccgag atgcggatgg taattttgtt
    18481 tccgccgcag acgtttggaa tgaaaagaaa ttggaagaac tatttaatcg tctcaatcca
    18541 aatcgtgcct tgagattggc acgaactaaa aaggaaaatc catctcagta aagaagctaa
    18601 aaaatcccgt gcctcatcag acacgggatt ttgtggtacg acaggcatgt atagcaaact
    18661 gaatctggaa tagcacagca tatcttctaa aatattgtta taaattgatt tgaatttcaa
    18721 aattaaattg tttgattctt atctcaattt gttatagtat atctgatgtc aaagttctcg
    18781 gcgagtcaaa tagcgattcc caagcctgac tatcgtgagg tagcggatta aaatggtctg
    18841 gggatagacc gttttaagtc tgacgctgga aataagaatt gtcagaagaa gggatagcga
    18901 aatcgtggct ctacgaacag gaacgtgata ataaggcgta tatagcggat aagagggcct
    18961 caaactctaa agtccaaaaa ggtagtcgta acctatatgc gtaaatcacg agagtaattg
    19021 aattcgtact aagattttct attttcactg taacctttta acgtccttat atcttgtata
    19081 cacgaggaaa gatgtacgac ttatcccgtg aggtctatca ctataaagag aaaacgacag
    19141 atagaagtga tcctgagtca cggttatctt taaaatagat tgtctgatag gacggtatgt
    19201 ataaaacgct tctgtgaact gagagaaggg ggagaagttc ttgctaaaat ttagttgaac
    19261 agccgtattc cgatacttag ataagagatc tagtcttagc tcctactcag ttttagggga
    19321 taaaaaaggg gaaatagcga ttcgagaaag attatactct tcgaaaatct cttcaaatca
    19381 cgtcaatatc gccttgccgt atgtgtagga tactgactac gt
//
LOCUS       DAVBCW010000030        18942 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVBCW010000030 DAVBCW010000000
VERSION     DAVBCW010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18942
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.030"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(146..397)
                     /locus_tag="VQ514_000906"
     CDS             complement(146..397)
                     /locus_tag="VQ514_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499683.1"
                     /translation="MPKTKTLAELADVILWSFDFANDHAHAFFMDNVEWSHADSYFLS
                     FVSDDVEERYIENVYLDSLSVKQKFKFIFDFGDEWRFEC"
     gene            complement(687..2567)
                     /locus_tag="VQ514_000907"
     CDS             complement(687..2567)
                     /locus_tag="VQ514_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HET2499684.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIQGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDGLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDIL
                     CPILDQHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(2561..3430)
                     /locus_tag="VQ514_000908"
     CDS             complement(2561..3430)
                     /locus_tag="VQ514_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2499685.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPDLENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNILCQEAIERMNRNLAQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQDVKKAVDNFVDTYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(3514..6159)
                     /locus_tag="VQ514_000909"
     CDS             complement(3514..6159)
                     /locus_tag="VQ514_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HET2499686.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDERFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGKQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTDRYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELIVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFALECHQAQERFSAFRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVFRYYNMSQENVHISEHQQTILDLLE
                     RPYPVHSGPLDPQEIRTEFIKKEEI"
     gene            complement(6250..7530)
                     /locus_tag="VQ514_000910"
     CDS             complement(6250..7530)
                     /locus_tag="VQ514_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HET2499687.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETSQFDETF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIENYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSIDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            7701..9785
                     /locus_tag="VQ514_000911"
     CDS             7701..9785
                     /locus_tag="VQ514_000911"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HET2499688.1"
                     /translation="MKPLLETIDTRFGTASKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDIDK
                     ACFQPHYLKLFSLRYQIETQLTPTCYGASIRLNQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKTSAQADWENLLHRFDIIEIGEADRTFFDHCLYRLFLFPQTF
                     YEINESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAT
                     SQNYRQLFDTETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKVCQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHSHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPNH"
     gene            complement(9825..11504)
                     /locus_tag="VQ514_000912"
     CDS             complement(9825..11504)
                     /locus_tag="VQ514_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HET2499689.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGACLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWASDADLPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGRGFTVGHKRLLRGPLVEAQKVRVMITEAQSMPLLTKISLYKT
                     PGLSKKEVVQELAFAEKSLAVAKGETLHFRIERSESSSSLEAKISIQPGTGVHGVAYR
                     DEIQVLQFQAGESKKDLHLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
     gene            12260..13489
                     /gene="arcA"
                     /locus_tag="VQ514_000913"
     CDS             12260..13489
                     /gene="arcA"
                     /locus_tag="VQ514_000913"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HET2499690.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            13547..14563
                     /gene="argF"
                     /locus_tag="VQ514_000914"
     CDS             13547..14563
                     /gene="argF"
                     /locus_tag="VQ514_000914"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HET2499691.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            14728..15675
                     /gene="arcC"
                     /locus_tag="VQ514_000915"
     CDS             14728..15675
                     /gene="arcC"
                     /locus_tag="VQ514_000915"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HET2499692.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            15884..17395
                     /locus_tag="VQ514_000916"
     CDS             15884..17395
                     /locus_tag="VQ514_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HET2499693.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYET
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            17417..18748
                     /locus_tag="VQ514_000917"
     CDS             17417..18748
                     /locus_tag="VQ514_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HET2499694.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKCVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     PLYEQVCNDLKEAGYDYQSTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
BASE COUNT         5323 a         4288 c         3909 g         5422 t
ORIGIN      
        1 tgaaatacta agcctacaca gactgatttc aattttacca ttcttcatag tcaaaaccat
       61 cataatctgg atattgttct ggcaacgttc caaccgaacg tacgagataa gcttcttcgt
      121 cctctgtctc gatttctctc agcacttagc attcaaaacg ccattcatca ccgaagtcga
      181 aaataaactt aaatttttgt ttgacactta ggctatccag atagacattt tctatgtaac
      241 gttcttcaac atcgtcacta acaaagctaa gaaagtaaga atctgcatga ctccactcaa
      301 cattatccat gaaaaatgca tgagcatgat catttgcaaa atcgaaactc cataaaatca
      361 catctgctag ttccgctaac gtttttgttt ttggaatcta tatatgtcgg tagcaacctt
      421 ttttaatttg acggaaatga tccaagcttc tacagatgct ggcttttcag cttctatttc
      481 ttgttttact aattttaact gtagcatttg aaaccagata attcttctgt ttcacgcgaa
      541 acaccctgcg ccaagcaagg tgttcttgtt tttatactca atgaaaatca aagagcaaac
      601 taggaagcta gctgcaggct gctcaaaaca ctgttttgag gttgtagatg gaagctgacg
      661 tggtttgaag agattttcga agagtattaa gtcgtataaa tcgtcgaagc tgtcgcaatg
      721 gtatgccact ggttagctgt agttgctgcg aaatccttgt ctgcgtagaa gtcagtaaat
      781 ggtaggattt caacttccag ctcgtcgatg cggtcaagct ggccagccag ataaacctcg
      841 atacggcttt ctgctcgttt gatgcgttgc aagagtccgc ccatacggat gtcaactgta
      901 tccaaaccaa agaccttgtt ttctttcagc cattggtgac taaagagggc atggaagtct
      961 tcaatttggc ttctaagttc tggtaattct tgtctggcga tttgttgtaa actttcttta
     1021 tcatccgctt ggtaggcctg acgaatgcgt cgtcccacat ctactttgct acttaaaata
     1081 gcattcaact gggcctgagt ttcaaagaga taagcatagt ttccagcttt ttctttaatg
     1141 ttagcaagcg tctcagcagc ctgagcgaag tgcggtttgt cctgttcagg tgtcatgtgt
     1201 tgatcaagaa tcggacaaag aatatcctga taaaaaacat agcggttggg attgataccg
     1261 ctgagattgc ctggtaggtc tggtaagagg ttggcaaggt caatctgcat aaaatcctca
     1321 accgttagac cagtattggt cttgaagtgc gcagacaaac catctaggtc attgcgatag
     1381 ctgagttctg cccagatttg caagcttggt aggatagaga actgggcagt ttcaccacca
     1441 ttgtctcccc aacccgttac gatgacttct ttaatctgat tggcacggca ggctttatta
     1501 gcctcgatag ccactagacg gctaaaatgg ttgtgaggtg taaagccaat ccacttccaa
     1561 gctccccctg caaatgcaag gtcatggcta atcttgtgat gattgcggaa attacggttg
     1621 tatttttcct cgctatcctg ataataatcc cagtaaacca gagtcacacg gtctttgaga
     1681 cggtctaggt agacacgagt ttcctctgga atttccacat cacggtcgta ctggccatcc
     1741 gctgacatga gtttgaagaa catatcactc cacatctggc agtggaaacc atatttgtca
     1801 gcaatatcca gcacgcgctc caagtgttgg cacatgagga gactacgatc cacaacaccg
     1861 ttcaaaatca ggtagcgtcc caaaccaacc aagtgggctt cgtccatccc gatattgacc
     1921 ttgcgagtct tcagtttaga caacgtggca aacatgccat caatcaggtc ataaaccttt
     1981 tcttcgccaa tgagaagaat gtcctctaca tcacggagct cctgcacttc cttgacaccc
     2041 catttgacaa aggccgacaa gtgggccaag gtctggatgc atggtacaaa ggtcatgtca
     2101 aactgttgag cataggcttc gatttcctgc aactcctctg ctgaataagc tccacggaaa
     2161 tagccaaagt aaggctgccc ttcaatctgg taagtgtctt ccatgtaaag ttcaaaggtt
     2221 gagtagccca tgagagccaa gacctcaatc atctgcttgg cagaagccac attcagcacc
     2281 gcatttcgag aacagtcaac catgtaagcc aaatcttcgt aagctgcttg ttcctcaatc
     2341 tctactttat cagcttctgc tagaactgtt accaacaagg ataaggcacg ataaagttgg
     2401 tgaggtttgc ggtaggtcaa ttgatagtga ccttcctcac cctggataga gatagaggct
     2461 tggtcagact gagtgacagc cacttccaca tctggtagag aaatatgacc ttttaaaacc
     2521 tcaatagctt gcgtttgttt gggactaagt cctgtaaatc ttaccattgc ttttcctcct
     2581 gtagccagtt gacaagagca ccgtagagat tggcatctgc gtgataggtg caggcctgga
     2641 taactggtgc gaccgtgtat tcttcgtagg tatcgacaaa gttatcaaca gccttcttga
     2701 catcttggat aaaatctgga ttttgactga tagagccacc cagactgatg acatctggat
     2761 cgatcagata ctggatattg agcaagcctt gcgccagatt gcggttcatg cgctcaatgg
     2821 cttcttgaca aaggatatta ccagctgcgg cctcttggta aatcttgcga ccgtcccaat
     2881 cagtatgacc agatttttca atcacgtatc gtaccatatt cccagttgat gctagttgcg
     2941 accagttatt aagtttttca gcaggggcaa gggttgtcat gtagccaaat tctccaccca
     3001 agccgtggcg acctcgatga agtctaccat tgataatcat ggctccgcca atccctgtcc
     3061 caatcacgac acaggctgca ttttcaagat ctggatgagc tagtagttca ctgagtccaa
     3121 cgcagttggc atcattttct aaatggacag gtagctgata agagctaagc gcctcatacc
     3181 aagaaaagcc atggatgtag ggcaccgcac tgaagccatc aatcacacct gtctcttgat
     3241 tgactgcacc tggaacgctc atagcaatcc cactgtaatc ctgttctgac aagcgttgat
     3301 ctagccacgc tagtaaatcc tccaagtttt caggcgttga aatacttgtc ttatccagta
     3361 ttttcccatc aggagtcaga ctggcaaact taatcccagt ccctccgata tcaatcgttg
     3421 caatggtcat tgtttttacc ataaaaaggg gcgaatcagc agttagttct tactttttcg
     3481 cccctccttt cttttgatgt ttactttttg aaattaaatt tcttcttttt tgatgaattc
     3541 tgtacgaatc tcttgtggat ccaatggtcc tgaatgaact ggatatggtc gctcaagtaa
     3601 gtcaagaatg gtttgttgat gttctgatat gtgcacattt tcttgactca tattatagta
     3661 acggaagaca tagccttctt cattttccgc cactttaaag gctgttggac aaacttgcgg
     3721 tatgctgaga acagaatggc tcaagaggct accagtcgca accacgcttc cttcctgtct
     3781 agcaagctga aggctggtaa acggtgtctg caaggctttg gcacgacgga aagctgaaaa
     3841 gcgttcttgg gcttggtggc attcaagtgc aaactcgact tcaaactcac gcaagcattg
     3901 tgcttctggc gttgggaagt agccccagtc acctagctca cctgatgcac gcaaaatggt
     3961 cacggcaatg gtgttatccc caaggatttc gtattcattc aatcccttat tggatacagt
     4021 cacccctttt tcatcgtcat acagactgac aaaagcttgt tggtgttgag gattttcagg
     4081 attttcccat gaagcagctg gtttgtttgg tcgtgtcacc acctcataga tactttcaga
     4141 atcattgctt ggacgcgtgt tatgagtctt gaccaagaga cggatacggt gatccttggc
     4201 agtgttagta aagcgagtct tgaagcggat ttgtggattg tcaacaaaga caatcaactc
     4261 agtttccaga ggaatgtttg tcaattcttc tgaccgtcca gcctcacgct tcataaactc
     4321 gatgatacct tgttgctctt cttctagctt ttcatccgca ctgacaggca cggtcaattc
     4381 atgtttgagc aagattttag catagcgatc tgtgttttcc aagacctcgt ggcccttaag
     4441 ctctgcaaag attggctctg ttccttttgg ttggaaatag atatactcgt ttccgatgtc
     4501 cccacggtct tcgaagcgga taaagtcttc ataggcttcg tgagttgtct tgtcatatac
     4561 tgtgatattg tcatccacac tcaccgttac gaatggtgta tcaatcactc cgttttggta
     4621 aataccctca cggtgttctt gttttccttc cagcaattgg aaggttgtcc aagaaagcgg
     4681 cgctaggtga actggaatgg tcacgcgcac ttgtcgagca atacgagctt ggcggaactt
     4741 gtcttttggt aaatcgtact caaaattagc tccgaggtct tcgattgtag cctctacagg
     4801 acgaccatcc aagtcctcca cacggtaact tggtaagata agagcagcca tctttttgta
     4861 gccttctgtt gggtgcaatt ccttgaaatc acaagtcgcc acatcaatca ctgtgctgac
     4921 agtatcgacc ttatcatgca agcctgtgtt aatgacagta aagagatagt cactttgagc
     4981 cttatccgta gcaattttac ccttccactc gttgagcaag ttacttttaa caaagtttcc
     5041 tacttggttg accttggcaa aacgcgtttc catctcgcgg tgaacttcgt ccacgctaca
     5101 gccacagata ctatcatgtg gcgcattctg caaaagtgtt ttccaagcat aggtcaactg
     5161 gtccttgtgg ttgtgtccac cagtgataat agtcaagggt tctaccactt gctctaggag
     5221 gttgctattt tcttggaagg cttgttttag gtaaatgcgg gatgaagaag tgttggcaag
     5281 tgtgtaccag ccatctgttt cctgactggt caactcgcct gtaacagttg ataagtgttc
     5341 aggaagcgca ccttctacag cttgaacata ttcatcaaaa gaactatgaa caaagattac
     5401 atccgggaag agttcatttg ccacacgaat ggcttcgctc agatttttct gtacaggctg
     5461 gtggtcacag ccgttcatca tcaaccattg gttggtcgaa gcgtaggcac gcacatctga
     5521 caatttttgt ttccagaagg tcaaggcctc atctttgtca actgggattt cattcccatt
     5581 actgtaccag ttggcaaaga gaatacctaa aacacgacta ccatccacac cctgccagta
     5641 catttctgaa aactgggatg taaaccgctc atcttcaagg acttggttgt caaatccaat
     5701 cggcttcaca ccacgaccaa aggctgccac gtgaatacct gatttttgaa ggatttgagg
     5761 agcttgtccc atatttccaa aggtatctgg aaagtagcca atctgggttg atttacccca
     5821 tttggcagct tcttgttgac caatcaaggt attgcggaca ttggcttcac tggagatcaa
     5881 gtagtcatcc tgcaagatgt aaaagggacc aattttaagt ttgccctcgt caatgtagcg
     5941 ttggaccttg tcgcgatttt cagggcgaat ttgtaagtag tcatcaagga caatagtttg
     6001 tccatccaag tggaaactct tgaactcagg gtcattttca aagagatcaa agagattgtc
     6061 aaacaattcc accaactgca tacgatggct ttcaaaaggc aagtaccact cacgatccca
     6121 gtgactatgt gagataatat gtacaacaac attttccatg aagtaaaacc tcattctaaa
     6181 tttaaatttt aacgttttaa atgtaaactt gtgattctga taagaatcgg ttgagctaaa
     6241 gcgagcccct tagcggatat ccaagtaatc caagaccaac tcacagaaca tcatgttagc
     6301 ccaggagaac cattcacgag agtagagggt cggatcatct acgtggaagc tttcgtgcat
     6361 gacacctgta ccaccatcgc aggcaaccag ctgatccagc aagaattttt tctctgcctt
     6421 atctcttgtt gtcaatcctt ggatagaaag ggcgattggc cagatatagc gatagaaggt
     6481 atgagaactt ccgagaccgc tagcgtattc tccttggtag aagtatggat tttcagagct
     6541 cagaatggta cggcgagtgg cttgatacac ttcatcatcg atcgaacagt agcccagata
     6601 gggcgcagct agtagacttg gtacatttgg atcatccatg atgctggcat ttcctaggcc
     6661 atctacttca aaagcgtaaa tcttttcgcc cttgctgttg gtggtgtaag cgtagttttc
     6721 gattccttct tggatttcat cctgaagacg cttggcatca gcaataacac tctggctatc
     6781 agctaggttt aatgctgcga agatttcttg cacataaccc aagactacta cagcaaacat
     6841 atttgacgga atcaagtaac tatactggca acagtcatca ctcggacgaa aagctgacca
     6901 agtcatacct gtcactgcaa agtcaggtcc aaagccatca tttaccaagg tgtcttcctt
     6961 acggtccgta tctcggacaa aacgataagg agagttcttg tggtcttgtt caactgtcca
     7021 tagatggaga atttccttag tcgctgcgac aaaagtttca tcaaactgac tagtctcgcc
     7081 agtctctttc cagaggagat aagccaactg caaaggatag caaagcgaat ccacctcata
     7141 cttgcgctcc cagatccagc cgttaaggtc tgtgtggtca gtctcgtggt gccctttcca
     7201 gttctcctca atgttgaagg agttagcata gggatccttg agtaccaagg tcatctgacg
     7261 tttgaccaaa cctgcaatgg tctgacgcag gagggcatct cttttagcta catgaaggta
     7321 gggtctgagt tgggctgtcg aatctcgaag ccacatggca ggaatatccc cagtcaagac
     7381 aaaagttgaa ccatcttcta agatttcaac cgtattgtcc aaggtatcgg tgtagcaacg
     7441 ctcaaagaca tcaacccact ctggatggtc cttggcccgc tctgctactt catctagcca
     7501 ttctctaaca atttctttcg aataaatcat cgtgcaattc cctctttcaa acaagtttca
     7561 tgttcagtat atagcttttg agacagaaaa tacatacaca aatgatagtc atttttctac
     7621 aaaatagtta tctttaaaac tccttttcta aaggccctct ttttctgata taatgtagaa
     7681 aaacagctaa aggaaagact atgaaaccac tacttgaaac catcgatacc cgctttggta
     7741 ctgctagcaa gcatgccttc tctcggggaa ataccctgcc atacacaggc gtgccttttg
     7801 ggatgaatta ctttgtgccc cagaccagtg accaggatgg atcttggttt tttgatccgc
     7861 atctgcctat ctttcagggg attcgattaa ctcaccagcc cagtccttgg attggggact
     7921 actcttggct ccttctgaca cctgtcacca gtcaactggg gggagacagc ctctttcacc
     7981 gccagtcttc ttatgatata gataaagctt gcttccagcc tcactatttg aagctctttt
     8041 ctctgcgtta tcagattgaa acccagctca caccgacttg ctatggtgct tctattcgtt
     8101 tgaatcaaaa gcaaggcaaa gccctctccc tctatcttca cgcagcagat gaactgacag
     8161 tagagcaagt agataagcgg actcttgccc tgcgacaaga aggtaaaact gaaactaaca
     8221 aaaattcgct aacgatgttc actgccctgc aaatgaacac ggatattctt gctatcagcc
     8281 aagaagctgg ggactggcga attgacttag caagtagtca aaccgagatg caactagcca
     8341 cttctttcat ctctccttct caagctctaa tcaatctacc tcaagaagat tttgatagct
     8401 gtaaaacaag tgcccaagcg gattgggaaa atctcctcca tcgttttgac attatagaga
     8461 taggagaggc tgaccgaacc ttctttgacc actgcctcta cagactcttc ctcttcccac
     8521 agacttttta tgagattaat gaatcagggc aagccatcca catggatctg gctactggta
     8581 ctgtcaagcc tggtgtcctc tttagcaaca atggtttctg ggataccttc cgcaccacct
     8641 tccccctctt tgcccttatc ataccggagc actatcaacg ctttttagaa ggtttcctca
     8701 atagctaccg cgatactggt ttccttccaa aatggctggc tccagatgaa cggggtatga
     8761 tgccaggtac acttttagac ggtattatcg cagatagcgc ctgcaaggac atggcccctg
     8821 acctagaagg agaactcttc caagccatgc tcgaaacagc cagcaaagcc gaccctctcg
     8881 gcatcaatgg ccgccacgga ctagcccaat accaagaact aggttacctc tctaccgacc
     8941 accacgaaag tgttagccat actctagact atgcctatag tgacttttgt atcgccagct
     9001 gtgccaaaaa actagagaac atagaaatcg ctgaaaccta caaggctact tcacaaaatt
     9061 accgccagct atttgacact gagacaggtt acatgcgagc acgagataat caaggaaact
     9121 ttcaccctga cttctctcct tatagttggg ggcgagacta cgctgaatgc tctgccattc
     9181 aagctacttt aggcgttctc cacgacatcc ctggcttaat ccaactgatg ggtggaaaag
     9241 aaacctttag caactatctt ttgaaagtct gtcaagatgc tcccctcttt gaaacaacag
     9301 gctatggtta cgaaattcac gaaatgagcg agatggctac tgctcctttt ggacaaatag
     9361 ccatttccaa ccagcctagt ttccacattc cttatctctt ccgctacagc gattaccctg
     9421 actacactgc ccttcttatc aagacgctcc gtcagaaagc ttttcaccca agttgggaag
     9481 cctatcctgg agatgaagac aatggtagtc tctctgcttg gtacatctgg tcagctctcg
     9541 gattttatcc aacctgtcca ggaaaaccaa gctatgacct cggaatccct ctctttgacc
     9601 atctacgagt ctacctagct aaagaagata aatggctgga tatccatact aaacaaaacc
     9661 acagccattt taactttgtc aaagaatgcc gactggacaa aacactcgta tcaactattc
     9721 aacaccaaga cctcctaaaa gctgaacaac taactttcac cctcagctgg ctaccaaatc
     9781 actaataacc aaaagaacct ttgcatcgcg caaaggttct tcttttatga aacttggact
     9841 tgaagttgat ccacaagctg accatccacc gttaggttca gatagaaatc caaggtttta
     9901 tctcctgcga aatacagggt tggtagatgc aaatcttttt tgctttcccc tgcttggaat
     9961 tgaaggactt gaatctcatc ccgataggcg acaccatgga cacctgtccc cggttgaatc
    10021 gaaatcttag cctctaaaga ggagctactt tcactacgtt caatcctaaa atgaagagtc
    10081 tctccctttg ccacagctag gcttttttct gcaaatgcta gttcctgaac aacttctttt
    10141 tttgataacc caggagtttt ataaagggaa atcttggtca gtagaggcat agattgtgct
    10201 tcggtaatca tcacgcgcac cttctgtgcc tctactagcg gacctcgcaa gaggcgctta
    10261 tgaccaactg tgaagcccct gccaaattcc tgccagacac catccacctc tacttgcaca
    10321 tggaaagcag cgattcgttg ccctagcttc aaatcttctc ttaactcaat tacatcaaaa
    10381 gttttaggag aacctaagtc gagttctaac tggatgggca agtctgcatc gcttgcccaa
    10441 gagctggtct caaggccgtc tgtcaaatgg cgacaagcaa agtctgcgga aagagctgga
    10501 ccagatacct cagctcccag agccaaatct tctttataga gctcattgcg ataggtcgca
    10561 aattcataaa gtcgttcaat atcctttgca tcaaagagcc cagcttgatt cggcggaata
    10621 ttaagcaaga gtggagttcc tcgccctact gagtgaaagt agatttcgac caactcctcg
    10681 agagacttag gatcctgatc ctcatggtag aaccagcctg gacggatgga aacatctgcc
    10741 tctccgattg aaaaaatcgt gcccgaggga tccccgtgct gaagatagtt cagctctgct
    10801 tctgttccta gtttatcagg attcaccttt tgccacagtg gatcacctgc ataccctcgt
    10861 tcattgccaa tccagcggat actggtgcct tctgttgaaa aaatcaagca agcgccctgc
    10921 aggtcacgaa tggtttcaaa ccatttttca aattcataat taaccttttg cgcgccctct
    10981 cctctggcac catccatcca aacctcagcg aacttaccag cattcccata gttaggattt
    11041 gataagattt ccttcaactg agccagataa taggcattgt agtccgcttc tcggtccaca
    11101 tgatagaggg gactatgggc atcccacggt gacaggtaga cccccatatc catatcaaac
    11161 tctgtggcag cttgggatac ttcaaggagc aagtcgccct ttcctctcct ccaaggactg
    11221 accttaaccg aataatctgt gtgagctgtc ggataaagga caaagccatc gtggtgcttg
    11281 accaccaaaa tcaacttttt gaagcccgtt tccttgagca cacgaaccca ctcacgcgca
    11341 tccaactgac tcgggttaaa gcgctcagga tcctcctgtc cagtccccca ttcttggtca
    11401 taaaaggtat taggaccgaa gtggataaaa gctgctagtt catctcccag ataagctagc
    11461 tgagtctgac ttggtaacgg tccatgcggt ttgattttct tcatcgttat atatctcctt
    11521 ctttagcaac ttctcttgtt cacaaggtta tccacaggtt gtggataata aaccatgatc
    11581 taacagccag ctctttccaa ctccaactgg taggctcagg atttttctcc tcaatattcc
    11641 ctcttcaaac agctctgtca tatggttctg ctaacacctc tcagaatgct actctccata
    11701 attattctca ggcttctttc aggtcattgt gtgaagacca ttcactctcc agacttttcc
    11761 acagattgtg aataagtgat tattctaaac tatcacctcg tcgactataa caaaaaataa
    11821 aaagagggtc aataagataa ttatctcttc ctaaatactc agaaaagatt ctataatttg
    11881 tacaattgaa caagaaatgc ttctccaaaa tctatcttca aaagaatgtt gcatcttttt
    11941 atcctttcac acaaacattt ctctttatac agatccaagt gataaccaga tgataattgg
    12001 acactttttg gttatttgtg tattttttga aaaatttttt aaaaaaattt tatatcctct
    12061 ttaatagaag tttatgcatt tgtatatatg tattccatcc tatgttgtgt ataataacat
    12121 aaaattgtgc taaatatcat aatttatatt attttccgag aaattatagc cattgacatt
    12181 acctaggaat tgcgttataa catacatgta agcggtaccc aaaatggtgc caagtcaaaa
    12241 tttttaagga ggaaaataca tgtcttcaca tccaattcag gtcttctcag aaattgggaa
    12301 actgaaaaaa gttatgttgc accgtccagg caaggagtta gaaaacttgt tgccggacta
    12361 tcttgaaagg cttctttttg atgatattcc tttcttggaa gatgctcaaa aagaacatga
    12421 tgcatttgcc caagctcttc gcgatgaagg aattgaggtt ctctacctag aacaactcgc
    12481 tgctgaatca ttgacctctc cagaaatccg cgatcaattt atcgaggaat acttagacga
    12541 agccaacatc cgtgatcgtc aaaccaaggt tgctattcgt gaattgcttc acggcatcaa
    12601 ggacaaccaa gaattggttg aaaaaacaat ggctgggatt caaaaagttg aattgccaga
    12661 aattcctgac gaagctaaag atctaactga cttagttgaa tcagattatc catttgcaat
    12721 tgacccgatg ccaaacctct atttcactcg cgacccattt gcaacaattg gaaacgccgt
    12781 atcgcttaac cacatgtttg cagacactcg taaccgtgaa acactctacg gtaagtatat
    12841 cttcaaatac cacccaatct atggcggaaa agtggatttg gtctacaacc gtgaagaaga
    12901 tacgcgtatc gaaggtggag acgagctagt tctttctaaa gacgtccttg cagtaggtat
    12961 ctctcaacgt acagacgcag cttctatcga aaaacttttg gtcaacatct tcaagaaaaa
    13021 tgttggcttc aagaaagttt tggcctttga atttgctaac aaccgtaaat tcatgcactt
    13081 ggatactgtc ttcactatgg tagactatga caagttcact attcacccag aaatcgaagg
    13141 cgaccttcac gtttactcag ttacttacga aaacgaaaaa cttaaaatcg ttgaagagaa
    13201 aggtgactta gctgaacttc ttgctcaaaa ccttggtgta gaaaaagttc atttgattcg
    13261 ttgcggtggt ggcaatatcg tagcagctgc gcgtgaacaa tggaacgacg gttctaacac
    13321 tttgaccatc gcacctggtg tggtagttgt ttatgaccgc aataccgtga ccaataagat
    13381 tttggaagaa tacgggcttc gcttgattaa gattcgcgga agtgaattgg ttcggggccg
    13441 tggtggacct cgttgtatgt ctatgccatt tgaacgtgaa gaagtgtaat cgctgttcgg
    13501 cattcgtcaa tagaaaatgt aaaaaataga aagaggaaat aataaaatga caaattcagt
    13561 attccaagga cgcagcttct tagcagaaaa agactttacc cgtgcagagt tagaatacct
    13621 tattggtctt tcagctcact tgaaagattt gaaaaaacgc aatattcaac accactacct
    13681 tgctggcaag aatatcgctc tcctatttga aaaaacatct actcgtactc gtgcagcctt
    13741 tacaactgcg gctatcgacc ttggtgctca cccagaatac ctcggagcaa atgatattca
    13801 gttgggtaaa aaagaatcta ctgaagatac tgctaaagta ttgggacgta tgtttgacgg
    13861 gattgaattc cgcggattca gccaacgtat ggttgaagaa ttggcagaat tctcaggcgt
    13921 tccagtatgg aacggtctaa ctgacgaatg gcacccaact caaatgctcg ctgactactt
    13981 gactgttcaa gaaaacttcg gtcgcttgga aggcttgaca ttggtatact gtggtgatgg
    14041 acgtaacaac gttgccaaca gcttgctcgt aacaggtgct atccttggtg tcaatgttca
    14101 catcttctca ccaaaagaac tcttcccaga aaaagaaatc gttgaattgg cagaaggatt
    14161 tgctaaagaa agtggcgcac atgttctcat cactgaagat gctgatgaag cagttaaaga
    14221 tgcagacgtt ctttacacag acgtttgggt atcaatgggt gaagaagaca aattcgcaga
    14281 acgtgtagct cttcttaaac cttaccaagt caatatggac ttagttaaaa aagcaggcaa
    14341 tgaaaacttg atcttcctac actgcttgcc agcattccac gatactcaca ctgtttatgg
    14401 taaagacgtt gctgaaaaat ttggtgtaga agaaatggaa gtaacagacg aagtcttccg
    14461 cagcaagtac gctcgccact tcgatcaagc agaaaaccgt atgcacacta tcaaagctgt
    14521 tatggctgct acacttggta acctttatat tcctaaagta taattttaga taataaaccg
    14581 tctaccaaca gctatgaggg ctgcgactaa tagctttagt ccggtcctct tttatgtaat
    14641 ggtaatctat tatttcttat aaaatatgtg aaaaatcatt aaattgaaat ctaaacgcat
    14701 tctattgagt gtgataaagg agaatttatg gcaaatcgta aaattgtagt agctttggga
    14761 ggaaatgcga ttctttcttc tgacccatca gcaaaggctc aacaagaagc tttagttgaa
    14821 acagctaagc atcttgtaaa attgataaaa aatggagatg atctgattat cactcacggt
    14881 aatggacctc aagttgggaa tctcttgctc caacatttgg catcagactc tgaaaagaac
    14941 cctgccttcc cactcgactc acttgtcgct atgacagaag gtagcatcgg tttctggttg
    15001 aaaaatgctt tgcaaaatgc tctcttggat gaaggcatcg aaaaaaatgt tgcctctgtt
    15061 gtaacgcaag ttgtcgtaga taaaaatgat ccagcttttg ttaacttgag taaaccaatc
    15121 ggtcctttct attcagaaga agaagcaaaa gcagaagccg aaaagagcgg agcgactttc
    15181 aaggaagatg ctggccgtgg ctggcgtaag gtcgttgcct caccaaaacc tgttgacatc
    15241 aaagaaattg aaaccatccg tactctttta aataatggtc aagtcgtcgt agctgcaggt
    15301 ggtggcggta ttcccgtcgt caaagaaaac aatggacatt tgactggtgt cgaagcggtt
    15361 attgataaag acttcgcttc ccaacgtttg gcagaattgg ttgatgcaga cctcttcatc
    15421 gttttgacag gtgtagatta tgtatttgtt aactacaaca agccaaacca ggaaaaattg
    15481 gaacatgtga atgttgccca gctagaagaa tatatcaaac aagatcagtt tgcaccaggt
    15541 agcatgcttc caaaagtaga agcagctatc gcttttgtca atggtcgtcc agaaggaaaa
    15601 gcagttatta cttcccttga aaatctaggc gccttgattg aatctgaaag cggaacaatt
    15661 attgaaaaag gataagttgt tttactaata agatgtattc tatttctagt atctttatat
    15721 caaattagaa attattcttg aaaacatgta caatatttca aaagatacta gttttagact
    15781 ttaatatggt aaaacaaata taaatagaaa gcgttttctt aaatgtttat ttaagaaagt
    15841 agttggtttt ttacactttg ttagacatca ggaggaaaaa caaatgagtg aaaaagctaa
    15901 aaaagggttt aagatgcctt catcttacac cgtattattg ataatcattg ctattatggc
    15961 agtgctaact tggtttatcc ctgcgggggc ctttatagaa ggtatttacg agactcagcc
    16021 tcaaaatcca caagggattt gggatgtcct catggcaccg attcgggcta tgctaggtac
    16081 tcatccagag gaaggttcgc tcattaaaga aacgagcgca gcgattgatg tagccttctt
    16141 catccttatg gttggtggtt tccttggcat tgtcaacaaa actggtgctc ttgacgtagg
    16201 gattgcctct atcgtgaaga agtataaggg ccgcgaaaaa atgttaattt tggtactgat
    16261 gcctttgttt gccctcggtg gtacaactta tggtatgggt gaagaaacaa tggccttcta
    16321 tccactcctt gtgccagtta tgatggccgt tggttttgat agcctgactg gtgttgcaat
    16381 tattttgctc ggttctcaaa tcggctgttt ggcatctact ctgaatccat ttgcgacagg
    16441 tattgcttca gcgactgcgg gagttggtac aggggacggt atcgtacttc gtctgatctt
    16501 ctgggttacc ttgactgctc ttagtacttg gtttgtttac cgttatgcgg ataagattca
    16561 aaaagatccg actaagtcac tggtttatag tactcgcaaa gaagatttga aacactttaa
    16621 cgtagaagaa tcttcatctg tagaatctac acttagcagc aaacaaaaat cagttctctt
    16681 cttatttgtg ttgacattca tcttgatggt attgagcttc attccatgga cagaccttgg
    16741 cgttaccatt tttgatgact ttaatgcttg gttgactggt cttccagtta ttggtaatat
    16801 tgtcggttca tctacttctg cactaggtac ttggtacttc ccagaaggcg caatgctctt
    16861 tgcctttatg ggtatcctga ttggtgttat ttatggtctt aaagaagata agattatctc
    16921 ttccttcatg aatggtgctg ctgacttgct cagtgttgcc ttgatcgtag cgattgctcg
    16981 tggtattcaa gttatcatga acgacggtat gattaccgat acaatcctca actggggtaa
    17041 agaaggcttg agcggtctat cttcacaagt ctttatcgtt gtaacttata tcttctatct
    17101 acctatgtca ttcttgatcc catcttcatc tggtcttgcc agcgcaacta tgggtatcat
    17161 ggctccactt ggagaatttg taaatgtccg tcctagcttg attatcactg cttaccaatc
    17221 tgcttcaggt gtcttgaact tgattgcacc aacatctggt attgtgatgg gagctcttgc
    17281 acttggacgt atcaacattg gtacttggtg gaaattcatg ggcaaactcg tagtcgctat
    17341 tattgtagtg accatcgccc ttcttctcct tggaaccttc cttccattcc tataaaatag
    17401 tgagtgaggt gattccatga aaatagatat aacaaatcaa gttaaagatg aatttcttat
    17461 atcattaaaa accttgattt cctatccttc agtactcaat gaaggagaaa atggaacacc
    17521 ttttggacaa gcaatccaag atgtcctaga aaaaacttta gagatttgtc gagacatagg
    17581 tttcactacc tatcttgacc ctaaaggtta ttacggatat gcagaaatcg gtcagggagc
    17641 agagcttctg gccattctct gtcatttgga tgttgttcca tcaggtgatg aagcagattg
    17701 gcagacaccg ccatttgaag caactatcaa agacggctgg gtattcggac gtggtgtcca
    17761 agatgataaa ggcccttcgc tcgcagctct ctatgcagta aaaagcttgc tggaccaagg
    17821 tattcagttc aaaaagtgcg tacgctttat ctttggtacc gatgaggaaa ccctctggcg
    17881 ctgcatggca cgctacaata ccatcgaaga acaggccagt atgggctttg cacctgactc
    17941 atcttttcct ctgacctatg ctgaaaaagg gcttctacaa gtcaaacttc atggccctgg
    18001 atcagatcaa ctagagcttg aagtaggagg cgcctttaac gttgtaccag acaaggccaa
    18061 ctaccaaggt cccctctatg aacaggtttg taacgatctc aaagaagctg gttatgatta
    18121 ccaatccact gaacaaaccg taacggttct cggagtgcca aagcatgcta aggatgctag
    18181 tcaaggtatc aatgctgtca tccgactagc taccattctt gctcctctcc aagaacaccc
    18241 tgctctcagc tttcttgcaa cacaagcagg tcaagacggc acaggaagac aaatctttgg
    18301 tgatatagca gatgaacctt ctggtcacct atcctttaat gtcgcaggtc tcatgatcaa
    18361 tcatgaacgt tctgaaatcc gtattgacat tcggactcct gtcttagctg ataaggaaga
    18421 actagtagag ttgcttacaa gatgtgcaca aaactaccaa ctccgctacg aagagtttga
    18481 ctatctagcg cctctatacg tcgcaaaaga cagtaaactc gttagcacac tgatgcaaat
    18541 ctaccaagaa aagactggcg ataacagtcc tgctatttca tccggtggtg ccacttttgc
    18601 tcgcaccatg ccaaattgtg tagccttcgg cgccttattc ccaggagcga agcagacaga
    18661 acatcaggca aatgaatgtg ccgttctaga agatttgtac cgtgctatgg atatttatgc
    18721 cgaagccgtc tatcgacttg caacttaatc aggcaactgt ttctaccaaa gaaaatcgac
    18781 cgattaatga actgcacccc aaaagttaga cagaataaat ctaacttttg gggtgtttta
    18841 ttatgaaatt gagttatgaa gataaagttc agatctatga actaagaaag caaggacaaa
    18901 gcttcaaaca gctttcaaaa agatttgtgt gggtgtttcg at
//
LOCUS       DAVBCW010000031        18816 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVBCW010000031 DAVBCW010000000
VERSION     DAVBCW010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18816
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.031"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            <1..370
                     /locus_tag="VQ514_000918"
     CDS             <1..370
                     /locus_tag="VQ514_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET2499695.1"
                     /translation="CLVLVSIDYINCLSETADKNWHKSDRIFVTNTGKTVHSSILSKS
                     LQRANERLKKPIPKHLSPHIFIHITISILSENKIPLKTITDRVGHPDSEVTNSIYTHV
                     TKNMKDEAINVLDKVMKKIF"
     gene            complement(862..1272)
                     /locus_tag="VQ514_000919"
     CDS             complement(862..1272)
                     /locus_tag="VQ514_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499696.1"
                     /translation="MPKKKIERISVIHREKILWLKWYFMRDKEKPKYSVLECKMFDAA
                     KNKDMLAYKKYATIKQITDIRVQTSEDDILTAIKEVYVYNHMNVIGACQRILFVSQSP
                     AYNKLNKWFETYSDLYFSIIPLPNMGAYHELVDS"
     gene            complement(1290..1667)
                     /locus_tag="VQ514_000920"
     CDS             complement(1290..1667)
                     /locus_tag="VQ514_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499697.1"
                     /translation="MRKYLKEIKELQELKELLSSRNTPEVIIVEGNDDLGEFFQVDGE
                     LFSDIELLENLKKWREWEVQVIVDDWCNRGLNEYETGILYFPKHEDKMDYIRFNKGLE
                     PLYHALDEPYTTISKSEWLKLLD"
     gene            complement(1609..2037)
                     /locus_tag="VQ514_000921"
     CDS             complement(1609..2037)
                     /locus_tag="VQ514_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HET2499698.1"
                     /translation="MAGNENDGLTSKQIKFIDAMLTEPTIDKACQKAGVSRATGHKYL
                     KVAAVKKTLRLKQDEMMDKTTQMLYLASSNAVSVLNDIMMDAKINPFIRTQAAKTILE
                     QSYKTHEIFGVVRQIEELRLEIEEVSKGDQRVTRTQGIIK"
     gene            complement(2081..2686)
                     /locus_tag="VQ514_000922"
     CDS             complement(2081..2686)
                     /locus_tag="VQ514_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499699.1"
                     /translation="MTFTTIKDDIKAFGKKKHGYMTGYIAKQEELHKQLQNGMIGEKY
                     AKDQLEAYKLEGDTYSRDTYNKIHAEIEKQHDLELEALKEKELSVTADDVAELTLLAS
                     MKMTKDELLGYFEKYKNKPLAIKKLWSIAEQYPEIAINLELFNAEQALESLILFFKRQ
                     LSYCHYSLLINGDKIQAVTTEMVVNSDAPELDRRLDEYLNK"
     gene            complement(2776..2949)
                     /locus_tag="VQ514_000923"
     CDS             complement(2776..2949)
                     /locus_tag="VQ514_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499700.1"
                     /translation="MTENKDNKLLEMMEKGFVLYSKNGIIKYIEIPDHGSIKLKAQDG
                     QLVYKEVTTGEQC"
     gene            complement(3548..4444)
                     /locus_tag="VQ514_000924"
     CDS             complement(3548..4444)
                     /locus_tag="VQ514_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET2499701.1"
                     /translation="MKSLQSSLGNIETFKLDELCPKHEIQLTQIKGERHVVVGWNENG
                     EVIKEVRCIPPYCEQCQEEQKKQDEEDAIADNLNAKLYLQTYNVLMSNKSYVADEFKT
                     KTFDDFNAVTSEEKRFLEFAKGQVQKYLDGMRGNTLITGGTGIGKTLLSVAIAKGINE
                     GYKTKGEPKSVLFVSLTEMIKQIKEGWNYGKGATLTEFEATELMKSVDYLIIDDLGAK
                     NAVIKPKSDWEQDLLFDVLNNRENTIFNTNLDSDELKIVYNERNYSRILKGLEGNTFK
                     AFTIKDKRYSINQLKNKVKQLK"
     gene            complement(4456..5265)
                     /locus_tag="VQ514_000925"
     CDS             complement(4456..5265)
                     /locus_tag="VQ514_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HET2499702.1"
                     /translation="MAKTKVYFWLKIDKKFFDNIFIKRLKTVPGGYTMTVIYIRLMLE
                     SLESDCILYYEGYFNNLKEELALKLDVSEDDIDMTMAYFTKCGLIQIDEDKNAELPQA
                     KAMVMSETNWASYKREQRKKGKLEEVQPSLTFSNSCPTEIEKELEIEVDKEQSPTPST
                     INQDFVNLYKSFEAETGKALSPLQIQELQYMLEDFSPELIHEALKEAVSQGKANFAYI
                     KAILNRWKQDNLLTVELVRNSRAAREAKKQQTNQLEPTSYEDWIPTQEKPF"
     gene            complement(5340..5555)
                     /locus_tag="VQ514_000926"
     CDS             complement(5340..5555)
                     /locus_tag="VQ514_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499703.1"
                     /translation="MNELDISNTQAVFVTVILIGLLLYLNHRDRKKSAQLERENQQTI
                     ETPSEDLNPDYGRYIQLAGVDVWGRIE"
     gene            complement(5557..5757)
                     /locus_tag="VQ514_000927"
     CDS             complement(5557..5757)
                     /locus_tag="VQ514_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499704.1"
                     /translation="MTLDLDNMTQAEFDEAMAEIQLQSPNIFQLISDFVNKKVTSVEI
                     DDLLNMKRAEQVAYIKNYKARA"
     gene            complement(5754..5945)
                     /locus_tag="VQ514_000928"
                     /pseudo
     CDS             complement(5754..5945)
                     /locus_tag="VQ514_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841301.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5934..6224)
                     /locus_tag="VQ514_000929"
                     /pseudo
     CDS             complement(5934..6224)
                     /locus_tag="VQ514_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424675.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
     gene            complement(6394..6546)
                     /locus_tag="VQ514_000930"
     CDS             complement(6394..6546)
                     /locus_tag="VQ514_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499705.1"
                     /translation="MKIVTFKPTKQIDGGFYLPGIDILFVSDKADAKSTEDVILFISR
                     NGLNKS"
     gene            complement(6557..6823)
                     /locus_tag="VQ514_000931"
     CDS             complement(6557..6823)
                     /locus_tag="VQ514_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499706.1"
                     /translation="MEILSKEIQLQGLQLLKQTLETLVELEKQRSSKLDLISRKELMD
                     LLGISATTLDNWEDLALKRYQTPMDGAKKVFYRLSDVYLFLAIK"
     gene            complement(7034..7216)
                     /locus_tag="VQ514_000932"
     CDS             complement(7034..7216)
                     /locus_tag="VQ514_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET2499707.1"
                     /translation="MLITLSLAEKVRIKRARLQLTKKAVSEQLGIKSQTLTKVENGNY
                     DASKRIYESVMTWLVE"
     gene            7278..7781
                     /locus_tag="VQ514_000933"
     CDS             7278..7781
                     /locus_tag="VQ514_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2499708.1"
                     /translation="MYKMNRIKQLRKENNLTQRELANETKIPYRTIQRWENGETDIKS
                     DAAQVLADYFGVSIPHLLGYSDLIADTDSDIKAMAYSHLLTFVDEKQIKEFEKEFIRA
                     NDNSDTFGDLGSGEKALEKYYQALCYLVPPFRDILARYSFLSLDEKKSVYNIIVGLSN
                     NLSKENK"
     gene            7855..9018
                     /locus_tag="VQ514_000934"
     CDS             7855..9018
                     /locus_tag="VQ514_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HET2499709.1"
                     /translation="MNIIEKIKKNGQRVYYASVYLGVDQLTGKKARTTVTATTKKGVK
                     VKARDAINTFAANGYTVKDKPTITTYNELVKVWWDSYKNTVKPNTRQSMDGLVRVHLL
                     PVFGDYKLSKLTTPILQQQVNKWADKANKGEKGAFANYSLLHNMNKRILKYGVAIQVI
                     QYNPANDVIVPRKQQKEKAAVKYLDNKELKQFLDYLDTLNQSNYENLFDVVLYKTLLA
                     TGCRISEALALEWSDIDLESGVISINKTLNRYQEINSPKSSAGYRDIPIDKATLLLLK
                     QYKNRQQIQSWKLGRSETVVFSVFTEKYAYACNLRKRLNKHFDAAGVTNVSFHGFRHT
                     HTTMMLYAQASPKDVQYRLGHSNLMITENTYWHTNQENAKKAVSNYETAISNL"
     gene            complement(9172..10476)
                     /gene="eno"
                     /locus_tag="VQ514_000935"
     CDS             complement(9172..10476)
                     /gene="eno"
                     /locus_tag="VQ514_000935"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET2499710.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            10640..11086
                     /locus_tag="VQ514_000936"
     CDS             10640..11086
                     /locus_tag="VQ514_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HET2499711.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(11083..12198)
                     /locus_tag="VQ514_000937"
     CDS             complement(11083..12198)
                     /locus_tag="VQ514_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HET2499712.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIDVGGTASNDGGLGIAAGLGYQFYDRDGNVLP
                     ASGQSLLNLASVSTENCYKIPEGVQIHILADVVSPLCGHQGATYTFGNQKGRHPTMFA
                     VVDQAIQDFYEKFSPATLEIKGAGAGGGLAGGLCAFAQASIVSGIDTCLDLINFDKKV
                     SDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAEDLPPLPFENIQAV
                     FSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(12207..12536)
                     /locus_tag="VQ514_000938"
     CDS             complement(12207..12536)
                     /locus_tag="VQ514_000938"
                     /inference="COORDINATES: protein motif:HMM:NF019887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HET2499713.1"
                     /translation="MERLAKSLGIAYFTANQLEVKEGLLTGKLVGQIISPQVKKETLE
                     KWRKKLKLSKERTVAIGDGGNNLLMLKSAELGIAFCSKEMLKKEIPHHVDKRDFLEVL
                     PLIDCLE"
     gene            complement(12490..12852)
                     /locus_tag="VQ514_000939"
     CDS             complement(12490..12852)
                     /locus_tag="VQ514_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HET2499714.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHC"
     gene            complement(12911..14344)
                     /gene="glgA"
                     /locus_tag="VQ514_000940"
     CDS             complement(12911..14344)
                     /gene="glgA"
                     /locus_tag="VQ514_000940"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HET2499715.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGFERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(14341..15480)
                     /gene="glgD"
                     /locus_tag="VQ514_000941"
     CDS             complement(14341..15480)
                     /gene="glgD"
                     /locus_tag="VQ514_000941"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HET2499716.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVRTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVEPGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(15470..16612)
                     /locus_tag="VQ514_000942"
     CDS             complement(15470..16612)
                     /locus_tag="VQ514_000942"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HET2499717.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(16602..18530)
                     /gene="glgB"
                     /locus_tag="VQ514_000943"
     CDS             complement(16602..18530)
                     /gene="glgB"
                     /locus_tag="VQ514_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HET2499718.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEYGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAIVTELPDKKWRDGLWLARRKRWGFAERPVNIYEVHAGSWKRNPDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDIYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEVIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
BASE COUNT         5585 a         3937 c         3167 g         6127 t
ORIGIN      
        1 ttgtcttgtt cttgtttcaa ttgactatat aaattgtcta agcgaaacag ccgataagaa
       61 ttggcacaaa agcgaccgta tttttgttac caatacagga aaaacagttc atagttctat
      121 cttgagcaag tctctccagc gagcaaacga acgccttaaa aaaccaattc ccaaacatct
      181 gtcccctcac atcttcatac acatcactat tagcatctta tcagaaaata aaattccttt
      241 aaaaacaatc acggacaggg ttggtcatcc cgactctgaa gtcactaatt ccatctacac
      301 ccacgtcaca aagaacatga aagatgaagc aatcaatgta ctggataaag ttatgaaaaa
      361 gattttttaa aaagttttgt cccttttttg ccctctaaat acaaaaatag cccttcggat
      421 aaaatccgag gggctagaaa cgttgttaaa tcaacggccg aacttttgaa tttcatggtt
      481 cgggataaaa tagttcactg aactatttta ttttttaagg ttatcataat atcaaatagt
      541 tcaattaaat acgctaaatt actaatatac tttttacctt tttcattcta aaatgtaaag
      601 tacaaacaat tacaatatac tagaggggga gtaaaaaagg taataaaatt gacgagttca
      661 gcaggcaagg gaaatagcac ttttcatggt gttatttttt taattcagca atggagtagc
      721 cgcttaattg atagaaacaa aaagcaccta gtttcctaga tgctagcaca atagcccgga
      781 ttcgaaccgg aatctcctca attaaggtgc aatccctata tactactcat tgtgctttgg
      841 ttcattatat catacaatca actagctatc taccaactca tgatacgctc ccatattcgg
      901 taatggaata atactaaaat acaaatctga gtaagtttca aaccatttat tcagcttgtt
      961 ataggctggc gactgactaa caaataatat ccgttgacaa gctccgataa cattcatgtg
     1021 attatacaca tagacctctt taatggccgt taaaatatcg tcttcacttg tctgtaccct
     1081 aatatctgtt atctgcttaa tcgtagcgta ttttttataa gctagcatat ctttattttt
     1141 agcagcatca aacattttac actcaagaac actatactta ggtttttctt tatctctcat
     1201 gaaataccac ttgagccata aaattttttc tctgtgaatt actgaaatac gttcgatttt
     1261 tttctttggc atgattacct cctaaattat taatctaata gctttaacca ctcacttttt
     1321 gagattgttg tataaggttc atctagcgcg tgatataaag gttctaaacc tttgttaaat
     1381 cggatatagt ccattttatc ctcatgcttt ggaaaatata agattcctgt ttcatattca
     1441 tttagaccac ggttacacca atcatcaacg ataacctgca cttcccattc acgccacttt
     1501 ttaaggtttt ctaaaagttc aatatcacta aataactcac catcaacttg gaaaaattct
     1561 cccagatcat cgttaccctc gacgatgata acttcaggcg tatttctact acttaataat
     1621 tccttgagtt cttgtaactc tttgatctcc tttagatact tcctcaattt ctaacctcaa
     1681 ttcttcaatt tgtcttacta ctccaaaaat ttcatgagtt ttatatgatt gttcaagtat
     1741 agtttttgct gcttgagttc ttataaaagg gttaattttg gcatccatca taatatcatt
     1801 gagtacagaa acagcattgg atgaggctag atatagcatc tgtgtagttt tatccatcat
     1861 ctcatcttgt tttagtctca atgtcttttt aacagctgca acttttagat acttatgccc
     1921 tgttgccctt gacacccctg ctttttggca cgctttatct atcgttggct cagtaagcat
     1981 ggcatctatg aattttattt gtttagatgt aaggccatca ttttcatttc ctgccatcta
     2041 ttacctcctc gttatcaaaa taaaggggtt gctagcccct ctatttgttt aaatactcat
     2101 ccaaacgacg gtcaagctca ggggcatctg aatttactac catttcagtt gttacagctt
     2161 ggattttatc gccgttgata agcaaactgt aatgacaata tgaaagttgc cgcttaaaga
     2221 ataagattag actttctagc gcctgctctg cattgaataa ttccaagttg atagcaattt
     2281 ctggatattg ttcagcaatt gaccaaagtt ttttaatagc aagaggtttg ttcttgtatt
     2341 tttcaaaata acctagcaat tcatcctttg tcatcttcat actagctaac aaggtcagct
     2401 ctgccacgtc atcagctgtt acgctgagtt ctttttcttt taatgcctct agctctagat
     2461 catgctgttt ttcaatttct gcatgaattt tattataggt gtccctagaa taagtgtccc
     2521 cctctaactt ataagcctct agctggtctt tagcgtattt ttcgcctatc ataccatttt
     2581 gtaactgttt atgtaattcc tcttgtttag caatgtagcc agtcatgtac ccatgctttt
     2641 tcttgccaaa agccttaata tcatccttga ttgttgtaaa tgtcatgtta tcctccttac
     2701 gaaaaaataa aagaggtatg acaaaagagc gttaaactca attatcatac ctctggtttt
     2761 tccagtcagt atttattaac attgttctcc agtggtcact tccttataaa ctaactgccc
     2821 atcttgagct tttagcttaa tgctaccatg atctggaatt tcaatgtact ttattatacc
     2881 attttttgag tataaaacaa agcctttctc catcatttct aataatttat tatccttgtt
     2941 ttcagtcatg aatttctcct ttatttcaag tgtcccaacg ggtacggttg taagctgaga
     3001 cattttaggg ctatctttga gcgacaaaaa tgtcgaaaag tcttcagttc ctgtataacc
     3061 gttattagtt ttgtttgcta ctcatttttg agtcacaaat ctgttaagca aaaatgagta
     3121 atagctcgta agataagtaa atctaatact tgtattccta aagcgttgat ataactgata
     3181 ttttcaacta tccaacgctc tttactaccc tttttagtgt gctttagctc atttttgagc
     3241 ttttttgagc ttttttgagt tccattttgg gaatgcaaag caggcgcgca ccgtgcatgc
     3301 ttttttcttg ctcatagcct caccctcaga agtttgccga ccgagagcgt ggggcttttt
     3361 atttttgtat aattaaaaac agctgcacaa tttttgtaca gctacgaccg aggcaggggc
     3421 gacctgccgt actttacact agataatatc atctaggtag tccaactgta ttttatcata
     3481 ttaaaggcta cattacaatt tctcaccctg tgaagagtgt ctatttttgt ttaggtttgt
     3541 aagtcgatta cttcaactgc tttaccttat tttttaactg attaattgag tatcgcttat
     3601 ctttgattgt gaaagcctta aaagtgttac cctcaagacc ttttagaata cggctataat
     3661 tccgctcgtt gtatactatc ttgagttcat cgctatcaag atttgtatta aatatagtat
     3721 tttctcgatt attcagtaca tcaaaaagta aatcctgctc ccaatcgctc ttaggtttaa
     3781 taacagcatt ttttgcacct aaatcatcaa taattaagta atcaacagac ttcatgagtt
     3841 cagtagcttc aaactctgta agtgttgcac ctttaccata attccacccc tctttaattt
     3901 gtttgatcat ttcggttagg cttacaaaaa gcacactctt aggttctcct tttgtcttat
     3961 acccctcatt tatacctttg gcaatagcaa ctgataaaag tgtttttcca atccctgtac
     4021 ctcctgtgat aagcgtattt cccctcatgc catcaagata tttttgtacc tgaccttttg
     4081 caaattctaa aaatcgcttt tcttctgatg ttacagcatt aaaatcatca aaagttttag
     4141 ttttaaactc atctgctaca tagctcttat tgctcatcaa cacattataa gtttgcagat
     4201 atagtttagc attcaaatta tcagcaatcg catcttcttc atcttgcttt ttctgttctt
     4261 cttggcattg ttcacaatag ggtgggatac agcgaacttc ttttattacc tctccgttct
     4321 cattccaccc cactactaca tgtctttctc ctttgatttg tgttagctgt atttcatgct
     4381 taggacacaa ttcgtctagt ttaaatgtct caatatttcc taaactagat tgtaatgatt
     4441 tcattttctg acctcctaaa atggtttttc ttgtgttggt atccaatctt catagctggt
     4501 aggctctagt tgattggttt gctgtttttt agcctcacgc gctgccctgc tatttctaac
     4561 aagttccacc gtcaataaat tgtcctgttt ccaacggtta aggattgcct tgatgtatgc
     4621 aaagtttgct ttaccctgac tgacagcctc ttttaacgcc tcatggataa gctctgggct
     4681 aaaatcttct agcatatact gcaattcttg aatctgtaac ggtgacaatg ctttacctgt
     4741 ctcagcttca aaagatttat agagatttac aaaatcttga ttgatggtag agggagtggg
     4801 tgattgttct ttatctactt ctatctctaa ttctttctct atctctgttg gacatgagtt
     4861 ggaaaatgtc aaagatggtt ggacttcttc caattttcct ttttttcgtt gttcacgttt
     4921 ataactcgcc cagtttgttt cactcataac catagctttt gcctgtggta gttctgcgtt
     4981 tttatcttcg tcaatctgta ttaaaccgca ttttgtaaag tatgccatgg tcatatctat
     5041 atcatcttct gatacatcta attttagggc taactcttcc ttgagattgt taaagtagcc
     5101 ctcatagtat aaaatacagt cactttccaa gctctctaac atgagcctaa tataaataac
     5161 tgtcatagtg tacccaccag gtaccgtctt tagtctcttg ataaaaatat tatcaaaaaa
     5221 tttcttatca atttttagcc aaaaatagac ttttgttttt gccatcgtcc tctcccttgc
     5281 tcatttataa ttctctacct tgattagtct taatactcgc ttgtcattca ttgttagact
     5341 cattcaattc ttccccatac gtctacccct gcaagctgga tatatcgccc ataatcaggg
     5401 tttaaatcct cgctaggtgt ttctatcgtc tgttggtttt ctcgctccag ttgggcgctt
     5461 tttttgcggt ctcggtggtt taaataaagc agtaagccaa tcaaaatcac ggtaacaaaa
     5521 accgcctgtg tattgcttat atctagttca ttcatgttat gccctcgctt tgtaattctt
     5581 gatgtatgcc acttgttcag ctcgcttcat attcaacaag tcgtctattt ccacactggt
     5641 tactttttta tttacaaaat cagaaatcag ttgaaaaatg tttggacttt gtagctggat
     5701 ttcagccatt gcctcatcaa attctgcttg tgtcatgttg tctaaatcta gtgtcattgc
     5761 attgcctcct caaacttctc tataagacaa cttttatttg ctttctgagt tccattttta
     5821 gagttaaaaa gaatatattt taaggttaca gtagcctcta aatactcctt ttcagcatgc
     5881 tctatatacg cctgttgctc tgcttcgttc tcaaaaaagt gcttagctta gcgtttaaag
     5941 aatgctttga ctaggtttag tttgaattgc cgtactgctt cggtattctt tagataagtg
     6001 atcagcaaag tagcctgttg ctcgttcaga atataggatt ttttaggttg tcctctagtg
     6061 tctaatttat ggattttaaa tccaagtatt cccaactctt caaagtcaac cttattttct
     6121 cttattaagc gtgtgatagt gtggtgttgt acgtcagcac attcagcgat gatctcgctt
     6181 gtggtatacg gctctttctt gccgtccatg tagactagtt ccattatggt tctacctcct
     6241 gtataaattt agctagctta ctttttaatt gtctcctcta gcccttttta gcctctacaa
     6301 cggttttggc taacgtgtta gaggctttta taacggcctg tatagctgtc tctatttcct
     6361 ccgtggaata gtagctttgt tttctaatat cattcaagac ttgtttaacc catttcttga
     6421 aataaataaa attacatctt ctgtactctt ggcatctgct ttatctgaga caaatagaat
     6481 gtcaatgcct ggtagataaa acccaccgtc tatttgttta gttggtttga aagtaactat
     6541 tttcatttca taactcctat tttattgcta aaaataaata cacatctgac agacgataga
     6601 atactttctt agctccatcc atcggggtct gatatcgttt aagagcaaga tcctcccagt
     6661 tatcaagggt tgtagcactt atacctagca gatccattaa ttctttacga gaaattaaat
     6721 ctaacttact agatcgttgt ttttctagct caactaaagt ttcaagagtc tgtttaagaa
     6781 gctgtaaacc ctgtaactgt atttctttag acagaatttc catttatatc ctccattttc
     6841 ctttttataa attaagttgt ctttttcctc taattttgat aaaataagaa gagattattt
     6901 agcaaagggg gttgtccctt tgctaaatac ctaacaaaac cacgcgcctt gcctttgctt
     6961 gagttttgtt atttttcctt agccgtgtac gttggtttgg tcgcctgtac atggcttttt
     7021 acttgcgtta aatttattct actagccaag tcatcacgct ctcatagata cgcttagagg
     7081 catcatagtt tccattctca acttttgtta gtgtttgaga tttaataccc aactgctccg
     7141 atactgcttt tttggttagc tgtaatcttg cacgcttgat acgtactttt tcagcaagtg
     7201 atagtgtaat caacattggt ttcacctccc ttttttctcc atttttggca ttttgtgtta
     7261 taatctcttt agaaaggatg tataagatga atagaattaa gcaattacga aaagaaaata
     7321 atcttactca aagagaactt gctaatgaga ctaaaatccc ttatagaacc atacaacgtt
     7381 gggagaatgg ggaaactgat ataaaatccg atgctgctca ggtacttgct gactattttg
     7441 gagtatcaat tcctcactta ctaggttata gcgatttgat agcagataca gatagtgata
     7501 ttaaagcaat ggcatactca catctactaa cttttgtaga tgaaaaacaa atcaaagaat
     7561 ttgaaaaaga gttcattcgt gcaaacgata atagtgatac ttttggtgat ttaggaagtg
     7621 gtgaaaaggc gttagaaaaa tactatcaag cattatgcta tttagttcct ccttttaggg
     7681 atatattggc taggtatagc tttttgtcac tagatgaaaa aaaatcagtc tataatatta
     7741 ttgttggttt aagtaacaac ttatcaaaag aaaataaata actttaaaca tcatgtataa
     7801 atacctaaca aaaccacgcg ccttgcctgc tgatggaaag aaaggtacaa atacatgaat
     7861 atcatagaga aaattaaaaa gaatggccaa agagtttatt atgctagtgt ttatctaggc
     7921 gttgaccaac taacgggcaa aaaagcccgt acaactgtta cagcaaccac taaaaagggc
     7981 gttaaagtaa aagcgcgtga tgcgatcaat acttttgctg ctaatggcta tacagttaaa
     8041 gataagccga caattacaac atacaatgag cttgtaaaag tttggtggga tagttacaag
     8101 aatacagtta agccaaatac tcgccaatcc atggatggat tggttagagt gcatttattg
     8161 cctgtatttg gcgattacaa gctatctaaa cttactacgc ctattcttca acagcaagta
     8221 aacaaatggg ctgacaaggc aaataaaggc gaaaaagggg catttgctaa ctactccttg
     8281 ctccataaca tgaataagcg tattttgaaa tatggcgtag ctatccaggt aatacaatac
     8341 aacccagcta atgatgtaat cgttccacgc aaacagcaga aagaaaaggc tgctgtcaaa
     8401 tacttagaca acaaagaatt aaaacagttt cttgattatt tagacactct gaatcaatca
     8461 aattatgaga acttatttga tgttgtcctg tataagactt tattggccac tggttgccgt
     8521 attagtgagg ctctagctct tgaatggtct gatattgacc tagaaagcgg tgttatcagc
     8581 atcaataaga cactaaaccg ctatcaggaa ataaattcac ctaaatcaag cgctggttat
     8641 cgtgatatac caatagacaa agccacatta cttttactga aacaatacaa aaaccgtcaa
     8701 caaattcagt cttggaaatt aggccgatct gaaacagttg tattctctgt atttacggag
     8761 aaatatgctt atgcttgtaa cttacgcaaa cgcctaaata agcattttga tgctgctgga
     8821 gtaactaacg tatcatttca tggtttccgc catacacata ctactatgat gctctatgct
     8881 caggctagcc cgaaagatgt tcagtataga ttaggccact ctaatttaat gatcactgaa
     8941 aatacttact ggcatactaa ccaagagaat gcaaaaaaag ccgtctcaaa ttatgaaaca
     9001 gctatcagca atttataaaa aataagggtg acccatttcc gggctaccct cttactatac
     9061 caaaaattag taggggtagt aaaaagggta ttaaattata aaaagcacta agggaaagcg
     9121 ccccaaagtg cttatttcaa aggctttata gcctataatc acataaggag attatttttt
     9181 aaggttgtag aatgatttca atccacgata ttcagctact tcgccaagtt ggtcttcgat
     9241 acgaagcaat tggttgtatt tagcgatgcg gtctgtacgt gaaagtgaac cagtcttgat
     9301 ttgtcctgcg ttagttgcaa ctgcaatatc agcgattgtt gaatcttcag tttcacctga
     9361 acggtgtgat acaacagcag tgtaaccagc ttctttagcc atttcgatag cttcaaaagt
     9421 ttcagtaaga gtaccgattt ggttaacttt gataaggatt gagttagcag caccttcttg
     9481 gataccacgt gcaaggtagt cagtgtttgt tacgaagaag tcgtcaccaa caagttgtac
     9541 tttcttacca agacgttcag taagagcttt ccaaccatcc cagtcgtttt catccatacc
     9601 atcttcaata gtgatgattg ggtatttgtt aaccaattct tcaaggtagt cgatttgttc
     9661 tgcagatgta cgaacagcag caccttcacc ttcaaattta gtgtagtcgt aaactttacg
     9721 ttctttatcg tagaattctg atgaagcaca gtcaaatccg ataaatacgt ctttacctgg
     9781 tacatatcca gcagcttcaa tcgcagcaag gatagtttca acaccatctt cagttccttc
     9841 gaaacgagga gcgaatccac cttcgtcacc tacggcagtt tccaaaccac gtgatttaag
     9901 gattttctta agagcgtgga agatttcagc accgtaacga agggcttctt taaatgttgg
     9961 cgcaccaact ggcaagatca tgaactcttg gaaagcgatt ggagcgtcag agtgagaacc
    10021 accgttgatg atgttcatca ttggagttgg aagaacttta gtgttgaatc caccaaggta
    10081 gctgtaaagt gggatttcaa ggtagtcagc agcagcacga gctacagcga tagacacacc
    10141 gaggattgca ttcgcaccca atttaccttt gttaggagta ccgtcaagtg cgatcatagc
    10201 acggtcaata gcttgttgat cacgtacatc gtagccaatg atagcttcag caatgatgtt
    10261 gtttacgttg tcaacagctt tttgtgtacc aagaccaccg taacgagatt tgtcaccgtc
    10321 gcgaagttca actgcttcgt gttcaccagt agaagctcct gatggaacca taccacgtcc
    10381 gaaagcacct gattcagtgt aaacttctac ttcaagtgtt gggttaccgc gtgagtctag
    10441 gacttcgcga gcgtaaacat cagtaataat tgacattttt tactctcctt atgagttaaa
    10501 ttttttacac ctctataata ccttaaaacc cctccttttt caagaaaaaa cgttatcttt
    10561 gtgcaacttt tccttaactt tataaagtaa tcgctttctt ttgtctgttt tattctaact
    10621 tttatgatat actgttttca tgacagattt atcaaaacaa ttacttgaaa aagctcatgg
    10681 tgggttaaaa ataaatccgg atgagcaaag acgctatctt ggtacttttg aggaaagagt
    10741 tcttggatat gtagatattg acacagcaaa tagccctcag ttagaaaaag gctttttatt
    10801 tattttagaa aaccttcagg aaaaagcaga gccactattt gtgaagattt caccaactat
    10861 cgaatttgat aagcaagttt tctacttaaa agaagcaaaa gaaactgata gtcaagccac
    10921 catagtatct gaagagcata ttacttctcc ttttggcctg gttattcata gcaatgcacc
    10981 agttcaagta gaagaaaaag accttcgact tgcttttcca aaactttggg aagttaaaaa
    11041 ggaagaacca gccaaaacat ccttatggaa gaaatggttt agctaaatct tgcacatatt
    11101 taataagtgc ccaatattgg cagccgtgcg ctccagatag agactggcat ttttcaaact
    11161 atcttctaaa ggttcacttt tctccaaaat agaaaagaca gcttggatat tttcaaatgg
    11221 taggggaggt aaatcttcag caagactacc gcaaatagca ataacaggaa ctccaacagg
    11281 ggttcttttt gcaacaccta taggcgcttt cccagcaaag ctttgactat caagtcttcc
    11341 ttctccaaca acaaccaagt cagcatctga aactttctta tcaaagttga ttaagtccaa
    11401 gcaggtatca attccagaca cgatacttgc ctgagcaaaa gcacacaaac caccagcaag
    11461 gcctccacct gctcctgctc ctttaatttc taatgttgca ggtgagaatt tttcataaaa
    11521 atcttggatc gcctgatcta cgactgcaaa catagtcgga tgtcgacctt tttgattgcc
    11581 aaaagtataa gtcgcacctt gatgaccaca taagggactt acgacatctg ctaaaatatg
    11641 aatttgaaca ccttcaggaa ttttatagca attttctgtt gaaacagaag ctaagtttaa
    11701 taaggattga ccggaagcag gcaagacatt tccatcccta tcataaaatt gataacctaa
    11761 accagcagca atccccagtc ctccatcatt actggccgtg ccaccaacat cgatataaat
    11821 atctttaatc cctttagaaa tgagatggag aatcaactct ccaataccac aagtttggat
    11881 ttgaagtgga tttcgtttct ctagcggaat ttttccaaga ccaaccaagt cagctacttc
    11941 aaatagtgcc agttcccctt tttgaaaata gcgcatggct tctttttgtc caaaagggtc
    12001 tgtcacttgg atccattttt cttttaggtc aagagaatgt cggatagcat ctacagtacc
    12061 ttctccccca tcaccaacag ggcagaggag acattctaca tctgctatcg attgttggaa
    12121 gcctcttttt attgcttcag ctacctgttg agctgtcaag ctttccttaa acgaatccgg
    12181 tgcaattaca atcttcatat tttccctcat tctaaacagt caatcaaagg aagaacttct
    12241 aaaaaatccc tcttgtcaac atgatgtggt atttcttttt tgagcatttc tttggaacaa
    12301 aaggcgattc ctaactccgc cgacttcaac attaatagat tattgccccc atcaccgatt
    12361 gccaccgttc tttctttaga aagttttagt ttctttctcc atttttccag agtttctttt
    12421 ttgacctggg gacttataat ttgtccaact aattttcctg ttaaaagacc ttctttgact
    12481 tcaagctggt tagcagtgaa ataggcaata ccaagggatt ttgctaatct ctccaactat
    12541 tggtgtaaat ccaccagaca ccagaccaac taggatacca ttcttttgga gaatagagat
    12601 gaactctggg acatttagcg atagatgaat tgagttgaag acgttatcaa agaccaaaat
    12661 aggaagacct tccaacaagg acactctttt tcttaaactg ctttcaaaga ccaactctcc
    12721 tcgcattgct cgacttgtaa tctgcgaaat ttctgcctca tgacctgcct ctctccctaa
    12781 aagatcaatc acttcttcta ggattaaggt tccatctaca tccaaaacac acaagccttt
    12841 tacttgagac atcagttctc ctctctaaac agcctaaaaa tcgtatgaag tcatcatacg
    12901 attttatcta ttaattaact aaactatggt acaagtcaag gtatgacttg caggctgtat
    12961 cccatgagaa gtcactctcc atagcttgtt tttgtaggtt tctccaaatg tctggatggt
    13021 ttctatacaa gtccaatgct gtttggaaag tccaatttaa ccaataagga gatagattgt
    13081 caaagctaaa gccagtaccg cttccttcga ttggattgaa agcgcgaact gtatctcgca
    13141 agcctccaac ttcatggacc aatggcaagg ttccataacg catagccatc atttgagaca
    13201 agccacacgg ttcaaaacga cttggcatga ggaagaggtc acaagcagcg tagatttcct
    13261 gagcaagttt gacatcaaaa gtgatatttg ttgatagctt gtctgggtaa atctgagcaa
    13321 accatgagaa agctccttca aaggctggat cgccagttcc caaaagaaca atctgaacat
    13381 cttcttgcaa gatatggtga agactttcga ccaccacatc aaaacctttt tgacgtgtca
    13441 aacgagaaac aattcccacc agtggaacgt ctgctctaac aggcaagcca actctttctt
    13501 gcaattttgc cttatttttg gctttcccag acaaatcttc ctgattgaaa tgatagtcta
    13561 aaagagcatc cgtctgagga ttataaagat cagcatcaat cccattcacg ataccagata
    13621 ctttaccaga ctccatttta agaatctgat ccaaattaca tccaaactga ctagtcataa
    13681 tttcatgagc atagctaggt gaaacggttg aaacacggtt cgcatagaga atacctgcct
    13741 tcatccagtt cagacagttg ttccatcgaa gggtgccatc agcgtaacgt tcaaagccaa
    13801 ctccaaacaa atcacccaac attccttctg aaaattgtcc ttggaattct aaattatgaa
    13861 tggttaaaac tgtttcaatg tcctcatagg cttgaatcca acggtatttt tccttcaaca
    13921 agaaaggaat catagctgta tggtagtcat gaacatggag aagatcagga ataaagtcaa
    13981 tcctttccat agcctcaatg gcagccagtt ggaaaaaggc aaagcgttct ccgtcatcaa
    14041 aatcaccgta aacatgacca cggaagaaat aatattgatt gtcaataaag tagaaggtta
    14101 caccatttaa tactgttttc ttaattccac aatactgtct gcgccaacca acgctcacct
    14161 caaaatgaag cacatcttca atctgatttc caaatttagc ctctaccata tcatagtagg
    14221 gtaaaatcac tgcaacttcg tgcccagctt ttaccagtga ttttggaaga gcgccaatga
    14281 cgtctcccaa accacctgtt tttgaaaagg gtgcaccctc tgctgctaca aataaaattt
    14341 tcatgaatga atatcctctg ttactttagc acctttctta accacaactg gatgttctgc
    14401 agttcctcga atcacaacac caggctcaac ttcaacccct ttgtccaaga tagcatattc
    14461 gacctgagcc ccttctccaa taacaacacg agggaagagc aggctatctt taaccaagct
    14521 atccttatgg acatggatat tacgtgatag aacagaatta gctacttgac cttcaataat
    14581 actaccagag gcaaactgag aagttcttac cttagatgta ttagcatagt aagttggctc
    14641 ttcgtttttg acctttgtat aaatcttttg gtttggtgag aaaagagaat agaatttttg
    14701 tgattcaagc atatcgatat tcgcttgata ataagattta acagagtgaa tattggctag
    14761 atagcccgtg tactcgtagg cgaaagctcc ctcttttaca gccaaatccc gtaaaacata
    14821 gcgcaatttc tctggatgtt cttttttagc ttcttcttcc aagtgttcaa tcaaccaagg
    14881 tgtatcaacg acaaagatat ctgtagacat attgaacgtt tcagctgttg acttgctatc
    14941 aaagagttta tgagaaagaa catggtctgt ttcatctaca tccaagattg catttacttc
    15001 tgaaatatct ttcttagcta gttttttata aactacagtg ataggctctt ttgttgtact
    15061 atgtaggtgg aaaacttggt tcaaatcaat gttaataaga acatcgcagt tgagggcaac
    15121 cgtttggttt gagccagaac gtttcaaata agtaagaagc tgttggtagt attcttttcc
    15181 aactgtacta ctttctacac gggtattgta aattcctaga tagtaatggc taagaagggt
    15241 tgataagccc cactcgcgtc ctgaacgaat atggtcaaat actgagctga tattatcctg
    15301 ctggaaaata ccaaagacac tacgaacacc tgcattagca aggcttgaaa gtgggaagtc
    15361 aatcaaacga tatttcccac caaatggcaa acttgctact ggacggtggt ccgtcaatgt
    15421 cgacatattg tgaaaaccaa ctgtatttcc taaaatggca gaatatttat caatcttcat
    15481 ctgttgctac ccccactact tcattatatc ctacaacttg tacttcatct gttccatcaa
    15541 tttcgacacc gtcagaaata atcgcacctt caccaataat ggcacgttta atcttagctc
    15601 cttgaccaat gatagctcca ctcatgataa ctgaatcaag gacttccgct ccttcgcgaa
    15661 cttgcgcgcc tgttgaaagg atagaatgtt taacagttcc atcaacgaaa catccgtcta
    15721 caactaatga gtcttccaca tgagcatttg ccccgaggaa gtttggtggt gaaatcaagt
    15781 ttcttgagta aatcttccat tgacggttac gactatccaa ggcattttct ggagaaatat
    15841 actccatgtt cgcttcccaa agtgactcaa tagtaccaac atctttccaa taaccactaa
    15901 attcgtaagc ataaacactt tcacctgact caaggtaatt tggaatgaca tttttaccaa
    15961 agtctgacat gccaaccttg ctcttttcag cagcgactaa catattacga aggcgttgcc
    16021 aatcaaaaat gtagattccc atagaagctt ttgtagattt aggttgagct ggtttttctt
    16081 caaattcaac aatacgattg ttagcatctg tgttcatgat accaaaacgg cttgcttctt
    16141 taagagggac gtctaaaact gctactgtca agctggcatt attatcctta tgagactgga
    16201 gcatatcatc atagtccatt ttgtagatgt gatccccaga caaaatcaag acatactcag
    16261 gattgacact gtcgatatag tcgatatttt ggtaaatagc gtgactagtc ccctcaaacc
    16321 aacgatttcc ttcacttgca gaataaggtt gaagaataga gacacctgaa ttaataccgt
    16381 ctagtcccca gcttgaacca ttcccaatat ggttgttgag agcaagtggt tgatactgtg
    16441 taacgacccc aacattatga atccctgagt tggcacagtt tgatagggca aagtcaatga
    16501 tacggtagcg cccaccaaat tgcacagctg gttttgcgat gctttgagtg agtttaccga
    16561 gacgagttcc ttgcccacca gcaagaatca aagctaacat ttcatttttc attttctact
    16621 cctttttggt ttttatttgt gacggtttta gtagatttca agcgacgttt gattttccat
    16681 acacttgctc ccatagccgg tagggtaaag gttaaggtct gctcataatc tttccatagt
    16741 ccttcttgcg tttgaacagt ttgattatgt tctttccaaa cgcctcccca ctcttccaac
    16801 tcagtattcc atacttcttc gtaaattcct gcaacgggta gtccgattgt aaaatctttc
    16861 cgctcaacag gtaccatgtt aaagatacag actaacattt ctcccttttt acccttacga
    16921 ataaaggaaa gaacactctg gtctcgatta tccgcatcaa tgacttcaat accatcatag
    16981 ctggtatcga tttcccacag acagcgatga tctttgtaaa actggtttag ctgagaagtg
    17041 aaatacttca tcttagcatt cattgggtct tctaggttag accattccaa ctgttcttca
    17101 gatttccatt ctaggaattg accgtattcg ctacccatga agagcaattt cttaccaggg
    17161 tgacaaattt ggtacgtata gagattgcgc aagcctgcga attgattgta acgatctccc
    17221 cacatcttat gcatcatact cttcttgcca tgaaccactt catcgtgcga gaatggcaag
    17281 agataattct ccttgaaaac atacataaag ctgaaagtca ccaggttaaa gtcatattta
    17341 cgataaatcg gatcttcttc gtagaaacgg aggatatcat tcatccagcc catgttccat
    17401 ttgtagtcaa atcctagacc accaatctct ttcattcccg taatcttggt tgcagacgaa
    17461 ctttcttctg caatcatcat cacatctgga tattctaact taataacttc attcaagcgt
    17521 tgaaggaaat aataaccttc atagttgaga tttccgccat ctttattagg tgtccatgga
    17581 gcatcatcat agtccaaata gagcatgttg ctaacagcat ccacacgaat accatccaaa
    17641 tgatagatat caatccaatg cttaatgcaa gaaattaaga aggactggac ttcatttttt
    17701 ccaaggtcaa aattaagggc accccaacca tggttatgag ccttattatg gtcttggtat
    17761 tcaaaagtcg gtgtcccatc ataataggct aaggcatcat cattgatggt aaagtgacct
    17821 ggtacccagt ccacaataac cccaatatta tgggtatgac actcctcgac aaaatcttga
    17881 aactcctctg gtctaccata ggcatgctct aaagcgaagt aacccataag ctggtacccc
    17941 caactcaaac ctagtgggtg ggacatcaag ggcataaact caatatgagt atagttcatc
    18001 tcaactaggt aaggaatgag ttcatctttc aattgggcaa aactataagg actgccatca
    18061 ggatttctct tccaagagcc agcatgaacc tcgtaaatat tgacaggacg ttcagcaaag
    18121 ccccaacgtt ttcttcgtgc cagccaaagt ccatccctcc atttcttatc aggaagctct
    18181 gttacgattg cccctgttcc tggacgagcc tcatacctga cagcaaaagg gtcaatcttc
    18241 atcagttgat gaccattttg acgtgtgaca tgatatttgt aaatatgccc ttcttgagcc
    18301 atattggtaa agacttccca gaccccaaag tcatttctta ccattggaat ctgattttca
    18361 atccaattgg taaaatcacc aaccaagtga acagcctgag cattaggtgc ccaaacacgg
    18421 aaggtatagc catactctcc atttagttct tccctatgtg ctcctagata atgttggaga
    18481 taaaaatttt cacccgtcat aaaggttttt aatgcttctc tattatccat atactccctt
    18541 tctcctgtaa gcgttttcta tgtttttatt atactacctt tttagagaag attcaagtaa
    18601 attactatac ttctttaatt attttgaaaa tctacaacaa gttcacttac tcgttcaatt
    18661 gtaaatcaat attttttcaa aaaattgcga aaacaccttt ctttttctac tatagtgaaa
    18721 tgaaataaaa catgcgcaaa tcgattaagg aatttaatct aatttctaac aatgtcttag
    18781 aaatcaaagt gtactatttt aacttcaatg tactat
//
LOCUS       DAVBCW010000032        18608 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVBCW010000032 DAVBCW010000000
VERSION     DAVBCW010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18608
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.032"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            <1..505
                     /locus_tag="VQ514_000944"
     CDS             <1..505
                     /locus_tag="VQ514_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2499719.1"
                     /translation="DELTILQILYGASLISFLWMYGQRKQVIKVNMKTRIQWLGIGFV
                     SLLIISLCFSLIHAQESTNQANLIGLQHQIPWFSFLLFLINASMVEEFLYREILWNLV
                     RKLDIRVALTSVLFALAHHPGTIIAWCLYVSLGMFLGLVRYKLDLWGSMGLHLVWNLL
                     VYSLLLF"
     gene            905..1714
                     /locus_tag="VQ514_000945"
     CDS             905..1714
                     /locus_tag="VQ514_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HET2499720.1"
                     /translation="MLLQKELIPMIEANLPNMAYAEKDIAKFFLKQQPLNNYSCKALC
                     ECLNVSKATLTRFAKKCGFKGFRQFIFKYQEMIREKEKLALYTEATEKVLSDYEEMLR
                     KTYTVLDEVQLERIAEMIETAERVYLYGKVSSVLALQEMKMRFMRLGVIGEVLSDEDM
                     ILWNSLLLNENCLVIGASVSGQTDIVLEGLQKAADKGAKTVLMTTRKFDEEDCFFDEL
                     LLLASTNHLSYGNRISPQFPILLITDCLFSHYLESPERQYYYNQTIIHKEE"
     gene            1719..2420
                     /locus_tag="VQ514_000946"
     CDS             1719..2420
                     /locus_tag="VQ514_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HET2499721.1"
                     /translation="MSQIWTKEKFISQVHGGVIVSCQALPGEPLYNEEFSLMPFMAKA
                     ALEAGAVGIRANSVRDIKAIQKVVDLPIIGIIKRDYPPQEPYITATMKEVDELVECGT
                     TVIAFDATLRPRYDGLVINEFIKKIKEKYPNQLLMADVSNFDEGLYAFKSGVDFVGTT
                     LSGYTSTSVQSDEPDFELMKKLADFNIPVIAEGKIHYPEQLKKAYSLGVTSVVIGGAI
                     TRPKEIAQRFINVIK"
     gene            2445..3362
                     /locus_tag="VQ514_000947"
     CDS             2445..3362
                     /locus_tag="VQ514_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HET2499722.1"
                     /translation="MKDLTKYKGVIPAFYACYDENGEISQERVKSLVQYFIDKGVKGI
                     YVNGSSGECIYQSVEDRKQIIEAVMEVAKGKLTVINHVACNNTKDSIELAKHSESVGV
                     DAIAAIPPIYFKLPEYSIAAYWNAMSEAAPNTDFIIYNIPQLAGVALTGSLYATMRQN
                     PRVIGVKNSSMPVQDIQMFVAAGGEDYIVFNGPDEQYLGGRLMGAEAGIGGTYGVMPD
                     LFLKLESLIQERDLDTAKKLQYAINEVIYKMISGKANMYAVAKEVLRLNEKLDLGSVR
                     QPLEALAEGDLEVAKQAAELIQQARKEFL"
     gene            3390..4931
                     /locus_tag="VQ514_000948"
     CDS             3390..4931
                     /locus_tag="VQ514_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HET2499723.1"
                     /translation="MGTTGFTIIDLIILIVYLLAVLVAGIYFSKKEMKGKEFFKGDGS
                     VPWYVTSVSIFATMLSPISFLGLAGNSYAGSWILWFAQLGMVVAIPLTIRFILPIFAR
                     IDIDTAYDYLDKRFNSKALRIISALLFIIYQLGRMSIIMYLPSAGLSVLTGIDINILI
                     ILMGAVAIVYSYTGGLKSVLWTDFIQGVILISGVVLALFVLMANIKGGFGAVAETLAN
                     GKFLAANEKLFDPNLLSNSIFLIVMGSGFTILSSYASSQDLVQRFTTTQNIKKLNKML
                     FTNGVLSLATATVFYLIGTGLYVFYQVQNAGSAASNIPQDQIFMYFIAYQLPVGITGL
                     ILAAIYAASQSTISTGLNSVATSWTLDIQDVISKNMSDDRRTKIAQFVSLAVGLFSIG
                     VSIVMAHSDIKSAYEWFNSFMGLVLGLLGGVFILGFVSKKANKQGAYAALIVSTIVMV
                     FIKYFLPPTAVSYWAYSLISISVSVVSGYIVSVLTGNKVSAPKYTTIHDIPEIKADSS
                     WEVRH"
     gene            4950..5408
                     /locus_tag="VQ514_000949"
     CDS             4950..5408
                     /locus_tag="VQ514_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET2499724.1"
                     /translation="MIISEQSDFRRYASINKHFSKVCDFLENTNLTDLVDGKVDIDGE
                     NVFANCMIYLADGVPGDIFETHKKYLDIHIVVENTEKMAVTSPVRAQSRVPFSEEKDI
                     AFYDSKDYQIVELLPGNMLVTFEEDLHQPKIHCNDEPVKKLVIKVLNEEK"
     gene            5410..7632
                     /locus_tag="VQ514_000950"
     CDS             5410..7632
                     /locus_tag="VQ514_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HET2499725.1"
                     /translation="MKKNIKQYVTLGTAVVLSAFVANSVAAQETETSEVSTPELVQPV
                     APTTPISEVQPKSGNSSEVTVQPRTVETTVKDPSSATEETPVLEKNNVTLTGGGENVT
                     NELKDKFTSGDFTVVIKYNQSSEKGLQALFGISNSKPGQQNSYVDVFLRDNGELGMEA
                     RDTSSNKNHLVSRPASVWGKYKQEAVTNTVAVVADSVKKTYSLYANGTKVVEKKVDNF
                     LNIKDIKGIDYYMLGGVKRAGKTAFGFNGTLENIKFFNSALDEETVKKMTTNAVTGHL
                     IYTANDTTGSNYFRIPVLYTFSNGRVFSSIDARYGGTHDFLNKINIATSYSDDNGKTW
                     TKPKLTLAFDDFAPVPLEWPRDVGGRDLQISGGATYIDSVIVEKNNKQVLMFADVMPA
                     GVSFREATRKDSGYKQIDGNYYLKLKKQGDTDYNYTIRENGTVYDDRTNRPTEFSVDK
                     NFGIKQNGNYLTVEQYSVSFKNNKKTEYRNGTHVHMNIFYKDALFKVVPTNYIAYISS
                     NDHGESWSAPTLLPPIMGLNRNAPYLGPGRGIIESSTGRILIPSYTGKESAFIYSDDN
                     GASWKVKVVPLPSSWSAEAQFVELSPGVIQAYMRTNNGKIAYLTSTDAGTTWSAPEYL
                     KFVSNPSYGTQLSIINYSQLIDGKKAVILSTPNSTNGRKHGQIWIGLINDDNTIDWRY
                     HHDVDYSNYGYSYSTLTELPNHEIGLMFEKFDSWSRNELHMKNVVPYITFKIEDLKKN
                     "
     gene            7672..8781
                     /locus_tag="VQ514_000951"
     CDS             7672..8781
                     /locus_tag="VQ514_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET2499726.1"
                     /translation="MVNYGIVGAGYFGADLARSMNKIEDAKVVAVFDPNHGEEVAQEL
                     GSDVCASLDELVAREDIDCVIVASPSYLHREPVVKAAQHGKHVFCEKPIALSYEDCKA
                     MVDACKENNVIFMAGHIMNFFNGVHHAKELITQGKIGKVLYCHAARTGWEEQQPTVSW
                     KKLRSQSGGHLYHHIHELDCIQFIMGGLPEKATMVGGNVYHKGENFGDEDDMLIVNLE
                     YSDDRYAVLEYGNAFRWGEHYVLIQGTEGAIKLDLFNTGGTLRVKGEGESHFLVHETQ
                     EEDDDRTAIYTGRGMDGAIAYGKPGVRCPLWLQTCIDKEMEYLHDIIKGGEITEEYEK
                     LLNGVAALESIATADACTLSVKEDRKVSLSEITNA"
     gene            8938..9828
                     /locus_tag="VQ514_000952"
     CDS             8938..9828
                     /locus_tag="VQ514_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2499727.1"
                     /translation="MKIIGIDIGGTTIKADLYNEFGTSLNQFKEIETIIDYDLGTNQI
                     LNQVCDLIGEYILNHSIDGVGISTAGVVNANTGEIIYTGYTIPGYIGVNFTFEIEKRF
                     GLSTFVENDVNCAALGELWKGQAKDKKNVVMVTIGTGIGGSIIVNGQIVNGFNYTAGE
                     VGYIPVGNSDWQSKASTTALIHLYQKKSLKTNQTGRTFFTDLRAGDKVAKETFEIFVE
                     NLTKGLLTITYLLNPEILILGGGILAKKDILLPEIQSSLAKNAMDNRFLPKNLVAATL
                     GNEAGRIGAVKNFLDRISNK"
     gene            9853..10176
                     /locus_tag="VQ514_000953"
     CDS             9853..10176
                     /locus_tag="VQ514_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499728.1"
                     /translation="MTNSVFSTMQDIENVATDIIKSYDNEIYTYKAVSQEELEKLEKS
                     YDEKSHEELVSIESNLEMKQQNLIDEVNKTIKENYEKIQYISSSRRGEFVEKIIGRVV
                     EKYGY"
     gene            10166..12157
                     /locus_tag="VQ514_000954"
     CDS             10166..12157
                     /locus_tag="VQ514_000954"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HET2499729.1"
                     /translation="MAISQMKRISLLFSKSSLDDVLKTIQELESVQFRDLKVQDNWSE
                     ALEKDEVVFPTIQISHTSNSNHGVIEGNDALIYLMNQQQYLEATVEKLQEYLPKENTF
                     KLLQQPPITTSYEELEKFGKANVAEGVLKKVNRQINRVHELERHIQSNNEEIERLIKW
                     EKLEIIPAHLEQFFFCKGKVGTIPRTVDNRLYNSFLENNIEVQEIFSNDREYGVVVFY
                     QSSCSIDFDEYLFEPFDYSRKELPKQRVVDLDQENMQLITEKENIIASLQDSKNNLID
                     LQWQIDYILSIYARQISKNNLLSTPHLVALEGWIEETRILYFIKVMDERFGHSIYIYE
                     SETLSDNQDEIPIKLTNHSLIEPFELLTEMYALPKYYEKDPTPVLAPFYFTFFGMMVA
                     DLGYGLLLFLGTMLALKIFHLPSETKRFLKFFNILGVAVAIWGGIYGSFFGYELPFHL
                     ISTTSDVMIILVVSVVFGFITVFAGLLASGLQKVRMKKYAEAYNSGFVWCVILLGLLF
                     IAVGMLMPDMRLLFVLGKWVSIFNAVGILVVSIIQAKSLSGIGAGLFNLYNISSYIGD
                     LVSFTRLMALGLSGASIASAFNLIVGLFPGTLAKLTIGLVLFILLHAINIFLSLLSGY
                     VHGARLIFVEFFGKFYEGGGKPFQPLKASEKYIKVITKN"
     gene            12173..12649
                     /locus_tag="VQ514_000955"
     CDS             12173..12649
                     /locus_tag="VQ514_000955"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HET2499730.1"
                     /translation="MEHLATYFSTYGGAFFAALGIVLAVGLSGMGSAYGVGKAGQSAA
                     ALLKEQPEKFASALILQLLPGTQGLYGFVIGILIWLQLTPELPLEKGVAYFFVALPIA
                     IVGYFSAKHQGNVAVAGMQILAKRPKEFMKGAILAAMVETYAILAFVVSFILTLRV"
     gene            12683..13264
                     /locus_tag="VQ514_000956"
     CDS             12683..13264
                     /locus_tag="VQ514_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499731.1"
                     /translation="MSNLENLRESVIEQAHEKGRMKLLDSKKKIDDEFEMQKSVIVKK
                     KEVEHERKLKELQQKYQIIFQQLKNKERQSTLVSKQKILKELFQSALIEMESWSADKE
                     MEFIYRILERYSQQEVIVTFGERTLAKFNLEQLEKLKFSFPNHLFSEQPISNESGLLI
                     SIGKIDDNYLYKTLIGSISKEESSSIANQIFIN"
     gene            13329..14336
                     /locus_tag="VQ514_000957"
     CDS             13329..14336
                     /locus_tag="VQ514_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HET2499732.1"
                     /translation="MDTNLFSKINTTISVKENDFITEDKFQKIIQSKDTETMAFILEA
                     TPYHLSIDVLENPSQTETSLMTKLVNDYRWAYAESPSNKIVTLFALRYVYHNIKVLLK
                     SKAAIKKDFSKLLIPIGIFDIESLKHLVSSLHSDTLPDFMVHEVESIWNEYETFNNIR
                     VLDVGADLAYFKHLKLLSNELDEVLSQVIVAMIDFYNIITVKRGLSQNKSHGDILQLL
                     SDEGSISAKEFIYIVENQEIFVWFNKINPSLDSIFSTYELKMQDATISSSELEFLCDL
                     LLYKILDQGRYNVDGPLVLARYLLGCEFEVKNLRMIISALQNTIPFGSIKERIRPHYG
                     S"
     gene            14326..14646
                     /locus_tag="VQ514_000958"
     CDS             14326..14646
                     /locus_tag="VQ514_000958"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HET2499733.1"
                     /translation="MEANKYKIGIIGSRDIILPFSMIGFDIFPAYQEQEAINTLRKLA
                     QSDYGVIYITEDIASMILDTIRHYDSQVVPAIILLPTHKQGLNLGLKRIEDNVEKAVG
                     HNIL"
     gene            14709..16484
                     /locus_tag="VQ514_000959"
     CDS             14709..16484
                     /locus_tag="VQ514_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HET2499734.1"
                     /translation="MTQGKIIKVSGPLVIASGMQEANIQDICRVGKLGLIGEIIEMRR
                     DQASIQVYEETSGLGPGEPVVTTGEPLSVELGPGLISQMFDGIQRPLDRFKLATHNDF
                     LVRGVEVPSLDRDIKWHFDSTIAIGQKVSTGDILGTVKETEVVNHKIMVPYGVSGEVV
                     SIASGDFTIDEVVYEIKKLDGSFYKGTLMQKWPVRKARPVSKRLIPEEPLITGQRVID
                     TFFPVTKGGAAAVPGPFGAGKTVVQHQVAKFSNVDIVIYVGCGERGNEMTDVLNEFPE
                     LIDPNTGQSIMQRTVLIANTSNMPVAAREASIYTGITMAEYFRDMGYSVAIMADSTSR
                     WAEALREMSGRLEEMPGDEGYPAYLGSRIAEYYERAGRSQVLGIPEREGTITAIGAVS
                     PPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYSLYKDSVGIYIDGKE
                     KTDWNSKITRAMNYLQRESSLEEIVRLVGIDSLSDNERLTMEIAKQIREDYLQQNAFD
                     SVDTFTSFAKQEAMLSNILTFADQANHALELGSYFTEIMEGTVAVRDRMARSKYVSED
                     RLDEIKIISNEITHQIHLILETGGL"
     gene            16485..17870
                     /locus_tag="VQ514_000960"
     CDS             16485..17870
                     /locus_tag="VQ514_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HET2499735.1"
                     /translation="MSVIKEYRTASEVVGPLMIVEQVNNVSYNELVEIQLHNGEIRRG
                     QVLEIHEDKAMVQLFEGSSGINLEKSKIRFAGHALELAVSEDMVGRIFNGMGKPIDGG
                     PDLIPEKYLDIDGQAINPVSRDYPDEFIQTGISSIDHLNTLVRGQKLPVFSGSGLPHN
                     ELAAQIARQATVLNSDENFAVVFAAMGITFEEAEFFMEELRKTGAIDRSVLFMNLAND
                     PAIERIATPRIALTAAEYLAFEKDMHVLVIMTDMTNYCEALREVSAARREVPGRRGYP
                     GYLYTNLSTLYERAGRLVGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSHE
                     LYNQGYRPPINVLSSLSRLKDKGSGEGKTRGDHAPTMNQLFAAYAQGKKVEELAVVLG
                     ESALSDVDKLYVRFTKRFEEEYINQGFYKNRNIEDTLNLGWELLSILPRTELKRIKDD
                     LLDKYLPLVEV"
     gene            17893..18504
                     /locus_tag="VQ514_000961"
     CDS             17893..18504
                     /locus_tag="VQ514_000961"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HET2499736.1"
                     /translation="MVRLNVKPTRMELNNLKERLKTAERGHKLLKDKRDELMRRFISL
                     IRENNRLRKEVESYLIDNLKSFAVAKSLKNSLMVEELFSIPSKEIELFIEKENIMSVT
                     VPRMHMNITSQNENSEYSYLSSNSEMDDVFATMNSLIDKLLRLAEVEKTCQLMADEIE
                     KTRRRVNGLEYSIIPNLSETIHYIELKLEEAERANLVRIMKVK"
BASE COUNT         6281 a         2640 c         3696 g         5991 t
ORIGIN      
        1 cgatgagtta actatactac agattctcta tggagcgtca ctcatttctt ttttatggat
       61 gtatggtcaa cgaaaacaag tgatcaaggt taatatgaaa actaggatac aatggcttgg
      121 tattggattc gttagcctac tgattataag tctatgtttt agtctaatcc atgcgcaaga
      181 aagcacgaat caagcaaact taattggact tcaacatcaa attccttggt tttcattttt
      241 attgttttta atcaatgcga gtatggttga agaatttctg tatagagaaa ttttatggaa
      301 cttggttaga aaattagata ttcgagttgc tttgacaagt gttttatttg ccttagcaca
      361 tcatccagga accattatag cttggtgttt gtatgtatcg ctggggatgt ttttagggct
      421 tgtacgctac aaattggact tatggggcag tatggggcta catttggtgt ggaacctact
      481 agtttatagt ttgctgcttt tttaaaataa gtgatctgat ttatctcata aatcagtagt
      541 ttttatagga ttatttttaa ttcggaaaca aaaatgtatt aacagcataa aataaagaaa
      601 ctaggaacat tttttttatt ttttagaaat aaaatgttgt atagaaattc cgaaagaaat
      661 aataaaatca aaataaaaat taataaaata ggtatatttg aacatattga cgtcgacaaa
      721 tgttcttgca agacataaaa tacaaataaa aagaataaaa aatttttata tttttatatt
      781 tgttgttctg ttataaaatg aaagaaatta gaaactagtt tctaaatcat gaaaaatatt
      841 gaaaaaaaaa aaaataagtg ttaaaataaa gaatgtaaac gtttacttca ataaaggagc
      901 tcatatgtta ctgcaaaaag aactaattcc aatgatagaa gctaacttac caaatatggc
      961 atatgctgaa aaagacattg ctaaattctt cttaaaacag caacctctga ataattattc
     1021 atgtaaggca ttgtgcgaat gccttaatgt atccaaagca acattgactc gatttgcgaa
     1081 aaaatgtggt tttaaaggtt ttagacaatt cattttcaaa taccaagaga tgattcgtga
     1141 gaaagaaaag ttagcattgt atacagaggc tacagaaaaa gttttatccg actatgaaga
     1201 aatgttgaga aaaacttaca cggttcttga tgaagttcaa cttgagcgta ttgctgagat
     1261 gatagaaact gctgagcgtg tatatcttta tggtaaagta agttctgttc ttgctttaca
     1321 agaaatgaag atgagattta tgcgtctcgg agtcattggt gaagtattat cagacgagga
     1381 tatgattttg tggaatagct tattacttaa tgaaaattgc cttgtcattg gagcatccgt
     1441 ttcaggtcaa actgatattg tactagaagg tcttcaaaaa gctgcggata aaggcgctaa
     1501 aacagtttta atgactacaa gaaaatttga cgaagaagat tgtttctttg atgaactatt
     1561 gttattagct tcgaccaatc atctctcgta tggcaatcgc atatcacctc agtttccaat
     1621 acttttaatt acagactgct tattctctca ttatctggaa agtccagaga gacaatatta
     1681 ttacaatcaa actattatcc ataaggagga ataataaaat gagtcaaatt tggactaaag
     1741 aaaaatttat aagtcaagtc catggtggag tcattgtttc ttgtcaagct ttacccggtg
     1801 aaccccttta taatgaagaa tttagcttga tgccttttat ggctaaagca gctttagagg
     1861 caggagcagt gggcattcgc gcaaattctg tgcgtgatat taaagcaatt cagaaagtag
     1921 tagatttacc aataattgga attatcaaaa gagattatcc acctcaagaa ccatatatta
     1981 ctgctacgat gaaagaagta gatgaacttg tagaatgcgg aacaacagtc attgcatttg
     2041 atgcaacttt aagaccaaga tatgatggct tagttatcaa tgaatttatc aaaaaaataa
     2101 aagaaaaata tcctaatcaa ttgttgatgg cggatgtaag taattttgat gaaggtctct
     2161 atgcatttaa atcaggcgtt gattttgttg gtacaacatt atcagggtac acaagtacaa
     2221 gtgtacaatc agatgagcct gattttgaac taatgaaaaa attggctgat tttaatattc
     2281 cggtaattgc cgaaggaaaa attcattatc cagaacaatt aaaaaaagct tatagtttag
     2341 gtgttaccag tgttgtcatt ggtggagcga ttacacgtcc aaaagaaatt gctcagcgat
     2401 ttattaatgt catcaaataa aaaacaatat aaaggagaaa tctgatgaaa gatttaacta
     2461 aatacaaagg cgttattcct gcattttatg cttgctatga tgaaaatggt gaaattagcc
     2521 aagaacgtgt aaaatctctg gtgcaatatt tcattgacaa aggtgtaaaa ggtatctatg
     2581 taaatggttc ttcaggtgaa tgtatttacc aaagtgtaga agatcgtaaa caaattattg
     2641 aagctgttat ggaagttgct aaaggtaaat taacagttat caaccatgtt gcatgtaata
     2701 acacgaaaga tagtattgaa ttggcaaaac attcagaaag tgttggagtc gatgctattg
     2761 ctgctatccc acctatttac ttcaaattgc cagaatattc aattgcagca tattggaatg
     2821 caatgagtga agctgcgcca aatacagatt ttatcattta taatattcca caattggcag
     2881 gggttgcgtt gactggtagt ttgtatgcaa caatgcgtca aaatcctcgt gtgattggag
     2941 ttaaaaattc ttctatgcct gtacaagata ttcaaatgtt tgtagctgca ggtggagaag
     3001 attacattgt attcaatggt ccagatgaac aatatcttgg tggtcgcttg atgggagcag
     3061 aagctggtat tggtggtact tatggcgtta tgccagattt gttcttgaaa ttggaaagtt
     3121 tgattcaaga acgagattta gatactgcta aaaaacttca atatgctatc aatgaagtta
     3181 tctataagat gatatcaggt aaggcaaata tgtatgctgt agcaaaagag gttttgcgtc
     3241 taaatgaaaa acttgattta ggttctgttc gtcaaccctt agaagcattg gcagaaggtg
     3301 acttggaagt tgcaaaacaa gcagcagaac ttattcaaca agcacgaaaa gaatttttat
     3361 aataaataaa atcaatatgg agatatgtta tgggcacaac aggatttaca ataattgact
     3421 taattatctt gattgtttat ttacttgcgg tgttggttgc aggtatctat ttctctaaaa
     3481 aagagatgaa aggaaaagag ttcttcaaag gagatggttc ggttccttgg tatgttactt
     3541 cggtatccat ttttgccaca atgctcagtc cgatttcctt cttgggactc gctggtaact
     3601 cttatgcagg tagctggatt ttatggtttg ctcaattagg gatggtagta gctattccac
     3661 tgacaattcg ttttatctta cctatctttg cacggataga catcgatacg gcatatgatt
     3721 acttggataa acgttttaat tctaaagcac ttcgtattat ttcagcactc ttgtttatta
     3781 tttatcaatt gggacgtatg tctatcatta tgtacctccc atcagctggt ttatcagtat
     3841 tgacaggaat tgacatcaat attttgatta ttttgatggg tgcagttgca attgtttatt
     3901 cttacactgg tggtctaaaa tccgtattat ggacagactt tattcaaggt gtgattctga
     3961 ttagtggtgt cgttttagct ttatttgtac tgatggctaa tattaaaggt ggctttggtg
     4021 cagtagcaga aacattagca aacgggaaat tccttgctgc aaatgaaaaa cttttcgatc
     4081 ctaacttgct ttcaaactcc atctttttaa ttgtgatggg ttcaggcttt acaatcttgt
     4141 cttcctatgc ttcatctcaa gatttggttc aacgttttac tacaacacaa aatattaaga
     4201 aacttaataa gatgttgttc acaaacggtg tcttgtcact tgcaactgca acagtctttt
     4261 acttgattgg tacaggcttg tacgtattct atcaagtaca aaatgcaggt agtgcagcta
     4321 gcaatatccc tcaagaccaa atctttatgt actttattgc ataccagtta ccagtaggta
     4381 tcacaggttt gattttggca gcgatttatg cagcatctca atcaactatt tcaacaggtt
     4441 tgaactctgt tgcaacttca tggacattgg atattcaaga tgtcatttct aaaaatatgt
     4501 cagacgatcg tcgtacgaaa attgcacaat tcgtatctct agcagtaggt ttattctcaa
     4561 ttggtgtttc cattgtcatg gctcactcag atattaaatc tgcatacgaa tggttcaata
     4621 gcttcatggg acttgtactt ggtctacttg gtggtgtatt tattcttgga tttgtttcta
     4681 aaaaagcaaa taaacaaggt gcttatgcag cgctgattgt atcaactatc gtcatggtat
     4741 ttattaaata cttccttcct ccaacagctg tgagctactg ggcatattca ttgatttcaa
     4801 tctctgtatc agtagtttca ggttatattg tatctgttct tactggaaat aaagtatctg
     4861 cacctaaata tacaacgatt catgatattc cagaaattaa agcggattca agttgggaag
     4921 ttcgtcacta atagaaaaga gaaaataata tgattatttc tgaacaatct gattttagac
     4981 gatatgcttc tatcaataaa catttttcaa aagtctgtga ttttttagaa aatacaaatt
     5041 tgacagattt agttgatgga aaagttgata ttgacgggga aaatgttttt gcaaattgta
     5101 tgatctatct agctgatgga gtcccaggag atatttttga aactcataag aaatatttag
     5161 atattcatat tgttgttgaa aatactgaaa aaatggcagt aacttcccca gttcgtgccc
     5221 agtcacgtgt accttttagc gaagaaaagg atattgcttt ttacgatagc aaagactatc
     5281 aaattgttga attgcttcca gggaatatgt tggtgacttt tgaagaagat cttcaccaac
     5341 caaagattca ttgcaatgat gagcctgtta aaaaacttgt tattaaagtt ttaaacgagg
     5401 aaaaataaaa tgaaaaaaaa tattaaacaa tacgtaacct taggtactgc agtggtatta
     5461 tcagcatttg ttgctaactc agttgcagct caggagactg aaacttctga agtatcaaca
     5521 ccagagttgg tgcaacctgt tgcaccaacg actccgattt cggaagtaca acctaaatcg
     5581 ggtaactctt cggaagttac tgtacaacct agaacagtcg aaactactgt taaggatcca
     5641 tcttctgcaa cggaagaaac tcctgtctta gaaaaaaata atgttacttt aacagggggc
     5701 ggagaaaatg ttactaatga gttaaaggat aaatttacta gcggtgactt tactgtagtg
     5761 attaagtaca atcagtctag tgagaaaggc ttacaagctc tgtttggaat atctaattct
     5821 aaacccggtc aacaaaatag ttatgtagat gtgttcctta gagacaatgg tgaattggga
     5881 atggaagcgc gtgatacttc ttccaataaa aatcacctag tatccagacc tgcttcagtt
     5941 tggggtaagt acaaacaaga agctgtgact aacactgttg cagtagtagc agattcagtc
     6001 aaaaaaacat attctttata tgcaaatggt acaaaagtag tagaaaagaa agtggataac
     6061 ttcttaaaca tcaaggatat taaaggtatt gattactata tgcttggggg agtgaaacgt
     6121 gcaggaaaaa cggcgtttgg ttttaacgga acactagaaa atatcaaatt ctttaatagt
     6181 gcattggatg aagaaactgt taaaaagatg acaacaaacg ctgttactgg acatttaatt
     6241 tatacggcta atgatacaac aggttctaac tatttccgta ttccagttct gtatactttt
     6301 agcaatggtc gggtattttc aagcattgac gctcgttacg gcggaactca tgattttttg
     6361 aataaaatta atattgctac aagttatagt gatgataatg gtaagacatg gactaaacca
     6421 aaattaacat tggcattcga tgattttgcg ccagtaccat tagaatggcc tcgtgatgtt
     6481 ggtggacgtg acttacaaat cagcggtggt gcaacctata ttgactctgt tattgttgaa
     6541 aaaaataaca aacaagtact catgtttgcg gatgtgatgc ctgctggagt aagttttaga
     6601 gaagctacta gaaaagattc aggttataaa caaattgatg gtaattatta ccttaaatta
     6661 aagaaacaag gtgatactga ttacaattat actattcgtg agaatggtac tgtatacgac
     6721 gatcgtacca acagaccaac tgaattttca gtagataaaa atttcggtat taaacaaaat
     6781 ggtaattatt tgacagtaga gcaatattcg gtttcattta aaaataataa aaagactgaa
     6841 tatcgtaatg ggactcacgt ccatatgaat atcttctaca aagatgcatt gttcaaagta
     6901 gtgccaacga actatattgc atatatttct agcaatgatc atggcgaatc ttggtctgca
     6961 ccaactttat tacctcctat aatgggactt aatcggaatg cgccttattt gggtcctgga
     7021 cgtggaatca ttgaaagctc aactggacgt attcttattc cgtcttacac tggtaaagag
     7081 tctgcattca tttatagtga cgataatgga gcatcttgga aagttaaagt tgtgccactt
     7141 ccttctagtt ggtcagcaga agcacaattt gtagaattga gtccaggagt aattcaagca
     7201 tacatgcgta caaataatgg taaaattgca tatttaacaa gtacagacgc aggtactact
     7261 tggagtgcac cggaatattt gaaatttgtt tcaaatccaa gttatggaac acaattatca
     7321 atcatcaatt atagtcaact gattgatggt aaaaaggctg tcattttaag tactccaaac
     7381 tccacaaatg gtcgtaaaca cggacaaatt tggattggtc taattaatga tgataataca
     7441 attgattggc gttatcatca cgacgttgat tatagtaact atggatactc atattcaaca
     7501 ttgacagagt taccaaatca tgaaattgga ttgatgtttg aaaaatttga ttcatggtct
     7561 cgtaatgaac ttcatatgaa aaatgttgta ccatatataa catttaagat tgaagatctg
     7621 aaaaagaatt aaagctgaaa tttgaaaata tataaaaaga ggataaaaat tatggtaaat
     7681 tacggtattg ttggagctgg atattttgga gctgatttag ctcgctcaat gaacaaaatt
     7741 gaagatgcaa aagtggttgc ggtatttgac ccaaatcatg gagaagaagt tgctcaagag
     7801 ttgggatcag atgtttgtgc aagtttagat gaacttgtag cacgtgaaga tattgattgt
     7861 gtgatcgtag cttcacctag ctaccttcac cgtgaaccag ttgtgaaagc tgctcaacat
     7921 ggcaaacacg tattttgtga aaagccaatt gcattgtctt atgaagattg taaagccatg
     7981 gttgacgcat gtaaagaaaa taatgttatc tttatggctg gtcacatcat gaacttcttt
     8041 aacggtgtac accatgctaa agaattgatt actcaaggta aaatcggtaa agttctttat
     8101 tgccatgccg ctcgtacagg ttgggaagaa caacaaccaa ctgtatcatg gaagaaactt
     8161 cgttctcaat ctggaggaca tttgtaccat catattcatg aattagattg cattcagttt
     8221 atcatgggag gacttcctga aaaagcgaca atggtaggag gcaatgtata tcataaaggt
     8281 gaaaactttg gtgatgaaga tgatatgctc attgtaaact tagaatactc tgatgatcgt
     8341 tatgctgttt tggaatatgg taatgctttc cgttggggtg aacactacgt cttgattcaa
     8401 ggaactgaag gagctatcaa acttgatttg ttcaatactg gaggtactct tcgtgttaaa
     8461 ggtgaaggag aatcacactt cttagttcat gaaactcaag aggaagatga tgaccgtaca
     8521 gctatctata ccggtcgtgg tatggatgga gcaattgcgt acggtaaacc aggagtacgt
     8581 tgcccattat ggttgcaaac atgtattgat aaagaaatgg aatatctaca tgacatcatt
     8641 aaaggtggag aaattacaga agaatatgaa aaacttctca atggtgtagc tgctttagaa
     8701 tcaatcgcta ccgctgatgc atgtacttta tcagttaaag aagatcgaaa agtaagtctt
     8761 tcagaaatca caaatgctta acttttgtaa aacagaatag taaattctta tcattatata
     8821 atttctaaag ttctgtgata caactcattg aataaagaaa tagagatggg actgggataa
     8881 tgcccagtcc cattttttat caaaaagtaa tgagatcaaa aatgtgggag tgatgaaatg
     8941 aagattatag ggatcgatat tggcggaaca acaattaaag cagatttata caatgagttt
     9001 ggaacgagtt taaatcagtt caaagagata gaaacaatta ttgactatga tttgggaacg
     9061 aaccagatat taaatcaggt ctgtgattta attggtgagt atattttaaa tcattcaatt
     9121 gatggtgttg ggatttccac tgctggagtt gttaatgcta atacaggaga gatcatctat
     9181 acaggctata caataccagg gtatatcgga gtaaacttta ctttcgaaat agaaaaacgt
     9241 tttgggttgt ctacttttgt tgaaaatgat gttaattgtg ctgcattagg tgaattgtgg
     9301 aagggacaag ccaaagataa gaaaaatgta gtaatggtta ctattggaac aggtatagga
     9361 ggcagtatta ttgtcaatgg acaaattgtt aacggattta actatactgc tggtgaagta
     9421 ggctatattc ctgtaggtaa ttcggattgg caaagcaaag cctcaacaac cgcattgatt
     9481 catttatatc aaaagaaaag cttgaaaact aatcaaactg ggcgtacttt ctttactgat
     9541 ttaagagctg gagataaagt cgctaaagaa acttttgaaa tttttgtaga aaatctaaca
     9601 aaaggtttat taacgattac ttatctactt aatccagaaa ttctcatatt aggaggtggg
     9661 atcctggcta aaaaggatat tttgttacct gaaattcaaa gttctttagc taaaaatgca
     9721 atggataata ggtttttgcc taaaaatctt gtggcagcta cattaggaaa tgaagctggt
     9781 cgtataggag ctgtaaaaaa tttcttagat agaatttcta ataaatagta tgaaagataa
     9841 ggaggtgtca caatgactaa ctctgtattt tcgacaatgc aagatattga gaatgttgca
     9901 accgatatta taaaatcata tgataatgag atttatactt ataaagctgt ttcccaagaa
     9961 gaattggaaa aactagaaaa aagttatgat gaaaaaagtc acgaagaatt agtttcaata
    10021 gaaagcaatt tagaaatgaa acaacagaac cttattgatg aggttaataa aacaatcaag
    10081 gaaaattatg aaaagattca gtatatttca tcaagtagga gaggagaatt tgtagaaaaa
    10141 attattggta gggtggtaga aaaatatggc tattagtcag atgaaaagaa tttctctact
    10201 attttctaaa agtagtcttg atgatgtttt aaaaactatt caagaactag agtcagtgca
    10261 gttccgtgat ttaaaggttc aggataactg gtcagaagct ctagaaaaag atgaggttgt
    10321 atttccaact attcaaattt ctcatacttc taattccaat catggggtta ttgaaggaaa
    10381 tgatgccttg atttatttga tgaatcaaca acaatattta gaagcaactg tagagaaatt
    10441 acaagaatac ctaccgaaag aaaacacgtt taaattatta cagcaacctc cgataacgac
    10501 ctcttatgaa gaattagaaa aatttggtaa agctaatgtt gctgagggtg ttcttaaaaa
    10561 agtgaatcgt caaataaaca gagttcatga attagaaaga cacattcaaa gtaataatga
    10621 ggaaatagag agattaataa aatgggaaaa attagaaatt attcctgcgc atttagaaca
    10681 attttttttc tgtaaaggaa aagtcggaac aattccaagg actgtagata atcgcttata
    10741 caatagtttt ttagaaaaca atattgaagt tcaagaaata ttttctaatg atagagagta
    10801 cggtgttgtt gttttctatc agtccagttg ctctattgat tttgatgaat acttatttga
    10861 accatttgat tattctagaa aggaattacc gaagcagcga gtagtagatt tggatcaaga
    10921 aaacatgcag ttaataactg aaaaagagaa tattatcgca tcgttgcaag attcaaagaa
    10981 taatttgata gatttacaat ggcaaattga ctatatttta tctatctatg ctcgtcaaat
    11041 ctctaagaat aaccttttga gcactccgca tctagttgca ctagaaggat ggatagaaga
    11101 aactcgtatt ttatatttta taaaagttat ggatgagcgt tttggacatt ctatttatat
    11161 ttatgaatcg gaaacattgt cggataatca agatgaaata cctatcaaat taacgaatca
    11221 ttctttaatt gaaccatttg aattattgac agaaatgtat gctctgccca aatattatga
    11281 gaaagatcct acacctgtat tagcaccatt ttactttaca ttttttggaa tgatggttgc
    11341 tgatttaggc tatggtttac tattgttttt aggaacaatg ttagcattaa aaatttttca
    11401 tctaccttca gaaactaaga gatttttaaa attctttaat atattagggg tagccgttgc
    11461 aatttggggt ggaatctatg gctcattttt tggatatgag ttgccatttc atctgatatc
    11521 tacaacctct gatgtcatga ttatattagt agtgtcagtt gtgtttgggt ttattacagt
    11581 atttgcaggt ttgttagctt caggactaca aaaagtaaga atgaagaaat atgcagaagc
    11641 atataattca ggatttgtgt ggtgtgttat tctgcttggc ttgttattta ttgctgtcgg
    11701 aatgttgatg cctgatatga gactgttatt tgtattaggg aaatgggtat ctatttttaa
    11761 tgctgtggga attttggttg tttctattat tcaagccaaa agcttgtcag gtattggagc
    11821 aggattgttt aatctatata acatttcatc ttatataggt gatttagtta gtttcactcg
    11881 attgatggca ttaggattat ctggagcaag tatagcatca gctttcaatt taattgttgg
    11941 tttgtttccg ggaacattgg ctaaactaac aattggatta gtattattca ttcttttaca
    12001 tgcgatcaat atttttctat cgttactatc aggatatgtt catggagcac gtctgatatt
    12061 tgttgaattt tttggtaagt tttatgaggg tggaggaaaa ccatttcaac ctttgaaggc
    12121 ttctgagaaa tatattaagg ttattacaaa gaattaatgg aggatatata taatggaaca
    12181 tttagcaact tatttttcaa cctatggagg agctttcttc gctgcattgg gaattgtatt
    12241 ggcggttgga ttaagtggta tggggtctgc ttatggagtt ggtaaggctg ggcaatctgc
    12301 cgcagcttta ctgaaagaac agccagaaaa gtttgcctct gctttgatat tgcaattact
    12361 gcccggaaca caaggattat atggttttgt tattggaatt ttgatttggt tgcaattaac
    12421 tccagaactt cctttagaaa aaggcgttgc ttatttcttt gtagctcttc caattgctat
    12481 tgtaggatac ttttcggcta agcatcaagg aaatgtagca gtagcgggaa tgcaaatctt
    12541 ggctaaaaga ccaaaagaat tcatgaaggg agcaattttg gctgccatgg tagaaaccta
    12601 tgcaattctt gcctttgtcg tatcattcat tttgaccctt cgtgtataag aaattaattt
    12661 gctattcaaa ggaggtgtct aaatgagcaa tttagaaaac ttacgagagt ctgttattga
    12721 acaagctcat gaaaaagggc gtatgaaatt attggattcc aaaaagaaga ttgatgatga
    12781 atttgaaatg caaaagtcgg tcattgtaaa gaaaaaagaa gtggaacatg aacgaaagtt
    12841 aaaagaatta caacagaaat atcaaataat ttttcaacaa ttaaaaaata aggaacgcca
    12901 atcaacgtta gtatcaaaac agaaaatatt aaaagaactt tttcaatctg ctttaataga
    12961 aatggaatct tggagtgcag ataaagaaat ggagttcatc tatcgaattc tggaacgata
    13021 ttcacaacaa gaggtcatag taacttttgg ggagcggact ttagctaaat tcaatctgga
    13081 acaattagag aaattgaaat tctcttttcc aaatcattta tttagtgaac agcctatctc
    13141 aaatgaatca ggcttactta tttcaatagg taaaattgat gataactatt tgtataaaac
    13201 attaattgga tcgatttcta aggaagaaag ttcaagtatc gcaaatcaaa tttttatcaa
    13261 ttaaggatga aattggttaa tccttcttag aaatttggag tattccaata aaattagaaa
    13321 ggtattttat ggatactaat cttttttcaa aaataaatac gacgatttcg gtaaaagaaa
    13381 acgattttat tacagaagat aaatttcaaa aaattataca atccaaagat acggagacaa
    13441 tggcatttat cttagaagca actccctatc atttatcgat tgacgtttta gaaaatccta
    13501 gtcagacaga gacttcgcta atgacaaaat tagtcaatga ttatagatgg gcctatgctg
    13561 aaagtccgtc taataaaatt gtgactttat ttgctttacg atatgtttat cataatatca
    13621 aagttttatt aaaatctaag gcggcaatta agaaagattt ttctaaatta ttaattccaa
    13681 tagggatttt tgatatagaa agtttaaaac atttagtttc ttccttacat tcagatacac
    13741 ttcctgattt tatggttcat gaagtagaat caatttggaa tgagtatgaa acttttaata
    13801 atattcgtgt acttgatgtc ggagctgatc tagcatattt taaacatctg aaacttttat
    13861 ctaatgagtt agatgaggtg ctgtctcagg ttattgtcgc aatgattgac ttttataata
    13921 ttattactgt aaaacgtggt ttatctcaaa ataagagtca tggggatatt ttacaattac
    13981 tttcagatga aggaagtatt tctgctaaag aatttatata cattgtagaa aatcaagaaa
    14041 tatttgtgtg gttcaataaa ataaatccaa gcttagattc aatcttttca acttatgaat
    14101 tgaagatgca ggacgcaaca atttcatctt ctgagttaga atttttatgt gatttattat
    14161 tgtataaaat tttagatcaa ggaaggtaca atgtagatgg gccgttagta cttgctagat
    14221 atttattggg atgtgagttt gaagtaaaga atctcagaat gattatatca gctcttcaaa
    14281 atacaattcc ctttggatca ataaaagaaa ggatacgccc gcattatgga agctaataag
    14341 tataaaattg gcataattgg tagccgtgat attattttac cctttagcat gattgggttt
    14401 gatatatttc ctgcctatca agaacaagaa gctataaata ctctaagaaa attagcccaa
    14461 tctgattatg gtgtcattta tatcactgaa gacattgctt caatgatatt agatacaatt
    14521 cgccattatg attctcaagt tgtgcctgct attattttat taccgactca taaacaaggt
    14581 ttaaatttag gattaaaacg tatagaggat aatgtggaga aagcagtagg acacaatatt
    14641 ttataataat gtacaaaatt gtctgtaata ttattctata atttttggac ttagtaagga
    14701 gaataacttt gactcaaggg aagattataa aagtatcggg acctctagtt attgcatcag
    14761 gtatgcagga ggctaatatt caagatattt gccgtgtagg taagctaggg ttaatcggtg
    14821 aaattattga aatgagaaga gatcaggcat ctatccaagt ctatgaggaa acatctggtc
    14881 ttggtccggg agaacctgtt gttacaactg gagaacctct ctcggttgaa ttaggaccag
    14941 gattgatttc tcaaatgttt gatggcatac aacgcccatt agatcgattt aaattggcta
    15001 ctcataatga ttttctagtt cgtggggtag aagttccaag tttggataga gatattaagt
    15061 ggcattttga ttccactata gcaattggtc aaaaagtgag tacgggtgat attctaggaa
    15121 ctgtcaagga aaccgaggta gttaatcata aaattatggt tccttatgga gtatctggcg
    15181 aagtcgtttc tatcgcatct ggcgatttta caattgatga agttgtatat gaaataaaaa
    15241 aattggacgg tagtttctat aaaggaacgc ttatgcaaaa atggcctgtt cgcaaggcgc
    15301 gtcctgtttc taaacgttta atcccagaag aaccattaat cacaggtcaa cgagttattg
    15361 atacattctt tccagtaacc aaagggggag cagcagctgt tcctggaccg tttggagcag
    15421 gaaagacagt tgtacaacac caagtagcta aattttccaa tgttgatatt gttatttatg
    15481 tcggttgtgg agaacgtgga aatgaaatga cggatgtact gaatgagttt cctgagttga
    15541 ttgaccctaa taccggacaa tcaattatgc aacggacagt tctgattgct aatacttcaa
    15601 atatgcctgt tgctgctcgt gaggcttcaa tttatacagg aattaccatg gctgagtatt
    15661 ttcgtgatat gggctactct gtcgccatta tggctgattc gacttcacgt tgggcagaag
    15721 cactacgtga aatgtcagga cgtctagaag aaatgcctgg tgatgagggt tatcctgctt
    15781 atctgggaag tcgtatcgct gaatattatg aaagagcggg acgttctcag gttctaggga
    15841 ttccagaacg tgaaggaact attactgcta ttggagctgt atcgccaccc ggtggagata
    15901 tttcagaacc agttactcaa aacactttac ggattgtgaa agttttttgg gggcttgatg
    15961 ctccgttggc acagagacgt cattttcctg caattaactg gcttacatct tattcactct
    16021 ataaagacag tgtgggaatt tatatagatg gtaaagagaa gacagattgg aatagtaaaa
    16081 taactcgtgc gatgaactac ttacaacggg aatctagttt agaggaaatt gttcgtcttg
    16141 ttggaattga ttctctgtct gataatgaac gactaacgat ggaaattgct aaacaaattc
    16201 gagaagatta tttgcaacag aatgcttttg attcagtaga tacattcact tcgtttgcaa
    16261 aacaagaagc aatgctaagt aatatcctaa cttttgctga tcaggcaaat catgctttag
    16321 agttaggttc ttactttaca gagattatgg aaggtaccgt ggcagttcga gaccgtatgg
    16381 cgcgaagtaa atatgtttca gaagatagat tagatgaaat caaaattatt tcaaatgaga
    16441 ttacacatca aattcatttg atattagaaa caggaggtct ataaatgagt gttataaaag
    16501 aatacagaac tgctagtgaa gttgttgggc ctcttatgat tgttgaacaa gtaaataatg
    16561 tgtcttacaa tgagttagtt gaaattcaac ttcataatgg agaaattcgt cgtggacaag
    16621 ttttagagat tcacgaagat aaagcaatgg ttcaactttt tgaaggatct agtggaataa
    16681 atttagaaaa gtctaaaatt cgttttgctg gtcatgcact agaattggct gtatctgagg
    16741 atatggttgg tcgtattttt aatgggatgg gaaaaccaat tgatggtgga ccagatttaa
    16801 ttccagagaa atatttagat attgatggtc aagctattaa tcctgtatct agagattatc
    16861 cagatgaatt tattcagaca gggatctcct ctattgatca tttgaatact cttgtacgtg
    16921 gtcaaaaatt accagtattt tcaggttcgg gcttacctca taatgaatta gcagctcaga
    16981 tagcaagaca agcgactgtt ttaaattctg atgaaaattt tgcggttgta tttgcagcaa
    17041 tgggtattac ttttgaagaa gctgagtttt ttatggaaga actcagaaaa acaggagcga
    17101 tcgatcgttc agtcttattt atgaacttgg caaatgatcc tgcaattgag cgtattgcaa
    17161 ctccccgcat tgctttaacc gcggcagagt atctagcttt tgaaaaagat atgcacgttc
    17221 tagttatcat gacggatatg actaactatt gtgaagcgtt acgtgaagtc tcggcagcgc
    17281 gccgtgaagt tccaggtaga cgaggctatc cgggatattt atatacaaat ttatcaactc
    17341 tatatgaaag ggctggtcgc ttagttggta aaaaaggttc agtgacacag attcctattt
    17401 taacaatgcc agaagatgac ataacacatc caattcctga tttaactgga tacattactg
    17461 aagggcaaat tattttgtct catgagttgt ataatcaagg ttatcgtcca ccaatcaatg
    17521 ttttatcttc tctctctcga ttaaaagata agggatctgg agaaggtaaa actcgtggag
    17581 atcatgctcc aactatgaat caactgtttg cagcctatgc ccaagggaaa aaggttgaag
    17641 agttagcagt agtattagga gaatcggctt tatctgatgt agataaattg tatgtgagat
    17701 ttacaaagcg ttttgaagaa gagtacatca accaaggatt ttataaaaat cgaaatatag
    17761 aagatacgtt gaatcttggg tgggaattac tatcaattct tcctagaaca gagttaaaac
    17821 gtatcaaaga tgatttgctt gataaatatt tacctttggt agaagtttaa tccagaaatg
    17881 gagtgattat ctatggtacg tttgaatgta aaaccaactc gtatggaatt gaataactta
    17941 aaggaacgtt tgaaaacagc tgaacgtgga cataagttat taaaggataa aagagatgaa
    18001 ttgatgaggc gatttatttc tttgattcgt gagaataatc gacttcggaa agaagtagaa
    18061 agttatctaa ttgataatct aaaatctttt gcagttgcta aatcattaaa gaactctcta
    18121 atggtggagg aattattttc aattccatcg aaagaaattg aattatttat tgagaaagaa
    18181 aatatcatga gtgtaacagt tcctagaatg catatgaata ttacttctca aaatgagaac
    18241 agtgaataca gctatttatc ttctaatagt gaaatggatg atgtatttgc tacaatgaat
    18301 agtttaattg ataaattact aagactggca gaagttgaaa aaacgtgtca gttaatggct
    18361 gatgaaatag aaaaaacacg tagacgtgta aatggtttag aatattcgat tattccaaac
    18421 ttgtcggaaa ctattcatta tatagaattg aaactagagg aggcagaaag agccaattta
    18481 gttcgtatta tgaaagtgaa gtagttcctt tatttagatt tttaattaga tgaacaaata
    18541 tcagcttgga taaggcggta agcctttcta agctttttta ggctctttgt caactgtagt
    18601 gggttgaa
//
LOCUS       DAVBCW010000033        17806 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVBCW010000033 DAVBCW010000000
VERSION     DAVBCW010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17806
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.033"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(166..780)
                     /locus_tag="VQ514_000962"
     CDS             complement(166..780)
                     /locus_tag="VQ514_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HET2499737.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(1595..1864)
                     /gene="rpsO"
                     /locus_tag="VQ514_000963"
     CDS             complement(1595..1864)
                     /gene="rpsO"
                     /locus_tag="VQ514_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HET2499738.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            complement(2090..2635)
                     /locus_tag="VQ514_000964"
     CDS             complement(2090..2635)
                     /locus_tag="VQ514_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001852743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET2499739.1"
                     /translation="MKLAVIAANGQAGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGSGS
                     LYIDETKTTRLLDTPDFPEEFKSLAKAQADELDLLRTKNNLNWAFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKRNKPD"
     gene            2874..3074
                     /locus_tag="VQ514_000965"
     CDS             2874..3074
                     /locus_tag="VQ514_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499740.1"
                     /translation="MRRFYSHLPYYLVILFFYWPLYELFLLVVSDPLTLKGLYINNLL
                     FFTPMVILIVSLLYSYRFRFSL"
     gene            3505..3645
                     /locus_tag="VQ514_000966"
     CDS             3505..3645
                     /locus_tag="VQ514_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HET2499741.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDFKWTVEEDLLALSNDIGN
                     FQ"
     gene            3652..3828
                     /locus_tag="VQ514_000967"
                     /pseudo
     CDS             3652..3828
                     /locus_tag="VQ514_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
     gene            3886..5829
                     /gene="thrS"
                     /locus_tag="VQ514_000968"
     CDS             3886..5829
                     /gene="thrS"
                     /locus_tag="VQ514_000968"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HET2499742.1"
                     /translation="MINITFPDGAIREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHGDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(5878..6852)
                     /locus_tag="VQ514_000969"
     CDS             complement(5878..6852)
                     /locus_tag="VQ514_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HET2499743.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVEMQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLLLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(6845..7522)
                     /locus_tag="VQ514_000970"
     CDS             complement(6845..7522)
                     /locus_tag="VQ514_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2499744.1"
                     /translation="MHKILLIEDDQVIRQQIGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            7678..8751
                     /locus_tag="VQ514_000971"
     CDS             7678..8751
                     /locus_tag="VQ514_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HET2499745.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPREPTMLTSKNRLQLLDKLAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHKIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSQQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILEAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYQAWKNR"
     gene            complement(8842..9144)
                     /locus_tag="VQ514_000972"
     CDS             complement(8842..9144)
                     /locus_tag="VQ514_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499746.1"
                     /translation="MYLVIGIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(9212..9649)
                     /locus_tag="VQ514_000973"
     CDS             complement(9212..9649)
                     /locus_tag="VQ514_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET2499747.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKKSKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELTSVARGTGGTDIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            complement(9725..10567)
                     /locus_tag="VQ514_000974"
     CDS             complement(9725..10567)
                     /locus_tag="VQ514_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET2499748.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQLGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            10679..11329
                     /locus_tag="VQ514_000975"
     CDS             10679..11329
                     /locus_tag="VQ514_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HET2499749.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRMHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            11539..12173
                     /locus_tag="VQ514_000976"
                     /pseudo
     CDS             11539..12173
                     /locus_tag="VQ514_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
     gene            12304..12741
                     /locus_tag="VQ514_000977"
     CDS             12304..12741
                     /locus_tag="VQ514_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499750.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFITRLETSFEWLLLSGLIFFLA
                     QGLLLFLLVVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMP
                     IITACHSTFYYMTADYLRENYPDFYDKHISNTYTILSISSFLV"
     gene            complement(12836..13135)
                     /locus_tag="VQ514_000978"
     CDS             complement(12836..13135)
                     /locus_tag="VQ514_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499751.1"
                     /translation="MNRNVLNFLRTESAERVSLYIDKANRLEGDVTLLAPSSQDLEDI
                     KNAMFSNPNLELKVARLDVMKKIAYASTRNHYLTGATIFGDISKGTYNCDPKSYV"
     gene            complement(13543..13905)
                     /locus_tag="VQ514_000979"
     CDS             complement(13543..13905)
                     /locus_tag="VQ514_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499752.1"
                     /translation="MRLASESRQILDKLKDDVYNKLGYEVSYSSIVSQAVREYVPKKE
                     RIDWIKLKETAIPFSSLKQSNNWEYQTSLMLEKDVLILLSELQNFFLDVFQAKRIHRA
                     FCIRLCLRAQFLLSNNDS"
     gene            14264..14678
                     /locus_tag="VQ514_000980"
                     /pseudo
     CDS             14264..14678
                     /locus_tag="VQ514_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720030.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14717..15160)
                     /locus_tag="VQ514_000981"
     CDS             complement(14717..15160)
                     /locus_tag="VQ514_000981"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HET2499753.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKKGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(15190..17412)
                     /locus_tag="VQ514_000982"
     CDS             complement(15190..17412)
                     /locus_tag="VQ514_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HET2499754.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRMIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
BASE COUNT         5338 a         3952 c         3156 g         5360 t
ORIGIN      
        1 ttcttatttc attttactat atttataaga atctcctaat caaatcaaaa gccactctac
       61 aatagagtaa ctttaagatt gtccatttat actcaatgaa aaagccctct gaaaatcaga
      121 gagctgattt gagctcggac taaaatccta gtgaaacaaa aaaatctaca cgatcagaaa
      181 agtctctatc gtgccatttt catacatgat gtatagtcca agtagaatga atactaaggg
      241 cacaatgatt cgctcgtatt tttcaattgt ctcgaatatt aacggaatag aagataacac
      301 ccgactaatc tcgcaaaaga taattatgcc gattacaaac acaagcaagg ccacgagggt
      361 ctgtgaccaa tctaacgaag caaaataagg tatatagata cctaaattat ctccgccaga
      421 cgcaattgtc agcaatgtaa ctgtccaaaa cagttgattt gccttgcttt gttctaatct
      481 ttcaataatt tcttcctctt cttcttccgc atcttctcca acaattgcaa agcgaatccc
      541 taaatagata gggattaaac caagcaatcc aaccatccat tcttcaggca cgaaattaac
      601 gacataagca gcaactaaac tcgcccctac aagtaagcct gtgcctagat attgccccgc
      661 ataaatatgc catttctgtt tattctgtga tagctgtgca aataaaataa ttaaaataat
      721 taaataatcg atactggtgg aaatataaac accaatagca gatatgattg tctgtcccat
      781 aaaaaaaact cctgtattag tcagtaataa atcagcagat tttaggagag cacagacaca
      841 ttaatttagc tatcgctagt gggtaatatc gaacgtagga aagcatttta ctcctcctca
      901 aaacgtagtt acaaagtaat ttctaactgg aattcagtga ctacttccat gtaaagtata
      961 acatactata ctttactttg taaagagtgg gctcttctag ggggcttgca tacaactact
     1021 cggcttgaaa agccgagtaa ctgcgcacct ttatggtgta attagctgat accgtttgag
     1081 gtttttgtag tctccaaaat tgttaccgat aaaacctcaa taaaaaagcc ctctgaagtc
     1141 agagagctga tttgagtccg ggctaaaatc ctagtgaaaa agatgaaact ccttgtgctc
     1201 atcgaacact gtgtcatttc cctattttca tacggatttt tgacgccctt agcatcatga
     1261 ttcttgctag ataaatcgtt tatagactag gcgacgaggc gaagatcgta cgaaactttc
     1321 agtgaaacaa aaaaaatctc cccgaaggga gaaaagctgg tttattttac cttacagtgc
     1381 taggaaccgt aaccaaagat tatacttgag tatatcgagg taaggtgaca acacaaaaaa
     1441 gctacccaat ttagagtagc ttcattatca agatatgctc aattgaacac ggcctaagca
     1501 cttggcaaaa aagataaaat ctcctagaaa ctgaagtttc ttcgtaaatt ttcctatttt
     1561 tactttgtgc ttttgacagc ctttgtatct tgaattagcg acgaagtcct agagagttga
     1621 tcaactcacg gtaacggtta acgtcgtttt tacgcaagta tgcaagcaag ttacgacggc
     1681 gaccgatttt tttcatcaat ccacggtaag tagcgtggtc ttttttgtgt tgtttgatgt
     1741 gttcattaag gtggttgatt tcccaagtaa ggacagcaac ttgaacctct actgaacctg
     1801 tatcaccttc gtgacgtgca tattgtgcga tgatttcatt ttttttctct tttgagattg
     1861 ccatgatgtt tctccttttt atttggcttc atccgagtga caggttggca tgcctgtaac
     1921 caagaagaag ttatttgtct ttacgacaat ccctattcta ccaagattta aattttctgt
     1981 caaacattaa tactctcata gttattattt ttcaagtaaa gagatacgtt cttttatatg
     2041 atgacctttt tcaagttcat ctaccaatcc aatagcataa tctgcataac taatctggct
     2101 tattcctttt ttcattagta gtaaaaattt ctcctgctag aatgtagttt cctgtttttt
     2161 caccatcggg aataaaatct acagcaggac tgacaaaagc ccagttgaga ttattttttg
     2221 tacgaagaag atccaattca tccgcttgag cttttgctag tgacttaaat tcttcaggaa
     2281 aatcaggcgt atctaaaagt cgtgtcgttt ttgtttcatc tatataaagg cttcctgatc
     2341 cccctacaac tagaaaacgt gtctgtgttc cagcaagaag ctgattaaat agttcgattg
     2401 atttgctgtg gagcggtagc gtatctggtg tataagcacc aaacgctgaa ataacagcat
     2461 caaatccagt aagatcatct tttgtcaact caaataaatc ttttttaata atagactcag
     2521 cttgactttt gttttcagaa cgaacaatag ccgttacttc atgtcctcgt ttgactgctt
     2581 cttcaacaat tgccttcccc gcttgtccat ttgctgcaat aactgctagt ttcatttttt
     2641 atacccctct tgttgtaatt attttagtta caacaattgt gacactctta ataatcaatg
     2701 tcaatagtct tgcttaatta ttatcaaaat atttctacca agaaaactaa ccatgattct
     2761 agtgaaaaga aaaatcttct ttgtcaacaa atttactttc ttgttttaaa catactataa
     2821 taatcatagc aagagatcta agttgtctgt ttttttaaaa cgaggtgatt atcatgcgta
     2881 gattctattc ccatctcccc tactatctgg tcatattatt cttttattgg ccactttatg
     2941 agttgttctt gctagttgtt tctgaccccc ttacactcaa gggactctat ataaacaatc
     3001 ttctcttctt tacacctatg gtaatcttga ttgtatcgtt actctatagc taccgtttcc
     3061 gtttctcact ttgatggtta gttggtaacg gactgctctt ttactttact atcataacct
     3121 ttggtgtgtt tatactaatt tacttgctaa tctatgaaac agttgctctg gtgggcatgg
     3181 attctggtat tagcatcaag catattctac aaaaaatgaa aaacaaaaaa ctttcacaaa
     3241 atccttgaaa aatctcacaa tcatgctata ataatccata gagacaagtc acttagtccc
     3301 tttctactag agagtgcgtg gttgctggaa acgcatagga agtctaaact gatactactc
     3361 ttgagttttt tatgaaaaca taaaacggtg gccacgttag agccgatcag aggtgtccct
     3421 ctcttttgag gtacataaat gaaggtggaa ccacgttgcg acgtcctttc gaggatgtcg
     3481 cattttttta ttaggatact aattatggag ttgcaagaat tagtggagcg cagttgggca
     3541 atccgacaag cttatcacga actggaagtt aagcatcatg atttcaagtg gacggtagaa
     3601 gaagacctct tggctttatc taatgatatt ggaaatttcc aatgactggt gatgacaaag
     3661 caaggacgct actatgatga aacaccctac acactggaac aaaaactttc agaaaatatc
     3721 tggtgactat tagaactttc tcaacgtttg gatatagata ttctgacgga aatggaaaac
     3781 ttcctctctg ataaagaaaa gcaattgaac gttagaactt ggaagtagtc tgctgataaa
     3841 aaatcaatgc ttagaaacta tgaaataata aaaaaggaga acatcatgat taacattact
     3901 ttcccagatg gcgctattcg tgaattcgaa tctggcgtaa caacttttga aattgcccaa
     3961 tctatcagca attccctagc taaaaaagcc ttggctggta aattcaacgg caaactcatc
     4021 gacactactc gcgctatcac tgaagatgga agcatcgaaa ttgtgacacc tgatcacgga
     4081 gatgcccttc caatcttgcg tcactcagca gctcacttgt tcgcccaagc agctcgtcgt
     4141 cttttcccag acattcactt gggagttggt ccagccatcg aagatggttt ctactacgat
     4201 actgacaaca cagctggtca aatctctaac gaagaccttc ctcgtatcga agaagaaatg
     4261 caaaaaatcg tcaaagaaaa cttcccatct attcgtgaag aagtgactaa agacgaggca
     4321 cgtgaaatct tcaaaaatga cccttacaag ttggaattga ttgaagaaca ctcagaagac
     4381 gaaggcggtt tgactatcta tcgtcagggt gaatatgtag acctctgccg tggacctcac
     4441 gttccatcaa caggtcgtat ccaaatcttc caccttctcc atgtagctgg tgcgtactgg
     4501 cgtggaaaca gcgacaacgc tatgatgcaa cgtatctacg gtacagcttg gtttgacaag
     4561 aaagacttga aaaactacct tcaaatgcgt gaagaagcta aggaacgtga ccaccgtaaa
     4621 cttggtaaag agcttgacct ctttatgatt tcacaagaag tgggacaagg tttgccattc
     4681 tggttgccaa atggtgcgac tatccgtcgt gaattggaac gctacatcgt aaacaaagag
     4741 ttggcttctg gctaccaaca cgtctacact ccaccacttg cttctgttga gctttacaag
     4801 acttctggtc actgggatca ttaccaagaa gacatgttcc caaccatgga catgggtgac
     4861 ggggaagaat ttgtccttcg tccaatgaac tgtccgcacc acatccaagt tttcaaacac
     4921 catgttcact cttaccgtga attgccaatc cgtatcgctg aaatcggtat gatgcaccgt
     4981 tacgaaaaat ctggtgccct cactggcctt caacgtgtac gtgaaatgtc actcaacgac
     5041 ggtcacctat tcgttactcc agaacaaatc caagaagaat tccaacgtgc ccttcagttg
     5101 attatcgatg tttatgaaga cttcaacttg actgactacc gcttccgcct ctctcttcgt
     5161 gaccctcaag atactcataa gtactttgat aacgatgaga tgtgggaaaa tgcccaaacc
     5221 atgcttcgtg cagctcttga tgaaatgggc gtggactact ttgaagccga aggtgaagca
     5281 gccttctacg gaccaaaatt ggatatccag attaaaactg cccttggaaa agaagaaacc
     5341 ctttctacta tccaacttga tttcttgttg ccagaacgct tcgacctcaa atacatcgga
     5401 gctgatggcg aagatcaccg tccagtcatg atccaccgtg gggttatctc aactatggaa
     5461 cgcttcacag ctatcttgat tgagaactac aagggggcct tcccaacatg gctggcacca
     5521 caccaagtaa ccctcatccc agtatctaac gaaaaacacg tggactacgc ttgggaagta
     5581 gccaagaaac tccgtgaccg cggtgtccgt gcagacgtag atgagcgcaa tgaaaaaatg
     5641 cagttcaaga tccgtgcttc acaaaccagc aagattcctt accaattaat tgttggagac
     5701 aaagaaatgg aagacgaaac agtcaacgtt cgtcgctacg gccaaaaaga aacacaaact
     5761 gtctcagttg ataattttgt tcaagctatc ctagctgata tcgccaacaa atcacgcgtt
     5821 gagaaataag agtctagcat aaaagcctcc aatctggagg ctttttctca tctattttta
     5881 ctcaaggact aagttcactt gagcaaactg aatccgcact gtcgttcctt ttccgacctc
     5941 agactcgata cgaatctggt gccccagttc ttcagaaatt ttcttagata gataaaggcc
     6001 aagtccagag gactgctggg tcaaacggcc attgtatcct gaaaagccac gttcaaatac
     6061 tcggaggaca tcactgtttt ttatcccgat tcccgtatct ttgatacaaa gctcttggtc
     6121 atccatataa atctccagac caccttcctt ggtgtacttg agactgtttg agatgatttg
     6181 ctcaataacc actagcagcc actttttatc cgtcacgatt tctttatcaa ggtcatgtag
     6241 attgacattt aagccttttt gaataaagaa aagagcatat ttacgaatta tttccttgac
     6301 caagtcctca atttgaacct gctttaagag caaatcatca tggaaacttt ctaaacgcag
     6361 gtactgtaaa actaggttgg tataagagtc gattttgaaa atttcctgtt ctagctgctg
     6421 cttcagttgg cggtcgacca cttctgcaac taagagttga ctggctgcaa tgggggtctt
     6481 tatctgatgg acccacaagg tatagtaatc cagcaaatcc gtcagttttc tttctgcttt
     6541 tgacctctgc tgatagagtt ccatctcacg cgcttctaat ttttctgcta aagctatttc
     6601 caaaggagac ttggcttccc tctctccata gagaagttcc tggcgataga cctgcatttc
     6661 caccaatatg tcccaagtga aaaataagat ggttacaaag caacacaaga agaaaaagta
     6721 gaggaagtaa attcctagac tggcaaataa aaactgaaag agtaagacaa gaaatgccaa
     6781 agaaagcaga tagataaaaa gacgactacg ggagcgcaga taggctagaa aaaattgttt
     6841 ccaatcaagc atgcttcaat ccgtacccta ttcctttctt ggtctcgata aatcctacca
     6901 atccctgctc ctccaacttt ttacgcaaac gagccacatt gacagagagg gtattatcat
     6961 caatgaaaaa gtcactgttc caaagttccc gcatcaggtc gtcacgtgct acgatgttgc
     7021 ctgcatgctc aaataacacg cgtaaaatct ggaattcatt cttggtcaaa ttcaagactt
     7081 gcccttgata atgtaaatcc atggatttgg tattgaggat aacaccagca tattccagca
     7141 aactctcatc acgcccaaac tcataggaac gacgcaacaa gccctgaacc ttagctaaaa
     7201 gaacctgctg gtcaaaaggc ttggtcacaa agtcatccgc ccccatattg attgccatga
     7261 caatatccat agcctggtct ctcgaagaaa gaaacatgat aggtaccttg gaaatcttgc
     7321 ggatttcctg acaccagtga taaccattaa acaagggcaa accaatatcc atgaggacca
     7381 gatgaggttc cgactgaaca aatagactca aaacttccat aaagtcttct accaggacca
     7441 cttcaaatcc ccattcagag agcattttcc caatctgttg acgaatgacc tgatcatctt
     7501 ctattaataa aatcttgtgc atgcgcttct ccttttccat tattataaca gatttttcca
     7561 tgctagatgg tctgaaactg aatttgaaat agcctgtttt tagccagtgc aaacaggcta
     7621 tgctactagc taatttgagg gaaatttgct aagataaata aaaagaaagg agctcttatg
     7681 gccaatattt ttgactatct gaaagatgtc gcatatgatt cttattacga ccttcccttg
     7741 aatgagttag acattctaac cttaatagaa atcacctacc tctcctttga taatctggtc
     7801 tccacacttc ctcaacgtct tttagatcta gcacctcagg ttccaagaga gcccaccatg
     7861 cttactagca aaaatcgcct tcaattatta gataaattgg ctcaacacaa gcgcttcaaa
     7921 aattgcaaac tctcccattt tatcaacgac atcgaccctg aactgcaaaa gcaatttgcg
     7981 gctatgactt atcgtgtcag cctcgatacc tatctgattg tctttcgtgg gacagatgac
     8041 agtatcattg gctggaagga agatttccac ctgacctata tgaaggaaat tcctgctcaa
     8101 aagcacgccc ttcgctattt aaagaacttt tttgcccatc atcctaagca aaaggttatt
     8161 ctagctgggc attccaaggg aggaaatctc gctatctatg ctgctagcca aattgagcaa
     8221 agtttgcaaa atcagatcac agcagtttat acatttgatg cacctggtct ccatcaagaa
     8281 ttgacacaga ctgcgggtta tcaaaggata atggatagaa gcaagatatt cattccacaa
     8341 ggttccatta tcggtatgat gctggaaatt cctgctcaca aaatcatcgt tcagagtact
     8401 gccctgggtg gcatcgccca gcacgatacc tttagttggc agattgagga caagcacttc
     8461 gtccaactgg ataagaccaa cagtgatagc cagcaagtag acacaacctt taaagaatgg
     8521 gtggccacag tccctgacga agaacttcag ctctacttcg acctcttctt tggcactatt
     8581 cttgaggctg gtattagctc tatcaatgac ttggcttcct taaaggcgct tgaatacatt
     8641 catcatctct ttgtccaagc tcaatccctc actccagaag aaagagaaac cttgggtcgc
     8701 cttacccagt tattgattga tactcgttac caggcatgga aaaatagata atactcttga
     8761 aaattaaatg tatacaaaac aaaagaccta gaatacatac tttcatgtgc attctaagtc
     8821 tttttaaata gaatctaata gtcaataaaa atcaaagagc attgagagat aatggggctt
     8881 ggaacgtccc tctcgcttca acaaaatgac cccattatag attaaaaaga tgccacttag
     8941 aaaaagcaaa aaaggaagta agacaaaggc aaatacataa aaagctaact gaacattctc
     9001 gtatccattt ttataaaaaa ggtaggatag ataaaaataa cttgaaatga gggataataa
     9061 aaataatact ggattccaca aacttctatt atccttccaa aatgacacta taaaggctaa
     9121 tacaattcct ataacgagat acatttctta ctcctttaat agctacattt tatcataatt
     9181 atccaaagaa aaaagagggc atttatccct cttaatcctt catctgactc tctgcatcgg
     9241 ccacgacttt ttctagactg gtttgaccaa gttctgcctc catagtcaac tgaattctct
     9301 ccaatttttg atccaaaaca tcatgaatat gagctcctac agggcaattt ggattcggat
     9361 tgtcatggaa actgaagagt tgacctgtct taccaagaca ttcgaccgcc tgataaacat
     9421 ctaaaagact aatatcctta aggtccttga caatatctgt tccgcccgtt ccacgcgcta
     9481 ctgaagtcag ctctgccttc ttcaactggg acaagatctt tctgataatg acaggattga
     9541 ccccgacact agcagccaga aaatcactgg tcaccttgct tttcttcccc tcgagggcaa
     9601 tgattatcag catatgagtc gcaatggtaa atctacttgg aatttgcatc ctcttctcct
     9661 ttttacgagg ctaccctgcc tctactcttc tttttctatt attataccct ttttagttgt
     9721 aatgtcaatc gttaccactt ttcaaccagt cgtctaactc ccgatcgcaa ccctctttct
     9781 gagccaattc tctcaaaaat tcctgatgat gagtatggtg gatcccattg accagacttt
     9841 catagtaaac ctcaaaatag ggaagtctca ggtctttagc cagctgcaat tcagctgcca
     9901 catcgtaatc tacccgtcgg aagtccatat ctaccaggcc cttgtcatca aactccaaaa
     9961 tcatatactg ggcccgcaag tccttccgta gctgagcgtc caaaaagaaa ggttggccaa
    10021 tcgaacccgg attgacaatc aattgcccac cagtcccgta acgaagcaac tgctggtgaa
    10081 tgtgtccata aacagcaata tcacagggag gatgagtcac caagcggtca aattcctctt
    10141 gtttgccagt atgaatcaac tctcgccccc agttcttatc aggcagatga tggctaattc
    10201 ccactgtcaa atccccaaac tgacgatgaa tttgaagagg ttgattgtgg agcacttcaa
    10261 tttcttctag ggaaatttcc tctaaaacat actggcactg gcgcaagaga tagcgttgac
    10321 tagggcgagt actatccaat tccttacgga caccatgcca aagactgtct tcccagtttc
    10381 ccaaaactct agccgtaatc ggtagttgat ccaacaagtc caaaatcctt ctacgccctg
    10441 tccctggcat gagaatatct cccaaaagcc agtattcatc cactcctagc tgccgagcat
    10501 ctgccaaaac agcctccaag gcggtggtat ttccatgaat atctgaaaga agagctattt
    10561 tcgtcatatc catctcctcg ttttttctct tgcaataagt ataacataaa aagtcacagc
    10621 tagagaaatc tagctttttt tgatatacta gataaagata ttagacaaga ggaaacgaat
    10681 gaccccaaac aaagaagact atctaaaatg tatttatgaa attggcatag acctgcataa
    10741 gattaccaac aaggaaattg cggctcgcat gcaagtctct ccccctgccg taactgaaat
    10801 gatcaaacga atgaaaagtg aaaatctcat cctaaaggac aaggaatgtg gctatctact
    10861 gactgacctc ggtctcaaac tggtctctga gctctatcgt aagcaccgct tgattgaagt
    10921 ttttctagtt catcatttag actatacaag tgaccagatt cacgaggaag ctgaggtctt
    10981 ggaacacact gtctctgacc tgttcgtgga aagactagat aaactgctag gtttccctaa
    11041 aacctgcccc cacggaggaa ctattcctgc caagggagaa ctcctcgttg aaatcaataa
    11101 cctcccacta gctgatatca aggaagctgg cgcctaccgc ctgactcgga tgcacgatag
    11161 ttttgacatt ctccattatc tggacaagca ctcacttcac atcggtgacc agctccaagt
    11221 taagcagttt gatggcttca gcaatacctt cactatcctc agtaacgacg aggatttaca
    11281 agtgaatatg gacattgcaa aacaactcta tgtcgagaaa atcaactaat ttctcaagtc
    11341 ccctaccaac cctgaaagtt ttattttcag ggttttattt ctatcatttt attttacttt
    11401 catttggtgg tagaatattt gaaaaaaaat gggagaatat ttgaaaaaaa gaaaggtagt
    11461 ttaacctatg aaaaaatcac taaaaatttt tgctacatct aaatagtttg acctcttcgg
    11521 ggttgctttg gtcgttggat tgcgattgca tctggttacc tcaactcacg cctcgataaa
    11581 tttgtagact ggggaccatg gacagctctt gtaccctttg gattgatttc cgtaaccaac
    11641 gttgggattt ccatgttgtc cactcgtttc acggggaaat taagcaaatg ggaaaattac
    11701 tttggtattg ttaataccat tttgtccggt gctattgatt atatccttgg aaataaggcg
    11761 gccatcatta cctatcccgt caccttcctc atttatacct ttgctataaa gaaatggaaa
    11821 gcttcgcaag aaggcagacc caaccaaatg agccaaaaac aggtaaaatt ggcggccatc
    11881 atcatttcca tcatcgcctt cctctttgcc tttgtgacca actatatcgg atatgggggc
    11941 aagatgaatc tccttgccta cgtaacaact attgcctttg cactgtccct cattgccaat
    12001 gctttgaatg cattggcaat gagggacagt ggggcttttg gttgatttac aatttcgttc
    12061 agctgacaaa agctggtatt caaggaaact tcgccaatat cggtaaatac atcttttata
    12121 tcctcaatgc aatcggagct ttatttgtct ggaatgatga agaagttaga taatagaagg
    12181 aaactcaaat aaagaatact agccaactta agagaatctc ccctctgcta tttttatcat
    12241 tttagttgta actcccattc ttttagagta gaatgaagtt agataaaaga gggaggtctt
    12301 gtcatgaaaa aactctatag aatccatttt atagcaattg cagttattga tttgctacta
    12361 tttgcatttt ttatcactag actcgagaca tcttttgagt ggctcttgct ctctggttta
    12421 attttcttcc ttgctcaagg actcctgcta tttctcttgg tcgtccgact caaacatcaa
    12481 ttcgctgaga tttatcctca aatcaataaa aagattcgct tctactattt aggggttctc
    12541 accattgatt ttctattttt tgttctctta gccttcatta gttctcagcg tttttcatct
    12601 cttatgccaa tcatcactgc ttgccattct actttttatt atatgacagc tgactaccta
    12661 agagaaaact atccagactt ttacgacaaa cacatctcta acacgtatac tattttatcg
    12721 atttcttctt ttttagtata agacagctcg ttctccataa atattcatta actttcattt
    12781 ttatactttt cctttcgatt ttcaaaaaca aggggaggga ttaacctccc ttatattaga
    12841 cataactttt aggatcacaa ttgtatgttc ctttcgaaat atccccaaaa atcgtagccc
    12901 ctgttaaata atgatttctt gtagaagcat atgctatttt cttcatcaca tccagtctcg
    12961 ctacttttag ctcgagatta ggattggaaa acatagcatt tttaatatct tccaaatctt
    13021 gagaactagg tgcgagtaga gttacatcgc cctccaatct attagccttg tctatataaa
    13081 gactaactct ttcagcactt tctgttcgca gaaaatttag tacatttcta ttcatttttt
    13141 tacctcgtat ttttctaatt attgattttt tctagaaaca aaatcaattc aacggtgaag
    13201 ctctagcttt catcttcatt cgtacctcct tttgtgtata tctagcttta cataaaccta
    13261 tgatttagag tatagtttct caattattct cagaaactat ggagatgatt taattatcat
    13321 acaaatcaaa gacctctcct tgctcatctt caccgtaact ctcgagttcc tcataaaagt
    13381 attccaaagc agtttgttct tctggtgtca taaagaaact cctttctaaa caatattagt
    13441 aaaaaatacg ttcttatttc catcctaaat aaagtagtgt ttttatcccg taataggttt
    13501 tatttttgcg gagcattaca ctccgcatta atattgatag tattaagaat cattattact
    13561 taataaaaat tgcgctctca aacatagacg tatacaaaat gccctatgta tacgtttcgc
    13621 ttggaagaca tctaagaaaa aattttgtag ttcagacagt aagattaata catctttctc
    13681 taacataagt gatgtctggt actcccaatt attgctctgt tttaacgatg aaaaaggtat
    13741 agctgtctct tttaatttta tccaatctat acgttctttt ttaggaacgt attctctaac
    13801 agcttgacta acaatactgg aatagcttac ttcatagcct aatttattgt aaacatcatc
    13861 tttaagctta tctaatatct gtctggattc agacgctaaa cgcattctaa cattctcttt
    13921 tcccatatta tcaccccttt ctgacgggat tctgttatcc cgtaaattaa gtatataaca
    13981 atttagaaaa aaaatcaaga attaagttga aaaaaattcc acaatctaaa aatttatcat
    14041 tttatgacaa acatactatc gttaaatgct gatttaatcg aaattttgag ttataaactc
    14101 ctcagtttcg aactcaaaat tagccgtgca ggataggata cccttcataa acttatataa
    14161 ctactttaat accttaaaaa gctaataaaa taaggaatat ctaagcaata tacattctaa
    14221 aatacctgta cgggagtgtc tctaaagaaa aggaggtttt agcatgaaaa aaatcatctt
    14281 catcaaaacc attcaactcc ttgtcattga tggaatcatg ctggcatttt tgacatttaa
    14341 aaggggggct tacttgggac tggattttga tttatagtgg ttggctcatt ttctttcatc
    14401 ctgtgctatt gacctatctt tcaaaccaac tttgtgacca ctttagtcaa ctctattccc
    14461 agattagacc gagattctgg cgttttgctt tacaaattct cctatgggat agcctgatga
    14521 ttctctcctt ggtgtcttta agtgatattc cacttttcct tcagggaact ctcctcatcc
    14581 taggacatct catcccttcc tatcgcatct gccaaagcct gaaaagagac ttcccccaag
    14641 catatcaaga accgatttct ttttggagta ttttatgatc gatgagaaag accaagccga
    14701 ctgggcttgg tctttcttat ctctttttag tatctaggat aatggtaaca ggtccgttat
    14761 taaccagctc aacctgcata tctgctccaa agatacctgt ctgaacgggc acttcttgcg
    14821 ctaatttttg attgaaagca tcatagaagt ctgatgccat atcaggttta gctgcccctg
    14881 taaaggctgg acgattgcct ttcttagtat ccgcaaagag ggtaaactga gaaatagaga
    14941 ggatttctcc ttcaatatct ttgacagaca ggttcatctt gccttctgcg tctgaaaaaa
    15001 tccgcatatt gaccagtttt ctcacagcat agtccaaatc ttcctcttgg tcctctggtc
    15061 caacaccaac cagcaataaa agtccctgat tgatttttcc ctgaatctgg ccttctatac
    15121 tcacttgggc ttttttaacc cgttggataa tgattttcat aatagccttt ctagtaagag
    15181 ctaggacaac tagccgttgg tccgtttgac agagtaaact tctggcacac tcttaatttt
    15241 atcgacaacc gtggtcagtg tagagaggtt ggcaataccg aaggacacat ggatattagc
    15301 aaacttcata tccttggttg gttgggcatt gaccgttgaa atattcttgg ttgtatttga
    15361 aagaacttgc agtacatcgt tcaacagtcc tgtacggttg agaccgtaga tatcgatatg
    15421 ggccatatac tccttatttg agctagagta ctggtcttcc cattccacat caaggagacg
    15481 ttgctcgtag ttttcttggg cacgcaggtt catacagtcc acacggtgaa tagccacacc
    15541 acgacccttg gtaatgtagc caacaatatc gtcaccaggc acggggttac aacacttagc
    15601 aatccgcact aggagaccag aagcaccttc aataaccact cccccctcat gcttgacctt
    15661 gagagtttct ttattttcaa ccttgacctc gccacctttg acaagctcct ctgcctcagc
    15721 cttggccttg gcacgctctt cctcacggcg ttccttttca gtcagacggt taaagacggt
    15781 aatcgcaccg atttccccaa aaccaatggc cgcaaagagg gagtcttctg tcttgtaact
    15841 ggtcttttgc agaacttgat ccatgtggcg cttgtccata aatttatttg ccacatagcc
    15901 attttcttgg aactgagcca tcagcatctc acgacccttg ttgacagaca attccttatc
    15961 ttggttttta aagaactggc gaatcttatt gcgcgccttg ctagtcttga ccatattgag
    16021 ccagtcacgg ctaggtccaa aggagttcgg gttggcgata atttcaacct gatcccctgt
    16081 ctttaacttg gttgtcagtg gaaccatgcg gccattgacc ttggcaccag ttgctttttc
    16141 accgaccttg gtatggattt cgtaggcaaa atcaatcggt cctgaatctt tgggaagaga
    16201 acggacagct ccatctgggg taaaaacgta aatctcctca gccagatagt tttccttaac
    16261 agagtccaca aattccttag catcatcagc ctggtcttgg agctccatca tctccttgat
    16321 ccagttcatt ccaatagctg attccttgct gttaacttgc ccctttatac ctttcttata
    16381 agcccagtga gccgcaaccc cgtactcagc cacctcgtgc atttccttgg ttcgaatctg
    16441 gaattcaatc ggcccttttg gtccataaac agtcgtatgg atagactgat aaccattagc
    16501 cttgcggttg gcaatatagt ctttgaagcg acctggcatc ggtttccaaa attcatgcac
    16561 gtaaccaagc atggcataaa catcactttg ggtatctaaa atacaacgaa tagcaatcag
    16621 atcatagatt tcctcaaacc gttttctctt gtcctgcatt ttgcggaaaa ttgagtaaat
    16681 atgcttggga cgaccataaa tcttcccttt caagtgacgt tctgtcgtat actcctctaa
    16741 ttttgtgact acctcatcca ccaaggcctc acgctccctg cgcttttcct tcatcatatg
    16801 ggtaatcttg taaaactccg ttggattgag ataacggaaa gacaagtctt ctaattccca
    16861 tttgacactg gaaatcccca aacgatgggc aagcggggca tagatttcca tggtttcttt
    16921 ggaaatacgc tcctgcttgt cttttcgaag atgtttcagg gtccgcatat tgtgcaagcg
    16981 gtcagacagt ttgaccaaaa taacgcggat gtcctcagac atggccatga gcatcttgcg
    17041 atgattttcc gctaattgct cctcgatcga tttgtactcg accttgccaa gcttggtaac
    17101 tccgtcaaca atcatccgca catcaggacc aaactctctt tccaaatcgt ccaaagtcgc
    17161 atctgtatct tccaccacat catgcaagaa tccacaagct actgttacag catccagctt
    17221 tagcttagct aaaatacctg ccacttggat agggtgaatg atataaggct cgcctgattt
    17281 gcgatattga ccactgtggc attcaacagc atagaccaag gccttatgga caaaatgaac
    17341 atcctcttcc gttaaatatt ctttggttaa agcgacaact tcttcgcctg ttaaattcac
    17401 ttctttcggc atctctactc tccaattctt cctaccattt tatcactttt ttaagaatat
    17461 gaaaactaga ttggaacaga ataagaaaaa aataattcaa aattgcttga taattctgaa
    17521 ttattggccc ataatatact acgaagttag attttaaact taggtgatag aaggagagat
    17581 agaagaacgg aaaccatatt gtaacccaaa gactttctga tttccccaat tccattgaag
    17641 atacgaaaga taaacggtgg aactcgtatc acatacactg gtaccttgac tggattttgg
    17701 aattaatact caatgaaaat caaagagcaa actaggaaac tagccacagg ttgctcaaag
    17761 cactgctttg aggttgtaga taagactgac aaagtcagtc acatat
//
LOCUS       DAVBCW010000034        17332 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVBCW010000034 DAVBCW010000000
VERSION     DAVBCW010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17332
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.034"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(<1..692)
                     /locus_tag="VQ514_000983"
     CDS             complement(<1..692)
                     /locus_tag="VQ514_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HET2499755.1"
                     /translation="MNIILIAKPLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSYRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDG"
     gene            complement(803..1057)
                     /locus_tag="VQ514_000984"
     CDS             complement(803..1057)
                     /locus_tag="VQ514_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499756.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRITNRLANDLNNVLKQV
                     DKDTPNTPTWLNSAASKAKDDDRVYKLLKTLIPGENYLSC"
     gene            complement(1415..1948)
                     /locus_tag="VQ514_000985"
     CDS             complement(1415..1948)
                     /locus_tag="VQ514_000985"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HET2499757.1"
                     /translation="MTSFKEQEPEKVAEFLDILDNLKDLPVLYIDETGIDRYLYRPYA
                     GAPRGEKVYEKISGRRFERTSIVAGQVDGEFIAPMIYKKSMTSDFFVEWFKTQLLPAL
                     KTPHVIVMGNASFHPKNILDELCIQDKHFFLPLPPYSPDLNPIEQAWAILKKKVMDLL
                     REVPTIFECLECFFKTR"
     gene            complement(1935..2150)
                     /locus_tag="VQ514_000986"
     CDS             complement(1935..2150)
                     /locus_tag="VQ514_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2499758.1"
                     /translation="MGKERREQGHIERKKRVVKNRKIPLEELKAFVEAHPDAFLREIA
                     AHFDCAVPSVWAALKQIKVTLKKDDEL"
     gene            complement(2117..2260)
                     /locus_tag="VQ514_000987"
     CDS             complement(2117..2260)
                     /locus_tag="VQ514_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tnpB"
                     /protein_id="HET2499759.1"
                     /translation="MAYSTDFKQRALDYIKEGHSHVEAAKFFGVGVRTLFTWEKKDVN
                     KDT"
     gene            2494..4212
                     /locus_tag="VQ514_000988"
     CDS             2494..4212
                     /locus_tag="VQ514_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HET2499760.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDVEYLKEVKDVNI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKIANIEAE
                     INAFVK"
     gene            4323..4670
                     /locus_tag="VQ514_000989"
     CDS             4323..4670
                     /locus_tag="VQ514_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET2499761.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFIHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(4949..5785)
                     /locus_tag="VQ514_000990"
     CDS             complement(4949..5785)
                     /locus_tag="VQ514_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2499762.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPELLKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(5798..6427)
                     /locus_tag="VQ514_000991"
     CDS             complement(5798..6427)
                     /locus_tag="VQ514_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2499763.1"
                     /translation="MLELRNINKVFGDKQILSNFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLDNLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(6437..7078)
                     /locus_tag="VQ514_000992"
     CDS             complement(6437..7078)
                     /locus_tag="VQ514_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2499764.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(7224..8453)
                     /locus_tag="VQ514_000993"
     CDS             complement(7224..8453)
                     /locus_tag="VQ514_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET2499765.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLAL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(8443..9609)
                     /locus_tag="VQ514_000994"
     CDS             complement(8443..9609)
                     /locus_tag="VQ514_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET2499766.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            9753..10766
                     /locus_tag="VQ514_000995"
     CDS             9753..10766
                     /locus_tag="VQ514_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HET2499767.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FVRMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            complement(10811..11230)
                     /locus_tag="VQ514_000996"
     CDS             complement(10811..11230)
                     /locus_tag="VQ514_000996"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HET2499768.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(11241..12647)
                     /gene="atpD"
                     /locus_tag="VQ514_000997"
     CDS             complement(11241..12647)
                     /gene="atpD"
                     /locus_tag="VQ514_000997"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET2499769.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMEF"
     gene            complement(12733..13611)
                     /locus_tag="VQ514_000998"
     CDS             complement(12733..13611)
                     /locus_tag="VQ514_000998"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HET2499770.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            complement(13627..15132)
                     /locus_tag="VQ514_000999"
     CDS             complement(13627..15132)
                     /locus_tag="VQ514_000999"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HET2499771.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQSVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            complement(15147..15683)
                     /locus_tag="VQ514_001000"
     CDS             complement(15147..15683)
                     /locus_tag="VQ514_001000"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HET2499772.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            complement(15683..16177)
                     /gene="atpF"
                     /locus_tag="VQ514_001001"
     CDS             complement(15683..16177)
                     /gene="atpF"
                     /locus_tag="VQ514_001001"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HET2499773.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            complement(16191..16907)
                     /gene="atpB"
                     /locus_tag="VQ514_001002"
     CDS             complement(16191..16907)
                     /gene="atpB"
                     /locus_tag="VQ514_001002"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HET2499774.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            complement(16942..17142)
                     /locus_tag="VQ514_001003"
     CDS             complement(16942..17142)
                     /locus_tag="VQ514_001003"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HET2499775.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            complement(17217..>17332)
                     /locus_tag="VQ514_001004"
     CDS             complement(17217..>17332)
                     /locus_tag="VQ514_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HET2499776.1"
                     /translation="NYPKKCLDYKSPREDFWMANLNLKFSKMEIIFIKRFQ"
BASE COUNT         5229 a         3795 c         3112 g         5196 t
ORIGIN      
        1 ccgtcagtag tagtcatgct actgagtttt ttatctgtga cattgtagct ggtaccaatc
       61 agttgttttt tattggtaat gtaggttccg tcactttctt tttctccaat tccagtatca
      121 tttttcaatg atagcaaacg cccttgttca tcaacatagc gaactttcac attttctgag
      181 attagttctg ccaattctga ggtttttttc tttttcttga tttcttcggt tattttccct
      241 ttctcttctt cgggaatatt tagttttgga atgatttttt caacaacggt tcgtgatggt
      301 tccacagtat cttggatgac tgaaaagtca gctagaattg ggagattata ctgaacacgg
      361 taactttgag tgtttactcc tactctttca ttattctctg aaaatacttg tacggtataa
      421 gaaacaacat cttttcctaa tagaacatcc ccagtagaga aatagccgcc ttttcctagt
      481 ttgctatctc cagagcccac ttctttccta attttatcag atagtttttt accagtcagt
      541 acattcgttc gcacaatccc tttgtctacc cctacaaagt gggagaactt tttgaactct
      601 tcagaaccag atctagccca accattatta agggcatttg cttttgtatt tgtattctct
      661 ctcaaaggtt tggcgattag aattatattc atcggcactt agagttgctg ctatatctga
      721 ctcttgaata ccaacttcct tactaccatt tctagcggca gtatatgtgt atttatctgt
      781 ttctacttct agctgattat ccttaacatg ataggtaatt ttctcctggt ataagagtct
      841 tcagtagttt atatactctg tcatcatctt tagctttaga agcagctgag tttagccaag
      901 ttggggtatt aggagtatct ttatcaacct gtttcaagac attattcaag tcatttgcta
      961 gcctattggt tatcctcact gtgaaagttt tcttccagtc tgaaccactt ttattagact
     1021 tagatatgct catgaaatcc tggtcaacat tcaacacaat atcgccttgt tttttagcat
     1081 cttccgttgc ttctgcttca aattgatcat ggagtttttg ttgctctggt gtatagtttt
     1141 tttatttaca cgatttaatg tactattttt tattttagta ttaagaactc cattaatacg
     1201 atttttaaac tctgcttcaa aaaatccctt aatttgttgg tgcgcaagtt caacattcaa
     1261 acctttctta tacaagtctt cattctgata caagtcttca ttctgaacga tttcggctac
     1321 acggtgaatg gtttgatcgt ataccgtctt ctcttaacca gcagtatcct tagttttaat
     1381 gactactcga taggttcctt tagaaccgtt atagtcatct agttttaaaa aagcattcca
     1441 aacattcaaa aatagttgga acttccctta ataaatccat cactttcttt ttcaagatag
     1501 cccaagcttg ctcaatagga ttcaaatccg gtgaataagg tggtagaggt aagaaaaagt
     1561 gtttatcttg gatgcagagt tcatccaaaa tgttcttggg atgaaaacta gcattgccca
     1621 tgacaataac atgaggtgtc ttcaaagcag gtaggagttg cgttttgaac cactccacaa
     1681 agaaatcgct tgtcatgctt ttcttgtaaa tcatgggagc tataaactct ccgtctactt
     1741 gtcctgcaac aattgaagtt cgctcaaaac gacgtccgct aatcttttca tagactttct
     1801 cccctctagg agcccctgca taaggacgat agaggtagcg gtcgattcct gtttcatcaa
     1861 tatataggac tggtaaatct tttaggttat ccaaaatatc aagaaactca gcgactttct
     1921 ctggctcttg ttccttaaag ctcgtcatct ttttttaaag tgaccttaat ctgctttaaa
     1981 gctgcccata ctgaaggaac agcacaatca aaatgtgccg caatttcccg taaaaaagca
     2041 tctggatgag cctctacaaa ggctttcaat tcctctaaag gaatctttcg gtttttgacg
     2101 actcgctttt tcctctctat gtgtccttgt tcacgtcttt cttttcccac gtgaagagag
     2161 ttctgacgcc aacaccaaaa aactttgctg cctcgacatg gctgtgcccc tctttgatgt
     2221 aatctaatgc tcgctgttta aaatcagtac tatatgccat agctttatta taatatgctt
     2281 gtttaaaact aagcaactat atcaacgttt tgttatgaga agacttactt tttttactga
     2341 aattgaattt ttcctagcct caacatcata taaccccaaa attcaatctg acctggtttg
     2401 aagagatttt cgaagcagat aaggttttac taaaactaaa aaaaatgata taatcaaact
     2461 gattaaatgt tttaaaacta aaggagaatt tgaatgtctt accaagaaaa ttaccagaaa
     2521 tgggttgatt ttgtagagct tcctgactac cttcgtcaag atttggaaaa tatggacgaa
     2581 aaaactaagg aagatgcctt ctatacaaat cttgaatttg gtactgcagg tatgcgtggc
     2641 ttggttggtg ctggtacaaa ccgtatcaac atctacgttg ttcgccaagc tactgaagga
     2701 ttggctcgtt tgattgagtc aaaaggtgga aacgagaaag aacgcggtgt agcaattgcc
     2761 tacgatagcc gtcacttctc acctgagttt gcctttgaat ctgcggcagt tcttgctaaa
     2821 cacggcatca aatcttacgt atttgaaagc cttcgtccaa ctccagaact atcatttgca
     2881 gttcgtcatc tcaactgttt cgcaggtatc atggtcacag ccagccacaa ccctgctcca
     2941 tttaacggtt acaaggttta cggtgaagac ggtggacaaa tgcctccaca cgatgcggac
     3001 gctttgacta cttatatccg tgcaatcgaa aacccatttg cagttgaagt tgctgatgtg
     3061 gaaactgaaa aagcttctgg cttgattgaa gttatcggcg aagctgttga cgtagaatac
     3121 cttaaagagg ttaaggacgt aaacatcaac ccagccttga ttgaagaatt tggtaaagac
     3181 atgaagattg tctacacacc acttcatggt actggtgaaa tgttggctcg tcgtgctctt
     3241 gcccaagcag gatttgactc tgttcaagtt gttgaagcgc aagcaactgc tgacccagac
     3301 ttctcaactg taacatctcc aaacccagaa agccaagcag cctttgccct tgctgaagaa
     3361 cttggtcgtc aagttggtgc agatgttctt gtcgcaactg acccagacgc tgaccgtgtt
     3421 ggtgttgaag ttcttcaaaa agatggtagc tacctcaacc tttcaggtaa ccaaatcggt
     3481 gctatcatgg ctaaatacat cttggaagct cacaaaaacg ctggaactct tcctgaaaat
     3541 gccgctctct gcaaatctat cgtttcaact gacttggtaa cgaagattgc tgaaagctac
     3601 ggcgcaacta tgttcaacgt cttgacaggt ttcaaattta tcgctgaaaa aatccaagaa
     3661 ttcgaagaaa aacacaacca cacttacatg atgggatttg aagaaagctt cggttacttg
     3721 attaaaccat ttgtacgtga taaagatgcc atccaagccg ttcttgtcgt tgctgaactt
     3781 gctgcctact accgttctcg tggtttgaca cttgctgacg gtatcgaaga aatctataaa
     3841 gaatatggct actacgcaga aaaaacaatc tctgttactc tttcaggtgt cgatggtgct
     3901 gaacaaatca aagcgattat ggctaaattc cgcaacaatg ctccaacaga atggaacgca
     3961 acagctatca ctgtcgtaga agacttcaag gcacaaactg ctactgttgc tgacggtact
     4021 gttacaaact tgacaactcc tccaagtgat gtgttgaaat acacacttgc tgacggttca
     4081 tggattgccg ttcgcccttc aggtacagaa ccaaaaatca agttctacat tgcagttgta
     4141 ggggaaacca acgaagaatc acaagctaag attgctaaca tcgaagcaga aatcaatgca
     4201 tttgtaaaat aaagacctat aaaagatgag accaaccaac ctcatctttt ttcgtatcaa
     4261 atctaagcaa ttttagaaac tttatggcat actagactta tcaatgaaag cgaggtaatc
     4321 tcatggaaca atttttagat aacatcaaag accttgaagt cactacagtt gtgcgtgcgc
     4381 aagaagctct tgataaaaaa gaaactgcaa ccttctttat cggtcgcaaa acttgccctt
     4441 actgccgtaa atttgcaggt acattgtcag gtgtcgtagc tgaaaccaaa gctcacattt
     4501 acttcatcaa tagtgaagaa ccaagccaac tcaatgattt gcaagcattc cgctcacgtt
     4561 atggaatccc aactgtacca ggttttattc atattacaga tggacaaatc aacgtccgtt
     4621 gcgactcttc aatgtcagca caagaaatca aagatttcgc aggattgtaa aaaatcagct
     4681 aacaagtctt aaaatcacaa aaacgcataa tgtaaggtgc aaaaaccttg atattatgcg
     4741 ttttattgtt cctaaaacag ttacatcagc atttcattac agaacatttt aattttggaa
     4801 gtcaaatcaa tttttaacaa tgttttagaa atcacagtgg gctattccat attcaattat
     4861 ccagattcaa tcgctattat cgttttccta gattacaaaa aaaactacgt tttgctaatc
     4921 aaaacgtagt ctcagccact attttatctt actgtccttc ttttacttct ttggttgcta
     4981 catcttctcc aaaccatttt tggctgattt cttggaactt gccgtccttg taaagactag
     5041 aaaaagcttc atttatcttc ttaaccaagg ttgtatcttc cttacgggct ccaaccgcaa
     5101 aagcttctgt ttctagtcca actgtaaaga cattataatc gtttaaaaca ccttctgctt
     5161 ctaaataata gtttgcatag acacggtcaa tcaatagacc atcaattcga tcgtttttca
     5221 aatcaatcaa ggcttcatta aaggtttggt attgattcgc ttccttatta gcgacaatat
     5281 tcttcaaaag ttctggattt gcttcaaagt ccgcataacc agatgaacca gcttgagctc
     5341 ctaatgtctt tccagtcata tcctttgcag tcgtgatacc agatgatttc ttcgtaacca
     5401 atacctgctc attcttcata tatgagttac tgaaagccac cttttcacgg cgttcgtctg
     5461 tagcggaata gccattccaa atcagatcaa tcgttccttt tgtcaattca gcttctttca
     5521 aatcccaatc aatcggttgc caatttaccg tgattccgta tttttcaaaa acagctgtag
     5581 ctaaatcaat atcaaatcct gcataagaac catctttctg agcaaatccc attggaacaa
     5641 aagtactatc aaatccaata gtaatagact tgttagactg gtactttgac caattatctc
     5701 cactagtttc gctagaattt ttcccacaag ctactaagaa caaagcagtc atcagactga
     5761 ctaatacaag catccatttt ttcatccatt tttcctccta tttaggttct actttcaata
     5821 atacatctgc gatattttca gcaaactgca aatcatgggt aaccacaatc tgggtcatcc
     5881 caagttccct attttgcaag attagcttct ccacttccaa acgtaattct ggatccaggg
     5941 cagaagttgg ttcatcgtag ccaatgattt ctgggtcaat catcatagca cgcgccaaag
     6001 ccacccgctg cttttgccca ccagatagtg agaaaggata ggcctctgcg tgtcctccta
     6061 gtcctaactg ttccaagagt ccactcgcct tcttctcagc ctcttcctgc ttcattccca
     6121 tggtcttcac aggcgataag gtcaaattgt ccagaactga taaatgaggg aacagttgaa
     6181 aatcttggaa gacaaatccc agtagattgc gcttctgcaa ttcatccagc tctaaaggtt
     6241 gtccattata aaagatttgc cctgaatcaa tggtttcaag acctgcaagc atacgtaaaa
     6301 gagttgtctt acctccacca gaaggtccaa cgatagccag gatttgcttt tcaggaatac
     6361 ttagactgaa attagacagg atttgtttgt ctccaaagac tttattgata tttcgtaatt
     6421 ctaacatggc agcctcctat ctataataac tatacttctt ctcaactttt ttggaaataa
     6481 ttgtcacaat cccaatcaaa atcaaataaa tggctcctgc caagaacata ggaactagac
     6541 tagcatcgcg gttagcagct gttcgactag ccaagataag atctgaaatt ccgagagcat
     6601 agaccaaaga agtatccttg accaaactca taacttcatt aaagacacta ggaagaacga
     6661 tcttggtcac ttggggcaag ataatatagc gcactctgtc aaaagggcta aacttcaaga
     6721 ccttggcggc ctcatactgt cctcttggaa tagtgtcaat tcccccacgg aaaatttctg
     6781 caaagtaagc tgcatagttg agaacaaagg caataatagc tgcaggaagg cggtctaaac
     6841 gaatcccaat acttgggagc acataataga taaaaatcag ttgcaagagt aagggggttc
     6901 ctcgcataac ccagatgtaa atgttaatca gataatggag gggcttccaa tggacttgca
     6961 aggcaaaggc aatcaaaacg cccaacggaa tcgaaaatag caagaccagt gcaaagacct
     7021 gtacagtcgt gctcgcacca ttcagtaaac tcggtaatat ctcaaataaa taagacatac
     7081 tgcacctcct aaaagtaatt gttcctatta tagcatgaat aaacaagatt accaagtatt
     7141 tttgtaaaaa gattctacca tatacaaata aaaaccgtag ctatcaaaag agataaacta
     7201 cagttctata tgtttaaaca ttcttacaat ctctcaaaca gttcttgcat ttgcacatca
     7261 tctggaacca gttttaagta agtgtgagca tggacttttg cttcttcaaa atgtcccaat
     7321 tcacgcaaga gatagatata gtgttccaga aattctggat tgtccttcaa atctcctgtc
     7381 aactcttgat aatgctcata agcagtatcc aaatcgtcca tttcttgata agaacgagca
     7441 atcatccact tggtcaaaag attttctggc tcctcactct gcaagtctag aatatcctca
     7501 taacgctcct gctccagata aatagtggct aaacgaagca agatttcttc tgtatcctca
     7561 gcgtcttctt ttgcagtaag gagataattt tctgcaccac tagcatcatg caattcataa
     7621 gaaaattgtg aagcagctag caagaggcga gtttcaaagg gatttttctc taatccttgc
     7681 ttagcgatac gcagggcttc ttgaacttga tgttccttat gtaaagcctg actgtaccca
     7741 tactcatagc cttcaaagtc aggagaaatg gtatcaagct gcttaaagta gagggtggct
     7801 ttttgatatt cttcttgatc aaaataaaga ctggccaact caaaagctgt taagtcatcg
     7861 tattctaact ccagggcttt ttctaaaaac tcagtagccg tttcaaattt ccctaactga
     7921 gcataggcaa agccaattcg ttgataggtg gaaatgcccg tttgctcata aatcgagcga
     7981 ttatctaact gggcataggc ttgaatagcc gcttggtaat tttccaactc actatccaac
     8041 tctgccaaac ccaatatcaa gagagaatcc tctgagtagg tcaaggcctc caataatttc
     8101 tcacgtgcca catctgtcaa accttccagc tggtaaaggt ctgccttcag agccaaagac
     8161 gagacatacc agtcactgtc agcttggatt tcctcaagat aggtaaaggc ttcttctatt
     8221 tgaccatcct cgctagcaat tgcagctaga ttaagatgaa cctctggaaa atcctctaca
     8281 attttcaggt aaatttcctt ggcctgagga tagaaaccaa tcccttcaag ataagttgcc
     8341 aactcataca gaagatcact tgaatcattt tctaaagctt tggcgaaata atgctcagcc
     8401 ttagttaaat cttgctcctc caaagcctgt aacatctgtt gactattgtt cactcttgac
     8461 ttcctcaccc tctaattcat atagaccact gactacctta taccattcga aaatggctga
     8521 aatgacaacc ttagcagagg cataaaccgg aataccaagt aaaactcccc agataccaaa
     8581 catagatcct gaagttaaca aaacaaagag aacattaata ggatggatgt ttaattgact
     8641 tcccaaaatc aatggagaga caaaacggcc ttcaatagtt tgttctacga taaagacaat
     8701 cactactttc aaaagcatga ctggaccagc aatcaaaccc aatactagag caggaagcat
     8761 ggctagaaag ctaccaagat aagggaccag atttaaaata ccagcagtaa cccccagcgt
     8821 aaccgcatag cgtagaccaa taatcttgaa gaagatgata aacattactg ctacaataat
     8881 agccactgtc acttgccctc gaacatagtt ggacaactgt tgattcacat ctgataaaac
     8941 ttgtccaaca ggttccttca attttcttgg aatgaattgg gtcaaatagt tacgcaagcc
     9001 tttcccatca cgcaagagat aaaagagcat gaaaggaacg ataatcaagg caacaatcac
     9061 ttgagaagcc ccgctaataa aggcactcac ccagttgact gcctgagatg aaaccttact
     9121 tgccaaaact gtagcctggc tagaaaaatt ggtcaaaact tgctctaatt gaggtctgaa
     9181 atcatctggc aggtgctggg ctaccaatcc attaacaatc ctatctatat cttctaagta
     9241 aacaggaacg tttcttgcaa aggtcaaaac ctgacgttgc agatttggaa tggcgactgc
     9301 caagccccaa atgataaaga gagcgatgat aacaaagaca atagtgatag ctataacacg
     9361 attaacctta tgcttctcca tccaatcaac aataggattc aacaaataat ataacaaacc
     9421 agacaaaatg actggcaaca tcacaactgc taaaaagtct aaaacaggtg aaaatagaaa
     9481 actaatctta cttaaaataa aaagattcag tcccaataat aaggttacta aaaataccgt
     9541 aattgccttg ttatctaaaa accacttgaa aaaccaagat aggctaaaat gtttctcttt
     9601 ttgctccata aatacttcct ttctattgtt ctctcattat accatttttg aaacaaaata
     9661 actctttttt aaagtgctta catctaattg gatgtcttta atttgcttgt ttttgtgata
     9721 taatagtctt atctttacac agaggtatag taatgaaaga aactgtttat tttggaactt
     9781 atacacgtcg tacttctcaa gggatttaca aggcagactt tgatacagaa actggtcagc
     9841 tttcaaatct agaacttttt gcagctgagc caagtccaac ctaccttgcc tttgaccagc
     9901 accaacattt atacactgtt ggtagccaag acgataaggg gggaattgca gcctatcaaa
     9961 ctgacgggac tgtgttaaat catgttgttg aagaaggagc tccccactgt tatgttgctg
    10021 tcgatgaaaa gcgtgatttg gtttacgcag ccaactatca caagggacaa gtccttgttt
    10081 ataaacgcca ggaagatggt agtcttctac ttagtgatat ggatcaacac agtggccaag
    10141 gtccacatga aaatcaagct tccccccatg ttcactatac agatttaaca cctgaccact
    10201 atctagtgac ctgcgacttg ggaactgacc aagtcatcac ctatgacctc gatcaagaag
    10261 gaaaattatc taagctctat acctatcaca gcaagccagg agcaggctca cgccatatca
    10321 ttttccataa ccactataaa atcgcttatc tcatttgtga actcaatagt actatcgaag
    10381 ttttaatcta cgatggcgtt ggcgaatttg tacgtatgca ggttatttca actttaccag
    10441 aagcttacga aggctttaat ggaaccgctg ctattcatct ctctaaagac ggtaaatacc
    10501 tctacgcttc taaccgtggc catgattcta tcgcagtata taccatcctt gcggacggta
    10561 gcttagagtt attagaaatc gttccaacgc atggtcagac tccacgtgat tttgatttga
    10621 cccccgacca aaaatttctc attgttgtcc atcaagactc tgacaatgca actgtcttta
    10681 aacgtaattg tgacaatggt cgtctagcag aactctccaa cgacttccat gttcccgaag
    10741 cagtctgcat ccgttttgct ccttaaaaca ccataaaaaa tgaacttggt attttcaagt
    10801 tcattttttc ttatagtcta tttccgacat taatacggtt aatggcacgt tgcaaagcaa
    10861 tcttagcacg acgttcttgg tcaatcaaat gtttgtcttg tgcttcttca attgcacgtt
    10921 ctgcacgaag tttggcacgt tctgcacgac tgatatcgat atcacgagca cgttctgcag
    10981 agtcagcgac gattgtgatc atatcattgg caatttcaat aacgccaccg tttactgcaa
    11041 tccagttcac gtgatcttta tcatcgatac gttttacctt tacttcatca actgctaaaa
    11101 ccgcaatcat attttcatgt cgtggcaaga tccccatctc accatccaga gttcgaaccg
    11161 atacatagct ggcatggtga tcatagacga gaccatctgg tgtcacgatc tggacagtta
    11221 actgagccat aggtcacctc ttaaaattcc attttttcag cttttgcaat cacatcttcg
    11281 atagaaccta caccacggaa ggcatcttct ggcaagtggt cgtatttacc atcaaggatt
    11341 tccttaaagc cacgtacagt ttcagcaact ggaacataag aacctggctg accagtaaat
    11401 tgttccgcaa cgttgaagtt ttgtgacaag aagaactgga tacgacgggc gcgagcaacc
    11461 aaggtctttt cttcatcaga aagttcatcc ataccaagga tagcaatgat atcttgcaat
    11521 tcatggtaac gttgaaggac acgttttact tcagcagcaa ctgcatagtg ctcttctcca
    11581 acgatttcag gtgccaaggc acgtgagctt gaagcaagtg ggtcaacggc tgggtagata
    11641 cccaattgta ccaacttacg ttccaagttt gttgttgaat ccaagtgagc gaaggctgtt
    11701 gctggcgctg ggtcagtata gtcatccgct ggcacataga tagcctggat agaggttaca
    11761 gaacccttct tggttgatgt gatacgttct tgcaattgac ccatttccgt agcaagtgtt
    11821 ggttggtaac caacggctga tggcatacga cccaaaaggg cagatacttc tgaaccagcc
    11881 tgagtgaaac ggaagatatt atcgataaag agaagcacgt cttggccttc cacatcacgg
    11941 aagtattcag cgattgtcaa accagtaagg gcaacacgca tacgtgctcc tggtggctca
    12001 ttcatctgac caaagaccat ggctgttttc tcgataacgc ctgattcttt catttcccag
    12061 taaaggtcat tcccctcacg agtacgttcc ccaacaccag taaatactga aataccaccg
    12121 tgctcttggg caatgttgtg aatcaattct tggattaaga cggttttacc aactccggca
    12181 ccaccgaaaa gtccaacttt accaccttta aggtaagggg caagaaggtc aataaccttg
    12241 atccctgttt caaggatttc agaagaggta gacaactcat caaaagttgg agctttttta
    12301 tgaattggct gacgctctgc gtcttctgta aaaggagctt ccaaatcaat ggtatctccc
    12361 aaaacgttga agacacgtcc caaagtttct ttacctactg gtacagagat tggacgaccc
    12421 gtgtccaata cttccattcc acgagtcaac ccatctgttg attccatggc gatagtacga
    12481 accataccat ctcctaactc caaggctact tcaaggacga tttttgtttt tctttcgtca
    12541 tttttgtaga cgacaagtgc attgttaatc tcaggaagtt tttcccctgc tgcaaacaaa
    12601 acgtctacaa cgggaccgat aacctgagca attttacctg aactcatctc cttctcctat
    12661 tctatataag atactgtcgg ttcctagctc aactaggtcc taagttcatt ttcatacgag
    12721 ctggactaga gcctattcta aggcactagc acctgctacg atttctgtaa tttcttgtgt
    12781 aatcgccgcc tgtctggcac ggttatactg aattgtcaaa tcattgatga ctttcttagc
    12841 attatctgtc gctgtttgca tggctgtcat acccgcagca ttctcagctg tcttggcatc
    12901 gataatggca ccgtaaatca tactttctgc aaactgaggc aacaactgct ccagaatttc
    12961 ttctcggctg gtttccaatt caaaagtcaa gctgtactct tcatccgctt catttggatc
    13021 caagtcaaca atcggaagca tttgttccac acgcatttga ctggttagcg tattgacatg
    13081 gtggttgtag caaacataaa gctcatcaaa gagttcattt tggtacattt caacagtttt
    13141 tgaaataatc ttacgaactt gatcaaagct aggttggtct gccaagccac gtaattcata
    13201 aagtggttga ataccgcgag ccttaaagaa atcagctccc atcccaccga tacagatcat
    13261 ttcaaaacct ttaccgtctg ggtggtattc ttctttcaac tccataacag ctttcaaaat
    13321 agaggaatta taacctccaa ccaaaccgcg gtctgaagtg ataacgatat agcctgtctt
    13381 cttcacagaa cggctaatca acatcggatt agttgaagca ccagctccat taccatgaag
    13441 gatatctgtc aaaagtttac gcactttctg agcgtaaact tggaagttgc gagcagcttc
    13501 ttcagagcga cctaacttag cagccgatac catttgcatg gcattagtga tttgactcgt
    13561 attttttgtt gaggcgattt ttgttttaat atcatttaga gacactgcca tctgacacct
    13621 ctattcttat tggaagctag attgattgag aaactctgta atcgcagcat ccaagactgc
    13681 ttcttctggc aagtcttttg tatcacgaat ggtttccaaa atctctggat gttgagcatc
    13741 aaagaaggca tggaactctt cctcgaaacg aacaatatca tctactggaa cagtatccaa
    13801 gaaaccatgt gtcaaagcat aaagaatggt tacttgtttc tcaacaggta atggtttgtg
    13861 aacaggttgt ttcaagacct caacggtacg acgtccacgg ttcaacttag cctgtgttgc
    13921 tgcgtccaag tcagaaccaa acttagtaaa ggcttccaac tcacggtatg aagcaaggtc
    13981 gatacgaagt gtaccagcaa ccttcttcat ggctttgatt tgtgcagaac cacctacacg
    14041 agatacagat gaacccgcat cgatggctgg acgaatacct gcattgaaga ggccatcgcc
    14101 aaggaagatt tgtccatcag tgatagaaat cacgttggtt gcgatatagg ctgagatatc
    14161 tcctgcttgt gtctcgataa atggtagggc tgtaattgat ccaccaccaa gttcatcaga
    14221 aactttagct gagcgctcaa gcaaacggct gtggagatag aaaacatccc ctgggaaggc
    14281 ttcacgacct ggaggacgac gaagcaagag cgatagttca cgataagcta ccgattgttt
    14341 tgatagatca tcgtatacaa tcaaaacatg cttaccttga tacataaatt cttccgccat
    14401 agcaacccca gtataaggag ctaggaagag caatggagat ggttgtgaag cagaggctgt
    14461 cacaacgatt gtgtagtcca aggcaccgta ctgacgaagt gtttctactt gcgtacgaac
    14521 tgttgattct ttttgtccaa tcgcgacgta gatacagatc atatcttgat ctttttggtt
    14581 caagattgta tcaatcgcaa tggttgtttt ccctgtctga cggtcaccga taatcaactc
    14641 acgttgacca cgaccaatcg gtacaagggc gtcaatagct ttcaaaccag tttgcaatgg
    14701 ttctgaaaca gacttacgtt gcataacacc aggagctggt gcttctactg gacgagtttt
    14761 atcagtgtgg atttctccaa gaccgtcaac tggacgacca agcggatcca caacacgacc
    14821 aatcagactt tcacctacag ggacttccat gattttccct gtacggcgga ttgtatcgcc
    14881 ttcacggata tctgtaaagt cacctaggat gataatacca acgtctgttg actccaagtt
    14941 ttgagccata ccataagagc cgttttcaaa attcaacaac tctccactca tgacattttc
    15001 aaggccatga gcacgcgcga taccgtcccc gatataggtt acaacacctg tttcagtcac
    15061 atcaaaattg ggtttgaaat tttcaatttg ttgcttaatt aaagcgctga tttcttgtgc
    15121 gttaattgcc aaaagaacac cactttctat ttcaaatttt ctttaacaac tttaagttgt
    15181 tgtttaatac tcacatcaat tgtcttgtga ttggcaaaaa tgacaaaacc accaatgaga
    15241 ctttcatcga tttgttcttt tacactcctt actttcagag acattttttt ctcaatcaaa
    15301 gggagcaaac gagtcttctg ttcatcagtt agaggatgag cagacgtaat cgtcacttca
    15361 aatcgatttg tttctttttc aagtcggttc aagcaatcta caagcacatc ataaaaaaga
    15421 tttgctctgt gattgtaggc cagaacctgg ataaagtttt gtaataaagg cgacacagaa
    15481 tcttggaaaa aagcaattgt tttttcctta tccgactcgt ctactgccac ttgttttaaa
    15541 aaagaaggca gacctgtttt ttcaacaact tgcttgattt gagtcaagtc tgaaaagata
    15601 cggtcttctt ctcctttttc aagtaccaat tggacaaaag gcatgctgta tttttcaatt
    15661 acctttactg ttttcttgtc cattaagctt ctcctagctg atcgatatac tgatcaatga
    15721 gtgctttatg ggcatgactg tcaaggtttt gtgagatgat tttaccagct aagctgatgg
    15781 tcaaatctgc gacctcaccc ttaacactct gtaaagcttc tactttattt tgagcaattt
    15841 cttgattggc tttttctttt aagtgtcctg cttctagttt agcatctgct aagatattag
    15901 ccttactttg ctcagctgtt tcctttgcat tttcaatgat tgtcttagct tctttacggc
    15961 taccagccaa ttcatcttcg cgtttttgag ccaatacttc tgctttttga cgggcttctt
    16021 cagctctgtc aatatctgaa gcaatttttt cagctctttc ttcgaaaatg cctgtaatat
    16081 tagaccatgc aaatttttta atcaagacta gcaaaagaat aaaagagcca gtgattaaaa
    16141 taaaattacc aattaattca cctactgtta cgtgcatcag ttactccttt ctattcctct
    16201 tcaatattaa tcttattccc taaatacaca gatgtcaaaa gagtaaaaac ataagcttgg
    16261 atgcaggaaa taaagacaga aaatgcagtc caagccaaat tagctccaaa ggctactgga
    16321 taccaataaa tagcttggtg ggaaagaaga agtaacaaac tcgtcatgac ctctcctgca
    16381 aagatattcc caaaaatccg caaagccaaa gataagaagt ttgtaaattc ttccaagata
    16441 ttcatcggta tgacaaaaac aggactcata aaagatttta tacttttttt aaatccacga
    16501 cgacgaacgc tttctatatg tgtcaacaaa atgacaagaa aagataaggt taagtcatac
    16561 tgtaaattag cggttggcga actccaccag ttagtcccat cgatcgtttg aagctttgtc
    16621 attaagccaa ggttattggc aatcaccatg aaaaggaata aacaaaggaa aaagagtgag
    16681 taatctttca tgtagcgcga accaatgtta ggttctgtaa atccaataac aaagtcatag
    16741 acatactcaa gtacattttg ctttcctttg ggtttcaagg tcatattgcg gcttgcccaa
    16801 taaataaaga caaaaataac tcccacaatc aacaaagtca tggctaacat agtcagattg
    16861 aagataacag gaccaataga gatgattgga ttaatacttt cttccatcta aaatcctccc
    16921 ttttcttctc gttccatgta tttatttgat aataaatgag aagacaagag ttacaaagaa
    16981 agttccttca ataaaggcaa cacctaaaaa catcaaacta cgaaactcag aaagcatatc
    17041 tggttggcgt gctactgatt taaacagtcc attcatcaat aaaccttcac cgacagatac
    17101 gcccatacag gcaatacata agcctaaaaa tgttaaattc ataacaaatt ctcctttaaa
    17161 ataaaattta cttacttagt gtagtcctaa aatttaaatt tgtcaacttt taaccgctat
    17221 tggaagcgtt taataaaaat tatttccatt ttgctaaatt tcaagttcaa gttagccatc
    17281 cagaagtctt ctctgggtga cttgtagtcc aagcattttt taggatagtt gt
//
LOCUS       DAVBCW010000035        16998 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVBCW010000035 DAVBCW010000000
VERSION     DAVBCW010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16998
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.035"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(156..2249)
                     /locus_tag="VQ514_001005"
                     /pseudo
     CDS             complement(156..2249)
                     /locus_tag="VQ514_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397758.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
     gene            complement(2242..2790)
                     /locus_tag="VQ514_001006"
                     /pseudo
     CDS             complement(2242..2790)
                     /locus_tag="VQ514_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000059560.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2871..2933)
                     /locus_tag="VQ514_001007"
     CDS             complement(2871..2933)
                     /locus_tag="VQ514_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHP2/SHP3 family peptide pheromone"
                     /protein_id="HET2499777.1"
                     /translation="MKKISKFLPILVLVMDIIII"
     gene            complement(3102..4090)
                     /locus_tag="VQ514_001008"
                     /pseudo
     CDS             complement(3102..4090)
                     /locus_tag="VQ514_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050775.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4039..5166)
                     /locus_tag="VQ514_001009"
                     /pseudo
     CDS             complement(4039..5166)
                     /locus_tag="VQ514_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423473.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
     gene            complement(5329..6213)
                     /locus_tag="VQ514_001010"
     CDS             complement(5329..6213)
                     /locus_tag="VQ514_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HET2499778.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            complement(6325..7179)
                     /locus_tag="VQ514_001011"
     CDS             complement(6325..7179)
                     /locus_tag="VQ514_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HET2499779.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGIWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNLTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCNIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            complement(7237..7995)
                     /locus_tag="VQ514_001012"
     CDS             complement(7237..7995)
                     /locus_tag="VQ514_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET2499780.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEVILYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVYDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYALS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVVF"
     gene            complement(8057..8245)
                     /locus_tag="VQ514_001013"
     CDS             complement(8057..8245)
                     /locus_tag="VQ514_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HET2499781.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            complement(8567..9133)
                     /locus_tag="VQ514_001014"
     CDS             complement(8567..9133)
                     /locus_tag="VQ514_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499782.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKTYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            complement(9401..11080)
                     /locus_tag="VQ514_001015"
     CDS             complement(9401..11080)
                     /locus_tag="VQ514_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HET2499783.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIV
                     GEYEDAGKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDYGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEGRIQKAREGKWNG
                     GFAPYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAI
                     VSDEVWQAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKD
                     GTKYKDFYYYGCNHRQMIRGHKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEK
                     INMKVDTSEIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYR
                     MYDKVDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLISAL
                     ISEIQVYEEKQSNGQWLKSITFKLPIIEENLNIGLDNDEQVECVSLLEKRS"
     gene            complement(11172..11333)
                     /locus_tag="VQ514_001016"
     CDS             complement(11172..11333)
                     /locus_tag="VQ514_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499784.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
     gene            complement(12017..12331)
                     /locus_tag="VQ514_001017"
     CDS             complement(12017..12331)
                     /locus_tag="VQ514_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499785.1"
                     /translation="MGITWKDIEIDKEREGEIDKKSTPIFYGEFKNVRLSKEEYKNLK
                     EKLNSHTDIMINKLSRYMESSGKTYQNHYVTILKWYEEDKDKLRQKGLNKKMNYDVGE
                     SL"
     gene            complement(12297..12686)
                     /locus_tag="VQ514_001018"
     CDS             complement(12297..12686)
                     /locus_tag="VQ514_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2499786.1"
                     /translation="MPKRYLLDGKTPRISATNIDNGILGYYEDIDDKNYRVFENFISV
                     SFLGAVFYHKYKASLDMKIHCLKLKNKELNKEVAFYLTSIIRQALKNTEYKDQISSTV
                     LPDIKIKLPIDSRGTPDWNYMERYRDR"
     gene            complement(13074..13868)
                     /locus_tag="VQ514_001019"
     CDS             complement(13074..13868)
                     /locus_tag="VQ514_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2499787.1"
                     /translation="MKGLWSNNLTCGYDEKIILENINIKIPEEKISVIIGSNGCGKST
                     LIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGR
                     FPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSI
                     LLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKL
                     VAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHHVSS"
     gene            complement(13868..14875)
                     /locus_tag="VQ514_001020"
     CDS             complement(13868..14875)
                     /locus_tag="VQ514_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET2499788.1"
                     /translation="MQNLIIGIQKRKNRITLFSSLFLLIIISLSFFILLIGDESYSFS
                     TLIKVLNSETVPGASFSIMEIRLPKLLAGIIAGWSFGLAGFIFQTMLRNPLASPDIIG
                     VTSSSSIAAVFCILVLKTNSLTTGIISITCGLTSSLILFLLAKKDGFSAARLIILGIG
                     FQAVTRAGTSFLLLKVARYELQEVMRWLSGSLSFTKLDDIPLVLIVSIIATILVLFFN
                     KRLEIIELGEEIAIGLGANPELSRLVLIFCAVSLTAFSTSITGPIACISFLAGPIALN
                     IGKKRSPILAGLVGILLVLLSDIFSQNILPARYPVGVVTGLLGSPYLIYLLIKMNRRN
                     I"
     gene            complement(14875..15882)
                     /locus_tag="VQ514_001021"
     CDS             complement(14875..15882)
                     /locus_tag="VQ514_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET2499789.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLATFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVN
                     N"
     gene            complement(15872..16897)
                     /locus_tag="VQ514_001022"
     CDS             complement(15872..16897)
                     /locus_tag="VQ514_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HET2499790.1"
                     /translation="MKNKFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDH
                     AFGQTILDKKPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKE
                     LNGKANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQT
                     LWRDMIKIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAAD
                     TSKFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITY
                     GDDKTLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLG
                     NACKNAK"
BASE COUNT         5325 a         3215 c         2287 g         6171 t
ORIGIN      
        1 cctaaagaaa taccatattc aactggaaaa tctaaaaaaa ttgcaaatac tattccaatc
       61 atcaccccta gtgaggctgc tgttgaaatt acatattcat ttactttctt cattttcttt
      121 acacctcatt tcctttttgt atataagctg tagtactatc caccaagatc cattaagtac
      181 ttatgatttc ctgactcaac aatcttacca cctttcatta caaaaataat atccgcatcc
      241 ttaatcgttg acaatctatg agctataatt atttgagtac aatcctgact ttgtatatac
      301 tttgttattc tttcctcatt aatagtgtct aatgcactag ttgcttcatc taaaattaca
      361 atactaggat tatttattaa tgcacgtgcc agtgctatcc gttgcctttg cccacctgaa
      421 atatttgacc ccatctctga gatgatagta ttaaatttca tcggcatagc catgatttca
      481 tcatagattt gaactgcttt acaaacttcc tctatctttt gtgaagtaac ttcgttcttt
      541 aaagttatat tatcaagaat acttcggttc aataaaaatg aatcctgtgg aactactcct
      601 aaattttgac ttaatatttt tttatctatt tgattaatat ttacaccatc aaataaaatt
      661 tttcctgtat caatcttata taatcctgat agtatttttg ataacgtact ttttcctgac
      721 cccgattgac cgactagtgc aattctctgt cctggctcaa tagtcaaatt taaattttct
      781 attacaggag ctgagtcttt ggaataagag aaagataggt ccttaatatc tattctacct
      841 tctaaagaac actttttaag accattttca acattctctt cattttcaag ccagatatca
      901 tttactctat ttaagtatcc tgctgccaaa atatactacg tataagcatt aaatattgag
      961 atttctgaac taaataaaat tgatgccatc gtttgaaatg cgacaacctc acccaataaa
     1021 atttttccat ttaaaacatt atcaagtcca aaaatcaata taattatagg agcgaacaat
     1081 tgaaatacat tagtagctga attgtataaa atatgcacaa tacttcgttt tttatacttt
     1141 gtcaatacgt tttttaaata cttagaccag ttttcatata tttcatcttc taatcccgat
     1201 atttttattg aagaaatagt atttaatgct tctgtttcta ctgagtaaat caatgattgt
     1261 tcttgtaatt ctctattaac cgtatcatac attatttttc tagtactaaa cagaaacaaa
     1321 aaattgatta aactcaatat aagagctata attgataatg tttttgaact aagaaacata
     1381 tatattacaa cacttagaat agtcccgata tctactactc ccccaacaac ttggttagta
     1441 aatagctccc taaaaccact aagactagaa attctgtata aaatatctcc ttttgccctg
     1501 agttcaaaaa aactatatgg tagtttaagc aaatgcttaa atgtaaaact ttcaatatct
     1561 ctactaaaaa atatattaga aaatagaatt cccatactcc tagccaagat agaaataagg
     1621 tacaaacaaa gtagagaaaa taaaattaaa aacgaggttt gaaaagaaaa tgtattccct
     1681 aatgcagaat taataaactt tgaagtcatt actggtacgg aaagtattat taagtaactt
     1741 attattgaaa atagtaacgt caaaatcaat cgacctttta cctttgaaaa actagagaat
     1801 actcttatcc atggagaagg aaactgcgac ttcaaagatt gataattttc gttaggaaat
     1861 gatagcagta ctatattact aaaatgtttt gaaaactcct taaactcata atttatatat
     1921 cctttagaag gatccattat atagacctta ttctttttta ctttgtaaat aactacataa
     1981 tgttgattat cccagaatgc tattaatggt aacggaagtt cactaatttt atttacatct
     2041 ttaacttgat atgagccagt atcaaaattt cgtttattca ataactgttt caagtcacct
     2101 atactataac catctctccc tttttctata tcacgtctta aatctagtaa tgtctctttg
     2161 cttttataga aatgtagtat ggacaagcag caacataaac cacattctat ttgagacatt
     2221 tgctcaatat taggtacttt tctagtcatc atgtcctctt aactccagta ataaaacatt
     2281 aagtaatcta tcatcttgtt ttcgagatat ttcatagaca actccagata ctcctagcat
     2341 aaaagaaaat gtattcatag tattcaaatc cgtacttaat tctggtttga taacctgaga
     2401 aacgacatta tcaaaataaa aatctataga ttcttttatt aataggtttt cttcatcttt
     2461 caatattgca tcaagacaac tgatagtacc tacagaaccg tgacataaat tcaattctct
     2521 tattttttct acattagtta atacattttc aattattgat aaatcattat tcccaatatt
     2581 agttttatcg aaaatttctt ttctagctaa taaaatgccc ggagctccat aacaccaaaa
     2641 attaggaaag gaatcattat gatttcttca tatgtctttc caatttttta atatattatt
     2701 gaaacattta tcttctttat atagtaattc ctctatacat tttaatatat ctgtactagg
     2761 attaatttca taataatatg ataattacat tgctactcca gaataatcgt gtgctaaacc
     2821 tgttactgtt atattatcta attgtgaaat acaataatta atacactttt ttaaatgatg
     2881 ataatatcca tcaccaaaac caaaatcggt aaaaattttg aaattttctt catacttgga
     2941 cttcctttcc gtttttattt attgtacaat aaataaaaac ggatttaatt tttgttggga
     3001 aaatgggaaa ttatgaaatc aaaacttggc atcacactta gaaaagttcg taaaggaaaa
     3061 caaattagtc tatgctctgt agcagatgaa catctttcaa aatcacaaat atctcgtttt
     3121 gaacgtgggg agtcagagat atcttgtatt cgactaatca atattttaga caaattacat
     3181 attactttgt aggggagttt ttggctatcg ttgctcaatt gtctgattaa ggtcctaccc
     3241 ttgatgaaac aattattatc catgttttct ttattataga caaagtaaga agacgtttct
     3301 cgaatgtaga ctttatattt tttatgattt tcttcttcca taatatccaa ttgatagttg
     3361 ggaatgaaaa taagaccgct ctgtttgaca ccgaaagaca ccttgatata gacgccctta
     3421 tcaactagct tctctatttg gttctctgca agttccactt caaattcacg aacggtatct
     3481 catttttcct taaatgtctt aaaggcttcc tcaatctctt cagtggatac tttatcctta
     3541 tctcgttctt cttggaaagc atggtactgt tcctgtaaat tctctaatcc ttctgaagca
     3601 acgacttcct tatttttaaa ataatcttga aaaaatttga catcatataa tttcttatca
     3661 cttatttttt gatgacccaa acttatcttt tgattatttt cttccaggat aaaagttaca
     3721 ttttttgttt taagtcaatg gttagattca attcttttgc ttttgttatt aaatcttcta
     3781 aagaattgac acggtttaac aaaaattcta aacgactttc aatctcttgc ttagcaaaat
     3841 gcgttctaaa aaattcttca tcatatagat ctcgtttgct gagttggcgc cctcgaattg
     3901 gttttatcat cgttctatct gtcatcaaaa aacggctatg cttttgacta aaatcaatct
     3961 gaacatgcaa ctgctttgct ttctctaaaa aatcatcaaa cgatttagat tgctgaagca
     4021 aaaaataaag acgttgtttc aattcaaatt tatgactaga ttccttatat tttttataat
     4081 ctcgaaaaga tcaggatttt tagctaaaac tgccttacga aaatccttcc agaactgatg
     4141 gtcaatctca ttagccacat ccaaacgcca agcatcgata ttaaactctt caatccaata
     4201 agtcgcaacc tttaaaagat aattcttgac ctctggattg gctgtattta gcttaggcat
     4261 atagtcctcg aaaccaaaaa catgataggg taagtctctc ttattaacta gcttttcagt
     4321 tgtcaatggg aattgttgaa tatggaacca atccttataa gcagactgtt caccattttt
     4381 gacgacattt tttcattgaa gagattgcga accaatatga ttaaataccg catccagcat
     4441 gactttcatg ccacgatgat gcgcttgatc caccagttcc cgaaaggtct ccttgtctcc
     4501 aaaataacgg tcaatttcaa agtaatctgt cgtattgtac ttgtgattac ttgtagattc
     4561 aaagatggga caaagatata gtccagtaat acccaagtct tgcaagtaat tcatatgatc
     4621 aataatcccc tgtaaatcac caccaaagaa atcatcgctc ttaggtgtga cagatgaatc
     4681 ccagtctaaa gtcccttctg ggtttaatag agcattgcca ttggcaaatc tttcaggaaa
     4741 tatctgatac cataccgtat ttgaaaccca gtcaggaacc ttgcaggcat caatctcatg
     4801 aagataaggc aacttaaatc cattcccaat tgcatgaaga ttttctagag aattttccac
     4861 acacccttta tcgccataca aaatattttg accttctgta tctctgagct caaagagata
     4921 ctggatacgt gcaaagtcaa ctgacacttc aacctgccaa tggtcaaata aggtaccaga
     4981 agttatcttg accatttctt ttgtatcctg ataaaactct tccataaaga taaaagggtc
     5041 cccatagtgc aagttgatgc tttcaatgtc ccctttctta gttcgaattc gaatatggag
     5101 tttcttatcc ttataaagat aggcatactc cgactcaggc ctatggtaaa tagcactcaa
     5161 ttccatcaat ttgtctccta aagtattcat tcaaatttta cgcaaacgtt ttcaaactca
     5221 ataaatccag tatactattt ttgtaaacta tttgcaagga atgtccgttg ttaaaggaac
     5281 tatcttttaa agtatacaaa aagccttaaa aaaggctttc tctatatact aatcctcttt
     5341 cggttttgaa aatacttcta ctcgctgagc taggactttg atttctacag gtaggttatc
     5401 tgatttatct ccgtcaacat ctgactccaa ttcactatct gaagagattt taatattacg
     5461 cgctttgata tactcgatat tatcatttgc gacaacatcc ccttttaata aatcaggaat
     5521 gacggataat ttggagaata tagaggcatc tttcagaatc aaaatgttgg catagccgtc
     5581 cttgttttct tcaaagattt tcttatcagc gaagtaattt gtcaaaagga ccaaaacatg
     5641 gctagcttca ccaacataat ttccattttc tgtctcaacc ttaatgttaa agacctgatc
     5701 tgtcatgaca gacttcatgg tatttacagc ataggttaaa ataccgaatt ttgttttgtc
     5761 ctctatttca acattgtgaa tcgcctcagg cagagaaccg atactaaaga tataaccaaa
     5821 atagttgtca tttgctttac cgatatcaat cttattggtt aaatcaaaat ccagttcgtc
     5881 aattgcgcca tcgatgtctt gattgatttc caaaagtttt gtaatgaggt tacccgtacc
     5941 gcctgggata atccctaact taggaatgta gtctctctca tcaatacctg aaatgacttc
     6001 attgacagtt ccatctccac caaacacaac cactgcatca tactgctcac gagaagcttc
     6061 ttcagcaaaa tgtgttgcat ccagcgcttt ttcggtaatt ttggtttcaa catattcaaa
     6121 gtattctttt gctttattct ccagcttttc tttgtaatcc aaagccttct cgccaccaga
     6181 agtagggttg ataattacca ttgctttttt cattgatttt atccttaatt ttaaacagaa
     6241 atgtttacat ttcgtcgtat gcaagtaaat gtaatcctat tatacaatga aaatacagaa
     6301 aagagaaatc tgacgtactg gagattaata cgcttttatt ctattttccc atcgcctaac
     6361 tacatccttt aagggttcat ccaagtaaga ataggcctta tccttgatcc aatcaggaat
     6421 accgtaagct gcctctgcta tgttacaagt gattgctgcg agagtatcac tgtcgccacc
     6481 aagtgagatg gcatttctta tcgcatcttc gaagtctcta ctttcaagaa aggcgataat
     6541 ggcttgaggg acagtttcct gacatgtttc gttaaaacga tagttaggac ggatttcatc
     6601 taaagtttga gatagattgt aatcgtattc tttttctata taatccttaa ttcgtctttt
     6661 acactctgta agattgtcgt taattgattg ctcgtactct ctacaataac caccaaagta
     6721 aaaacgacat agaaagatag catcagctgt agccatagcg cctttgacac cttctggatg
     6781 attatgagtt acctctgcag aaagactcgt aagtcctcta gatgatggcc atataccagt
     6841 tttcgcataa aaaccacagt ccatgatcca agcacatgga gaaatacgca tagctgatcc
     6901 attcccaaag ctattataag gctcacggtt atcgctgttt agccatgcat taaaccgagc
     6961 accgtaatca gcattcggat acattctgcc atatttcttc atcgcgtcaa tgaaatcgtc
     7021 tttttgtcca ccattcataa ttgcttctgc aacagcacag gtcataaccg tgtcatctgt
     7081 aaaaaagcag tccttccgaa ataaaggaaa gtcctttgtt ttgatattgt tccattcgta
     7141 aacagaaccg acaatatctc caataattgc tccaagcatc agattcctcc ttgttcattt
     7201 tgatgctttt tatattggtt atataccata tttattttag aaaacaacat cctgttggat
     7261 tttaaaaatt tcattttttt caaaataggg ttttaccatt tctttccacc tagctctatg
     7321 aaaattgatt gattttaaag gagatagggc ataatttccc aatgcataac catcatttac
     7381 ttcaacaaca agtgttctgc catcgcgagt aacaccgata tctagtccat aagctattgg
     7441 cgcatctttc caacatgata tcgcttcatc aattacactt gcatcaaatt gtgcatgata
     7501 atcacctgta tagggtcgaa catctaatac gcgaccatct aacacaaaac aacgccattc
     7561 agctatgaat tctacaacct cactaatcca tataggatag tcgaaaggta gaccaatacc
     7621 tattaaatca tgggttccat taacaactct tccagtaaag acttttgaac cagctttagg
     7681 cttaataaat tttccccaat tatcaggtat attcacaatc tctcctaaaa taccagcata
     7741 aatctttcga ccataaaact ctttaagctc aataggatag tcataaaccg gaacgtttaa
     7801 gcccatcatt tttagtaatg ctctagtctc cattatataa tctacaacta tatcttcact
     7861 tgttaactct tttatttcag aaaaagattg atataaaata acttcttctc cttgtaagta
     7921 ggcacctact tgagcattgt atttattaat tgaaacctca cttggtaatt tactttgtct
     7981 aatataaaca accatttcat cactcctata tcactagtgt tacactgatt tgtaaaaaat
     8041 aatagcaatt ttgctcttat ttttttgagt aaatagctcc cataatatca tcgaaataat
     8101 caacggtatt taggagtaat tcaataacct gggactttgt tagtcgcatt ccccttctat
     8161 ctctagcatc ttctactaaa ttttcaagtt tctctagatt tttatcatcc aagctaatca
     8221 ttattctatt tttatcggtt gccattttca tcacctcaag ttaattctat cacaggtgta
     8281 acacttgtgt caactggctt ttataataca ttagtttaaa agtggagagg atttttaaca
     8341 cagtaacttt aaatctttgg tattaaaaaa ttttcacaat atttatagaa ataaaatctg
     8401 tctcaaatca gttatcaaat ctagtataaa ttatgagcgg ctactctaat actttccctc
     8461 taaacaagaa aaagacttac actcaaggat tttcttcccc cccttcgtta taacgttttg
     8521 actcttttac tagcaaaggt atatactcac aaggaacttt ggttgactat tgaatctctc
     8581 caacttcttc tttaacatat ccttctacat cttcaatctc tacaaacatt gggtctaagt
     8641 gacacaagaa atgccaaact tcgatccctt tttttctgta aagaatcgct tcaccgtctt
     8701 cacttccgaa aaagcttctg tcgatttcat atccgcggct ttctaagaag tcttttgctt
     8761 tacgatagtt cgtttctctt gtttcgacat aggttttaac ttcatggttg ttaacgacat
     8821 atgcatcaat ttttgaatat ccttcgatca ctctatcatt tttgagggat aaatttgaaa
     8881 tctctttcca aataatgttt acattttcct caggatcgaa cataaattta gataaaggaa
     8941 caatatttcc gttaaaaata atttccatat aatccggtat gtttttagga ttaaaatact
     9001 ccacttcaaa accatcttct gtttccagag tgtatcccgg gatttgagct acaaaggctt
     9061 tcccatcttc tatggaatca aatgctacta aatctttaga ataatcattt tggtacaatt
     9121 ccaatataac catcgataat ctctccattt tcattatcag gctaatgtaa ataagcacgt
     9181 cacctgacca attcaggctc tctgtatcat ctcatcatat ttcctactta ctttacgagt
     9241 cctataccca gaacacacct tatcgacctt cggtctcacc tcgttgcatt ggctgaacat
     9301 ctacttttac ttcgctgatg cttcaactcg tacaagcagt gataccgcct cagcgtgatg
     9361 cgtcagtggg actcaaaagg ttcggggaac cttttgagga ttaactacgt ttctctaata
     9421 aacttacaca ttcaacttgt tcatcattgt ccaaacctat gttgagattt tcttctataa
     9481 ttggtagctt aaaagtaatg gattttagcc attgtccgtt agattgtttt tcttcataaa
     9541 cttgaatttc agaaatcaaa gctgaaatta actgcctacg ctctacatca ttcatgactt
     9601 tatagagctt atcaaaatag atcagaacct tatatatgtt atctcctgta agcttttcag
     9661 cttcaatagt ctgtttcttt gctttcgcat caattagtga tgattctaat tcatctactt
     9721 tgtcatacat acgatatagt ctatcatcta aatcctgttt ccttctctta taatgcttat
     9781 cttcaacatc taaattatct atttcctcaa ttagcttaaa ctttgtagaa tgactctttc
     9841 tcaattcctt ttggtaatta tctatttctt tttctatttc agaggtatcc accttcatgt
     9901 tgattttttc ttgcatcata gaagcaaatt tcggattact tactatcttg acaatcacct
     9961 ctgcaacagc atcatctaac aattcttctc taatttgctt actgaatgta cacttatgac
    10021 ctcttatcat ctgcctatgg ttacaaccat agtaataaaa atctttatac tttgtgccat
    10081 ctttcttttt cttgatacac ttgttcccaa acattcccac tccacatatc gggcatttta
    10141 caattccaga aagcaagtgt gtgcgtgtat cttttccttt attcacatgc tcatatttct
    10201 ttgcttgaga ttttagctta acctgagcag cttgccaaac ttcatcggaa actatagctt
    10261 catgtatccc ttcagatatt agatattcat cttgttcaac ctgcttatat tcatttcttg
    10321 taccatgaac tttttctaaa gttcttcttc caaatgctat tttcccatta tatacaggat
    10381 tctttaatat ctttcttata agacctgcat caaacaaagg attcttacca ttctgtcttg
    10441 ggatttttct aattccatga ttctctaagt atttagatat cccattggct cctatcgtag
    10501 tatttacata ctggtcgaaa atcgttctta ttgcaactgc ctcttcctca tttataaaca
    10561 gcttgccgtc ttcaagttta tatccatacg gagcaaagcc accattccat tttccttccc
    10621 ttgctttttg aatgcgacct tccattgttt gaatacggat gttttctctt tctatttcag
    10681 ccacagctga taaaacagaa atcattagtt tcccagcatc tttagatgaa tcaatgccat
    10741 cttcaacgca gataagatta actccataat cctgcattat ctgaagtgta gaaagaacat
    10801 cagcggcatt tcttgcaaat cttgataact taaacacaag aacaaaagat actccatctt
    10861 ttccagattt tatatcttcc atcattcgat tgaactgtat tctaccttca atagacttgc
    10921 cagacttccc ggcatcttca tactctccaa caatttcata atcgttgtaa atagcaaaag
    10981 ctttcattcg tgatttttgt gcctctaacg aatacccctc tatctgtatt gacgtagata
    11041 ctcgtgtata gaggtatact tttatttttt cttttgacat agtattaatc tcaatataat
    11101 ttttctatat catatataat ttttttaatt taagtttgga ctatcatttc aagtatatta
    11161 taacactttt attagtccgt ctcaatttgt gtttttgcca tgtcaaaact atttttcatc
    11221 tcttgatttt ttgctggcgt tggatcgggt agattatcta aatctaaagc accagcatat
    11281 tttgcaatca gatttgctat taaatcagcc aatccattcc agtcattgtc caatatatac
    11341 ctcctctaaa gttttatatc taataattat ttgtttaatt aagttttttg acattgacaa
    11401 gtgctttgga ttagcaacat aggaatctca cctccgcctc tattccggat gagccggctt
    11461 caaccttaga agtatcatta tcctcatttt cttcatagcg gatagggtat ccctccctat
    11521 attcaaactc ttacttatcg ctcactttct ttttacttag cagaactttt tttgccgaat
    11581 tattcagccg aaagatcttg acggataggt tattacgctc caaaaataat taacgtcttg
    11641 tcttggtcta ttcaattgtt aaggttcaaa atttatcgag agttattaat ctttttaaaa
    11701 tttgaccatc agaaaatatt tatcttaatg taacaaaatt ctataaatta ccctcttata
    11761 cttaacagtg aaaagaagtc tttcttggta accaattttg aaatagaatt tgcttatata
    11821 aaaaggtcaa attcccactg cataagtagc agtgaaaatt agaccctctt ggtaactgtc
    11881 atctaaaagt cttctagcat ttctttaatt ttctaatcgc ctttaaagtg tcatatttat
    11941 atttttttat ctctggcata ctttgatacc tttttagact taaagtcttt aatagtgcct
    12001 ttccacctct ttttatctat aaagattctc ctacatcata attcattttt ttatttaaac
    12061 ctttctgtct tagtttgtct ttatcttctt cataccattt taagattgtc acatagtggt
    12121 tttgataggt cttaccactg ctttccatgt atctggatag tttatttatc attatatctg
    12181 tgtgtgagtt taatttttct tttagatttt tatattcttc tttgcttaac cttacatttt
    12241 tgaattctcc ataaaaaatg ggggtggact ttttatctat ctctccctct ctctctttat
    12301 ctatctctat atctttccat gtaattccaa tctggagtac ctctactgtc tatcggtaat
    12361 ttaattttga tatctggcaa tactgtgcta gatatttgat ctttatattc agtatttttt
    12421 aaagcttgcc taataattga agttaaatag aatgctactt ctttattcaa ttctttattt
    12481 tttaatttta aacaatgaat tttcatatct aggcttgctt tatatttatg ataaaagact
    12541 gctcctaaaa atgaaacaga tataaaattt tcaaaaactc tataattttt atcatctata
    12601 tcttcgtagt aacctaagat accattgtca atatttgtag cactaattct aggagttttt
    12661 ccatcgagta aatatctttt tggaatagat gagcctgttg gtacttgact cgatttcccc
    12721 tttttttcgg taataaatat ttctttttat tttgttgtct gatatttttc ctacctgtcc
    12781 tttgtaggat gagtattttc tagattttct tgaataactt tttacttgaa gttttagctt
    12841 ttgaactagt cgttgtactt tctttttgtt tattatcagt cctgatcttt ttaatattgc
    12901 tgttattctc tatatcctat ttttcattca tgatattctt ttactaattt tatcttaaat
    12961 tctgtgctgt atttgccatt aaaaaactga cctcctttag ttagtttttt ggcctaactt
    13021 ttgagggtca gttcaaaatt tgcgactttt aaatgaattc caatattcaa ttattaagag
    13081 ctaacatggt gcttgccaat aggaatcatt agaggcgaat tggaaatagg gtcacgtata
    13141 atttttgctt caagattaaa gatatcttta actagtttat catttagtat atcttcaggc
    13201 tttccctctg caacaagttt accttcttta attgcaaata ggtaatcagc gtatcttgct
    13261 gttagattta tatcgtgcaa aatcatgcaa atggttgtct tatatttttg gtttagatca
    13321 gtcaagaggt ctaatagttc tatttgatat gagatatcca agtaagtagt tggctcatct
    13381 aaaagtagga tacttgtatc ttgggctagg gctagagcta tccatactct ttgcctttga
    13441 cccccagaaa gttcttcaac taggttattt gctagatctt caacattggc cttaaccatt
    13501 gatctgttta ttatttcaag gtcatctttt ccaagactct taaaaggctt tctgtagggg
    13561 aaacgaccac ggcttacaag atcagctact gttattgatt cagggattat tggagattga
    13621 ggtaatatag ctatgtgttt tgctaaatct ttttctttat aagaattaat tgatttatta
    13681 tcaagcaata cttctccctc taatggcttt ataagtcgag acaaggtttt aatgagtgtt
    13741 gatttcccac aaccatttga cccaataata actgatattt tttcttcagg tatttttata
    13801 tttatatttt ccaagattat tttttcatca taaccgcagg taagattatt tgaccacaga
    13861 cctttcatta tatattcctc ctgttcattt ttattagtaa gtatattaag tatggtgaac
    13921 ctaacaagcc agttacaaca cctactggat atctagctgg taaaatattt tgagagaata
    13981 tgtctgataa caaaactagt aaaattccaa ccaatccagc taatattggg cttcttttct
    14041 tgccaatatt taaggctatg ggaccagcta aaaaagatat acaagctatt ggtcctgtaa
    14101 ttgaagtaga aaaagcagtt aaagatacag cgcaaaaaat taaaacaagc cttgaaagct
    14161 cgggatttgc tccaagtccg attgctattt cttcaccaag ttcaataatt tctagtcttt
    14221 tattaaaaaa taaaactaat atagtagcaa taatacttac tattagaaca agaggtatgt
    14281 catctaactt tgtaaaagat aaagagccac tgagccatct cataacttct tgtaattcat
    14341 atcttgctac tttcaacaat aaaaatgagg tgcctgctct tgtgacagct tgaaaaccaa
    14401 tacctaatat tatcagtctt gctgctgaaa aaccatcttt tttagctagt aaaaataata
    14461 ttaaagatga tgttagtcca caagttattg aaataattcc agtagttaaa ctatttgttt
    14521 ttaataccaa tatgcaaaag accgctgcaa tagatgaaga acttgtgaca ccgattatat
    14581 caggacttgc aagaggattt cttaacatag tttgaaagat aaatcctgcc aatccaaaag
    14641 accagccagc tataattcct gctaataatt ttggtaatct aatttccata atcgaaaaac
    14701 tagctccagg aacagtttca ctatttaaga ctttaatcaa agttgaaaaa gaataacttt
    14761 catctccgat aagtaaaatg aaaaatgata gactgattat tattaataaa aatagtgagg
    14821 aaaatagtgt tattctattt tttctttttt gaatacctat aattaaattt tgcattagtt
    14881 attaacccct ctatttttca tagttacata aataagtact ggacccccga ttattgcagt
    14941 aattatccct acttcaattt cacctggttt acctaacata cggccgatta tatcacatat
    15001 aagcaagagc tctgcaccta taaaagatga agaaatggtc attgtgcgta tatctttgct
    15061 tataaataag ccacaaaagt gaggaactat aagacctacg aagccaatag gtccaccaat
    15121 tgcagtaata cttgaacata aaagcacact tgcaattatt gcaagtgatc ttatcctatt
    15181 aacattaact ccaagaccaa cagccatttc atcacccata gctaaagcgt ttaaatctga
    15241 tgaaataaat atagctatca agtgacctaa aattataaaa ggtagtagtg tagatataga
    15301 agataatgta gctgctccaa ggctacctat ttgccaaaat ctaaatttgt ctaagacgtt
    15361 attattcggt aaaattaaaa aacttacaaa actgcttaaa gccatactaa cacaagttcc
    15421 tgataaggct agttttatag gggtaaggcc tgcttttccg cttacagcaa tcgcgtatac
    15481 aaaaattgca cttactaagc caccaatgat tgcaaaactt atatggctta tgcttgatga
    15541 aattcctaaa aaagaaagac caattactac actaagactt gctcctgtgt ttataccgag
    15601 tatacctgga tcagctattg ggtttctagt aactgattgc atcaatacac cgcttatggc
    15661 aagactagaa cctgctaaaa ttgcaaaaat agttctaggt attcttttat atataattga
    15721 tttaataaaa tcatcatttg tattgcccat tccaaaagtt gctaaaaaat ctctgatatt
    15781 aatttctttt gttccaagct ttaatgacaa aaatattgta ataagaagac ctaggcagat
    15841 tacaaaaatt atgcctaggt ttatttgttt tttatttcgc atttttgcat gcatttccta
    15901 aaagatttag gtattcttca atagtatagt ttattgaaag tggtgttgga gtgcatgagg
    15961 ctgctaacgg tgtattatct ggaattacag caacggcacc atttttaatt gcatttattt
    16021 tacctaaaag aggatctttt tgtaaagctt caagagtttt atcatcacca taagttatga
    16081 ttacatcagc atcatttatc ttatttgctt cttctgcaga aatttccttt gcaaaactat
    16141 cttcactctc aaattctttt aatgattcag ggaaaactag acctaaatct gttaaataat
    16201 ttgctcttgg atctttgcta gtataaatcc agaattttga tgtatctgca gcattaatca
    16261 tagtaaataa tacttttttt cctttgattt ttcctttgat ttctggatgt ttttctaatt
    16321 ctttggatat acgagcttca gtatttttga ttaactcatc accttctttt tccataccta
    16381 aggcttttga atcaatttta atcatatctc tccataaagt ttgccaaggt ttagatttgt
    16441 atgctgctac aggagcaatt tttgatagag tgtcataatc ttctttagtt ataccagaat
    16501 aacctgctaa gataacatct ggtttagaat ttgatattgc ttcaaagtta agtccatcca
    16561 aatcgtcaaa taggttagct ttaccattta gttctttgat tttttcttct gtccatggta
    16621 aaactccttt atcagcactt acaccgtaat ttgcttttga aaatccaaca ggaactattc
    16681 ctaaagctaa tgctacatca tgatttcccc aagcaatagt tgcaactctt tcaggttttt
    16741 tatctaatat agtttgaccg aaagcatgat ctaaaactat tttatcaggc gcatgctctt
    16801 tagaagtatt ttcttcattt ttaacagaat tagaagaaca agcgctaaaa actatacaca
    16861 tagctaaaat agctattaga aaaaatttgt ttttcataaa aactccttaa acatatttca
    16921 agtctattgt atcgataata attatcattg tcaatagata cagttacaaa tttaataata
    16981 taattttttc aaaaaagg
//
LOCUS       DAVBCW010000036        16931 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVBCW010000036 DAVBCW010000000
VERSION     DAVBCW010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16931
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.036"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(136..1116)
                     /locus_tag="VQ514_001023"
     CDS             complement(136..1116)
                     /locus_tag="VQ514_001023"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HET2499791.1"
                     /translation="MFISISAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGIIFYLFYERGGDILSVFGYPVHLGFFYIFFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            complement(1118..3370)
                     /gene="pbpX"
                     /locus_tag="VQ514_001024"
     CDS             complement(1118..3370)
                     /gene="pbpX"
                     /locus_tag="VQ514_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HET2499792.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGLT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKAEEVPDMYGWTKATAETFAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            complement(3374..3691)
                     /gene="ftsL"
                     /locus_tag="VQ514_001025"
     CDS             complement(3374..3691)
                     /gene="ftsL"
                     /locus_tag="VQ514_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HET2499793.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            complement(3703..4653)
                     /gene="rsmH"
                     /locus_tag="VQ514_001026"
     CDS             complement(3703..4653)
                     /gene="rsmH"
                     /locus_tag="VQ514_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HET2499794.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNNYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIHQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            4819..5013
                     /locus_tag="VQ514_001027"
     CDS             4819..5013
                     /locus_tag="VQ514_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2499795.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            5029..5571
                     /locus_tag="VQ514_001028"
     CDS             5029..5571
                     /locus_tag="VQ514_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HET2499796.1"
                     /translation="MKKETFTEKLIKRTYGISGPLDEYKRREADSIGNQVFIILFYLM
                     IFGNLIPLLLAYKYPQEVALIYPPLILVIALIAAGYVTYQMKKTGITVIEPDMLNEKE
                     SKQLHYPGLKAGLFFGLWMFFITPLLSILIDEGQDYFHSLLTIRNGVSSVLGSIFFGA
                     SIQFLISRRIAKTKKNQDED"
     gene            5582..5824
                     /locus_tag="VQ514_001029"
     CDS             5582..5824
                     /locus_tag="VQ514_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499797.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            5835..6170
                     /locus_tag="VQ514_001030"
     CDS             5835..6170
                     /locus_tag="VQ514_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499798.1"
                     /translation="MKSLARLLIIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTTPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(6280..7281)
                     /locus_tag="VQ514_001031"
     CDS             complement(6280..7281)
                     /locus_tag="VQ514_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2499799.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKKLNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(7341..9242)
                     /locus_tag="VQ514_001032"
     CDS             complement(7341..9242)
                     /locus_tag="VQ514_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HET2499800.1"
                     /translation="MKEERRQFFERIDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWSYMLNRQEYLLQFMIGYLVEGDKDYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSYYRAKYTLSNWGILQTIPMLAIYHFFSDKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYQELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEILSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     RRAWQPKSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGVHY
                     IEGAYWSAEPDLPYLHRRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDIGVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            complement(9264..9557)
                     /locus_tag="VQ514_001033"
     CDS             complement(9264..9557)
                     /locus_tag="VQ514_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET2499801.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIKDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            complement(9557..10375)
                     /locus_tag="VQ514_001034"
     CDS             complement(9557..10375)
                     /locus_tag="VQ514_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET2499802.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPTKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFALLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            complement(10362..11141)
                     /locus_tag="VQ514_001035"
     CDS             complement(10362..11141)
                     /locus_tag="VQ514_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HET2499803.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            complement(11156..11647)
                     /locus_tag="VQ514_001036"
     CDS             complement(11156..11647)
                     /locus_tag="VQ514_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HET2499804.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNATGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            complement(11658..12848)
                     /locus_tag="VQ514_001037"
     CDS             complement(11658..12848)
                     /locus_tag="VQ514_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HET2499805.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSAQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            complement(12860..13294)
                     /locus_tag="VQ514_001038"
     CDS             complement(12860..13294)
                     /locus_tag="VQ514_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2499806.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
     gene            13565..14380
                     /locus_tag="VQ514_001039"
     CDS             13565..14380
                     /locus_tag="VQ514_001039"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HET2499807.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
     gene            14399..15040
                     /locus_tag="VQ514_001040"
     CDS             14399..15040
                     /locus_tag="VQ514_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HET2499808.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKGYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            15071..16072
                     /locus_tag="VQ514_001041"
     CDS             15071..16072
                     /locus_tag="VQ514_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HET2499809.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITVAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            16082..16711
                     /locus_tag="VQ514_001042"
     CDS             16082..16711
                     /locus_tag="VQ514_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HET2499810.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSALSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYVTLR"
BASE COUNT         5015 a         3787 c         3080 g         5049 t
ORIGIN      
        1 ttagaagtag atgtgtacta ttctagtttc aatctactat attttgagca gcctaaagtt
       61 agctttctag tttgttcttt gattttcatt gtgtatttaa atacaaaaac accctgaaac
      121 atcaggatgc cattcttaca ttaaatacaa aattgctagg gtcagaagac ttgctagaag
      181 tcccactccc caaaagaaga agtcaacctt ccactcgctc caaggatttc ctttaccaga
      241 caatccccca agctcaaaat ggtgatgtac aggcgtcata cggaaaatac gtttaccacc
      301 tgtcagtttg aaataactga cttgcatcat aacagaagtt gtttcaaaaa catatacaat
      361 tccgataatc aagagagtcc attcttggtg gagagccata gagatagctg ccagcatccc
      421 acctagggcc aaacttccca catcacccat aaagaccttg gcaggcttat ggttaaagat
      481 gaagaaaccg agcaaaccac caatcatggc aagaatcact agaagaatat ccatctgacc
      541 ttgcacatag gcaataactc cataggcaga caaactaatc acaacggaaa tactagctaa
      601 accgtcaaca ccgtctgtca agtttactgc gtttgaaaaa ccgactagcc agaaaagagc
      661 gaagaaaata tagaaaaatc ccaaatgaac tggataacca aagacagaca ggatatcgcc
      721 cccacgctca tagaaaagat agaagataat tccacctaga agctgaagag ctaatttttg
      781 cttaggatta agcccctcat tgattttacg aaagaccttg agaaagtcat ctaaaaatcc
      841 gaccaagcca tacaagacta atatgaacaa aatcattccc acattattgc tgaattggct
      901 actaaatagg gcgaaaaaga aagcaaccaa aacagaagta atcaagaaaa ccaaacctcc
      961 cattgtagga gtcccagctt ttgcctgatg ctgtttgaca tcttcatgca tctgctggcc
     1021 tgtaatttgc gcctttctat aaaattggat aaaggccgga attcctacta aagttagtaa
     1081 aaatgtcaca attccagcac tgatggaaat aaacatatta gtctcctaaa gttaatgtaa
     1141 tttttttaat gtccttgata gctgtgttag cacgaacatc ttgcttctgc acagtagagc
     1201 ccgaaccttg aaattcaagt tctatattga gccacttagc aaaggtctca gcagtcgctt
     1261 ttgtccaacc atacatatct ggaacttcct ctgctttatc agataagata aggacttgct
     1321 ggttcggggc aagattcttc ccttcttcag cagaactgtt tttaatcttc gttcctgttc
     1381 ccacaacgat gggttgtaca agattgcgac gcaattcttc tgctaaatca ccaggtgaaa
     1441 tatccttgac actaggcata ggataaggac tttgttgact tacttgctcc aaagctttag
     1501 ctgttgtttg aagattgaga gagtctttca tagctgaagc ccgctccaag ataggattgg
     1561 caaattctcc caactgaata cctgaataat gttcaggttg ttggaccgtc acatacaaga
     1621 taaaatcagg attttcagcc ggactcatcg atacagccga gaaaatatag tcggttaacc
     1681 cgactagata accaccattt ttctcgtcag caatctgagc cgtaccagac ttgagggcta
     1741 cattttgccc aggaacagtt acagttggct tgcctgtgct gtggttatac atggttccat
     1801 aaaccggatc cgtccctacc aaaaccatgt tagtccgagt tagactagct gcatctttag
     1861 aaacaggatt tcccacaatt tctttttgag atttccgagc agtttgatca tttggatcat
     1921 aaatggcact aataaattta ggctccagca tgacaccgtc attagcaata gctgtaaagg
     1981 cacgaatcat ttgtgtctgg gtcactgaaa tcccttgtcc aaatgaactt tgcgcaatgt
     2041 tgacaatatt atccgcagga agctgaccag catactcatc cgtcaaaccg aaacgggtcg
     2101 gaactccaaa tttaaaacga ttaagataat caagccaggt agcatctccc atcttttgct
     2161 caaggagggt catcccaacg ttacttgagt gtgcaaaacc ttgagaaaaa gtcatcattc
     2221 tgccaccagt caatccttca ttaacgtccc aatctcgaat cgtggcatct gcaattttta
     2281 actcactact attaaagact tctcctcctg gaaaggtatt attatcaata gcagcagcca
     2341 acatcatcac tttcatagtg gaacctggct catagttact ttggtaaagg atatcacgcc
     2401 aaacaaagtc ctctgtaatg ccttcttttg tatctgcatc aaaggtcggt cgttgcgttg
     2461 ttgccagaat ttcccctgtt ttagcactga ccaaagtcgc tgtcatgtac tttcctttta
     2521 ccttctcttg aaaagcatcc atctgggttt ccataaagga ctggagaggg ctggaaatgg
     2581 ttgtataaac atccttaccg tccatcgttc gttgggaaac ttgttctgtt ccgggtacaa
     2641 tattacccag acgatccttt tcataggtaa taatgccgtc tgtccctgca agaatactgt
     2701 tcaaggaact ctccattcca gaggttccca gcaagctctt gcttccatct tcattttcat
     2761 ggagctgagc taggccgata aaactagaag caaattgtcc gtttgggtaa ctacgattgg
     2821 gactggttgt aaaatcaatc cccttgacct ctgcagcttc caattctttt ttgatagaca
     2881 tcatattggc ataggtaatc ccatttccct ttgctccaaa ggaaacttgc ttgagattag
     2941 gttgcgagag ttgctctctt acataggatt cttccatgtc cagatactta tgaaagacct
     3001 ctgcaacctt gttaaattgt gttttttcta cgtaaagaat cttacccgtt gctgacttat
     3061 agttctcatc aatgaccgca tagacattat aggaggttgc atcctcagca atcgggactc
     3121 catttcggtc ataaatagtc ccacgtttgg caggaactgt acgggtggtt tgatgaacct
     3181 tcttagcttc cttcgctaaa tctgttccaa agcgagtgcc tgtcccaata atgaccgcaa
     3241 aattgactaa aaaaatggca aaaacaaaga cagataataa actcagactt tttccaactc
     3301 tgcgtctgtt ttcagccggc gatttccgat ttttggtcgc ataacggatt actctttttg
     3361 tccacttcat atcttactcc gctattctaa tattttcatt gtttaattgc aaatcgtgtg
     3421 aattggcaat ttctttcaaa cgttctgcac gtaatagttc attgacctct tgcttggcat
     3481 cgtccaattc ggttttcttt tcctctatct gcgcattgat ttttgtcaaa tcattctgca
     3541 cttgcaagag cttggtctgc ataaaaataa tactaatggc tacaataaga gtggttacag
     3601 caatggaaaa gtaaaaagct ttttccacac gcgaaaaccg tttaagttgc atctgtagta
     3661 tttgacctgt tttttccatt ttttctgcca tctttttccc tcttacttgt gaatttttct
     3721 ggccacgcgc aacttggctg agtgcgagcg gttattggct tctaactctt ccgcacttgg
     3781 caagattggc ttacgggaca ccaattccat cttgggcttg agatcatctg ggatgaaagg
     3841 caagcctttt ggaacttcaa ctgttgaagc ttccttgaac aattgcttgg tcaagcggtc
     3901 ttctaaggaa tgaaaggtaa tcactgaaat tctaccatcc agagccaaca tatccatagc
     3961 ctggtggatg gactcatctg ccgcccccag ttcatcattg acttcaattc gaatagcctg
     4021 gaaaatctgc ttagcaggat gccccttctt cttgagttcc ttggcaggtt tgaccaactt
     4081 gataatctct gctaactcag tcgttgtctc aatcggcttc acttcacgcg cttgctcaat
     4141 cttacgcgca atctgtttag agaatttgtc ctctccatac ttgaagaaaa tacgaaccaa
     4201 gtcatgatag tcataattgt tcaccacttc atacgctgtc agactagcat cctgattcat
     4261 ccgcatgtcc agtggcgcat cctttttata agaaaaacca cgctcacgct ggtctaattg
     4321 aggactagac actcccaagt cataacaaat tccatcaatt tcctgaacac cagcttcgcg
     4381 caaacgtgcc tgtaaatgac ggaagttatc cttgataaag gtcaccattc ccttctcaat
     4441 gtaaggtgcc aagcgttttt gcgcattatc aatggcattc tgatcctggt caaaggcata
     4501 gagatggcct ttttcactta atttacttaa taaatactcg ctatgtcctg ctccgcccaa
     4561 agtcgcatca acgtagatac cgtcaggctt tacgtcaagc atatcaatcg tttcgtggag
     4621 taagaccgtt acatgatgaa attcttttgt catatcttat ctattttacc acaaatccga
     4681 ccagcttgca cttgtcagac aaataggaca aggagactac tatttcttca aaaaatatgt
     4741 caaaaatact tgacacttta tgtttaaaag actataatat agtcaaatat atacgacata
     4801 aatcagaaag gagaacagat gaatcgtgtg aaagaatttc gcaaggaatt gggtatttcc
     4861 cagctcgaac tcgccaagga tatcggtgtc tcgagacaga ctatcaatat gattgaaaac
     4921 gacaagtaca atccaacctt ggaactctgt ctcaatctcg cccgcagcct ccaaactgac
     4981 ctcaacagtc tcttttggga ggaagatttt taaaaaagga gcaaacttat gaaaaaagaa
     5041 accttcactg aaaaactgat caaacgcaca tacggtattt ctggtcccct tgacgaatac
     5101 aaacggcgtg aggccgatag tattgggaac caagttttta tcatcctctt ttatctgatg
     5161 attttcggaa atcttattcc actccttctg gcctataaat accctcaaga agtggctcta
     5221 atctatcctc ctctgatttt agtgattgcc ctcatcgctg ctggctatgt cacctaccaa
     5281 atgaaaaaaa caggcatcac agtcattgaa ccagatatgc tgaatgagaa agaaagcaag
     5341 caactacact acccaggtct gaaagcaggt ttgttctttg gtctatggat gttttttata
     5401 actcctcttc tcagtatact catagatgaa ggtcaggact attttcattc tcttctcact
     5461 ataagaaatg gtgtatcaag tgttctcggt tctattttct tcggagcgag catacagttc
     5521 ctcatctccc gtcgcattgc aaaaactaag aaaaatcaag atgaggatta ggaggaaact
     5581 catgaaaaaa gaaaagaaac agttacgtta tcctggtctg aaagcaggtt cgatttatgg
     5641 aacagtgata ttttttataa ttccactcat tgatacccta acaagtgaaa atccaaattt
     5701 tatcagttct cttctaaata caaaacatat ttttaaaact atactgggag ctttcttttt
     5761 cggagtgatg atacatattg tcgactcact tcgtattgca agagctaaaa aagaccagga
     5821 ttaggaggtg ccttatgaaa tcactagcta gactactgat cattcatgtt tttatcagta
     5881 ttttcctttt cttcgccctt atttcaggag ctgtttctca tacagtttta ctactcctac
     5941 tcctctttct tcctgcgctc aataaaggac ttgagaaaat acaatcaaaa cggatacctg
     6001 tcctcaacgc agccctcttc tttctcctca tatcctttcc acaactttta accacccctg
     6061 tccaatggaa attttcaata ttcctagtcg taaccatcat ttcaagtttg gcctacttct
     6121 ataactttta tcaagtagtt aaagaagtag atcaaaaaca gttgatttag gagcttgctc
     6181 ccataatttc tcatttcata ggcaaataat cccacctagc ttgttctagg tgggattatt
     6241 ttatttctta acagagattg caagtcattt tccttcattt tagaaagtcg actctttcca
     6301 attcacacta caatccaaga cttgttgcct ttcttcttga ttgcggcctt caatctggtc
     6361 aatcaaaatc tttgtagcct gttgtccttc ctcaaaggag ggctgaacca gcgtcgaaac
     6421 acttggagaa gaaaagcaag tccactccgt attgtcaaaa ccaatcaacc caacttgtgg
     6481 caagttataa ttcaactttt tgataacggt aaagactaga ggtagggccc aacagttagg
     6541 gataaatacc agagtttttt catcgggatc gatttctttt tgtaaaaatt ccttaatttg
     6601 ttccaaattc gtatgcttat cttcaatggt tagactggcg tgacgcatat tagcatctgt
     6661 taaagcatcc acaaaaccac ttgcccgctc aatccgagta ctcaaacgac tcgtatccgc
     6721 tgtaatcaag agaaaatgtt cataaccttt ttcgatacag gactgggtca tgtcataaac
     6781 ggcatcatag ttattggttt taacccagct agtccggtgt tcatagagct gactatcaaa
     6841 aaagaccatt ttcttctttt tctcatcgat gatacgagaa tattttcgga aattagaggt
     6901 cggctgaata ataaagccgt ctactcccaa gagaagcatg ctttcaatat accggtcctc
     6961 actctcttgg ctgtaattac tatttcctat cattacctgg tagccattct ggctggcgat
     7021 atcctcaatt cccttaacaa tttggtttga gaaactgttg gtaatatcac cgatcaaaac
     7081 accgattaat tttgttcgtt tggagtttaa gctacgcgca acaatgctcg gtttgtaatt
     7141 tgtttcatga ataacttttt caatcttttc acgtgtctct tgggacattt tttcatattt
     7201 cccgtttagg taaaatgaca cggttgtttt cgaggtctga gccatttccg caatgtcttt
     7261 tatggtcagt ttcttctcca atctaaaaca cctcctacta atacttatta taacatgttc
     7321 actattcaaa atgcctattt ctagcactgc aagcgaatca ttctctcatt tattttatca
     7381 tatactagac attttccacg catttttgtt ccgtcaacga ggcacaattt ttcacctaca
     7441 ccaatatcct cgcttaataa cagaatcagc ttgtctgttt cgtcattttt cacttcaaaa
     7501 gccagagcat tttccacttc acgatcatct gtctggtaga cagaatgacg gatgatttca
     7561 aaactctcat gcgcaatgat ggtataatcc agcatctgat tctcaaagaa ttggcgcttg
     7621 gtgagtttgc tacttctttc aagctcattg tatttaggag aaatgatggt atcttccaaa
     7681 tcaaaatcaa cttcactcca tagtctcaac tgattgattt tcccatcttg ataggtcaca
     7741 tccttgtcaa ggataaactg agtcaacgcc tcatgctgac cttgacacct gatgtcatcc
     7801 accaagagcc agacatcctc taccaacatg aggatttttc ttctgtgaag ataaggcaaa
     7861 tcaggttctg ctgaccaata agccccctca atataatgca ctccctccct ttctttatgg
     7921 tgacaaaaca gggagtgagg atagtattca tattcccagg atcctgtgat tctttccgga
     7981 gctttcccat ctacaatgca ggtcgaatga ctccaagcac tctttaagag ataacgttca
     8041 tatatctccc gataagaata acgcccagca tctatgaaaa taggttggcc ttgatactgt
     8101 aagcaaaaac tattctcgtc actatggcta tgggcacttc ctagcggacc atttttgaaa
     8161 aatagataac gatgttcatc cttaatacag acatgtccag agtcttcaaa gatcatggac
     8221 ttaggctgcc aagctctcct ttcaaattcc tgcagtcgct tgaccttttc tcgccccagg
     8281 aacaagaggc taagcaaatc aactttaaca tccagaccgt taagaaggtc ttccttgttc
     8341 aaaaccacag cagacaggct caaaatttct gtcgtttctg tagaatcgct atcaccaaaa
     8401 gccaaagttc gtccatctaa gcctgtcatc atttgaatat aggtcgccat cttttccagc
     8461 aactcttggt aactatcttg caagtctgga agcaagagac acaaatccag caaggcttta
     8521 taaacctcta catgatagag aatcgactgt tcaaactggc ttccatctcc taaaatctgt
     8581 gtctcaattt gctgtttcaa ctcctctgaa gcaaaatggt aagcttcttc taggtccatc
     8641 ttatctgaaa agaaatgata gatagcaagc atcggaattg tttgtaaaat cccccagtta
     8701 ctaagggtgt acttggcgcg atagtagctt ttcataaagt caatctgctt ttctagactg
     8761 accaaaattt tctctagttc tttctcctct agcaagtcaa atttcaagag aagcaagagt
     8821 agtttcaacc aagtaaagga acgaataccc gtatccaagg ttctagtcat caaggattga
     8881 ggagaaaatt ctctcacctg ctcaatccaa tcaaatagaa agaacttgca cttttgaata
     8941 tagtccttat ctccttctac cagataccct atcataaact gcaagagata ttcttgtcga
     9001 ttgagcatat aagaccattc tggatcatct tcaaatactt gatcccatac catcggctgg
     9061 atttgatgga tttttgaaca aggctccata tcccaaggac tatcaaacat aaaacgattg
     9121 tccatcaagc gttcaaggga actcttgact ttctcatagt cttttgaaca gtgggacaag
     9181 atataatcac gacattggtt tccatcgatt ctttcaaaaa attgtcttct ttcttctttc
     9241 attatctatt accagaaaaa gaactactta aaaagcagtt cttttgtctt tcccattaca
     9301 ctttcctttt ctacatagat gaccacacct tttgcaatct gcaaggagac caagtcatct
     9361 ttgatagaaa tgatttttcc atgaattcca gacaataaca acacttcatc accagatgtt
     9421 aaagaagcta aatactcttg tcgttgctcc atctgtttgc gaagcaactt ttgctgacga
     9481 atagaatgaa agcttgacag taaaagggga ctcactgcca agacaatcac tattccataa
     9541 aacaatgttg tatccattaa gctataatct taagccagct tccgataatt ccgatgataa
     9601 ctgttaaaat aacgagttta tatgttgtcc atttcttttc tttgatcaag tagtaaacta
     9661 aaagtgcaaa tagggctggt agaagagctg gagcaacctt atcaagcatt ccctgaatac
     9721 ttacgatact ttgtttagcg tctgctttaa cttcccctgc agcaaaggta atcggcacca
     9781 taatcttaac agatgtcgct gccaaaccag caattacggt tacaccgata atattggcaa
     9841 tacgagaaat cgttgccatc tgttcgctta gtttatcaat cacagttgtt cctagtttgt
     9901 atccatacag accagttgac aatttaatcg ctgttaaaat cgtattcatc gcaaggaaga
     9961 acaagattgg accgacaacc aagccttctt gagcaaacga agctgcgatg gttgagaaca
    10021 atggagctaa acagaattga gaaagagaat ccccaatacc tgccaatggt cccatcaagg
    10081 ccatcttgat gctacgtgtt tcttttgttg gtcggccatt ttccaacatt acaagatgca
    10141 agctggtaat aaaaggcagg aagtgtgggt tggtattata gaattcacag ttttcttcca
    10201 aggcttggta gaaaccttcc tgatcctctc catagtgttt tttcaaagca ggatacatca
    10261 cattggcata tcccaaccct tgatagttac tatagttaaa tccattttga caaaagaatg
    10321 cccgcaaaga cgttttaaga taatcacgtt ttgttaattt gttagatcca gtcatcgtgt
    10381 gcttcctcct ctaccacatg atccgctgtt tttggcttgt tataaaattc aatcaaagca
    10441 aagatagtac ctacaattgc aataccaatt gttgggatgt ttagataagc tgcacaaaca
    10501 tatcccaaca agacaaaggg aatcaactct ttcttagcca tcactgacaa gatcatcgca
    10561 aaaccgatag ctgggagcat tttaccagca actgtcaaac ctgtaagcaa tactggtgga
    10621 atgtagtcta cgagtttcaa caaggtatcc attgaaaggg cacccaagca acccaaggta
    10681 aatccaataa aggcaaacaa ccaaattgtt gcatttagag tgaacttgaa tttcttcaaa
    10741 ttatggtttt tcaagtgctt catcgccgtt tcaggcgcac cagcacgtac agtgtaggcg
    10801 aaagtttgta agaattgaat cgccacagca atcggagtag agagggcaag agccgcttct
    10861 ggactgactt taccagcact agtgatagcc atcaaggtac caaagatacc aggtccgatt
    10921 gggtttggtg gaacagtacc tccagcacca acaccgaatc ccatataagc caattcacca
    10981 atagctccca ttgcaagagc agtaggcaaa tcacctagaa taattccgac accaaatgac
    11041 agaacaatac atctattggt gtaaattcct aacagcattc cactaaaaca gaaagctgtc
    11101 cataatccaa ttaaaatcgc ttgaaataca ttaatcgaca taacgatctc ctcttttaaa
    11161 tatagtccat aatgttgact tgaacagctc catcatttcc tgttggagtt gttttcgtat
    11221 taaatgttac ttgatgagtt tggctcaatt ccttgagggc cgccttgtcc tcttcaccca
    11281 ggaagatgga gcgtgtcact tgctctttac cagtagcatt gtgaatgttc ccaatattga
    11341 tttctttgat agggacacca ccttctacca aggttaaagc gtccttcaca tcctttacaa
    11401 cgataaagat cgtttgagca ggattagcct tgtgaatgat atcaatcacc ttttgcaaag
    11461 ggaagaaacg catggcaact gagtctggca caactgtttt catcagagtt tgctgcatct
    11521 tgtctgtgct tacttcgtca ttggcaacaa tgaccgtatt acaacctagg tattttaccc
    11581 aaagttgtcc ttgcccatga atcaaccgtt catcgatacg ggtcataata atatttggca
    11641 ttgtcatatt tctcctccta ccaatatagg ttccagtctt tgtagaaacg gataagggct
    11701 tctaggtaat agtagtcacc ccagatattg ccttcatcca ctcctttacc tgaatgccat
    11761 gagtacacac cgtggaggag acttgtccca ccaggggtaa attgatcatt tgcataatgt
    11821 tcgatcaagg aacgaagcat ggcatgcata gcatgtttat aaatatcttt gtcagcatcc
    11881 acctctggga gatgttttag catttcatga atcccacaga cggcgatagc tgttgctgaa
    11941 gaatctcgtg attgagcact accatcatta aaaatcaaat cccaatagga cacatgatct
    12001 tttggcagac gattcaagaa ataattggtc acacccttaa acaagtcaaa gcaggactcg
    12061 tcttttaagt gacgataagt caaaggaata ccatagactc cccatgattg accacgtgcc
    12121 cagcatgaat catcactata cccttgtctc gttacacctt taaagggttg acctgtctca
    12181 ggatcaaaat agaaggtgtg gaaggacgaa gcgtcatcac ggattacatt attagctgaa
    12241 gcatagaaat gactttctgc aatatcgtag tatttttgat cgcctgtttc ttgataagca
    12301 aagaataaga gttggatatt gagcaagcag tcgataatca aacggtaatg ctctttcttg
    12361 cccaagtctc cccaagcttg aataaaacca cctttttctt gatagcgttc aatcaactta
    12421 tctgcagctt tcaaggttgc ttctctagcc tctccatctc catttatctt atattcagcc
    12481 atacaagacg gtgtgtacaa gaagccgaga tcatggtgat ccaattctac tctcttattg
    12541 acacgatcca ggaaagaaag aacattttta tgagcgatgt ttttaaatgc atcctgttga
    12601 ctgtattcat aagccaacca cagttctcct gtccagaaac cattggtcca ttccgtgtta
    12661 tccatgattg gatagacatt atcaaaggta gctggcgtcg ggaaatcttc cttgaaatag
    12721 tcaaggttga gttctaactg ccgaataacc ttatcgattg cctggccgac ttcttctttc
    12781 gtcagaagtg gtacttctaa gaagcgctca ggcgatttta ttttttcaat cgtaaccttt
    12841 tttatcattc ttttaccctt taaatacccg attcgaaatc ttcttcctct tcctctgcat
    12901 ccgttgaaaa caattcttta ccatttacga ctccgccctg ggccgccact actgatttat
    12961 taacaacctc atcaaagcta tgagccattc tagcaaagac tgcttccatt aacatggcta
    13021 agttcaaacc cgagagaaca ttcattgttt tggctggatt ttctcccatt atggtagaag
    13081 aaaccttgaa tggcgatcct cccaagagat cacttaggat taaaacttct tcttcatttg
    13141 aaattgcaag taagattttt tgcttgagtt catctgctga cattccttcc acaaagtcaa
    13201 ttgcctccac attttcttga ttgcctgcaa tcaattgtaa agaactataa atccctgtag
    13261 caaaatgtcc atgccctaca agtacaattt tcatcttttc tcctttcttg tttaggttta
    13321 ccggtttact ttatattccc cattgtaatc ctttattttt atcttgtcaa tagttttttt
    13381 aaaaaaatat tagcgctttc attatctttt taatcatttt aatagacaga aatttcaaaa
    13441 tattccattt ttaaggaaaa taaatctttt tataaataaa aatatttatt tattgacaaa
    13501 accggtttac tagaatataa tttggttata gagaagtctt aacaaatttg aaaggaagta
    13561 tcaaatgaca aatacatcat tctcaattga gcagttttct ttaaaaggaa aaattgctct
    13621 catcaccggc gcttcttatg gaattggatt tgctattgcc aaatcctacg ctgaagccgg
    13681 cgctactatt gtctttaacg atatcaatca agatctggtc aataaaggga ttgaagctta
    13741 tcgtgaagtt ggcatcgaag cccatggata tgtctgtgac gtgacagacg aggacggtat
    13801 ccaagccatg gtcaagcaaa tcgaacaaga ggttggtgtc attgacatcc tcgttaataa
    13861 cgctggtatt atccgccgag ttccaatgtg cgaaatgagc gccgctgatt tccgtaaggt
    13921 catcgatatt gacttaaacg caccatttat cgtttcaaag gcagttattc cttctatgat
    13981 aaagaaaggg catggaaaga ttatcaatat ttgttcgatg atgagcgaac tgggacgtga
    14041 aacagttagc gcttatgctg ctgctaaagg gggcttgaaa atgttgaccc gcaacattgc
    14101 gtctgaatac ggtggagcca atatccaatg taacggaatt ggaccgggtt atattgccac
    14161 tcctcaaaca gcacctcttc gtgagttgca agaagatggt tctcgccacc catttgacca
    14221 gttcatcatt gcaaaaacac ctgctgcacg ttggggaaat cctgaagatt tgatgggccc
    14281 tgctgtcttt ctcgctagtg atgccagcaa ttttgtcaat ggccacatcc tatatgtaga
    14341 tggcggtatc ttagcctaca tcggaaaaca acctgagtaa aaatagaaag aagatcttat
    14401 gaaaatcgca ttaatcaatg aaaatagtca agctagcaag aatcacatta tttacgatag
    14461 tctaaaagaa gcgacagata aaaaaggcta ccaattattt aactatggta tgcgtggaga
    14521 agaaggagaa agtcaattaa cttatgtgca gaacggacta atggctgcca tccttttaaa
    14581 tacaaaggca gttgactttg ttgttaccgg ctgtggtacg ggtgtagggg ctatgcttgc
    14641 tttaaacagc ttccctggtg ttgtctgtgg tctagcagtg gacccaactg acgcttacct
    14701 ttattctcaa atcaatggtg gtaacgcctt gtctatccct tatgccaaag gatttggctg
    14761 gggggcagaa ctgaccctca aattgatgtt tgaacgctta tttgctgaag aaatgggcgg
    14821 tggctaccca agagaacgtg taatccctga acaacgcaac gctcgtatct taaacgaggt
    14881 gaaacaaatc acccacaatg atttgatgac catccttaaa acaatcgacc aagacttcct
    14941 caaagacacc atctctggca aatatttcca agaatacttc tttgaaaact gccaagatga
    15001 tgaagtcgct gcttatttga aagaagtatt agccaagtaa agctattcta aaccagaaag
    15061 gaactaatgg atgacgaaaa tattactgtt tggcgaacca ttaattcgaa tctcaccatt
    15121 agatgccacc agtatcggcg atcatgttgc cagttcgact tattttggcg gatcagaaat
    15181 taacatcgct tgtaatttgc aagccctggg tatctcaacg aaagtcttta ccgcactccc
    15241 tgccaacgag attggagatc gttttctcac attcttgaaa cagcaccaaa tcgataccag
    15301 ttcaatctgt cggcttggcg atcgaatcgg cctctactat ttggagaacg gctttggttg
    15361 tcgtcaaagt gaagttttct acgatcgtaa gcatacgagt atcagccaga ttcggccaaa
    15421 catgctagat atggattctc tctttcaggg gattagccat tttcatttta gtggaatcac
    15481 cgtagctatc ggtcaagagg tccgtgcgat ccttctccta ctcttggaag aagccaagcg
    15541 ccgaggaatt gtcgtttcaa tggatctcaa tctgagaaca aagatgattt cagtcctaga
    15601 agccaagtat gaattttcta agtttgcacg ttttactgac tattgcttcg gtattgatcc
    15661 tctcatgatt gatgaccaaa atctagagat gtttccaaga gacagtgcta gcctagaaga
    15721 ggtggaaaat cgcatgcgac ttttaaaaga agcctatggt ttcaaggcca ttttccatac
    15781 cctccgctct agtgatgagc aagacaaaaa tgtctatcaa gcctatgctc tggaagaacg
    15841 atttgaagag tctgtccaac taaaaactgc agtctatcaa cgaattggta gcggggatgc
    15901 ctttatatct ggtgcccttt accaactact ccatcattcc tccctaaaaa ctaccattga
    15961 ctttgcagtt gcgagcgcaa ctctcaaatg cactcttcca ggagaccatc tctccacttc
    16021 cgcaactagt attgaaaatt tactggcaaa tgcacaagat atcattcgtt aggagaatta
    16081 catgaccaaa tcagatacga ttattgaact aaaaaaacag aaaattgtcg ctgtcattcg
    16141 aggaaataca aaggaagaag gactgcaagc ctcgattgct tgtatcaagg gcggtatcaa
    16201 agctattgaa atcgcctata ccaatcagta tgcaggacaa atcatcaagg aacttgtaga
    16261 cttgtatcag gacgatcaga gtgtttgtat cggtgcaggt actgtgcttg atgccgtaac
    16321 tgctagagat gccattctag ccggagcaaa ttacgttgtt tctccatctt tccatgctga
    16381 aactgcgaaa atgtgcaatc tctacagcac accgtacatt ccaggctgta ttaccctcac
    16441 agagatcacg actgcacttg aagccggtag tgaaatcatc aaactcttcc caggtagtgc
    16501 tctcagtcca gcatatatct ctgcagtcaa ggcaccgatc ccacaagttt ccgtaatggt
    16561 aaccggagga gtcggtctaa acaacatccc tcaatggttc gctgctggtg cagacgccgt
    16621 tggaattggt ggcgaactca ataaactcgc ttcccaaggc aactttgacc gcatcagcga
    16681 gattgcccaa cagtatgtta cactcagata aaatcataac cacccgtcaa acgggtggtt
    16741 tatcccagag ctataagccc aaattaccag ccagcgccta aagacgctgg ctttcacgtt
    16801 gttcaagcct tattgctctt gactcgtcac ttgcctcttt aagagacttt ggtattactt
    16861 accactatcc ctaaagggat cctcatattc ttttacactc aatttatcta gtgctatagt
    16921 agattgaaac t
//
LOCUS       DAVBCW010000037        16775 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVBCW010000037 DAVBCW010000000
VERSION     DAVBCW010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16775
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.037"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            63..527
                     /locus_tag="VQ514_001043"
     CDS             63..527
                     /locus_tag="VQ514_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HET2499811.1"
                     /translation="MGGHPGFNCLLLDDEVYEDYYLEFEKEEICSVPRPFPETGMLDF
                     QDRSPWLEGQKEIDLSYDLFSTDAVTLDELQSRTIALRSLKHDKGLKVHFAEFPNLII
                     WSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            685..1110
                     /gene="lacA"
                     /locus_tag="VQ514_001044"
     CDS             685..1110
                     /gene="lacA"
                     /locus_tag="VQ514_001044"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HET2499812.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            1141..1656
                     /gene="lacB"
                     /locus_tag="VQ514_001045"
     CDS             1141..1656
                     /gene="lacB"
                     /locus_tag="VQ514_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HET2499813.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPSEENKKLIAKIEHLESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            1667..2596
                     /locus_tag="VQ514_001046"
     CDS             1667..2596
                     /locus_tag="VQ514_001046"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HET2499814.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDDQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKLVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLEELKEVLQESLFTGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAGLLIKA
                     NVLGMLNAQEKMTGHVNMANYQALYDQLIVKEV"
     gene            2598..3578
                     /gene="lacD"
                     /locus_tag="VQ514_001047"
     CDS             2598..3578
                     /gene="lacD"
                     /locus_tag="VQ514_001047"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HET2499815.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKILVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            3812..4648
                     /locus_tag="VQ514_001048"
     CDS             3812..4648
                     /locus_tag="VQ514_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HET2499816.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENH
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALIQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            4684..5001
                     /locus_tag="VQ514_001049"
     CDS             4684..5001
                     /locus_tag="VQ514_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2499817.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            5003..6697
                     /locus_tag="VQ514_001050"
     CDS             5003..6697
                     /locus_tag="VQ514_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET2499818.1"
                     /translation="MDKLIIFIEKGKPFFEKLSRNIYLRAIKDGFISSMPAVLFSSIF
                     ILIAAVPNIFGFKWSDEQLAFILKPYNYSMGILALLVAGTTAKSLTDSVNTRSMEKTN
                     QINYMSTFLAAVVGLLILAADPIEGGFANGLLGTRGLLTAFLAAFITVNIYKVCIKNN
                     VTIRLPEEVPPNIAQVFKDVIPFALSVLSIYGLDLIVRNIFGTNVAESVGKILAPLFS
                     ATDGYIGLAIVFGAYAFFWFVGIHGPSVVEPLIVAISYANIEANVQLVQAGMHADKIL
                     NPVTQTFVVTMGGTGATLVVPFMFMWLCKSKRNRIVGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFIPFVTAPIINVWIMKFFVDVLQMNSFSIILPWTTPAPIGIVMGTALAPLSF
                     VLAITLIIIDTLIYYPFVKVYDHQILEEERSGKSNDELKEKVAANFNTAKADAILEKA
                     GVDAAQNTITEETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLP
                     EFDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQAQFD"
     gene            6906..8312
                     /gene="lacG"
                     /locus_tag="VQ514_001051"
     CDS             6906..8312
                     /gene="lacG"
                     /locus_tag="VQ514_001051"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HET2499819.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPLDPKNPSDVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     VAPDYVPRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            8355..8537
                     /locus_tag="VQ514_001052"
     CDS             8355..8537
                     /locus_tag="VQ514_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HET2499820.1"
                     /translation="MFKDFIQSIYEKVYIINFEKCSQIPCLTSEELKSLFLNFINPEE
                     WDTISFDSDFMPFQQS"
     gene            complement(8815..9576)
                     /locus_tag="VQ514_001053"
     CDS             complement(8815..9576)
                     /locus_tag="VQ514_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET2499821.1"
                     /translation="MLKQEKLAKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDF
                     YTFYNVSNLDTIVSDSKLSDSILFKLSKHIKVIKP"
     gene            complement(9758..10720)
                     /gene="nrdF"
                     /locus_tag="VQ514_001054"
     CDS             complement(9758..10720)
                     /gene="nrdF"
                     /locus_tag="VQ514_001054"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HET2499822.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            complement(10908..13067)
                     /gene="nrdE"
                     /locus_tag="VQ514_001055"
     CDS             complement(10908..13067)
                     /gene="nrdE"
                     /locus_tag="VQ514_001055"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HET2499823.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            complement(13148..13366)
                     /gene="nrdH"
                     /locus_tag="VQ514_001056"
     CDS             complement(13148..13366)
                     /gene="nrdH"
                     /locus_tag="VQ514_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HET2499824.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
     gene            13717..13980
                     /locus_tag="VQ514_001057"
     CDS             13717..13980
                     /locus_tag="VQ514_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HET2499825.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            13986..15719
                     /gene="ptsP"
                     /locus_tag="VQ514_001058"
     CDS             13986..15719
                     /gene="ptsP"
                     /locus_tag="VQ514_001058"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HET2499826.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            15974..>16775
                     /gene="phtA"
                     /locus_tag="VQ514_001059"
     CDS             15974..>16775
                     /gene="phtA"
                     /locus_tag="VQ514_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HET2499827.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWVPSVSNPGTT"
BASE COUNT         5263 a         2950 c         3487 g         5075 t
ORIGIN      
        1 aactagaatt ctagttttgc aggaagtcta ataaagaaac tagcaaggtc aggccttact
       61 ttattggtgg acatccagga tttaattgtc ttctacttga tgatgaagtc tatgaagatt
      121 attatctaga gtttgagaaa gaagagattt gctctgttcc acgtcctttc ccagaaacag
      181 gtatgttgga tttccaagat agaagtccat ggctagaggg acaaaaagaa atagatctta
      241 gttatgatct tttcagcaca gatgcagtga ctttggatga attgcaatct cgaacaattg
      301 cccttcgttc tcttaaacat gataagggat tgaaagtgca ttttgcagaa tttccaaacc
      361 tcatcatctg gtcaactttg aacaagggac ctttcattac ctttgaacca tggtctggct
      421 tgtcaacatt ccttgaagaa ggagatcatt tagaagataa gaagaatgtt tgtctcttag
      481 aagccaatca ggttgaagaa ttagggtttg agatagaagt tttataaaaa aagtaaaaaa
      541 taaataacaa aaacaaacac aaactgttga cttcttcaaa caatagtagt atattatgtt
      601 tgtgaggact aattgactgt tttttaaaga cagccaatac actgctggaa tccagcatag
      661 aaaaaataaa aaggagtata caatatgtct attgttatcg gtgcagatgc tgcaggtttg
      721 agattgaaag aagttgtgaa agacttctta gaaaaagaaa acttccacct tgtggatgtt
      781 acagctgaag gtcaagactt tgttgatgtg actctcgctg ttgctgcaga agtaaacaaa
      841 gaagaacaaa accttggtat cgtgattgat gcttatggag ctggtccatt tatggttgca
      901 actaagatca aaggaatggt tgctgcagaa gtatctgacg aacgttcagc ttatatgact
      961 cgtggccaca acaactcacg tatgatcact atgggggcac aacttgttgg tgatgaattg
     1021 gctaaaaaca tcgctaaagg atttgttaat ggtaaatacg acggtggacg tcatcaaatc
     1081 cgggttgaca tgttgaacaa aatgggctaa ttagattaca gtaagaaagg taagttaaaa
     1141 atgagaattg caattggatg cgaccacatc gtaacagatg aaaaaatggc ggtttcagaa
     1201 tttttgaaat caaaaggata tgaagtcatt gactttggta catatgacca tacacggact
     1261 cactacccaa tctttggtaa aaaagtaggg gaagctgtaa ctagcggcca agctgatctt
     1321 ggggtatgta tctgtggtac tggtgttggt atcaacaacg ctgtaaataa agttccaggt
     1381 gttcgttctg ccttggttcg tgatatgaca acagcccttt atgctaaaga acaattgaac
     1441 gctaacgtta tcggctttgg tggtaagatt actggtgaat tgcttatgtg tgatatcatc
     1501 gaagctttca ttcatgctga atacaaacca tctgaagaaa acaagaaatt gattgcgaaa
     1561 attgaacacc ttgaaagtca caatgctcaa caaacagacg caaacttctt tacagaattc
     1621 cttgaaaaat gggatcgcgg agaataccac gactaagagg tgacctatga ttttaacagt
     1681 cacaatgaac ccatccattg atatttccta tcccttggat gagttgaaga ttgatactgt
     1741 caatcgtgtg gtggatgtaa ccaaaacggc tggtggtaag ggacttaatg ttacccgagt
     1801 actttcagaa tttggtgatt ctgttcttgc tactggttta gtgggtggca aacttggtga
     1861 gtttttggtt gaacatatcg atgatcaagt aaagaaagat ttcttttcta ttcagggaga
     1921 aactcgtaac tgtatcgcta ttctccacgg tgacaaccaa acagaagttc ttgaaaaagg
     1981 tcctgaagta ttggaacagg aaggtcaaga ctttttggaa catttcaaaa aactcttgga
     2041 gtcagttgaa gtagtagcca tctcaggtag tctgccagct ggccttccag ttgattacta
     2101 tgcgagcttg gtagagcttg ctaatcaagc tggcaagcta gtagtcttgg actgctctgg
     2161 tgcagcactt caggcggttc ttgaatcacc ccataaaccg acagtaatca aacccaataa
     2221 tgaggaattg tctcagcttc taggaagaga agtttctgag gatttggagg aattaaaaga
     2281 agtccttcaa gaatctttgt ttacagggat tgaatggatt atcgtttcac ttggtgccaa
     2341 cggtactttt gccaaacatg gtgacacttt ctacaaggta gatattccta gaattcaggt
     2401 ggtaaatcct gttggatctg gagactctac tgtggcagga atttcttcag gacttcttca
     2461 caaagaatcg gatgcaggat tactcatcaa ggcaaatgtc cttggtatgc tcaatgctca
     2521 agaaaaaatg actggtcatg tcaacatggc caactatcaa gctctatatg atcaattaat
     2581 agtaaaagag gtataaaatg gctttaacag aacaaaaacg tgtacgctta gaaaaacttt
     2641 ctgatgaaaa tggtatcatc tcagctcttg catttgacca acgtggtgct ttgaaacgcc
     2701 tcatggctca acaccaaaca gaagaaccaa ctgtggccca aatggaagaa cttaaaatct
     2761 tggtagcaga tgaattgact aaatacgctt catctatgct tcttgaccct gagtatggac
     2821 ttccagcaac taaggctctt gatgaaaaag ctggtcttct ccttgcttat gaaaaaacag
     2881 gttatgacac aacaagcaca aaacgcttgc cagactgctt ggatgtttgg tctgcaaaac
     2941 gtattaaaga agaaggtgca gatgcagtta aattcttgct ttactatgat gtagatagct
     3001 cagacgaact caatcaagaa aaacaagcct acatcgaacg catcggttct gagtgtgtgg
     3061 ctgaagatat cccattcttc cttgaaatcc ttgcttacga tgaaaaaatt gcggacgcag
     3121 gttctgtaga atacgctaaa gtaaaaccac acaaagttat cggtgctatg aaagtctttt
     3181 cagacccacg ttttaacatt gatgtcttga aagttgaagt tcctgttaac attaaatatg
     3241 ttgaaggctt cgctgagggt gaagtggttt acacacgtga agaagcagca gccttcttca
     3301 aagcgcaaga tgaagcaacg aacttgccat acatctactt gagtgctggt gtatcagcta
     3361 aactcttcca agatactctt gtatttgctc atgaatcagg tgcaaacttt aacggagttc
     3421 tttgtggtcg tgctacatgg gcagggtcag ttgaagctta catcaaagat ggtgaagcag
     3481 cagctcgcga atggcttcgc acaactggat ttgaaaacat tgatgagctc aataaagttc
     3541 ttcaaacaac agcgacttca tggaaagaac gtgtgtaaga aagtcctcct agtttaggaa
     3601 catgaatcta aaaaaattta aaaaaagttg tatgtaaagg tttacaaaat aacttacttg
     3661 tgctatactt aaatcacaag ttaatacaag gtgagtgtta ctaagtaata ttaggcatga
     3721 tcacaggtga attagaaatc agctgatttt ctagttcatt tgtggtcatt ttttgtactc
     3781 atataccttt aagatataaa aggaggttga catgtatcga attctaaatc caatgaatca
     3841 caatgtctcg cttgtcagaa atgataaggg agaagaggtg attgtaattg gtaaggggat
     3901 tgcattcgga aagaagaagg gggatttgat tgctgaaaat catgttgaga aaatctttcg
     3961 gatgaagacc gaagagtcca gagaaaactt tatggctctt ctcaaagatg ttccgcttga
     4021 ttttatcaca gtgacctatg aaatcattga taagctatca aagaaatatc attatccgat
     4081 tcaagagtat ctctatgtaa ccttgacaga tcatatttac tgttcttatc aagctctaat
     4141 tcaaggaagg tacaaggata gtaatctgcc agatatttct gctaagtatc ctgtcgcttt
     4201 tcaaatcgca aatgaagctt ttgaaattta ccgtcagaag ctagcagatc attttcctga
     4261 ggacgaaatt attcggattg cttatcattt cattaatgct gaaggtgaaa atgaagtgga
     4321 acttgtggag tcgattgata agaggaaaga aattctcagg aatgttgaag aagttttaac
     4381 ggactatgca attcaacgaa ctaaaaagaa taaccatttc tatgatcgct ttatgatcca
     4441 tttgaattat ttcttggatt atttagacag atctagagat gataaccaat cacttctgga
     4501 tatggaagat catattaaac aatcctatcc aaaagccttc gagattggtt ccaagatcta
     4561 tgatgtgatt acgcaacata cgggtcttga tttgtataaa agtgaacgag tttatctagt
     4621 tctacatatc caacgtttat tgtcataaaa atttatttaa aactatataa ggagaattct
     4681 atcatgaata gagaagaagt aacattgtta ggttttgaaa tcgtagccta tgctggcgat
     4741 gctcgttcaa aactattgga agccttgaag gctgctgaag ctggtgattt tgaaaaagcg
     4801 gacgctctgg tagaggaagc tggtagctgt attgcagagg ctcaccacgc gcaaacaagt
     4861 ctattgacta aggaagcttc tggtgaggac ttagcgtata gtgtgaccat gatgcatggt
     4921 caagatcact tgatgacaac tatcttgtta aaagacctaa tgcaccattt aattgaactt
     4981 tacaagagag gagttaaata aaatggataa gttaataatt tttattgaga aaggaaagcc
     5041 tttctttgag aaattatcga gaaatatata cttaagagcg ataaaagatg gttttatttc
     5101 cagtatgcca gcagtattat tctcaagtat ttttatacta atagctgctg tgcccaatat
     5161 ttttgggttt aaatggtctg atgaacagtt agcttttatt ttgaaacctt acaactactc
     5221 aatggggatt ttagctctct tagtagctgg gacaactgca aaatcgttaa cagattcagt
     5281 taatacacgt agtatggaga aaaccaacca aatcaactac atgtcaactt ttttagcagc
     5341 tgttgttgga ttgttaatac tggcagctga tccaattgaa ggtggatttg ctaatggtct
     5401 tttaggtact agaggattac ttactgcatt tttggcagct tttattacag taaatatcta
     5461 taaagtatgt attaaaaata atgtaaccat tagacttcct gaagaggttc caccaaatat
     5521 tgcacaagtt tttaaagatg ttattccgtt tgccttatct gttctttcaa tatatggatt
     5581 ggatttaatt gttagaaata tttttggtac aaatgtagca gaatctgttg gtaaaatatt
     5641 agcccctcta ttttcagcta ctgatggtta tatcggttta gctattgtat ttggtgctta
     5701 tgctttcttt tggtttgttg gaattcatgg gccatctgtt gtagaacctt taattgtagc
     5761 tatcagttat gcaaatatag aagctaatgt tcagcttgtg caagctggta tgcacgcaga
     5821 taaaatttta aatccagtta ctcaaacttt tgttgtaaca atgggtggta ctggtgcaac
     5881 actagtagtt ccatttatgt ttatgtggtt atgtaaatca aaaagaaata gaattgtcgg
     5941 acgtgcgtct gtagttccta ctttctttgg cgtaaatgaa ccaatcctat ttggtgctcc
     6001 aattgtttta aatcctatct tctttattcc gtttgttact gctcctatta tcaatgtttg
     6061 gattatgaaa ttttttgtag atgttctaca aatgaatagt ttcagtatta ttttaccttg
     6121 gacaacacct gctccaattg gtattgttat gggaacggcg ctggctccat tgtcattcgt
     6181 cttggctata actttaatca ttatcgacac tttaatctat tatccatttg ttaaagttta
     6241 tgaccatcaa attttagaag aagaacgttc aggtaagtct aatgatgaat tgaaagaaaa
     6301 agttgctgca aacttcaaca ctgcaaaagc ggatgctatt cttgaaaaag cgggtgtcga
     6361 tgcagcacaa aataccatca ctgaagaaac aaatgtcctc gttctctgtg caggtggagg
     6421 aacaagtggt ctccttgcaa atgctttgaa taaggcagca gcagaataca atgtccctgt
     6481 gaaagcagca gcaggcggct atggtgctca ccgtgaaatg ttaccagagt ttgatcttgt
     6541 tatccttgcc cctcaagttg cttcaaactt tgaagatatg aaagcagaaa cagataagct
     6601 cggtattaaa ctagcgaaaa cagaaggcgc tcaatacatc aaattaactc gtgatggaaa
     6661 aggtgctctt gcattcgtac aagcgcaatt cgattaaggc tagagactct gaaatagtct
     6721 cctatcgtta cggaaatcgc tatggcgaat ttcctattat taattcgtcg gtaaaaagat
     6781 atcgttttta cctcctcatg tcacaattcg gtgacttggt acaagaagtg agatggagaa
     6841 ggatggctca ctgactcctc tcctctcact tttactttat ttaaatcaag aaataggtga
     6901 aaaaaatgac aaaaacactt ccaaaagact ttatttttgg tggcgcaaca gctgcctatc
     6961 aagcagaagg tgctacacat actgatggta aaggaccagt agcgtgggat aaatatcttg
     7021 aagataacta ctggtacact gcagaaccag ccagtgattt ttacaatcga tatccagttg
     7081 acctcaagct agcagaagag tatggtgtca atggtattcg aatttctatt gcttggtcac
     7141 gtattttccc gactggttac ggccaagtaa atgctaaagg tgttgagttt tatcataatt
     7201 tatttgcaga gtgtcacaaa cgtcatgttg agccctttgt aactcttcat cactttgaca
     7261 cgccagaagc tctccactca aatggagact tcttaaaccg tgaaaatatc gaacattttg
     7321 tagactacgc tgccttctgt tttgaagaat ttccagaagt aaactattgg acaaccttta
     7381 atgaaattgg gccaattggt gatggtcaat acttggttgg gaaattccct ccaggtattc
     7441 agtacgacct tgccaaagtc ttccaatcgc accacaatat gatggtgtct catgcgcgtg
     7501 cagttaagct atataaagat aaaggctata aaggcgaaat tggtgttgtt cacgctctgc
     7561 caactaagta tcctttagat cctaaaaatc cgtcagatgt tcgtgcagct gagttggaag
     7621 atattattca caataaattt atcttggatg caacttatct aggtcgctat tcagctgaaa
     7681 cgatggaagg tgtcaaccat atcttatcag tcaatggtgg tagcttggat cttcgtgaag
     7741 aagattttac agcattagaa gcagcaaaag acttgaatga tttccttgga atcaactact
     7801 atatgagtga ctggatggaa gcctttgatg gagaaactga aattatccat aatggtaagg
     7861 gtgagaaagg aagttctaaa taccaaatca aaggtgttgg tcgtcgtgta gctcctgatt
     7921 atgtaccacg tacggattgg gattggatta tctaccctca aggtttatat gaccaaatta
     7981 tgcgtgtgaa gaaagattat cctaactaca agaagattta catcactgaa aatggtctcg
     8041 gctataaaga tgagttcgtc gataacactg tttatgatga tggacgtatt gattatgtga
     8101 aacaacactt ggaggtcttg tctgatgcga ttgcagatgg agcgaatgta aaaggttact
     8161 tcatttggtc attgatggac gtcttttcat ggtcaaatgg ttatgaaaaa cgttacggtc
     8221 tcttctatgt agactttgaa actcaagaac gttatccgaa gaaatcagct cactggtaca
     8281 agaaagtagc ggaaactcag attatagact agtagaatta gtcattagat atagaatttt
     8341 agtgagtcaa aaagatgttc aaagatttta tccaatctat ttatgaaaaa gtttatatta
     8401 taaatttcga aaaatgctct caaataccgt gtttgacgag tgaagaattg aaaagtctat
     8461 ttttaaattt tatcaatcct gaagaatggg atactatctc ctttgattca gattttatgc
     8521 catttcaaca atcgtaacca atttctcaaa aaagttaaat cttatattta gtactctgca
     8581 aaactcttat ctaatcacgt tgcttatact caatgaaaat caaagagcaa ctttaaacta
     8641 ggaagcgagt cgcagatttc tcaatgcata gctttgagga attgggcaaa aagtctttga
     8701 tatagaaaaa gcatagtatc aggtgtttca acacctgata ctatgctttt tattgtggga
     8761 agatttactt tttttcttct gaaattgagt tgttacccag gctctttcag tttattaagg
     8821 cttgatgact ttaatgtgtt tagatagctt aaaaaggatt gaatcactta gtttagaatc
     8881 tgaaacaata gtatcaagat ttgatacatt ataaaaagta taaaaatcaa acttattgaa
     8941 cttactatga tctgcgagta aatatttttt attagaatta tttaaagcga tgcgttgagc
     9001 ctctccctct tcctcgctaa aagtagctag agctccgttt tgaataccat tacagctaac
     9061 gaaagcttta gaaaattgga gattagagag attttgtagg gtcaatgtgc caacaaaagc
     9121 acctgtaata tcgcgataat ttccacctat caaaatcaaa tctgttaatt ttcgttcgct
     9181 taaaatcaga aaaacaggta gactgttggt tacgacgcgg atattgtcaa taggcaactc
     9241 acgcgcaaaa aactctaatg ttgttcctgg tccaatgaaa atagtttctc tttcttctac
     9301 tagactgcct gcaaaatggg ctatttcttg tttttctgcc gtttggaggg cttgtttttc
     9361 aatatttgat cgctcattag tcaaaaggga gttggttcga agtttttcag ctccaccatg
     9421 cacacgaatc agcaaatctt tatcagctaa ttcctgtaaa tagcgccttg cagtcatatc
     9481 tgaaacggct atttcgtcca taatctgttt aactgttatg gttcctttac tatttactat
     9541 ctctaaaatt ttggctaatt tttcttgttt gagcatatta tcacctcgtt tcctactact
     9601 atcttaccat aaacaaactc atcattcaaa tacaaaaaca acaaaatgaa acaaaaacaa
     9661 aaatatcgaa gtttgttttc aaaactttcg atatttttgt tgggttataa ctttgatgtt
     9721 tctagtttac tttttgatga ttgagagtga tggagaatta gtctaaaccg tagttatagt
     9781 catcgtcttg catggcttca acttcgccaa gaaggtaacc atttccgact tgagagaaga
     9841 agtcatggtt ggaagttcct gttgaaatac cgttcataac gattgggttg acatcttcag
     9901 ctgaatctgg gaaaagtgga tcttgtccca tgttcatgag agctttattg gcattgtagc
     9961 gaaggaaggt tttaacctct tcagtccaac caacaccgtc ataaagactc tctgtgtagc
    10021 cttcttcatt ttcataaaga gtatagagta ggtcgtacat ccattctttg agtttttctt
    10081 gctcttcttc aggcaattca ttgaaaccaa gttggaattt gtaaccaatg taggttccgt
    10141 gaacagactc gtcacgaata atcaatttaa tgatttctgc aacgttggct agtttgttgt
    10201 taccgagata gtagagggga gtgaagaaac cagagtagaa gaggaaggtt tcgaggaaga
    10261 cgctggcaac tttcttttca agtgggctgc cgtttaggta gatttcgttg acaatctcag
    10321 ccttcttttg taggtaagga ttggtattgg tccattcgaa aatttcttca atctcagcct
    10381 tagtattcaa ggtagaaaag attgatgagt aagatttagc gtggacagat tccataaatt
    10441 ggatgttatt gaaaacagct tcctcatgtg gtgtacggat gtctgcgcga agggcttgaa
    10501 ccccagtttc agattgcata gtgtcaagaa gggttaaacc accaaaaact tttccgacca
    10561 agtctttctc tttgttagat agctttctcc agtcatccaa gtcgtttgat aagggaatac
    10621 gtgtatcgag ccaaaattgc tccgtcagtt tttcccaagt tgatttgtcg atgacatctt
    10681 cgatggcatt ccagttaatg gctttgtagt aagtttccat ttaaaatctc tttctgtgtt
    10741 tagtattgcg aactcacaat tatttctact ttaccataat tctataggag tatcgcacaa
    10801 aaagtcggaa gcccgacttt taaaatgtta cataaattat gttatgacat agtagatttg
    10861 attttatcag tgctgcttag ggaaaaataa tgtttctatg ctagaaacta aatcacacag
    10921 ctttcacatt ggttggcgcc gacttctcca ccgtcatctg taaaggtacg gacgtagtag
    10981 atagacttga ttcccttgtt aaaggcatag ttacgaagga tggacaagtc acgtgtcgtt
    11041 tgtttatttt ccctcttcca ttcgtaaagg ccttttggaa tgtcactacg catgaagagg
    11101 gtgagtgaaa gtccttgatc cacgtgttca gtcgcagcag cgtaaacatc gatgacttta
    11161 cgcatatcca tatcgtaggc agaagtgtag taaggaatgg tttctgtaga caagccagca
    11221 gcagggtagt agattttacc aattttcttc tcttggcgtt cttcgatacg ttgcgtaatc
    11281 gggtggatag aagcagaaac gtcgttgata tagctgatag aaccatttgg cgctacagca
    11341 aggcgatttt ggtggtaaag accatcttct tgaaccttgt cgcgaagttc agcccaatca
    11401 gcaacaccag ggataaagac atttttgaag agttctttaa cacggtctga tgttggaaca
    11461 aattcaccag ttacatactt gtcaaagtaa cttccgttag catagtctga tttttcaaag
    11521 ttgtggaagg taataccacg ttcacgcgca atattgtttg actctaccaa ggtccagtag
    11581 ttcataagca taaagtagat gcttgtaaat tcaacagact caggtgaacc atattcaatg
    11641 agttgttggg caaggtagct gtgcagtccc atggcaccga gaccaaaggt gtgggcttgg
    11701 ctatttccat ggtcaatcgt tggtacagct acgatatgtg aactatctgt aacgaaagta
    11761 agggcacgaa ccatagcacg gatagaacga ccaaaatcag gtgaagtcat catgttaacc
    11821 acgttggttg aacccaggtt acatgaaaca tctgttccca tttgaaggaa ttcttgagca
    11881 tcgttgatca agcttggttc ttgaacttga agaatctcag aacacaagtt actcatgata
    11941 atctttccat caacaggatt tgcacggtta gccgtatcga tgttgactac ataaggatag
    12001 ccagactctt gttgcaattt agagatttca gtttccaaat cccgcgcctt gatttttgtc
    12061 ttgcgaatat ttggatttgc gaccaattca tcgtattttt cagtaatgtc gatgtaattg
    12121 aatggcacac cgtattcttt ttctacagag taagggctga agaggtacat ttcttcattt
    12181 ttacgagcca attcgtagaa tttatcaggt actacaacac caagtgatag agtcttgaca
    12241 cgtacttttt catcagcgtt ttctttctta gttgaaagga aagcgatgat atctgggtga
    12301 aagacgttga ggtagacaac accggcacct tgacgttgcc ccaattggtt ggagtaagag
    12361 aagctgtctt caaaaagctt cataacagga acgacacctg aagcagctcc ttcatagcct
    12421 ttgataggtg caccagcttc acgaaggttg ctgagggtaa ttcccacacc accaccaata
    12481 cgtgaaagtt gaagagctga gttgatagaa cgtccgatag agttcatatc atccgtcact
    12541 tggattagga aacaagatac caactctcca cggcgagcac gtccagcatt caagaaggaa
    12601 ggagtagcag gttggtagcg ttggtggatg atttcattgg caatatcgat tgcaacagct
    12661 tcattcccat cagcgaaata aagggcgttg aagaagacac ggtcttccat attttcaaga
    12721 tagtattcac cgtcattagt ctttaaggca tattgattgt aaaatttata agctgccatg
    12781 aatgacttga attggaagtt ttggtctttg ataaattgag ctaattcttc caagaactct
    12841 ggacggtatt tcttgataaa ggctgtttcg atgtagttgt gttcaatgag gtaattgatt
    12901 ttgtctttga ttgaatcaaa aaccatagtg tttggaacta cattttcttt aaagaaagca
    12961 tccaaggctt ccttgtcttt atgaagcatg atttgtccat taacaggacg gttaatttcg
    13021 ttattaagac ggaagtaagt cacgtcttca agatgtttta atcccataaa atttccctta
    13081 tctaattaca aaagaaaggc ttctaagtta gccctaaaag cagtttcttc tggatgatgt
    13141 actaagatta tgctaattgt ttcagttttc ctggttggaa acctgaaaag acttcagttg
    13201 gtgtttggat aacaggagct gcgctaaaac cgagctcttt aacttgatcg acgtactcag
    13261 gttgctcatc aagattgatt tcacgataag agacattatt actgtccaag aaacgcttgg
    13321 tcattttaca ttggacacaa ttgtttttag aataaacggt taccattgtg taactcctct
    13381 tcaaaattta atactatctt agtatatcag aaaataaaat tttgtcaatg atttgaacta
    13441 aatatagtgg tgttcttttg ttgtttttat atataaacac aatatatagt gtttgtattg
    13501 tgaaatagca atgattatcc tataaattgt ttttaaagaa actatatcct gtgttttgtt
    13561 atctaggata gaagattttc agcaagttat ctgaaaggaa attgaaggaa aatccagaaa
    13621 atgcttgaaa aaaatcctag aagatggtat aatactaaat tgtaagggtt atcacatata
    13681 actcaaaaaa agaaagaaca aaaggagagt caaactatgg cttctaaaga tttccacgta
    13741 gtggcagaaa caggtattca cgcacgtcca gcaacattgt tggtacaaac tgctagcaaa
    13801 tttgcttcag atatcactct tgagtacaaa ggtaaatcag ttaaccttaa atcaattatg
    13861 ggtgttatga gtcttggtgt tggccaaggt gctgacgtaa ctatctcagc tgaaggtgca
    13921 gatgcagatg acgctatcgc tgcaatctca gaaacaatgg aaaaagaagg attggcataa
    13981 gggaaatgac agaaatgctt aaaggaatcg cagcatctga cggtgttgca gttgcaaaag
    14041 catatctact cgttcagccg gatttgtcat ttgagactat tacagtcgaa gatacaaacg
    14101 cagaagaagc tcgccttgat gccgctctac aggcatcaca agacgagctt tctgttattc
    14161 gcgagaaagc agtaggtacg ctcggtgaag aagcagctca agtttttgat gctcacttaa
    14221 tggttcttgc tgacccagaa atgatcagcc aaatcaagga aactatccgt gcgaagaaag
    14281 tgaatgcaga agcaggtctg aaagaagtta cagatatgtt tatcactatc tttgaaggca
    14341 tggaagacaa cccatacatg caagaacgcg cagcggatat ccgcgacgtg acaaaacgtg
    14401 tattggcaaa ccttcttggt aaaaaattgc caaacccagc ttctattaat gaagaagtga
    14461 ttgtgattgc gcatgacttg acaccatctg atacagctca attggacaaa aactttgtaa
    14521 aagcttttgt aaccaacatt ggtggacgta caagccactc agctatcatg gcacgtacac
    14581 ttgaaattgc tgctgtatta ggtacaaata acatcactga aatcgttaaa gacggtgaca
    14641 tccttgctgt taacgggatc actggagaag tgattatcaa cccaacagat gaacaagcgg
    14701 cagaatttaa agcagctggt gaagcctatg cgaaacaaaa agctgaatgg gcacttttga
    14761 aagatgctca aacagtgact gctgacggta aacacttcga gttggctgcc aatattggta
    14821 ctccaaaaga cgttgaaggt gttaacaaca acggtgcaga agctgttggg ctttaccgta
    14881 cagagttctt gtacatggat tctcaagact tcccaactga agatgagcag tatgaagcat
    14941 acaaggctgt tcttgaagga atgaacggta aacctgttgt cgttcgtaca atggatatcg
    15001 gtggagataa ggaacttcct tacttcgata tgcctcacga aatgaaccca ttccttggat
    15061 tccgtgctct tcgtatctct atctctgaga ctggagatgc tatgttccgc acacaaatcc
    15121 gtgctcttct tcgtgcgtct gttcacggtc aattgcgtat catgttccca atggttgcgc
    15181 tcttgaaaga attccgtgca gcgaaagcag tctttgatga agaaaaagca aaccttcttg
    15241 ctgaaggtgt tgcagttgcg gataacatcc aagttggtat catgatcgag attcctgcag
    15301 cggctatgct tgcagaccaa tttgctaaag aagttgactt cttctcaatt ggtacaaacg
    15361 acttgatcca atatacaatg gcagcagacc gtatgaacga acaagtttca tacctttacc
    15421 aaccatacaa cccatcaatc ctacgcttga ttaacaatgt gatcaaagca gctcacgctg
    15481 aaggtaaatg ggctggtatg tgtggtgaga tggctggtga ccaacaagct gttccacttc
    15541 ttgtcggaat gggcttggat gagttctcta tgtcagcaac atctgtactt cgtacacgca
    15601 gcttgatgaa gaaactcgac acagctaaga tggaagagta cgcaaaccgt gcccttacag
    15661 aatgctcaac aatggaagaa gttcttgaac ttcaaaaaga atacgttaat tttgattaat
    15721 cgaaaagtcc ctgcaactca gttacaggga ctttttttga tattttaaaa agaattttca
    15781 agaaaatctt tcttataaaa agtccaacct tgaaaaagta gtggtcagaa caaaaaatac
    15841 ttaaatggtt cataaaattc ttgacaagtt ggagatttag gagtaaacta ttaaccagtt
    15901 aagtaataga gaggagtttc tgcaatttag aaatgaattg caactagaaa tatcaaatag
    15961 aaagagagtt tcgatgaaaa ttaataagaa ataccttgtt ggttctgcgg cagctttgat
    16021 tttaagtgtt tgttcttacg agttgggact gtatcaagct agaacggtta aggaaaataa
    16081 tcgtgtttcc tatatagatg gaaaacaagc gacgcaaaaa acggagaatt tgactcctga
    16141 tgaggttagc aagcgtgaag gaatcaatgc tgagcaaatc gtcatcaaga taacagacca
    16201 aggctatgtc acttcacatg gcgaccacta tcattattac aatggtaagg ttccttatga
    16261 tgcgattttc agtgaagaat tactcatgaa agatccaaac tataagctaa aagatgagga
    16321 tattgttaat gaggtcaagg gtggatatgt tatcaaggta gatggaaaat actatgttta
    16381 ccttaaggat gctgcccacg cggataacgt ccgtacaaaa gaggaaatca atcgacaaaa
    16441 acaagaacat agtcaacatc gtgaaggtgg gactccaaga aacgatggtg ctgttgcctt
    16501 ggcacgttcg caaggacgct atactacaga tgatggttat atctttaatg cttctgatat
    16561 catagaggat actggtgatg cttatatcgt tcctcatgga gatcattacc attacattcc
    16621 taagaatgag ttatcagcta gcgagttggc tgctgcagaa gccttcctat ctggtcgggg
    16681 aaatctgtca aattcaagaa cctatcgccg acaaaatagc gataacactt caagaacaaa
    16741 ctgggtacct tctgtaagca atccaggaac tacaa
//
LOCUS       DAVBCW010000038        16132 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVBCW010000038 DAVBCW010000000
VERSION     DAVBCW010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16132
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.038"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            145..6831
                     /locus_tag="VQ514_001060"
     CDS             145..6831
                     /locus_tag="VQ514_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET2499828.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTVEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQTSNQEKPLKEDAKAVTNEEVNQMIEDRKVDFNQNWYF
                     KLNANSKEAIKPDVDVSTWKKLDLPYDWSIFNDFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGFKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SNQVTVFTGKKEGQEKTVLGTEVARVRTLIGKEPKMPKTVGFVYSDGSREKLPVTWSQ
                     VDVSQAGVVTVKGTANGREVEAHVEVLAIAKELPTVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDSWEITEVDKAKLSVAGSRIQMTGQLAGETIHATLVVEEGNAAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAENADVTVLQASAANGMRASIFIQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLKAEYEGAKGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQKPTLPTTVTVEYDKGFPKAHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRIEQYA
                     KEGVFTVNGRLEDTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRTNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAVRIRMVKADNKRGTSITEVQIFAKQVAAAKQGQIRIQVDGKDLANFNPD
                     LTDYYLESVDGKVPAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKDLTVEWEEVPAEN
                     LTKAGQFTVRGHVLGSDLVAEVTVRVTDKLGEALSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQAKLRFFA
                     DSGTDAPTKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IQLTKEKPVSAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGKERIFT
                     AINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSEASQTN
                     KAQLPNTGSAASQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(7079..7935)
                     /locus_tag="VQ514_001061"
                     /pseudo
     CDS             complement(7079..7935)
                     /locus_tag="VQ514_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8146..8505)
                     /locus_tag="VQ514_001062"
     CDS             complement(8146..8505)
                     /locus_tag="VQ514_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HET2499829.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSILAIILVFFFLLKKRSFYYPKFIKFFWRAGFL
                     LTLIMYIEMIVELFLMK"
     gene            8597..9553
                     /locus_tag="VQ514_001063"
                     /pseudo
     CDS             8597..9553
                     /locus_tag="VQ514_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            9537..10094
                     /locus_tag="VQ514_001064"
     CDS             9537..10094
                     /locus_tag="VQ514_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET2499830.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQADLTNAQLILQGHETLDQFVIKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            10087..10341
                     /locus_tag="VQ514_001065"
     CDS             10087..10341
                     /locus_tag="VQ514_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499831.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTHHERSVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            10353..12407
                     /locus_tag="VQ514_001066"
     CDS             10353..12407
                     /locus_tag="VQ514_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET2499832.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKDKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            12586..13446
                     /locus_tag="VQ514_001067"
     CDS             12586..13446
                     /locus_tag="VQ514_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET2499833.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLCFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNLSNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            13657..14367
                     /gene="ccdA2"
                     /locus_tag="VQ514_001068"
     CDS             13657..14367
                     /gene="ccdA2"
                     /locus_tag="VQ514_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HET2499834.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            14387..14953
                     /locus_tag="VQ514_001069"
     CDS             14387..14953
                     /locus_tag="VQ514_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HET2499835.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            14964..16076
                     /gene="msrB"
                     /locus_tag="VQ514_001070"
     CDS             14964..16076
                     /gene="msrB"
                     /locus_tag="VQ514_001070"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET2499836.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
BASE COUNT         4901 a         3052 c         3565 g         4614 t
ORIGIN      
        1 catatctacg gcaaggcgac actgacgaag tttgaattgg attttcgcag agtgtaaatt
       61 gttgaaacta ggttgtcata ccatgtatac cacttgggct ttggtagtag ctaactgcac
      121 taaatataat ataaggaggg ttttatgggg aaaggccatt ggaatcggaa aagagtttat
      181 agcattcgta agtttgctgt gggagcttgc tcagtaatga ttgggacttg tgcagttcta
      241 ttaggaggaa atatagctgg agaatctgta gtttatgcgg atgaaacact tattactcat
      301 actgttgaga aacctaaaga ggaaaaaatg atagtagaag aaaaggctga taaagctttg
      361 gaaactaaaa atgtagttga aaggacagaa caaagtgaac ctagttcaac tgaggctatt
      421 gcatctgaga agaaagaaga tgaagccgta actccaaaag aggaaaaagt gtctgctaaa
      481 ccggaagaaa aagctccaag gatagaatca caaacttcaa atcaagaaaa accgctcaag
      541 gaagatgcta aagctgtaac aaatgaagaa gtgaatcaaa tgattgaaga caggaaagtg
      601 gattttaatc aaaattggta ttttaaactc aatgcaaatt ctaaggaagc cattaaacct
      661 gatgtagacg tatctacgtg gaaaaaatta gatttaccgt atgactggag tatctttaac
      721 gatttcgatc atgaatctcc tgcacaaaat gaaggtggac agctcaacgg tggggaagct
      781 tggtatcgca agactttcaa actagatgaa aaagacctca agaaaaatgt tcgccttact
      841 tttgatggcg tctacatgga ttctcaagtt tatgtcaatg gtcagttagt ggggcattat
      901 ccaaatggtt ataaccagtt ctcatatgat atcaccaaat accttcacaa agatggtcgt
      961 gagaatgtga ttgctgtcca tgcagtcaac aaacagccaa gtagccgttg gtattcagga
     1021 agtggtatct atcgtgatgt gactttacaa gtgacagata aggtgcatgt tgagaaaaat
     1081 gggacaacta ttttaacacc aaaacttgaa gaacaacaac atggcaaggt tgaaactcat
     1141 gtgaccagca aaatcgtcaa tacggacgac aaagaccatg aacttgtagc cgaatatcaa
     1201 atcgttgaac gaggtggtca tgctgtaaca ggcttagttc gtacagcgag tcgtacctta
     1261 aaagcacatg aatcaacaag cctagatgcg attttagaag ttgaaagacc aaaactctgg
     1321 accgttttaa atgacaaacc tgccttgtac gaattgatta cgcgtgttta ccgtgacggt
     1381 caattggttg atgctaagaa ggatttgttt ggttaccgtt actatcactg gactccaaat
     1441 gaaggtttct ctttgaatgg tgaacgtatt aaattccatg gagtatcctt gcaccacgac
     1501 catggggcgc ttggagcaga agaaaactat aaagcagaat atcgccgtct caaacaaatg
     1561 aaggagatgg gagttaactc catccgtaca acccacaacc ctgctagtga gcaaaccttg
     1621 caaatcgcag cagaactagg tttactcgtt caggaagagg cctttgatac ttggtatggt
     1681 ggcaagaaac cttatgacta tggacgtttc tttgaaaaag atgccactca cccagaagct
     1741 cgaaaaggtg aaaaatggtc tgattttgac ctacgtacca tggtcgaaag aggcaaaaac
     1801 aaccctgcta tcttcatgtg gtcaattggt aatgaaatag gtgaagctaa tggtgatgcc
     1861 cactctttag caactgttaa acgtttggtc aaggttatca aggatgttga taagactcgc
     1921 tatgttacca tgggagcaga taaattccgt ttcggtaatg gtagcggagg gcatgagaaa
     1981 attgctgatg aactcgatgc tgttggattt aactattctg aagataatta caaagccctt
     2041 agagctaagc atccaaaatg gttgatttac ggttcagaaa catcttcagc tacccgtaca
     2101 cgtggaagtt actatcgccc tgaacgtgaa ttgaaacata gcaatggacc tgagcgtaat
     2161 tatgaacagt ctgactatgg aaatgatcgt gtgggttggg ggaaaacagc aaccgcttca
     2221 tggacttttg accgtgacaa cgctggctat gctggacagt ttatctggac aggtacggac
     2281 tatattggtg aacctacacc atggcacaac caaaatcaaa ctcctgttaa gagctcttac
     2341 tttggtatcg tagatacagc cggcattcca aaacatgact tctatctcta ccaaagccaa
     2401 tgggtttctg ttaagaagaa accgatggta caccttcttc ctcactggaa ctgggaaaac
     2461 aaagaattag catccaaagt agctgactca gaaggtaaga ttccagttcg tgcttattcg
     2521 aatgcttcta gtgtagaatt attcttgaat ggaaaatctc ttggttttaa gactttcaat
     2581 aaaaaacaaa ccagcgatgg gcggacttac caagaaggtg caaatgctaa tgaactttat
     2641 cttgaatgga aagttgccta tcaaccgggt accttggaag caattgctcg tgatgaatct
     2701 ggcaaggaaa ttgctcgaga taagattacg actgctggta agccagcggc agttcgtctt
     2761 attaaggaag accatgcgat tgcagcagat ggaaaagact tgacttacat ctactatgaa
     2821 attgttgaca gccaggggaa tgtggttcca actgctaata atctggttcg cttccaattg
     2881 catggccaag gtcaactggt cggtgtagat aacggagaac aagccagccg tgaacgctat
     2941 aaggcgcaag cagatggttc ttggattcgt aaagcattta atggtaaagg tgttgccatt
     3001 gtcaaatcaa ctgaacaagc agggaaattc accctgactg cccactctga tctcttgaaa
     3061 tcgaaccaag tcactgtctt tactggtaaa aaagaaggac aagagaagac tgttttgggg
     3121 acagaagtag caagagttcg tacattaatt ggtaaagaac caaaaatgcc gaaaacagta
     3181 ggttttgtat acagcgatgg tagtcgtgaa aaacttccag taacttggtc tcaagtcgat
     3241 gttagtcaag caggagttgt gactgttaaa ggtacagcaa atggacgtga ggtagaggct
     3301 catgtcgagg ttctagcaat tgcgaaagaa ttaccaacag ttaaacgtat tgctccaaat
     3361 actgacttga attctgtaga caaatctgtt tcttatgttt tgactgatgg aagtgtacaa
     3421 gagtacgaag tagatagctg ggagattacg gaagtagata aagccaaact ttcagtagct
     3481 ggatcacgta ttcaaatgac tggtcagtta gctggagaaa ctattcatgc aacccttgtg
     3541 gtagaagaag gaaatgctgc agcacctgta gtgccaactg ttactgttgg aggtgaggca
     3601 gtaacaggtc ttactagtcg acaaccaatg caatatcgta ctctatctta tggtgcccaa
     3661 ctgccagaag tcacagcaag tgctgaaaat gctgatgtga cagttcttca agcaagcgca
     3721 gcaaacggca tgcgtgcgag catctttatt cagcctaaag atggtggtcc tcttcaaacc
     3781 tatgcaattc aattcctaga agaagcacca aaaattgctc acttgagttt gcaagtggaa
     3841 aaagctgaca gtctcaaaga agaccaaact gtcaaattgt cggttcgagc tcactatcaa
     3901 gatggaacgc aagctgtatt accagctgac aaagtaacct tctctacaag tggtgaaggg
     3961 gaagtcgcaa ttcgtaaagg aatgcttgag ttgcataagc caggagcagt cactctcaaa
     4021 gcggaatatg agggagctaa aggccaagtt gaactcacta tccaagccaa tactgagaag
     4081 aagattgcgc aatctatccg tcctgtaaat gtagtcacag atttgcatca aaaacctact
     4141 cttccaacaa cagtaacggt tgagtatgac aaaggtttcc ctaaagctca caaagtcact
     4201 tggcaagcta ttccgaaaga aaaactagac tcctatcaaa catttgaagt actaggtaaa
     4261 gttgaaggaa ttgaccttga agcgcgtgca aaagtctctg tagaaggtat cgtttcagtt
     4321 gaagaagtca gtgtgacaac tccaatcgca gaagcaccac aattaccaga aagcgttcgg
     4381 acatatgatt caaatggtca cgtttcatca gctaaggttg catgggatgc gattcgtata
     4441 gagcaatacg ctaaggaagg tgtctttaca gttaatggtc gcttagaaga tactcaatta
     4501 acaactaaac ttcatgttcg cgtatctgct caaactgagc aaggtgcaaa catttctgac
     4561 caatggaccg gttcagaatt gccacttgcc tttgcttcag attcaaatcc aagcgaccca
     4621 gtttcaaatg ttaatgacaa gctcatttcc tacaataacc aaccagccaa tcgttggaca
     4681 aactggaatc gtactaatcc agaagcttca gtcggtgttc tgtttggaga ttcaggtatc
     4741 ttgagcaaac gctccgttga taatctaagt gtcggattcc acgaagacca tggagttggt
     4801 gcaccgaagt cttatgtgat tgagtattat gttggtaaga ctgtcccaac agctcctaaa
     4861 aaccctagtt ttgttggtaa tgaggaccat gtctttaatg attctgccaa ctggaaacca
     4921 gttactaatc taaaagcccc tgctcaactc aaggctggag aaatgaacca ctttagcttt
     4981 gataaagttg aaacctatgc tgttcgtatt cgcatggtta aagcagataa caagcgtgga
     5041 acgtctatca cagaggtaca aatctttgcg aaacaagttg cggcagccaa acaaggacaa
     5101 ataagaatcc aagttgacgg caaagactta gcaaacttca accctgattt gacagactac
     5161 taccttgagt ctgtagatgg aaaagttccg gcagtcacag caagtgttag caacaatggt
     5221 ctcgctaccg tcgttccaag cgttcgtgaa ggtgagccag ttcgtgtcat cgcgaaagct
     5281 gaaaatggcg acatcttagg agaataccgt ctgcacttca ctaaggataa gaacttactt
     5341 tctcataaac cagttgctgc ggttaaacaa gctcgcttgc tacaagtagg tcaagcactt
     5401 gaattgccga ctaaggttcc agtttacttc acaggtaaag acggctacga aacaaaagac
     5461 ctgacagttg aatgggaaga agttccagca gaaaatctga caaaagcagg tcaatttacc
     5521 gttcgaggcc atgtccttgg tagtgatctt gttgctgagg tcactgtacg agtgacagac
     5581 aaactaggcg aagctctttc agataaccct aactatgatg aaaacagtaa ccaggccttt
     5641 gcttcagcaa ccaatgatat tgacaaaaac tctcatgacc gcgttgacta tctcaatgac
     5701 ggagatcatt cagaaaatcg tcgttggaca aactggtctc caacaccatc ttctaatcca
     5761 gaagtatcag cgggtgtgat cttccgtgaa aatggtaaga ttgtagaacg gactgttgcc
     5821 caagccaaac ttcgtttctt tgcagatagt ggtacggatg caccaactaa actcgtttta
     5881 gaacgctatg tcggtccaga gtttgaagtg ccaacctact attcaaacta ccaagcctac
     5941 gacgcagacc atccattcaa caatccagaa aattgggaag ctgtgcctta tcgtgcggat
     6001 aaagacatcg cagctggtga tgaaatcaac gtaacattta aagctgtcaa agccaaagct
     6061 atgagatggc gtatggagcg taaagctgac aagagcggtg ttgcgatgat tgagatgacc
     6121 ttccttgcac cgagtgaatt gcctcaagaa agtacgcaat cgaagattct tgtagatgga
     6181 aaagaacttg ctgatttcgc tgaaaatcgt caagactatc aaattaccta taaaggtcaa
     6241 cggccaaaag tctcagttga agaaaacaat caagtagctt caactgtggt agatagtgga
     6301 gaagatagcc ttccagtact tgttcgcctc gtttcagaaa gtggaaaaca agtcaaggaa
     6361 taccgtatcc agttgactaa ggaaaaacca gtttctgctg tacaagaaga tcttccaaaa
     6421 ctcgaatttg ttgaaaaaga tttggcctac aagacagttg agaaaaaaga ttcaacactg
     6481 tatctaggtg aaactcgtgt agaacaagaa ggaaaaactg gtaaagaacg tatctttaca
     6541 gcgattaatc ctgatggaag taaggaagaa aaactccgtg aagtggtaga agctccgaca
     6601 gaccgcatag tcttggttgg aaccaaacca gtagctcaag aagctaaaaa accacaagtg
     6661 tcagaaaaag cagatacaaa accaattgat tcaagtgaag ctagtcaaac taataaagcc
     6721 cagttaccaa atacaggtag tgcggcaagc caagcagcag tagcagcagg tttagctctt
     6781 ctaggtttga gtgcaggatt agtagttact aaaggtaaaa aagaagacta gaaattttga
     6841 taccttcttt atcattaaag accaaagaaa aactcttgtc ctaaatagaa gataaagagt
     6901 ctggaacaaa atagttctca accacaaaaa agctagatat ttccaattgc ggaactctag
     6961 ctttttaatt ttgagtcgtt ctcttattgt atagtagatt gaaactagaa tagtacactt
     7021 ctacttctaa aacattgtta gaaatcaatt tgactgtcct tatcgatttg tcctgttctt
     7081 atttcatttc actatgtttt aggaggttat tggccataag taccaatccc atgtcaattc
     7141 tcacttgacg cttccctctc agattacatc tcttgtaacc caaacaagcc tttatctgcc
     7201 caaagacagg ttccacatca atcttgcgtt gagcgaaaat ctgtctacct tggggagata
     7261 aaagcgcctg acattcttta gctttcaagt tttgatagcg ttcgttcata tacagtccct
     7321 tttgagggac tgattcaggt tcatcagcgt agtaaacctt gatttcctgt tgaaagtctg
     7381 tctgtgtttt ctggtacttg atatggtgaa aacgatagca ccagccatca ggatgtgtgt
     7441 agctatcctc cttgtcatta tagtgccaat tcgctaagtt tttagctgac tgtttatacc
     7501 ctctcttttg ttccttatca aacatggcat atttaatcag aaggggagtg atttcttctt
     7561 gaaaatagac ctgtatctgt tcttgaagtt tggcggagaa tttctctgtc gttttcttcc
     7621 acacaaaact atacttgttg gcattggctt caatcttagt tccgtcaata aacaaacaat
     7681 ctaaggtcac taactcttcc atttttcaat ttttcgacca gagaaaatcc cttgtgaata
     7741 ggcaaataga agagtagata caagcatttt agggtgataa gacgggcgat caaaggcatg
     7801 atagaaggcg tggaagtgac aatcctccaa ggtattcacc actttttcaa tagtaaaaac
     7861 gagatgatct tgtggcaaga aggaactgat ttctagtggt aaagttgttt gatttgtgtt
     7921 atagtgaata tgcatgggat agcctttcta aatggtttat taagcaattc tattttacca
     7981 agactatcgc aaccatgcaa aaaagcaacg gcaaatccgt tgcctttttt ggagataaga
     8041 gactattgtc ccagactctt ttctttgtat aaaattgtat agtgttattg tctatactat
     8101 tttgtagaga aagcaaaaat ccctagaaaa tgcttaggga ttcgactatt tcattaagaa
     8161 caattcaaca atcatttcta tatacatgat aagggttaat aagaatcctg cacgccagaa
     8221 gaatttgata aatttaggat agtaaaagct gcgttttttc aaaaggaaga aaaagacgag
     8281 aataatggct aggatcgaga gggctagccc cagtctaggg aggaaattat gggtaaaggt
     8341 tttagctgta atcaagtaat actcaaatac caagactgga aaagccaaat ccgcaaagtt
     8401 tcgtcctagt ttttttaatc taaaaagttt ggttaggata atgcagacga ctaaggtcag
     8461 tatcaataat aaaatagatg ctaatttcat taaaatcata cccatattgt atcataaaaa
     8521 aaggctaatg gaaacgagaa acagaggaat ttataggaaa gtcaggcttt tgagattgtg
     8581 ggtgattttt gttataatga aagttataaa atcttataat accttgaatg attattatcg
     8641 aaaactcttt ggagaaaaga cttttaaagt ccctattgat gcgggatttg actgtcccaa
     8701 tcgtgatgga actgtggctc atggaggctg tactttttgt acggtttccg gttctggaga
     8761 tgccattgtg gcaccagatg cgcctatccg tgagcaattt tataaggaaa ttgactttat
     8821 tcatcgtaag tggccagatg ttcagaagta tctggtttat tttcaaaatt ttaccaacac
     8881 ccatgaaaag gtggaagtta ttcgagagcg ttatgaacag gctatcaatg agccaggtgt
     8941 ggtaggaatc aatattggaa cgcgcccaga ctgtttacca gacgaaacca tcgaatattt
     9001 ggctgagtta tcggagtgca tgcatgtgac ggttgaattg ggcttgcaaa ctacttatga
     9061 agcaacctct gacctgatta accgtgcgca ttcttatgaa ttgtaggtgg aaacggtcaa
     9121 gcgtttgaga aagtatccaa aaattgagat tgtttcccat ttgatcaatg gtttgcctgg
     9181 tgaaacccat gagatgatgg ttgaaaatgt ccgccgttgt gtcacggata acgatattca
     9241 agggattaaa ctgcatttgc tccatcttat gacaaatact cgtatgcaac gagattacca
     9301 tgaggggcgt ttacaattga tgagtcagga cgaatatgtc agggtcatct gtgaccaact
     9361 ggaaattatt cccaaacata tcgtcatcca tcgaattaca ggagatgcgc ctagggatat
     9421 gctgcttggg cctatgtgga gcctcaataa atgggaagct ctcaacagca ttgagatgga
     9481 gatgcgacgt cgtggaagtg ttcaaggatg caaggctgta aaacaggagt ttgaaaatga
     9541 aaagaccact tgagatggca catgattttt tggctgaggt cgtgacaaaa gaggatgtcg
     9601 tagtggatgc gactatggga aatggtcatg acacgctttt tttagccaag ctagccaagc
     9661 aagtctatgc ctttgatatt cagaagcaag ccttggaaaa gacccaagag cgtttgcatc
     9721 aggctgactt gacaaatgcc cagttaatct tgcaaggcca tgagacactg gaccagtttg
     9781 tgataaaagc taaggcaggg atttttaatc tgggctattt gccgtcagct gataagtctg
     9841 tcatcacccg accgcagaca acgattgagg cattagaaaa gctatgtggc ttacttgtca
     9901 aaggtggacg aattgctatt atgatttact atggtcatga aggaggcgat ctcgagagag
     9961 atgctgtctt ggattttgtg atccagttga accaacaaga gtacacagct gccatttacc
    10021 gaactttaaa ccaagtcaac aacccgccgt ttttagtgat gattgaaaaa ttagagagat
    10081 acagacatgg ataaacaata cctacacgaa aagctggatg ccatgcgcca gaattttgtt
    10141 gaatcaaccc accacgaacg atcggtgggc gtgctagacc aagcgcatat gagcaaaaaa
    10201 atgcttaaaa tcaagaaaaa attagttgct cttgaaatgg aacggtgcca gagaaaaatt
    10261 gagcacaaag actgttccaa gattgaccaa aaaatcaaag agcagaagga gatatttgaa
    10321 tcctgttgta aaaaagatta aggaggacgt gcgtggaatt actgatttac ctcatcctat
    10381 ttttactggt tttgattgtc tcaagtacaa ccaataagct cctgcccttt ttgcctctcc
    10441 ctttggtgca gattcttttg ggaattgtga ttggtctctt tttacccaat actgactttc
    10501 accttaatac ggagttgttt ttggcactgg ttatcggacc cttgcttttc cgagaggctg
    10561 aagaagcaga tgttacggct attttaaaac actggcgaat cattgtttat ctcatatttc
    10621 cagtgatttt tatctcgacc ttgagtttgg gtggcttggc ccatcttctt tggttcagcc
    10681 ttcccttggc agcttgcttg gctgttgggg cagcccttgg tcctacggac ttggtggcct
    10741 ttgcctctct ttcggagcgt tttagctttc ctaagcgcgt gtccaatatt cttaagggcg
    10801 aaggactctt gaatgatgct tctggtttgg tggcttttca ggtagctttg acagcttgga
    10861 caactggagc tttttctctg gggcaagcta gcagttcgct catcttttca atcctaggcg
    10921 gttttttaat tggattttta acggccatga ccaaccgctt cctccatacc ttcttgctaa
    10981 gtgtgcgcgc aacggatatt gccagtgaac ttttattaga attgagtttg cctctagtga
    11041 ccttctttct ggcagaagaa gttcatgttt caggtattat tgccgtcgta gttgctggaa
    11101 ttttaaaggc aagtcgcttc aagaaaatca cgctcctcga agcccaagtg gatacggtga
    11161 ccgagacggt ctggcataca gtgaccttta tgctcaacgg ttctgtcttt gtgattttag
    11221 ggatggagtt ggaaatgata gcagaaccta tcttgaccaa tccaatctat aatcctctac
    11281 ttttattgct atctctcatc gcccttacct ttgtcctctt tgtcattcgt tttattatga
    11341 tctatggcta ttatgcctat agaacccgac gcctcaagaa aaagctaaat aagtatatga
    11401 aggacatgtt tctcttgacc ttttcaggtg ttaagggaac ggtgtcgatt gctacgattc
    11461 tcttgatacc aagtaatcta gaacaggagt atcctctttt gcttttcctt gttgcaggtg
    11521 tgacgcttgt cagcttttta acaggtctct tggtcttgcc tcatctttct gatgaagagg
    11581 aagaaagcaa ggattatctc atgcatatcg ccattttgaa tgaagtaacg ctagagttgg
    11641 aaaaagagtt ggaagacacc agaaataaac ttcccctcta tgcggctatt gacaattatc
    11701 atggacgtat tgaaaatctc attttaagcc aagaaaacca ggatgatcaa gaagattggg
    11761 ctgctttgaa actcttgatt cttagtattg aaagtgatgg tttggaacag gcctatgaag
    11821 aggggaacat tagcaatcgt gcttaccgag tttaccaacg ttatctgaaa aatatagaac
    11881 aaggaatcaa tcgtaaactt gcctcaagac tgacctatta ttttcttgtt tccttgagga
    11941 ttttacgttt tctccttcat gaagttttta ctcttggaaa gaccttccgt agctggaagg
    12001 acaaggagca aagccgtctc cgtgctcttg attatgacca aattgcagag ctctatcttg
    12061 ccaatacaga gatgattatt gaaagtttgg aaaacctgaa gggagtctac agacgctctt
    12121 tgattagttt tatgcaggag tctcgtcttc gagaaacagc tattatcagc agtggtgcct
    12181 ttgtcgaacg ggttatcaat cgtgtcaaac ccaacaatat cgatgaaatg ctgagaggct
    12241 attatctgga gcgcaagttg attttcgaat acgaagaaaa acgattgatt acgactaagt
    12301 atgccaagaa attacgacaa aatgtaaata acttagagaa ctattccttg aaggaagctg
    12361 ccaataccct gccgtatgat atggtggaat tggtaagaag aaattagtta atactcttcg
    12421 aaaatctctt caaaccacgt cagcgtcgcc ttggattata tatgtgactg acttcgtcag
    12481 tttcatctac aacctcaaag cagggctttg agcaacctgc ggctagcttc ctagtttgct
    12541 ctttgatttt cattgagtat aagattataa atgaaggagt atgacatgaa aaagtggtgg
    12601 aaagagctga tagacaagcc tttattaaaa gcttttttgc attattatca agcatcagat
    12661 agtgagttga ccagtgtcgc agtagcctac tattggttga tttcgatttt ccccctgctt
    12721 ttggtggtgg tcaatatcct cccctatttt cagattcctg tgggagaatt tctgtgcttt
    12781 atgaaagacg ttctgccccc aagcctctat gaaggtgtgg aaaaaatagc cagagaagtt
    12841 ttgacgcaac cttcaacagg tttattgagt ttttctgttt tatcggcgtt atggagtttt
    12901 tcaaaatcta tgaatttctt gcaaaaagct tttaacaagg cttatggcgt cgaaaagagt
    12961 cgtggactta tttcccatca gatgctaagt ctcctagtca gttttggctt gcagctcctc
    13021 tttgcgtttg ccttgttttt gatcttattt ggtcagatga ttttggcttt acttgctcat
    13081 tattggacaa aagatggcat tatctatcag gctttacaag ggttggcagg tcctctgatt
    13141 tacgccctcc tctttgctat cttggtcatg ctttattatt ttcttcccaa tctatctaac
    13201 aggaaaatta gctataccct accaggcagt gcctttgttc tcttagtgat tttaggttta
    13261 ctaactctgt tctctagtta tctcaattac tatgtccatc atttggtgga tgtccgaatt
    13321 ttaggttccg ttcttcttgt tgttatgatg ttttggttta tcttgattgc taaaattgtt
    13381 atcctaggcg cggttatcaa tgccagtatg cagagtttga aagatccggt ctttaaaaaa
    13441 gactaactat ttatccatta caaaagcgca tagtatcaaa actttataga accttgatac
    13501 tatgcgcttt tgtgctttga cagttttttg tatagttttt gtccaagatt tctaaatcat
    13561 ctgattaaat tcctattttc ccctatcatt gtccctgttt tttcgaaaat ggaaggagta
    13621 taataaatct atcaataaaa tgataggagg aactttatgg gtcatatttt cttttttcta
    13681 agtgtctttt tggcagggat tctatccttc ttttctcctt gtatcttacc tttgttaccg
    13741 gtctatacag gagtgttact agatgataag gatggtgctc aggcttctag cggcaaattt
    13801 tcaatctcag ttactagttt attacgaact ctggccttta tagcaggaat ttcctttata
    13861 tttattttgt tgggctatgg agctggtttt ttaggcgatt tgctttatgc ttcttggttc
    13921 caatatctta ctggggcaat tattatcctt cttggtttgc accaaatgga gattctacac
    13981 tttaaggggc tttataagga aaagaggcta caactgcaag gacaggggca aaatggtaag
    14041 ggctatagtc aggcattttt attgggcttg acctttagtt ttgcttggac gccttgcgtg
    14101 gggccggttc tggggtctgt tttggccttg gcggcttcag gtggttcagg agcttggcag
    14161 ggagctggtc tcatgttggt gtatacgctg ggcttggcgc taccattctt gcttctagct
    14221 ctgacctcta gttatgtttt gaaacatttc cgaaaacttc atccctatct cggaatcctc
    14281 aaaaaagtgg gtggttttct cattattgtg atgggactct tggttctgtt tggaaatgct
    14341 tcaattttaa gtcaattatt tgaataaaat ggaaaggaat atcaatatga aaaaatggca
    14401 aacatgtgtt cttggagcag gttcgctcct ttgtttgacg gcttgttcag gcaagtccgt
    14461 gactagtgaa caccaaacga aagatgaaat gaagacggag cagacagcta gtaaaacaag
    14521 cgcagctaaa gggaaagagg tggctgattt tgaattgatg ggagtagatg gcaagaccta
    14581 ccgtttatct gattacaagg gcaagaaagt ctatctcaaa ttctgggctt cttggtgttc
    14641 catctgtctg gctagtcttc cagatacgga tgagattgct aaagaagctg gtgatgacta
    14701 tgtggtcttg acagtagtgt caccaggaca taagggagag caatctgaag cggactttaa
    14761 gaattggtat aagggattgg attataaaaa tctcccagtc ctagttgacc catcaggcaa
    14821 acttttggaa acttatggtg tccgttctta cccaacccaa gcctttatag acaaagaagg
    14881 caagctggtc aaaacacatc caggattcat ggaaaaagat gcaattttgc aaactttgaa
    14941 ggaattagcc taggaggtgt cttatgaatg ataagttaaa aatcttcttg ttgctaggag
    15001 tattttttct agccataacc ggtttctatg ttctattgat acgaaatgca gggcagacag
    15061 atgcctcgca aattgaaaag gcggcagtta gccaaggagg aaaagcagtg aaaaaaacag
    15121 aaattagtaa agacgcagac ttgcacgaaa tttatctagc tggaggttgt ttctggggag
    15181 tggaggaata tttctcacgc gttcccgggg tgacggatgc cgtttcaggc tatgcaaatg
    15241 gtagaggaga aacaaccaag tacgaattga ttaaccaaac aggtcatgca gaaaccgtcc
    15301 atgtcaccta tgatgccaag caaatttctc tcaaggaaat cctgcttcac tatttccgca
    15361 ttatcaatcc aaccagcaaa aataaacaag gaaatgatgt ggggacccag taccgtactg
    15421 gtgtttatta cacagatgac aaggatttgg aagtgattaa ccaagtcttt gatgaggtgg
    15481 ctaagaaata cgatcaacct ctagcagttg aaaaggaaaa cttgaagaat tttgtggtgg
    15541 ctgaggatta ccatcaagac tatctcaaga aaaatccaaa tggctactgc catatcaatg
    15601 ttaatcaggc ggcctatcct gtcattgatg ccagcaaata tccaaaacca agtgatgagg
    15661 aattgaaaaa gaccctgtca cctgaggagt atgcagttac ccaggaaaat caaacagaac
    15721 gagctttctc aaaccgttac tgggataaat ttgaatccgg tatctatgtg gatatagcaa
    15781 ctggggaacc tctcttttca tcaaaagaca aatttgagtc tggttgtggc tggcctagtt
    15841 ttacccaacc catcagtcca gatgttgtca cctacaagga agataagtcc tacaatatga
    15901 cgcgtatgga agtgcggagc cgagtaggag attctcacct tgggcatgtc tttacggatg
    15961 gtccacagga caagggcggc ttacgttact gtatcaatag cctctctatc cgctttattc
    16021 ccaaagacca aatggaagaa aaaggctacg cttatttact agattatgtt gattaatact
    16081 cttcgaaaat ctcttcaaac cacgtcagcg tcgccttacc gtatgtaggg tt
//
LOCUS       DAVBCW010000039        16132 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVBCW010000039 DAVBCW010000000
VERSION     DAVBCW010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16132
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.039"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(<1..733)
                     /locus_tag="VQ514_001071"
     CDS             complement(<1..733)
                     /locus_tag="VQ514_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET2499837.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLID"
     gene            complement(814..3327)
                     /gene="secA"
                     /locus_tag="VQ514_001072"
     CDS             complement(814..3327)
                     /gene="secA"
                     /locus_tag="VQ514_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HET2499838.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMATLTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYAQRYDVITADRDL
                     APEIQAMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            complement(3473..3760)
                     /locus_tag="VQ514_001073"
     CDS             complement(3473..3760)
                     /locus_tag="VQ514_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499839.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     MQKILLAFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            complement(3771..4421)
                     /locus_tag="VQ514_001074"
     CDS             complement(3771..4421)
                     /locus_tag="VQ514_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2499840.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKILAGYIKLDKGKVLQDGKVYGVKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQYVKDIQS"
     gene            complement(4425..5066)
                     /locus_tag="VQ514_001075"
     CDS             complement(4425..5066)
                     /locus_tag="VQ514_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499841.1"
                     /translation="MFKNFNNIWLKRKIVLLLRIVLMMILINYLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLVLLSLLVMLIVSRLEEKFKKG"
     gene            complement(5059..5850)
                     /locus_tag="VQ514_001076"
     CDS             complement(5059..5850)
                     /locus_tag="VQ514_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499842.1"
                     /translation="MKIPLLTLARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAIITYFFGTFSPLEWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYVVPYMVLKKYEDNV"
     gene            complement(6035..6370)
                     /locus_tag="VQ514_001077"
     CDS             complement(6035..6370)
                     /locus_tag="VQ514_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499843.1"
                     /translation="MEYLFKMIILLPCFYFFSWIEKANRDSKIFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLDYICFKKMLDLRRE
                     LGKSKGGRH"
     gene            complement(6510..6845)
                     /locus_tag="VQ514_001078"
     CDS             complement(6510..6845)
                     /locus_tag="VQ514_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499844.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(6936..8246)
                     /gene="der"
                     /locus_tag="VQ514_001079"
     CDS             complement(6936..8246)
                     /gene="der"
                     /locus_tag="VQ514_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HET2499845.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(8260..8973)
                     /locus_tag="VQ514_001080"
     CDS             complement(8260..8973)
                     /locus_tag="VQ514_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HET2499846.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            complement(8970..9866)
                     /gene="dnaI"
                     /locus_tag="VQ514_001081"
     CDS             complement(8970..9866)
                     /gene="dnaI"
                     /locus_tag="VQ514_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HET2499847.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTAWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(9867..11036)
                     /locus_tag="VQ514_001082"
     CDS             complement(9867..11036)
                     /locus_tag="VQ514_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HET2499848.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLEHPVYRRLLEKKIGDSAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFNPKEATIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEEAVLRIRER
                     GQKTKTQKQNQTAPAKTNIPKWSNPDYKNETSEETRLELERKKQELLARLEKGGD"
     gene            complement(11037..11510)
                     /gene="nrdR"
                     /locus_tag="VQ514_001083"
     CDS             complement(11037..11510)
                     /gene="nrdR"
                     /locus_tag="VQ514_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HET2499849.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            11650..12015
                     /locus_tag="VQ514_001084"
     CDS             11650..12015
                     /locus_tag="VQ514_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET2499850.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMIH
                     FGYEKEELPGVVSDYIKGV"
     gene            12020..12715
                     /locus_tag="VQ514_001085"
     CDS             12020..12715
                     /locus_tag="VQ514_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2499851.1"
                     /translation="MSLLVFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            12732..13499
                     /locus_tag="VQ514_001086"
     CDS             12732..13499
                     /locus_tag="VQ514_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499852.1"
                     /translation="MFWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAAYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVKELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(13852..15051)
                     /locus_tag="VQ514_001087"
     CDS             complement(13852..15051)
                     /locus_tag="VQ514_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2499853.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHLQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLEQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLDQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAGGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(15048..15941)
                     /locus_tag="VQ514_001088"
     CDS             complement(15048..15941)
                     /locus_tag="VQ514_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2499854.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
BASE COUNT         4858 a         3516 c         2915 g         4843 t
ORIGIN      
        1 tatcaattaa attgtcataa tagtagttgg gaatattttt tccatactca ttaagagcac
       61 cacgaagaat agcgtgtgaa agcgggttcc cagttgtttc tacttctttt cctaagaaaa
      121 ggaaactttg tttgttttga gcagcataaa tcccattaaa catgacattg agatttccag
      181 aggttggatt tttaaaacca gtagaaaatc ctgccccact tgccacaaag cggtgttgct
      241 ggtcttcaac tgaacgggca ccaactgcca tgtaagaaat caaatcatct acaagcggaa
      301 ggttttcagg ataaagcatt tcatcagcag ttgtcatccc tgtttctgtg atgacacgat
      361 agtgaagatg gcgaacggct ttgattccat tgataagact aggcgcttct gtcgcgttag
      421 gctggtgaat caagccctta tagccatctc cgttggtacg gggtttggca gtataaacac
      481 gcataaccat aaagatacga tctgccactt cttcttgtag gactgccaaa cgcttagcgt
      541 attcaaggac agcttcttcg ttgtcagatg agcatggccc gattaccaag agaattcgct
      601 gatcttctcc acgtataatg gcttctagct cttgatcgcg ctgtgatttc ctctccaaag
      661 cctgaccttc taattttgac aaggcacgaa cttcttctat atttatttta gagctttttg
      721 ctgtaaatac cataactcat ctccttataa agcaaacgga tacccagtaa aaatttactt
      781 ttcacaagat atccgcctct aaactatctc attttattgt cttttaccgt gacagttttt
      841 aaatttctta ccagaaccac atgggcaaag ttcattgcgt ccaatctggc tcaaatccaa
      901 atcttctggc atacttgctt ggtgagcagc gatattgcga gtcgctgttg tactgatatg
      961 acgttctgcc tgtggtcttt cttgttcatg aatttgtgct ttcatcatca agcgtgtcac
     1021 atcaaactca atcgaaccaa tcatatcatt aaacatacgg aaaccttctg cctgatactc
     1081 aacaacaggg ttgttctgag catagccacg aagtccaacc gcgttacgca attgatcaag
     1141 ggcatcgata tgatctgtcc acttgttatc caccactcgt agaatcaaaa ctttttggaa
     1201 ttctttaact gcttcttcat cgcgtagttt tgaaacctga ctatcgtaaa ccttcaaggc
     1261 acgttggaaa agctcttcct tgatggcctt atcagacaag cctgacaagt cttccatcgt
     1321 aatagaatct tcaggaagca agttgtactt agcaaagttc aaaattgcct ctagtttttc
     1381 atcttgtttg gcacgcgcat gaccatcaac gacacgttca atcgtgcgtt tgatcatagc
     1441 ctgaatttca ggtgccaagt cacgatctgc agtgatgaca tcgtaacgtt gagcatagat
     1501 aatctcacgt tgttcacgca tgacatcatc gtattgaagg acttgtttac gggtatcgta
     1561 gttatttcct tcgacacgtt tctgagctgc ttcaacctga cgcgtcaaca tgcgagactc
     1621 aatcgcctct tcagacatgt tcaaacgttc aaagattccc ttcaagcgtt cagaaccaaa
     1681 acgtttcatc aaatcatctt caagagatag gtagaattgt gactcacctg gatctccttg
     1741 acgacctgaa cgtccacgaa gctggttatc gatacgacga ctttcatgac gttctgtacc
     1801 aataacacaa agtcctccaa gttcacgaac accttcacca agcttgatgt cggtaccacg
     1861 acccgccatg ttggttgcga tggtaacggc accacgttga ccagcattca tgatgatttg
     1921 ggcttctcta tagtggtttt tggcattcaa gacttcgtga ggaacaccag ctgcaaccaa
     1981 tttcttagaa atgtagtcac tagtttcaac cgctactgta ccaaccaaga caggttgacc
     2041 cttttggtaa cgagccttaa cgtcttcgac aaccgcttta aacttagatt cgatacttgc
     2101 ataaagaagg tctgagtggt caatacgttg aacaggacgg tttgttggga ttggaataac
     2161 acgaatgttg tagatttcac ggaattcttc ttcctcagtc ttacctgtac ccgtcatacc
     2221 agacaatttc ttgtacatac ggaaaaggtt ttggtacgtg attgaggcag atgtcttggt
     2281 ttcatcctgg attggcacac cttctttggc ttcaatagct tggtgcaatc catcagaata
     2341 acgacgacct tccatggtac gacctgtaaa ttggtcgaca atcaagattt cttgctcttc
     2401 gctcaccaca tagtcaatat cgagaagcat gatgtagttg gcacgaaggg cgttatcgat
     2461 aaagtgagtc aaagccacgt tttcgatgtc atagaggttt tcaagtttga agtagctttc
     2521 agccctgtca atccctgaat cagacaaacc aatagtctta gactgcacat cgatgatgta
     2581 gtcatctttg ttcaaagatt ttacatagtg gtctgccatg tgatacaact gactggtttc
     2641 aaccgcattg gcacctgata caatcaaagg tgtacgagcc tcgtcaatca agatagagtc
     2701 aacctcatcg accaaggcat agttaagcgg acgttgtacc atgttttcgg cgcgaacgac
     2761 catgttgtca cgaaggtagt caaatccgat ttctgagtta gttgagtaag taatatcaca
     2821 ctcataggct tcttttttct ccattggaga tttggtagcc aagttaatcc ctactgacaa
     2881 accaagccaa gagtacaatt cacccatctc agtcgcgtca cgttctgaca ggtattcatt
     2941 aaccgtaact acgtgaaccc ctttacctga aagggcattg aggtataccg gcatggtcgc
     3001 agtcaaggtt ttcccttccc ctgtacgcat ctctggcacg tcaccatggt gaagaacaat
     3061 cccccccatg acctgaacct tataagggaa gagacctagg acacgtttgg caccttcacg
     3121 gacaaccgca aatgcttcgt aaagcaatga atccagtgat tctccatttt gataacgttc
     3181 cttaaattca actgtttttg cttttagttg gtcgtcagtc aaagtagcca tttggtcttc
     3241 gtatttgaaa accttgtcag ccatcttttc cagacgacgg atttctcctt tatcattttc
     3301 gataattgtt tttaaaatat tagccatgtt tttccttact ttaaattccg aatattttag
     3361 aatgttcctt taattttagc acaattactg attattttca aggaagaatc cccattttga
     3421 aaaggaaaaa gaggttagtt ttcaagctaa cctctccgac ttttatgact gtttaattgc
     3481 caaaaatcgg caaaatggca aatgtgataa ataagttaat ccaaagagaa agacagcaaa
     3541 tcagtccaaa aacaaagaga ctgactttct tggacagcaa ggccatgaga atggacagaa
     3601 ttccaaaagc tagcaagatt ttctgcatga tgaagatgtc aatccttacc cccagaatcg
     3661 ctctatccca agggataaag aagaaagaca gccagatcag cagaactaaa ccaatataaa
     3721 ccttggaata gcgtgtaata aatgatttta gatgtgccat aagccatctc ctaggattgt
     3781 atatccttta catattgtat atgtccattt tcgacaacta atacatctgt acaaaggtct
     3841 gaaatatctt ccgatatgtg cgacgtcagg ataaccagac cattttcttt tttcaggtaa
     3901 gataaaatga cctgtttggt taacctcaca ctcttctcat ccaaagcatt cataggttca
     3961 tcgagaaaga gtatagaagg agaggaaata aaggcttgaa tcaaggccat tttttgcttt
     4021 gttcccaagg ataaatgacg gtattcaacg tcttcaaatt cctgtaaatc atagtattga
     4081 atccaatagg caattctttt ttccgtaact ttagatgaaa aataccttaa cagttccaaa
     4141 ttttctctca gggataaatg agataaaaac tcgacttttt caattaaaat tcctgcatcc
     4201 tgaatataat gattttttac cccgtaaact ttaccatctt gaagaacttt tcctttgtca
     4261 agcttaatat aaccagcaag tatctttaaa agaaccgtct tgccagatcc attatcacct
     4321 ttaagtccat aaatttttcc tgattgaaac accaaattca actgatttaa aataggtctt
     4381 ttcccataaa ttttctgtcc atttataatt tcaagtctca tcgtttatcc tttcttaaat
     4441 ttctcttcca agcgactaac aatcaacata actaataagc ttaagagtac taaatacact
     4501 gttacaatat gatccatcat ccattcttgt cccacaaatg ccaaaaaata gagaaaaaaa
     4561 gaaaaaccag cgcccgcact aatcagtgct atcagataag acagtagtaa gtacaacatc
     4621 attaactgag caagtaaaaa gaaaagtgcg actgttttga aatgaattcc tagcggaagt
     4681 aaaccaagaa accagacaaa caaatcttga gtaaaaaatt tagaaatgac aaccaatcgt
     4741 ttccttgtat aatcgaaaaa ggagcttgat tgatagcgaa tcaagtgatg gtaatggtct
     4801 gccaaatcac tttcaagtaa aatgacagag agccatccta ccatgaaaag cgaaaggttt
     4861 aacaagagtt tggggatttc taaattcgct tcagaatagt cattatagga aaaaattgaa
     4921 atcaattctc tcttgaaaaa gataacagca tcctgctttt gaaccgctgt tgacaataga
     4981 tagtttatca aaatcatcat cagaactata cgaagtagta aaacgatctt tctctttagc
     5041 caaatattgt taaaattctt aaacattatc ttcatatttt ttaagtacca tataagggac
     5101 aacataaagg atataaggag taaagagttt catcaaactt acatccccat agctagcttg
     5161 catcaaactc gtcataggaa ccatatagta aggcaaggca ctatagagca gcatagaacc
     5221 aagccaaaga aacatcaaat aggtgattgc cgaacgagtc acattcccca cataatcaac
     5281 tatttgtaaa aaatagattg cattgatgaa aataccgatt agtaggacac aagtaaagaa
     5341 caaatagctt tttatctctc catctaggag tctttgtaaa ccactcccat cagaaaatag
     5401 agaattccat tcaagaggag aaaaagtccc aaagaaatag gttataattg caattatcag
     5461 cacagtcact aaatatatca aacaagggat acttgcaact tgcaaagtca actttctttt
     5521 taacccttga tagctcactt ctcttccaat actcaatcgt aaaactttat ttttcagctc
     5581 aatatattga aaacctagca aaacaatgat gattggaata ataaatgatt gataaaaacc
     5641 tagattgaac tggagaatac gaaaatctaa tgctgatttt aataagtcat ttttaattgc
     5701 ttgtccatcg aatttagcta gatcgggcgc atgaatatca aaaaagaaat aagtcatcaa
     5761 aacatcacga taaaccaaag caagaaaaag caaagtaagc aagacataaa caaatttatg
     5821 ccttgccaaa gttaagagag gaatcttcat aagccacctc ctacaaatag aaagtaatat
     5881 aatactcaat gaaaatcaaa gagcaaacta ggaaactagc cgcaagttgc tcaaagcact
     5941 gctttgaggt tgtagataag actgacgaag tcagttacat atataatcca aggcgacgtt
     6001 gacgtggttt gaagagattt tcgaagagta taaatcaatg ccttccgcct ttagacttcc
     6061 ctagttcccg tctcaagtca agcatttttt tgaaacagat atagtctaac cagtcactag
     6121 caaaagcgat aagtagtaat aatagccaaa taccccataa cttgatatct gtcaaatttc
     6181 tcaagacgat attgaaaaac agaactgaaa aaactgtcca agcaaggcta aaaagagcat
     6241 agagggtgat gtaaatccag taaaaatagt aaaaaattgg gaaaatctta ctatctctgt
     6301 ttgccttttc aatccagctg aaaaagtaaa aacagggtaa taagattatc attttaaaca
     6361 agtattccat cacaaacctc ctcttttaaa ttgttttctt tagtttcgtt ctattaaatg
     6421 ttattaccaa tataaacctt ggaatagcgt gtaataaatg attttagagg taccataagc
     6481 cacctcctac aaatagaaac cgatataaat caatgccttc cacccttaga cttccctagt
     6541 tcctgtctca agcgaaacat ttctttgaaa caggaataag ttaaccaatt cataccaata
     6601 gctagcagaa taaaaagaaa ccaaatgccc cataacttga tatctgtcac atttctcaag
     6661 acggtattga aaaacagaac tgaaacaact gtccaagcaa ggctaaaaag agaatagaag
     6721 gggatgtaaa accagtaaaa ataataaaaa attggaaaaa acttactatt tctgttggcc
     6781 ttctcaatcc agttatcaaa ataaaagtac ggtgctaaaa gtaagaattt aaacaaatgt
     6841 tccatcaccg acatcaccct ccttcttttg atagcgtttt ctattatttt attatatcaa
     6901 aaaaatccgg aactgtcatt ccagattcta cttttttatt tgcgttttct tgcgatgaga
     6961 tgaatcggtg ttccctcaaa aacaaaggcc ttgcggattt gattttccaa gaaacgcagg
     7021 taagaaaagt gcatgagttc ttcttcattg acaaagatga caaaggttgg tggtttggtt
     7081 gccacttggg tcgcatagaa aatcttgaga cgttttcctt tgtctgtcgg tgttgggttg
     7141 atggcaatgg catccatgat gacatcgttc aagacagctg atggaatacg tgtattttga
     7201 ctttcgctga tttgcttaat catctcagga agtttgtgga gacgttgctt ggttaaagct
     7261 gatacaaaga taatcggtgc gtaaggcagg tattggaact gctcacggat atcttcttcc
     7321 cagtttttca tagtgtggtt atctttttca agcgtatccc acttgttgac cacgataatc
     7381 atccctttac cagcttcatg ggcaaatcct gcgatacgct tgtcgtactc acgaatgcct
     7441 tcttccgcat tgatgaccat caagaccaca tctgaacggt caatagcacg catggcacgc
     7501 ataacagagt atttctcagt attttcataa accttaccag acttacgcat accagccgta
     7561 tcaatcatgg taaactcttg accatctgta tctgtaaagt gggtatcaat ggcatcacga
     7621 gttgttccag caacaggact agcaataaca cggtcttctc ccaagatagc attgatcaag
     7681 cttgattttc caacgttagg acgaccaatc aagctaaact taatgacatc tggattttct
     7741 tcctcatatt catttggaag attttctacg atcgcatcta gcacatcccc tgtaccgatt
     7801 ccatggacag atgagatagg caatggttca cccaaaccga gagcatagaa atcatagata
     7861 tcatttctca tctcagggtt gtccaccttg ttgactgcga ggataactgg tttgtgggtc
     7921 ttataaagct tacgggctac gtattcgtct gcatcggtaa ttccttcctt accggacacg
     7981 acaaagacga taacatctgc ttcttccatg gcaatttctg cctggtgctt gatttgttcc
     8041 atgaaaggag catcgacatc atcaattcct cctgtatcaa tcatgctaaa agaacgattg
     8101 agccactcac ccgttgcata aatacggtca cgtgtcactc cttcgacatc ttctacaatg
     8161 gagattcgct caccagcgat ccgattaaat agggttgatt tcccaacatt gggacgtcct
     8221 acaatggcaa tagttggtag ggccataatt tctcactttc tacaataatt tcttctgttc
     8281 aagatttttt ctagttgagc ttggttcagc ttgaccaaac tgttctgcta ggcgctgact
     8341 ccagcttgtg gtcgcacgcg ccccagcata gtcagcctga acacggtcat aagcttggat
     8401 tgcctcagtt gactgttctt ggtattcttc ctcaaagaca acattctcta gtggcagtct
     8461 cggtttcata tcatgatgtt gatttggcac acccagtgcc atcccaaaga cagaataggt
     8521 gtagtcaggt aggttaaaga gctctgccac ttcttcagac ttgtatcgaa ccaaaccgat
     8581 aatcacacca ccatagccca agctttcagc tgccaacaag gcgttttgtc cagcaagagc
     8641 tgcatcgacc gaactaatca agagaccttc cacaccttgg ggttggaagg tgtcggtatg
     8701 aagtcgggct cccttttctg ctcggttcaa atctccgaca aagagaagga aaacagcaga
     8761 ctggcgaatg gcttcttgag gtaccaattc atacaaggca tctttcttct cttgacttcg
     8821 taccacaatc acagagtagg attggaaatt cttccaagat gatgccatct gggctgctgt
     8881 caaaatctca tttaagtcta cttggggaat ttcttgctct ttaaacctgc gcactgaagt
     8941 atgagccttc atcaatttaa tggtttctgt catcgacggt ttactccttc taaacgagtc
     9001 tcctcagcca aataacggat gcgttccatg acccgtctgg cttcccaggt ttcgtcattt
     9061 ccatgtttca ctttcgcaaa atgcttctcc aaatcttcaa agttgaagtt ggatgtgaaa
     9121 aaggtcggta aattttcctg catccgatat tggagaatga cctgcaggat ttcgtcacgc
     9181 acccaagcgg ttgattgctc ggcgccaata tcatctaaaa tcaggacctc agacagctta
     9241 atctcatcca ccaaggtctt aacattgcca tcactgatag catttttgac atcaatgaca
     9301 aagctaggat agtggaggag agttgatgaa acaccacgtt tttctgataa atcatgagct
     9361 aaggccgcca ccatgaaact tttacccaca ccaaagtctc catataagta aagacctttt
     9421 cgaatagctg gatattgctc cacaaaggct aatagttttt caaaaactgg caagcgtccc
     9481 aaatcatcca aatcaacttg agccaaacta gcttttttga gactggctgg tagattgatt
     9541 agcttgagac gattcttaat agccgcttct ttttcagccg cgattagctc aggagtttct
     9601 tcatatgaaa catctgcata gccatgattc ttaaccaaaa tcggcttgta gcctttggca
     9661 atataatccg tatccccacg gagaaacttg tcacgctcgg tgatgtactg attaaacttg
     9721 gagatactgc gatttaattc ctctggagtc aaggattctt gctggataaa agccgcaaca
     9781 tcagggtcct tcatgatttt ctggaccaaa tcttgataat aaaaacggct gggttgacgt
     9841 ttgagtacgt ctccgacact ttccatctaa tctcctcctt tttctaatcg agctaatagt
     9901 tcttgtttct tacgttctag ttccagacga gtttcctcgc tggtttcatt cttataatct
     9961 gggttactcc atttaggaat attggttttt gctggggcag tctgattctg tttttgtgtt
    10021 tttgttttct gccctcgctc acgaattcgt aaaacggcct cttctgctga atgtatcttt
    10081 tgataggcat agtcattagc caccttcatg gcatatttct cattgatgtt tgccgaatcc
    10141 accttattaa aggtcaacaa gagaatgata ttgatgactt cgtccagcaa gcccaagcca
    10201 gccatctgtt gcaaaagttc tctttctgtt tgggtaatgg tccccttgcg tgtttgcttg
    10261 atttctgcta agaactgcag ggcagtctta cttttagctt ccttgataat ggttgcttcc
    10321 ttaggattaa agtcagagga aactggtttt tgagcaattt tttcacgcat gcgtttggtt
    10381 gaaataacct gagaaacagc tgttgacttg gccaattgat aggtttcaaa ccaagtccat
    10441 ttcttctcct cggcaatagc aaagaggttt aagacatcgg actgctcatc cgcaaaacga
    10501 agtccatctc gagccatcag ctggcgaaaa tgttccaagt caaaatcatt ggccactttc
    10561 ttcttgagac caaggtcttc ttgactgcct agttctgcca attctggaaa gacttgattg
    10621 agtgagacag gtatttcttc accatcagca ctttcaactt tcaaatcctc cacagcagag
    10681 tcgccaatct ttttctctaa gagtctgcga taaacaggat gctccaagaa gtcttgacta
    10741 gatagaggag catggagggc tagctgataa acatccccct tttgatagag ggtcaagaga
    10801 ttaaaagcag ataagatttt caatgatttt atcagtctat ccatcccaaa gttgagatga
    10861 ttgaggatac ttgaaaaaag atattccttt ctaccattat cccaaaaact gattgtataa
    10921 agataaaggc tcagtgcctc ctgaccgata atcgggaggt agcactgtac cagagatgag
    10981 gtatcttgcg acacccgatt attctttaga taagaaaaac ggtcaattgg cttcatttat
    11041 ctttcctttt tctttttaga ggactgggtg atttgttgga gcaagctctc taactcactg
    11101 acatccttaa aactacgata gacactagca aaacgtacat aggtaatctc gtccaattca
    11161 gccaactcct ccatgacgag tgaaccaatg tcctcacttt gaatttcatt ttcatttcga
    11221 ccacggagtt tctgttcgat acgattgact accatgttga tttcatcact tgacacagga
    11281 cgtttctggg ctgagcggat aatcccatta aagattttat ctctggagaa ttgttcccgt
    11341 gtaccatctt ttttaacaac cactaaggtt ctttcttcta ctcgttcgta ggttgtaaaa
    11401 cggtgttggc attcgtcgca ctcacgtctt ctacgaatgg tgttcccttc ttctgcttgg
    11461 cgactatcga taacacttga cttggtagcc ccacattttg gacaacgcat cctttccctc
    11521 cttatcgttt tcttttcatt ataccatttt ttaagcgatt cccaaaacaa ttcttctttt
    11581 tgcttgacaa gttttttgtt ttgttgtatt atttagttaa gacaaaagga taagagaaag
    11641 gagactaaga tgtcctggac atttgacaac aaaaaaccca tctatttaca gattatggag
    11701 aaaatcaagc ttcagattgt ttcccataca ctggaaccca atcaacaact tccaaccgtg
    11761 agggagctag ctagcgaggc tggtgtcaat cccaatacca tccaaagagc cttatcagac
    11821 cttgaacgag aaggatttgt ctacagcaag cgaacaactg gacgatttgt gactaaggat
    11881 aaggagctaa tcgctcagtc acgcaaacaa ttatcagaag aagaattgga acacttcgtt
    11941 tcctccatga tccattttgg ctatgaaaaa gaagaactac caggcgtagt cagtgattat
    12001 attaaaggag tttaagccta tgtcattact agtatttgaa aatgtatcca aatcatatgg
    12061 agcaacacca gcccttgaaa atgtttctct tgacattcca gctggaaaaa ttgtcggcct
    12121 tcttgggcca aacggctcag gaaaaacaac cctgattaaa ctaattaatg gcctcttaca
    12181 accagatcaa ggacgtgtcc tcatcaacga catggaccca agcccagcaa ccaaggccgt
    12241 tgtagcttat ttgcctgata cgacctatct caatgagcaa atgaaggtca aagaagccct
    12301 aacctacttc aagaccttct ataaagattt caatcttgaa cgcgctcatc atctacttgc
    12361 agacctgggc attgatgaaa atagtcgtct caagaaacta tcaaaaggaa acaaagaaaa
    12421 ggttcaactg attttggtta tgagccgtga tgctcgtctc tatgttttgg atgaacccat
    12481 tggtggggtg gatccagcag cccgtgctta tatcctcaat accattatca acaactactc
    12541 accaacttct accgttttga tttctaccca cttgatttct gatatcgagc caatcttgga
    12601 tgaaattgtc ttcctaaaag acggaaaagt cgtccgtcaa ggaaatgtag atgatattcg
    12661 ctacgagtca ggtgaatcca ttgaccaact cttccgtcag gaatttaagg cctaagcaaa
    12721 ggagattatt tatgttttgg aatttagttc actacgaatt taaaaatgtt aacaagtggt
    12781 atttagccct ctacgcagcc gtgctagtcc tttctgccct catcggaata cagacacaag
    12841 gctttaaaaa tctaccttac caagaaagtc aggctactat gctacttttt ctagctacag
    12901 tctttggtgg cttgatgctt acacttgcga tttcaaccat tttcttgatt attaaacgct
    12961 tcaaaggtag tgtctacgac cgacaaggct atctgacttt gaccttgcca gtttctgaac
    13021 accatatcat cacagccaaa ctaatcggtg cctttatctg gtcattgatt agcaccgctg
    13081 tattggctct aagtgctgtt attattctgg ctttaacagc tccagaatgg attcctcttt
    13141 cttatgtgat tacatttgta gaaacacatc tccctcagat ctttcttaca ggtatatcct
    13201 tcctactaaa taccatttca ggaatcctct gcatctacct ggctatttcc attggacagc
    13261 ttttcaatga ataccgtaca gcactcgctg ttgcagccta cattggtatc caaatcgtca
    13321 ttggatttat tgaacttttc ttcaatctta gttctaattt ctatgtcaat tcactggtag
    13381 gactcaatga ccatttctat atgggagcag gtatagccat tgttaaagaa ctcatattca
    13441 tagctatctt ttatctcgga acctactaca tcttgagaaa taaggttaat ttgctttaaa
    13501 taatttttac ctagatatgt aacatactca tagaacaaaa gagaccaggc aaaaagtctt
    13561 taaaattaga aaacgcatag tatcaagtgt taaaaatcct tgatattatg cgttttttgt
    13621 gagctgactt atttcctttc actatatcgc aaaatgaaat aagaacggaa cgatgggatt
    13681 ttggaattca aatcaattta taagaatgtt ttagaagtaa tattatccta ttccagattc
    13741 agttcactat acaattgagt tttcaagcaa cctgtttaca taatgtgtac atcattaggt
    13801 tcgtgattcc acccttttca cctttaaaaa cctcgctttc gcaaggctct tctatttata
    13861 agataaggca cgtttaaagg ttttccaaat ccctaaatca tccgtttgaa gaacgagact
    13921 agcatacatg cgtccgataa atcctgttgc taccaccgca aaaatcactg taatagcaag
    13981 tgaaatccat gcttctgctc cccccgcata gccattaatc gttcgaaacg gcataaagaa
    14041 ggtcgaaata aagggaatat aagaaccaat cttcaagagg agattgtcac cagctgcacc
    14101 tagagctgtc actccaaaaa aaccacccat aatcaaaatc atcaaaggcg acaaggcttt
    14161 ccctgagtcc tcaggacgag aaaccataga tcctaggaat gctgccaaga ctacgtacat
    14221 gaaaagactg atcaaaataa agagcaaggt attcagtgag atagcatctc ccaagtgatc
    14281 caaaatacca gactgatcca agaatggcaa atctttaaag agcaaaacgg cagccagacc
    14341 acctacaaca tagatcccaa tatgcgttaa aatcactaga aacagagcca tcatccgcgc
    14401 atagaaatag tgacttgccc ttatgctaga aaaaacgact tccataattt tggtgccttt
    14461 ttcactggca acttcctgag ctgttacacc cgcataggta atcagaatca tataaagaaa
    14521 gaatcctaag gcacctgctg caattgtttg aataaacttt ttattttcct tggcttcatc
    14581 aatcttttct gtgaattgaa ttgtctgctc taagcgtttt tcctgctctt gagacaagga
    14641 agcagttgaa cgattaagct gattttgcag ttcattgagt gtacctgtaa cctcaaattt
    14701 aattccattt tcaagcgatg tttcgccatg ataaactgcc tttagaacac tatcttcttg
    14761 atcaatggtc aaataacctt ttaatttttc ttctttaatt gcttctttgg cacttgcttc
    14821 gtctttatag tcgaagttaa caccatttac attcttcagt ccttctgcta cagatggcac
    14881 tgttgtcact actgccactt tattattttt agccatagaa gaaccttgga gatgcccaat
    14941 tcctacagag attcctaaaa agaggaacgg cgaaatcacc ataaagaaga aactccatga
    15001 ctcgacatgt cgaagatagg tttccttgat tacaacccac atatttctca tacttccact
    15061 cctgattcta gtttaaagat ttcatcgata gttggcgctt gttggtcaaa tgttgcgata
    15121 tattgacctt gagtcaagat tgggaagagt tcccttccag cgctctcatc ctccaaaatc
    15181 aatttccaac tgccttgttt ggtcaagctc acctgtttga catgaggaag attttccaat
    15241 tcttccttgc ttcgttcact tgaaacaaag agacgcgttt tcccgtattg attgcggaca
    15301 tcctgaactg gtccgtgcaa gaccacacgg ccatctcgga tcatcagaat atcgtcacaa
    15361 agttcctcaa cattggtcat gacatggtca gaaaagataa tggttgctcc gcgctctttt
    15421 tcctgaaaaa tgacttgttt gagcaattct gtattaactg ggtccaatcc actaaaaggc
    15481 tcatccaaga taatcaggtc tggttcatga atcagagtaa taatgagctg aatcttctgc
    15541 tgatttcctt ttgacagact cttgatttta tctgtcagct ttcctttcac ttccaacctc
    15601 ttcatccatt gagggagttt ttctttgact tctttggcat ccatgccttt tagagtcgcc
    15661 aagtagcgaa cttgttcaag aactgtcaat ttaggcatga gactgcgttc ttcaggcaga
    15721 taaccaatcc gagcataggt ctcctgacga atatcctgac catccagacc gatttctccc
    15781 tgatattcta ggaatttcaa aatactatgg aaaatcgttg tttttccagc accatttttt
    15841 ccgactagtc ccaaaatacg acctggtcgc gcttgaaagt caataccaaa caaaacttgc
    15901 ttggagccaa aacttttctc tagacttctt acttctagca tctttcacct ccgaaatttc
    15961 ttgcactcat tatactcctt tttgatagcc tttacaatgt ttttgtccat ttttagaaga
    16021 ctattgctgt gtaaaatatg gcctggagca cttttatact caatgaaaag caaagagcaa
    16081 actaggaagc tagccgtagg ctgctcaaag tacagctttg aggttgcaga ta
//
LOCUS       DAVBCW010000040        15996 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVBCW010000040 DAVBCW010000000
VERSION     DAVBCW010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15996
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.040"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(115..1356)
                     /locus_tag="VQ514_001089"
     CDS             complement(115..1356)
                     /locus_tag="VQ514_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HET2499855.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITAYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFIDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            1459..2568
                     /locus_tag="VQ514_001090"
     CDS             1459..2568
                     /locus_tag="VQ514_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET2499856.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
     gene            3102..3449
                     /gene="ssrA"
                     /locus_tag="VQ514_001091"
     tmRNA           3102..3449
                     /gene="ssrA"
                     /locus_tag="VQ514_001091"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(3632..3719)
                     /locus_tag="VQ514_001092"
     tRNA            complement(3632..3719)
                     /locus_tag="VQ514_001092"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3683..3685),aa:Ser,seq:gga)
     gene            3881..4621
                     /locus_tag="VQ514_001093"
     CDS             3881..4621
                     /locus_tag="VQ514_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET2499857.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNITVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            4618..5529
                     /gene="pfkB"
                     /locus_tag="VQ514_001094"
     CDS             4618..5529
                     /gene="pfkB"
                     /locus_tag="VQ514_001094"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HET2499858.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVESVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDVVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            5526..7478
                     /locus_tag="VQ514_001095"
     CDS             5526..7478
                     /locus_tag="VQ514_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2499859.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTDHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGALYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            7642..9867
                     /locus_tag="VQ514_001096"
     CDS             7642..9867
                     /locus_tag="VQ514_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HET2499860.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEIAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(9912..10340)
                     /locus_tag="VQ514_001097"
     CDS             complement(9912..10340)
                     /locus_tag="VQ514_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499861.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTVATIASILGLITASITIMN
                     ALDNNKNIKKEPTTSQTTTIVKEIQKESPKENTSPTKETNTSQEKTQQEETPKSSVKE
                     EKKEDQKTATQDSSTPASSKPATENEKQSNAPTSENKSNQ"
     gene            10478..11620
                     /locus_tag="VQ514_001098"
     CDS             10478..11620
                     /locus_tag="VQ514_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET2499862.1"
                     /translation="MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWLIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKSQGFEVDFAPVDKKGLVDVEALAGLIRHDTILVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     NYPDIFVFSDEENFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            11629..12843
                     /gene="thiI"
                     /locus_tag="VQ514_001099"
     CDS             11629..12843
                     /gene="thiI"
                     /locus_tag="VQ514_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HET2499863.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYTQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSSVQEIMRDIYKE
                     GMTFKISSKRSDHNFELDSRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAY
                     LSYETIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            12978..13802
                     /locus_tag="VQ514_001100"
     CDS             12978..13802
                     /locus_tag="VQ514_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET2499864.1"
                     /translation="MNQSYFYLKMKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPV
                     VYFHDGQNVFNSKESFIGHSWKIIPAIKRNPDISRMIVVAIDNDGMGRMNEYAAWKFQ
                     ESPIPGQQFGGKGVEYAEFVMEVVKPFIDETYRTKADCQHTAMIGSSLGGNITQFIGL
                     EYQDQIGCLGVFSSANWLHQEAFNRYFECQKLSPDQRIFIYVGTEEADDTDKTLMDGN
                     IKQAYIDSSLCYYHDLIAGGVHLDNLVLKVQSGAIHSEIPWSENLPDCLRFFAEKW"
     gene            13824..14563
                     /locus_tag="VQ514_001101"
                     /pseudo
     CDS             13824..14563
                     /locus_tag="VQ514_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            14576..15742
                     /locus_tag="VQ514_001102"
     CDS             14576..15742
                     /locus_tag="VQ514_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET2499865.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIGRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMEQMIADFGQRQE"
BASE COUNT         4648 a         2998 c         3512 g         4838 t
ORIGIN      
        1 acctgcggct agcttcctag tttattcttt gattttgagt attagattta ctcaaaatca
       61 aggattttga agatgaactt gtatcaaaaa atcgcggttt aaaatcgcga ttttttataa
      121 tttctcgtta acaaagcgga caaactgatt ccaccaaact tttaagaaga aggctttttc
      181 aattttcttg tctgctacca tttcgaaact agggcgctct gtggtgatgt aaccttgacc
      241 aatcaagtcc ttgtcttcat aagtcaaatg gccaaccact gttccagctt caagtggtgc
      301 tgggattgct ttggaatcag gtgtgaattg aacagattgg gaagattgat tcccaacacg
      361 ttcgattaga tagatatcct ctggagccac tgcagttact gtatcttctt ttccatcttg
      421 tacaggggct ttgctatctt gataggcatc gccttgttga acgattttgc gaagtgtaaa
      481 tgtagaagaa atataatcca ttagggaaga tgtagctgta aatcgagcgt aaggattatt
      541 gtcttgatga tctgcattta aaacaactgt gatgactctc atgccttttt cgacagtagt
      601 accaacaaaa gactctccag ccttatctgt tgttcctgtt tttagcccat caaaaccacc
      661 acggtaagca ggcatacctt ctaacatgta gttggttgaa gtgattgtca tcccagcaaa
      721 agtagaagaa ggttttttgg tgatttctaa gacttgtggg tattttttga tgaggttgcg
      781 agcaacgata gcgacatcat aagcactaag cttattttcc tcatcttttt tagaacctgg
      841 gtaaatgtta tcccctagag tttcattgtt aagacctgtc gtattgacaa cagtggcatc
      901 ctgaattccc cattccaaga gttttgcccg catcatatcg atgaaatctt tttctgagcc
      961 agcaattttc tcagctaggg caatagcggc gctgttggca ctagatacca gagttgcttc
     1021 aagcaactct tcgacagtat aattacgggc ctccatagga atattactgg cttcagaatt
     1081 tgtcgtcaat tgataaggat aatcagaaat atctacagga gtggagaggg taatacttcc
     1141 gttttccaaa gcttcataga ccagataagc agtaatcaat tttgttatgg aagcaatttc
     1201 gacaggttgc gttgcatcct tctcatagag aattttacca gtatttgcct caacagcaat
     1261 cgcatgttta gcggcaatgg taaaatcttg agcaacagca gtagaagcac cccctaaaag
     1321 agagacagtt aacaaagtta aaaatatttt tttcatagta gtcttattct atcataaaga
     1381 aaaaaaatat tcttgcttta ataattcatc tgttaagctt tttgaaaata tggtaaaata
     1441 aagtgaggga ggtaactcat gtttcgtaga aataaattat ttttttggac cacagaaatt
     1501 ttactcttaa ccatcatctt ttacctatgg agacagatgg ggtctttgat taaccctttt
     1561 gttagcgtgc ttaatacaat tatgattcca tttttattag ggggctttct ttattatttg
     1621 acaaacccta ttgttacttt cttaaataaa gtctgtaaac tcaatcgttt gcttggtatt
     1681 ttaattacct tgtgtacttt ggtctgggga atggtcatag gtgttgtcta tctcttacct
     1741 attttgatta atcagttatc tagtttgatt atatctagtc aaactattta tagtcgagta
     1801 caagacttaa tcatagactt atctaattat cctgcgctcc agaatttgga tgtagaagct
     1861 acaattcagc agttaaactt atcctatgtt gatattcttc aaaatatcct aaatagcgta
     1921 tcaaatagtg tggggagcgt cttgtcagct cttatcagta ctgttttgat tttgattatg
     1981 actccagttt ttttggttta tttcttatta gatggacata aattcttgcc catgcttgaa
     2041 agaacgattc taaagaggga tcgcttgcat attgcaggct tattaaagaa tttaaatgcg
     2101 acgattgctc gctatattag tggagtttcg attgacgcaa tcattatagg ttgtttggct
     2161 tatattggct atagtattat tggtttaaaa tatgctttag tttttgccat tttttctggt
     2221 gtagccaatt taattcctta tgtggggcca agtattggtt tgattcctat gatcatcgca
     2281 aatatattca ctgatcccca tagactgctg attgcagtga tttatatgct tgttgttcag
     2341 caggtagatg gcaatatctt atatcctcga atcgtaggaa gtgttatgaa ggttcatcca
     2401 atcacgattt tagttttact tttgttgtca agcaatatct atggtgtagt tggaatgatt
     2461 gtcgcagtgc caacctattc tatcttgaaa gaaatttcta agttcttatc ccgtttgtat
     2521 gaaaatcata aaataatgaa agaacgagaa agagaattag ctaagtaaaa gtcaggagaa
     2581 ccctgatttt tctttactgg aagtggcctt tagattagaa gactgaaaat aagttaaagt
     2641 cttaaactaa ttttcacagc taagaatagt agaagttaat ctgataaaaa tcgaaaaaac
     2701 cagtggaatt ctgtgtcagg gtaagttcca ctggttttca tagtctatta aagttcgaat
     2761 gaaacctatt tatagtagat tgaaactaga atagtacacc tctaattcta aaacattgtt
     2821 agaaatcgat ttgactgtcc tgatctattc gttctattct tatttcattt tactatattt
     2881 tggtgcaata agtgaaaagt agtccgaata atataaggat tgattttata gtttttaaac
     2941 tcaaatgaat tgaaataaag agagtacgaa aattctcatc tgaaagtatt ttagaataat
     3001 tctcttcgtg aatttcttca aaacagatag cttcatctta ggtatgtgat ttctttttgc
     3061 atttttgagt tagataaggt ataatggttt tattgtcttt tggggtcgtt acggattcga
     3121 caggcattat gaggcatatt ttgcgactcg tgtggcgacg taaacgctca gttaaatata
     3181 actgcaaaaa ataacacttc ttacgctcta gctgcctaaa aaccagcagg cgtgacccga
     3241 tttggattgc tcgtgttcaa tgacaggtct tattattagc gagatacgat taagccttgt
     3301 ctagcggttt gataagagat tgatagactc gcagtttcta gacttgagtt atgtgtcgag
     3361 gggctgttaa aataatacat aacctatggt tgtagacaaa tatgttggca ggtgtttgga
     3421 cgtgggttcg actcccaccg gctccattat tcctttgcat tcttttgcat tccttggtaa
     3481 aacgttgtta aatcaacgtt ttttattttt atctttggta ttcctttgca ttcttttgct
     3541 aaaaagggag tcacaaacag accctatttt aaaaaaagga tagaaaaaag gatacaacat
     3601 ttgtcgcatc ctaaaaataa tcttttttcc acggaagaca tgggattcga acccacgcac
     3661 gctattacac gcctaccgcg tttccaacac ggcctcttaa gcctcttgag taatcttcca
     3721 atacttactc aaatagtcta ccataaaggc tcttatcttg caataaaaat tctagaaata
     3781 agaaaaatga tagattttga aagaaaatga taaaaaatgc ttgacttcaa aagaaagtat
     3841 gatagaatga atagtgtaaa cgataacagg aggtgattcg gtgttaaaaa cagagcggaa
     3901 gcaactgatt ttagaggagt taaatcaaca tcatgtagtt tctctagaaa aattagttag
     3961 tttgctagaa acgtcagaat caacggttcg aagagacttg gatgagttgg aagcggaaaa
     4021 caagcttcgt cgtgtgcatg gtggagcaga actcccccac tccttacagg aagaagaaac
     4081 cattcaagaa aaatctgtca aaaaccttca agaaaagaag ttgctggctc agaaagcagc
     4141 ctctctcatt aaagaaaaag atgtcatctt tatcgatgct ggaacaacaa ctgctttttt
     4201 gattcatgaa ttggtcaata agaatattac agttgtgacc aactccattc accatgccgc
     4261 tcagttggtt gaaaagcaga ttccaactgt catggttgga ggaaacgtca agacggcgac
     4321 agatgctagt atcggtggcg ttgctcttaa ccagattaac caattgcact ttgaccgtgc
     4381 ctttatcgga atgaatggtg ttgacgatgg ctattatacg actcctgata tggaggaggg
     4441 agctgtgaaa agagctattt tggagaatgc caagcagacc tacgtcttgg tggattcgtc
     4501 aaaaattgga caaacttgct ttgccaaggt agcaccactc aaacgcgcta tcgttatcac
     4561 tagtcaaggg catgagctct tgcaggttat taaggagaaa acggaggtaa tagaagtatg
     4621 atttatacag tcacactcaa tccatccatt gactatatcg ttcgtttgga ccaagtcaaa
     4681 gttggtagtg taaatcgtat ggacagtgat gataagtttg ctggtgggaa aggaatcaat
     4741 gtcagccgtg tcttgaaacg tttgaatata ccaaatacag cgacgggatt tatcggtggc
     4801 tttactggta aatttatcac agatacttta gcagaggaag aaatcgagac acgttttgtc
     4861 caggtggcag aagatactcg tatcaatgtt aaaatcaaag cagaccaaga aacagaaatc
     4921 aacggaacgg gtccaactgt tgaatcggtt cagctagaag aattgaaagc tattttatct
     4981 agtctgacag cagaagatac agttgtcttt gcaggttcaa gtgctaaaaa tctaggcaat
     5041 gttatctata aggatttgat ttccttgacg cgccagactg gtgcgcaagt ggtctgtgac
     5101 tttgaaggac agaccttaat tgatagtttg gactaccagc ctcttcttgt aaaaccaaac
     5161 aatcatgaac ttggagcgat ttttggggtt aaactcgaaa gtttagatga aattgagaaa
     5221 tacgctcgtg agttactggc taagggtgct caaaatgtta ttatctctat ggctggtgat
     5281 ggtgcccttc ttgtcacatc tgagggagct tacttcgcta aaccaatcaa aggaacagtc
     5341 aaaaattcag ttggagctgg tgattctatg gttgctggat tcacaggtga atttgtcaaa
     5401 tcaaaagacg tagtagaagc cttcaaatgg ggagtggctt gcggaacggc aactaccttc
     5461 tcagatgact tggcaacggc ggaatttatt aaagaaacat atggaaaagt tgaggtagaa
     5521 aaacgatgaa aattcaagac ctattgagaa aagatgtcat gttgctagat ttgcaggcaa
     5581 ctgaaaaaac agctgtcatc gacgagatga ttaaaaattt gacagaccac ggttatgtaa
     5641 cagattttga aacatttaaa gaaggaattt tggcgcgtga agctttgact tctactggtt
     5701 tgggtgatgg aatcgcaatg cctcacagca aaaacgctgc tgtcaaagaa gcgacagttc
     5761 tatttgctaa gtcaaataag ggtgttgact acgagagctt ggatggacaa gcaactgacc
     5821 tcttcttcat gattgcagct ccagaaggtg ccaatgatac tcacttggca gccttggcag
     5881 aattgtctca atacttgatg aaagacggtt ttgcagacaa acttcgtcaa gcaacatctg
     5941 cagaccaagt tatcgaactt tttgaccaag cttcagaaaa aactgaggaa cttgttcaag
     6001 cacctgctaa tgactctggt gactttatcg tagctgttac agcttgtaca acaggtattg
     6061 cccacactta catggcccaa gaagcccttc aaaaagtagc tgctgaaatg ggggttggta
     6121 tcaaggtcga aaccaacggt gctagcggtg ttggaaatca actaactgca gaagatatcc
     6181 gtaaggctaa agctattatc attgcagcag acaaggccgt tgaaatggat cgatttgatg
     6241 gaaaaccatt gatcaatcgt ccagttgctg acggtatccg taagacagaa gagctaatta
     6301 acttggctct ttcaggagat actgaagtct accgtgccgc taatggtgcc aaagctgcaa
     6361 cagcctctaa cgaaaaacaa agccttggtg gtgccttgta caaacacttg atgagtggtg
     6421 tatctcaaat gttaccattc gttatcggtg gtggtatcat gattgccctt gccttcttga
     6481 ttgacggtgc tttgggtgtt ccaaatgaaa accttggcaa tcttggttct taccatgagt
     6541 tagcttctat gttcatgaaa attggtggag ctgcctttgg tttgatgctt ccagtctttg
     6601 cgggttatgt tgcctactct attgctgaaa aaccgggttt ggtagcaggt ttcgtggctg
     6661 gtgctattgc caaagaaggt tttgcctttg gtaaaattcc ttatgccgca ggtggtgaag
     6721 caacttcaac tcttgcaggt gtctcatctg gtttcctagg tgcccttgtt ggtggattta
     6781 tcgcaggtgc cttggttctt gccatcaaga aatacgttaa agttcctcgt tcactcgaag
     6841 gtgctaaatc aatccttcta ttgccacttc ttggaacaat cttgacagga tttgttatgc
     6901 tagctgtgaa tatcccaatg gctgcaatca acactgctat gaatgacttc ctaggcggtc
     6961 ttggaggagg ttcagctgtc cttcttggta tcgtccttgg tggaatgatg gctgttgaca
     7021 tgggtggacc agttaataaa gcagcttatg tctttggtac aggtacgctt gcagcaactg
     7081 tttcttcagg tggttctgta gccatggcag cagttatggc tggaggaatg gtgccaccac
     7141 ttgcaatctt tgtcgcaact cttcttttca aagataaatt tactaaggaa gaacgtaact
     7201 ctggtttgac aaacatcatc atgggcttgt catttatcac tgagggagcg attccatttg
     7261 gtgccgctga cccagctcgt gcgattccaa gcttcatcct tggttcagca gtagcaggtg
     7321 gactcgttgg tcttactggt atcaaactca tggcgccaca cggaggaatc ttcgttatcg
     7381 cccttacttc aaatgctctc ctttacctcg tttctgtctt ggtaggagca atcgtaagtg
     7441 gtgtggttta tggttaccta cgcaaaccac aagcataaaa aatagaaaaa tgaaaagatt
     7501 ggaccgtttg gtgcagtctt tttctcttcc cgaaatgcct gtgaaatatg gtataataga
     7561 agaatggcaa acaagaatac aagtacaaca agacggagac cgtctaaagc agaactggaa
     7621 agaaaagaag cgattcaacg aatgttgatt tcgttaggaa ttgcgatttt attgattttc
     7681 gcagccttca aattaggggc tgcaggtata accctttata atttaattcg cttgctagtg
     7741 ggtagcctag cttatctggc gatattcggc ctattaatct atctcttctt tttcaagtgg
     7801 atacgaaaac aggaaggact cttatctggc tttttcacca tatttgctgg cttactcttg
     7861 atttttgagg cctacttggt ttggaaatat ggtttggaca agtccgttct aaaagggacc
     7921 atggctcagg ttgtgacaga tctgactggt tttcgaacga ctagctttgc tggagggggc
     7981 ttgatcgggg tcgctcttta tattccaaca gcctttctct tttcaaatat cggaacttac
     8041 tttattggtt ctatcttgat tttagtgggt tctctcctag tcagcccttg gtctgtttac
     8101 gatattgctg aatttttcag tagaggcttt gccaaatggt gggaagggca cgagcgtcga
     8161 aaagaggaac gctttgtcaa acaagaagaa aaagctcgcc aaaaggctga gaaagaggct
     8221 agattagaac aagaagagac tgaaaaagcc ttactcgatt tgcctcctgt tgatatggaa
     8281 acgggtgaaa ttctgacaga ggaagctgtt caaaatcttc cacctattcc agaagaaaag
     8341 tgggtggaac cagaaatcat cctgcctcaa gctgaactta aattccctga acaggaagat
     8401 gactcagatg acgaagatgt tcaggtcgat ttttcagcca aagaagccct tgaatacaaa
     8461 cttccaagct tacaactctt tgcaccagat aaaccaaaag atcagtctaa agagaagaaa
     8521 attgtcagag aaaatatcaa aatcttagaa gcaacctttg ctagctttgg tattaaggta
     8581 acagttgaac gggccgaaat tgggccatca gtgaccaagt atgaagtcaa gccggctgtt
     8641 ggtgtaaggg tcaaccgcat ttccaatcta tcagatgacc tcgctctagc cttggctgcc
     8701 aaagatgtcc ggattgaagc accaatccct gggaaatccc taatcggaat tgaagtgccc
     8761 aactccgata ttgccactgt atctttccga gaactatggg aacaatcgca aacgaaagca
     8821 gaaaatttct tggaaattcc tttagggaag gctgttaatg gaaccgcaag agcttttgac
     8881 ctttctaaaa tgccccactt gctagttgca ggttcaacgg gttcagggaa gtcagtagca
     8941 gttaacggca ttattgctag cattctcatg aaggcgagac cagatcaagt taaatttatg
     9001 atggtcgatc ccaagatggt tgagttatct gtttacaatg atattcccca cctcttgatt
     9061 ccagtcgtga ccaatccacg caaagccagc aaggctctgc aaaaggttgt ggatgaaatg
     9121 gaaaaccgtt atgaactctt tgccaaggtg ggagttcgga atattgcagg ttttaatgcc
     9181 aaggtagaag agttcaattc ccagtctgag tacaagcaaa ttccgctacc attcattgtc
     9241 gtgattgtgg atgagttggc tgacctcatg atggtggcca gcaaggaagt ggaagatgct
     9301 atcatccgtc ttgggcagaa ggcgcgtgct gcaggtatcc acatgattct tgcaactcag
     9361 cgtccatctg ttgatgtcat ctctggtttg attaaggcca atgttccatc tcgtgtagca
     9421 tttgcggttt catcaggaac agactcccgt acgattttgg atgaaaatgg agcagaaaaa
     9481 cttcttggtc gaggagacat gctctttaaa ccgattgatg aaaatcatcc agttcgtctc
     9541 caaggctcct ttatctcgga tgacgatgtt gagcgcattg tgaacttcat caagactcag
     9601 gcagatgcag actacgatga gagttttgat ccaggtgagg tttctgaaaa tgaaggagaa
     9661 ttttcggatg gagatgctgg tggtgatccg ctttttgaag aagctaagtc tttggttatc
     9721 gaaacacaga aagccagtgc gtctatgatt cagcgtcgtt tatcagttgg atttaaccgt
     9781 gcgacccgtc tcatggaaga actggagata gcaggtgtca tcggtccagc tgaaggtacc
     9841 aaacctcgaa aagtgttaca acaataaaaa aatagcttct ttccaagttt ggagggaagc
     9901 tattttagtg gctattgatt gcttttattt tctgaagttg gcgcattgga ctgtttttcg
     9961 ttttcagtag caggtttact tgaagcagga gtagaagagt cctgagttgc tgttttctga
    10021 tcttcttttt tctcttcctt gacgctagat tttggtgttt cctcttgctg tgttttttct
    10081 tgactagtgt tagtctcttt agttggactg gtgttttcct taggggattc cttttggatt
    10141 tctttgacaa tggttgtcgt ctggcttgtc gtaggttctt ttttaatatt tttgttatta
    10201 tccaaggcgt tcataatcgt gatacttgcg gtaatcaagc caaggatact agcgattgta
    10261 gcaacggttt tttgaaagac tgtaaagcct tttttgatgt gttctgtttt tggttttgaa
    10321 gttctacgat agttagacat acattctctc ctttgattat gatttattat actctgtttc
    10381 tttaaaaaaa tcttaaaaaa agagaaattt ccctttcttg tcgcaagctc cgtttcatga
    10441 tacaatagag gaagtgattt tagaaagcgt gagaaacatg atttactttg ataattcagc
    10501 gacgaccaag ccttatcctg aagcccttga aacctatatg caggtcgctt caaaaatttt
    10561 aggaaaccca tctagtctcc atcgtttggg agaccaagca acacgaatct tagatgcttc
    10621 tcgccaacag attgcagatt taatcggtaa gaaaagcgat gaaatcttct ttacctcggg
    10681 tggaacagaa ggggataact ggcttatcaa gggtgtggcc tttgaaaaag ctcagtttgg
    10741 caagcacatc attgtttcag ccattgaaca tccagcagtc aaagagtcag ccctctggtt
    10801 gaaaagtcaa ggatttgaag tggattttgc tccagttgat aagaaaggct tggtcgatgt
    10861 tgaggcgtta gcaggtttga tacggcatga tacaatcctc gtttccatca tggctgtgaa
    10921 caatgaaatc ggctctatcc aacctattga ggctatttca gaattcttgg cagacaagcc
    10981 gactatttcc ttccacgttg atgcggttca ggcgcttgcc aaaattccga ctgaaaagta
    11041 tctgacagaa cgggtggatt gcgcgacttt ctctagtcac aagttccacg gggttcgagg
    11101 tgttggcttt gtctatatca aatctggcaa gaagattaca cctcttctta caggtggtgg
    11161 ccaggagcga gattatcgtt cgacaactga aaatgtggca gggattgcag cgacagccaa
    11221 ggccctccgt ttgtctatgg aaaagctaga tatctttagg agcaagactg ggcagatgaa
    11281 ggcagtgatt cgccaagctc ttctgaacta tccggatatt tttgtctttt cagatgagga
    11341 aaactttgca cctcatattc tgacttttgg aatcaaaggt gttcgaggtg aagtcatcgt
    11401 tcacgccttt gaagactatg atattttcat ctcaacaacc tcagcttgtt catctaaggc
    11461 aggaaaacca gccggtacct tgattgccat gggagtggac aaagataagg ccaagtcagc
    11521 tgtgcgtctt agcctagact tggaaaatga tatgagtcag gtcgagcagt ttttgaccaa
    11581 gttaaaattg atttacaatc aaactagaaa agtaagatag gagcattcat gcagtattca
    11641 gaaattatga ttcgctacgg agagttgtca accaagggta aaaaccgtat gcgtttcatc
    11701 aataaacttc gtaataatat ttcggacgtt ttgtctatct atacccaagt taaggtaaca
    11761 gcagatcgcg accgtgccca cgcttacctc aatggagctg attacacagc agttgcagaa
    11821 tctctcaaac aagtttttgg aattcaaaac ttttctcctg tttataaggt tgaaaaatct
    11881 gtagaagttt tgaagtcttc tgtccaagag attatgcggg acatctacaa ggaaggtatg
    11941 acctttaaga tttctagcaa gcgtagcgac cacaactttg aacttgatag tcgtgaactc
    12001 aaccaaacac ttggaggggc tgtattcgaa gccattccaa atgtgcaagt tcaaatgaaa
    12061 agtcctgaca tcaatcttca ggtggagatt cgtgaagaag cagcctatct ttcttatgaa
    12121 accattcgtg gggctggtgg tttgccagtt ggaacttcag gtaaagggat gctcatgttg
    12181 tcaggaggga ttgactcacc tgtagcaggt tatcttgctc ttaagcgtgg ggtggatatc
    12241 gaggcagttc actttgctag tccaccatat actagtcctg gtgccctcaa gaaagcgcag
    12301 gacttgaccc gtaaattgac caagtttggc ggaaatatcc agtttataga ggtgcctttc
    12361 acagagattc aagaggaaat caaagccaaa gcgccagaag cttatttgat gactctaact
    12421 cgtcgcttta tgatgcggat tactgaccgt attcgtgagg tacgaaatgg tttggttatc
    12481 atcaatgggg aaagtctagg tcaagtagcc agccaaaccc ttgaaagtat gaaggctatc
    12541 aatgctgtta ccaacactcc catcattcgt cctgtggtta ccatggacaa gttggaaatc
    12601 attgacatcg cccaggaaat cgataccttt gacatttcaa tccaaccgtt tgaagactgt
    12661 tgtaccattt ttgcaccaga tcgtccaaaa acaaatccta aaattaagaa tgcggagcag
    12721 tacgaagcgc gtatggatgt tgaaggcttg gttgagcgag cagtggctgg aatcatgatt
    12781 actgaaatca cacctcaagc cgaaaaagat gaagttgatg acttgattga caatctgctc
    12841 taattcagaa aatccaaaag aatagcgaaa atcagtaaaa aaagttagtt ttttctctaa
    12901 aaacaggtaa aaaactaact ttttttattt ttatgatata atgatataaa attttgaata
    12961 tagagagttt tctgacaatg aatcaatcct acttttatct aaaaatgaaa gaacacaaac
    13021 tcaaggttcc ttatacaggt aaggagcgcc gtgtacgtat tcttcttcct aaagattatg
    13081 agaaagatac agaccgttcc tatcctgttg tatactttca tgacgggcaa aatgttttta
    13141 atagcaaaga gtctttcatt ggacattcat ggaagattat cccagctatc aaacgaaatc
    13201 cggatatcag tcgcatgatt gtcgttgcta ttgacaatga tggtatgggg cggatgaatg
    13261 agtatgcggc ttggaagttc caagaatctc ctatcccagg gcagcagttt ggtggtaagg
    13321 gtgtggagta tgctgagttt gtcatggagg tggtcaagcc ttttatcgat gagacctatc
    13381 gtacaaaagc agactgccag catacggcta tgattggttc ctcactagga ggcaatatta
    13441 cccagtttat cggtttggaa taccaagacc aaattggttg cttgggcgtt ttttcatctg
    13501 caaactggct ccaccaagaa gcctttaacc gctatttcga gtgccagaaa ctatcgcctg
    13561 accagcgcat cttcatctat gtaggaacag aagaagcaga tgatacagac aagaccttga
    13621 tggatggcaa tatcaaacaa gcctatatcg actcgtcgct ttgctattac catgatttga
    13681 tagcaggggg agtacatctg gataatcttg tgctaaaagt tcagtctggt gccatccata
    13741 gtgaaatccc ttggtcagaa aatctaccag attgtctgag attttttgca gaaaaatggt
    13801 aagttaagaa aggaaaaaac gaaatgcata ttgaacatct tagccactgg agtggtcatc
    13861 ttaaccgtga aatgtacctt aaccgttatg gacatggtgg gattccagtt gtggtctttg
    13921 cttcatcagg tggtagtcac aacgaatact atgattttgg catgattgat gcctgtgctt
    13981 cctttatcga ggaaggcctt gtccagttct ttaccctatc tagtttggat agtgagagct
    14041 ggttggctac ttggaaaaat gctcatgacc aagcggaaat gcaccgtgcc tacgaacgtt
    14101 atgtgattga ggaggccatt cttttatcaa gcacaagaca ggttggtttg atggcatgat
    14161 gacgacaggt tgctctatgg gagcctatca tgcactcaat ttcttcctcc agcatccaga
    14221 tgtctttacc aaagtgattg ctctcagtgg tgtttacgac gcacgtttct ttgtcggtga
    14281 ttactacaac gatgatgcta tttaccaaaa ctcgccagta gattatattt ggaaccaaaa
    14341 cgacggctgg tttattgacc gttaccgtca ggcagagatt gtgctgtgta cggggcttgg
    14401 agcctgggaa caagatggtt tgccatcctt ttacaagctc aaagaagcct ttgacaagaa
    14461 acaaattcca gcctggtttg ctgaatgggg acatgatgtc gcccatgact gggaatggtg
    14521 gcgtaaacaa atgccttatt tcctcggtaa tctctattta taaaaggagt tacctatgaa
    14581 ttaccttgtt atttctccct actatccaca aaactttcaa cagtttacca tcgaactagc
    14641 taataaaggc atcacagtct tgggaattgg tcaagagtct tacgagcaat tggatgagcc
    14701 cttgcgcaat agcttgaccg agtattttcg tgttgataat cttgagaaca tagatgaagt
    14761 caaacgtgca gttgcttttc tcttttataa acatggtcca attggccgca tcgagtctca
    14821 caatgaatac tggcttgagc tagacgcaac actcagagaa caattcaatg tttttggtgc
    14881 caaaccagag gatctcaaaa agacgaaata taagtctgaa atgaagaaac ttttcaaaaa
    14941 agcaggtgtt cctgtggtac ctggagctgt tatcaagacg gaagcagatg ttgatcaagc
    15001 agtgaaagaa atcggtcttc caatgattgc caaacctgat aatggagtgg gagcagccgc
    15061 aacctttaaa cttgagacag aagacgatat caatcacttc aagcaagaat gggaccattc
    15121 aaccctttat ttctttgaaa aatttgtcac ttccagcgaa atctgtacct ttgacgggct
    15181 cgtggacaag gatggaaaga ttgtcttctc aacaaccttt gactacgcct atacaccgct
    15241 tgacctcatg atttataaga tggacaattc ttattatgtg ctcaaggata tggatcctaa
    15301 actgcgcaag tatggggaag caattgtcaa agaatttggt atgaaagaac ggtttttcca
    15361 tattgagttc ttccgtgagg gggacgatta tattaccatc gagtacaata accgccctgc
    15421 aggtggtttt accattgatg tttataactt tgctcattcc ttggaccttt atcgtggcta
    15481 tgcagctatt gtcgcaggag aggagttccc ggcgtcagac tttgaaactc agtattgttt
    15541 ggctacttct cgccgtgcaa atgctcacta tgtttattca gaagaggatt tgcttgccaa
    15601 atatagccag cagttcaagg ttaaaaaagt catgccagct gccttcgcgg aacttcaagg
    15661 agattacctg tatatgctga ccactccgag tcgacaagaa atggagcaga tgattgcaga
    15721 tttcggacaa cgtcaagaat aagaactatc ggattaagga aattaactcc cttaatcctt
    15781 ttgttttgtc tgataaaaaa taagagcatc ccaacaaggt agctatcata aaacttgttc
    15841 gataactatt tgaagcagga ttaggtggtc agaaattaaa ttttaatatt tcaattgagt
    15901 catagtattg tgtttgcgta tccttaaatc agctaaaagg atccatgacg acacctatac
    15961 gatatagttt tcaagatacc aaacaagtct attaat
//
LOCUS       DAVBCW010000041        15200 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVBCW010000041 DAVBCW010000000
VERSION     DAVBCW010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15200
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.041"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            213..1895
                     /locus_tag="VQ514_001103"
     CDS             213..1895
                     /locus_tag="VQ514_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2499866.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQTAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLANLDNMSIS
                     KGQVQLQNELVKETPELQSLFKLEDVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHRKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            1880..2710
                     /locus_tag="VQ514_001104"
     CDS             1880..2710
                     /locus_tag="VQ514_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HET2499867.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVASLLLSLQNQGRFYNPWK
                     "
     gene            2903..3406
                     /locus_tag="VQ514_001105"
     CDS             2903..3406
                     /locus_tag="VQ514_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HET2499868.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKVYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     regulatory      3460..3694
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            3859..4422
                     /locus_tag="VQ514_001106"
     CDS             3859..4422
                     /locus_tag="VQ514_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2499869.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTIGLTVAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYILLKPTLKHYER"
     gene            4436..5062
                     /locus_tag="VQ514_001107"
     CDS             4436..5062
                     /locus_tag="VQ514_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET2499870.1"
                     /translation="MKGSFALLLFVILGYMVKFYPEMLVNFDQSIQTAIRGDLPDYLT
                     ILFRSLTRLIDIPVIITWVVIIAFVFYRKRWKIESFFMLGNLTLAGLLIVTFKNIYQR
                     PRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKDQVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVLASLCVGLGALFIEFPFYDKLRFQWRFKGKQK"
     gene            5174..6046
                     /locus_tag="VQ514_001108"
     CDS             5174..6046
                     /locus_tag="VQ514_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000525616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2499871.1"
                     /translation="MGMLLATRLKNKRKELKLSQKELAEGICEQGQISRMEQGKYSPG
                     SELLFQLSKRLKVSMDYFFEDTEVSSLENIDKFKELSKKFLDEREYESLHYIYELEKS
                     KRARLSAKDQIYLDWIDTLVQFCYLNHQTGAVAKLEKILLSLKKSDFYYLQLGNTLAN
                     FYFDMGQEDTYQELYQLLKENLQSLSVHHLEQLELMIKFRYNYCRYLWLNERTSEAIE
                     EITETIAICKRYNSSYRLADLYCLLGSISEGFAERDRILGFYQVSYQLYKHEENQRMM
                     LEIEKEMKLLESED"
     gene            6176..6313
                     /locus_tag="VQ514_001109"
     CDS             6176..6313
                     /locus_tag="VQ514_001109"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499872.1"
                     /translation="MKRSQVVKRCILIALLLLFLLNVLSGSLSVTTRGVDPRPAYGVS
                     L"
     gene            6737..7651
                     /locus_tag="VQ514_001110"
     CDS             6737..7651
                     /locus_tag="VQ514_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HET2499873.1"
                     /translation="MKLYQINDGIQIFESSNLEKIVIRNYRNGKKITIDHQPLLKKLL
                     LFLEVPKREEEILLYLPELSNKTLRSVLTNFMSLNIIHCQDTLIIHKKINILIIGLGT
                     TGGYLADGLCRSNINSKLFLLDKDRVDETNVGRQIYLRDDIGQYKVDVFKQKFSDKEI
                     KVFKIFINSKKDLEKICKREGIDLVIQCGDSPSPRELGKIVNTVCDTLQIPYIVNTGY
                     MSNVVPLPEFYYPNNRYNFNYKHQFSDEKLLFSQVLSKADYMVAIQSSFVMLMQIKNF
                     IFNEEPIYYGYRGYYNSVTLAWEVEKID"
     gene            7644..8657
                     /locus_tag="VQ514_001111"
     CDS             7644..8657
                     /locus_tag="VQ514_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499874.1"
                     /translation="MTKYLELDYSHSYILEGFRKNDAFIENHKECLDMLSDKIWRDNK
                     YMNTEKNISSPTRTILSRYTCNILSSLFIDISKNSIEKLIVTCENSDLNWIYNYYKSV
                     YNGVFCELRQLIQQYCKVKDKYEKWLFIKAIINQGIRQNEKEVVEFFLKQLKDNNQGI
                     FDYINSFSFYLLKFYSKDKSRIFLEEQLDIDDFLGSDKEDYARSLVFKNYASLLPDTS
                     ELKRNIMEKCLSQTPQDTDLWKEWFETYASQKEIRQKATEIFKHGYSDISLLKLVKIE
                     PDDTESLIRMIILCTSDLNSNIARYLISFLSDGRLKEFLELFVENFDFLNIIEGKTSE
                     INF"
     gene            8641..10228
                     /locus_tag="VQ514_001112"
                     /pseudo
     CDS             8641..10228
                     /locus_tag="VQ514_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236730.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            10420..11323
                     /locus_tag="VQ514_001113"
                     /pseudo
     CDS             10420..11323
                     /locus_tag="VQ514_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091364.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            11341..11478
                     /locus_tag="VQ514_001114"
                     /pseudo
     CDS             11341..11478
                     /locus_tag="VQ514_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
     gene            11506..11847
                     /locus_tag="VQ514_001115"
     CDS             11506..11847
                     /locus_tag="VQ514_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499875.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLKNHI
                     LPLWIGMVSFF"
     gene            11820..12131
                     /locus_tag="VQ514_001116"
                     /pseudo
     CDS             11820..12131
                     /locus_tag="VQ514_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            12259..12846
                     /locus_tag="VQ514_001117"
     CDS             12259..12846
                     /locus_tag="VQ514_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HET2499876.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            13236..14843
                     /locus_tag="VQ514_001118"
     CDS             13236..14843
                     /locus_tag="VQ514_001118"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET2499877.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDVEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
BASE COUNT         4705 a         2370 c         3205 g         4920 t
ORIGIN      
        1 aaggcgaaac tgacgtggtt tgaagagatt ttcgaagagt ataaaatgat ggggaaggct
       61 gggcaactag tctttttcga tgtttataga ttagttaggc aagtaagcta ggataggaat
      121 ctttttggtg ctaagatttt aagagaagag actgcaagaa gggcttgatt tatgataaaa
      181 tagaagtcta agaagcgaat gaagagagta agatgaaaga agctataatt gagtggaagg
      241 atttctcttt ccggtatgaa acacaacaag aaccgacctt gcaagggata gacttgacca
      301 tttacaaggg agagaaagtc ttaattgttg gaccatctgg atcaggtaaa tctaccttgg
      361 gtcagtgttt gaatgggatt attcccaata tttacaaggg tcagacatat ggagaatttt
      421 tgataaaggg tcaaacagcc tttgatatga gcatctatga taagtctcat ctggttagca
      481 cagttttgca ggatacagat gggcagttta ttggcttgtc tgtggcagaa gatttggcgt
      541 ttgctctgga aaatgatgtg acagccctag atgagatgaa aggtcgtgtt tataaatggg
      601 ctgaaaagct ggaccttctt cctttactgg atcagcgtcc tcaggatttg tcaggtggac
      661 aaaagcagcg agtcagtctg gctggtgtct tgattgatga aagtccgatt ctcttgtttg
      721 atgagccact cgccaatcta gatcccaagt caggtcagga tattatcgaa ttgattgacc
      781 agattcataa ggaagagggg acgacgactc ttattatcga gcaccgtttg gaggatgttc
      841 tgcatcgccc tgtggatcgg attgtcttga taaacgatgg tcgtatcctt tttaatggga
      901 gccctgacca gttgcttgcg actgatttat tgactcaaaa tggaattcga gaaccccttt
      961 atctaaccac tctccgtcaa ttaggtgtgg acttagtcaa ggaagaacag ttagcgaatc
     1021 tggataacat gtctatctca aaaggtcagg ttcagctgca gaatgaactg gtaaaagaaa
     1081 ctccagaatt gcagtcactc tttaaattag aggacgtgtc tttctcttat gatgatagac
     1141 cgattttaaa atccctacat ttagatatta aaaagggtga aaagattgct attgtcggaa
     1201 aaaatggagc agggaaatca actctagcca aggctataag tagctttatt cagacggaag
     1261 gacgctatct ttgggaaaaa caggatataa aaggcgattc tgttgcagag cgggcggaac
     1321 gagtaggcta tgtgctacaa aatcctaatc aaatgatttc aaccaatatg atttttgatg
     1381 aggtggctct agggctccgt ttgcgaggtg tggatgagaa ggaaattgaa acgagagtct
     1441 atgaaacctt gaaaatctgt ggactttatg aattccgtaa ttggcctatt tctgccctgt
     1501 catttggtca gaaaaaacgt gtcaccattg cttcaatttt ggtcttagga gctgaaatta
     1561 ttctcctaga tgaaccgact gcaggtcaag atcagaagaa ctatactgag attatggaat
     1621 ttctcgaaga gttacatcga aaagggcata ccattgtcat gattacccat gatatgcaat
     1681 tgatgctgga ttattcagac cgagtccttg tcatggtgga tggagaattg attgccgata
     1741 ctgttccagc cagtctgttg agcgatcctg agctgttagt aaaagccaat ctaaaagaaa
     1801 cctccatctt taacttggct aagaaactag atgtggatcc actggattta acggcatttt
     1861 acaaagaaag gagagaggga tgcaagctaa attaatcggt taccagcata gagatactgt
     1921 gattcatcgc ttgtcaggag ctgggaaact tctctttttc attctggtat cattggcggc
     1981 catgattagc tatgatacca gaatgctctt gctgattgcc atcttttcag tctttctcct
     2041 ctatttatca gaaattcgct ttaaagatgt ttcctttgta gccgtttttg cgacggtatt
     2101 tgccgtttta aacgttttga tggtctatct cttttctccc gagtatgggg ttggacttta
     2161 tggagagaga agtgtgattt ggcagggaat cggtgtctac actctaacca gccaggagct
     2221 cttttatctg ctaaatttgg ccattaagta tctttgcacc attcctctgg ctattatctt
     2281 tttgatgaca acccatccta gtcagtttgc ttccagttta aatcaaattg gtgtgcccta
     2341 taagattgct tattctgtca gcctgacctt gcgctatatt ccagatttgc aggaagaatt
     2401 ctttactatc aagatgtctc aggaggcgcg tgggatggaa ttatccaaga aagcttctct
     2461 tatgcaacga atcaaaggca atctgctcat tattacgccc ttgattttta gctcgctaga
     2521 acgcattgat accattgcga ctgccatgga gcttcgccgc tttgggaaag aaaaaaaacg
     2581 cacatggtat agttatcagg ccttgaaaaa aggagactat attaccttgc tcttggcaac
     2641 cttgttttta gtagctagtt tactacttag cttgcagaat cagggacgat tttacaaccc
     2701 ttggaaatag cttgaaaaaa ttgaaaaaat caagtcgttt ctattgacaa tgattctgaa
     2761 agtgttatac taagaaagta gtttcgctga tttacttcaa acctgttgtg aggtaagtta
     2821 acgatgcctt aaccacgctg tttgctgagc ttgactccgg gcagtgtggc tatttttttg
     2881 caatggtgaa aggaagcaag tcatgacaaa tcacattgta ttatttgaac ctcaaattcc
     2941 acaaaatacg ggcaatatcg cgcgtacttg cgctgcgacc aattctcccc tccacatcat
     3001 caagcctatg ggctttccta ttgatgaccg caagatgaag cgggctggat tggattactg
     3061 ggataagcta gagatttatt tttacgagag tttggaagat ttcatatctc agatgaaggg
     3121 aaaactctat ctgatttcga aattcgcgga aaaagtctat tctgaggtgg atttatcgac
     3181 tgacgaggac cattattttc tctttggacg tgaagacaag ggcttacctg aagattttat
     3241 gcgagaacat cctgagaagg ctctccgtat tcccatgaat gatgaacatg tccgcagtct
     3301 caatgtgtct aataccgtct gcatgattgt ctatgaggct cttcgccagc agaactttgc
     3361 aggtcttgaa ctcgtccata cctatgaagt ggataaattg aaataaaaaa tgaaaatgaa
     3421 caaaatgctt gcgcttgcaa gcgttttttg ttatgataaa agagtcttca gggcagggtg
     3481 tgattcccga ccggcggtga ctttaactag gaaatgatct tttcctttta tactttgttg
     3541 acaagctttg cctaaccaga agttatgcct acagcttgtc gcctagtcta aaagaaaaat
     3601 ctctatttcc ttctctttaa agaagtccgc gagcgcaagc tgatgtggtg agattccaca
     3661 accgacagta tagtctggat gggagaagac gaaagaatag ctttgtctgt tttgatagat
     3721 ttatagccgg attgtaacca cttgctttgt ttttctttaa tagagtagga gggaactttt
     3781 gggatataaa aagtgagaat agatagagga atcctttcca acttcttctg attttataga
     3841 aaattggagg aacctgttat gacaaacaca cgtcgacttt cgaccattgc aattttatca
     3901 gccatctcat ttgtgctgat gtactttgac tttccgcttt taccagcggc atctttcctc
     3961 aagatcgaat ttagtatctt gccagtcctt gtgggtctgg tggtcatgga tttgcctgct
     4021 gctctaggag tgctcttgct tcgctcactc ttgaaattgc ttcttaacag ccaaggagtg
     4081 aatacttaca ttggtttgcc gatgaatatc gtagctttgg gagtttttgt catcgtattt
     4141 gctttgattt ggaaaaagga acggacaacc cttcgtttcc tactaggctc tctagctggg
     4201 actattggtt taaccgtggc tatgttagtt ctcaactatg tttatgctgt tcctttgtac
     4261 gctaagtttg ctaactttga tattggaaaa attttgggac tttccaacta cctaatgacc
     4321 atggtattac cttttaactt gattgaaggt gtaatctttt ccgtttcatt ctggttgttg
     4381 tacattctct tgaaaccaac cttaaaacat tatgaaagat aaacaaacat ttttaatgaa
     4441 gggcagtttt gcccttttac ttttcgttat tcttggctat atggtcaaat tttaccctga
     4501 aatgttggtc aattttgacc aatcgattca gactgccatt cgaggagact taccagatta
     4561 tttgactatt ctttttcgat ccctcacacg tctgattgat attccagtga ttatcacttg
     4621 ggttgtcatt atagcttttg tcttttatcg taagcgatgg aagatagaaa gtttcttcat
     4681 gctgggaaat ctgactttgg caggtctttt aatcgtgacc tttaaaaata tctaccagcg
     4741 cccacgacca gctatcttac atctggtgga ggagaaggga ttttccttcc caagtgggca
     4801 ttctctggct gtaacgctga tggtagggac tttgattgtt attcttagtc agcggattaa
     4861 agatcaggtc tggagaaaaa tcgtacaaat cgtccttggc ctctacctag tcagtgtgct
     4921 ggtatcaaga gtctatctgg gagttcatta cccatcagat gttcttgcca gtctctgtgt
     4981 gggcttggga gccctgttta tcgagtttcc cttttatgat aagctccgct tccaatggcg
     5041 atttaaaggc aaacagaaat gatggaaaag ataaaaaatt gtacaaatac aaatagaacc
     5101 atatgaaata tatacttgaa acatgttttg gagtttgata tattttaata aataaagcaa
     5161 ggaggaagaa agtgtgggta tgttattagc gacacgctta aaaaataaaa gaaaggaatt
     5221 aaaattatct caaaaagaat tagctgaggg gatttgtgag caagggcaga ttagccgtat
     5281 ggagcaaggt aaatactcac ctgggtcaga attactcttt caactatcta agcgattgaa
     5341 ggtgagcatg gattatttct ttgaagatac tgaggtttct agtttagaaa atattgataa
     5401 gtttaaagaa ttatctaaga aatttttgga tgagagggaa tatgaatctc tccattacat
     5461 ctatgaactg gaaaaatcta aacgtgctcg tctatctgct aaagaccaga tttatttgga
     5521 ttggattgat acattagttc aattctgtta tttaaatcat caaacagggg cagttgcgaa
     5581 actggaaaaa atactgttat ctctgaaaaa atctgatttt tactatctac aactgggaaa
     5641 taccttggct aatttttatt ttgatatggg gcaggaagac acatatcagg aactctatca
     5701 acttttaaaa gagaacctcc agtcactatc tgttcatcat ttggaacagt tagaattgat
     5761 gattaaattc cgatataact attgtagata tttgtggttg aatgaacgaa catctgaagc
     5821 tatagaagaa attacagaaa cgatagctat ttgtaaaaga tataatagta gttatcgatt
     5881 agcagatttg tattgtttat tgggaagtat aagcgagggc tttgctgaaa gggataggat
     5941 actaggtttt tatcaagtat cttaccagct ttacaagcac gaagagaacc agagaatgat
     6001 gctagaaatt gagaaggaaa tgaaattatt ggaaagtgaa gattaagcaa aaagatttgt
     6061 attcttagat aactttaata catattataa aaaaaataaa aaaatatcat aaaaattatt
     6121 gacaaaccaa aattgatgtg ttagaatatc accaatagtt aaggaggttt agaagatgaa
     6181 aagatctcaa gttgtcaaac gatgtatatt aattgcttta ttgcttctat ttctacttaa
     6241 tgtgttaagt ggttctttat ctgtaacaac tagaggagtt gatccaagac cagcatatgg
     6301 agtttcgctt taaagttgtg catagttctt cttttataga cggagagttt ttctcaatag
     6361 taagtgattc aaattttgat ggatgatttt ccattaaatt aagtgattgc gaaaactttt
     6421 tgaaatacat tctctttgga tattgaaagt ttgttttgga tttttttatg aagcttatct
     6481 tagctgttag attggggttt tatgggattt gtcctcaccg ggaaaaaatc ttaaatcttt
     6541 gaagaaagag gtttttatct tatggttaga gatacatcag aagaaattga agttattgac
     6601 ttgtcttcta tgaccgtttc ctactcatct gttatgctag gaaactcttg gatgacgtgg
     6661 aattgattta ttaagataga ttatttggtt tttaccaaaa atctgtcttt ttaattttta
     6721 aaggactaat ggtgatatga aactatatca aataaatgat gggatacaaa tttttgaaag
     6781 tagtaatctt gaaaaaatag taattagaaa ttataggaat ggtaagaaaa taactatcga
     6841 tcaccaacca ttattaaaaa aattactact attccttgaa gttcctaaac gtgaagaaga
     6901 aattctatta tatcttccag aattatcaaa taaaacatta cgaagtgttt tgacgaattt
     6961 tatgagttta aatataattc attgtcaaga tacattaata attcacaaaa aaataaacat
     7021 attgataata ggtttaggga ctacgggtgg atatctggca gatgggttat gtcgtagtaa
     7081 tattaattcg aagctatttc ttttagataa ggatcgtgta gatgagacta atgtaggtag
     7141 acagatttat ttaagagatg atattggtca atataaagtt gatgtattta aacaaaagtt
     7201 tagtgataaa gaaataaagg tattcaaaat ttttattaat agtaagaaag atttggaaaa
     7261 gatttgcaaa agggagggta tcgatttggt tatacaatgt ggtgactccc cttcacctcg
     7321 agaattagga aaaattgtga atacagtatg tgatactctc cagatacctt atatagttaa
     7381 cactggctac atgagtaatg tagttccttt gccagaattt tattatccaa ataatagata
     7441 caattttaat tataagcatc aattttcaga tgaaaagtta ctgttctcac aagttttgtc
     7501 taaagctgat tatatggtgg caattcaatc atcatttgta atgttaatgc aaataaaaaa
     7561 ttttatattt aatgaagaac ctatttatta tggttatagg ggatactata attctgtaac
     7621 attagcatgg gaggtggaaa aaattgacta aatatttaga attggattat tctcattcat
     7681 atattttgga aggttttaga aaaaatgatg cgtttattga aaatcacaaa gaatgccttg
     7741 atatgttatc agataagatt tggcgagata ataaatatat gaatacagaa aaaaacatat
     7801 catcaccgac acgaacaatc ttatcacgct atacttgtaa tattttatcg tctttgttta
     7861 ttgatattag caaaaatagc atagaaaaat tgatagtgac atgtgaaaat agcgatctta
     7921 attggattta taattattat aaatcggttt acaatggagt attttgtgaa ttgcgacaac
     7981 ttatacaaca gtactgtaag gtgaaggata agtatgaaaa gtggcttttt ataaaagcta
     8041 ttataaatca aggtattcgt caaaatgaga aagaagttgt tgaattcttt ttaaaacaat
     8101 tgaaggataa taatcaaggt atttttgatt acatcaatag ttttagtttt tatctattaa
     8161 agttttattc aaaagacaaa tctcgcattt ttttagaaga gcagctagat atagatgatt
     8221 ttttgggatc agataaagaa gattatgcta ggtctttagt ttttaagaat tatgcttctc
     8281 tattgcctga tacttctgaa ttaaaaagaa atattatgga aaaatgttta agtcaaacac
     8341 ctcaagatac agatttatgg aaagaatggt ttgaaactta tgccagtcag aaagagatta
     8401 gacaaaaagc aacggaaata tttaagcatg gttattcaga tatttcgtta ctaaaattag
     8461 ttaaaattga gcctgatgac actgagagtt taatcagaat gattatctta tgtaccagtg
     8521 acctaaatag taatattgca aggtatttga tttcattttt gagcgatggg cgattaaaag
     8581 aatttttgga gttatttgtt gaaaattttg actttttgaa tattattgag ggaaaaacta
     8641 gtgagattaa tttttagaat aattagaaaa gatattatat tctatattaa catttttgct
     8701 atcaatagcg atggtaagcg aagcattttt gatgcaattt attgttgatt caattaaatt
     8761 tggtgaaaag aagtatgttt tgatttctat ttttattgta atttttttga ttcttcagac
     8821 tttattgtat tactatcaac aatttcttac agctgtatta agtaaaaaat ctgcatatga
     8881 gtaccgtaaa ttaattttta aaaatattca gcagacttct cttaggcttg ttacaggtga
     8941 aaaaaatgat agtattctag cttctttgac aacgcaaatc gatcaaatag aatataatta
     9001 tttttattct gtttattggg gtggatattt gctttgccaa ttagtggtag ctatagttat
     9061 gtcgctttat ctaaatccat tgatgtctgt attaaccatt tgcttaagtc tccctaattt
     9121 atttgtagca cttcttttta aaaactcctt ggaacaaaag caagaagagc taattaatga
     9181 gacaaattcc tcaatagcag ttactcaaga tttaattgag ggaattactg attggaaaat
     9241 tgcaaataga gaacctaata tatttagagt gtttgaaagc aaaacattga agcttttaga
     9301 gaagcaagtg aaggtagaaa aatttcaata cattgttgtt agtttaaatc aattgttttc
     9361 aaatgtttta tattttggta gttggatagt aagtggattc ttgattatac gggggcaact
     9421 ttcattaggg agtttgatag cgttctctca attattagca agaatttctt accctgttta
     9481 cgcttcttcg gatttattgt caaaatatat tagcggtaaa aaaattgtag aaattctttc
     9541 taaagaattt atattttcag acgaaagcag aaattatcca gatgagttta atgttatttc
     9601 atttgaacat tattttacaa acattgacga aaaggatatg ccttttgata aagaatttta
     9661 caaaaataaa aaatatttgt tgaaagggaa aagtggtagt gggaaaacaa caattttaaa
     9721 atcgatttta cgagagtatg atgattatag tggaattgta aaaattaatg gggtagatgt
     9781 tagggatata aaagagagtg ttctttttga caatatagct tatgtttccc aaaaacctca
     9841 cttgttcagc gcaagtctta gagacaacct gacattattt tctgaagact tcactgataa
     9901 tgagatagtt gaggttttaa aatttgttga attatctaaa tgggctaata aagattgctt
     9961 ggatatgatg ctttcaaatg atgatataaa attatcagga ggggaggtca aaagggtttc
    10021 tttagctcga gccttattga tgaataagaa tattctgtta ttggatgagt tctctgctgg
    10081 gattgattat gaaacattgt taaaaataga gaagaaactc attgatttgg ataagatgat
    10141 tatttacgtg acgcatgtgg atatagatag atccggcaaa cagtttgatg atgtcattga
    10201 tttggattgt ctttttatat cctcgtgaca tgagagttga gtcagttgaa ttattttgtc
    10261 actctgttta gttcttgttg gtagtgtcag gtaatctttg ttaatttttc aggaagtcta
    10321 ttgaaatatc ctcttggtta tatattacga ggagtaatta atggatattc tcgagaattc
    10381 aatacaacaa tttgattcta aagttaagta tgtttcttta tttgcggaat caatctttta
    10441 tcagatacca tgagctattt tgcaggtgct atgatttctc ctttgtatgt gaggattatc
    10501 ggagatgata tgacagatgc tatgggggtt agacaggcaa ctgctggtgt agttaatatt
    10561 ctcagcaacc ttataggggg agttttgatt gggggtcatc agtcttcaga tgtttgtagg
    10621 tttgaatgct ttgacctttc tttttgcact gttgggtgtg gtgtttattc gtacttcttt
    10681 gagagatatt gaggaacaga tggaggttag taaaagatta gccgcaaaat cttttggaga
    10741 gcatttcctc cattcaatga agatattgtc aagttttcca gccatcatca aattaatatt
    10801 cattgcagct atgaaccaag tgattttaag cattattatc cctttaacga ctttgatgtt
    10861 aatccatcat ccatttatct tctttgacac tgggaaatcc attgcagcat tgtcagtggt
    10921 tattttttca ggaacgattg tgggaagttt tttgagtgga ggtatcctaa aaaatattcc
    10981 cataaaaatg attgtctact ttgaagaaat tatgcatctc tgtattctct tttcttggtt
    11041 gaccaatcat ttcctccttt tattggtagt tgtcttctta tgcgcagttg cagtaggaat
    11101 gatggggcct cggattttca accttgtatt ctcgatggtt ccagaagaag tcatgggaac
    11161 catccagtca gtgcttggag tattcaatgt tgttgtacca ggaattctta gcatgctcat
    11221 cgttgcctta gcgagtgcta caaacctaaa aatcacagtg cttccacttt ttatttttgt
    11281 attgactgcc ttagcaattg tgaaagcaat gaaaatagag taatagtagt atgaactact
    11341 gtctcttttt gggtgggagt tcattaccca tcagacgtcc ttgccagtct ctgtgtgggc
    11401 ttgggagttt tgtttatcga gtttcccttc tatgacaagc tccgcttcca atggcgattt
    11461 aaaggcaaac agaagtgagt atcgaattcc cttgaggaga aagaaatgaa agtcaatata
    11521 gcagatcttc atccgactca actatattta tcagaaaaga agttgcaaga tattcagatg
    11581 ctttaccagt cggcagaaac aatccaagtc gatccaatca gtattcttgc ctttggagat
    11641 tgtttgctga ttacagatgg gcatcacagg gcttatcagg ctttattggc aggtcgggat
    11701 acgatttctg ctgagtggga tagagatggt ggtgatgaac tatatcatct ctatgcgcaa
    11761 gcttgcgagg aaagaaagat ttactctgtt tttgatttaa aaaatcatat cttacctctt
    11821 tggatcggca tggtgagctt tttttgattg aagtcaagga gaagggagaa tggtttccaa
    11881 ttggggatat tacactatct caggataatc tccccattgt gattgggaat tccgcttacc
    11941 aacatcgagg acttggaaaa aagattctaa gtgctttgat tgaattggct cgagtaaaag
    12001 gatggaaaga attgagagtc aaggaaatct acacctacaa tcatgcttct aggaggtgtt
    12061 tcaagtcgct tggatttgtg gaaaatggag caacagaaaa aggaaggagt tttatattgg
    12121 aattagtcta atcctgccac tttctccccc ttaacctttg actattccgc aaaattatcg
    12181 taaaataaag agtaaatgat aaaatgaggt cagagtctgt tcgctctgac gatagtagta
    12241 taaatgagga gaaacgcttt ggaattagaa gtatttgctg ggcaagaaaa aagtgaacta
    12301 tctatgattg aggtagcgcg tgctatatta gaacttcgtg gtcgcgatca tgagatgcat
    12361 tttagcgatc ttgtaaacga aattcaaaac taccttggaa catcaaacag cgatatccgc
    12421 gaagctttgc ctctgttcta cacagagttg aactttgacg gtagcttcat ctcacttggg
    12481 gacaacaaat ggggtcttcg ttcatggtat ggtgtggacg aaatcgacga agaaatcatc
    12541 gctcttgaag aaaatgacga cgatgaagta gcaccaaaag ctaagaaaaa acgtgtcaat
    12601 gcctttatgg atggtgattc agatgccatt gactacaatg cagatgatcc agaagacgaa
    12661 gatgcatacg aagcagatcc agctctttca tacgatgatg aaaatccaga tgatgaaaaa
    12721 aatgaagtgg aagcttatga tgcagaaatc aacgaaatcg ctccagatga cttgggagaa
    12781 gatgtggatc tcaacgaaga cgacgacgag ttttcagatg acgacgctga aaccagcgag
    12841 gaataagagt tagctattga caattatcct atttttaggt atcatattgt ttgggcacct
    12901 cttttagagg tcggggctcc ctagttctta gggagctatt tttgtttttt caagaagtta
    12961 tcttcttgta ttttatactc aatgaaaatc aaagagcaag ctaggaaact ggccgtaggc
    13021 tgctcaaaac actgttttga ggttgtagat aagactgaca aagtcaggaa cacatatcta
    13081 cggcaaggcg acgttgacgc ggtttgaaga gattttcgaa gagtattagt tgtgaatctg
    13141 gtgcagtcgt cccagattat tcttattagt agggtcttgt tttctatatc ccctcgtagt
    13201 taacaagacc ttgagcattt tagaaagagg aatctatgtc tacgaaatat atttttgtaa
    13261 ctggtggtgt ggtatcgtct attgggaaag ggattgtggc agcgagtcta ggccgtctct
    13321 tgaaaaatcg tggtctcaaa gtaaccattc aaaagtttga cccttatatc aatattgatc
    13381 cgggaaccat gagtccttac cagcacgggg aagtttttgt gacagatgac ggagctgaga
    13441 cagatttgga cttgggtcac tatgaacgtt tcatcgatat caatctcaac aaatattcca
    13501 acgtgacaac tgggaaaatt tacagtgaag ttcttcgtaa agaacgccgt ggagaatacc
    13561 ttggggcaac tgttcaagtc attcctcata tcacagatgc tttgaaagaa aaaatcaagc
    13621 gtgccgctct aacgaccgac tctgatgtca ttatcacaga ggttggtgga acagtaggag
    13681 atatcgagtc cttgccattc ctagaggctc ttcgtcagat gaaggcagat gtgggtgcgg
    13741 ataatgtcat gtatatccat acaaccttgc ttccttacct caaggctgct ggtgaaatga
    13801 aaaccaaacc aacccaacac tctgtcaaag aattgcgtgg cttgggaatc caaccaaata
    13861 tgttggttat tcgtacagaa gagccagctg gtcaaggaat taaaaataaa ctggcccagt
    13921 tctgtgatgt ggcaccagaa gccgttatcg aatcgttgga tgttgaacac ctttaccaaa
    13981 ttccactgaa cttgcaggca caagggatgg accaaattgt ttgtgatcat ttgaaattag
    14041 acgcaccagc agcggatatg acagaatggt cagccatggt ggacaaggtc atgaacctca
    14101 agaaacaagt taagatttcc cttgttggta agtatgtgga gttgcaagat gcctatatct
    14161 cagtggtcga agccttgaaa cactctggct atgtcaatga tgtagaagtt aaaatcaatt
    14221 gggtcaatgc caatgatgtg acagcagaga atgtagcaga actcttgtct gatgcggacg
    14281 ggatcatcgt accaggtggt tttggtcaac gtggtacaga agggaaaatc caagccatcc
    14341 gctatgcgcg tgaaaatgat gttccaatgt tgggagtctg cttgggaatg cagttgacat
    14401 gtatcgagtt tgctcgtcac gttttaggtc ttgaaggtgc caattctgca gagcttgcac
    14461 cagaaacaaa ataccctatc attgatatca tgcgtgatca gattgatatt gaggatatgg
    14521 gtggaaccct tcgtttggga ctttatccgt ctaagttgaa acgtggctct aaggctgctg
    14581 ctgcttatca caatcaagaa gtggtgcaac gccgtcaccg tcaccgttat gagtttaata
    14641 atgccttccg tgagcagttt gaggcagcag gttttgtctt ttcaggagtt tctccagaca
    14701 atcgtttggt agaaatcgtg gaaattcctg aaaataaatt ctttgtagct tgtcagtatc
    14761 accctgaact gtcaagccgt ccaaaccgac cagaagaact ctacactgcc tttgttactg
    14821 cagcggttga gaacagcaat tagcaaaatc agaacctttg agaaaaatct cagaggtttt
    14881 ttgcatacga tgatattgca gtatatctga ggtagggggc ctctgtatgt acctgctacc
    14941 gttgaaatca atagcgactc cctcttgccc tgtgctagtg aatggattta tcagtatatt
    15001 gaaatgaaat aaaatttgaa caaattaatt cggaaagcca aatcaatttc tagcaaagtt
    15061 ttaggaactg gattgtatag tgaattgaaa taagatgtga acatctctat cagaaaagtc
    15121 aaattaattt atagaaatat tttagcagtc aagatgtact gttatagatt caatacatta
    15181 tactttttta atttaatcca
//
LOCUS       DAVBCW010000042        15154 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVBCW010000042 DAVBCW010000000
VERSION     DAVBCW010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15154
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.042"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(62..859)
                     /gene="proC"
                     /locus_tag="VQ514_001119"
     CDS             complement(62..859)
                     /gene="proC"
                     /locus_tag="VQ514_001119"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HET2499878.1"
                     /translation="MKIGFIGLGNMGASLTKSVLQARPSDEILLANRSQVKVDAFIAN
                     FGGQTSNNEEIFEEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSEIFCQLLAKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMEAVHQAYKRTQELGK"
     gene            complement(863..2125)
                     /locus_tag="VQ514_001120"
     CDS             complement(863..2125)
                     /locus_tag="VQ514_001120"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HET2499879.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAVSFLPRLEQ
                     VLVADRKEAGLEPIQFRLDSKASQFVSGQAAQAQDFDTEFLDYILAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVTGDGQIRE"
     gene            complement(2135..3265)
                     /locus_tag="VQ514_001121"
     CDS             complement(2135..3265)
                     /locus_tag="VQ514_001121"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HET2499880.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMVEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGVFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRFQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            complement(3348..5228)
                     /gene="cbpE"
                     /locus_tag="VQ514_001122"
                     /gene_synonym="pce"
     CDS             complement(3348..5228)
                     /gene="cbpE"
                     /locus_tag="VQ514_001122"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HET2499881.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRKDGFVNISTSYKPIPS
                     FQAGWHKSAYGNWWYQAPDSTGEYAVGWNEIEGEWYYFNQTGILLQNQWKKWNNHWFY
                     LTDSGASAKNWKKIDGIWYYFNKENQMEIGWVQDKEQWYYLDVDGSMKTGWLQYKGQW
                     YYFAPSGEMKTGWVKDKEAWYYMDSTGIMKTGEIEVAGHHYYLEESGAMKQGWLKKAN
                     DWYFYKTGGSRAIGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVPIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSE
                     SE"
     gene            complement(5231..6118)
                     /locus_tag="VQ514_001123"
     CDS             complement(5231..6118)
                     /locus_tag="VQ514_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET2499882.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLVLAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            complement(6108..6569)
                     /locus_tag="VQ514_001124"
     CDS             complement(6108..6569)
                     /locus_tag="VQ514_001124"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HET2499883.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMFLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            complement(6566..7474)
                     /locus_tag="VQ514_001125"
     CDS             complement(6566..7474)
                     /locus_tag="VQ514_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET2499884.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            7467..7664
                     /locus_tag="VQ514_001126"
     CDS             7467..7664
                     /locus_tag="VQ514_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2499885.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLNYLHYLPFSRQSY
                     FQNQFKKISGYTPLQYRKEKR"
     gene            complement(7716..8387)
                     /locus_tag="VQ514_001127"
     CDS             complement(7716..8387)
                     /locus_tag="VQ514_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2499886.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETVEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(8441..8662)
                     /locus_tag="VQ514_001128"
     CDS             complement(8441..8662)
                     /locus_tag="VQ514_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HET2499887.1"
                     /translation="MILMTKNINLTNEELELIQGGADSYGKNPNGRYDWEIEPVLTLP
                     VHGFCPRGTYDLGYIGGGNHLCKGSAARF"
     gene            8934..9743
                     /locus_tag="VQ514_001129"
     CDS             8934..9743
                     /locus_tag="VQ514_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2499888.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLGLDLQNEYVIVNNGCSTHQTSDWGLVDWQELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            complement(9924..10799)
                     /gene="aguB"
                     /locus_tag="VQ514_001130"
     CDS             complement(9924..10799)
                     /gene="aguB"
                     /locus_tag="VQ514_001130"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HET2499889.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKLLAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYNLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(10809..11894)
                     /gene="aguA"
                     /locus_tag="VQ514_001131"
     CDS             complement(10809..11894)
                     /gene="aguA"
                     /locus_tag="VQ514_001131"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HET2499890.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVV
                     AFVGPAEVVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEERYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            complement(11891..13018)
                     /gene="nspC"
                     /locus_tag="VQ514_001132"
     CDS             complement(11891..13018)
                     /gene="nspC"
                     /locus_tag="VQ514_001132"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HET2499891.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDEDMEELLGITDHIVF
                     NSERQLRKHGARCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFESQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            complement(13018..14277)
                     /locus_tag="VQ514_001133"
     CDS             complement(13018..14277)
                     /locus_tag="VQ514_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HET2499892.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVITLIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVEKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMLD"
     gene            complement(14274..15134)
                     /gene="speE"
                     /locus_tag="VQ514_001134"
     CDS             complement(14274..15134)
                     /gene="speE"
                     /locus_tag="VQ514_001134"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HET2499893.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGAFMLPKYVE
                     DILEEEEGKK"
BASE COUNT         4436 a         3505 c         2770 g         4443 t
ORIGIN      
        1 agtttgaatt tgattttcga agagtatcag ccaccataaa agaggcagat aaaactacct
       61 cttatttacc tagttcttgt gttcgtttat aggcttgatg aactgcctcc atgactgtgc
      121 ctcgaaaagc atgcgcttct aggcttgcta cgccagcaat agtcgaaccg cctggactac
      181 aaacttgatc tttcaaaact ccaggatgtt gctggctttc taggaccaat tgcccagctc
      241 ctaccacagt ttgggctgcc attttcaagg ctgtttctcg tggtaatccc gtctgaacac
      301 ctgcatctgc caaggcctca ataaagagat agacaaaggc tggtccacaa cctgcaagac
      361 ctgtcgctgc attgattaag ctttctccta attcaaccaa gagaccagcc ttggctaaaa
      421 gctgacaaaa gatctcactg tcctcagccc tgcaattagg agacaaggca taactaatca
      481 ctccttgccc gatagaagca ggggtattag gcatcatacg aataattcgg tgttggcttg
      541 ggataagact tgctagtttt tctaaggtca atccagctgc catcgaaatc aaaagaagac
      601 tttctctttt ttcaaggatg gtctggtatt gagaaagcag ttcagaaaac tgagcaggct
      661 taacacctag aaaaatcaca tctgcttctt cgaatatttc ttcattgtta gaagtctgac
      721 caccgaagtt agcgatgaaa gcatccacct tgacttgact acgattggca aggagaatct
      781 catctgacgg tctagcctgc aagacagact tggttaaact agcacccata ttccccaaac
      841 cgataaatcc aatcttcatc tcttactccc ttatctgtcc atcaccagtg accacatact
      901 tgtagctagt caattccttc aagcccatag gaccacgcgc gtgcaatttc tgagtagaaa
      961 tccccatttc acaaccaaga ccaaattgtc ctccatctgt gaaacgagtt gaggcattaa
     1021 catacaccgc tgcagagtcc acttgatctg taaagtatgc tgcagcttca gcattttccg
     1081 tcacaatggc atccgaatga tgggtactgt gggattcaat atgcgcaacc gcttcttcta
     1141 aactgctcac aaccttaaca gctagaatat agtctaaaaa ctcggtatca aagtcttgtg
     1201 cttgagcagc ttgacctgaa acaaactggc ttgctttgct atctaggcgg aattgaattg
     1261 gttccaaccc agcttctttt cgatctgcaa ccaacacttg ctccaagcga ggaaggaagc
     1321 ttactgcttt gttttcatga accagcagaa cctccatggc attacaaaca gaaggacgac
     1381 tggttttagc attgttgatg atagacagcg ccttgtcttc gtctgcatcc ttatccacat
     1441 agacatggac aatcccagtt cctgtctcga taacaggtac aatcgcattc tcaaccactg
     1501 cattgatcaa gccagctcct ccacgaggaa tgagaaggtc tagatagccc ttggccttca
     1561 tcatagcata actactttca cggctagtat cctccaccag ttgaatcaca tttggatgaa
     1621 tagtagtcgt ctccaagccc ttcttcaagg ctgtgacaat ggcatgggtt gtttgatagg
     1681 catccttacc actacgaaga acaaccgcat ttccactctt aagagtcaaa gcagccgcat
     1741 cagacgtcac atttggacgg ctttcataga taataccgat gacacccata gctacacgtt
     1801 tttttgtgat aaccaaacca ttttcaagct gacttgtttc taaaacttca ccgattggat
     1861 ctggtaaggc aaccacttca cgaattcctc ttgccatcgc ttctatacga tctgcatcca
     1921 aataaagacg atccaacatc acatctgaga ttttcccctt agccgctgcc atatcgaggg
     1981 cattagccgc taaaatttcc tcagtagcag ccactaagtg atcagccatg gctagcaagg
     2041 cttggttttt cacttcttca ctagctgtgt taatcgattt tttaacagcc tgtacctgtt
     2101 caaattgttc ttgtctactc accatagttt acctctaaaa ttctgtaaaa agtagttgga
     2161 tttcaggagt aatggaaatc cagtcgtcac ggtaaatcaa gacacccttg gctttttgaa
     2221 aacgcaacat atcctccaaa gcagatgctc caaattgcac gcgtcctttt ccaagtgatt
     2281 ttccactttc cttatcaaat actgtcacga tatcaccgta agaaaagact ccttctgctt
     2341 caacgatacc agataagaga agactctttc catgttgaga gagagcttcc gcagcccctt
     2401 tatcaaccca aatagaacct tgactctgag catagaaggc aagccattgt ttctgggtac
     2461 gaagcccctt ctcttgagca acaaagtaag aaccatcctc ggtctcctct gccgcctcaa
     2521 ccatggaatc tgatttcaag gatgagcaga tataaacagg aactcctgat tccgtcgcga
     2581 tagttgcagc cttgattttg gttaacatac ccccagttcc gtttgacgaa ccagctccac
     2641 cagccatatc aataatctca cgattgatgg tctcgattct ctccaagcgt ttggctcttg
     2701 gatctgaatt aggatttcca gtatagagac cgtccacatc tgtcaagaaa actaaaaggt
     2761 ctgcttggac catcgccgct acttgagcac ttagagtgtc attgtcccca accttgagct
     2821 catcaataac gacactatca ttctcattga tgataggaat tgccccacgg ttgagcaaaa
     2881 ccgacaaagc ctgatgggca tttttataac gacgcttatc cacaaagtca tcttgggtca
     2941 gcaagatttg tgcagaaacg atttgacgca agagaagatt ggtcgtatat tcttccaaca
     3001 aaagtccctg ccctaccgct gctgaagcct gtttatcagc aattttagtc ggacgctttt
     3061 taaatcctaa ggctccaaaa ccagccgcaa tggcacctga agacaccaaa atcaactcat
     3121 gaccagcctc gtgcagcata gccaactgct gggtaatatc ctttacctta ctacgtgata
     3181 aacttccatc ctcatttgtc agagaagaag tgcccacctt aaagacaatc cgtttgtatt
     3241 tcatagtctc actccgatta ttcatattta tccattataa catgaataac aggaaataga
     3301 aaagagttga taggaaaaag aaggccctaa aaccttcttt tctactacta ttctgattcc
     3361 gatttgttta cactcgtttc agagctttgt gaagttgagg atgtcaaatc ttgactagtt
     3421 gaagaatttg taacactttc atgttgactc gtttctttat tttcaagatc ctttttcact
     3481 acttctttgg attcttttat ttctgaatga cttgtagttt taattgtagc agatttctcg
     3541 atcggaacat ccactaacca ggcaccactt gaatccacag tatagccttc tggtgtctta
     3601 ccattcacaa gtaattgacc attttctttc aagaagtacc atttatcctt gtctttaatc
     3661 caacctatag ctcgtgaacc acctgtcttg tagaaatacc aatcatttgc ctttttaagc
     3721 cagccttgct tcatagctcc tgattcttct agatagtaat gatgtccagc aacttctatc
     3781 tcacctgtct tcatgatacc agtagaatcc atatagtacc aggcttcttt atcttttacc
     3841 cagcccgttt tcatttcccc tgatggagca aagtaatacc attgcccctt atattgaagc
     3901 caacctgtct tcatagagcc atcaacatcc aaataatacc actgctcttt atcttgaacc
     3961 caaccaattt ccatctgatt ttctttgtta aaataatacc agattccatc aattttcttc
     4021 caatttttag cagaagcacc agagtctgtc aaatagaacc aatgattgtt ccattttttc
     4081 cattgattct gtaacaagat acccgtttgg ttaaagtaat accattcacc ttcgatttca
     4141 ttccaaccga cagcatactc tcctgtagaa tcaggcgctt gataccacca gttcccatat
     4201 gcactcttat gccaaccagc ttgaaaactt ggaatcggct tgtaggatgt tgaaatattg
     4261 acaaaaccgt cttttcgaat atcaaaaact gttgcatcat agtctttgct ggctgcgttg
     4321 attctctcaa ttcctcgttc tttgagccaa ttaacatact cactatcaac accatttttc
     4381 caaggtagac tatccgaagt ttgaacaatc aaactcggac tcaaattttt aatgaaatcc
     4441 ttggtatttg atttgttggt atcatggtga tgattaaact tcatcaaatc aacttttcca
     4501 atgagaggac catacttgtc ttctgctcca tgaacattat ctaaatcgcc cccaaggtaa
     4561 attttcttgc cattgacttt caccacgcta atcaaggaat tggaattgtc atcccaaatt
     4621 ttctttaatt cacccgatga atcagtttca ttttcataat tatagagctg aatatccatg
     4681 tccccaaact gaaaatgagc atccccttgt gtgatatttt gaataactga aacacctttt
     4741 tctgcagcag tctgtaaaac cttatcatag ccatacagat tatcccatag acgttcagaa
     4801 ttagtaatac gactatcact atatttctta agatagactc ggtcaactgg ataggtagac
     4861 agtaattcat caacatttcc aatatgatca ctgtgggtat gggtcaccaa aataaaatca
     4921 agtttttgga cacccaattc cttcaaacga cgaaagacac ggtctgttag aacatgctta
     4981 taagacgttt caattccttc tctccatgga taacgagaat cacttccatc tgggaaatca
     5041 taatcttctc ctgtatccac catggcaaaa tgtccattgc tttcaagaat aatcgcatca
     5101 ctgccacctt cttgaacatt gataaagtgg attttatttc ctgaactttc ttgagcctga
     5161 acattcccat accatgacaa acctaaaaaa gcgcctacaa gtgctaaact agttaatttc
     5221 tttttcattc ttactttctc aatctctcca aggtttcctt aaaaatctct gggatatctg
     5281 ctttaaattc caaggtctta cctgttctcg gatgagtaaa acctaaagtc ttggcatgaa
     5341 gaaattgtcc atgtcctttc aaagtcttgc gaggaccata gacctcatca ccagcgactg
     5401 gatggccgat ataagccatg tggacacgga tttgatgagt gcgccctgtc tccagttgca
     5461 actctactaa gctataatcg ccaaagcgtt ccaagacgtg aaaacgcgtc actgcaggct
     5521 tccctttagc agttacagcc tgtttcttac ggtctttttc actccggcca atcggcgctt
     5581 caattacacc acgatcatta ggcagatttc catgaacaat cgcccaatat ttgcggagag
     5641 actttttatc tttgagttct tgggcaagta ctagatgcgc atcatcgttt ttagcaatca
     5701 tgagaagacc tgacgtatcc ttatcaatac ggtgaacaat ccctggacgc agaaccccat
     5761 tgatacccga caagtcctta atatgataca tgagggcatt tactagggtt ccactggtat
     5821 gaccagcact cgggtgcaca accattccct gaggtttgtt aacgacagcc acatcctcat
     5881 cttggtagac tatttctagc ggaagatcct cagccacata ctctaatacc tctggttctg
     5941 gcacatggta agtgacgaca tcaccctctt ggactgtgta tttagctttc ttgacttgac
     6001 cattgaccaa gacctggcct gatttaattt gttcattcgc gagactacgt gataattctg
     6061 acaaatctga caaagcctta tccaaacgca gaccaccagt ttcaatttta atttccattt
     6121 atttcctctt ttagcattgc aatcaataaa ataatcactc caaccgtcag atagctatct
     6181 gccacattga aaattgcaaa gttgataaag tcaaggtgga acatatccac aacaaagccc
     6241 tgactgaccc tgtcaataaa gtttccaaga ccacccgcga ttattagagt caaacccaag
     6301 aacatccaga atgagtcctc catgtgttta tgtaaatacc aaatggcacc tatcacgaca
     6361 accagagtaa tgacagcgaa taacagctgc tgatcttgta agatagaaaa ggctgcacct
     6421 cgattttgca ggtaggtcaa gctaacgaaa ttgggaatcc aggagcgcac ttcacccagt
     6481 ggaatctgct ggacgatata ggatttgacc aactgatcca gcccaattaa aagcagtaca
     6541 atgactgcca ctattgctct ttttttcatg atttcctctt ttgatcaaaa tattcttgca
     6601 tgacttctac gaagagagtc ccagcttgac taagctccac ttcttcacgt ttaacataga
     6661 ccatgcggtt atctaggtta tccttgagac gaataactgt aatgccatta acactgtcac
     6721 tatctaaaaa tccagaacct gtcgcatagg cgtccgtccg ctccaaaata ccattcaagg
     6781 tggcacggtc tgtcacatta aacatctgtg agctagcgct ggtatcgaca aagttctctg
     6841 aataataaag gtactcgtct ttctcttgag tgaaacgaac cgttggtaaa tccgctaaat
     6901 cctccatgac taattcctct ttctgggcta aaggatgacc ctcacagaga taaatatggg
     6961 tatggaaagg aatcaattcg atgacctcca gacctaactt ttcaacccgt tgcataatcc
     7021 cctttttatt ttgattgttg aggtagataa tcccaatctc actatgccct tgcgccactt
     7081 catctaatat ttgaacagta gttgattcaa aaatacggaa gttcttatag tcaggatagc
     7141 gctctgaaaa ggccgtaata gttggtggta agaagtcata gtgctggcta gcaacggaaa
     7201 attcatcttt ttcttcttca ggattggcat actgattttg aaaaatatca aatcctttaa
     7261 ccaattcttg cgctttttca taaaattcca tcccacgacg ggtcaagaaa gtccctgagc
     7321 tggtccgacg gaaaatctta aagcccaact ctttttccaa atcacgaaca gaaatagaca
     7381 gactcggctg actaacatac atcttttcag cagcttcacg aaaagtacca ctattggcaa
     7441 tagccacaac atagcgtaat tgttgaatgt tcatcttcta cccccaactt ctttatcttt
     7501 tcattatacc atattttaga agttttccaa aaaggaaaaa agaacatcct attcttctta
     7561 actatcttca ctatctgcct ttctcacgcc aatcttattt tcaaaatcaa ttcaaaaaaa
     7621 taagtggcta cacaccactt cagtatcgga aagaaaaacg ttgactattt gtgaaaaaag
     7681 aaaatgccgg aaaattccga cattttttta gtaagctaac ttcctgaaaa taggagtaac
     7741 caaacatccc agaaggcagt gatattgatg agaatatgaa ctagtattgg atagtagaga
     7801 tttttagtca tgcgatacaa tagagctaaa atcagacctc cactagcata aatgaaaaaa
     7861 gcaagaggag ttaaagcaaa attgatatga atataaccga aaataatagc agaaagcaaa
     7921 acatctccgt accaaggtga gtttttgaaa aaggttgtca taagcacacc tcgataaatc
     7981 aattcctcag caataggggc gatgaagcaa acgatgagca agaaataagg gaactcctgt
     8041 ctccccatca tttctaccgt ttcattcaaa gaaatctgat ttgaagacag ggatatgaaa
     8101 tacgaaaaga ggaagtcaga catatatgaa atgatgtagc ccagtaaaag gtagatgaag
     8161 tacctcagct gccacttgaa atgaaaaatt ttctttcctg caaaaaccag tagatagatg
     8221 accgctaaga aaagaactcc gctctccatc aaaagcagaa tctgaaaaaa ttcacgactt
     8281 gctggtagat agggctttgc gaggtgattg aaaactctcc aaaaccaagt tgatttgtaa
     8341 aaaattaagg acaatgctag taaaatttga atagcccgtt ttttcatatt aaattctctg
     8401 ctttctccac ttatttcttc atattactaa taaattttac ttaaaatctc gcagcacttc
     8461 ctttgcaaag atgattgcct cctccaatat atcctaaatc ataggtgcct ctgggacaaa
     8521 atccatgaac cggcagagtt aatactggtt ctatttccca atcgtaccta ccattaggat
     8581 ttttaccata tgaatctgct ccaccttgta tcagctctaa ttcttcattt gttagattta
     8641 tattttttgt cattaaaatc atgatatatt tcctccatta ttcttcagat tagttgagta
     8701 atctgatact catacctact tacaaaaaaa ctattatatt aagttggttt tttaatttgt
     8761 ctagttgcaa cattgacaaa catagtatag catatctttg cgaaatatcc tcttcaaatc
     8821 atgaattgtc atcaaaacat cttaaactat aaaatcaatt agtctcaagc tttctatcaa
     8881 tttcttctcc aaaatatgct ataataatag caaaagataa agaaggaaga cctatgatta
     8941 aactactagc cttggatatg gacggcaccc tcctcaatga agccaaggaa atcccacaag
     9001 ctcacattac tgctattcac aaagccattg aaaaaggtgt caaactggtt ctctgtacgg
     9061 gtcgccccct tttcggtgtc ctcccctact acaaaaaact gggactcgac ctccagaatg
     9121 agtatgtgat tgttaacaac ggttgttcaa ctcaccagac tagcgactgg ggcttggttg
     9181 actggcaaga acttagtcca gctgacatcg aatacctcta tgaccttgct gaaaagagtg
     9241 atgttcagtt gacacttttt gacgagtcac attattttgt tctcggtgga aagcccaatc
     9301 aagttattga aaatgatgct aaactagtat tttcagacct gactgaaatt tctcttgagg
     9361 aagcgactag tggaaagttc cggatgttcc aaggtatgtt tttaggaaca aaagaacaaa
     9421 cagacgattt tgagcagcgt tttgctgaag agctttgcca acgattcagt ggagttcgtt
     9481 cgcagcctgt catttatgaa gcaatgccac ttggaacgac aaaggctact gctctttcac
     9541 gactagctga gattttgaag attgattcct cagagattat ggccatgggc gatgctaata
     9601 acgatatcga aatgctccag tttgcagggc ttgggattgc aatgggaaat gccagcgatt
     9661 atgtcaaatc tcttgcggat gccgttacct caagcaacga agaagacggc gttgcgcgtg
     9721 ctattgagaa atatattcta taaaaaagaa aagcaaatag acagaagtta ctgctatttg
     9781 cttttttgct aatattttaa aatacggact aatcaataga gaagaatagc gaatcataaa
     9841 aatcgattta ctagatgcca tagtatctta tagttgctaa taagaagtta gtctagcaga
     9901 atgaatctct catttctccc acactaatct gtaatttgtc tatacatttc tggtcttcta
     9961 tctcgaaaca agccccagtt taggcgttca cttgctccct tgtctaggtt ataagtagct
    10021 aacagaacag cttcttcttg tctttcagct cgttctagaa tagctcctgt ttcatccgtc
    10081 ataaaggagg aaccgtagaa gtcaagactg gaactctgtc cgccattttc ctcactagga
    10141 gtaacctcct ctaaaccata acgattggct gcgatgactg gaacaatatt cgctgctgcg
    10201 tgcccttgca tagtacgttg ccagtgacca caactatctg tatccaaaat tggctctgaa
    10261 ccgatagctg taggataaaa gagcaattca gcaccattca atgcaagaca gcgcgctgtt
    10321 tcagggaacc attgatccca acagataccg ataccaatct tagcatagcg agtattccag
    10381 accttgaaac cagtgttacc aggcgtgaaa tagaattttt cttgataata atggtcatct
    10441 ggtatatggg tctttcgata aacgcccagc acttccccat ctgcatcaat gacggcaata
    10501 gagttataca agacattacc atctttttca tagaaactga ttggtaaaac aacttgtagt
    10561 tccttagcaa gcaacttaaa atgctgaatg gcagtatttt ccgctacaga ttgggcatac
    10621 tggtagtagt catactgacg ttcctgacag aaatagggat gttcaaacaa ctcgggcaag
    10681 agaataattt gggctccttg ctcagcagcc tgacgtacta aacgctctgc ggtttggata
    10741 tttgttgcca catccttagc gcattgcatc tgaatggttg caactcttac atttctcatc
    10801 tttttctcct attctggaat ttgttgggtg atacagtgga tattgccacc acctaagaga
    10861 atatctctgg ctggtattcc gacaacttta cggtctggga aacacttgct gaggatatct
    10921 aaggccactt ggtcgtttac atcctcaaac tgtggaacca agacagcctt gttggcgata
    10981 taaaagttta cgtaggaagc tgctagtcgt tcacctgcgt atcgctcttc ttctccttct
    11041 tcatagatgt agcctggcaa atcttcttct gtcacaactt gtcgaactgc agggataggc
    11101 aatttatgaa tggtgaagtg acaacctttt gcatctgttt cctgttctaa gagttcgaga
    11161 tctgcttttg acatggcata ctggggatca ttttcgtcat ctgtccaagc caaaacaacc
    11221 tcagcaggac caacaaaggc aacaacatta tcgacgtgtt cattggtttc atcctgataa
    11281 ataccataag gaagccaaat aactttttca gcaccaagac tttctaataa tgtgttttca
    11341 atctcctctt tagtcaagtt aggattgcga ccaggactaa gcaagcaact ttcagttacg
    11401 agaatagttc cttgaccatc gctatggatt gcgcctcctt ccagtacaaa aggtttagca
    11461 tcatagacag gcctttccaa ggcctcagca aaacgactgg ctacttggtc atcctcttca
    11521 taatcttgat aaagaccatc ataggtgcct ccccaagcat taaaggccca atccacggct
    11581 aatttcttac ctttatcatt gacgagaatg gttgggccag tatcacgcgc ccaggcatca
    11641 ttggtgggaa tgtctaaata aacaaccttg tctccaagat aggattgggc ttcagataga
    11701 taggcctgct ccaccaaaag atagactctt tccccttctg ctatggtctc gatgatctga
    11761 gtaaatgctc ttttagcagc ctttccttga aaaggccatg atcctggtcg agtcggccat
    11821 atcatgaggg taccatgatg gggttcgtac tctgctggca tgtgatagcc taattttttt
    11881 ggactgtcca tcatgataat ctccctttaa agtcttgata gccaaaagct ttgagtaagc
    11941 tacagtctcc ctgttcgtcc atgagataga gacttggcaa tccaatacca ttaaaggtat
    12001 tatttttgac aaaagaataa atggccatgt cttgaaaata aagtctgtct ccgatttgga
    12061 ctggattttc aaaactataa tcaccaatca catcgccagt cagacaggta ttagaagaaa
    12121 gtctgtaggt atgggctttt tcctgtgact caaagccatt tctcaaaggt ggacgatagg
    12181 gcatctcaag tacatcaggc atatggcagg tcgcagaggc gtctaaaacc aagatttcca
    12241 taccgttttc tacaatatct aatacctcag ttgctaaata acccgcatta agcgcaatgg
    12301 cttcaccagg ctcgatatag atttcaagat tgtaagtttt tcggatacgc ttgatttctg
    12361 aaatcagcaa atccacatcg taaccttctc ttgtaatatg atgaccacca cccatattga
    12421 gccattttac ctcatgtaag tagggaccaa actgttcttc tactgctttc aaagttgttt
    12481 gtaaatcatc tgctccctgc tcgcaaaggg tatgaaaatg aagtccgtca accaaatcta
    12541 gcaaatcgct cggaatcttg tctatagtaa ctccaaagcg agaaccaggt gcacaagggt
    12601 catagagcgc gtgatctcct tgagttgaac actgagggtt gaggcgcaaa ccaacactga
    12661 caccagcctc tcgacaacga gccccatgct tacgcaactg tctctctgag ttaaagacga
    12721 tatggtctgt tatcccaagc aattcctcca tgtcctcatc cttgaaagct ggtgcaaaaa
    12781 catggacttc accaggaaat tcttcccttg ccaatttggc ctcatagagt ccactagctg
    12841 tcgtacctga tagatactgg ctaatcaagg gataagtttt gtagagggaa tatgccttct
    12901 gggcaagcaa gaccttgcaa ccggcctctt cttgtacata ttgtagaatg cggcaattag
    12961 cttctaactt ggccaagtca ataacatagg ctggtgttgg tacttgttct aacttcatta
    13021 gtccaacatt tgtggatttt caaccacaac ccatggcaaa ccatactcat tcaaagcttc
    13081 catgaatgga tctggatcta actcctcaag gttatacact ccagcttgtt tccaagttcc
    13141 gttcatgact aattttgtcc caatcatggc tggaacgcct gtcgtgtatg aaattgcttg
    13201 tgacccgact tctgcatagc actcttgatg gtcgcaaaca ttgtagatgt agatagtctt
    13261 ttcaacgcca tctttgacac ctgtaaagat acatccaata ttggttttac ctacagtacg
    13321 tgggccaagg ctagcaggat ctggaagcaa ggctttcaaa aattgaattg gaacaatttc
    13381 ttggccgtta aagttaatgg tatccgtacg aaggagtcca acattttcaa gacatttcat
    13441 gtgcgtcaag taagattgac caaaagtcat aaagaaacga atgcgtttga cacctggaat
    13501 gttcttggcc aatgattcga tttcttcatg gtgaaggaga tacatatctt tttgtccaac
    13561 ttgagggaaa tcatactcac gcttgataga catagcttcg acttcgaccc atttcccatc
    13621 ttcccagtaa gaacctggcg cagaaacctc acggagatta atttctgggt tgaagttggt
    13681 tgcaaatgga taaccgtggt caccgccatt acagtctaaa atgtcgatat aatggatttc
    13741 atcaaaatag tgtttgaggg cataagctga aaagacactg gttacacctg ggtcaaaacc
    13801 agaaccaaga agagcagtca agcctgcttc tttgaatttc tcttgataag cccactgcca
    13861 tgagtagtca aagtaggctg taaaaccaag ttccttacaa cgtttttcgt agatagcacg
    13921 ccactcaggg tcttctgtgt cttctgcttc gtagttggct gtatcgatat agtgaacacc
    13981 tgttgccaaa caagcatcca taatggttaa atcttgataa ggcagagcta cattcaaaac
    14041 agcttctggt ttgtagcttt caatcaaggt aatgacctct tctaccttgt cagcatcaag
    14101 tgctgcagtt tcaattttag tacttgtttt gccttctagc ttcgctttca agtcatcgca
    14161 ttttgacttg gtacggctag caatcataat ctctgtaaac gtttcgctat cttgacaaat
    14221 ctttgaaata gcaacttggg caacgccccc acaaccaata actagtaaac gactcatttt
    14281 tttccttcct cttcttctaa aatgtcctca acatacttgg gcaacataaa ggctcccacg
    14341 tgtaagtttg cagtgtagta ttctgtgaaa agctggcgtt ttttccagcc ttccttgtca
    14401 aaatctttga cagggtggta ttttttcgat gcaaatccaa acaaccaata gccagctggg
    14461 ctagttggaa tatgggcctg ataaacccga ctgattggaa aggcttgatt gaccttgcgg
    14521 tgcatgcttc ggcaggccga ctcatcctcg tcaaagaagg gactcccatg ctggtaaatc
    14581 atgatgccgt cttccttcag agctcgataa ctattgccgt agaattcctt ggtaaagagt
    14641 ccttccgtat ggccaaatgg atctgtcgca tcgttgatga taatatcgta atcatcttcg
    14701 cagtttcgca aaaagcgtag cccattttgg tagtaaatgg taacacgagg atcatctagc
    14761 cctgcagcaa agtctgggaa atactcacga cagacctcga ccaacatctc atccggttcc
    14821 acgatatcga tttgttccag ttcaggatag agtgttaata cttgggcaac accgccgtca
    14881 ccacccccaa taaccaatac tttctttgga tttgggtgga cagccatggg aacgtgaacg
    14941 gtcatttcat tgtagacgaa atcatccgca tctgagaaca agacatggcc atttaaaatc
    15001 agtattttcc caaaagctgg cgtatccaag acttcgatat cctgccattc actttttcca
    15061 gcgtaaagtt gcttggctgt tctcagagac aatttgacat ctggagtatg aacttcagaa
    15121 aaccataaat ccatctattt ctccttatat tata
//
LOCUS       DAVBCW010000043        15035 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVBCW010000043 DAVBCW010000000
VERSION     DAVBCW010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15035
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.043"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(20..568)
                     /locus_tag="VQ514_001135"
     CDS             complement(20..568)
                     /locus_tag="VQ514_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HET2499894.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLVGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            complement(583..1431)
                     /locus_tag="VQ514_001136"
     CDS             complement(583..1431)
                     /locus_tag="VQ514_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HET2499895.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            1619..2968
                     /gene="trkA"
                     /locus_tag="VQ514_001137"
     CDS             1619..2968
                     /gene="trkA"
                     /locus_tag="VQ514_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HET2499896.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            2972..4411
                     /locus_tag="VQ514_001138"
     CDS             2972..4411
                     /locus_tag="VQ514_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HET2499897.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDVSVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRIKAFFGDEELRAYL
                     VIVVVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKTVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(4537..6216)
                     /locus_tag="VQ514_001139"
     CDS             complement(4537..6216)
                     /locus_tag="VQ514_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET2499898.1"
                     /translation="MDKLVAAIEKQQGKFEKISTNNYMMAIKDGFIATMPLIMFSSFL
                     MIIIMIPKNFGVELPSPAIAWMRKVYMLTMGVLGVIVSGTVAKSLAGNVNRKMPPGKV
                     INDTSAMLAAICGYLVLTVTLVVDEKTGSTSLSTNYLGSQGLITSFVSTFITVNVYRF
                     CIKRDITIHLPKEVPGAISQAFRDIFPFSFVLLISGLLDIVSRFSLDVPFAQVFQQLL
                     TPIFKGAESYPAMMLIWFMCALLWFVGIHGPSIVLPAVTALQLSNMEENAQLLANGQF
                     PYHSLTPNFGNYIAAIGGTGATFVVPFILIFFMRSKQLKSVGKATITPVLFAVNEPLL
                     FGMPVILNPYLFVPFLMTPPVNVFLGKVFIDFFGMNGFYIQLPWAFPGPLGLLIGTNF
                     QSISFVFLSLILVVDILIYLPFCRAYDRQLLMKEDVASSNDIILEEETSEIMPGELDE
                     IKSKELKVLVLCAGSGTSAQLANAINEGAQLAEVRVIANSGAYGAHYDIMGVYDLIIL
                     APQVRSYYREMKVDAERLGIQIVATRGMEYIHLTKSPSKSLQFVLEHYQAV"
     gene            complement(6286..7698)
                     /gene="lacG"
                     /locus_tag="VQ514_001140"
     CDS             complement(6286..7698)
                     /gene="lacG"
                     /locus_tag="VQ514_001140"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HET2499899.1"
                     /translation="MENLQVKALPKEFLLGAATAAYQVEGATRVDGKGINMWDVYLQE
                     NSPFLPDPASDFYYRYEEDIALAAEHGLQALRLSISWVRIFPDIDGEPNILAVRYYHR
                     VFQSCLKYNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
                     TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGKVGC
                     IHALKPGYPIDGQKENILAAKRYDVYNNKFLLDGTFLGYYSDDTLFHLNQILEANNSS
                     FIIEDGDLEIMKRAAPLNTMFGMNYYRSEFIREYKGENRQEFNSTGIKGQSSFKLNAL
                     GEFVKKPGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGEN
                     DIIDDSKRIRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYGLFFVDY
                     ETQKRYIKKSALWVKGLKRN"
     gene            complement(7708..8052)
                     /locus_tag="VQ514_001141"
     CDS             complement(7708..8052)
                     /locus_tag="VQ514_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2499900.1"
                     /translation="MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELV
                     ESANDSIVSAHREQTNLLAEEASGDNFEVSFIMIHGQDTLMTTMLLYDQVKFFIDEYE
                     RIRKIEEHIGLQ"
     gene            complement(8220..10106)
                     /locus_tag="VQ514_001142"
     CDS             complement(8220..10106)
                     /locus_tag="VQ514_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HET2499901.1"
                     /translation="MVIRVFDQQKNTYSSFALEELSYYMNRVFKTNIELVEEKEADIF
                     VGLVNKEDRKDHVLISLDKGKGRIESNTIVGLLIGIYRMFHEFGVVYTRPGRRHDFVP
                     ELRFEDFLDKQLSIDETASYYHRGVCIEGADSFENILDFIDWLPKIGMNSFFIQFENP
                     YSFLKRWYEHEFNPYLNKEKFSNELVQELSDRLDKELQKRGLIHHRVGHGWTGEVLGY
                     SSKFGWESGLSISEEKKPYVAEINGKRELFNTAPILTSLDFSNPDVADKMVEIIKDYA
                     KKRPDVNYLHVWLSDARNNICECENCRQELVSDQYIRILNQLDRALTSEGLDTKICFL
                     LYHELLWAPQKEKLDNPERFTMMFAPITRTFEMSYADVDFDNSIPTPKPYMRNKIILP
                     NSLEENLSYLFEWQKTFKGDSFVYDYPLGRAHYGDLGYMKISQTIYRDVSYLSNLHLN
                     GYISCQELRAGFPHNFPNYVMGQMLWKKTRSYEELIEEYFFALYGENWQSVVEYLEKL
                     SIYSSCDYFNAIGSRQNDVLANHYYIAYNLADNFLPIIEENISKLLNSQKDEWKQLSY
                     HREYVVKMAKALYLQATGKTRQAQDEWRNVLNYIRGHELLFQSNLDVYRVIEVAKNYA
                     GFHL"
     gene            complement(10108..11430)
                     /locus_tag="VQ514_001143"
     CDS             complement(10108..11430)
                     /locus_tag="VQ514_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2499902.1"
                     /translation="MNNILAFLETKVAPFGEKVGNQRHLKAIREGFMMAMPLILVGSL
                     FLILISWPQEAFTNWLNSVGLLSILTTMNQSTVAIISLVACFGIAYRLSEGYGTDGPS
                     AGIIALSSFVLMAPRFSSMVYDKNGEQVKQLFGGAIPFSSLNASSLFMAITIGLVTAE
                     IYRMFIQRGITIKMPSGVPDVVSKSFSALLPGFTTFVLWALVLKGLEAAGVAGGLNGL
                     LGAIVGTPLKLIAGTLPGMILCVIVNSFFWFCGVNGGQVLNAFVDPVWLQFTTENQEA
                     VAAGQTLQHIITLPFKDLFVFIGGGGATIGLAICLFLFSKSRANKTLGKLAIIPSIFN
                     INTAILFTFPTVLNPIMLIPFIATPTINALITYVSMAVGLVPYTTGVILPWTMPPIIG
                     GFLATGASWRGALLQVVLILVSVAIYYPFFKIADKRNLEKEKATVGGK"
     gene            11598..12821
                     /locus_tag="VQ514_001144"
     CDS             11598..12821
                     /locus_tag="VQ514_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HET2499903.1"
                     /translation="MITKQSAFLQNRATILEFLYRNPATSRTDIVNETGLTPATTTNI
                     IKELSEQSLIYETGDEFSEFSGSGRRRKTISITDNIPYVVGGIEINVLGIFLSLCDLQ
                     GKTLFETEILNEDYPISEINSTITNMIKTAIEYVPLETKLLGFGLSIPGHYNKDSGSI
                     ITNNPIWESFNLLNVIKRFNFPFIVKNNIDCMAIGQYLFNPHNTPDNFIFLHAGLGIY
                     TSFFTKEKIGASKNPYIGEIGHTIVELNGQYCECGKKGCLQTYISDAWLIKHAQLLFK
                     NSQLTVLKSLVKTEKDIHLDTLLTAYNLGDSALRQQIDKGVNLLATSIANLLLINPAD
                     KIYINSQLLNYQPFTHEVRDKIQDQLHFVPFTRNIEIEILPYNKHRGSIGACALAIVA
                     FFIEHSNVLQDIISP"
     gene            13120..13290
                     /locus_tag="VQ514_001145"
     CDS             13120..13290
                     /locus_tag="VQ514_001145"
                     /inference="COORDINATES: protein motif:HMM:NF024172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2499904.1"
                     /translation="MTKQIPKFTKDTAQLYTCKWLLYNKVTKMYDHTIVNHSVREYIT
                     DSISTNTIEEAE"
     gene            13902..14186
                     /locus_tag="VQ514_001146"
     CDS             13902..14186
                     /locus_tag="VQ514_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499905.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            14179..14417
                     /locus_tag="VQ514_001147"
                     /pseudo
     CDS             14179..14417
                     /locus_tag="VQ514_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397100.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14513..14731
                     /locus_tag="VQ514_001148"
     CDS             14513..14731
                     /locus_tag="VQ514_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499906.1"
                     /translation="MMSIREQDLKDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVL
                     LEGFSYAYGMDWLEKFFKENQDKLREFY"
BASE COUNT         4943 a         3104 c         2405 g         4583 t
ORIGIN      
        1 ctttgatttt cattgagtat tagtcctttt tcaaacttcc tgcacgagtt tgggttcctg
       61 cataggcaag taagagaaga gttcctgcaa tagctacaga tacaccattg gcaattcccg
      121 caacaattcc ttgtgcaaat actttttctg ccgcttcttg ataaatcaca acatctccaa
      181 gtggtgccaa gacaccccaa acaagggcat ttgcaagtag ttgaatgagg ttaaaaataa
      241 gaatatcttt ccagtcaaaa acaccattga tcacgcgaac gtactttcta aaaagtccca
      301 caactaaacc aaagagtccg ctagcgataa tccaagtcca ccatagacca taaccaacaa
      361 gagagtcctt gattgcatga ccaatcaacc caacaagcaa accgataatc ggtccaaaaa
      421 taatagaaag tagcgcttgt accgcatact gaagctggat gcttgtattt ggaacagggg
      481 ttggaatgtt gatcatcccg atgacgacaa agagggcagc gccaattccg acagcaacaa
      541 cttgtttaat tgtaaatttg atttccatac tattctccta ttttatcctt ctattttctt
      601 tatttcaatg gtccaagatg aaccgacacc tacattatag gccttggcaa aggaaccttg
      661 gttgatagcc aaacctaaac gatagagaga gttgatgtaa aggatgggtt gcccaattct
      721 cacatctgca aatgatttgc catagacaac ctgattttga tagaccagca tatcagcatg
      781 atagatggtc acttcaaaac gatcaccaaa ttctggttcc aacttgtaaa attcttcccg
      841 tgtgatagag gtccaaagcg aaccgaaacg cacatccaga atatcaatgg ctcccttcac
      901 cagatgatct tctatgatgg tcgctacgac tggaagctct acaatctgtt ccacactgag
      961 ctctggccct acttcctcaa aagtaatgtg accactggcc agtttagcac cagtataggc
     1021 atagacatca cgaccgtgga aggtataaga atgctctgtg ttttgacgcc tattggccac
     1081 ctcagaaatc tcacgaatgg ctacaatgcc aacgtgtttc ttgataaagg aaagcgtccc
     1141 attatctggc gtgacaatgt attgattttt tgcagtcttg gcaactacac tcttacgttt
     1201 cgaaccgaca cctggatcga caaccgatac aaacgtcgtt ccctcaggcc agtaatccac
     1261 cgtctgaaag agacgatagc tcccctcaaa aatattataa ggcgtgatat cgtgcgtcaa
     1321 gtgatggatt tttaaggttg gagactcttc taaagccact ccaatcatag ccgataccgc
     1381 accatcaacc agaccaaagt ctgattgtaa taccagtaaa ttattattca ttttatctcc
     1441 ttgtatatcc tttatcatac catatttcag agaaagtcgc taatagctat ttcaattggt
     1501 tagggtatag ttttatctta tagcgaattt tcactaaaag ctcttgttat tagctagtag
     1561 gtgcattttt tcttgaaaaa gggtatgata gttacatcat gaaaaagtag gttttattat
     1621 gaaaattatc cttgtcggag ggggaaaagt tggttttgcc ctctgtcgct ccttggttgc
     1681 agaaaagcat gatgttttgc tgattgagca agacgaagct gttctcaatc atattgtcag
     1741 tcgctttgat atcattggta tccttggtaa cggggccgat tttgccattc ttgagcaagc
     1801 cagcgtccaa gattgtgata tctttatcgc cctgactgag cacgatgaag tcaacatgat
     1861 tgcagcagtt ctagccaaga aaatgggagc taaagaaact atcgttcggg tgcggaaccc
     1921 tgaatattct aactcttatt tcaaggaaaa gaatattctc ggtttttctc ttatcgttaa
     1981 tcctgagctc ttggctgccc gtgctatcgc gaatatcatt gacttcccca acgccctgtc
     2041 tgtcgaacgc tttgctggtg gacgcgttag cctcatggaa tttgtcgtca agtccaccag
     2101 cggtctttgc caaatgccca tttctgattt tcgtaaaaaa tttggtaatg tcattgtctg
     2161 tgcgatagag agggatcatc aaattatcat tccaagcggt gacatgactg tacaggataa
     2221 agatagaatc tttgtcactg gtaaccgtgt cgatatgata ctcttccata attattttaa
     2281 atcacgcgcc gtgaagagcc ttctcatcgt tggggcaggt agaattgcct attatctact
     2341 tggtattctc aaagatagtc gtatcgatac aaaagtcatt gaaatcaatc ctgaaatcgc
     2401 cagcttcttt agcgagaaat tcccaaatct ctacatcgtt caaggagatg gaaccgcaaa
     2461 agatatcctg ctggaagaaa gtgctcaaca ctatgatgcc gttgcgactc taacaggagt
     2521 cgatgaggaa aatctgatta catctatgtt ccttgacagg gtaggtgtac agaaaaatat
     2581 tactaaggtc aatcgtacca gtctcctcga gattatcaat gcgcctgatt tttcaagtat
     2641 catcacacct aaaagcatcg ctgtagatac gattatgcac tttattcgtg gtcgagttaa
     2701 tgcccagtat tcagaccttc aagccatgca ccatctagcc aatggccaaa tcgaaaccct
     2761 gcaattccat atcaaggaag ccaataaaat gactgccaaa cctctttctc aactgaaatt
     2821 gaaaaaaggg gttcttattg cagccattat tcgaaagggc aagactattt tcccaactgg
     2881 ggaggatatg ttggaagttg gagacaagct cctagtaaca accttgttgc caaacatcac
     2941 caagatttat gacttgattg cgaggtaaga aatgaataaa agtatgattc gttacctcct
     3001 ttcaaagtta cttttgattg aagctgttct cctcttggtt cctgtggcta tcgctgtcta
     3061 ttaccgtgaa tcgagccaag tctttacagc cctcttttcg accataggga ttctcgtatt
     3121 gttaggcggt tcagggagtt tacagaagcc aaaaaatcaa cggatttatg ccaaggaggg
     3181 agtcttgatc gttgccctct gttggatcct ttggtctttc tttggtggtc tcccctttgt
     3241 cttttctggg caaatcccca gccttattga tgcctttttt gagattagtt ccggctttac
     3301 gactactgga gcaagtattt tgaacgacgt ttcagttctc agtcgttccc tcctcttctg
     3361 gcgaagtttt acccacttga ttggagggat gggagtgctt gtttttgcac ttgctattat
     3421 ggacaatgcc aaaaatagcc acctagaggt gatgaaggct gaggttccag gtcctgtttt
     3481 tggcaaggtt gtatccaaac taaaaaacac tgcccagatt ctctatctcc tttatctagc
     3541 tctcttctcc ctctttgtca tcatctatta tctagctggt atgcctctct acgatagttt
     3601 tgtcattgct atggggacag cgggaactgg aggctttacc gtctataacg acggaattgc
     3661 ccactatggc agctcactga ttacctatct ggtcagtatc ggagttctgg tttttggagt
     3721 aaatttcaac ctctactact acctcatgct ccgtcgcatc aaggccttct ttggagatga
     3781 agagcttagg gcttacttgg tcattgtagt ggtttctaca ggcttgatta gcctcaacac
     3841 cctctacctc tacccagggt tttcaaagag ctttgaaatg accttcttcc aggtttccaa
     3901 catcattaca acaactggtt ttggatatgg agatattacc aactggcccc tcttctccca
     3961 gtttatcctc cttttcctca tggcaatcgg tggctctgct ggatcaactg caggtggact
     4021 caagattatt cgaggcctca tcctttcaaa aattgccaaa aaccaaattt tgtcaatcct
     4081 atcgccccac cgtgttttga ccctccatgt taataaaacg gtgattgaca aagataccca
     4141 gcataagatt ctcaagtact ttgtcatcta tgctatgatt ttgctagcgc ttatctttat
     4201 tgtcagccta gatagcaatg attttctggt cgtgaccagc gctgtcttta gctgtttcaa
     4261 taatatcggg cctattctag gaaccacttc tagtttctca atctttagtc ccatctcaaa
     4321 aattctccta tcctttgcaa tgattgcagg gcgcttggag atttacccaa tcctccttct
     4381 ctttatgaag agaacttggt ctaagagata aaatacaaaa tccctctttt tacaattgtg
     4441 aaagagggat tcttatatta aaaaaggggg aacgttttta aatccaagcc tatctgtaag
     4501 caaaatcctt actcaaataa aagatggaaa aacttactac acagcttggt aatgctccaa
     4561 tacaaattgt aaggatttac ttggactctt tgttaaatga atatattcca ttcctctggt
     4621 agcaactatc tgaataccta atctttctgc atccaccttc atctctctat aataactccg
     4681 aacttgtgga gccagaataa ttaaatcata aacacccata atatcataat gagctccgta
     4741 cgctcctgaa ttcgcaatca ctctaacttc tgctaattga gcgccctcgt taattgcatt
     4801 ggctaattgc gcacttgttc cagaccctgc acaaagaacc agtactttca actccttact
     4861 ttttatttca tctaactcac caggcattat ttcacttgtc tcctcctcta aaataatatc
     4921 atttgagctt gcaacatctt ctttcatcag taactgtcta tcatacgctc tacagaatgg
     4981 caaataaatc aatatgtcga caactaaaat caaagataaa aatacaaagg agatagattg
     5041 aaaattcgtt ccaattaaca atcccaaggg accaggaaag gcccaaggta actggatata
     5101 aaatccattc attccaaaga aatcaataaa gacctttcct agaaatacat tcactggtgg
     5161 agtcatcaaa aaaggtacaa aaagataggg attcaaaata actggcatac caaatagaag
     5221 aggttcattc accgcaaata aaacaggagt aattgtagct ttacctaccg attttaattg
     5281 tttagaccgc ataaagaaaa tcaaaataaa tggtacaaca aaggtagccc ccgttcctcc
     5341 gatagcagcg atataatttc cgaaattagg cgttaaagaa tgataaggaa actgcccatt
     5401 tgcaagaagt tgagcattct cttccatatt gctcagttgt aaagctgtaa cagcaggtaa
     5461 gacaatagat ggtccatgaa ttccaacaaa ccaaagcaaa gcgcacataa accaaatcaa
     5521 catcatagca ggatatgatt ctgccccctt aaaaatagga gtcaatagtt gttgaaatac
     5581 ttgggcaaaa ggaacatcta aactaaaccg agatacaata tctaacaaac cactaataag
     5641 taaaacaaaa gaaaaaggaa aaatatctct aaaagcttgt gatatagccc caggaacttc
     5701 cttaggtaaa tgaatagtaa tgtctcgctt aatacagaat cggtaaacat ttacagtaat
     5761 aaaggtactg acaaacgaag ttatcaatcc ttgagatcct aaatagtttg tcgacaaact
     5821 tgtagatccc gttttctcat caactacaag cgttacagtt aatacaagat agccacatat
     5881 ggccgccaac attgcagaag tatcatttat tacctttcca ggaggcattt ttctattaac
     5941 atttccagcc aatgactttg caactgtccc tgaaacaata acacccaaaa ctcccatggt
     6001 tagcatatac actttcctca tccaggcaat agctggactc ggtaactcta ctccgaaatt
     6061 tttaggaatc ataataataa tcatcaaaaa gcttgaaaac ataattaaag gcatagtagc
     6121 aataaatcca tctttaatag ccatcatata attattagta gaaatttttt caaatttccc
     6181 ttgttgcttt tcaatggcag cgactaactt atccataaaa actcctcctg taaaaaattt
     6241 aagctcatag ttaaaatata ttaaacatca gtcaaatcgc taaccttaat tccgttttaa
     6301 tccttttacc caaagagcac ttttcttaat atatctcttc tgtgtttcat aatcaacaaa
     6361 gaaaagacca tatcgcttat tgtagccatt cgcccaagaa aattgatctt gcaaagacca
     6421 tataaaatag ccttgaatat tgactcccct atctcgagcc tctaaaactt tgtgtaaatg
     6481 ttgctcaata taacggattc ttttactgtc atcaataata tcattctcgc cctctggctt
     6541 aacctcttta agggctgtac cattttcagt taaataaatg accggatgtt gaggatattc
     6601 ttctttgata cgaagcaaca tatcaaataa cccttgagga taaatattcc aatcccaatc
     6661 tgttgtcgga ataccaggct tttttacaaa ttcacctaga gcatttaatt taaaagaaga
     6721 ctgtcctttt attcctgttg aattaaattc ttgtctattt tcacctttgt attcacgaat
     6781 aaattctgaa cgataataat tcatcccaaa catcgtatta agaggtgcag ctcttttcat
     6841 aatttctaaa tcaccatctt caataataaa gctagaatta ttagcttcca atatttgatt
     6901 caagtgaaaa agcgtgtcat cactgtagta gcccaaaaaa gttccatcta atagaaattt
     6961 attattataa acatcatacc gtttagctgc caaaatattt tctttttgcc catcaatagg
     7021 atagcctggc tttaaagcat gaatacaacc tacctttccc tcaatcccta attgatgaaa
     7081 ttcgaggact gcaagagcat gcgccaacaa catgttatga tttgcttgaa ttgcttcaga
     7141 taattgaaaa tggtgattcg gaggaaactg acctcctatg tattggcctg cagcaagaga
     7201 catcagttca ttgattgtaa accaatgctt gacttctgta aattcttgga aacaaaagcg
     7261 agcatatcgt atgaaacgat caatattctc tctattcaac caatcccctg tttctaacat
     7321 tttctgaggc gaatcaaaat gatgtaaaga aacaaacgga atcacattat attttaagca
     7381 ggactgaaag actctatggt aataacgaac agccaatata ttcggctccc catctatatc
     7441 aggaaatata cgaacccaag aaatagataa acgcaaagcc tgcaaaccat gttctgccgc
     7501 caaagctata tcctcttcgt aacgataata aaaatcacta gctggatctg gtaagaacgg
     7561 actattttct tgcaaataaa catcccacat atttattcct ttgccatcta ccctagttgc
     7621 accctctact tgataagcag cggtagcagc tcctaataaa aactccttcg gtaaggcttt
     7681 aacctgtaaa ttttccatga ctaatcctca ttgcaaacca atatgttctt caatctttcg
     7741 aattcgttca tattcatcaa taaaaaactt tacctgatca tacaatagca tcgttgtcat
     7801 caaagtatct tgaccatgaa tcatgataaa actcacttca aaattatctc cagatgcctc
     7861 ctccgctaat aaattagtct gttctcgatg ggcagacact attgagtcgt ttgcagactc
     7921 aaccaattct cttgcctgtt caaaatatcc atctctcgca aacgccaaag catccatcaa
     7981 atctgacctt gcatctcctg cgtaagcgac aattgtaaaa ccaagcatat ttatttcttc
     8041 ttttgatttc atctttcttc ctcctcaatc ttgaaccatc ttttacacat cattattaaa
     8101 atgacttcgc tacgatacaa atagaaagtc gaccctaaat atcttaaaaa tccttaaatt
     8161 tcataatatt taggatcaat cttctctgaa taccttggtt cattttctag acttatgatt
     8221 tataagtgga aaccagcgta attttttgct acttcaatta cacgataaac atccaaatta
     8281 gattggaata gcagttcgtg cccacggata taattcaaca catttctcca ttcatcttga
     8341 gcttgccttg tttttccagt tgcttgaaga tataaagcct tcgccatctt aacaacatat
     8401 tcacgatgat aactgagctg tttccattca tccttttgac tatttaataa cttagaaata
     8461 ttttcctcaa taattggtaa aaaattatca gctagattgt aagctatata ataatgattc
     8521 gctaaaacat cattttgacg actgccaatt gcattaaaat agtcacaaga ggaataaatg
     8581 gataattttt ctaaatattc aacaacagac tgccaatttt ccccatacaa agcaaaaaag
     8641 tattcttcaa tcaattcttc ataacttctt gtcttcttcc agagcatttg ccccatgaca
     8701 taattaggaa aattatgagg gaatccggca cgtaattctt gacacgaaat gtacccattc
     8761 aaatgtaggt ttgaaagata agatacatct ctgtaaatag tttgactaat tttcatatag
     8821 cctaaatcgc cataatgagc acgccctaaa ggatagtcat atacgaaact atctccttta
     8881 aatgtttttt gccactcaaa aagataagat aaattttcct caagagaatt cggaagtata
     8941 attttattac gcatataagg tttaggcgta ggtatggaat tgtcaaaatc tacatctgca
     9001 taactcattt caaatgttct tgtaatcggt gcaaacatca tggtaaagcg ttcaggatta
     9061 tctaattttt ctttctgagg cgcccataac aactcatggt aaagcagaaa acaaatcttt
     9121 gtatctaatc cctcactcgt taaagcccta tccaattgat tgagaatacg aatatactga
     9181 tccgaaacca attcttgtct acagttttcg cattcacaaa tattattacg agcatccgac
     9241 aaccatacat gtaagtagtt aacatcaggt cttttcttgg cataatcctt gataatttct
     9301 accatcttat cagctacatc tggatttgaa aaatccaggc tggttaaaat cggagccgta
     9361 ttaaacaatt ctcgtttccc gtttatttca gcgacatagg gtttcttctc ctctgaaata
     9421 ctaagacctg attcccagcc aaattttgaa gagtaaccta aaacctcacc tgtccatcca
     9481 tgaccaacac gatgatgaat aagacctctt ttttgcaatt ctttatccaa cctatcactc
     9541 aattcttgta ctaattcatt tgagaatttt tctttattta ggtatggatt aaactcatgt
     9601 tcataccaac gtttcaaaaa agagtaagga ttttcaaact ggatgaaaaa actgttcatc
     9661 ccaatcttag gtagccaatc aatgaaatct agtatatttt caaatgaatc cgctccctct
     9721 atacatactc ccctatgata gtaactggct gtttcatcta tagatagctg tttatctaaa
     9781 aaatcttcaa atcgtaactc tggaacaaag tcatgtctgc gccctggtct agtatacaca
     9841 accccaaatt catgaaacat tcggtaaatt ccaataagta aacctacaat tgtattagac
     9901 tcaattctcc ccttaccctt gtctaatgag ataagaacat ggtcttttct gtcctcttta
     9961 ttgactaatc ctacaaaaat atccgcttcc ttctcctcga caagctctat gttagtctta
    10021 aagacccgat tcatatagta acttaattcc tctaaggcaa agctagaata agtatttttc
    10081 tgttgatcaa atactctgat aaccatctta tttccctcca acagtagctt tttctttttc
    10141 aagattgcgt ttatctgcaa ttttgaaaaa tggataataa attgctacag aaaccaaaat
    10201 caaaacaact tgtaatagag ctcctcgcca actagctcct gtcgcaagga agcctcctat
    10261 gatcggtggc attgtccacg gaaggattac acctgttgta tagggtacta atcctacagc
    10321 cattgataca taggtaatca aggcattgat tgtaggagta gcaataaacg gaatcagcat
    10381 aatcggattt aaaactgttg gaaacgtaaa tagaatagct gtattgatat taaaaataga
    10441 cggtataata gctagcttac ctaatgtttt attcgcacga ctcttactaa ataggaagag
    10501 acaaatcgca agaccaatag tcgctccacc gccaccaata aatacaaata aatctttaaa
    10561 cggtaatgta ataatgtgtt ggagtgtttg tcctgcagcc acagcttctt ggttttctgt
    10621 agtaaattgt aaccaaactg ggtctacaaa agcatttaaa acttgtcccc cattaactcc
    10681 acagaaccaa aagaatgagt ttacaataac acatagaatc atacctggaa gcgttcctgc
    10741 aattaactta agcggtgttc caacaattgc acctaggagt ccgttgagac ctcctgcaac
    10801 tcctgccgct tcaagacctt ttaagaccag agcccacaaa acaaaagtag taaagccagg
    10861 taaaagagct gaaaatgatt tacttaccac atctgggaca ccacttggca tttttatcgt
    10921 aattccgcgc tggataaaca tacgataaat ctctgctgta accaatccaa tagtaatcgc
    10981 cataaacaaa gaagatgcat tcaggctaga aaatggtatt gcgccgccaa ataactgctt
    11041 gacctgctcc ccatttttat cataaaccat actcgaaaaa cgaggtgcca tcaatacaaa
    11101 actggataag gctatgatcc ctgccgacgg accatctgta ccatatcctt ccgacaacct
    11161 gtaggcaata ccgaaacaag cgaccaagga gataatcgct actgttgact gattcatagt
    11221 tgtcaagata cttagcaatc caacactatt cagccaattt gtaaaagcct cttgaggcca
    11281 actgattaga ataagaaata aagagccgac taaaatcaaa ggcattgcca tcataaatcc
    11341 ttcacgaata gctttcaaat gccgttggtt gccaactttt tcaccaaacg gagcgacttt
    11401 tgtttctaaa aacgctaaaa tattgttcat caccggaact cattttttta ttttttataa
    11461 attaaatata ccactaacag aaagcgttgt caatatattt tttgttgttt ttacaatatt
    11521 tttttctttg tatagaataa taattttaat tgattttttc ttatgtttaa tataaaataa
    11581 accaaaggag gaaacaaatg attacaaaac aatcagcctt tttacaaaat agagccacta
    11641 tactggagtt tctatatcga aatccagcaa catcccgtac agatattgtt aatgaaacag
    11701 gacttactcc agcaacaacg actaacatca taaaagagct aagcgaacag tccctcatat
    11761 acgaaactgg tgatgaattt agcgagtttt ccggatctgg aagacgtaga aaaactattt
    11821 ctatcacaga taatattccg tatgttgttg gaggtattga aataaacgtc ctaggtatct
    11881 ttctcagtct atgtgactta caagggaaaa ctcttttcga gacagaaatt ttgaatgaag
    11941 attatcctat ttcagaaatc aattccacca ttaccaatat gataaaaaca gctatagagt
    12001 acgtcccttt ggaaacaaaa ttacttggat ttggcttatc aatacctgga cattataaca
    12061 aagactccgg aagtatcatt acaaacaacc ccatatggga atcttttaat ttattaaatg
    12121 taattaaaag attcaatttt ccttttattg taaaaaataa tatcgattgt atggctatag
    12181 gacaatacct ttttaatcca cacaataccc ccgataactt tattttccta cacgctggat
    12241 taggtattta cacttccttt ttcacaaaag aaaaaatagg agcctctaaa aatccttata
    12301 tcggagaaat tggacacacc attgtcgaat tgaatgggca atattgtgaa tgcggaaaaa
    12361 aaggttgttt acaaacatat atttcggatg cttggttaat caaacatgcc caattattat
    12421 ttaaaaattc tcaactaact gtactaaaaa gccttgtaaa gactgaaaaa gacattcatt
    12481 tagataccct tttaacagct tacaacttag gcgactctgc tttacgccaa caaattgata
    12541 aaggagtcaa tttattggcc acttctattg caaatcttct cctcatcaat cctgctgata
    12601 aaatctatat caacagtcaa ttgcttaatt atcaaccttt cactcatgaa gtcagggata
    12661 aaatccaaga tcagctccac ttcgttccct ttactcgtaa tatagaaatt gaaattttac
    12721 cttacaacaa acatcgtgga agtataggag cttgtgcatt agctatcgtt gcttttttca
    12781 tagagcatag caatgtatta caagatatta tttcacctta atatatatta gaaatctata
    12841 gacctgttta aatcaactat aacctgtagt aaatatctcg tatttagaca atatgaaaac
    12901 aagacgactt ccatatagga aaccgccttc tcgctatgct gcatgaaact gtaaaaatag
    12961 atgaaattga tagcggtagt cagatcaaga aacatcgtaa ctctaaaaag tcacaatata
    13021 taagttcatc ctcggaaaaa tatcattcta attattgaaa tgcctacatg aaaagaaacg
    13081 ttaaatgctc ataaaacaac gaatacaggt atcaaaacta tgacaaaaca aatccctaaa
    13141 tttactaaag acactgctca actttacacc tgtaaatggt tgttgtataa taaagttaca
    13201 aagatgtacg accacactat tgtaaatcat agtgttcgcg aatatattac tgatagcatt
    13261 tctacaaata caattgaaga ggctgaatag gctttaaacg aaatatgtca gtgttttggc
    13321 attcctagcc ttcatatcat ttaaagaatt gtatagataa aattttttcc aatacagaca
    13381 ctcgtaacaa ctgcttcatt tttctaccaa catatttagg aacaggataa gatacaagag
    13441 gattaatcca tagctcagtt ctataccaat ctaagacaaa taagctaaaa aaacgattga
    13501 taataagcaa atagattcca aattttctct atctgctcat tttaataaac aatactagtg
    13561 taactatcct tccagtcaga agcttgtcaa atcacaccga aaattcttct aaaatttatc
    13621 tcgttaggca atcaagcaaa aactcgacga tagtacaaac attatcatac agaattgact
    13681 tcctaaatta tatactttag taaggttttc ggataagaaa aaaggttcat tttacatttc
    13741 taaacattct tttctaagat gaaaaacaga atttttcgat tgtaatttaa agcaacaaga
    13801 agattttcag tatcatccta tagatacgag ctaattaaga aaaactacat ttttgaatat
    13861 aaactacaat aatataaact aaattccata ggaggaagac aatggattgg tacgattata
    13921 tgatacaggc atccaaacaa tcacaattca acgcaagcca ttggtttcgc tatttgcgaa
    13981 aagttatttt tgaagactat tcttatttaa caaaccaaga tgtagaaaag ttgctagact
    14041 ccaaagaact aacccgtttt caaaaaatta gcttgaagta tgcctttcaa gagcatactc
    14101 caactcataa atatgtgatt tcattaaata aacctgctaa gttaaccaat gttcaaaaat
    14161 tgatggagaa atacaaacat ggataaaatg aaaccggtct tccaagccct aaataagaaa
    14221 ttaattcagg aaaatctgac tttaacaatt atggttatgt cttagaatat catggtttac
    14281 gtgccacaca agatgttgat gctttttatg accaaaatca gaaaatcaat gaaattattg
    14341 ctcgtgtagg aaaacagttt aatcttaaca tccacgaaga gttatggctc aacaatcatg
    14401 ttgcaaagca aatatgaaca aacaacctcc cttaagttta tgcgaatcct tgtactcatt
    14461 tgagaatctg accgttttag tagtccctat tgagtatgtg ttaggaatga agatgatgag
    14521 tatcagagag caggatttga aagatattgg tgcaattatt aaatacaaaa actttcattc
    14581 accattcgat acctttaaat atttaaaaga catgggattt gatactattg acctctctgt
    14641 tctattagaa ggttttagtt atgcttatgg tatggattgg ttagaaaaat tttttaaaga
    14701 aaatcaagat aaacttcgcg aattttatta acagtatcaa acaagatgat tgtcaattag
    14761 ccagtcgtct tgtttttatg atggaatgaa ataaaatcgg aacaaatcac ttagagaatt
    14821 ccagacaatt tctagcactg tttgagacgt ttcagcggac tatagcaaaa tgaaataaga
    14881 ataagacaaa tcgatcagga cagtcaaatc gatttctaag aatgttttag aagtaaaggt
    14941 gtactattct aatttcaatc tactatattt cagattcggt ccactattcc ttcccaaaaa
    15001 caggctctat aatatttgta gtgggtaaat ccact
//
LOCUS       DAVBCW010000044        14733 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVBCW010000044 DAVBCW010000000
VERSION     DAVBCW010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14733
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.044"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            244..1110
                     /locus_tag="VQ514_001149"
     CDS             244..1110
                     /locus_tag="VQ514_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2499907.1"
                     /translation="MGTLLATRLKNRRKELKMSQRELAEGICKQGQISRLESGEFTPG
                     ADFLHALAKKLKVSMDYFFDEQVVEKVEELSEFKKLAQTFITNRNYESLKYIYELENV
                     KAHRLSLADKIYMEWIKSLIDFYFYGQKEDAIVRLEGVLSQLSVSDLNYLQVSNTLFN
                     FYYDIGDLGRFNKIREKLEYQVNHLNLSTIEELELSIKFNYNVCRYLWLQKNIEEAIT
                     KITATIKQCKAYRTNYLLADLYLLMGNVSENFSSKSSVKEYFETAYFLYKLEENMSMT
                     LKVEHYIADITE"
     gene            1447..2004
                     /locus_tag="VQ514_001150"
     CDS             1447..2004
                     /locus_tag="VQ514_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase family
                     flavoprotein"
                     /protein_id="HET2499908.1"
                     /translation="MDKKNLLVLVTGSVGASNVDTYLYYIKKFYNVKVILSENSKKFI
                     SKELISYFCDKVYDEIFVEEVVPHVFLPYESDLLLILPATANVIGKIANGIADDLVTA
                     TVLNFNKKIIFCPNMNSTMWDNHIVQRNVSILKELGHIFLFESKKTYEVGLRKAIDST
                     CSMLQPQSLVKELIKLENIVLEEGH"
     gene            2225..3331
                     /locus_tag="VQ514_001151"
     CDS             2225..3331
                     /locus_tag="VQ514_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HET2499909.1"
                     /translation="MINKKIQQVVLESLQNFLNGNFISPCVVYDFGLLETVLDEFKNQ
                     IPVTFNYQLFYAVKANSNEKILEFLVDKIDGVDVASLSELDVAKKFFPPTQISVNGPA
                     FSYETLYNLIKKQYKVDINFLEHLQQFSPKESVGIRVTEPDELNNRMSRFGINICSDN
                     WTSNLQNPLITRLHFHFGEKDDKFIVKLDKILFKLQEINKLREVREINLGGGFMKLFM
                     ENRLKEFFLSLMEIYKKYDIDSTVTTIIEPGSAITSFSAYMITSPVNVSEVNEQQVIT
                     LDTSIYTNTLWFVPHIITTLNSSSKERYSTILYGNTCYEHDKYKMKVSLPRLTQNSSI
                     VFFPVGAYIKSNHSNLHRNDFMREVYLWTKNLTY"
     gene            3307..4698
                     /locus_tag="VQ514_001152"
     CDS             3307..4698
                     /locus_tag="VQ514_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499910.1"
                     /translation="MDKKLDILDKVKEYLGNKTTQILDNQYKEFLKLNDIRRAFGISE
                     KVLNNSFNFTSKEFNDLINNENYLFEYACRIREEWRKKCFNHSYRFLCSPIITDDFLN
                     TKTLRSSQIEYKYERYLSKSSIGDRAVDGFVSFNTLTANGMSAIKLCLEILNSIFFKK
                     KIDLLYSTGYYETRFLLNNLAKSGISCYEVSNCELDKDKFYNVFMMEPNRADLTLQKT
                     DFKIVEYFVKYKNNSIKVVILDISYQGSNFKLVEFLEKFKFANVIIFVVRSLIKLDQM
                     GLELTNGGIIEVFIPNHLRKLKNFIEEEFNKFRNSHGANLSLYEYCLLDNSLTLKNDW
                     NYSDLVMKFTSNFYADIKDLFMENSDIEIIHEEGVPFVFLDLIGEGKKEYEMFFQWLN
                     FFYKQLGITLYARNSFGFRNLTVEYFGIIGTERYIFKICPGVYKGLSYYLMKFLLKSF
                     SNEYLKTTDEVNR"
     gene            4695..5873
                     /locus_tag="VQ514_001153"
     CDS             4695..5873
                     /locus_tag="VQ514_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2499911.1"
                     /translation="MKNLIKLLIIRLIVNLADSVFYIVALWHVSNNYSSSMFLGIFIA
                     VNYLPDLLLIFFGPVIDRVNPQKILIISILVQLAVAVIFLLLLNQISFWVIMSLVFIS
                     VMASSISYVIEDVLIPQVVEYDKIVFANSLFSISYKVLDSIFNSFASFLQVAVGFILL
                     VKIDIGIFLLALFILLLLKFRTSNANIENFSFKYYKREVLQGTKFILNNKLLFKTSIS
                     LTLINFFYSFQTVVVPIFSIRYFDGPIFYGIFLTIAGLGGILGNMLAPIVIKYLKSNQ
                     IVGVFLFLNGSSWLVAIVIKDYTLSLILFFVCFMSKGVFNIIFNSLYQQIPPHQLLGR
                     VNTTIDSIISFGMPIGSLVAGTLIDLNIELVLIAISIPYFLFSYIFYTDNGLKEFSIY
                     "
     gene            6298..6531
                     /locus_tag="VQ514_001154"
     CDS             6298..6531
                     /locus_tag="VQ514_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2499912.1"
                     /translation="MISIDLGGTNIKITVLSNDGEIETLWSITTDTSEKGSQIISDII
                     SSIKNKLTERNIPDSDLLGIGMGSCSSYFPCKS"
     gene            6814..8763
                     /gene="mutL"
                     /locus_tag="VQ514_001155"
     CDS             6814..8763
                     /gene="mutL"
                     /locus_tag="VQ514_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HET2499913.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKIQITDNGYGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(9088..9555)
                     /locus_tag="VQ514_001156"
     CDS             complement(9088..9555)
                     /locus_tag="VQ514_001156"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HET2499914.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(9556..10761)
                     /locus_tag="VQ514_001157"
     CDS             complement(9556..10761)
                     /locus_tag="VQ514_001157"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HET2499915.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(10781..11416)
                     /gene="ribE"
                     /locus_tag="VQ514_001158"
     CDS             complement(10781..11416)
                     /gene="ribE"
                     /locus_tag="VQ514_001158"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HET2499916.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(11401..12501)
                     /gene="ribD"
                     /locus_tag="VQ514_001159"
     CDS             complement(11401..12501)
                     /gene="ribD"
                     /locus_tag="VQ514_001159"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HET2499917.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHHVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(12636..12750)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            12907..13500
                     /gene="ruvA"
                     /locus_tag="VQ514_001160"
     CDS             12907..13500
                     /gene="ruvA"
                     /locus_tag="VQ514_001160"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HET2499918.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDGLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            13510..14073
                     /locus_tag="VQ514_001161"
     CDS             13510..14073
                     /locus_tag="VQ514_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HET2499919.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKIFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRDFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            14070..>14733
                     /locus_tag="VQ514_001162"
     CDS             14070..>14733
                     /locus_tag="VQ514_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2499920.1"
                     /translation="MMSNKNKGILIFAILYTVLFVFDGVKLLASLMPSAIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVGL
                     VGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLPGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAI"
BASE COUNT         4636 a         2397 c         2761 g         4939 t
ORIGIN      
        1 gaaaggggct gaaagaacta ggggaacagt tgtaattaga aggtgatagg tatgaaaagt
       61 ggcgttgaag ctggataagt ttataaagag tttgaatgaa gaaatgaaaa gtaaataagt
      121 tgatgatgaa ttgcaaaaat agattttata tacaatttgt atctttcaaa tcattgattt
      181 gaattttaga tttaatcatg ttacaattaa aaatgaaatc aaatagaaga ggaagatgca
      241 aacgtgggca cactattagc aacaagatta aaaaatagac gaaaagaatt aaaaatgtct
      301 cagcgagaac tagctgaagg tatctgtaaa caggggcaaa tcagtcgatt agagagcgga
      361 gagtttactc ctggagcaga tttcttgcat gcccttgcta agaagttaaa agtcagtatg
      421 gattattttt ttgatgagca ggttgtagag aaagttgagg agttatcaga atttaagaaa
      481 ttagcacaga catttattac aaatcgcaat tatgagtctt tgaaatatat atatgaattg
      541 gagaatgtaa aagctcaccg cctatcttta gcagataaaa tctacatgga atggataaaa
      601 tctctgatag atttttattt ttacggacag aaagaagatg cgattgtacg attagagggg
      661 gtattatcac agttaagtgt atctgatttg aactatctcc aagtttcaaa caccctattc
      721 aatttttatt atgatattgg agatttaggt cgtttcaata aaattcgtga aaaactagag
      781 tatcaagtaa atcacctcaa tttgagtaca atcgaagagt tagaactatc tattaagttt
      841 aactacaacg tttgtcgtta tctatggtta cagaaaaata ttgaagaagc tattactaaa
      901 attacagcta ctataaaaca atgtaaggct tacagaacga attatctttt ggctgatttg
      961 tacttattga tgggaaatgt aagtgaaaat ttttcttcta aaagttcggt gaaagaatat
     1021 tttgagacgg catattttct atacaaactt gaggagaaca tgtcaatgac tctgaaagtt
     1081 gagcattaca ttgcagatat aacagaataa tgaatacggt gttcaagtga gcatcgtttt
     1141 tcaaacctag ttcagataca aaaaagtatc aaataaaaaa aaataaaaaa acattataaa
     1201 aacaattgac aagctaattt tgaagtgata aaataaatgc aagaaaacaa aaggagaggt
     1261 atatgagaag tttgtttaag aaaatagttg ctgtattagt tattggctta attctgttag
     1321 gaattgcgag aacacctcaa gttcataaga tggctagagg gattgatcct ggtcctgcga
     1381 attttattta gaaatcaatt tctactaatt gagtactaat tgagtattaa tgggtaaaaa
     1441 gattgtatgg ataagaaaaa tttacttgta ttggttactg gttcggtagg tgccagtaat
     1501 gtagatacat acttatatta tataaaaaaa ttctataatg ttaaagtgat actttctgaa
     1561 aattctaaaa aatttataag taaggaatta attagttatt tttgtgataa agtttatgat
     1621 gaaatatttg ttgaagaggt agttccgcac gtttttctgc catatgaatc tgacttactt
     1681 cttattttac cagctacggc aaatgtgatt ggcaaaattg ctaatggtat tgctgatgat
     1741 ttagttacag caactgtttt aaactttaat aaaaaaataa ttttttgtcc caatatgaac
     1801 tctactatgt gggacaatca catagttcaa agaaatgtat caattctaaa ggagttggga
     1861 catatatttt tatttgagtc taaaaaaaca tatgaggtag gattgcgtaa agcaatagat
     1921 tcaacatgtt caatgttaca accacagtcg ttagtaaaag aacttatcaa attagaaaat
     1981 attgtccttg aagagggaca ttaaaaacta ctgagaatat taaggagggg aaaaaatgga
     2041 aaattcatca atcgatgtag atatgctgtt ggaagaattg acacaagaag caatggtcgt
     2101 tgttgctgtt gataaggact gttaatttaa acttatggca atatatgaaa ggttactgga
     2161 tgttttaaat tatgcaggca gtagcctttt attatataca aatggataaa gtaaggataa
     2221 tacaatgatt aataaaaaaa tacaacaagt tgttttggaa tcattacaga attttttgaa
     2281 tgggaacttc atttcgcctt gtgtagtcta tgattttggc ttgctggaaa ctgtacttga
     2341 tgaatttaaa aatcaaattc ctgtaacatt caattaccaa cttttttatg ccgttaaagc
     2401 aaattcaaat gagaagatac ttgaattctt agtagataaa attgatggag ttgatgtggc
     2461 gtcattatct gaattagatg tggctaaaaa atttttccca ccaactcaaa tttctgttaa
     2521 tggtcccgca ttttcttatg aaactttata taatctgatt aaaaaacaat ataaagttga
     2581 tattaacttt ttggaacatc ttcaacaatt ttccccaaaa gaatctgttg gaataagagt
     2641 aacggagcca gatgaactta ataatcgtat gagtcgattt ggaataaata tttgcagtga
     2701 taattggact agtaatttac aaaatccttt aattacacga ctgcattttc attttggaga
     2761 aaaagatgat aaatttattg ttaagttaga taaaatatta tttaagttac aagaaattaa
     2821 taaacttaga gaggttagag aaataaatct tggaggcggt tttatgaaat tatttatgga
     2881 aaatcgtttg aaagaatttt ttctatcact tatggaaatc tataaaaagt acgatattga
     2941 tagtactgtg actacaataa tagaaccagg tagtgcaatt acttcatttt ctgcctatat
     3001 gattactagc ccagttaatg ttagtgaggt gaatgagcag caggttatca cgttagacac
     3061 atcaatatac accaatacat tatggtttgt tccgcatatt attacaacgt taaattcaag
     3121 tagtaaagag cgttatagta ctattctcta tggtaatacc tgttatgaac atgacaagta
     3181 taaaatgaaa gtttcgcttc caaggttaac tcaaaatagc agtatagtgt tttttcctgt
     3241 aggagcttat ataaaaagca atcattcaaa tttacatcgt aatgatttta tgcgggaggt
     3301 atatttgtgg acaaaaaact tgacatatta gataaagtta aggaatattt aggaaataaa
     3361 actactcaaa ttctggataa tcaatataaa gaatttttga aacttaatga tataaggcga
     3421 gcgtttggta tttcagaaaa agtattaaac aattctttta attttacgag taaagaattt
     3481 aatgatttaa ttaataacga aaattattta ttcgaatatg catgtagaat tagagaggaa
     3541 tggagaaaaa aatgctttaa tcattcttat cgttttctat gctcacctat aattacagat
     3601 gattttctta acacgaagac attgagaagt agccaaattg aatataaata tgagcgatat
     3661 ttatcgaaaa gttcgatagg cgatagagcg gttgatggct ttgtttcctt caatacttta
     3721 acagctaatg gtatgtctgc tattaaacta tgtcttgaga tattaaactc tattttcttc
     3781 aagaagaaga ttgatttatt atattcaacc ggatattatg aaacaagatt tttattaaat
     3841 aatcttgcta aatcaggtat tagttgctat gaggtaagta attgtgaatt ggataaagat
     3901 aaattttata atgtattcat gatggaaccc aatcgagccg atttaacatt acaaaaaact
     3961 gatttcaaga tagtagaata ttttgttaag tataaaaata attcaataaa agtcgttatt
     4021 ttagatattt catatcaagg ttctaatttt aaattagtag aatttttaga gaaatttaaa
     4081 tttgcgaatg taattatttt tgtggtacga tctttgataa aattagatca aatgggatta
     4141 gaattgacaa atgggggaat aatagaagtg tttattccta atcatttgag aaagttgaaa
     4201 aattttattg aagaggaatt caataaattt agaaattctc acggagctaa tctaagcctc
     4261 tatgaatact gtttgcttga taattcttta accttaaaaa atgattggaa ctattctgat
     4321 ttagttatga aatttacgag taatttttat gctgatataa aagacttgtt catggaaaat
     4381 tctgatattg aaatcatcca tgaagaggga gtaccttttg tatttttaga tttaataggt
     4441 gaaggtaaaa aagaatatga aatgtttttt caatggttaa acttctttta caaacagctt
     4501 ggaatcacat tgtatgctag aaatagtttt gggtttcgga atctaacagt agagtatttt
     4561 ggaattattg ggacagaaag atatatattt aagatttgtc caggtgttta taaagggtta
     4621 agttattatt tgatgaaatt tttattaaaa tctttttcaa atgaatattt aaaaactact
     4681 gatgaggtta atagatgaaa aatttgataa agttgctaat aattagattg attgttaact
     4741 tagcagacag tgtattttat atagtagcat tgtggcacgt tagcaataat tattcttcga
     4801 gcatgttctt aggaatattt attgcagtaa attatctacc ggatttgtta ctaatctttt
     4861 ttggaccagt tattgacaga gtaaatccgc aaaaaattct tataatatca attttggttc
     4921 aattagcagt ggctgtaata tttttattat tattaaacca aatatcattt tgggtgataa
     4981 tgagtctagt gtttatttca gtaatggcta gctccataag ttacgtgata gaagatgtgt
     5041 tgattcctca agtggtagaa tatgataaga ttgtatttgc aaattctctt tttagtattt
     5101 cgtataaagt attagattct atttttaatt cattcgcatc atttttacag gtggcagtag
     5161 gatttatttt attggttaag atagatatag gcatattttt acttgctcta tttatattgt
     5221 tgttgttaaa atttagaact agcaatgcga atatagaaaa cttctctttc aaatattaca
     5281 agagagaagt gttgcaaggt acaaagttta ttttaaataa taaattatta tttaaaacca
     5341 gtatttcttt aacgcttata aacttttttt attcatttca gacagtagtt gtaccgattt
     5401 tttctattcg atattttgat ggtccgattt tttatggtat ttttttaact attgctggtt
     5461 tgggtggtat attgggaaat atgctagcgc caatcgtaat aaaatattta aaatcgaatc
     5521 aaattgttgg tgtatttctt tttttgaacg gctcaagttg gttagtagca attgttataa
     5581 aagactatac tttatcactt attttatttt tcgtttgttt tatgtctaaa ggagtcttca
     5641 atattatttt taattcgttg taccaacaaa tacctccaca tcaacttctt ggtagggtaa
     5701 atactaccat tgattctatt atttcttttg gaatgccaat tggtagttta gttgcaggaa
     5761 cgcttattga tttgaatatt gaattagtgt taattgctat tagcatacct tattttttgt
     5821 tttcttatat tttttatacg gataatggat tgaaagaatt tagtatatat tagaaatgtt
     5881 tatgttcatt caaaagcata atgactataa ctgaaaaaga aaagtgatat ctttaaggtt
     5941 gttcttcttg gtggtgagat tcgtgagaca acccaagctt ttgtcggaaa gattaccaat
     6001 gctttgatgg ataggatgta ctttagcaag atgtttttag tggtaacggt atcgtggatg
     6061 gacgtgtaat aacctcttct ttcgaggagt attttactaa aaaactagcc ttggagcgtt
     6121 ccccagaaac ggacttactc attgactctt caaagatttg gggagaagat tttgcttcat
     6181 ctgttccttg aaaaaagtca cagcagtcat cacagacgat agtactgaac aaaactatga
     6241 agagttagaa atttatacgc aggtgattgt ataaaggatc tggaaataga taagaagttg
     6301 attagtattg acctaggtgg tacaaatatt aagattactg ttctttcaaa tgacggtgag
     6361 attgaaactt tgtggagtat tacaacagat acaagtgaga aaggttctca aattatatcg
     6421 gacatcatca gttctattaa aaataaattg accgaacgga atattcctga tagcgacctt
     6481 cttggaatcg gtatgggaag ttgctcatca tactttcctt gtaaatcata ggggctataa
     6541 actctccgtc tacttgtcct gcaacaattg aagtctgctc aaaacgccgt ccgctaatct
     6601 tttcatagac tttctccctt ttaggagcct agctttctag tttgttcttt gatttttatt
     6661 gagtatacca ctattttact ccctctggca agggactttg tctatgtgga gggattgggc
     6721 tcctatgtgg tggagctttt ctgttctttc tgaaatatgg tataatagca ctaatcaatt
     6781 tctaggaaaa tagatacgga aaggggctga aagatgtctc atattattga attgccagag
     6841 atgctggcaa accaaatcgc agctggagag gtcattgaac gtcctgccag cgtggtcaaa
     6901 gaattggtag aaaatgccat tgacgcgggc tctagtcaga ttatcattga gattgaggaa
     6961 gctggtctca agaaaatcca aatcacagat aacggttatg gaattgccca cgatgaggtg
     7021 gagttggccc tgcgtcgcca tgcgaccagt aagataaaaa atcaagcaga tctctttcgg
     7081 attcggacgc ttggttttcg tggtgaagcc ttgccttcta ttgcgtctgt tagtgtcttg
     7141 actctgttaa cggcggtgga tggtgctagt catggaacca agttagtcgc gcgtgggggt
     7201 gaagttgagg aagtcatccc agcgactagt cctgtgggaa ccaaggtttg tgtggaggat
     7261 ctctttttca acacgcctgc ccgtctcaag tatatgaaga gccagcaagc ggagttgtct
     7321 catatcattg atattgtcaa ccgtctgggc ttggcccatc ctgagatttc ttttagcttg
     7381 attagtgatg gcaaggaaat gacgcggaca gcagggactg gtcaattgcg ccaagcaatc
     7441 gcagggattt acggtttggt cagtgccaag aagatgattg aaattgagaa ctctgaccta
     7501 gatttcgaaa tttcaggttt tgtgtccttg cctgagttga ctcgggctaa ccgcaattat
     7561 atcagcctct tcatcaatgg tcgttatatc aagaacttcc tgctcaatcg tgctattttg
     7621 gatggtttcg gaagcaagct tatggttgga cgttttccac tggctgtcat tcacatccat
     7681 atcgaccctt atctagcgga tgtcaatgtg catccaacta agcaagaggt gcggatttcc
     7741 aaggaaaaag aactgatgac cctggtttca gaagctattg caaatagtct caaggaacaa
     7801 accttgattc cagatgcctt ggaaaatctt gccaaatcga ctgtgcgcaa tcgtgagaag
     7861 gtggagcaaa ctattctccc actcaaagaa aatacgctct actatgagaa aactgagccg
     7921 tcaagaccta gtcaaactga agtagctgat tatcaggtag aattgactga tgaagggcag
     7981 gatttgaccc tgtttgccaa ggaaaccttg gaccgattga ccaagccagc aaaactgcat
     8041 tttgcagaga gaaagcctgc taactacgac cagctagacc atccagagtt agatcttgct
     8101 agcatcgata aggcttatga caaactggag cgagaagaag catccagctt cccagagttg
     8161 gagtttttcg gacaaatgca cgggacttat ctctttgccc aagggcgaga tggactttac
     8221 atcatagatc agcacgctgc tcaggaacgg gtcaagtacg aggagtaccg tgaaagcatt
     8281 ggcaatgttg accagagcca gcagcaactc ctagtgccct atatctttga atttcctgcg
     8341 gatgatgccc tgcgtctcaa ggaaagaatg cctctcttag aggaagtggg cgtctttcta
     8401 gcagagtacg gagaaaatca atttattcta cgtgaacatc ctatttggat ggcagaagaa
     8461 gagattgaat caggcatcta tgagatgtgc gacatgctcc ttttgaccaa ggaagtttct
     8521 atcaagaaat accgagcaga gctggctatc atgatgtctt gcaagcgatc tatcaaggcc
     8581 aatcatcgta ttgatgatca ttcagctaga caactcctct atcagctttc tcaatgtgac
     8641 aatccctata actgtcctca cggacgtcct gttttggtgc attttaccaa gtcggatatg
     8701 gaaaagatgt tccgacgtat tcaggaaaat cacaccagtc tccgtgagtt ggggaaatat
     8761 taaaagtata aaaaagtctg ggaaaaattt tcaaaatcag aaaaacgcat aaaatcaggt
     8821 gttcaaaaac cttgatttta tgcgttttat catggaaata gttacttcat gttttcctaa
     8881 ttcttttcga aactcttttt aaaccacgtc agttttatca gtaatctcaa aacagtgttt
     8941 tgagctaatt ttgccagttt tatctgtaac atcgaagttg tgtttgacca ctctgcgact
     9001 ggtttcctag tttgctctat gattttcaca gagtattaaa ttgcgatttt gccaagtttc
     9061 tttattcgtc taaaagtaga gtctgttcta tgcgtctaat gtacgaatta ggttgaccat
     9121 ttcaatagct ccttgtgcac actcagaacc cttatttcct gctttggtac cagctcgttc
     9181 tatggcttgt tcaattgtat ctgtcgttag cacaccaaac ataacaggaa tttcgctatt
     9241 taaactgatt tgggcgattc ccttagatac ctcgctacat acataatcat aatgacttgt
     9301 attccctcta atgacagctc ccaagcagat aattgcatca tattttttac tttttgccat
     9361 ttttgatgca atcagtggta tttcaaaagc tcctggaacc caggctacct ctatatcttt
     9421 ctcgtttaca ttctctcttt tgagattatc tagtgctcca gataataatt ttgaagttat
     9481 aaattcatta aatctcgcta caacaatacc tattttaata ttgtttgcta ctaaattacc
     9541 ttcataagtg ttcatttatt tttcctccat atttaaaatg tgacccattc gatttttctt
     9601 tgtttctaaa taaaaactat cgtaaggatt ggcttctatt tcgattgata ttctactgga
     9661 aatggtaatt ccatattttt ctaactgttc aaccttgtca ggattatttg tcagtaaatg
     9721 aagtgactga agtcccagat ctttaagcat ttgtgctcca atatgatatt ctcttaaatc
     9781 accttcaaag cctaatgcaa gattggcatc aagcgtatcc atgccttgat cttgtaaatg
     9841 ataggctttt aatttattga taagtccaat tcctcgtccc tcctgtcgca agtaaagtaa
     9901 gacacccgaa ccattctcaa caatcatttt catagcttta tcgaattgct gtccacaatc
     9961 gcaacgtaaa gagcctaaaa catctcctgt taaacattcg gagtggaccc gacataatac
    10021 attggcttca tcctctatat ttcccataat aagagcaaga tgatgttccc catttagttt
    10081 atctatatag ctaattgctt tgaaattacc gtatctagta ggcatattga cagttgaaac
    10141 tcgttctacc agctgatcat atacttttct atattcttgt aattctttga tggtaattag
    10201 tggaatgttg tgtttcttcg agaactgaat taaatcatct gttctcatca ttttgccatc
    10261 atgattcatt atttcacaac ataggccaca ctcttttagt ccagctaatt ttaataaatc
    10321 aacagttgct tctgtgtgtc catttctttc tagaacacca cctttttttg caattaaagg
    10381 aaacatgtgt cctggcctgc gaaaatcaga gggtgttata tcttcagcta cacacatacg
    10441 tgcggtcagt cctctctcct cggcagaaat acctgtggtc gtttctttat aatcaattga
    10501 aactgtaaaa gcagtcttat gattatctgt attgttttca accataggtg aaagcattaa
    10561 ttgattagct aaactttcgc tcataggcat acaaattaat cctttggcat aagtagccat
    10621 aaaattaaca ttttctgttg tagctgcttg tgcagaacaa attaagtctc cttcattttc
    10681 tctatccttg tcgtctataa caagaacaag tcgtcccttc tgcaatgctt ctaatgcttc
    10741 ttgtattttt cgatattcca ttgactgatt atcctttctg ctaaaatcca ttttgatata
    10801 atagttcctt agatatttct gattttggag agttatccat cagtttttgc acatatttac
    10861 ctaagatatc attttcaaga tttactgtac tcccgacttg tttactctta agaatggttt
    10921 gttccaaggt atgagggata acagatactg aaaagtttac tttggagact ttagcgacag
    10981 tcagactaat gccgtcaatt gtaatagatc ctttttcaac tattaaatct aaaatttctt
    11041 tttgtgtgtt gatttgatac catacagcat tatcatcttt ttttattgac gagatttttc
    11101 ctgtaccatc aatgtgtcct gtaacgacgt gacccccaag tcgaccgttg acagataagg
    11161 ctctttctag attcacctca cttccatgtt ttaatagagt aagagctgtt cgactccatg
    11221 tttcattcat tacatcaact gtaaaggatt gatgattgaa atgagtaact gtaagacaga
    11281 taccatttac tgctatacta tcgcctaaat ggatatccgt taatattttt gaggctttaa
    11341 ttgatagttt acaattacga gagtctttct gtattctttc aacttttccg atttcttcaa
    11401 ttattcctgt gaacatggat aaatcacttc actttctatg agatagtcat ttcctatttg
    11461 agaaaaagca taaggtttca atctaatagc gtcatttggc aaagaaatgc cttcacctcc
    11521 gacaggaaac ttggcactgc ctccaaaaat ttttggtgca atatatattt tcagctcatc
    11581 aacaatttgt tgttccaaag cactccaatt cattagactg cccccttcta gaactaggct
    11641 atcaatctgc atgtttccta gatgttgcat taaactcgat aagtctatat gattgccttt
    11701 tttctttatg gaaagtattt cacagccatg attttgatat agcttcattt tatttttgtc
    11761 ttcagaggaa gtggcaatgt aagttttaat atcatttgct gtttttacga ttttagaggt
    11821 aagaggagtt cgtaaatgtg tatcgcatat gatacggata ggatttttcc cttcctccaa
    11881 tctacatgtc agcaaaggat cgtcttgaat aacagtattg actcccacca taattgcact
    11941 aacatggtgt cgtaactgat gcacatgctt tcttgcttct tcttcagtaa tccatttgga
    12001 ttgatttgtt ttagtggcta tttttccatc cattgacatt gcatatttca taaaaacata
    12061 gggtacatgc tgggtaatat actttctaaa actttttatt aagttaagac actcattttc
    12121 taaaattcca acagtaactt gaagattatt ttcctcaagt atctttactc cttttccaga
    12181 tacaatagga ttacagtcta ggcttccaat gactactctt gtaataccac tatcgattat
    12241 agcatctata cagggaggtg ttttcccgaa gtgacaacag ggttcaagtg ttacataaag
    12301 cgtcgctccg acaggggatt ctctacagtt tttaagagca tttctctcag catgtgggcc
    12361 accaaaaaac tcatgataac cttgtccgat gatgtgatta tcttttacaa taactgcgcc
    12421 gaccatagga ttgggattga cgtaaccagc ccctttttgt gccagtttta ttgctaattt
    12481 catatatttt gaatcgctca tctcactacc tccaaaaaaa atataccttg aataggggac
    12541 tactcaaggc atacaaaaga aaacttatgc gattaacaaa aatactctga aatgacaagt
    12601 aatcatttca gagcacgcaa aaagcacaaa tatactttta tcttctttca tccagactat
    12661 actgtcggct ttggaatttc accaaatcat gcctttcggc tcgtgggcta taccaccggt
    12721 agggaattac accctgccct gaagatagtt attcaattac agatgattat agtacttaat
    12781 tttgaatatg tcaacagata aataccgatt gtttttgata tactgtattt gtgataatcg
    12841 attctcgctc ctcggataaa gaaaatatga tatactagat aaacgaaata agagagaagg
    12901 aatactatgt acgcatattt aaaaggaatc attaccaaaa ttactgccaa atacattgtt
    12961 cttgaaacca atggtattgg ttatatcctg catgtggcca atccttatgc ctattcaggt
    13021 caggttaatc aggaggctca gatttatgtg catcaggttg tgcgtgagga cgcccatttg
    13081 ctttatggat ttcgctcaga ggatgagaaa aagctctttc ttagtctgat ttcggtctct
    13141 gggattggtc ctgtatcagc tcttgctatt atcgctgctg atgacaatgc tggcttggtt
    13201 caagccattg aaaccaagaa catcacctac ttgaccaagt tccctaaaat tggcaagaaa
    13261 acagcccagc agatggtgct ggacttggaa ggcaaggtag tagttgcagg agatggcctt
    13321 cctgccaagg tcgcagtgca agcaagtgct gaaaaccaag aattggaaga agctatggaa
    13381 gccatgttgg ctctgggcta caaggcaaca gagctcaaga aaatcaagaa attctttgaa
    13441 ggaacgacag atacagctga gaactatatc aagtcggccc ttaaaatgtt ggtcaaatag
    13501 gagcagagaa tgacaaaacg ttgttcgtgg gtcaagatga ccaacccact ctacatcgcc
    13561 tatcatgatg aggagtgggg ccagcccctc catgatgacc aagcgttgtt tgagttattg
    13621 tgtatggaaa cctatcaggc aggcctgtct tgggaaacgg tactcaacaa acgccaagct
    13681 ttccgagaag cctttcatag ctatcaaatt cactcagtcg cagagatgac tgacactgaa
    13741 ttggaagcca tgctggagaa tccagctatc attcgaaata gagccaagat ttttgctaca
    13801 cgcgctaacg cccaagcttt tctacagtta caggcagagt acggctcttt tgatgcctat
    13861 ctttggtctt ttgttgaggg gaaaactgtc gttaacgatg ttcctgatta tcgccaagcc
    13921 ccagctaaaa cacccttatc tgagaaatta gccaaagatc tcaaaaaacg agacttcaag
    13981 ttcacaggcc cagtcgccgt attgtctttt ctacaggctg cagggctagt tgatgaccac
    14041 gagaatgatt gtgagtggaa aggtcttaaa tgatgtctaa caaaaataag ggaattctga
    14101 tttttgcgat tctctataca gtcctctttg tgtttgatgg cgttaaattg ctggcttctt
    14161 taatgccatc tgccattgca aattatcttg tttatgtagt tttagctcta tatggctcct
    14221 tcttattcaa ggatagattg atccaacaat ggaaggagat tagaaagact aaaagaaaat
    14281 tcttctttgg cgtcttaaca ggatggctct ttctcattct gatgactgtt gtctttgaat
    14341 ttgtatcaga gatgttgaag cagtttgtgg ggctagtcgg acaaggtcta aatcagtcta
    14401 atattcaaag cacttttcaa gagcaaccac tactgatagc tgtttttgct tgtgtcattg
    14461 gacctctggt agaagaatta tttttccgtc aggtcttatt gcattacttg caggaacggt
    14521 tgccaggttt actaagcatt attctggtag gacttgtttt tgctctgact catatgcaca
    14581 gtttggctct atcagagtgg attggtgcag ttggttactt aggtggaggc cttgcctttt
    14641 ctattattta tgtgaaagaa aaagagaata tctactatcc cctacttgtt cacatgttaa
    14701 gcaacagcct ctccttaatc attttagcta tca
//
LOCUS       DAVBCW010000045        14692 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVBCW010000045 DAVBCW010000000
VERSION     DAVBCW010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14692
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.045"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(119..751)
                     /locus_tag="VQ514_001163"
     CDS             complement(119..751)
                     /locus_tag="VQ514_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET2499921.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAMQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKNTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAVTWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            complement(773..1645)
                     /gene="sdaAA"
                     /locus_tag="VQ514_001164"
     CDS             complement(773..1645)
                     /gene="sdaAA"
                     /locus_tag="VQ514_001164"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HET2499922.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIKNEKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            complement(1654..2325)
                     /gene="sdaAB"
                     /locus_tag="VQ514_001165"
     CDS             complement(1654..2325)
                     /gene="sdaAB"
                     /locus_tag="VQ514_001165"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HET2499923.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            2567..3139
                     /locus_tag="VQ514_001166"
     CDS             2567..3139
                     /locus_tag="VQ514_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET2499924.1"
                     /translation="MKSITKKIKATLAGVAALFAVFSPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNGDYSAE
                     NQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            complement(3191..4054)
                     /locus_tag="VQ514_001167"
     CDS             complement(3191..4054)
                     /locus_tag="VQ514_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2499925.1"
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPK
                     YENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQD
                     YLKTHHDLPIEEIRDMLEVVIYLRQHGAGELSKHAEQVVKKLWKKIEKQDTWYESDLK
                     ILNTILFSFPIEYLHLITGKILQRLEVYKNYQHLYDLRMTILLNLSTLYLYNQDKNMC
                     KQICYTLLEDAKNKKSYDRLAICYVRIGICTDDSKLIQKGFSLLELTEETSMLSHLKK
                     EVEIYYQAKER"
     gene            4520..4858
                     /locus_tag="VQ514_001168"
     CDS             4520..4858
                     /locus_tag="VQ514_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HET2499926.1"
                     /translation="MLKKYPCTMQHDQSDCAAAVVSTVLLSYKKELSIMKIREIIGTD
                     MYGTTVSGIVSGLNKLNFTVKAVRVALEDLTPKLTFPAILQVKNDLGQNHFVVLHSIK
                     EKINGTRITK"
     gene            4836..5195
                     /locus_tag="VQ514_001169"
     CDS             4836..5195
                     /locus_tag="VQ514_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499927.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAIYIPRWAITGAITGAAYAAL
                     AAAGGGGLQLVLASYGLRSALVAGIVKGLGVLGIHIGNAFANTVIRSIASAGIGAGAD
                     WILPILLMAGMGDVIIN"
     gene            5214..5426
                     /locus_tag="VQ514_001170"
     CDS             5214..5426
                     /locus_tag="VQ514_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000989850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499928.1"
                     /translation="MMVVLISVIVAFLYHKITEYVNNRKIDIVIFLSLLISHFIWNYY
                     FLPIEILIAISGANLLLSFLKKLQIK"
     gene            5841..6305
                     /locus_tag="VQ514_001171"
     CDS             5841..6305
                     /locus_tag="VQ514_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499929.1"
                     /translation="MELVLPNNYVVLEQEEMMYLDGGGVGCNWWNKRDNIAAAAVSGG
                     MSVYQTYAIKKLIANRGMMSRVVRSAITRYIGGVSMATITNALNIAATVAEVFSLGGA
                     IAYGLDIVDGKFDGYNKINLINSIIIALGMILALILVLSRVYRKYFYRDDDD"
     gene            6462..7520
                     /locus_tag="VQ514_001172"
     CDS             6462..7520
                     /locus_tag="VQ514_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499930.1"
                     /translation="MKDDQKYLLAGLYSLLVAIFYFPLIESKGIFVSILMAVLLLYLI
                     YFIATVIHIVIIKFIRKKSFKYLVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQ
                     EYCQGQPYGLLKKMLKRIRLSREIALLLATIIVYFFTHRILPLSVFTFIFSYILLFAQ
                     SYLGGNTVWIGNRRLIIDDEFEKILLSKSYIKEISSARYSEYLTCEYKNLTPIILLAI
                     FENLLDSYLLQNQSEVDLDIFYKVLPLLYKEKYTMGFNYFVSLNYLLYKVGFLGIIYD
                     NEALGDLSKQYLNRNISELQDGSFDGGIQDAVASKQIVVINEFIACLNSRCMPSQYDR
                     FFYKDRPYIFSRKSPIKG"
     gene            7835..8809
                     /locus_tag="VQ514_001173"
     CDS             7835..8809
                     /locus_tag="VQ514_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499931.1"
                     /translation="MKNKRYFFDTILMILLLISTIFCVSPVFIKLDILGTPSHAILTF
                     VLAIPLFYILSQCLHTLLLLVSSIFCKLRPIYFYFIFVIIIGARKYYRILFHQLMGFS
                     PGIAVFYKESQTTKNLFKFYYFLYFTTLISYYFFFTFVYDKPLLLPLIPFSIIIALVQ
                     KLYRIENQQLFLLKSKVLTILESKKNCEFNLQDYHEIWKLQSKSELPCVALSYISLIK
                     PYLSESVREQIDLLEVKRFKKINHPISLYGMLDVIKLNLYLRHYNEKNKYESMLKKIL
                     EVRPDFVLIEQNIDDSLNSSQPLSLSLAISEIQLLLEVYMGIKHVSTK"
     gene            9189..9563
                     /locus_tag="VQ514_001174"
     CDS             9189..9563
                     /locus_tag="VQ514_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HET2499932.1"
                     /translation="MELVLPNNYVVLEQEEMMYLDGGFSIPRWPVATAINIAFNGVLG
                     GGAISLVRNYIRNYGLRRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGD
                     AITTIWANNDVNRRDPNLNALW"
     gene            9771..10673
                     /locus_tag="VQ514_001175"
     CDS             9771..10673
                     /locus_tag="VQ514_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499933.1"
                     /translation="MIRKLIVFLLMLPLFVLWLELHVLVNNLLLNLESPLDFVISMSL
                     AFCSLILSKIVLDILYALKDLYKKEGLIKIFPFIFIRRKKVNVRFSPYFSFHRKSLSP
                     DDLRSRIIWSFILEIAIILVFILKIPFAIIMLTTIFFWTIMDINHLVFNKTEFLFNQN
                     KWQKEDSFESNLHKILMDKIQKSELSYSDLMSLLLYDAMNQSTFLTDSELFEDILKKI
                     EDSHNTLLCTGLVELLLYEISISNNNNWQEKVDKIRIQLIRINQLDFFYYTSWLRQNF
                     DFCMNREYHKMKSRKLLLSNKKIV"
     gene            10729..10968
                     /locus_tag="VQ514_001176"
     CDS             10729..10968
                     /locus_tag="VQ514_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HET2499934.1"
                     /translation="MELVLPNNYVVIDEEVMMYLDGGAYLSKRACQGICAAAGVLATA
                     AGKIAYCIGYGVLNRGCDINENPYPWDGFISATVR"
     gene            10965..11693
                     /locus_tag="VQ514_001177"
     CDS             10965..11693
                     /locus_tag="VQ514_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499935.1"
                     /translation="MSNVDKIRKIHIIVCWVYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLVINSILLMSFPILINKFFPESFLTYTVLISVFILELAIFHL
                     IRKDFDIKLTNEYKKISQFRSKVSQSPWIKYLEISSFILTIFPSILYGKVDNHVLTLI
                     FLIKICVDTTIKFLFIRLFDTSTLMKRRIFFLFALDVIAYLFLGYLLVIQKAGYLFSV
                     LLLFSNFSVPFIKEKEYELFKNSK"
     gene            12828..13067
                     /locus_tag="VQ514_001178"
     CDS             12828..13067
                     /locus_tag="VQ514_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499936.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLEIFKVNFCLTDLHHLF
                     DLYKITRDYASQTKPAIQDGVFILEDFRNILCTMM"
     gene            13617..>14692
                     /locus_tag="VQ514_001179"
     CDS             13617..>14692
                     /locus_tag="VQ514_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein"
                     /protein_id="HET2499937.1"
                     /translation="MNKKKMILTSLASVAILGAGFVTSQPTFVRAEEAPVASQSKAEK
                     DYDAAVKKYEAAKKEYEDGKAAQKKYEDDQKKTEEKAEEERKASEEEQAANLKYQQEL
                     VKYIRENDPTKKAEAKKAMDEAEKEYKKKQTEFAEVRAKVIPSAEELKKTRQKAEEAK
                     AKEAELTKKVEEAEKKVTEAKQKVDAEHAEEVAPQAKIAELEHEVQKLEKALKEIDES
                     DSEDYVKEGLRAPLQFELDVKQAKLSKLEELSDKIDELDAEIAKLEKDVEDFKNSDGE
                     QAGQYLAAAEEDLVAKKAELEKTEADLKKAVNEPEKPAEETPAPAPKPEQPAEQPKPA
                     PAPQPEKPAEEPENPAPAPQPEKSA"
BASE COUNT         4843 a         2185 c         2855 g         4809 t
ORIGIN      
        1 atctacaacc ttaaaacagt gttttgagca gcccgcagct agtttcctag tttgctcttt
       61 gattttcatt gagtattact tcattttctt ctgaaataga gttgtcgccc agtctatgct
      121 attgaaaata cgccaaaact tctaagggtt tgtgagcgat ataatcaggt tgatagttta
      181 gtagatctgc ttgctctcca aatccccaag tgacggccaa tttctgaata cctgtttctc
      241 gagctcccag catatcaaac ttggtatctc cgatgatgat ggcttgttct ggtgctagtt
      301 gatgtgtctg taaggcttgg tgaatgacat ctgccttatg gggtgcttca gggctagaac
      361 cataaatacc atcaaagaaa tgatggattt ccaagttttt ggccatgtct tgagcagtag
      421 atgtattctt tgtcgtggtg atgtagagtg gataactgct cgataactcc tcaagcaagt
      481 ctataatctg aggaaagagt tgagcttcat agatgccttt tgccttatag taagaacgat
      541 atatctgcat ggcttcagaa atttggtctt tggacaggca ggtcgcaaaa ctactttcga
      601 gaggtggtcc cataaaacca cgaatagttt tggcatcagg gctaggcacc cccagctctt
      661 taaaggtata ggtaaaggca ttgtgaatcc cgatagaact atcaacgagg gttccatcca
      721 aatcgaaaaa aatcgctgtg atagaggtca tggtttctcc tatttgataa gcttattctc
      781 cgaaaatctc tttttggagg cgacgaccag taggggtggt agcgagtcca ccttcagctg
      841 tttcacgaaa ggcagttggc atgcttgctc ctacttggta catggcatcg atcacttcat
      901 ccacagggat tttagattcg atacctgcca aggccatgtc tgctgcgatg aaagcaaagc
      961 tagctcccat ggcattacgt ttgacacagg gaacttcgac caaacctgca acagggtcac
     1021 agatgaggcc tagcatattt ttaatgacaa aggcaatagc ttgactggcc tgataaggtg
     1081 ttccacctgc agccagagtc aaggcggcag cactcatagc agaggctgaa ccaacttcag
     1141 cttgacaccc accctcagca cctgagatgg aggcattgtt tgcgatgact agtccaaagg
     1201 caccagcagc aaagaggaaa tccaattgtt gctcgtggct gaggtctaat ttttcaatag
     1261 cagcagtgag aacggaaggg agacaaccgg cacttcctgc ggtaggagta gcgcagacca
     1321 agcccatttt agcattgtgt tcattgactg cgatagcatt tcgggcagca gagagaatcg
     1381 tgtaatctga taaagttttt tcgtttttga tgtagtgatc caatttggca gcatctccac
     1441 ctgtcaggcc actacgagat ttattttcat tgaggccaag ttggacagag gctttcataa
     1501 cttccagatt gcgttccatg agaaggaaga cttcttcacg ttcgcgaccg gtcaattcaa
     1561 actctgttgt aatcatgagt tctgcgacat ttccttgaaa gtccagatct gcttgctcga
     1621 ccaattcttt gatagaataa aacatgcttc ctcctattta aagaaattga cattgtggag
     1681 atgagggatt tttcgaattt cttcgatagc ctcatcacag ttgcgactgt caacttcgat
     1741 aatcataatg gctttttcac cagctttttc acgagtgaca ttcatctggg cgatattgat
     1801 accatagcgg gaaagcgcct ctgtaacaag ggcaatcata cctggaatat cttgatgaac
     1861 gatgatgata gtcggtgtat tcatattgag agagacggca aaaccattga gttcggttac
     1921 ctgaatattt cctccaccga tagaaatacc agtcacgctg atggtcttgt gggcattttt
     1981 aacagtaatt ttagtggtgt tagggtgagg ggcattgctg tctttctgaa tggtccagac
     2041 aatcttgata ccacgcttgt gggcaatttc cagactattt ggaatttcag gatcatctgt
     2101 atccattcct aaaatacctg caacaagggc taggtctgtt ccgtgaccac gataggtctt
     2161 ggcaaatgag ttaaaaagtt ggaattcaac ttctgtcgga gtatcatcaa aaatggaaga
     2221 gacaatcttc ccaatacgaa cagcaccagc ggtatggcta ctagatgggc caatcataac
     2281 tggtccgatg atatcaaaga cagattgaaa acgaagtgat ttcatcagtt tccccttata
     2341 aaaattctta tctctattat atcaaagaat gaggggcttg gctttaattg tggatgaaaa
     2401 cctttctaat acctcaaata gcataaaaat agtatctttt atgacaaaaa acaccttatt
     2461 tagggaaata aaaaataatt ttgtaatatt tctacataaa agtgtcaaga aacagtaata
     2521 tttaaagggt atgatagaac tatagaaaga aggagaattt tcgaatatga aatcaataac
     2581 taaaaagatt aaagcaactc ttgcaggagt agctgccttg tttgcagtat tttctccatc
     2641 atttgtatct gctcaagaat catcaactta cactgttaaa gaaggtgata cactttcaga
     2701 aatcgctgaa actcacaaca caacagttga aaaattggca gaaaacaacc acattgataa
     2761 cattcatttg atttatgttg atcaagagtt ggttatcgat ggccctgtag cgcctgttgc
     2821 aacaccagcg ccagctactt atgcggcacc agccgctcaa gatgaaactg tttcagctcc
     2881 agtagtaagt gaaacagttg tttcaactgt aagcggatct gaagcagaag ccaaagaatg
     2941 gatcgctcaa aaagaatcag gtggtagcta tacagctaca aatggacgtt atatcggacg
     3001 ttaccaatta acagattcat acctgaacgg tgactactca gctgaaaacc aagaacgtgt
     3061 agcagatgcc tacgttgcag gacgttacgg ttcatggact gctgctaaaa acttctggct
     3121 taacaatggc tggtattaag aactaacgaa caaataatat gtaaagagtc gaggaaatcc
     3181 ctcggctttt ttatctttcc ttcgcttggt aatagatctc tacttctttt ttgagatgag
     3241 acagcataga agtttcctcg gttagctcca gaagggagaa ccctttttgg ataagtttag
     3301 aatcgtctgt acaaatccca atacggacat agcagatagc cagcctatcg tagcttttct
     3361 tattcttggc gtcctccagt aaagtgtagc agatttgctt acacatgttt ttatcttgat
     3421 tgtatagata gagtgtggaa aggtttaata gaattgtcat tcgcaagtca tataaatgtt
     3481 gatagttttt atagacttct aggcgttgca atatttttcc agtgatgaga tggaggtatt
     3541 caatgggaaa actgaaaaga atggtattga ggatttttag atcactctca taccatgtat
     3601 cttgtttttc aatttttttc caaagttttt tgacaacctg ttctgcgtgt tttgatagtt
     3661 cccctgcccc gtgttgacgg agataaataa caacttcaag catatctctg atttcttcta
     3721 tagggagatc gtggtgggtc ttgaggtagt cttggcattt ttgaaataaa ttgacaagat
     3781 cgctggtccc gataatggaa cgcatattga gataggtttg cataatctct gtgcgttgac
     3841 ttggttgata aagttgacag atatattcaa actcttcaaa actcatattg atttgacgga
     3901 gaagaaattc catattttca tattttgggg ttgtcttgcc actttcgatt tttgataagc
     3961 tggttcttga gagaacaccc ccacagacct cttcttgggt cagccccttt gactcacgta
     4021 tttctttata gacctttccg aaatcataac gcatgatttt ctcctttctg tgaaaaaagt
     4081 taacaaacaa taaaattttg ttaaaaatat tgtatcatag caatgtaaaa agtaaaaaag
     4141 aaagtcgaaa taaatttgtg aaaaaagtta acgtaatttt aaaatgagct taataagagg
     4201 tatgatgtag gtagaaaata aagataggag aaaaagtatg aataaaaaaa tattttgtat
     4261 tctagtatgt attatatcat tgattagtct ggccataatc tttccatggg gctggtcgat
     4321 ataatgtaag ggagcttgag ttgttaaagg aggcttagtc cttggacaaa aatctgctgt
     4381 actacaatgc ttggaaagat atcaagaaca aagaatgata cgattgaaaa taaactgatt
     4441 caagaactac tgggcagtga ttgaggtttg ggtgacatca aagaaaagtt agattaattc
     4501 taaagaattg agtatttcta tgttaaaaaa atatccttgt acgatgcaac atgatcagtc
     4561 agattgtgct gcggcagttg tttcaacagt tcttttatct tacaaaaagg aactatcaat
     4621 catgaagatt cgggaaatta ttggtacaga catgtatgga acgactgtca gtggtattgt
     4681 ttcaggtctg aataagttga attttacagt aaaagctgtt cgagtagcac tggaagattt
     4741 gactccaaaa ttaacatttc ctgcgattct tcaagttaag aatgatttag gtcaaaatca
     4801 ttttgtggta ttacatagta taaaggagaa aataaatgga actcgtatta ccaaataatt
     4861 atgttgtgat tgatgaagaa gagatgatgt accttgatgg gggggctata tatataccca
     4921 ggtgggcaat tacaggagcc attactggtg cagcatatgc agcattagca gcagcaggag
     4981 gtggaggcct tcaactagtt cttgcatctt atggattacg ctccgcactg gtagctggga
     5041 ttgttaaagg tttaggagta ttaggaattc atattggaaa tgcttttgca aatactgtta
     5101 ttagaagtat tgcatctgct ggaattggtg ctggagctga ttggatttta ccaatattat
     5161 tgatggctgg gatgggcgac gtgataatca attgagaata ggataatagt attatgatgg
     5221 ttgttttaat ttcagttatt gttgcattcc tgtaccataa aattacagag tatgtcaaca
     5281 acaggaaaat tgatattgtt atttttctat cattgctgat ttctcatttt atttggaatt
     5341 actatttttt accaattgaa atattgatag caatcagtgg agcaaattta ttattaagtt
     5401 ttttaaaaaa attacagatt aaataatatt attccatatg acatatcaac taggtcatta
     5461 gattttattg aagatataaa ttaaaaacga ggttgggaaa aaactcaatt tgaaagtgaa
     5521 attgagtaaa tttttctatt atataacgca tattatcaag atttttgaac acctgataat
     5581 atgcactttt cttattttaa agattttttg cctttcttca tttcttgaat gaattggaga
     5641 gatgacttta tttaggtgtt tgacattact atagatagga tttagcagta ctaatatgaa
     5701 ctcatattat aggtgcagac ttaaattttg gaagcaagct aaaggatatc ttatctaggt
     5761 aactaagtgc tataaacaaa gtcaacgatc acttgttagg aaacgatcct aactatgctt
     5821 aataaaaaag gagaaaataa atggaactcg tattaccaaa taattatgtt gttcttgagc
     5881 aagaagagat gatgtatctt gatgggggtg gtgttggttg taactggtgg aacaaacgtg
     5941 ataatattgc tgcagctgca gtttctggag ggatgtcagt ataccagaca tatgccatta
     6001 aaaagttaat tgctaatcgt ggtatgatga gccgagtcgt tagaagtgct ataacaagat
     6061 atattggtgg agttagtatg gcaactatta ctaatgctct taatattgcg gctactgttg
     6121 ctgaagtttt ctcattagga ggagccattg catatggttt agatatcgta gacggtaaat
     6181 ttgatggata taataaaata aatctaataa attctattat tatagctctt ggtatgatat
     6241 tggcattaat tttggtatta tcaagagtgt atagaaaata tttttataga gacgatgatg
     6301 actaaagttt ttatcaataa tttgggctcc ttgtcaactg tagtggattg aagaaaagct
     6361 aacgtctggg gaagggttta aagctttgct ttatcaaata ttgacttaca gagttgctct
     6421 attgtattga tgtattctta gtaaataata tggtgttttg aatgaaagat gatcagaaat
     6481 atttattagc gggactttat tcgcttctag ttgcaatatt ttattttcca ttgattgagt
     6541 ccaaggggat ttttgtaagt atattgatgg cggttttact tttatattta atatacttca
     6601 ttgcaacagt cattcatatt gtgataatta agtttataag aaaaaaatct tttaagtatt
     6661 tggttctata tccatttact tatgatggga gctggagatt tcaacctata aacttactct
     6721 actttccaga aatggtgaga gatgtcattc cgattaattt agttcaagaa tattgccaag
     6781 gacaacctta cggactgtta aaaaagatgc tgaaaagaat tcgattgtca cgagagatag
     6841 ctttactcct ggctaccatt atcgtctatt tttttacgca tagaattcta cctctttcag
     6901 tatttacgtt catattttct tacatattgc tatttgcaca atcttatttg ggtggtaata
     6961 ccgtttggat tggaaataga agattaataa tagatgatga atttgaaaaa atcttgcttt
     7021 caaaaagtta tataaaagaa atatcatctg cacgatactc ggaatacttg acatgtgaat
     7081 acaaaaacct tacaccaatc atattgttag ctatttttga aaatttgctt gactcctact
     7141 tgcttcagaa tcagagtgag gttgacttag atatttttta taaagtttta cctttgcttt
     7201 ataaagaaaa atatactatg ggattcaatt actttgtaag tttaaattat ttactatata
     7261 aggttggatt tttgggaatc atttacgata atgaagcatt aggagatttg tcaaaacaat
     7321 atttaaacag aaatatttcg gaattacaag atggttcttt cgatggcggt atacaagatg
     7381 ctgttgcctc taaacaaata gtagttataa atgaatttat agcttgttta aattccagat
     7441 gcatgccatc ccaatatgat agattttttt ataaagatag accttatatt ttttccagaa
     7501 agagtcctat taaggggtga aaatttgtta catttggatt aacaagtgtc ctagggatta
     7561 gaaataactc tagtatagct agatattttt gagtaactag aaatatcatt aggctaccca
     7621 tatgattgcc agaaaagatg gctgatgaat cttatttata ctgttgcagt aggcattgtc
     7681 taggataaat aagctatctt gatgggggaa attggaataa ttttagtaga aacattaaag
     7741 gattatggac aagtagcgca ggattaggtg atgcaatgtg ctaagttggc ttggtgttag
     7801 cagtagtatt atttaacaat ggaaagtaga ctaaatgaaa aataagcgtt atttttttga
     7861 cacaatttta atgattttac tgcttataag cacgatattt tgtgtcagcc cagtatttat
     7921 aaaattggac atcttaggaa ccccctctca tgccatctta acctttgtat tggcgatacc
     7981 gttattctat attttgtctc aatgcttaca tactttgcta ttactagttt cctcaatctt
     8041 ttgtaaatta agaccaattt atttttattt tatatttgtg atcattatag gagctaggaa
     8101 atactatcga attctctttc accaactaat gggatttagt ccaggaatag cagtgtttta
     8161 taaggagagt caaacaacta agaatctgtt taaattttac tacttcttgt actttacaac
     8221 actaatttca tactattttt tctttacttt tgtgtatgac aaaccgttgc ttttgcctct
     8281 tattccattt tctattatca tagcattagt acaaaaactt tatagaattg agaatcaaca
     8341 attatttctg ttaaaatcaa aggtactaac aatactggaa tctaagaaga attgtgagtt
     8401 taatcttcag gattatcatg aaatttggaa attgcaatca aaatctgagt tgccatgcgt
     8461 ggctttatct tatatcagtt tgatcaagcc atatctttca gagagcgtgc gtgaacaaat
     8521 tgatttatta gaagttaagc gatttaaaaa aataaatcat ccaatatcac tttatggtat
     8581 gttggatgtt ataaaattaa atctatattt acggcattat aatgaaaaaa ataaatatga
     8641 atcaatgtta aagaaaattt tagaggtaag accagacttt gttttaatcg agcagaatat
     8701 tgatgatagt ttaaatagct cgcaaccatt atctttatct ttagccattt cagaaatcca
     8761 acttttgctt gaagtgtata tggggatcaa acatgtttca accaagtgat aaaaccagaa
     8821 taatatttgc cattataggg gcaagttgca tattagtcag tatgtatctt attccactta
     8881 ttaaaactga gaataaattt tgggaaatac ttttatttat attaggtgta ttgctaattg
     8941 gaattgcgtt actaagtcaa aaaaaataaa ttttgaaata gtattagtga gttcgcaatc
     9001 atttattgct agtggtttag aactgttaag tttcaaacaa taattgattt taaaccttag
     9061 gtaacctata aaaacaaata aggtagagaa aggatatctt atctaggtag ctaagtgata
     9121 aaaacacagt cgtctatcac ttgttaggaa actatcctaa ctatgcttaa taaaaaagga
     9181 gaaaataaat ggaactcgta ttaccaaata attatgttgt tcttgagcaa gaagagatga
     9241 tgtatcttga tgggggattt tctattccga gatggcctgt tgcaacagcc attaatatag
     9301 cttttaatgg tgttttaggt ggaggagcaa tcagtctagt tagaaattat attcgtaatt
     9361 atggtttgcg gcgagttaca agcgcaattg ctggagcagc tgcaagatat gttggggtac
     9421 gagttgcaaa tagagtggca ggatttgcac tgtctgctat taatggattt gcagcttgga
     9481 tgtcaattgg cgatgctatt acaacaatct gggccaacaa tgatgtaaat aggagagacc
     9541 caaatttaaa cgccttgtgg taactagata tgggtattaa aatggaaaaa atttttgtta
     9601 ttattttttt tgtatgtcta tttatatcat ccataacttt tttagcctat gattttgtta
     9661 gcgaagaaat aaaaaagttg attatttgga ttaatgtcgt ttttttgatt ttaatcatag
     9721 caatgatgat ttatccaaaa ttaagaaaat gataaggaca ctgtaaaatt atgatacgta
     9781 aactaattgt tttcttacta atgctccctc tttttgtctt gtggctagag ttgcatgtcc
     9841 tagtgaataa cttactattg aatctagaat ctccccttga ttttgtgatt agtatgagtc
     9901 ttgctttctg tagtttgatt ttatcaaaaa tagttttaga catactatac gcattgaaag
     9961 atttatacaa gaaagaaggt cttattaaga tttttccctt tatttttata agaaggaaaa
    10021 aagtgaatgt gagattctcg ccctattttt cattccatcg taaaagtttg tcgcctgatg
    10081 atttaagatc taggattata tggagtttta ttcttgaaat tgctattatc ctagtattta
    10141 ttttaaaaat tccttttgca atcatcatgc ttactacaat atttttctgg actataatgg
    10201 atatcaatca tctcgttttt aataagacgg aatttctttt taatcaaaac aaatggcaaa
    10261 aagaagattc atttgaaagt aatttgcata agatattgat ggacaaaata caaaaatcag
    10321 aattaagcta ttctgactta atgtcccttc ttctctatga tgcgatgaac caatccacct
    10381 ttttaacgga tagtgaactt tttgaggata ttttgaaaaa aatcgaagat tctcataaca
    10441 cccttctgtg tacaggtctt gtcgagttac tgctgtatga gatttctatt agtaacaata
    10501 acaactggca agaaaaggtt gataaaatta gaatacagct catcagaatc aatcaattag
    10561 attttttcta ttatacaagt tggttaaggc aaaattttga tttctgtatg aatagagagt
    10621 atcataaaat gaaatctcgg aaattattac tgagtaataa aaagatagtt taagttcgtc
    10681 ctatttgtaa aacaaatgaa acaatttcaa ctaaggagga gaaaacaaat ggaacttgta
    10741 ttaccaaata attatgttgt gattgatgaa gaagtaatga tgtatcttga tgggggagct
    10801 tatttaagca agcgtgcttg tcaaggaatt tgcgcagcag caggtgtatt ggctacagca
    10861 gcagggaaaa tagcttactg tattggatat ggtgttctta atagaggttg tgatattaac
    10921 gagaaccctt atccttggga tggattcata tctgcaacag taagatgagt aatgtagata
    10981 agattcgaaa aatacacatc atcgtgtgtt gggtgtatat atttttatca ttcagggcga
    11041 ttataaatga tacggagtat tttttgctta tttttttagc ttttatctac tcaatagtat
    11101 cactacctct atattctgtg aaaaataaaa tagtatctat ctgtctagtt ataaattcga
    11161 ttctgttaat gagtttcccc attttaatca ataagttttt cccagaaagt tttttgactt
    11221 ataccgtatt aataagtgtt tttatcctag agttagcaat ctttcattta attaggaaag
    11281 attttgatat taaattgact aacgaatata agaaaattag tcagtttaga agcaaggtgt
    11341 ctcaatctcc ttggataaaa tatttagaga tttctagttt tatattaact atatttccat
    11401 ctattcttta tggtaaagtt gataatcatg tactgactct tatctttttg ataaaaattt
    11461 gtgtagatac tacgataaaa tttttattca tcagattatt tgacacaagt actttaatga
    11521 agaggagaat attttttctt ttcgcattgg atgttatagc ttacttattt ttaggatatc
    11581 ttttagtgat tcaaaaagca ggctatttgt tttcggtttt acttcttttt tctaattttt
    11641 cagttccatt tatcaaagaa aaggaatacg aattatttaa aaatagcaag taatagaaat
    11701 atggattaaa tcttagaacg gcttaaaatc agatatgata aagggaaata gtcttatcta
    11761 ggtaactaag tgctacaaac acaatcgagg atcacttgtt aggattacat tttaatcatg
    11821 cttaataaga atggagaatt cctatgggga ttgtattact ctgaagtttc gaatacctta
    11881 gttctcgtga ttttgagcta ttctagcttt agaatccttc agaacgtaaa attttaagca
    11941 gtcaaagact tagaatagcc tgaaaacatt tattttatag caattctagg cttagaattg
    12001 ctcatatata tgtatttgag ctgctcgata gttaggacag ctctattctg agaaatctga
    12061 gctatcaatg cttcagaata gtatttcgcc aatggttttt attgaagagg tgattgtgtt
    12121 atgatagtct aaaataaaat tgatttaata gagtaaatgg agaatcgtta catgaaacat
    12181 ttttttgcag gaattggtga gataaaactt gttggctact tattgtatat atgtgtagga
    12241 ctagcccctc tttttcatat atatgtcata ggttcggaac tgagttttgt gaaaatagtg
    12301 ttatctattt tgggagttat atttgtaagt atgttaacta ttgctcgaat ttatcggact
    12361 tttctataaa gaatagtttg gacttaaact gtatctaaaa acagaggttg gtcgattatc
    12421 agcctctttc ttgtcgttga aaagagtgag atatattgac ttttgaaaaa aagatgtcat
    12481 aattgagttg ggataaacct aaaagtaata tagtagattg aatctggaat agtccgttat
    12541 gactgttaaa atatttctag aaattaattt gactttccta atcgatttgt tcacatcttg
    12601 tttcaatcta ctatactttc tcgttaaatc gttcggcaat tcatggtgac atggacacat
    12661 agtagtaagc acactatgtg gcttggcaga gctaaaaact gttcccttgc gataagccta
    12721 ataagcacaa catagggagt tagagaagcc gactctaatc aaccactttg ggcaatagtg
    12781 agaactgccc cgtgcttttt tattttgaga aaatatggag tttgtcgttg aaattacttg
    12841 attgtatttt ggactatcaa gaaagattca atggaaaaac atgtcaagta tcaacgaatt
    12901 ataagtattt agagattttc aaagtgaatt tttgtttgac tgatttacat catttatttg
    12961 acttatacaa aatcacacga gattatgcta gtcaaacaaa acctgctatt caagatggtg
    13021 tttttatttt agaagatttt agaaatatcc tatgtacaat gatgtaatcg aaaggatatc
    13081 tttatgtgag tttatcggcg atatcttcta tatctaagct agcaagttgt tgcatcgtag
    13141 ctaaggattt atctaaaaat actatgaaat tggacgtcat attttttgag gataaaaata
    13201 aaagatccgc agttttaggt ttacgaagag acaaaagcgg agtatttaaa ccagttactc
    13261 tacattttac aagcgctaag aaatatgcta aagttcgtaa aacagatgtg aaagaaatga
    13321 aatggctata aagattgtcc gcaggcttaa gcttgcgttt tttgttgttt aacgaaatta
    13381 aaaaacaagc tctctcatcg gaaatgtttt tataggaatg aaggaagatg atgctatgtt
    13441 taaaaagaga atcgaaaagg gcaaaagtag tgtctttatc tttttggagt agaagcttat
    13501 gatatagaaa tttgtaataa aaatgtaata taaaacattt gacaaatatt tacgggggga
    13561 gtatacttaa tataagtata gtctaaaaat gactatcaga aaagaggtaa atttagatga
    13621 ataagaaaaa aatgatttta acaagtctag ccagcgtcgc tatcttaggg gctggttttg
    13681 ttacgtctca acctactttt gtaagagcag aagaagctcc cgtagctagt cagtctaaag
    13741 ctgagaaaga ctatgatgca gcagtgaaaa aatatgaagc tgctaagaag gaatatgagg
    13801 acggaaaagc tgctcagaaa aagtatgaag atgatcagaa gaaaactgag gagaaagcgg
    13861 aagaagaaag aaaagcttct gaagaagaac aagctgcaaa tctgaaatat caacaagagt
    13921 tggttaaata tatacgtgaa aatgatccaa caaaaaaagc tgaagctaag aaagcaatgg
    13981 atgaggctga gaaagagtat aagaaaaaac aaacggaatt tgctgaagtt cgtgcaaagg
    14041 taattcctag cgcggaagaa ttaaaaaaga ctagacaaaa agcagaagag gctaaagcaa
    14101 aagaagcaga acttactaaa aaagtagaag aagctgagaa aaaagttact gaagccaaac
    14161 aaaaagtgga tgcagaacat gctgaagaag tcgctcctca agctaaaatc gctgaattgg
    14221 aacatgaagt tcagaaacta gaaaaagctc tcaaagagat tgatgagtct gattcagaag
    14281 attatgttaa agaaggtctc cgtgctcctc ttcaatttga attggatgtt aagcaagcta
    14341 aactatcaaa acttgaagag ttgagtgata agattgatga gttagacgct gaaattgcaa
    14401 aacttgaaaa agatgtagaa gatttcaaaa actcagacgg tgagcaagct ggacaatacc
    14461 ttgctgcagc tgaagaagac ttagttgcta aaaaagctga attagaaaaa actgaagctg
    14521 accttaagaa agcagttaat gagccagaaa aaccagctga agaaactcca gctccagcac
    14581 caaaaccaga gcaaccagct gaacaaccaa aaccagcgcc ggctcctcaa ccagaaaaac
    14641 cagctgaaga gcctgagaat ccagctccag caccacaacc agagaagtca gc
//
LOCUS       DAVBCW010000046        14352 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVBCW010000046 DAVBCW010000000
VERSION     DAVBCW010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14352
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.046"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            60..716
                     /locus_tag="VQ514_001180"
     CDS             60..716
                     /locus_tag="VQ514_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HET2499938.1"
                     /translation="MPTALGYVSIGLACGTIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFGLDFALVGMFIGI
                     FASQFQIMQRRIPVRNLLIILAVVAVSFFLLLTVVSQSLAVLFATLLGCSMGVVLDGQ
                     "
     gene            706..1029
                     /locus_tag="VQ514_001181"
     CDS             706..1029
                     /locus_tag="VQ514_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HET2499939.1"
                     /translation="MVSKYLLLAVIFSDLVTWILRMIPFILVKYKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAV
                     LRLVF"
     gene            1133..1963
                     /locus_tag="VQ514_001182"
     CDS             1133..1963
                     /locus_tag="VQ514_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2499940.1"
                     /translation="MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGQAASKKEIIVAT
                     NSSPKPFNYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYQMAV
                     NNISYTKERAEKYLYAAPTVKDPNVLVVKKDDSSIKSLDDVGGKSTEVVQGTTSAKQL
                     EDYNKQHSDNPTILNYTKANLQQIMGHLSDGQFDYKISDKITVETVIKNQGLDNLKVI
                     DLPSDQQPYVYPLLAKGQDELKTFVDKRIQELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            2004..2081
                     /locus_tag="VQ514_001183"
                     /pseudo
     CDS             2004..2081
                     /locus_tag="VQ514_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC protein"
     gene            2184..3014
                     /locus_tag="VQ514_001184"
     CDS             2184..3014
                     /locus_tag="VQ514_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2499941.1"
                     /translation="MKKIVKYSSLAALALVAAGVLAACSGGAKKEGQAASKKEIIVAT
                     NGSPKPFIYEENGELTGYEIEVVRAIFKDSDKYNVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            3168..4022
                     /locus_tag="VQ514_001185"
     CDS             3168..4022
                     /locus_tag="VQ514_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HET2499942.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            4122..5495
                     /locus_tag="VQ514_001186"
     CDS             4122..5495
                     /locus_tag="VQ514_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET2499943.1"
                     /translation="MVFPSEQEQIEKFEKDHVVQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYWEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            5488..6549
                     /locus_tag="VQ514_001187"
     CDS             5488..6549
                     /locus_tag="VQ514_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HET2499944.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTFVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSGLSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSNPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGGQ"
     gene            6551..7243
                     /locus_tag="VQ514_001188"
     CDS             6551..7243
                     /locus_tag="VQ514_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2499945.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(7273..7842)
                     /locus_tag="VQ514_001189"
     CDS             complement(7273..7842)
                     /locus_tag="VQ514_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HET2499946.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADGVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            7977..8591
                     /locus_tag="VQ514_001190"
     CDS             7977..8591
                     /locus_tag="VQ514_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HET2499947.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKVLLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            8593..10239
                     /locus_tag="VQ514_001191"
     CDS             8593..10239
                     /locus_tag="VQ514_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2499948.1"
                     /translation="MKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYYRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHIGETVDR
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQKYFEHRASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            10251..11537
                     /locus_tag="VQ514_001192"
     CDS             10251..11537
                     /locus_tag="VQ514_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2499949.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISDQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVPYIKETLSSFFGQYRMNENVVSVLEVIGCD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            11598..12131
                     /locus_tag="VQ514_001193"
     CDS             11598..12131
                     /locus_tag="VQ514_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2499950.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRTAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKASAEEEETEELA"
     gene            12209..12679
                     /gene="nrdI"
                     /locus_tag="VQ514_001194"
     CDS             12209..12679
                     /gene="nrdI"
                     /locus_tag="VQ514_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HET2499951.1"
                     /translation="MKIISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(12972..14234)
                     /locus_tag="VQ514_001195"
     CDS             complement(12972..14234)
                     /locus_tag="VQ514_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HET2499952.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFSGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
BASE COUNT         4253 a         2678 c         3265 g         4156 t
ORIGIN      
        1 aagacaatat gaggagaaag atgaaagaaa aaggatttta ggagggggcg caggcagcca
       61 tgccaacggc tcttggctat gtcagtatcg gcctggcctg tgggactatc ggtgcgccct
      121 atgtgacacc tgttgagatg ggcttgatga gtctctttgt ttatgctggg agtgcccagt
      181 ttgccatgtt ggcactgatt gtggttcaag ctcctgtggc agctattgct atgacggttt
      241 ttctaatcaa cttgcgtctc tttttgttga gtttacacgc atcgacttat ttccgtcata
      301 ccagtctttg gtacaatatc ggtatgtcta gtatcttgac agatgagacc tatggcgttt
      361 tgatgggtga attggcccat acagacaagg taaatcctat gtggatgcac ggaaacaatc
      421 ttaacagcta tgtggcttgg tttgtgggga cagtagtcgg aacggctctg ggtggcctgc
      481 taccaaatcc agaaatcttt ggcttggatt ttgccctggt tgggatgttt attggtattt
      541 ttgcttcgca atttcagatt atgcaaagac ggattcctgt ccgcaatctg ctcattatcc
      601 tagcagttgt tgcggtgtcc ttctttttgc tcttgacagt ggtgtctcag tcactagctg
      661 ttctgtttgc gacgctactt ggttgtagca tgggggtggt tttagatggt cagtaagtat
      721 cttttattag cagttatttt ctctgacttg gtgacttgga ttctccgtat gattcccttc
      781 atcttagtca agtataaggg cttgcctgca atcgttgagc gttttttgaa gttcttgccc
      841 gtttccatta tctttgcctt gattctttca agcgtagtga caggtaaggt tgggagcctt
      901 cctcaaatta aatggctaga cttcttagcc gtctttccaa cagcttgggt agcctttcgc
      961 taccgcaatc tagtcggaac agttctcttt ggagtagtct tgattgccgt cttgcgtttg
     1021 gtcttttaaa tcagccataa agaaaaccta tcacagatat atatatcata taatggtgta
     1081 aatgattttt ttctgttaag attataaggt attctatttt ggaggaaatg acatgaaaaa
     1141 aatcgttaaa tactcatctc ttgctgccct agggcttgtt gctgcaggtg tgctagcagc
     1201 ttgctccggt ggtgctaaga aagaaggaca agcagctagc aagaaagaaa ttattgttgc
     1261 aactaattct tcaccaaaac cattcaacta tgaagaaaat ggcgaattga ctggttacga
     1321 gattgaagtc gttcgcgcta tctttaaaga ctctgataag tatgatgtca agtttgagaa
     1381 gacagagtgg tcaggtgtct ttgcgggtct tgacgctgac cgttaccaaa tggctgttaa
     1441 caacatcagc tacactaaag aacgtgcgga gaaatacctt tatgcagccc caactgttaa
     1501 agaccctaac gttcttgtag tcaagaaaga cgattctagc atcaaatcgc ttgatgatgt
     1561 tggtggaaaa tcaacagaag ttgttcaagg aacaacatct gctaaacagt tagaagacta
     1621 caacaaacaa cactcagata atccaactat ccttaactat actaaagcaa acttacaaca
     1681 aatcatgggc cacttgagcg atggtcagtt tgactacaag atttctgata aaatcactgt
     1741 tgaaacagtc atcaagaacc aaggtttgga caacttgaaa gtcatcgatc ttccaagcga
     1801 ccaacaacct tacgtttacc cacttcttgc taaaggtcaa gatgagttga aaacatttgt
     1861 agacaaacgt atccaagaac tctacaaaga cggaactctt gaaaaattgt ctaaacaatt
     1921 cttcggagac acttacctac cggcagaagc tgatattaaa taatttcttg aaatcatcca
     1981 ttctgagtaa gatgggtgat ttttcagttc gctaccgcaa tctagtcgga acagttctct
     2041 ttggagtggt cttgattgcc atcttgcgtt tggtctttta aattacccat aaaaaaactt
     2101 atcacagaga tagatatcat ataatggcgt aaatgctctt tttctgttaa gattataagg
     2161 tattctattt tggaggaaat gacatgaaaa aaatcgttaa atactcatct cttgcagccc
     2221 ttgctcttgt tgctgcaggt gtgcttgcgg cttgctcagg tggtgctaag aaagaaggac
     2281 aagcagctag caagaaagaa atcatcgttg caaccaatgg atcaccaaag ccatttatct
     2341 atgaagaaaa tggcgaattg actggttatg agattgaagt cgttcgcgct atctttaaag
     2401 attctgacaa atataatgtc aagtttgaaa agacagaatg gtcaggtgtc tttgctggtc
     2461 ttgatgctga ccgttacaat atggctgtca acaatcttag ctacactaaa gaacgtgcgg
     2521 agaaatacct ctatgccgca ccaattgccc aaaatcctaa tgtccttgta gtgaagaaag
     2581 atgactctag tatcaagtct ctcgatgata tcggtggaaa atcgacggaa gtcgttcaag
     2641 ccactacatc agctaagcag ttagaagcat acaacgctga acacacggac aacccaacta
     2701 tccttaacta tactaaggca gatttccaac aaatcatggt acgtttgagc gatggacaat
     2761 ttgactacaa gatttttgat aaaatcggtg ttgaaacagt gatcaagaac caaggtttgg
     2821 acaacttgaa agttatcgaa cttccaagcg accaacaacc gtacgtttac ccacttcttg
     2881 ctcagggtca agatgagttg aaatcatttg tagacaaacg cattaaagaa ctctacaaag
     2941 acggaacgct tgaaaaattg tctaaacaat tcttcggaga cacttatcta ccggcagaag
     3001 ctgatattaa ataatttctt gaaatcatcc attctgagta agatgggtgg tttctcagtt
     3061 tttagggata tagttttaaa ctatatatct gactatctaa taacaatttg tgaaatcaaa
     3121 caaattgtga gatactagta cggtattatt tttaaggaga aagaatcatg aaaatcaaaa
     3181 aatggcttgg cctagcagcc cttgctacag tcgcaggttt ggctcttgca gcttgcggaa
     3241 actcagaaaa gaaagcagac aatgcaacaa cgatcaaaat cgcaactgtt aaccgtagcg
     3301 gttctgaaga aaaacgttgg gacaaaatcc aagaattggt taaaaaagac ggaattacct
     3361 tggaatttac agagttcaca gactactcac aaccaaacaa agcaactgct gatggcgaag
     3421 tagatttgaa cgctttccaa cactataact tcttgaacaa ctggaacaaa gaaaacggaa
     3481 aagaccttgt agcgattgca gatacttaca tctctccaat ccgcctttac tcaggtttga
     3541 atggaagtgc caacaagtac actaaagtag aagacatccc agcaaacgga gaaatcgctg
     3601 taccgaatga cgctacaaac gaaagccgtg cgctttactt gcttcaatca gctggcttga
     3661 ttaaattgga tgtttctgga actgctcttg caacagttgc caacatcaaa gaaaatccaa
     3721 agaacttgaa aatcactgaa ttggacgcta gccaaacagc tcgttcattg tcatcagttg
     3781 acgctgccgt tgtaaacaat accttcgtta cagaagcaaa attggactac aagaaagcac
     3841 ttttcaaaga acaagctgat gaaaactcaa aacaatggta caacatcatt gttgcgaaaa
     3901 aagattggga aacatcacct aaggctgatg ctatcaagaa agtaatcgca gcttaccaca
     3961 cagatgacgt gaaaaaagtt atcgaagaat catcagatgg tttggatcaa ccagtttggt
     4021 aataagaaac agggaggtgg gagagaaaat tccacctctt gcttttgtat agagtataga
     4081 ttgtaaagaa gactattcgt tcatagaaag gtagagagaa tatggttttt cctagcgaac
     4141 aagaacagat tgaaaaattt gaaaaggatc atgtagtcca acattatttt gaggttttgc
     4201 gtaccttgat ttctaagaaa tcagtctttg cccagcaggt tggactcaag gaagtcgcaa
     4261 attatctggg tgagattttc aagcgtgttg gagctgaagt ggagattgat gagagctata
     4321 cagcgccctt tgtcatggca catttcaaga gttcgcgtcc agatgccaag accttgattt
     4381 tctataacca ctatgacact gtgccagcgg atggggatca ggtctggaca gaggatcctt
     4441 ttacgctttc ggtccgcaat ggcttcatgt atgggcgtgg ggttgatgac gacaagggtc
     4501 atatcacagc tcgcttgagt gctttgagaa aatatatgca gcaccatgat gatttacctg
     4561 tcaatatcag ctttatcatg gagggagcgg aggaatcggc ttcaacagac ctagataagt
     4621 attgggaaaa gcatgcagac aaactccgtg gggcggattt gttggtctgg gaacaaggga
     4681 ccaaaaatgc cttggaacag ctggaaattt ctggtggcaa taaggggatt gtgacctttg
     4741 atgccaaggt aaaaagcgct gatgtggata tccactcgag ttatggtggt gttgtggaat
     4801 cagctccttg gtatctcctc caagccttac agtctcttcg tgctgcggat ggccgtatct
     4861 tggttgaagg cttgtacgaa gaagtacaag agcccaatga acgagaaatg gccttgctag
     4921 aaacttatgg tcaacgaaac ccagaggaag ttagtcggat ttatggattg gagttgcctc
     4981 tcttacagga ggagcggatg gcctttctaa aacgtttctt tttcgagcca gcgcttaata
     5041 tcgaaggaat ccagtctggt tatcaaggtc agggtgttaa gactatttta cctgcagaag
     5101 ccagtgccaa gctagaggtt cgtctggttc cgggcctaga accgcatgat gttctggaaa
     5161 aaattcggaa acaactagac aaaaatggct ttgataaggt agaattatac tataccttgg
     5221 gagagatgag ctatcgaagc gatatgagcg caccagccat tctcaatgtg atcgagttgg
     5281 ccaagaaatt ctatccacag ggcgtttcag tcttgccgac gacagcgggg acaggaccta
     5341 tgcatacggt ctttgatgcc ctagaggtac caatggttgc atttggtcta ggaaatgcca
     5401 atagccgaga ccacggtgga gatgaaaatg tgcgaatcgc tgattattac acccatatcg
     5461 aattagtaga ggagctgatt agaagctatg agtagagata ttatcaagtt agatcagatc
     5521 gatgtgactt ttcaccaaaa gaagagaacc atcacagcgg ttaaggatgt gaccattcac
     5581 atccaagaag gggatatcta cggaatcgtt ggatattctg gagcagggaa atcaaccttt
     5641 gtacgggtga ttaacctctt gcaaaaacca tctgcaggga aaattaccat tgacgacgat
     5701 gtgatttttg atggtaaggt caccttgacg gcagagcagt tgcgtcgtaa acgtcaagat
     5761 atcgggatga ttttccagca ttttaacctg atgagccaaa agacagcaga ggagaatgta
     5821 gcctttgccc ttaaacactc tggactcagc aaggaagaaa agaaggctaa agtagctaag
     5881 ttgttggact tggttggttt ggcagatcgt gctgaaaact acccttcaca actatctgga
     5941 gggcaaaaac agcgtgtggc aattgcgcgt gccttggcca atgatccaaa aatcttgatt
     6001 tcagacgagt caacttctgc ccttgatccg aagacaacca agcagatttt ggccttgttg
     6061 caagatttga accaaaaatt aggcttgact gttgtcttga ttacgcatga aatgcagatt
     6121 gtcaaagaca ttgccaaccg tgttgcagtt atgcaggatg ggcatttgat tgaagagggt
     6181 agtgtgcttg aaatcttctc aaatcctaaa caacctttga ctcaagactt tatctcaaca
     6241 gctacaggta ttgacgaagc catggtcaaa atcgagaagc aagaaatcgt ggaacacttg
     6301 tctgaaaaca gtctcttggt gcaactcaag tacgctggag cttcaacaga cgagccactt
     6361 ttgaatgaat tgtacaagca ttaccaagta atggctaata ttctctatgg gaatatcgaa
     6421 attctcgatg gtactcctgt tggagaattg gtggtggtct tgtcaggtga aaaagcagcg
     6481 ttggcaggtg cccaagaagc cattcgtcaa gcaggtgtac aactaaaagt attgaaggga
     6541 ggacagtaag atggaatcat tgattcaaac ctatttacca aatgtctata aaatgggttg
     6601 ggctggtcag gcaggctggg gaacggctat ctacttaact ctttatatga cagttctttc
     6661 cttcattatc ggaggcttct tggggctagt ggcaggtctc tttctcgtct tgacagcgcc
     6721 aggtggtgtc ttggagaata aagtcgtatt ctggatttta gacaaaatta cctcaatttt
     6781 tcgtgcagtt ccctttatca tcctcttggc aatcttgtca ccactttctc acttgattgt
     6841 taaaacaagt atcgggccaa atgcagccct tgtcccactt tcttttgcag tctttgcctt
     6901 ctttgcccgt caggtgcagg ttgtcttggc tgaattggat ggcggtgtca ttgaggcggc
     6961 tcaagcgagc ggagcgactt tctgggacat cgtgggtgtt tacctatcag aaggtcttcc
     7021 agatttgatc cgtgtgacga ctgtgacctt gatttccctt gttggggaaa cagctatggc
     7081 cggtgcggtt ggagctggtg gtatcggtaa cgtagccatc gcttatggat ttaaccgcta
     7141 caatcacgat gtgaccatct tggcaaccat cattatcatt ttgattatct ttgcaatcca
     7201 attcttggga gatttcttga ctaagaaatt gagccataaa taaaaaagag ccgcgtggct
     7261 ctttttaact gatcagattt tctgtgcaaa ttttttactc aaggcttgtc caatcaaggc
     7321 acccactagg gctccgatga caatacttgc gataaataga aggacagttc cagggtttgg
     7381 agcgaccatg atgcggtcga tatattcttg ggattttcct cttgccagaa gagtagccat
     7441 ataggctttg ggcgcaatcc acataagcaa gattggtcct gttgtactaa aggcgaaaat
     7501 aatgaaagaa aggaagttct ttgttttgtc cttgtatttt cctaaatgag ctactccatc
     7561 tgctaggagg ccacagataa ttccaggaag gaaggaacca gcaccgtgtt tagttcccaa
     7621 gaaaaagagg gcaatgacaa ggccgatagt ggtaatggct ccaaagcgcg gaacttttgc
     7681 gactaggatc atatagacgc taccgccgac aagggcagta aaggcaggcg cataaaacat
     7741 gtttccagtt tggtcaaaga gattgcccaa aaggacacca atccccatgc agaggaagta
     7801 aagaactgca aaaagcagtg tagttaagat agatttcttc atgaattgtc tcctgataat
     7861 tttttcacaa ttctcatttt accatggttt ggtgggattg taaatggata ctagaagttt
     7921 ttgaaaacgc ttgaaatatg atataatggt ttcatagtta tttttagggg aatatcatgg
     7981 gtagattttt agactttgtc tttaatcgtt tctttttagg gatgattgcg acagccttct
     8041 tttggctatt aactttagca ggagggatta tccttggtct agcgccggct agtgccacct
     8101 tgatgagctt atatgcagaa catggttata gctttcggga atacagtttg aaggaggctt
     8161 ggtctcttta caagcaaaat tttgtctcaa gcaacctgat tttctatagc tttttaggtg
     8221 tgggtctagt tttgacctat ggtttgtatc tcttggtgca attgcctcat cagactattg
     8281 ttcatttgat tgcgaccctt ttgaatgtcc tagtagttgc cctgatcttt ttggcttata
     8341 cagtatcttt aaaattacaa gtttattttg ccttgtccta tcgaaatagt ctcaaattat
     8401 ccttgattgg catctttatg agtctagcag ctgtggccaa ggttctcctt gggactgtgc
     8461 tacttgtagc aattggttac tatatgcctg ccctgctatt ttttgtagga attgggatgt
     8521 ggcatttctt tatcagtgat atgttggaac ctgtctatga aatcatccat gaaaaattgg
     8581 cgacaaaata gaatgaagca gttttggcta catacgcttc taagaaccta tagttcagtg
     8641 atgatcatta tcattgcgag ttttgcaatc ttactctctt acgctgactg ggattcacgt
     8701 gaaaaggaag cccagagagt agcccagcgt gtaactgcta gaacagtgag tgaaattgaa
     8761 tattactata gagagtcaac ccagatagct caggctttag ttgaaaatca agctcgtatt
     8821 gagggaatct ataaatactt tagccttagc atgccagact atttttactg gcaattagag
     8881 cggaaagctt cgccttatat atcagtctct ctgtatgaaa atgttgatga cctctatgtt
     8941 cgaaatgatt ttgtaactgg ggtggccatt gcttttcaag attacaagga agtctatgtt
     9001 tctactaaag acaaacgtag tggagaaaaa atcagggctg aggatttcaa accagcagga
     9061 aatagttttg ccattccggt gtcagatcca gtgtcagatc aagatttagg agtgatttac
     9121 atctccttgg atcctgctgt tttataccat gccattgata atactagagg tcatactccg
     9181 atggcagtaa cagtgacctc accttttgat acggagattt ttcatattgg tgagacagtt
     9241 gatagggaga gtgaaaattg gctagttggc ttaacttctc atggatatca ggttcaggtg
     9301 gcagttccta aaaactttgt tttacaagga acagtgacta gctctgcttt gattgtgggt
     9361 ttgagccttc tctttattgt cattctttat ctgactttga ggcagacttt tgctaattac
     9421 caaaagcagg tagtggattt agtggaatcc attcaagtca ttgctcaagg cgaagagggg
     9481 cgtcggattg acatttccga aaaagatcag gaattactcc taatcgcgga gacgaccaat
     9541 gatatgttgg atcgattgga aaagaatatc catgatattt accagttaga gcttagtcaa
     9601 aaagatgcca atatgcgagc cttgcaggcg caaatcaatc ctcattttat gtataatacg
     9661 ctggagttct tgcgcatgta tgcagttatg cagagtcaag atgagttggc agatatcatt
     9721 tatgaattca gtagtctctt gcgtaacaat atttccgacg aaagagagac cctcctcaaa
     9781 caggaattag aattttgccg taaatacagc tatctctgca tggttcgcta tcccaagtcc
     9841 attgcctatg gtttcaagat agatccagag ttagagaata tgaagattcc caagtttacc
     9901 ttgcaaccgc tggtagaaaa ctatttcgcg catggtgttg accacaggcg gacagataat
     9961 gtgattagca tcaaggctct taaacaggat ggttttgtgg aaattttggt ggtcgataat
    10021 ggtagaggaa tgtcggctga aaagttggca aatatccgag aaaaattaag tcagaaatat
    10081 tttgaacacc gagccagcta cagtgatcaa aggcagtcta tcgggattgt caatgtacac
    10141 gagcgttttg tgctctattt tggagaccgc tatgccatta cgatagagtc tgcagagcaa
    10201 gccggtgttc agtatcgtat tacaattcaa gatgagtaga aagggagaaa atgtataaag
    10261 tattattagt agatgatgag tacatggtga cagaaggtct gaagcgtttg attccctttg
    10321 ataagtggga tatggaggtc gtcgcaacag ccagtcatgc cgatgaagct ctagaatatg
    10381 ttcaggaaaa tcctgtcgat gtcatcattt ccgatgtcaa tatgccagac aaaacagggc
    10441 ttgatatgat tcgggagatg aaagagatct taccagatgc tgcctatatc ctgctctcag
    10501 gttatcagga gtttgattat gtaaaaagag caatgaacct tagtgtggtg gactatttgg
    10561 tcaagcctgt tgataaggta gagctgggaa atctgctgga gaagattgca ggtcagctcg
    10621 gcgagagagg gaagaaaagt cagactctta gtcaagaatt agacgaggct ggatttgtta
    10681 gttatttagg ggataaggag aattggtgga taggtctatc caaggaaaaa caaggttcct
    10741 tcaccattcc ctactatgtc ttgggccaag actggcagat tttcatttct gaccaacccc
    10801 tagatggttt agtcgttaca ccttttgaag ctccttatca agaacacttt gaacgctgga
    10861 agctgaatgc tgagaaaacc ctcttttacg gttctgtaaa tttgcagcag tctgagagtc
    10921 tctttgccta ttacgaaccg atttataggg ttatcattca gggaaatctc aatcaaatcg
    10981 tagaagagtt aaatctcttg gagaaggtag ttcttgaaaa tacgccgcgt gttccgatta
    11041 ctaaacagct ttttattcag tttgtcatgg atgttttcca tttatttgaa catctcaaag
    11101 ctgatgacct gacagacatc gtcaaaacca ttcatgctat tcaatccttc gatgaattgg
    11161 ttccttatat caaggaaact ctgagcagct ttttcggtca ataccgtatg aatgaaaatg
    11221 tggtcagtgt gctggaagtc attggttgtg attaccaaaa agagctttcc ctcaaggata
    11281 tcagtaaggc tctctttatc aatcctgtct atctagggca gttgattaag cgtgaaaccg
    11341 attcgacttt tgcagagtta ctaaacaaac aacgtattaa ggctgcccaa caacttttgc
    11401 tttcaactag tgacagcatc gaagatattt gttatgctgt tggttacagt aaccttggat
    11461 atttctataa agttttccga aaattgtgcg gaaaatcgcc aaaagcctac cgaaaacagg
    11521 tagaaactat actataagat ttgtattcct ttacaaaatg tgctataata taaatattat
    11581 tattaggagg ttctatcatg aaaaagaaac caatttatct atgggtcttg ctaatcttgt
    11641 ctgcccttat ttcagttccg tctctgtttg gaattgtgag tcccttgcct agtaaagaag
    11701 cccttcgtac tgctcagaaa caagttgcag gggtcaatgc tcagcaatta gaagaccagc
    11761 ttaattatac ctatagagta gcagaagcat ctcattctat ttttaatgtt gctttgattg
    11821 tgctatctac tattttagtg gtcgtagcga ttgtcttcct tgttcgtaaa aatttgcaat
    11881 atgcaaacta tacttacgtt ggctatgttt tgttagctat tattggttcg atttatggct
    11941 atgttggttt acaagacgct gtgcagttgg ttcaagatga gagcatgcgt ttgacagtga
    12001 gtattggttc aaaagctgtt agcattttct atatcgtcat caatgttctc ttcctagccc
    12061 ttgtcttcta taagatgtgg cgacaacaga aagcctcggc agaagaagag gaaacagaag
    12121 agcttgccta agtattgaca aaaaagaact atcaagatac aatcttggta gttgtttttt
    12181 gattatagat aaaatcatgg aggtacatat gaagataatt tctttagttt atattagttt
    12241 gagtggcaac actgaaagtt ttgtgacgcg cttgaaagac tatctcttgt cccagtacaa
    12301 agggattgag gttcaaaaga ttcatatcaa ggatttagtc aaggaaggca aaaatttcta
    12361 tgaaatggac catccctatg tcgctttttt gccgacctat ctcgaaggtg ggaatggcgt
    12421 ggataacgga gatgttgaga ttttgacgac accggtggga gattttattg cctatggtaa
    12481 caatgctagt aagtgttttg gtgtggttgg gtcaggaaat cgtaacttta ataaccaata
    12541 ctgcctaaca gccaagcaat acagtcaacg ctttggcttt cctgtattgg ctgactttga
    12601 aatgcgaggc atgctggaag atatcaaaca tgtcgcagct attatcgcag atttgtatga
    12661 gttggaaaag gagaattaat actcaatgaa aatcaaagag caaactagga agctagtcgc
    12721 aggttgctca aagcacagct ttgaggttgc agatagagct gacgtggttt gaagagattc
    12781 tcgaatagta taaaattctt ataagaggtg gttgaagttg ttcaactgtt ttttgagtat
    12841 aaacagtctt taagaacgta aaaaagcata agttcaggtc ttcaaaacct tgttcttatg
    12901 ctttttatca tagaaatatt tgctgaattt tctcttgaaa tgaaacctta tcttcatctc
    12961 gcgcttgtcc cttaccctgc ttttccgatt gcgagtgcgt agataaagaa gatggcgaag
    13021 ccaaagagga aaatcaatcc cgcaatggca aggtgtttga gccaagaagg aagatttttg
    13081 ttttcacgcg ttaccaaggc gtaattcaaa agagcaaaga aaggtgttgt caggaaagag
    13141 ccaatcatgg caaagcggag catggttgaa acctgaccag agaagaactt gataatgacg
    13201 ataccgatga tagcagtgat ggtcatccag atgttcaaag atttacgatt gtcctctttt
    13261 tgactgatta gcagtcggag agattcctga ttaacgcgag aatagccatc gataacagtt
    13321 ataactgttc caaagataca gaggaaggca ataaaggtaa tcaagtaacg ggaccattcg
    13381 ccaagaacag aggcatacat gcccacgaat tgagagatgt atttggctga agcagcttca
    13441 accgcctgcc ctgtaggata ctgaatcagt gctcccagtg ccacaaagaa gacggctagg
    13501 atagctgttc caatataacc agtgttaaag tcaaacagag cgtcttctgt gttaaagttg
    13561 acggtctttc tcttttcagc tgaccaaagt gaattgatgg ctgaaatttc aataggagcc
    13621 ggcatccatc ctaggaggga gacgatgaag ggcagagctg ccatttgcca aggtgtcttc
    13681 tcgacaaaat cagaactgta ttctggatgc ttgaccgccg caatgataac tgccagaaca
    13741 gtcgcaatgg ttaaggcaga cataatccat ttgaccatgc cgtctaaaag tttgtagcct
    13801 ccaaagagta gcatagccca aatgattgca acgagaatga gggaccactg agtaatgcta
    13861 agtccaatca ttgggaaggc actggcgatg atagctgagc acagaatggc aacaccagcc
    13921 gtgttgacca tagccgaaaa gacattgagg ataaagaaaa tccagagata gagttttcct
    13981 ttttcggcat aaccttcaac caaagtcttt ccagtatcag ctgtgtattc agcaccaaaa
    14041 cggaaaaatg gatatttaaa gacattggct aagatgacca agagaagtag agaccaaccg
    14101 taagaaccgc cagcttgagt tgaggataca atgtgggaac ctccaacagc ggcagttgcc
    14161 attaggattc caggtcccat tgcttttagc ttgcttgccc aggttgattg atttaaggaa
    14221 atagcttgcg acatgataaa tactcctttt tgaattttca gaataatctg tatatgtaat
    14281 ctatattcta caaaaaattt tggaaaaatg caagaatatt ttgaaaaaag atagaaaatt
    14341 tacaaagaaa at
//
LOCUS       DAVBCW010000047        14267 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVBCW010000047 DAVBCW010000000
VERSION     DAVBCW010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14267
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.047"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(108..941)
                     /locus_tag="VQ514_001196"
     CDS             complement(108..941)
                     /locus_tag="VQ514_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET2499953.1"
                     /translation="MNHKIAILSDIHGNATALEAVIADAKTQGASEYWLMGDIFLPGS
                     GANDLVALLKELPITASVRGNWNDCVLEALDGQYGLEDPQEIQLMRMTQFLMERMDPA
                     TTVWLRSLPLLEKKEIAGLRFSISHNLPNKNYGGDLLVENDTEKFDQLLDAETDVAVY
                     GHVHKQLLRYGSQGQQIINPGSIGMPYFNWEALKNHRAQYAVIEVEDGELVNIQFRKV
                     AYDYEAELELAKSKGLPFIEMYEELRRDDNYQGHNLELLASLIEKHGYVEDVKNFFDF
                     L"
     gene            complement(931..2778)
                     /gene="uvrC"
                     /locus_tag="VQ514_001197"
     CDS             complement(931..2778)
                     /gene="uvrC"
                     /locus_tag="VQ514_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HET2499954.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGIHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDEAYFKSMAQEVSDFLKGQDDKIIDDLKSKMAVAAQSMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDTKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGRPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMRHFKSLTKIKEA
                     SVDEIVEVGVPRAVAEAVQRKLNPQETEILLQVAEERVDYQTEGNHNKP"
     gene            complement(2818..3519)
                     /locus_tag="VQ514_001198"
     CDS             complement(2818..3519)
                     /locus_tag="VQ514_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HET2499955.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKMGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            complement(3598..4830)
                     /gene="murN"
                     /locus_tag="VQ514_001199"
     CDS             complement(3598..4830)
                     /gene="murN"
                     /locus_tag="VQ514_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HET2499956.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSEYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            complement(4835..6055)
                     /gene="murM"
                     /locus_tag="VQ514_001200"
     CDS             complement(4835..6055)
                     /gene="murM"
                     /locus_tag="VQ514_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HET2499957.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            complement(6167..6946)
                     /locus_tag="VQ514_001201"
     CDS             complement(6167..6946)
                     /locus_tag="VQ514_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HET2499958.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALATNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIRDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            complement(7015..8676)
                     /locus_tag="VQ514_001202"
     CDS             complement(7015..8676)
                     /locus_tag="VQ514_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET2499959.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPEHIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKILSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEDYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            complement(8851..11073)
                     /gene="recJ"
                     /locus_tag="VQ514_001203"
     CDS             complement(8851..11073)
                     /gene="recJ"
                     /locus_tag="VQ514_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HET2499960.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKTYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            11350..12108
                     /locus_tag="VQ514_001204"
                     /pseudo
     CDS             11350..12108
                     /locus_tag="VQ514_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            12148..12912
                     /locus_tag="VQ514_001205"
     CDS             12148..12912
                     /locus_tag="VQ514_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET2499961.1"
                     /translation="MISLFGLAAAKPVQADTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITELGKKKGLKFK
                     FVELGSYPELITSLHAHRIDAFSVDRSILSGYTSKRTELLDDSFKPSDYGIVTKKSNT
                     ELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            12925..13602
                     /locus_tag="VQ514_001206"
     CDS             12925..13602
                     /locus_tag="VQ514_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2499962.1"
                     /translation="MIDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILAHIFVEFYQNTPLLVQFVIVFYGLPLISDHTIMIPIYWTAVLCMGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLIILPQTFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            13612..>14267
                     /locus_tag="VQ514_001207"
     CDS             13612..>14267
                     /locus_tag="VQ514_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2499963.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPVLRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNTVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLCILIALIYFSFNLAISSLSHMLAKRWQQAA
                     "
BASE COUNT         4034 a         3402 c         2542 g         4289 t
ORIGIN      
        1 ctagcttcct agtttgctct ttgattttca ttgagtataa gatatactaa aagcgattgg
       61 accagccaat cgctttttta gtttgcattg gctattttag gaaactctta caaaaaatca
      121 aaaaaattct tcacatcctc tacataccca tgcttttcta ttaagctggc taaaagttcc
      181 agattgtgcc cctgatagtt atcgtcacga cgcagttctt catacatttc aataaaggga
      241 agccccttgg acttggccaa ttctaactca gcctcgtaat cataagcaac tttacgaaat
      301 tggatattga ccaattcccc atcttcaact tctatcacgg catactgggc acggtgattt
      361 tttaacgcct cccaattaaa atagggcatg ccaatcgacc ctggattgat gatttgttgc
      421 ccttgacttc cataacgaag caactgcttg tgaacatgac cataaactgc cacgtccgtt
      481 tccgcatcta gcagttggtc aaatttctct gtatcattct caactagcaa gtcaccacca
      541 tagttcttat ttggcaaatt atgagagata gaaaagcgca gtccagcaat ttctttcttt
      601 tccagcaaag gcaagcttcg tagccagaca gtcgttgcag gatccattcg ctccatcaaa
      661 aactgagtca ttcgcatgag ctggatttcc tgtgggtctt ctaagccata ttgcccatct
      721 aaagcctcaa ggacacaatc attccaattg cctcgaacac ttgccgtgat aggaagttcc
      781 tttaacagag cgaccaagtc atttgcacct gaaccaggga ggaaaatgtc tcccatgagc
      841 caatattcac tggctccttg agttttagca tctgcaatca ctgcttctag cgccgtcgca
      901 ttgccatgaa tatctgataa aattgcgatt ttatggttta ttgtggtttc cttccgtttg
      961 gtaatctact ctttcttccg ccacttgaag caagatttct gtttcctgcg ggttcaactt
     1021 tctttgcaca gcctctgcta ctgctcttgg tactccaact tcgacaatct catccacact
     1081 ggcttccttg attttggtca gagacttgaa gtgcctcatg agattttgct tgcgtttagg
     1141 tcccagacca tcaatcccat ccaattgaga tgagaaagaa tttttggagc gcagttggcg
     1201 gtggaaggta atggcaaagc ggtggacctc atcttgaatg cgttggagga ggaaaaattc
     1261 ctgagaattg cgagacaagt ccaccacttc cagcggatct ccaaagagca attcatgggt
     1321 ttggtgctta tcattctttt gcagacctgc aattggaata tccaagccca gttcttcttg
     1381 gatgacttgc ttagcaatat tgacttgacc ttgcccccca tcaatcacaa tcaaatctgg
     1441 aggagtcaaa gcctcacgct gtactcgacc atagcgtctt cgaatgactt ctctcatact
     1501 agcatagtcg tccggcccaa caacggtttt tatcttgtac ttacggtaat cttttttact
     1561 tggcctgccg ttaacaaaga ccaccatggc tgaaacagga ctggtcccca tgatgttgga
     1621 gttatcgaag gactcgatgc gaactggagt cggaatttgg agcaaacgtc ctagattttc
     1681 aatagctcct tgggtctttt cgactgattt ttctagcagg ttaaatttct gctccagact
     1741 aacacgtgca ttttttatgg ctagattgac cagctgtttt ttctctccac gttggggttt
     1801 gagaatcttg gtatccacca aagccttgac agcttcttcg tcaatatcct gcggaatcag
     1861 tacctcattg ggaactaggt gagatttttc ttgatagaat tgtcccacat aggtcaagaa
     1921 atcctcatct ggatcattga agtaggggaa gagattgaca tcgcgctcga tgagcttgcc
     1981 ctgacgaaca aagaaaacct gcacacacat ccagccctta tccacatagt agccaaagac
     2041 atcgcgattt tggagatcct tagccatgac ccgttgcttg gttcgaagcg ttccaatagc
     2101 ctgaatcagg tcacggtatt ccgccgcacg ttcaaactcc atactttgtg ctgctactgc
     2161 cattttactc ttgagatcat cgatgatttt gtcatcctga cctttcagaa aatcagacac
     2221 ctcctgagcc atagacttga aataagcctc atccttctta cagatggtgt gggccataca
     2281 ctggccgata tggtaataaa aacagacctt agagggcggg ttggtacatt ttcgaaaagg
     2341 gaaaatccta tctagaagtc gcttgatttc attggctgcc cccacatctg gataagggcc
     2401 aaaataaaga ccaccgtcct ttttgacctg acgagtaatg ataagccgtg gatagcgctc
     2461 attggtgatt ttgatgaaag gataggactt gtcatccttg agcatgatat tgtacttggg
     2521 cttattttcc ttgatcaagt tgatttccag cagaagcgcc tcaatattgg actcagtgac
     2581 gataaattca aaatccacaa tttcagacac cagagcctct gtcttggtat catgaattcc
     2641 acgaaaatag gaccgtactc gattacgcag atttttagcc tttcctacat agataatggt
     2701 gccattttta tccttatgaa tgtagcaacc agggctggtc ggcaagagct ctagttttga
     2761 tttaatcaag ttgttcatag ttccattata gcaaaaaaag tagggaaaga tagtacccta
     2821 ctcactagtc tcatatattt ttcggaggtt tgcaacatcc tcttcctgga taccataaaa
     2881 ggaacctgct ggttgcatga cagacttctc atctgtatgg tccaagccaa tcgcatgacc
     2941 taactcatgt tctgccgtat ggacaaggcg ttcataggag tagccatagt atggattgga
     3001 caaataataa tgattcaacc gcaccgttac ggacaagaat tgccctgtta agagattagt
     3061 ttgactttcc gcctctcctg ccacaggagt gcctccgtcg ttcatctccg tagccgtaat
     3121 atccgcctta ctagactcag tcacgagttc aaagttaaaa gcaccagttt gattccagtt
     3181 ctggattgct tctaaataag cctcttgaaa gcgtgaatcc atctgcggat ccaggtaaat
     3241 acgagcagag gcctgtgccc accttccttc agaatgctgg tgactatctg tctggcttaa
     3301 cttaggcagt tgccctgttt tctcccattg gtcaatactt tgttgaccca tttttactga
     3361 ccgttctgct tgctttagcg ctccttgaaa atccccgttc agataccaga gaagtccgaa
     3421 agcaagcaca cataagagca caactatcca aaccagacgc caaaacaaac gccacacaaa
     3481 agaaaagaaa gcccctatca aacgaaaaag ccagcgcatg tctctccacc tttctagcaa
     3541 ataagaatta ttttactaaa agaagctaaa agaaatctaa atactgactt tttcatctta
     3601 acgtcctact atttttttga gtaactggat agctttgtat tttaaaggcg atggatggta
     3661 acggaaagta cctgctttgc gtacaatata gccattaaaa ttctgtttaa aacgcaaaac
     3721 accatcactt ccatcaaaaa tcccttgaat gcctaggaag ttgtatttag gtattccacg
     3781 ttttatgctt tccaacataa catatttttg aagcagtgca ggggcataga acttattaaa
     3841 ctcagtgtag gaaccactaa agagataagt cgtttcctga ggcatataaa caaataaact
     3901 cccagctaaa acaatatctt cttctccata tttttcaatc aagtctcgcg cttctgcttt
     3961 tcgaacttca aacgtttcaa attgactaga atattctctc agttgatttt gttttttctc
     4021 agaatgagga tttttactca aatcaagtcg caacttgtcc aagttttctt ctagtttact
     4081 ttgttcacct tgcaatttgc tcatatactc cgaaaaattc aagcttgcta tgagaaactc
     4141 cgcttgttct ccaaaagtat cataaaaatg ctcataatat tctaaacttt tatcactata
     4201 ttctctacgt tcagaggttt cttttgttat attcttaaaa atcgatagtt cttcacgttt
     4261 taactttttc aaccgaatgc caaaggtttc agcctttttc accaagggtt tacccttttt
     4321 gctaaaactt ttaagcaaac tcttttcagt taattcagtt aaatctttat agtataacca
     4381 atctggttct ccacctgggt aacctgttgt taagccatca aattgataac ctaaatcagt
     4441 caaaccttga ataatacttt ttttctcagc atctattgga ttaccttggc tatcaaaagt
     4501 ttgataagtt tcataaggtt ttacaagcaa ctctaataca ccattttgct tggcatattc
     4561 ttttaactct gcataaaaaa ctggaagagc atcttgttgg gtataaatcg gccccgaatt
     4621 gagttccata tgcagaccac ccagcatggg caagctataa accagagctg caacttgaat
     4681 ttctccttct tgtttcaaag caagataaac aattcgagcc cctctttttt ctagcaaatc
     4741 ccccatctgg acagattgca taaaggaacg agaagaaacc tgatcagaat aagtctgaaa
     4801 ctcttctttc gtgagtgttg ttagtgccat atacttactt tctatgtttt tttcttaatg
     4861 ttttacggaa atcaagagca agtcttaaca gaggatagag aggatgagtg ggcattgtaa
     4921 attcacccaa gtattcttca atcgttggat taaatttttc cttaaaatga taaagtccac
     4981 cattgagaga gttttcaaca ccacctaaat tttgccagac catacctcgc tcaaaggcat
     5041 agcgagccgt ttcataccat gttaaaattg gtgcattgta acgtttaaaa tcatcatcca
     5101 taccagcata tatattgaca gaggtagtac caaattccaa actcaaagta gccgctaaag
     5161 gaactctcgc ttgacctaca tctatatatt cctgcaagaa ggtcaattcc tctaacaaac
     5221 gttctttttc cttcttctgc gcttctactt ttgaagttcg agtcgactca gtaaaggtct
     5281 cttccaaggc tctatttttc gctaactgtt cttctaactc ttgcgaacgt ttagaaacat
     5341 ccaaggtggc caaggtgata taggccttgt ccttaaaatt atctaacaat tttttataat
     5401 aggcttcatt cctcaaatga atctcttttc gcttctcagt ttttttcatc aactccgaaa
     5461 atgaatctaa tagttccagt ccaccatatt gaatctcaag ccctttgttt cgtgctgttc
     5521 gaatagcctg ttttgttgac ttggaaagtt tatcttcttc aaaattttcc ttgtatattt
     5581 tcgcctgaat acgaggttga atggtgtctc ccatttcctc cgtttttcct gaccacctta
     5641 ctcccatttg ttgcaaacta tcaataatag ccagattttc aggaaattct gtcttttcct
     5701 gattgattaa actttgagat aggcaaatac ttgggtcaaa agtcacaaaa accgctctct
     5761 tactgcgagc ataggactta atagactgaa tggcaaaatt caagagttct ttatccccat
     5821 aatccaatat aggtcctctt gggatgtaaa acattttata gcctagcgga agagttctaa
     5881 tcaaaatact agctgtcgcc agtaattttt cttccctgta aacaccaaac ttctcatgtt
     5941 gccaattaga cttaacttcc tcccaagcac tactttgtaa tacattggct aattcatgtt
     6001 ctttgacaaa ctgatcatat tctaatgtgg gaatgccaat ttgataacgg tacatttcct
     6061 actctctttc ctccagtatt ttattactat tctactactt tcgtttgaaa agtctagtga
     6121 aaacatattt tattttagtt ctactccccc tatgctgaag ttcaaactaa gtcagtctct
     6181 cttctaattt gaaatcaatt ggtagggttg ttaaaaaaac ttccaattgt ttttcccagt
     6241 agtaccactc gtgagttcca gcgctatggc tataggtcac atcaaaacct agttttttga
     6301 gatttttcac tgcgagatta ttggcttcgt acaagaaatc ctgttcgcca caccacgccc
     6361 aaagtttggt ctttttatcc gattttttag ccagactttc aagagaatag ggactagttg
     6421 tccagtctct aatctctcca aaaacacctc tccagtaggc tggacttccc agattttgac
     6481 tttcaggaga aaagttttga aagctgaggg cacctgaaaa actagctgca tgagaaaaac
     6541 gatttgtcgc aagagccagt ttgaagcagc cgtaacctcc catagaaaga ccagcgataa
     6601 aggtcttttc acgcttgcta gtcatattag ggaagaagcg tttcagaacc tgtggcaatt
     6661 cctctgctag agccgtgtag tagtcaaaac catactgggt atcggtgtac caaccattgc
     6721 tggtattggg cataacaacg atgagattag ttcctcgaag caagcgttct acattggtcc
     6781 gcttaagcca actattatga tttccagaca tcccgtgcaa aaggtacaag acgggaatat
     6841 cttcacactc tggttcttcc actcgattgg catcagggta gaggacattc accccccact
     6901 ccatatccaa tacttgtgag taatactcga ttttcattac tgccatgatt ttctccttct
     6961 atttttctat aagaaaaagc cgaaactcga ctttctctct gtttatttca acgattattt
     7021 tgcttccatg actactggta aaatagctgg acgacgcttg gtttggtcaa agagatactt
     7081 ggtcaaatta tctcgaacct tccctttaag atctgcccag tcaaagtcat ctccttgaag
     7141 ataatcttct accgtttggt taatcaattc tgaactttca cggagaatat cgcgactctt
     7201 cttgagataa acaaatccgc gcgtatgaac acgagcccta gccacaattt tcttctcacg
     7261 acggttgact gtaatagcca cgatgaaaat tccatcctct gacaagatct tacggtcacg
     7321 aagaacaaca tttccaacat caccaatggc attcccatca atcaagatat ctcctgctga
     7381 aaccgatcca gctggaacaa agtctccatt ctcgtaagcc atggtcgtcc cctttttagg
     7441 aatgaagatg tgttctggca acatcccaac tgccatggca gccttagcgt gagcatccaa
     7501 ctcacgatac tccccttgga cagggaagag gtacttaggt tgcaaaagat tgatcatcag
     7561 ctgcaaatca cgcacatttc cgtgccctga tacatgtaaa ctttgggtaa tcaatttgac
     7621 aaccccacct gcctgataaa tcatattttc cacacgcgca acaaaggctt ctttagcaat
     7681 agacggagcc gtagcaatat agaccaggtc cccatccttg atttctacat aacgatggcg
     7741 accaatcgac atcttacgaa gtccattgat aggttcaccc atacgacctg tctcaagaat
     7801 aatcaactca tggtcttcaa agcgagacat atctttaggc ttaatcaaga gaatttcgtt
     7861 ggctaaagac aacttcttaa gacgaatcgc tgtgcggacg atattttcaa tatcaaatcc
     7921 tgtcaagacg atacgtcgac ctgttttatc cgcagcgtca aaaatctgct gaatacgaga
     7981 aagattactg gaaacagctg caacgatgat acgaccttcc cagtcagcaa tagtttgggt
     8041 aatttcatcc ctaacttcac tttcactagc cacctgaata ttgctgtctg cattggccga
     8101 atcactgagg agagccagga cgccgtcacg accaatctct gccaaacgag caaaatcagt
     8161 tgcataagat tcactagccg tttggtcaaa tttgaagtca cctgtataaa cgatgcttcc
     8221 ttccgatgtc ttcaagacaa ttcccagact ctctggaacg gagtaagtcg tagggaagaa
     8281 ggaaaccact gtcccaccaa aatcaatctc cgtattctca tcaatgacat ggaaatcatt
     8341 aaatttctta acggcatcat ttcctttgac aaagagcttt gccaactcaa tggtcaactc
     8401 agacccaaat acaggaactt tagcctctgc caagagataa ggtagagcac caatggcatc
     8461 cgcatgcccg tgggtcaaga aaaccccagc aatacggtcg ctattttcaa aaaggtaatc
     8521 catgtttgga atcaccacat cgacccctaa ttgttcattt tcaggatatt ttaaccctac
     8581 attcaaaaca aaaatggact ctccaatttc agcaatgtac atattttttc cattctcacg
     8641 cacaccacca agtgttgtta aactgatatt actcattttt cctccaaaat cttagtttat
     8701 cttgattata gggcaattct accctcttat tctaaaagtt ctgatacttt cagccgaatc
     8761 ttttcctacc attataccat ttttttgatg attttcaaaa tgaaggtctg ttttcaaaaa
     8821 agagttgatt tcccaactct ttcctaactt ctactctttt tccatcaaaa aatcataaat
     8881 ttcttgcacc gtacccagcg ccatcatttc ttggtcttta acggtttgtt tgagattttg
     8941 gtaaatttga ctttctccta tctcccgctt tggcgcctct ttattgactg tcatcacacc
     9001 atcttttatc gtcacaaagc ctagttcttc aaatacttga atcatcttga ccagcaagat
     9061 ttgttgaata ttaagatatg cagccaaatc tttcagcttg tagcgaatat caaactctgg
     9121 gaactggtaa atagtcttgt acaatttagc aaactgatct ctagtcccat aacctgtcag
     9181 ataataggtc ttgtcaatat catttttgaa atagacagca gagaaatgct gttcctgaaa
     9241 aatggtcttc agctgagtaa tatcctctgg aatgtttttt acgacaacag cttcactagc
     9301 cgcaagattt ggcagttctc caggaaaatc caagactgga acaccttctg gcaagactgc
     9361 attttttcca cgaatgttaa aaagttgaac accttccact cgcgcatcca ccatcatcaa
     9421 ctggagggca gtttggccat tccattggtt gacagacaat ttaaccgcta actctagatt
     9481 cttggtttga gaaaactctg tcgcccatct gccttgacca aaggctacca cttcaaaact
     9541 cgcctcaccc ttggaaattt tcagctttag atgggcatta cctgccccca tagtacgagc
     9601 actttcgacc tgaaaattct tgatataaaa aataggtttc tgattatcca ttccaaaagg
     9661 agctaaacgt tcaaaacttt tgaccgtttc caagctaagt gcctccaaat ccaactcttc
     9721 atctaggttt aacttattct tgccaccagc atctgcacct ttttcacgaa cataatcttc
     9781 caaaacctga gataaatctg agagttgctc aacttccagc gtcatacccg ctgcacctgc
     9841 atgacctcca aaggcgatga agaggtctcg atggggatcc agagcttcaa aaatatcgac
     9901 cgcttccaca ctacgagcac tgcccttggc acgaccgtct tctatattaa gaacaatgac
     9961 tgtctgtccc aattcttcca ataaacgacc agccacgatt cctagaaccc caggattcca
    10021 gccttccttg gccaagacct gaaccttctt ctcaggatcc accatggtct tggcttcttc
    10081 atagatagac tgaacgattt ccttgcgctc ttcgtttttc tggtgaatca taagggcaat
    10141 ctcatgcgct tcctcatcat caaatccagt caacaaatca atggcaggat tgggatcatc
    10201 caagcggccc aaggcattca aacgaggagc aatctggaaa ccaaccgttt cttctgttac
    10261 ttcgttggca gcaatcccag ccatgtccag catttcttgc agaccaatgc gctgggtatg
    10321 acccaacatt tccagaccat attgaactaa gatacgattt tcatccgtca gactcaccat
    10381 atctgcaata gttccaatag cgaccaaatc aagcaattcc acttgcactt cttctaacag
    10441 ggcacaagcc aacttgaaag caactccaca accagccaaa tatttaaaag gataatccgc
    10501 atctggatgt tcaggatgga caatagcata agcatctggc agggtttcag gcatggaatg
    10561 atggtctgtc acaatgacat ctactcccat agactgagcc aatgcaatag cctcatgacc
    10621 agcaacccca ttgtccaccg tcacaatcaa ggaaattcct tcttgctcga taaagtattt
    10681 ataaacacta gcattagggc catagccatc ggtaaaacga tttggcaggt aaactcggca
    10741 ctcagcacca agttgttcca aactttcctt cacaatagaa gccgaagtca tgccatccgc
    10801 atcatagtct ccataaacga gaatattttc cccttcttca atagcctgac gaatccgctc
    10861 cactgccttg tccatatcat ggagcagata agcatcatgt aagtcctcca aggaaggttc
    10921 taaaaacttc ttcagacttt cttggtcctg aatccctctc tcaaacaata accgagccac
    10981 ctcaggaccc agtccagcct tcttggctat ctttgtaaaa tccgcatctt ctacctgcag
    11041 ggcaaactgc cattcataag taggtgttat caaaaagaca tccactcttt ctaagaattc
    11101 tattgcttca ttataacatg atttagacgt tttctctatc cagattgctt ttttataaca
    11161 ttttatagta tattgaaact agaatagtac acctctactt ctaaaatatt gttagaaatc
    11221 gatttgactg tcccgatcga tttgtcctgt tcttatttca ctatagtgaa ttttttcaga
    11281 tatggtttga caagaacatt ctttcattgt agaatcgtgt tataaaatat aacacaaagg
    11341 agatctctca tggctttagt agaatttaaa aacgtcgaaa aatattacgg agactaccac
    11401 gcactccgca acatcaatct ccgttttgaa aaaggacaag ttgttgtcct gcttggacct
    11461 tctggctctg ggaagtccac tcttatccgt acgatcaatg gtttagaggc tgttgacaaa
    11521 ggaagtctcc tagtcaatgg gcaccaagtt gctggtgcca gccagaaaga tttggtacct
    11581 cttcgcaagg aagtcggcat ggtttttcaa cattttaacc tttatccaca caaaacggtg
    11641 ttagaaaacg tgacacttgc gcccattaaa gttctaggaa ttgataaaaa agaagctgaa
    11701 aaaaccgccc aaaaatatct ggaatttgta aatatgtgag acaagaaaga ttcctatccc
    11761 gccatgctat ctggtggaca aaaacagcgg atcgccatcg ctcgtggtct tgctatgcat
    11821 ccggaactcc tcctctttga tgaaccaaca tctgctcttg atcctgagac tatcggagat
    11881 gttctagcag ttatgcagaa actggcgcat gatgggatga acatgatcat cgttacccac
    11941 gaaatgggct ttgctcgaga ggttgcggac cgcattatct ttatggccga cggagaagtt
    12001 ttagtagata cgacagatgt cgataacttt tttgacaatc caagcgaacc tcgtgcccaa
    12061 caattcctca gcaaaattat caaccacgaa agtgacaaag tcaaataagg aggcgcctat
    12121 gaaaaaaaat tctttttatc agcattattg attagccttt tcggccttgc tgctgccaaa
    12181 ccagtccagg ctgatactag tatcgcagac attcaaaaaa gaggcgaact ggttgtcggt
    12241 gtcaaacaag acgttcccaa ttttggttac aaagatccca agaccggtac ttattctggt
    12301 atcgaaaccg acttggccaa gatggtagct gatgaactca aggtcaagat tcgctatgtg
    12361 ccggttacag cacaaacccg cggccccctt ctagacaatg aacaggtcga tatggatatc
    12421 gcgaccttta ccatcacgga cgaacgcaaa aaactctaca actttaccag tccctactac
    12481 acagacgctt ctggattttt ggtcaataaa tctgccaaaa tcaaaaagat tgaggaccta
    12541 aacggcaaaa ccatcggagt cgcccaaggt tctatcaccc aacgcctaat tactgaactg
    12601 ggtaaaaaga aaggtctgaa gtttaaattc gtcgaacttg gttcctaccc agaattgatt
    12661 acttcccttc acgctcatcg tatcgatgcc ttttccgttg accgctctat tctgtctggc
    12721 tacactagta aacgcacaga actactagat gatagtttca agccatctga ctacggtatt
    12781 gttaccaaga aatcaaatac agagctcaac gactatcttg ataacttggt tactaaatgg
    12841 agcaaggatg gtagtttgca gaaactttat gaccgttaca agctcaaacc atctagccat
    12901 actgcagatt aaggaggaca ccccatgata gatttatcat cttggacagc ctattttcag
    12961 gattttggac aatttttcaa tggtttcctc tttactcttg ccctagcgat tggatccttt
    13021 atcctcgcca tggtcttagg aatcttcttt ggtgccatgt caaccagtaa acgtccaatt
    13081 ttacgcattt tagctcacat ctttgtggaa ttttaccaaa acactcccct cttggtgcag
    13141 tttgttattg ttttttatgg cctacctctt atcagtgacc atactatcat gattccaatt
    13201 tattggacag ctgttctctg catgggactc tatcacggcg cttatatcgc tgaggttatt
    13261 cgttcaggga ttcagtctat tcctagtggt cagatggagg ccgccttgtc gcaaggtttt
    13321 acctatatca gtgccatgcg cttgattatc ttgcctcaga ccttccgcat cattctccct
    13381 ccattgacca accaaattgt taacctcatc aagaacacct ctactgtagc tatcatctct
    13441 ggagtagact tgatgtttgt gactaagtct tggtcggctc tcaacggaaa ctatatccca
    13501 gcctttttag gtgctgctct tctctacttt gctctatgct tccctgttgc ccagtttggt
    13561 cgcaagatgg agcaagccaa caaaaaagcc tattcacttt aggaggttac tatggaatct
    13621 attttagaag ttttaacccc agataaccta gtctttatct ttaaaggatt tggcttgacc
    13681 ctctatattt ctctgattgc catcatcctc tctactatca tcggtacggt gctagctgtc
    13741 atgagaaatg gcaaaaatcc tgtcttacgc attatttcca gtatttatat cgagtttgtg
    13801 cgcaacgttc ccaaccttct ctggattttt actatctttt tggtgttcaa aatgaaatcc
    13861 acaccagcag gtattacagc ctttactctc tttacatcag cagccttggc tgagattatt
    13921 cgaggcggtc tcaataccgt agacaaggga cagtacgaag caggaatgtc acaaggcttc
    13981 acctcagccc aaatcctcta ctacatcatt ctcccacaag ccatccgcaa aatgctacca
    14041 gccatcattt ctcagtttgt taccgtgatt aaggatacca gtctcctcta ctctgttatc
    14101 gccctacaag aactctttgg agccagccaa attctcatgg gccgttattt cgaaccagag
    14161 caggtcttca gtctttgcat cctgattgcc ctcatctact tcagctttaa cctagcaatt
    14221 tctagcctgt ctcatatgct agccaaacgt tggcaacaag ctgcaga
//
LOCUS       DAVBCW010000048        14037 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVBCW010000048 DAVBCW010000000
VERSION     DAVBCW010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14037
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.048"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            81..878
                     /locus_tag="VQ514_001208"
     CDS             81..878
                     /locus_tag="VQ514_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HET2499964.1"
                     /translation="MVSSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            1026..1148
                     /locus_tag="VQ514_001209"
     CDS             1026..1148
                     /locus_tag="VQ514_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HET2499965.1"
                     /translation="MTCDFKFETLQLHAGQVVAPATKSRAVPIYQTTSFIFDDT"
     gene            1170..2210
                     /locus_tag="VQ514_001210"
     CDS             1170..2210
                     /locus_tag="VQ514_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HET2499966.1"
                     /translation="MRKPGNIYTRITNPTTAALEGGVEALATASGMTAVTYTILALAH
                     AGDHVVAASTIYGGTFNLLKEPLPRYGITTTFVDVDNLEEVEAAIKDNTKLVLIETLG
                     NSLINIPDLEKLAEIAHKHQIPLVSDNTFAIPYLINVFSHGVDIAIHSATKFIGGHGT
                     TIGGIIVDSGRFDWTASGKFPQFVDEGPSCHNLSYTCDVGAAAFIIAVRVQLLRDTGA
                     ALSPFNAFLLLQRLETSSLRVERHVQNAETIVDFLVNHPKVEKVNYPKLADSPYHALA
                     EKYLPKDVGSIFTFHVKGGEEEARKVIDNLEIFSDLANAADAKSLVVHPATTTHGQLS
                     EKDLEAAGVTPN"
     gene            2381..3655
                     /locus_tag="VQ514_001211"
     CDS             2381..3655
                     /locus_tag="VQ514_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HET2499967.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMKQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHEALLAKDKEV
                     QLLENQLATLRLEHENQLQKTLSDLEKERDQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKDFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            3665..4543
                     /gene="truB"
                     /locus_tag="VQ514_001212"
     CDS             3665..4543
                     /gene="truB"
                     /locus_tag="VQ514_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HET2499968.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEIILGYSTTTEDASGEVVAETPVLSPLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALTLEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            4811..5086
                     /locus_tag="VQ514_001213"
     CDS             4811..5086
                     /locus_tag="VQ514_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HET2499969.1"
                     /translation="MEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQSVMANLSQGL
                     SLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            5194..5832
                     /gene="udk"
                     /locus_tag="VQ514_001214"
     CDS             5194..5832
                     /gene="udk"
                     /locus_tag="VQ514_001214"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HET2499970.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            5960..7300
                     /locus_tag="VQ514_001215"
     CDS             5960..7300
                     /locus_tag="VQ514_001215"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET2499971.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESVVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            7278..7490
                     /locus_tag="VQ514_001216"
     CDS             7278..7490
                     /locus_tag="VQ514_001216"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HET2499972.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            7487..8362
                     /locus_tag="VQ514_001217"
     CDS             7487..8362
                     /locus_tag="VQ514_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET2499973.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLESLQVTIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            8355..9170
                     /locus_tag="VQ514_001218"
     CDS             8355..9170
                     /locus_tag="VQ514_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HET2499974.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHSVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            9163..9594
                     /locus_tag="VQ514_001219"
     CDS             9163..9594
                     /locus_tag="VQ514_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET2499975.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIETFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            9601..11268
                     /gene="recN"
                     /locus_tag="VQ514_001220"
     CDS             9601..11268
                     /gene="recN"
                     /locus_tag="VQ514_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HET2499976.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRTIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLDLNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNKMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            11270..11998
                     /locus_tag="VQ514_001221"
     CDS             11270..11998
                     /locus_tag="VQ514_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HET2499977.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQPQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYSRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEAADLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            12077..13900
                     /gene="lepA"
                     /locus_tag="VQ514_001222"
     CDS             12077..13900
                     /gene="lepA"
                     /locus_tag="VQ514_001222"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HET2499978.1"
                     /translation="MNLEELKKRQEKIRNFSIIAHIDHGKSTLADRILEKTATVSSRE
                     MQAQLLDSMELERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
BASE COUNT         4152 a         2566 c         3349 g         3970 t
ORIGIN      
        1 ttttatgaaa tacagaaaac gcttcctaaa acctaaagtt tgtgatataa ttatcaagaa
       61 aaaagattta ggagtttatt atggtttctt cagaatttat ctcaaagatt gaatttgctt
      121 gcaataagaa agaaagtctt tatagtcaaa gcaaatttaa gtatgcgatt cgttcgatgt
      181 tcgcaggtgc atttttaacc ttcagtactg ctgcaggtgc agttggggct gacttgatta
      241 ataaaattgc accaggtagt ggacgcttcc tctttccatt cgtttttgct tggggcttgg
      301 cctacattgt ttttttgaat gccgagttgg tcacttcaaa catgatgttc ttgactgctg
      361 gtagtttctt aaaaaaaatc tcttggagaa aaacagctga gattttacta tactgtacct
      421 tgttcaacct tatcggagcc ttgatagcag ggtggggctt tgctcattcg gcagcctatg
      481 cgaatctgac acacgatagt ttcatctcag gtgttgttga gatgaagtta ggccgctcaa
      541 atgaattggt cttgcttgag gcgattttgg caaatatttt cgtaaatatt gcgattctgt
      601 catttatttt ggtcaaagat ggtggtgcca aactttggct tgtgttgtca gctatttaca
      661 tgtttgtatt cttaacaaac gagcacattg cggcgaactt tgcttctttc gcgattgtga
      721 aattcagtgt tgctgcggat tcaattgcca acttcggtgt tggaaatatg cttcgccact
      781 ggggtgtgac tttcatcgga aactttatcg gaggaggcct cttgatgggt cttccatatg
      841 ccttcctcaa taaaaacgaa gatacttatg tagattaaga aaatgagcac gattgagtcg
      901 tgcttttttc attttcaaaa taaggtaata gctatttctt atatcaaaat atagaaaact
      961 gatatttgta aactataact caaggtgcta caatatcctt aataaaatga tatggaggtc
     1021 accttatgac ttgtgatttt aaatttgaaa ctctacaact acatgctggt caagttgtgg
     1081 ctccagctac taagtctcgt gcagtgccga tttatcaaac aacatctttt atttttgatg
     1141 acacgtagga aggtgctgat ctgtttgcct tgaggaaacc agggaacatt tatactcgta
     1201 tcaccaatcc tacaacagct gcccttgaag gtggtgttga agcgctagca acagcatcag
     1261 gtatgactgc agtgacttat acgattttgg cgcttgccca tgctggtgac catgtagtgg
     1321 ctgcttcgac tatttacggt ggaaccttca atcttttgaa agaacccctt cctcgttatg
     1381 gtatcacaac aacctttgtc gatgttgata atttggagga agtagaagca gctatcaaag
     1441 acaataccaa gcttgtcttg attgaaacct tgggtaactc cttgattaat attccagacc
     1501 tggaaaaact ggcagagatt gctcataaac atcaaatccc acttgtgtca gacaatactt
     1561 ttgcaatacc ttatttgatt aacgtcttct ctcatggcgt tgacattgcc attcactctg
     1621 cgactaagtt tatcggtggg catggtacaa ctattggagg aataattgtc gatagtggtc
     1681 gttttgactg gacggcttca gggaaattcc ctcaatttgt tgacgagggt ccaagctgcc
     1741 acaatttgag ttatacttgt gatgtgggtg cagcagcctt tattatagct gttcgagttc
     1801 aattgcttcg tgatacaggt gcagccttgt caccattcaa tgctttcctc ttgctacaaa
     1861 gacttgaaac ctcttcactt cgtgtggaac gccatgtaca aaatgctgag acaattgttg
     1921 attttcttgt caaccatcct aaggtagaaa aggtaaatta tccaaaactt gcagatagtc
     1981 cttatcatgc cttggctgag aaatacttgc caaaagatgt cggttcaatc tttaccttcc
     2041 atgtcaaagg tggcgaggaa gaagcacgca aggtcattga taatttagaa atcttttctg
     2101 accttgcaaa cgcggcagat gctaaatcgc ttgttgtcca tccagcaaca accactcacg
     2161 gtcaattgtc agaaaaagac ctagaagcag caggtgtcac accaaactaa attcgtttgt
     2221 caatcggtct tgaaaatgta gaagatttga ttgaagactt gcgcttggcc ttggaaaaaa
     2281 tttaaagtaa aagaagataa acagtgggct tcgactcact gtttttgatt ttccctcagg
     2341 catgatataa tggttaaaga agtctagaaa gaggaacgat atgaacgaaa tcaaatgtcc
     2401 caactgtggg gaagtcttta cagtaaatga gagtcagtat gccgaactct tgtcccaagt
     2461 gagaacggca gagtttgata aggaactaca cgataggatg aagcaggaac tggccttggc
     2521 tgagcaaaag gccatgaatg agcaacagac taaactggct cagaaggatc aagaaattgc
     2581 gcaattacag agtcagatcc aaaactttga tacagaaaaa gaattggcca agaaagaggt
     2641 tgaacagaca agccatgagg ctctcttggc taaggacaag gaagtacagc tcttagaaaa
     2701 tcagttggct accttgcgtt tggagcatga aaatcaactg caaaagaccc tttctgacct
     2761 agaaaaagaa cgggatcagg ttaaaaacca actacttttg caggaaaagg aaaatgaatt
     2821 atctttggct tctgttaagc aaaactacga agcccagctc aaggcagcta gtgaacaagt
     2881 cgagttttat aaggatttta aggctcaaca atctacaaaa gcgattgggg aaagcctaga
     2941 acagtatgca gagagtgagt ttaacaaggt tcgtagtttc gcctttccaa atgcttactt
     3001 tgagaaggat aacaaggtct cttcgcgtgg gtctaaaggg gactttatct tccgtgagtg
     3061 tgatgaaaat ggagttgaaa tcatttctat catgtttgag atgaaaaacg aagcagacgg
     3121 aacagagaag aagcacaaga atgcagattt ttacaaggaa ttggacaagg accgtcggga
     3181 gaagaactgt gagtatgccg ttttggtgac catgcttgag gccgataatg actactttaa
     3241 cacagggatt gttgacgtca gccacgagta tgaaaaaatg tatgttgttc gtcctcaatt
     3301 ctttatccag ttgattggtc tcttacgtaa tgcggcgcta aattccctaa aatacaagca
     3361 ggagttggcc ttggttcgcg agcaaaatat tgacattacg cattttgagg aagacttgga
     3421 tgcctttaag ctagcttttg ctaagaacta taattcagct tcgactaact ttggaaaagc
     3481 tattgatgaa atcgacaagg ccatcaaacg catggaagag gttaagaaat tcctgactac
     3541 atctgaaaac caactccgtc tagctaacaa caaattggaa gatgtctctg ttaaaaaatt
     3601 gacccggaaa aatccaacaa tgaaagcgaa gttcgaagca ctgaaggggg agtagaaagc
     3661 aaaaatgaac ggtattatca acttaaaaaa agaagcagga atgacctcgc atgatgcggt
     3721 ttttaaactg cgtaagattt tgggaaccaa gaaaattggt catggtggaa ccttggatcc
     3781 ggatgtggtg ggtgttttgc cgattgcggt tggcaaggcg acacgcatgg tcgagtttat
     3841 gcaggacgag ggtaagatct atgaggggga aatcattctg ggctattcca cgacgactga
     3901 ggatgctagt ggggaagtgg tcgcagaaac ccctgttttg tctcccttgg atgaaaagct
     3961 tgttgatgaa gcgattgcta gcttgactgg gcctattact cagattcccc ctatgtattc
     4021 ggcagttaag gttaatggtc gcaagctcta tgagtatgcg cgtgctggtc aggaagtgga
     4081 gcgtccagaa cgtcaggtga ccatttatca atttgagcga acaagtccga tttcttatga
     4141 tggccaactt gcccgattca cttttcgtgt aaaatgcagt aaagggactt acatccgtac
     4201 tttgtcagtt gatttgggtg aaaagcttgg ttatgcggct catatgtccc atttgactcg
     4261 tactagtgct gctggcttac aattagaaga cgctcttacc ttggaggaaa ttgctgaaaa
     4321 agtagaggct gggcaattag attttctcca tcctttagag attgggacag gtgaccttgt
     4381 caaagttttc ctaactccag aagaggctac agaagttcgc tttggtcgtt ttattgagct
     4441 agaccaaacg gacaaagaac tggctgcctt tgaagatgat aaattgttag ccattctaga
     4501 aaaacggggc aatctctata agccaaggaa ggtttttagc tagatcgttt aggaataaaa
     4561 atcgggtgat agataacaat tgcttgataa acccccatac taatagtaga atggttttgg
     4621 gaattataat attccaattg ttgcgagttg tagatactca aataatctat atagaaattt
     4681 agaggtgtga aatgaagcaa cttaaaattc tttcagataa atatttagag tccatgacag
     4741 gttctggtgg gaacttaggt ctaggatttg gtgtgataat tccatgatgt gaaatgagtt
     4801 tcgagaaagg gtggagcaac ttcttcaaca aaaagaaata aatgaaaata gtgagttgag
     4861 tcacctgttt cgtcttgcta tacaaaattt agacagaaat gaaaaatacc aatcggtcat
     4921 ggccaatttg agtcaagggt tgtcacttta cctcatgacg catcattacc aggcacctaa
     4981 gtctgtcatt gattttggtt tatggattgc caaagctcct agccaggaaa gagggagact
     5041 ggctttcttg cagatgcttg ctcaaaccct acagggcttt cgttaaaact gtcgaacatc
     5101 agagggaaga ctctggtgtt ttttgctgat gagactataa attttgtaaa aaatgtgata
     5161 gaatagacga cggataaaat aacggaggat agcatgcaaa atagaccaat cattattgga
     5221 gtgacaggtg gttctggtgg tggtaaaacc agtgtttcaa gagccatttt atcgcatttt
     5281 cctgatgaaa agatttccat gattgagcat gattcatact acaaggatca gtctcatttg
     5341 acctttgaag agcgtgtcaa aaccaactat gaccatcctt ttgcctttga tacagacttg
     5401 atgatcgagc agattaagga attgttggca gggcgtccgg tggacatccc gacctacgac
     5461 tatacagagc atacacggag tagcaagacc tatcgtcagg agcctcaaga tgtctttatc
     5521 gttgagggca ttttggtctt ggaggacaag cgtctgcgcg atttgatgga tatcaagatt
     5581 tttgtggata cggatgacga tgtgcgtatt attcgtcgta tcaagcgtga tatggaggaa
     5641 cgtggccgta gccttgatag cgttattaac cagtacttag gtgtggtcaa accaatgtac
     5701 caccagttta tcgagtcaac taagcgttat gctgatatcg tcattcctga aggggttagc
     5761 aataccgtgg ctatcgacct gttgacgacc aagattgcaa agattttgga agaagctcga
     5821 aacagcaaat aatcacatgt ggaggccttg cctccttttt ctatttttcc ctcataatgg
     5881 tacataaatg gagattttta ggcatatttt tggtataata atacccataa aagagcaagt
     5941 aaatgaatag taggtggaga tggaaaagta tttatcggta acaactttga ccaaatatct
     6001 gaaaatgaaa ttcgataaag acccatactt ggaacgggtc tatttaactg gtcaagtttc
     6061 caactttcgt aaacgaccta ctcaccaata tttctcccta aaggatgacc atgcagttat
     6121 tcaagcgacc atctggtctg ggatttatca gaaattaggg tttgacctgg aagaaggaat
     6181 gaagatcaat gtgattgggc gtgtacaggt ctatgaacca agtggtagct actccatcat
     6241 cattgaaaaa gctgagcctg atggggttgg ggcgcttgcg attcagtttg aacaacttaa
     6301 gaaaaaattg acagaagaag gcctgtttca agaacgcttc aagcaagctc taccccaatt
     6361 ttctaagaga attggggtag taaccagccg tagtggagcc gttattcgag atattatcac
     6421 gaccgtcagc aggcgattcc caggtgttga catccttctt tatcctacca aggttcaagg
     6481 tgaaggggct gcggaggaaa ttgctagaaa tattgcgcgt gctaatcaac gggacgattt
     6541 ggatttgctc attattggtc gtggaggtgg ttctattgag gatctctggg cctttaacga
     6601 agaaattgtg gtacgagcta tttttgaatc tcgtttgcca gttatctcta gtgtggggca
     6661 tgagacggat gtgaccttgg cagattttgt ggcagatcga cgcgctgcaa cgccaacagc
     6721 ggcggctgaa ctggcaacac ctgtgaccaa gttggatgta ttagctcatt tgcaaaatca
     6781 ggaaaaacgg atggcaacgg cagtccgaaa tgttctatct aagaaacaag aggctttgaa
     6841 aaaatgcagt cagtctgtta tctttagaca acctgagcgc ttgtatgacg gttatttgca
     6901 acgcttggac caactgcaac tgcgtttgaa acaaagtttg cgaactcgga tttctgataa
     6961 caaacaatta gttcaagcaa gaactcatca attagtacaa ttatcacctg ttaccaaaat
     7021 ccaacgctat caagaccgtt taggacagtt ggacaagctc ttaggtagcc aaatggcgtt
     7081 agtttatgac gccaaggttg ctgaggccaa gcgactttcg gaagctttgc tcatgttgga
     7141 tactagccga atcgtggcgc gtggttatgc tattgtcaaa aaagaagagt ccgttgtaga
     7201 ttcggttgag agtttgaaga aaaaagacca agtaacgctt ttgatgcgag atggtcaagt
     7261 agaattagag gttaaagatg tcaaaacaaa agaaatttga ggaaaatcta gcagaactgg
     7321 aaaccattgt ccaaagtttg gaaaatggtg aaattgctct ggaagatgcg attactgcct
     7381 ttcaaaaggg catggtcttg tcaaaagagc tccaagctac gctggacaag gctgaaaaga
     7441 ccttggtcaa ggtcatgcaa gaagacggaa cagaaagtga ttttgaatga aaaagcaaga
     7501 aaaattagct cttgtcgagt cggctttgga agatttttat ggagaccagc agtttgcctc
     7561 tagtttacgg gagtctgttc tctattctat tcatgctggt ggcaagcgta ttcggccttt
     7621 tctcttgtta gaagttctgg aatccttgca ggttaccatc aaacctgctc acgcgcaggt
     7681 agctactgcc ttggagatga ttcatacagg gagcttgatt cacgatgacc ttcctgctat
     7741 ggatgatgac gattatcgaa gagggcggtt aaccaatcac aagaaattcg gtgaagctat
     7801 ggccattttg gctggagatg ccttattctt agacccatat gccttgattg cgcaggcaga
     7861 tttgccaagt cagatcaagg tggacttgat tgccaactta tcccttgctt caggtagtct
     7921 gggtatggtg gcagggcaag ttttggatat ggagggcgaa caccagcact tgtctctgga
     7981 agaacttcag actattcatg ccaataagac tgggaagtta ctagcctatc ccttccaagc
     8041 ggcagctatt atagctgaat tgtcacctga aatgcaggtg aagctgaaaa ctgtgggtga
     8101 attgattgga cttgcttttc aagtcagaga tgatgtactg gatgtgacag ctagttttga
     8161 ggaaatcggc aagacacctc aaaaggatct gcaggcagaa aaatcaacct atcctgcctt
     8221 gttgggcttg gaagagtcca ttgccttttg taaccaaacc ctggatcaag ctaatgaaaa
     8281 attagaagaa attgcccagc agcttccctt tgaaacagaa tcgattgtaa gtgtagtaga
     8341 aagtttgaga atcaatggct aaggaaagag tggatgtact agcttataaa caggggttgt
     8401 ttgaaacgag agagcaggcc aagcgaggtg tgatggctgg tctagtcgta gcagtcctta
     8461 atggagaacg gtttgacaag ccaggagaga aaattccaga tgacaccgaa ttaaaactca
     8521 agggggagaa actcaagtat gtcagccgtg gtggtttgaa actggaaaag gccttgcagg
     8581 tctttgattt gtcggtggat ggcgcgacta cgattgatat cggggcctct actggaggtt
     8641 ttaccgatgt catgctacag aatagtgcca agttggtctt tgcagtcgat gttggtacca
     8701 atcagttggc ttggaaatta cgccaagacc cacgagttgt cagcatggag cagttcaatt
     8761 ttcgctatgc tgaaaagact gatttcgagc aggagccgag ctttgccagt attgatgtga
     8821 gtttcatttc ccttagtctg attttgccag ccttgcacag tgtcttggct gatcaaggtc
     8881 aggtggtagc acttgtcaaa cctcagtttg aggcaggacg tgagcagatt gggaaaaatg
     8941 gaattattcg agatgctaag attcatcaga atgtccttga atctgtaaca gctatggcag
     9001 tagaggcagg tttttcagtc cttggcttgg acttttctcc catccaaggt ggacatggaa
     9061 atattgaatt tttagcgtat ttgaaaaaag aaaagtcagc aagcaatcag attcttgctg
     9121 agattaaaga agcagtagag agggcgcata gtcaatttaa aaatgaataa aaaagaaaga
     9181 cttgaaaaaa ttagaagatt ggtgacagat tatcaaatcg gcacgcaaga agaaattgta
     9241 gaacatttga aagaagcagg tatcactgcc actcaggcga cggtatcccg agatatcaaa
     9301 gagttaggta ttgtcaaaat tcctttgaga gacaacacct atgtctacga attgccaaaa
     9361 tcaatcgtaa aaagtctgca actggctgaa gacaatatcg aatcggctga attgatggat
     9421 aagatgatca atctccaagt tattccagga aatacggctt ttgtaaaagc tcagttaatc
     9481 gagacttttg cagacaagat ttttagctgt ttgactgatg atagctcgat tttagtcatt
     9541 gccagaagtg gaagtctagc agaggaaatc tttgaacaag taaaaaattg gtaggtctat
     9601 atgttacttg aaatttcgat aaaaaacttt gccattattg aggctatttc cctcaatttt
     9661 gaaaagggga tgactgtcct gactggtgaa acgggtgcag ggaagtcgat tatcattgat
     9721 gccatgaata tgatgttggg agctcgtgcg acgacagatg ttattcgtca tggtgcacca
     9781 aaggcagaga ttgaggggct tttctcagtt gagaatagtc gtcttttaca ggaaattttt
     9841 gatgagcaag gtttagaatt aggtgatgaa attatcatcc gtcgggaaat cttgcaaaat
     9901 ggtcgtagta tcagccgtgt aaatggtcag atggttaatc tgtctgtttt gcgaactatt
     9961 gggcaacatc ttgtagatat tcatggtcag catgaccatg aggagttaat gcgtccccaa
    10021 ctgcatatcc agatgttgga tgaatttggt gatgccgctt tttgggactt gaaagaaacc
    10081 tatcaaacga gttttgatgc ctatcggaaa atgcgcaagc aggttctgga agtcaagaaa
    10141 aaccaacagg aacacaaggc acgtatcgaa atgttggaat ttcaaatggc agagattgag
    10201 gcagcaaact tgcaggctgg agaagacttg gatctcaatc aagagcgaga taaactcctc
    10261 aaccacaaaa atattgcgga tacactgacc aatgcctaca gtatgttgga caatgaagat
    10321 ttttcaagtc tggccaatgt tcgttcagcc atgaatgaca tggaaagtgt cgaagagtat
    10381 gaccctgaat accgtgaaat ttcaagctct ctgtctgaga cctactatgt tttagaagac
    10441 attagcaaac gtttggaagc tatcattgag gaccttgatt ttgatggcaa tcgcctcatg
    10501 caggttgaga atcgtttgga cctccttcat actattactc gtaagtatgg tgggactgtt
    10561 gatgatgttt tgctttattt tgccaagatt acggaagaat acaatctctt gacaggcaat
    10621 aatctttcgt ctgaggacat ggaagcagag cttaagaagt tggaagtcaa tcttgtcaat
    10681 ttggcaggtc aacttgcttc tgctcgtcat aatttggctc agcaactcga agctgagatt
    10741 aaacaagaac tgcaagatct ttatatggaa aaagcccagt ttcaggttcg ttttagtaag
    10801 ggaaaattca gtcgtgaggg aaataaaatg gttgagtttt atatttcaac caaccctgga
    10861 gaagacttta aacccttggt taaggttgct tctggagggg aattatctcg tctcatgtta
    10921 gccattaagt ctgccttttc acgtaaagaa ggcaagacta gcattgtctt tgatgaggtg
    10981 gatacgggag tttcaggtcg tgttgctcaa gctattgctc agaaaattca taaaattggt
    11041 cagcatggtc aggttttggc tatctcccat ttgccacaag taattgcgat tgcagattat
    11101 caattcttta ttgagaagat tagtaatgac cattcaacgg tttcgactgt tcgtctcttg
    11161 acggtcgaag agcgagtgga ggaagttgcc aagatgttgg caggtgatga tgtgacagaa
    11221 gcagccctga cgcaagcaag agaattgttg agaaacaggg agaaataaga tgacagatta
    11281 ttatgtaatt ggagatgttc acggaaaagc tgggatgctg gaagaccttc tcaaaacctg
    11341 ggatggtcag ccccagttgc tctttctagg tgatttgatt gacagaggtg aggatagtca
    11401 ccgtgttctt gaaatggtca aggacttggt ggacaatcaa ggggctatct gtttgtccgg
    11461 aaaccacgag tatatgtttt tgacttggct agatgaccca gaagaaagtt atgaccatta
    11521 tagtcgcaat ggtggagata caaccattaa ctctatccta ggtcgtccct tggatgcacc
    11581 agttgatgga gtagaagatg ccaagcgggt tgccgctgaa gcggcagact tggtcgaatt
    11641 cattcgtcaa atgccatttg tagtagagac agacaagtat atctttgttc acgcaggtat
    11701 tgatttgacc ttggacgact ggcatgaaac aacagattat aaaaaagtat ggcttagaaa
    11761 accattccac ggagctgaaa atcatactgg aaaaaccatt gtcttcggtc atacaccggt
    11821 ctatggtttg ttacagcaag aacgcggtac atctgagctt tggactacag aagatggcaa
    11881 gattggaatg gatggaggag ctgtttatgg tggtgtcctt cacgggattg tctttacaga
    11941 ccaaggaatg acagaacacc actttatcga aaatgacggc tttgtcgctg aagattagta
    12001 ctcctagcag ggtatggtct tgtcaaaata tcaaaaacaa tttataataa atagataccc
    12061 tgaaaggaag agaatcatga acttagaaga attgaaaaaa cgacaggaga agatccgtaa
    12121 cttctctatt atcgcccata ttgaccacgg aaaatcaact ctagcagacc gcattttgga
    12181 aaaaacagcg acggtttcaa gtcgtgaaat gcaggcccag cttttggata gcatggagct
    12241 agagcgggaa cgtggaatta ctattaagtt gaatgccatc gagttgaatt acactgcaaa
    12301 agatggggaa acttatattt tccacttgat tgacacacca gggcacgttg actttaccta
    12361 tgaagtttca cgttcgctag ctgcctgtga gggtgctatt ttggtggtcg atgcggctca
    12421 aggaattgag gctcaaactc ttgccaacgt ttatctggct ttggataatg atttggaaat
    12481 catgcctatc attaataaaa ttgacctgcc ggctgcagat ccggagcgcg tgcgtacaga
    12541 gattgaagat gtgattggtt tggatgccag tgaagcagtt ttggcttctg ccaaggctgg
    12601 tattgggatt gaagaaatcc tcgagcaaat tgtagaaaaa gtaccagcac caacgggtga
    12661 tgtgacggcg ccacttaagg ccttgatttt cgactctgtt tacgatgctt accgtggggt
    12721 tatcctccaa gtgcgtgtca tggacggagt ggtcaaacct ggtgataaga ttcagctcat
    12781 gagcaatagt aagacctttg atgtggccga agtcggtatt tttacaccaa aagcggttgg
    12841 tcgtgatttc cttgcgactg gtgatgttgg ttacattgcg gcgtctatca agacggttca
    12901 ggatactcgt gtgggtgata ccgttacctt ggcaaccaat cctgcggcag aaccattaca
    12961 tggttataag cagatgaatc ctatggtctt tgcgggtctc tacccaatcg aatcaaacaa
    13021 gtacaatgac ctgcgtgaag cgcttgaaaa attgcaactg aatgatgcta gtcttcagtt
    13081 tgaaccagaa acatctcagg cacttggatt tggtttccgt tgtggatttc ttggacttct
    13141 ccatatggat gttatccagg aacgtttaga gcgtgagttc aatattgacc tcatcatgac
    13201 agctccgtct gttatttaca aagttaattt gaccgacggt gagtctatgg atgtgtctaa
    13261 cccatctgag tttccagacc caactaagat tgcgaccatt gaagagcctt atgtcaaggc
    13321 gcaaatcatg gtaccacagg agttcgtcgg agcagtaatg gagctagctc agcgtaagcg
    13381 tggggacttt gtgactatgg actatattga tgataaccgt gtcaatgtta tctatcaaat
    13441 tcctcttgct gaaattgtct ttgacttctt tgataaactt aagtcttcga cacgtggtta
    13501 tgcaagcttt gactacgaat tgtcagaata tcgcccatct aagctggtga aaatggatat
    13561 tcttctcaat ggagataagg tggatgccct cagctttatc gttcacaagg actttgccta
    13621 cgaacgtggg aaactcatcg ttgataaact caagaaaatc atccctcgcc aacaatttga
    13681 agttccaatc caagcggcta ttggacacaa gattgtcgct cgtactgata tcaaggccct
    13741 tcgtaagaac gtacttgcta aatgttatgg tggtgacgtt tctcgtaagc gtaaactcct
    13801 tgaaaaacaa aaagctggta agaaacgcat gaaatccatc ggatcagttg aagttccgca
    13861 agaagccttc ctcagcgtct tgagtatgga tgaagaataa gttatcagaa atgcctttcc
    13921 ttgttcacag gaaatttata taagaatcaa tagtaaagca ggcttagatg gtctgctttt
    13981 ttagttttta tactcttcga aaatctcttc aaaccacgtc agcgtcgcct taccgta
//
LOCUS       DAVBCW010000049        14005 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVBCW010000049 DAVBCW010000000
VERSION     DAVBCW010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14005
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.049"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(114..1964)
                     /locus_tag="VQ514_001223"
     CDS             complement(114..1964)
                     /locus_tag="VQ514_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HET2499979.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMVDFWGLSASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVARAVDEAKVPKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(2032..3171)
                     /locus_tag="VQ514_001224"
     CDS             complement(2032..3171)
                     /locus_tag="VQ514_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2499980.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKGKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDAEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGGLILKIDAHSKVTESFVMNNVAVIQQGEFVCGGPRP
                     TIVKGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREHGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLSLLTLLTLLKHK
                     NGFLIVMPFLLFSIHFAYGLGTIVGLIRGFKWKKEYKGTVIYLEK"
     gene            3229..4395
                     /locus_tag="VQ514_001225"
     CDS             3229..4395
                     /locus_tag="VQ514_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HET2499981.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTRLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TAMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPAVAALLLIDILMTVALFTVDRADKIL"
     gene            4534..4860
                     /locus_tag="VQ514_001226"
     CDS             4534..4860
                     /locus_tag="VQ514_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET2499982.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWTLYTDTINGIIEGS
                     IRHDKN"
     gene            4847..5440
                     /locus_tag="VQ514_001227"
     CDS             4847..5440
                     /locus_tag="VQ514_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HET2499983.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEVAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVARFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            5433..6344
                     /locus_tag="VQ514_001228"
     CDS             5433..6344
                     /locus_tag="VQ514_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HET2499984.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHTLTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            6407..7471
                     /locus_tag="VQ514_001229"
     CDS             6407..7471
                     /locus_tag="VQ514_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499985.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADLDTPIGK
                     NTQVEEEVSQAEVELESQQEEKIETPEGGEARTEIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(7543..8403)
                     /locus_tag="VQ514_001230"
     CDS             complement(7543..8403)
                     /locus_tag="VQ514_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HET2499986.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHIYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTETDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDGSKPEDYEAIARLDYAKFLEVLPPSFYHQLDANQIEVQPILD
                     KDFKTLAQEK"
     gene            complement(8525..9511)
                     /locus_tag="VQ514_001231"
     CDS             complement(8525..9511)
                     /locus_tag="VQ514_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HET2499987.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEELWFKIDEENEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(9783..11258)
                     /locus_tag="VQ514_001232"
     CDS             complement(9783..11258)
                     /locus_tag="VQ514_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HET2499988.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(11362..12285)
                     /locus_tag="VQ514_001233"
     CDS             complement(11362..12285)
                     /locus_tag="VQ514_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2499989.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(12299..13228)
                     /locus_tag="VQ514_001234"
     CDS             complement(12299..13228)
                     /locus_tag="VQ514_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2499990.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(13547..13897)
                     /locus_tag="VQ514_001235"
     CDS             complement(13547..13897)
                     /locus_tag="VQ514_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499991.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKAFADDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETADKDVLENSQEELQKAVLFLADEKGSEHTEAELI
                     DNLKEVIAKLKANA"
BASE COUNT         4187 a         3024 c         2582 g         4212 t
ORIGIN      
        1 acggcaaggc gacgttgacg tgatttgaag agattttcga agagtataag tctctacata
       61 ataaaacgca tattaccaag gttatttaac ttgataatat gcgtttttct tttttattca
      121 atgtgggttg tttgattagc aaaggcgata atagcttgct tcaactcatc tccactgaga
      181 gtgcggaact ctccaatctt ttgattgatg gattctaaag gcatgacatt aaccttacca
      241 acgaaaatct tatccataac ttgattatca acgagttcgg ttgataccaa gagttcttcg
      301 tagagttttt cacctgggcg gattccaact tcaacgattg gaatttcact ttcagtgtgg
      361 ccacttagaa gcaccatctt cttggccaag tcataaatct tgactggttt gcccatatca
      421 aggataaaga cttccccatc tttggcataa gcaccagcat ggataaccag acggctagct
      481 tctggaatgg tcataaagta acgggtcatg cggaagtctg tcaccgttac aggcccacct
      541 tcagcaatct gacgttcaaa gactggaatg acactaccac ggctaccaag aacattccca
      601 aaacgaactg cacagtaggt tgattggcta cgttggttaa agccagtgac aatcaactcc
      661 gccacgcgct tggttgctcc cataacattt ggtggattga ctgccttatc tgtcgaaatc
      721 ataaccatct taggcacctt ggcttcatca acggctctag caacattgta agttccacgg
      781 atattgtttt tgaaggcttc ttttggattg cgctccatca taggaacatg cttgtgggct
      841 gccgcatgat aaacaatagc aggtttgtac tgctcaaaga cttgcaacaa acgatcatag
      901 tcttgaatgt ccgcaatcac aggtacataa tcaatccctt ggaacttacg aatcaattca
      961 tgataaacaa ggtagattga gttttcccca tgaccgagca agacaatgcg ttcaggattg
     1021 aagcgactaa cttgacgaca gatttcagaa ccgattgaac ctccagctcc tgtgactaag
     1081 atggtcttac ctgtcagttc tgcacccaga cgcgattcgt caagacggat ttcctgacga
     1141 cccaaaaggt ccgtaatatc aattttttgg aagccagtac ctgcttggtg aaggccctga
     1201 acaacagttt caaccttagg catcttgtaa catttgacac ccagcttatt acacatctgc
     1261 aagatacgct catattctga cggatccagc gacggaatcg caacgatgac acgctcgatt
     1321 tgatggcgtt tggctaattc aggcagattg tcataagagc ccaaaacagg aataccacca
     1381 agtttttgac cctttttctt agaatcctta tccaaaatac cgaccagttc taattcactg
     1441 gttggatgtt ggtaactatc cataaagaga gccccaccat caccggcacc aatcaagaag
     1501 gtccgacggt gttctccatc accactacct tttttgcgtc tggagtagat taactgccaa
     1561 gtaatccgtg gcaataaaat caagaaggta ctcaacaaga taaagagaat gataaaacgg
     1621 atggagaaga gtggcaagaa ggcataacag atactatatg acaagacact gctagcagtc
     1681 acaccaaaaa agattttcat gaaatccgta atcttgctgt aacgactaat actcgcgctc
     1741 aagccccaaa aatcaaccat caattgatag aacaggaagg ccaaactcgt atagataatg
     1801 tagtcaacag gtgctggatt aatcagccca taaaataaaa tataagatac aatgatggaa
     1861 accaccatac tgaaaatatc aaatattccc cagaaaacct gcttttgttg tttatttaaa
     1921 atttccacca gatcaatcac ataatctgtg agttttttat tcatagaaat ttactcctcc
     1981 ttaaaatagt tctactatat tgttattata gcacatttta atttatttgg cttatttttc
     2041 caaataaatt actgttccct tgtactcctt cttccattta aatcctctaa ttaaacctac
     2101 aatcgtccca aggccataag caaagtgaat ggaaaataaa agaaagggca tcacaattag
     2161 aaatccattt ttatgtttta ataaagtcag caaagtgagt aatgacagta atgataaaag
     2221 tagaaaatag gcacctaata gtaaagttat gaatacgaat gtgatcggta acaatgctag
     2281 actaaacaca agactcaaaa caaataaaca aggaacataa tgaaataatg ataaacactt
     2341 aaactgaaca tgacttgtca agccaatcca caaaccattt gaatactttt gatgcagcat
     2401 tttcttgaat gttggtcgaa tatactgata agatagaata cttgggctat agcggatttt
     2461 ataaccatgt tctcgaattc tataatgaat atcattatct tcagttcggc caagttgctc
     2521 atttactaaa ccaaccttct ggaaaacctc tcgtttatac atcccatgaa aaatagaaga
     2581 aacatatcta tcctcggaac tatttcgata attggcaatg ctactgccaa acatattttc
     2641 ctcaacaaga tgcaaggtct ctgcccattt tccttttcct ttgacaatcg tcggtctagg
     2701 ccctccacag acaaattcac cttgttgaat aacagccaca ttgttcatta caaaactctc
     2761 agtaactttt gaatgagcat caatttttaa aataaggccc cctacagaat gcttaactcc
     2821 caggttaaaa ccactagctt gatttttctt aggattgtta tacaatctaa ttgagttaaa
     2881 ctctgcatct tcttttataa attgctgaat gatagctgtg gtcccatctg tggacatagc
     2941 atttataaat agaatttcaa tattctcttt aggataggtt tgatttttta agtcttcaat
     3001 tagaccaggc agatattttt cttcattgta tgcactgatt acaatggata cgagaagact
     3061 ttcaacattt tgagtcacta ctactctcct gaattttccc ttccaccatt ctatcataat
     3121 tttcaagact tttccatttt tatcttgaaa gccagaaacc ttacttgaca ttatagtgaa
     3181 aaagccttaa aataagctgt tattaaaatc agctagaaga gatattatat gaaaaagcaa
     3241 tcactctttt ttgttccagg tattatcctg attggtgttt ccttgcgaac tccttttact
     3301 gttttaccca ttattttggg aaatatttcg caaggtctgg aggtagaagt tagttcgctt
     3361 ggtgtcttga ccagcctgcc tctccttatg tttaccctct tttcaccatt ttctacccga
     3421 ctggctcaga aaatcggctt ggagcatctc ttcacctaca gcctcttctt cttgaccatc
     3481 ggctcactta ttcgactaat caatctgccc ctgctctatc taggaacctt gatggttggg
     3541 gcaagtgtcg cagtcatcaa tgtcctgctt cctagtctta tccaagccaa tcaaccaaag
     3601 aaaattggtt ttctgaccac cttatatgta acgtctatgg ggattgcaac ggctctggct
     3661 tcctatctag ctgtgcccat tacacaagcc agttcttgga aaggacttat cctcctcctc
     3721 acgttactct gtctagcaac ttttttggtc tggctcccaa atcaccgcta taatcataga
     3781 ctagctccac aaaccaaaca aaaaagtcaa ataaaggtca tgcgtaataa acaggtttgg
     3841 gcaattatta tcttttcagg ttttcaatcc ttgatctttt acaccgccat gacctggtta
     3901 cctaccatgt ctatccatgc aggtctatcc agtcacgaag ctggcttgct gacttctatc
     3961 ttatctctga ttagcattcc tttttcaatg accatcccaa gcctgacaac cagtttatct
     4021 actcgcaacc gtcagctcat gctcactctg gtttcactag ctggtgtggt cggcatttcc
     4081 atgctctttt tcccaatcaa taatttcatt tactggcttg ccatccatct cctcatcgga
     4141 accgcaacca gcgccctctt cccttatctc atggtcaact tttcactcaa gacaagcgcc
     4201 cctgaaaaga cagcccaatt gtccggccta tctcaaacag gaggctatat cctagcagcc
     4261 tttgggccaa ccctctttgg ttacagtttt gacctattcc actcttgggt accagctgta
     4321 gctgccctct tgctcatcga tatcctgatg actgtggccc tctttacagt ggacagagcg
     4381 gataagatcc tttaaacttc tagttttagc tagaagtttt ttatatataa aaattttaca
     4441 catttctctt gacagggctt tcttttagat gtacaatgta tgtgtagaaa aattatatat
     4501 aaaaatctta cacattagaa aaggaggttt cccatgtact ttccaacatc ctctgccttg
     4561 attgaatttc tcatcttggc tgtactggag cagggtgatt cttatggtta tgagattagc
     4621 caaaccatta agctgatcgc taatatcaaa gaatccacac tctatcccat tctcaaaaaa
     4681 ttggaaggca atagctttct gacaacctat tctagagagt tccaaggtcg catgcgcaaa
     4741 tactactcct tgacaaacgg tggtatagag cagctcttga ccctaaaaga tgaatggaca
     4801 ctctatacag acaccatcaa tggcatcata gaagggagta tccgccatga caagaactga
     4861 atacctgact cagctagaac tctatctcaa gaaactacct gaagctgacc gtatcgaagc
     4921 catggactat ttcagagagc tctttgacga tgctggagtc gaaggagaag aagaactcat
     4981 cgctagtttg ggaactccca aagaagtggc ccacgaagtt ctatccaatc ttctcgataa
     5041 aaaaatcaat gaagcacccg ctcaaaaaaa taaccgacaa attttacata tcgccttgtt
     5101 agccctcctt gcagcaccta tcggcattcc tctgggaatc gccatcctcg tgaccctgtt
     5161 cgcaatcctt gtagccgctt tgactgtcat tctggctttc tttgcagttt ccatactggg
     5221 tatcatcggc ggattcctat ttttagttga aagtttcact gtcctcgccc aagccaaatc
     5281 agcctttatc ttgatttttg gttctggttt actggctatc ggtgcttctt cgctagtttt
     5341 acttggcatt tcctatgtag ctcgcttctt cggtctactc attgttcgtc tggtacaatt
     5401 tgttcttaaa aaaggaaaga gaggtaatca gcatgcgtaa atggacaaaa ggatttctca
     5461 tctttggtgt ggtgactacc gttatcggct ttatcctgct ttttgtaggt atccaatctg
     5521 acgggattaa gagcctactt tccatgtcca aagaacctgt ctatgatagc cgtacggaaa
     5581 agctaacctt tggcaaggaa gtcgaaaacc tagaaattac tctccaccaa cacacgctca
     5641 ccatcacaga ctctttcgat gatcaaatcc acatttctta ccatccatct ctttctgctc
     5701 accatgatct tatcaccaat cagaacgata gaactctgag tctcactgat aagaaactgt
     5761 ctgaaactcc gtttctctct tctggaattg gtgggattct tcatatcgca agtagctact
     5821 ctagtcgttt tgaagaagtt attctccgac taccaaaagg gagaactcta aaagggatca
     5881 acatctcagc caatcgcgga caaaccacca tcataaatgc tagccttgaa aatgcgaccc
     5941 tcaatacaaa cagctatatc ctccgaattg aaggaagtcg tatcaaaaac agtaaactca
     6001 caacgcccaa tatcgttaat atctttgata cagttcttac agatagtcag ctagagtcaa
     6061 cagagaatca cttccacgct gaaaatatcc aagtccatgg taaggttgaa ctgactgcca
     6121 aagattatct cagaatcatc ctagaccaga aagaaagcca acgaattaac tgggacatct
     6181 caagcaacta tggttctatc ttccaattca caagagaaaa gcctgaatca agaggtacgg
     6241 aattaagcaa cccttacaaa actgaaaaaa ccgatgtcaa ggatcaactc attgcgagat
     6301 ctgatgataa tattgatcta atatccacac caagcagacg ttgacaaaaa cgcttacatc
     6361 tgctactatg gaagcatatt tatctaacta aaaaggaggt tctaccatga aacaagaatg
     6421 gtttgaaagt aatgattttg taaaaacaac aagcaagaac aagcctgaag agcaagctca
     6481 agaggttgca gacaaggctg aagaaacgat agccgatctc gatacaccaa ttggaaaaaa
     6541 tactcaagta gaggaggaag tctctcaagc tgaagtcgaa ctggaaagcc agcaagaaga
     6601 gaaaattgaa actcctgaag gcggtgaagc gagaacagaa atagaagaaa agaaggcatc
     6661 taattctact gaagaagagc cagacctttc taaagaaaca gaaaaagtca ctatagctga
     6721 agagagccaa gaagctcttc ctcagcaaaa agcaaccacg aaagagccac ttcttatcag
     6781 taaatcttta gaaagtcctt atatccccga ccaagctcca aaatctaggg ataaatggaa
     6841 agagcaagtg cttgattttt ggtcttggct agtggaagcg atcaaatctc ctacaagtaa
     6901 gttggaaaca agtatcacac acagttacac agcctttctc ttgctcattc tgttttctgc
     6961 atcttccttt ttctttagta tctatcacat caaacatgct tactatggac atatagcaag
     7021 cattaacagt cgcttccctg agcagctagc tcctttaact cttttttcta tcatctctat
     7081 cctagtagcg acaacactct tcttcttttc attcctcttg ggtagtttcg ttgtgagacg
     7141 atttatccac caggaaaagg actggacgct agacaaggtt ctccaacaat atagtcaact
     7201 cttggcaatt ccaatcttcc tcactgctat tgctagtttc tttgctttct ttgatagcct
     7261 acgatttacg gccctcttgt gtgtgattag cattggaatc attctgcttg ccagtctcca
     7321 tatcattaca agaccaagtc aagcaagtga aaccgactcc ttctatcaat tattcttgtc
     7381 tgtccttgtg aacggagtta ttatcctcct cttctttgta gctgaagtgg cactgattgg
     7441 agattatctt cgtatcttgg cctttcttta aaattcaatc ctgtcatcct tggcaggatt
     7501 ttttctatgt caaaaaagca agtcgattga cctgcttctg ctttactttt cttgtgctaa
     7561 tgttttaaaa tctttgtcta atatgggttg cacttctatt tgattggcgt ctagttggtg
     7621 gtaaaaagat gggggtaata cctctaagaa ttttgcatag tccaagcgag caatggcttc
     7681 atagtcttct ggtttagagc catctcctgt gatttgaacc aagtcaatct cccactgcac
     7741 ttccttatcc accaattgct caatataggc tgcaggaaca aacggttctc tcggtaaaac
     7801 tgtcttgact ccttgagcta gatggtaaac gccatgcact tttccttctc catcctgata
     7861 gaaggaaact ctcgcaaagt aggcatctgt ctcttgaacc gcacgcacac gcgcttcaaa
     7921 ctgagccaaa ccatctgcct ctaaaaagct tttcaagttc tcacgactaa agaaaatagg
     7981 attaaaaatt ccttggcaaa tcgtaaaacg agctacaggg tctgctagat aggcttgaat
     8041 acttgcttgg aaataagctt caaaatcaaa accatctagc ccttcaatat cagtccctgt
     8101 ataagcaagc aaagcatcta aaaatgactt gataatctgc cagtctgtct ctgttagtag
     8161 gtcaggaaga tcgacacggt aagccttttg accatcagca taactgacct taaaaagaaa
     8221 ttgtgattgc cccactatcg cacactcaat atactcgaga cgattaagag gctgacggag
     8281 atagactaca tcatagatat gtgactccaa accgtctagc aatgccaaga tcgacttggc
     8341 attcaaaatc tcttgttctc ctaaaatgct ctgtttattt ggaataaaaa atgttttcgc
     8401 cataactgga ctcctttgaa atcgcttaca tacagtatac cttatttttc tgaaagatac
     8461 agaaacaaac tttagatttt tgagtacaaa gcatagaaaa acagcccctg aggactgttt
     8521 gatcttatac agtagctgct tgatccaagc tttcaccgat agcggctagg cgctcgatca
     8581 cttcagcttg tgtcaattca ttttttgaaa catagcggtt acgtgggtga acacggcact
     8641 cgtgtgagca tccacgaagg tacttgtctt cattttcttc tgatgtcaag atacgacggt
     8701 tacagaaggg atttccacag ttgacataac gttcacatgg tgttccatca aaccagtctt
     8761 tccctacgat ggttgggttg acatggttga catcaacggc gatacgctcg tcaaatacgt
     8821 acatttttcc atcccaaagc tcaccttgaa cttctgggtc tttaccgtaa gttgcgattc
     8881 ctccgtgcaa ttggccgaca tctttgtagc cttcacggac catccagcct gagaatttct
     8941 cacagcgaac gccacctgta cagtaaacca cgacacgctt gtccatgaat ttttccttgt
     9001 tatcacggac ccattgtggt aactcacgga agttgcgaat atctgggcga atagctccac
     9061 ggaaatgtcc taggtcgtac tcataatcgt tacgtgtgtc aaggacaacg gtatctttat
     9121 caagaagcgc ttctttgaac tcttttggag acaagtaagc acctgttgtt tcaagtgggt
     9181 tgatgtcatt gtcaaagtcg ttgtcttcca aaccaaggtg gacaatttct ttcttgtagc
     9241 gaacaaacat cttcttgaag gcttgttcat tttcttcgtc aatcttgaac cagagttctt
     9301 ccattcctgg aaggctgtga acgtagtcca tgtatttttg agttgtttca tagtcacctg
     9361 aaactgttcc gttaattccc tcgtcagcga ctaggatacg gcctttaaga ccgattgatt
     9421 tacagaaagc caagtggtct gcagcaaatt gctctgcatt ttcaattgga gtataaaggt
     9481 agtaaagtaa gacacgaata tcttttgcca taagatttgt tctcttttct attcttaaat
     9541 tttctaaatt ttttatttat ccatactagt atacctgctt gagaaaacga atgcaattta
     9601 tttgactgga aattgtagag gattctcgaa atttcctgaa tggttaaaat aaggacaaga
     9661 gaaaacatgg atatctatat ccttgtgcca aaaaaaccac tgccctcccc agaccaacct
     9721 gaggaaagca gtgattctta ttttaggagt taggaatgaa tacacgaaat caatttagct
     9781 gattattttt tgtttttcaa gaattcatcg tattgttttt gcatttcgtt caatactttt
     9841 tcgtaggcac cttcagattt caatttttcc atcaattctg gaatcgcttt atctgggtct
     9901 acagtaccag tgttgatagc tgtatcaaat tgttgcattg tgttagcaat agctgagatt
     9961 tcagatttca cattgtcagt attgaagata aatccaagcg ctggagattc tttagcttct
    10021 gccaattctt tcttagaatt ttcgatttgt tggtctgtaa cgttttcgtt gatgtaaagg
    10081 atccagttgt taccagtgtt ccatccaccc atgtgagtgt ttcctttgta gccatcaaga
    10141 acgcgaacac ggttttcttt accttcaatt ttttcccagt tcttgccttc tggaccgtaa
    10201 acaagaccgt tcaaaagttc tgggttcgta ttcaagaggt tcaagatttc cattgatttt
    10261 tctttgttct tagagttgtt tgagatgaca aagttagcaa cttgtgttgt ttggtttttc
    10321 ttgatgaagt tagtgattgg tttgatttgg atatctttgt tggcaacacg tgaaagcaag
    10381 ctgttaccgt agtcagctgg tcctactgtt tcttcacgaa cgaaccaagt atcttgttga
    10441 aggtcaaatg aagtatcgct tgttgcgacg tcttttggaa tgtagccagc ttcatagaat
    10501 ttgtgaagag tcttcaagtg ttctttgaaa cgaggcactt cgtaacggtt tacaacttta
    10561 gtagtatcgc cttcaaggtc aataacgaat ggaagcccgt ttgctactgg gtagtcaaaa
    10621 ttatcagatg ggatgaaaac tttaccaata gcaaatggta ctacgtctgg agctttttct
    10681 ttgatttgtt tcaagactgg ctcaagagtt tcgtaagaag taacacctga aatatcgata
    10741 ccatatttgg caaggagagt tccgttgaag gcaaagtttt gagatgatgc tacgttggct
    10801 gcaactggta cagcataaat cttaccattt acagtattac ccttgatata agctggatca
    10861 agtgccttgt aaaggtcttt accttctttt ttgtacaatt ctgtcaagtc agcataagca
    10921 cctttttgag catttacaat atagttatct gcaaaggcga tatcatagtt ttcaccagat
    10981 gatgtgataa ctgacatttt cttaccatag tcaccccagc caaggtattg gatatccaat
    11041 ttggcaccaa ctttttcttc gatgattttg ttggcatttg ctaacaattc atccaagtta
    11101 tctggtttgt caccgatttg gtacattttg ataacaggtt tgtcacctga atcagcagct
    11161 tttttgctgt tacctgtcaa atttccacaa gcagcaagac ctgcagccag agcgactaca
    11221 ctagcagatg caaaagcata ttttttccag tttttcatga taaaaactcc tttttttatt
    11281 tttaaactta taaacaatgt aatgatctta acttcacaca agggaagttg ggaactgaga
    11341 aatgttcttt ttcaataagc actattcttt cacaccaccg atagtcaaac cttttacaaa
    11401 gtagcgttgg aagaatggat acaaaatcgc gattggaagg gttgctacca caaccatggc
    11461 catacgacct gtttctttcg gtagagcaac tcccagttga ccagtcaagc cgaccgcttt
    11521 ggcaatgtag tccatatttt gttggatttg catgagcaaa tattgcaatg gatacaagtt
    11581 atcactcttg atgtaaagaa gggcgttgaa ccagtcattc cagaaaccaa gagctgtcaa
    11641 aagcgtgatg gttgcgatac ctggtagtga caatggcaaa cagatttgga agaaaatccg
    11701 agcctcactg gcaccatcga tacgagccga ttcgagaatg gcttctggaa tggtcttctt
    11761 gaaaaaagaa cgcatcaaga tgatattgaa tggtgagaga agcattggaa caatcaaggc
    11821 ccaaacagtg tcaccaagct gaagtacacg ggtcaccatg atataacctg gtaccaaacc
    11881 agcgttgaac aacatactga gaaggacgaa gatggtaaaa aatctgcgat acttaaaggt
    11941 tgtccgtgaa atagcgtagg cataggttgt tgtgataaag acatttgtca atgtcccaac
    12001 tacggttaca aagacagaga tgaagagggc ttgtaggatt ttatccttaa actgtgccaa
    12061 aaactcaaaa ccgtctaagc caaattggga tgggaagaag ctatagccgt attggaggag
    12121 gcttttctcg tctgtcactg aaataatgat aacgaataca aaaggtagga tacaagagag
    12181 ggcaatcaaa cccgaaatga tactgaagaa gatatctgct ttcttactga aggagtgaat
    12241 gccgacatta tcaatttttt cttttttaat tttctttttt gccatattct cctcctttct
    12301 agaacaaagc tgagtttgga tcgactcgtc ttgcaagcaa gtttgatagg ataaccagaa
    12361 tcaaaccaac aacggattgg taaagaccgg ctgctgcagc cataccgata tctgctgtct
    12421 gagtcaaacc attaaagaca tatacgtcca aaacgttggt tacattgtaa agctgaccag
    12481 cattgtgtgg gatttgatag aagagaccga agtctgcgcg gaagatattt ccgactgcaa
    12541 ggatggtcaa tacagttaca agcggagtca actgaggaat ggttacgttg cgaatacgtt
    12601 gccacttgct agctccgtcc actgtcgctg cttcgtagta ggttggatca attcccatga
    12661 tcgtcgcata gtacatgaca ctgctatatc caaagccttt ccaaatacct aggaaaagta
    12721 ggagataggg ccagatgccc aggtcagcgt agaaattgac ttctttgaga ccaagacttt
    12781 ccaatagatg attgaacacc cctttatcaa tatttaggaa ggcatctgta aagaaactga
    12841 tgataaccca agacaagaag taagggaaca acatagaagt ttgaaaaatc ttcaccattc
    12901 tcttagaacg gagctcgctg aggataatgg caatccctac agatacaact aaacctagaa
    12961 agataaagcc aagattgtag aggacagtat ttcgtgtgat aataaaggcg tctcttgaac
    13021 taaataagaa tctgaaatta tcgagtccga cccatttact atttatgata ctatctatga
    13081 aaccattact ggtcatgtgg tagtctttga aggcaaccac gttcccaaat actggaatgt
    13141 aaaagaatag aatcaaccag agtgcccctg gcaaaaccat caagagaaag atccagttgt
    13201 ctctcaatgt ttttgaaaac tttttcataa tttcctccct ttttattttg atatccatct
    13261 aaaaattctt ttttagactt ttgataacga ttacattatt agtatactcc tatttgcagg
    13321 ttaggttaaa ctcctaatta tagaaaaaac tccacaaatt atgtagcaga tttaaaactt
    13381 tatcaccact atcaaacaaa tgtcctaaat caattgttta ttttatctct attagcccag
    13441 tgatggcgtc actctgttat aagcatccaa caacggggta tactgaaaaa tctccagact
    13501 agggaactca gcgatagttc ctaatctgga gatttttaat atgttattag gcgtttgctt
    13561 tcaacttagc aataacctct ttaagattat caatcaactc tgcttcagta tgctcagagc
    13621 ctttttcatc tgccaagaac aaaactgctt tttgaagttc ttcttgagag ttttcaagga
    13681 catccttatc tgctgtttct ttgcccgctg tttgtcctcc atccttagtt gctgactggc
    13741 tttccttaat ggactctagg gaagcaatgg catctttgac tgtttgcaag atatcacgta
    13801 aaccttgctc tgtcaaacta tcatctgcaa aagctttctt agcctctgcc aaaaccagac
    13861 gtgctgaatc tgtggtagga ttcgatacac ctgtcaatga tctcaaaaga ttttctaaag
    13921 tttgagtctg cttactaata ctagactaaa atcaaaaagc attatataac agtgatatga
    13981 aatcaactaa agaagaaatc caaac
//
LOCUS       DAVBCW010000050        13836 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVBCW010000050 DAVBCW010000000
VERSION     DAVBCW010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13836
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.050"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            182..658
                     /locus_tag="VQ514_001236"
     CDS             182..658
                     /locus_tag="VQ514_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2499992.1"
                     /translation="MKLSLYSEVEHYHFLSSYSLNDITYTDTPKNIIEKSKKDKYRYP
                     IVILNKDHKVIGFFCLHLNTGPKEYGYYQNDYALIRGFSIHDNFRNQGYGSNALNEIF
                     EFIDSTLKLEINHIILAVNEKNILAQKAYRNSGFSVVKKDLEGKRGKLIIMEKSRN"
     gene            complement(1332..1724)
                     /locus_tag="VQ514_001237"
     CDS             complement(1332..1724)
                     /locus_tag="VQ514_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HET2499993.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLTRQIAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTDKSQYEFLFPSVK
                     IKEEKTDKKEEVATDSSEKAEKKIRREA"
     gene            complement(1702..2163)
                     /locus_tag="VQ514_001238"
     CDS             complement(1702..2163)
                     /locus_tag="VQ514_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HET2499994.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLI
                     SEVRYQQQSEQKEWLLFVGQLEAELDRSQFEKVEGNRLYMKQDDKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES"
     gene            complement(2126..2386)
                     /locus_tag="VQ514_001239"
     CDS             complement(2126..2386)
                     /locus_tag="VQ514_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2499995.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            complement(2391..2819)
                     /locus_tag="VQ514_001240"
     CDS             complement(2391..2819)
                     /locus_tag="VQ514_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HET2499996.1"
                     /translation="MEGQIVKSMIKAFTMLESLLVLGLVSILALGLSGSVQSTFAAVE
                     EQIFFMEFEELYRETQKRSVASQQKTSLNLDGQMISNGSQKLTVPKGIQAPSGQSITF
                     DRAGGNSSLAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            complement(2788..3114)
                     /locus_tag="VQ514_001241"
     CDS             complement(2788..3114)
                     /locus_tag="VQ514_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET2499997.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLDKNEDATLSKLQGDGRITEEQVKAYNEYYTKNGGA
                     NRKVND"
     gene            complement(3116..4132)
                     /locus_tag="VQ514_001242"
     CDS             complement(3116..4132)
                     /locus_tag="VQ514_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET2499998.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLDFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            complement(4080..5021)
                     /locus_tag="VQ514_001243"
     CDS             complement(4080..5021)
                     /locus_tag="VQ514_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HET2499999.1"
                     /translation="MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKMASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAN
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            complement(5097..5462)
                     /locus_tag="VQ514_001244"
     CDS             complement(5097..5462)
                     /locus_tag="VQ514_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HET2500000.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            complement(5613..6671)
                     /locus_tag="VQ514_001245"
     CDS             complement(5613..6671)
                     /locus_tag="VQ514_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HET2500001.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPKKAIKEIYDLTDGRGVDVAIEAVGIPETFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            complement(6834..7985)
                     /gene="nagA"
                     /locus_tag="VQ514_001246"
     CDS             complement(6834..7985)
                     /gene="nagA"
                     /locus_tag="VQ514_001246"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HET2500002.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            complement(8138..9955)
                     /locus_tag="VQ514_001247"
     CDS             complement(8138..9955)
                     /locus_tag="VQ514_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HET2500003.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMIFLLSATAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPILQTLRPLPYIHAILAAGTGILTFIVCMVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            complement(10056..11198)
                     /gene="tgt"
                     /locus_tag="VQ514_001248"
     CDS             complement(10056..11198)
                     /gene="tgt"
                     /locus_tag="VQ514_001248"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HET2500004.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMLLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFRKYFVEKYGYNKSGRNF"
     gene            11328..12185
                     /locus_tag="VQ514_001249"
     CDS             11328..12185
                     /locus_tag="VQ514_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HET2500005.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSGFLNP
                     LSRIHGTVLEQPFFSILGQILQTYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            complement(12213..12857)
                     /gene="pcp"
                     /locus_tag="VQ514_001250"
     CDS             complement(12213..12857)
                     /gene="pcp"
                     /locus_tag="VQ514_001250"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HET2500006.1"
                     /translation="MKVLVTGFEPFGGEKGNPTLEAIKGLPAEIHGAEVRWLEVPTVF
                     HKSAQVLEEEMNRYQPDFVLCIGQAGGRTSVTLERVAINQDDARISDNEDNQPIDRPI
                     RPDGASAYFSSLPIKAMVQAIKKEGLPASVSNTAGTFVCSHLMYQALYLVEKKFPYVK
                     AGFMHIPYMMEQVVNRPTTPTMSLVDIRRGIEAAIGAMIEHGDQELKLVGGETH"
     gene            complement(12964..13332)
                     /locus_tag="VQ514_001251"
     CDS             complement(12964..13332)
                     /locus_tag="VQ514_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HET2500007.1"
                     /translation="MSIITIILTTIVALEHFYIFYLESIATRSDATSRVFNMEKEELA
                     HPSVSSLFKNQGIYKALLGVFLWYGIYFSQNLEIVTIFVLFVIGAATYGSLTADKKII
                     LKQGGSAILALISILLFKYT"
     gene            complement(13343..13768)
                     /locus_tag="VQ514_001252"
     CDS             complement(13343..13768)
                     /locus_tag="VQ514_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HET2500008.1"
                     /translation="MIEIQDLLYQLRLSEQASTQLFEKRLGISLTRYQILLFLLEHSP
                     CNQMAVQERLKIDQAALTRHFKILETEGLVERHRNPENQREVLVEAAKYAKEQLVVNP
                     PLQHIKVKEEMESILTEFERTELSRLLNKLVLGIENIEI"
BASE COUNT         4108 a         3102 c         2427 g         4199 t
ORIGIN      
        1 tggtattatc aatcatgaac taggattcta gttttgcagg aagtctaata atgaaaataa
       61 aggactaaag caataactct aaatacatct gatttttata ggatatttca taaaaaaaat
      121 ctacatacct agagtcctat ttagttatgt atcttacata aagagaaagg aattaattat
      181 catgaaatta tctttgtata gtgaagttga acattaccat tttttaagtt cttattcttt
      241 aaatgatatt acatatactg ataccccaaa aaatattatt gaaaagtcta aaaaagataa
      301 gtatagatac ccaatagtaa tacttaataa agaccataaa gtaattggat ttttttgcct
      361 acacctaaac actggtccta aagagtatgg ctattatcaa aatgattatg cgcttattag
      421 aggattttct attcatgata atttcagaaa tcaaggatat ggttctaatg ctctgaatga
      481 aatatttgaa ttcatagact caactttaaa attagaaatt aaccatatta tattggctgt
      541 aaatgaaaaa aatatattgg ctcaaaaagc atatagaaat tccggttttt ctgttgtgaa
      601 aaaagattta gaaggaaaaa gggggaaatt aattattatg gagaaaagca gaaactagta
      661 atgatactag tttctgcttt tctctaagtc tgctataaat ttataacatg ttgaaataag
      721 atgtgaataa attgattgca gaattcaaac caatttctaa caatgtttta gaaaactcgg
      781 cggactattc cagattcaat ccactatatt ctatcttatt tgcatatttt gagagaaata
      841 atctatatac taaactttta tgtttataat tcattccttt cgtttcactc aaggcacaag
      901 acagaatgaa aaagtggtgt gctctttatt ttgttttata ataatagtga gaaaacctat
      961 cactactaca aatcacgggg aggtgaataa gtgagtggta tagccactac ctcgcatttt
     1021 tataggtgtc attctttcca tcaagcacaa gaactgtgac taggagcacg taaacttata
     1081 attgaataag cgactcgttt gtgaaaggaa agaatagtca agggataggc agactcaaat
     1141 ttggtgaagg gaaacccctc atgttaaaag ccaaacttca attttaaaaa ttttaaaatt
     1201 tgtttcgtat tcactactcc ttgcctttat attggattga ttaagagaac ctttcgcgtt
     1261 tctagaaact acagtggact attctggatt caacgcatta tagttgaatt gactaggaat
     1321 tctctttctt ttcaggcttc tcttctgatt tttttctccg ctttttcgct tgaatcagtc
     1381 gctacctctt cctttttatc cgttttctct tctttgattt tgactgaagg aaacagaaac
     1441 tcatattgac tcttatccgt acgaacagtc gtcaccaagc ttgttttctt gttttgatag
     1501 ctcacctgac ctagattaaa aacctgttcc ccactttctt gctcggcttt atccttggtt
     1561 cgtttagcca tggcaaaagc aaccaatttt tctttattta aagcatagtc ttgatagtgg
     1621 gcgatttgtc gggtcaaata aaattgcaac aaaagactaa agatggctgc tatggtgact
     1681 gcgtagagga gaacacctgc cttaactttc ttctttttcc acacgataga tgaactccct
     1741 ttctaagcct ttttgaaact ggaaacgaaa atgaaccagt tgattgtcct ctgtaatctg
     1801 agctgatttg aggccataaa ccataggctg atacccccgt ccactagcat cggttttacg
     1861 aaaatcgtct gactttgact taccgatggc gatgtccttg tcatcttgct tcatgtatag
     1921 gcgattgcct tctacttttt cgaactgcga acggtctaat tctgcctcaa gttggcccac
     1981 aaacaagagc cactcctttt gctcgctttg ttgctggtag cgaacttctg aaatgaggag
     2041 ctgactcata gcttgaaaaa ggagtaatcc cccactgatg acaatgaggg caagcaggga
     2101 ttctaacaag gtaaaagcct tgaccttatg gctctttgat tgccaacaac tgttctgaac
     2161 catggtagac ctccaatccc ttttcactag aaaatacctg aatctcaact ccgttgatgc
     2221 ttacctgatt ttgccccgtc tgcaaggcca tcttagctac cctcaagact tcttcctttt
     2281 gcaagatttt tgcttcctct tgcctatttt tttgaatttg tcccaaaagg agggtcgcaa
     2341 tgctggcaaa gatagctaga gcgactactg cttccagtaa aatcactgcc ctaatttttt
     2401 gtttccttaa tgcgtttaat ttttccattt cctagatata attgatagcg aatcgctcct
     2461 ttactggtct gaaattcaac cttagccagg gacgaattgc ccccagctcg gtcaaatgta
     2521 atactttggc ctgatggtgc ctgaattcct ttaggaactg tcaacttttg actgccattg
     2581 ctaatcatct gcccatccaa gttcagacta gtcttttgtt gactagctac actgcgtttt
     2641 tgggtttccc gatagagttc ttcaaactcc ataaagaaaa tctgttcctc taccgctgca
     2701 aaagtggact ggacagagcc ggacaagccc aaggcaagga tactcacaag tcccaaaacc
     2761 aagagacttt ccagcatggt aaaggcctta atcattgact ttacgatttg cccctccatt
     2821 tttagtatag tattcattat aagctttaac ctgttcttcc gtgatgcgcc catctccttg
     2881 taacttgctc agagtagcat cttcattttt atccaagcta taaagttctg cctggctttc
     2941 caccacctta acaacagctg cttttccttt gtcattgact gcttcttttt gcttggtcag
     3001 attaggtaca aagagcaaga aaagcacgct gataatcagc aagaccacca acatctccac
     3061 caatgtaaaa gctttaacct tagctttttt caagaatgtc atcatttttt tcattttaaa
     3121 aatttacctc catattttga tacatgggca tgagcattgc cgcataaagt aaaacgataa
     3181 tcagtgccac aaagataaaa accagtggct gcaccaaatt catggtgcgg ttgactcggg
     3241 taaaaaaggc ttcccaagtt ttttcagcat agatttccaa ctcactaccc agcttggact
     3301 taacttcccc atactctatg atgagactca attccttcct aaagaaagga taggttccta
     3361 tcgtctgaga aaattcacgg ccatttttca gggtttgagc cagatcttga ccgatttctt
     3421 taaagagctg ggaaccttgt tcctgcatca tttgaaaaat ctgcgtcagc tccattccct
     3481 gtgaaatcat attcccccat tcacgtgcat aataggctgt caagtaggtc tgcacaaaga
     3541 ttccaataaa gggaaggcgt gctaagatag aaaagacact catcttagaa cttcttttat
     3601 aaaaagttag tgctaaaagg gcaagcacgg aaacaagccc taccatgcct agaaaaattt
     3661 gtggcagatt accgataatt cgggtggcaa tattgctact atccagttgt gggagcaggt
     3721 aattccgtag ccccagcata attaagagaa gaaaatccag caaaatcaaa ggataggtcg
     3781 ctacttcaat taactttttc ttgaccttgg ccagattgtc tagatattct tctatctttc
     3841 ccaaactcag gtgaagattg ccatgaactt cagctagaga taactgagtg acaatggtac
     3901 ttgaacatcc caaactttcc atcatttctg agaatgattt cccctgagac aagcctgtac
     3961 gcatctgggt cacacactgc ttgtccaaca aggaactcct atctaaaaag gagatagtct
     4021 ccaccagatg aaaaccgcta gaaaagagat tgttaaatag ggtgatgata tttttttgct
     4081 tagctgtagc taattttttc cgtctcagcc tgaagacttg tgatatgtcc atctttaaga
     4141 agttggtcaa tttgctcatt ccacttggct gcttggtgtt cttgataatc tctgtttgca
     4201 aagtcaacga ttcctcctcc cccgattaat ctctggtagc agactccttg cagaacaact
     4261 gccaattctt cttcactcac acccaactcc agcagacgct cataaacacc tcggatactc
     4321 ttggcgtgaa tggttgaaaa gactgtcgca cctgtcaaac tagctctgac cactgcacgc
     4381 gccgtctcgc tgtcacgaat ttctccgata atcaagagat ctggtcgatg acgcaaggaa
     4441 agtttgatta gattttcata ggttaggccg attgcttcgt tcaactgcaa ctgaagcatg
     4501 tcgtcctgct tgatttcgac aggatcttcg atggacataa cttgctgtcc tttaaagagt
     4561 gacttggaca attcatgcat caaggtcgtc ttaccactcc caaccgggcc agcaaaaaga
     4621 tagagtcccc gttgcctgta ctgcttgcct aattcttcaa tatcctgaaa ccaaaaatgc
     4681 aagtcctgct cctcatcgtg caacaaacgg ataaccaaac tctcatgccc ccgataatcg
     4741 cctacagtag ataaacgtag agacgccatc ttctggtcat aggcataatc acaggaaccc
     4801 agttgactac gtcttttttc tcccacattc atacccgcca caaacttaaa gtgactgata
     4861 acggctgcaa acttttcaaa atcatagcta ccaattttac agcgctcgtc tcctaccctc
     4921 atatgaagct cataggcgtc taacttaggg acaaaataaa tatcctgcgt cccttttttc
     4981 cgagctgaac gaatgatttc ttgtgcaatt tcttgaacca tacttacctc ctcacctata
     5041 ctattcgcaa agcgttagaa aaataaaaaa agcaactcca aaaaagttac ttttctctat
     5101 tttaatttca tacggcaaga acgattcctt tcctgactgg tttgtttttc atagcctgag
     5161 cgtagttttt cgtctgggat aacctgctca tcctgcaaaa gagccaaaga atggtattgt
     5221 tgtaaatact catcacattc atcacaccag cgttggtctt ccgggtccca aaaaatggtc
     5281 atcaagtcgc ttctactttt ataaagaggg gattcttgtt ccaatctaaa ccagcaagtt
     5341 ttgtagtatt tgagagcatc ataatactgg tcaaattttc tacttgctac aatatcttct
     5401 tcccaacctt ctaagaacca ccacggttca aaatctccgt acatttctat aacacgatac
     5461 atattcattc attcctttgt accgtatatt ataaccaatt ccgccagaca aggaaagaaa
     5521 tatgctcatt tcttaatttt ttgataaaaa atccagccat ctgatggatg actgaatttc
     5581 tatttgctta tgtacaaaaa tatttttact acttaggctt ctgagatatc gttttcgata
     5641 atgaccttaa tggcatggtg gtctgctgcc ttactgaaga cttcgtaggc tttttcaatt
     5701 tcactgagtt tgaaatagtg agttaccaat ttttccggtt caatcttatg actttcaagt
     5761 gctttcaaca attgtggagt cgtatttgta gataccaaac cagttgttac attgatgttg
     5821 cgaatccaaa gtttatctaa atcgaattca actggtttac catgcacacc acagttggca
     5881 accgttccgt ctacaccgat aatcttttga cagaaatcaa atgtttcagg aatccctaca
     5941 gcttcgatag cgacatccac accacgacca tctgtcaaat cataaatttc tttaatggct
     6001 tttttagggt ctgaagaatt aaccttatga gtcgcaccga atgatagggc agtttccaag
     6061 cggttatcgt ctaggtctac cataatcaat ttagctggtg aatagaattg ggctgttaaa
     6121 agagcagcca atccaactgg acctgaacca ataatggcta cgctgcaacc aggttctact
     6181 ttccctttta agacaccaat ttcatatcca gtaggcagaa tgtctgacag cataaccaaa
     6241 gcttcatctg acaagtcttc tggagtatgg taaagagtat tatctgcatg agggacacgt
     6301 agatattcag cctgcatacc atcaatcaag tgaccgaaaa tccagccccc ttcgtcttca
     6361 cagtgggcat aaattccttt tttacagtag tagcatttac cacaggcaca gacgcaagaa
     6421 atcaagacct tgtcaccttt tttgaagttg gaaactcctt ccccaacttc ttcaacaatc
     6481 ccaatccctt cgtggccaag aatggtacca ctttggcaag taggaacatc ccctttgata
     6541 atatggaggt ctgttccaca aatagtggtt tttacaatac gcacaatagc gtctgttggc
     6601 ttgcgaataa ctggtttgtc tacatcaaca aaagaagcaa gtcctggttt aacataagta
     6661 taggctttca taaagcactc ctccgttttt ttatttgtaa tatttatagt tcaattgtaa
     6721 gcctttttct ttgtttcttc aagctttttt tgtgattttt ttaacttttt atttaccagt
     6781 aaagtaccct gtataaaaaa gcagaagcta gtctctaact tctgccttct ctcttatgct
     6841 tgataacgtt tcacaccatc taggtaggtt gctaccaatt ccaaatcttt atctaatacg
     6901 atgaagtcag cgtcgtagcc ttcacggatt tggccacaga cgtcatcgat gtgaacggat
     6961 ttagctgggt tgaagctggc catcatgact gcttcatgcg gattcgcaat tccccattcg
     7021 accacattct tcaaaccatc tttgagtttg aggatagaac ctgccaagtt acctgtcgat
     7081 ttgaggcgtg cagttccatt tgcaacgaca actgggaatt ctcccaacat ataatctccg
     7141 tcttccaatc ccccagctgt catacagtct gtgataagag cgatgttttc tgttcctttt
     7201 tgtttgataa gaatttcgca agcctttgga tctacgtggt gaccatcaca gatcaactct
     7261 gcataggtat gtggcaattg gtacatggct ccaaccatac ccaattcacg gtgagtcaac
     7321 ccacgcattc cattgtaggc atgcacccaa acactcgctc cagcatcgac tgcttttttg
     7381 gcttcatcaa aagtcgcgtt tgaatgtcca agagcaaccg tcacaccttc gcccgtaact
     7441 gtacgaacaa agtcttccac cccatcacgt tctggtgcaa gggcaatttt attaagcaag
     7501 ccatttgccg ctttttgcca agaatgaaac tcctcaacac ccgggtctct catataagtt
     7561 ggattttgtg cccccttaaa agtttctgtg aaatatggac cttcataata aatcccacga
     7621 atcttagcac ctgttgcttc tttataatgg tttccaagat tttcagtgac tgcaagcaat
     7681 tgctcataag tggctgttaa agttgtgggt aagaaactgg taacaccggt actaagaagt
     7741 ccttcactca tagtatgcaa tgtaccttca atgttgttgt ccatcacatc tacacctgca
     7801 tatccatgaa tatgagtatc cacaagtcct ggggcaatgc tataacctgt atagtcaatc
     7861 acctcagccc cttcaggaat ctgctctaca tgtttcccaa acttgccgtc cacaagttcc
     7921 aagtaaccac ctcgacgaac tccgtgtggg tagaaaaact gatccgcttt aatatagtta
     7981 ggcataatgt taacctcctt aaaagattga ttctacaatt tattatgtca attacattat
     8041 aaacctttct tttttatttg taaatggtat agacctattt tttggagata ttttaaaaga
     8101 gctggatttc tccaactctt ctctttttta atacaagtta ttttgattta accggcttgt
     8161 cttgagctgt ctgcaaagct gtggcaatcg tatctgcata caattttgct cctgcttcga
     8221 tagtgctact ctcactcccg aaatgaacct ggtctgttcc agcccaaatt tctggatgct
     8281 ctttcgcaac ttgattccaa tctgctatcg taatgtaagg tgtcttctct gccaattctc
     8341 tcatataggc agcagccttc tcaacgatgg cataggtctc ttttgtctta tctccctcat
     8401 aaggagtcac caaaatcata tggtgtccct taggaagatt tttcacgata ctgtcccagt
     8461 catccttgta attctcagga ttatttaccc cagtcgcaat gaccaccgtc ttaggtaaaa
     8521 atttattctg gctattattt agcatgattt catttgcggt cttggttgtt acgctgacct
     8581 gcgcgttaat ctgtgctcca ggaagagctg tctgtagtgc tgtatttgcc cttaaagcca
     8641 ctgagtcacc aattaacata gtgccatcag caattcccaa actgtttgca tctgcccgtt
     8701 ctgccatcac cttggtctgg ccaatatttg ttgcagcttg cttcaagcca ttgacagtca
     8761 agtctgtctc aaacgctccc acttgtggtg ccaacaaggt caccatgcag acaatgaagg
     8821 tcaagattcc tgtacctgct gcaagaattg cgtgaatata aggcagggga cgaagagttt
     8881 gtaaaatagg tgtgttcttg cctgcaatcc aaggttccaa tacataaaat gacagactgg
     8941 caaagccata agaacaaatc agagtcagta atacagcaag aagatttgat gtcaactgtg
     9001 agaaaatgat atagaaaggc caatggaaaa gataaaccgc atagctagta tccgctaaaa
     9061 agctgataat cttcggctcc tgtatatgat gtgtcttttc atgtaagaca cgcgccgcca
     9121 gaatcatggc aagagctgca agactggcaa gcaagaagcc gataagatag gcaaaaagat
     9181 aagtgaattt gacaaagaaa gtcaaaagaa ctaggaaacc aaagcctcct ccaaaaacta
     9241 ctaaagtctt tcgtaaatcc cagattttat ccaactgctt gacgagggaa gtcgtctgac
     9301 gaacgcctac aatagttgct aacatacttc ccaaaaagaa tggatagaca tgagttaaac
     9361 tggagaaata aacagaggaa taagaggtca ctagaaaact accaataaac atggagaaga
     9421 aactgatcaa gaaggcagta gcagataaga gaaagatcat ccccttcaac tgaccatttg
     9481 atttagcttg tttggataag aaccaaactg ccaatcccca aagaatatag tagtgaacct
     9541 caactgccaa actccaatta tgaacaaaca aatgaggaat gaactgagat tcataactcc
     9601 cacctgttaa gagttcatag aagttggtca taaagcctaa gacgcccgca atctggccac
     9661 caattccagc aacatagtct tggcgaacca agaaagtaaa aggcatggtc accaagacca
     9721 tcaaaaccac aggtggcaca atccgataaa agcgtcttct aaaaaatcct atcaaatcaa
     9781 tctcattgtt cttagaaaat tcttcaatga gtagagctgt aatcaggaaa cctgaaaatg
     9841 tgaaaaagac atctaccccg aaaaatcctc caggaaagat cgtctgaaag aagtgataca
     9901 agagtaccaa aagtaaacct ataatcctaa tcaaggaaaa ccatttaatg cgcatacgag
     9961 tttattcttc ctttcattgt acactttatt ctatcaaaaa aagaagaaaa atcctaagag
    10021 aaaacttagg atttttagct tatatcaatt ccattttaga aattacgtcc tgacttatta
    10081 tagccatatt tttccacaaa atacttacgg aattccaaga gattgtcatc catgatggct
    10141 tgtcgcactt gcttcatcag gttaagcaag aagtaaagat tgtggtagct agtcaagcgg
    10201 ataccaaagg tttcatcagc cttgagcagg tgacgaaggt aagcgcgtgt atagttatta
    10261 catgtgtagc aatcacactc aggatccagt ggcgtaaagt cctcagcaaa ctgggcattt
    10321 ttcacaacca aacgtccttg actggtcata caagtcccgt tacgagcaat tcgagtcggt
    10381 aagacacagt caaacatatc caccccacga atgaccccat cgatcaagct atctggcgct
    10441 cccacaccca tcagataacg aggtttattt tcaggcagca gttgagttgt aaagtccaag
    10501 accgcattca tctcttcatg ggtttctccc actgccaaac caccgataga gtagcctgag
    10561 aaatccatgc tgacaagatc gtgagctgat tggcggcgaa ggtcttcaaa tcctgcacct
    10621 tgcacaattc caaacaaacc ttggtcatgt ggacgacggt gagccttcaa accacgctca
    10681 gcccaacggc tggtacgctc gatcgatttc ttaacgtagt cataaggttg ataaaactga
    10741 ggacattcat caaaggacat catgatgtct gaacccagat tattctgaat agagatggct
    10801 ttttctgggg ataggagcat cttagaacca tttagatgat ttttaaaggt tactccttct
    10861 tctgtgatat tacggctatc tgctaaagaa taaacctgaa aaccaccact atctgtcaag
    10921 ataggctggt cccaattcat gaacttgtgg agaccaccag cgcgtgcaat gagttcatct
    10981 ccagggcgaa gccagagatg ataggtgttt gataggataa ttcccgaacc catctccttc
    11041 aattcttcag gtgactgagt tttgacagtg gcttgtgtcc caactggcat aaacataggt
    11101 gtcggaaagg taccgtgggg agtgatgatt tctcctagac gagctcctgt gtgtttttct
    11161 ttcttaatca aacgatattt gattggtgaa tctgacattt tttacctccg aagctgggaa
    11221 agacagtccc agttcataca gtgtgcccaa ggacacactg tatgatttta tcaaaaaaag
    11281 taggaactgt cacgaactat gtataagaac tatggtataa taagagaatg aactatccaa
    11341 aaattgattt aaaaacaatt cgtcaggagt caaaacattt tcaggctgac accccccgtc
    11401 tctttctcct ctatatcctt cccagtatgc tggtcatctt atctggtttt cttaatccct
    11461 tatctcggat ccacgggact gttttagaac agccattttt cagcatactt ggccaaatac
    11521 ttcaaaccta cctctttcct ctactagtct cctttatcgg gactattctt ttgaccagtt
    11581 ccgtctatgc aacactgaca ctcatgaaag attctaaaac ggagccatct gtaaaaaaca
    11641 gcctagctct ctttgatgag gagcgctttt ctcaaacctt tttgacccta cttctcaaac
    11701 gtttctatct ctttttatgg agtatcccta atttacttgg gatttacctc ctcttttata
    11761 gtagcttttt agccaaaaaa ttcgtcaccc tacatcctga gtttccaaat ctagatctct
    11821 catcagttga aaccgagcgt ttcctcatgg tctttggact ctactttctt gcaagtctca
    11881 tcttgatcat tgtaggaaat atcctctata ttccacagta ttatgcctat tcacaggtgg
    11941 aatttcttct ctgttatagt ctagacctag gacaggtccc accaagacga atcctaaaaa
    12001 ctagccgttc ctttatgaag ggctacaagt tccagcactt tgtcctagat ttacagcttc
    12061 ttccttggta cttcctcaat tggattacct ttggcattgc tagtttttca ctcctacctt
    12121 atatccaatg cactaaaatt atgttttacc gagcagtatt ggctcgaaaa cgtccaaaag
    12181 cttgaaggtt tttcccctca agcttttttc tatcaatgag tttctccgcc taccaacttg
    12241 agttcctgat ctccatgttc tatcatagcg ccgattgctg cttctatccc tcgccgaata
    12301 tccactaaac tcatagttgg agtagtcggt ctgttcacca cctgttccat catataagga
    12361 atatgcataa aacctgcctt aacatatgga aatttctttt ctaccaaata gagagcctga
    12421 tacatcaaat ggctgcagac aaaagtccct gccgtattgg aaacagaggc cggtaagccc
    12481 tcttttttta tagcttgaac catcgcttta atcggcaaac tactaaagta ggccgaagca
    12541 ccatctgggc gaatgggacg gtcaatcggt tgattatctt cgttatcaga aatgcgtgca
    12601 tcgtcttgat taatggccac tcgttcaagt gtcacactag ttcttccacc agcttgccca
    12661 atacaaagga caaagtcagg ttgataacga ttcatctctt cttccaatac ttgagcagat
    12721 ttgtgaaaaa ctgtcggcac ctctagccaa cggacctcag caccatggat ttcagctggt
    12781 aaacctttaa tggcctccaa agttggattg cccttttccc ctccaaaggg ctcaaaacct
    12841 gtcactaata ctttcatttc taactcctca aaaccaattc aatgagactt ttttcttaaa
    12901 aacaagtata acatatttcc cttattctgg attaaaaagg attagcgaga ttaaaatcga
    12961 ccttcaagtg tatttaaaga gtaaaatact aatcaaggcc aaaatagctg atccaccttg
    13021 cttcaaaata atttttttat ccgctgttaa agagccgtaa gtcgcagcac caatcacaaa
    13081 taagacaaaa atagtcacaa tttctaaatt ctgtgagaaa taaatgccat accagagaaa
    13141 gactcctagc agagccttat aaatcccttg atttttgaac aatgaactta ctgacggatg
    13201 agccaattct tccttttcca tattaaatac acgactagtc gcatctgatc gcgtagcaat
    13261 actttccaaa taaaaaatgt aaaaatgctc caaagcaacg atcgttgtta aaataattgt
    13321 aataattgac atctatttct ccttaaattt ctatattttc aatacccaaa accaatttat
    13381 ttaataaacg gctgagttct gttctctcaa actctgttaa gatactttcc atctcttcct
    13441 taaccttgat atgttgcaga gggggattca ccactaactg ctccttggca tacttcgcag
    13501 cctctaccaa cacttcccgc tgattttcag gattacgatg acgctccacc aaaccttccg
    13561 tttccaaaat cttgaaatgc cgtgtcaaag cagcctgatc aattttcaaa cgctcctgaa
    13621 ccgccatttg gttacaagga gaatgctcca gcaaaaacag taaaatctga taccgtgtca
    13681 aactaatccc aagccttttt tcaaacaatt gcgtactcgc ttgctcagac aagcggagtt
    13741 gatacagtaa atcttgaatc tctatcattt cttcctccta tccattgact tatcaatagt
    13801 tgaccaatca attataatat aaagtatagt gaaatg
//
LOCUS       DAVBCW010000051        12469 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVBCW010000051 DAVBCW010000000
VERSION     DAVBCW010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12469
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.051"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(251..433)
                     /locus_tag="VQ514_001253"
     CDS             complement(251..433)
                     /locus_tag="VQ514_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500009.1"
                     /translation="MAVIFKNVLIEEKTEEEKQHILSILNGEYDLSDDVSCRLRRLNE
                     ESKRLEVFVKETNSLV"
     gene            complement(649..1881)
                     /locus_tag="VQ514_001254"
     CDS             complement(649..1881)
                     /locus_tag="VQ514_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HET2500010.1"
                     /translation="MKIAVAGTGYVGLSIAILLAQHHQVVAVDIIPEKVELINKKKSP
                     IQDDYIEKYLAEKELNLVATLNAEEAYENADFIVIAAPTNYDPKKNFFDTSSVEAVIE
                     AVMKYNPDAIIIIKSTVPVGYTKSVRRKFLTDNIMFSPEFLRESKALYDNLYPSRIII
                     GTDKDDKDLVKSAEIFVKMLQEGAVKENIETLYMDFTEAEAVKLFANTYLALRVSYFN
                     ELDTYSEMKGLDTQAIIDGVCLDPRIGSHYNNPSFGYGGYCLPKDTKQLLANYSDVPQ
                     EMITAIVQSNRTRKDYIADRILDLAGSYVGNDFYKPDKEKEIVVGIYRLTMKSNSDNF
                     RQSSIQGIMKRLKAKGVTIIIYEPTLESGTTFYGSQVVNDFEIFKKSSQVIVANRFDE
                     TLEDVRGKVYTRDIFKRD"
     gene            complement(1910..2980)
                     /gene="gla"
                     /locus_tag="VQ514_001255"
     CDS             complement(1910..2980)
                     /gene="gla"
                     /locus_tag="VQ514_001255"
                     /EC_number="5.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001214397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate 4-epimerase Gla"
                     /protein_id="HET2500011.1"
                     /translation="MRGKTILITGVAGFIGSNLVRKLLELEGTMTIIGLDNLNDYYDI
                     ALKDYRLLQLNKLIQDYPEKNWIFIKGDIADKMLINKIFKSYHPQIVVNLAAQAGVRY
                     SITNPDVYIESNIVGFHNVLEACRHSYEIYDGGVEHLVYASSSSVYGGNKKIPFATED
                     KVDNPISLYAATKKTNELQAYAYSKIYNIPSTGLRFFTVYGPAGRPDMAYFGFTDKLR
                     SGSDIEIYNHGNCKRDFTYIDDIVEGIVRVMQSAPKKLVGSDNLPLAPYKVYNIGNSK
                     PENLLDFVDVLQQELIKAGVLPENYDFDSHKKLVPMQPGDVPVTYADTSDLERDFGFK
                     PKTSLREGLRKFAEWYKDYYNV"
     gene            complement(3048..4217)
                     /locus_tag="VQ514_001256"
     CDS             complement(3048..4217)
                     /locus_tag="VQ514_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HET2500012.1"
                     /translation="MRLSKKYWFALTSKFLLILLTLLSSIIINRGLGVEKKGDYAYVM
                     NLVEIFYILGGVGLGQAYSTFKRNHSNEYQNKFVFLTFIQGLLFGIIGLIIVLADVPI
                     DYGGVIIVLSSLSIMRTNLTMIAVIENSIKRNKIVLGVSVVYSVMLVLLLLFHLVSIE
                     MVFLVYAINEFLRIILFLYYYNMFPVFEHISIKEFFTIYKFGFLSMILTLLITFNYSI
                     DIIMLKQMSSSYNVGIYSVAVTFSNMFLMVPDSFKEILFGDSAKRTFEKNKAINAIKV
                     SFLFMILLIIGFLSFGEFAITLLYGSSYIKAFPITLVIFLGSLSMIFFKILQPIYISQ
                     GKQSVAIKILSVSALINISLNWYLIPNYQITGAAIASAISYTVCGLFFIIDYLRN"
     gene            complement(4403..5707)
                     /locus_tag="VQ514_001257"
     CDS             complement(4403..5707)
                     /locus_tag="VQ514_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HET2500013.1"
                     /translation="MKFSLQSNDIKNKIILLVGVVFSGTLILCSENNDVTIPLFSMIF
                     LSFMAMIIVKFNIFHPYVWYSSFFTIYSISYPLLYRMEYINYGYTKDLIFMQWLALAT
                     LLLTLPSKAIVLSERKINLNNNNYLLFLNNMNTFITTVAIIYLLGSGFRNKGEIYSGA
                     NIVIMLVFSLVYFMILIHTYQLFIQLSTKKWRLLNCNTNTAIVICLFSIVTGERDYMF
                     TLFILDVIILFYFHKIKRFYLVMLVPLAALLIPLSAVFKYTLLSGQVSSINTNNIWFD
                     LLDGEFVSASRNLQILILHNMGNYFEGRSFFNDIVRIFYNTGYSNQTWFMDTFFPNVH
                     STKYGFTLVGEGYVNGGYFGIVMIFMLTGFLMRFLYINAQRNIYGMLIYLYMIPIFIY
                     STRADFANILSPLLKYAILGTLVIVFINRMVLISPLDEMGGE"
     gene            complement(5824..6978)
                     /locus_tag="VQ514_001258"
     CDS             complement(5824..6978)
                     /locus_tag="VQ514_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET2500014.1"
                     /translation="MKVFIATEFRCTIYKNEIYLKPKAYFIYERYAKAFGEIVLCSRY
                     EKVESYPEGYHKANFINKFIPIEGLHQVLFGQNKNKIIEGMIDSDLIVVRIPSIIGSK
                     TADYALKIGKPYLTEIMGDAWDSYWYHSLKGKLLAPYIYAKTKSIVKNANYCIYVTEK
                     YLQDRYPNIKSNIVASNVNITSVENRSLKSRLYKLKKFNPQKISIMTTASVNVRAKGH
                     RFVLEAMKRLEIQGILLDYYLAGDGDQSFLKKKAEELGVANRIHFLGELTTSQIYEYL
                     DKVDLYIQPSLQEGLPRAVIEAMSRACPCIGSNKAGIPELLGPHCLFTPSSSQAIAGS
                     IIDFISMDKEKMVIKQFEFSKKFLSSVLNKRRSDYFAQIRRELNNSDGLD"
     gene            complement(6975..7526)
                     /locus_tag="VQ514_001259"
     CDS             complement(6975..7526)
                     /locus_tag="VQ514_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET2500015.1"
                     /translation="MNRIIKNIVGLLRANICLILLKVCRNKDLTFHYFLKFGKNVTVE
                     QESNAKLILGKKIRVNAGGVLKVRKGAKLKISDDVFLSNNCMIACRKYIDIKSGVKCG
                     PGVLIYDHDYDVSVPGGLKAKKFKTAPVMIGENVWIGANSIVLKGVSIGENSVVAAGS
                     VVTKDIPADTIFIQKRLSREMKL"
     gene            complement(7519..8655)
                     /locus_tag="VQ514_001260"
     CDS             complement(7519..8655)
                     /locus_tag="VQ514_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET2500016.1"
                     /translation="MNLQKKKIMLITNHDDDIYCFRKEVIESLVEYGYEVLVSCPYGD
                     KIELMKSIQFEYEDITIDRRGINPLKDIRLFLSYIRLLRKYRPDVVLTYTVKPNVYAS
                     LAATILGIKYINNVTGLGSVLTMGSCVRSLVLLLFRISFRKSNCVFFQNEYNMKMLKT
                     LGLILGDSKLIPGSGVNIEKYPVQQYPNGGNGIQGETIVFNFIGRILKEKGIDTYLAA
                     AQIIKSRYPKTEFNIIGFIEPTESNYELKICDLEKKGIVYYLGQQKDVIPHITRSHAI
                     IHPSVYGEGMSNVLLENASSGRVLITTDNPGCKEIVKDRETGYIFQGGNVEELVSILE
                     VFLGLENEKRKEMGLQGRMYIEQYFSRQIIIDAYLDKIKGALDE"
     gene            complement(8796..9461)
                     /gene="cpsD"
                     /locus_tag="VQ514_001261"
     CDS             complement(8796..9461)
                     /gene="cpsD"
                     /locus_tag="VQ514_001261"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase
                     CpsD"
                     /protein_id="HET2500017.1"
                     /translation="MPTLEIAQKKLEFIKKAEEYYNTLCTNIQLSGDKLKVISVTSVN
                     PGEGKTTTSVNIAMSFARAGYKTLLFDGDIRNSVMSGFFKSREKITGLTEFLSGTADL
                     SHGLCDTNIENLFVVQSGSVSPNPTALLQSKNFNDMIETLRKYFDYIIVDTPPIGIVI
                     DAAIITQKCDASILITATGEANKRDVQKAKQQLEQTGELFLGVVLNKLDISVDKYGVY
                     GRK"
     gene            complement(9471..10163)
                     /gene="cpsC"
                     /locus_tag="VQ514_001262"
     CDS             complement(9471..10163)
                     /gene="cpsC"
                     /locus_tag="VQ514_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HET2500018.1"
                     /translation="MKEQNTLEIDVLQLFRALWKRKLVILLVAIITSSVAFAYSTFVI
                     KPEFTSTTRIYVVNRNQGEKPGLTNQDLQAGAYLVKDYREIVLSQDVLEEVISDLKLD
                     LMPKGLANKIKVTVPVDTRIVSVSVSDRVPEEASRIANSLREVAAQKIISITRVSDVT
                     TLEEARPAISPSSPNIKRNTLIGFLAGVIGTSVIVLLLELLDTRVKRPEDIENALQMT
                     LLGVVPNLGKLK"
     gene            complement(10172..10903)
                     /locus_tag="VQ514_001263"
     CDS             complement(10172..10903)
                     /locus_tag="VQ514_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase CpsB"
                     /protein_id="HET2500019.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAESYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVADDLVIAYGAEIYYTLDALEKLEKKEIPTLNDS
                     RYALIEFSMHTSYRQIHTGLSNILMLGITPVIAHIERYDALENNEKRVRELIDMGCYT
                     QINSYHVSKPKFFGEKYKFMKKRARYFLERDLVHVVASDMHNLDSRPPYMQQAYDIIA
                     KKYGAKKAKELFVDNPRKIIMDQLI"
     gene            complement(10905..12350)
                     /locus_tag="VQ514_001264"
     CDS             complement(10905..12350)
                     /locus_tag="VQ514_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET2500020.1"
                     /translation="MSRRFKKSRSQKVKRSVNIVLLTIYLLLICFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLLFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSKIENVTQLTSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     VNQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADS
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKMIDLLGGVDVHNDQ
                     EFSALHGKFHFPVGNVHLDSEQALGFVRERYSLTDGDRDRGRNQQKVIVAIIQKLTST
                     EVLKNYSSILQGLQDSLQTNIPIETMIDLVNTQLESGGNYKVNSQDLKGTGRMDLPSY
                     AMPDSNLYVMEIDDSSLTVVKAAIQDVMEGR"
BASE COUNT         4192 a         2422 c         1640 g         4215 t
ORIGIN      
        1 aattcactta cttcatatta catttagcat gaaatgaaat aagcgaattc tactttgttt
       61 gcatacaatt aaaaatcaat tcctaaggat gttttaaata actgtgtaaa aggcataatt
      121 tcaccatttc ttagtttttc aatgctaaat tacatagtaa ctggagagct attagaaatc
      181 tctaacttcc tcatagttaa aaaatgacaa tgagatactt attgatttca attcattaac
      241 agaatacgaa ctagacaagt gagttcgtct ccttaacaaa cacttccaag cgtttacttt
      301 cctcattcag tcgtcgtaat ctacaactta catcatcaga taagtcatac tcaccattta
      361 gaatagatag aatatgttgt ttttcttctt cagtcttctc ttcaataagc acatttttaa
      421 aaattactgc cattatcagc acctcacttc gaaggaatat atgcaactat atcctattaa
      481 gtcaatctct actgacaaaa gaagtatatt tacttcacta agtgcgagcg agaatataga
      541 aatatcttta ttgttttaga tgagttgtcg ctatcctcca gctagttttt aactattcta
      601 tcattcaaat gatatagatt attagggact agaaatatac aaattgattt aatctctctt
      661 aaaaatatcc ctagtataaa ctttacccct cacatcttct aaagtctcat caaaacgatt
      721 tgcaacaatc acttgactag atttcttaaa tatttcaaaa tcattaacta cttgactacc
      781 atagaaagta gtaccacttt caagagttgg ttcgtaaata ataatagtaa caccttttgc
      841 tttcaaacgc ttcataatac cttgaataga agattgtcta aaattatctg aattactttt
      901 cattgttaaa cgataaattc ctacaacaat ctctttttct ttatctggtt tatagaaatc
      961 attgcctaca taagatccag caagatctaa aatacgatct gcaatataat cttttcttgt
     1021 ccgattactt tgtactatag ctgttatcat ttcttgtgga acgtcagaat aatttgcaag
     1081 aagttgcttg gtatctttag gaagacagta acccccataa ccaaaagaag gattattgta
     1141 gtgagaacct attctcggat ctaaacaaac tccatcaata atcgcttgag tatctagtcc
     1201 tttcatctca gaataagtat ctaactcgtt aaaataggaa acccggaggg caagataggt
     1261 attagcaaaa agttttactg cttcagcttc tgtaaaatcc atatacaatg tttcaatatt
     1321 ttctttcact gcaccttcct gaagcatttt tacaaaaatt tcagcagact ttaccaaatc
     1381 tttatcatct ttatctgtac ctataataat tcgactcgga tataggttat catataacgc
     1441 ttttgactcc cgtaaaaact caggactaaa cataatattg tctgtaagaa atttcctccg
     1501 tactgacttt gtatatccaa caggaacagt acttttaatt attataattg catcaggatt
     1561 atatttcata actgcttcaa tgacagcctc gactgaagat gtatcgaaaa aatttttttt
     1621 cggatcataa ttagttggag ccgcaataac tataaaatcc gcattctcat atgcttcctc
     1681 tgcattcaat gtagctacga gattcaattc tttttccgcg agatattttt ctatataatc
     1741 gtcttggata ggggacttct ttttattgat taactcaact ttctcaggaa tgatatctac
     1801 ggcaacaact tgatgatgtt gagccagtaa gattgcaata gataatccaa catatcctgt
     1861 acctgctact gctattttca taattttttc ctttctcaaa acactctcgt tatacattat
     1921 agtagtcttt ataccactct gcaaactttc tcaaaccttc tcgtaaagaa gtttttggtt
     1981 taaaaccaaa atctctctct aaatcacttg tatctgcata agtaacagga acatcgcctg
     2041 gttgcatagg aactaatttt ttatgactat caaaatcata attttccggc aaaactcctg
     2101 ccttgattag ctcctgttga agaacatcca caaaatctaa caaattttcg ggtttactat
     2161 tcccaatatt gtacacttta tatggggcta acggtaaatt atcagatccc actagtttct
     2221 tcggagctga ttgcatgacc cttactatcc cctcaacgat atcatctata taagtaaaat
     2281 ctcgtttaca attaccatga ttataaatct caatatcgga accacttcgc aatttatctg
     2341 taaatccaaa ataggccata tctgggcgcc cagcaggacc ataaactgta aaaaagcgta
     2401 agcctgtcga aggaatattg taaatctttg aataagcata cgcttgaagt tcatttgttt
     2461 ttttagtcgc agcatatagt gaaataggat tgtctacctt atcttctgtt gcaaacggga
     2521 tttttttatt tccaccatat accgatgagg acgatgcata aactaagtgt tccacaccac
     2581 catcatatat ctcatacgaa tgacgacaag cttccaacac attatgaaag ccaacgatat
     2641 tactttctat atatacatct ggatttgtaa tggagtagcg tactcctgcc tgggcagcta
     2701 gattaacaac aatctgagga tgataactct taaaaatctt attaataagc attttatctg
     2761 caatatcacc cttgataaat atccagtttt tttctggata atcctgaatt agcttattaa
     2821 gttgaagcaa tcgataatct ttaagtgcaa tatcatagta atcattcaaa ttgtccaaac
     2881 caatgattgt catcgttcct tctaattcca ataatttcct aaccagattt gatccaataa
     2941 aaccagctac tcctgtaatt agaattgttt ttcctctcat atcataatac ctcttgttat
     3001 ttttatattt tcgtccacta atacacattc tttaacaact attataatca atttctaaga
     3061 taatctataa taaaaaacaa accacataca gtataagaga ttgctgatgc tatcgcagct
     3121 cctgtaattt ggtagttagg aattaagtac cagtttaaac ttatatttat caaagccgac
     3181 acagataaaa tttttatagc tacactctgt ttaccctgag atatataaat tggttgtaaa
     3241 attttaaaga aaatcatcga taagcttcct aaaaaaatga ctaatgttat aggaaatgcc
     3301 tttatataac tggaaccata aaggagcgta atcgcaaatt ctccaaaaga aagaaatcca
     3361 ataattaata aaatcataaa tagaaatgat acttttattg catttattgc tttatttttc
     3421 tcaaaagttc ttttagccga atcaccaaat aaaatctctt taaatgagtc tggaaccatc
     3481 aaaaacatat tagaaaacgt aactgcgaca ctgtatatac ctacattata actactagac
     3541 atttgcttca acattatgat atcaatacta taattaaatg taattaatag agtaagaatc
     3601 atagaaagaa atccaaactt atatatagtg aaaaattctt taatactaat atgttcaaaa
     3661 actgggaaca tattgtaata gtataaaaat aaaatgatac gtaaaaattc attaatagca
     3721 tataccaaaa ataccatctc tatagaaact aaatgaaata gtaacaacaa tactagcata
     3781 acggaatata ctacgcttac tcctaataca atcttattcc tctttatact attttctatt
     3841 actgcaatca tagtaagatt tgttctcata atagatagac tggacaaaac tattattacc
     3901 ccaccataat ctatcggaac atcagctaaa actattatta aacctattat tccaaacaat
     3961 aatccttgaa taaaagttaa aaaaacaaat ttattctgat actcattaga atgattcctt
     4021 ttaaaggtcg agtaagcctg acctaaacca actccaccta aaatataaaa aatttctact
     4081 aagttcatta cataggcata atctcctttt ttttcaacac ctaatcccct attaattata
     4141 attgaggata aaagtgttaa taagattaac aaaaatttac tcgttaaagc aaaccaatat
     4201 ttcttagata gtctcactta ttttctccgt atgttaaatt ttataacagt aaatttaaaa
     4261 ttatagtata ttgaaataga atatgaacaa aacgattagg gaatttaaag taatttataa
     4321 aaattcttta gaagctgcat tgtactactc caacttcaaa ccactatagt atcaaaaagt
     4381 ataccccacc tttttaaata aactactcac cacccatttc atccaatggt gatatcaaca
     4441 ccattctatt gataaaaaca ataaccaatg ttcccaatat agcatattta agtaatggag
     4501 ataatatgtt agcaaaatca gcacgagtag aataaataaa aataggtatc atatacagat
     4561 atatcagcat cccgtatata tttctttgag cgtttatata aagaaatctc atcaaaaagc
     4621 ctgttagcat aaaaatcatt acaattccaa agtatcctcc attcacatat ccctctccca
     4681 caagagtaaa accatatttg gttgaatgta cgtttggaaa aaaggtgtcc ataaaccaag
     4741 tttgattaga ataccccgta ttataaaaaa ttcttactat gtcattaaaa aaagaacgac
     4801 cttcgaaata attgcccata ttatgtaata tcagaatctg taaattacga ctagctgata
     4861 caaactcacc atctagcaaa tcaaaccaaa tattatttgt atttatagat gaaacttgcc
     4921 cacttaataa agtatattta aaaactgcag ataacggaat cagtagtgcc gcaagcggaa
     4981 ctaacattac aagataaaaa cgttttattt tgtgaaagta aaataatata atcacatcaa
     5041 ggatgaataa agtaaacata taatctcttt cccctgtaac gatagagaag agacagataa
     5101 ctatcgctgt gttagtatta caatttagta gtctccattt tttagtactc aactgtataa
     5161 atagctgata tgtatgaatg agaatcataa aataaaccaa agaaaatact aacattatca
     5221 caatattggc acctgaatat atttcccctt tatttctaaa accacttcct aatagatata
     5281 taattgctac tgttgtgata aacgtattca tattattgag aaataataaa tagttattgt
     5341 tatttaggtt tattttcctt tcgcttaaca caatggcttt agaaggtaga gttaacaaca
     5401 aagttgccaa agccagccat tgcatgaaaa tcaaatcttt tgtataacca tagtttatat
     5461 actccattct atagagtagc gggtaagaaa tagaataaat agtaaagaag ctactatacc
     5521 acacataggg atgaaaaata ttaaatttca caattatcat tgccataaaa cttaaaaaaa
     5581 tcattgaaaa taatggaatt gtaacatcat tattttcact gcataaaatt aaagtccctg
     5641 aaaaaactac acctactaga agtattattt tattttttat atcatttgat tgtagactaa
     5701 acttcattta cttttttcct ttaactaata atttaaacgt aatgcgtatt caaaaatttg
     5761 attggctatg tagtcagttt ttatagcata tttaatatat cgaaacggat tttccatcgc
     5821 attttaatct aaaccatcag aattatttaa ttctcttcta atttgagcaa aatagtcact
     5881 tctgcgttta tttaatacac tcgaaagaaa tttttttgag aattcaaact gttttatcac
     5941 catcttttcc ttatccatac taataaaatc tataatagat cccgcaatcg cttgtgaaga
     6001 ggatggagta aatagacaat gtggccccag caactctgga attcctgctt tattagaccc
     6061 tatacaaggg caagcacggc tcatagcttc aattaccgca cgaggtaacc cttcttgtaa
     6121 acttggttgt atatataaat caactttatc caaatactca taaatttgtg atgtagtcaa
     6181 ttcccctaaa aaatggattc tattcgctac tcccaattcc tctgctttct tttttaagaa
     6241 actttgatca ccatcacctg ctaaataata atccaacaaa ataccttgta tttctaatct
     6301 cttcattgct tccaatacaa atctatggcc cttggctcgt acattcacag atgctgttgt
     6361 cattattgaa attttttgag gattaaattt tttcaactta taaagacggc tcttcaaaga
     6421 tctattctct acagaggtaa tattaacatt tgaagcaacg atattagatt taatattagg
     6481 atatctatct tgtaaatatt tttctgtcac gtatatgcaa taattagcgt tttttacaat
     6541 tgatttagtt ttggcgtata tatatggagc taataatttt ccctttaaac tatgatacca
     6601 gtaagaatcc caagcatccc ccattatttc tgtcagatac ggcttaccta tcttcaatgc
     6661 gtagtctgca gtttttgatc caattataga cggaatacga acaactatta agtcgctatc
     6721 aatcattcct tcaataatct tatttttatt ttgaccaaat agaacctgat gtagaccttc
     6781 tatagggata aatttattaa taaagttcgc tttatgatat ccttcagggt atgattccac
     6841 cttctcataa cgactacata gtacaatctc tccaaatgct ttagcatagc gctcataaat
     6901 aaaataagct ttcggtttta aatatatttc atttttatag attgtacatc taaattcagt
     6961 cgctataaat actttcataa tttcatctcc cttgataaac gtttctgaat aaatatagta
     7021 tcagctggaa tatcctttgt tacaacactt cctgctgcaa ccacactatt ctcaccaata
     7081 ctcactccct tcaagacaat gctgttagct ccaatccaaa cattttctcc aatcataact
     7141 ggggccgtct taaatttttt tgctttcaat ccacctggaa cactaacatc atagtcatga
     7201 tcatatataa gtactccagg accacatttt actccagatt taatatctat gtatttacga
     7261 caagctatca tacaattatt actcaaaaat acatcatcag aaatcttgag ttttgctcct
     7321 tttctaactt tcaatactcc cccggcgttt actctaatct tttttcctaa gattaattta
     7381 gcattagatt cctgttcgac agttacattc tttccaaatt ttaaaaaata atgaaaagta
     7441 agatccttgt ttcgacacac ttttaataat atcaagcaaa tatttgcacg aagtaaacca
     7501 acaatatttt ttataatcct attcatctaa agctcctttt attttatcta aataagcatc
     7561 tataataatt tgacgagaaa aatattgctc tatatacatt ctcccttgaa gtcccatctc
     7621 ttttcgtttt tcattttcta gacctaaaaa aacttccaat atagagacta gttcctcaac
     7681 atttccccct tgaaatatat agcctgtctc tctatcttta acaatttctt tgcaacctgg
     7741 attatctgtc gtaattaaaa cacgtcctga actagcgttt tctagtaata cattgctcat
     7801 tccttcacca tacacactgg gatggataat tgcatgggaa cgggtaatat gaggtatcac
     7861 atctttttgt tgtcccaaat aataaacgat tccttttttt tctaagtcac aaattttaag
     7921 ttcataatta ctctctgtcg gttctataaa gccaataata ttaaactctg ttttgggata
     7981 tcgactctta ataatttggg cagcagccag ataagtatct ataccttttt cttttaatat
     8041 cctaccaata aaattaaaaa cgattgtttc tccttgaatt ccatttccac catttggata
     8101 ctgttgcaca ggatactttt caatgttcac tcctgaccca ggtatcagct tagagtcacc
     8161 caaaatcaag ccaagagttt ttaacatttt catattatat tcattttgaa aaaaaacaca
     8221 atttgatttc ctaaaagaaa ttctaaacag taataacacc aggcttctta cacaagaacc
     8281 cattgttaag acactaccta aacctgttac gttatttata tattttattc ctaaaattgt
     8341 tgcagctagg ctagcatata catttggttt cacggtatat gtcaagacta catccggtct
     8401 atattttctc aataatctta tataactaag aaatagacga atatctttta aagggtttat
     8461 tcctcttcta tcaatagtaa tatcttcata ctcaaattga atagacttca tcaactctat
     8521 cttatctcca taaggacatg acactagcac ttcataccca tactcaacta gtgactctat
     8581 aacttctttt cgaaaacaat agatatcatc gtcatgatta gttattaaca taattttttt
     8641 cttttgtaaa ttcatcaaaa taatcttcct aaaatataat taaccgctac tccattatcc
     8701 tttacaacta tcattttaaa aatagtaatt ttctataagt aattgtttcc aaattgtgac
     8761 actttaaaat tctatacaca tatagaactt acaaactatt tacgaccgta aactccatac
     8821 ttatcaaccg agatatccaa tttatttaaa acaactccca ggaacagttc ccctgtttgt
     8881 tctaattgtt gtttcgcttt ttggacatca cgtttattcg cctcacctgt tgctgttatc
     8941 aagatggacg catcacactt ttgagtgata attgccgcat caataacaat tccaataggt
     9001 ggtgtatcaa caatgatata atcaaaatat ttacgcaatg tttcaatcat atcattaaaa
     9061 tttttacttt gtaacaaggc tgtagggttt ggtgatacag atcccgattg aactacaaat
     9121 aaattttcaa tatttgtatc acataaaccg tgagataaat cagctgtccc agataaaaat
     9181 tctgttagcc ctgtaatttt ttcacgagat ttaaaaaatc ctgacataac tgaatttcga
     9241 atatcgccat cgaacaaaag agttttatag cctgcacgcg caaatgacat tgctatattt
     9301 acggaagtag ttgtttttcc ttcccccgga ttaacagaag taacggaaat tacttttagt
     9361 ttatctccgc tcaactgtat atttgtacac aaggtattgt aatattcttc tgccttctta
     9421 atgaactcca gttttttttg tgctatttct aatgtcggca tctctctctc ctatttcaac
     9481 ttacccaagt ttggtacaac tcccaaaagt gtcatctgca gtgcattttc gatatcttcc
     9541 ggacgtttca cacgagtgtc caaaagttca agaagaagaa ctataacact agttccaatc
     9601 acccctgcca aaaaaccaat tagtgtattg cgtttaatat ttggcgaaga cggtgatatc
     9661 gccggtcttg cctcctccag tgttgtcaca tcagaaacac gagtaatact gataattttt
     9721 tgagcagcta cttctcttaa agagttagcg atacggcttg cctcttcagg aactcgatca
     9781 ctaactgaaa cagagacaat acgggtatca actggtactg ttactttaat tttattagcc
     9841 aaaccttttg gcatcaaatc tagtttcaaa tcagaaataa cttcctccaa aacatcctgc
     9901 gataggacaa tttcacgata gtctttaacc aagtatgctc ctgcctgcaa gtcttgattg
     9961 gttaaaccag gcttctctcc ctgattacgg ttaactacat aaatccgagt cgtactagta
    10021 aactcaggtt tgataacaaa agtactgtag gcaaaagcaa ctgaagaagt tataattgcc
    10081 actaataaaa tgaccaactt tcttttccat aaagctctga atagttgcaa tacatcgatt
    10141 tccaaagtgt tttgttcctt catattttct cctaaattaa ttgatccatt ataatttttc
    10201 tgggattatc tacaaaaagt tctttcgctt ttttcgctcc atatttctta gcaatgatat
    10261 catatgcctg ttgcatatat ggaggtctac tgtctaaatt gtgcatgtca cttgcaacta
    10321 catgaactaa atcacgttcc aaaaaatacc gagctctctt tttcatgaat ttatattttt
    10381 caccaaagaa cttaggtttt gaaacatgat aactatttat ctgagtatag caccccatat
    10441 caatcagttc acgaacacgt ttttcgttat tctctaaagc atcataacgt tcaatatgag
    10501 caattactgg cgtgattccc aacatcaaaa tattgctcaa tcccgtatga atctgacgat
    10561 aggaagtatg catgctaaac tcaatcaagg cataacgact atcattaagg gtaggaattt
    10621 cttttttttc tagcttttct agagcatcca gagtatagta tatctctgcg ccataagcaa
    10681 tgactaaatc atctgctact tcttttgcaa tttcccgaac ctgaagaaag ttttctgcta
    10741 tcttctcttc cggagtttca aacatgccct tgcgacggtg agaggtagag acaatggttc
    10801 tcaccccctg cctgtaggat tctgccaaga gagccttgct ttcctctctt gacttgggac
    10861 catcatctac atcaaagacg atgtgcgaat ggatgtctat cattttatct accctccatc
    10921 acatcctgta tagctgcttt aactacagtt aaactactat catctatttc catcacatag
    10981 aggttactgt ctggcattgc ataagaagga agatccatcc gacctgtccc ttttaaatct
    11041 tgagaattta ctttataatt ccctccactt tccaactgag tattcactaa atctatcata
    11101 gtctcaatcg gtatatttgt ttgaagagaa tcctgcaatc cttgaagaat actactatag
    11161 tttttcaaaa cctctgtaga agttaacttc tgaataattg ctacaatgac cttttgttgg
    11221 ttgcgaccac ggtcacggtc tccatcggtt agtgagtagc gttcacgtac aaaacctaaa
    11281 gcttgctcag agtctagatg gacattccct actgggaaat ggaacttccc atgtagagct
    11341 gaaaactctt gatcattatg aacatctacc cctcccaata agtcaatcat tttcaagaaa
    11401 gaagtgaagt tcaatcgcac atagtaattg atatccactc catagagatt ttctaaggtg
    11461 tgaatggacg aatcaactcc ataaatgccc gcatgggtta atttatcttt ttgattattt
    11521 ccactatctg cgattggtac ataggcatca cgtggcgttg tggtcaagag gattttcttg
    11581 gtatctcgat tgacagtcat taggatattg acatctgatc gcgacaccga actaatagga
    11641 ccataggtgt caattccact aacatagata ttgaaagact gattcttaga cgtcttagga
    11701 gcctctactt ttttagtgaa tcccttggta taaatctttt ttatcttcga tgcgtagtct
    11761 ggatactctg actcgatgat attttcaaag acactattta ggacaatggc cttagtctcc
    11821 cctgcaatca aactcttgta agctgccaag taagacgaac tctggttgac cgtcaaatcg
    11881 gtattctgac ttgacttgat atcagctagt agtttttgaa tattttcatt atcagtccca
    11941 gtcggtgctg tcacactcgt cagttgcgta acattttcga tcttactatc tgctaaaaca
    12001 gcgacactga ttgaatattc tgagtaatta gaagtcgcat ttaaacgatt ggtcagtcca
    12061 acaaactgct gtactgcaaa gagcgacact gagctgacaa ggatagagaa cagcaacaga
    12121 aaaatagtaa acttttcagc ttttttatag ataatcaaga gtagccctac caaggcaact
    12181 agtaggacta acgcagttac cactaggtta agatatctaa aagcaaggat attgtactta
    12241 aagattaaga acaataaaaa acaaattaac aataaataaa tagtcagcaa aacgatatta
    12301 acacttcgct tcactttctg tgaacgtgat tttttaaaac gtctactcat gattaacacc
    12361 tataccttga acatcataca attatatcac ttttttacgg taatgtctac acctttattt
    12421 tcactatctg catctttaag tatcttagta gacttcccgc gaaccaaaa
//
LOCUS       DAVBCW010000052        12469 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVBCW010000052 DAVBCW010000000
VERSION     DAVBCW010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12469
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.052"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(1..134)
                     /locus_tag="VQ514_001265"
                     /pseudo
     CDS             complement(1..134)
                     /locus_tag="VQ514_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(196..645)
                     /locus_tag="VQ514_001266"
     CDS             complement(196..645)
                     /locus_tag="VQ514_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HET2500021.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(647..1405)
                     /locus_tag="VQ514_001267"
     CDS             complement(647..1405)
                     /locus_tag="VQ514_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HET2500022.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(1520..3499)
                     /gene="pknB"
                     /locus_tag="VQ514_001268"
     CDS             complement(1520..3499)
                     /gene="pknB"
                     /locus_tag="VQ514_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HET2500023.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSVE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(3496..4236)
                     /locus_tag="VQ514_001269"
     CDS             complement(3496..4236)
                     /locus_tag="VQ514_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HET2500024.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVFMNEEDAE"
     gene            complement(4251..5564)
                     /gene="rsmB"
                     /locus_tag="VQ514_001270"
     CDS             complement(4251..5564)
                     /gene="rsmB"
                     /locus_tag="VQ514_001270"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HET2500025.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLSAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANNSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVEDRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(5557..6492)
                     /locus_tag="VQ514_001271"
     CDS             complement(5557..6492)
                     /locus_tag="VQ514_001271"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HET2500026.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEELMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(6505..8901)
                     /locus_tag="VQ514_001272"
     CDS             complement(6505..8901)
                     /locus_tag="VQ514_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HET2500027.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLKSQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKSMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGTVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNSQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(8967..9281)
                     /locus_tag="VQ514_001273"
     CDS             complement(8967..9281)
                     /locus_tag="VQ514_001273"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HET2500028.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(9306..9932)
                     /gene="gmk"
                     /locus_tag="VQ514_001274"
     CDS             complement(9306..9932)
                     /gene="gmk"
                     /locus_tag="VQ514_001274"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HET2500029.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(10062..11675)
                     /locus_tag="VQ514_001275"
     CDS             complement(10062..11675)
                     /locus_tag="VQ514_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HET2500030.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            complement(11866..12120)
                     /locus_tag="VQ514_001276"
                     /pseudo
     CDS             complement(11866..12120)
                     /locus_tag="VQ514_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152757.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
     gene            complement(12124..12378)
                     /locus_tag="VQ514_001277"
     CDS             complement(12124..12378)
                     /locus_tag="VQ514_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HET2500031.1"
                     /translation="MEAVLYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVLSKS
                     EWDSIQETLRIAQNKELSDKVLRGMAQVRAGSTQVHVIEE"
BASE COUNT         3317 a         2974 c         2473 g         3705 t
ORIGIN      
        1 gaggagttct tgaaatcgta acaccacctt tgaacaagag ttacttcaac ccattggctc
       61 cgacggagta agttgctttc gtggatacca aaatcagccg caatttcttc ataagtgcgg
      121 tattctcgca catatagaaa gcgttgttaa tttatttatc tcatttttca gaaaattctt
      181 ttatttctgt aaagtctacg atactcgatg tgtttttata taatgataca gtctgagaat
      241 cactgttccg ctagccattc caatagagat taccaaagcc aacatgacaa ccaaggtcgc
      301 acttgccagt gctttattat agtcccctgt cacaaaaaag gcagttgttc ggtaggagag
      361 ataacctgga accagcggtg ccaaaatggc caagataaag accacagcag gtgtcttata
      421 aagaatactt aaaatctggc tgacacaaga accaataatg gctgcaatga aggtagctac
      481 aatgacattg gtcggttcct tgagcaagag atagattagc cagacagtca tgcccaaaat
      541 ccctccaggt aagagcatag accgttgcac attgagtacg attaaaaaag tgataatggc
      601 aagaaaactt gctactgctt gtaataaaaa ggttgttagt gtcatattag ttcatcaata
      661 ccaaggcgac agaagttcct gcccctaaag cgagggtaat gagcagggat tcaaacatct
      721 tactcatacc agagtttatg tggttggtca taatatcacg gaccgcattg gtcaaggcaa
      781 tacctggtac aaacggcatg accgcaccag ctataatcaa atctgccgtt gaaggaaaac
      841 ctgtgtagcg agcccaaaac tgggcaatta tcccaaagac aaaggctcca gcaaaggctg
      901 tcacaaaggg aattcggata aatttttcca catagaggga aaaggcaaaa ccaaataagg
      961 tcgccactcc tgccccaagt gcgtcgtaga tatttccgct aaacataact gaaaagaaag
     1021 gagcactaaa ggtcgcagcc agagttacct gcaacttagt atagggaagg ggttgagctt
     1081 gcaaggccgt caattgctta aaggctgttt ctaagtcaat ctgcccccca actagctgac
     1141 gagaaatctg gttcacatcg cagacttttt cgatgttata agaagaggag gtcacgcgct
     1201 tcatgcgcga aatattggta ttttcaatag agaaaaagat agcggcaggc atggcaagga
     1261 cattgcaatc cacaatcccc tgcgaatgcg cgattcgaat catggtatct tctacacgat
     1321 ggatttctga gccactttta aggagaatag tccccgctag cataatcaca tcaatgacgg
     1381 catttaattc ttttgattct tccatgcttt cctcctttta tcaactccct ctattctatc
     1441 acaaatccgg actcaaaaaa aatctttgcc atgaaatcat gacaaagatt gattactcat
     1501 tttgattatc catctgcttt taaggagtag ctgaagttgt tttaggtttg tagattgaaa
     1561 tcttgactct agtcttattg aggtctacct tttcacctgc tctaggactt tgttcaacaa
     1621 ccatgccttc tacactacct gcaggcgctg tcgtcacttc tacaacttct atattagctt
     1681 ccttaatccc aacaatttga atcaaattgt tcttagtaaa ctccaagctg gaaccaatgt
     1741 aactcggcat ggcaacactt gtaacttttt tagctactgt caagacaatt tgagtaggtt
     1801 tactcacatc ataagtcgtt ccggcacctg gactttgttt cataatcgtt cctggttcgc
     1861 tttcgctgga ctcttcttcc tctatcttaa tcaaattctc aggaaccttc ttctgcttga
     1921 gttctgagat tacttctgta gagttccgtc caatatagtt ccctaattga atcgtcgtag
     1981 cttttttagc tactgtcaaa acaatttgag ttgccttgct caagtcatag gtcgtacctt
     2041 ctggtagact ttgcttcagg accgttccag cctcactctc attcgactct tcttcctcaa
     2101 ttttaatcaa attatctgga acttttttct cttttaattc cgcgataaca tcagaagatt
     2161 tccggccgac ataattacta atttggaagg attgtttgcc tgatgagaca accagattga
     2221 ttttcgttcc ttcttttcga ccagttccag cgccaggatc tgtacggata atccgccctt
     2281 cttccacctt ttcactagcc tctgtcttct cctcaccaat ctcaaaattg gcttttttga
     2341 gcgttgcctt ggcctctgca actgtctgac ctgccacatc tggaatggca atggttgcag
     2401 gagttctgga tagtatccaa ataagagaag ctgccaccaa tacaaggctg gccaacaaaa
     2461 tcaggtaacg catcttaaat ctatgttttt tcggtgcttg tggttggtaa gtttcctctg
     2521 tcacagcctg gcttgggttt ttgattgatt tgtgttctgt ctgcgcttga accttaggaa
     2581 tagatgtcaa ggtactctga gaaaccttcg gcaaggtctt ggtatctgcc ttgctcgttt
     2641 catcaaagat taacttactt tcatttctac gattgtagga caagctacta gacaagtcca
     2701 catacatctc tgaaaccgag cggtagcgat tggtcaactt tttagcagtt gccttgataa
     2761 taacattttc taaagcctga ggtacagatg gattttctgc aataacggac ggcaggggtt
     2821 tctggaaatg ctggagggca atggtcaccg cgctatcccc gtcataaggg atatggcctg
     2881 tcaacatctc atagaaaata atccccatgg catagatatc actctgcaca gtcgccttcg
     2941 aaccacgcgc ctgctctggt gacaagtaat gaactgagcc caacatcgag ttagtctggg
     3001 tcagacttgt ctctgcaaag gctacagcaa tcccaaagtc tgtgaccttg gcagtcccat
     3061 ctggtgtcaa aaggatattt tgaggtttca agtccctgtg aacaattcct cgagtatggg
     3121 ccaagcgcat agccaagaga atttgtccca tgatacggac tgcttcttca ttagaaagag
     3181 gataatgttc cttgatatag cgtttgaggt ccagtccagc cacatactcc atagctaggt
     3241 actgttgacc gtcttcctcg ccaatatctg ttatccgaac gatatgagga tggtctagat
     3301 ctgccatagc tctcgcttca cgctgaaaac gagctacagc tatcgggtcc gtctggtagt
     3361 tggtcctcag aaccttcact gccacttctt ccccatctaa gattaagtct ttggctaggt
     3421 agacatccgc catgcctcct cgaccaatct gtttgacaat ccgatagcgt ccggcaaaaa
     3481 tcttgccgat ttggatcatt ctgcatcctc ctcgttcata aaaacaaggg caaccgtaat
     3541 gttgtctaaa cctcctgcat tgttagcaaa acgaacaagt gtctccgttt tatctgctaa
     3601 aggaatatca ctggttacaa tatcacgaat ctcactgcct gaaatcatgt tggtcaagcc
     3661 gtcactattg agcaagagat agtcacctga ctcaaggata actgtcccaa aatcaggctg
     3721 aatttcatct ttttgcccaa tagactgggt gataatattt ttttgcggat gagcttctgc
     3781 ctcttctggt gtcaattgac cagccttgag caattcatta accaaggaat gatcgctcgt
     3841 caactgatgg tattcttctc cacgaatcaa gccgatacgc gaatcaccaa tatgagcata
     3901 gatagcctga ttatcaataa tagcaaggac ttccaaagta gttcccatgc ctctgtaagc
     3961 ttcatcctga ccaagctggt gaatcttttg attttcaatt tctaggtaat gggcgaacca
     4021 ttcacgcact tcattgactg tatcgatctg ggtatcaacc caagctacac ccaggtctgt
     4081 gaccgccatt tcactagcga tattccctgc acgatgacct cccatcccat cagctaaaat
     4141 aatcatggta cgtccagctc tattgacata gtggttgaca tagtcttggt tatttgttcg
     4201 tttctgacca acatctgtta ataatgaaat ttccatgtgt cagttccttc ctaatccgat
     4261 atcttgcgaa attgactgat gaagaatcca tcacttccat acaattcagg tgtaatgagg
     4321 atacagccgt ctttcatgat atccttacat tcatgttcta gttttacctg ctcgaactcg
     4381 ggatgactct ctaaaaaggc cttaacgact tgaaaattct cctctgagac gatagtgcag
     4441 gtgctataag ttattatacc acctttgcct agtatttgac aaacactacc taatatttct
     4501 aactgaattt cctgcaagga cgcgaaatct gccgtttctt tattgtattt gatatctggt
     4561 tttcggcgca aaagaccgat tcctgaacaa ggagcatcca ccaaaatctt atcaaaggaa
     4621 tcctggtcaa aaaactcatg cacctttctg gcatccaatt tttgagtttg aacccgatct
     4681 tcaactccca gacgttgggc attttcttga attaaatcca acttgtggtc gtacaagtcc
     4741 agagcagtaa cctgacctgt cgtaagataa gaggctatat gggctgtttt cccacctgga
     4801 gccgcacagg catcaagcac tcgctcatca ccttgtaaat caagcgtcgg agcaaccaac
     4861 tgactggact cgtcttggat ggtaatggct ccatccgcaa acaaattatg ccctgcaaaa
     4921 tgcccctgct ccttaaccag accagtggtt gctaaaaggg aattattcgc ctccaacaag
     4981 gcttggattt cctcttttcg acttaggtct gttacacgaa tactggcttt gtttcgcact
     5041 aacaggcttt caaagatggc ttttgctctc tcctctccgt attcttcctt gagtttggca
     5101 actagccaaa ctgggagaga ataggcaatg gagtcacgct tgttttttcg cttgatgcta
     5161 gcaatatctg gccagccttc acgcaagata cggcgaagga cagcgttgac caatttttca
     5221 ctgccttttt tacggagttt ggccaattcc actgcttcat tgaccacagc atgatctgga
     5281 atcttgtcca aatagcggag ttggtaggca ctcatgagaa gaaggacata gagccagctg
     5341 tctaactggt ctctgtcttc gataaagtgg gataggtacc attccagagt cagtttacgg
     5401 gctaccgttc catagactag ctcggtcact aagcccttgt ctgctgcaga gagctgactc
     5461 cccttgaggt gtttatttaa ggcaatattt gaatatgctt gattcacaaa aacatcctct
     5521 agcactgcta gagctaaact tctagccgtt tctactttag tcaccaaatc gttctcctac
     5581 agtcaatgta cgtccaactc cattgaggaa ggaagcaatg tccatcttag gcttaccagc
     5641 tggctgcact tgtttgaggg atagagcccc ttcagccgtt gcgacaatca attctttctt
     5701 gccgatagag agaatctcac ctggatttcc ctgaccttct actggtaggg cttcataaat
     5761 cttaaagcgg tcgcccttaa ggaaagtatg ggcaacaggc caggggttca ttccacgaat
     5821 ttggttaaag agttgacgat tggttttgtt ccagtccagt ttttcttcct ctggctttat
     5881 atttggagag aaggtaacct gactcgtatc ctgcggttca ggtttgatat caccagcaat
     5941 ataggcaggc agagtgtcca aaagcaaatc acgaccaact agcgccaatt tttcaaacaa
     6001 ggtgccaaca ttgtcctcat ctgtgatcgg aatgctgcga cgagaaatca tatctcctgc
     6061 atccatttcc ttaaccattt ccatgatggt cacaccagct tcctcatccc cttgaatcaa
     6121 ggcataatgg ataggcgcac caccacggtg tctaggaagg agggaggcat gaacgttgac
     6181 agcaaagtcc atgctatcaa ggagtttgct tgggagaaac tgcccaaaag cagcagtcac
     6241 aattccatct gctcctagct tcataagttc ttccatctct ggacttccag ataatttttc
     6301 aggttggtag atagatagtc ctgcttcctt ggcagcctgc ttgactgggg tttcttggat
     6361 aactttttta cgaccaacag cacggtctgg ctgggtcaca acggctagaa tttcgtaacg
     6421 gtcatctgtc aaaagtcctt ttaagactgt tgctgaaaag tcgggggtcc ccataaagat
     6481 tagttttgtc atatcttctc cttcttataa aaattgctgc ggctcatggt caatgctgag
     6541 acggagctca ctattttccc gttcttgagt caaggccaag acctggttga gggtcggccc
     6601 cagctcatct tctaaacggt atttaattaa aatctggtaa tgatagaggt tgtgggtacg
     6661 ggcaatcggt tttggcgttg gccccagaat gggactggtc tctgacaagc ctgaccgcaa
     6721 aatgttcatg acttcatagg cacgtttgaa aacctcttct tctttcttgt gagaaagggt
     6781 aataccaatc gtgaaatagt aaggtggata gccgagttgt cgtctgattc ccatttcata
     6841 ggcataaaag ccttcgtaat cttgatcctt ggcaaatcga atagcatagt gctgcgaatt
     6901 gtaggactgt atcaagactt gacctgcttt ttcagcccga cctgctcgac ctgccacctg
     6961 agtcaagagc tggaaggttc tctcagaaga acggaaatca ggcagattca aggccgtatc
     7021 cgcatttaga actccgacta gggtaacatt gggaaaatcc aaaccctttg caatcatctg
     7081 agtaccaagt aaaatatccg cttcccctcg cccaaactgg tcaagcaagg cttggtgact
     7141 gcctttcttt cgagtcgtat ccacatccat cctcaaaatg cgagcttggg gaaagagttc
     7201 tgctagctca tcataagcct tctgagttcc cgtcccatag taacgaatac tgcggctctt
     7261 acagttagga cagacctgag gaatatcctt cgagaaacca caataatggc agttcatagt
     7321 cttggtatcc atatgcaagg tcagagaaat atcgcagttg ggacaagtat ccaccgtccc
     7381 acactcccga cacatgacaa agctagaata accacggcga ttgagcatga gaaccacctg
     7441 ctctttttta accagacggt cttggatagc ctctagcaaa ggaggcgtaa agtttgacgt
     7501 ctcattttgt ccgatatagt ctcgaaagtc aatcacttga acctcaggga ttgtagccaa
     7561 aggattggca cgttgggtta gacgtaagtg ttgatagacg cctttgccag cacgtgcccg
     7621 gctctctaag ctcggcgttg cagatccaag taccagagtt gcttgattat actgagcccg
     7681 taaaatagct acctctctgg catggtaacg gggattgctg tcctgcttat aagccgcttc
     7741 atgctcttca tcaataatca tgacacccag atttttcaga ggagcaaaga tagcagatct
     7801 ggcaccaaca acaacttggg catcgccacg ctccaccttg cgccattcat catacttttc
     7861 gccattggat aatcctgagt gaagaatggc taccttgtcc ccaaaacgtg ctataaaacg
     7921 ctcggtcatc tgaggagtca aggaaatctc aggtaccagc aaaatagctg tcttaccctt
     7981 atccagggca ccttggataa tctgcaagta aacctcggtc ttcccacttc ctgtaatccc
     8041 ttgaagtaga aagggaggtt gagaactgcc aatagaactc acaaccgcat cacgcgcctg
     8101 tctttgttct ggatttaact ccaaaggtct acttgcttca attccttcaa aataagcagc
     8161 cgagcgttga acttcctttt ggactatggt aacagtacct tgatccacaa agaagttgac
     8221 ttgctctcgc gagtaggact ctaacaagct agccaaggaa gcgctctctg gatgagacag
     8281 cagataatct ctcagttcca actttttctt ggcacgtgta gaaatctcaa caccttctaa
     8341 ttgggcatgg tcaacctcat accaagactg ggtcttgacc ttcttttgat cgactgcctg
     8401 atattccaga ccaagcaggc cttttctagt caaacgcatc atttcagctt gcttggcaag
     8461 gtctagtgaa gaaaaggcta gcgaatcttc tgaaccaaac aggcgtactc gttcttcctg
     8521 actcaagcct tccagaggat agagaatctt gtcatagcta gaattcagaa accctggaag
     8581 catggacttg aggatagaga ttttgtagga gaagacagat ttgcgtaact cctcagccag
     8641 ccagagttgt tctggcgtga gaacaggaga aaaatccagc acctctgcaa tatcttttaa
     8701 atcttgctcc atctcttctc catctgattg ggacttcaaa ccaagaacaa tcccttgaat
     8761 caggcgatta cccttaccaa aaggcacatg aacccgcatc ccaacttcca gcattccctc
     8821 aaattcctcc ggaatcctgt aactataggg ctggtccgtc tgcatcaagg gcacatctac
     8881 gataatctta gctagggcca tcttctcacc tcctccttgt cagtacattc ttgcaataga
     8941 aaaaataaga ttgagtcccc ccaaccttaa attttttcac catcttcttt ttctttagca
     9001 atttgctctt tgattttctt ttcttcttct tctttgcggc gtttttcttc ttcgatacgg
     9061 cgacgcactg cttcacgttt tccttctgga tctgggtgaa ttgtaacgtt tcctgattcg
     9121 atttcttcta aagcgcgaag agttgatttt tcagacttga aaccttgagt tgctggggca
     9181 cctgcttcca attcgtgggc acgttttgct tccaagatta cgagtgaata ttttgaagga
     9241 accttgtcga gcaaggtatc aatagagggt tttaacatca tttgcttgta cctattttct
     9301 aaattttatc gggtagttgg agattttggt aacatctcct gatagtgacc aatgacacga
     9361 tccacacaga agtgttctgc ttcaatcaca catttgacac gttcagcagc taggggcacc
     9421 tgatcgttga caatcgcata atcatactca cgcatgaggg caatttcttc cttggccttt
     9481 tcgattcgtt gggcaatcac ttctgcacta tctgttccac gacctaccaa gcgatcttgc
     9541 aattcatcca aatctggtgg tgtcaggaag ataaagacag catctggaac ctttttcttg
     9601 acctgaagag caccctgaac ttcaatttca aggaaaacat cgattccctt gtccaaggtt
     9661 tcattgacat aggtcagagg agttccatag tagttgccga catattctgc gtattccaac
     9721 atctgtcctt gacgaatcag ctcttcaaat tcttcacgag tacggaagaa atagtcaaca
     9781 ccgtccactt ctccaggacg ttgtgcgcgt gtcgtcatcg atacagaata ttgaaattgg
     9841 ttttcagaac tctcaaaaat ctctcttcta accgttcctt ttccaacccc tgaaggacca
     9901 gaaaaaacga ttagtaagcc tcggtctgcc attgtgtctc cttttagtca atctgtgaaa
     9961 taacatttct ctagaataat ggcaaaaagc cagattatcc tttacagtct ttctatctag
    10021 tgtaacaaaa aagcagtaat ttttcaactg ctctttctta tttatttagc ataatctact
    10081 gcacgaagct cgcgaatcac ggttaccttg atatttcctg gataatcgag attgttttca
    10141 attttcttac gaactttgtg agccaagatt gtgactttgt cgtccttgat ttttcctgga
    10201 ttgaccatga tacgaatttc acgtcctgct tgaagggcaa agctagtttg cactccttca
    10261 aagccgttag caatttcttc caaatcatgg agacgcttga tgtagctttc aagagactca
    10321 ctacgagcac ctggacgggc tgcgctcaag gcatctgctg cagcgacgat aactgctatc
    10381 acgctctcag cttcaacatc tccgtggtga ctagcaatcg tattcaccac aactgggggt
    10441 tccttgtact tacgggccaa ttccataccg atttcaacgt ggctaccttc aacctcatgg
    10501 tcaatggctt tcccgatatc gtgaaggaat ccagcacgac gggcaagagc cgcattttca
    10561 ccaagttcgc tcgccatgat accagccaac ttagcaacct caatcgaatg gcgcaaaaca
    10621 ttttgtccat atgaagtacg gaactgcaaa cgtcccataa tcttcatcaa gtctggatga
    10681 aggtttggcg caccaatttc ataggcagca gcctcaccgt attcacgaat cttattgtca
    10741 atctcttgac ggtttttctc aaccaactct tcgatacgag ctggatgtat acgaccatct
    10801 ttgagcaaca tttccatagt catacgggca atctcacgac gaatcggatc aaatcctgac
    10861 aaggtcacca cttctggtgt atcgtcgata atcacatcga cccctgtcaa actttcaaag
    10921 gtacgaatgt tacgaccttc acgaccaata atgcgtccct tcatagtatc gtctggcaga
    10981 tgaactgttg agtttgttga ctccgctaca tattcaccag cgatacgttg catagcttga
    11041 accaagatgt ccttggccat tttgtcagaa cgttccttga cctcttgctc agcttcgcga
    11101 atgcgactgg caatctccct ggtcaagttt tcctctgtct gagccaagat aatatctcgt
    11161 gcttctgcct gagacagagc accaatacgc tctagttctg cttctttttg tctttcgact
    11221 tcctctaatt gctcttcacg cgcatcaagg tttttcgctc tatcagaaat actttgttct
    11281 ttttgttcaa gtgtttgttc tttactcgtc aaattgtcgt ccttacggtc aaggctagta
    11341 gctctctctg tcaaacgact ttcgatttgt ttgagttctt gacgttctga tttgaattca
    11401 gcgtccactt cttcacggta ttttctggct tcttctttgg cctccaatag tgcttctttt
    11461 ttaagagact tgctttcacg tttggcttca ttaacaagta aatccgcttc acgctcagct
    11521 tgtccacgta aattagttgc ttcttgttca gcatttaaaa gcatcaactc tgcagcttcc
    11581 tgagatgatt tcatcttagc tgagatgctg acatatccaa tgactaaacc aatgatgacg
    11641 gcaaaaacag caatcgcaag cgacatgatt tccatgtttt tacctcattt tattgttatt
    11701 ccgaatgaca tacattcttt tacattctac cataaaaaag tgattttcac aaacctaaaa
    11761 tagaatatgt tttggggaat ttggaacaca tttaccaaaa taaacttgtt gtttagaaat
    11821 agtagtttag tagagacttg agaaaaagcc tactttgcag tagacttagt aatgatcttt
    11881 aaaggacaag aaagccacgc tatctccatc catcatataa atcaagcgat tttctgcatc
    11941 aatacgccgt gaccaggctc cttggtaatc atatttgagt ggctctggtt ttcctattcc
    12001 tgtaaatgga tcgcgttgaa tatccttgat taatttattg attcttttta gtgttttttt
    12061 atcttggttt tgtcagtagc aataatctgc ccaggcatct tctgtaaact tgagtagcat
    12121 tcatcactcc tcaatcacat ggacctgagt gcttccagca cggacttgag ccattcctcg
    12181 caaaaccttg tctgataact ccttattttg ggcgattctc agggtttcct gaatactgtc
    12241 ccattcgctc tttgaaagaa ctacaatgtc ctcatctgga tttttattga ccaccgtcaa
    12301 aggctcaaat tcatcattta ccttcttcat gtagtccttt aaatgatttc ggaatgttga
    12361 gtaaaggact gcttccataa ccatacctcg ttttagctct tttccactat tatacacgaa
    12421 aagaaagaaa ttgtcaggaa cttgtacaag attttctttt ctatctatt
//
LOCUS       DAVBCW010000053        12413 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVBCW010000053 DAVBCW010000000
VERSION     DAVBCW010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12413
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.053"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            <1..208
                     /locus_tag="VQ514_001278"
     CDS             <1..208
                     /locus_tag="VQ514_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2500032.1"
                     /translation="DNARFHRMGKLELLCEEFGHKLLPLPPYSPEYNPIEKTWAHIKK
                     HLKKVLPSCCNTFYEALLSCSCFN"
     gene            complement(415..2673)
                     /locus_tag="VQ514_001279"
     CDS             complement(415..2673)
                     /locus_tag="VQ514_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET2500033.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(2902..3132)
                     /locus_tag="VQ514_001280"
     CDS             complement(2902..3132)
                     /locus_tag="VQ514_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HET2500034.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            3322..4005
                     /locus_tag="VQ514_001281"
     CDS             3322..4005
                     /locus_tag="VQ514_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2500035.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSEIVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            4005..4739
                     /locus_tag="VQ514_001282"
     CDS             4005..4739
                     /locus_tag="VQ514_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2500036.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKVIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            4893..5750
                     /locus_tag="VQ514_001283"
     CDS             4893..5750
                     /locus_tag="VQ514_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HET2500037.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            5892..6764
                     /locus_tag="VQ514_001284"
     CDS             5892..6764
                     /locus_tag="VQ514_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HET2500038.1"
                     /translation="MKVINQTLLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLMTVGTDRENIPALHSHLPEPMAFSLQDQQLLKEQLEKAEVILLGPGL
                     GDNAFGEDLVKQVFAGLKQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQERVSKDKLV"
     gene            7046..7729
                     /gene="rpiA"
                     /locus_tag="VQ514_001285"
     CDS             7046..7729
                     /gene="rpiA"
                     /locus_tag="VQ514_001285"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HET2500039.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            7743..8954
                     /locus_tag="VQ514_001286"
     CDS             7743..8954
                     /locus_tag="VQ514_001286"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HET2500040.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIRESNKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            8956..9498
                     /locus_tag="VQ514_001287"
     CDS             8956..9498
                     /locus_tag="VQ514_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HET2500041.1"
                     /translation="MTRYALLVRGINVGGKNKVVMAELRQELTNLGLEKVESYINSGN
                     IFFTSIDSKAQLVEKLETFFAVHYPFIQSFSLLSLEDFEAELENLPAWWSRDLARKDF
                     LFYTEGLDVDQVIATVESLELKDEVLYFGKLGIFWGKFSEESYSKTAYHKYLLKVPFY
                     RHITIRNAKTFDKIGQMLKK"
     gene            9514..10323
                     /locus_tag="VQ514_001288"
     CDS             9514..10323
                     /locus_tag="VQ514_001288"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HET2500042.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            10799..11779
                     /locus_tag="VQ514_001289"
                     /pseudo
     CDS             10799..11779
                     /locus_tag="VQ514_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855254.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
     gene            11804..12316
                     /locus_tag="VQ514_001290"
     CDS             11804..12316
                     /locus_tag="VQ514_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HET2500043.1"
                     /translation="MSAQITINHKKARYVRIELEGYNALSLAEVEVFRFIATNAETAT
                     QVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGNGR
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPISAQPHRKITHWGTTL
                     NSKVSDDDVL"
BASE COUNT         3668 a         2272 c         2805 g         3668 t
ORIGIN      
        1 ggataatgca agattccata gaatggggaa gctagaactt ttatgcgaag agtttgggca
       61 taaactttta cctcttcctc cctactcacc tgagtacaat cctattgaga aaacatgggc
      121 tcatatcaaa aagcacctca aaaaggtatt accaagttgt tgcaatacct tttacgaggc
      181 tcttttgtcc tgctcttgtt tcaattgact atagcagatt gaaataagat gagaacaaat
      241 cgattgggaa agtaaaatta atttctataa atgttttagc aattgtttcg tactatttca
      301 gattcagtct actatatatc ataatggaca aagggcaagc tgtcaagaaa ttacagttat
      361 tttagtaaaa aaagaagcag aaaaatttaa ttttctactc cttttctata ggatttattt
      421 ttctttttca gaacttttaa cttcagcttt tttggcagat ttaatctgaa tgtttccctt
      481 gctagtcata actgctttga gatctttttc gcttggattt tcaaggtagt agtcagtgat
      541 tgtgtcctca atatagtctt gaatagtccg acgaagtggg cgtgctccca tttttggatc
      601 ataacctagg tcaaccaact tttccttgac cttgtcagtt acatccaaac gaatgttgtt
      661 gctagagagg cgcttgttaa catctgctag catgagctcg acaatctgaa ggaggttatc
      721 cttgctgaga gccttaaatt cgataatgcc atcaaaacgg ttcataaact ctgggctaaa
      781 gaagttaccg agttcaccga ggacagaatt ggtacgtcct tctctagcag caccaaatcc
      841 aacgctagct tcggtctttc ctgtacctgc atttgaggtc atgataatga tggcatcctt
      901 gaagctaacg gtgcgtcctt gcccgtctgt caaacgacca tcgtccaaga cttgaagaaa
      961 catgtgcata acatctgggt gagctttttc cacttcatcg agaaggatga gagaatatgg
     1021 attgtggcga actttttcag ttaattgacc agcctcatca tagccaacat aacctggagg
     1081 agcgccgacc aacttagcca cactatgttt ttccatgtat tcactcatat caaagcgaat
     1141 catactatca gcagaaccaa aaagttcgat agccagttgt ttggaaagtt ctgtcttacc
     1201 gacaccagtt ggcccaacga agaggaagct tccgattggg cggttagggg taccaagtcc
     1261 gacacgatta cggcgaatag ccttggcaat cttatcgact gcatcatctt ggccaataac
     1321 atgagacttg agatcttcgg ctagatggat gagttgagat tgttctttct ctttcaaatc
     1381 accaacaggg atattggttt tctgctcgat aatgtgctca atagttttct cgctgatgat
     1441 aggagtatcc tggtctgtga tctttttctt ttgcatttcc ttatacttgg caatctggtc
     1501 gcggaagtag gccgccttct caaaatcttc ttctcgtgta gcttgagact tgagattttc
     1561 agcctcaatc aagcgctgat caattacttt aggatccaca aaattcaagg tcaagttcat
     1621 cttagaacca gcttcatcta ggaggtcaat ggccttgtca ggcaagaagc gatcttggat
     1681 gtagcgattg gaaagagttg cagctgcttc aatcgcagca tctgtatatt gaacgtggtg
     1741 gtaatcttcg tatttctttt gaatcccttt gagaatagtg attgtttcgt ccaccgttgg
     1801 ttcatcgact ttaacaggct gcatacgacg ctcgagggca gcatcctttt caatgatacg
     1861 gtattcattg agggtagtag caccgactag ttgcagttct ccacgagcaa gggctggctt
     1921 gaggatattt cctgcgtcca tattaccatc actcgcagaa ccagcaccaa caatttcatg
     1981 gatttcatcg ataaagagga tgatgtcttc acgtttgcga atttcttcca tgagtttttg
     2041 catgcgttct tcaaattgtc ctcgaatccc cgttccttga actaagctaa ccacatccag
     2101 acggatgact tgtttacctt ggagtttatg tggcacatcg ccatcgacaa ttttctgagc
     2161 tagaccttcg acaacggccg tttttccgac accaggttca ccgataagga caggattatt
     2221 cttggttcta cgattgagaa tctcgatgac acggataatc tcatcgtcgc gcccaataac
     2281 ggggtcaatg tctccacgac gggcaatttc agttacatta ataccaaatt cttccagcag
     2341 gcctttttct tggctaggtg gcggagtttg agcagatcca cgattttggg aaccataacc
     2401 gccgtttcca ccgtaacctc cacctgattg ggttggggga ataggaggag tattgctaga
     2461 aggtctgaaa ttgtttagat cattgaagaa atcaccaaag ggatcgaagt cacgattgtt
     2521 cagatccgtc atacctttga agaggctatt gttaggatct gttttgataa tcttatagca
     2581 gttttgacag aggtcaattt gtttttgttt tccattgaga ttggtgtaaa gatgaattgt
     2641 tgagtcgtta attttacagt tttgacaaag catacatcta cctcatttct ttctttagcc
     2701 tgacttgttt aaaggtcaaa aatagtcaaa ttctatactc tcattataac aggattgggg
     2761 tgtaaagcaa gtaaaaagat tgaagctttg agaagttgtt acaatgaaaa tttttgtgaa
     2821 tttggactat aaaaaaatcc tatttgtgag acaaaaagga ttttacttag acatgcaggg
     2881 acaatcccag cttgttttag attagtagag aagttcgtaa atttccattg cgattaagtc
     2941 aatgctatca aacgtatacg tttcgcgatc ttctacgtca cgaagggtaa agattgggcc
     3001 gttttcttcg tcgtggctgt acgcaacttc tgcaacaaca actccttcgc gttcaaaatt
     3061 acgttttaaa tttccgccat cgtttgccat tgcttcaagg cggttgatga ttctaaccaa
     3121 atgtgattcc attttgattc tccttcattt cttatcgtta ctattttacc ataaaggagg
     3181 ccaacttgac tagaaaaaac taagatttct cgctaattct gctagtttaa agatttttaa
     3241 ataaattgga taaaaaattt aaattttaat tcaaaacatg ttataatcat acagtattct
     3301 attttagaaa gcagtgtgac tatgaatttt tcttttttac ctaagtattt accttatttt
     3361 aactatgggg ctgttgtgac gattcttatt tctatctgtg ttatcttttt gggaactatt
     3421 ttgggtgttg tcttggcttt tgggcaacgt tcaaagttta aaccgcttgt ttggttggcc
     3481 aacttgtacg tttggatttt ccgtgggaca ccgatgatgg ttcaaattat gattgccttt
     3541 gctcttatgc atatcaatgc tccgactatt cagattggaa ttttaggtgt tgatttttcg
     3601 cgtctgattc cagggatttt gattatctct atgaatagtg gtgcttatgt ttcggagatt
     3661 gttcgtgccg gaatcaatgc ggttccaaaa ggtcagctag aggcggctta ttcgctaggg
     3721 attcgtccta aaaatgcgat gcgttatgtg attttgccac aagcagtcaa aaatatcttg
     3781 ccagcattgg ggaacgaatt tatcaccatt atcaaggaca gctccctctt atcagctatt
     3841 ggggtcatgg agttgtggaa tggggctaca acagtttcta caacaaccta tctaccttta
     3901 acaccacttt tatttgcagc attttactac ttgattatga cctctattct gacagtagcc
     3961 ttgaaagctt ttgaaaaaca tatgggacaa ggagataaga aataatgaca gaaaccttga
     4021 taaaaattga aaatttacat aaatcttttg gaaagaatga agtattgaag ggcatcaacc
     4081 tcgagattaa aagaggagaa gttgtcgtta tcatcggtcc ttcagggagt gggaaatcta
     4141 ccttgcttcg ctctatgaat ttgttggagg aagcaaccaa ggggaaggtt atctttgagg
     4201 gagtcgatat tacggacaag aagaatgacc tgtttgccat gcgtgagaag atgggcatgg
     4261 ttttccaaca atttaacctc tttcctaata tgactgtgat ggaaaatatt accttgtcac
     4321 ctatcaagac taaaggtgaa agcagggaag ttgctgagaa gagagcccaa gagcttttgg
     4381 aaaaagttgg tttgccagat aaggcagacg cttatccaca gagtctgtcg ggtgggcaac
     4441 aacaacggat tgccatcgcg cgtgggttgg ctatggaacc agatgttttg ctctttgacg
     4501 aaccaacttc agccttggat cctgaaatgg taggtgaggt attggctgtt atgcaagacc
     4561 ttgccaagtc agggatgact atggttatcg taacacatga gatgggattt gcccgtgagg
     4621 tggcagatcg tgttatcttt atggcagacg gtgtggttgt tgaagatggg acacctgagc
     4681 agatttttga acaaacccaa gaacaacgga ctaaagattt cttgagtaag gttttataat
     4741 ctattttaaa atttcaagac aagattagtg aaaagttcaa ccagagcttt ttcttatagt
     4801 ttgaaactat aggataacct aggaaagaag tgttagaggg cactagcagt ttttggtata
     4861 atggaagata tttgaaagaa aagagaagtg atatgacaca gattattgat gggaaagctt
     4921 tagcggccaa attgcagggg cagttggctg aaaagactgc aaaattaaag gaggaaacag
     4981 ctctagtgcc tggtttggta gtgattttgg ttggggataa tccagccagc caagtctacg
     5041 ttcgcaacaa ggagaggtca gctcttgcgg ctggttcccg tagcgaagta gtgcgagttc
     5101 cagagaccat tactcaagag gaattgttag acctgattgc taaatataat caggatccag
     5161 cttggcatgg gattttggtc cagttgccat taccaaaaca tatcgatgaa gaggcggttt
     5221 tattagccat tgacccagaa aaggatgtgg atggtttcca tcccctaaac atgggacgtc
     5281 tttggtctgg acatccagtc atgattccct cgacacctgc aggaattatg gaaatgtttc
     5341 atgaatatgg gattgacttg gaaggtaaaa atgcggtcgt aatcggtcgt tcaaatatcg
     5401 ttggaaaacc tatggctcag cttcttttgg ctaaaaatgc gacagtaacc ttgacccact
     5461 cacgaactca taatcttgcc aaggtggctg ctaaagcaga tattcttgta gtcgcaatcg
     5521 gccgcgccaa gtttgtgact gctgactttg tcaaaccagg tgcggttgtc attgacgttg
     5581 ggatgaatcg agatgaaaat gggaagctct gtggagatgt tgattatgag gcagttgcac
     5641 cacttgctag ccatatcacg ccagtccctg gaggtgtcgg tcctatgacc attaccatgc
     5701 tgatggagca aacttatcaa gcagcacttc ggacattgga tagaaaataa gagaagcatt
     5761 cgattaatag agtgtatttt ctatagctat atctaaaatg gcagaaatga atattaaatt
     5821 ttagatataa gattacaaaa ggaggtctgc gtctcctttt tattgtataa taaaagtgag
     5881 aggaaaaaag gatgaaagtg attaatcaaa ccttattaga aaaagttatt attgaacgtt
     5941 ctcgttcaag tcataaagga gattatggtc gtctgctgct gcttggtggt acttatcctt
     6001 atggaggtgc catcatcatg gctgctttag cagctgtaaa aagcggtgca ggattgatga
     6061 ccgttggaac ggacagggaa aatatcccgg ctctgcacag ccatttacct gagcctatgg
     6121 ccttttctct tcaagaccag caattgttaa aagagcaatt ggagaaggca gaagttatct
     6181 tgctggggcc tggtttagga gacaatgctt ttggagaaga tttagttaaa caggtctttg
     6241 ctggcttaaa acagaatcag attttgattg tagacggagg tgccttgacc attcttgcta
     6301 ggacaagttt gtcatttccc tcaagccagc ttatcctaac tcctcaccaa aaagaatggg
     6361 aaaaattatc tggaattacg attgaaaagc aaaaggaaga tgcaacggct agtgttctga
     6421 cttccttccc tcaaggaaca attttggtgg agaaaggccc agctactcgt atttgggaag
     6481 ttggccaatc tgattattac cagttacagg ttggtggtcc ttatcaggcg actgggggaa
     6541 tgggagatac tctggctggg atgatcgcag gatttgtagg ccaattccga caggccagtc
     6601 tctacgagcg tgtggcagta gcgacccatc ttcattcagc catagcccaa gaactatctc
     6661 aagaaaatta tgtggtctta ccgacggaaa tcagcaggta tcttcccaaa ataatgaaaa
     6721 ttatttgtca acaagagagg gtaagcaaag ataaacttgt ttaaaataga cagataaatc
     6781 tacttccttc tcttaattta tgattcttga aatgcgcctg ctttaaggct aaactagacg
     6841 tatcaaagtt aagagtttgt ttttgtaact ggaaagagat gaacaaatca aatacataaa
     6901 agataaactc atttttattc gttttgtaaa aataaaatgc atatttttaa agaaagattg
     6961 atgaaaaatg tagatttgat gtttttactt gaataaaaag cggtttcata gtaaaataag
     7021 aactgagaat gatggaggta aatcggtgga aaatctgaag aaaatggcag gtatcaaggc
     7081 tgctgagttc gtgagcgatg gaatggtcgt tggacttgga acaggctcga ctgcctatta
     7141 ttttgtcgaa gaaatcggtc gtcgtatcaa ggaagaagga ttgcagatta cagctgtaac
     7201 aacttctagt gtgaccacta aacaggcaga aggccttaat atcccgctca agtctattga
     7261 ccaagtagac tttgtcgatg tgacggttga tggcgcggat gaagtggata gtcagtttaa
     7321 tggaatcaaa ggcggtggtg gtgcccttct catggaaaag gtggtcgcaa caccatcaaa
     7381 agaatacatt tgggtggtgg atgaaagcaa gctggtcgaa aaactaggtg cttttaaatt
     7441 gccagtagaa gtggttcagt atggtgcaga gcaggtcttt cgtcattttg aacgagctgg
     7501 ctacaaacca agtttccgtg aaaaagacgg tcaacgtttt gtgacggata tgcagaattt
     7561 tatcattgac ctcgccttgg atgtcattga aaatccaatt gcttttggac aagaattgga
     7621 ccatgtcgtt ggtgttgtgg agcatggttt attcaaccaa atggtggata aggtaatcgt
     7681 tgctggacga gatggagttc agatttcaac ttcaaaaaaa ggaaaataga agggggcata
     7741 agatgtctaa atttaatcgt attcatttgg tggtactgga ttctgtagga atcggtgcag
     7801 caccagatgc taataacttt gtcaatgcag gggttccaga tggagcttct gacacactgg
     7861 gacacatttc aaaaacagtt ggtttgaatg tcccaaacat ggctaaaata ggtcttggaa
     7921 atattcctcg tgaaactcct cttaagactg tagcagctga aagcaatcca actggatatg
     7981 caacaaaatt agaggaagta tctcttggta aggatactat gactggacac tgggaaatca
     8041 tgggactcaa cattactgag cctttcgata ctttctggaa cggattccca gaagaaatcc
     8101 tgacaaaaat cgaagaattc tcaggacgca aggttattcg tgaatccaac aaaccttatt
     8161 caggaacggc tgttatcgat gattttggac cacgtcagat ggaaactgga gagttgatta
     8221 tctatacttc agctgaccct gttttgcaga ttgctgccca cgaagacatt attcctttgg
     8281 atgaattgta ccgtatctgt gaatacgctc gttcgattac ccttgagcgt cctgcccttc
     8341 ttggtcgcat cattgctcgc ccttatgtag gtgaaccagg taacttcact cgtacggcaa
     8401 accgtcgtga cttggctgta tctccatttt ccccaactgt tttggataaa ttgaatgagg
     8461 ctggtatcga tacttatgct gtgggtaaaa tcaacgatat ctttaacggt gctggtatca
     8521 accatgacat gggtcacaac aagtcaaata gtcatggaat tgatacacta ttgaagacta
     8581 tgggacttgc tgagtttgaa aaaggattct cattcacaaa cctagttgac tttgatgccc
     8641 tttacggcca tcgtcgtaat gctcacggtt accgtgattg cttgcatgag tttgatgaac
     8701 gcttacctga aattatcgca gctatgagag agaatgacct tctcttgatt actgcggacc
     8761 atggaaatga cccaacgtat gcaggaacgg atcacactcg ggaatatatt ccattgttgg
     8821 cctatagccc tgcctttaaa ggaaatggtc tcattccagt aggacatttt gcagatattt
     8881 cagcgactgt tgccgataac tttggtgtgg aaactgctat gattggggaa agtttcttag
     8941 ataaattggt ataagatgac gcgctatgct ttgctggtga gaggtatcaa tgttggtggt
     9001 aagaataagg tcgtcatggc ggagcttcgt caagaattga caaacttggg actggaaaag
     9061 gttgagagct acatcaatag tggcaatatt ttctttactt cgatagattc caaagcccaa
     9121 ttggttgaaa agctagagac tttctttgca gtccattatc catttattca gagcttttct
     9181 ttactgagtc tagaggactt tgaggcggaa cttgaaaatc taccagcttg gtggagcaga
     9241 gacttggcac gaaaagattt tctcttttac actgagggtt tggatgtgga ccaagtcatc
     9301 gcgacagttg aaagtttaga gctgaaagat gaagtgcttt attttggaaa acttgggatt
     9361 ttctggggga aattttctga agaatcctat tctaagactg cctatcataa gtacttgctg
     9421 aaggtgcctt tctaccgcca cattactatt cgtaatgcta aaacctttga caaaattggt
     9481 caaatgctaa aaaaataata aaggagacac acaatgacat ttttaaacaa aatccatgaa
     9541 actgctactt tcctgaaaga aaagggaatt gcagcccctg agttcggtct aatccttgga
     9601 tcaggacttg gagaattggc agaagaaatc gaaaatccag ttgtagtaga ctatgctgag
     9661 attccaaact gggggcgttc aacagtagtc ggtcatgctg gtaaattggt atatggtgaa
     9721 ctggcaggtc gcaaggtctt ggctcttcaa gggcgtttcc atttctatga agggaatcct
     9781 ctggaagtgg tgactttccc agttcgtgtg atgaaagttc ttggatgtga aggtgttatt
     9841 gtaaccaatg cagctggcgg tatcggattt ggtcctggta ccttgatggc tatctcagac
     9901 catatcaaca tgacggggca aaatccattg atgggtgaaa acttggatga ctttggccca
     9961 cgtttcccag atatgtctag ggcctacaca ccagaatacc gtgccactgc ccatgaagtg
    10021 gctaaaaaac ttaatatcaa gcttgatgaa ggtgtctata tcggagttac tggtccgact
    10081 tatgaaacac cagcagaaat tcgttcctat aagacactgg gagcagatgc agttggtatg
    10141 tctacggttc ctgaagttat cgtggcagcc cactctggct tgaaagttct gggaatttca
    10201 tgtatcacta actttgcggc cggtttccaa gaagaactca atcacgaaga agttgtagaa
    10261 gtgactgaac gtgttaaagg tgatttcaaa ggcttgctta aagcgattct tgctgaattg
    10321 taagaaaaaa gatttaaaag ggggagtgcc tctgtttttt caggattgac tgcctatccg
    10381 gattaaagaa gaaacagagg aatactatga gcttcttcct gctcttataa ctgaaagaag
    10441 cggaagaata ggtatgtctg atctgatagc cagcattgtg aaagacaaga ttctaggata
    10501 ctagcattag cttcctagcc aagcagacta gtatgataag gagagatgag aatgaattga
    10561 ctttctgaat ttctcagtct tatcatatat agcacaatga gatttcgctt gagtctgctt
    10621 gtaaataaac gaaaagaaag ataagaaata atgaaaattg gtcaacgaat tatgcgcttt
    10681 ggcataaaaa attaagtatc ggagttgtat ctgttgtagt cggctttgat ttctagctcc
    10741 agctggaatt tcagccaatg aagtaaagca agatgtaaca tctgaagtgg taataggtgt
    10801 gctagattct aaggaggaat tgaaagagtc agaaaatgat gctccaaaac tagaaactcc
    10861 tcttagagag gagccaagac tagctcctca aacgcttccg gaagcaagtg aagttcttga
    10921 aaacaaaagg gaagagtcaa aagtagagat aacataacca gctcaagcgg atgatatccg
    10981 caaggttgtt ggggaattag ccaaggatat aagtattact aagttgtata tgacaggtca
    11041 ttctcttgga tgttacctag ctcagattgc agcggttgaa gcttaccaaa aatatcctga
    11101 tttttataac catgtattga ggaaagtgac aactttcagt gctcctaaag tgattacttc
    11161 cagaactgtt tggaatgcta agaatggttt ctgggatgtt ggtttggaaa gtcgtaaatt
    11221 agctgttagt ggaaaaatta agcattatgt ggttgataat gacaatgttg tgactccctt
    11281 gattcataat aatcgtgata ttgttacatt tacaggtaat tcacgcttta aacaccgttc
    11341 tcgtggctat tttgaaagtc caatgaatga tattcctaac tttaatattg gtaaacaagc
    11401 taccttggat aaacatggtt atcgtgatcc gaaattggat aaagtgcgat tctttaagaa
    11461 acaggctctg cctcgatctt ctagtcaacc aagcgctgaa ccaatggaaa atattgcctt
    11521 aggaaaacag gttactcaaa gttcgacagc tttcggagga gatgctagaa gagctgtgga
    11581 tggcaaagtc gatggtaact atggtcacaa ttctgtcact catacaaact tccaatctaa
    11641 gccttggtgg caagtagatt tggctaaaga agaaaccatt cgccaaatca atatttacaa
    11701 ccgaacagac actgcccagg atagattggc aaactttgat gtcattcttt tagacagttc
    11761 tggtaaagaa attgagtgaa aacgtataat atctcctaaa gatgtgtcag cacaaattac
    11821 gattaaccat aaaaaagcgc gctatgttcg gattgagcta gaaggctata atgccctcag
    11881 tcttgcagaa gttgaagttt tccgctttat agctacgaat gctgaaacgg cgacacaagt
    11941 ttctaagcca gttcaaccaa tcagtcagac tcctgtgaaa gataaaacat tgacaattca
    12001 acacagtgga gcttacattg cccgctactc cataacttgg gaagaagttc cagtagataa
    12061 agatggaaac caagttgttc gtagtcattc ttgggaagga aacggtcgca accagactgc
    12121 gggttttgtc ctcaacctcc caatcaaaga aaatatgaga aatctgcgag ttaagattga
    12181 gaaaaagacg ggcctactat ggaatagatg gcaaacaatc tatgaaaaca gaccaatttc
    12241 agctcaaccc caccgtaaaa ttacccattg gggtacgaca ttgaattcca aggtgagtga
    12301 cgatgatgtc ttgtaatctg atggtacaat gacagttagt ttgtctagtt tataagaaag
    12361 tactacctga gcttgaatag aactcaggta gctctctgtg aaagaacaaa att
//
LOCUS       DAVBCW010000054        11954 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVBCW010000054 DAVBCW010000000
VERSION     DAVBCW010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11954
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.054"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(101..1732)
                     /locus_tag="VQ514_001291"
     CDS             complement(101..1732)
                     /locus_tag="VQ514_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HET2500044.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGVGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGITITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
     gene            2025..7004
                     /locus_tag="VQ514_001292"
     CDS             2025..7004
                     /locus_tag="VQ514_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HET2500045.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHARENKLEKAEGVAIASETASPASNEAATTE
                     TAEAASAAKPEEKASEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAAEANKTEK
                     EVQPDVPKNTEKTLKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNE
                     KASKEAKVQALSNTNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDA
                     GHRNGVPVYGTLFFNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFI
                     NQETTGDLVKPLGEKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYN
                     YQFMQPEGDKVPADNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKT
                     KVKWNDILDENGKLRLSLGLFAPDAITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGD
                     KDWYGIANLVADRTPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWR
                     WWQTSTGEKLRAEYDFTDAYNGGNSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVA
                     HKGGKGSKVYMAFSTTPDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFF
                     EHEGAVKDYQFNLGQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADF
                     YEVYEKDGDSWKLLTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTT
                     FDWGMTVKDTSLPKPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSA
                     QLSGSVDIRLTKPRTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEV
                     RGNKAHVTDITLDKPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMA
                     KAAARSLGNNKVQVGFADVPAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQ
                     SGLLYYRTQLPGKEISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLR
                     VQYEGGTEDELIRLTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEAS
                     PKTILGIEVSQEPKKDYLVGDSLDLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQK
                     TGRQTLTLRYQGHEVNFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASSEVKEA
                     FLKAIEAAEQVLKDHETSTQDQVNDRLNKLTEAHKALNGQEKFTEEKTELDRLTGEVQ
                     ELLAAKPNHPSGSALAPLLEKNKALVEKVDLSPEELTTAKQSLKDLIALLKEDKPAVF
                     SDSKTGVEVHFSNKEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVD
                     LSYASIVKIPIEKDKKVKKVFFLPEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESA
                     EKPQPAKPAPQNTVLPKPTYQPTSDQQKAPKLEVQEEKVAFHRQEHENTEMLVGEQRV
                     IIQGRDGLLRHVFEVDENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQ
                     NTAVKSEEASKQLPNTGTADANEALIAGLASLGLASLALTLRRKREDKD"
     gene            7094..8290
                     /locus_tag="VQ514_001293"
     CDS             7094..8290
                     /locus_tag="VQ514_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET2500046.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            8426..8953
                     /locus_tag="VQ514_001294"
     CDS             8426..8953
                     /locus_tag="VQ514_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HET2500047.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETISYVFVRVSETFMGVFVAIIVN
                     YDIDRVRLFLEKKEK"
     gene            9030..9386
                     /gene="glnR"
                     /locus_tag="VQ514_001295"
     CDS             9030..9386
                     /gene="glnR"
                     /locus_tag="VQ514_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HET2500048.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRCALHNEL
                     LQQGRFASVQSPFGRG"
     gene            9423..10769
                     /gene="glnA"
                     /locus_tag="VQ514_001296"
     CDS             9423..10769
                     /gene="glnA"
                     /locus_tag="VQ514_001296"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HET2500049.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYIAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(10865..10984)
                     /locus_tag="VQ514_001297"
     CDS             complement(10865..10984)
                     /locus_tag="VQ514_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HET2500050.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            11242..11379
                     /locus_tag="VQ514_001298"
     CDS             11242..11379
                     /locus_tag="VQ514_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500051.1"
                     /translation="MNPDRAEEYFCRGCQGENPEDIEFYDEQLQAEKVEALNIRLEVK
                     N"
     gene            11550..11954
                     /locus_tag="VQ514_001299"
                     /pseudo
     CDS             11550..11954
                     /locus_tag="VQ514_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220748.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
BASE COUNT         3841 a         2183 c         2566 g         3364 t
ORIGIN      
        1 tactatatat ctgcagaata ctctttcagt ttcattcagc ctagtcttgc ttaggaaaaa
       61 gtaaaaagcc atccaattat gatggatagc ttcttggttc ttagatttgt tctgcaaaaa
      121 ccttttcagc aaggttcata gcgtggtctg atacacgagt gtagtgtgag atgatgtcga
      181 taaagttgac cccagcttgt gttgaacatt cgcctttgtt gaggcgtttg atgtgggttt
      241 ttctgagaac acgttctatc ttattgattg cttcatgacg ttctacaaga ctgcgtgctt
      301 tttcaatatc attgttttcc acactatcca gagcatcttt gataaagtca ctagtttggc
      361 ggtaaacttc ctctaattct ttcaaggcgg catcagaaaa ttcaacattt ttccgttgaa
      421 gatagtcagt cagattgagt agagcctccg tgtggtctcc aatccgttcc aaatcacggg
      481 aggaatcaag gatattggta agcacttcac tttctttttg gctgagagct tcgcttgaaa
      541 gggcaatgag ataacgtgtt aattgctcat cgatggtgtt aattgcttct tcggttttat
      601 gccctttttc agcaactttt tcatccaagt caatgatgta cttataggaa aggtcaaagg
      661 ctttagcagc gtagtttcct aagtgcaaga gctctttctt agcatttcct agagcgatag
      721 atggggcctg tttgatgaaa tgttcatcaa gatataaggg ttcgtatttg acaacctcgt
      781 cctctccagg aataatcttg gttacaaagt aagccagagc tccgataaat ggaaattgga
      841 caatggtgtt ggtaatatta aaggttccgt gagcaaaggc gatggtcatt tccggtgcta
      901 gatttagcgt agcttcaaac caatgaatca ggacagtaaa aggaactaga aaaataacgc
      961 agacgactgt tccgataacg ttgaaggcaa catgagctcc tgctacccgt ttagctgcaa
     1021 tattagcccc taaagaggca atgatggctg taatggttat cccgatattg tcaccaaata
     1081 gaactggcaa agctccctgt aggtcaatta gattgccggc gtagaggttt tgtaaaatcc
     1141 caatggtagc cgaagaagct tgaattagca aggtcaagcc ggtaccgaca aagacaccca
     1201 aaacaggatt cttacttagc tcaatcatat agtccttaaa gacctgtaaa tccttgagtg
     1261 gagccattgc gccgctcatg agattgaggg caaaaaagat accaccgaca ccaaagagga
     1321 tgcgtccgat attattgact gtccgatttt ttgtaaaaaa gagacagacg gcaccgataa
     1381 agagcatagg tagggcatag ttacctaatt taaaaccgat gagaaaggat gtgacagttg
     1441 tcccaatatt agcacccatg acaatcccga tagcctgacg taaggttaag agaccggcac
     1501 tgaccaggcc gactgtgata actgttacac cagaactaga ctgaattaga gcagtcatcc
     1561 caataccaac cagaactcca aagaaaggat tactagtata tttgtcaatg taaaaacgaa
     1621 ggcgatctcc agcagcttgt tgcaaaccgt ctcccatggt cttgatgcta tataagaata
     1681 gccccagacc acctaaaaag tgaaataaaa tttcctgcca attaatggac atttcttttt
     1741 cctccgaaaa ataatcgcgg aatatcttct attctatttt aaaggataaa agtaaatcta
     1801 acaagtgtta atgtcaagtt tgtaaaagaa aattataatt gataagtgta ttatttttaa
     1861 gagtttttaa ccttatttta gatttgtgca aacagttatt gttttttgtc taaaaatgtt
     1921 ttaggataaa aatgtgatta tcatggttct agagtttgat aaaaaactac agaatataaa
     1981 gcgctttctt gaaaacaaca aaatcaatct tttaggagga gaaaatgaag aatccatttt
     2041 ttgaaagacg ttgtcgttac agtattcgta agttatcagt aggagcctgc tcgctgatga
     2101 ttggtgctgt tttatttgct ggtccagcct tggctgaaga aactgcagtt cctgaaaata
     2161 gcggagctaa tacagagctt gtttcaggag agagtgagca ttcgaccaat gaagctgata
     2221 agcagaatga aggggaacat gctagagaaa acaagctaga aaaggcagaa ggagtagcga
     2281 tagcatctga aactgcttcg ccagcaagca atgaagctgc aactactgaa actgcagaag
     2341 cagctagcgc agctaaacca gaggaaaaag caagtgaggt ggttgcagaa acaccatctg
     2401 cagaagcaaa acctaagtct gacaaggaaa cagaagcaaa gcccgaagca actaaccaag
     2461 gggatgagtc taaaccagca gcagaagcta ataagactga aaaagaagtc cagccagatg
     2521 tccctaaaaa tacagaaaaa acattaaaac caaaggaaat caaatttaat tcatgggaag
     2581 aattgttaaa atgggaacca ggtgctcgtg aagatgatgc tattaaccgc ggatctgttg
     2641 tcctcgcttc acgtcggaca ggtcatttag tcaatgaaaa agctagcaag gaagcaaaag
     2701 ttcaagcctt atcaaacacc aattctaaag caaaagacca tgcttctgtt ggtggagaag
     2761 agttcaaggc ctatgctttt gactattggc aatatctaga ttcaatggtc ttctgggaag
     2821 gtctcgtacc aactcctgac gttattgatg caggtcaccg taacggggtt cctgtatacg
     2881 gtacactctt cttcaactgg tctaatagta ttgcagatca agaaagattt gctgaagctt
     2941 tgaagcaaga cgcagatggt agcttcccaa ttgcccgtaa attggtagac atggccaagt
     3001 attatggcta tgatggctat ttcatcaacc aagaaacaac tggagatttg gttaaacctc
     3061 ttggagaaaa gatgcgccag tttatgctct atagcaagga atatgctgct aaggtaaacc
     3121 atccaatcaa gtattcttgg tacgatgcca tgacctataa ctatggacgt taccatcaag
     3181 atggtttggg agaatacaac taccaattca tgcaaccaga aggagataag gttccggcag
     3241 ataacttctt tgctaacttt aactgggata aggctaaaaa tgattacact attgcaactg
     3301 ccaactggat tggtcgtaat ccttatgatg tatttgcagg tttggaattg caacagggtg
     3361 gttcctacaa gacaaaggtt aagtggaatg acattttaga cgaaaatggg aaattgcgcc
     3421 tttctcttgg tttatttgcc ccagatgcca ttacaagttt aggaaaaact ggtgaagatt
     3481 atcataaaaa tgaagatatc ttctttacag gttatcaagg agaccctact ggccaaaaac
     3541 caggtgacaa agattggtat ggtattgcta acctagttgc ggaccgtacg ccagcggtag
     3601 gtaatacttt tactacttct tttaatacag gtcatggtaa aaaatggttc gtagatggta
     3661 aggtttctaa ggattctgag tggaattatc gttcagtatc aggtgttctt ccaacatggc
     3721 gctggtggca gacttcaaca ggggaaaaac ttcgtgcaga atatgatttt acagatgcct
     3781 ataatggcgg aaattccctt aaattctctg gtgatgtagc cggtaagaca gatcaggatg
     3841 tgagacttta ttctactaag ttagaagtaa ctgagaagac caaacttcgt gttgcccaca
     3901 agggaggaaa aggttctaaa gtttatatgg cattctctac aactccagac tacaaattcg
     3961 atgatgcaga tgcatggaaa gagctaaccc tttctgacaa ctggacaaat gaagaatttg
     4021 atctcagctc actagcaggt aaaaccatct atgcagtcaa actatttttc gagcatgaag
     4081 gtgctgtaaa agattatcag ttcaacctcg gacaattaac tatctcggac aatcaccaag
     4141 agccacaatc gccgacaagc ttttctgtag tgaaacaatc tcttaaaaat gcccaagaag
     4201 cggaagcagt tgtgcaattt aaaggcaaca aggatgcaga tttctatgaa gtttatgaaa
     4261 aagatggaga cagctggaaa ttactaactg gctcatcttc tacaactatt tatctaccaa
     4321 aagttagccg ctcagcaagt gctcagggta caactcaaga actgaaggtt gtagcagtcg
     4381 gtaaaaatgg agttcgttca gaagctgcaa ccacaacctt tgattggggt atgactgtaa
     4441 aagataccag cctaccaaaa ccactagctg aaaatatcgt tccaggtgca acagttattg
     4501 atagtacttt ccctaagact gaaggtggag aaggtattga aggtatgttg aacggtacca
     4561 ttactagctt gtcagataaa tggtcttcag ctcagttgag tggtagtgtg gatattcgtt
     4621 tgaccaagcc acgtaccgtt gttagatggg tcatggatca tgcaggagct ggtggtgagt
     4681 ctgttaacga tggcttgatg aacactaaag actttgacct ttattataaa gatgcagatg
     4741 gtgagtggaa gctagctaag gaagtccgtg gtaacaaagc acacgtgaca gatatcactc
     4801 ttgataaacc aatcactgct caagactggc gcttgaatgt tgtcacttct gacaatggaa
     4861 ctccatggaa ggctattcgt atctataact ggaaaatgta tgaaaagctt gatactgaga
     4921 gtgtcaatat tccgatggcc aaggctgcag cccgttctct aggcaataac aaggtacaag
     4981 ttggctttgc agatgtaccg gctggagcaa ctattaccgt ttatgataat ccaaattctc
     5041 aaactccgct cgcaaccttg aagagcgaag ttggaggaga cctagcaagt gcaccattgg
     5101 atttgacaaa tcaatctggt cttctttatt atcgtaccca gttgccaggc aaggaaatta
     5161 gtaatgtcct agcagtttcc gttccaaaag atgacagaag aatcaagtca gtcagcctag
     5221 aaacaggacc taagaaaaca agctacgccg aaggggagga tttggacctt agaggtggtg
     5281 ttcttcgagt tcagtatgaa ggaggaactg aggacgaact cattcgccta actcacgcag
     5341 gtgtatcagt atcaggtttt gatacgcatc ataagggaga acagaatctt actctccaat
     5401 atttgggaca accagtaaat gctaatttgt cagtgactgt cactggccaa gatgaagcaa
     5461 gtccgaaaac tattttggga attgaagtaa gtcaggaacc gaaaaaagat tacctagttg
     5521 gtgatagctt agacttgtct gaaggacgct ttgcagtggc ttatagcaat gacaccatgg
     5581 aagaacattc ctttactgat gagggagttg aaatttctgg ttacgatgct caaaagactg
     5641 gtcgtcaaac cttgacgctt cgttaccaag gtcatgaagt caactttgat gttttggtat
     5701 ctccaaaagc agcattgaac gatgagtacc tcaaacaaaa attagcagaa gttgaagctg
     5761 ctaagaacaa ggtggtctat aactttgctt catcagaagt aaaagaagcc ttcttgaaag
     5821 caattgaagc ggccgaacaa gtgttgaaag accatgaaac tagcacccaa gatcaagtca
     5881 atgaccgact taataaattg acagaagctc ataaagctct gaatggtcaa gagaaattta
     5941 cggaagaaaa gacagagctt gatcgcttaa caggtgaggt tcaagaactc ttggctgcca
     6001 aaccaaacca tccttcaggt tctgccctag ctccgcttct tgagaaaaac aaggccttgg
     6061 ttgaaaaagt agatttgagt ccagaagagc ttacaacagc gaaacagagt ctaaaagatc
     6121 tgattgcttt attgaaagaa gacaagccag cagtcttttc tgatagtaaa acaggtgttg
     6181 aagtacactt ctcaaataaa gagaagactg tcatcaaggg tttgaaagta gagcgtgttc
     6241 aagcaagtgc tgaagagaag aaatactttg ctggagaaga tgctcatgtc tttgaaatag
     6301 aaggtttgga tgaaaaaggt caagatgttg atctctctta tgcttctatt gtgaaaatcc
     6361 caattgaaaa agataagaaa gttaagaaag tatttttctt acctgaaggc aaagaggcag
     6421 tagaattggc ttttgaacaa acggatagtc atgttatctt tacagcacct cactttactc
     6481 attatgcctt tgtttatgaa tctgctgaaa aaccacaacc tgctaaacca gcaccacaaa
     6541 acacagtcct tccaaaacct acttatcaac cgacttctga tcaacaaaag gctcctaaat
     6601 tggaagttca agaggaaaag gttgcctttc atcgtcaaga gcatgaaaat actgagatgc
     6661 tagttgggga acaacgagtc atcatacagg gacgagatgg actgttaaga catgtctttg
     6721 aagttgatga aaacggtcag cgtcgtcttc gttcaacaga agtcatccaa gaagcgattc
     6781 cagaaattgt tgaaattgga acaaaagtaa aaacagtacc agcagtagta gctacacagg
     6841 aaaaaccagc tcaaaataca gcagttaaat cagaagaagc aagcaaacaa ttgccaaata
     6901 caggaacagc tgatgctaat gaagccctaa tagcaggctt agccagcctt ggtcttgcta
     6961 gtttagcctt gaccttgaga cggaaaagag aagataaaga ttaaatatcg aaaaatcttg
     7021 tgaaatcttt ccttatattt ccaaagtgtg atataatagt ttcgaataaa ataaataaag
     7081 gagtttttgt aacatggcaa aacttactgt taaagacgtt gacttgaaag gtaaaaaagt
     7141 cctcgttcgt gttgacttca acgtaccatt gaaagatggc gtaatcacta acgataaccg
     7201 tatcacagca gctcttccaa ctattaagta catcatcgaa caaggtggac gtgcaattct
     7261 tttctctcac cttggacgtg tgaaagaaga agctgataaa gctggtaaat cacttgctcc
     7321 tgtagcagct gacttggcag caaaacttgg tcaagatgtt gttttcccag gtgtcactcg
     7381 tggtgctgaa ttggaagcgg caatcaacgc tcttgaagat ggacaagttc tcttggttga
     7441 aaacactcgt tacgaagatg ttgacggcaa gaaagaatct aaaaacgatc ctgaacttgg
     7501 taaatactgg gcatcacttg gagatggtat cttcgtaaac gatgcattcg gtacagctca
     7561 ccgtgcacac gcatctaacg ttggtatctc agcaaacgtt gaaaaagcag ttgctggttt
     7621 ccttcttgaa aacgaaattg cctacatcca agaagcagtt gaaactccag aacgtccatt
     7681 cgtggctatc cttggtggtt caaaagtttc agacaagatc ggtgttatcg aaaacttgct
     7741 tgaaaaagct gataaagtcc ttatcggtgg tgggatgact tacacattct acaaagcaca
     7801 aggtatcgaa atcggtaact cacttgtaga agaagacaaa ttggatgttg cgaaagctct
     7861 tcttgaaaaa gcaaatggta aattgatctt gccagttgac tcaaaagaag ctaacgcatt
     7921 tgctggttac actgaagtgc gtgacactga aggtgaagca gtttctgaag gcttccttgg
     7981 tcttgacatc ggtccaaaat ctatcgccaa atttgacgaa gctttgactg gtgccaaaac
     8041 agttgtatgg aacggaccta tgggtgtatt tgaaaaccca gatttccaag ctggtacaat
     8101 cggtgtgatg gacgctatcg tgaaacaacc aggagttaaa tcaatcatcg gtggtggtga
     8161 ctcagctgcc gcagcgatta accttggccg tgcagacaag ttctcatgga ttagtacggg
     8221 tggtggagca tcaatggaac ttcttgaagg taaggttctt ccaggacttg cagccttgac
     8281 agaaaaataa gattttataa acaaatcaaa gaagagaggg atgaaagttc ctcttttctt
     8341 ttgcttaaaa taaaaacgct tcctctcaac tattactcat aaaaatcacc gatttatgat
     8401 aaaatggaaa tagaaagttg agattatgag ttattttaaa aaatataaat tcgataaatc
     8461 ccagttcaaa cttggtatgc gaacctttaa aacaggtatt gctgtttttc tagttctctt
     8521 gatttttggc ttttttggct ggaaaggtct tcaaattggt gctttgacag ccgtttttag
     8581 cctgagggag agttttgatg agagtgttca ttttgggact tcgcgtattc taggaaatag
     8641 tatcggtgga ctctatgcct tggtcttctt cttattaaat acctttttcc acgaagcctt
     8701 ttgggtgacc ttggtagttg ttccaatctg caccatgtta accattatga caaatgtagc
     8761 catgaataac aaagcagggg ttattggtgg tgtagcagct atgttaatca ttaccctatc
     8821 aattccaagt ggtgagacaa tttcgtacgt gtttgtgcgt gtatcagaaa cgtttatggg
     8881 agtttttgtc gcaattatcg taaattacga tattgatcgt gttcgtctct ttttagagaa
     8941 aaaagaaaaa taatgttaca ttttataaca ttatcaattg acgtttgtct ttttttagac
     9001 tataatagac agaaagaagg aaattgtaaa tgaaggaaaa agaatttcgc cgaaatatgg
     9061 ctgtttttcc tatcggcagt gttatgaagt tgaccgatct atcggcgcgt cagattcgtt
     9121 attatgaaga tcaagagttg atcaagcccg atcgaaacga aggaaatcgt cgcatgtatt
     9181 ccttgaatga catggatcgt ctgcttgaaa tcaaagatta tatctctgaa ggttataata
     9241 tcgctgccat taagaaaaaa tatgctgaac gtgaagcgaa atccaagaaa gcggttagtc
     9301 agactgaagt acgttgtgca cttcacaatg aactcctcca acaggggcgt tttgcttcag
     9361 tacagtcacc ttttggtcgc ggttaggcaa ccgcaagtag tcatagatta aggagaaaac
     9421 ttatgccaat cacagctgca gatattcgtc gtgaagtcaa ggaaaaaaat gttaccttta
     9481 ttcgtcttat gttctcagat attttgggaa ccatgaaaaa cgtcgaaatt cctgctacag
     9541 atgaacagtt agataaggtc ttgtcgaaca aggttatgtt tgatggatct tctattgaag
     9601 gttttgtacg tatcaatgag tcggatatgt acttgtaccc ggacttggat acatggacag
     9661 tcttcccttg gggagatgaa aatggaagtg ttgcaggtct gatctgtgat gtctatacaa
     9721 cagaaggtga accatttgcg ggtgaccctc gtggtaattt gaaacgagct cttcgtcaca
     9781 tggaagaagt tggattcaaa tccttcaacc ttggtccaga gccagaattc ttcctattta
     9841 agttggatga aaatggggac ccaacacttg aagtgaatga caagggtggc tactttgatt
     9901 tggcacctac tgaccttgcg gacaacacac gtcgtgagat tgtgaatgtc ttgaccaaaa
     9961 tgggatttga agtagaagcg agtcaccacg aggttgcggt tggacagcat gagattgact
    10021 ttaagtacga tgaagttctc cgtgcttgtg ataagattca aatctttaag cttgttgtta
    10081 aaaccattgc tcgcaaacac ggactttacg caacatttat ggcgaagcca aaatttggta
    10141 ttgctggatc aggtatgcac tgtaatatgt ccttgtttga tgcagaagga aataacgcct
    10201 tctttgatcc aaatgatcca aaaggaatgc agttgtcaga aacagcttac catttcctag
    10261 gcggtttgat caagcatgct tacaactata ctgccatcat gaacccaaca gttaactcat
    10321 acaaacgttt ggttccaggt tatgaagcgc ctgtttacat tgcttgggct ggtcgtaacc
    10381 gttcgccact tgtgcgcgta cctgcttcac gtggtatggg aactcgtctt gagttgcgtt
    10441 cagtggatcc aatggcgaac ccttacattg ctatggctgt tcttttggaa gttggtttgt
    10501 atggtattga aaataaaatc gaagcaccag ctcctatcga agaaaatatc tacatcatga
    10561 cagcagaaga gcgcaaggaa gctggtatta cagaccttcc atcaactctt cacaacgctt
    10621 tgaaagcttt gacagaagat gaagtggtta aagctgctct cggagatcac atctacacta
    10681 gcttccttga agccaaacga atcgaatggg caagttatgc aaccttcgtt tcacaatggg
    10741 aaattgataa ttatttagac ctttactaat actaatatag aagaaagatt gcctgtgggt
    10801 aaacagtcac acagcagtct aagccatcaa atacctataa cccttaaaat agcgatttcg
    10861 gtcgctattt atttttcgaa aaatcctctt gactcttttg cgaattagat ttatcatcag
    10921 acgtgtagaa gaaaatcaag gagtcactat ggaaaaattc aatttcaaaa acaatatcgg
    10981 gcaagaaaac aagttacttc aaatcgaaat ctacaagttt actaactttt gcaagcttca
    11041 aaattacaca tctgtaaata ttttttcgaa agatatattt gaagccatag taaattgaga
    11101 ctagaatagt acactacatc tgctaaaaat ttctagaaat taatttaact ttcctaatcg
    11161 atttgtttac atcttatttc aatctactat attatctcaa taaatgatga tggcgactgt
    11221 tttgtttgtg cttgtcgatt tttgaatcct gatcgagcgg aagaatattt ctgccgaggt
    11281 tgtcaagggg aaaatcctga agatattgaa ttttatgatg aacaattaca ggcagaaaag
    11341 gtggaggctt tgaatattcg attggaagta aaaaattgaa ttatctacaa tctcatcaac
    11401 aataaaccta tttcaacccc attatctata ggcgtatttt tacgcctttt tatatacttg
    11461 aagcacataa gaaacattat gggaagtttg ctgatggaag tactcaagaa attgatgttc
    11521 cttatgatat tcctgatact tgggagtggg tgaggataaa ataaatttat tggaattttg
    11581 ggcaaaataa gccagagaaa tcctttaggt atatagatac gtctagtatt gatagaaaaa
    11641 agaacataat caactacaaa aatctacaat atctttcacc tgaacaagcg ccttcccgtg
    11701 ccagaaaatt agtttcgcag aatagtgtct tattttcaac agttagacca tatctaaaaa
    11761 atattgctgt agttagagaa cttaaagagt atttgatagc tagtacagca tttattgttt
    11821 tggatacttt acttaacgaa acatatttga aatattattt attatcagat aattttatca
    11881 atcgtgttaa taataaatct actggaacaa gttatcctgc aatcaatgat tataatttta
    11941 atctattatt aatt
//
LOCUS       DAVBCW010000055        11793 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVBCW010000055 DAVBCW010000000
VERSION     DAVBCW010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11793
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.055"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            219..2510
                     /gene="pcrA"
                     /locus_tag="VQ514_001300"
     CDS             219..2510
                     /gene="pcrA"
                     /locus_tag="VQ514_001300"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HET2500052.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTTYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKSASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            2553..3233
                     /gene="radC"
                     /locus_tag="VQ514_001301"
     CDS             2553..3233
                     /gene="radC"
                     /locus_tag="VQ514_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HET2500053.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(3230..3919)
                     /locus_tag="VQ514_001302"
     CDS             complement(3230..3919)
                     /locus_tag="VQ514_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET2500054.1"
                     /translation="MKKPVIGITGNEKAHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYNLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(3937..4578)
                     /locus_tag="VQ514_001303"
     CDS             complement(3937..4578)
                     /locus_tag="VQ514_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HET2500055.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            complement(4661..4876)
                     /locus_tag="VQ514_001304"
     CDS             complement(4661..4876)
                     /locus_tag="VQ514_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HET2500056.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGQKVYQGLVGDLYRFLSHADFLHQN"
     gene            complement(4884..5231)
                     /locus_tag="VQ514_001305"
     CDS             complement(4884..5231)
                     /locus_tag="VQ514_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HET2500057.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFSLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(5236..6351)
                     /locus_tag="VQ514_001306"
     CDS             complement(5236..6351)
                     /locus_tag="VQ514_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET2500058.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLEAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(6361..7320)
                     /locus_tag="VQ514_001307"
     CDS             complement(6361..7320)
                     /locus_tag="VQ514_001307"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HET2500059.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            complement(7433..8002)
                     /locus_tag="VQ514_001308"
     CDS             complement(7433..8002)
                     /locus_tag="VQ514_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HET2500060.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAEDYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDITDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            8130..8801
                     /locus_tag="VQ514_001309"
     CDS             8130..8801
                     /locus_tag="VQ514_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HET2500061.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            8785..9603
                     /locus_tag="VQ514_001310"
     CDS             8785..9603
                     /locus_tag="VQ514_001310"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HET2500062.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            9600..10496
                     /locus_tag="VQ514_001311"
     CDS             9600..10496
                     /locus_tag="VQ514_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET2500063.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIDIPAEEGFETLEAIEHPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            10540..11514
                     /gene="pta"
                     /locus_tag="VQ514_001312"
     CDS             10540..11514
                     /gene="pta"
                     /locus_tag="VQ514_001312"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HET2500064.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
BASE COUNT         3431 a         2259 c         2538 g         3565 t
ORIGIN      
        1 attatcaatc atgaactagg attctagttt tgcaggaagt ctattgaggt attgagctag
       61 tttatgaaaa aattgggtga aaaagtcgag tgttttagaa acccacagtg tagtattcta
      121 gtttcaatcc actatatttt gctactcccc gtaaagtttc tattttccct gatttctgat
      181 ataatagaaa tattgacttc aagagtaagg aagagaagat gaacgcatta ttaaatggaa
      241 tgaatgaccg tcaggctgag gcggtgcaaa cgacagaagg tcccttgcta atcatggcag
      301 gagctggttc tggaaagact cgtgttttaa cccaccgtat cgcttatttg attgatgaaa
      361 agctggtcaa tccttggaat atcttggcca ttacctttac caacaaggct gcgcgtgaga
      421 tgaaagagcg tgcttatagc ctcaatccag ctactcagga ctgtctgatt gcgaccttcc
      481 actccatgtg tgtgcgtatt ttgcgtcgcg atgcggacca tattggctac aatcgtaatt
      541 ttacaattgt ggatcctggt gaacagcgaa cgctcatgaa acgtattctc aaacagttga
      601 acttggatcc taaaaaatgg aatgaacgaa ctattttggg gaccatttcc aatgctaaga
      661 atgatttgat tgatgatgtt gcttatgctg ctcaagctgg cgatatgtat acgcaaattg
      721 tggcccagtg ttatacaacc tatcaaaaag aacttcgtca gtctgaatcc gttgactttg
      781 atgatttgat tatgctgacc ttgcgtctct ttgatcaaaa tcctgatgtt ttgacctact
      841 accagcaaaa attccaatac atccacgttg atgagtacca agataccaac cacgctcagt
      901 accaattggt caaactcttg gcttcccgtt ttaaaaatat ctgtgtggtt ggggatgcgg
      961 accagtctat ctacggttgg cgtggtgctg atatgcagaa tatcttggac tttgaaaagg
     1021 attaccccaa agctaaggtt gttttgttgg aggaaaatta ccgctcaacc aaaaccattc
     1081 tccaagcggc caacgaggtt attaaaaata ataaaaatcg ccgtcctaaa aatctctgga
     1141 ctcaaaacgc tgatggggag caaatcgttt actatcgtgc tgatgatgag ctggatgagg
     1201 ctgtatttgt agccagaacc atcgatgaac ttagtcgcag tcaaaacttc cttcataagg
     1261 attttgcagt tctctatcgg actaatgccc agtcccgtac aattgaggaa gccctgctca
     1321 agtctaacat tccttatacc atggttggcg gaaccaaatt ctacagccgt aaggaaattc
     1381 gcgatattat tgcttatctc aaccttattg ctaatttgag tgacaacatt agttttgagc
     1441 gtattatcaa cgagcctaaa cgtggaattg gtctaggtac agttgagaaa atccgtgatt
     1501 ttgcaaattt gcaaaatatg tctatgctgg atgcttctgc taatattatg ttgtctggta
     1561 tcaagggtaa ggcagcccaa tctatctggg attttgccaa tatgatgctt gatttgcggg
     1621 agcagctaga ccacttaagc attacagagt tggttgagtc cgtcctagaa aaaacaggtt
     1681 atgtcgatat tcttaacgcc caagcgactc tagaaagcaa ggcaagggtt gaaaatatcg
     1741 aagagtttct ttctgttacg aagaactttg atgacaccac tgatgtgaca gaagaggaaa
     1801 ctggtctgga caaactgagt cgtttcttaa atgacttggc tttgattgcc gacacagatt
     1861 caggtagtca ggagacatca gaagtgacct tgatgaccct gcatgctgcc aaaggtctcg
     1921 aatttccagt tgtctttttg attgggatgg aagaaaatgt ctttccactt agtcgtgcga
     1981 ctgaagatcc agatgaatta gaagaagagc gccgtctagc ctatgtaggt atcacgcgtg
     2041 cagagaaaat tctctatctg accaatgcca actcacgctt gctttttggt cgtaccaatt
     2101 ataaccgtcc gactcgtttt attaacgaaa tcagttcaga cttgcttgag tatcaaggtc
     2161 tggctcgtcc tgcaaataca agctttaagg catcatatag cagtggtagt atttcctttg
     2221 gtcaaggtat gagtttggct caggctcttc aagaccgtaa acgcggtgct gccccaaaat
     2281 cgatccagtc aagcggtctt ccatttggtc aatttacagc tggcgcaaaa tcagcatcta
     2341 gcgaggcaaa ttggtccatt ggtgacattg ctctccacaa gaaatgggga gagggaactg
     2401 ttctggaagt ttcaggtagc ggtgctaggc aggaattgaa aatcaatttc ccagaagtag
     2461 gtttgaaaaa acttttagcc agtgtggctc caattgagaa aaaaatctaa ttttccatcc
     2521 ttctcacgaa taataaagtg aggaggattt ttatgtacag tatttcattc caagaagatt
     2581 cactattacc aagagaaagg ctggccaagg aaggagttga agcgcttagt aaccaagagt
     2641 tgctagctat tttactcagg acaggaacac gtcaagctag cgtttttgaa attgcccaaa
     2701 aagtcttgaa caatctttca agcctaacgg atttgaaaaa aatgaccctg caggaattgc
     2761 agagtttgtc tggtattggg cgtgttaagg ccatagaatt acaagctatg attgaactgg
     2821 ggcatcgtat tcacaaacac gagactcttg aaatggaaag tattctcagc agtcaaaagt
     2881 tggccaagaa gatgcagcag gaattagggg ataaaaaaca agagcacctg gtggcactct
     2941 atctcaatac tcaaaatcaa atcatccatc agcagaccat ttttatcggt tctgtaactc
     3001 gtagtatcgc tgaaccgcga gagattcttc actatgcaat caagcatatg gcgacttctc
     3061 ttatcttggt ccacaatcat ccttcaggag cggtagcgcc tagccaaaat gatgatcatg
     3121 tcactaaact tgttaaagaa gcctgcgaat tgatggggat tgttctcttg gaccatttga
     3181 ttgtctctca ttctaattac tttagttatc gtgaaaagac agatttaatc taaagttcat
     3241 taacgacata gtcaaagagt tttttatctt tgggacgatt ttcaaaaaga agttctggat
     3301 gccattggac accgagaaag gcgacatcat ccgtactcat gacagcctca atgataccat
     3361 ctttaggatc atgagccaca acttttaaat ttggtgctaa gtccttgatg ctctggtggt
     3421 ggaaggagtt gatatgagag atttctccat agatttcttg gagaacggta tctggttctg
     3481 ttaccaagcg ttgagttgtg tactcaacag aagaatcctg ccaatggtct tcgatatctt
     3541 ggtacaaagt tccacccatg gcaacgttaa agagttgggt accacggcag acagagaaaa
     3601 tgggcttttt ctgtttaata gcttccttga tgagggccag ttcgaagata tctctttgaa
     3661 ggttatagtc atcgctatca atggttttgg gttcgccata aaattttgga tcgacatttt
     3721 gcccacctgt caagatgagc ttgtcaatca aactgatata gtggcaggcc atttcttgat
     3781 caccaatcgg taggatgatg ggaatccctc cagcatcttt aacgccttca acaaagcctt
     3841 ttgctgcgta gctcatcatg atgtcatcat ctggatgagc tttttcgttt cctgtaatcc
     3901 caataactgg ttttttcata aaatgatttt cgctttctaa tcctcttttc gcatgaagta
     3961 gaggagggtt tggagttcac ttgtcaaatc gacatactga acgaccacgt cttttggtaa
     4021 atgcagatgg actggtgaaa aactgagaat tcctttcaca ccagcatcaa ccaagagatt
     4081 agcaacctct tgtgacttga cgctgggaac agttaggata gcagtcttca catcagcatc
     4141 cttgatttta tccttgatct gagaaatccc gtaaatggga atcccgtcag gagtttgggt
     4201 accgacttca ggatggtcgt ctaggtcaaa ggccatgata atcttcatct tgttacgttc
     4261 gtggaagcgg tagtggagaa gggcatggcc catatttcca ataccaacca gcatgacatt
     4321 ggtaatagag ttgtcattga gcaaatcggc aaaaaatgtc attagttttt tgacatcata
     4381 gccaaaacca cgacgaccaa gttcaccaaa ataggaaaaa tcacgacgta cggtcgctga
     4441 atcaataccg atagcctctg caatttgctt agagttggca cgttcaatct tttctgcatg
     4501 aaatctctta aaaattcgat agtagagaga gagtcttttt gctgtagctt ttggaatagc
     4561 aaactgttta tctttcacaa aatcacaacc tttctattct tctattttat agaaacattg
     4621 tgaaaaaatc aacaaaaata agaaaaaact aagaaaaatc ttagttttga tgtaaaaaat
     4681 ctgcatgaga tagaaaacgg tagaggtctc cgaccagacc ctgataaact ttttggcccc
     4741 taaaagtcag agaagtcaca taaagtgtat ctggtaaggt tacacatcct gacaaagtca
     4801 acatgagagc ctcatgatcc tcatacttga gagtacgctc tacatgatag cagtccttat
     4861 aggtcagttc aaacattttg gctctatctt tccgattttg taaagacacc acgttctacc
     4921 aagctatcca tgaggaagta gaatttttcc tgatgaatat ggtggtcttc tgatttgaaa
     4981 atatcaacta gacgaaggct aaacttgtca gtgatattga ttttagcccc tgtaagttcc
     5041 ttgttaatga tgattttgag ttggaagcct tcaccgctgt ttggcacttt ttccaaaagg
     5101 cgagtcagtt catagttacc aaccttagtt tcaaaaaagg tgttatcttt gagggtgaat
     5161 tttttaacag aagggctaag agtgtaatcg taacgacaat tttttaactg aatgattttt
     5221 tcaaatgcca tatggctaac ctccgataat ttcttttaag gtttttgcga gggtttgtag
     5281 gtcttcaacg gtattttgtg gcgacaaact gatgcgaagg gattccttca agcgttctga
     5341 atttgcgcca tacatggctt caagaacatg gctggattgg acaacgcctg cagtacaggc
     5401 tgagccagta gagattgaaa ttccagctaa atctagccga aggagtaaga ggtcattttt
     5461 ctgaccagga aatccaatat tgagaacata agggagatga tgttttcctc tattcaagta
     5521 atactgaatg ccctccagct ctgccagaaa ggcggtttct agattttgta catgttgaaa
     5581 atgttcttct tgtttttcta ggtcttcttt tagggctgca accatgccta cgatagcagg
     5641 cagattttca gttcctgcac gttttttctg ttcctggtct ccgccatgta gataggaatc
     5701 aaagtccatg ctagatgcgt agagaaaacc gattccctta ggaccatgga atttgtgggc
     5761 agaagcagtg agaaaatcaa tgcccaattc ttctgaatga attgggattt taccaatagc
     5821 ctgaactgca tcaacatgat aggcagcagg gtgttgcttg agtatttggc caatttcagc
     5881 gatgggcagt aggtttcctg tctcattatt ggcaaacatg gtagaaacca aaatcgtatc
     5941 gtcacgtaaa gccttttgaa tttgctgggc tgtgatttct tgattttctg gctggataat
     6001 ggttgcttca aacccaaagt gttgaaccaa gtaatcaatt gtttcaagga cagcatggtg
     6061 ctcgatggca gttgtgatga tatgttttcc ttgttcttgg tgacgaagac agtagccaat
     6121 gatggtagta ttattgcctt cagtcccacc agaagtgaaa aagatatgtt gaggttttgt
     6181 ccttagtaac tgggctagtt cctgacgggc ttctcgcaag agtttgccag cttgacgacc
     6241 atgaccatga atactagaag gatttccgtg ggtttcttgc ataaccttgg tcatagctga
     6301 aatagcaact gctgacatag gagtcgttgc agcattgtcc aaataaatca aagaatcacc
     6361 ttatttcttt ttattgtagg caaagagtgg gctgactggt tttctttcgt gaatacggac
     6421 gatagcatca ccaattaact cactagcagt gatgtagcat acatttttag gagttttttc
     6481 ttttgttgct actgaatcag tcacaagaat ttctttaata ttagtattgt caagaagctc
     6541 agcagctccc tcgacgaaga gaccgtggct agaaacagca taaatttctg tagctccttc
     6601 acgttcaacg attttagaag cttcagagaa ggtacgtcct gtatttaaaa tatcatcaat
     6661 caagatagct ttcttacctt caacatcacc aataatataa ccttcgttac gagttgcatc
     6721 gtcttgaggg tagtcgataa tggcgatagg agcatcaaga tattcagcca ggctacgcgc
     6781 acgtttgaca cctgaatttt tagggctaac gacaacaaca tctgaaccaa gcaatccttt
     6841 atcgcagtaa tgttttgcga ataggggaac agtgaaaaga ttatccactg gaatatcaaa
     6901 gaaaccttga acctgaacgg catgcaaatc aagagtcagg atacgatcaa ctccagcctt
     6961 aaccagcata ttggcaacta gttttgctgt aagtggctca cgaggacaag caatgcggtc
     7021 ttgacgtgca tagccaaaat atggaaggac aacgttgata ctgtgggcac ttgcacgcac
     7081 acaagcatcg accatgatta acaattccat taggtggttg ttgacaggga aacttgttga
     7141 ttggatgatg taaacatcat aaccacggac actttcttcg atatttactt ggatttctcc
     7201 gtctgaaaat tgacgtgatg atagttttcc aagtgggaca ccaacagctt gggcaatttt
     7261 ttgtgcaatc tcttggttag agttgagtgc gaaaagtttc atgttttttc tatctgacat
     7321 tatagaccgt cctctgtaaa ctttataaat cctagttata tttaccttac atatatgaac
     7381 tgggatttgt gtatttttat cttttctatt ttaccaaaaa atggagatta tttcagctat
     7441 ttttcatact tttgacaaat cgaaccaatt ttgaaggagc tttttggtag gaaatctgat
     7501 ttttctctaa aaattgtcgg aaatcctgtt tgccttgctc atgattttcc acttcaagct
     7561 ccaattcgta atctgttata tcaaagtatc ggctctgatc cagtgccatg agaccaatag
     7621 ctgttttcat ttcatagcga agcgttgtta gacaaccaag aacctgccag ttcttacttt
     7681 ggataccatg tttcgccaat tcatccagta ctagtccttg aggaagttct tccttactca
     7741 gatagtcctc agcatctttt agttgcaatt tttggttgta ttccatgttt ccaacactct
     7801 gcgggacttt gagtgtcaac tcagcccagt cttcaaaggt tcgaatgcgc atagcgactt
     7861 tcttttctcg cagttcaaaa tcaggcgtgt cgatgtagta atttgtttga agaacaggag
     7921 tgacacctgt gaactggtct tttagacgat tgtattcatc ttttttcaat agtgttttca
     7981 attcaatttc taaatgtttc atttttctta ccttttttta tcgttgaaag cggatttatg
     8041 gtataataag cattgtattt attgtatatg aatctggaga aaaaatcaaa gatatttttg
     8101 acggataata tgagaacaag ggagaatata tgaccttaga atgggaagaa tttctagatc
     8161 cttacattca agctgttggt gagttaaaga ttaaacttcg tggtattcgt aagcaatatc
     8221 gtaagcaaaa taagcattct ccaattgagt ttgtgaccgg tcgagtcaag ccaattgaga
     8281 gcatcaaaga aaaaatggct cgtcgtggca ttacttatgc gaccttggaa cacgatttgc
     8341 aggatattgc tggcttacgt gtgatggttc agtttgtaga tgacgtcaag gaagtagtgg
     8401 atattttgca caagcgtcag gatatgcgaa tcatacagga gcgagattac attactcata
     8461 gaaaagcatc aggctatcgt tcctatcatg tggtagtaga atatacggtt gataccatca
     8521 atggagctaa gactattttg gcagaaattc aaattcgtac tttggccatg aatttctggg
     8581 caacgataga acattctctc aactacaagt accaagggga tttcccagat gagattaaga
     8641 agcgactgga aattacagct agaatcgccc atcagttgga tgaagaaatg ggtgaaattc
     8701 gtgatgatat ccaagaagcc caggcacttt ttgatccttt gagtagaaaa ttaaatgacg
     8761 gtgtaggaaa cagtgacgat acagatgaag aatacaggta aacgaattga tctgatagcc
     8821 aatagaaaac cgcagagtca aagggttttg tatgaattgc gagatcgttt gaagagaaat
     8881 cagtttatac tcaatgatac caatccggat attgtcattt ccattggcgg ggatggtatg
     8941 ctcttgtcgg cctttcataa gtacgaaaat cagcttgaca aggtccgctt tatcggtctt
     9001 catactggac atttgggctt ctatacagat tatcgtgatt ttgagttgga caagctagtg
     9061 actaatttgc agctagatac tggggcaagg gtttcttacc ctgttctgaa tgtgaaggtc
     9121 tttcttgaaa atggtgaagt taagattttc agagcactca acgaagccag catccgcagg
     9181 tctgatcgaa ccatggtggc agatattgta ataaatggtg ttccctttga acgttttcgt
     9241 ggagacgggc taacagtttc gacaccgact ggtagtactg cctataacaa gtctcttggc
     9301 ggtgctgttt tacaccctac cattgaagct ttgcaattaa cggaaattgc cagccttaat
     9361 aatcgtgttt atcgaacact gggctcttcc attattgtgc ctaagaagga taagattgaa
     9421 cttattccaa caagaaacga ttatcatact atttcggttg acaatagcgt ttattctttc
     9481 cgtaatattg agcgtattga gtatcaaatc gaccatcata agattcactt tgtcgcgact
     9541 cctagccata ccagtttctg gaaccgtgtt aaggatgcct ttatcggtga ggtggatgaa
     9601 tgaggtttga atttatcgca gatgaacatg tcaaggttaa gaccttttta aaaaagcacg
     9661 aggtttctaa gggattgctg gctaagatta agtttcgagg tggagctatt ctggtcaata
     9721 atcaaccgca aaatgcaacg tatctattgg acgttggaga ctacgttacc attgacattc
     9781 ccgctgagga aggctttgaa accttggagg ctattgagca tccattagat attctctatg
     9841 aggatgacca ctttctagtc ttgaataaac cctatggagt ggcttctatt cctagtgtca
     9901 atcactctaa caccattgcc aattttatca agggttacta tgtcaagcaa aattatgaaa
     9961 atcagcaggt tcacattgtt accagactag atagggacac ttctggcttg atgctctttg
    10021 ccaagcacgg ttatgcccat gcacgattag acaagcagtt gcagaagaaa tctatcgaga
    10081 aacgctactt tgctttggtt aagggagatg gacatttgga gccagaaggg gaaattattg
    10141 ctccgattgc gcgtgatgaa gattccatta ttaccagacg agtggctaaa ggcggaaagt
    10201 atgcccatac ttcatacaag attgtagctt cttatggaaa tattcacttg gtctatattc
    10261 acctgcacac tggtcgaacc catcaaatcc gagtccattt ttctcatatc ggttttcctt
    10321 tgctgggaga tgatttgtat ggtggtagtc tggaagatgg tattcaacgt caggctctgc
    10381 attgccatta cctatccttt tatcatccat ttttagagca agacttgcag ttagaaagtc
    10441 ccttgccgga tgatttcagt aaccttatta cccagttatc aactaatact ctataaaaac
    10501 tgtctcagag tataattatt atcttaaagg agaaaactca tggaagtttt tgaaagtctc
    10561 aaagccaacc ttgttggtaa aaatgctcgt atcgttctcc ctgaagggga agagcctcgt
    10621 attcttcaag caacaaaacg cttagtaaaa gaaacagaag tgattcctgt tttgcttgga
    10681 aatcctgaaa aaattaaaat ttatcttgaa attgaaggaa tcatggatgg ttatgaggtc
    10741 atcgaccctc aacattatcc tcaatttgaa gaaatggttt ctgccttggt ggagcgtcgc
    10801 aagggcaaaa tgactgaaga agatgtacgc aaggttttgg ttgaagatgt caactacttt
    10861 ggtgtgatgt tggtttactt gggcttggtt gatggaatgg tgtcaggagc gattcactca
    10921 acagcttcaa cagttcgtcc agctctacaa atcatcaaaa ctcgtccaaa tgtaactcgt
    10981 acttcaggag ccttcctcat ggttcgtggt acggaacgtt acctatttgg agactgtgcc
    11041 attaacatca atccagatgc agaagccttg gctgaaattg ccatcaactc agcaatcaca
    11101 gctaagatgt ttggcatcga acctaaaatt gccatgttga gctattctac taaaggttca
    11161 gggtttggtg aaagcgttga taaggtcgtt gaagcaacta aaattgctca cgacttgcgt
    11221 cctgaccttg aaatcgatgg tgagttgcaa tttgatgcag cctttgttcc tgaaactgca
    11281 gctctgaaag ctcctggaag tacggtagct ggtcaagcaa atgtcttcat cttcccaggt
    11341 atcgaggccg gaaatatcgg ttacaagatg gctgaacgcc tgggtggctt tgcggctgta
    11401 ggacctgttt tgcaaggttt aaacaagcca gttaatgatc tttctcgtgg atgtaatgca
    11461 gatgatgttt acaagttgac cctcatcaca gcagctcaag cagttcatca atagtgaaaa
    11521 ctataaagcg atatactatg ctatactgta gttatgaaac tatgtacgaa aagcactgcc
    11581 attaattcct gagaactaaa ttactgattg gtgtcaaaaa ggaaaacttc caagcgatga
    11641 tatcctgtct atacacgacc tatagaaatc tgtaatatgt atgtccgtaa aacgataatt
    11701 tccctttttg attttaaatg agtatgaaaa gagaattttt tcatgagaaa acgcataata
    11761 ttaagtgtct tgacacctgc tattatgcgt ttt
//
LOCUS       DAVBCW010000056        11601 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVBCW010000056 DAVBCW010000000
VERSION     DAVBCW010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11601
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.056"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            425..1135
                     /gene="deoD"
                     /locus_tag="VQ514_001313"
     CDS             425..1135
                     /gene="deoD"
                     /locus_tag="VQ514_001313"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HET2500065.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLDDAVCFNEVRN
                     MFGYTGTYKGHCVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKKLGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(1158..1682)
                     /locus_tag="VQ514_001314"
     CDS             complement(1158..1682)
                     /locus_tag="VQ514_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HET2500066.1"
                     /translation="MKIAIIGYSGSGKSTIAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFSPLTCLFRAFKRYL
                     KYRGKVRESMAADCPERFEWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(1735..1971)
                     /locus_tag="VQ514_001315"
     CDS             complement(1735..1971)
                     /locus_tag="VQ514_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HET2500067.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLAK"
     gene            complement(2039..2959)
                     /locus_tag="VQ514_001316"
     CDS             complement(2039..2959)
                     /locus_tag="VQ514_001316"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HET2500068.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEKKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWISINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            3149..3553
                     /locus_tag="VQ514_001317"
     CDS             3149..3553
                     /locus_tag="VQ514_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500069.1"
                     /translation="MKILKRYILELCFILSFALPFIKGTNADNGRCFVETYYGFTFLM
                     EHAIVTAVFIYSFLIAFLLKKRWAKWIAAGSYCFLVLWIATEGYFFRMSLEDLIRLWT
                     SLEILTQTYQLGFYLNILLGILLIIKYFKVKQ"
     gene            3769..4350
                     /locus_tag="VQ514_001318"
     CDS             3769..4350
                     /locus_tag="VQ514_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET2500070.1"
                     /translation="MYYAENPDAAHDIHELRVDLLGQKMTFLTDAGVFSKKMVDFGSQ
                     LLLKCLDVNQGETVLDVGCGYGPLGLSLVKVYGVQATMVDINTRALDLARRNAEKNNA
                     KATIFQSNIYEQVEGHFDHVISNPPIRAGKQVVHEIIEKSKDFLETGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            4347..5624
                     /locus_tag="VQ514_001319"
     CDS             4347..5624
                     /locus_tag="VQ514_001319"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HET2500071.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLVEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIRGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVHQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            5642..6304
                     /gene="deoC"
                     /locus_tag="VQ514_001320"
     CDS             5642..6304
                     /gene="deoC"
                     /locus_tag="VQ514_001320"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HET2500072.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKIVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVTLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            6291..6680
                     /locus_tag="VQ514_001321"
     CDS             6291..6680
                     /locus_tag="VQ514_001321"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET2500073.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMAEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            6770..7822
                     /locus_tag="VQ514_001322"
     CDS             6770..7822
                     /locus_tag="VQ514_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HET2500074.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALNNAVKDAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            7966..9501
                     /locus_tag="VQ514_001323"
     CDS             7966..9501
                     /locus_tag="VQ514_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2500075.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            9494..10552
                     /locus_tag="VQ514_001324"
     CDS             9494..10552
                     /locus_tag="VQ514_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2500076.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            10555..11511
                     /locus_tag="VQ514_001325"
     CDS             10555..11511
                     /locus_tag="VQ514_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2500077.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGIIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
BASE COUNT         3299 a         2066 c         2657 g         3579 t
ORIGIN      
        1 ggtttgaaga gattttcgaa gagtataaac agaaaggtag agcgcgtgtt ctaatttgaa
       61 cacgagtaga aaacttttct aaaaacaaaa acgaaaggat gggtaaactg tattcgctga
      121 actgaatacg ggcgactctc ctctaaatca aaattaagaa aggaattgac cccaccctaa
      181 aagtagtggg aaaaagatag ttggtctagc gagcatcgct cactgcgccc aactcctatt
      241 ttcccttcgc tttttgatgg gtttggtatc tttctcaata taaaataaag aaaggtagag
      301 cgtgtgtttt gatttgaaca cgagcggaaa actcggaaaa tagataatct gactgaaaaa
      361 tcaggatttc tcgtcaggtt cctaattttc agtcgttttc ttctcgctct ttgtatcata
      421 aattatgtct atccatattg ctgctcagca gggtgaaatt gctgataaaa ttcttcttcc
      481 tggggatcct cttcgtgcta agtttattgc ggagaatttc cttgatgatg ctgtttgttt
      541 taacgaagtg cgtaacatgt ttggttacac tggtacttac aagggtcact gtgtatctgt
      601 catgggaact gggatgggaa tgccatctat ttcgatttat gcgcgtgagt taatcgtaga
      661 ctacggtgtg aagaaattga ttcgtgtggg aactgcaggt tctttgaatg aagaggttca
      721 tgttcgtgaa ttagttttgg cgcaggcggc tgcaaccaac tcaaacatcg ttcgtaatga
      781 ctggccacag tacgattttc cacaaattgc tagctttgat ttgcttgata aagcctacca
      841 tatcgccaaa aaacttggta tgactactca cgttgggaac gttttgtcat ctgatgtctt
      901 ttactcaaat tactttgaaa agaatatcga gcttggtaaa tggggagtca aggctgtgga
      961 aatggaagca gcagctcttt actatcttgc tgcccaatac catgttgatg cgctagctat
     1021 catgaccatc tctgatagct tggtcaatcc agacgaagac acaactgcag aagaacgtca
     1081 aaataccttc actgatatga tgaaggttgg tttggaaacc ttgattgcag aataattata
     1141 gccaaaaagg aagcccttta tgaattgtag gacttccttt tcttatccag aaattgatct
     1201 agctctctct gatttcgaag aatagtgact ttatgtgaat attcttggca aagtttttgg
     1261 taattttctt tttgagtttt gctacgccca tcccaaagaa tccatctgat aaactcccac
     1321 tcaaagcgtt cagggcaatc tgccgccata ctttctctga cttttccacg gtatttaaga
     1381 taacgcttaa aggctctaaa gagacaggtc aatggcgaaa aattgagaaa gatgatttgg
     1441 tcagcttctt gcattcgttc ttggtagtag caccaagaat aattaccatc gatgacccaa
     1501 gctttatgct tggtgagaaa gttttttatc tcggttaaca tccattcgca gtcactgtct
     1561 tgccaaccag gttgaaattg gagtgtgtcc atgtgcagtt ttggaatgga gtagtagtta
     1621 gataactttt ctgctatagt tgacttacca gaaccagaat atccgataat tgcgattttc
     1681 attttctact ttttcctatt tggagacaaa aaaacagcct ctatggactg tttcttattt
     1741 agcaagttta gctgaaagac gagctttatc gcggcttgct ttgtttttgt gaatcaaacc
     1801 tttagtttct gctttatcga tagctgagct agcagcacgg aaaagttctt cagatgggtt
     1861 tgcttcgaaa gcttttatag cagtacgcat agctgatttt tgagctgagt tcttttcgtt
     1921 ttgtttaacg ttcaattcag cgcgtttgat agctgattta atgtttgcca atgttcttac
     1981 ctccatattt actaactata ccattatata tgaaaactta cgttttgaca agggaaaatt
     2041 acttttttaa gtaaatttca tcgatttcat ggttcttgct tttatgaaga atcacttctg
     2101 cacgatttct ggttggttca atataatttt gcagatttgt gagattgata ctgatccaga
     2161 cctgatgggc aaaggactcc acttccccaa tcggcatctg agtaaaacga taatagtagc
     2221 tatcagggtc gttttgggct aggctcagca ttttcaagaa acggtccaga taccaacttt
     2281 caatatcatc cactccagca tctacataga tggaaaagtc aaagaagtca gtgatataga
     2341 gacgatcgtt ttgtggattt tgaaagacat taattccctc aacaattaca aaatcagcag
     2401 ctttgacact ttgtttcttt tcgggtacga tgtcgtaaac ttcatgagaa tagacaggaa
     2461 tatctacatc ttgtccattt ttgatgcggt ccaagaagtt gagaagagct tccatatcat
     2521 agctttcagg aaatccttta cgatttaaaa tcccctgctc aatcaaggtt tgattgggat
     2581 agagaaaacc atcagttgta accaactcaa ccgtagcatc tgtaaacgta cgggacagta
     2641 ggatttgaag taggcgactg gttgtggatt ttccaacggc aacactccca gaaaccccaa
     2701 taataaaagg ttgagattta ctttcacgtt ggaggaaaat tccttttgaa aaggctaaat
     2761 cttccttagt tcgcttgtaa atctgaatca gatgagccaa ggggagatag atatctgtaa
     2821 cgtcttggag actgatttgg tcattaaaac tcttgataga ttccaattct tcttctgtca
     2881 aaggaggtgt tgtctttcga tgtaaagatt gccaagtctg gcggctgatt ttttcaaaat
     2941 gtaaaaattc gttggtcatt tgttttcccc tagatatttt gtctatcata ccataaaaag
     3001 ctaaaaaaat aaagcggttt cttgaatgtg gtaagtcaat ggtatatagt ataagtgatc
     3061 aatttattga tgaaatagga gatagattct actgatttat aagcttataa agggggcttt
     3121 ttagttcaca acttatagga ggtgtactat gaaaatctta aaacgttaca tattggaact
     3181 ctgttttatt ttaagttttg ctttaccttt tataaaagga accaatgcag ataatggtag
     3241 atgctttgtg gaaacctatt acggttttac ttttttgatg gaacatgcta ttgtaacagc
     3301 tgtctttatc tattcgtttt taattgcttt cttactaaaa aaacgatggg cgaaatggat
     3361 tgctgctggt agttattgct ttttagtcct atggattgcg acagagggtt atttcttccg
     3421 catgtcacta gaagatttga tacgactttg gacaagtttg gaaatcctga cacaaacgta
     3481 tcagttgggc ttttatctaa acattttgtt aggaattttg ttgataataa agtattttaa
     3541 ggttaagcaa tagaaataat aatctgcctg attatagatg ataaactttt tgtttttatc
     3601 tggactgtga tatttatagt tctaaatggt ttattattta taagtgttga aagagattga
     3661 agaaaatggc ttcgtgactt gctttcttcg gtctttttcg ttttattatc aatttgtaaa
     3721 cgatttacaa tttgagtcaa attatggtaa aatagtgtca tgagtaaaat gtattatgca
     3781 gaaaatcctg acgctgctca cgacattcat gagttgagag tggacttgtt gggacaaaaa
     3841 atgacttttt tgacagatgc gggtgttttt agcaagaaaa tggttgactt tggaagtcaa
     3901 ctcttgctca agtgtctgga tgtcaaccaa ggagagacag tcctagatgt aggctgtggt
     3961 tatggtccat taggtttgtc cttggtcaag gtttacggag ttcaggcgac catggttgat
     4021 attaacactc gtgccttgga tttagctcgg agaaatgctg aaaaaaataa tgcaaaagcg
     4081 acgattttcc agtccaacat ctatgaacaa gttgaaggac attttgatca tgtcatttcc
     4141 aatcctccta tccgtgcggg caagcaagtc gttcatgaaa tcattgagaa aagtaaagat
     4201 ttcttggaaa ctggtgggga tttgacaatc gtcatccaga aaaaacaagg ggctccaagt
     4261 gctaagtcca agatggagga tgtgtttggt aattgtgaaa tccttaaaaa agacaaagga
     4321 tactatatcc ttagaagtgt gaaagaatga gagcagttga tttaatacaa aaaaaacgag
     4381 acggtcaaga actgacttca agtgaaattg aatggctggt agaaggctat gtgtcaggaa
     4441 ctgttcctga ttatcagatg tctgcctttg ctatggctgt ttattttaaa ggaatgacga
     4501 ctcgtgaaat ttctgattta acgatgaata tggttaagac tgggcaagag tttgacctgt
     4561 cagccattga tggtgtgaaa gttgacaagc attctactgg tggtgtcggc gataaggtaa
     4621 ccttgatttt agctcctctt gttgctagct tcggtgtgcc tgtagcaaaa atgagtggtc
     4681 gtggtcttgg ccatacaggc ggaacaattg ataaattgga gtccattagg ggctatcaag
     4741 tagaacgtag tcaagaggat tttattcgtc aggttcagga tattggagta tctgtcattg
     4801 ggcaatcaga ccagctggtc aaagcagata agcttctcta tgcccttcgt gatgtgaccg
     4861 caactgtcga cacgattcct ttgattgcga gttcggtgat gagcaagaaa attgcggcag
     4921 gggcggatgc tattttgcta gacgtgactg tcggtgaggg tgccttcatg aagacggttg
     4981 atgaggcgcg tgagttggct caaactatgg tagatcttgg taaggttgtt ggacggaaga
     5041 cagtagcagt gattacggat atgagccagc ctttgggacg agcgattgga aatcgtctgg
     5101 aaatacttga agcattggag attttgcaag gtcaaggacg tcaggatatt acccacttta
     5161 tttgtgaatt ggctcaaatt atgcttggtt tggcaaatgt taacaagact gttgaagaag
     5221 ttcaccaaca tcttgagaat ggtcaagcac tggctaagtt tgaggaaatg gtgcaagccc
     5281 aaggtggaga cttggaagac ctctatcgtc ctgtaaatgt agctcatgtg gtggaaatcc
     5341 ctgctcagga aacgggtgtc atttcagctc ttccagctat ggactttggt ctttatgcca
     5401 tgaggctggg ggctggtcgt gcagttaagt ctgatgcctt ggactatgaa acaggaattg
     5461 tttttgaaaa gaagattgga gactccgttc aaaagggtga aattgttgca aaagtataca
     5521 caaatgaaaa aattccacct caactagtta cagatttcca aaaatgtgtt aaaataagtg
     5581 atgaagtgaa aaaaatccgt gaaattgtag aaatcatctc ctaaatggag ggaaaaccga
     5641 aatgaaatta aataaatata tagatcatac gcttttaaaa caagatgcaa agaaaaaaca
     5701 aattgatagt ttgttgtctg aggctagaga gtatgacttt gccagtgttt gcgttaatcc
     5761 gacctgggtt gaacatgcta aaaaaggact tgaaggcaca gatgttaagg tttgcacggt
     5821 agtaggtttc cctttgggag caacaacttc agccgtgaaa gcatttgaga caaaagaagc
     5881 tatccaaaat ggtgcagatg agattgatat ggtgatcaat gttggagctc tcaaatcagg
     5941 taatttagcc ttggttgagt cagatattcg cgcagtagtg gaagcaagtg gtgataagtt
     6001 agtgaaagtc attattgaag cttgccttct gacagaccaa gaaaaaattg ttgtttgcca
     6061 attggcccaa aaagctgggg ctgactttgt taaaacatct actggttttt caactggtgg
     6121 tgctacgata gcagatgtta cattaatgcg tgaaacagtt ggatctgata tgggagtcaa
     6181 ggctgctggt ggagctcgtt cttatgcaga tgctcttgcc tttgtcgaag caggtgcgac
     6241 ccgtatcgga acgtcagctg gggtagctat tttaaaagga gaattggcag atggcgacta
     6301 ctgagttgat tgaactggca attgaaacca gcaaacatgc ctatgtcccc tattctcact
     6361 ttcctatcgg agcagtttta gtagccaaag acggaagtgt ttacacggga gtgaatatcg
     6421 aaaatgctag ctatcctttg accaattgcg gtgagagaac agctattttt aaggctatat
     6481 ctgaaggcca aagagaattt tcagaattga ttgtctatgg tcaaactgaa aaaccgatct
     6541 cgccatgtgg tgcttgtcgc caagtgatgg ctgaattttt tgaacaagat ctaaaagtga
     6601 ccttagtcgc aaaagataaa tcgacggtcg agatgacggt cggggaatta cttccatact
     6661 cttttacaga cttaaactag tctgagtcgc tctctgagtg gcacggtcct tgtggccaac
     6721 caatccatac ttgcaacatc gttgcacatc ttatttagga ggttcagtaa tgaacaagaa
     6781 acaatggcta ggtcttggcc tagttgcagt ggcagcagtt ggacttgctg catgtggtaa
     6841 ccgctcttct cgtaacgcag cttcatcttc tgatgtgaag acaaaagcag caatcgtcac
     6901 tgatactggt ggtgttgatg acaaatcatt caaccaatca gcttgggaag gtttgcaggc
     6961 ttggggtaaa gaacacaatc tttcaaaaga taacggtttc acttacttcc aatcaacaag
     7021 tgaagctgac tacgctaaca acttgcaaca agcggctgga agttacaacc taatcttcgg
     7081 tgttggtttt gcccttaata atgcagttaa agatgcagca aaagaacaca ctgacttgaa
     7141 ctatgtcttg attgatgatg tgattaaaga ccaaaagaat gttgcgagcg taactttcgc
     7201 tgataatgag tcaggttacc ttgcaggtgt ggctgcagca aaaacaacta agacaaaaca
     7261 agttggtttt gtaggtggta tcgaatctga agttatctct cgttttgaag caggattcaa
     7321 ggctggtgtt gcgtcagtag acccatctat caaagtccaa gttgactacg ctggttcatt
     7381 tggtgatgcg gctaaaggta aaacaattgc agccgcacaa tacgcagccg gtgcagatat
     7441 tgtttaccaa gtagctggtg gtacaggtgc aggtgtcttt gcagaggcaa aatctctcaa
     7501 cgaaagccgt cctgaaaatg aaaaagtttg ggttatcggt gttgatcgtg accaagaagc
     7561 agaaggtaaa tacacttcta aagatggcaa agaatcaaac tttgttcttg tatctacttt
     7621 gaaacaagtt ggtacaactg taaaagatat ttctaacaag gcagaaaaag gagaattccc
     7681 tggcggtcaa gtgatcgttt actcattgaa ggataaaggg gttgacttgg cagtaacaaa
     7741 cctttcagaa gaaggtaaaa aagctgtcga agatgcaaaa gctaaaatcc ttgatggaag
     7801 cgtaaaagtt cctgaaaaat aatggatgga agtcatttct taggaagcgg ccaagggccg
     7861 cttttttaag agttgagaca aagtcatttt gtctcagtaa agcttgctac tagacaaact
     7921 agcaagtttt attgaaataa aataagactc tgaaaggaag agcacatggc acacgaaaat
     7981 gtcattgaga tgcgtgatat taccaaggtg tttggtggat ttgttgccaa cgacaaaatc
     8041 aacttgcacc tacgaaaagg tgaaattcat gcacttttag gagaaaatgg ggctggtaag
     8101 tccacgctaa tgaacatgtt agcaggcctt cttgaaccaa ctagtggtga aatcgcggtc
     8161 aacggtcaag ttgtcaatct cgactcccca tctaaagcag ctagcttggg aatcgggatg
     8221 gttcaccagc actttatgtt ggttgaagcc ttcacagtgg ctgaaaacat cattttaggt
     8281 agtgaattga ctaaaaatgg tgtgctagat atcgctggag ctagcaaaga aatcaaggct
     8341 ctttctgaac gttatggctt agctgttgac ccttctgcca aggtagcaga tatctcagtt
     8401 ggagcccaac aacgtgtaga aattttaaaa acactttatc ggggggctga tatccttatc
     8461 tttgacgaac caacggctgt tttgactcca tcagaaattg atgagttgat ggctattatg
     8521 aaaaatcttg tcaaagaagg aaaatcaatt atcttgatta cccacaaatt ggatgaaatt
     8581 cgcgcagttt ctgaccgtgt tacagttatc cgtcgtggga aatcaattga aaccgttgaa
     8641 attgcagggg ctaccaatgc tgatttggcg gaaatgatgg taggacgttc tgtttccttt
     8701 aaaacagaga agcaagcctc taaaccaaaa gaagtggttt tgtctatcaa agatttggtg
     8761 gtcaatgaaa accgtggtgt tccagctgtt aaaaatctgt ccttggatgt tcgtgctgga
     8821 gagattgttg gtattgcggg gattgatgga aatggtcagt ctgaactgat tcaagccatt
     8881 acaggtcttc gtaaggttga atctggtagc attgagctaa aaggagattc aattgtaggc
     8941 ttgcacccac gtcagattac agaactaagt gttgggcacg ttccagaaga ccgtcaccgt
     9001 gatggcttga ttttggaaat gatgatatct gaaaatattg cccttcaaac ctactataaa
     9061 gaaccacata gtaaaaatgg aattttgaat tattcaaata ttacttctta tgctaaaaag
     9121 ctgatggaag agtttgatgt tcgcgctgcc agtgaattag ttcctgcagc tgcactctca
     9181 ggaggaaatc aacaaaaagc aattattgct cgtgaaattg atcgagatcc tgatctcctt
     9241 atcgttagcc agccaactcg tgggttggat gtcggtgcca ttgagtatat ccacaaacgc
     9301 ttgattgaag agcgtgataa tggcaaggct gtccttgttg tcagctttga attggatgag
     9361 attttaaacg tctcagaccg tattgccgtt atccacgatg gtaagattca aggtattgta
     9421 tcaccagaaa caaccaataa acaagaactt ggtgtcttga tggctggtgg aaacttggga
     9481 aaggagaaga gtgatgtcta aaaaattaca acaaatttcg gttcccttga tttctgtatt
     9541 tctaggaatt ttactcggag ccattgtcat gtggatcttc ggttatgatg ctatttgggg
     9601 ctacgaagaa ttgttctata cagcctttgg cagtctgcgt gggattggag aaatcttccg
     9661 tgctatgggt cctctggtct tgattggtct tggttttgcg gttgccagtc gagctggttt
     9721 ctttaacgtc ggacttcctg gtcaggcttt ggcaggttgg attctcagtg gttggtttgc
     9781 gctgtcgcat ccagatatgc cccgtccctt gatgattcta gcaaccatcg tgattgcctt
     9841 gattgctggt gggattgtcg gagcgattcc aggtattctt agggcctatc tagggacgtc
     9901 agaggttatt gtaaccatca tgatgaacta cattgtcttg tatgtaggga atgcctttat
     9961 ccatgctttc cctaaagact tcatgcaaag tacagattcg accattcgtg ttggggctaa
    10021 tgcaacctat cagacacctt ggttggctga gttgactggt aactcacgga tgaatattgg
    10081 tattttcttt gccatcattg ccgttgcagt tatttggttc atgctcaaga aaacaactct
    10141 tggttttgaa atccgtgcag ttggtcttaa tccacatgct tcagaatatg ctggtatttc
    10201 tgccaagcgg actattatcc tatctatgat tatttcaggt gccttggcag gtcttggtgg
    10261 agctgttgaa ggtttgggaa ccttccagaa cgtctatgtt caaggttcgt cattagctat
    10321 cggatttaac ggaatggcgg ttagtttgct tgcggccaac tcaccaattg gtatactctt
    10381 tgcagccttc ctatttggcg ttctccaagt tggggctcct ggtatgaatg cggcgcaggt
    10441 accatctgag cttgtcagca ttgtaacagc gtctattatc ttctttgtca gtgttcatta
    10501 ccttatcgaa cgctttgtca aaccgaaaaa acaagttaaa ggaggtaagt aaagatgtct
    10561 attataacct tgctccccct cttggtgtct tctatgctga tttactcagc acctctcatc
    10621 tttacaagta tcggtggtgt tttctctgaa cgtggtggtg tggtaaacgt tggccttgaa
    10681 ggaattatgg ttatgggtgc cttttctgga gttgtcttta accttgaatt tgcagaacaa
    10741 tttggagcag caactccatg gctatccttg cttgtagcag gattggttgg tagtgttttc
    10801 tctatcatcc acgcagcagc gacggttcat ttccgtgcag accatgttgt cagcggtacg
    10861 gtattgaact tgatggcgcc tgccttggct gttttcttag ttaaagttct ttataacaaa
    10921 ggacaaaccg acaacctaag tcaaactttt ggacgctttg atttcccagt cttggcaaat
    10981 attccagtga ttggtgatat cttcttcaag tcaactagtc tacttggtta tctggcgatt
    11041 gccttctcat tccttgcttg gtttattctc tttaagactc aatttggtct tcgtctccgc
    11101 tctgtcggtg aacatcctca agcagcggat accttgggaa tcaacgtcta caagatgaga
    11161 tatttaggga ttattatttc aggttttcta ggtggaattg gcggagcgat ttatgcgcaa
    11221 tcaatctcag ttaacttctc agtgacaact attgttggac ctggatttat cgcccttgct
    11281 gcgatgatct ttgggaaatg gaatccaatc ggtgctatgc tttctagtct cttctttgga
    11341 ctttcacaaa gtttggctgt tatcggttct caattgccgt tcctacaagg agtgccagcg
    11401 gtttatcttc aaattgcacc ttatgttttg acaattcttg tcttagcagc cttctttgga
    11461 aaagcagtcg caccaaaagc agatggtatc aactacatca aatcaaaata agcatacaaa
    11521 aaaacgtcag tccaatgact gaactgcccc ccaaaagtta gacagaaaaa atctaacttt
    11581 tggggtgttt ttattatgaa a
//
LOCUS       DAVBCW010000057        11593 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVBCW010000057 DAVBCW010000000
VERSION     DAVBCW010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11593
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.057"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(<1..1163)
                     /locus_tag="VQ514_001326"
     CDS             complement(<1..1163)
                     /locus_tag="VQ514_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HET2500078.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMKELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     KGEILEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESSEKFEAIKQNLVDEMWKVAQRELATGFYYGTPSENEQLFGARRKIPEYKFVAEVV
                     SYDDATQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNP"
     gene            1787..1969
                     /locus_tag="VQ514_001327"
     CDS             1787..1969
                     /locus_tag="VQ514_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HET2500079.1"
                     /translation="MANDNKSHYLIYRVLGISFEEGENIDLYQNKGRFLYKYAGSFLE
                     EAAVLSFNEKFGTENT"
     gene            1979..2344
                     /locus_tag="VQ514_001328"
     CDS             1979..2344
                     /locus_tag="VQ514_001328"
                     /inference="COORDINATES: protein motif:HMM:NF021048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HET2500080.1"
                     /translation="MPNSENTKPKTFEIDCLVGEKHAYEIKWWDATTDGDHITKEHTR
                     IKVIHNKGYIPIRLMFYYPNRTQAIKIQQTLETLYNGIGGKYYYGDSAWEHLRAVTGI
                     DLLSILTDIANKKTGVKSK"
     gene            2341..3531
                     /locus_tag="VQ514_001329"
     CDS             2341..3531
                     /locus_tag="VQ514_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HET2500081.1"
                     /translation="MTVLKGDNLEILKTIESSSIDLIYMDPPFFTQKTQKLSNNKNIM
                     YSFEDTWTSIEDYKEFLSVRLEECKRVLKNSGSIFVHCDKIANHHIRLILDNIFGADM
                     FQSEIIWNYKRWSNSKKGLLNNHQNIYFYSKSKDFKFNTIFTEYSSTTNIDQILVERK
                     RNGNSKTIYKVDNNGNYILAKEKNGVPLSDVWNIPFLNPKAKERVGYPTQKPILLLEQ
                     IIKIATDKNDIVLDPFCGSGTTLVASKILNRNYMGIDLSEEAINITQQRLENVIKTSS
                     NLLNKGIEAYRTKTEEEENILKLLQAKIVQRNKGIDGFLPKHFQKKPIPIKIQKNNEC
                     LNESISLLQNAINSKKLDFGVVIKTHSDNLLFDFDTIPENIIVVDHFELTIEKWLSKS
                     QQLL"
     gene            complement(4046..5038)
                     /locus_tag="VQ514_001330"
     CDS             complement(4046..5038)
                     /locus_tag="VQ514_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2500082.1"
                     /translation="MSYYDFVKDYMKVDECKNNEKYSSAGHTFCWKKENSTYAEGLYW
                     FYEGGSFIIDIHDFYIKEEVVQNSTYSMSDYVSIYSSYIVSANGEKFNPYQTITANSL
                     CTFDFDNIKDDFVFLLHENCCYLAVSIGFKKELIEKHLASININPESFYSTLLQPNQI
                     ILTKALERVAMEILNCKMDAPAADFFFKAKANEWISIVIDTYLNRKKYKIEIDDDKAL
                     EDVARFLDDHFATNVNQGTLEKISKMSGTKLKNLFKEKYCQSITEYTQRKRMNVAETL
                     LLNTELPIKEIAESVGYSSASKFSIYYKRYKGKLPSEVRNLACKHHNFKCDYCD"
     gene            5198..6952
                     /locus_tag="VQ514_001331"
     CDS             5198..6952
                     /locus_tag="VQ514_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2500083.1"
                     /translation="MKIYKKLFAYVQDKKYLGVLAIIFSAISAVLTVYGYYLIYKFLD
                     KLIINSNLSGAESIALKSVITLTSGAIFYFVSGMFSHILGFRLETNLRKRGIDGLEKA
                     SFRFFDLNPSGQIRKIIDDNAAQTHQVVAHMIPDSSQAIVTPILVLVLGFIVSIRVGI
                     ILLALTIIGGLILGAMMGEQEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFK
                     SFYKAIKDYSKYAYDYSLSCKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAEVILLE
                     LIMILFLSGVLFVSFMRMMWYSMYISQGNYAVDTLEALYEDMQKDKLVHGNVNNFKNY
                     NIEFENVSFAYNDKAIIENLSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNKGSI
                     KIGGIAISEYSDEALIKAISFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGC
                     DLILDKFPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEF
                     ELQKAFKNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSL
                     QEMFNSANEWRVSNERVL"
     gene            6936..8657
                     /locus_tag="VQ514_001332"
     CDS             6936..8657
                     /locus_tag="VQ514_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2500084.1"
                     /translation="MKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAILLMIFA
                     QEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTAENLSKL
                     PLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAGNVKMGL
                     AVIIPSILSFIFIPLSKKYQVNGQNRYYDVLRKNSESFQENIEMQMEIKAYNLSKDIK
                     DDLYKKMEDSERIHLKAEVTTILTLSISSIFSFISLAVVIFVGVNLIINKEINSLYLI
                     GYLLAAMKIKDSLDASKEGLMEIFYLSPKIERLKEIQNQDLQEGDDYSLKKFDIDLKD
                     VEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYDYDKGQILIDGK
                     DIKEISTESLFDKVSIVFQDVVLFNQSVMENIRIGKQDASDEEVKRAAKLANCTDFIE
                     KMDKGFDTVIGENGAELSGGERQRLSIARAFLKDAPILILDEITASLDVNNEKKIQES
                     LNNLVKDKTVVIISHRMKSIENADKIVVLQNGRVESEGKHEELLQKSKIYKNLIEKTK
                     MAEEFIY"
     gene            8668..9297
                     /locus_tag="VQ514_001333"
     CDS             8668..9297
                     /locus_tag="VQ514_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HET2500085.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMALIALGAFLGGILGAYIGKALLKKHFSNGLYCVGYFTPCLILWCYLN"
     gene            9198..9755
                     /locus_tag="VQ514_001334"
     CDS             9198..9755
                     /locus_tag="VQ514_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HET2500086.1"
                     /translation="MYRQGSIEKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIP
                     IVIRMFILPFMAASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKN
                     IKMAMRVRGINFKNPVKYLEYVSVPLLIISSNISDDIAKAAETKAIENPIAKTRYIRV
                     KIQLIDFVYVLAVAGLIVGGLIWLK"
     gene            9743..11107
                     /locus_tag="VQ514_001335"
     CDS             9743..11107
                     /locus_tag="VQ514_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2500087.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGSGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDILEIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDESSSNLDIKSISVLAKMLKILKEKGISIIVAEHRIYYLMDIVDRVFLIDKGKLK
                     KTYTRSEFLKLDKNELNALSLRDKELSKLKVPYLKEGGEYQIKNLSYKFTDDECLSLK
                     DISFKLGKIYGIIGSNGRGKSTLLRCLIGLEKKSKEEIYFKGEKLSKKERLKNSSLVM
                     QDVNHQLFTDEVFNELRLGVKNFDEEKAKIILKDLGLDEFIERHPMSLSGGQKQRLAI
                     ASVMCKTSPFIFFDEPSSGIDYSNMIKISELINKYKTMDKIIFIVSHDIEFLNEVADE
                     IFEL"
BASE COUNT         4213 a         1666 c         1968 g         3746 t
ORIGIN      
        1 ggatttggag cgcggtcgat tttattgcct ttggcatcat gcaaatcttc aatataggtt
       61 tcaaaatgac ggaaacctgg accataaaac tcaacttggt ccccttcgtt aatgacgttt
      121 cgttgacgaa tagttgctgt ttgtgtcgca tcatcataag aaaccacttc agcgacaaac
      181 ttgtactcag gaattttacg acgagcacca aacaactgct cattttcaga tggtgtacca
      241 tagtaaaatc ctgtagccag ttcacgttgg gcaaccttcc acatctcgtc cactaagttt
      301 tgtttgatgg cttcaaactt ttcagaactt tcaagatagg catccacagc cgccttgtag
      361 cagttggtta ctgttgatac gtagtgaata gacttcatac gtccttcgat ttttagactg
      421 tccacaccat tttcaatcat atctggaatg tggtcaatca tagacatatc aacggctgac
      481 attgaaaatt cttctagaat ctcacccttc aaactcttac gttctttccc aaatggcata
      541 tcgtaaaggt cgtatttcca acggcatgac tgagaacacc caccacggtt ggcatcacgc
      601 atactcatgt ggtttgaaag agtacaacgt ccagagtatg aaatacacat agctccatgg
      661 acaaaggctt caatttcaac atctgtacgt ttgcggattt cagctaattc tttcattgaa
      721 acctcacgtg ctaaaacgac acgagtcaag cctagctctt tccagaactc aagggtttca
      781 tagttagtgg cactggcttg ggtagaaagg tggatttcaa ggcctggtgc ttcagtcgct
      841 gcaatcataa tcaaggctgg gtcagatacg ataactgctg caatcccgat atcacgcagt
      901 ttacgaaacc actcaccagc accagcttca tttccttcgt gcataaccat attagccgct
      961 acatagacct tggcaccata cttggccgca aactgcacgc cttcttccat ctgttcgaaa
     1021 gtaaagtttc ccgcacggct acgaagacca taggcctgac caccgataaa gacagcatct
     1081 gctccatact gaacagctac ctttagcttc tctaaagtcc ctgcaggtga taaaacctca
     1141 ggacgtttta atgtttttgt catgatttct cctatttgca atataaaagt ttgacatttt
     1201 tccttctcat tttatagtaa ataaaggata aaatcaagcc tagacggatt gttttgacaa
     1261 ttctaatctt ttagactttg cataataact attaaacagt cgaaaattac cccagtttta
     1321 acaagtagtc aaaaagtcta aaaaagtaaa aaccagtatc ccaagatact gactcgtaaa
     1381 aatatataaa ttagtcctta gatgactact cccactctgt tgctcctata atggaaaagg
     1441 attgatacag tctagaatat cgctttttta tttattattc tgatataaaa ctgttagaat
     1501 tgattttttg ttttataccc aaatatgaat agagacgtag ctaataagtt gaataacata
     1561 tttggaaaaa tgagaaatag caatattact agaactttta gaaacaatca atatttaaaa
     1621 agaaaaaagc tcaaagaatt tattaaacca tgttataata gatatagcta taattttatt
     1681 tataacaaga ggatagaaat gaccgaatta gaaagaaaaa atcgaaaaat tagctaagaa
     1741 atattctgat aacttaaaca tcaaagttca agagagagtt cgtgaaatgg caaatgataa
     1801 taagagccat tatttgatat acagagtttt aggtatttca tttgaagaag gagaaaatat
     1861 cgatttgtat caaaataaag gtcgtttttt atacaaatat gctggttcat ttttagaaga
     1921 agctgcagta ctatccttta acgaaaaatt tggtacagaa aatacttaaa aagttaacat
     1981 tcctaattct gaaaatacaa aacctaagac ttttgaaatt gattgtttag tcggagaaaa
     2041 acacgcatac gaaataaaat ggtgggatgc aactacagat ggagaccata taactaaaga
     2101 acacactaga ataaaagtta ttcataacaa aggatatata ccaattcggt taatgttcta
     2161 ctatccaaat agaactcaag ctataaaaat tcagcaaact ttagaaacat tgtataacgg
     2221 tattggaggg aaatattatt atggagattc tgcctgggaa catttaagag cagtgaccgg
     2281 tattgattta cttagtattc taacagatat tgcaaataaa aaaacagggg taaaatcaaa
     2341 atgacagtat taaaaggaga taacttagaa atattaaaaa ctattgaatc ctcaagtatt
     2401 gatttaatct atatggaccc tcctttcttt acacagaaaa cccaaaaatt atctaataac
     2461 aaaaatatta tgtattcatt cgaagatacg tggacttcga ttgaggatta caaagaattt
     2521 ttgtctgtaa gattagaaga atgcaaaaga gtgctaaaaa atagtggcag tattttcgtt
     2581 cattgtgata aaattgcaaa tcatcatatt agattaattt tagataatat ctttggagca
     2641 gatatgtttc aaagcgaaat tatatggaac tataaacggt ggtctaattc aaaaaaggga
     2701 ttattgaaca atcatcaaaa catttacttt tattcaaagt caaaagattt taaatttaat
     2761 acaattttta cagagtattc ttctactaca aatatcgacc aaatactagt agaacgaaaa
     2821 cgaaatggaa actctaaaac tatatataaa gttgataata atggtaacta tattctagca
     2881 aaagagaaaa atggagttcc cctttcagat gtttggaata taccatttct taatccaaaa
     2941 gctaaagaaa gagtaggtta tcctacacaa aaacctattc tgttattaga acaaattata
     3001 aagattgcta ctgataaaaa tgatatagtt ttagacccgt tctgtggaag tggaactact
     3061 ttagtagcct ccaagatttt gaatagaaat tatatgggga ttgatttatc tgaggaagct
     3121 atcaatataa ctcagcaacg tctggaaaat gttataaaaa caagttcaaa tttattgaat
     3181 aaaggaatcg aagcatatag aaccaaaact gaggaagagg aaaacattct taaattatta
     3241 caggcaaaaa ttgttcaaag aaataaagga attgatggtt ttttacctaa acattttcaa
     3301 aaaaaaccga tacctataaa aattcaaaaa aataatgaat gtctgaatga gagtatctct
     3361 ttattacaga atgctataaa ctccaaaaaa cttgattttg gagtagttat aaaaactcat
     3421 tcagataatt tattatttga ttttgatacg attcctgaaa atataatagt agttgaccat
     3481 tttgaactta caatcgaaaa atggttaagt aaaagtcaac agttactata gtttgcaaat
     3541 tatttacaga aagaacaaaa aatgctctga aacactgatt tcaaggcatt ttttgttagt
     3601 ttaaaattac tcccattctt cttttccaaa cgtacaatat atccaaaacc attcaaaata
     3661 ctagattcta ttttttataa tatcactaaa tccacctaat tataggacgt tttcagattt
     3721 ttagtcccgt cccagtccca gtaccggaga aatattgttt taatataata tctctttttg
     3781 tcttctaagc tcttaaaagc aaaagaacaa gtaaagagtc aagacaagga taaaaagtcc
     3841 atattagggc aaataaaaag ctttaagaca gatgacaaat ctaagtcaaa taagaaagac
     3901 catagcaaag gtgcagagag ataaatattg gcggtcttcg gactgccttt atttttttat
     3961 ccatttttca aatcaaattt attcagacta tatatgcaca tatacactta aattcatata
     4021 aaaacatggc ttgtaaaaaa ttactttaat cacaataatc gcatttaaaa ttgtgatgtt
     4081 tgcaagctaa attacggact tcacttggaa gttttccctt gtatctttta taatagatag
     4141 aaaatttgct ggcagatgaa tatccaacag attctgctat ctcttttata ggtagttcag
     4201 tgtttaaaag aagagtttca gctacattca ttctttttct ttgagtgtac tctgtaatgc
     4261 tttgacaata tttttcctta aataaatttt ttaatttagt accactcatt ttagatattt
     4321 tttcaagcgt gccttgattt acattcgttg caaaatgatc atctaagaat cttgctacat
     4381 cttcaagtgc tttatcatca tcaatttcaa ttttatattt ttttctattt aagtatgtgt
     4441 caattactat acttatccat tcatttgcct ttgctttaaa gaaaaaatca gcggcaggag
     4501 cgtccatctt acaatttaat atttccattg ccactctttc taaggccttt gtaagtatta
     4561 tttgattcgg ttgaagcaag gttgaataaa aagattctgg attaatgtta atagatgcta
     4621 aatgtttttc tattagctct tttttaaaac caatggaaac agcaagataa caacaattct
     4681 cgtgtaataa aaaaacaaaa tcatctttta tattatcaaa atcaaaagta cataaagagt
     4741 ttgcggtaat agtttgatac ggattaaact tttctccgtt tgcactgaca atgtaacttg
     4801 aataaattga aacatagtct gacatactat aagtgctatt ttgaactact tcctctttga
     4861 tataaaaatc atgtatatcg ataatgaagc tgcctccttc ataaaaccaa tataggcctt
     4921 ctgcataagt tgaattctct tttttccaac aaaatgtatg acctgcactt gaatacttct
     4981 cattgttttt acattcatct actttcatat aatcttttac aaaatcataa tatgacataa
     5041 cacactatcc cttttagaca atattccaat tagccttatt aattcaaaac tattgtatta
     5101 gtaattataa cagatgtata atagaaaagc aatgatagat attatcaatt aagcgaattt
     5161 atatctaaaa gggatattaa agaaaggaga tatgcttatg aagatttaca aaaaactatt
     5221 tgcttatgtc caagataaga aatatcttgg ggttttggcc ataatttttt ctgctatatc
     5281 tgctgtactt acagtatatg gatattattt aatctacaaa tttctagata agttaataat
     5341 taattcaaac ttatccggtg cagagagtat agcattaaaa tctgttatta cactaacaag
     5401 tggagcgata ttttattttg tctcaggaat gttttcacat atcttgggat ttaggcttga
     5461 aacaaattta agaaaaaggg gaatcgatgg tctggaaaaa gcaagtttta ggttctttga
     5521 cttaaatcca tctggtcaaa taagaaagat catagatgat aatgcagcac aaactcatca
     5581 ggtggtagct cacatgattc cagatagctc tcaggcaata gtcacaccta tacttgtcct
     5641 tgtacttggc tttatagtaa gtataagagt tggcataatt ttgcttgctc ttactataat
     5701 tggtggctta attttagggg caatgatggg cgagcaagaa tttatgaaga tataccaaga
     5761 atccctatct aaactaagtg ctgaaactgt tgagtacgtg agaggaatgc aagttgtaaa
     5821 aatatttaaa gcaaatgtag agtcttttaa aagcttttat aaggcgataa aagattactc
     5881 aaagtatgct tatgattatt ccctatcttg taaaaggcct tatgttttgt atcaatggtt
     5941 attttttgga ctgattgcaa ttttaattat tcctatagtt tattttatga ctagcttagc
     6001 tagcgcagag gtgattttac ttgagcttat catgatttta tttttatcag gagttctctt
     6061 tgtttcattc atgagaatga tgtggtactc catgtatatt tctcaaggaa attatgcagt
     6121 agatacttta gaggcgcttt acgaagatat gcaaaaagac aaattagtgc atggtaatgt
     6181 caataatttt aaaaactata atatagaatt tgagaatgtt agctttgctt ataatgataa
     6241 agctatcatt gaaaatttat cctttaattt agaagaagga aagtcctacg cacttgtcgg
     6301 ttcatctgga tcaggcaaat caacagtagc aaaacttata tcaggttttt acaatgttaa
     6361 taaaggaagc ataaagatag gcgggatagc aataagtgaa tattctgacg aagccttaat
     6421 taaagccatt tcctttgttt ttcaagattc aaaattattc aagaagagca tttatgataa
     6481 tgtagcttta gctaataaag atgcgacgaa agatgacgtt atgagagcct taaaattagc
     6541 aggatgcgat ttaatattag acaaattccc agaaagagaa aatacaatca taggctcaaa
     6601 aggtgtttat ttatccggtg gagaaaaaca aagaattgca attgctagag caattttaaa
     6661 ggattccaaa attattatta tggatgaagc atcagcatct attgacccag ataacgagtt
     6721 tgaattgcaa aaagctttta aaaatcttat gaaggataaa acagttatca tgattgcaca
     6781 caggctatct acaattaaag accttgatga aattattgtc atggatagtg gaaaaattat
     6841 agaaagaggg tctgacaaag aattaatgtc aaaagataca aggtataaga gcctgcaaga
     6901 gatgtttaac agtgcgaatg aatggagggt ttcaaatgaa agagttttat aaaaaaagat
     6961 ttgctcttac agatggagga gcaagaaatt taagtaaagc aacactggct tcatttttcg
     7021 tttattgtat aaacatgctt cctgccatat tacttatgat ttttgctcag gaagttttgg
     7081 aaaatatggg caaaagcaat ggcttttata tagtattctc agttttgact ttgatagcaa
     7141 tgtatatttt gctttctatc gaatacgata aattatataa cacaacctat caagaaagtg
     7201 cagatttaag aataaggaca gcggagaatt tatcaaaatt acctctatct tacttttcta
     7261 aacatgacat ttccgacatt tcacaaacaa tcatggctga tattgaaggc atagagcatg
     7321 caatgagcca ctcaatacca aaggtgggcg gcatggtact gtttttccca ttaatatctg
     7381 taatgatgct agcgggcaat gtcaagatgg gtttagctgt aattattcca tctattttaa
     7441 gctttatatt tataccttta tctaaaaaat atcaggttaa tggacagaat agatattatg
     7501 atgtcttaag aaaaaactca gaaagctttc aagaaaatat cgaaatgcaa atggagatta
     7561 aagcatataa tttatcgaag gatattaaag atgacttata taaaaaaatg gaagatagtg
     7621 agagaataca cttaaaggcg gaagtaacta caattttaac tttgtctata tcttcaatat
     7681 ttagctttat atctcttgct gttgtgatat ttgtcggcgt aaatctaatt attaataaag
     7741 agataaattc tctctacctt ataggatatt tactagctgc tatgaagata aaagactctt
     7801 tagatgcatc taaagagggc ttgatggaaa tattttattt atcgcccaaa atagaaagat
     7861 taaaagaaat tcaaaatcaa gatttacaag aaggcgatga ctatagctta aaaaaatttg
     7921 atattgatct aaaagatgtt gagtttgcct acaataaaga cgcaaaagtt ttaaatggtg
     7981 taagttttaa agctaagcag ggagaggtca ctgctttggt aggtgcaagt ggctgcggta
     8041 aaacaactat cttgaaactt atatcaagac tttatgatta tgacaaggga caaatcttaa
     8101 tcgatggcaa agatataaaa gaaatatcaa cagaatctct ttttgataag gtctctatag
     8161 ttttccaaga tgtggttctc tttaatcaaa gcgttatgga aaatattaga atcggtaagc
     8221 aagatgcaag tgacgaagag gttaaaagag cagcaaaact tgcaaattgc acagatttta
     8281 tagaaaaaat ggataaaggt ttcgatacag ttattggtga aaacggagct gagctatcag
     8341 gaggagaaag acaaagatta tcaatagcca gagccttctt aaaagatgcg ccgatattga
     8401 tcttagatga gataacagca agccttgatg ttaacaacga gaaaaagatt caagagtctt
     8461 taaataattt agttaaagat aaaactgttg taatcatttc acatagaatg aaatccatag
     8521 aaaatgcaga caagatagta gttcttcaaa acggaagagt agaaagcgaa ggtaagcatg
     8581 aagagctttt acaaaaatca aaaatttaca aaaatttaat agaaaagaca aaaatggcag
     8641 aagaatttat ttattaggag gactacaatg gataataaaa aattaaaagt aaaagattta
     8701 gtaagtatcg gtgtttttgg cgtaatttat tttgccttca tgtttggagt tggtatgatg
     8761 ggcttgattc caatattgtt cttaatatac ccgacagtat tagccatagt tgcaggaact
     8821 gttgttatgt tatttatggc taaggttcaa aagccatggg cactatttat atttggtatg
     8881 atatcaccac ttgtgatgtt tgcagctggt catacctacg tagttgtggt tttatcactt
     8941 atagtaatga taatagcaga attaattaga aagattggta attataattc atttaaatac
     9001 aatatgcttt cttatgcaat cttcagcaca tggatatgta gctctttaat gcaaatgctt
     9061 ttagcaaaag aaaaatatat ggagtggtct ttgatgacga tgggaaaaga ttatgttgat
     9121 gtattagaaa agttaataac ttatcctcac atggctttaa tagccttagg tgctttctta
     9181 ggaggaattc ttggagcata tataggcaag gctctattga aaaaacactt ttcaaatgga
     9241 ttatattgtg tgggatactt tactccttgc ctaattttat ggtgttatct gaattaaacc
     9301 ctatagttaa gatgtttttg agtataccta ttgttattag aatgtttatt ttaccattta
     9361 tggcagcaag ctttatgata aagacctcgg atgtaggcgc aataatttca tcgatggata
     9421 agcttaagat ttcaaagaat gtatccatac ctattgcggt tatgtttaga ttcttcccat
     9481 cttttaagga ggagaagaaa aacatcaaaa tggctatgag agtaagaggg ataaatttta
     9541 aaaacccagt caaatatctt gaatatgttt ctgtgccact actcattata tcatctaata
     9601 tatcagatga cattgcaaaa gcggcagaaa caaaggcaat agaaaatcca attgccaaga
     9661 ccagatacat tcgcgtaaag atacagctaa ttgattttgt ttatgtttta gcggttgctg
     9721 gacttattgt gggaggctta atatggttga aataaaaaat ttaagtcttg attatggtga
     9781 agagcatata ttagatgata tatcactatc catagccgag ggagagtgcg tgctatttac
     9841 aggaaaaagt ggaagtggta agtcatcttt aataaattca atcaatggac tagctgtaag
     9901 gtatgataac gcaaagacaa agggcgaaat aattattgat ggtaagaata taaaaaattt
     9961 ggaactttat caaatctcaa tgcttgtttc aactgttttt caaaatccta agacatattt
    10021 ttttaatgtc aatacgacat tagaattatt attttatttg gaaaatatcg gtcttgcaag
    10081 agaagagatg gacaggcgtt tgaaggatat acttgagata ttcccgataa aaaatctttt
    10141 gaacagaaat atatttaatc tatccggcgg tgaaaaacaa attctttgca ttgcagcttc
    10201 ttatatagca ggtacaaaga ttatagttat ggatgagtct tcatcgaatt tagatattaa
    10261 aagcataagt gttttggcaa agatgctaaa gatattaaaa gagaaaggca taagcataat
    10321 tgttgcagag catagaattt attatttgat ggacatagtt gaccgtgtat ttttaataga
    10381 taaaggaaag cttaaaaaaa cttatactag aagtgaattt ttaaagctag ataaaaatga
    10441 attaaatgct ttaagtttaa gagataaaga attaagtaaa ttaaaagttc cttatttaaa
    10501 agaaggtgga gagtatcaga taaaaaatct tagctacaaa tttactgatg atgagtgttt
    10561 aagcttaaaa gatatttcgt tcaagcttgg gaaaatttat ggcataatag gatccaacgg
    10621 acgaggaaaa tcaacgcttt taagatgttt aataggtctt gagaaaaaat caaaagaaga
    10681 aatttatttt aagggagaga agctatctaa aaaagaaaga ctcaaaaact cttcacttgt
    10741 tatgcaagat gtaaatcatc aattattcac agatgaagta ttcaacgagc ttagattagg
    10801 agtaaagaat tttgatgaag aaaaggcgaa aatcatttta aaagatttag gcctggacga
    10861 atttattgaa aggcatccga tgagtttatc aggagggcaa aagcaaaggc ttgcaatagc
    10921 atctgttatg tgcaagactt ctccatttat cttttttgac gaaccttcaa gtggtataga
    10981 ttattccaat atgataaaaa tatctgaact gattaataag tataaaacca tggataaaat
    11041 aatttttatt gtttcccatg atatagaatt tttaaatgaa gtggcagatg aaatttttga
    11101 attgtaaaat agagtctgtt aaacatgtct atagaactgg taaattgtct aaaaatttaa
    11161 aagatactaa cacatattga tatttattag aaaattaact tcaggatttt tacagtattt
    11221 ataaacttta taatatgtat ttatagtatt aataatttcc atatctttag ggaaattatt
    11281 tatttactta ccataattaa agagatgaga gataaatatc ggcagtcttc ggactgcctt
    11341 tattttttta cttaatttaa tgcagtaaat catacgattg aaaggaatct gcgttatatg
    11401 ctataattag gtagttaatc tatacattta gaaaggtaaa atatagaatt ggagaatgat
    11461 atgggatttt catataataa attgtaacca cccaataacg aagtctattg aaaaatctcc
    11521 agattagtga actcacggat agttcctaat ctggagattt cttatttgca cttttcttgt
    11581 acaactttag tcc
//
LOCUS       DAVBCW010000058        11507 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVBCW010000058 DAVBCW010000000
VERSION     DAVBCW010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11507
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.058"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            131..385
                     /gene="blpM"
                     /locus_tag="VQ514_001336"
     CDS             131..385
                     /gene="blpM"
                     /locus_tag="VQ514_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpM"
                     /protein_id="HET2500088.1"
                     /translation="MDTKIMEQFHEMDITMLSSIEGGKNNWQTNVLEGGGAAFGGWGL
                     GTAICAASGVGAPFMGACGYIGAKFGVDLWAGVTGATGGF"
     gene            401..604
                     /gene="blpN"
                     /locus_tag="VQ514_001337"
     CDS             401..604
                     /gene="blpN"
                     /locus_tag="VQ514_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="HET2500089.1"
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGRLLGP
                     WGAAIGGIGGAVVCGYLAYTATS"
     gene            848..997
                     /gene="blpO"
                     /locus_tag="VQ514_001338"
     CDS             848..997
                     /gene="blpO"
                     /locus_tag="VQ514_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HET2500090.1"
                     /translation="MDTKMMSQFAVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
     gene            1101..1220
                     /locus_tag="VQ514_001339"
     CDS             1101..1220
                     /locus_tag="VQ514_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HET2500091.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     gene            2006..2404
                     /locus_tag="VQ514_001340"
     CDS             2006..2404
                     /locus_tag="VQ514_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HET2500092.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEVGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKKQTVFLILQQ"
     gene            2442..3131
                     /locus_tag="VQ514_001341"
     CDS             2442..3131
                     /locus_tag="VQ514_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2500093.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQLTLIVQNYWQFSSQIGNFV
                     WIQNILSLLFIGVMIVVLVKTGHGYLFRIPRKKWLWYSILTVLVVVFQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYIALFFLVPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFVFATVGIIFAGLTRYTKSIYPSYAVHVINNIVATFP
                     FLLTFLHRVLG"
     gene            3173..3421
                     /locus_tag="VQ514_001342"
     CDS             3173..3421
                     /locus_tag="VQ514_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500094.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLKWFVLERLELKDDA"
     gene            3451..4062
                     /locus_tag="VQ514_001343"
     CDS             3451..4062
                     /locus_tag="VQ514_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2500095.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGGFFQNESALILVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMFYNSWPLLYYL"
     gene            4223..5017
                     /locus_tag="VQ514_001344"
     CDS             4223..5017
                     /locus_tag="VQ514_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HET2500096.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            5014..5649
                     /gene="trmB"
                     /locus_tag="VQ514_001345"
     CDS             5014..5649
                     /gene="trmB"
                     /locus_tag="VQ514_001345"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HET2500097.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            5775..6254
                     /gene="rimP"
                     /locus_tag="VQ514_001346"
     CDS             5775..6254
                     /gene="rimP"
                     /locus_tag="VQ514_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HET2500098.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEIISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            6298..7434
                     /gene="nusA"
                     /locus_tag="VQ514_001347"
     CDS             6298..7434
                     /gene="nusA"
                     /locus_tag="VQ514_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HET2500099.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            7456..7749
                     /locus_tag="VQ514_001348"
     CDS             7456..7749
                     /locus_tag="VQ514_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HET2500100.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            7742..8041
                     /locus_tag="VQ514_001349"
     CDS             7742..8041
                     /locus_tag="VQ514_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HET2500101.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            8058..10850
                     /gene="infB"
                     /locus_tag="VQ514_001350"
     CDS             8058..10850
                     /gene="infB"
                     /locus_tag="VQ514_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HET2500102.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     VKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            11101..11451
                     /gene="rbfA"
                     /locus_tag="VQ514_001351"
     CDS             11101..11451
                     /gene="rbfA"
                     /locus_tag="VQ514_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HET2500103.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
BASE COUNT         3514 a         2015 c         2620 g         3358 t
ORIGIN      
        1 taatttttgc aattcaagac gtttcgatga ctattcaaga tctggatgaa aaattttaaa
       61 aaacgatgat atactaaact tgtcaaagtt acaacaagac aaaaattaaa aataaaaaag
      121 agtatttgtc atggatacaa aaattatgga acaatttcat gaaatggata taactatgtt
      181 atctagtatt gaaggaggca agaataattg gcaaactaat gtcttggaag gtggtggtgc
      241 tgcttttggt ggctgggggc tagggacagc tatttgtgct gcgagtggtg ttggagcacc
      301 atttatggga gcatgtggat acataggagc taaatttggt gtggatcttt gggcaggagt
      361 aacaggggca acgggtggat tttaataaaa ggagacttgt atgaatactt attgtaatat
      421 aaatgaaaca atgctgagtg aggtttatgg aggtaattcc ggaggagcag ctgtagttgc
      481 tgctttaggt tgtgcagcgg gtggagtgaa atatgggaga cttctaggac catggggcgc
      541 tgcaatagga ggaattggag gagcagtggt ttgtggatat ttagcctata ccgctacatc
      601 ataaaaatga aagctaaatt ttatgaaggc tatattcttt attattctct tcgcttttca
      661 aacctatctt atttatctat ccataagtat ctccgataaa aaacaaaaga ctattgaatt
      721 aacatcattg aattgttttg tcatattatt tctaatctat gataaattaa tttttctctt
      781 tattgcctat gtttttttga taatttttat attaaacttg ttccgtaact gaaaagtgat
      841 atactatatg gatacaaaaa tgatgtcaca atttgcagtt atggataatg aaatgcttgc
      901 ttgcgttgaa ggtggagata ttgattgggg aagaaaaatt agttgtgcag caggggttgc
      961 atatggcgca attgatgggt gtgcaacaac ggtttgacat ttctattggg accatttgct
     1021 ataggaatag gtgtaactgg tgctgcaggt ggagctatac ttggaggtgt agcctatgca
     1081 gcaatatgtt ggtggtaatt atggatttta aaagttttat tattggttta gtagttggta
     1141 tatttggtcc ttatatggat gacttaatta gaaaaatatt ttcaaaatct tccaaaaagg
     1201 atacggatag tactctctaa aaaataaatt tctcccctgt atttgctagc ttaatttaca
     1261 ggggagtttc tttaatattg tttcgcctgt caaaatgata tgtgatagca ggatgaaaag
     1321 tctacaaaca agttggtatc aatacaatat aaagaggaaa gtatgttttt aatgcttact
     1381 ttttaaattt tttaacaatt caagatgttt cgatgacaat tcaagatttg gatgaaaaat
     1441 tttaaaaaac aatgatatac taaacttgtc aaagttgcaa cgggtgctgc tacattatgt
     1501 taaaaatcgc tcttgattga taagctggat ttgttaggaa aggagtaaat aattatggat
     1561 aagaaaaaga tagtttcgac tataatatgt atagtatttc ttgtagtgtc agtagataat
     1621 ttttttagag acttaacccc gttactgttc attttaaata ttattggcct gtcttgcttt
     1681 tcagtgctaa catacattaa cataaaagag atactcttaa atataagtaa atgatgattt
     1741 ggttagtcct agaaccaatc aacttatgtt tatcggcttg gcagatttta tgtttgtcat
     1801 ttgtttgtat agaggtattt cagaaacaga attctatcaa caactaatcg cttatatagg
     1861 tgtattctct gcttgtttat cacgcttctg ctcatgtggg gcttgaagta ttataaaaag
     1921 tagctttcta tccttttctg acaggatatt tgagtagaag gctctattaa attgttagtg
     1981 aaataaatga atggaggtat ttaatatgaa atacagatta ttttttgtta ttttcttgag
     2041 tagtgtgttg gatattcttt tagggacatt tttacaaatc tctatcgtat ctattgggtg
     2101 gcttgttctt tacagtggtt tgtttgaagt aggagttttc cttcttgcta ataaaggggt
     2161 ggcggtaaaa atcaaggaag tagatattcg aaatcgcttt aaatttattt ttggaaaaac
     2221 cttatggttt caaattcttt tgctcatctt tttgataatc aaactttatc ttggtttgga
     2281 tgcgaggttg attttattct atggacatat tttcattgtc tttaatgcct taatgtatct
     2341 attatctagt agtcaggtta gccttaaaaa aaaacaaact gtcttcttaa tcttacagca
     2401 gtagagtctg acaaggtgag gtcatagtag ggaggatttc tatgaaaaag tatcaacttc
     2461 tattcaaaat aagtgcagtc ttctcttact tatttttcgt atttggtctt tctcagctga
     2521 cgcttatcgt ccaaaattat tggcaatttt cttcccagat tggtaatttc gtctggattc
     2581 aaaatatctt gagtttactt tttattggag tcatgattgt ggttcttgtt aagacaggcc
     2641 atggttatct cttccgcatt ccaagaaaaa aatggctttg gtattcgatt ttgacagtat
     2701 tagtggtagt gttccagatc tcttttaacg ttcagacagc taaacatgtt cagtcaactg
     2761 cggaaggttg ggctgtattg attggttata gtgggactaa ctttgcagag ctaggtattt
     2821 atatagccct gttctttctg gttccactga tggaagaatt gatttataga ggattactgc
     2881 aacatgcttt ctttaagcat tcgcgatttg gtcttgattt gcttcttcct tctattttat
     2941 ttgctctccc tcatttttca agcctgccta gtctgttaga tatcttcgtc tttgcaacag
     3001 ttggaatcat ctttgctggt ttgacccgct ataccaagag catttatcca tcctatgcgg
     3061 tgcatgtgat caataatatt gtagcgacct tcccgttttt gctcactttt ctacataggg
     3121 tcttggggta aaaaaacaaa aggcttgctt ttcagccata gaggaggtca tcatgtataa
     3181 acacttattt ttcctagatt ccaaaacttt agatcggttg acaccctata ttctagtctt
     3241 ggcttctgac accattgctt ttaatgtttt tgtgctaacc tttgtatctg cggtggtttt
     3301 taatttccta aattccatgc tagctttaat ggctatattc ataggggctg gctatgtggt
     3361 cggattttgg ttgctaaaat ggtttgtttt ggaaaggtta gagctaaagg atgacgcgta
     3421 ggaagtctgt ttggtgagga ggatagtttt atggagtttt ttgataaatt tcatgccttg
     3481 tgttttggat ttttagtact aataattgtc attacagttc cttatacgat taaccatggg
     3541 ggtttttttc aaaatgaatc tgcattgatt cttgtaagtc ttcttgtaac ctcgctgagt
     3601 gttgcttatg ctagaaagtt tgaaatgatt tcttttggga tgttaagcaa gaaacaactt
     3661 ttgcttttca ttgcaatctt tcttctaagt gtacttgaga cgctggttta tattcatttc
     3721 ttcgctgttt cttctggctc aggggtccaa cacttggcgg aagtcagcag aggaatttcc
     3781 ctgtctttga ttttgactac ctcagttttt ggccccatcc aggaggaact cattttcaga
     3841 ggacttcttc aaggtgcggt ttttgacaat tcttggttag ggcttgtgct aacttcctct
     3901 ctcttttctt tcatgcatgg accttctaat gtcccttcgt ttatttttta tctacttggg
     3961 ggtttgttgc tgggctttgc ttataaaaag agtcaaaacc tatgggtttc tactctagta
     4021 cacatgtttt acaacagttg gccactccta tattatttat aaaaattatg aaaaggtaag
     4081 cagaagacgg tgcttacctt tttcaagcta taatgatacc caagccaatc cagaggcttt
     4141 tctttgagaa tcgggttaga gtggttgacg aataggccaa aaactagtag aatagtaagg
     4201 aaactttata cggaggaaag aaatggattt gggtgataat gagctaacac tgactcccat
     4261 acctgggaaa agtggcaagg cttatatggg tagctatcct gacgggaagc gcatctttgt
     4321 aaaaatgaac acctctccaa tcctacctgg tctagctaga gaacaaattg ctccacaatt
     4381 attatggagt cgtcgtttgg cagatgggcg tgatatgtgt gctcaagaat ggttgacagg
     4441 caagatattg accccctatg atatgaatcg taagcaaatc gtcaatattt taacccgtct
     4501 tcatcgctca cgtccgttga tgacacaatt gagtcgtttg ggctatgcca tggaaacacc
     4561 tgtagattta ctacagtctt ggcaggaaac ggctccagat gctttgcgta aaaatcattt
     4621 tatcagtgaa gtgatggctg atttacgtca gactattcca ggatttagag aggaccatgc
     4681 gaccattgtc catggagatg tacgacatag taattggatt gagacagata gtggcttgat
     4741 ttatttagta gattgggatt cggttcgctt gaccgatcgc atgtttgatg tggcccatat
     4801 gctctgccat tatatttcag aacatcagtg gaaggaatgg ttgacctact acggttacaa
     4861 gtacaatcaa acggtattaa gtaaattgta ttggtatggt caattgtctt atttgagtca
     4921 gatttccaag tattatatga accaagattt agaaaatgtc aatcgggaga ttcatggttt
     4981 gcgtcatttc cgagacaagt atggaaagag aagatgagag ttagaaatcg taaaggggca
     5041 acagaattac tagaggcaaa tccccagtat gtggtcctca atcccttgga agccaaggca
     5101 aaatggcggg acttgtttgg caatgataat cccattcatg tggaagttgg aagtggaaag
     5161 ggtgcctttg tttcaggtat ggccaagcaa aaccctgaca tcaactatat cgggattgat
     5221 attcaaaagt ctgttttgag ctacgctttg gacaaggtgc ttgaagttgg agtgcctaac
     5281 atcaagctct tgtgggtaga tggttctgac ttaactgact attttgaaga cggtgagatt
     5341 gatcgcttgt atctgaactt ttcagatcca tggccgaaaa aacgccatga aaagcgtcgt
     5401 ttgacctaca agaccttctt ggataccttc aaacgtatct tgcctgaaaa tggagaaatt
     5461 catttcaaga cggataaccg tggcttgttt gagtacagtt tagtgagctt ttctcaatat
     5521 ggcatgaaac tcaatggtgt ctggttagat ttgcatgcca gtgattttga aggcaatgtc
     5581 atgacagaat acgagcaaaa attctcaaac aaggggcaag ttatctaccg agttgaggca
     5641 gaattttaag agatagccta aaattaggct gtacaagtgc ttttgcttta cataagttgg
     5701 caaacgtgct atactgatag taagaatatg aaaagtgagg cggggaaata tcttcgcctc
     5761 ttgcttatga ggaggtggac gcaatcgcaa caatcgtaga attagtcaga gaagttgtag
     5821 aacctgtcat agaagctcct tttgaactcg tggatatcga gtatggaaag attggcagtg
     5881 acatgattct cagtattttt gtagataaac ccgaaggaat taccttgaac gacacggcag
     5941 acttgacaga aattatcagt cctgtcctag acaccatcaa gccagatccc ttcccagaac
     6001 aatatttcct agaaattacc agtccaggtt tggaacgccc tttgaaaacc aaggatgccg
     6061 tcgctggagc ggttggaaaa tacatccatg tcgggctcta ccaagccatc gataagcaaa
     6121 aggtctttga aggaaccttg ttggccttcg aagaggacga gttgactatg gaatatatgg
     6181 acaagacgcg taagaaaacc gtccaaattc catacagttt agtatcaaaa gcacgtttag
     6241 cagttaaatt atagaaaaag aaaggatagc ttttgaggat tcaaaagtga agaaaacatg
     6301 agtaaagaaa tgctagaggc cttccgcatt ttggaagaag acaagggaat caaaaaagaa
     6361 gatatcatcg acgcagtagt agagtcgctt cgttccgctt atcgcagacg ctatggtcag
     6421 tcagacagcg tagctattga cttcaacgaa aaaacaggtg actttacagt ttatactgtc
     6481 cgtgaagttg ttgatgaagt atttgatagc cgtttggaaa tcagcttgaa agatgctctt
     6541 gccattaatt cagcttatga acttggagac aaaatcaagt ttgaagaagc accagctgag
     6601 tttggtcgtg tagcagccca gtctgccaaa caaaccatca tggaaaaaat gcgcaagcaa
     6661 acacgtgcca tcacttacaa tacttacaaa gaacatgagc aagaaatcat gtctggtaca
     6721 gtagaacgct ttgacaaccg ctttatctat gtcaaccttg gtagcatcga agcccaattg
     6781 tcaaaacaag accaaattcc tggagaagtt tttgcttctc atgatcgtat cgaagtttat
     6841 gtttacaagg ttgaagacaa ccctcgtggt gtgaacgtct ttgttagccg tagtcatcca
     6901 gaaatgatca aacgtttaat ggagcaagaa attccagaag tttatgatgg aactgttgaa
     6961 atcatgagcg tggctcgtga agcaggtgac cgtacgaagg ttgctgttcg tagccacaat
     7021 ccaaacgtgg atgctatcgg tacaatcgtt ggacgtggtg gtgctaatat caagaagatt
     7081 actagcaaat tccacccagc tcgttacgat gctaaaaatg accgcatggt accaatcgaa
     7141 gaaaatatcg atgttatcga gtgggtagca gatccagctg aatttatcta caatgccatc
     7201 gctcctgctg aggttgacca agttatcttt gatgaaaacg acagcaaacg tgccttggtg
     7261 gttgttccag ataacaagct ttctcttgcc attggtcgtc gtggacaaaa cgtgcgcttg
     7321 gcggctcact tgactggtta ccgtatcgat atcaagtctg ctagcgaatt tgaagccatg
     7381 gaagacgctg cttcagtaga gttggaagta gaaaacgata ctgtagaaga ataaaagctg
     7441 ctagaggagg gaaagatgaa aacaagaaaa atccctttgc gcaagtctgt tgtgtctaac
     7501 gaagtcattg ataagcgtga tttgctccgc attgtcaaga acaaggaagg acaagtcttt
     7561 attgatccta cgggcaaggc caatggccgc ggcgcttata tcaaactaga caatgcagaa
     7621 gccctagagg cgaaaaagaa gaaggtcttt aaccgcagct ttagcatgga agtggaagaa
     7681 agcttttatg acgagttgat cgcttatgtg gatcacaaag tgaaaagaag agagttggga
     7741 cttgaataag caaaagataa gtaatctctt ggggcttgct cagcgagcag ggcgcatcat
     7801 atcgggtgaa gaattggtgg tcaaggccat tcaagacggc aaggccaagt tggtctttct
     7861 agctcatgat gctggaccca atctgaccaa gaagattcaa gataaaagtc attattatca
     7921 agtagaaatt gtaaccgtgt tttcaacact ggaattaagc atagcagtcg ggaaatcgag
     7981 aaaggttttg gctgtaacag atgctggatt tacaaagaaa atgaggtctc ttatggaata
     8041 gaagaggagg acatgatttg tctaagaaaa gattgtacga aatcgcaaaa gaacttggaa
     8101 aagaaagtaa agaagttgta gcgcgtgcaa aagagttggg cttggatgtg aaaagccact
     8161 catcaagtgt ggaagaagct gtcgctgcaa aaattgctgc cagctttaag cctgcagctg
     8221 ctccgaaagt agaagcaaaa cctgcagcac caaaagtaag tgcagaaaag aaagccgaaa
     8281 aatctgagcc agctaaacca gctgtagcta aggaagaggc aaaaccggct gagccagttg
     8341 ctccgaaaac agaaaaagta gcagtgaaac cgcaaagtcg taatttcaag gctgagcgtg
     8401 aagcccgtgc taaagagcag gcagaacgac gcaagcaaaa taagggcaat aaccgtgacc
     8461 aacaacaaaa cggaaaccgt cagaaaaacg acggccgtaa tggtggaaaa caaggtcaaa
     8521 gcaaccgcga caatcgtcgc tttaatgacc aagctaagaa gcagcaaggt cagcaaaaac
     8581 gtagaaatga gcgccgtcag caagaggata aacgttcaaa tcaagcggct ccacgtattg
     8641 actttaaagc ccgtgcagca gccctaaaag cagagcaaaa tgcagagtac gctcgttcaa
     8701 gtgaggaacg cttcaagcag tatcaggctg ctaaagaagc cttggctcaa gctaacaaac
     8761 gcaaggaacc agaggaaatc tttgaagaag cggctaagtt agctgaacaa gcacagcaag
     8821 ttcaagcagt ggttgaagtc gtccctgaga aaaaagaacc tgcagtggat acacgtcgta
     8881 aaaaacaagc tcgaccagac aaaaatcgtg acgattatga tcatgaagaa gatggtccta
     8941 gaaaacaaca aaagaatcga agtagtcaaa atcaagtgag aaatcaaaag aatagtaact
     9001 ggaataacaa caaaaagaac aaaaaaggca ataacaagaa caaccgtaat cagactccaa
     9061 aacctgttac ggagcgtaaa ttccatgaat tgccaacaga atttgaatat acagatggta
     9121 tgaccgttgc ggaaatcgca aaacgtatca aacgtgaacc agctgaaatc gttaagaaac
     9181 ttttcatgat gggtgtcatg gccacacaaa accaatcctt ggatggggaa acaattgaac
     9241 tcctcatggt ggattacggt atcgaagcca aacaaaaggt tgaagtggat aatgctgaca
     9301 tcgaacgttt ctttgtcgaa gatggttatc tcaatgaaga tgaattggtt gagcgtccac
     9361 cagttgttac tatcatggga cacgttgacc acggtaaaac aacccttttg gatactcttc
     9421 gtaactcacg tgttgcgaca ggtgaagcag gtggtattac tcagcatatc ggtgcctacc
     9481 aaatcgtgga aaatggtaag aagattacct tccttgatac accaggacac gcggccttta
     9541 catcaatgcg tgcgcgtggt gcttctgtta ccgatattac gatcttggtc gtagcggcag
     9601 atgacggggt tatgcctcag actattgaag ccatcaacca ctcaaaagca gctaacgttc
     9661 caatcatcgt agccattaac aagattgata aaccaggtgc taacccagaa cgcgttatcg
     9721 gtgaattggc agagcatggt gtgatgtcaa ccgcttgggg tggagattct gaatttgttg
     9781 aaatctcagc taaattcaac caaaatatcg aagaattgtt ggaaacagtc cttcttgtag
     9841 ctgaaatcca agaactcaag gcagacccaa cagtgcgtgc aatcggtacg gttatcgaag
     9901 cgcgcttgga taaaggaaaa ggtgcggtcg caacccttct tgtacaacaa ggtaccttga
     9961 atgttcaaga cccaatcgtt gtcggaaata ccttcggtcg tgtccgtgct atgaccaacg
    10021 accttggtcg tcgtgttaaa gttgctggac catcaacacc agtctctatc acaggtttga
    10081 acgaagcacc gatggcgggt gaccactttg ccgtttacga ggatgaaaaa tctgcgcgtg
    10141 cagcaggtga agagcgtgcc aaacgtgccc tcatgaaaca acgtcaagct acccaacgtg
    10201 ttagccttga aaacctcttt gataccctta aagctgggga actcaaatct gttaatgtta
    10261 tcatcaaggc tgatgtacaa ggttctgttg aagccctttc tgcctcactt caaaagattg
    10321 acgtggaagg tgtcaaagtg actatcgtcc actcagcggt cggtgctatc aacgaatcag
    10381 acgtgaccct tgccgaagct tcaaatgcct ttatcgttgg tttcaacgta cgccctacac
    10441 cacaagctcg tcaacaagca gaagctgacg atgtggaaat ccgtcttcac agcattatct
    10501 acaaggttat cgaagagatg gaagaagcta tgaaagggat gcttgatcca gaatttgaag
    10561 aaaaagttat tggtgaagcg gttatccgtg aaaccttcaa ggtgtctaaa gtgggaacta
    10621 tcggtggatt tatggttatc aacggtaagg ttgcccgtga ctctaaagtc cgtgttatcc
    10681 gtgatggtgt cgttatctat gatggtgaac tcgcaagctt gaaacactat aaagacgacg
    10741 ttaaggaagt tacaaacggc cgtgaaggtg gattgatgat cgacggctac aatgatatta
    10801 agatggatga tgtgattgag gcgtatgtca tggaagaaat caagagataa gattttttgc
    10861 tcctttctta ggtggtgagg gacgcaagca aaccgatggt ttcattgctt atttttgagc
    10921 ctagggtctc aaaaatcccc tgtgatggga ctgataaatc agttccatca ctttcaccac
    10981 ggcgaaagaa gcagatgact tcaaattgaa cttcgtttca atttaaactg aaaatcaaga
    11041 agtttaaaat agctaggtct gctggcctag cttttggttc aaagtagaga aaggaatatc
    11101 atggcaaatc atttccgtac agatcgtgtg ggcatggaaa tcaagcgtga agtcaatgag
    11161 attttgcaaa agaaagtccg tgatccacgt gtccaaggtg tgaccatcac agatgttcag
    11221 atgctgggtg acttgtctgt tgccaaggtt tattacacca ttttgagtaa ccttgcttcg
    11281 gataaccaaa aagcccaaat cgggcttgaa aaagcaactg gtaccatcaa acgtgaactt
    11341 ggtcgcaatt tgaaattgta caaaatccca gatttgacct tcgtcaaaga cgagtccatc
    11401 gagtatggaa acaagattga cgagatgcta cgcaatctgg ataagaacta aagaagaggg
    11461 gttgcccctc tttttcggct ctttgtcaac tgtagtgggt tgaagaa
//
LOCUS       DAVBCW010000059        11392 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVBCW010000059 DAVBCW010000000
VERSION     DAVBCW010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11392
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.059"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(39..2036)
                     /gene="metG"
                     /locus_tag="VQ514_001352"
     CDS             complement(39..2036)
                     /gene="metG"
                     /locus_tag="VQ514_001352"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET2500104.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVAN
                     LKPRKMMKKYVSQGMILSAEYDGKLTLLTVDPAVPNGSVIG"
     gene            complement(2160..3419)
                     /locus_tag="VQ514_001353"
     CDS             complement(2160..3419)
                     /locus_tag="VQ514_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2500105.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKKSKIYGFGGLP
                     ITTNISLAANFNIDEIASIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(3421..4122)
                     /locus_tag="VQ514_001354"
     CDS             complement(3421..4122)
                     /locus_tag="VQ514_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2500106.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(4106..5305)
                     /locus_tag="VQ514_001355"
     CDS             complement(4106..5305)
                     /locus_tag="VQ514_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET2500107.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTAVKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAQAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILIPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            5591..6937
                     /gene="gor"
                     /locus_tag="VQ514_001356"
     CDS             5591..6937
                     /gene="gor"
                     /locus_tag="VQ514_001356"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HET2500108.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESIAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
     gene            7134..7670
                     /locus_tag="VQ514_001357"
     CDS             7134..7670
                     /locus_tag="VQ514_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HET2500109.1"
                     /translation="MKKAHVYAIPAIGAALIAALAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALAVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            7721..7807
                     /locus_tag="VQ514_001358"
                     /pseudo
     CDS             7721..7807
                     /locus_tag="VQ514_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(7876..8091)
                     /locus_tag="VQ514_001359"
     CDS             complement(7876..8091)
                     /locus_tag="VQ514_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HET2500110.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            8189..8509
                     /locus_tag="VQ514_001360"
     CDS             8189..8509
                     /locus_tag="VQ514_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500111.1"
                     /translation="MRHKFQQVLNKIHDFLNGYDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFAKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            complement(8540..8878)
                     /locus_tag="VQ514_001361"
     CDS             complement(8540..8878)
                     /locus_tag="VQ514_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500112.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(8890..9609)
                     /gene="trmD"
                     /locus_tag="VQ514_001362"
     CDS             complement(8890..9609)
                     /gene="trmD"
                     /locus_tag="VQ514_001362"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HET2500113.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            complement(9599..10117)
                     /gene="rimM"
                     /locus_tag="VQ514_001363"
     CDS             complement(9599..10117)
                     /gene="rimM"
                     /locus_tag="VQ514_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HET2500114.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKRYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            complement(10148..10936)
                     /locus_tag="VQ514_001364"
     CDS             complement(10148..10936)
                     /locus_tag="VQ514_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET2500115.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADAVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKRQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFEDVSIEAHIPVFVFCQP
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELSE"
BASE COUNT         3274 a         2506 c         2076 g         3536 t
ORIGIN      
        1 aaaaaaacag aactagtctg aactagtcct gtctactttt acccaatcac acttccattt
       61 ggtacagctg gatcaactgt gagaagggtt aatttgccat catattcagc tgatagaatc
      121 ataccctggc tgacatattt tttcatcatt ttacgtggtt tgaggttagc aacgatttga
      181 actttcttgc cgaccaattc ttgttcattt ggatagtatt ttgcaattcc tgaaagaatc
      241 tgacgatctt ctccatcacc agcatccaag cggaattgaa gcaacttatc tgaaccttct
      301 actttagaca cttctttgac ttctgcgaca cggatttcaa ccttgtcaaa gtcttcaaac
      361 ttgatttcat ccttgtttag tttgagctca acttcgtccg gattccattc tttttcgact
      421 gctggtttat tgccttccat ttgttccttg atataggcga tttcttcttc catatttaga
      481 cgtggaaaga taggtgttcc tttggcaact acagtcacat ctgctgggaa gtcagccaaa
      541 ctcaagtttt caagactaga aacttcttcc aaaccaagtt gagtcaaaac tgcacgacta
      601 gtttccatca taaatggttc aatcaagtga gcaactacac gaatgctggc tgccaagtgg
      661 ctcatgacac ttgccaattg gtcacgaaga gcttcatcct tggccaagac ccatggtgcg
      721 gtctcatcga tgtatttatt ggtacgagag atcagagtcc agactgcttc aagcgcacgt
      781 ggatagtcaa ctgcttccat gtgtgtatgg aagtctgcga ttgattgttc tgcaacctca
      841 gcaagaacat gatcaaattc agtcacacct tctacatagg cagggatttg tccatcaaag
      901 tacttattaa tcatggaaac cgtacggtta aggaggttcc caaggtcatt agccaattca
      961 tagttgatac ggccgacata gtcttcagga gtaaaggttc cgtctgaacc aactggaagg
     1021 ttacgcatga ggtagtaacg aagtggatct agtccataac gctctaccaa catttcaggg
     1081 taaacgacat tcccttttga cttagacatt tttccgtctt tcatgacaaa ccaaccatgg
     1141 gcaatcaaac gatcaggtaa tttaacatcc aacatcataa gaaggattgg ccagtagata
     1201 gagtggaagc gaaggatatc ttttcctacc atatggaaga ctgttccatt ccagaacttg
     1261 tcaaagttac catgttcgtc ttgagcgtag ccaagagctg tcgcatagtt aagaagggca
     1321 tcaatccaaa cgtagacaac gtgttttgga tttgatggga caggcactcc ccatgtaaag
     1381 gttgtacgag ataccgccaa atcttccaag cctggctcga tgaagttgcg tagcatttca
     1441 ttaaggcgac catctggcgt gataaattca ggatgagctt tgaaaaattc gaccaaacgg
     1501 tcttggtatt tgctaaggcg aaggaagtat gattcttcag aaacccattc aacctcatga
     1561 cctgatggag caataccacc agtcacattt ccagcttcat cacggaaaac ttctgccagc
     1621 tggctttctg taaagaattc ttcgtctgat actgaatacc aaccagagta ttcacccaag
     1681 tagatatcat cttgagcaag taagcgttca aagacctgtg cgacaacttt ttcatggtag
     1741 tcatcggttg tacggataaa tttatcgtat gagatatcta gtaattgcca gagttcttta
     1801 actccaaccg ccattccatc aacataggct tgaggtgtaa taccagcttc ttccgctttc
     1861 tgctggattt tctgaccatg ttcatcaaga cctgtcagat aaaatacatc gtagcccatc
     1921 aggcgtttgt aacgtgctag gacatcacat gcgatagttg tgtaggcaga accgatatga
     1981 agtttcccag atggatagta aatcggcgtt gtaatataaa aatttttttc agacataatt
     2041 tttcctttcc aggcaaatga aacctgtttt tctaacactt cattatatca catttttaat
     2101 gaatttcgat agggaaatcc atacaaaaac aagatagacg agtgtccatc ttgttgatct
     2161 cattcataac gaagggcttc aattggatca agtttcgatg ccttgttggc tggcaagact
     2221 ccaaaaatca taccaacact agccgaaact gcaagactaa atagggcaac tgggattgat
     2281 actccaactt ctataccttc tattaaacct tgcagtaaca aacctgctaa ggcagttaaa
     2341 ccacttgcaa ttgtcaagcc aattaagcca cctaacaagg tcaaaatcat ggattcaatc
     2401 aaaaactgaa ttaaaatatt ggcacgtgtt gcacccaaag ctttacgaag accaatctca
     2461 cgagtgcgct ctgtcaccga aaccagcatg atgttcatga caccagttcc tccaacaaag
     2521 agagaaatcc ctgcgatgga actaataatc gtcgtcataa aactaaacga ttgttgaatt
     2581 tctgcaaata caacggactc atctgccacc tggtattctc cctgttgtaa gcctgcaagc
     2641 tctgtcattt ttcgtgccag ttctggaccc agagttgggg ttaaactggt atcattcact
     2701 cgaaagacaa tactagctat ttcatctata ttaaaattcg cagcaaggga gatattggta
     2761 gtaataggca agccaccaaa cccatatatt tttgactttt tagcctccgg actagtataa
     2821 accccaatga cccggtaact aaatccattg acttctacaa ccttgttaat agcctcttga
     2881 ggagattcaa ataaactaat ggacaattcc tcatctagca aaataacact tgcaaactct
     2941 ttgaaatctt gctctctcag actacgacct gcaataattt cattcttaac agcgtccatg
     3001 taagttctgt ttccacctgt caaattagca ttctcaacct ttttatcttg ataggtcaag
     3061 atggcattcg ttgaattggt tacatagtaa ctatccactc ccttcagttt agctgcctct
     3121 tgaacccagg attcttgcgg ttttggcggt tcaacaggaa cttcctcttc ctttccagaa
     3181 accgtaaaag ctgattgttt ctgagtaaaa gacccatctt tacttttttt aggagagaaa
     3241 aagacgctaa tatttttctg agatttagtc atatctttat tgacttgacg agatagggaa
     3301 tcacccaaag ccataatcac aacaactgat gaaacaccga taataatccc aatcatagta
     3361 agcaaagaac gcatcttgtg agccatgata gatgaaaagg caaatttcag attctgcatc
     3421 ttagttttcc tcctttccta actgagcact gtcagacgaa atgaccccat cccgaatgac
     3481 aatctgacgt ttggcatagg cagcaatctc aggctcatgc gttaccatga taatggtttt
     3541 tccttcttta ttcaaatcaa ccaataattg cataatttgg ttacctgttt tggtatccaa
     3601 ggctcctgtc ggttcatccg ctaggataat agaaggattg tttaccaagg cacgcgcaat
     3661 ggctacacgt tgcttttgac caccagataa ttctgaaggt aaatggtgac tacgttctgt
     3721 caattcaacc ttgtctaaat attcctcagc caacttgcga cgttttgaag acgaaactcc
     3781 tgcgtaaatc aagggcaatt ctacattttg cagagcattg agcttcgata gaagaaagaa
     3841 ctgctgaaag acaaaaccga tttgttggtt acggacctta gctagttgtt tttcaccaag
     3901 cccagccact tcttgacctt caagataata ttctccactg gttggtgtat ccaacatgcc
     3961 aatcgtattc atcagagtgg acttaccaga cccagatggt cccatgatgg ctacaaattc
     4021 accctcattc acttctagat tgatattttt gagaacctgc agttcttggt caccattacg
     4081 gtaacttctg aagatatttt ttagactaat tagttgcttc atcagccttc acctcttttc
     4141 cttcttccaa ggaagatgtt ggattactga tgaccttagc accgttcgtt aaaccagaag
     4201 tgatttcttg attttctgcg tcagcatttc ccaatgaaac ctcaactttt ttagcctttt
     4261 gttgttcatc cacaatccag acataatttt tactatcatc cattactaga ctgctaacag
     4321 gaataagaat agccttagtt ttgcttttaa cctcaatgtt gacagaaaaa ccttgtttca
     4381 aatcaccaac ctcgcctgtc acatcaatag tataagggta tttagaacct gtattattcc
     4441 cggctgctgg actagctgct tcaccattgt ttttaggata gtcagaaata tagcttaatt
     4501 tcccagtcca ttttttatca ggatacactt tagaagtaaa gcttacttct tgacctacag
     4561 aaaggttggc tagattgtac tcagacaatt ctcccttgac ttgtaaattt tcattgctga
     4621 caatatgaac cataacttga ctcgcccctg ttggagattt agaaacattg ctattgactt
     4681 cgactacagt tccctctagg gtactgagaa cagttgttgc atccaattga ctttgagcct
     4741 tgcttaattg cgctgcagca tctgcacgcg catcacgggc atcacccaat tgagcatcaa
     4801 tagaagcaac agaatttcca gccactggag ttgggctttg caccgttgca tcttctcctc
     4861 ctactggcgc tggtaactgt ggagcctgag ctgaagcggc ttcatttcgt gcttgattga
     4921 gttcattgat atgacgatct gccttagcta ctgctcgact agctgaatca taggccgcct
     4981 gcgcttctga actactgtac ttgactaaag cctgcccttc gctgacctta tcgcccacag
     5041 aaacaaggat ttcatctaaa tcacccttac tagcatcaaa ataaacatat tgttcatttt
     5101 ttgctgttac tgtccctgac aataaaacag aggatgccac gcttccttcc ttggcaacaa
     5161 caagatgagt aggctcatct tttacagcag tctgagaagg ttgtctaaaa agtaaaatcc
     5221 ccccggcacc caatacaact acactcgcag caccgattgc tgcatacagt tgccactttt
     5281 tagctttacc attctttttc ttcataatga aactcctttt cttttttaca atactttgct
     5341 attataccaa atttccctcc agcaaacaat gcagttcaga tggaaataat ggcagttcag
     5401 gattaaacaa tcgttcggaa ttttgctttt ctattgtact agatatataa tacactatct
     5461 tgacttattt ggcaagcttt tttgcaattt tttgtgcgta gcagattttt gcaatcaaat
     5521 caaaaaaaga ataaaatata actatcaaaa aagacactct actatttaaa agattacaaa
     5581 ggagttttca atgagagaat atgatatcat tgctatcggt ggaggtagcg gaggaatcgc
     5641 taccatgaac cgtgctggtg aacatggagc caaagcagcc gttattgagg aaaagaaatt
     5701 aggtggaacc tgtgtcaacg tcggttgtgt tcctaaaaaa atcatgtggt acggggcgca
     5761 aatcgctgag actttccatc aatttggaga agactacggc tttaagacta ctgatcttaa
     5821 ctttgacttt gcaaccctac gtcgcaatcg tgaagcctac attgatcgcg ctcgttcttc
     5881 ttatgatggt agttttaaac gcaacggtgt agacttgatt gaaggtcatg ctgaatttgt
     5941 agattctcat actgtaagcg taaatggtga actgattcgt gctaaacata tcgtgattgc
     6001 tacaggtgcc catccaagta ttcctaatat tcctggtgct gagctaggtg gctcttctga
     6061 tgatgtattt gcctgggaag aacttccaga gtcaattgcc attctaggcg ctggttatat
     6121 cgccgttgaa ttagctggcg tactccacac ttttggtgtc aagacagatc tctttgttcg
     6181 ccgcgatcgt cctttacgtg gttttgattc ctacatcgtt gaaggtttgg tcaaggaaat
     6241 ggaaagaaca aacttaccac ttcacactca caaagtccct gtcaagttag aaaaaactac
     6301 tgacggcatt accattcatt tcgaagatgg tactagtcac acagctagcc aagttatctg
     6361 ggctacaggt cgccgtccaa acgttaaggg cttgcaactt gaaaaagctg gagtgactct
     6421 gaacgaacgt ggctttatcc aagtggatga ataccaaaat actgttgttg agggaatcta
     6481 tgctctaggt gatgtaacgg gcgagaaaga actgactcca gttgcaatca aggccggacg
     6541 taccctatct gaacgtctct ttaacggaaa aactactgca aaaatggatt actcaactat
     6601 tccaactgtt gtcttttcac accctgctat cggaactgtt ggtttgacag aagagcaagc
     6661 tattaaagaa tacggtcaag accaaatcaa ggtttataaa tcaagctttg catctatgta
     6721 ctctgcttgc actcgcaacc gtcaagaatc ccgtttcaaa ctcataacag ctggttcaga
     6781 agaaaaagtt gtcggacttc atggaattgg ctacggcgtt gatgaaatga ttcagggatt
     6841 tgccgttgct atcaaaatgg gagcaaccaa ggctgacttt gatgcaactg tggcaattca
     6901 cccaactgca tctgaagaat ttgtaaccat gcgttaatcg aaacaaaaga agctgatata
     6961 aatatgtctg cttctttttt aatgattttc gaatcaacaa aaaaattatc caacttgact
     7021 agtcacgaaa atcggctgtt acagtttttg ttaccttgtt tttaaaaaat ggttgacatc
     7081 gactcctata tgagtataat agttatcata cattagaaaa gaggttaact attttgaaaa
     7141 aagctcacgt ttatgctatc cctgctattg gggctgctct cattgctgct ttggcacaaa
     7201 tcagtcttcc aattggacct gttcccttca ctctgcaaaa ctttgcaatc ggcttgattg
     7261 ctactgtctt tagaccgaga gaggctgtac tttctgctgg actctatctt cttctaggtg
     7321 ctatcggtct tcctgtcttt gcaggaggtg gagctggttt tcaggcttta gttggcccta
     7381 ctgcaggcta tctttggttt tatctcgttt actctggact tacttcctct ctaaccaaca
     7441 gcaagagtgg tgttgttaag atttttcttg caaacctctt gggtgatgcc cttgtctttg
     7501 tcggcgggat tctcagcttg catttcctag ctggaatggc atttgaaaaa gctcttgctg
     7561 tgggggttct tccctttatc attccagacc ttggcaaact tctggctatt agttttatta
     7621 gccgtcccct acttcaacgc cttaaaaatc aggcttactt tactaactaa aaaaggatat
     7681 cgagttatca tgactcaata tccttttctt ttattttgaa aacttatact caatgaaaat
     7741 caaagagcaa actagaaagc tagccgcagg ctgctcaaaa cactgttttg aggttgtgga
     7801 tgaaactgac gaagtcagta accatacata cggcaaggca aagctgacgc ggtttgaaga
     7861 gattttcgaa gagtattagt tgcctttaaa ggcatccacc atcgtttgaa attcttcatt
     7921 tgagagagta atccctttgc ccattttagt atggtctgga ctccaagcac gaatatcaaa
     7981 ctttgcaggg gcaccattaa agctcacacg gttaatttcc ttggtccaac ctttttcgtt
     8041 ttcagaaaga gtcaacaagt gctcttcgat ttcaaatgta aattctgcca ttttcttctc
     8101 cttttttagt ttcattagtt tattcgtaaa atcttgtaga ttttaggaaa attttatata
     8161 atattgatat aaaagaaggg aggccaatat gagacataaa ttccagcaag ttctaaataa
     8221 aatacatgat tttttaaatg gatatgacca acctgaccag actgaaacca actcccttac
     8281 agccactatt gaagaggcta tccagaaaca aaccgctgtt caccttatct tgtctgagac
     8341 aagttttaca ggtgatatca tcaaatatga tcagcaaggc cagcaaatta tcgtgaaaaa
     8401 ttttgccaaa aatgtgagcc ggattatccg tataagcgat attcaacgcc tgcgatttgt
     8461 cccctcaact gtccaaacag cccaaaaaaa tagatttaag aaagagtgag atgtagttgc
     8521 ttcatcccac tcttttttct tagcgaattt gttcaaaatg taaatgaact gcaatatgat
     8581 ctccataacc acttctttcc aagtcacgtt gtaaacgata ggaaatgtag tgttctgcaa
     8641 tggtaatgta acctgcgccc aataaacgat gttcaaccat agattgaatc atactgatag
     8701 tcgcacgttc caccttggct tcttgtaaat ccaaaactac cttcttagtg acttgagcaa
     8761 gattttgacg caaatcatct gtcaaaacat aaacagtttg ggctgccttc aagatggctt
     8821 ggtaaatctt atctggatta aattcagcaa tttcgccatt acgtttgatt acttgcatag
     8881 gtttctcctt tattctttgt tttctttgat ttctgccagc attttttctt cttctactgt
     8941 cagttgataa tgttcaagta aatccggtct gcgctcgtag gttttcttta aactctcgta
     9001 caatcgccac tgacgaatct tttcatggtg gccactcatc aatacatctg gcacgaccat
     9061 gcctcgataa tcatagggac gtgtgtactg aggatattct aaaagacctg aagaaaaact
     9121 atcatcttgg tggctagact ccttgccaat cacttctgga atcaggcgaa ctgtagcatc
     9181 aatcatggtc atagctgcca attctccacc agtgaggaca tagtcgccta gggaaatctc
     9241 atctgttacc aaggtcttaa tgcgctcatc ataaccctca tagtgcccac agataaagat
     9301 tagctcttcc tcttgagcca aatcttcagc ataagcctga tcaaactgct ttccagcagg
     9361 atcgaggaga ataacgcgcg gatttttctt ttcaatagca tcaaaggaat cgaaaatagg
     9421 ttgtgctctg agcaacatgc cctgaccgcc tccgtagggc tcatcatcta catgacgggc
     9481 cttttcagca ttttctcgaa aattatgata ctggatatcc aagagccctt tttctcgagc
     9541 ctttccaacg attgagtgct ccagtggaga aaacatctct ggaaagaggg ttaaaatatc
     9601 aatcttcatc gtctaaccct tctaagattt ccacatcgac ccgtttattt ggaatatcaa
     9661 cattgagaac cactggtggg atataaggta aaagcaaatc acgtttgcct tttcgtttga
     9721 ccacccagac atcattagca cctggttgca ggatttcctt gatggttcca accaagctat
     9781 caccctcata gacttccaaa ccgataatct cgtgatagta aaattcacca tcgtctaggt
     9841 cattcaaatc ttcctcagcg accttgagac tatatctctt gtacttttcg atagtattga
     9901 tatggtacat atctttgaat ttaataatgt caaagttctt ctgtttacgg tggctagcga
     9961 tggtcactgt ttggacaaac tgatcttttt catcaaacaa agccagctca gctccttttt
    10021 taaaccgttc ttctgcaaaa tccgtcacag acaagactcg catctcaccc tgtaatccct
    10081 gcgtattaac gattttccca acattaaagt agttcatctt gtctcctgta atctcctttt
    10141 ttccatctta ttctgacaat tctcgaataa tagccgcaat tttttccgat tctgaccatt
    10201 gtaaataatg gtgattccct cctaaaatga gtttagtatt ggaagtccaa tattctgatt
    10261 ctctgtactc tttttctcta taaggctgac aaaaaacaaa tacaggaata tgagcttcta
    10321 tagatacatc ctcaaaatct tcctcagtaa tctctccaga tatctgaaat tctggatctt
    10381 gattttccaa ctctaagcct ttttcttgca ttaattccca gattttttta ttcgtttcag
    10441 gactaaatgt tgcttgagtt aagttcttaa aataaagttc aggaccacac tcgtcaatca
    10501 gcctcatctg ctcttccatt tctggataag gattttctga aaaatcagca aacatgactt
    10561 ttttagttgt cggttcaatt gctactaaag tctgacgctt aattggtttc tcgagtaatt
    10621 tgcaagctaa aattccactc caactatgtg cacaaagtat atattcagaa attcctaatt
    10681 cttcaagtac ttcataaacc gcatctgcaa gattatctag attttttcca gcttggtcat
    10741 gaatcggact cctacctgtg ttcggaaaat caattgtcaa ataaccaatt gtaggaggag
    10801 gtttttcaag tataagtgaa aaattttcat aacttggtag caaacctgct ccgtttagac
    10861 aaactagcac tttcttttgc ttttgataag taacagagag gctaccaatt tctgtagata
    10921 cttcaaacct cttcataaag aaatccactc attctatata atgaattatt aaaaatcctt
    10981 atcttttatt ttatcacgtt ccaaggattt tctcaagttg gaggaagggg acaacatctc
    11041 tactttccct tcaaaaatcc ttccaaatca tgtttatgtt ggtaattaac ggctgcggtt
    11101 ttgtctttct caaagacagt cttggtaagg tcaatatgat taatagctac gattgcgacg
    11161 gtgtagtaaa tgatatcagc cagttctctg gcaagttcct cgttcgaatc ctatcccttc
    11221 ttttcgacca gagcgcctat tcaaaacctc ggctacttct ccgacttcct ccactaactt
    11281 cataaagaga ccttcatcag tccgagactg ctgttaatgt tcgattaagt agtcttggaa
    11341 ttgcctaaac gttcaatctt ttatagtata ttgaaactag aatagtacac ct
//
LOCUS       DAVBCW010000060        11084 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVBCW010000060 DAVBCW010000000
VERSION     DAVBCW010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11084
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.060"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(6..890)
                     /locus_tag="VQ514_001365"
     CDS             complement(6..890)
                     /locus_tag="VQ514_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HET2500116.1"
                     /translation="MRAKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDMIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            complement(894..1280)
                     /locus_tag="VQ514_001366"
     CDS             complement(894..1280)
                     /locus_tag="VQ514_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HET2500117.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLISLMLEEQILTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            complement(1273..2616)
                     /locus_tag="VQ514_001367"
     CDS             complement(1273..2616)
                     /locus_tag="VQ514_001367"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HET2500118.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYEVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEGLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFATADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            complement(2698..3582)
                     /locus_tag="VQ514_001368"
     CDS             complement(2698..3582)
                     /locus_tag="VQ514_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2500119.1"
                     /translation="MIQVRNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGELVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHIEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKDGQVIGTCTVDLSTNT
                     NYFYGLAISEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            complement(3594..4211)
                     /gene="cysE"
                     /locus_tag="VQ514_001369"
     CDS             complement(3594..4211)
                     /gene="cysE"
                     /locus_tag="VQ514_001369"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HET2500120.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFIW
                     KHGFKLLARMHSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            complement(4227..6440)
                     /gene="pnp"
                     /locus_tag="VQ514_001370"
     CDS             complement(4227..6440)
                     /gene="pnp"
                     /locus_tag="VQ514_001370"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET2500121.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRTMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDDQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVT
                     AVYEEKYADHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRLELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHHKDHKPKKEFTETPKDSE"
     gene            complement(7125..7991)
                     /gene="metF"
                     /locus_tag="VQ514_001371"
     CDS             complement(7125..7991)
                     /gene="metF"
                     /locus_tag="VQ514_001371"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HET2500122.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNGNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQAPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            complement(8055..10304)
                     /gene="metE"
                     /locus_tag="VQ514_001372"
     CDS             complement(8055..10304)
                     /gene="metE"
                     /locus_tag="VQ514_001372"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HET2500123.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVAAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVASDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            complement(10565..10831)
                     /locus_tag="VQ514_001373"
     CDS             complement(10565..10831)
                     /locus_tag="VQ514_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2500124.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGVSQPVIARMETGESSLQLDTVLKVLTSLGKTLAVVPLEQGKS"
     gene            complement(10915..11084)
                     /locus_tag="VQ514_001374"
                     /pseudo
     CDS             complement(10915..11084)
                     /locus_tag="VQ514_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         3095 a         2539 c         2192 g         3258 t
ORIGIN      
        1 cttctttaca aaccaactat agttgacaaa gaacctaaaa tcaattgata acacaaggtc
       61 aggtcggtca actctttcaa ctgaagccct gtcaactctt cccatttatc aatcttgtat
      121 tggagagaat tgcggtgcag atagagttgc tgggctgttt tagtgagaac agcactattt
      181 tcccaaagag agagaatgat ttcctgaatc tgatcttgat ccaaaatcat ctggtgtaga
      241 cattctttaa ttggcttcaa gtccacgagt ctttctccca tactccaaag atagagctga
      301 gaaaaagtat gaacaccttg gtgaccctga cgccaccatg tcttgaacaa atcccgctca
      361 gctttgatta agtctgatag ggcttgatgt cctgtctgag accaaacctg acccaacatg
      421 atagaaagac gaagtccaaa gtcatactca actgcttcaa tcatatcact taaaatatct
      481 cttacagaag tgtatttgtc ttgttgaagc acgaaaacat aatcctgagc tccgacctgt
      541 aacactgtct gacaattcgg aaaaagagtc cgcatcatat ctagccaaga agccagattt
      601 tcctgctgaa aataagaaag atggcaataa accaactgaa tctttttaaa aacttgcggt
      661 gcctgtccct tgccttcaac cagataggaa taccaagggt ttagcgaacg agcctgctcc
      721 tgctgggtca aaagggcaac caactgcttt tcacgctcgc tgagcccagc ttcctccagc
      781 aaaatccact gctgagaagc taaaggaagc gtgagatagc cctctttctc tactggttgg
      841 tctgaaatcc gagcctcagg aaaccagtct tgtagttctt ttgccctcat gttctagccc
      901 tccacttttt ggatgcacca tgaaaccaaa ctctcaagac gttccagatt ctcagtcata
      961 tggagatagc ccataactgc ttcaaatccc gtggacatac gataagtcac gacatctgca
     1021 tttttagcct ttgtgtgact attggtattg cggccacgtt tgtagatttc ttcttctttt
     1081 tccgttagga tctgctcctc caacatgaga gaaatcaggc gagcttgagc cttggctgac
     1141 acgtacttgg tcgcctcttg gtggagttta ttgggcttgg tcataccttt gaggatgagg
     1201 tggcggcgaa tatacataga atacaccgca tctccctcaa aggctagcgc aatcccgtta
     1261 atgagattga catcaatcac gtgtccacct cactccatcc ttggtatcaa ggagcttaat
     1321 tccttgagta accaattggt cacggatttg gtctgctgtc gcaaagtcac gattggcacg
     1381 cgcctcttgg cgtttttgaa tcaagtcttc aatctctgca tccaaaactt cctcaacaaa
     1441 gacaattcca aaaatttcta acatatctgc aagagcttgc ttgacacttg catcatagtt
     1501 ccctgagttg atccatttgg ccatttcaaa gacaactgtg ataccgttgg cagcattaaa
     1561 atcttcatcc atagctgcta caaacttatc tttaaagttt tgtaactctt gggcatccac
     1621 gtttcctgta aatggttgtt cgtaagtatt cttcagatac ttgagattgg tctcggcatc
     1681 gcgaactgcc ttttccgtga agttgatagg cttacggtag tgctgggtcg caaagaagaa
     1741 acgaagtact tgcccatcaa gagttttaag ggcatcgtgt accgtaatga agttacccaa
     1801 ggacttagac attttgacat tgtcgatatt gacaaagcca ttgtgcatcc agtagttagc
     1861 aaaagccttg cctgttttag cttcagattg ggcaatttca ttggtgtggt gtggaaactc
     1921 taggtcagct ccaccaccgt gaatatcaat ggtatcacct aaaatctctg tcgacatgac
     1981 tgaacactca atatgccaac ccggacgtcc aggtccccaa ggactatccc aagaaatctc
     2041 acctggtttg gaagatttcc atagagcaaa gtctacagga ttttccttac gagccgtttc
     2101 ttcatcggta cgacctgaag cacctagctc caaatcttcc aaggttttat tagccaattt
     2161 agcatagttg tgggattttt ctacacggaa atagacatcc ccttgactct cataggcaaa
     2221 gcctttctcg atcaagcctt ccacaaaacg gatgatgtct gccataaact ccactacacg
     2281 cggatggcga gtcgcaggtt tcacgcccaa ggccgtcaca tcctcacgaa aggcagcgat
     2341 gtacttatcc gcaacctcct gaggcgtgat gccttcttcc ctggcacggt tgataatctt
     2401 atcatccaca tccgtaaaat tggaaatata ggcaacctcg tacccacgat actcaaaata
     2461 gcgacgaatg gtatcaaaag ctaccgttga acgagcattc cctacatgaa tatagttgta
     2521 caccgttggc ccacaaacat acatcttgat cttgccgtcc tcaatcggga caaattctcg
     2581 caaatcacga gacatggtgt catagatttt aatcataaat cataatcaag aaagctgaaa
     2641 tccaagaaca attagtttca tcactaaaag ttcaagtaaa tttcagtccg aatatctcta
     2701 cacttcggaa tcccttgctc ctttctcatt cagataaacc acctgagtct gtttgacaaa
     2761 gccaattttt tcatacaaac gtttggcacc tacattgcta tcttccactg caatctgaaa
     2821 ttccttgtca ttttgctcaa ttagttggtt gacgagggat tttgctaagt agcttccata
     2881 gcctttccca cgttcaggtt ccgatattgc taaaccgtag aagtaattcg tattagtcga
     2941 taaatctacc gtgcaagtcc caataacctg accgtctttt aacaaaatat atagtcggct
     3001 ttctggatcc ttcagagctt cagcgacata tctatccaca acttctctcg attcatgttc
     3061 ctctgaaaat gcctgaaatt ttaattgact aatttgatcc tgatacgaac tatctgctaa
     3121 caaaacttca atatgggaaa catttgctaa cggataaggt cttctatcct tacctaacca
     3181 agtttctgtc tcttcatcct cgattagtcc ccagttactg gcaaagtcag gatgattctc
     3241 taaaaaaata cgttctgtct gaaaagtgac tgatcgaatg gggaaagaag ctgtttctct
     3301 ctcaaaacta gtaaacaatg cacgcgcaat cccctgacgg cgatgacctg gatgaaccag
     3361 tatcgtcact tctacatctt ggtcatctgc atagacagtt aataaaccaa caagttcgcc
     3421 tttttcataa taaaggaaaa aggcgggcat gtttgggtca aaattaagca tgttagagag
     3481 atagggatcg cgataggtac cgtcatagtt ttggcaacag ttaattactt ttttcgcctc
     3541 agatagctcc tcttggctta acttgtttct tacttgaatc atataggtat cctctacaaa
     3601 ccagacgatc tgtgactggc atctttagcc tgctcgagtt tattgacata atactctcgt
     3661 ttttcttcga cttcgtgaat gacaggctca tctttcttac catgaagacg gacaatcttg
     3721 gccggaatac cgacaaccgt cacatcacta ggtacatctg ctacgacaac tgctgcagca
     3781 ccgaccttgg cattttcacc aatttccaca ggtccgataa cttgggcatg ggctgatatg
     3841 agggctccct ttcgtacagt cggatggcgt ttgccacagt ctttccctgt tcccccaaga
     3901 gtcactccgt gatagagaag aacgcctttt tcaacaatcg ctgtctctcc aatcaccaga
     3961 ccagaaccat ggtcaataaa gacacctgaa tcaatctggg ctcctggatg aatctcaatc
     4021 tgagtccaaa agcgccaaaa ctgactgtgc atacgagcta agagtttgaa gccgtgcttc
     4081 cagataaaat gcgagagacg gtgggccgcc aaggccttga cacctggata agtcagcaaa
     4141 acctccaaag tggtgcgggc cgctggatca ttttctttta cgatatcaat ggtttcgcgc
     4201 caccacccca tacatttctc cttttcttat tctgaatctt ttggtgtttc tgtaaattct
     4261 ttcttaggtt tgtgatcctt gtgatgacgt gggcggtgag gacgctcaga cttttcacct
     4321 ttttcatcat gctcaggttt tggcggacga ggtagaagag ccttcataga ggcatcgata
     4381 cggccttttt catcaatctt gataacctta acatcaactt catccccgat ttctaccaaa
     4441 tcctctacac gattggtacg agtccaagcc atctcagaga tatgaacaag ggcatctgtc
     4501 ttatcaaaga ggttaacaaa ggcaccaaat ttctcgatac gaacgacttt agcacggtaa
     4561 acttcatcca ctttggcttc acgaaccaaa ccagcaataa tttctttggc acggttaata
     4621 gcatcttggt cactagagta gatagacaca tttccttctt cgtctatatc aatcttaaca
     4681 cctgtttcag cgataatctt gtcgatggtt tctccaccct taccgatgac aatcttaatc
     4741 ttgtccacat caatcttgat cgtatcaatt ttcggagcag ttggagccaa ttctagacga
     4801 acttctggaa tggttgcttc aatgacatca aggatttcaa aacgcgcttt cttggcttga
     4861 gcaagagcct ccgtcaagat ttctgcagta atcccttgaa tcttgatatc catttgaagg
     4921 gctgtaatcc catcacgagt acctgcaacc ttgaagtcca tatctccaaa gtgatcttcc
     4981 aaaccttgga tatctgtcaa tactgtgtag ttatttccat ctgagataag tcccatagca
     5041 ataccagcta ctggcgcctt gattggcaca ccaccagcca taagggcaag agttcccgca
     5101 cagatagaag cttgagatga agaaccgttt gattccaaaa cttctgctac tagacggata
     5161 gcgtatggga attcttccaa gcttggcaag acttgagcaa gagcacgctc accaagggca
     5221 ccgtgaccga tttcacgacg acctggcgca ccgtaacgac ctgtttcccc tacagaatat
     5281 tgagggaagt tatagtggtg cataaagcgt ttcttgtact ctggatccaa accatcaatg
     5341 atttgagttt ctcccatcgg agccaaggtc aagactgaaa gagcttgagt ttgcccacga
     5401 gtaaagagac ctgaaccatg tacacgagga aggaagtcaa caaccgcatc caaaggacgg
     5461 atttcatcga ccttacgacc atcaggacgc accttgtctt ctgtaattaa acgtcgcact
     5521 tctgcgtgtt ccatttgttc caagatttca gccacatcac gcataatacg gtcaaattct
     5581 tcgtggtccg catatttttc ttcgtaaacg gcagtcactt ggtctttcac tgcttgagtt
     5641 gcagcttcac gggccaattt ctcttctact tgaactgcct tttggaggtc actgttgtag
     5701 gctgcaatga tttcagcttg caattcagca tccacgtgaa gcaattccac ttctgctttt
     5761 tctttaccga cagcagcaac gatttcttct tggaaggcaa tcaattcttt gacagcttcg
     5821 tgccctttaa gaagcgcttc caacatgatt tcttctgaca attctttggc accagactct
     5881 accatgttga tagcgtgctt agttccagct actgtcaatt caagaagaga ttgctctgct
     5941 tgttcttgac ttgggttgat gatgatttgg tcatctacat atcccacttg taccccagca
     6001 attggtccgt caaatggaat atctgaaata gacagtgcca aagatgaacc aaacatagca
     6061 gccattggtg cagatgcatt ttcatcataa gaaagcactg tattgatgac ttggacttca
     6121 ttacggaaac cttccgcaaa catagtacga atcggacggt caatcaaacg cgctgtcaag
     6181 gtcgcatctg ttgaaggacg tccttcacgt ttcataaagc caccaggaaa cttcccagcc
     6241 gcatacattt tttcttcgta gttgacttgg agtgggaaaa aatccccagt tgccattttc
     6301 ttagacataa cggcagcagt caagacagtt gactcaccgt aacgtacgac aacagagcca
     6361 tttgcttgct tagcaacctg accagtctct acaattaact cacgacccgc aaaagtcgtt
     6421 tgaaacactt gttttgccat tttaatcccc tttggattga tgaaattata cgccttgcct
     6481 acaaagatca agataccaag gacgtcaaaa gcaaagtaaa aataggaaac tgacgaagtc
     6541 ttcgatgaag acaagacagt ttatcttttt tacacagctt ttcggccgtg ttcaattaca
     6601 caagatattt tggacggttc ggcttgccga acatttctgt agaaaaatag gaaggtgacg
     6661 tcgcactcga cgagtgctag gaagcttatc ttttttccta agaaatgaga ccaaaattca
     6721 agtcatcaag ataccaagcc gtcaagcaac tcaaaggaaa ataggaaatc gaacgacgga
     6781 gcgactgctc ctaggcagat ttatcttttt ccacagagtt gtaggcgtgt tcagttttca
     6841 agatacatca ttagaaaggt ttaatattaa agtatctaaa cctttcacgc taatcgctat
     6901 cgggcgatta gctaaatgct ttactaactc tttcgtcaaa taacatcgat ttgactcact
     6961 cgtgtcgtta aatcttacag tttaaatgca ttgtattatt taataccttc atctttgtat
     7021 caagtacgta cagaatctat tttatcatat ttttcttaaa aagtgaggtc tttaccatta
     7081 aaaaggaacc attcccctca cctgagaaga atggtttgct tttattatcc tagagactgg
     7141 tgattaaaca aggcatgggt tgcttgatgg atgtattttg ctgtatcagc attattcatc
     7201 gtatagagat gcacaccggc aacatcctga gttaccaagt ccacgatttg gtccactgca
     7261 taggcaagtc ctgctgctct gagcgactca gggtcatgct catacttgtc taagatggct
     7321 ttaaatttgc gtggaagatg gatattctca caagtcttca agagtcggag agcctgattt
     7381 cgattcagaa ttggcataat tcctgcatga ataggaacat caatcccagc caagatacac
     7441 ttgtcctgaa aatcatagaa gcgctcattg tcaaagaaga gctgagttac gaggctcgaa
     7501 cagcctgcat ccactttctt cttaagattt tgaatatctg aaatctgatt tggcgaatct
     7561 ggatgccctt ctggatagca agctccaata atatcaaagt gaggggcttg ttccttgata
     7621 aactcaatca agtcggttgc atagcggaaa tccttttgtg gttctacgtc tggaataata
     7681 tccccacgaa gagccaagat tttctgaacc ccaactttgt ccaagtcagc aatagtttca
     7741 gcaaccttgt ccttagttag ataaatagct ggcaagtggg caatggtcgg aatcgccaag
     7801 tcattttgga taaagtcagc caaacgaacc gtcgtttcct tgatattaaa tttattattg
     7861 ctggcagtta cactgataaa atggggagcc aactcctgca tatcctgcaa ggctgaaata
     7921 atgttaccat tacccacggc tgggtttgga gggaacactt caaatgagag tgacggtgtt
     7981 tggcgtgaca tatgtaataa ccttttctag ttgatttctt tttgaacaac cactgtatgg
     8041 agagaaatcc aatcttacaa tttctcacgc gcagctttag ctgcttcaac aaggcggatc
     8101 aagctttctt ttgtttctgg gataccacgt gttttcaaac cacagtcagg gttgatccaa
     8161 actttcttgc ttggcacttt agcaaggatg gcttcgattg tgttgtcgat ttcgccttca
     8221 tttggtacac gaggtgagtg gatatcgtaa accccaggtc ccacttctgt ttggaagttt
     8281 ttcgctttga gttcgtccaa gatttcaagg tttgaacggc tggcttcaaa ggaaataacg
     8341 tctgcatcca tgttatcgat agctgggatg atatctgtaa attctgagta acacatgtga
     8401 gtgtggattt gtgtgtctga cgctactgtt gagtgtacca agcggaaggc aggaattgcc
     8461 cagtcaaggt aatcttcgta ccagtcacta cgacggagtg gcaatttttc acgaagagca
     8521 gcctcatcaa tttggatgat tttcacacca gcagcttcaa ggtcaagcac ttcatccttg
     8581 atagcaaggg cgatttgaag agtagaatcc ttgatagaga tgtcttcacg tgggaatgac
     8641 cagttaagga tggtaacagg tccagtcaac atacctttaa caggtttgtt tgtacgactt
     8701 tgtgcatagc tagaccattt aacagtgata gggttaagac gagtgacatc accccagatg
     8761 attggtggtt ttaccccacg cataccgtat gattgtaccc atccattttt agagaagagg
     8821 tatcctgaca agttttgacc gaagtactca accatgtcat tacgctcaaa ttcaccgtga
     8881 acaaggacat caaagtcaat atcttcttgc cacttgatcc attcgtcaat cgtttcagca
     8941 aggaaagcat cgtactcttt ttgagacaat tcacctttac ggtaagccaa acgtttggca
     9001 cgaacttctt ttgtttgagg gaatgaacca atcgttgttg ttggaagagc tggaagtttg
     9061 aaagcttctt cttggatagc ttcacgttct gcaaaggctg gcaaacgagt gtagtctgcg
     9121 tctgtcaagc cagcgatacg cgcacgaagt tcagcatttt caccaacacg ctcagtcgca
     9181 aagagttctt tgttggctgc aagagcttct gaaccttgac cattgcggat agcatccaag
     9241 tcacggattt catccaattt ttcaactgca aaggcaaagt ggttcaagag tgctggttca
     9301 aattcttcat tagcagttgt aaatggcaca tgaagaagtg agcaagagct tgtcaaaacg
     9361 atgttttcag ctggaatttg ctcaagaaca gccaagctct tttcgtagtt gttgcgccag
     9421 atgtttttac cattgacaat acctacatag agagtcttgt cagctgggaa gccaccttta
     9481 acgagttcaa gagttttctt accttcaaca aagtcaagac cgatagcatc tactggtaag
     9541 tttacaaggt cagcgtatac gtcacgaaca tcaccgaaat aagtttgaag caagacttca
     9601 agaccttttt tgtcagccaa gagtttgttg taaaggttca agaagagagc tttttcttca
     9661 gctgtcaagt cttttacaag agccgcttca tccaattgga tgcgagtcgc accaagttca
     9721 gccaatttag caaaaacttc ttggtaagca gccactaagc tatctacgaa gtcgtctgct
     9781 ttcacgcctt cttcaaagtc tgacaattga aggaaagtga agggacctac aagaacagga
     9841 cgagtgttca atccaagttc tttggcttct tggaactcat cgaaaatctt gtgaccagcc
     9901 aattttactt gagtgtcttt ttcaaattta ggaacgatgt agtggtagtt agtgttgaac
     9961 catttcttca ttggaagggc gcgaacgtcc cctttttctc cctggtaacc acgtcccaaa
    10021 gcgaagtagc gctcaaggtc agacaagtcc aagttttgaa cggatgcagg caccacgttg
    10081 aaaaggaaag ccgcatctag gaagttatca tagtgagaaa agtcatttga tggaatttca
    10141 gtgatgcctt tttctttgac aatgttccag tgtttagcac gcaagtcttt tgctgctgct
    10201 aaaagttctt cttctgagat ttcttttcta aagtattttt cagttgtaaa ttttaattca
    10261 cggaattcgc ccaaacgagg gaaaccgatg attgtagttg acatgatgtg tcctccaaaa
    10321 tttgttgttg aaactatctt aacagaaaag aaagcgtctg tataattgta aaaaattagg
    10381 gtttgatata gtttgaaact atatatctgt ttcggacaaa agaaaaagac ttgaagcaaa
    10441 cgtctcaaat cctttgtaat tcttacttta cagctatatt ccaattagaa tactaaaaca
    10501 tgttattagt aattcttata agtgactatg acctgttatt agaaaagact ataactgatt
    10561 ctagtcaact tttcccctgt tcaagtggaa cgactgctag tgtctttcct agactggtta
    10621 gaacttttaa gaccgtatcc aactgaagac tgctctctcc tgtttccatc ctagctatga
    10681 caggttgact cactccactg acttcttcca gctttttctg actgattcct tgttcatacc
    10741 tagcctcaat caactcgctc atgatagcca ctcgcatatc actttcaagg atttcctcct
    10801 tgctaaagag ctcagatgga catccttcca attactccca atagcactat tcttcatcac
    10861 ttaaccctct ttcttttacg tctatgtatt tttaaaagaa tgagcgaatt atgattcgat
    10921 agattgacca gcaggtttaa agttggtgct agcctgtttc ttaagcgctt ttccttttct
    10981 agggtaaagc agatcctgct tgcttaaccc caattttcca cgatgaatcc aatagtaaat
    11041 ggttgaaatt cccacgttaa cccctttagc catcaccatc attt
//
LOCUS       DAVBCW010000061        10989 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVBCW010000061 DAVBCW010000000
VERSION     DAVBCW010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10989
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.061"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            136..2316
                     /locus_tag="VQ514_001375"
     CDS             136..2316
                     /locus_tag="VQ514_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HET2500125.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFTPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDTSQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKNLTEEVYSVADGQVVGLEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            2368..3183
                     /locus_tag="VQ514_001376"
     CDS             2368..3183
                     /locus_tag="VQ514_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET2500126.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETLVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAMLNWK"
     gene            3323..4666
                     /locus_tag="VQ514_001377"
     CDS             3323..4666
                     /locus_tag="VQ514_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2500127.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLASNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLEIVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            5043..6233
                     /locus_tag="VQ514_001378"
     CDS             5043..6233
                     /locus_tag="VQ514_001378"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HET2500128.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            6786..7721
                     /locus_tag="VQ514_001379"
     CDS             6786..7721
                     /locus_tag="VQ514_001379"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HET2500129.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            7755..8792
                     /locus_tag="VQ514_001380"
     CDS             7755..8792
                     /locus_tag="VQ514_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET2500130.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQVLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNSSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKANSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            8965..9570
                     /locus_tag="VQ514_001381"
     CDS             8965..9570
                     /locus_tag="VQ514_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HET2500131.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            9726..10256
                     /locus_tag="VQ514_001382"
     CDS             9726..10256
                     /locus_tag="VQ514_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HET2500132.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKEQRHFVIRQK"
     gene            10277..>10989
                     /gene="rlmN"
                     /locus_tag="VQ514_001383"
     CDS             10277..>10989
                     /gene="rlmN"
                     /locus_tag="VQ514_001383"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HET2500133.1"
                     /translation="MKPSIHSLAHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQGIVQESADGTVKYLFELPDGMLIETVLMCQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFDERGQDERISHIVVM
                     GIGEPFDNYNNVLNFFRTINDDKGMAIGARHITVSISGLAHKIRDFADEGVQVNLAVS
                     LHAPNNELRSSIMKINRAFP"
BASE COUNT         3341 a         2017 c         2395 g         3236 t
ORIGIN      
        1 tagaaatttt ttagaaaaaa gtgtaatttt tttataaaag cctttacaaa aaaaattaaa
       61 gtggtataat taaatcatag aaggtgcaaa cgttttcgta aaacgtttgc ctaaataaaa
      121 ataaaggaga tttgaatgat gaaagataca ttcaaaaatg tcttgtcttt cgaattttgg
      181 caaaaattcg gtaaggcttt gatggtagtt atcgcggtta tgccggctgc tggtttgatg
      241 atttcaatcg gtaagtctat cgtgatgatt aacccaacct ttacaccact tgtcatcaca
      301 ggtggaattc ttgagcaaat cggttggggg gttatcggta accttcacat tttgtttgcc
      361 ctagccattg gaggaagctg ggctaaagaa cgtgctggtg gtgctttcgc cgctggtctt
      421 gccttcatct tgattaaccg tatcactggt acaatttttg gtgtatcagg cgatatgttg
      481 aaaaatccag atgctatggt aactactttc tttggtggtt caatcaaagt tgctgattac
      541 tttatcagtg ttcttgaagc tccagccttg aacatggggg tattcgtagg gattatctca
      601 ggttttgtag gggcaactgc ttacaacaaa tactacaact tccgtaaact tcctgatgca
      661 ctttcattct tcaacgggaa acgtttcgta ccatttgtag ttattcttcg ttcagcaatc
      721 gctgcaattc tacttgctgc tttctggcca gtagttcaaa caggtatcaa taacttcggt
      781 atctggattg ccaactcaca agaaactgct ccaattcttg caccattctt gtatggtact
      841 ttggaacgtt tgctcttgcc atttggtctt caccacatgt tgactatccc aatgaactac
      901 acagctcttg gtggtactta tgacatttta actggtgcag ctaaaggtac tcaagtattc
      961 ggtcaagacc cactatggct tgcatgggta acagaccttg taaaccttaa aggtactgat
     1021 actagtcaat atcaacactt gttagataca gtacatccag ctcgtttcaa agttggacaa
     1081 atgatcggtt cattcggtat cttgatgggt gtgattgttg ctatctaccg taatgttgat
     1141 gctgacaaga aacataaata caaaggtatg atgattgcaa cagctcttgc aacattcttg
     1201 acaggggtta ctgaaccaat cgaatacatg ttcatgttca tcgcaacacc tatgtatctt
     1261 gtttactcac ttgttcaagg tgctgccttc gctatggctg acgtcgtaaa cctacgtatg
     1321 cactcattcg gttcaatcga gttcttgact cgtacaccta ttgcaatcag tgctggtatt
     1381 ggtatggata tcgttaactt cgtttgggta actgttctct ttgctgtaat catgtacttt
     1441 atcgcaaact tcatgattca aaaattcaac tacgcaactc cagggcgcaa cggaaactac
     1501 gaaactgctg aaggttcaga agaaaccagc agcgaagtga aagttgcagc aggctctcaa
     1561 gctgtaaaca ttatcaacct tcttggtgga cgtgtaaaca tcgttgatgt tgatgcatgt
     1621 atgactcgtc ttcgtgtaac tgttaaagat gcagataaag taggaaatgc agagcaatgg
     1681 aaagcagaag gagctatggg tcttgtgatg aaaggacaag gggttcaagc tatctacggt
     1741 ccaaaagctg acattttgaa atctgatatc caagatatcc ttgattcagg tgaaatcatt
     1801 cctgaaactc ttccaagcca aatgactgaa gcacaacaaa acactgttca cttcaaaaat
     1861 cttactgagg aagtttactc agtagcagac ggtcaagttg ttggtttgga acaagtaaag
     1921 gatccagtat ttgctcaaaa aatgatgggt gatggatttg cagtagaacc tgcaaatgga
     1981 aacattgtat ctccagtttc aggtactgtg tcaagcatct tcccaacaaa acatgctttt
     2041 ggtattgtga cggaagcagg tcttgaagta ttggttcaca ttggtttgga cacagtaagt
     2101 cttgaaggta aaccatttac agttcatgtt gctgaaggac aaaaagttgc agcaggagat
     2161 ctccttgtca cagctgactt ggatgctatc cgtgcagcag gacgtgaaac ttcaacagta
     2221 gttgtcttca caaatggtga tgcaattaaa tcagttaaat tagaaaaaac aggttctctt
     2281 gcagctaaaa cagcagttgc taaagtagaa ttgtaatata cttgaggttg gaagctgtat
     2341 tccaacctct tattttggga gaaaagaatg aaatttttaa cactcaatac tcacagttgg
     2401 atggagaaag aagcagagga aaaattccag attttgcttg aagatattct tgaaaaggac
     2461 tatgatttga tttgtttcca agaaatcaat caggagatga cctcgtcaga ggtggaggtt
     2521 aatgaccttt atcaagcttt gccagcagct gagcctattc accaagacca ttatgttaga
     2581 ctcttggttg aaaagttgtt agagcaaggg aaaaattact actggacctg ggcttataac
     2641 catatcggct atgaccgcta ccacgaaggt gtggctatct tgtctaaaac acctattgaa
     2701 gccagagaaa ttttggtttc agatgtggat gatccaacag actatcatac tcgccgtgtt
     2761 gccctagctg aaactttagt cgatggcaag gagctagcag ttgccagtgt tcatctctct
     2821 tggtgggata aaggtttcca agaagaatgg gcacgatttg aggctgtctt gaaaaaattg
     2881 aacaagccac ttttactagc tggagatttt aacaacccag caggtcagga aggttaccaa
     2941 gctattttag ctagtccatt aggcttacaa gacgcatttg aagttgctca agagaaaagt
     3001 ggtagctata ctgttccgcc tgaaattgat ggctggaaag ggaacactga accccttcga
     3061 atcgattatg tctttactac caaagagtta gcggtggaaa atttacatgt cgtatttgat
     3121 ggtaacaaga gtccacaagt gagtgatcac tatggcttga atgctatgtt aaactggaaa
     3181 taataactga aaagaggttg gaactataaa attccagcct tttcttacta gagaagctac
     3241 tggaaatagc ctaaataagt gagactactg taatggaata aaatatggta taattgataa
     3301 gatagataga atcgaggatg ttatgtcatt tacgaaattt caatttaaaa actatattag
     3361 agaagccttg aaggagttaa aatttacaac tccaacagag gtgcaagata agttgattcc
     3421 tattgttttg gcaggtcgtg acctagtagg agaatcaaaa acaggttcag gtaagactca
     3481 tactttcttg ttaccgattt tccagcaatt agatgaagct agcgatagtg tacaagcagt
     3541 gattactgca ccgagtcgtg agttggctac tcaaatttac caagtagcgc gtcagatttc
     3601 agctcactca gatgtcgaag ttcgtgtggt taattatgtg ggtggtacgg ataaggctcg
     3661 ccagattgag aaattggcaa gcaatcagcc tcatattgtt attggaacac caggccgtat
     3721 ctacgacttg gttaaatctg gtgatttagc tattcataaa gccaagacat ttgttgttga
     3781 tgaagcagat atgaccttgg atatgggatt cttggaaatt gttgataaga ttgctggcag
     3841 tcttccaaaa gacttgcaat tcatggtctt ctcagcgact atcccacaaa aactgcaacc
     3901 attcttgaaa aaatacttat caaatcctgt tatggagaaa attaagacta aaacggttat
     3961 ttctgacacc attgataatt ggttgatttc gaccaaggga catgataaga atgctcaaat
     4021 ttaccaattg actcagttga tgcagccgta tttggcaatg atttttgtta acactaaaac
     4081 gcgtgctgat gaattgcatt catatctgac tgctcaaggc ttgaaggttg caaaaatcca
     4141 tggagatatt gcccctcgtg aacgcaagcg aatcatgaat caggtgcaaa atctggattt
     4201 tgagtatatt gtcgcaacag atttagcagc gcgtgggatt gacattgaag gtgtcagcca
     4261 tgtcatcaat gatgccattc cgcaagactt atcttttttt gttcaccgtg ttggtcgtac
     4321 tggacgaaat ggcctaccag gtacagctat taccctttat cagccaagtg atgactcgga
     4381 tatccgtgag ttggagaaat tgggaatcaa gtttagtcct aagatggtca aagatgggga
     4441 atttcaagat acctatgacc gtgatcgtcg tgccaaccgt gagaaaaaac aagataaact
     4501 tgatatcgaa atgattggtt tggttaaaaa gaaaaagaaa aaagtcaaac cgggttataa
     4561 gaagaaaatt caatgggcgg ttgatgaaaa gcgccgtaaa actaagcgtg ctgaaaatcg
     4621 cgctcgcggt cgtgcagagc gtaaagctaa acgccaaaca ttttaataga aattgttgga
     4681 gtactgagct ccaacttttt tatttatgag aacgaactat ctaaaccgaa acactacatt
     4741 aaagactgca aattgcgatt aaaaatggta taatgataaa gttatatagt cccgataaga
     4801 tggtaggtat ttattacgaa gagttttcct atcagtagtt tgcaattcgc aaagaatgac
     4861 ggcttcgagg tcgctcgtct ttctacggtt gccgccatac tgcggtcacc ctatgcgcct
     4921 tactagcttc agaaataaaa aagtatcgtt tttatttctg aagctagtaa ctcttagata
     4981 atggaaattc ctatcagtac tttgtaactc tataacaata ttttttaagg ggggacattt
     5041 ttatgtcaga acgtaaatta ttcacgtctg aatctgtatc tgaggggcat ccggataaga
     5101 ttgcagacca aatttcagat gcgattttgg atgctatttt agcaaaggat ccagaggcgc
     5161 acgttgctgc tgaaacagct gtatatactg gttctgtcca cgtttttggt gaaatttcta
     5221 caaatgccta tgtggatatt aaccgtgtgg ttcgtgatac cattgcagag attggttata
     5281 ccaatacaga atatggattt tctgctgaga cggtgggagt acacccatct ttggtggaac
     5341 aatctcctga catcgctcaa ggtgttaacg aagccttgga ggttcgtgga aatgctgatc
     5401 aagatccact ggacttgatt ggagcaggtg accaagggct catgtttgga tttgcagtag
     5461 atgaaacaga agagctcatg ccattgccaa ttgcactcag tcataaattg gttcgtcgtc
     5521 tggcagaact tcgtaagtct ggagaaatta gctatctccg tccagatgca aaatcacaag
     5581 ttacagttga gtacgatgaa aatgaccgtc cggtacgtgt agatacagtc gttatttcta
     5641 ctcagcatga tccagaggcc actaatgaac aaatccatca agatgtgatt gacaaggtca
     5701 tcaaagaagt tattccatct tcttatcttg atgataagac aaaattcttt atcaatccga
     5761 caggtcgttt tgtaatcggt ggtcctcaag gggactcagg tttgactggt cgtaagatta
     5821 ttgtagatac ttatggtggc tactctcgtc atggtggtgg tgccttctct ggtaaagatg
     5881 cgactaaggt ggatcgttca gcctcttatg cggctcgcta tattgccaag aatatcgttg
     5941 cagcaggcct tgctaagaag gcagaagtgc agttggccta tgctatcggt gttgcgcaac
     6001 ctgtttctgt tcgtatcgat actttcggta caggaacagt agctgaaagt caacttgaaa
     6061 aagcggctcg tcaaatcttt gaccttcgcc ctgcagggat tatccaaatg ctggacctca
     6121 agcgtccaat ttaccgtcaa acatcggctt acggtcacat gggacgtaca gatattgatc
     6181 ttccatggga acgtttggat aaggtagatg ctttgaaaga agcagtaaaa taagatttta
     6241 agaggggaac gtcctctctt ttttatagtt tttaactata ctgggatact gttctgaaaa
     6301 tccattttgc gaaagtagag atttacatgt ataatggatt gaaactagaa tagtacacct
     6361 caacttctaa aacattgtta gaaatcgatt tgactgtcct gatcgatttc tcctgttctt
     6421 gtttcatttt actatatttc tttaaaaatg ataaaggttg agatttctcc tcgtaataga
     6481 taatcatggg gatatttcaa tccaaagttt tattcgttat cacttgacta ttgcaaggtt
     6541 ttctagagca acagagtcat ggaatggact catggttgag atttctcctt gttgcttgga
     6601 cttcattcaa aagtctgtta cccaagcctt gttcaaactt ccaatacact agctgtttcc
     6661 atagcatgac ttctgtacta gactttcttt tccgaataaa tagatagaac cacagaatct
     6721 agtaaaccta gaattaaaat tatggtataa tattagcaat aaaagaaatc tggaggatta
     6781 gaatcatggt atcaacgaaa acacaaattg ctggttttga gtttgacaat tgcttgatga
     6841 atgcagcagg tgtggcttgt atgacgatag aggagttaga agaggtcaaa aactcagcgg
     6901 caggaacctt tgttactaag acagcgacct tggacttccg tcaggggaat cctgagccac
     6961 gctaccaaga tgttccactt ggttccatca actctatggg cttgccaaat aatggcttag
     7021 actattattt ggattatctt ttagatttgc aggaaaaaga gtcgaaccga actttcttct
     7081 tatctctggt cggcatgtct ccagaggaaa cccatactat tttgaaaaaa gtccaagaga
     7141 gtgattttcg tggtctgact gagctaaatc tttcctgtcc aaatgttcca ggtaaacctc
     7201 agattgccta tgattttgag acaacagacc ggattttggc agaagtgttt gcttacttca
     7261 ccaaacctct tggaattaaa ttgccacctt attttgatat tgttcacttt gaccaagcgg
     7321 cagctatttt caacaaatat ccgctcaagt ttgtcaactg tgttaactct atcggaaacg
     7381 gcctctatat agaagacgaa tctgtcgtta ttcggcctaa gaatggtttt ggtggaattg
     7441 gtggagaata catcaaaccg actgctctag ccaatgttca cgccttttat caacgtttaa
     7501 atcctcaaat ccaaattatc ggaacaggtg gcgttctgac tggtcgagat gcctttgaac
     7561 acatcctctg tggagcaagt atggtgcagg tgggaacgac ccttcacaaa gaaggcgtca
     7621 gtgcttttga ccgcattacc aatgaactga aagcaatcat ggtggaaaaa ggctacgaga
     7681 gcttagaaga tttccgtggg aaattgcgct atattgacta aattaaatcg aaaaatctga
     7741 agaaaggaga gacgatgcta gccattgaag aaagtcagaa gttgacttta tcaaatttac
     7801 cgagcctgag cctatttaca gggacagatc agggtcagtt tgaagtgatg aagagtcaag
     7861 tgttgaaaca gattgggtat gattctgctg acctcaactt tgcctacttt gatatgaaag
     7921 aagtagttta caaggatgtg gaactggagt tggtcagcct tcctttcttt gcggatgaga
     7981 aaatcgtgat attagattat tttatggata tcacgactgc taagaaacgc tttttgacag
     8041 atgatgagct taagtcattt gaggaatacc ttgacaattc ttctccaaca accaagttga
     8101 taatctttgc agaaggaaag ctggatagca aaagacggtt agtcaaatta cttaagcgtg
     8161 atgccaaggt cttcgatgca gtagaagtaa aagaacaaga attgcgccag tatttccaaa
     8221 agtggagtca gaaacaaggt ctgcagttta ccaatcattc ttttgaaaat ctcctcatca
     8281 agtcggggtt tcaatttagc gaaatccaga aaaatcttct ctttttacag tcctataagg
     8341 cgaattctgt tattgaggaa gaggatattg ttaacgcaat tcccaagacc ttgcaggaca
     8401 atatttttga tttaactcag tttattctga ctaaaaagat ggatcaggcg cgcgatttgg
     8461 tgagagactt gaccttgcaa ggggaagatg aaatcaaact gattgcagtc atgctgggac
     8521 aatttcggac ttttactcag gtgaagattt tggcggagtc tggccaaaca gaatcgcaga
     8581 ttgcaagtag tttaggtagt tatctgggac gtaacccaaa tccttatcaa atcaagtttg
     8641 cattaagaga ttcgagagga ctttctttga gctttctgaa gcaagctatt tcctatttga
     8701 ttgagacaga ctatcagatt aagacaggtc tttatgaaaa aggtttcctt tttgaaaagg
     8761 cactcttaca gattgctagt caggtcaatt gacatttgtt gaaactacaa cccgcgtcag
     8821 gattatcttg tcgtgggttt cttgctgatt tgcttatttt attagaaagt atgtcagtga
     8881 tttccttatc tataagcaaa aaacttgaaa aaagtgaatg tttgcgttat cattttcata
     8941 tagaaagaaa aagaggtatt acagatggct attatcttac cagaacttcc atatgcatac
     9001 gacgctttgg aaccatacat cgatgcggaa acaatgcatt tgcaccatga caaacaccat
     9061 caaacttatg tcaacaatgc caatgcagct ttagaaaaac accctgaaat cggtgaagac
     9121 cttgaagcct tgcttgctga tgtagaatct atcccagctg atatccgtca agcacttatc
     9181 aacaatggtg gcggacactt gaaccacgct cttttctggg aattgatgac tcccgagaaa
     9241 acagctcctt cagcagaact ggcagcagca atcgatgcaa catttggttc atttgaagaa
     9301 ttccaagcag ccttcactgc agcagcaaca actcgttttg gttcaggttg ggcatggttg
     9361 gttgtcaaca aagaagggaa acttgaagtg acttcaacag caaaccaaga cacaccaatc
     9421 tcagaaggta aaaaaccaat cttgggcttg gacgtttggg aacatgctta ctacgtgaaa
     9481 taccgcaacg tgcgtcctga ctacatcaaa gctttctttt cagtaatcaa ctggaataaa
     9541 gtagatgaat tgtacgcagc tgctaaataa tgatagttgg agggaagaat tgttcttctc
     9601 tttttaggtt ataagattct ggtctgacaa aatcgtcaga cttttttcat ttttatgaga
     9661 aacgtgctaa ctgctagaaa ttatggtaga ataaagtatt aagtaatcgt ggaaaaagga
     9721 tatctatgcg aaaagaaatt gcacctgaat tatacaacta taacaagttt cctggtccgg
     9781 agttccattt acacggggat aaggtcgaaa cggaagggat agctttttcc ttggttgaaa
     9841 atatcaagga tgcctttgat gtgacgactt ttaatcagcg tttttcagaa gtcttaacca
     9901 agtttgatta tatcgtgggg gactggagca acgaacagct tcgcctacga ggtttttaca
     9961 aggatgaccg aacagaagaa aaacttgaaa aaatcagtcg tttacaagac taccttttag
    10021 agtattgtag ttatggttgt gcctattttg tcttagaaaa tgaagcccct aagcgagcat
    10081 catttgacaa gaaaatgcgt aagacggaag aagaaacacc ttctagaaaa ggaaagaaac
    10141 cgactcaaac taaacgaaag tcgaatgcag ataagaaaaa tagacgtcgt cagaaagacc
    10201 agcattctca gaaagaggac aaggaacaac gtcattttgt cattcgtcag aagtgagtag
    10261 agaataagga gaagaaatga aaccctcaat tcatagtttg gcacatcaaa ccatgcagga
    10321 atgggtattg gagcagggag aaaagaaatt ccgtgcagat caaatctggg aatggctcta
    10381 ccgtaaacgt gtgcagtcat ttgaagaaat gaccaacctt tccaaggatt tgattgctaa
    10441 gctcaatgac cagtttgtgg tcaatccctt gaaacaaggt attgtgcaag agtctgctga
    10501 tggtacagtc aaatatctct ttgaattgcc tgacggtatg ttaattgaga ctgtactcat
    10561 gtgtcaacac tatggtttat ctgtctgtgt gaccactcag gtcggctgta atatcggttg
    10621 taccttctgt gcctctggtt tgattaagaa gcagcgtgac ctcaataacg gtgaaatcgt
    10681 agcgcaaatc atgctggttc agaaatactt tgatgagcgt ggtcaggatg aacgcatcag
    10741 ccatatcgtt gtcatgggaa tcggtgagcc ctttgataat tacaacaatg tcttgaattt
    10801 ctttcgtact atcaatgatg ataaaggaat ggctatcggt gctcgtcaca tcacggtttc
    10861 aatctcaggt ttggctcata aaattcgtga ctttgctgat gaaggggttc aggtcaatct
    10921 tgccgtgtct cttcacgcac ccaacaatga acttcgttca agtatcatga agattaaccg
    10981 tgcctttcc
//
LOCUS       DAVBCW010000062        10824 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVBCW010000062 DAVBCW010000000
VERSION     DAVBCW010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10824
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.062"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(<1..95)
                     /locus_tag="VQ514_001384"
     CDS             complement(<1..95)
                     /locus_tag="VQ514_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2500134.1"
                     /translation="MKLTYDDKVQIYELRKQGYSLEKLSNKFGIN"
     gene            154..336
                     /locus_tag="VQ514_001385"
     CDS             154..336
                     /locus_tag="VQ514_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HET2500135.1"
                     /translation="MKSQKIKVSFTQSKIVTPILLVNKTIVWYGAMPLLGKVDEMTIL
                     RLESASIVSELVAGLR"
     gene            636..1475
                     /locus_tag="VQ514_001386"
     CDS             636..1475
                     /locus_tag="VQ514_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HET2500136.1"
                     /translation="MIIKRILNHNAVIAQSKKDIYILLFGRGIAFGRKTGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITEKIINLGKITLGNNFDEIIYINLTDHIS
                     SSIERYKEGIIISNPLRWEISKYYKEEFELGKRALQIIKKELGIELPIDEAAFIALHF
                     VNANLENNFQESYKITEIIMGIEKIIQDFYCTEFNQDSIDYYRFITHIKLFAHRLVEN
                     TTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYNYLLTSSELVYLMAHIRRLTK
                     NLD"
     gene            1493..3331
                     /locus_tag="VQ514_001387"
     CDS             1493..3331
                     /locus_tag="VQ514_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HET2500137.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPDVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGAPVTPASYGSSVVPILIMV
                     WLMKYIEKIIAKLTPAITKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVKAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     NLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            3344..4759
                     /locus_tag="VQ514_001388"
     CDS             3344..4759
                     /locus_tag="VQ514_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HET2500138.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWIDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEN
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSAIPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDELNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
     gene            5351..6397
                     /gene="pheS"
                     /locus_tag="VQ514_001389"
     CDS             5351..6397
                     /gene="pheS"
                     /locus_tag="VQ514_001389"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HET2500139.1"
                     /translation="MSTIEEQLKALREETLASLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            6397..6906
                     /locus_tag="VQ514_001390"
     CDS             6397..6906
                     /locus_tag="VQ514_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2500140.1"
                     /translation="MIRKVEMADVEVLAKIAKQAFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            6983..9388
                     /locus_tag="VQ514_001391"
     CDS             6983..9388
                     /locus_tag="VQ514_001391"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HET2500141.1"
                     /translation="MLVSYKWLKELVGIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEILSCEDVPETHLHVCQVNVGEEEERQIVCGAPNVRAGIKVMVALPGARIADNY
                     KIKKGKIRGLESLGMICSLGELGISDSVVPKKFADGIQILPENAVPGEEVFSYLDLDD
                     EIIELSITPNRADALSMRGVAHEVAAIYDKAVNFKEFTLTETNEAATDALSVGIETDK
                     APYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDT
                     FEGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKS
                     SHVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGA
                     TVRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFT
                     VSVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTVTQKLRRQVRTIAE
                     GAGLTEIITYVLTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNV
                     ARKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAGVPVDFFYA
                     KGILEALFTRLGLEVTYTATAEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIP
                     ETYVAELNLSVIEAALQPAAPFVEITKFPAVSRDVALLLKAAVTHQEVIDAIQAAGVK
                     RLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEKKVNA
                     EVR"
     gene            complement(9561..10562)
                     /locus_tag="VQ514_001392"
     CDS             complement(9561..10562)
                     /locus_tag="VQ514_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET2500142.1"
                     /translation="MIKTFLSALSVILFVIPIITYSFFPSSNLNIWLSTRPILAQIYA
                     FPLATATMAAILSFVFFSLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIADDFNATMRHGALAKISSHRDAL
                     NVLPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            10718..>10824
                     /locus_tag="VQ514_001393"
     CDS             10718..>10824
                     /locus_tag="VQ514_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2500143.1"
                     /translation="MQDNYTTKGKHLTIDSRRLIERWKKEGKSNREIASL"
BASE COUNT         3466 a         1739 c         2290 g         3329 t
ORIGIN      
        1 ttgtttatcc caaatttatt tgaaagcttc tctaagctat atccttgttt tctaagttca
       61 tagatctgaa ctttatcatc ataagttagt ttcataataa aacaccccaa aagttagatt
      121 ttttctgtct aacttttggg gtgtagttca cagttgaaga gccagaaaat taaagtgtct
      181 tttactcaat caaaaatagt aacgcctatt cttttggtga ataagactat tgtttggtat
      241 ggtgcaatgc cattattagg gaaggtagat gagatgacca tattacgttt ggaatcagct
      301 agtatagttt ctgaactagt ggcaggttta cgatagagaa aatttttaaa aatttctatg
      361 tatgattttc atttctttag tgagactgtt gccattatca cattcgaatc acacaaaata
      421 aaaaaatttc tacaagtact tgacaaatag attgaaatat cataaaatga aaatggttac
      481 agagttatta attatttaag cttcatgtca ccattaaaaa ttgaaataaa aggatgttat
      541 cactaataca agtgagcagg aacctattta atcacatcgg aagaagtttc ttgatgtttt
      601 taagtaggtt ccttttattt taaaagggaa attttatgat cataaaacga atactaaacc
      661 acaatgccgt aattgcgcaa agtaaaaaag atatctatat tcttcttttt ggaaggggca
      721 tagcttttgg aagaaaaact ggagataaag taaatccaat tgatattgag aaaagttttt
      781 ttctcaaaaa tagagataat atgacccgtt ttacagagat gtttattaac gttcctttgg
      841 agttggtgta catcaccgaa aaaataatta acctaggtaa aataacattg ggtaataatt
      901 ttgatgaaat tatctatatt aatttaacgg atcatatttc ttcgagcata gaacgttata
      961 aagaagggat tattatttcg aatcccctac gctgggaaat atcgaaatat tataaagaag
     1021 aatttgaact tgggaaaagg gctttacaaa taataaaaaa agagttaggt attgaacttc
     1081 caattgacga agctgcattc atagcgctac attttgttaa tgctaattta gaaaataatt
     1141 ttcaagagtc gtataaaatc actgaaataa ttatgggaat tgagaaaatc attcaagatt
     1201 tctattgtac tgagtttaac caagattcta ttgattatta tagattcata actcatataa
     1261 aattatttgc tcatcgcttg gttgaaaata caacttattg tgacgatgat gatgaagact
     1321 tgttagcatt aatgaaaaat aaatatccta gagaatatga atgtggtgaa caggtggcaa
     1381 tgtttataca aactgagtac aattatttgc tgacttctag tgaattagtt tatttgatgg
     1441 ctcatattcg tcgcttaaca aaaaatttag attaatagaa taggagaata ccatgagtta
     1501 taaagatacg gtacaaaaaa tcctcgatgt aattggaggt gaaaaaaatg tcaatagagt
     1561 tacccattgt gtaacacgtt taagattaga attaaaagat gaaaatttag tcaatgatga
     1621 tgatgtgaag aagataccag atgtaatagg tattatgaaa aagaatggac aatatcaaat
     1681 tatacttggt aatgatgtag ctaattatta taaagaattc gttaaacttg gcaattttga
     1741 atccgattca gttgttcaag ggcacaaagg gaatatttta gaaagaatca ttgagtatat
     1801 cgctggttcc atgactccaa tcattccagc aatgttaggg ggaggtatgt tgaaagtctt
     1861 ggtaatcatt ttaccaatgc ttggtatatt gcaatcagat tctcagacta ttgctttttt
     1921 gacatttttt ggggatgctc catattattt cttaccgctg ttattagctt attctgcatc
     1981 acaaaaatta aaagtaacat ctacattagc tatgtctgta gcaggtgtac ttctccatcc
     2041 aaattttgtt caaatggtgc aatcagggaa tcctcttagt ttatttggtg cacctgtgac
     2101 accagctagt tatggttcat cagtcgttcc aattcttatt atggtttggt tgatgaaata
     2161 tattgaaaaa ataattgcta aattaacacc agctattact aagagttttt tgcaacctac
     2221 gctagtatta ttagtatcaa gctgtattgc cttagttgta gtcggaccta ttggagtaat
     2281 tgttggtgaa ggattatcaa atctagttgg gcaaatgtat ggtgtagctg gatggcttac
     2341 attagctatt cttggtgcta ttatgccatt tattgttatg actggaatgc attgggcttt
     2401 tgcacctatt tttttggcgg catctattgc tactccagac gtattaattc ttccagcaat
     2461 gttagggtca aacttagctc aaggggctgc ttcgatggct gttgcattaa agagtaaaaa
     2521 taataataca aaacaaattg cttttgcagc aggtttctca gccttacttg cagggattac
     2581 cgaacctgca ttatatggtg tgactttaaa atataaaaaa ccgctttatg cagctatgat
     2641 tggtggtgga ttagcgggat tatttgcagg tcttactagt gttaaagcat atctatttgc
     2701 tgtcccatct ttgatagcgt tgcctcaatt tatttattct gatgtgccat caaatattgt
     2761 aaatgcttta attgtggcgg tcatttcggt tgttattacc tttgtattag cttatatatt
     2821 tggaatcgat gaagaagaga gttctagcaa tttagaagtt aaagctggag tttcaaataa
     2881 aaaaatgata ttttctccta tatcaggaga aatcattccg ttaagcgatg tccaggataa
     2941 aacattttca gataaattaa ttggagacgg agtagcgatt atcccaagtg aaggtaaggt
     3001 ttatgcacca tttgatggga aaattacaaa tatttttccg actaagcacg caattggatt
     3061 gaagagtgat gagggtgttg agttactaat tcatattgga ttagatactg ttgagctaaa
     3121 aggtcaaggt tttattagtc atgtagaaga aggagacaga gttttcaaaa atcagttgat
     3181 ttttgaaatg aacttgaatt taatcaagac taaaggctac gaaacagtta caccagtaat
     3241 tgtaacgaat accaatgatt ttctagatgt attagtatta cctaataatc agacaatcga
     3301 gcattctaag gaattactgg taatattata atggaggaaa gttatgacca tttttccaga
     3361 tgattttctt tggggtggag cggttgcagc taatcaagta gagggagcat ataatgaaga
     3421 tggtaagggc ttatcagttc aggatgtgtt acccaaaggt ggattaggag aagcaacaga
     3481 aaatcctaca gaagataact taaaattgat aggtattgat ttttatcata aatataagga
     3541 agatatatcc ttgttttctg aaatgggctt taatgttttt cgtacttcta ttgcatggag
     3601 tagaattttt ccaaaaggag atgaggaaga acctaacgaa gctggtttga aatattatga
     3661 tgaattattt gatgaactac atgctcacgg gatagaacca cttgtaactc tttcacacta
     3721 tgagactcca ttatatttag caagaaaata tcatggatgg attgatagga gaatgattca
     3781 tttttatgaa aaatttgctc gaacagtttt agaaaggtat aaagataaag ttaaatattg
     3841 gcttacattt aacgaagtaa actctgtttt ggaattaccg tttactagtg gaggaataga
     3901 tatacctaag gagaatcttt cgaaacaaga attatatcaa gctatacatc atgaattagt
     3961 cgcctcgagt ttggttacaa aaattgctcg tgagattaat tcagagttta aggtggggtg
     4021 tatggtatta gctatgccag cttatccaat gactccaaat ccaaaagatg tatgggcgac
     4081 tcatgagtat gagaatctaa attatctatt ttcagatgtg catgttagag gttattatcc
     4141 gaattatgca aaaagatatt ttaaggaaaa tgacattaac atagagtttg cagctgaaga
     4201 tgcagagtta ttaaaaaatt atactgtaga ttttttatcc tttagctatt acatgagtgt
     4261 gactcaatct gctattccaa cacagtataa ttcaggagaa gggaatatta ttggtggttt
     4321 agtaaatcct tatttagagt cttcagagtg gggatggcaa attgatccaa ttggactacg
     4381 tataatttta aatagatatt atgaccgtta tcaaatccca ttatttatag tagaaaatgg
     4441 attaggtgct aaagatcaac taataaaaga tgaacttaat aacttaaccg tccaagatga
     4501 ttatagaatt cagtatatga aagaacattt attgcaagtt gctgaagctc tacaggatgg
     4561 tgttgaaatt atgggctata cgtcttgggg atgtattgac tgtgtttcta tgtccactgc
     4621 acaactttct aaaaggtacg gtctcattta tgttgatcga aatgatgatg gaaatggcac
     4681 attcaatcga tataagaaaa tgtcctttac ttggtataaa ggagtgattg aatcaaatgg
     4741 agaatcctta ttcaaatagt ttgtttattg atgattagat attatcacgg ttaaggcaac
     4801 atctcattaa atataaaata aagaggtttg gaagacatca aattctaaac ctctttattt
     4861 tataggggga ttaattaatt taattttgtt acagagagcc gattataaat agttaactta
     4921 ggatagattg gttatatata gtagactgaa tctaaaatag tacgaaacaa ttgctaaaac
     4981 atttatagaa attaatttta ctttcccaat cgatttgttt tcatcttatt tcaatccgct
     5041 ataaagttag ataagaataa gctaacaagc agaagtaaga taggtcttgc aagaatggta
     5101 aaaatctgat aaactaagta gtaattgaat aggaatctga aagactagta gcccaaggga
     5161 agtatatcag ggaggagagc cgtgactgca agctctctat atgaaagctg ggtgaattca
     5221 cttgcgcatg gatttagaaa tgaaggtctg acttgtcaga tgaaaacgga tggtaccgcg
     5281 tgtcaacgct ccgagtggag tttttggcat gtggttttct ttttatctac gagagactga
     5341 tggaggaaat atgtcaacta ttgaagaaca attaaaagcg cttcgcgaag aaacgctggc
     5401 tagcttgaag cagattactg ctggaaatga aaaagagatg caagatttgc gtgtctctgt
     5461 ccttggtaaa aagggttcgc tcactgaaat cctcaaaggg atgaaagatg tttctgctga
     5521 gatgcgtcca atcatcggga aacacgtcaa tgaagctcgt gatgtcttga ctgctgcttt
     5581 tgaagaaaca gctaagctct tggaagaaaa gaaagtcgcg gctcaactgg ctagcgagag
     5641 tatcgatgtg acgcttccag gtcgtccagt tgcgactggt caccgtcacg ttttgacaca
     5701 aaccagtgaa gaaatcgaag atatcttcat cggtatgggt tatcaagtcg tggatggttt
     5761 tgaagtggag caagactact ataactttga acgtatgaac cttccaaaag accacccagc
     5821 tcgtgatatg caggatactt tctatatcac tgaagaaatc ttgctccgta cccacacgtc
     5881 tccagttcag gcgcgtgcta tggatgccca tgatttttct aaaggtcctt tgaagatgat
     5941 ctcgccaggg cgtgtcttcc gtcgcgatac ggacgatgcg acccacagtc accaattcca
     6001 ccaaatcgaa ggcttggtag ttgggaaaaa tatctctatg gctgatcttc aaggaacgct
     6061 tcagttgatt gtccaaaaaa tgtttggtga agagcgtcaa attcgtttgc gtccatctta
     6121 cttcccattc acagagccat ctgttgaggt ggatgtttct tgcttcaagt gtggtggaga
     6181 aggctgtaat gtatgtaaga aaacaggttg gatcgaaatt atgggggccg gtatggttca
     6241 cccacgtgtc cttgaaatga gtggtatcga tgcgactgta tactctggct ttgcctttgg
     6301 tcttggacaa gagcgtgtag ctatgctccg ttatggaatc aacgatatcc gtggattcta
     6361 ccaaggagat gtccgcttct cagaacagtt taaataatga ttagaaaagt agaaatggca
     6421 gatgttgagg tgttggctaa aattgccaaa caagcctttc gtgaaacctt tgcgtatgat
     6481 aatacggaag agcagttaca ggaatacttt gaagaggctt atagtctgaa aactttgtca
     6541 actgagttgg gaaatcctga ctctgaaacc tatttcatta tgcatgagga ggagatagct
     6601 ggttttctca aagtcaactg gggaagtgct caaactgaga gagaattaga ggacgctttt
     6661 gaaattcaac gcctctatgt gctacaaaaa ttccaaggat ttggactagg taagcaactg
     6721 tttgaattcg cacttgaact tgctacaaaa aatagttttt cttgggcttg gctaggtgtt
     6781 tgggagcata atacaaaagc tcaagcgttt tataaccgat atggttttga aaaatttagc
     6841 caacatcatt ttatggttgg tcaaaaagta gatacggatt ggttactgag aaagaaatta
     6901 aggtaagaag agaattcttc tattgaaatg ataggaaagg aaaagaacca gtgtggcaat
     6961 ggtcattctg gagaactaaa ttatgcttgt atcttataaa tggttaaaag aattggtggg
     7021 cattgatgtg ccatcacagg agttggctga aaaaatgtca accacaggga tcgaggtcga
     7081 aggtgtcgaa tcaccagctg ccggtctttc aaaaattgtc gtcggtgaga tcttgtcttg
     7141 cgaagatgtg ccagaaactc accttcatgt ttgtcaggtt aacgttggcg aagaagaaga
     7201 gcgtcagatc gtttgtggtg ccccaaatgt gcgtgcaggc atcaaggtta tggtggctct
     7261 tccgggagct cgtatcgctg acaattacaa aatcaaaaaa ggaaaaatcc gtggcttaga
     7321 gtcacttgga atgatctgtt cacttggtga attgggtatt tctgactcag ttgtacctaa
     7381 gaaattcgca gatggtattc aaattttgcc agaaaatgcc gttccaggtg aggaagtctt
     7441 ttcatatcta gacttggatg atgaaatcat cgaactttct atcacaccaa accgtgcaga
     7501 tgccctttct atgcgtggag tggctcacga agtggcagcc atatatgaca aggcagtcaa
     7561 ctttaaagaa tttactttga cagaaacaaa tgaagctgcg acagatgccc tttctgtggg
     7621 cattgagaca gacaaggcgc cttactatgc agctcgtatc ttggacaatg tgactatcgc
     7681 accaagtcca caatggttgc aaaaccttct catgaacgaa ggcatccgtc ccatcaataa
     7741 cgttgtagac gtgacaaact acatcctgct ctactttggt caacctatgc atgcttttga
     7801 cttggacaca tttgaaggga ctgacatccg tgtgcgtgaa gcgcgtgctg gtgaaaaatt
     7861 ggtgaccttg gacggtgaag aacgtgactt ggacgtgaat gacctagtca tcactgttgc
     7921 agacaagcca gtagcccttg caggtgtcat gggtggtcaa gcaacagaaa tctctgaaaa
     7981 atctagtcat gttgtccttg aagctgctgt tttcaatggc aaatctatcc gtaagacaag
     8041 tggtcgcctg aaccttcgtt ctgagtcatc ttctcgcttt gaaaaaggaa ttaatgtggc
     8101 aacagttaat gaagcccttg atgcggcagc tagcctgatt gcggaacttg caggtgcgac
     8161 ggtgcgtaag ggtatcgttt cagcgggtga gcttgatact tcagatgtag aagtttcttc
     8221 aactcttgct gatgttaacc gtgtcctcgg aactgagctg tcttatgctg atgtcgaaga
     8281 cgtcttccgt cgtcttggct ttggtctttc tggaaatgca gacagcttta cagtcagcgt
     8341 accacgtcgt cgttgggata tcacaatcga agctgatctc tttgaagaaa tcgctcgtat
     8401 ctatggatat gaccgcttgc caaccagcct tccaaaagac gatggtacag ctggtgaatt
     8461 gactgtgaca caaaaactcc gccgtcaagt tcgtaccatt gctgaaggag caggtttgac
     8521 agaaatcatc acctatgttc taacaactcc tgaaaaagca gttgagttta cggctcaacc
     8581 aagtaacctt acggaactca tgtggccaat gactgtggat cgttcagtcc tccgtcaaaa
     8641 tatgatttca ggtatccttg ataccgttgc ctacaacgtg gctcgtaaga ataaaaactt
     8701 ggccctttac gagattggaa aagtctttga acaaacaggt aatccaaaag aagaacttcc
     8761 aaatgaaatc aacagttttg cctttgcctt gacaggcttg gttgctgaaa aagatttcca
     8821 aacagcagga gttccagttg atttcttcta tgctaaagga atcctagaag cgctctttac
     8881 tcgcttggga ctagaagtga cctatacagc aacagctgag attgccagcc ttcacccagg
     8941 acgtacagca gtcatttcac tcggtgacca agttcttggt tttcttggcc aagtgcatcc
     9001 agtcactgct aaggcttacg atattccaga aacgtatgtg gctgagctca acctttcagt
     9061 catcgaagct gcccttcaac ctgctgctcc atttgtggaa atcacgaaat tcccagcagt
     9121 cagccgtgac gttgcccttc tcctcaaggc agcagtaact caccaagaag ttatagatgc
     9181 tatccaagct gccggcgtga aacgtttgac agatatcaaa ctctttgacg ttttctcagg
     9241 tgagaaattg ggacttggta tgaagtcaat ggcttatagc ttgaccttcc aaaatccaga
     9301 agacagctta acggacgaag aagtcgcacg ctacatggaa aaaatccaag catcgcttga
     9361 aaaaaaagtg aacgcagaag tgcgttaaac gatagtagga tacaaattcc ccctctagtt
     9421 tatgaacact agaggggtga ttttatataa tactcaatga aaatcaaaaa gcaaactagg
     9481 aagctagccg taagctgtac ttgagtactg taaggcgagg ctgacgtggt ttgaagagat
     9541 tgtcgaagag tataaaataa ttaaaatgtg atttctgtaa aaatacatct atgatcagag
     9601 ttttgaaaac ttacaatgtc taaatcttta acatagtagt ggtttttagg caataaaata
     9661 tgatctattg ttgcattaaa aagttttgga ctttggctat tccaagttcc tctttcaaaa
     9721 ggtggtagta catttaatgc gtccctatga gagcttattt ttgcaagtgc tccatgacgc
     9781 atagttgcat taaaatcatc tgcaataata gcctttggat attttgaagc caattgatta
     9841 tgaatgatgt ttaagtcttg cttccagatt tccattaaac ctggcagagg aggagcagta
     9901 tgcaaggcaa tgatatctgg tatattttgt tttctcgaat gtaatacaat tgtcccgaac
     9961 cgtgttgtat gaaaagtttt agcttctgta tagaaaccat aacttttctt gacaatcaca
    10021 gtcacaggag ctattccact attggtaggt ggagaagtga aaatatcata gttggccata
    10081 gaaagtccaa cttgatgaaa caatagtttg attctctggt tttcttgctc acctctgata
    10141 ttggtagcta gttcagggaa tatagccata tcggcgtcaa aatggctaaa aattcgctca
    10201 atatgttgtg cttctatttg attagcgacg ttccaagtta ctaattttaa ggttttagtc
    10261 ttatttgatg cagaagaaag ggttttatct gttccgaata atagtaatat gagcgatagt
    10321 aagagcaaaa tgccagagta aaaccgtatt tgtttatttt tcttgtaaaa agatagggaa
    10381 aaaaatacga aacttaaaat agcagccata gttgcagtag ctaaggggaa ggcataaatc
    10441 tgtgccaaga taggtctggt agatagccaa atattaagat tagaagatgg aaaaaaagaa
    10501 taagttatga tagggataac aaagagaatg accgaaaggg cagagagaaa tgttttaatc
    10561 attgtgtgag tacctcaatt tgcaatggtt gctcggagct aatgtgactt tgtgacttta
    10621 cttgattgat attctttttc ttgtcttcct catagattaa gtacaaccca agtaaaaaaa
    10681 gtccaattta agattgaaat gattaaaagg cacagaaatg caagacaatt atactacaaa
    10741 aggcaaacat ttgacaatcg atagccgtcg cttaatcgaa agatggaaaa aagaagggaa
    10801 atcaaataga gaaattgcct ctct
//
LOCUS       DAVBCW010000063        10603 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVBCW010000063 DAVBCW010000000
VERSION     DAVBCW010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10603
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.063"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(6..1220)
                     /locus_tag="VQ514_001394"
     CDS             complement(6..1220)
                     /locus_tag="VQ514_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET2500144.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(1376..1669)
                     /locus_tag="VQ514_001395"
     CDS             complement(1376..1669)
                     /locus_tag="VQ514_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500145.1"
                     /translation="MMKNSFQKSNFLYYGIILVSVLVKVIMICQLESLVPLLYPSFIG
                     FLVFHVLYHLILFLVAKRSGRWDYLMIWGLFLMFNLLYDSFLGLLFLGLSFGM"
     gene            1831..2283
                     /locus_tag="VQ514_001396"
     CDS             1831..2283
                     /locus_tag="VQ514_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET2500146.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(2318..3706)
                     /locus_tag="VQ514_001397"
     CDS             complement(2318..3706)
                     /locus_tag="VQ514_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2500147.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     AASPSEEDFGQAVSQLHQALDKAAGKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            3772..4734
                     /locus_tag="VQ514_001398"
     CDS             3772..4734
                     /locus_tag="VQ514_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HET2500148.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            complement(4986..5996)
                     /gene="ccpA"
                     /locus_tag="VQ514_001399"
     CDS             complement(4986..5996)
                     /gene="ccpA"
                     /locus_tag="VQ514_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HET2500149.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKVGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(6421..7020)
                     /locus_tag="VQ514_001400"
     CDS             complement(6421..7020)
                     /locus_tag="VQ514_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2500150.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRSERLETKVVVVTTFKRAGYFERAVKAGVDAYVL
                     KERSIADLMQTLHTVLEGRKEYSPELMEVVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(7021..8118)
                     /locus_tag="VQ514_001401"
     CDS             complement(7021..8118)
                     /locus_tag="VQ514_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2500151.1"
                     /translation="MLERLKSIHYMFWASLIFMIFPILPVVTGWLSAWHLLIDILFVV
                     AYLGVLTTKSQRLSWLFWGIMLIYVAGNTAFVAVNYIWFFFFLSNLLSYHFSVRSLKS
                     LHVWTFLLAQVLVVGRLLIFQRIEVEFLVYLLGILTFVDLMTLGSVRIRLVEDLKEAQ
                     TKQNTQINLLLAENERNRIGQDLHDSLGHTFAMLSVKTDLALQLFQIQAYPQVEKELR
                     EIQQISKESMREVRTIVENLKSRTLTSELETVKKMLEIAGIEVEIANQLDTASLTQEL
                     ESTASMILLELVTNIIKHAKASKAYLKLERTDQELLLMVRDDGKGFETVTENELHTVR
                     ERVLPFSGEVSVISQKHPTEVQVRLPYKERK"
     gene            complement(8118..8855)
                     /locus_tag="VQ514_001402"
     CDS             complement(8118..8855)
                     /locus_tag="VQ514_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2500152.1"
                     /translation="MKRWIALNKIEFLLTKRQSVYYLLSVGMPTAFYLFFSGMYQDTP
                     GGPANFMRDYLISMTAFSMMSTAIFSFPAVLHTDKMNNWQKTLRHSPVNMVEYYLSKI
                     TSMMVDYLVSILVVFSVGHLVRGVDMSLGSWIGAAFLLIVGSIAFVALGLTLTLLPSS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQAIGKCLPTYQLMELLKTFLNEGSINL
                     SATVYLLVFSAVLFGLTIYLQGHKENA"
     gene            complement(8857..9741)
                     /locus_tag="VQ514_001403"
     CDS             complement(8857..9741)
                     /locus_tag="VQ514_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2500153.1"
                     /translation="MNMIKVQGLHKNIKSKAILKDISFEVAEGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVYIQDLPVTSSKLDYTRSYLPQENVIVQKLKVKELIAFFQRIY
                     PNPLNNQEIDQLLQFDQQQKEQFAEKLSGGQKRLLSFILTLIGRPKLVFLDEPTAAMD
                     TSTRQRFWEIVRDLKAQGVTILYSSHYIEEVEHTADRILVLNKGELIRDTTPLAMRSE
                     EIEKHFILPLAYKEVVEKSVLVENWVQKQDALQVVTCEADAFWELLVQAGCRIQEIEV
                     NNRSLLDTIFEETQKGDD"
     gene            complement(9728..9922)
                     /locus_tag="VQ514_001404"
     CDS             complement(9728..9922)
                     /locus_tag="VQ514_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500154.1"
                     /translation="MKKAILFLFIGLSLVVWLVEMFTGWFDQSFLHQVIRGAWGLGFM
                     ISVVFPMGMEWLKGEYYEHD"
     gene            complement(9919..10125)
                     /locus_tag="VQ514_001405"
     CDS             complement(9919..10125)
                     /locus_tag="VQ514_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500155.1"
                     /translation="MEEEEMRNKMIIAVSLVVAGVMTYLMFSGLDEGFYHFPWELFAS
                     FGIMSWLIREGLKLVRDVKKELEK"
BASE COUNT         3213 a         2411 c         1931 g         3048 t
ORIGIN      
        1 aagccctatc tacgatactg tttcaagaaa cgagtcatct tttcttggat ttgggctaac
       61 tcatcaacac gaggaaggta aacgatacgg aagtggtctg gttcctgcca gttaaagcct
      121 cgaccatgaa ccaagagaac cttttcctgc ttcaagaaat caaggacaaa ctgctcatca
      181 tcatcgatac ggtacatatt gcggtcgatt tttgggaaga tatagagccc cgccttgggt
      241 ttaacggcag acaaacctgg aatatcttga atggcattat agatgaaatt tctttgctcg
      301 tagattcgtc caccaggaag aagcaattca tcgactgatt ggtgaccccc caaggaagtt
      361 tgtacgactt gttgggccaa aacgttagag caaaggcgca tattggacag catattgagc
      421 ccttcgatat agcccttaac atgagtctta gggccagaca agaccatcca acccacacgg
      481 aaacctgcta tgcggtggga ttttgacaga ccattcatgc tgacacagaa gacatctggt
      541 gccaagctcg ccacaggcgt atgcacatgt ccgtccatta ccatgcggtc atagatttca
      601 tccgcaaaga taatcaaatc gttttgacgg gcaatctcaa taatctccag caagagttcc
      661 ttaggataaa gggctccagt tgggttattt ggattgataa ggacaattgc cttggtattg
      721 gaagtgattt ttgacttgat atcgtcaata tctgggtacc attccacagc ttcatcacag
      781 atatagtgaa cggcatttcc tccagctagg ctgacagccg ccgtccatag aggatagtct
      841 ggcattggca ctaagacctc atccccattg tccaaaagcc cctgcatgga catgacaatc
      901 aactcactga caccatttcc aaggtaaata tcatcaatat ctacgtttgg gaatttcttc
      961 agctggcaat actgcatgat ggccttacgg gctgagaaaa tccctttgga gtcagaatag
     1021 ccttcactat cacgcgcatt cataatcaag tcatgaatga cctcgtctgg cgctgtaaag
     1081 ccaaattctg ctggatttcc tgtattcaga cgtaaaatct tttctccatt cgctcgcatc
     1141 cgcatggctt cttccaatac aggaccacgg atatcataag caacatgctc taacttacta
     1201 gacttgttat attctttcat cttgtttcct caattcatca ggatagtaac attataccac
     1261 tagaaagaga atgcgacaag tttactgaaa tgtgttacta gtttcgtata gaagaaaaac
     1321 cttgctaaag gcgaggttaa gaacgtagag atagcaggca ttttgcaaag acacatcaca
     1381 taccaaaaga taaacctaga aaaagcaagc ctagaaagga gtcataaaga agattaaaca
     1441 tgaggaaaag cccccatatc atcaagtaat cccaacgccc acttcgtttt gcaactagaa
     1501 ataagattag atggtagagt acatgaaaga ctaaaaatcc aataaaactt ggataaagaa
     1561 gcggtaccag actctctaat tgacagatca tgatcacttt taccaagact gaaactagga
     1621 taattccata atacaggaaa tttgattttt gaaaagagtt tttcatcatt ctcccttcct
     1681 tcacttcttt ccttctatcc tttcttttat tttatcatat atttatatag attttaagcc
     1741 ctaatattct tttgaaaaat actttttttg ctttacttct atagtgaaaa ggtttacaat
     1801 tatagtaaga aaatttcagg aggtttcatt atggcacaac gttaccaaaa tatcatggtc
     1861 gcaatcgatg gttctaagga agcggacttg gcttttgtca agggagttca ttctgctcta
     1921 cgaaacgacg ctaaactcac catcgcacat gtcattgaca cacgcgctct ccaaagcgta
     1981 tccacctttg atgctgaagt ttacgaagaa ctccaagtcg acgctgaaag tctgatgaaa
     2041 gagtacgaaa aacgtgctaa agatgctggg gtagcagatg ttcatatcgt cattgaaatg
     2101 ggaaatccaa agaccctgct agcacgtact attccagatg ccgaggaagt ggacctcatc
     2161 ctcgttggcg caactggtct caacgccttt gaacgcctct tggtcggctc ttcatctgaa
     2221 tacatactcc gccatgctaa ggtcgatttg ctggttgtga gagaacaaga aaaaacctta
     2281 taatcacaaa gaaaaggagc ccctagctcc tttttgttta cgatttattt ctctctttat
     2341 ggcgttcgta agccttgagc tggcgctgca gttccttttt aatagcaggt tctggagcat
     2401 atttttcttc ccaattatct ggttttaaga ttttatgggt cactggatca aaatgagcct
     2461 tgccatctgg aaaaattttc cccatattgg cctgatggac aatatcaaaa atacgttctg
     2521 ggtccacccc catcaagaca aaactgccgt aggtgaagta aagcgtgtca atcaaggcat
     2581 ccacttgccc tatcaaatct tgctgagcag gtgtcttctt ggctacttta cctgctgcct
     2641 tatcaagggc ctgatgaagt tgcgatacag cttgaccaaa atcttcttca gaaggactgg
     2701 ctgctcgaac aaactccacc aattcttcta ttttaaaacc agccctatgg gttgcaccct
     2761 ctaaatccca agctcgaggt tcttcttggg ttcgttcatc catcatgtgg tggaaggtct
     2821 tgaccttatt gaaatgatag tcacggctga caaagacttt ttctgaagac aaaacaccaa
     2881 aatgttccaa ggccttgtgg atcccatctt gttgattgct agtggtaatg tgcttggcag
     2941 cttccttgac actgctacta ccatttccca tagcgaccga cataccgacg ccagccagca
     3001 tttcaaggtc attatctgag tcaccaaaag ccatgacttg gttgaggtca aagccatatt
     3061 ctttcccaac tcggcgaatg ccttctaatt tagaatttcc ctgattgatg acatccgatg
     3121 caaaaggatt gctacgtgtc aatttcaagt cttcaaaatc agctgccgcc ttctcagatt
     3181 cttctggtgt catcagcatc aaaacttggt agataggctg attcatcagg tgaagcaggt
     3241 cctcttcctt ttggggaaca accttgctga ccatgcgatt aaaagactga ctcaccgtcc
     3301 gagttaaaac agagggaacg aagcgactaa ttcgttggga aaaagaaccc agaccaaagg
     3361 acatgatttt agaacccaac atggcatcct tggtccctag ggcaatctcc gtgccctctt
     3421 ttttagcata gctaattaga tggcgcaaat gtaacttgga aatagggctc gtgaacaaga
     3481 ctctgtcttt actaaagata tactggccat tataggttac cgcaaaatcc agatccaaat
     3541 cgtccatcaa ttccttaaca aaaaaaggtc ctcgccctgt cgctacgcca actagtaccc
     3601 cttgttcttt gacaatctta atcgcgtcct tagtggattt caaaacactc ttgcgattgt
     3661 tgaccaaggt tccatcgata tcaaaaaaaa cagctttgac ttccatccta ttccaatctc
     3721 cccttttgtg atacaatgat tataccacat ttcagaaaga gtgagtaaat catgcctaag
     3781 aaaatccttg ttttacatac gggtggaact atttccatgc aggccgatgc ttctggcgct
     3841 gttgtgacga gttcagataa tcccatgaac catgtgtcca acccacttga aggaatccaa
     3901 gtccacgcct tggacttttt taaccttcca agtccccata tcaaacccaa acatatgctg
     3961 gtcctctacc agaaaattaa agaggaagca gataactacg atggagtggt aatcacacac
     4021 ggaaccgata ctttagagga aacagcctat ttccttgata ccatggaagt tccccatatg
     4081 cctatcgttc taacaggagc catgcgtagc tccaatgagc tcggtagtga tggtgtttat
     4141 aattacctaa gtgctttacg agtggccagc gatgacaggg ctgctgacaa aggagttttg
     4201 gtcgttatga acgatgaaat ccacgctgcc aagtatgtca ccaaaacaca tacgactaat
     4261 gtcagcacct tccagactcc aacacatggc cccctcggtc tcatcatgaa acaggaaatc
     4321 ctctacttca aaacagctga acctcgtgtt cgctttgacc ttgatcacat acaaggttta
     4381 gtccctatca tctcggctta tgctggtatg acagatgagc tgattgatat gctggattta
     4441 gaacacttgg acggtttgat tatccaagcc ttcggagctg gtaatattcc caaagaaacg
     4501 gctcaaaaat tagaaagcct tctgcaaaaa ggaatcccag tcgctctggt atcacgatgc
     4561 tttaacggta ttgccgagcc tgtttatgcc taccagggtg ggggcgtaca gttgcaaaaa
     4621 gcaggcgttt tctttgttaa agaactcaac gcccaaaaag ctcgcttgaa actcctcatc
     4681 gccctcaatg ccggactaac aggacaggct ttgaaagact atatggaagg ctaatactct
     4741 tcgaaaatct ctgcaaacca cgtcagagtc gccctaccgt atgtatggtt actgacttcg
     4801 tcagttctat ctacaacctc aaaaacatgt tttgagctga cttcgtcagt ttcatctaca
     4861 acctcaaaac agtgtgttgc gcagccttta accagccgcc tagtccgctc tatggtattc
     4921 attaagtcaa catctcttgt ttaagagcac aaaatcagga aatcttctcg attccctgat
     4981 tttttctatt tacgttttcg tgttgagcta cgttctgtca aaccatgagg taagagaact
     5041 tcacgttctt ccaactcttc cttatgcata atcttggtca acatacgcat actaatggca
     5101 ccaaggtcat aaagaggttg ggcaatcgtt gtcaagtttg gacgggtaaa gcgtgagatt
     5161 tgtgaatcat cactagtaat aatttcaaaa tcttctggca cagaaacacc cttatcagcc
     5221 aaaccgttca agactcctgc tgccaactca tcacctgtca caactgctgc agttgcattt
     5281 gatgaaatca aacgctctgc taaggcgtaa ccatcatcat agctatattt agattcaaat
     5341 accaaaccct cactataagt gattcctact tttttcaagg tttccttgta gccaactaaa
     5401 cgaaccttac cattgatgtc atccactagc ggaccgctaa cgaaagcaat acgctcattt
     5461 tctttagcaa ggtagctcac tgcatcaatt gttgcttgct tatagtcaat attgacactt
     5521 ggcaactggt gctcaacatc gacagttcct gcgagaacaa tcggagtacg tgaacgcgaa
     5581 aattctgagc gaattttatc tgtcaagtga taacccatat agataatgcc atctacctgc
     5641 tttgaaaaga gggtattgac aacagaaact tctttctcgt tatcttcatc gctattagct
     5701 aggacaatat tgtacttgta catttctgca atatcatcaa tccccttagc caaactcgaa
     5761 aaataaccat tggtaatatt tggaatcacg acaccgacag tggttgtctt tttacttgca
     5821 agaccacgcg caactgcatt tggacgataa tccaaacgat caattacctc tagcactttt
     5881 ttacgggtat tctcttttac atttttattg ccattgacca cacggctgac cgtcgccatg
     5941 gaaacacctg cttcacgagc gacatcataa atggttactg tatcatctgc attcattcct
     6001 tttcctgtcc tttctatctc acacattctt ttacaagtag aggtactgat tgaagctcta
     6061 tatctactta caaaagtgaa gatgtgaaaa tttcgttttc atatttctac ttattccatt
     6121 ctatcactaa ttgtaaacac tttcaagtgt tttttgaaga ttgattgaaa aaatttcata
     6181 gaaaacctag gtttagctcc ttgctaccac cttagactaa ataaaaagga ggaaactaag
     6241 cccttctaaa gttatagtaa aatgaaataa gaacaggata aatcgatcag gacagtcaaa
     6301 tcgatttcta acaatgtttt agaagtagag gtgtactatt ctagtttcaa tctactatag
     6361 gtattgttcc attcactacc gtcaatttta gcacatagtc ttcatgaaaa tattatatca
     6421 tcataaccaa ccagattctt ttgcgatatt agctgcctct gttcgattac cagcatccag
     6481 ttttgaaaga atattggtga catagtttcg aatagttccg tttgagaggt aaagctgatc
     6541 tgcgatttct tggttggata agccccgggc gattcccttt aagacagcaa tctcttgttc
     6601 tgtcaacgga ttgggacggg tcatcaccac ttccatcaat tcaggcgaat actccttgcg
     6661 tccttcgagg acggtgtgca aggtttgcat gaggtctgca atgcttcgtt cctttaatac
     6721 ataagcatcc actccagcct tgaccgcacg ttcaaaatac ccagcacgct tgaaggtcgt
     6781 caccacaacc acctttgttt caagcctttc tgatcgtatc cactccaaga cttcaagacc
     6841 tgtcttaaca ggcatttcta cgtcaaggat ggcgatatct acagactcct tttctaatag
     6901 ttggattgct tcttgcccat tcttggcttg aaggacagac tccacatctg gttgaagcgt
     6961 gagcaattga cacatagcat ctcgcaacat actttgatct tctgcgacta atactttcat
     7021 ctactttctc tccttataag gtagtcgaac ttgcacttcc gttggatgtt tctgactgat
     7081 tacacttact tctcctgaaa atggaaggac acgttctcga actgtatgga gctcattttc
     7141 tgttacagtt tcaaaacctt tcccgtcatc tctgaccatc aggagcaatt cctgatctgt
     7201 gcgttctaat tttaagtagg ctttagacgc tttagcatgt ttgatgatgt tggtcactaa
     7261 ttcaagcaaa atcatggaag ccgttgactc taattcctga gttaagctag ctgtatctag
     7321 ttgattagct atttccacct caattccagc aatttctaac atctttttca cagtctctag
     7381 ttcggatgtc aaagttctag acttaagatt ttccacaatg gttcgcactt cacgcattga
     7441 ttctttgcta atctgctgta tttctcttaa ttccttttcc acttgtggat aagcctgtat
     7501 ttgaaataac tgcaaggcta gatcagtttt aacactaagc atagcaaagg tatgccccag
     7561 actatcatgc aaatcttgac cgatacgatt gcgctcattt tctgctagca atagatttat
     7621 ctgggtattt tgcttagtct gagcttcttt caaatcctcg acaaggcgaa ttcgaaccga
     7681 gcccaaagtc atcaaatcaa caaaagtaag aattccaagc agatagacta gaaactcaac
     7741 ttcgattctc tgaaaaatca aaagtcgccc cacaacaagg acttgagcaa gaagaaaagt
     7801 ccagacatgt aaagacttta aactacgtac gctgaaatga taacttaaga gattggaaag
     7861 gaaaaagaaa aaccagatat aattcacagc aacaaaggcg gtattcccag ctacatagat
     7921 cagcatgatc ccccagaaaa gccaagatag gcgctggctc ttagttgtta aaacacccaa
     7981 atatgccact acaaatagaa tatcaatcaa taaatgccag gcagaaagcc acccagtcac
     8041 tacaggtagg atggggaaaa tcataaaaat taaactggcc caaaacatat agtgtatgct
     8101 tttcagtctt tcaagcatta agcattctcc ttatgacctt gaaggtaaat ggtcaaacca
     8161 aacaaaactg ctgaaaaaac aagtagataa actgtggctg atagattgat gctaccctcg
     8221 tttaagaagg tcttgagcaa ctccatcaac tgataagttg gtaggcactt cccgattgct
     8281 tgcatccagt ctggaaataa agagatgggc atccagagtc cacctaaaac agccaagcct
     8341 agatagagaa gattgcccac gacagacatc agctgactag aaggcaagag tgtcaaggtc
     8401 aagccaagcg ctacaaaagc tatacttcct actatcagca agaacgcagc cccaatccag
     8461 cttcctaaag acatatccac acctctgacc aagtgcccaa ctgagaaaac aaccaggatt
     8521 gagaccaaat aatcaaccat catacttgtt atctttgata gataatattc taccatattt
     8581 acagggctat gacgcaatgt tttctgccag ttgttcatct tatcggtatg taagacagct
     8641 gggaatgaaa agatagcggt cgacatcatg gaaaaggcag tcatggagat gaggtagtcg
     8701 cgcataaaat tagccggtcc acctggtgtg tcctggtaca tgcctgaaaa gaatagatag
     8761 aaggctgtcg gcatccctac agataataaa taatagactg attgccgttt ggtcaataga
     8821 aattctatct tgtttagtgc aatccatcgt ttcatcttag tcatctccct tctgtgtttc
     8881 ttcaaagatt gtatccaaca aactacgatt attgacttcg atttcttgta tcctacatcc
     8941 tgcttgaact aacagttccc agaaagcatc cgcttcgcat gtgactactt gtaaggcatc
     9001 ttgtttttgt acccagtttt caaccaagac tgacttctca acgacttcct tgtaagctag
     9061 cggaaggata aaatgctttt caatctcctc actacgcata gctagaggcg tcgtgtcacg
     9121 aatcaactct cccttattta aaaccaaaat ccggtcagct gtatgctcta cctcttcaat
     9181 ataatgagac gaatagagaa tggtgactcc ttgcgctttt aggtcccgaa caatttccca
     9241 aaaacgttga cgagttgaag tatccatggc agcagttggc tcatctaaaa agacaagctt
     9301 tggtcgccca attaaggtca agataaaaga taaaagacgc ttttgcccac ctgacaattt
     9361 ttctgcgaat tgctctttct gttgctggtc aaactgtaat agttgatcga tttcctggtt
     9421 gttcaaggga tttggataga tacgttgaaa gaaagcaatc aactctttaa cctttagttt
     9481 ttgaacaatt acattttctt gagggagata ggatctcgta tagtctaact tagagctcgt
     9541 taccggcaaa tcttggatat atacttgccc actggtgacc aatttatctc caagcaaaca
     9601 gtccaagagt gtcgtcttac cagctccatt tggcccaatc aaggcgacgc attcaccttc
     9661 agctacctca aaggaaatat ccttcaaaat agccttactt ttgatgtttt tatgtaagcc
     9721 ttgtacctta atcatgttca taatattctc ctttcaacca ctccattccc atagggaaaa
     9781 cgacggaaat cataaatcct aatccccagg cgccgcgaat aacttggtga agaaaggatt
     9841 gatcaaacca acctgtaaac atttctacca accataccac tagtgatagt ccaataaaaa
     9901 gaaagagaat cgcttttttc atttttcaag ctcctttttc acatctctta ctaatttcaa
     9961 gccttctcgt atcaaccaag acatgattcc aaagcttgca aacaactccc aaggaaaatg
    10021 atagaatcct tcgtccaatc ctgagaacat gagataagtc atgactcctg ctactactaa
    10081 actcactgcg ataatcattt tatttctcat ctcttcttcc tccatttcat actacaatta
    10141 tagtcttttg aaatcagagg agatagaagc ttctgtcact aaaaaatatg acaaatgtca
    10201 taaaaaattc tgttcaaaac aagcaagata cactatacaa taaaacacaa ttagataaat
    10261 ctaaggcaac ttcctcaaaa gagatatcaa accccattca cactataatg taaactaata
    10321 cttatttaaa gtcaaaagag cggaaatttt tatcgtacaa acatatatat tataggtaag
    10381 acaaaaaact cttcaattac tttcttaaaa atttatagga gttataaaat ctattaaatt
    10441 tcaatgtgga tacctaaact acagtattta ttgaaaagac tggagacaaa aagtatacgc
    10501 tgccaaaatg aattgctgaa aatcaaaaaa gagagaagca aactgattct ctcttaatgt
    10561 atataatatc tagttataaa aatacacact cacatatctc tgt
//
LOCUS       DAVBCW010000064        10542 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVBCW010000064 DAVBCW010000000
VERSION     DAVBCW010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10542
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.064"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            51..299
                     /locus_tag="VQ514_001406"
     CDS             51..299
                     /locus_tag="VQ514_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500156.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRIYSGNLILQELITVNGAYKATYIGELSSN"
     gene            430..1602
                     /locus_tag="VQ514_001407"
     CDS             430..1602
                     /locus_tag="VQ514_001407"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HET2500157.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENADSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            1602..2876
                     /locus_tag="VQ514_001408"
     CDS             1602..2876
                     /locus_tag="VQ514_001408"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HET2500158.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLEFANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLIGILSNYATDSL
                     VTASCRMAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VAFSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(2912..3877)
                     /locus_tag="VQ514_001409"
     CDS             complement(2912..3877)
                     /locus_tag="VQ514_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2500159.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQTIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(3858..5312)
                     /locus_tag="VQ514_001410"
     CDS             complement(3858..5312)
                     /locus_tag="VQ514_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HET2500160.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKSQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEHPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            5689..7572
                     /locus_tag="VQ514_001411"
     CDS             5689..7572
                     /locus_tag="VQ514_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HET2500161.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYALADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFEAKV
                     AQGNKVKAGDVLGTFDSNKIAAAGLDDTTMVIVTNTADYASVAPVATGSVSKGDAVIE
                     VKI"
     gene            7721..8608
                     /locus_tag="VQ514_001412"
     CDS             7721..8608
                     /locus_tag="VQ514_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2500162.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEMVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGTSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(8928..9545)
                     /locus_tag="VQ514_001413"
     CDS             complement(8928..9545)
                     /locus_tag="VQ514_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HET2500163.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(9960..10148)
                     /locus_tag="VQ514_001414"
                     /pseudo
     CDS             complement(9960..10148)
                     /locus_tag="VQ514_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         3062 a         2061 c         2337 g         3082 t
ORIGIN      
        1 tactttcctt attgtctgta aatctactga ccttgttgtt tatcccagtc atggtttcta
       61 gttcgggctc agagtttcaa agtggatggc aagagcatca attgattgct gagaaggtta
      121 gtaaaacact tgataagaca tttgataagg atgtcagaga aattccgacc agtcagtttt
      181 atcaaaaatt tgtagatgag atgggaagga tttactcagg aaatttgatc ctccaggagc
      241 tgataactgt gaatggagct tataaagcta cttatatcgg tgaactctct agcaactaat
      301 cattctttaa aatgatgacc aataagaggc ttgatttggt gaatcaagtc ttttttctct
      361 cttttactgc catttgtgat aaaatagtat agaacgattt tttacatgaa tgataaaaca
      421 gaggtaaata tgacaatcgg tattgataag attggttttg cgaccagtca atatgtcttg
      481 aaattacaag acttagcaga agcgagggga attgaccctg aaaaattaag taaaggactc
      541 ttactcaagg aattgagtat tgcgccccta actgaggaca tcgtgacctt ggcggccagt
      601 gctagtgact ctattttaac tgagcaagaa agacaagaag ttgacatggt cattgtggct
      661 accgagtcag gaattgacca gagtaaggct gcggccgtct ttgtgcatgg cttgctgggc
      721 atccagccct ttgctcgtag tttcgagatt aaagaagcct gctatggggc gacagctgcc
      781 ctccattatg ccaaattgca tgtggaaaat tctccggagt ccaaggtctt ggtcattgcc
      841 agtgatattg ccaaatatgg tattgaaact ccgggagaac caactcaagg tgctggaagt
      901 gtagctatgt tgattacaca aaatccacgc atgatggcct ttaataatga caatgtagct
      961 cagacccgtg acatcatgga tttctggcga ccaaattact cgacaactcc ttatgtaaat
     1021 ggtgtctatt ctacccaaca atacttggat agtttgaaaa cgacttggct tgaatatcaa
     1081 aaacgctacc agcttactct ggatgatttt gcggctgttt gtttccactt gccttatcct
     1141 aaattagcgc taaaaggctt gaaaaaaatc atggataaga gcctgcctca agagaaaaaa
     1201 gacctcttac aaaagcattt tgaccagtct attctctaca gtcaaaaggt ggggaatatc
     1261 tacacaggtt cacttttcct tggacttttg tctctcttgg aaaatgcaga tagcttgaaa
     1321 gctggggata aaatcgccct ttatagttac ggaagtggag ctgtggctga gttcttcagt
     1381 ggtgaattgg ttgaaggata tgaagcttat ttggataaag accgcttgaa caagctcaac
     1441 caacgaactg ccttatccgt tgcagactat gaaaaggtct tttttgagga agtaaacttg
     1501 gatgaaacaa actctgccca gtttgctggc tatgaaaatc aagattttgc cttggttgaa
     1561 attctcgacc accaacgccg ttatagcaag gttgaaaaat aatgaagata agttggaatg
     1621 gattttctaa aaaatcatac caagagcgcc tcgagctgtt aaaagctcag gcgctcctta
     1681 gtcctgagag acaagctagt ctggagaagg atgaacagat gagtgtgact gtggcagacc
     1741 agctgagtga gaatgtagtg ggaacttttt ctctgcctta ttcgctggtt ccggaggtac
     1801 ttgtcaacgg tcaggaatac accgttccct atgtgacaga agaaccctct gtggttgcgg
     1861 cggccagcta tgccagcaaa atcatcaagc gtgcaggtgg ttttactgca caagtccatc
     1921 agcgccagat gattgggcag gtagcccttt atcaagttgc taatcctaaa ctagcgcaag
     1981 agaagattgc cagcaagaaa gcggagctct tggagtttgc caatcaagcc tatccttcta
     2041 tcgttaaacg tggaggtggg gcgcgtgatc tgcatgtcga gcagataaaa ggcgaaccag
     2101 actttctcgt tgtttatatt catgtcgata cccaggaagc catgggtgcc aatatgctca
     2161 acaccatgct ggaagccttg aaaccagtct tagaagaact cagtcaggga cagagtctca
     2221 taggaatcct gtccaactac gcgaccgatt ctctggtgac tgcaagctgt cgcatggcct
     2281 ttcgctactt gagccgccaa aaggatcaag gacgagagat tgcggagaaa attgcgttgg
     2341 ctagtcagtt tgcgcaggct gatccttacc gagctgctac tcataataaa ggaattttta
     2401 atggtattga tgcgattttg attgccactg gtaatgactg gcgtgccatc gaagctgggg
     2461 cccatgcctt tgccagtcga gatggacgct atcaaggtct tagttgctgg acgctggacc
     2521 ttgaaagaga agaattggtc ggtgagatga ccctgcccat gcctgtagcg actaagggtg
     2581 gctctatcgg cctcaaccca cgtgtagctt tcagtcatga tctactagga aatccttctg
     2641 ccagagaatt agcccagatt atcgtgtcca tcggtcttgc ccaaaatttt gcagccctca
     2701 aagctttggt aagtacgggc atccagcaag gccacatgaa actacaggcc aaatccctag
     2761 ctctcctagc tggggctagt gaatctgaag ttgctcccct agtagagcgc ctcatctcag
     2821 ataaaacctt taacctagag acagcccagc gctatctcga aaatttaaga tcataaaaaa
     2881 ctcagacgaa tcggtctgag ttttcttgtg tttaaatact ttttcctggt aatagtgtaa
     2941 ctggtaagaa gtaaccagtt gtggcaactt ccttgccttc aatcttttgc aagagaagat
     3001 caatagtgag acaagcaatc tcttccaaag gttgcttgat agtagccaat tgagggtagt
     3061 aattttcgat aaagtaggtc ccatcatagc cgatgacctt gagctctttt gggacagaaa
     3121 tgcccaattc ttgagcgatt ttaatgacca gaatagctgt caaatcatcc gaagcaaaaa
     3181 tggcatctgg tttttcccgg gtcaagatat tcttgatttc catttctttt ctgacgggag
     3241 aaaagtcact ggaaacattg ataataggag cttttgggag tacggatgca aaaccagcgt
     3301 ggcgtagtcc ggttggcgaa ttggaattgt cattccctgt aatcatgatg atagtctggg
     3361 cacctgtctt gaccaaggtt tgggcagcaa gaaccccacc agcatagttg tcagaggaga
     3421 cgacagggat gtctggcgat aggtttcggt caaaggaaat aatcggcgct gtcacacgat
     3481 tgtagtcttc gattcctagg ttgtgactac cagaaatgat gccgtccacc tgattggctt
     3541 ccaacatttc gatgtattcg cgttccttct cagaatcatg ttcactgttg cagatgatgg
     3601 tcttgtaacc atttttgaag agttggtgtt ccaatttatc aatcaattct gcatagaaaa
     3661 cattggaaat attagggaaa atcaagccga ttaacttagc tgattttcct tgcagactac
     3721 gagccaggtt gttgggttta tagcccaatt ctcgcatggc ttcattgact ttttggatgg
     3781 ttttctcaga tagataccct tttttattga taacccgaga aacggtagta ggactgacgc
     3841 ctgcaagttt ggcgacatca gttagttttg cgaccataat ctaattcata gtaagttcca
     3901 gttgggtttc cagatttgat caggatacca ttttggtccg catgtgggaa gacacgacca
     3961 gaaaatactt tttctccttt attgatgaaa atttcaaaga cagagttatc gatgaagatt
     4021 gtagcagtag tagcctgatt ctcgataggg caagaacgag ttgtcccaaa ttcttgggca
     4081 tactgttctc cagcctggct acgatccact gtcacttgac cgtttacaag gtcaaagttg
     4141 attgaaagtc ccttaccttc tttatcagca agtaagacaa tctcgctctg gctattagct
     4201 tccaagttga gttcaagttc gtaagtgttc ttggtttggg aacggtttga gaaggcttct
     4261 tcagaagcac gaaggtcctt aatagcagcg actggatact ggtagagctt gtcgtctttg
     4321 atagtgagtt ccttgaccaa agagaaggtt ccttggtggt caaaacggtc agatgggtaa
     4381 gaaacatctg gcaaaccaag ccagctaact gctagagcac gcccatcagg agcgttgaag
     4441 gcttgagttg cataggcttc gaaaccgtaa tccatgtttt gaagttgaga cacatctacc
     4501 attttggcat ttttagggtc aaaggaagcc ccaatcttat acatatttgg aaagatatta
     4561 tcgtagtcta gaactttctt atccaatcct tgtggacagt agagaaggac aggatgttcc
     4621 tctacaaaga ccagattagg acattccatc atgtaggcag tacggtcgtt agcaaagtca
     4681 aggtcgccaa ctgcttgcca gtttgtgtag tcgttattga cagccttgta gagacggacg
     4741 aagccttttt tctccaagtc ctgtccaccg acgacagcat agtattgact tttaaagtta
     4801 aaaatttgtg gatcgcggaa gtggtcagta gagtctgctg gctggtcaat caagatcttg
     4861 tcaatctttg taatcttacc ctccttgtcc atcaaagcac cgatctggta tgggtgacgg
     4921 atccagtttt catcgcgaac atttcctgta taaaatagga ataagttatc gccaaattgc
     4981 atggcagaac cagagtaggc accgtggcta tctaatggag tatctggtaa aactttgatt
     5041 ccagtttctt taaagtgaac caaatcatca ctttctagct gtgcccaaga tttcaaaccg
     5101 tgggctgcac caaaaggaaa attctggtaa aagaggatcc acttgccatc aaagtaagaa
     5161 aagccatttg ggtcgttgag aagtcctgtt tttggctcaa catggtaatg agtatgccat
     5221 ggagattgtg ccatattttc ctttatatgt tgaatttctt cttgcgtcca atcttgataa
     5281 agtctgtaac gacgctcagt tgtccattcc attcattcct ccaaaaatct tatcactttt
     5341 aatagtaact gttttcgata aaaaatgcaa gccttttatg ttaaaaaaga ggaaaaactg
     5401 tcaaacgatt atcataaaat aaatacagga aatcaatcaa atttttacga aaatgtgaaa
     5461 gataatgaaa taaaagactt tttaaccaag gtttcaaact tatttttcaa ggaattcctg
     5521 atgaaacagt caaaaacaat caatctaaaa cttgcccact aagtggatag aatatttttt
     5581 ctgcaaaacg cttgacatat ttttaaaaca tgataaaata atagtcgtaa gggcgaataa
     5641 aatcgctttc aaacaagaac aaaatgttat ataaggagat ttttgcaaat gaacaatcag
     5701 gaaattgcaa aaaaagtcat cgatgccttg ggcggacgtg aaaatgtcaa tagtgttgcc
     5761 cactgtgcga ctcgtctacg tgtcatggtc aaagatgaag agaaaatcaa taaagaagtg
     5821 attgagaact tggaaaaagt tcaaggtgct ttctttaact cagggcaata ccaaattatc
     5881 tttggtacag gtacagttaa caaaatgtac gatgaagttg ttgtacttgg cttaccaaca
     5941 tcatctaagg atgacatgaa agcagaagtt gctaaacaag ggaactggtt ccaacgtgct
     6001 atccgtactt tcggtgatgt tttcgttcca atcatcccag ttatcgtagc gacaggtctc
     6061 ttcatgggtg tgcgtggtct tttcaacgct cttgaaatgc cacttccagg tgactttgca
     6121 acttacacac aaatcttgac agatacagcc ttcatcatct tgccaggttt ggttgtgtgg
     6181 tcaaccttcc gtgtatttgg tggaaatcct gccgttggta tcgttcttgg tatgatgctt
     6241 gtctctggct cacttccaaa cgcttgggca gttgctcaag gtggtgaagt aacagcgatg
     6301 aacttctttg gtttcatccc tgttgttggt ttgcaaggtt ccgttcttcc agccttcatc
     6361 atcggggttg tcggagctaa atttgaaaaa gctgtccgca aggttgttcc agatgtcatt
     6421 gacctcttgg taacaccatt cgtgacactt ttggtcatgt ctatccttgg actctttgtc
     6481 attggaccag ttttccacgt tgttgaaaac tacatcctta ttgctacaaa agcgattctt
     6541 agcatgccat ttggtcttgg tggtttcttg attggtgggg ttcaccaatt gatcgtcgtg
     6601 tcaggtgtgc accacatctt caacttgctt gaagtgcaat tacttgctgc tgaccatgct
     6661 aacccattca acgctatcat cacagctgct atgacagctc aaggtgctgc tactgttgcg
     6721 gttggtgtta aaacaaaaaa tccaaaactg aaaacacttg ctttcccggc tgctctttct
     6781 gccttcctag gtattacaga gcctgctatc ttcggggtga acttgcgctt ccgtaaacca
     6841 ttcttccttt cattgattgc tggtgcaatc ggtggtggat tggcttctat ccttggactt
     6901 gctggtactg gtaatggtat caccatcatc cctggtacaa tgctttatgt tggtaacgga
     6961 caacttccac aataccttct tatggtagct gtatcatttg cccttggttt tgctcttact
     7021 tacatgtttg gttacgaaga tgaagtagac gcaactgcag ctgcaaaaca agctgaagtg
     7081 gctgaagaaa aagaagaagt tgcgccagca gctcttcaaa atgaaacact tgtaactcct
     7141 atcgtcggtg atgttgtcgc tcttgctgat gtcaatgacc cagtcttctc aagtggagct
     7201 atgggacaag gtatcgctgt gaaaccaagt caaggcgtgg tctatgcact agctgatgct
     7261 gaagtttcaa ttgcctttcc aacagggcac gcttttggtt tgaaaacaag aaatggtgct
     7321 gaagttttga ttcacgttgg tattgacact gtatctatga acggtgacgg ttttgaagca
     7381 aaagttgctc aaggtaataa ggtgaaagct ggcgatgttc ttggaacatt tgactcaaac
     7441 aaaatcgctg cagctggact tgatgataca acaatggtta tcgttacaaa tacagctgac
     7501 tacgcttcag tagctccagt cgcaacaggt tcagtttcga agggggatgc tgtgatcgaa
     7561 gtgaaaatct aatcaatcct ctctaatgtg aaaacgaaca aatgacatgt ttgttcgttt
     7621 ttattagata gtaagattta cagaggaaaa tctaaaaaat agagacattt agactttcga
     7681 agtatgctat aataaagaaa ataaaaacaa gaggtttatc atgacaaaat tatatggaag
     7741 cttggaagcg ggcggtacaa agtttgtctg tgctgtcggt gatgaaaact ttaacgttgt
     7801 agaaaaaaca caatttccaa caacaactcc aatcgaaaca atcgataaaa ccattgagtt
     7861 cttctcaaaa ttcgataacc ttgctggtct tgcagttggt tcatttgggc cgattgatat
     7921 tgacaaaaac tcaaaaactt atggctttat cacgacgact ccaaaaccaa actgggcaaa
     7981 tgtggacttg cttggtgccc ttcgtcgcgc cctaaacgtg ccaatgtact tcacaacaga
     8041 cgtaaacagc tctgcttacg gtgaaatggt tgctcgtaac aatgctggtg gtcgtatcga
     8101 aaacttggtt tactacacaa tcggtacagg tatcggtgca ggcgtcatcc aacgtggtga
     8161 gtttatcggt ggtgtgggtc accctgaaat gggtcattat tatgttgcta gacacccaat
     8221 ggatattgaa aaagagttta agggtgtttg tcctttccat aagggatgtc tagaaggtta
     8281 tgcagctggt acaagtttgg aagctcgtac aggtgtacgt ggggaaacta ttgaactcaa
     8341 caaccctgtt tgggatgttc aagcctacta tatcgctcaa gctgcggtta atgcgacagt
     8401 gactttccgc ccagacgtga ttgtctttgg tggaggggtc atggctcaac aacatatgct
     8461 ggaccgtgtc cgtgagaaat ttacatctct tcttaatggt tacctaccag taccagatgt
     8521 gcgtgactat atcgtgacgc cagcagtcgc aggaaatggt tctgccacac ttgggaactt
     8581 tgttcttgca aaagaagttt caaaataaag cacaaaatcc gcttgggaaa ccaaacggat
     8641 tttgtggttg aaaacaatat ttttgagcct ttccttaagt gattacggac ggtagcgact
     8701 ttcttcgaaa ttccatacct aaactttgag cctagtgtct caaagtttcc gaatacctga
     8761 aaccattaga tttcaggtgt ttctatcacg gcggaaaggc taggtaagtc ctcgcctcac
     8821 tatcgaaaaa tatgcataat ttccatacca aagtatattt agtatcaagc tatagttaat
     8881 ctacgcttta aaaatccact tgggaaacca aacggatttt ttacgtgtca aagcatttgc
     8941 cagaaaaagt caataatata ggtcagaccg taggtataaa ccacgagggt aaagggtttg
     9001 gtcagaatga tgatagtcat gtagcgcttg aaactcatct tggtcagggc ggccagcata
     9061 cagagaaagt cagctgggct aatgggccaa atcatcataa aaataaagaa gcggtcaaaa
     9121 cgattgccct tatctagcca gccgatgtac ttgtcgtagg tgcgcttgct gacgacagac
     9181 tggacaaagg cagctccgta taggcgcact agataaaaga taatggcaca gccaatcacg
     9241 atgccgatat agttgtagat agtcccgatg atgtgcccgt agataaagac cccagccacc
     9301 gaggtcaagg cccctggaat gatagggacg acagtctgta aaatctgtaa aaagataaag
     9361 agaggtggac cccagatgcc tgcctgctgg ataaaggcag agagggtttc cttggattgt
     9421 aaaatcccag cctgataagc ccaaatacag aaaatcaaac tcccaacccc accgacaatc
     9481 gatgagatat tgataactcg ctgcagaagg ggagaaacag ctttcatttg tttatcctgt
     9541 gacatgtaaa ctcctcatag atagtatatt tctactatac catattttgg aggagaaagg
     9601 gggaatgagt cttttttgat aaggaataga aagtaaatag cccttcgaga cttagaatag
     9661 gtctgtcaac taaaggttat atgcattcta ggtttcgaat aatcgcaatt ccataaattt
     9721 aaaagtattc taagtttcga atagcttatc tgagataatt ttgaggcatt cgcgctttcg
     9781 aatagtcatt ttccctaaag tttatatgta ttctgaagct agaaagacta aaatataaaa
     9841 gtagtaagag tctctctctg atggacgtaa tttatggcta gtatgaatcc tttgaattgt
     9901 tagttaattt taatattaat cagttctgaa ataattacta aatatagtta gatggtgagt
     9961 taattattgc taaatttcaa gttcaagtta gtcatccaga agtcttctct gggggacttg
    10021 tagttcaagc attttttagg atagttgtta atccactttt cgataaatgc gacttccttt
    10081 gtagttgttt tcttagttcc ctgaggtaac catctatgaa tgagcctgtt gtgattttca
    10141 ttagatccta tagtgatgtg cataatagat atagcagatt gaaactagaa tagtatacat
    10201 ctacttctaa aacattgtta gaaatcaatt tgactgtcct gatcaatttg tcctgttttt
    10261 atttcatttt actatataat ccttagaaaa catatcggac aagcggttga attccgttcc
    10321 gtccattagt agataaagga ttactccttc ttgaaatgac agatgaactt gtttcttata
    10381 aaaatgttgt ctgaaactat tagccttttt ccagaagttt tagaggaaga aaactttacg
    10441 aggaaaaagg agttactcaa ataacaattc aggattaaaa atagacagtt gaggagcgga
    10501 aggtataaat taagtttgct actgtataat ggatttatca ct
//
LOCUS       DAVBCW010000065        10525 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVBCW010000065 DAVBCW010000000
VERSION     DAVBCW010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10525
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.065"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(80..1033)
                     /locus_tag="VQ514_001415"
     CDS             complement(80..1033)
                     /locus_tag="VQ514_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HET2500164.1"
                     /translation="MSISQRTTKLILATCLACLFAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
     gene            complement(1101..2310)
                     /gene="sstT"
                     /locus_tag="VQ514_001416"
                     /pseudo
     CDS             complement(1101..2310)
                     /gene="sstT"
                     /locus_tag="VQ514_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            2449..3009
                     /locus_tag="VQ514_001417"
     CDS             2449..3009
                     /locus_tag="VQ514_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HET2500165.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            3066..3974
                     /locus_tag="VQ514_001418"
     CDS             3066..3974
                     /locus_tag="VQ514_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HET2500166.1"
                     /translation="MVLEKQLGHGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLESHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            3926..4516
                     /locus_tag="VQ514_001419"
     CDS             3926..4516
                     /locus_tag="VQ514_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HET2500167.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            4519..5625
                     /gene="yqeH"
                     /locus_tag="VQ514_001420"
     CDS             4519..5625
                     /gene="yqeH"
                     /locus_tag="VQ514_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HET2500168.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGTAKVAVWAPEGVAVATRKAII"
     gene            5839..6150
                     /gene="yhbY"
                     /locus_tag="VQ514_001421"
     CDS             5839..6150
                     /gene="yhbY"
                     /locus_tag="VQ514_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HET2500169.1"
                     /translation="MSLASKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            6202..6831
                     /locus_tag="VQ514_001422"
     CDS             6202..6831
                     /locus_tag="VQ514_001422"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HET2500170.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKV
                     GTSYPVIWVDVPLMDISSSMVRDFLAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            6831..7424
                     /gene="yqeK"
                     /locus_tag="VQ514_001423"
     CDS             6831..7424
                     /gene="yqeK"
                     /locus_tag="VQ514_001423"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HET2500171.1"
                     /translation="MSYQDYINCSREALLEKMAEFLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSNQEFLALIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEVHTVGAGQMTDLDKVIYVADYIEHNRAFPGVDVAREIANLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKEN"
     gene            7425..7925
                     /locus_tag="VQ514_001424"
     CDS             7425..7925
                     /locus_tag="VQ514_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HET2500172.1"
                     /translation="MKTAFIIIDVQNILVETGFQTKSLLGKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            7938..8291
                     /gene="rsfS"
                     /locus_tag="VQ514_001425"
     CDS             7938..8291
                     /gene="rsfS"
                     /locus_tag="VQ514_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HET2500173.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            8611..9351
                     /locus_tag="VQ514_001426"
     CDS             8611..9351
                     /locus_tag="VQ514_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET2500174.1"
                     /translation="MATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLELA
                     CGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGKYD
                     FVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHENA
                     EDFAMLWDTYEDEVPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQAG
                     FKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            9361..10458
                     /locus_tag="VQ514_001427"
     CDS             9361..10458
                     /locus_tag="VQ514_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HET2500175.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVDILDRLGIDSLVFGTEEVLD
                     YQKIADLYTEKGAEMEKFVKNLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLTYILVQARENVLPEAIHVLGFTEKVRQHLKSLKGQVNLVSRIGKEPWDA
                     MTQKADQIYQLGKPSIAEQNFGRVPIRIETN"
BASE COUNT         3164 a         2070 c         2446 g         2845 t
ORIGIN      
        1 cgttttgagg ttgtggatag aactgacgaa gtcagtaaca tatatacagc aaggcgacgc
       61 tgacgtggtt tgaagagtat tactgtctat atttttggta aaaatcaact tttacttgga
      121 tgaaggtttt ggcttcacgt aggagttgaa gaagggtggc gcgggtttca aattcttctc
      181 ttgtcttggg cagactgcgg ttccggaaga cttccagata acgttcaatt tcatctagca
      241 aatcagaagc aggattggtc tggctcagtt gacctgcaat ttttgaaaag agttgcgcta
      301 agatcaggct ttcactggcg gcaaggtgac aagtgttaat ctgttgggcc atgtttctca
      361 ggatacgact ttgtcgttgt ctcatctcaa agtagtggat atggtagtcg gtctggtgaa
      421 agaggtggtc agagtgatcc aaatagacca gtctgagggc ttctttcaaa agcgtgtcta
      481 attctgctac cagctgtgct cggttgcgtc cgtctcctct ggataaatag tatttgaagc
      541 gctggaggat atcttttaac ttttcttcca ccagcgtgtg gtagtgctgg atttcctctt
      601 ctcgtgaagg catatagaga ttaacaagca aggcaaatcc tgtaccgata gcaaagagaa
      661 ggaattcgtt gactaggagg tctggagagg ttgactcttg aaccaagaga tggctaacca
      721 aaaccgtgct tggtgtgatg ccaatttccc agcccatctt gtaggctaaa ggaacgtaga
      781 aggccagata gaggccgaga ctccagatat gaaatccgct caagtgaaaa gctagaacac
      841 cgatagccag agctagaagc atagaaaaaa gacgattgcg agccagtttt aaagtacttc
      901 tacgcgtatc agataggctc aagagagcga taattccagc cgaaactgct gacgaaagat
      961 tgagaaaata agcaaacagg caggcaagac aggtagctaa gatgagcttg gtcgtacgtt
     1021 ggctaataga cataagaatt tcctaataag ttagaataaa agcgtaaaag acaagacatg
     1081 agcaggcttg ccttgatgag ttatttttta cgggttgctg cgtattcggc aacggcggta
     1141 aagaggacat ctgtagaaga gttaagggct gtttcacatg agtcttggat gacaccaatc
     1201 acaaaaccaa ccccaacaat ttgtatggca atatcgttag aaataccgaa aaggctacaa
     1261 gcaactggga taagaaggag ggaacctccg gcaatacctg aagcaccaca ggctgagata
     1321 gctgctacca cactgaggac aaaggctgtg gcaaagtcaa caggaattcc aagagtgtta
     1381 actgtaacaa gggtcaaaag gttaatggta atcgctactc cagccatatt gatagtagaa
     1441 ccgagtggga tagaaacaga ataggtatct gggttgagtc caaggtcatg gcagagtttc
     1501 atattgacag gaatgttagc cgcagaacta cgagtgaaaa aggctgtcac accgctgaca
     1561 cggaggcagt tccaaactag agggtaagga ttgcgtctca taaagaagaa ggcaatcaaa
     1621 gggttgacca caggggcaac aaaaagcata gtcgttacta atagaaccaa taaaataccg
     1681 tagttggcaa gccttccgac tcccttgtca gaaatggttt taaaaacaag accaaggatt
     1741 ccaaatggag tcagattgat gatccattcg acaattttag aagtcacgtc agcgatagtt
     1801 tttagcaatt ctttactatt tttactggct tctctcatag cgattccaaa aatgactgcc
     1861 caagataaga ttccaatata gttagcagta agcagggcgt tgactgggct gtcaaccagt
     1921 ttgagcaaga ggttgctgag gacctgccca atcccatctg gtggtgcaat ttcagtattg
     1981 gcactattta gggtaatttc aatagggacg atgaaacttg ctagtacagc tacaagagca
     2041 gcggcgaaag tccctatcat aggatataca agaaaacaac agttttcata ttgctatctt
     2101 gtcccttttg atgttgggaa agggcattgg caacgagagc aaagactagg ataggagcaa
     2161 cagcttttag acctccaacg aataaatccc cgagtagccc aatccctgag agattaggaa
     2221 gggtcagtcc taggattccc cacaaagcat accaatcaag atacgcttga caaggcttgc
     2281 cttattccaa gcatgaatga ttcttttcat aataatctcc tttttgtgta gtgattatga
     2341 ttatagtata aatgatagac aaaatcaaga attttctgtc tattttttga atatttatgg
     2401 agaatgagac tgatgaaaat atggtataat gaaataaagg agttttatat gcaaaaattt
     2461 attcaggctt atattgaaaa gctagatgtg acaaccatta tcgagaatat tctaaccaag
     2521 gtcatttctc ttttactgct tttgattgta ttttatattg ctaaaaaaat gcttcatacc
     2581 atggtgcaga gaattgtcaa accttctcta aaaatgtctc gtcatgatgt cggacgccaa
     2641 aaaaccatct cacgtttact agaaaatgtg tttaattata cgctatattt ctttttactc
     2701 tactgcattt tgtcgatttt aggtttgcca gtttctagtt tgctggctgg agctggtatt
     2761 gctggggtag cgattggtat gggagcccaa ggctttctgt ctgatgtcat caatggcttt
     2821 ttcatcctct ttgaacgtca actggatgtg ggagatgagg tcgttctgac aaatggaccg
     2881 attactgtat cgggtaaggt tgtcagtgtg ggaattcgta cgacacagct tcgtagcgag
     2941 gagcaagccc ttcactttgt ccctaaccga aatatcacag ttgttagcaa tttctcacgc
     3001 acagactaga cctgttattt taagtaattt gtggtacaat agagggagtt taataaggag
     3061 aaaagatggt tttagaaaag cagttgggcc atggttgtac ctggatagac ctagacctag
     3121 gaaagttgaa taaactagaa gacctttctg aaatttacgg tttggacaag gaaaccattg
     3181 aatacgcact ggatagaaac gagcgcgccc acatggacta ccaccgtgaa agtgggacgg
     3241 ttacctttat ctataatgtc ctagacgtaa aaaaggacaa ggcctactat gagacttttc
     3301 ccatgacctt tatcgtcgag catcgtcgcc tgattaccat tagtaatacc aagaacgcct
     3361 atgtcattga acagatgact cgttatctgg agagccatga cacgctttcg atttataagt
     3421 ttctctttgc cagtctggaa atcatcagca atgcctacta tcctgtcatt gagcagatgg
     3481 acaagagtag ggatgaggtc aatgacctct tgcgccagcg aactaccaag aaaaacctct
     3541 ttgccctgtc tgatttggag actggtatgg tttatctgac ggcagctgcc aaacaaaatc
     3601 ggattttgtt agagcatatt caaggtcatg ccttgtatcg tagttttgat gagattgaga
     3661 gagaacagtt tgatgatgcc atgattgagg ctcatcagct ggtatccatg acagacctaa
     3721 tctctcagat tttacagcag ctttcagcct cttacaacaa tattctaaac aataatctga
     3781 atgacaattt gacaaccttg actatcattt cagtcttgct agctgttttg gcagtcgtga
     3841 caggcttttt cggaatgaat gttcccttac ctttaacaga tgagccccat gcttggctct
     3901 atatcagttt ggctagtgca ggtttgtgga ttgttttatc cttgttacta aggaaaattg
     3961 cgaaaaaaag ttaagaaaag gagccagaat ggcgattgaa aattatatac cagattttgc
     4021 tgtggaagca gtctatgatc tgacagtccc aagcctgcag gcgcagggaa tcaaggctgt
     4081 tttggtcgat ttggataata ccctcattgc ttggaacaac cctgatggaa cgccagagat
     4141 gaagcaatgg ctacatgacc ttcgggacgc gggtattggc attatcgtag tgtcaaataa
     4201 caccaaaaaa cgcgttcaac gagcagttga gaaatttggg attgattacg tttactgggc
     4261 cttgaagccc ttcacatttg gtattgaccg tgccatgaag gaattccact atgacaaaaa
     4321 ggaagtggtc atggttggcg accagctcat gacagatata cgagcagccc accgtgcagg
     4381 gattcggtca attttagtca aacccttggt ccaacatgac tcaatcaaaa cgcagattaa
     4441 ccgaactcgt gagcgtcgtg ttatgagaaa aatcactgaa aagtacggac cgattacata
     4501 taaaaaagga atttaactat ggaagaaatt ctctgtattg gttgtggagc aaccattcag
     4561 acgacagata aagctggtct tggttttacc ccccagtcgg cacttgaaaa aggtttggag
     4621 actggcgaag tctattgcca acgctgtttc cgtctccgcc actacaatga aatcacagat
     4681 gtccagttga cgaacgatga tttcctcaag ctcttgcacg aggtgggaga cagtgatgcc
     4741 ttagtggtca atgtcattga tatctttgat tttaatggat ctgtcatccc aggtttacca
     4801 cgtttcgtct cgggcaatga tgtcctcttg gtaggaaata aaaaagatat ccttcctaag
     4861 tcagttaagt ctggtaagat tagccagtgg ctcatgaaac gtgcccatga agaaggtctt
     4921 cgtccagtcg atgtggtcct aacttcagca caaaataaac atgccattaa ggaagtcatt
     4981 gataagattg aacactaccg taagggccgc gatgtctatg tggtcggtgt gaccaacgtt
     5041 ggaaaatcaa ctctaatcaa tgctattatc caagaaatca cgggtgatca gaatgtcatc
     5101 actacttcac gcttcccagg gacaaccttg gacaaaatag agattccgct tgacgacgga
     5161 tcttatattt acgatacgcc gggaattatc caccgccacc agatggctca ctacttgacg
     5221 gccaaaaacc tcaagtatgt cagtcctaaa aaggaaatca agcctaagac ctatcagctt
     5281 aatcctgagc aaaccctatt tttaggtggt ttgggacgct ttgactttat agcaggagaa
     5341 aagcaaggat ttactgcttt ctttgataat gaactcaaac tccatcgtag caagcttgaa
     5401 ggagctagtg ctttctacga taagcacctg ggaactcttc tgacaccacc aaatagcaag
     5461 gaaaaagaag atttcccaag gctagtccag catgtcttta ccattaaaga taagacagac
     5521 ctagtcattt caggcctagg ctggattcgt gtaacaggca cagcaaaagt cgccgtctgg
     5581 gcaccagaag gcgtcgccgt cgccacacga aaagcaatta tttaaaacaa aatcgctgag
     5641 cgaacaaagt gagctcatat agcgatagtt attgccgtgg tgtgattgcc aatctgtaga
     5701 ttggcaaggg gcacaattga gaccaaggct caattgtgag gtcaggaaat ccatttttca
     5761 aagatgaaat ctttggaaaa ttagttccga ccgtccctca ccacctaaaa taactatcaa
     5821 aaaacaagga agaaaattat gtcattagca tcaaaacaac gtgccttcct caacagccag
     5881 gcacacaccc tcaaacctat catccaaatc gggaaaaatg gactcaacga ccaaatcaaa
     5941 accagcgtcc gtcaagctct tgatgcgcgt gaattaatca aggttactct cttacaaaac
     6001 acagatgaaa acatccacga agtagctgaa attttggaag aagaaatcgg tgtggataca
     6061 gtccaaaaaa taggacgcat cttgattttg tttaagcaat ctagcaagaa agaaaatcgc
     6121 aagatttcta agaaagtcaa agaaatctaa gaccgaaact ccaaataact gtttttatag
     6181 agaaataaag gggactagcc tatgacaatc gaactattga ctccctttac caaggtagag
     6241 ttggagccag aaatcaagga gaaaaaacgc aaacaagttg ggattttagg ggggaatttt
     6301 aaccctgttc acaatgccca tctcattgtt gcggatcaag tacggcaaca gttgggactg
     6361 gatcaagttc ttctcatgcc tgaataccaa cctcctcacg ttgataaaaa ggaaaccatc
     6421 cctgaacacc atcgtctcaa gatgcttgag ttggcaattg agggaattga cggcctagtc
     6481 attgaaacca ttgagttgga gcgcaagggt atttcctaca cctacgacac catgaagatt
     6541 ttgacagaga agaatccaga tacggattat tactttatca tcggtgccga catggttgac
     6601 tatctgccta agtggtaccg aattgatgaa ctggttgaca tggttcagtt tgtgggggtt
     6661 cagcgtccac gctacaaggt agggacttcc tatccagtta tctgggtgga cgtaccgctc
     6721 atggatatct cgtccagcat ggtgcgggac ttccttgccc aaggtcggaa acccaacttt
     6781 ctcctacctc agccagtgct agactacatc gagaaggagg ggctctactg atgtcctatc
     6841 aagactatat caactgctcc cgtgaggcct tgttggaaaa aatggcagag tttctacctg
     6901 agaaacgtct aacccattgt ctaggtgtgg agcgtgccgc tatggaatta gctcagcgct
     6961 ttggtgtaga tgttgagaaa gcaagtctag caggtcttct tcatgactat gctaaaaagt
     7021 tgtcaaatca ggaatttcta gccttgattg accgttacca gctagaccct gacctcaaaa
     7081 actggggcaa taatgtctgg catggtatgg tcggaattta caaaattcag gaagatttgg
     7141 atttgcatga ttcagaaatc ctgcgagcca ttgaagtcca tacagtcggt gctggtcaaa
     7201 tgacagacct agataaggtc atctacgtcg cagactatat cgagcacaat cgagcctttc
     7261 ctggagtaga tgtggcgcgt gagattgcaa atctatcgct caataaggcg gtggcctacg
     7321 aaacagctcg taccgtggag tatctagctc atcagggatt ccccatctat ccccaaaccc
     7381 ttgaaaccta taacgccttt gtgcactatt tgaaagagaa ctaaatgaag acggctttta
     7441 taatcattga tgttcagaat attttagtag aaactgggtt ccagacaaaa agtctattgg
     7501 gaaaaatttc ttatttacaa aaccaggcta gaagcaagaa tattgaaatt atctatgttc
     7561 aacatattga gaactctgaa gctcaaacat cagaagattg gcagttatct gcgcttttaa
     7621 atcgaaaacc tgctgaaaag gtctttcaga agaagtataa cagtattttc aaagaaactg
     7681 gcttaaaaga atacttggat aagcagggga ttgaaaaatt agttttatgt ggtatgcaga
     7741 cagaatattg tgtggatacc tctgtcaagg ttgcctttga atatggctat cagcttattg
     7801 ttccagaagg tgctgtcaca acctttgatg gggatgacat tccagcagaa acgattaatg
     7861 aattttatga ggacatttgg gaggagcgct ttgcagatgt tctagattac aaacatattt
     7921 tctaaaaaga ggattaaatg aacgaaaaag aattactaga actagtcgtg aaagcggctg
     7981 atgagaaacg tgcggaggat atcctcgcac ttgatgtaca agatttgact agtgtgacgg
     8041 actactttgt catcactagc tcaatgaata gccgtcagtt ggacgctatc gcagctaata
     8101 tccgtgaaaa agtagctcaa gcaggcttta aaggtagcca tgtcgaaggc gatgcagctg
     8161 gaggctgggt cttactggac cttggtgctg ttgtcgtgca tatcttttca gaagaaatgc
     8221 gtgcccatta taatctagag aagctatggc atgaggcgaa ttcagtagat atttcagaag
     8281 ctcttgctta gaagatgaac tcagttgtag tcaactgggt ttctttgctt attttagaaa
     8341 aattgataga aaggtaaagg gcctcgtgtt tgtttcattt cgaaactgaa cacgagctaa
     8401 aagctgagat aaaaagtaac tttctttgta tctgacgata gggaagaaag tttcctatgt
     8461 actctttgtt tttaaggctc tttgtatctt agaaaggatt tgaacccgtc ctgtaattct
     8521 cggaaaatag atagccttcc ttggagctcg gctccatcgt aaggctccta attttctatc
     8581 gaattacggg cggacttggt atcatgtttt atggcgactt atgaaacctt tgcggcggtc
     8641 tatgatgcgg tcatggacga tagtttatac gacaaatgga cgaatttttc tctgcgtcat
     8701 ttgcctaaga ccaaggagag aaagaaactc ttggaattgg cttgtgggac aggaattcaa
     8761 tcagtgcgct tttctcaggc tggttttgat gtgactggac ttgacttgag tgcggatatg
     8821 ttgaagattg cggagaagag agcaacttca gccaagcaaa agattgcttt tatagaaggt
     8881 aatatgctga atttgtccaa ggcagggaaa tacgattttg tcacgtgtta ttcggactct
     8941 atctgctata tgcaggatga ggtggaagta ggggacgtct ttaaggacgt gtacaatgcg
     9001 ctgaatgaag agggagtttt catctttgac gtgcactcga cctaccagac agatgaagta
     9061 ttccctggct attcctacca tgaaaatgcg gaagattttg ccatgctttg ggatacatat
     9121 gaggacgaag ttcctcactc catcgtgcat gagctgactt tctttatcaa ggaggctgac
     9181 ggttccttta gtcgccacga tgaagtacat gaggagagga cctatgagat cttgacctat
     9241 gatatcttgc tggaacaggc tggatttaag tccttcaaac tctatgcaga ctttgaggat
     9301 aaggagccaa cagaaactag cacccgttgg ttttttgtgg cgcagaagta ggagattagc
     9361 atgaccataa caggtattat cgcggagttc aatccttttc ataatggcca taaatacctg
     9421 ctggatcagg cagagggact gaaaattgtt gccatgtcag ggaatttcat gcagcgtgga
     9481 gagcctgcta ttgtggataa gtggacaagg gcccagatgg cgctggaaaa tggcgcagac
     9541 ttggtagtgg aattgccctt tttagtcagt gttcaggcag ctgatttctt tggtcaagga
     9601 gctgtggata tcttggatcg cttgggcatt gatagtctag tttttgggac agaggaagtt
     9661 ctggattacc agaaaatcgc tgacttatac acagagaaag gtgctgagat ggagaaattt
     9721 gtgaaaaatc tgcctgattc cctttcctat ccccagaaaa cccaagctat gtggaaggaa
     9781 tttgctggtc ttgatttttc aggtaatacc cccaatcatg ttcttgctct ggcctatgcc
     9841 aaggcggttg caggacgaaa tatcaagctg catccgattc agcgtcaggg ggcaggttac
     9901 cattctgtga ataaagatgt ggactttgcc tcggcgacag ccctccgtca acatcagaag
     9961 gaccaagatt tcttagaacg ttttatgcct tctgttgccc tatttgagca ggccagtaag
    10021 gtgatctggg aagactattt tcccttgctc cgctatcaaa tcttgtcaaa tcccgaccta
    10081 accaccatct atcaggtcaa tcaagaaatg gctgtgcgca ttaaagaagc tattaaaaca
    10141 gcccagtctg tggaagaatt ggtcgagtta gtgactacca aacgttacac caaggcgcgt
    10201 gtcagacgcc tattgaccta tattttggta caggctagag aaaatgtctt gccagaagcc
    10261 attcatgtcc ttggatttac tgaaaaagtc aggcaacatc tcaagtctct gaaaggacag
    10321 gtcaatctag tcagccgaat tggcaaagaa ccttgggatg ctatgaccca aaaagcagac
    10381 cagatttacc aactagggaa gccaagtata gcagagcaaa attttggaag agtgccgatt
    10441 agaatagaaa caaactaagt ctaccgaaag gtaggctttt tgagattttt tacgaataat
    10501 actcttcgaa aatcaaattc aaatc
//
LOCUS       DAVBCW010000066        10438 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVBCW010000066 DAVBCW010000000
VERSION     DAVBCW010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10438
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.066"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            210..623
                     /gene="rpsL"
                     /locus_tag="VQ514_001428"
     CDS             210..623
                     /gene="rpsL"
                     /locus_tag="VQ514_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HET2500176.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            643..1113
                     /gene="rpsG"
                     /locus_tag="VQ514_001429"
     CDS             643..1113
                     /gene="rpsG"
                     /locus_tag="VQ514_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HET2500177.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            1538..3619
                     /gene="fusA"
                     /locus_tag="VQ514_001430"
     CDS             1538..3619
                     /gene="fusA"
                     /locus_tag="VQ514_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET2500178.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     SKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            3723..8114
                     /locus_tag="VQ514_001431"
     CDS             3723..8114
                     /locus_tag="VQ514_001431"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HET2500179.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFQTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPVEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHDLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNEIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNDYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYRSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            8216..8479
                     /locus_tag="VQ514_001432"
     CDS             8216..8479
                     /locus_tag="VQ514_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HET2500180.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQVLTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            8472..8750
                     /locus_tag="VQ514_001433"
     CDS             8472..8750
                     /locus_tag="VQ514_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HET2500181.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            8771..8923
                     /locus_tag="VQ514_001434"
     CDS             8771..8923
                     /locus_tag="VQ514_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500182.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            8945..10186
                     /locus_tag="VQ514_001435"
     CDS             8945..10186
                     /locus_tag="VQ514_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HET2500183.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEGFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENVGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            10196..10426
                     /locus_tag="VQ514_001436"
     CDS             10196..10426
                     /locus_tag="VQ514_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET2500184.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
BASE COUNT         3073 a         2024 c         2471 g         2870 t
ORIGIN      
        1 cagaaattga tctctaacat taccaataaa aatgaaaaat atcttgacaa gcaagggaaa
       61 attttgttac aataatagac ggtacttttt acttttggtc tctcaagagt gtacagggac
      121 gtgctgacaa atgttgcaaa agtacacaca gatgatatct gtcaccaagt gtatcatcac
      181 caaaaataaa aaaacacagg agaatgtaga tgcctacaat taaccaattg gttcgcaaac
      241 cgcgtaaatc aaaagtagaa aaatctaaat caccagcttt gaacgttggt tacaatagtc
      301 ataaaaaagt tcaaacaaac gtttcttcac cacaaaaacg tggtgttgca actcgtgttg
      361 gaacaatgac acctaaaaaa cctaactcag cccttcgtaa attcgctcgt gtacgtttga
      421 gcaaccttat cgaagttact gcctacatcc caggtatcgg acacaacttg caagagcaca
      481 gcgtggtgct tcttcgcggt ggacgtgtaa aagaccttcc aggggtacgt taccatatcg
      541 tccgtggtgc acttgatact gcaggtgtta acgatcgtaa acaaggccgt tctaaatacg
      601 gtactaaacg tccaaaagca taaggaaagg ggataaagag aaatgagtcg taaaaataga
      661 gctccaaaac gtgacgtatt gccagatccg ctatacaatt cacaactagt tactcgtctt
      721 atcaaccgcg ttatgcttga tggtaaacgt ggtactgctg cttcaatcgt ttacggtgct
      781 tttgagcaaa tcaaagaagc tactggcaac gatgcacttg aagtatttga aacagctatg
      841 gaaaacatca tgcctgtact tgaagtacgt gcacgtcgtg ttggtggttc taactaccaa
      901 gtcccagttg aagttcgtcc agaacgtcgt acaacacttg gacttcgttg gttggtaaca
      961 atcgctcgtc ttcgtggtga acacacaatg caagaccgtc ttgcaaaaga aatcttggat
     1021 gctgctaaca acactggtgc agctgttaag aaacgtgaag atactcaccg tatggctgaa
     1081 gctaaccgtg cattcgcaca cttccgttgg taagatagga tgcgaaagcg ttaagaaagt
     1141 cccagagaaa atagggaatc gaagcaggtt gcggttgcaa ccaatgagat tcatcttttt
     1201 ctccagactt ttagcttgag ctcaactaaa tcatgatgct aggcacggta aggatgcaag
     1261 gtgaaaatag gaaactgacg cagtattcaa cgaatacaag gagttttatc tttttcacgc
     1321 agcatcccgt tccagctcac atcggctaac taactttagc ccgggttcaa attagctaaa
     1381 tcgattagta ttagctataa ctcagcttac catctcgtaa gttgaaacca acaatagcat
     1441 gaaaacattg agaacgggta ggtcctgcct atccgttttt attaaaatcg tgttataata
     1501 gaatagaaat caaaaataaa taggagaaac aaacctcatg gcacgcgaat tttcacttga
     1561 aaaaactcgt aatatcggta tcatggctca cgtcgatgcc ggtaaaacaa caactactga
     1621 gcgtattctt tactacactg gtaaaatcca caaaatcggt gaaactcacg aaggtgcgtc
     1681 acaaatggac tggatggagc aagagcaaga acgtggtatc acgatcacat ctgctgcgac
     1741 aacagctcaa tggaacaacc accgcgtaaa catcatcgac acaccaggac acgtggactt
     1801 cacaatcgaa gtacaacgtt ctcttcgtgt attggatggt gcggttaccg ttcttgactc
     1861 acaatcaggt gttgaacctc aaactgaaac agtttggcgt caagcaactg agtacggagt
     1921 tccacgtatc gtatttgcca acaaaatgga caaaatcggt gctgacttcc tttactctgt
     1981 aagcacactt cacgatcgtc ttcaagcaaa tgcacaccca atccaattgc caatcggttc
     2041 tgaagatgac ttccgtggta tcattgactt gatcaagatg aaagctgaaa tctatactaa
     2101 cgaccttggt acggatatcc ttgaagaaga catcccagct gaataccttg accaagctca
     2161 agaataccgt gaaaaattga ttgaagcagt tgctgaaact gacgaagaat tgatgatgaa
     2221 atacctcgaa ggtgaagaaa tcactaacga agaattgaaa gctggtatcc gtaaagcgac
     2281 tatcaacgtt gaattcttcc cagtattatg tggttcagcc ttcaaaaaca aaggtgttca
     2341 attgatgctt gatgcggtta tcgactacct tccaagtcca cttgacatcc cagcaatcaa
     2401 aggtattaac ccagatacag acgctgaaga aactcgtcca gcatctgacg aagagccatt
     2461 tgcagctctt gccttcaaga tcatgactga cccattcgta ggtcgtttga cattcttccg
     2521 tgtttactca ggtgttcttc aatcaggttc atacgtattg aatacttcta aaggtaaacg
     2581 tgaacgtatc ggacgtatcc ttcaaatgca cgctaacagc cgtcaagaaa tcgacactgt
     2641 ttactcaggt gatatcgctg ctgccgttgg tttgaaagat actacaactg gtgactcatt
     2701 gacagatgaa aaatctaaaa tcatccttga gtcaatcaac gttccagaac cagttatcca
     2761 attgatggtt gagccaaaat ctaaagctga ccaagacaag atgggtatcg cccttcaaaa
     2821 attggctgaa gaagatccaa cattccgcgt tgaaacaaac gttgaaactg gtgaaacagt
     2881 tatctcaggt atgggtgaac ttcaccttga cgtccttgtt gatcgtatgc gtcgtgagtt
     2941 caaagttgaa gcgaacgtag gtgcgcctca agtatcttac cgtgaaacat tccgcgcttc
     3001 tactcaagca cgtggattct tcaaacgtca gtctggtggt aaaggtcaat tcggtgatgt
     3061 gtggattgaa tttactccaa acgaagaagg taaaggattc gaattcgaaa acgcaatcgt
     3121 cggtggtgtg gttcctcgtg aatttatccc agcggttgaa aaaggtttgg tagaatctat
     3181 ggctaacggt gttcttgcag gttacccaat ggttgacgtt aaagctaagc tttatgatgg
     3241 ttcatatcac gatgtcgact catctgaaac tgccttcaag attgcggctt cactttccct
     3301 taaagaagct gctaaatcag cacaaccagc tatccttgaa ccaatgatgc ttgtaacaat
     3361 cactgttcca gaagaaaacc ttggtgatgt tatgggtcac gtaactgctc gtcgtggacg
     3421 tgtagatggt atggaagcac acggtaacag ccaaatcgtt cgtgcttacg ttccacttgc
     3481 tgaaatgttc ggttacgcaa cagttcttcg ttctgcatct caaggacgtg gtacattcat
     3541 gatggtattt gaccactacg aagatgtacc taagtcagta caagaagaaa ttattaagaa
     3601 aaataaaggt gaagactaat ccgtcctcac tctagaagga agtcacttag tggcttcctt
     3661 ttgtctttag aaaatacctc taaatatggt aaaatagtag aagaataatg tgaggaaaat
     3721 gaatgtcaaa tagttttgaa attttgatga atcaattggg gatgcctgct gaaatgagac
     3781 aggctcctgc tttagcacag gccaatattg agcgagttgt ggttcataaa attagtaagg
     3841 tatgggagtt tcatttcgta ttttctaata ttttaccgat tgaaatcttt ttagaattaa
     3901 agaaaggttt gagcgaagaa ttttctaaga caggcaataa agctgttttt gaaattaagg
     3961 ctcggtctca agaattttca aatcagctct tgcagtccta ctatagggag gctttctctg
     4021 aaggtccatg tgctagtcaa ggttttaagt ccctttatca aaatttgcaa gttcgtgctg
     4081 agggtaatca gctatttatt gaaggatctg aagcgattga taaggaacat tttaagaaga
     4141 atcatcttcc taatttagcc aaacaacttg aaaagtttgg ttttcaaact tttaactgtc
     4201 aagtcgagaa gaatgatgtc ctgacccaag agcaggaaga ggcctttcat gctgaaaatg
     4261 agcagattgt tcaagctgcc aatgaggaag cgctccgtgc tatggaacaa ctggagcaga
     4321 tggcacctcc tccagtggaa gagaaaccag tctttgattt tcaagcgaaa aaagctgcag
     4381 ctaaacccaa actggataag gcggagatta ctcctatgat cgaagtgacg acagaggaaa
     4441 atcgtctggt atttgaaggg gttgtttttg atgtggagca aaaagtgact agaacaggtc
     4501 gtgttttaat caactttaaa atgacggact atacttcaag tttttctatg caaaagtggg
     4561 ttaaaaacga ggaagaggcc cagaagtttg acctcatcaa gaagaattct tggctccgag
     4621 ttcgagggaa tgtggagatg aataacttca cacgcgattt gactatgaac gtacaggatc
     4681 tgcaggaagt tgttcactat gagcggaagg atttgatgcc agaaggtgag cgtcgggttg
     4741 agtttcatgc tcatactaac atgtcgacta tggatgcttt gccagaggtc gaagagattg
     4801 ttgcaacagc tgctaagtgg ggacacaagg cggttgctat cacagaccat gggaatgtcc
     4861 agtcctttcc acatggctat aaggcggcta agaaagcggg aatccagctg atctatggga
     4921 tggaagccaa tatcgtggag gaccgtgtcc ctatcgtcta taacgaagtg gagatggact
     4981 tatcagaagc aacctacgtg gtctttgacg tggaaacgac gggactttca gctatctata
     5041 atgacttgat tcaggttgcg gcctctaaga tgtacaaggg gaatgttatt gctgaatttg
     5101 atgaatttat caatcctggg catcccttgt cagcttttac tacagagtta actggaatta
     5161 cagatgatca tgtcaaaaat gccaaaccac tagaacaagt tttgcaagaa ttccaagaat
     5221 tttgcaagga tacggtccta gttgcccaca atgctacctt tgacgttggc tttatgaatg
     5281 ctaattatga gcggcatgac cttccaaaga ttagtcagcc agttattgat acgctggagt
     5341 ttgctagaaa cctctatcct gagtataaac gccatggttt ggggcctttg accaagcgtt
     5401 ttggtgtggc cttggaacat caccacatgg ccaactacga tgcggaagcg actggtcgtc
     5461 tgcttttcat ctttatcaaa gaggtagcag aaaaacatgg tgtgaccgat ttagctagac
     5521 tcaacattga tctaatcagt ccagattctt acaaaaaagc tcggatcaag catgcgacca
     5581 tctatgtcaa gaatcaggta ggtctaaaaa atatctttaa gctggtttcc ttgtctaata
     5641 ccaagtattt tgaaggagtg ccacggattc cgagaacggt tctagatgcc catcgagagg
     5701 gcttgatttt aggttcagcc tgttcagagg gtgaagtttt tgacgtggtc gtttctcaag
     5761 gtgtggatgc ggcggttgag gtggccaagt attatgattt tatcgaggtc atgccaccgg
     5821 ctatctatgc acccttgatt gccaaagagc aggtcaagga tatggaggaa ctccagacca
     5881 ttatcaagag tttgatagag gttggagacc gccttggcaa gcctgttctg gctacgggaa
     5941 atgttcacta tatcgaaccg gaagaagaga tttatcgtga aattatcgtc cgtagtttgg
     6001 gacagggtgc gatgattaat cgaactatcg gtcatggtga acatgcccaa ccagcaccac
     6061 ttccaaaggc tcattttcga acgactaatg agatgttgga tgaatttgcc tttttgggag
     6121 aggaactggc tcgtaaactg gttattgaaa acaccaatgc cttggcagaa atatttgaac
     6181 ccgttgaagt cgttaagggt gacttgtata cgcctttcat cgacaaggct gaagaaacag
     6241 ttgctgagtt gacctataag aaagcttttg agatttatgg aaatccgctg ccagatattg
     6301 ttgatttgcg gattgaaaaa gaattaacat ccatactggg gaatggattt gctgtgattt
     6361 atctggcatc gcagatgctg gtgcaacgtt ctaatgaacg gggttatttg gttggttctc
     6421 gtgggtctgt cggatctagt ttcgttgcga ccatgattgg gattacggag gtcaatcctc
     6481 tctctcctca ctatgtctgt ggtcagtgtc agtacagtga gtttatcaca gatggttcgt
     6541 acggttcagg atttgatatg ccccataagg actgtccaaa ctgtggtcac aaactcagta
     6601 aaaacggaca ggatattccg tttgagacct tccttggttt tgatggggat aaggttcctg
     6661 atattgactt gaacttctcg ggagaagatc agcctagcgc ccacttggat gtgcgtgata
     6721 tctttggtga agaatatgcc ttccgtgcgg gaacggttgg tacggtagct gccaagactg
     6781 cctatggatt tgtcaagggt tacgagcgag attatggcaa gttttatcgt gatgcagagg
     6841 tggaacgcct cgctcaagga gcggcgggtg tcaagcggac aacaggccaa cacccggggg
     6901 gaatcgttgt tattccgaac tacatggatg tctacgattt tacgcctgtc cagtatccag
     6961 cagatgatgt cacggctgaa tggcagacca ctcactttaa cttccacgat atcgatgaga
     7021 acgtcctcaa actcgatgta ctgggacatg atgatccgac catgattcga aaacttcagg
     7081 atttgtctgg tattgaccct aatgaaattc ctatggatga cgaaggcgtg atggcactct
     7141 tttctgggac tgatgtgcta ggggtaacac ctgaacaaat tggaacgcct acgggtatgt
     7201 tggggattcc agagtttgga acaaatttcg tacgtggaat ggtagacgaa acccatccga
     7261 caacctttgc ggaattgctt cagctgtctg gtctgtcaca cggtactgac gtttggttgg
     7321 ggaatgctca ggatctgatt aagcaaggaa tagcggacct atcgactgtt atcggttgtc
     7381 gggacgacat catggtttac ctcatgcatg cgggtctgga acctaagatg gcctttacca
     7441 ttatggaacg ggtacgtaag ggtttgtggc taaagatttc agaagaggag agaaatgact
     7501 atatcgaagc catgaaggct aataaggtgc cagagtggta tatcgaatcc tgtgggaaaa
     7561 ttaagtacat gttccctaag gcccatgcgg cagcctacgt tatgatggcc ttgcgtgtag
     7621 cttacttcaa ggttcaccat cctatttatt actactgtgc ttacttctcc attcgtgcta
     7681 aggcttttga tatcaagacc atgggtgcgg gcttggaggt catcaagcgc agaatggaag
     7741 aaatctctga aaaacggaag aacaatgaag cctctaatgt ggaaatcgat ctctatacaa
     7801 ctcttgagat tgtcaatgag atgtgggaac gaggtttcaa gtttggtaaa ttagatctct
     7861 accgtagtca ggcgacagag ttcctcatcg acggggatac ccttatccca ccatttgtag
     7921 caatggatgg tctgggagag aacgttgcca agcaactggt gcgggcgcgt gaagagggag
     7981 aattcctctc taaaacagaa ctacgcaagc gtggtggact ctcatcaacc ttggttgaaa
     8041 agatggatga gatgggtatt cttggaaata tgccagagga taaccagttg agtttgtttg
     8101 atgagttgtt ttaaaaaatt gcttaataat ctattaaaag aggctaacgt atatccaata
     8161 gatttacatt agctttcttt tttgttaaaa tagtctatgg aaatagggtg agagtatgtc
     8221 aaagatgagt ataagcatcc gtctggatag tgaggttaag gagcaggccc aacaggtgtt
     8281 tagtaatctg ggaatggata tgacaacagc tattaatatt ttccttcgtc aggcaattca
     8341 atatcaggga ttaccttttg atgttagact agacgaaaat cggaagttgc tccaagtgtt
     8401 aacagattta gaccaaaatc gtaatatgag ccagtctttt gaatcagtct cagatttgat
     8461 ggaggactta cgtgcttaag attcgttatc ataaacagtt taaaaaagat tttaagttgg
     8521 ctatgaagcg tggtttgaag gccgaattat tagaagaagt tttgaatttt ctggttcaag
     8581 aaaaagaaca tcctgccaga tatcgtgatc attcattgac ggcatccaag cattttcaag
     8641 gagttcgtga atgccatacc cagccagatt ggcttttggt ttataaagta gacaagtcgg
     8701 aattgatttt aaatttgctg aggacaggca gtcacagtga tttattttaa tctattttaa
     8761 gggggttctc atgaaactaa gaatatttgc ggaagataag ccggctaaga aggtatttga
     8821 atatcaatta gaacttgctg atcgtacaat tcttctatcg acagcactct tgtcaggtgc
     8881 tattgcttta gcaggaatct tttctgcctt gaaagaaaaa taaaaataga aaagagaaaa
     8941 cagaatggtt ttaccaaatt ttaaagaaaa tctagaaaaa tatgcgaaat tgttggttgc
     9001 gaacggaatt aacgtgcaac ctggtcacac tttggctctc tctattgatg tggagcaacg
     9061 tgaattggca catctaatcg tgaaagaagc ttatgccttg ggtgcgcatg aggtcatcgt
     9121 tcagtggaca gatgatgtca ttaaccgtga gaaattcctc catgccccga tggagcgttt
     9181 ggacaatgtg ccagaataca agattgctga gatgaactat cttttggaga acaaggctag
     9241 ccgtcttggg gttcgttcat ctgatccagg tgccttgaac ggagtggacg ctgacaagct
     9301 ttcagcttct gctaaagcta tgggacttgc catgaagcct atgcgtatcg caactcaatc
     9361 taacaaggtt agctggactg tagcagctgc agcaggactt gagtgggcta agaaagtctt
     9421 cccaaatgct gcgagcgacg aagaagcagt tgatttcctt tgggaccaaa ttttcaaaac
     9481 ttgccgtgtc tatgaagcag atcctgttaa ggcttgggag gaacatgcag ccattctcaa
     9541 gagcaaggca gatatgctta ataaggagca attttcagcc cttcactaca cagcgccagg
     9601 aacagattta acacttggtt tgccaaagaa ccacgtttgg gaatcagctg gtgctgtcaa
     9661 tgcacagggc gaaggattct tgccaaatat gccgacagaa gaggtcttca cagcgcctga
     9721 cttccgtcgt gcagatggtt atgtcacttc tacaaaaccg cttagctaca acggaaatat
     9781 cattgaaggc attaaggtga cctttaagga tggacaaatc gtagatatca ctgctgagaa
     9841 gggtgatcag gttatgaaag accttgtctt tgaaaatgtg ggtgcgcgtg ccttgggtga
     9901 atgtgccttg gtaccagatc caagtccaat ttctcagtca ggcattacct tctttaacac
     9961 ccttttcgat gaaaatgcgt caaaccactt ggctatcggt gcagcctatg cgactagcgt
    10021 tgttgatgga gcggagatga gcgaagagga gcttgaagct gcagggctta accgttcaga
    10081 tgttcacgta gactttatga ttggttctaa ccaaatggat atcgatggta ttcgtgagga
    10141 tggaacgcga gtacctcttt tccgtaatgg gaattgggca aattaaggag ataatatgtt
    10201 aggaagtatg ttcgttggtc tcctagtggg atttttagca ggtgctatga ccaatcgtgg
    10261 agagcgaatg ggatgttttg gaaaaatgtt tctcggttgg atcggagcct ttctaggtca
    10321 cttgctcttt ggaacttggg ggccagtttt atcaggaaca gctattatcc cagcgatttt
    10381 aggagccatg attgttttag ctattttttg gagacgagga agttaatttc ctaaatct
//
LOCUS       DAVBCW010000067        10379 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVBCW010000067 DAVBCW010000000
VERSION     DAVBCW010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10379
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.067"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(<1..908)
                     /gene="cbpD"
                     /locus_tag="VQ514_001437"
     CDS             complement(<1..908)
                     /gene="cbpD"
                     /locus_tag="VQ514_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HET2500185.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRV
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGGTHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSGTVIDYYYPGEKVHYDQIL"
     gene            complement(997..1263)
                     /locus_tag="VQ514_001438"
     CDS             complement(997..1263)
                     /locus_tag="VQ514_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500186.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            complement(1265..2617)
                     /gene="dnaB"
                     /locus_tag="VQ514_001439"
     CDS             complement(1265..2617)
                     /gene="dnaB"
                     /locus_tag="VQ514_001439"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HET2500187.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            complement(2661..3113)
                     /gene="rplI"
                     /locus_tag="VQ514_001440"
     CDS             complement(2661..3113)
                     /gene="rplI"
                     /locus_tag="VQ514_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HET2500188.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            complement(3110..5089)
                     /locus_tag="VQ514_001441"
     CDS             complement(3110..5089)
                     /locus_tag="VQ514_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HET2500189.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTSLLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDV
                     SREQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMF
                     SRRVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDE
                     IGKVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISD
                     KIRSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFI
                     EKEGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQD
                     FPDNTVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRV
                     TSRTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDTK
                     CYDTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGH
                     FNLAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            complement(5225..5773)
                     /gene="raiA"
                     /locus_tag="VQ514_001442"
     CDS             complement(5225..5773)
                     /gene="raiA"
                     /locus_tag="VQ514_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HET2500190.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNVVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            complement(5853..6515)
                     /locus_tag="VQ514_001443"
     CDS             complement(5853..6515)
                     /locus_tag="VQ514_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HET2500191.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQFWCKEGVGVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            complement(6512..7810)
                     /locus_tag="VQ514_001444"
     CDS             complement(6512..7810)
                     /locus_tag="VQ514_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2500192.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPML
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            7866..8501
                     /locus_tag="VQ514_001445"
     CDS             7866..8501
                     /locus_tag="VQ514_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HET2500193.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITTIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            8786..9706
                     /gene="cysK"
                     /locus_tag="VQ514_001446"
     CDS             8786..9706
                     /gene="cysK"
                     /locus_tag="VQ514_001446"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HET2500194.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKLGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(9903..10301)
                     /locus_tag="VQ514_001447"
     CDS             complement(9903..10301)
                     /locus_tag="VQ514_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2500195.1"
                     /translation="MKSPIPKAPLAHNFGSASIIAHTIHQKFNLKVPNYRQEEDWARM
                     GLPITRKEISNRHIKTSQYYLEPLYNLLRERLLTQPLLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHVLIDLFISYFNPL"
BASE COUNT         3197 a         2282 c         1678 g         3222 t
ORIGIN      
        1 agtatctgat cataatgaac cttctcccca ggataatagt aatcaatcac agttccgcta
       61 actaggggtt cagttttgat agcagacttg gtcttaaaat aatgagttcc acctgttgaa
      121 gaaagaacag aattacctag aggattttta ttcacagcct ccaattgaac ataacgatag
      181 cttccattat aggcagtata actcaaccac ttgtagccgt ctttttcgag aatttgatca
      241 taatgaacct tctctccagg ataatagtaa tcaatcacag ttgcactaac taggggttca
      301 gttttaatag cagacttggt cttaaaataa tgagttccgc ctgttgaagt tgaggattga
      361 ctattcccaa cactgccacc atccaaatct ttaaaatgaa taaatcctgt catcgtgttt
      421 gcttttataa ctcgtttatt ataggattct gtataaccat agttatattc ctcaatctca
      481 atctgatctc ccattacatt tgacacccag gcaacatgac cataagttcc tgcagtagac
      541 caagtaatgg aaccaatcgt cggtgtatta tctacacgat aaccttcccg acgagcacga
      601 tgtccccatt cattcgcatt tccataagct gccggaattt caaaaccatt gacattactc
      661 aaacgaaagg ctacaaaaga agtacactga cgagaataca tgcgccactg atcaatctcc
      721 tggctcccat ttttataata agcaggataa tcatccccac gcgcaatcga tccatttcct
      781 ctggaatagg catagacact atcaccagct gctagcatca atcctactac taaaaaagga
      841 acaagctttt taactgacat cttcaagtaa taacttgttc ctcttgctat aaacggtaaa
      901 attttcattc ttcctccttg aaaaataata taaagcgatg attgccttca cctaaattat
      961 tcggagaaaa aagaaaaagt gagaaaattt ctcactttag tcaagataat gaatcaaatt
     1021 cttttctgta agaatatctg agtaagtaaa ggattcaacg taaacattaa cttgtttatc
     1081 tccttccaca tccccaaact ccacaataaa tagagatgga taaacttcaa ttagcttacc
     1141 caatctattt ttttggcgct tacgaccatt ctccaaagtc atttctacga cttgtccctc
     1201 atgtgccttg atttcttctt tgattttttt catcttggct acatctgtaa atgcatctga
     1261 catcttatgc ctccctcttt gagatacttg aaaatttatt gtattctttt tggaaaatca
     1321 attccactgt tccacgagct ccactacggt ttttctcgat aataacttcc accttattat
     1381 ttgggatacc ctcctcttct tcaccaccac gttcatagta gtcatcgcga tagagaaaag
     1441 ctacgatatc agcgtcctgc tcaatagacc cagattcacg aatatcagac aagaccggtc
     1501 tcttgtcctg acgttgttct acaccacgag aaagctgact cagagcgatt actggaacct
     1561 tcaattcctt ggctagtatt ttcaactgac gagaaatttc agaaacttct tgttgacgat
     1621 tttctcgacc agttcccgtg ataagttgca aatagtctat caaaatcaaa ccaagatttc
     1681 cagtttcttg agccaattta cgagaacgag aacgaatctc tgtaatccga atacctggcg
     1741 tatcatcgat atagatactg gcgttagcta gattaccctg agcaatagta tatttttgcc
     1801 actcctcatc tgtcaattgc cctgtacgga tagaatgtga ctccactaag ccttctgcag
     1861 ctaacatacg atctaccaag ctttccgcac ccatttcgag tgaaaaaata gcaaccgttt
     1921 tgtccaactt agtcccaatg ttctgagcga tattcaaggc aaatgctgtc ttaccaactg
     1981 ctggacgagc tgctaagata atcaactcct cctcatgaag tcctgttgtc atatgatcca
     2041 aatcacgata acctgtcgca atacctgtaa tatcggtcgt ttgttgcgag cgagcttcca
     2101 gatttccaaa gttgagattc aacacatctc gaatgttctt aaacccgctt cgatttgcat
     2161 tttcactgac atcaatcagc cctttttctg cctgagcaat aatttcatca gctggttgtg
     2221 acgcttcgta agcttggttg acagactctg tcaacttggc aattaaacga cgtagcattg
     2281 ctttttctgc aacaatctta gcataatact ccgcattagc agaagttggc acagaattaa
     2341 caatctcaac caagtaagac aagccaccaa tattctgtaa atcaccttga ttatcaagga
     2401 tagtacgaac cgttgttgca tctatggcat caccacgatc ggataaatcg accatggctt
     2461 ggaaaatcaa acgatgggca tacttaaaaa agtcccgaga ctcaatgtat tctcgcacaa
     2521 aaacaagttt actctcatca ataaagatag cccctaaaac ggattgctca gctaagatat
     2581 cttgaggttg tactcgtaac tcttctactt ctgccatcag acttcccttc cttttacaat
     2641 cttgtcaaga aggtgtaaac ttatccttct ttcacacgaa gattgattac acttgtgata
     2701 tcttgataga ttttcactgg cacatcaatc aaaccaaccg ctcgaatcgg agcttgtact
     2761 tgaatatgac gtttatcaat cttaattcca aattgttttt gcaattcttc tgcaatcttc
     2821 ttattggtaa tagagccaaa ggtacgacca tctggaccaa ctttttcaac aaattctaca
     2881 acagtttctt ctgcttcaag ttgtgcttta attgcttttc cttctgcaat catctcagcg
     2941 tgagcttttt cttccgattt ttgtttacca cgaagttcac ctacagcttg agcagtcgct
     3001 tctttggcta gattcttttt gataagaaag ttttgcgcat accctgttgg tacttcctta
     3061 atttcgcctt ttttaccttt tcctttaaca tctgctaaaa agattacttt cattcttctt
     3121 tctccttttc ctttatttca tttaatacaa tttctgtcag tttttcacct gcttctgaca
     3181 aggttacatc tttaatttga gctgctgcca aattaaagtg gcctccaccg cctaactctt
     3241 ccataatccg ttgtacattc agtttactac gacttcgagc tgagatagag ataaatcctt
     3301 gtgtattctt cgcaagaaca aaactcgctt caatacctga catggctaac atggcatctg
     3361 ctgccttact aataacaact gtatcatagc atttcgtgtc cttagcctct gctattagta
     3421 catctgaacc taatttacgc ccctgtaaaa taagttcatt gacctcacga tattcttcaa
     3481 aatctgtcgc agcgatttcc tggatagcaa tactatcact tccgcgcgtt ctgagatagc
     3541 tagcaacatc aaatgtccga ctagttactc gcgaggtgaa atttttagta tccaacatca
     3601 taccagccat caagacactt gcttgcatac gactcaaacg atttttctta gaattctgga
     3661 actgaatcaa ttccgttacc aactcactgg cactacttgc accactttcg atataagtaa
     3721 tgaccgtatt atctggaaaa tcctgatccc ttctatggtg gtcaataaca atggtttggg
     3781 taaataaatc ataaaattct tttgataatg ttaaggctgt ctttgaatgg tctacgagaa
     3841 tcaacaaaga acgattggtc accatcccca ttgcatcctt aacagacaac aacttcgtaa
     3901 ctccttcttt ttctatgaat gaaacagctc gttcaatatc tggagacatt tgttcttcat
     3961 cataaagagc atagctattt tcaatcacat tgctggcgaa caactgcata cctacagcag
     4021 agcccaaagc atccatgtct aaatttttgt gaccgactac aaaaacctga tctacactcc
     4081 gaatcttatc tgaaatagct gtcatcatag cgcgcgtacg agtccgtgta cgcttgattg
     4141 aagcagcaga cccaccacca aaataaactg gatttttcgt ttcgtcgttt tccttaacaa
     4201 ccacctgatc gccaccacgt acttcagcca agttcaaatt gagcaaagca actttcccta
     4261 tctcatcatg atttccatcg ccataagaaa atcccatact taaggtcaag ggcaactgtc
     4321 tctgtttcga ctcttctctg aaagcatcaa taacagaaaa tttatcattc atcaagccct
     4381 caagcaccgt gtagtcagta aatagataaa atcgatccat acttacccga cgagaaaaca
     4441 tcatgtattt ttctgaaaac tctgatataa aattagctac aaaactattg atttgactaa
     4501 tatctgactc agaagttgca tcctccaaat catcataatt atccacagag acaatcccaa
     4561 tcactggtct acttgttacc aattcatctg ttatggcttg ttccctggat acatctacaa
     4621 aatacaaaac accggaagaa gcatccatat gaacagcata acgcttctca ccaagcttgg
     4681 cataagtaga cggatttcct actgaagctg aagccttgat aatcgtttga acagcttcta
     4741 aatcaaaatc accatcttcc ttggtcaaaa tcaattcagc atagggatta aaccactcaa
     4801 cctctccaga agataaattc aatttcataa cacctacagg catttgttcc aatagagatg
     4861 tcaaactttc ttccgcttgg tggtttacat actgtatctg ttctacatca ctccttgtat
     4921 aatgcactct cagtttctta aataaaaaaa catagcctcc tacaaaaaga aacaaaatta
     4981 aaaccgtcaa cagattatta ttaacaaaaa taatgaaagt ggataagact ccaaacgcaa
     5041 tcaatcctac tagaatagga aaaattggac ttacataaaa ttttttcatt caaaacctct
     5101 tggcacccat tataccataa tacccctcaa aaagcgactt tttaaaagtg taatcagtaa
     5161 ttctatcaat tataagaaaa aggtagttta caattcagta aacctacctt tacacatatt
     5221 gaaattaaga ttctttaacc tctaacaaac caatttcgcc atcctcacga cgataaatca
     5281 cattggttgt ctgatcttca acatccacat agataaagaa atcatgcccc aataaatcca
     5341 tttgtagaat tgcttcttcc aaatccattg gttttaaatc aatttgtttt gaacgaacaa
     5401 ctttagactg gacaacattt gaatcttcca ccaaagcatc tgtaaataat tgaccagttg
     5461 ctaccttatt tttattttta cgctcgattt ttgttttatt tttacgaatc tgacgttcaa
     5521 ttttatcagt tacaaggtca attgaaccat acatatcttg agatacatct tctgcgcgga
     5581 gagtaataga tccaagcgga atcgttactt ccactttagc cgttttttca cgataaactt
     5641 ttaagttaat tcgggcatcc aactcttgtt ctggttggaa gtacttttcg atcttttcga
     5701 gtttagaaac tacataatca cgaattgctt ctgttacttc taggttttca ccacggatac
     5761 tatatttaat catatgagta ccttctttct aaacattttt gtttttatga ttttattata
     5821 acgctttcat tctatttttg caaatttttt cctcatctta caagggaaaa tgtttttaca
     5881 tccttagcac cagcttcttc caacagtttc ttaacacgat ttatagttgc tcctgtagta
     5941 tagatatcat ctataagtag gattttttta ggaatagtga ctccactttt aataaagaaa
     6001 ggaagttctg tccccaagcg ctctgaacga tttttagaag aactggctct ctcttctctt
     6061 ttctctaata aatccagata ctcaaagcct gctgcctcta ccaagccctc aacctgatta
     6121 aatcctctat tagcatatct atcaggactt aggggaatta caacaaattg atactctttg
     6181 tactttttca actcctcact taaaaatgaa gcgaaaactt ttcttaacag gaagtctcca
     6241 tcaaacttat accgactgaa aaaatccttc atagcttgat tgtaagtaaa aatcgctcta
     6301 tgactgactc caactccttc tttacaccaa aattgacaat cttgacactt tgttgacaac
     6361 tctgttttca tacaatttgg acagttctct tccccaattc tttcaaaagt agaatcacag
     6421 tctgaacaaa gacaagagtc atcattcctc agaagtaaga gactactaaa agttaaaaca
     6481 gtcttcatag tctgcccaca taacaagcac ttcatagacc agcctcctta ttcatcatct
     6541 gaatttcctt aatcgccttc ttgattgaag catttaaccc atcatggaag aaaagcaaat
     6601 ctcctgtcgg tctatccatg cttcgtccaa ctcgtccacc aatctgaatc aaactagact
     6661 tggtaaacaa acgatgattg gcttctacta cgaaaacatc cacacaaggg aaggtaactc
     6721 cacgctccaa gattgtcgta ctgataagta ttgtcagttc tccatctcga aaagcttgta
     6781 cttgctctaa tcgatcctct gttacagaag atacaaagcc aattttctca tttggaaatt
     6841 gctcctgtaa gatttcttct aactgctccc ctttcttaat ttctgaagca aaaatgagta
     6901 acggataagc tgtctttctc tgcttctcaa tataggactt taactttggt gacaaacgat
     6961 tcttgtctaa gtagcgatta aaatccgata accaaattgg ttttggaata atcaacggat
     7021 ttccatgaaa ccgtctcggt aaattcagtc tttttagttc tcctaaacgg acctttttat
     7081 ctaactcatt ggtcgaagtc gctgttaaaa agattctcaa tccattctcc tttacactat
     7141 tcttgacagc gtggtaaagc attggattat caacataagg aaaagcatct acttcatcca
     7201 ctatcagcaa atcaaaagct tgataaaact tcaataactg atgggttgtt gcaacaacta
     7261 gtggtgttcg aaaataaggt tccgattctc catgtagcaa agctatcccg caagaaaaat
     7321 cctgttgcag gcgcttgtac agctccaaac aaacatctat gcgaggacta gccaaacaca
     7381 ctgcaccacc cgcattgatc actttagcca ctacttgata aatcatttct gtctttccag
     7441 ctcctgttac cgcatgaact aaggttggct tttgcttgtc tactgcttga agcagtccct
     7501 ctgacacctt ctcttgaaaa ggagttaatt ggccgcgcca tttgagaaca tcttgcttcg
     7561 gaaaatcctc ctgcggaaaa tagtataaag tttgatcact tctgactcgc ttcatcagca
     7621 agcactctcg acaatagtaa gcaccgatgg gcaaatacca ttcttctaga atagtactat
     7681 tacaacgttg acagaaaagt ttccccttct cctttctcat tgctggaagt ttctccgcca
     7741 actgacgttc ttcttctgtt aattcattct cagtaaataa acgaccgaga taatctaaat
     7801 ttactttcat acttctttat tcgtaaaaac tagcacttta gatgattttt tagtacaatt
     7861 aaatcatgga atttaggaca attaaagagg acggccaagt ccaagaagaa atcaaaaaat
     7921 ctcgctttat ctgccatgcc aagcgtgttt atagcgaaga agaggctcgt gacttcatta
     7981 ctaccatcaa aaaagaacac tacaaagcga cacataactg ctctgccttt attattggag
     8041 aacgtagtga aattaaacgt acaagtgatg atggtgagcc tagtggtact gctggtgttc
     8101 ccatgcttgg ggtactagaa aatcacaatc tcaccaatgt ctgtgtggtc gtgacacgct
     8161 actttggtgg tattaaacta ggcgctggag gactaattcg tgcttacgcc ggcagtgtcg
     8221 ccttagctgt caaagaaatt ggtattattg aaataaaaga acaggctggc attgctattc
     8281 aaatgtctta tgctcagtac caagagtaca gtaacttcct taaagaacat gatctcatgg
     8341 agctggatac aaactttaca gatcaagttg atacgatgat ttatgttgat aaagaagaaa
     8401 aagaaactat taaagctgca cttgtggagt tttttaatgg aaaagtcact ttaactgacc
     8461 aaggtttacg agaggttgaa gttcctgtaa acttagtgta aacaatgaat aatacagcgt
     8521 ttcgttgaca ttctcacaac tactttagcg agcaaaataa aaagatgcgt accaaaatat
     8581 actagaaaat gaagcaattc aaacgaaacc tgatatcgtt ttccttcaca cctatttact
     8641 agaattaact gaacgcaatc acttgaaaat taatgacttt gatctatgat atatagaaat
     8701 cctatcgctt cgataggatt tctatatttt acaaatggta tggatagcgt tatactaaag
     8761 atatcttata caaagaggta ttcatatgtc tatttataac aacattactg aattaatcgg
     8821 tcaaacaccg attgttaaac ttaacaacat cgtgccagaa ggtgctgcag acgtctatat
     8881 aaagcttgaa gcatttaatc ctggttcatc tgtaaaagac cgtattgccc ttagcatgat
     8941 tgaaaaagct gaacaagatg gtattctgaa acttggttct actattgttg aagcaacaag
     9001 tggaaacacc ggtattggac tttcatgggt aggtgctgct aaagggtata aagtcgtcat
     9061 cgttatgcct gaaactatga gtgtagaacg acgtaaaatt atccaagctt atggtgctga
     9121 actcgtccta actcctggta gcgagggaat gaaaggtgct attgctaagg ctcaagaaat
     9181 cgctgctgaa cgtgatggtt tccttcctct tcaatttgac aatccagcta atccagaagt
     9241 acacgaaaga acaacaggag ctgagatact agctgctttc ggtaaagatg gattagatgc
     9301 ctttgttgct ggagtaggta ctggtggaac gatttctggt gtttctcatg cactcaaatc
     9361 agaaaattct aacattcaag tttttgcagt agaagcagat gaatctgcta ttctatctgg
     9421 tgaaaaacct ggtcctcaca aaattcaagg tatctcagct ggatttattc ctgatacact
     9481 tgatactaaa gcctatgatg gtatcgttcg tgtaacatca gatgacgctc ttgcactcgg
     9541 acgtgaaatt ggtggaaaag aaggcttcct tgtagggatt tcctcagctg cagctatcta
     9601 cggagccatc gaggttgcca aaaaattagg tacaggtaaa aaagtccttg ccctagcacc
     9661 agataacggt gaacgttatc tctctacagc actttatgaa ttgtaaccgt ccaataacga
     9721 agtctattga aaaatctcca gactagagaa ctcacggata gttcctaatc tggagatttc
     9781 ttatttgcac ttttcttgta caactttagt ccatggtaaa taggcctcta aaacctcttt
     9841 gtttacgaga gtttccacgt ttggaagaca ttctagaaga taggatagat atttctcact
     9901 atttataatg gattgaaata agatatgaac aaatcgatta ggacatgatg gtaaagcgta
     9961 atcccttgtt tctcagcttt cccagacaaa aaagtccaat agtaagtcag ctgactatca
    10021 ctctctagca ccctataaga agtttcatcc gcatgaagta agggctgagt caatagtctc
    10081 tctcgcaaga ggttataaag gggctccaaa tagtattgac tcgtcttgat atgccgatta
    10141 gagatttcct tacgtgtgat tggtaaaccc atcctagccc aatcttcttc ttggcgataa
    10201 ttgggtacct tcagattaaa cttctgatgg atggtgtgag cgataataga agctgagcca
    10261 aagttatgcg ctaaaggggc tttaggaata ggagatttca caagcttatc cagatgatta
    10321 tcttttactc gttatggaca atgctatatg gcataaatca agtaccttaa agattccga
//
LOCUS       DAVBCW010000068        10314 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVBCW010000068 DAVBCW010000000
VERSION     DAVBCW010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10314
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.068"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            87..1262
                     /gene="mutY"
                     /locus_tag="VQ514_001448"
     CDS             87..1262
                     /gene="mutY"
                     /locus_tag="VQ514_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HET2500196.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     LIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            1317..2021
                     /locus_tag="VQ514_001449"
     CDS             1317..2021
                     /locus_tag="VQ514_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2500197.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            2014..3363
                     /gene="vicK"
                     /locus_tag="VQ514_001450"
     CDS             2014..3363
                     /gene="vicK"
                     /locus_tag="VQ514_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HET2500198.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            3365..4174
                     /locus_tag="VQ514_001451"
     CDS             3365..4174
                     /locus_tag="VQ514_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET2500199.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            4265..4507
                     /locus_tag="VQ514_001452"
     CDS             4265..4507
                     /locus_tag="VQ514_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HET2500200.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            4509..4763
                     /locus_tag="VQ514_001453"
     CDS             4509..4763
                     /locus_tag="VQ514_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HET2500201.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            4810..5583
                     /locus_tag="VQ514_001454"
     CDS             4810..5583
                     /locus_tag="VQ514_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HET2500202.1"
                     /translation="MKKKWFFADYYDTTIILLALISVILVLLGFAEMIDLDNPPYSII
                     DLVIWGVFVIDYSWRFFTTKRKWRFILENIFDLLAILPLNAIFTVFRLGRIFRLAKLT
                     KLLKLTRLLRIIGLTGKLERKISRFLRTNGLIYILYVNIFIVLVGSSILSVVEEKSFS
                     DSLWWALVTVTTVGYGDIVPASIFGKWLAVLLMLVGIGTIGMLTSALTNFFVKDNPDE
                     QIKLDKLKDELSSQRILLEKQSKKIEELHKMIQDLIEKT"
     gene            complement(5843..6829)
                     /locus_tag="VQ514_001455"
     CDS             complement(5843..6829)
                     /locus_tag="VQ514_001455"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HET2500203.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGLYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            7023..9491
                     /gene="gyrA"
                     /locus_tag="VQ514_001456"
     CDS             7023..9491
                     /gene="gyrA"
                     /locus_tag="VQ514_001456"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HET2500204.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIALRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMIGQVLSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEE"
     gene            9491..10234
                     /gene="srtA"
                     /locus_tag="VQ514_001457"
     CDS             9491..10234
                     /gene="srtA"
                     /locus_tag="VQ514_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HET2500205.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSDEILTAFNQPYKQFY"
BASE COUNT         3203 a         1751 c         2301 g         3059 t
ORIGIN      
        1 tagatagaaa agttatatac tatctattga aaatattata aatcagatac aaagacaggt
       61 gaaaccttct gtgttatact agagatatgt tagatttgaa agaatacggt atcgtcatgt
      121 ggccggagga gaaagtcatt tctttccgtg agaaacttct cgcttggtat gatgaaaaca
      181 aaagagattt gccttggagg agaagtaaaa atccttatca catctgggta tctgaaatca
      241 tgcttcagca gaccagggtg gatacagtta tcccttacta cgaaagattc ctggactggt
      301 ttccaactgt cgaaagtctg gcaactgcgc ctgaggagag tttactgaaa gcttgggagg
      361 gcttgggcta ttattctcga gttcgcaata tgcaggctgc agcccagcag attatgactg
      421 actttggtgg ccaatttcca aatacctatg aaggaatttc cagcttgaaa gggattggac
      481 cttacacagc aggagccatt tccagtattg cttttaactt gcctgagcca gctgtagatg
      541 gtaatgtcat gcgggtcttg gcgcgtctgt ttgaagtcaa ccacgatatt gggattccaa
      601 gtaatcgaaa aatttttcag gcaatgatgg agatcttgat taatccggat cgtccgggtg
      661 attttaatca agccttgatg gacttagggt ctgatattga gtctcctgta aatcccagac
      721 ctgaagaaag cccagtcaag gactttagtg cggcatatca gaatggaacc atggaccgtt
      781 atccaattaa atctcctaag aaaaagccgg ttcctattta tcttaaagca ttagttgtga
      841 aaaattctca aggacaattt ttacttgaaa aaaatgaaag cgaaaagctc ttggcaggtt
      901 tttggcattt cccccttata gaagttgata acttttcgca agaagagcag tttgacctct
      961 ttcatcaggt tgcggaagaa agtgtgaact ttggccccag tccagaagag agtttccagc
     1021 aggactatga cctagatgtt gattggcttg atgtttgttt tgatacagtc cagcatgttt
     1081 ttagtcatcg aaaatggcat gtacagattg tagcaggtca ggtgagtgac ttccatgatt
     1141 tttcagatag ggaagttcgt tggctttcac cagaagaatt caagaattac ccacttgcca
     1201 aaccccaaca aaaaatctgg caggcttatg cacaagccaa cttagacagt agccaagact
     1261 agctattgtg tcttctgact attttttggt ataatagtag agaaaaaggt gaacatatga
     1321 aaaaaatact aattgtagat gatgagaaac caatctcgga tattatcaag tttaatatga
     1381 ccaaggaagg ttacgaagtt gtaactgctt ttaatggtcg tgaagcgcta gagcaatttg
     1441 aagcagagca accagatatt attattctgg atttgatgct tccagaaatt gatggtttag
     1501 aagttgctaa gaccattcgt aagacaagca gtgtgcccat tcttatgctt tcagccaaag
     1561 atagtgaatt tgataaggtt atcggtttgg aacttggggc agatgactat gtaacgaaac
     1621 ccttctccaa tcgtgagttg caggcgcgtg ttaaagctct tctgcgtcgt tctcaaccta
     1681 tgccagtaga tggtcaggaa gcagatagta aacctcaacc tatccaaatt ggggatttag
     1741 aaattgttcc agacgcctac gtggctaaaa aatatggcga agaactagac ttaacccatc
     1801 gtgaatttga gcttttgtat catttagcat cgcatacagg tcaagtcatc acgcgcgaac
     1861 acttgcttga gactgtctgg ggttatgact attttggtga tgtccgtaca gttgatgtga
     1921 ctgtacgacg tctgcgtgag aagattgaag atacgcccag ccgaccagag tatatcttga
     1981 cgcgccgtgg tgtagggtat tacatgagaa ataatgcttg atttactgaa acaaaccatt
     2041 tttaccagag attttatctt tatcctgatt ttgttaggtt tcatccttgt tgtgaccctc
     2101 ttattactgg aaaatagacg tgataatatt cagttgaagc aaatcaatca aaaggttaaa
     2161 gatttgattg caggagatta ttccaaggtt cttgatatgc aaggtgggtc tgaaatcacc
     2221 aatattacca ataatttgaa tgacttgtcg gaggttattc gtctcactca ggaaaatcta
     2281 gaacaagaga gtaagaggct aaatagtatt ctgttttata tgacagatgg ggttcttgcg
     2341 accaaccgtc ggggtcagat tatcatgatt aacgatacag ccaagaagca actggggttg
     2401 gttaaggaag atgttctgaa tagaagcatt ttggaattgc tcaagataga agaaaactat
     2461 gaattgcgtg atttgattac ccaaagtcca gaattgttgc tagattccca agatatcaat
     2521 ggcgaatatt tgaaccttcg agttcgcttt gccttgatac gtcgagagtc tggctttatt
     2581 tcaggtttgg tggctgtttt gcatgatacg acggagcagg agaaggaaga acgcgaacga
     2641 agactctttg tttccaatgt tagccatgag ttacggactc ctctgactag cgtaaaatcc
     2701 tatcttgaag ccttggatga gggggctttg tgtgaaactg tagcaccaga ctttatcaag
     2761 gtttctcttg atgagaccaa ccgtatgatg cgcatggtga cggatctcct ccatctttca
     2821 cgtattgata atgctaccag tcacctagat gtggaactga ttaacttcac tgcttttatt
     2881 acctttatcc tcaatcgttt tgacaagatg aaaggacagg aaaaggagaa aaaatatgag
     2941 ttggtgagag attatcccat caattctatc tggatggaaa ttgatacaga taagatgacg
     3001 caggttgtcg acaatatttt aaataatgct attaagtatt cgccagatgg gggtaaaatc
     3061 actgtcagaa tgaagacaac tgaagaccag atgattttat ccatttctga ccacggtttg
     3121 gggattccta agcaggattt accacgtatc tttgaccgtt tctatcgtgt ggatcgtgct
     3181 agaagtcgtg cacaaggtgg tacaggtcta ggactgtcta tcgctaaaga aattatcaaa
     3241 caacataagg gctttatttg ggccaagagt gaatacggca agggttcaac ctttaccatt
     3301 gtgctccctt atgataagga tgcagtgaaa gaagaagtat gggaggatga agtagaagac
     3361 tagaatgagt gaaataggct ttaaatacag tattttagcg tcgggttcca gtggaaattc
     3421 tttttatctg gaaacctcaa aaaagaagct tttagtagat gcaggcttgt ctggcaagaa
     3481 aattaccagt ctgctagctg aaattaaccg taagccagaa gacctggatg ccatcttgat
     3541 tacccatgag cattcagatc atatccatgg agtaggcgtt ttggctcgca agtatggtat
     3601 ggatctttat gccaatgaaa agacctggca agctatggaa aatagtaaat atcttggcaa
     3661 ggtggattct tcgcaaaagc atatttttga aatgggtaaa accaaaacct ttggagatat
     3721 cgacatcgag agttttggtg taagccatga tgcagtcgca ccgcagttct atcgctttat
     3781 gaaggatgat aagagttttg tcctcttgac agatacaggt tatgtcagtg accgtatggc
     3841 gggcattgtc gaaaatgcgg atggttatct tatcgaggcc aaccatgatg tagagatttt
     3901 gcgatcaggt tcttacgctt ggcgcctcaa acaacgaatc ctatctgacc ttggtcacct
     3961 ttctaacgag gacggtgctg aagctatgat tcggacgcta ggaaatcgta ctaagaagat
     4021 ttaccttggg catttatcta aggaaaacaa tatcaaggaa ctggctcata tgaccatggt
     4081 caatcagctg gctcaagctg atctgggagt cggagtagac tttaaggttt atgatacctc
     4141 accagatacc gcaacaccat tgacagagat atagaaagaa cgctgagaag gtgttctttt
     4201 tatattgact ggacacctaa aaagtaatac aatggtgtta ccattaaaaa agggagcaca
     4261 aaagatgact actattacat taaaagtttc tgaagctgat aaaacattta tgaaagcaat
     4321 ggctaagttt gaaggagttt ccctttcgga acttattcgc accaaaactc ttgaagccct
     4381 agaagatgaa tacgatgctc gtgtggcaga tttagcctat caagagtatt tagaagactt
     4441 ggaaaaagga gttgaaccca ttacttggga agaaatgatg catgatttag gcttgaagga
     4501 tgaataattt gtataaatta gttccaacaa gacgttttat caagcaattg aaaaaattgg
     4561 accgttatac gcagaagcta attacaaact atttacaaac caatgttttg gaagatccaa
     4621 gacgacacgg aaaggctttg gttggtaatc gcgttggtca atggcgctat agaattggta
     4681 attatcgagt tatcgtacaa attgtagatg atgaattagt tgttgctact ctagaagttg
     4741 gtcatcggag agatatttat taaattaaag ctgagtaata acagtataga aagtaaggag
     4801 agtaaaaatt tgaagaaaaa atggtttttt gcagattatt atgacacaac aattatttta
     4861 cttgctttaa tctctgttat tctggtatta cttggatttg ctgagatgat tgatttggac
     4921 aatccgcctt atagtattat tgatttagta atctggggtg tttttgtaat tgattatagt
     4981 tggcgctttt ttacgactaa aagaaaatgg cgctttattc ttgaaaatat ttttgattta
     5041 ttggctattc tccctttaaa tgctattttt acagtgtttc gattagggcg tatctttcgt
     5101 ttagctaaac taacaaaatt actgaaactg actcgtttac ttagaataat tggacttaca
     5161 ggtaaattag aaagaaaaat tagtagattc ttacggacaa atggcctgat ttatatcttg
     5221 tatgttaata tctttattgt tctagtaggg agttcgattt tatccgttgt tgaagaaaaa
     5281 tccttctcag atagtctttg gtgggctctt gtgacagtaa ctactgttgg atatggggat
     5341 attgttcctg cttccatttt tgggaaatgg ctagcagttt tactgatgct tgtcggtatt
     5401 ggaacaatag ggatgttaac aagtgctttg acgaactttt ttgtaaagga taatccagat
     5461 gaacagataa aacttgataa actcaaagat gaattatcga gccaaagaat attactagag
     5521 aaacaatcta aaaagataga agaactacat aaaatgatac aagatttgat tgaaaaaaca
     5581 taataaaaat aaagatgaga caaagtttga gctgaggaga aatcaagtaa gttttgacac
     5641 aatagaacgc atattatcag gtataaaaac ttgatactat gcgttttata gagaaataaa
     5701 ataaaatctg aacaaatcaa ttaggaattt aaagcatttt ataacaacgt tttagagtga
     5761 tggggtgcta tttcaacttc aacctactat aatacagaaa aaaacaactc cctgatgatt
     5821 caaggagttg tctatagtta aattagtttt tagaagcttc ttggaattct ggatttttcc
     5881 atgcttcgtc aataatagct tgcaattctt tagcagatgc ttgcattttt tgagtttctg
     5941 catcgttcaa tggaatgttt actggacgaa cgataccatg tgcaccaaca acagctggtt
     6001 gaccgataaa gacattctca actccgtata gaccttcttg gaatactgaa agtggaagta
     6061 ctgcgttttc atcgtcaagg attgctttag tgatacgagc aagggctact gcgataccgt
     6121 agtatgttgc accttttttg ttgatgattg tgtaggctgc atcacgaaca ccttcgaaca
     6181 attcaatcaa ttcagcttct tgaacatttt gagtgtcttt aaggaattct tcaaggttta
     6241 caccagcgat gttagcgtgt gaccaaacag cgaactcaga gtcaccgtgt tcacccatga
     6301 tgtaggcgtg cactgaacga gcatccacat ccaatttttc agcaagtgct tgacggaaac
     6361 gagctgagtc aagtgaagta cctgaaccga taacgcgttc tttagggaaa ccagagaatt
     6421 tccaagttga gtaagtcaaa acgtcaactg ggttagcagc aacaaggaag atacctttga
     6481 aaccagattc aacaacttga gttacgattg atttgttgat agcaaggttt ttacctacaa
     6541 ggtcaagacg agtttcacct ggtttttgag gtgcacctgc agtaatcaca acaaggtcag
     6601 cgtctgcaca gtcagagtat tgagctgcat agattttttt aggtgaagtg aaggcaaggg
     6661 cgtgactaag gtcaagcgca tcaccaacag ctttttcatg caattgtgga atttcgataa
     6721 ttccaagctc ttgtgcaatt ccttggttaa caagtgcaaa agcgtaagat gaacctacag
     6781 caccatcacc gacaaggata acttttttgt gttgtttagt tgaagtcatt gttttaaaca
     6841 tctccttaat tttattaggg gattttccct agacaacttc attctatcac ttttaaaaaa
     6901 ctttgtcacg aatatgcctt atagttctcg atgtaaacgt tttagtggtt tagaggctga
     6961 aatagatggg aatttatggt ataatgttgt tacttactaa ttgtgaaatg aggcatttat
     7021 taatgcagga taaaaattta gtgaatgtca atctgacaaa ggagatgaag gcaagtttta
     7081 tcgactacgc catgagtgtt atcgtagcgc gagctcttcc tgatgttcga gatggcttaa
     7141 aacctgttca ccgtcgcatt ctctacggaa tgaatgaatt gggtgtgacc ccagacaaac
     7201 cccataaaaa atctgctcgt attacagggg atgtcatggg taaatatcac ccacacgggg
     7261 attcctctat ttatgaagcc atggtccgta tggctcaatg gtggagctac cgttacatgc
     7321 ttgtagatgg tcatgggaat tttggttcca tggatggaga tagtgctgcc gctcaacgtt
     7381 ataccgaggc acgtatgagc aagattgctc tggaaatgct tcgtgatatc aacaaaaata
     7441 cagttgattt cgttgataac tatgatgcca atgaacggga acccttggtc ttgccagcgc
     7501 gttttccaaa ccttttggtt aatggagcaa ctggtatcgc ggttgggatg gcaaccaata
     7561 ttccacctca taatctgggt gaaaccattg atgcagtgaa gttggtcatg gataatcctg
     7621 aagtgactac caaggacttg atggaagtct tgcctggacc agattttcca actggtgctc
     7681 ttgtcatggg gaaatcaggt atccataagg cttatgaaac aggtaaaggt tcgattgccc
     7741 tacgttctcg tacagagatt gaaacgacta agactggtcg tgagcgcatc gttgtaacag
     7801 aatttcctta catggtcaat aaaaccaagg tgcatgagca tattgttcgc ttggttcagg
     7861 aaaaacgcat tgagggtatc acagcagtac gtgatgagtc aaaccgtgaa ggtgttcgat
     7921 ttgttattga agtcaagcgc gacgcctcag ccaatgttat tctcaataac ctcttcaaaa
     7981 tgacccaaat gcaaaccaat tttggtttca atatgctcgc tatccaaaat ggtataccga
     8041 aaattttgtc tcttcgtcag attttggatg cttatatcga gcaccaaaaa gaagtggttg
     8101 ttcgtcgtac acgttttgat aaggaaaaag cggaagcgcg cgctcatatc ttagaaggtc
     8161 tcttgattgc gctagaccat atcgacgaag tgattcgtat catccgtgct agtgaaacgg
     8221 atgcggaagc tcaagctgag ttgatgagca agtttaagct ttctgaacgt caaagtcaag
     8281 ctatccttga tatgcgtctt cgtcgtttga caggtttgga acgcgataag attcaatctg
     8341 agtatgatga cctcttggct ctgattgcgg atttagcaga tattcttgct aagcctgaac
     8401 gtgtttctca aattatcaaa gacgaattgg atgaagttaa acgtaaattt tctgataagc
     8461 gccgtacaga gttgatgatt ggacaggtct tgagtctcga ggatgaggac ttgattgaag
     8521 aatcggatgt cttgattacc ctttctaaca gaggctacat taagcgtttg gatcaggacg
     8581 agttcactgc tcaaaaacgt gggggtcgtg gtgtccaagg aacgggagtg aaagatgatg
     8641 actttgttcg tgagttagtg tcaactagca cccatgatca tctgctcttc ttcacaaaca
     8701 agggacgtgt ctatcgtctt aaaggttatg aaattcctga gtatggtcgg actgccaaag
     8761 ggctaccagt agtcaatctc ttgaaattgg atgaagacga aagtattcag acggttatca
     8821 atgttgagtc tgatcgcagt gatgatgctt atctcttctt tacaacccgt cacggtattg
     8881 tgaagagaac cagtgttaag gagtttgcca atattcgtca aaatggtctc aaagcgctga
     8941 atttaaagga tgaagatgag ttaatcaatg tcttgttggc agaaggagat atggatatta
     9001 tcattggtac caagtttggt tatgcagttc gctttaatca atcagccgtt cgtggtatga
     9061 gtcgtatcgc cactggtgtg aaaggtgtta accttcgtga aggagacaca gttgttggtg
     9121 ccagcttgat tactgatcaa gatgaggttc ttattatcac agaaaaagga tatggtaagc
     9181 gtacagtcgc tactgaatac ccaacaaaag gtcgtggtgg taagggaatg cagacagcta
     9241 aaattaccga aaaaaatggc ttgctggccg gtcttatgac tgttcaaggg gatgaggatt
     9301 tgatgattat cactgataca ggtgtcatga ttcgaaccaa tcttgccaat atttcacaaa
     9361 caggacgtgc aactatggga gttaaagtaa tgcgcctgga tcaagatgct cagatagtga
     9421 ctttcacaac ggttgcggtg gcagaaaaag aagaagttgg gacagaaaac gaaacagaag
     9481 gtgaagaata atgtctcaaa aaaataataa aaagaaaaac aagcgaaaaa atctgctgac
     9541 aaatatccta gcaggatttc tgatattact gtcactggct ttgattttta atactcaaat
     9601 tcgaaatatt ttcatagtct ggaataccaa taagtatcaa gttagccagg tatcaaaaga
     9661 aaaattagaa gaaaatcagg atacagaagg caattttgac tttgattctg tcaaagctat
     9721 ctcttcggaa gctgttctaa cttctcaatg ggatgctcaa aaattaccag ttattggggg
     9781 aattgcaatt cctgaattgg aaatgaattt gccgattttt aaaggacttg ataatgttaa
     9841 tctcttctac ggagctggta caatgaaacg cgagcaagta atgggagaag gaaattatag
     9901 tctagctagt caccatatct ttggtgttga taatgctaat aaaatgttat tttctccttt
     9961 agataatgct aaaaatggca tgaagattta tctaaccgat aaaaataaag tttatactta
    10021 tgaaatacgt gaagtcaaac gtgtgacacc ggatcgtgtt gatgaagttg atgatagaga
    10081 tggggtcaat gaaatcacat tagtaacctg tgaagacctt gctgctacag aacgtattat
    10141 tgtcaaaggt gatttgaaag aaacaaaaga ttattcacaa acatctgatg aaattctaac
    10201 agctttcaat caaccatata aacaatttta ttaatacaaa tcagtgaaat catggatttc
    10261 actgattttt taagattttc ataaaaatat agtaaaatga aataagaata ggac
//
LOCUS       DAVBCW010000069        10152 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVBCW010000069 DAVBCW010000000
VERSION     DAVBCW010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10152
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.069"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            118..1560
                     /gene="gtfA"
                     /locus_tag="VQ514_001458"
     CDS             118..1560
                     /gene="gtfA"
                     /locus_tag="VQ514_001458"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HET2500206.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGEKYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKDLFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTEILPEIHEHYSIQFKIADHDYYVYDFTLPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAREVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVAEIDLQNQTYRVIENGVEVSF"
     gene            1698..2891
                     /locus_tag="VQ514_001459"
     CDS             1698..2891
                     /locus_tag="VQ514_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET2500207.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            3578..5557
                     /locus_tag="VQ514_001460"
     CDS             3578..5557
                     /locus_tag="VQ514_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2500208.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            5624..7120
                     /locus_tag="VQ514_001461"
     CDS             5624..7120
                     /locus_tag="VQ514_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2500209.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGALTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPGTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            7120..8046
                     /locus_tag="VQ514_001462"
     CDS             7120..8046
                     /locus_tag="VQ514_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2500210.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSIRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            8055..9122
                     /locus_tag="VQ514_001463"
     CDS             8055..9122
                     /locus_tag="VQ514_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2500211.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            9133..10059
                     /locus_tag="VQ514_001464"
     CDS             9133..10059
                     /locus_tag="VQ514_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2500212.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
BASE COUNT         2990 a         1802 c         2127 g         3233 t
ORIGIN      
        1 ctacggtaag gcgacactga cgtggtttga agagattttc gaagagtata acatgaattt
       61 gatttacgaa gccagatcat atgagacttg gcttcaatta gaaaaaggag agtaatgatg
      121 ccaattcaaa ataaaaccat gttgattacc tattctgata gccttggaaa taatcttaaa
      181 gacttatatg ataatttgga agagcatttt ggagatgcta ttggaggagt tcacctttta
      241 ccatttttcc catcaacagg tgatcgtgga tttgcgccag ttgactacga cgaagtggac
      301 tcagcttttg gtgattggga ggatgttaag cgtttaggtg agaaatatta tcttatgttt
      361 gattttatga ttaatcatat ttctcgtcaa tccaagtatt ataaggacta tcaagaaaaa
      421 catgaagcca gtgaatttaa agatctcttt ttaaactggg ataagttttg gccagaaaac
      481 cgtccgacac agtctgatgt agatttaatt tacaagcgta aggatcgtgc accaaagcaa
      541 gagattgtgt ttgaagatgg ttcagtggaa catttgtgga atacctttgg tgaggagcag
      601 attgatcttg atgtgaccaa agaagtaact atggaattta tccgtaagac cattcagcac
      661 ttggcaagta atgggtgtga tttgattcgt ctagatgcct ttgcttatgc agtgaagaaa
      721 ttggatacta atgatttctt tgtggaacca gatatttggg atttattgga caaagttcga
      781 gatatcgctg ctgagtatgg gacagagatt ttacctgaga ttcatgaaca ctattcgatt
      841 cagtttaaaa tagcagacca tgattactat gtttatgatt ttactcttcc aatggtgaca
      901 ctttatactc tttacagttc cagaacagag cgtttggcta agtggttgaa gatgagcccg
      961 atgaagcaat ttacgacgct agatacccat gatgggattg gagtagtaga tgtcaaggat
     1021 atcctgaccg atgaggagat tgactatgct tcaaatgaac tctataaggt tggagccaat
     1081 gtcaaacgta agtactctag tgccgagtat aacaacttag atatctacca aatcaattca
     1141 acctactatt cagcgcttgg agatgatgat gtcaagtatt ttctcgctcg tctaattcaa
     1201 gcttttgccc caggtattcc tcaggtttac tatgtgggtc tattagcagg caagaatgac
     1261 ttgaaattat tagaagaaac taaagaaggt cgaaatatta atcgtcatta ctatagcaac
     1321 gaggaaatag caagagaagt gcaacgacct gttgtgaagg cccttctcaa tctattttct
     1381 ttccgtaacc gttcagaagc ctttgatcta gaagggacta ctgagataga gacaccaaca
     1441 gctcacagca ttgtaatcaa acgtcaaaat aaagataagt ccgtaacagc agtagcagaa
     1501 attgatttgc aaaatcagac ttatcgggta attgagaatg gagttgaagt atcattttga
     1561 agccttgata tggtggataa aatagggttt ttattttgga aaacgtttcc tttgttttca
     1621 aattgctaaa aaagtggtac aatataggat agcttactat tatctgaatc agctgatttg
     1681 gagagaaagg attcattttg aaatcaatag gctttattga aaagctgaag gggttgtcta
     1741 gtaaagagct gattttattg ggaattatcc taagtatctt tttacccttt tatctttttg
     1801 tagttgtact ctgtttatat attatcagtt tgatttttac aggagacatg aaaagtattc
     1861 ttcagaaaat gggggagcat ccgatgctgc ttctttttct tagctatagt actgttatat
     1921 ccattcttgc acaaaattgg atgggtcttg tggcttcagt aggaatgttt ctatttacta
     1981 ttttcttttt gcactatcag tcgattttat cccataaatt ctttcgattg attttgcagt
     2041 tcgtcttgtt tggtagtgtc ttgtcagctg cttttgccag tttagaacat ttccaaattg
     2101 tgaagaaatt taactatgct tttctttcac ccaatatgca ggtgtggcat cagaaccggg
     2161 cagaagtgac cttctttaat cctaattatt atggaattat ttgttgtttc tgtattatga
     2221 ttgctttcta tctgtttaca acgaccaagt tgaattggtt gaaagtattc tgtgtgattg
     2281 caggctttgt taatctcttt ggtttgaact ttactcaaaa tcgaactgcc tttcctgcta
     2341 ttatcgctgg agcaattatc tatctcttta cgactattaa aaactggaag gccttttggc
     2401 ttagtattgg ggtcttcgcg attggtttga gtttcctctt ttctagtgat ttgggagttc
     2461 gaatgggtac tttagactct tctatggaag aacgcatttc tatctgggat gctgggatgg
     2521 ccttgtttaa gcaaaatcct ttttggggtg aagggccatt gacctatatg cactcttatc
     2581 ctcggataca tgctccttat catgaacatg cccacagtct ttatattgat acgattctga
     2641 gttacggaat tgtgggtacc attttattag ttttgtcttc tgttgctcct gttcgcttga
     2701 tgatggatat gagtcaggag tcggggaaac gtccgattat cggcctttat ctatctttcc
     2761 ttacagtggt tgctgtgcac ggaatttttg acttggctct cttctggatt cagtcaggct
     2821 ttattttctt gctagttatg tgcagtattc cattggagca tcgaatgttg gtatcggaca
     2881 tgacggatta agttttataa gtttgaaatc ttctaccttg ggtaggagat ttttttatac
     2941 gatgggcatg tcgtgcatct gaggtgtaag tcctctgtag ggatccacta ccggtgaata
     3001 caatagcgct ccccaagcct gactatcgtg aggtagcgga ttaaaatggt ctggggatag
     3061 atcgttttaa gtctgacgca ggaaataaga attgtcagag aaagcaatag tgaaatcgta
     3121 ggctatgaat agaaacgtga tagcgaggag catatagtgg ataaggtagc gagaaatttt
     3181 aaagtataaa agatagtctt aatctatata tgtaaatcac aaaagtaatt gaattcggaa
     3241 taaggttggt gtgaaaagac atagtaaact gaatcttgaa tactagaatg atacttctac
     3301 aatattgtta taatttaatt tgaattccaa aatcacattg ttccgtccgt atttcatttt
     3361 actgtatgtc ttcctaaact ccaaagtttc tgcgtcaaat ttcctatttt cactgtagtc
     3421 tttgacactt cgaacgacaa tttgaatcat atattcaaaa aaaaatgaaa aatttgtgcc
     3481 aacaagccta aatattctga aaatttgttt gatttttaat ggataatgtg atataatggt
     3541 tgtcaatcta taattcacat aaacagagga gtagaaaatg aaaaaaaatc gtgtatttgc
     3601 tacagcaggt cttgttttat tagcagcagg tgtacttgca gcatgcagtt cttcaaaatc
     3661 atctgattca tcagccccta aagcttatgg ctatgtttat acagcagacc cagaaacctt
     3721 ggactacctg atttcaagta aaaatagtac aacagtagtg acttcaaatg ggattgatgg
     3781 tttattcact aacgataatt acggtaatct tgctcctgca gttgcagagg attgggaagt
     3841 ctctaaggat ggtttgacct acacttataa gattcgtaaa ggggttaaat ggtttacctc
     3901 tgatggagaa gaatatgcag aggtgacggc taaagatttc gtgaacggtt taaaacacgc
     3961 agcagataaa aaatcagaag ctatgtattt agctgaaaat tcggttaaag gcttggcaga
     4021 ttatctatca ggaacttcaa cagatttttc aacagttggt gtcaaggcgg ttgatgatta
     4081 tacgttacaa tacactttga accagcctga accgttctgg aactctaagt tgacctattc
     4141 tattttctgg cctctgaatg aagaattcga aacatcaaaa ggaagcgatt ttgctaaacc
     4201 aacagatccg acatccttgc tttataatgg tccattcttg ttgaaagggt tgactgcaaa
     4261 atcttctgta gagtttgtaa aaaatgagca atattgggat aaagaaaatg tccacctaga
     4321 tactatcaat ctagcttact atgatggatc agatcaggag tcgctagagc gtaacttcac
     4381 tagtggagct tatagttatg cccgtcttta ccctaccagc tccaactatt ctaaggttgc
     4441 agaagaatac aaggacaata tctattacac acaatcaggc tctgggattg ctggtctggg
     4501 tgtgaatatt gatcgccaaa gttacaacta tacttctaaa actacagatt cagagaaagt
     4561 agctactaag aaggcattgc ttaacaaaga tttccgtcaa gccttgaatt ttgctcttga
     4621 tcgctcagct tactcagctc aaatcaatgg taaagatgga gcagctttag cagttcgtaa
     4681 tttatttgta aaaccagact ttgtttcagc tggtgagaag acctttggtg atttagtcgc
     4741 tgctcaactt cctgcttatg gtgatgagtg gaaaggtgtg aatttagctg atgggcagga
     4801 tggtttattc aatgctgaca aggccaaggc agagtttgcg aaagctaaga aagctttaga
     4861 agcagacggc gttcagtttc ctattcatct ggacgttcca gtagaccaag catcaaaaaa
     4921 ctacatatct cgtattcagt cctttaaaca atctgtagaa acagttcttg gtgttgaaaa
     4981 tgtcgttgtt gatattcaac aaatgacaag tgatgaattc cttaatatta cttactatgc
     5041 tgccaatgct tcatctgagg attgggatgt atcgggagga gtttcatggg ggccagacta
     5101 tcaagaccca tctacttacc tggatatttt aaaaacaact agcagtgaaa ctacaaaaac
     5161 atatttagga tttgataatc caaatagccc ttcagtagtt caagttggtt tgaaagaata
     5221 cgataaatta gttgatgaag ctgccaaaga gacaagcgac ttgaatgtcc gttatgaaaa
     5281 atatgcagcg gctcaagcat ggttgacaga tagttcactc tttattcctg ctatggcttc
     5341 ttctggtgca gcaccagtgc tttcacgaat tgttccattt actggagctt ctgcgcaaac
     5401 aggctctaag gggtcagatg tttacttcaa atatttgaaa ttacaagata aagtggtgac
     5461 taaggaagag tatgaaaaag ctcgtgaaaa atggttgaaa gaaaaagctg aatcaaatga
     5521 gaaagctcaa aaagaattgg caagtcatgt gaagtaaata atttctaata gaactttctc
     5581 tccatgagga gaaggttctt ttgggatttt taaaggaaat aatatgaaaa aatatatttt
     5641 tatgcgtgtt ttgcggtcat tggtttcgat tttcttagta acgactttga cctacacgat
     5701 tatctatacc ttggttcctc gaaaattgat tttcaagcag gatcctaact ataataaaat
     5761 tgcaacaacg gctgataaac gtgataacta tgaaaatact gtgtttgagc gtatgggcta
     5821 cattgagtat tacgatacta aagagttgca agaaaaggca agtagcatgg attcttctgt
     5881 aacagtagaa gcaaatgcga ccaataaagc tatttatgaa aagtacatca atcaattagg
     5941 tcatggttgg actttgggag aatttactga aagtggtcaa ttctatgcta ctcgtgaaat
     6001 tccaattttt gaacgtgttt ttcacttcta tgctaacttg attgacattg accatacaaa
     6061 taaaatccaa gaccctgaaa atccagactt gaaacgctac cttcgttttg aaaatgatcc
     6121 agctatcgga tggtcattgg tcggttcagg aactaaacat aaatatctct tgtactttaa
     6181 cagtcagttc ccatttgttc atcaaaactt tgtgaacttg aatttaggtg actcttaccc
     6241 aacctatgct aatacaccag ttcttcaggt tattactcaa ggtcaaggac aaaccaaaac
     6301 tgcccaagtt cagttcccaa caggtaagaa aacgtcttct gtaaatattt actcaagaac
     6361 ctacaagtca cctagtcagg ctgactctcg tgaagtagct agctatggga aagatgatcc
     6421 ttatacagcg actgaaagta attaccaata tccatctatg attgtcagct ctgctattac
     6481 tggtttgatt ggtttggttc ttgcctatgc tcttgccgtg ccacttggtt cagccatggc
     6541 tcgtttcaag aacacttgga ttgatagcct ctcaacaggg gctttgacct tcttgcttgc
     6601 tcttccaacg attgccttgg tttacatcgt tcgattgatt ggatcatcta ttgcccttcc
     6661 agattcattc cctatcttgg gagctggaga ttggcgttct tacgttttac cagcagtcat
     6721 ccttggtttg ttgggtgctc ctggtacagc catttggatt cgtcgttaca tgattgactt
     6781 gcaatctcaa gactttgttc gtttcgctcg tgcaaaaggt ttgtctgaaa aagaaatttc
     6841 aaacaaacac atctttaaaa atgccatggt tccgctggtt tcaggaattc ctggtgccat
     6901 cattggggtt atcggtggtg caacccttac tgaaacagtc ttcgccttcc caggtatggg
     6961 taaaatgttg attgactctg taaaagcatc taataactct atggtcgttg gtcttgtctt
     7021 catctttaca tgtatttcta tcttctcacg tcttttggga gatatttgga tgactattat
     7081 tgacccacgt attaaattga ctgagaaagg aggcaaataa tgtctacaat cgataaagaa
     7141 aaatttcagt ttgtaaaacg tgacgatttt gcctctgaaa ctattgatgc gccagcatat
     7201 tcttactgga aatcagtgtt taaacaattt atgaagaaaa aatcaactgt agtcatgttg
     7261 ggaatcttgg tagccatcat tttgataagt ttcatctacc caatgttttc taagtttgat
     7321 ttcaatgatg tcagcaaggt aaacgacttt agtattcgtt atatcaagcc aaatgcggag
     7381 cattggttcg gtactgacag taacggtaaa tcgctctttg acggtgtctg gttcggagct
     7441 cgtaactcca tcctcatttc tgtgattgcg acagtgatta acttggttat cggtgttttt
     7501 gtcggtggta tttggggtat ttcaaaatca gttgaccgtg tcatgatgga agtttacaac
     7561 gtcatctcaa acatcccacc tcttttgatt gttattgtct tgacttactc aatcggagct
     7621 ggattctgga atctgatttt tgccatgagc gtaacaacat ggattggtat cgccttcatg
     7681 atccgtgtgc aaatcttgcg ctatcgtgac ttggaataca acttggcatc acgtactttg
     7741 ggaacaccaa ccttgaagat tgttgccaaa aatatcatgc ctcaattggt atctgttatt
     7801 gtgacaacca tgactcaaat gcttccaagc tttatctcat acgaagcctt cttgtctttc
     7861 ttcggtcttg gattaccgat tacagtgcca agtttgggtc gtttgatttc ggattattca
     7921 caaaacgtaa caaccaatgc ttacttgttc tggattccat tgacaaccct tgtcttggta
     7981 tccttgtccc ttttcgtagt tggtcaaaac ttagcggatg ctagtgatcc acgtacacat
     8041 agataggagt agaaatgaca aaagaaaaaa atgtaatttt gactgctcgc gatattgtcg
     8101 tggaatttga cgttcgtgac aaagtattga cagccattcg cggcgtttcc cttgaactcg
     8161 ttgaaggaga agtattagcc ttggtaggtg agtcaggatc aggtaaatct gttttgacaa
     8221 agaccttcac aggtatgctc gaagaaaatg gtcgcattgc ccaaggtagt attgactacc
     8281 gtggtcagga tttgacagct ttatcttctc acaaggattg ggaacaaatt cgtggtgcta
     8341 agattgcgac tatcttccag gacccaatga ctagtttgga ccccattaaa acaattggta
     8401 gtcagattac agaagttatt gtaaaacacc aaggaaaaac agctaaagaa gcgaaagaat
     8461 tggccattga ctacatgaat aaggttggca ttccagacgc agatagacgt tttaatgaat
     8521 acccattcca atattctgga ggaatgcgtc aacgtatcgt tattgctatt gcccttgcct
     8581 gccgacctga tgtcttgatc tgtgatgagc caacaactgc cttggatgta actattcaag
     8641 ctcagattat tgatttgcta aaatctttac aaaacgagta tcatttcaca acaatcttta
     8701 ttacccacga ccttggtgtg gtggcaagta ttgcggataa ggtagcggtt atgtatgcag
     8761 gagaaatcgt tgagtatgga acggttgagg aagtcttcta tgaccctcgc catccatata
     8821 catggagtct cttgtctagc ttgcctcagc ttgctgatga taaaggggat ctttactcaa
     8881 tcccaggaac acctccgtca ctttatactg acctgaaagg ggatgctttt gccttgcgtt
     8941 ctgactacgc aatgcagatt gacttcgaac aaaaagctcc tcaattctca gtatcagaga
     9001 cacattgggc taaaacttgg cttcttcatg aggatgctcc aaaagtagaa aaaccagctg
     9061 tgattgcaaa tctccatgat aagattcgtg aaaaaatggg atttgcccat ctggctgact
     9121 aggaggaagg aaatgtctga aaaattagta gaaatcaaag atttagaaat ttccttcggt
     9181 gaaggaagta agaagtttgt cgcggttaaa aatgctaact tctttatcaa caagggagaa
     9241 actttctcgc ttgtaggtga gtccggtagt gggaaaacaa ctattggtcg tgctatcatc
     9301 ggtctaaatg atacaagtaa tggagatatc atttttgatg gtcaaaagat taatggtaag
     9361 aaatcgcgtg aacaagctgc ggaattgatt cgtcgaatcc agatgatttt ccaagaccct
     9421 gctgcaagtt tgaatgaacg tgcgactgtt gattatatta tttctgaagg tctttacaat
     9481 caccgtttat ttaaggatga agaagaacgt aaagagaaag ttcaaaatat tatccgtgaa
     9541 gtaggtcttc ttgctgagca cttgactcgt taccctcatg aattctcagg cggtcaacgt
     9601 caacgtatcg gtattgcccg tgccttggtc atgcaaccag actttgttat tgcagatgag
     9661 ccaatttcag ccttggacgt ttctgtacgt gcccaagtct tgaacttgct caaaaaattc
     9721 caaaaagagc tcggcctgac ctatctcttc atcgcccatg acttgtcggt tgttcgcttt
     9781 atttcagatc gtatcgcagt tatttacaag ggtgttattg tagaggttgc agaaacagaa
     9841 gaattgttta acaatccaat tcacccatat actcaagcct tgctttcagc ggtaccaatc
     9901 ccagatccaa tcttggaacg taagaaggtc ttgaaggttt acgacccaag tcaacacgac
     9961 tatgagactg ataagccatc tatggtagaa atccgtccag gtcactatgt ttgggcgaac
    10021 caagccgaat tggcacgtta tcaaaaagga ctaaactaat aatggtttta taatttccat
    10081 gtcaattttt atgggaatta taaaactttt ttgttggact gatttgaaaa actttgaaag
    10141 cttcctgaaa ga
//
LOCUS       DAVBCW010000070        10030 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVBCW010000070 DAVBCW010000000
VERSION     DAVBCW010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10030
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.070"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(28..1731)
                     /gene="ilvD"
                     /locus_tag="VQ514_001465"
     CDS             complement(28..1731)
                     /gene="ilvD"
                     /locus_tag="VQ514_001465"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET2500213.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(1964..2896)
                     /locus_tag="VQ514_001466"
     CDS             complement(1964..2896)
                     /locus_tag="VQ514_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HET2500214.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSDSDIVVLEADLSSSMATNNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     GVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSALCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(2893..3750)
                     /locus_tag="VQ514_001467"
     CDS             complement(2893..3750)
                     /locus_tag="VQ514_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET2500215.1"
                     /translation="MILSKNREDGLRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEEKQMLTSVVER
                     LSQELEETE"
     gene            complement(3754..5100)
                     /locus_tag="VQ514_001468"
     CDS             complement(3754..5100)
                     /locus_tag="VQ514_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HET2500216.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFVGGLVSMVIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSATEE"
     gene            complement(5113..5397)
                     /locus_tag="VQ514_001469"
     CDS             complement(5113..5397)
                     /locus_tag="VQ514_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2500217.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(5401..7431)
                     /locus_tag="VQ514_001470"
     CDS             complement(5401..7431)
                     /locus_tag="VQ514_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HET2500218.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NSHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLIDQVVKDYYEKLQLVPIVNQLKVYLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKWYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
     gene            7623..8630
                     /locus_tag="VQ514_001471"
     CDS             7623..8630
                     /locus_tag="VQ514_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET2500219.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKQISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            8633..8833
                     /locus_tag="VQ514_001472"
     CDS             8633..8833
                     /locus_tag="VQ514_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HET2500220.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            8985..9167
                     /gene="rpmF"
                     /locus_tag="VQ514_001473"
     CDS             8985..9167
                     /gene="rpmF"
                     /locus_tag="VQ514_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HET2500221.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKITKAASAE"
     gene            9183..9332
                     /gene="rpmG"
                     /locus_tag="VQ514_001474"
     CDS             9183..9332
                     /gene="rpmG"
                     /locus_tag="VQ514_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET2500222.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(9824..>10030)
                     /locus_tag="VQ514_001475"
     CDS             complement(9824..>10030)
                     /locus_tag="VQ514_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HET2500223.1"
                     /translation="YLNESGSMATGWVKDKGLWYYLNESGSMATGWVTVSGKWYYTYN
                     SGDLLVNTTTPDGYRVNANGEWVG"
BASE COUNT         3248 a         2133 c         1620 g         3029 t
ORIGIN      
        1 gatttgaaga gattttcgag tatgagttta ttttttacct gacttgtcca tattccagaa
       61 gtctgtcacg gctccgcgtg aagcagatga tacgatgtgg gcatatttac cgaggacacc
      121 acggctgtaa agtggtggca aggttgtttc tgccttgcgt ttttcaagtt cttcttcgga
      181 tacggccatg gaaatttctt tggtatcttg gtcaaccgta acgatatcgc cggtacggag
      241 ataggcaatt ggtccaccat cctgagcttc aggagcgata tgtccaacaa ccagaccata
      301 agtaccacca gagaaacggc cgtccgtcaa gagggccacc ttatctccct gacctttacc
      361 aacaatcatt gaagaaagtg acagcatctc aggcatacca ggaccacctt taggtccaac
      421 aaaacgaaca acgactacat cgccatcaac gatttcatct gtcagaacgg cctgaatcgc
      481 atcttcttct gagtcaaaga ccttagctgg cccaacgtga cgacgcactt taacacctga
      541 taccttggca actgcaccgt caggagcaag gttcccgttc aagatgataa gcggaccatc
      601 cgcacgtttt ggattttcaa gtggcatgat aactttttgg cctggagtca agtctgcaaa
      661 gtcagccaag ttttcagcta cagtcttacc agtacatgtg atgcgatctc cgtgaaggaa
      721 accatttgcc aacaagtact tcataaccgc agggacacca ccgacttcgt agaggtcttg
      781 gaagacatac tgaccagatg gtttcaagtc ggccaagtga ggcacacgtt cttgaatcgt
      841 attgaagtcc tcaagtgaca agtcaacatt tgcggcatgg gcaatggcga gcaagtgaag
      901 agtggcgttt gtagaaccac cgagagccat cgttacagtg atagcatctt caaaggcttc
      961 acgagtcaag atatctgatg gtttgagacc aagttccaac atcttaacaa cagcacggcc
     1021 tgctgcttcg atatcttctt tcttatcagc tgattcagct gggtgagagg atgaccctgg
     1081 caaactcatc cctagaactt cgatagcagt tgccatggta ttagcagtat acataccacc
     1141 acagccacca gggccagggc aggcattaca ttcaagacgt ttcacgtcct cagctgtcat
     1201 gtcaccgtgg ttccattttc cgataccttc aaagacagaa accaagtcga tatctttacc
     1261 atcaagattt cccggtgcaa tagttccacc ataggcgaaa atagctggga tatccatatt
     1321 agcaatagca atcatagatc caggcatgtt cttgtcacag ccaccgatag cgacgaaggc
     1381 atccacgttg tgaccactca tagccgcctc gatggagtcc gcgatgatgt cacgagatgt
     1441 tagagagaaa cgcataccag gcgttcccat agcgatcccg tccgctacgg taatggttcc
     1501 aaactgtaca ggccaagcgc ctgcagattt gacaccttct ttagccagtt tcccgaaatc
     1561 atgcaagtga atgttacatg gtgtattttc cgcccaagtc gaaatcactc ccacaatcga
     1621 tgtttcaaag tccttatctg tcataccagt cgcacgaagc atagcacggt taggtgattt
     1681 aaccatgctg tcatagatgc tactgcggtg acgtttatct aattcagtca tcttatccct
     1741 cccatttcag tttttactat tatagcacaa ttttcgcatg aagaacagaa taaaattctt
     1801 gaattttcag aaaattctat acacatgtga aatatttaaa attaaaaaca acaaagcgga
     1861 ttagtgcact ttctgatgac cagaatatgc tttttaatcc gctttcttta aataacgtac
     1921 tgtaattttt acagaaattc tttcaaataa gtgtatttaa catctatctt gcattataaa
     1981 tttctagaac cttctctttt atattcgatt cactcaaacc atactcatta agaagataat
     2041 ccattttccc tacttgaccg aatctttctt gaacacccat ccgatgaatt tttgttattc
     2101 catcatcaga gaataattca cataaagcac tgccaattcc acctatctga ttgtggtttt
     2161 ctacagtaaa tatagttttt ccacttaaca ttgtttttat ctgttctggt atcggtttga
     2221 ttctaaataa atctatcaca cctactgaat aacctaattt agacagttca tctgcaactc
     2281 gaatacttgg agcaaccatt atgccagaag caacgattac aagatcttca ccatgcctta
     2341 actcaatgta gcctttagaa aaatcttctc caccttgata cacaggaact ggagcttttc
     2401 taattgttcg aatatatttt agtcctttta agtctaatgt ctggttcaat atttcacgaa
     2461 attggatatc atcagttgct tcgaaaatga ttgatttagg aattaaacgt aacaatccaa
     2521 tttcttcaaa tggcatatgt gttccaccat tcatctctgc cgttactcct gcatctgatc
     2581 caatcacagt ggcatccaat tgtgcgtatc caagagaaat aaataattga tcaaatactc
     2641 ttcgtgaagc aaaaggacca aatgtatgaa gataaggtct aaacccctga atagacaagc
     2701 ctgctgcaag gccgaccatt tctgcttcca taatcccaac attcacataa cggtctccaa
     2761 agtccttttc aagattatta gtagccatcg aacttgacaa atcggcttct aagactacta
     2821 tatcagaatc actttgatta gcctctaaaa ggaagtctct atatacatgc cgtaattctt
     2881 tcgtacttct catcattctg tttcctccaa ttcctgactt aatctttcta caactgaagt
     2941 taacatttgt ttctcctcta cagtagggcg aagatgatga ttggatttca tttcttccag
     3001 ctcttgaacc ccttgacctt taatagtatc taatacaatg cacttaggtg atgaattatt
     3061 tgactgtttt aattggacaa tcccttcata aatttctcta atatctgaac ccttgaccct
     3121 aatggattca aatccaaatg ctgaaaattt ttctacgaaa tcacctggat tacaaatatc
     3181 ctttgtaaaa ccatctaatt gttttttgtt atcatcaaca aatacaatta agttggataa
     3241 ctgttgatga gaagcaaact gtatagcctc ccaacattgt ccctcattta actcaccatc
     3301 tccaacaata gcgtaagtat aaaagggact ctttcttatt ctctgaccat atgcaagtcc
     3361 agttgcaaca ctaattcctt gtcctaaaga gcccgttgtc atatctatgc ctggcgttag
     3421 atttctatca ggatgagacg gtaatttggt tccatttgta tttaaagaat ataagaattc
     3481 tttgtcaaag aaaccattca aatagagtgt actgtataga gctggtcctc cgtgaccttt
     3541 tgataatatg aaataatctc tatctcgtgc tgcaaatatt tctggagtca ttggcattat
     3601 ttcaccataa agcaccgcta aaacttctac gatagacaga cttcctccgt aatgtccgaa
     3661 tccaagatga ttcaatgttc taagagtatt taatcggatg ttagtcgcaa attttcttaa
     3721 cccatcttct ctatttttac ttaaaatcat cccttattcc tccgttgcag atggcttttt
     3781 aataaaggat actccaaaca taactgctag aataagaaca agaccaatca caatgcctgc
     3841 ttgtgagcca aattgattta acattcctaa aataattcct gatagaccaa aatctgcatc
     3901 tgagaaagtt gatccttgga aaccaagtcc tcccaaaact ggcattaaaa agactggaag
     3961 aaaactgatt aaaatacctt gtaaaaatgc tccaatagtg gctccacgaa caccaccaga
     4021 tgcattccca atgacacctg cagtcgctcc acagaagaaa tgaggcacaa cacctggtaa
     4081 gataacaacc gttcctgaag caatcataat taccatactt actaaaccac caacaaaact
     4141 agagataaat ccaattagaa ctgcattggg tgcataagta taaacaatcg gacaatccaa
     4201 agcaggtttt gaattaggta caagacgctc tgaaatacct ttaaaggctg gaacaatttc
     4261 gcccaaaata aggcgaacac ctgctaaaat aacaaatacc cctgctgcaa attgacctgc
     4321 taattgtaaa gcataaacta gaccacttgt accactactg atttcttttt ctatatattc
     4381 tgaccctgca aagatagcta caataatgta aataactgcc atggataaag taatactaac
     4441 agtactatca cgtaaaaaag ctaaactctt tggaaattta atgtcctctg ttgattttga
     4501 tttgtcaccg ataaggctac cagtaaaacc actcaaccaa tatcccaaag aactgaaatg
     4561 acctaaagct accttgtcat ttccagttaa ttgaaccata tatttttgca caaatgctgg
     4621 ggaaatactc ataataatac cgagtgctaa tcctcctagt aagatgagag gcaagctagt
     4681 aaagccagca actgataaaa tgaccgcaat catacatgcc atatatagag tgtggtgccc
     4741 tgttaaaaaa atatatttaa atcgagtaaa acgagcgatt aagatattga acaccatgcc
     4801 tgcaaacata atcattgcag tagctgagcc atatgttgtt aaagctacag ctacaattgc
     4861 ttcattattc ggcacaacgc cagataaatg aaaagcatgc tcaaacatgg taccaaatgg
     4921 atttaaagaa ttttgtacaa ttcctgcacc accagataca actaagaaac caacaaaggt
     4981 cttaattcca cctttaataa tatcaggtaa tttcttcttc tgaagaacta atcctaagat
     5041 tgcaattaaa gctactaaaa tagctggtgt actaacaata tccaatatga acttcatcat
     5101 gacgctagcc tcctatataa gtcctttttc ttcacaaagt ttagtaatta attctcgtag
     5161 ttcatccata tcaataatac tatttaagat acgaacatct ccaagatgac tagctgaatc
     5221 agctagatca cgaccaacaa tccaaatatc agctgcattt ggatctgctc cacctaaatc
     5281 ataatgttca acttctacat ccgaaacatt caaatcactc aatacagatt caatattcat
     5341 ctgtaccata aaacttgaac ctaatcctga accacaagct gtaccaattt ttaacattat
     5401 ctaatcctcc tgtttaatta tcattttaat gtcatcatag ttttttgatg atattaaagt
     5461 ttgaacatga tttttatctc ttaaaattgt tgttaaatgt gacaaagcct ttaaatgact
     5521 ctcattatca atggctgcaa tacaaatcaa caatcttacc tcttgttctg gattatccaa
     5581 taaataaatc ggttcttcca aaactaacat tgacattcct atttcattca caccttcatc
     5641 tggccgagcg tgaggaattg ctactccctt ccctaaatta ataaaaggtc caaactcttc
     5701 tactttttga atcattgcct cagggtagtt ctcagttatc ttatcttgat ccaaaagcgg
     5761 tttagctgct aaacgaatcg cctccttcca tcctaatttt tgcgaactaa cctgataggt
     5821 ttctttggta ataagttgtt ctagcactgg tacaatttcc tttctatcat ttttttggta
     5881 aagataattc tttaacgcca atcttaattc caattcttgt gtaataattc catatctttt
     5941 gacaatattc aggatttgtt caatctcaaa atctccatac tctaaattcg gaaaatcttt
     6001 taacactagt tctactagtt gtattgcttg ctcttcagtc atcataaccg aaactagata
     6061 atttggcttt tctgtctcca cctttatggt agaaaaaacc atatcatagt cactactagc
     6121 tttcacctgt aaatcatcaa tctttgaggt tcctataaac tcaatttgag gaaataatgc
     6181 taatagattc tcttttaaca tcaatgaaga actaacacca ttaggacaaa tgattgctgc
     6241 tttataccat ttttgaggca aagtatctgc tttctttaaa taacctccga aatggataac
     6301 aaaatatgct gtttcactat caggtatggg attgtcaata gcgtccatca agggcatcaa
     6361 agaatctttg actaattcaa ataaatcagg ataatgttct ttaacatgca atacatattc
     6421 atttgaacta ggtaggccga actttaatct atagtaagcc ggtataaggt ggcggcgaag
     6481 attttctctc aatccttccc tttgtttaaa atgtaacaaa gaaatatctt ccattctact
     6541 tataatagcc tctgttaatt gattaaagta aaccggagca acatctactt caccttcaaa
     6601 gcaacttgat aataaaacgg tgatatagcg ataatcatcc tcagaaaaca ccgtatctat
     6661 aattcccaaa tcaaccactg tatccaataa aatagtggtt atatcttgaa taacaggaga
     6721 tactaatgtc tctgaaagac atactctttc aacatccctt tgatacctac acagaatgaa
     6781 tactaaaccg aaaaggtaaa cttttaattg attaacaata ggtactagct gtagcttctc
     6841 ataataatct ttaactacct gatcaatcaa atcataagtt aatgaatacc cccaactgga
     6901 taaaacataa tccaaacccc aaatccctat ggaggattcc agcaactcac taaccatttg
     6961 aaaagctaag cggtgcttat tccactctga accgtgtaaa gtataacctt ttgctctact
     7021 gtaccctagc tccaaatcat tatctaacat aatctttctt aatgattgaa tatcagataa
     7081 ggttgtattc ttacttactt tcaaaaagtc ttggtaatga ctattcgata taaaatctaa
     7141 tcggcaaaaa gtgtaaagat agattaaagc taagcgagtc gactttggta aaaccaattc
     7201 atccgactta ataatatctg tcaaagactg cttcgtacga tttgataaac tatagcgacc
     7261 ttgcttttta tccagcacta tccctttatt agctagataa ggcactaaat aatctattcc
     7321 ttctttgact tcctttatag gtaagctcac cttaacagat aattcatata atgatagctc
     7381 acaatgatcc atcaaagtca tcaaaataac tagtgctcta taatcaaaca tttctcttcc
     7441 tttctaacta caattatagt gtaaaaatta taagaataaa agagtatttg agcacaatat
     7501 aatctcacct tttaaaagaa attattgggc tgaaagtttt tagtgattat ttttcaactt
     7561 tctattgact ttataaatta aagtgatatc atttagatat caaataacag gaggtcattc
     7621 ttatgactga aaaactaatc aattcaaaac caaatggtgt attcgcattg attctcattg
     7681 agttgacaat cgtacttggt atctttatat ttataatggg ggttggttcg gaaaacattt
     7741 ttggaattat tatcggacct ttactaatcg taattgcagg tctagctcat gctggtttaa
     7801 aagttgtcaa acctcaagaa gctctggttc tgacactctt tggtaactat acaggtacca
     7861 tcaaagaacc tggcttttac tttgtcaatc ccttcagcgt agcagtcaac cctgcaaacc
     7921 acactcgact tggacaaagt ggtgatgtta gcacaaaatc tcctttttta ggagctaaat
     7981 catcaaatga caatgatgta aatcttgaaa ttggcaagaa acagatttcc ctcaaagtca
     8041 tgaccttgag caattctcgt caaaaaatca atgattgctt aggaaaccct gtagaaatcg
     8101 gtatcgcggt aacttggaga gttgtcgata ccgctaaggc agtcttcaac gttgataact
     8161 acaaagaata tctttcattg caatgtgata gcgccctccg taatattgtc cgcatctatc
     8221 cttacgatgt gtctcctaat gtggatacta cgggtgatgg gcaagcagat gaaggtagtc
     8281 tccgtggctc tagcgaaatt gttgctaacc gtattcgtga agaaatccaa agtcgtgttg
     8341 aggatgctgg cttggaaatc cttgaagcac gtatcactta cctagcttat gctccagaaa
     8401 ttgctgccgt tatgcttcaa cgccaacaag catctgccat tattgatgca cggaagatga
     8461 ttgtagatgg tgctgtagga atggttgaaa tggcactaga acggctcaat gaaggggaat
     8521 tggtagaact tgacgaagaa cgaaaagctg ccatggtttc aaatctcctt gtcgttcttt
     8581 gtggcaatca tgatgcacaa ccaattgtca acacaggaag cctttactaa agatggctga
     8641 agctaaaaaa aagcagatcc cccttcgact ctcaacaaag ttatacgctg cactcgcatc
     8701 atgggcagaa gacgactttc gttcagtcaa tgggcaaatc gaatatctcc tcacagagtg
     8761 tgtcaagcaa cggaagaaag acggaaaata cgtatcagaa accattgacg aaccatttga
     8821 gattgatatt taacaccttc tcctgtctgc cgtgtcggat aggagaattt tttcatcttt
     8881 ttttgtcaag taactttact ttacaaaaaa aatgtgttat cctagtatgg ttgatgaaaa
     8941 tcagtagatt gaatcgaata taaataccta aaggagaaat caaaatggca gtacctgcac
     9001 gtcgcacttc aaaagcgaag aaaaacaaac gtcgtacaca ctacaaagta acagctccat
     9061 ctgtaaactt tgacgaaact actggagatt actcacgttc tcaccgtgta tcacttaaag
     9121 gatactacaa aggacgtaaa atcactaaag ctgcatcagc tgaataatag aagggagata
     9181 ccatgcgcgt aaatattaca cttgaacaca aagaatctgg tgaacgcttg taccttactt
     9241 ctaaaaacaa acgtaacact ccagaccgcc ttcaattgaa gaaatactca ccaaaacttc
     9301 gcaaacacgt tgtgtttaca gaggttaaat aagcattcaa tacgaatcaa tacagaagaa
     9361 aaacgcttga tcagcgtttt ttctttttgt taatttcact acattgtaag ggaaatcatt
     9421 cgaatgacta catttttcac tacctttttt gaaataagaa tacggattcg gacaattaaa
     9481 agcaagagga actcaaatgt aagttggggt tccttttttg tatatttttc aaataacgcc
     9541 tctacactat ttgtagcaaa ttcaccaact acagttgtat cttagttaaa ataagttaga
     9601 atatgtaagt gagtaccaga tataccaaga catcgtcacc atctaaggta tattcaaaat
     9661 acaaaagttg accaactaga tttctgaata tccttatata tccattctta aaattggttt
     9721 aaatagcgta gtcttttaaa ctagttttga gaatccaaaa aatcttccta catatgtaag
     9781 aagatttttt agttcagaat gattagattt agctaatgga tacctatcct acccactcac
     9841 cgttagcatt gactcgatag ccatcgggtg tagtcgtgtt tactaataaa tctcctgaat
     9901 tataggtata gtaccattta ccagaaactg taacccaacc agtagccatt gaacctgatt
     9961 catttaagta ataccataag cctttgtctt taacccaacc agtagccatt gaacctgatt
    10021 catttaagta
//
LOCUS       DAVBCW010000071         9760 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVBCW010000071 DAVBCW010000000
VERSION     DAVBCW010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9760
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.071"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            271..366
                     /locus_tag="VQ514_001476"
                     /pseudo
     CDS             271..366
                     /locus_tag="VQ514_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(619..1239)
                     /locus_tag="VQ514_001477"
     CDS             complement(619..1239)
                     /locus_tag="VQ514_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET2500224.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLYTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(1239..1721)
                     /locus_tag="VQ514_001478"
     CDS             complement(1239..1721)
                     /locus_tag="VQ514_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HET2500225.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDRMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(1721..2563)
                     /locus_tag="VQ514_001479"
     CDS             complement(1721..2563)
                     /locus_tag="VQ514_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HET2500226.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDLKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDLLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(2658..3458)
                     /locus_tag="VQ514_001480"
     CDS             complement(2658..3458)
                     /locus_tag="VQ514_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HET2500227.1"
                     /translation="MRKQIHPLILFSFFGIMMFILIINRPLNDSQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            3602..4981
                     /gene="glmU"
                     /locus_tag="VQ514_001481"
     CDS             3602..4981
                     /gene="glmU"
                     /locus_tag="VQ514_001481"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HET2500228.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            4991..5536
                     /locus_tag="VQ514_001482"
     CDS             4991..5536
                     /locus_tag="VQ514_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2500229.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTARRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            5548..5862
                     /locus_tag="VQ514_001483"
     CDS             5548..5862
                     /locus_tag="VQ514_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HET2500230.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            5879..6571
                     /locus_tag="VQ514_001484"
     CDS             5879..6571
                     /locus_tag="VQ514_001484"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HET2500231.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNTQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            6648..7139
                     /gene="rocS"
                     /locus_tag="VQ514_001485"
     CDS             6648..7139
                     /gene="rocS"
                     /locus_tag="VQ514_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HET2500232.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            7237..7827
                     /locus_tag="VQ514_001486"
     CDS             7237..7827
                     /locus_tag="VQ514_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HET2500233.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGVLDLSQLFD"
     gene            7836..9599
                     /locus_tag="VQ514_001487"
     CDS             7836..9599
                     /locus_tag="VQ514_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HET2500234.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLNLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT         3005 a         1698 c         2084 g         2973 t
ORIGIN      
        1 caccccaaaa gttagatttt ttctgtctaa cttttggggt gtagttcatg tacacctgat
       61 atgatgcgtt ttataatttt aaagactttt tgaccagcct cattttttta acttgatact
      121 cagtgaaaag caaagattaa actaggaagc tagctgtagg ctgctcaaag aacagctttg
      181 aggttgcaga taaaacttgt gaggtcacca acatatataa tgtgaagctg acgtggcttg
      241 aatagatttt agaagggtat gagtctggaa gttttaatgg ataatgcaag attccataga
      301 atgggtaagc tagagttctt atgtgaagag tttgggcata aacttttacc ttttcctccc
      361 tactcatctt agtatagaaa agtgaatcta aaatagtaca taactgcttc taaaacattc
      421 ttataaattg atttaaattc tcaaatcata ttattcagtt cttatttcat tttgctctac
      481 aatcctgttg agaagacacg tgttcatatc aaaaaggtat tggcaagttg caataccttt
      541 ttacaaggtt cttttgtctt atttttgttt caactgacta tatctcctat ggttctagtt
      601 cagaaggcta ggctataatt atgattgata agaagtatca ttccaagtat tgagagtgaa
      661 tgtttcaaaa tcatgggttt ctataatggt caggctggca tttgctagac cgccatcttt
      721 acgaagaagt ggttctttat agcctaggag agtacgaaga ctggcagtaa gattggcgcc
      781 gtgtccgaca attagaatac gctcagctgg actatctttt aatgatttga taaattggat
      841 ggtccgttga gttgtactat agagggattc ggctccgaac attcgagtgt caaattgagc
      901 aagatttgaa cgaaaagcct ggatttgttg cgggtaaata gcttccaagg ttgcaatttt
      961 caaaccttct aacttcccaa gttgccattc acggagatta ggaacgattt ctaaagaaca
     1021 gggggtatag agttgacttt ggataatctc agcagatttg accgctcgag gtaaatcact
     1081 tgaataaatc tgatcaaaag gaatttcctt gagatactgg ccaagtcgtt ttagggtttc
     1141 aatggattca ggaagaagag gagaatcacc gctagcacct tgaaaacgac cttcttggtt
     1201 ccagagggta cgaccgtggc ggacaaagta gagtttcatt acttgatgtc ctccaaaata
     1261 tctacaaagt ctgcctttac aaagctagcc aagtcttgtg gcgcgacgat aatgctgtgt
     1321 ccgacttcgc ctgcagagac aatcattcga tccaaatcta gagcaatttt atcgataaaa
     1381 atgggataat tgtgtttctg acgaattccg acaggattat tggctccatg aatgtaacca
     1441 gttgtttttt ctaagtcctt ttgtggaatc atgctcactt ttttattgcc agaaatttta
     1501 gctagtttct tttcagacaa gtgctgagtg atagggacaa ttccgataat cggtccggtc
     1561 ttgtctccca aaagcgccaa ggttttgaaa atctgatctc gttcataacc ttgaggaagc
     1621 tctccttcta gggcattgat ttgaatcccc tgatgaggga tagctgcttt agataggatt
     1681 tgttccacca atgttttttt gattttaact ttttttgcca ttatttatat ttatcctcca
     1741 attgactcat ccaaatacca agccagattc ccagcgcaaa gaagaaggcg atgatgacat
     1801 aaccgacaag tgaaagtcct gtgtattgga tactttcagc gtttcctgca tttggaatta
     1861 agatcaaaag ggtacttgat aggacgatac cgatgatgaa atgatagacg cgtgagtggt
     1921 agttgtttaa ggcataatcc atcaattttg aaaaaacgat gagagttgca cctgcaccaa
     1981 ttccaatcgg aaagaaggtt cccaagagat caaaagtttt aaaaccagtc aacataggag
     2041 catagagtcc caaaatcaaa agtaagttcg atgggctgag gccaggaacc aagacgccaa
     2101 gggccaatag tgcgcctgct aggacgaagt taagaaagct ggcgcttaag gttccaacga
     2161 caaaatttaa ggcatagagt cctaatccag aaatgataaa ggttgtccat aaccaagcta
     2221 aatcaatctt gtctcggtca gattctcgag ttgattcttt gaggaggcta ggaactgtac
     2281 cgataatagc tcccgcaaag ctccataata caaagacctg ataattttca agcaggtatt
     2341 caatcgggta ggaaaataag ccgattccca gaagcatacc gatggcaact ggaataaagt
     2401 acaaaacatt ttctttaagg tctttaaagg gatgggccag aaagccaatc attcgttcat
     2461 agattcctaa gattgctgct agaacccctc cggaaattcc cggtaggata aatccaagag
     2521 caattacaat ccctttaata acgcgtgcta accatgagag catatatttc ctccaactat
     2581 ttctatttca aaaattcctt actatattgt atcacaatca gacacaaaaa gaaaaagcaa
     2641 atgataaaca aatgctttta aggttttaaa aaaagttttc gaaaggtttc ttctttattt
     2701 tttaagggag agataacgtt gatatctaaa tcgtggtcaa agccggcaat ttttccttta
     2761 gatgtgtatt ggtgaatatc ataatctaaa tcagttttag gactgctctc caaaaatcct
     2821 gagtctgagc cgtaggacgg aatccaaaca gaggtaaact tgcctgtatc aatactgtgt
     2881 tcttccatga agtagacacc aacgtagatg ccgatgtttt tagcacctag tgatgctagt
     2941 tttgctcgaa agttttcgac accttcgttc atattagaca tggttttgtc ttccacgtca
     3001 agccaatagt aactagggct gtaaggagag gcagcattgt agaaaacttc ggcagccttt
     3061 tccatttctt ggacactttt tccagctaca taagcgtaga cagcaactgg gacattccgc
     3121 ttttgaagtt cagtgatatg actcttatag gccttgtcta ttccattgat aaatgaagca
     3181 tcattttctt ttgttgtttg agcaccactg tgaacacgaa caatagcacc tgaaatattt
     3241 tgtgagaggg catcgtagtt gatttcctca ggacgctgcc agccagagag gtcaataatc
     3301 ggtttgtcta agtgtttcaa agcctgtgct tcaatctgtg ctatattgga ttttgtttta
     3361 aacgattggc tgtcattaag tgggcgattg atgattaaaa tgaacatcat aatcccaaaa
     3421 aaactaaata aaataagtgg atgaatttgt tttctcatat cttataagtc taccctaaaa
     3481 atcaaaaaaa atcaaaaaaa tgggttaagg aagagacttt agagcatttt ttcattcaag
     3541 agtgcggaat gatttgaaat atggtataat aaaagggaat ttctacagaa aagagaagat
     3601 tatgtcaaat tttgccatta ttttagcagc gggtaaaggg actcgcatga aatctgattt
     3661 gccaaaagtt ttgcacaagg ttgcgggtat ttctatgttg gaacatgttt tccgtagtgt
     3721 gggagctatc caacctgaaa agacagtaac agttgtagga cacaaggcag aattggttga
     3781 ggaggtcttg gctggacaga cagaatttgt gactcaatct gaacagttgg gaactggtca
     3841 tgcagttatg atgacagaac ctatcttaga aggtttgtca ggacacacct tggtcattgc
     3901 aggagatact cctttaatca ctggtgaaag cttgaaaaac ttgattgatt tccatatcaa
     3961 tcataaaaat gtggccacta tcttgactgc tgaaacggat aatccttttg gctatggacg
     4021 aattgttcgt aatgacaatg ctgaggttct tcgtattgtt gagcagaagg atgctacaga
     4081 ttttgaaaag caaatcaagg aaatcaacac tggaacatac gtctttgaca acgagcgttt
     4141 gtttgaggct ttgaaaaata tcaataccaa taacgctcaa ggcgaatact atattacaga
     4201 cgtcattggt attttccgtg aaactggtga aaaagttggc gcttatactt tgaaagattt
     4261 tgatgaaagt cttggggtaa atgaccgtgt ggcgcttgcg acagctgagt cagttatgcg
     4321 tcgtcgcatc aatcataaac acatggtcaa cggtgttagc tttgtcaatc cagaagcaac
     4381 ttatatcgat attgatgttg agattgctcc ggaagttcaa atcgaagcca atgttacctt
     4441 gaaagggcaa acgaaaattg gtgctgagac tgttttgaca aacggtactt atgtagtgga
     4501 cagcactatc ggagcaggag cggtcattac caattctatg attgaggaaa gtagtgttgc
     4561 agacggtgtg acagtcggtc cttatgctca cattcgtcca aattcaagtc tgggtgccca
     4621 agttcatatt ggtaactttg ttgaggtgaa aggatcttca atcggtgaga ataccaaggc
     4681 tggtcatttg acttatatcg gaaactgtga agtgggaagc aacgttaatt tcggtgctgg
     4741 aactattaca gtcaactatg acggcaaaaa caaatacaag acagtcattg gagacaatgt
     4801 ctttgttggt tcaaattcaa ccattattgc accagtagaa cttggtgaca attccctcgt
     4861 tggtgctggt tcaactatta ctaaagacgt gccagcagat gctattgcta ttggtcgcgg
     4921 tcgtcagatc aataaagacg aatatgcaac acgtcttcct catcatccta agaaccagta
     4981 ggagcctatc atggagtttg aagaaaaaac gcttagccga aaagaaatct atcaaggacc
     5041 aatatttaaa ctggtccaag atcaggttga attaccagaa ggcaagggaa ctgcccgacg
     5101 ggatttgatt ttccacaatg gggctgtctg tgttttagca gtaacggatg aacaaaaact
     5161 tatcttggtc aagcagtacc gcaaagctat cgaggctgtc tcttacgaaa ttccagccgg
     5221 aaaattggaa gtaggagaaa acacagcccc tgtggcagct gcccttcgtg aattagagga
     5281 agaaacagcc tatacaggga aattagaact cttgtacgat ttttattcag ctattggctt
     5341 ttgtaatgag aagttaaaac tatatttagc aagcgatttg acaaaagtgg aaaatccgcg
     5401 tccgcaggat gaggatgaaa ccttggaagt ccttgaagtg agcttagaag aagcgaaaga
     5461 attaatccaa tcaggtcata tctgtgatgc caagacaatt atggctgttc agtattggga
     5521 gttgcagaaa aaatagagga ggtcagtatg ggtaaatctt tattaacgga tgaaatgatt
     5581 gaaagagcta atagaggcga aaaaatttca ggtcctcctt tgctagatga taatgaggaa
     5641 actaagattt taccaacctc ttcttcccgt tttggttatg ccaatcctaa ggatcatggt
     5701 tttagccagg aaaccttgaa gattcaggtc gaaccatcta ttcataaaag ccgtcgtatt
     5761 gaaaatacca agagaaatgt cttcaattct aagttgaata aaatcttatt tgcggtcatc
     5821 tttctcttga ttttgcttgt tttagcaatg aaacttttgt aatagaaaag gaattgaaat
     5881 gaaaatagga attattgctg ctatgccaga agaactggct tatctggtcc agcatttaga
     5941 taatacccag gagcaagttg ttttggggaa tacctatcat acaggaacca ttgcttctca
     6001 tgaagtcgtt cttgtagaaa gtggaattgg taaggtcatg tctgctatga gtgtggcgat
     6061 tttggctgat catttccagg tggatgccct tattaatacg ggttcagctg gggcagtagc
     6121 agaaggtatc gctgttgggg atgtcgtgat tgctgacaaa ttagcctatc atgacgtgga
     6181 tgtcacagct tttggctatg cttatggaca aatggcgcaa caaccgcttt atttcgaatc
     6241 agacaaaacc tttgttgctc aaatccaaga gagtttatct caattggacc aaaactggca
     6301 tcttggtttg attgctacag gagatagttt tgttgcagga aatgacaaga tagaagcgat
     6361 taagtcccat ttcccagaag ttttagccgt ggagatggag ggggcagcta ttgctcaagc
     6421 agcgcatgcc ctcaatctcc cagtcttagt catcagagct atgagtgaca atgccaacca
     6481 tgaagcaaac atcttttttg atgagtttat tatcgaagct ggacgtcgct ctgcccaagt
     6541 cttgttgacc tttttgaagg ctttagatta agcggaaatt tgacagtttt tctagcttat
     6601 gataagattt aagtaaagaa aagctagaaa acgtttcaga ggatattatg agtattgaaa
     6661 tgaccgtcag tgagattgca gaggtcttag gattatctcg ccaagcaatc aataaccgtg
     6721 tcaaagaatt accagaagaa gacacagata aaaatgacaa aggggtaaca gttgttacca
     6781 gaagtggctt gattaagcta gaagaaatct ataaaaaaac gatttttgaa gatgagcctg
     6841 tcagtgaaga tgtcaaacaa cgtgaactga tggagattct agtggatgag aagaatgcag
     6901 aaattcttcg tctttatgaa caattaaaag ccaaggatcg tcagttatca gaaaaagacg
     6961 agcagatgcg tatcaaagac cgtcagattg ctgagaaaga caaacaatta gatcagcaac
     7021 aacaattgac ccttcaagcc atgaaggatc aagaaaatct taagctagag ctggaccaag
     7081 caaaagaaga agtccaatcc actaagaaag gcttttttgc tcgtttattt ggaggataat
     7141 caaggagctg tttaggttaa atgctaacct gatggcttct tttgtttgtg aatagtatag
     7201 ttgattagaa cagagacagt agaggagagg tacagaatgg aacgattaga agattacatt
     7261 gcttttgact tagaatttaa tcagcacgaa ggacaaacac acttgattca agtatcggca
     7321 gtgcgtttta gagatggtcg ggaaatcgat gcctatgatt cctatgttca taccagcgtc
     7381 cctctaaaaa gctttatcaa tggtttgacc gggattacag ctgagacctt aaaagatgct
     7441 cctccagttg agaaagtctt agaagaattc caagagtttg tgggttcttt gctgatgata
     7501 ggctacaatg ctgctaaaag tgatttacct attttagcag agcatggttt agactatagc
     7561 caacagtaca aggtggattt gtatgatgaa gcttttgaac gtcggagttc agatttacat
     7621 ggtattgcca atctcaaatt gcaaactgtt gcctcatttc ttggatttca aggtcagtct
     7681 cataatagtt tagaggatgc tcggatgacg gcgcgtgttt acgaatcctt cttagagtcg
     7741 gatcaagcta gggaattgtt agagacagaa agtagcttat caaacaatcc ttttggtgtc
     7801 ttggacttgt ctcaattatt tgactaggag tgcctatgaa acctgaaaaa cctaaaaaat
     7861 tgggtcttaa acagatttac ctcaatcttg ataaaatcct ctttctattt ttcttgattt
     7921 ttatgatagt ggagtatttg tgggtaccat tcaattcatg gttggctagc ttcttattag
     7981 atcagacagg ttatcttttt atttcctata acaatgtctt agccatcctt actcattctc
     8041 cattgattag tctagctttt ttagccttga ttgtaattaa ccttctggtt gcttattttc
     8101 aaataggact cctctttatt ggtgctcgtc atctcctcta taatgaaaag cgaaggctaa
     8161 ttgagtatat ccgcaaggtt ttccatgaaa gttttggatt tatgaaaaag ctaacttgcg
     8221 ctaaagcctt gtttatcttt ttctatatag ctatgctttt tccttttata cggaaaatgc
     8281 tgaagattta ttatcttaat aaaatcgtca ttccagagtt tatccaagct tatttagaag
     8341 ataaatattg gatgtggtgg ctgagcatcc tacttctatc tcttatcttt ctctatgtct
     8401 ctgtcagatt gatgtttgcc cttccgaaga ttgtatatga tcagctgact gttcgagaag
     8461 cagtgatgtt tagcttggag aaaaccaaga agagagttac tttttatgct tggaatttat
     8521 tttacatctt gctcaaagca catttattat tttatcttcc tttgattcct ttattattgg
     8581 ctcaaacttt ggtagatgat atcactcaga gagagtctct gattcttggt attttgaatt
     8641 ttgtcgtgat taaaaatctc tattatatgg ctctgactta ttttttggtt aaatttgttt
     8701 catttttgac aggaaaggag ctggatatgc ttcctaggag agaaaaagat cacatcgtgc
     8761 gatggggtgt catgacttgt gccagtcttt tctttgcctt agaaggttat atttatctgg
     8821 aggctcccat ggttcatcta cctcaactta tttctcaccg aggggtttcc aatgcaaatg
     8881 ggattcaaaa tacagtagag tccttggaaa ctacagcaca actcaaacca gacttggtgg
     8941 agacggacgt gcaggaaaca aaagatgggc agtttgtcat gatgcatgat gctaacttga
     9001 aaaatctagc aggtatcaat aaaagtcctc aagacttaaa cttggaggag cttaaaggga
     9061 ttgatatttt tgaaaatggc taccagacta aaatttcaag ctttgaagat tatctcagtc
     9121 gagccaacga acttggtcaa aaattactaa ttgaaattaa aaccagtaaa aaagatagtc
     9181 cagacatgat gaaccgcttt ttagcccgtt atgctgcaaa gctcaagatt tatggacatc
     9241 aaatccagtc tttagactac catgttgtcg aaaaagtaag acagtatgat gccgaactgc
     9301 cagtttattt catcatgccc tacaattctg tctttcctaa aacaagagcg acaggatata
     9361 ctatggagta ctcaaccttg gatgaatatt ttgtaagcaa actatggaca acggatcaga
     9421 aactttatgt ctggactgtc aatgattcag aagctatcag caaatctctt aacttaggag
     9481 tagatggagt gattacagat gatttggaaa aagttcagcg ggaaatagaa gtagcccaag
     9541 aagatccaga atatacggat ttgctcttga aaaaagcatt ggaattcttt gaattttagt
     9601 aataaaaaag aggaacggtt gttcctccca tgataggtaa gtattcttgt gaatgcttgc
     9661 ttttttgtgg ttgcaatccg tttacgagaa ttgtttgtat tgttcttgtt tcaatttact
     9721 ttatagtgct ttgaagttgg aatgctacac tataacttct
//
LOCUS       DAVBCW010000072         9731 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVBCW010000072 DAVBCW010000000
VERSION     DAVBCW010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9731
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.072"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(420..1556)
                     /gene="dnaJ"
                     /locus_tag="VQ514_001488"
     CDS             complement(420..1556)
                     /gene="dnaJ"
                     /locus_tag="VQ514_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET2500235.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(1761..2114)
                     /locus_tag="VQ514_001489"
     CDS             complement(1761..2114)
                     /locus_tag="VQ514_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500236.1"
                     /translation="MSVNKNNLSDIADYHEKIKFFEELFNLDYNPTEMLVTVERNGNV
                     IYKEFTEVAVGKSKYDHTSVFKDVMIYFSDHICQELHLYKTQDPHYNKFEFDLAKRSL
                     KITTRDNTTIFISNE"
     gene            complement(2127..3950)
                     /gene="dnaK"
                     /locus_tag="VQ514_001490"
     CDS             complement(2127..3950)
                     /gene="dnaK"
                     /locus_tag="VQ514_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HET2500237.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(4430..4954)
                     /gene="grpE"
                     /locus_tag="VQ514_001491"
     CDS             complement(4430..4954)
                     /gene="grpE"
                     /locus_tag="VQ514_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HET2500238.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(4981..6015)
                     /gene="hrcA"
                     /locus_tag="VQ514_001492"
     CDS             complement(4981..6015)
                     /gene="hrcA"
                     /locus_tag="VQ514_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HET2500239.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFHRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTGLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVNLLNFANLEAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQVNVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(6179..6688)
                     /locus_tag="VQ514_001493"
     CDS             complement(6179..6688)
                     /locus_tag="VQ514_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HET2500240.1"
                     /translation="MNSRVEFRIFTIVDLDKEEEYLHEMHLKGWRYRTSRFGLFYFDQ
                     CQPDDVIYRIYDSRFLKKYKHELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTTTFLGGLVVCTSLFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
     gene            7296..9233
                     /locus_tag="VQ514_001494"
                     /pseudo
     CDS             7296..9233
                     /locus_tag="VQ514_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            9231..9731
                     /locus_tag="VQ514_001495"
                     /pseudo
     CDS             9231..9731
                     /locus_tag="VQ514_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819352.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         2862 a         1983 c         1896 g         2990 t
ORIGIN      
        1 ccatgacaag ccgacgctga cgtggtttga agaaattttt gaagagtatc attcaccatt
       61 tcacaactaa atcgaagact ttctgccgta atgaaaacac ctgaaacagc ttggtttcag
      121 ctgtccggaa actttaagac ttaggttcaa agtttaggta tggaacttcg aaggaggtcg
      181 ctaccgtccg tcattactta ggaaagtctt aaaaaatcta taaagtaaaa agagcccgac
      241 agctcttttt gtttatactc aatgaaaatc aaaaagcaaa ctaggaaact agccacgggc
      301 tgctcaaaac actgttttga ggttgtagat acgactcaca tatatacggc aagccgacgc
      361 tgacgtggtt tgaagagatt ttcgacgctg acgtggtttg aagagatttt caaagagtat
      421 tattctccat caaaggcatc tttaatatgg tcaaagaagc cttttttctt tggatttact
      481 ttcaagtcac cagcagccgc gaattctttc aaggctactt tttggcggtc gttcaagcct
      541 gtcggtgtta cgacattaac agtaacgtat tggtcaccaa ctgcaccgcc acgaaggctc
      601 ggtgccccct tactacgtag gcggaatttc ttaccagtct gagttccctc tggaataacc
      661 aattcaacat caccgtgaac agttggaata tctactgtat caccaagagc cgcttggaca
      721 aagttgaggt tgagattgta gaagatagtc gttccttcac gttcaaactt gtcgctagct
      781 tctacagaaa ctactacata caagtcccca taaggtccac cgttaaagcc tgcttcgcct
      841 tgaccagcga ggcgaatttg ttgacctgtt tccacaccag cagggatttt cacatgtacg
      901 ctatgagctt gtttctcatg acctgttcca tgacaggttg tacatggata tttgatttct
      961 tttcctcgac cgtgacagac atcacaggtt acttggcgac gcatcatacc aagaggagtc
     1021 tgcgtatcga cgttaatgac accagcgcca tgacagcgtc cacaagtgac tggacttgtc
     1081 cctggcttag caccagagcc attacatgta cgacagccag cttcacgatg atacttaact
     1141 tccttctcag ttccgaagat agcttcttca aaggtcaaat tgacacgata ctggagatca
     1201 tctccttggc gaggagcgtt tggattgcgc gaagaaccgc ctccgccgaa gaaacttgaa
     1261 aaaatatcct caaaaccacc gaagccacct gccccattga aaccaccgaa accaccagct
     1321 ccaccaaaac caccattggc gcctgcagca ccatactggt cataggcagc acgtttttgg
     1381 tcgtcactca aagtctcata ggcttcttga acttccttgt acttgtcctc agcaccaggc
     1441 tccttgttga tatctgggtg atattttttg gaaagcttac gataagcctt tttgatctcg
     1501 tctgccgaag cgttttttga cacccccaga cgatcataaa attcagtatt gttcatacaa
     1561 gataccaaga ccgtaaaatt cgactgaaaa ataggaaatc tgacgctgga gcgacgctcc
     1621 tagacagatt tctctttttt ccgagaattt aggtcgggtt caattccttt cttttatatt
     1681 gatgagataa tactccagcc actcggccct atcccttttt accgaaaaac agaggctgag
     1741 ttgcaacctc tgtggttaga ttattcattt gaaataaaaa tggttgtatt atctctagta
     1801 gtaatcttca aagacctttt agctaaatca aactcaaatt tattatagtg tgggtcttgt
     1861 gttttatata ggtgtaactc ctgacatata tgatccgaaa agtaaatcat aacatcttta
     1921 aacacagaag tgtggtcata tttactctta ccaacagcta cttctgtaaa ttctttataa
     1981 atgacatttc catttctttc cacagtcacc agcatttctg ttggattata atccaaatta
     2041 aataattctt caaaaaactt tatcttttca tgataatctg caatgtcaga aagattattt
     2101 ttattaacac tcataacact agccccttat ttttcagtaa actctccgtc tacgacgtca
     2161 tcgcctgcgt ttcctgttgc ttgtgcgcct tctgctcctt cttgagcttg ttgcgctgct
     2221 gcagcttgtt cgtagagttt aacagcaagt ccttgagctt tttcgttcaa tgcttcaagt
     2281 tttgctttca tgtcgtccaa gttgttgtct tcttgagctt tcttaaggtc atcaagggca
     2341 gcttgggcag cgtcacgttc tgcgtcgaag cctttacctt cagtttcctt gattgtcttt
     2401 tcagtcgcaa agattgcttg gtctacttca ttacgaaggt ctacttcttc tttacgtttc
     2461 ttatcggctt cagcgtttgc ttctgcatct ttcatcatgc ggtcgatttc ttcgtcagtc
     2521 aaacctgagt tcgattggat gacaatagtt tgttcttttt gagttccaag gtctttggcc
     2581 ttaacagaca cgataccgtt cttgtcgatg tcaaatgtta cttcgatttg aggaattcca
     2641 cgaggtgcag ctgggatatc agtcaattgg aagcgtccaa gagtcttgtt atctgctgcc
     2701 attgggcgtt caccttgaag aacgtggata tcaacggctg gttggttgtc tgctgctgtt
     2761 gagaagactt gtgatttaga tgttgggatt gtagtgttgc gatcgataag ttttgtaaat
     2821 actccaccca ttgtttcgat accaagtgac aatggcgtta catcaagaag gacaacgtcc
     2881 ttgacatcac cagtaatcac accaccttgg atagccgcac ccatagcaac tacttcatca
     2941 gggtttactg atttgtttgg ttctttacca gtttcagctt taacagcttc aacaacggca
     3001 gggatacgag ttgaaccacc aacaaggata acttcgtcga tttctgacaa gctcaaacct
     3061 gcatctgaaa gggcttgacg aactggaact tttgtacgtt caacaaggtc acgagtcaaa
     3121 tcgtcaaatt tcgcacgagt caaagtcatt tccaagtgaa gaggtccagc ctcacctgca
     3181 gtgataaatg gcaagctgat ttgtgttgaa gttacaccag aaaggtcttt cttcgctttt
     3241 tcagccgcat ctttcaaacg ttgcattgcc atcttgtcag tagacaagtc gataccgttt
     3301 tctttcttga attctgctac caagtggtca atgatttttt ggtcaaagtc gtcaccacca
     3361 agtttgttgt cccctgcagt tgacaatacg tcgaagacac cgtcacccaa ttcaaggata
     3421 gagacgtcga atgtaccacc accaaggtca aataccaaga ttttttcttc tttgtcagtc
     3481 ttgtccaaac cataagcaag agctgctgca gttggttcgt taacaatacg ttctacttca
     3541 agaccagcaa ttttaccagc gtcttttgtt gcttgacgtt gagcgtcgtt gaagtaagcc
     3601 ggaactgtga taacagcttt ggttactttc tcaccaaggt agtcttcagc gtagcctttc
     3661 aagtattgaa ggatcatagc tgagatttct tgtggagtgt attcttttcc atttgcagaa
     3721 actttttcag aagttcccat cttagatttg atagagataa ctgtatctgg gtttgtaact
     3781 gcttgacgtt ttgcagcatc accaacgatg atttctccgt ttttgaatga gactacagat
     3841 ggagttgtgc ggtttccttc tgggtttgcg atgattttgc tttcagttcc ttcaagaact
     3901 gcaactgctg agtttgttgt acctaagtca ataccgataa ttttagacat gtgtttttct
     3961 ccttgttagt taattttcta tttttctttt tgatactctt cttaagtggc gacgccgtca
     4021 gagcttgact tcgtcaatct ctttgactaa actttgagcc taaggtctca aaatttgcgc
     4081 aaatagcacc acggcgaaga gtatcgtctt tggttcgctt cgctcactat tacaaagcca
     4141 aacatatttt atctttcttc atagtttatt gtcgtttcgg acaagttttc tattatgtaa
     4201 attgcgacac gaggagtcga aatcgatttt atttcgacga cgagttagta aggaagctag
     4261 gcaaacgtca tagcgattgc cgttttctga cgagttgctt tagctaccgt cagaattgcc
     4321 gaatcgaaca cgccctaagc tctgagtaaa aaagatagat tttcttgtgt tcatcgaaca
     4381 cttcgtcaat ctcctatttt tactgcgagc tttttacgag ctttgtatct tagttataca
     4441 ccactaccat tgctgggcgt aggatgcggt catggagttt gtagcctttt tgaaagactt
     4501 gggcgatggt atctactggg tgttcatcgt ctgctgggag agtttggatg gccatatggt
     4561 agttatggtc aaattcgcca tctgctgcga tttcttcaat tccttcttct ttcaaagcgt
     4621 gaatcaagct ttcttgcacc atccccaagc ccttcttcac atcatctgtc aaaccttcaa
     4681 ctgcaagtgc acgctcaagg ttgtcaagag atggtaagat tgcttttgcc aagtcctggc
     4741 tacgataacg ttgcaagttt tgacgttctt cattggcacg gcgttggata ttttgcattt
     4801 ctgcatgagc gcgaagatat ttgttttcga actcatctgc acgttcattt gccaagtcca
     4861 actcagactt ttcaggagtt gtttcttcag ctgtttccac aacttcctct tcttgaactt
     4921 cttctacttc ttcatttttt atatcttggg ccatgttcgc ctcctttaat gatttcaatc
     4981 ttaatgtact tcgtaatgat tactgctgag gtagcggtaa aaatctgtca acttcatggt
     5041 caaaacacgg ttgaccacat tgacttgatt gattagctgt tggtaatcca gattaactgg
     5101 accgataatg gctagaattc caactccccg ataaggaatg aggaacttac tactaatcac
     5161 cgctaggtca gctaaacagg actcttgacc gtctgcaaca cgaacatttt gcatctgatc
     5221 ctcacgcaac ccctcacgaa tctccaaggc caccttttgc ggttggtcaa agaactgata
     5281 ggcttctaga ttggcaaaat tcaagagatt gaccttgccc gccatcacaa tgttttcgtt
     5341 gaacatttcc ttaaagatgt gttcaaagag atcgatgaca ttatccgttg ttgtaaagta
     5401 acgctggata atctgcggaa tctccgtccg aatcttgtag tgaatatcta aaacggtgtg
     5461 accgaggaaa cgttcctgaa tgatgctctt cagtttcagc aaatcctcct gcaagaagtt
     5521 ccttggaatc agaaactgac tagtaaccgt tcgcgactcg tctagggtaa ataccgccaa
     5581 ggctgtatgt tgccccaaaa caacgatatc aaaggctgtc aaacgttgcc tgctcggctc
     5641 aacatccagt gccactaccg tacagccact caggcctgtt agcaagttag cagcctcttg
     5701 cagaatatcc tccaatttga agaattcctg atcaaaggct ttgacaatct catatacctc
     5761 attttcagcc agccggtgaa aatccagtga gtgtttcaca tagtactgaa aaccagcaac
     5821 acttggcatc cgaccgcttg aagtatgagc cttctcaagc aacccttgtt tttctagttc
     5881 cgccatgtca ttacgaatgg ttgcactgct agagttaata gactcttgca aggcttttga
     5941 tccgacaggt tcgtgcgttt tggtaaagat gtcaataatc agatttaaaa tatcctgctg
     6001 acgctctgta accatctcat cactcctctc tgtctgatca attagcactc aatgcatcca
     6061 agtgctaact tatgattcta ttatacaccc ttaaaagaga atgtcaagac aaaaactcaa
     6121 aaaattagca ctcttttatc aagagtgcta aaaatcaatc tgaagaccta gaaccttgct
     6181 attcatctac tcggtatttc ctttttagtc tgaaataacc atgaattaga taagaaatca
     6241 gaaaagcata taaaacaaaa ataagaaaga aagacataga aagatcctct ctaaacaaac
     6301 tcgtacaaac aacaagacca cctaagaaag tagttgtaca ggaacgaagt ctagatcgca
     6361 taacttccca tttgagcccc ttactcatat agacttgatc ctctggaagt aaatcagaag
     6421 acgatttacg aagaatcgaa caagaacctg ctcctatcaa ttcccaacct ctatctctaa
     6481 aatcttgcag ttcatgctta tattttttaa gaaatctaga atcatagata cggtagatga
     6541 catcgtctgg ttgacattgg tcaaaataga acaaaccaaa acgactcgtt ctatacctcc
     6601 aacctttcaa atgcatctca tgtaaatatt cttcttcctt gtccaaatca acaatggtga
     6661 aaatccgaaa ttctactctg ctattcattg tcttacccca aaattagaaa acatgcctgg
     6721 cgttatttat tagataattc tttccacttt tgactcaatc tccaaaaaat ataagaaatc
     6781 tgaatcgcaa aaactatcaa taaaacccaa tctattatga aaatcaaaaa cactttccaa
     6841 ctgaaagaac tacctccagt gacaaacttt gagcaaaacg gtagtagagc taaaaagaga
     6901 agtaaaatag gaagcatccg cattgttaaa atccgtttgg cataaaaaaa tctttattta
     6961 aactaaaata ttatagcaaa atttacgcca gtttttgaac ggctgatgta gatattttat
     7021 actttcaaaa tgtttaaatg tgattattta tttttgaaaa atagctcacc agcccgactg
     7081 aaagtgctta tagaatgata ataagtcgcc tgccgaaaac agcggaaaat agcggtgtta
     7141 tgcggagata atctgacgcg atgcgaaagt atattgcata cttattttca acaatttagc
     7201 agagtatttt tataagtgtg atataataga agtataattt gttctgatag tttattttat
     7261 ggagaagtag atttttagaa tgcggagggt tcaatatggt tgagtttata aagtctaaga
     7321 aagaaatgag tgaggaggat attaaagcaa atttcatcac tcctgctatt gtatccaaag
     7381 gatggaaaaa tggtgagcat atcgcttacg aagaatactt cactgatggt cgaattgagg
     7441 ttagaggaga taaggctcgt cgtaaagaag gaaaaaaatc agactattca ctgtattacc
     7501 aatttggaac tcgaattgca attgttgagg caaaggataa taaacacagc gttcgagcag
     7561 gattacaaca agctattgaa tatggagaga ttttagatgt tccatttgtt tattcttcga
     7621 atggtgatgg ctttattgaa cacgaccgta tcacgagaga agaacgtgag ctggagttag
     7681 acgaattccc tactcgtgaa gaattatttt ctcgtatgac gaaggaaaaa ggattgacgt
     7741 acgaaattac agaagctatc tcaactccat actatacaga cgccttctca atgaaaacgc
     7801 cacgctatta tcagcaaata gctatcaacc gtactattga aacagttgcc agaggacaaa
     7861 aacgagtaat gtttgtgatg gcaacaggaa cagggaaaac gttcatggct tttcaaatta
     7921 ttcatcgcct tcgaaaagct ggtttggcta aacgagtttt attcttagca gatagaaaca
     7981 tcttagtaga ccaaacgatg gctgaagact ttaagccatt cgaaaaggta atgacgaaaa
     8041 ttacaccaaa acttttgact gctcctgaaa aattaaattc tttcgaaatt tatctagggc
     8101 tttatcagca actaactggt gaagatggaa ctgaaacaca ttatcaaaaa tttgacaaag
     8161 acttctttga tttaatcgta attgatgaag cgcaccgtgg ttcagctaag gaaaacagta
     8221 actggcgtaa ggtaattgat tatttcagtt ctgcgacaca gattgggatg accgctactc
     8281 ctaaagaaac caagaatgct tccaatacgg aatactttgg taagccaatc tatacttata
     8341 gtttaaaaca gggaatcgag gatggttttt tggctccata tcgtgttatg agggttaatt
     8401 tagatgtgga tgtggatggt tatcgtccag aaactggaaa agttgatgct aacggacaat
     8461 taatagaaga taggtactac ggcaggaaag attttgataa aaccattgtc attgatgata
     8521 gaacgcaaag agttgccaag tttgtttctg attatatgaa gcaaaacaat gcacgatttg
     8581 ataaaacaat tgttttttgt gttgatattg accatgccga gcgaatgcgt gctgcacttg
     8641 taaaagagaa tctagactta gtccaagaag actatcgtta tgtcatgcaa gtaactggtg
     8701 acaacgctga aggaaaagct caactggata actttatgga tgtcaattct aattttcccg
     8761 ctattgtaac aacgtctaaa ttattaacga caggagttaa tgctaaaaca tgtcgtttga
     8821 ttgttttaga ctctaatatc caatccatga ctgaatttaa acaaattatt ggtcgtggca
     8881 cacgtcttta tcctcaaaag gggaaagaat tttttacgat tattgatttt cgaaatgtta
     8941 ccaatttgtt tgctgaccct gattttgatg gtgagccagt gaaggtgctg gaaacaggtg
     9001 cgaaaacagt cagtggttct acgcctggtt tcgtaggtga ggaaggtgac ccagtagaaa
     9061 aatatatcgt tacagacaag caggttacca ttcttaattc tactgttcaa gtattggatg
     9121 aaaacgggaa actgattacc gaaagcctga ccgactacac tcgaaagaat atcttaggta
     9181 gctacgccac tttgaacgat tttatcacag tttggcatac ggcagataag aaacattatg
     9241 ggaagtttgc tgatggaagc actcaagaaa ttgatgttcc ttatgatatt cctgatactt
     9301 gggagtgggt gaggttttct acattggttg aaattgtcag aggtggctct ccacgaccaa
     9361 tcaaagatta tcttacttct gaagtagatg gaataaattg gataaaaata ggtgatactg
     9421 aaaagggtga aaagtatata aataatgtta aagaaaaaat caaaaaatca gggcttaaca
     9481 aaactagatt tgtaaaaaaa ggtacatttt tgttaactaa ttctatgagt tttggtagac
     9541 cttatatttt gaatgttgat ggtgcaatac acgatggatg gttggctatt tcgaactatg
     9601 aaaactcatt aaataaagat tacctattct atattctttc atcaaatgta gtttattctc
     9661 aatttctatc tctaattagt ggagctgttg tgaaaaactt gaatagtgat aaagttgctt
     9721 ctattcttat c
//
LOCUS       DAVBCW010000073         9691 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVBCW010000073 DAVBCW010000000
VERSION     DAVBCW010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9691
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.073"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            298..558
                     /locus_tag="VQ514_001496"
     CDS             298..558
                     /locus_tag="VQ514_001496"
                     /inference="COORDINATES: protein motif:HMM:NF012235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2500241.1"
                     /translation="MSGGETKRVALAKIILKNPPLIFADELTASLDSTTSQGIMKLLL
                     GMKNKNRLIIIATHNPDIWNQADEVINLNQLLQSIYIFYILK"
     gene            complement(662..1132)
                     /locus_tag="VQ514_001497"
     CDS             complement(662..1132)
                     /locus_tag="VQ514_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET2500242.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQTLLAENDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(1149..3422)
                     /locus_tag="VQ514_001498"
     CDS             complement(1149..3422)
                     /locus_tag="VQ514_001498"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HET2500243.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLASDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
     gene            3769..6870
                     /locus_tag="VQ514_001499"
     CDS             3769..6870
                     /locus_tag="VQ514_001499"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HET2500244.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQGVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQERFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     ETEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRLAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNSSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIENRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEVREGAFYYIKGKVQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSDELEAKLNEIVMKT
                     IYR"
     gene            6952..7959
                     /gene="pfkA"
                     /locus_tag="VQ514_001500"
     CDS             6952..7959
                     /gene="pfkA"
                     /locus_tag="VQ514_001500"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HET2500245.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            8016..9521
                     /gene="pyk"
                     /locus_tag="VQ514_001501"
     CDS             8016..9521
                     /gene="pyk"
                     /locus_tag="VQ514_001501"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HET2500246.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
BASE COUNT         2984 a         1679 c         2123 g         2905 t
ORIGIN      
        1 ttgttttgtc gcttttcatt ataggtcata tgggactttt tttctacaat aaaataggct
       61 ccataatatc tatagtggat ttacccacta caaatattat agaacccttt tttattgaca
      121 gtatcaggat atctttttca aatttttggt ttgttagata atgaaaatgt ttctatcaat
      181 ctagatttag gattaataaa tcgtaaatgt aattataaag aaaaagataa gttaaaaaaa
      241 gatgtattag caagagttgg tctaactcat attcatctat aacaaaagat atatgaattg
      301 tctggcggtg aaacaaaacg tgttgcttta gcgaaaatca ttcttaaaaa tccaccatta
      361 atattcgcgg atgagttgac tgcatcactc gattcgacga cttcacaagg aattatgaaa
      421 ttgttattag gtatgaaaaa taaaaatcgt ctaatcatca ttgcaactca taatcccgat
      481 atttggaatc aagcagatga agttataaat ttaaatcaat tattacagtc aatatatatc
      541 ttctatattt taaagtaatt ttatctgaat ccgttaagag actattgtcc caggctcttt
      601 ctgatgttaa agactttcta ccaggttttt taaaagcata attgttagtt gtagtcattt
      661 attattcttc aaagaaaaat ggtggggcga attttttcag ttcttcaaag cacttttgag
      721 cagtatctgc atcttcacag atgataagac agacatcatt accacaaagg gtagcgatag
      781 cgtcagggaa gctcaaagta tcaatgatag aaccaaagga ttgagccagt ccaggaaggg
      841 tttttagtag gacttggtgt tgaactgggc gcatccagac aagggcgtct tccatgtaga
      901 gttcgagacg tttttcccat tttgagatgg aaccattgtt aagaacataa taagcgctat
      961 cttcttcgcg gacttttgat aggttcatat ttttgatgtc gcgtgagagg gttgcctgag
     1021 ttacttgaat gtcgttctca gcaagaaggg tttgcaactc agcctgtgta tgaatcttgt
     1081 tttttgtgat aagagcgcgt ataagttggt ggcggtgttc tgatttattc ataataatgt
     1141 aactcctttt agcaaggtaa ggtaagcatg gactgagcga ggtcgacagt caagtggtag
     1201 tctgtattgt cacggatggt gatttcaaag tcagtagtat agaggactaa acggagagtg
     1261 tctccttctt ttagcttgta aatagttggc tgcagttcaa attgaacgtc catccattca
     1321 tctgcagtaa tatcctctac taacagtaaa tcatttctat tttgtaaatt aaggtaacct
     1381 tttgtcacga ctctttgtgc ctctggtcta aatggcaatt cacagagatt ttccaacatg
     1441 tgatagcgac cgttgtcaat ggttctagca cttaaaatag ctggataagg ttgtaggtat
     1501 ttcttttgcc caaattctag cagttgggca gataagagcc ccttgtttgt actggatttg
     1561 atacgaagat tgagctgagc gcgaccgttt aggtggagat ctttagtcac aggaaggtta
     1621 atagtaatct gattagcttt cccttgatag agctctgtat tgaaggtttg gtatgtctta
     1681 ccatagcgct caaaatcctt atctgggtac tggttttgaa tagcttgctc ttcttgacca
     1741 agtgagaagg tttcacagtt ttcttgccca ccgaagttat caagtgataa ccaagtctgt
     1801 ggagctgtat tgtcctgcca gataacagta ggaagttgaa agtctgtttc ctgtcctagt
     1861 aatttcttgg tcaataaggc atttatggac tcacggaagt caattgattg ccaattgttc
     1921 atgtaaacat gggcaccatt atggaaaaag agatgcttgt gtatatgagt aggaagagca
     1981 tggaacatct ggtaaacatg aagtggtttg acattccaat cctgagaacc atgagtaaag
     2041 acaacctctg cctttacttt atgggcattg agcagataat tgcggtcatg ccaaaactga
     2101 ttgtagtccc cagtttttcg gtctagttga gctttcactt tttctaagtc agcttggtga
     2161 gcttcattgc cacggatata gtcgctagcc aagagattac gagaataggt taactcagca
     2221 agggagtcaa agtcctcacc tggataacca cctgggctag tcaccagacc gttttcacgg
     2281 tagtagttgt accatgatga aattcctgcc tcggcaatga taacttctaa accatcgact
     2341 cctgtagtcg caagaccatt ggacatggta cctagatagg aaagtcccgt tgtagcaact
     2401 tttccgtttg accaatcagc cttgacttga cgctggcgcg tgtgatcagt aaaggcacgg
     2461 caacggccgt taagccaatc gatgacattt ttataagcct cgatttgctg gtagtctcca
     2521 ttagtcatga aacctgtcga gtctttggta ccaacacctg agacatagag attggcaaag
     2581 cctctcggaa ggaagtagtc gtttagtgta tagctagagt tgatgtgagt tagcttttcc
     2641 tcagcctctg ctataagctc agctttacct tggggttgga cgagatttag ttgaggtttc
     2701 tctagctcaa tcttgtgagg aagtttaacc tcaagctcgc cctccatctt gtagagagcc
     2761 ttgtcactag ccttgtcatt ggttccctga tgataagggc tggctgtcat gatggcaggg
     2821 atttttccat caaaacgagg gcgaataatg ctaaccttta ctaggtctga tagccctttt
     2881 tggtcagtat cgacacgaga ctcaacgtaa acgacttcac gaatgacatc ctggttagaa
     2941 aaagtagcca aactcttgcc gttaaagtag tggtagtcat tatcctccgg aataagacca
     3001 tcactaacaa gttggtcgat aagagtattt ccttttttgg tgcgagtatt gagtaactga
     3061 tagagatttt caatcaagtc accatatata atgggaaatc cagtttcttt acgaaaaacg
     3121 tcactatctt cgaagtcaac caaataagaa aagcctaaaa gttgaaaagc aacagtataa
     3181 aaaatatctg ctgtcagttc atcttctgat tgaaaaaatg tcagcaggtc tgttttttta
     3241 tcagctgcta ggatagaaag tgggtagttg gtgtcttgat aagtgaaaaa gaaacgacgt
     3301 aaaaaggttt caagtgagtc tttgtgattg gctgtatttt gtaaatcaaa gccacatttt
     3361 tttagttcag ataagacatt ttcttttgga aaattgatat aactatattg attaaaacgc
     3421 atagaacctc catatagaat gacagttaag gttattatat caaaaaaaaa gcagaaaggg
     3481 aattgttaac ttcaaaagga aataatccaa taaaaatgaa taaagtacta aattcaatat
     3541 agagaacaga gtaacaataa gaataaatag atagggtata aaagttctag gagatttata
     3601 ttatatgctt tctattttta tatacaatat agtataaata taaaaatgat gacaaaaata
     3661 caaatgaata gaaaataaat tagtaagctg atgaaatttt tctcaagaga agccatttat
     3721 aggtgaaaat ggtataatat agtgagaagg atagaggaga agtgtaaatt gatcgcacaa
     3781 ctagatacaa aaacagtcta tagttttatg gaaagcgtca tttcgatcga aaagtatgtg
     3841 agagcagcta aagaatacgg ctacactcat ttggctatga tggatattga caatctttat
     3901 ggcgctttcg actttctaga gattacaaaa aaatacggca ttcatccttt gctagggctt
     3961 gaaatgacag tgtttgtaga tgatcaggga gtgaatttgc gctttttagc tctatctagt
     4021 gtgggctatc agcagttgat gaagctttcg acagccaaga tgcaggggga gaaaacttgg
     4081 tcagtcctgt cccagtacct ggaggatatc gcggtcattg tgccttattt tgatagagtt
     4141 gagtcgttag aactaggctg tgattactat ataggggttt atccagaaac actagcaagc
     4201 gaatttcatc atcctatctt acctctttat cgggtcaacg cttttgaaag cagggataga
     4261 gaagttcttc aagttttaac agcgattaaa gaaaatctac cgctcagaga agttcccttg
     4321 cgttcgagac aagatgtctt tatatcagca agttctttag agaaactatt ccaagagcgt
     4381 tttccgcaag ctttggacaa tttagaaaag cttatttcag gcatttctta cgacttggat
     4441 actagtctga aactgcctcg ttttaatcca gctagaccag cagtagagga gttgagagag
     4501 cgtgctgaac tggggcttgt tcagaagggg ttgactagta aagaatatca agatagacta
     4561 gaccaagaat tgtctgttat tcatgatatg ggctttgatg attatttctt ggttgtttgg
     4621 gatttgttgc gttttggacg atcgaatggc tattatatgg gaatgggaag gggttctgca
     4681 gtaggcagtt tggtttctta tgccttagac atcacgggga ttgacccagt agagaaaaat
     4741 ctgatttttg aacgctttct taatcgtgaa cgctatacca tgcctgatat tgatattgat
     4801 atcccagata tttatcgtcc agattttatc agatatgttg gtaataaata tggtagtaaa
     4861 catgcggcac aaatcgttac tttttcaacc tttggagcca agcaagctct tcgagatgtc
     4921 ttgaaacgct ttggtgtgcc agagtatgaa ttatctgcaa ttactaagaa aatcagtttt
     4981 cgtgacaatc ttaagtcggc ctatgagggc aatctccagt ttcgtcagca aatcaatagt
     5041 aagttagaat accaaaaagc ttttgagatt gcttgcaaga tagagggcta tccaaggcaa
     5101 acctctgtcc atgcggctgg tgttgtaatt agtgaccaag atttaaccaa ctacattcct
     5161 ctaaagtatg gtgatgaaat tccactgact cagtatgatg ctcatggagt tgaggctagc
     5221 ggacttttga agatggactt tctgggacta cgaaatttga cctttgtcca gaagatgcaa
     5281 gagttgcttg ctgaaacaga aggtattcat ctgaaaattg aagaaatcga tttagaagac
     5341 aaagaaacat tagctttatt tgcctctggt aatacaaaag gtatctttca atttgagcaa
     5401 ccaggtgcca ttcgtctgct taagcgtgtg caaccagtct gttttgaaga tgtcgtcgcg
     5461 actacttctc taaatcgacc gggtgctagt gactatatca ataattttgt ggcaagaaag
     5521 catgggcagg aagaagtgac tgttctggat ccagtactgg aggatatttt ggctccaacc
     5581 tacggcataa tgctctatca ggagcaggtt atgcaggttg cccagcgact tgccggattt
     5641 agtcttggga aagccgatat tttgcgtcgg gctatgggga aaaaggatgc ctctgccatg
     5701 catgagatga gggcttcctt tattcaaggt tcattagaag ctggtcatac tgtggaaaaa
     5761 gcagagcagg tctttgatgt tatggagaag tttgcaggtt atggttttaa caggtcacac
     5821 gcctatgcct actcagcctt ggccttccag ttggcttatt tcaaaacgca ttatccagcc
     5881 attttttatc aggtcatgtt aaattcttcc aacagtgatt acttaataga tgcacttgaa
     5941 gcaggttttg aagtagcctc tctatccatc aacaccattc cctatcacga taaaattgcc
     6001 aacaaggcca tctatctagg tttgaaatcc attaaaggag tcagtaatga tttagctctc
     6061 tggattattg aaaatagacc ttattctaac attgaagatt ttatagctaa attacctgag
     6121 aattatctga aacttcctct gctagaacct ttggtaaaag ttggtctttt cgattcattt
     6181 gaaaaaaatc gtcaaaaagt atttaataac ttagctaatc tatttgaatt tgtgaaagag
     6241 ttgggaagtt tgtttggaga tgctatttat agttggcagg aatcggaaga ttggacggaa
     6301 caagaaaaat tttatatgga acaagagctt ttagggatag gtgtcagcaa acatccacta
     6361 caagctattg caagtaaggc tatttacccg attaccccaa tcggaaattt gtcagaaaat
     6421 agctatgcta ttatcttggt tgaagttcag aaaataaaag tgattcgtac caaaaagggt
     6481 gaaaatatgg ccttcttaca ggcagatgat agtaagaaaa aattggatgt tacactcttc
     6541 tcagatttat atcgacaggt tggtcaggaa gtaagagaag gagcattcta ctatatcaaa
     6601 gggaaagtcc aatcacgtga cggtcgtctg caaatgattg cacaagaaat aagagaagca
     6661 gttgctgaac gcttttggat acaggtgaaa aatcatgaat cggatcaaga aatttcacgt
     6721 attttagaac aatttaaagg cccaatccca gtcatcatcc ggtatgaaga ggaacagaaa
     6781 accatcgttt ctccccatca ttttgtagct aaatctgatg aattagaggc gaaattgaat
     6841 gaaatcgtta tgaaaacgat ttatcgctaa aaatacggaa aatagaagaa ttttaaaatc
     6901 aaatgtgata taatcaagaa gaatgttaaa agaaaaagga gcataaccaa tatgaaacgt
     6961 attgctgttt tgactagtgg tggagacgcc cctggtatga acgctgccat ccgtgcagtt
     7021 gttcgtcaag caatttcaga aggaatggaa gtgtttggta tctatgacgg atatgctggt
     7081 atggttgccg gtgaaattca tcccctagat gcagcttcag taggggacat catttctcgt
     7141 ggtggtactt tccttcactc agctcgttac ccagagttcg ctcaacttga agggcaactt
     7201 aaagggattg agcaattgaa aaaacacgga attgaaggtg tagttgttat cggtggtgac
     7261 ggatcttacc acggcgctat gcgtttgact gaacatggct tcccagctat tggtcttcca
     7321 ggtacaatcg ataacgatat cgttggtact gactttacaa tcggttttga cacagcggtt
     7381 actactgcca tggacgctat cgataagatt cgtgatacat catcaagtca ccgtcgtact
     7441 tttgtaatcg aagttatggg acgtaacgct ggtgatatcg ctctttgggc tggtattgca
     7501 actggtgctg atgaaatcat catccctgaa gcaggcttca agatggaaga tatcgtagca
     7561 agcatcaaag ctggttatga atgtggtaaa aaacacaata tcatcgtctt agctgaaggt
     7621 gtgatgtcag cggctgaatt tggtcaaaaa cttaaagaag ctggagatac aagcgacctt
     7681 cgtgtaacag aacttggaca tattcaacgt ggtggttctc caactgcgcg tgaccgtgtt
     7741 ttggcgtcac gtatgggtgc acatgctgtt aaacttctta aagaaggtat cggtggtgtt
     7801 gcggttggta ttcgtaacga aaaaatggtt gaaaatccaa ttcttggtac tgcagaagaa
     7861 ggggcattgt ttagccttac tgcagaaggt aagattgtgg ttaacaaccc gcacaaagcc
     7921 gatattgagc tatctagctt gaataagagc ttgtcataat ttacaattta gttaataaga
     7981 ccaaaaaggt catatataaa ggagtcacaa aaatcatgaa caaacgtgta aagatcgttg
     8041 caactttggg tcctgcggta gaaatccgtg gtggtaaaaa attcggtgag gacggatact
     8101 ggggtgaaaa acttgatgtt gaagcttcag ctaaaaacat tgctaaattg attgaagctg
     8161 gtgctaacac attccgattc aacttctcac acggcgacca ccaagaacaa ggtgagcgta
     8221 tggcaactgt taaacttgcg gaaaaaattg caggtaaaaa agttggtttc cttcttgata
     8281 caaaaggacc tgaaatccgt acagaattgt tcgaaggtga agctaaagaa tattcataca
     8341 aaactggtga aaaaattcgt gttgcaacta aacaaggaat caaatcaact cgtgaagtga
     8401 ttgcgttgaa cgttgctggt gctcttgata tctatgatga tgttgaagtt ggtcgtcaag
     8461 ttttggttga cgatggtaaa cttggtcttc gtgtggttgc taaagatgat gcaactcgtg
     8521 aatttgaagt tgaagttgaa aacgatggta tcatcgctaa acaaaaaggt gtgaacatcc
     8581 ctaacactaa aattcctttc ccagctcttg ctgaacgcga taacgacgat atccgtttcg
     8641 gtcttgaaca aggtatcaac ttcatcgcaa tttcattcgt acgtactgca aaagatgtga
     8701 acgaagttcg tgcaatctgt gaagaaactg gaaacggaca tgttcaatta ttcgctaaaa
     8761 tcgaaaacca acaaggtatc gataacttag acgaaatcat cgaagcagct gatggtatta
     8821 tgattgctcg tggtgatatg ggtatcgaag taccgttcga aatggttcca gtttatcaaa
     8881 aaatgattat caagaaagtc aatgctgcag gtaaagttgt tatcactgca acaaacatgc
     8941 ttgaaacaat gactgaaaaa ccacgtgcaa ctcgttcaga agtatcagac gtattcaacg
     9001 ctgttatcga cggaactgac gctacaatgc tgtcaggcga gtctgcaaac ggtaaatacc
     9061 cactcgagtc agtaactaca atggctacaa tcgacaagaa tgctcaagct cttcttaatg
     9121 aatacggacg tcttgattca gattcatttg agcgtaactc taagacagaa gtaatggctt
     9181 ctgctgttaa agatgctact agctcaatgg atatcaaatt ggttgtaact cttactaaga
     9241 caggtcatac tgcacgtttg atttctaaat accgtccaaa tgctgacatc ttagcattga
     9301 catttgacga attgacagaa cgtggcttga tgttgaactg gggtgttatc ccaatgttga
     9361 cagatgctcc atcttcaact gacgatatgt tcgaaatcgc tgaacgtaaa gcggtagaag
     9421 caggtctcgt tgagtcaggc gatgatatcg ttatcgttgc tggtgtgcca gtaggagaag
     9481 ctgttcgcac aaacacaatg cgtatccgca cagtacgtta agaaaaatat aaaaacctat
     9541 catatccagc tttagagctt gtgtgatagg ctttttgtat agagggtaag aaataggcaa
     9601 aactttcata atggattgat actcttcgaa aatcaaattc aaaccacgtc agcgtcgcct
     9661 taccgtatat atgttactga cttcgtcagt t
//
LOCUS       DAVBCW010000074         9577 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVBCW010000074 DAVBCW010000000
VERSION     DAVBCW010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9577
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.074"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            362..949
                     /locus_tag="VQ514_001502"
     CDS             362..949
                     /locus_tag="VQ514_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500247.1"
                     /translation="MFVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNILSDEVLE
                     VVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVNTIHGNKQ
                     ANTFANFMTGAGISYMIGRIGTLLGGPWGAIIGGAYFGIQAYQSYLDSQSPYPYYITS
                     TYIHVAQKKWKFITEYYRNSNYTGYVKTVTTYVNF"
     gene            complement(1036..1239)
                     /locus_tag="VQ514_001503"
     CDS             complement(1036..1239)
                     /locus_tag="VQ514_001503"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500248.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEDKKQELLNRLNK"
     gene            complement(1536..2240)
                     /locus_tag="VQ514_001504"
     CDS             complement(1536..2240)
                     /locus_tag="VQ514_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HET2500249.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTIGVALKGGLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            complement(2310..4136)
                     /gene="glpO"
                     /locus_tag="VQ514_001505"
     CDS             complement(2310..4136)
                     /gene="glpO"
                     /locus_tag="VQ514_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HET2500250.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKHLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELALSPVDFLLRRTNHMLFMRDSLD
                     SIVEPILDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            complement(4178..5686)
                     /gene="glpK"
                     /locus_tag="VQ514_001506"
     CDS             complement(4178..5686)
                     /gene="glpK"
                     /locus_tag="VQ514_001506"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HET2500251.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            complement(5845..7272)
                     /locus_tag="VQ514_001507"
     CDS             complement(5845..7272)
                     /locus_tag="VQ514_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2500252.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELTIHSEDENLRLSIGAATKGRDIRSLFLDSAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSAGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLAGIRQLLFT"
     gene            7437..8309
                     /gene="hslO"
                     /locus_tag="VQ514_001508"
     CDS             7437..8309
                     /gene="hslO"
                     /locus_tag="VQ514_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HET2500253.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQERHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGAGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            8296..9276
                     /gene="dusB"
                     /locus_tag="VQ514_001509"
     CDS             8296..9276
                     /gene="dusB"
                     /locus_tag="VQ514_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HET2500254.1"
                     /translation="MTNLNTPFMIGNIEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA"
BASE COUNT         2729 a         2175 c         1914 g         2759 t
ORIGIN      
        1 atacgtctat ttatttttta ttgtttttta ttgtagagca gacccaagcg aatgaacata
       61 ggttgtaaaa agcaaatgaa aaaactgcga ggataatagc ttctgtgtct aaataaccca
      121 tttcgagacc tcttttcaac aacaacaagc ctgtagtttc gataacgatc aaacataaaa
      181 atatataaaa aattttcgca ttaagcatat tgttaccgct ttctttttat ttggttttat
      241 tgtataataa aagttgaaaa aaatcaaaaa tacaaaggag tttccgttat gtctaagatg
      301 tttaaatgca tgtgtttgct agttatgtct gttttctgta tgtctagcat ttccccgtcg
      361 cttgtttgtt tttgctgatg atagcttatc ggcaaactct aaagttgtat ctggtgaggc
      421 tcagtttgag aatggctcat cagtgcggtt cggagatacg caagtaaata ttttaagcga
      481 tgaagttctt gaggttgtca atccagatgg aagtgtagac actattgaac gacgtgctga
      541 cggcgtctat atcaatgggg cattttatat ggcttatcaa aagaatgaaa tcgacctaaa
      601 tatatctttt agatcatacg atcctaatgt ttggaattac gtcaacacaa tacatgggaa
      661 taaacaagct aacacattcg ctaacttcat gactggtgct ggaatcagtt atatgattgg
      721 taggataggt actttattgg ggggtccatg gggtgcaata attggtggag catattttgg
      781 tatacaagct tatcagtctt acttggattc acaaagccca tatccatact acataacaag
      841 tacttatatc catgttgctc aaaaaaaatg gaagtttatc actgaatact atagaaactc
      901 aaactatact gggtatgtaa aaaccgtaac aacctacgtg aatttctaaa atataaaccc
      961 tgcctagata taaaggcagg gtttatattt tagttaattg ctcaagtaat ttgtaagcat
     1021 aatacttgcg aaactctatt tgttcaagcg gttcaaaagc tcttgttttt tatcctcaag
     1081 ctcagctctc ctttgctcga tttcttcacg tttttgggcg atttctgctt gcgcctcggg
     1141 agtttccaaa gtgccttgtg acttgtgaaa ttctactagc atatcaacga tttttgggtc
     1201 taattcgttg acataatcag gatttgacca tttcggcacg ttgcttgcag gtgcttgctt
     1261 tttgccgcct tttttgggcg ttttggcttg ttgttcagct ttctgactgt agttgacctc
     1321 gtggaatttt cgtgcttgat atgatgcgtt ttattatgag aagatttact tcattttttg
     1381 ctgaaattgg gctcttgccc aagctctatc aacataattg ttttctaata ctcaatgaaa
     1441 atcaaagagc aaactaggaa gctagccgta agctgtactt gagtacggta aggcgacgct
     1501 gacgtggttt gaatttgatt ttcgaagagt ataaactaga aaagtgagaa tacaagcact
     1561 gccaaggctg ctccgataac agggcctaca acaggaatcc aagcgtaaga ccagtctccg
     1621 tctcccttgt ttggaattgg caagatgctg tgcatgatac gaggtccaag gtcacgagct
     1681 gggttcaagg cataacctgt tgtcccacct agtgatagac cgataccgac aatcaaagtt
     1741 cccactgcaa aggttccgat acctgcctga aaatcgtaaa gacccaaagc aaagattgtc
     1801 aacaccaaaa caaaggttcc aaggatttcg ctaatcaagt ttgatacagt atccttgatg
     1861 gctggtccag tactgaaggt tgccaggata ttgcctgcat tttcttctgc ctcatagtga
     1921 ggtttgaatt gcaaccaaac caaaatctga cccagcatgg cccctgcgaa ctgggctaag
     1981 atataaggca aaacggaagc ccaaggcaaa ccacctttta aggccacacc gatggtcaca
     2041 gctgggttta aatgagctgg actgagcttg ccagatacaa agactgcaac cgcaactgca
     2101 atcccccaac ccatagtaat cacaatccaa cctgagctat tgctcttggt tttaggaaga
     2161 accacacctg caacaacacc atttcctaga agaatcagga ttaaagtccc tagaaattct
     2221 ccaaataatt cattcatcat ctttctgtct ccattaaaaa gaagggacgg gcgacaagga
     2281 atgctgccct ccacctcttt tattttttct taatttttta attctgctaa atcgttgtta
     2341 gcgagagctg cttcgacatc agcacggtaa gttgcttttt cttcttctgt ccagtcatag
     2401 aatcgtccca tttcatccaa aattggctca acgatgctat ccaagctatc acgcataaag
     2461 agcatatggt tggtacgacg aagaaggaag tcaactgggc taagagccaa ctcgttgcgc
     2521 attgcatagt gaagggacaa agtatctgcc aagctgagtc ctggcgcttg ttccaagctg
     2581 tgagcaagtg caaagacttt cggtgcattt gaaccgtaaa gatttgccag atagtgagct
     2641 tccttgctat ccaaaccacg tgatactcca agttgcgcaa aggcttcgat ttctgaatcc
     2701 acatttgctg ggttcaattc tccacctgaa acagggtaag ttttagaatt gatcaattta
     2761 aagctacggt caaattctgc tttgaggatg tcaaccacgc gctccatagc tccttcagcc
     2821 atcttacggt agtctgtgat tttaccgcca gcaagagtca aaagaccatt gtcatcacgg
     2881 tccaagctag acccacgaga aactgcagat ggatccaaat gtttctcaga tgtgctactt
     2941 tcaagcttgc tgacagcaga ctcaacatct tcacgtgttt tttctttgga gagataagat
     3001 tcaacagtcg caatcaagtt gtcaaagctt tcatcactga tggtaccgtt atttccacca
     3061 ttatagtcag aggcactgtt ccctgcaatc aatggacgaa gacctgccca gctgctttcg
     3121 atatcatcaa tggtgatgtt ggattctgga aagcggttgt tgacaatgcc aagtagataa
     3181 tctacatctt cttgagttac ttttggatgc tccaaatcac ctgtgtagtc tgtatcagtt
     3241 gtaccaaagt aagtcttgtt ttcacgtggg agaacaaaga ccatacgacc gtcacccaaa
     3301 cctgtgtcaa agtaaactgg ctgtgaaacc ttgattttgc ttgaatccac taccaagtga
     3361 actcccttag ttgggcgcat ttgtgagaat tgcgttccct tattagacaa attacgtact
     3421 ttatcactcc aaggacctgt tgtattaata accagacggg ccttgatttc aaatacttgg
     3481 tctgtcaaga gatcacgagc tacaacacct gtaatcttgc cactttcgtc aaagaggaag
     3541 ccttctgcct tcacgtggtt ggcaatgagg gcaccgtctt ggttggcacg tttgatgttt
     3601 tcaatcacga gacgcgcatc gttgttacgg aagtcaagat acactccacc tcctaccaag
     3661 ccttccttct tcaagtttgg ctggcgttcc aagacttgat ccttgctcaa aaccttgttc
     3721 gcagttggtg tgttgctaac acctgccaag aggtcgtaca agtccatggc tactttaaga
     3781 cggaagaggc taaaggttgc tccatcttca tcgtaaactg gtaagagcat tgggtctggt
     3841 tttggaatgt gtggagcgat ttgttgaacc actgcacgtt cagaaaccgt atctgagacc
     3901 acttctacgt cgaattgttt aaggtaacga agtcctccgt gaaccaattt tgttgaacga
     3961 ctagatgttc cttctgcaaa gtcttgcatt tcaatcaaac cagtctcaag accgctagct
     4021 gccgcctgca aggctacacc agctcctgtg attcctccac cgataatcaa gaggtccagg
     4081 gtgcgttcct gcattttttt aattgacaat tcacgtgttt tctttgaaaa ttccatattt
     4141 acacactttc taaataaatc gctttattct gccagtatta gtcgtctact tccgcaaaga
     4201 cttgagttgc tttcacagcc ttcttccagc ccttgtagag ttgttccttg cgagattcgt
     4261 tcatagatgg ctcaaagagt tctcctgtct cgttcaagag tttcaactcg tccaagtctt
     4321 tccagtaccc tactgacaaa cctgctagga aggccgctcc tagagctgtt gtttccaggt
     4381 ttttagcacg tgcaatgtca atgcctaaaa tatccgcctg gaactgcatg aggaagttgt
     4441 tcatggctgc accaccatcc accttcagta cttgaatggc ggtctgagta tccacttgca
     4501 tggtgtcgat gatatcacgc acttgataag caatagattg caaagtcgcc ttgataaagt
     4561 cttctttgct tgttccacga gtcaaaccaa agacggaacc acgagcattt tggttccagt
     4621 atggagcgcc tagacctgta aaggctggaa cgacataaac ttcatcgttg ttgtgagaat
     4681 cacgagcgta tttttcagat tctggtgaat tttcaaccat gcgaagaccg tcacgaagcc
     4741 actgaatagc acttcctgcg atgaagatag aaccttccaa ggcataataa accttaccgt
     4801 tgattccgta accaatggtt gtcaagaggt tgttttcaga caactgcatc tcttccccag
     4861 tattcatgat gatgaaagag cctgttccat aagtattctt aaccatacct ggctcaaaag
     4921 ccaactgtcc aaagagggct gcttgttggt ccccagccat acctgagatt ggcacctctc
     4981 caccgtagaa atggaatgga gctgtcttgc cgtagatttc ggagttagaa cgaacttctg
     5041 gaagtatagc cttcggaatg ttaaggattt ccaaaatctc atcatcccat ttgagttctt
     5101 taatgttata aagcatggta cgagctgcat ttgagtagtc agtcacgtga gccgcaccgt
     5161 cagtcaattt ccaaaccaac caagtatcga tagtaccaaa gagcaattcc cctttttctg
     5221 ctcgctcttg agcaccttct acatgatcca aaatccaacg aaccttggta gcagagaagt
     5281 aagcatcaat aatcaaacca gtcttttcat ggaatttttc cacataacct tggcttttta
     5341 gttgctcagc caaaggtgct gtctggcgtg actgccaaac gatagcattg tagataggaa
     5401 gtcctgtttt cttatcccag acaaccgttg tttcacgttg gttggtaatc ccgattgcct
     5461 cgatttgatt tggcttgaca ccactttcga tgaaagcacc cgcaataact gactgaacag
     5521 agttccaaat ttcattggca ttgtgctcaa cccaacctgc ctgagggaaa atctgggtaa
     5581 actctttttg actcgagcta accttttccc cttttttgtt gaaaatgatg gcacgagaac
     5641 ttgtagttcc ctggtcaatg gccatgatgt atttttcttg tgacatgtac tgtcctcctg
     5701 ataaagatct tgaggtgttt cctctttaca cttactagta tacaaaataa agcgctttct
     5761 tgtttatcaa ctttttttaa tttttaaaaa aatgtgagga gattgtgtga atttcacaaa
     5821 aaagacctag attaaccaag ccttttaagt gaataataac tgacgaatcc ctgccaaatc
     5881 ttccatatcc aagtcgtttt ttaaataata gactggtgtc tgttccttct tatgaatcgg
     5941 gttattggta accagcaaat cataatgccg actagggtca taagcttcaa tggtaataaa
     6001 acgattgtat tccaaatacc gtttcaaaat ggctgccatc cttgaaaaga caagaaaacc
     6061 agatgtcaaa tccagaccaa tccgcatatg ctggccaccg ttttcagcca tatattcgat
     6121 taactggagc cattcccaga gaatcttctt atctaaatct gtcccctgtt gaaaagcagg
     6181 tagagcatga aaaatctcct ctgctgtctc tttaaaatca tgttccatca gtaaatatgg
     6241 atgacgattc tctaactggt acttgtagcg atcctgtaaa atatagccct tatagagata
     6301 gacttgagca caaagctgac tgagttcata ggtgacatgg tcctctgtat aatcccccaa
     6361 tagttcctcc ttcttcattt cttgaatcaa ttgcgtcagt aaatctgcca actgccctcc
     6421 aaaaccaaga atatactcca tagtatgaag aggaagaatg cgatgagata ggagaaaaga
     6481 gaagaatatc atcatctcac catcctcaac tcctagtgga atatgttgcc cagcaaaaaa
     6541 ggacggaacc tttctcagca aacgaagata aaagatattg tcaaaatacc ctcgcatttt
     6601 ctcttctatg acttgaaact gacaagcatt gacccgaaga cgttgttgac tgatgtgagc
     6661 ccagagaacc aagtccaatt tctgccccgc agacaaactt gcaccgcaga tttcctctaa
     6721 attggcaatc tcctgttttc tctctggttt ctgcatgtga ccttcccatt cctgactcga
     6781 ccagaccttt cggaaaagac agaaatagaa atagcgaatc tgatgctctg gacctctcca
     6841 gcgtccattt tggatggata aatcaaattc tgacaaaatc tgatttaaac cagccaagtg
     6901 acgaccaagc gtagcctcgc taatcaccaa ttcttgagcc agctgatggg ctaaaaactg
     6961 ttggtggtag agaagataaa ccaaaatctg gtatttaaca gcactatcca aaaacaagct
     7021 ccgaatatct ctccccttgg tagctgcacc gatagacaga cgcagatttt catcttctga
     7081 gtgaatagtc agttctaagc cactatccaa agccttgtca ttgagcaggg tgacatattt
     7141 ggttagggtt gcttttgaaa atcctgtttc ttccattaca gccttgacgg tcgtctgaga
     7201 ctcttgtaat agaaaggaaa ggattgaaaa ttgaccacac tcggctttct ccatcaaatc
     7261 tcctaaatac atttgttacc cctttcattt tctaccttaa gcatagcata aatcttacaa
     7321 atgctgaaat aatctgtttc tctttttatt ttcatgctga tttcctggtc cattatcctg
     7381 aaaatcagta aacacacggc tccccctttg ggcattttta tgctaaaata gtagctatgg
     7441 ataaaattat taaaactata tcagaaagcg gagcctttcg tgcttttgtc cttgatagca
     7501 cggaaaccgt ccgcactgct caagaaagac atcaaaccca agctagctca actgtagcgc
     7561 ttggtcgaac tcttatcgct agccagattc tcgcagccaa tgaaaaagga aataccaaac
     7621 ttacagttaa ggtgttgggc tctagctctc taggtgctat tatcaccgtc gctgatacca
     7681 aggggaacgt caaaggctat gttcaaaatc ctggtgttga catcaaaaag actgcgactg
     7741 gtgaagtcct agtcggacct tttgttggaa atggtcaatt cctcgttatc acagactacg
     7801 gtgctggaaa tccttacaac tctataactc ccctcatctc tggagaaatc ggtgaagacc
     7861 ttgcctttta ccttactgaa agccaacaaa cgccttcagc ggtcggcctc aatgtccttt
     7921 tggacgagga agacaaggtc aaggttgcag gtggtttcct agttcaagtc ttgccaggag
     7981 ccaagaaaga agagattgct cgctttgaaa aacgcatcca agaaatgcca gctatctcta
     8041 ctcttctcga aagcgacgac catatcgaag ccctcctcaa ggctatctac ggggacgaag
     8101 cctacaagcg tctttctgaa gaagaaatcc gtttccaatg tgactgtagc catgaacgct
     8161 ttatgaacgc tcttgccagc cttccaagct cagacttaca ggaaatgaaa gaggaagacc
     8221 acggggcaga aatcacttgt caattctgcc aaactactta caactttgat gaaaaggacc
     8281 tggaggaact cattcgtgac aaatcttaat acacctttta tgattggcaa tattgagatt
     8341 cccaatcgta ccgttttagc gcctatggct ggcgtgacca actcagcctt tcgtaccatc
     8401 gcaaaagagc tcggagctgg actcgttgta atggaaatgg tctctgacaa gggaatccaa
     8461 tacaacaacg aaaaaaccct gcacatgctt catatcgatg agggcgaaaa ccctgtctct
     8521 atccaacttt ttggtagcga tgaagacagc ctagcacgcg cagcagaatt catccaagaa
     8581 aacaccaaga ccgatatcgt cgatatcaac atgggctgcc ctgtcaacaa aatcgtgaag
     8641 aacgaagctg gagctatgtg gctcaaggat cctgacaaga tctactctat catcaacaag
     8701 gtccagtctg tccttgatat cccacttact gtcaaaatgc gtaccggatg ggcggaccca
     8761 tctcttgcag tagaaaatgc cctcgctgct gaggctgcag gtgtttctgc cctcgccatg
     8821 catggccgta cccgtgaaca aatgtatact ggccacgcag accttgagac cctttacaag
     8881 gttgcccaag ctctaaccaa gattccattc atcgccaacg gtgatatccg tactgtccaa
     8941 gaagccaagc aacgcatcga agaagttggt gctgacgcag tcatgattgg ccgagctgcc
     9001 atgggaaatc cttacctctt caaccaaatc aaccattact ttgaaacagg agaaatccta
     9061 cctgatttga cctttgaaga caagatgaag atcgcctacg aacacttgaa acgattgatt
     9121 aacctcaaag gagaaaacgt cgcagttcgt gaattccgcg gcctcgctcc tcactatctc
     9181 cgtggaacat ctggcgctgc caaactccgt ggagccattt cgcaagctag caccctggca
     9241 gagattgaag ccctcttgca attggagaag gcttaatagt ttaaaacccg taactctctt
     9301 aaagagcctc ttgaatgccg ccattctttc ctcatcaaaa aaactccgta gtctctagcg
     9361 ctataaatcc ggctagagat tacagagtta tttttttatc taaacttcct acaagggtat
     9421 attatctact gaaaggatat ggtatttgta gggaatactt taaagagttc ttacaggaag
     9481 tcagtattaa ctagttatat taggtttagt ttacccattc accattggca ttgactctat
     9541 agctatctac agttgtgttg actgcaaggg cacctga
//
LOCUS       DAVBCW010000075         9431 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVBCW010000075 DAVBCW010000000
VERSION     DAVBCW010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9431
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.075"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(97..786)
                     /gene="rplA"
                     /locus_tag="VQ514_001510"
     CDS             complement(97..786)
                     /gene="rplA"
                     /locus_tag="VQ514_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HET2500255.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMGLVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            complement(993..1418)
                     /gene="rplK"
                     /locus_tag="VQ514_001511"
     CDS             complement(993..1418)
                     /gene="rplK"
                     /locus_tag="VQ514_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HET2500256.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAVRMIEGTARSMGFTVID"
     gene            complement(1541..2257)
                     /locus_tag="VQ514_001512"
     CDS             complement(1541..2257)
                     /locus_tag="VQ514_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HET2500257.1"
                     /translation="MKKRYLVLTALLALSLAACSQEKAKNEDGAAKTEQTAKADGTVG
                     SKSQGAAQKKAEVVNKGDYYSIQGKYDEIIVANKHYPLSKDYNPGENPTAKAELVKLI
                     KAMQEAGFPISDHYSGFRSYETQTKLYQDYVNQDGKEAADRYSARPGYSEHQTGLAFD
                     VIGTNGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYFGFEGGDYVD"
     gene            complement(2260..2730)
                     /locus_tag="VQ514_001513"
     CDS             complement(2260..2730)
                     /locus_tag="VQ514_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HET2500258.1"
                     /translation="MCLICQRIELIKKGENPYFVKELETGYLVIGDHQYFAGYSLFLA
                     KEHVSELHHLEKKTRIRFLEEMSVVQEAVAKAFAAEKMNIELLGNGDAHLHWHLFPRR
                     RGDMNGYGLNGRGPVWWIPFEEMTAEICQSKPDEIKRLVERLSSEVDKLLEIKE"
     gene            complement(2732..3769)
                     /locus_tag="VQ514_001514"
     CDS             complement(2732..3769)
                     /locus_tag="VQ514_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HET2500259.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQKVLGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDPRTVVTDTGFIKSRHLDDKVSAAMLLNLLRIYKKEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTNEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVVATERM
                     VDAYLKSALVD"
     gene            complement(3867..5192)
                     /gene="brnQ"
                     /locus_tag="VQ514_001515"
     CDS             complement(3867..5192)
                     /gene="brnQ"
                     /locus_tag="VQ514_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HET2500260.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            complement(5363..5455)
                     /locus_tag="VQ514_001516"
     CDS             complement(5363..5455)
                     /locus_tag="VQ514_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET2500261.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            complement(5498..5650)
                     /locus_tag="VQ514_001517"
     CDS             complement(5498..5650)
                     /locus_tag="VQ514_001517"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET2500262.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLEGAHNLPLSQ
                     LADSYD"
     gene            complement(5810..6388)
                     /locus_tag="VQ514_001518"
     CDS             complement(5810..6388)
                     /locus_tag="VQ514_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HET2500263.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPCT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            complement(6442..7842)
                     /gene="pepV"
                     /locus_tag="VQ514_001519"
     CDS             complement(6442..7842)
                     /gene="pepV"
                     /locus_tag="VQ514_001519"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HET2500264.1"
                     /translation="MIAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGAARLHSFTGGLREN
                     MVPESATAVVSGDLTDLQAKLDAFVAEHKLRGELQEEAGQYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVASVSLSEHGHTPHYVPMEDPLV
                     QTLLNVYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            complement(7855..8460)
                     /locus_tag="VQ514_001520"
     CDS             complement(7855..8460)
                     /locus_tag="VQ514_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET2500265.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDLKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDDKLEPSYRLPVDEIIEKR"
     gene            8631..9431
                     /locus_tag="VQ514_001521"
     CDS             8631..9431
                     /locus_tag="VQ514_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2500266.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
BASE COUNT         2665 a         2101 c         1841 g         2824 t
ORIGIN      
        1 gaggacttac acctcagata tacgacatgc ccgtcgtaca tcaaaaaaag ttgagacata
       61 gcgtcttcaa cttttttaca ttactttaac tactgattaa agtgagttta cgtcaacttt
      121 gataccaaca ccttgagtag ttgtgatagt caagtttgtt acgtaagttc ctttagctgt
      181 agctggtttt gctttttgga ttgtttcgtt gaaagcttta aagttttcaa ccaatttttc
      241 agcttcaaat gatactttac cgatgattgc ttgaacgtta cctgcacggt cagcacggta
      301 agtgatttta ccacctttag actcttcaac tgctttagca acatccattg ttacagtacc
      361 agttttaggg tttggcatca agttacgtgg tccaaggaca cgtccaagac gtccaacaag
      421 acccatcata tcaggtgtag cgataactac gtcgaagtcc aaccaaccgt cgttgatttt
      481 agcaacaagg tcatcttcac caacaaagtc tgcaccagca gcttttgctt cttcagcttt
      541 tgcaccacgt gcgaaaacaa gaacgcgtga agttttacca gtaccgtttg gcaataccat
      601 tgctccacgg atttgttgat cagctttttt aacgtcgatg ttcaagttgt aagcaacttc
      661 tacagttgcg tcaaattttg caaagtttgt ttcttttgca agtgctacag cttcttctac
      721 gctgtatgct tttgtgctgt cgattttctc aagagcagca cgaagttgtt tgcttttttt
      781 agccattttc tattctcctt gtaagtggtt caatcgattt tcatctccca cgtcacttct
      841 cgaaatgatg aagtcttgcg ggttttcagt ctattagatt cttttcctac ggccttaagc
      901 tcccttgctg tacccaagta caagcatcag tcacttgcct agtatgaaaa gcaactaata
      961 gactgctgtg agattttcta ctgtgttatt gattagtcaa taacagtgaa tcccatagaa
     1021 cgagcagtac cttcgatcat acgcactgca gactctacgt ttgctgcgtt caaatctggc
     1081 atcttagttt ctgcaatttc ttgtacttgt gcacgagtaa ctgtagcaac tttagtttta
     1141 ttaggtgtac ctgatccttt ttcaacacct gcagcttttt tcaaaagaac agcagctggt
     1201 ggtgttttcg taacgaaagt aaatgattta tcttcgtaaa ctgagataac aactggaatg
     1261 atcataccag cttggtcagc tgtacgagcg ttgaactctt ttgtgaatcc catgatgttg
     1321 ataccagctt gaccaagagc aggtccaacc ggtggagctg gtgtagcttt accagcaggg
     1381 atttgcaatt ttacaagttt ttcgactttt ttagccattt ttaaatcctc ctttgtggtt
     1441 ttggcggtaa ttagagattt ttacctccca caagtatgtt tttcacatac ccatctatta
     1501 tacactattt atctggaatt gcaagagaaa agtttatttt ttaatcgacg taatctccac
     1561 cttcaaagcc gaaatactct tccaaactga ggccactagc tgcaatctct ttagcctctt
     1621 ttccgacata acgaaggtgc cattcctcag ccatatagcc tgtttccttt tccttacctt
     1681 tgagataacg tacaacaaag ccataatcag ccgcatgatc caagagccat tgggccgctt
     1741 tttcttctgt caccaaatca ccatttgtcc caatcacatc aaaggctaaa cctgtctggt
     1801 gctcgctata gccaggacgg gcagaataac ggtcggcagc ttctttccca tcttgattga
     1861 cataatcttg atagagctta gtctgagttt cataacttct aaagccactg taatgatcgc
     1921 taatggggaa acctgcctct tgcatagctt tgatgagctt gaccaactca gccttggctg
     1981 ttggattttc ccctggattg tagtcttttg acaatggata gtgtttgttg gctacgatga
     2041 tttcatcgta tttcccttga atgctgtaat agtctccttt gttaaccact tctgcttttt
     2101 tctgggcagc tccttgagac ttacttccta ctgttccatc agctttagca gtttgctctg
     2161 tcttagctgc tccatcttca ttttttgctt tttcttgtga acaagctgct agactcaagg
     2221 ctagcaaggc tgtcaagaca aggtatcttt ttttcatctt tactccttta tttctaatag
     2281 tttatctact tctgatgata aacgttcgac taatctttta atctcatcag gttttgattg
     2341 gcagatttct gctgtcattt cttcaaaggg aatccaccat actggaccac gtccattgag
     2401 gccataacca ttcatatcac ctcttcgtct aggaaacaga tgccaatgga gatgagcatc
     2461 gccatttcct agtagttcga tattcatttt ctcagcagca aaggccttgg caactgcctc
     2521 ttggaccaca ctcatttctt ctagaaaacg tattctagtt ttcttttcta aatgatgcaa
     2581 ttcgctgaca tgttccttgg ctagaaagag actatagcct gcaaaatact ggtggtctcc
     2641 aatcacaaga tagcctgttt ccaactcttt gacaaaatag ggattttctc ccttcttgat
     2701 gagctcaatt ctctggcaaa tcaaacacat attagtctac caatgcactc ttaagatagg
     2761 catcgaccat tcgttctgtt gcgaccaccg agtcaatatg agtgcgctca taagaatgac
     2821 tagactcgat acccgcacca agaagggcat gcttgacctc tgcccctgca gacatagccg
     2881 ctgaagcatc cgaaccataa aatggataga tgtccagctt aaaaggaata tcttgctctt
     2941 tagccaaggc aaccaagtgt tgacggaagt catagtgata aggacctgag gcatccttga
     3001 cacagataga tactgtgtac tcatttgtct gctggtcatc tcccatagct cccatatcca
     3061 cagccagata ttctactacc tgagcaggaa tgttagaatt agcaccatgt cctacttctt
     3121 caaaaactga aaaagcaaaa tgagttgtta ctggcaattc aatcttctct ttcttataaa
     3181 tacgaagcag gttgagcaac atcgctgcgc tgaccttatc atccaaatga cgagacttga
     3241 taaaacctgt atcagtcaca acagttcgtg gatcgaagct gataaaatca ccgacctcaa
     3301 tcccaagagc acgagtttct ttttcattgc ttacttttac atccaaacgc acttccatat
     3361 tgtcctgcgt gcgttctgca gttcctgcat ccttgtagac atggcaagaa gtttggtgga
     3421 tgaggatggt tcctaatacc ttttgacctg tgctagccac atgaacggta cagttttcac
     3481 cttcaatcat gttccaagga aaaccaccga tacggtccaa tttaagacga ccatctggtt
     3541 tgacagcacg aacaatagca cccagtgtgt ccacatgggc agtcacatag cgatgttgct
     3601 catcattttg acctttgatg gtcacattga caccaccctt ggctgtgcga actggctggt
     3661 aaccaaaacc ttctagagtc ttgactaaat aattcgaaat ttcacgagtg aagcccgttg
     3721 gcgatgcgat ggctgtcagt tctttgatat attctactgt ttgattcatt tcttcacctc
     3781 tttcgctacc tattataaca catttatcat cggataaaaa aagaaaatca ctcctgtaga
     3841 cttggctaca aaagtgattt aagtgattat tccatttcaa aaacatcgct ttcttgcttg
     3901 tttggtagaa ccaatgagag caagattcca acaatggctg gcaccaacca tgggagagat
     3961 gccttggcaa aaggaagaac gctaacaaga tttgcaagaa actcaacctt aaacgagctt
     4021 cctagtacgc ttgcaatggc aataactgta accacagcaa ttgtcaactg catacctggt
     4081 tttgaaaggg caacaaattt gttgacaatg acaatcataa cgatagcaat cgtgattggg
     4141 tacaagataa ccagtactgg aattgagtac ttgataatcg catcaagacc caaattggca
     4201 atagcaaatc caatcaaggt aaaggctgtc gcataaacct tgtagctgat ttgtgggaag
     4261 cgctcattaa agaactcagc tgttgacaca atcaaaccaa cagtcgttgt gaagcaggtt
     4321 acggtaacca tagctgcaag gaagagttga gctgttgagc caaagatttc ttgagtggct
     4381 tgtgacaaga tgtaaacacc tggtgttcca cccttcatcg cttcagctgg tactgggaaa
     4441 tgatttccaa gaaaacctaa accgatgtaa agagcgctga aggcaagggc aacaacgata
     4501 ccaacaaccc aaatagttga aatgtattct ttcttacttg aaaatccaag ttgtttcaag
     4561 gtttgaactg cgattacgct aaaggccact gaggcaaggg cgtccaaggt attataacct
     4621 tctaggaaac ctgtaccaaa ggcagaagct tgataagcag ctgaagcagc ttgaggactt
     4681 gttccaccat atttgatagc tcccagaacg accaagataa caatcaaaat tgcaaagact
     4741 ggcgttaaaa tacgtccaat gcggtctaag atttttgatg gattaagcga aatcaaatag
     4801 gctgccgcaa aatacagaac cgtaaataca atcaagccaa gtcctttatt tgcatccgac
     4861 aaaagggggc taatccctac ttcgtaagct gttgtagcag tacgtggggt agcaaagaat
     4921 ggaccgattg acaagtaaag aactgagagg taaagagtcg caaaccaagg cgctatcttc
     4981 gttgaaatct cgtagatata tcctttagga tttagcgttc caataataag ggtcaagacg
     5041 gcgataccaa cgcctgaaaa gacaaaacct gcgatggcag gaagaaaatg ttctccagat
     5101 agagcaccta gagaaggcgg aaaaatcaag ttccccgcac caaaaaatat tccaaacagg
     5161 agcaaacctg ttagggtacc ttttttagcc ataaaaatct ccttcatatt tataaaatac
     5221 tgatctagta taccacgatt aggagtctga aaaaagcatc acgaggcaaa tattaaatgt
     5281 tttcaatttt cttaaaaata agaattatct aaaaataaaa ctctcctacc cagtccacac
     5341 caagtaggag aaatgcaaaa ttttaaagtt cttcaaaggc taacatgcca ccctggacat
     5401 tgataacatt ataaccttgt tctaatagga attggcaagc acgcgccgat ctcattccag
     5461 atttgcaaat aatataatgc aagcgatctt tgtccaatta atcataacta tcagccaatt
     5521 gactaagcgg taggttgtgg gcaccttcta aatgaagagc tgcaaactca tccacttctc
     5581 tcacatccac tagagaaagc tggtcgtttt ggtaaagctg gtaaaatgcg tcaaaggcta
     5641 tttctttcat tctttttctt ctaatactct tcgaaaatct cttcaaacca cgtcagcgtc
     5701 gccttaccgt gcgtatgtta ccgacttcgt cagttttatc tgcaacttca aagctgtact
     5761 ttgagcagcc tacgactagc ttcctagttt gctctttgat tttcattgac tataaaatgg
     5821 ttttaattct ttttttcaaa tctggcacta cttctgcctc aaaccaagga tttttggcca
     5881 tccagatttg atttcgtggt gatgggtgaa ctagcggaaa ataggctggc agatagtctt
     5941 tatagtgttt caccctctcc gttaccttcc cactgatttt ctcctgtaaa tagtaggctt
     6001 gggcatattg cccaatcaag agggttaact gaatatcagg caattcctgt aagagctgcg
     6061 gatgccattt ttctgcaaaa cctgtacaag gcggaagatc acccgacttg ccatgtcctg
     6121 gaaagtagaa atccataggc aaaacagcaa aataacctga attgtaaaag gtatcttcat
     6181 ccacacctag ccagtcccgc aaacggtcac cacttttatc tttccagtaa aggcctgctt
     6241 cttgagtttt aagtcccgga gcctgaccga tgatattgat gcgagcagtt tttggcgctg
     6301 caaagagagg ctcaatgcca cgctctgtat agctggcatt ctgcgaatcc gccatgatag
     6361 cctgtttgat tctttcgatt tgagacatct actttccctc caaaaagaag tccaagcata
     6421 agatctcaga cttctatcgt tttatttgat caattcgtaa atagcttcgg cataaattgc
     6481 tgctgctcgg aagagatcgt ccaaggcaat aaattcattg gcttggtgca tggtatcaat
     6541 tgagtctggg aacatagcac cataggcaac tccgcgctct aacaagcgac caaaggttcc
     6601 accaccgatg acttgttcat gacctttaag gcctgtttgt ttttcataga cattcaacaa
     6661 ggtttgcaca agtggatctt ccattggcac atagtgaggg gtatgaccat gttctgatag
     6721 actgacagaa gcaactggca agttttctag gattgacttg atttgttctg gacttgttcc
     6781 ttttggatag cggatgttaa gggcaatggt attatcagca cttgtttcat cgaagcggaa
     6841 gacgccggca ttcatagaaa gagcacccat cttttcatcc acatgagcaa tcttgagatt
     6901 ttcaccctca tgatcgttca agagaatttt acctgcgatg tcaaggtagt ctttggctgg
     6961 accagcaaag ccaaactggc tgaggaagag ggcaaggtaa gttgcgccat tgacacctga
     7021 agcaggcata gcaccgtggg ctgatttacc aatgatcgtc accttgtatt ggccagcttc
     7081 ttcttggagt tctcctctaa gtttgtgttc tgcaacaaag gcatctagtt tagcttgcaa
     7141 gtctgtcaag tcacctgaaa cgactgctgt tgctgattct ggtaccatat tttcacgtaa
     7201 accacctgta aagctgtgaa gacgggctgc acctgtattt tcacctgcaa agtggaggta
     7261 ttccgtgatg tttccttttt caccattgat gattggaaat tcagcatctg gtgagaaacc
     7321 aaagtctggc ttggcgagtc ctacgtgctc gaagtagtag tccatatctg cccagcctga
     7381 ttcttcgtct gttccaacga tgaagcgaac tttcttagaa gttggaaggc ccaattcttt
     7441 gatgattttc aaaccatagt agcaagctgt tgtaggtccc ttgtcatctg aagcaccacg
     7501 cgcataaagg cgaccatctt tgatagttgg tgtgtaaggg tctgtgtccc aaccgctacc
     7561 agctggaacc acgtccatat gggcaaagat tccgagaact tcttctccat caccaaactc
     7621 aaaatgtcct gcatagttat caacattttt agttggataa ccatcacggt ctgcgatttc
     7681 aaggaatttt tccaaggctt ttactggacc aggtccaaat ggatgttggg catcagcctt
     7741 gctatcatca cgttctgagt tgatttccaa aaggctaaac aagtcagcca agaggtcttc
     7801 tttgcgtttt tctacttctg ctgtaaaatc aattgctatc attttttctt cttcctatct
     7861 tttttcgatg atttcatcta ctggcaagcg gtagcttggt tccaatttgt cgtctgtgta
     7921 acccactgtg atcaagagtt ctgggcggaa acggtcttca atttccaaaa cctcattgac
     7981 ttttgatttg tcaaaaccaa ggataatatt ggaaccaatt ccttggtctg taagagcgag
     8041 aaccaagttc atagcaacca aacctgcatt gagtgctagg tagtcgctga cttgttgttc
     8101 attgtagcgt gcaaattcag ctggcaaatt tttcatgaag tattgaagtt gttcttctga
     8161 gaaattgtta gcaccaccaa ctcgggcaat cttacgagcg cgtttggcaa ggtctgtatc
     8221 tgtaaatagg gcaatggtta caggtgctga tgatacctgc tcaaagtttg aaccataagc
     8281 caactttgcc agttcagcat ttttctcacg aacaactaca aatttccaag gctggctgtt
     8341 gtgggcgctt ggcgccaagg ttgcaatttc gatagccgta cgcacatctt ttagatcaac
     8401 aggcttatca gtgaaatgct tggtcgcatg acgtttttta tttaattcaa gaaatttcat
     8461 aatcgatttc cttttctaat tctactgctt ccattctacc ataatttgaa agagcttgca
     8521 gggatttttc atgattaagt ttttctatca cagacataaa agatatatat tggtttatca
     8581 ggaaagtttc tgataaacta gcaagtactt tacatttgaa tggagacagt atgaaaaaat
     8641 ttagcctatt actagctatc ctaccatttt tggttgcctg tgggaatcaa gctacaccca
     8701 aagagactag cgctcaaaag acaatcgtcc ttgctacagc tggcgacgtg ccaccatttg
     8761 actacgaaga taaaggcaat ctgacaggct tcgatatcga agttctaaag gcagtagatg
     8821 aaaaactcag cgactacgag attcaatttc aaagaaccgc ctgggaaagt atcttcccag
     8881 gacttgattc tggtcactat caggctgcgg ccaataattt gagttacaca aaagagcgtg
     8941 ctgaaaaata cctttactcg cttccgattt ccaacaatcc cctcgtcctt gtcagcaaca
     9001 agaaaaatcc tttgacttct cttgaccaga tcgctggtaa aacaacacaa gaggataccg
     9061 gaacttctaa cgctcaattc atcaataact ggaatcagaa acacactgat aatcccgcta
     9121 caattaattt ttctggtgag gatattggta aacgaatcct agaccttgct aacggagagt
     9181 ttgatttcct agtttttgac aaggtatccg ttcaaaagat tatcaaggac cgtggtttag
     9241 acctctcagt cgttgattta ccttctgcag atagccccag caattatatc attttctcaa
     9301 gcgaccaaaa agagtttaaa gagcaatttg ataaagcact caaagaactc tatcaagacg
     9361 gaacccttga aaaactcagc aatacctatc taggtggttc ttacctccca gatcaatctc
     9421 agttacaata a
//
LOCUS       DAVBCW010000076         9429 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVBCW010000076 DAVBCW010000000
VERSION     DAVBCW010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9429
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.076"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            143..676
                     /gene="queF"
                     /locus_tag="VQ514_001522"
     CDS             143..676
                     /gene="queF"
                     /locus_tag="VQ514_001522"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HET2500267.1"
                     /translation="MKHQSALVVFSGGQDSTTCLFWTMQHYETVEAVTFAYGQHHHLE
                     IQITREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
     gene            complement(930..1598)
                     /locus_tag="VQ514_001523"
     CDS             complement(930..1598)
                     /locus_tag="VQ514_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HET2500268.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGLDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLSN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
     gene            complement(1709..2263)
                     /locus_tag="VQ514_001524"
     CDS             complement(1709..2263)
                     /locus_tag="VQ514_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HET2500269.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLFDHLGNPSPEIRNVLLNQGL
                     HYLSKEKDTRGFSSQYGWVHAFAHGADLLTEVVCHPGFPKNRVHEVFEILGQLFKRMS
                     IRFTDDEDWRLARVIYEPILQGKLAQEQVASWIKTVDFPIEEREDFYKFSNFRSCLVE
                     VYVQLDQRNSLQDELKEAIQSFQY"
     gene            complement(2509..4305)
                     /gene="pepF"
                     /locus_tag="VQ514_001525"
     CDS             complement(2509..4305)
                     /gene="pepF"
                     /locus_tag="VQ514_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HET2500270.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYGFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVAADEEVLDRLGKLPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDATLTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKHSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIISYSAELGE"
     gene            complement(4316..5059)
                     /locus_tag="VQ514_001526"
     CDS             complement(4316..5059)
                     /locus_tag="VQ514_001526"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HET2500271.1"
                     /translation="MQQYFVKGSAVSPVTIEDRETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFITQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNVVPSIHLFENKADFLAQLDQ
                     FDRILVAYEEAAKEGEAAALIKAVTGLEKGAKLLFIFGPEGGLSPAEIENFEAKGAVL
                     AGLGPRILRAETAPLYALSALSVLVELEK"
     gene            complement(5061..6011)
                     /locus_tag="VQ514_001527"
     CDS             complement(5061..6011)
                     /locus_tag="VQ514_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HET2500272.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYMGNVD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDMTVVEADLQARLAELTDFMDLGELKIGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNTG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLTEDAYRLVKDEGYLIMSGIIKDKW
                     DMVRQSAESAGFFLETHMIQGEWNACVFKKTKDISGVIGG"
     gene            complement(6149..6577)
                     /locus_tag="VQ514_001528"
     CDS             complement(6149..6577)
                     /locus_tag="VQ514_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2500273.1"
                     /translation="MELEISDFQGCKIALFCGDKLLTILRDDKDDIPCPNMWELPGGG
                     REGDESPFECARREVYEELGIHLDEDCLLWSKIYPSVIFKGKKSVFMVGQLRQEQFDN
                     IIFGDEGQGYQLMNVEEFLSSSQVVPQLQERLKDYLKVSD"
     gene            complement(6558..7646)
                     /locus_tag="VQ514_001529"
     CDS             complement(6558..7646)
                     /locus_tag="VQ514_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET2500274.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQLKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNF"
     gene            complement(7665..8135)
                     /locus_tag="VQ514_001530"
     CDS             complement(7665..8135)
                     /locus_tag="VQ514_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HET2500275.1"
                     /translation="MATYGFLDVLEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNEEDYLTVIPYLPKKGFSREFLAYF
                     VLFLKDTAEVGLDVLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            complement(8426..8878)
                     /locus_tag="VQ514_001531"
     CDS             complement(8426..8878)
                     /locus_tag="VQ514_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HET2500276.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            complement(8915..9094)
                     /locus_tag="VQ514_001532"
     CDS             complement(8915..9094)
                     /locus_tag="VQ514_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HET2500277.1"
                     /translation="MPMTQKEMVKLFTAHGWIKTRGGKGSHIKIEKQGERPITILHGE
                     LNKYTERGIGKQAGL"
     gene            complement(9231..9410)
                     /locus_tag="VQ514_001533"
     CDS             complement(9231..9410)
                     /locus_tag="VQ514_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500278.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSGPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
BASE COUNT         2785 a         2260 c         1694 g         2690 t
ORIGIN      
        1 ttatttcatt ttactatata agtagaatat tacgatgttt gggatatgcc atcgctaaaa
       61 aaaaactcga tttggtattt tttagttccc taatgggagc tttttgcgtg ctctgaacat
      121 ttcccatttt ggaaggagta ctatgaaaca tcaatcagcc ttggtcgtct ttagtggcgg
      181 tcaagattca acaacctgcc ttttctggac catgcaacac tatgaaacag tcgaagctgt
      241 cacctttgcc tacggccaac atcatcacct cgaaattcaa attactagag aaatcgctaa
      301 ggaacagggc attcgtcacc atatcctcga tatgtctctg ctgggacaaa tcactgctca
      361 gccagacttt gcgacgattc atatttccta cattcctgac aagctctgtg tcgagtcaaa
      421 atccctcaaa ctatatctat ttagctaccg aaaccacgga gatttccacg aaaactgtat
      481 caacaccatc gggaaagact tggtcaactt gctagaccct cgctatttag aagtctgggg
      541 aaaattcact ccgcgcggtg gcatttcaat cgacccctac tataactacg gtaagcaagg
      601 aactaagtat gagggcttgg cagaacaacg cctcttccaa cacgaccttt atccagagaa
      661 aattgacaac cgctaaagtc atactcaatg aaaatcaaag agcaaactag gaagttagcc
      721 gcaggttact caaagcagtg tttgaggttg tagataaaac tgacgaagtc agatcaaaac
      781 actgttttga ggttgtggat agaactgacg tagtcagtaa ccatacctac ggcaaggtaa
      841 agctgacgtg gtttgaagag attttcgaag agtatcatac gaaaaagccc tgttcctcaa
      901 gatgaggagc aaggcttttt gagtttcaat tattcttctg ttccaaggaa attttttgca
      961 acaagggctg caagaactcc accagcgatt ggtgcaagga tgaaaatcca aacttgttga
     1021 agggctgcgc cgcctaccaa gacagctggt gccaagctac gagctgggtt tactgaaagt
     1081 ccagtaatct tcaatccgac aagaatcatc gccatcaatg acaaaccgat taccaaacca
     1141 gcaatcgcgc cattgccctt gctttctgaa gtcacagtca tgataaccaa tacaaacaag
     1201 aaagttgcga tgacttcaaa caagaaacca ccaaagacag tgacaccgtt tgccaaggca
     1261 ttttcaccaa gactagcagt tgacatacct gaattactca agaggaagaa gactgcgcca
     1321 gaagcgatga aagctccaac aacctgacca aggatgtagt ttacaagttc tgaagatgac
     1381 aaacgtttgt ttacaaacat agcaatcgaa acagccgggt tcaagtgagc acctgaaaca
     1441 gttccgattg agtaggctgc caccacgatt gccaaaccaa aggcaaaggc gattccaagg
     1501 tgaccaaggc catcaagacc atttccaaaa acaacagctc ctgttccaac gaacacaagc
     1561 atgaacgtac cgattaactc agcaacaaat tttttcattt tctttctcct tttttcaaaa
     1621 actagatact agtctatcaa aagtaggaaa gggtttcaag aaaattgatt ggaaattttt
     1681 tgaaaatcat agaactatta gctaatctct agtattgaaa agactggata gcttctttca
     1741 actcatcttg taaactattt ctctggtcaa gttggacata gacttccacc agacaggatc
     1801 taaagttgga aaatttgtaa aaatcctccc tttcttctat cggaaaatca acagttttta
     1861 tccaagaagc tacttgttct tgcgctaact tcccttgtaa aataggttca tagatcactc
     1921 ttgctaaacg ccaatcctca tcatctgtaa agcgaatcga cattctttta aataattggc
     1981 caagtatctc aaatacttca tgaactctgt ttttaggaaa gcccggatga caaaccacct
     2041 ctgtcagtaa atcggctcca tgtgcaaaag catgaaccca accatactga cttgaaaaac
     2101 cccttgtatc cttttctttt gaaagatagt gcaagccttg atttaaaagg acattacgaa
     2161 tttctggaga aggatttccc aaatgatcaa acaaccactg gatttcttcc tggttataat
     2221 ttggtttttc ttctgctatt tttcttagta aatcttgata catggtcaat acctctacat
     2281 ttctagcaac tgttcaaaaa ggcagtctta aatgactcaa tattgaattc tcaattaaat
     2341 acaatctgat ataaaatgac gtaaataact atcaatacca gttctacagt aagttcaaat
     2401 ttaacatcac gatcttcaac gacatttttg aaaatagcta caactaagac aaatagaatg
     2461 acgcttaaca agcccataaa catcattcta aaaaattttt ctattcccct actctcccaa
     2521 ctcagcacta taggagataa tctggtcaac tgtgtcagac aagaattgga tggtatcacg
     2581 gagtggtttg tctgttgaaa tatcagcacc gataatcatg gctgactcaa gtggtgtctt
     2641 gctaccacct gatttgagga gattgagcca gtcttcagct ccagtttcag aatgttttag
     2701 atgaaggtaa ccagcagtcg agataactag tcctgctgag taagtgtaac tatacaagcc
     2761 catatagtag tgagcttggc gcatccaagt cagagttgca tcatcgtcaa tttcaatagc
     2821 atctccccag aaatccgtca aaacttcctt cataatgctg ttgagcttgc ttgctccaaa
     2881 ggtctcccct tcttcaatca atgtatacac cttacgctgg aaggcggctt ccaagaggtg
     2941 ggtgataaag ttatggaagt aggtgtctgt caagcgatga gccagagcga agcgtttttg
     3001 acgtgggtca ttagactggt tctccaagta atcactgagt agcaattcat tgaaggttga
     3061 cggtgcttca acatagtagg tcgacatatg ggcattgaag taactttgat gattgtctga
     3121 aaagatgaat tgaccagaat gcccgatttc atgaatcaag gtatagacat cgctcaaacg
     3181 gcctgtccag ctcatgagta cataagggtg tacgcgatat gggtccgccg cataaccacc
     3241 ggaatccttg ccactgttag cagcaaagtc cacccagcgc tcttcttggt aacgagcaac
     3301 ttcctgacaa tattcttgcc ccaaaggttc taccgacttc atgaccaaat cataggcatc
     3361 gtcaatagtc acttcaggat tcagggcgct gtccaagtcc aatttccagt ctgcaaaggt
     3421 catcttttca agaccattta ccttggcaac atgcttgagg tatctctgag cgactggtgc
     3481 aaagtccttc atgatgaggt caatctggcg gtcaaacatg acacggtcca cttcttgttc
     3541 agctagaaga tagtcaaaga cagagtcgta tcccttcata tcagccaaga gtttttcaga
     3601 cttgacctga gccagatagg ctgctgcagc cgtattttgg tgcttacgaa gtccctctga
     3661 gaaggaacgg aaggatttct cacgaacctc agcatcctca tggttttggt agaaattctc
     3721 ataggtcaca aagctgtttt tgtaggtctt gccatgggct tcaaagtcag ccatttcaaa
     3781 atccccagct cgcatcttag tataaatgtc ctgcggactg tagaaaactt caccgagatt
     3841 tgtcaaggcc ttctccacat ctgcccctaa gtagtgggct tttttgattt tagcctgacg
     3901 aatggcagct gttaaatgtg gcaatttacc caaacgatcc aagacttcct cgtctgctgc
     3961 caccaaggca tcgtcaaaaa aggtcaaggc tacgctggca tctgtttcaa attccatccc
     4021 agcttgagca atatttgcaa attcgtcatt gctatagtcc gtcgtctgag gcataaaacc
     4081 atagttgcca atatggctca tctgaatgta gatctgttcc aattccgcaa aggccttctc
     4141 gaaatcctca aaagtgtgaa gattgccctt gtaatcacgg ctaaactggt tgatgtcttc
     4201 gcgagctttc tcgattgcac gcaagaaatc ctcacggtct tggtatagag ctgttaaatc
     4261 ccaaagttcc ttctctggaa attctgaacg gtgtttttgt tccattttct tcctcttatt
     4321 tctctaattc tactaaaaca ctaagggctg ataaggcgta aagcggtgct gtttctgctc
     4381 ttagaatgcg agggccaagt cctgccaaaa cagctccctt agcttcaaaa ttttcaattt
     4441 ccgcaggtga cagaccacct tctggaccaa agataaagag caatttggct cctttctcga
     4501 gtccagtaac tgcttttata agagcagcgg cttctccttc tttggccgcc tcttcatagg
     4561 ctactaagat gcggtcaaac tggtcaagtt gggctagaaa gtctgcttta ttctcgaaaa
     4621 gatggatact tggaacaaca ttacgcttac tttgctctgc tgctccaaga gcaatttttt
     4681 ctagtttttc aaccttttta cccaatttct tgccatccca cttagcaacg gaccaatcgg
     4741 cagggaaggc ccaaatctgg ctggcaccta gttccgtcac cttttgagtg ataaactcca
     4801 acttatctcc cttgggaaat ccagatgcga tggtcacttg gactggtagt tccacattgt
     4861 catttaattc ttggaccaac tcaaactgac gattttccat atccagcacg cgcgccaagc
     4921 gcttgatgcc atcatcaaag actaaggtaa cctcatcctc ttctttcaag cgcataacct
     4981 gaaacatatg cttgctggtt tccctgtcct cgatggtgac aggagagact gcactcccct
     5041 tgacaaaata ctgttgcatg ctagcctcca atcacacctg aaatatcctt ggtcttcttg
     5101 aagacgcagg cattccattc cccttgaatc atatgggttt caaggaaaaa tccagctgac
     5161 tcagccgact ggcgcaccat gtcccacttg tccttgataa tgccactcat gatgaggtag
     5221 ccttcatcct ttaccaagcg ataagcatcc tccgtcagat gaatgagaat atccgccaag
     5281 atattagcca cgatgacatc tgcttcaatc tcaactccct taagcaagtc tcctgctgct
     5341 acatggatat tttccatacc agtgttgagc tcaatatttt cctgagcgac acgaaccgcc
     5401 acatcatcca agtcataggc gaaaatctcc ttggcaccta ggagtgagct tgcaatagaa
     5461 agaacccctg aaccagtccc cacatctagc accgtttcgc caccacgaag aacctgttcc
     5521 aaggcaaaaa ggctcatctt ggtagttggg tgggttccag taccaaaagc catgccagga
     5581 tccagcttga taattttttc tcccgcagtc gcctcatagt ctgtccaaga gggaacgatg
     5641 gtcaaatcat gagtgatgcg agcaggttca tagtatttct tccagttgtc tgcccagtct
     5701 tcctcagcca aggcagtcgt acctattttt aactctccca aatccataaa atccgtcaat
     5761 tctgctaagc gagcctgcaa gtccgcctca accactgtca tatccaccgt gtcagggtag
     5821 taggctgtca ctacgatttc ttcttgctgc tccacctctg ggaaaatctc gccaaaacga
     5881 tccacatttc ccatatagtc catgctgtct tcaatcgcga ctccttgcgc tcccagctca
     5941 atcaagagat tggaaaccaa ctcctctccc tcacgcttca ctgtaacttt taactcttgc
     6001 catgtttcca ttattaagat accaagcccg taaaacacaa aaccaaaata ggaaattctc
     6061 tgaagacgct tgtgtctaag agaagtttat ctttttggca cagtgtttag ggcgggttca
     6121 gtttagaaat gtaactgaac catcctttct aatcacttac ttttaaataa tcttttaatc
     6181 tctcttgcaa ctgaggcaca acttgactgg aactaagaaa ttcctcaaca ttcatcagct
     6241 gatagccctg tccttcatct ccgaagatga tattgtcaaa ttgttcttgt cttagctgac
     6301 ccaccataaa gaccgatttc ttgcctttaa aaattacgct aggataaatc ttgctccaaa
     6361 gcagacagtc ttcatctaaa tgaattccca gttcctcata aacttcacgc cgagcgcatt
     6421 caaaagggct ttcgtcccct tcacggccac cacctggcaa ctcccacatg ttgggacaag
     6481 ggatatcgtc cttgtcatca cgcaaaatcg tcagtagctt atccccacaa aagagggcaa
     6541 tcttgcaacc ttggaaatca gaaatttcta gttccatctt cagttccttc taacatttcc
     6601 ttttccagct ctgctaacaa gttttcataa tagcgtttct cgtccctcaa cattcgacta
     6661 ctatccattt tctgtctagc aatcttgaga gccttacgag ttcgatctac atctttcttc
     6721 acctttaatt gataccaggc ttgtatcact tgaagattgg acagtttgag agacagaaac
     6781 gatttgacct gtcgaatact agcatattgc tccgcttgct caaaatctcc ttccaacaag
     6841 gcgatatgaa gcagggatag ttgggcaact gtctgcatca tcggagtagt tgtcctctca
     6901 agtaatgctt gaaactgctg tttagctact tcttccttcc cttccaaaat ggaaacttca
     6961 ccttgcatac ctaatacacc atccgcaaaa ctccctcgtg catcctcagg aactgcttga
     7021 acaaagtctt tcaaatcata ttcttgagga gccagcaaag tttgagcaga atgtctcaat
     7081 accaggtagg cgtatttggt attttcaggg tgttgtagta attcccaaat ttttgctcca
     7141 tcggtgatgt cgactggcaa aatgttattt aggaagaaag ataaattaag aaaaatccaa
     7201 gtccctgcaa aataccagct tcttgtcaaa aatccaaaca ctatcgccaa taatatcaag
     7261 ccgagatgaa ccatcaagcc tcctgaaagc atcaggatga ttctttgatc gctttcatcc
     7321 tcttttaaac caatgtattg agcaccaaca tttttcagaa tggctgttct actaagatga
     7381 aacctgcctg actttttggt caaaataaaa tgtcctaatc caaaagccac cagccgatag
     7441 cctgtcaagt agccacaaaa agcatgaccc agctcatgaa gaataaagat taaatacatg
     7501 cttagaagag cgaaggcata accaaaagta aaggctaaaa ctgcggaata ccccaactct
     7561 gcaaatgcga ttgttccaca agcaaaagct agcataataa agacaacagc tagcacataa
     7621 accaaataag tcccaatttt cttcataaca cctccaacca actcctagta tcttggataa
     7681 ggataaaatt ctccctcttc caagccaact tttccttctt caaagacttc ttggttccat
     7741 tccatgacaa attcctctgc ttctgggtct tccaaaaagt ccatgaggac atctagccca
     7801 acctcagcag tatctttaag gaaaagcaca aaataagcta aaaattcacg ggaaaatcct
     7861 tttttaggca ggtaaggaat aacagtcaaa tagtcttcct cattgactgt tgacttagca
     7921 ggattgtaga aaaggaccgc ttcctcaaaa agaatgtcat ctgatgaaac ctctccgtct
     7981 tcatccacca tctccacacc tgcagcattt tgcgcttcca atagaaaact cacttctacc
     8041 gcatggttgc gtttgtccca gctaatctca aagtcaaagg gaaagttctt gtccaactct
     8101 tcctctaaaa catctaaaaa tccgtatgtt gccattttgt cctctttcta tgcgactctt
     8161 taatcgcccc gattgctcgg aaatatgcta aaatagatac taccatctta ccacaaaatt
     8221 attttatgtc ctaattatac catattacct catttaaacc cttggtatca gtgattttct
     8281 taaaagtctg atttcttcat ttctcataaa aatcaatata aaaagccctc gaaagggcta
     8341 ataaatctat aaaatcaata ggcgagtaac tagcacaagt ggacgtgctt ttttattgac
     8401 tattaccacg ataccacgct taatcttagg cttgaacttt cttatctgca atagcgtctg
     8461 tcaaagtctg agaaaagtta agtcccattt ctcgtcccaa cttatctgcc cattttggta
     8521 tggtcaaagt ctttttaatg ggttcctgac ttcctaggta ttctgataca tcaacagata
     8581 ccatagaaat aaaagattta tcaaggtcat aggttgacac gaaatcttca tcatctttaa
     8641 aaggatcatt atcaattaaa gacaagctat tgatatctga tggctgaggt aactctccat
     8701 cactctctat caaatctgca acagttatcc ctagccactc cgaccccata gccaaagcct
     8761 cagaaatccc ctctccttgt gtagctgagt attcaaaatc tgggaaatgg acaaaataag
     8821 tcgcttctgt tccgtctgtg tcgtcataat aaaataaagc tggatacgta actaacattt
     8881 cactacctcc atatcaaaaa gcagggactg aattttacaa cccagcttgc tttcctatcc
     8941 ctctttcagt gtacttattc agctcaccat gaaggattgt gataggtctt tccccttgct
     9001 tctcgatttt aatatgggag cctttaccgc ctctagtctt tatccaacca tgggccgtaa
     9061 agagtttaac catctctttt tgtgtcatag gcatagcgct tttacctcct gacaacacca
     9121 ttataacacg tgttacacgt attgtaaagg agtgatactt attattctat tatacataaa
     9181 agcccctaga tgtggttcta agggaaacca atttattcag acctattttt ctaatgagta
     9241 gtaaaaactg cttctttatc gagcaattca tcatctgtat agtcaattgt aaaagtatct
     9301 cgatctaaga cagattgagg cggagttgaa tgaatcatag gaccactgcg tactctatat
     9361 tttttatctc caatttttac aaactgatac tcttcgaaaa tcaaattcaa accacgtcaa
     9421 cgtcgcctt
//
LOCUS       DAVBCW010000077         9023 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVBCW010000077 DAVBCW010000000
VERSION     DAVBCW010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9023
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.077"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            221..2830
                     /locus_tag="VQ514_001534"
     CDS             221..2830
                     /locus_tag="VQ514_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HET2500279.1"
                     /translation="MDYNFNLEHPFFFTNNDYSTDTSIKYQVSLPFNWHEVMNNDEWV
                     YQYPIGKFVERQGWKIHISSEYNSSHELLQDVAKICHEMRIPFKHLSTEDKFIMRNGK
                     LVSRGFSGKFITCYPNQNELESVLQRLESALKQYNGPYILSDKRWDEAPIYLRYGVFR
                     PSRDDEKKVAIDELIVGDEVVKDERLPVFKIPKGIVPPDFLNKWLDKKDKKQGDFPFI
                     IDNAIRFSNSGGIYNARLKEDGKKIILKEARPYTGLGFDGTYSSEKLASECKALKILN
                     EWSETPKIYWHGKIWEHTFLGIEHMKGVPLNRWVTNNFPLYEVVDKTKDYLLRVSKIV
                     EKLIDLTNKFHSENVYHQDLHLGNILVKDEDEISIIDWEQAVFSNDEKVVHKVAAPGF
                     RAWRETLPSEIDWYGIRQIAHYLYMPLVTTSDLTYNYVSQTRIEGKKLFESLGYTREH
                     IDYVESLLSYLDSKCPQIENISRKKVLKPMHEIRTIESEQDIQDFIIKLLRGFTLTYG
                     QWRKEFQSRFFPVHYYGLNFNQGIAFSDLAILWSYQQLAKKVKNFKFDDYYEIRTQVI
                     NEAVNNFKKSSLSGLFDGKIGTIWLIYEFGEIDRAVELFTTHFIEIFENSQNKNLYSG
                     QAGILLVGLYFLSKGEIDNKLGEEILIRLREYTLNYIENPETFCKVGASDVQSNDPYE
                     NFGGLLYGHAGVAWLFGEAYKLTGESIYKNGLELAVDKELVAYKVDSNNSLQYSQGHR
                     LLPYLATGSAGLLLLINRNKEILSSKYLKYLTSLERATDVVFCVLPGLFNGFCGLEVA
                     NNIYSDIDDNFSGQKKLIEQLYRYLCVIEEGFVIAGDNGLKITTDIASGFAGVAIGLV
                     SIMDNKLTILPQI"
     gene            2863..4803
                     /locus_tag="VQ514_001535"
     CDS             2863..4803
                     /locus_tag="VQ514_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET2500280.1"
                     /translation="MNQSNEIFLNTIQEKTHYKRSISSSFQELKKWNSLIFRRGRYWY
                     QFDNWSIPNTGVIKEWYVEDNDWIPKVSSSSLDNSNSLKIVFDVEKYTLPDDSILQDV
                     RISSDESYIVLVVSSLRSTNLIGIKKYTMEELFIIEDISVESSFYLGKFGVMYTRSKE
                     YGRPSKLFYKSFDSFTEEELFEENECSFRLKIVHIDSNNCFVKSVDFQKGRIFLYSFD
                     RTGFVRHSYTETVAPTPRDIALFSTEKAQYFLGLSSTEEKKDQLILRETSSGDRVSIT
                     IPYQDRARRVHCIGRYILLDCSNAANSVFYLATFKNNSLRELSVNKITFDEKTTLYEN
                     SFSDRVLLFLERRTFYEKILSFDLIENTLKTEFERPIIKSNNTKYFSKVIWTKKDSYD
                     VSIPISLFWKSEDTDELPRRKKCILSVYGAYGKNDNSDLDEIMLSIIDAGFIYAIVHV
                     RGGGYLGGEWYRSGKALNKWNSIRDFIEGVNYLRENDVIDSKRLGLITSSAGGIIAGA
                     VLNEEKNLLQSILLFSPFINPYDTLQNPNDPLSKTEIAEWGDIRDPEVKAYIKSYSPM
                     QNIEKARDSNTVIVNILGEKDPYINNNEVIEWSKKLNSIGVKSLLYLNKAAGHGGFTP
                     SDVLLMIDTLNYFFEEVGRNNL"
     gene            4800..6407
                     /locus_tag="VQ514_001536"
     CDS             4800..6407
                     /locus_tag="VQ514_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2500281.1"
                     /translation="MNYLKFIKKTKLILMGILIFLSSFNGVLLSGIIVYAGSLNQTSS
                     FSDVLRFGAISILGWSAIYISNYYLEVTEASITKDINVKIKQGYFREQYLSSEMVKDY
                     SSIISVLSNDLRLIEENYFRQIFEIISSILLFIVSLSFMLYLNFLVSIIFIVLSALPI
                     IVPVFMKKMLSNSANEYSNSNAEYTHIIKEIFNGFKTLKSYSVTKEIISLSDKKLDKL
                     EDSTFNLKRSEVLSKLVAVLISGFCFLVPLVVGCYFVIYHKSLSFSELIGIFLANDKV
                     LGPIQSIAYSLNKINTTKDLRKPFLKYLSGEKNFIDAEHDNNGLYTSSIDEIHMKDVV
                     YSITPENKLSIDFSFKSPFRVLLTGTSGSGKTTILNLINGSLKPQKGYVNLLSHGKKS
                     SDSIPTVDQTPYIFDTTIRENVTLFQNEYFSDDQIIEVLKKVNLYEELEKIDILNYQC
                     GENGSNLSGGQKQKIALARALIRNNKVYLFDEISANLDNDNSNSIHDILFNLGISFIE
                     VSHHYDLNDKRYTDIYKLENGTLFKIK"
     gene            6422..7162
                     /locus_tag="VQ514_001537"
     CDS             6422..7162
                     /locus_tag="VQ514_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2500282.1"
                     /translation="MDKDYILKVKGLYHQFLLGNNKTLQVLKNVSLSASRGEFISILG
                     ISGSGKSTLLKCISSLLEPTSGEVILNGINPYKIRNAKLSSIRRNEVSFIFQAYNLIP
                     SLPVIENIALPLRLSQKKLTIKNVENLLKRMKFNAGLNDFVGTLSGGEQQKVAIARAV
                     IADSDIIFADEPTGALDSVSREVIFELLRELVGAGKCVIMVTHDIELASKTDRALILK
                     DGKIFKELHRPSGEELYKILEVQSTTEE"
     gene            7165..8484
                     /locus_tag="VQ514_001538"
     CDS             7165..8484
                     /locus_tag="VQ514_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500283.1"
                     /translation="MLRLVYYQFLHNKKQWLGVSPVIFVSSLVMGLAVNGVINVENNS
                     QVFVGLPDPKPIFMFPIVFGGVTLFFVLSNIINMLVEIFRDDYELLEVLGASLLQLSF
                     LVGGQIFIISSIISFIAYLCSIFVTSNYYYFLQYFFGENILPDIQFQTSAVGCIITVV
                     LISFLAFLSGCFYTFKKIRNRKSSKIRHVLSIVKRILLLAGFSVIWLLSLQQIFQDST
                     ILAKAQIIFNIVILDIVIIYQLSPFIQSCFIKLLSIIIFRNNFMFIASKWNLLYRKPY
                     IKSISAAITGAILLISSFQMISQNILSQFQDDSDLELKVAFIVYVGAPILIVLANIIS
                     IAFLSSHQERIEIQQFEILGTSNYQMVKIKVGEAIFLTFVTSLIAFLLNIKIMALIYY
                     SLEDVLIDDMNLLGLILPNFIVSIILFILIFITKSSYFIFKNAKIIS"
     gene            8618..8755
                     /locus_tag="VQ514_001539"
     CDS             8618..8755
                     /locus_tag="VQ514_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
                     /protein_id="HET2500284.1"
                     /translation="MAEEVLNLQLVSVQVDETDEVDGMRFSTFSTNRCGNWSAFSWEN
                     C"
BASE COUNT         3096 a         1116 c         1709 g         3102 t
ORIGIN      
        1 cgttgcggta actggagtgc ttttagttgg gaaaattgtt aattcattta aattgtaaac
       61 tttagagatt ctctggagtt atggatgtcg ttccaatatg tgcacgttgg aatgttagtg
      121 cttatatggg gagaatccaa aggattctcc ctttttattt aaataagtct gtctagctat
      181 agatatatta agtttgataa aatttcttgg agattttgtt atggattata atttcaattt
      241 ggagcatcct ttctttttca ctaataatga ttatagtaca gatactagca taaagtacca
      301 agtaagtctg ccttttaatt ggcacgaagt aatgaataat gatgagtggg tttatcaata
      361 ccctattgga aaatttgttg aaagacaagg gtggaaaata cacattagtt cagagtataa
      421 cagttcacat gaattgttac aagatgtagc aaaaatttgc catgaaatgc gaattccatt
      481 taaacatctt agtacagaag acaaatttat catgcgaaac gggaaattgg ttagtcgagg
      541 attttctggt aaatttatta catgttatcc aaatcaaaat gaattagagt cggttttgca
      601 aaggttagaa agtgccttaa agcaatataa tgggccatat attttaagcg ataaacgttg
      661 ggatgaagca ccaatatatc tgaggtatgg tgtgtttagg ccgtcaaggg atgatgaaaa
      721 gaaggttgct attgatgaat taattgttgg cgatgaagtt gttaaagatg aacgtttacc
      781 agtatttaaa attccaaaag gtatagtgcc ccctgacttt ttgaataaat ggcttgacaa
      841 gaaagataaa aaacagggag actttccctt tattatagat aatgcaatta gattttcaaa
      901 cagtggtggg atatataatg ctaggctaaa agaagatggg aaaaaaataa ttttaaaaga
      961 ggctagaccg tatacaggtc taggatttga tggtacttat tcttcagaga aattagcttc
     1021 ggagtgcaaa gcactaaaaa ttctaaatga atggagtgag acgcctaaaa tatactggca
     1081 cggtaagatc tgggagcaca ccttcttagg aattgaacat atgaagggcg ttccactaaa
     1141 tagatgggtt accaataatt ttcctttata tgaagttgtt gataagacca aagattatct
     1201 attgagagtt tcaaagatag tagaaaaact catcgattta acgaataagt ttcattctga
     1261 aaatgtatat catcaagatt tacatttagg aaatatcttg gttaaggatg aagatgagat
     1321 tagtataata gactgggaac aggctgtctt ttcaaatgat gaaaaagttg tacataaagt
     1381 tgcagcccct ggttttagag catggcgaga aacacttccc agtgagatag attggtatgg
     1441 tattagacaa atagctcatt atttatacat gccactagta actacatcgg atttgacata
     1501 caactatgta agtcaaacca ggatagaagg aaaaaaacta tttgagtctt tgggttatac
     1561 aagagaacat atagattatg tagaaagttt attgtcatat ctagattcta aatgtccaca
     1621 gattgagaat atttccagga aaaaagtttt aaaacccatg catgagatta gaactatcga
     1681 gagtgagcaa gatatacaag attttattat aaaattattg agagggttta cgctaactta
     1741 tggacagtgg cgtaaagaat ttcaatcaag attcttccca gttcattatt atggtctgaa
     1801 ttttaatcaa ggaattgcgt tttcagattt agctatcctt tggagttatc aacaactagc
     1861 taagaaagta aaaaatttca agtttgatga ctattatgag attcgaacac aggttattaa
     1921 tgaggcagta aataatttta agaaatcgtc cctttctggt ctttttgatg gtaaaattgg
     1981 tacaatctgg ctcatttatg aatttgggga gatagatcgg gcagttgaac tttttacaac
     2041 tcattttata gagatatttg agaattcaca aaataaaaat ttatatagtg ggcaggctgg
     2101 aattttactg gttggactgt attttcttag taaaggggaa attgataata aactaggaga
     2161 agaaatacta attaggctta gagaatatac tttaaattat atagaaaatc cagaaacttt
     2221 ttgtaaagtt ggcgctagtg atgttcagag taatgatcct tatgagaatt ttggtggatt
     2281 actatatggt cacgcagggg tagcttggct cttcggtgaa gcttataaat taactggtga
     2341 aagtatctat aaaaatggtc tagaattggc tgtagataaa gaattagttg cttataaagt
     2401 agatagtaat aattcattgc agtacagcca aggtcataga ttactaccat atctagcaac
     2461 tggaagtgct ggcctgcttt tacttattaa tagaaataaa gaaatcttgt caagcaaata
     2521 cttaaaatat ttaacttctt tagagagggc aactgatgta gttttttgtg tattaccagg
     2581 tttatttaac ggattttgtg gtcttgaagt tgctaataat atatatagtg atatcgatga
     2641 taacttttca ggacaaaaga aattaattga acaactttat agatatttgt gtgtaattga
     2701 agaaggattt gttattgcgg gagataatgg tttaaagata acaacagata ttgcttcggg
     2761 atttgcgggt gttgcaattg gattggtatc aataatggat aataaattga cgattcttcc
     2821 tcaaatataa tatttagata ccattattaa ggggtcatac ttgtgaatca gagtaatgaa
     2881 atatttttaa atacaataca ggaaaaaaca cactataaga ggtctatctc ttcatcattt
     2941 caggaattaa aaaaatggaa ttctttgata tttaggagag ggcgatattg gtaccaattt
     3001 gataattgga gtatacccaa tactggtgta ataaaggaat ggtatgtcga agataatgac
     3061 tggattccta aggttagttc ttccagttta gacaattcaa attcacttaa aattgtcttt
     3121 gatgtggaaa aatatacttt gccagatgat agtatcttac aggatgttag gatttcatct
     3181 gatgagagtt atatagtttt ggttgtatcc tcactgcgta gcactaattt aattggaata
     3241 aaaaaatata ctatggaaga gttatttatc attgaagata tttcagtaga aagttcgttc
     3301 tatttgggta aattcggtgt tatgtatact cgctccaaag agtacgggag gccaagtaag
     3361 ttattctata aatcttttga tagctttaca gaagaggaac tgtttgaaga aaacgaatgc
     3421 tcttttcgat taaaaatagt ccacattgat agcaataatt gttttgtaaa gtcagtagac
     3481 tttcagaaag gcaggatttt tctatacagt ttcgaccgga cgggttttgt tagacactct
     3541 tacacagaaa cggtagcccc aacacctaga gatatcgctc tatttagtac agaaaaagca
     3601 cagtactttc ttgggctctc ttcaactgaa gaaaagaaag atcaactaat tctaagggaa
     3661 acttcttcgg gagacagagt ttctattact ataccatatc aagatagggc tagaagagtt
     3721 cactgtatag gacgttatat ccttttagac tgttcaaacg cagcgaattc cgtcttttat
     3781 cttgctacat ttaaaaataa tagtttgagg gagttgagtg ttaataagat aacatttgat
     3841 gaaaagacaa cactttatga aaattcattt tctgatcgtg ttcttctgtt tttagagagg
     3901 agaacttttt acgaaaaaat acttagtttt gatttaatag aaaatacatt aaaaactgaa
     3961 tttgaaagac ccatcataaa atcgaataat actaaatatt tttctaaggt gatttggact
     4021 aaaaaagaca gttatgatgt aagcataccg atttctttat tttggaaaag tgaagatacg
     4081 gatgagctcc caagaagaaa aaaatgtatt ctaagtgtct atggggctta tggtaagaat
     4141 gataattctg atttagatga aattatgtta tctataatag atgcaggttt tatttatgct
     4201 atagttcatg taagaggtgg aggctatttg ggtggtgaat ggtatcgctc aggaaaggcg
     4261 ttaaacaaat ggaattctat cagagatttt attgagggag taaattattt aagggaaaat
     4321 gatgtaattg acagtaagcg attaggttta ataacttcta gtgcaggtgg aataattgct
     4381 ggtgcggtgt tgaacgagga aaaaaattta ttgcaaagca ttctcttatt ttcgccattt
     4441 ataaatcctt atgatacact tcagaatcca aatgatcctc tttctaagac cgaaatagca
     4501 gagtggggag atattagaga ccctgaagta aaggcttata taaagtcata ttcgcctatg
     4561 caaaatattg aaaaggcacg agactcaaat actgttatag ttaatatttt gggtgagaaa
     4621 gacccatata ttaataataa cgaagtgata gagtggtcaa agaaattaaa ttctattgga
     4681 gttaaaagtt tgttgtattt aaacaaagca gctggtcatg gaggttttac tccatcagat
     4741 gttctcttaa tgattgatac tttaaattat ttctttgaag aagtgggaag gaataactta
     4801 tgaattattt aaaatttata aaaaagacta aacttatatt aatggggatt ttaatatttc
     4861 tatcttcgtt taatggtgtc ttgctctcag gaattattgt atatgcaggt agtttaaatc
     4921 agacttcctc attttctgac gttctaagat ttggtgctat aagtattctg ggttggtcag
     4981 caatctatat atcaaattac tatttagaag taacggaagc atcaataacg aaagatataa
     5041 atgtaaaaat taaacaagga tattttagag aacagtatct ttcttctgaa atggttaaag
     5101 attactcatc tattatttca gttttgtcaa atgatttgag attaatcgaa gaaaactatt
     5161 ttagacaaat ttttgaaata atttcttcaa tattgttgtt catcgtctct ctaagtttta
     5221 tgctttattt aaacttccta gtttcaataa tatttattgt attatcagca ttgccgatta
     5281 tagtacctgt ctttatgaag aagatgttgt ctaattcagc taatgagtac tctaacagca
     5341 atgcagagta tactcacata ataaaagaaa tttttaatgg ttttaagaca ttaaaatctt
     5401 attctgttac taaagaaata attagtttgt cggataaaaa gttggataaa ctagaagatt
     5461 ctacttttaa tttgaaacga tcagaggttc tttcaaaatt ggttgcagta ctaatttcag
     5521 gtttttgctt tctagttccc ttggttgtag gatgttattt tgtaatttat cataaaagcc
     5581 tttcttttag tgaactgata ggtattttct tagcaaacga taaagtttta gggccgatac
     5641 aatcaattgc ctattcatta aataagataa atacaaccaa agatttaagg aaaccgtttt
     5701 taaaatactt aagtggagag aagaatttta tagacgctga acatgataat aacggactgt
     5761 atacttcatc aatagatgag atacacatga aagatgttgt atattctatt acaccagaaa
     5821 ataaattaag tattgacttc tcatttaagt caccatttag ggtattatta acaggaactt
     5881 ctggtagtgg gaaaacaacg attttaaatt taattaatgg ttctttaaag ccacaaaaag
     5941 gttatgtaaa tttgttatca catgggaaaa agagttcaga ttcaatacca acagttgatc
     6001 agacaccata tatttttgac actactattc gtgagaacgt aactttattt caaaatgaat
     6061 atttttcaga tgatcagata attgaggtgt taaaaaaggt aaatctatat gaagaattag
     6121 aaaagataga tatactaaat tatcaatgtg gtgaaaatgg tagtaatttg tctggaggtc
     6181 aaaaacaaaa aatagcttta gctagagctc tgattagaaa taataaagtg tacttatttg
     6241 acgaaatatc agctaattta gataatgata attcaaattc catacatgat attctgttca
     6301 atttaggtat ttcatttatt gaagtttcac atcattatga cttaaatgac aagagataca
     6361 ctgatatata taaattggaa aatggaacac ttttcaaaat taagtgattg gaggcaagaa
     6421 tatggataaa gattatatat taaaagtgaa agggctgtat catcaatttt tactaggaaa
     6481 taataaaacg ttgcaagtgc tgaaaaatgt ttctctttct gcttcgagag gagaatttat
     6541 aagtattcta ggaattagtg gttctggaaa gtcaacttta ttaaaatgta tttctagttt
     6601 gcttgaacca acaagtgggg aagtaatttt aaatggaatc aacccctata aaatcagaaa
     6661 tgcaaaattg tcaagtataa gacgtaacga agtatctttt atatttcaag catacaattt
     6721 aataccttcc ctgccggtaa tagaaaatat agcacttcct ttgcgattat cacaaaaaaa
     6781 attaactatt aaaaatgtag aaaacttact caaaagaatg aagtttaatg ctggcttaaa
     6841 cgattttgtt ggaactctgt ctggtggaga acaacagaag gttgctatag ctagagcggt
     6901 tattgctgat agtgatataa tatttgctga tgagccaact ggggctttag acagcgtttc
     6961 tcgtgaagta atttttgaat tattgagaga gttagtaggg gcgggtaagt gtgtaattat
     7021 ggtaacgcac gatatagaat tggcctcgaa aactgatcgt gcattaatat tgaaagatgg
     7081 aaaaattttc aaagaacttc atagacctag cggggaagag ttgtataaaa tcttagaggt
     7141 acaatcaact acggaggaat agagatgctc agattagttt actatcaatt tttacataat
     7201 aagaaacaat ggttaggcgt ttcaccagta atttttgttt cgagtttagt tatgggattg
     7261 gctgttaatg gggtaattaa tgttgagaat aattcgcaag tatttgtcgg acttccagat
     7321 cctaaaccaa tttttatgtt tccgatagta ttcggaggag taacattatt ctttgtgctc
     7381 tctaacataa taaatatgtt ggttgaaatt tttagagatg attacgaact tctggaggtg
     7441 ttgggagcaa gtctccttca actatcattt cttgtggggg gacagatttt tatcatttcc
     7501 tcgattatta gttttattgc atatctatgc tctatatttg ttacgtcaaa ttattattat
     7561 tttttacaat actttttcgg ggaaaatata ttacctgata tccaatttca aacgagtgct
     7621 gttggatgta tcattacagt agtgttgatt tcatttttag cctttctaag tggttgcttt
     7681 tatacgttca agaagattag aaataggaag agttctaaaa taaggcatgt tctttcaata
     7741 gtaaaaagaa tactgctatt ggctggtttt agtgttatct ggttgctttc tttgcaacaa
     7801 attttccaag atagcacaat tttagcgaag gcccagataa tattcaatat tgtaatctta
     7861 gatattgtga ttatttatca attatctcct tttatacagt catgttttat caaactactt
     7921 tctataatta tatttagaaa taattttatg ttcattgctt cgaagtggaa tttactatat
     7981 cgtaagcctt atataaaatc tatcagtgct gcaattacag gagctatatt actaatttct
     8041 agttttcaaa tgatttctca gaatatacta tctcaatttc aagatgacag tgatttagag
     8101 ctaaaagtgg catttattgt ctatgtagga gcaccaattt tgatagtact tgcaaatatt
     8161 atttcaattg catttttgtc atcgcatcaa gaaaggattg agattcagca atttgaaatt
     8221 ttaggaactt ctaattacca gatggttaag attaaagttg gtgaggctat atttttgaca
     8281 tttgttactt cattaattgc atttttactt aacattaaaa ttatggcttt aatatactac
     8341 tctttagagg atgttttaat agatgatatg aatcttttgg ggttaatact tccaaatttt
     8401 atagtgtcta ttattttatt tattttaata tttattacta aaagttccta ttttatcttt
     8461 aagaatgcaa aaattatttc ttaaaaattt gtaagttttc gctatataaa atagttaaat
     8521 ctattttgta tcattgcttg cattttactg gtggagagaa tataatgagg gtggataaag
     8581 tacttaatcc caaaaattta aataggagga gctagctatg gcagaagaag tattaaatct
     8641 tcaattagta tctgttcaag ttgacgaaac agatgaagtt gatggaatgc gatttagtac
     8701 attcagtaca aaccgttgcg gtaactggag tgcttttagt tgggaaaatt gttaattcat
     8761 ttaaattgta aactttaggg attctctgaa gttatggatg tcgttccaat atgtgtacgt
     8821 tggaatgtta gtgcttatat ggggggaaca gaatcctctc ttgattgaag acaagttagc
     8881 cattaggctg gtttgtctct ttgtcaactg tagtgggttg atataatagt attagtgagt
     8941 gggataaaag tttcatttag tttattcagt acaaatttaa cgggtcaaga tttattgaac
     9001 tgcaccccaa tagttagaca gaa
//
LOCUS       DAVBCW010000078         9006 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVBCW010000078 DAVBCW010000000
VERSION     DAVBCW010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9006
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.078"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(69..464)
                     /locus_tag="VQ514_001540"
     CDS             complement(69..464)
                     /locus_tag="VQ514_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HET2500285.1"
                     /translation="MYNKVIMIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(542..1303)
                     /locus_tag="VQ514_001541"
     CDS             complement(542..1303)
                     /locus_tag="VQ514_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET2500286.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMIHKK"
     gene            complement(1336..1962)
                     /locus_tag="VQ514_001542"
     CDS             complement(1336..1962)
                     /locus_tag="VQ514_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HET2500287.1"
                     /translation="MIFTYNKEHVSDVLMVIVKNSGDAKLNVERKGKVARVFLKENGE
                     TVAWNIFEVSSLFEIAERGQVFLSDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKIVQIVAGAPNARVGLKTIVALPGAMMPKGNFIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            complement(1978..2295)
                     /locus_tag="VQ514_001543"
     CDS             complement(1978..2295)
                     /locus_tag="VQ514_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET2500288.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKEQINDFLA
                     GLK"
     gene            complement(2292..2591)
                     /locus_tag="VQ514_001544"
     CDS             complement(2292..2591)
                     /locus_tag="VQ514_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HET2500289.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDHFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            complement(2876..3067)
                     /locus_tag="VQ514_001545"
     CDS             complement(2876..3067)
                     /locus_tag="VQ514_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HET2500290.1"
                     /translation="MYYSVDDVVSNAFKKRMILDSFFSFNCSGTMKVSTWVYDKGEWY
                     YVSSSGSMIANDWVKDNGK"
     gene            complement(3228..3902)
                     /locus_tag="VQ514_001546"
     CDS             complement(3228..3902)
                     /locus_tag="VQ514_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500291.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLIIRGDVAFGKL
                     EIVYAK"
     gene            complement(3908..4354)
                     /locus_tag="VQ514_001547"
     CDS             complement(3908..4354)
                     /locus_tag="VQ514_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2500292.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(4468..4971)
                     /locus_tag="VQ514_001548"
     CDS             complement(4468..4971)
                     /locus_tag="VQ514_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET2500293.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            complement(4968..5330)
                     /locus_tag="VQ514_001549"
     CDS             complement(4968..5330)
                     /locus_tag="VQ514_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HET2500294.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICGVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(5396..7255)
                     /locus_tag="VQ514_001550"
                     /pseudo
     CDS             complement(5396..7255)
                     /locus_tag="VQ514_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850459.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(7294..7503)
                     /locus_tag="VQ514_001551"
     CDS             complement(7294..7503)
                     /locus_tag="VQ514_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET2500295.1"
                     /translation="MELEASPVDKQAKFVRLTDKARSQMQQVKAFFERIDKQLMEDID
                     EDELLIFEKVLGQLQAKYQGNRRRE"
     gene            complement(7513..7743)
                     /locus_tag="VQ514_001552"
     CDS             complement(7513..7743)
                     /locus_tag="VQ514_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET2500296.1"
                     /translation="MDKPMLVFKRFGHQTYLMVQKEAKRCGIEFMGGPQGQVVRFWDN
                     REKNQDLVLIKDIEQELKITKSVASNLVKRIV"
     gene            complement(7867..8583)
                     /locus_tag="VQ514_001553"
     CDS             complement(7867..8583)
                     /locus_tag="VQ514_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2500297.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHVIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
BASE COUNT         2656 a         2111 c         1638 g         2601 t
ORIGIN      
        1 gctgctcaaa gcattgcttt gatgttgtgg ataaagctaa cgtggtttga agagattttc
       61 gaagagtatt aaaatggcaa ttcttcctct tccaagacta aatctgccaa atcttggcct
      121 gcattatttt cacgcatggc acgttgagca cgactttcca agagttggaa tcctgttaca
      181 agtacttcgg tcacgtagtt catttggcca tttttctcaa agcgacgggt acgcaattct
      241 ccatcaacgg aaatgagact acctttggtt gcgtagcttg ccaaagtttc tgctagtctg
      301 ccccatagga ccatattgac aaaatcagct tcacgttcac cgttttggtc tttgtaacga
      361 cggttcacag cgatagttgc tcgcgctacc gacttgtcat tgttggtttt gtgcaattct
      421 ggtgtagacg ttaaacgccc aatcatgata actttattat acatattttc ttcctcctac
      481 ttatctattc gtaggaaatc aaaaaaagtt acagaaattt gtaacttttc gaggaaattt
      541 cttatttttt atgaatcatg aaacctgtcg cctgttgatt ggccataatg gtcatatctg
      601 taatctgaac acggcgaggc tgactggtca catagactac tgtgtctgca atatcctgag
      661 cttgcaaggc ttctattcct tggtaaacgg acgcagctcg ctctttatca ccatgaaaac
      721 gaacagttga gaaatctgtt tcgacaatcc caggctgaat ggttgtcacc ttgatatccg
      781 ttgcgatggt atcaattcgc agtccatccg aaaaggtctt aaccgcagcc ttggtagctg
      841 agtaaacagc ggcaccagca taggcgtaga ttcctgcggt tgaccccata ttgataatat
      901 gaccttgatt gacttttatc attgctggca agaaacagcg agtaaccgcc atcagacctt
      961 taacattggt atccaacatg gtcaacatat ccaactcctc atagtcttga tagggagcca
     1021 agtcaagagc cagtccggcg ttgttgacca agatatcaat cctccctatc gtttctagaa
     1081 tatcagagca aacagtcttc accatgacca tatccgtcac atctagtgga aaagtccaga
     1141 cggtttgatt tggaaagctt actgcaaact ccgacttgag gatttctaat ctgtctatcc
     1201 gtcgtcctgt tagaacgaca tcctcaccct gctccagata agcacgcgca atcgcttcac
     1261 cgattcctga ggttgctccc gtaatcacta cattttttgc catcttattt ccttctatca
     1321 gatgctgaca agttcctagg cagtccagtg tttcgctggg tcgaacggtg ttccgacaac
     1381 ttggtcttct gataattcaa gcaccccacg tttttgcgga gcatttggca aatgcaattc
     1441 acgaggacta cacatcatcc caaaactctt ttcaccacga agttcgcctg ggaaaatgaa
     1501 attccctttt ggcatcatgg caccaggaag agccacaatg gttttcaacc caacacgcgc
     1561 attaggtgct cctgcaacga tttgcactat cttgtcactt gcgactgcaa cttggcagat
     1621 gttgaggtgg tcactgtctg gatgagctac catctcgaca atttcaccaa caacaaactt
     1681 aggttcctta tcattaacaa tttcttctgt aaaaccttcc gcctgtaatt cttggttcaa
     1741 acgagcgact tgctcatctg ataaaaagac ttgaccgcgc tctgcaattt caaacaagct
     1801 cgaaacctca aaaatgttcc aagccactgt ttccccattt tctttgagga aaacacgggc
     1861 taccttgcct ttgcgttcca cattcagttt ggcatctccg ctatttttca cgatgaccat
     1921 aaggacatca ctgacatgtt ctttgttata cgtaaaaatc attgtttcct ttttctccta
     1981 tttcaatcct gctaaaaaat cgttaatttg ctccttactt ttacggtcgc gattgacaaa
     2041 acgaccgatt tccttgtcct tttctagaac aacaaggcta ggaattccgt aaacatccca
     2101 gagtttggcc aaatccatat actgatctcg gtccattcga ataaaggtga actctggatt
     2161 ggtctcttca atctctggta aggcaggata aatataacga caatcgccac accagtctgc
     2221 cacaaaaagg aagaccttct tgcccgcttt ttccactaaa gatgctaatt cttctaaact
     2281 tgctggctgt atcataagac ttcctcctca tagactaggt cttcattttc atagacaaag
     2341 gtataatgac ggccatcctc aaaaatgacg ccaccaacca agctctccag actgctttcg
     2401 taaacttgaa cataaagggt cgcaatttcc cccatgtcgg aaaaatggtc tcgcacaatc
     2461 tctgtcaact cttcctgagt cttcatgagc ttacggtcat ctgcaacttt tttcgtagca
     2521 agagcaaggc ttccgatacc tagcagagcc aagcctgcca tccacatttt tttagctttc
     2581 ataccattca ttttaacaca aaaaaggctt caggacaaat gaggaagcag cagaaaagca
     2641 agtaaaaagc ctcttccttt aaggaaaagg acttcttata ctaagagcaa actagaaagc
     2701 tagccgcagg ctgctcaaaa cacggttttg aggttgtaga taaggcgacg ttgacgtggt
     2761 ttgaagagat tttcaaagag tattattctt attgccaggc acctaagttg ccaacgtagt
     2821 aaccatcagg tgtgtaggta ttgcgaagca tcttacctga tgaagccaga taatactact
     2881 tgccattgtc tttgacccaa tcattcgcaa tcatggaacc agaagaactt acataatacc
     2941 attctccctt gtcataaacc caagtactga ctttcatggt tcctgagcaa ttaaaggaaa
     3001 aaaaactgtc caatatcatt cgttttttaa aagcattcga cactacatca tctacgctgt
     3061 agtacataga tacatcaaac gtaaaagcca aaatatcgaa aaatatacaa cttttaaagg
     3121 cagcatttca ttttttaaag tctaacaacc atagaactga aatcagagca aggcatcaat
     3181 ttcctactct tagtacgcct attttgatgg ttgaagtgaa gattttttta tttagcgtag
     3241 acaatttcca acttcccaaa agccacatct ccacggataa tcaaggtttt gctggttggg
     3301 gctacaggag cgtcagcctt agctgcacca aaggaggttt ctactttcaa atctacacgc
     3361 cagtgttgtg gaacatagac ggttgcattc ccgaaggcca cttcaatatt taaagttgca
     3421 aaatcaccta gcatctctgc attatcatag tagattttag catccccaaa agcgacttcc
     3481 acttggtcat ctacgagatc ttgatcttgc ttatagaagg tcccgctacc aaaagcgact
     3541 tccttatccg tgacgactgt ttttttccca ttgtaccacc attttttttc attccagaac
     3601 ttacttgaat gcgtcagata accaacacca agtaccacca agatgctagc ccaaataaga
     3661 gaatgattgg taactggtaa caagtcataa gcgtaatttg caatgatgag cgccagtaaa
     3721 cctgtaaaaa ctgccgaagt gagatgacgt ctaaggatgg actcaatgga cttataagca
     3781 aaaaaaacaa tacctagtaa aggccatatt ttaccatcca aagaaggaat tccaaaattc
     3841 ccttgcagca agatccaagc tgctaaaacc aataaaacaa taccaaatgc tttctttttc
     3901 atgtttttta cctcatgttt cttagatttt cttttaggag ggattggtaa tgccgtgaga
     3961 catgaacctc cttgtgcgtc tgataaaagg aaatggtgcc cgttcctgaa aaggacttgt
     4021 ccactgagta aatctgacgg atgtttgcga tcgttgactt ggaaactcga ttaaaatagc
     4081 gaggcaagat agactccaac tcatagagct tgaggcgaac ctcataagct tccttctggt
     4141 tatgagcgta gatcttgctc ccttctgttt caaagaagag aatttctgac aggtctagat
     4201 aatattcacc tgtccccttg taaaaagtca acctcggagc ctttgactct tgcaagagcc
     4261 gttgcaagtc tgcaatttca tctgtcaaag tgggggtctt gatgacaatt tcagtctcct
     4321 ctaaattgcc gtcaatctca attcgtaact tcatcacatc tcctttctga ctctactata
     4381 tcaaagctaa gttaggaata caagggcaaa aaagcaagtg gtcaatttga agcagtaagt
     4441 ggttgtagga ctagcttaac ttacatgtca gaataggaag aaaaggaatc cccacaccaa
     4501 accacaagca tagccaacca agacatcctt gggataatga acacccccta aaaccctcac
     4561 caaccccagc aaagctgaaa agatcaagca tactaagcca ataggtagac tagcatgcag
     4621 acaggccatg gagatgatag ttgccgaaaa gacatggcga ctgggcatcg accgtcccga
     4681 actatcctta tcaagaaggg gacagatatc ccaagtttca taaggtctcg ggtgattgat
     4741 tttcttacga aacagggaca aggccacaaa acctgatgca ggaataagca cataaacccc
     4801 aacttgctga ccaagtccta gttgcaggta agtggtgact agcaagatca gatagaccag
     4861 gggcatggcg actgtcatca aacgattgaa actgcgtaat aggaataaaa gaattggacg
     4921 actagttagt ctggagctga tatttcgata ccattcttga taatttttca tttatgcttc
     4981 tcatactttt ctagaacttg ttgagatgtt ttccccttgg caagccaatc cactagcttg
     5041 tccaagcgac gtatttcctg catcagagga tcatcaattt cctcaatacg gacaccgcaa
     5101 atcttccctg taattgcaat tcgttcaggg acataatatg gtgcttgtct aaaaaaatct
     5161 ccgtaagtaa catccctatc cagacaagca agaacatcac tcacttcata ccctgtcaac
     5221 caactggtca cttggtgaac tgactctgcc tttccaccct ttcgctcaac ttttgcaatc
     5281 aaggctaagt agacagcagc aaatttcata ttgtataatt tctgactcat acttctctct
     5341 ttctatgtgt actagtataa ataagaaaaa gaaggccgtc aagccttctt ttgatttatt
     5401 cttctgcttc atcttctgta aattgactat tgtacaagtc agcgtagaag ctaccttgcg
     5461 ccatcagttc ctcatagttg ccttgctcga tgatatttcc atctttcatg accaagatca
     5521 agtctgcatt tcggatggtt gacaagcggt gggcaatgac aaaggatgtg cgtccttcca
     5581 tcaaacggtc catggctttt tggatcaatt cctctgtccg tgtgtcaaca gaagaagtcg
     5641 cctcatccaa aatcaaaagc ggtgcatcct taagaagggc acgagcaata gtcaatagtt
     5701 gtttttgtcc tacagacaag gtcacggtgt catccaagat ggtatcatag ccatctggca
     5761 aggtcataat aaagtggtga attcccacag ccttactagc ttccatcatt cgttcatcac
     5821 taatccctat ttgattatag atgagattgt ctcgaatagt tccttcaaag agccaggtat
     5881 cctgcaagac cattgaaaag gcatcatgca cttctgaacg cgtcatagcc ttggtatcca
     5941 caccatcaat gtgaatactt cccttatcaa tctcatagaa tttcatcaaa agattgacaa
     6001 tggttgtctt accagtccca gtcggcccaa caatggcaac cttttgacca gcatgagctg
     6061 tcgcagagaa gtcatagtct tgaacattga caccgtccac cagaatttct cctgctgaca
     6121 cgtcgtagaa acgtggaatc agattgacca gagttgattt accagaacct gttgacccaa
     6181 taaaggccac tgtttgacca gtttctgctt taaagctaac atgttcaata actgcctccg
     6241 aatttgccgc atagcggaag gtcacatcct taaactcgac ctgacctttg aagttttcat
     6301 cagtcagctg cacttgaaca gggttttgga tagaagaatg caaatataaa acttgattaa
     6361 tccgcttagc agagaccata gttcggggaa gaacgatgaa gagtgctccc atgagaagga
     6421 agcccatgac aacctacatg gcataagaca tgaaaacaat catgtcacta aagagaggca
     6481 gacgcgctat cggagcagcg tcgttaatca cataggcccc aatccagtaa atcgccacac
     6541 tcaaaccact tgaaatcccc atcatgatag gattcaaaat agccataaga cggttgacaa
     6601 acaaattcaa acgggtcaat tcatcattta ctgctgcaaa tttttcattt tgataatcct
     6661 ctgcattgta ggcacgaacg acacgaatac ctgttaaact ctcacgagtg atactgttca
     6721 gtttatctgt cagcccctga atcaaggact gttttggaaa ggctagcgtc atcaaaacgg
     6781 tcgtcatcag gacgttgaca atcactgcca caagtacggc ccagagccag tattctgaat
     6841 gacctaaaat cttcccaata gcccagatag ccataattgg accacgcgtt accacttgca
     6901 agcccatagt aatcaacatt tgaacttgag taatgtcatt ggtagtacgc gtcaagaggc
     6961 taggaattga aaatttctta atctctgtct gcgagtaatc caaaactcgg ttaaaaatat
     7021 cacttctcag cctactaata taaaaagccg ccactcggga tgcaaaaaat ccaactgcaa
     7081 ctacggacaa gaaggcaaga aagggcattc ccatcatcat gcttgccggc tgccacaact
     7141 catctaaatt agttccttga ctacctagca aatccgtaat tttcgagata taggtcggca
     7201 cttccaactc tagatagacc gaaaagcaag taaagagaat ggctagtaaa atcatccccc
     7261 attcttttct actaattctt ttggctaatt tctttattct ctcctcctat tcccttgata
     7321 ttttgcctgt agttgaccga gaactttctc aaaaatcagt aattcatctt catcaatgtc
     7381 ttccatcaac tgcttgtcta tgcgttcaaa aaaagcctta acctgttgca tctgagaacg
     7441 tgctttgtcc gtcagacgaa caaacttagc ctgcttatca acaggactcg cctccaattc
     7501 caccaaacca ttttacacta tacgcttaac cagattacta gcaacagact tggtaatttt
     7561 gagttcctgc tcgatatctt taatcaagac caagtcttgg tttttctcgc gattatccca
     7621 aaaacgcaca acctgacctt gcggcccacc cataaattca atgccgcaac gtttggcttc
     7681 cttttgcacc atcaggtaag tttgatgacc aaaacgctta aagactaaca tcggtttatc
     7741 cataatctcc cccttctaaa taaaaatagt tctctggaga ataattaaat ttctatgaga
     7801 actattttct tgattaaaaa aatcccaagt gattttctca cttaggatca tgttctatag
     7861 gttaaattaa aacccatcta cgttcgtata aatcttttgg acgtcttcgt cgtcttcaag
     7921 aacgctgtaa agtttttcaa aagtttcaag gtcttcgcct gacaattcca cttctgactg
     7981 aggaatcatt tccaattcag tcacttggaa ttcttcaata ccagactcac ggagggcaac
     8041 gatagccttg tgaaggtcag ttggcgctgt gtaaactgtg attgtacctt cttgtgcttc
     8101 tacgtcatcc acatccacat ccgcttcgag caattgctca aagactgcgt ctgcatcttc
     8161 acctccaaat acaataacac ctttgttgtc aaagaggtaa gaaacagaac ctgaagcgcc
     8221 catgtttccg ccgtttttac caaaggctgc acggacattg gctgctgtac ggttaacgtt
     8281 agaagtcaaa gtatccacaa ttagcataga gccatttggc ccaaaacctt cgtaacgtcc
     8341 ttctgtaaag gtttcgtctg tgtttccttt ggctttatca atcgctttat cgataacgtg
     8401 ttttggcact tgggcttgtt tagcacggtc gataacgaat ttcaaagctg agtttgattc
     8461 tggatctgga tcaccttttt tagctgctac atagatttct acaccaaatt ttgcatatac
     8521 tttagagttg gctccatctt tagccgtttt cttggctacg atattggccc atttacgtcc
     8581 cattaggaat ctcctttttt cacattttaa tctttcttat tataacacaa gtttttttga
     8641 ttttcactag aggaaatgga ttttattagc aaatcaagct aggatagtac tttacctgct
     8701 aagatggtct tgcctttcta tctttatcaa cagacactca tccacattca aaaaacaaac
     8761 tagaccatta tctgcaaata gaaagtttca gccaagtttg acaaagtcag ctcaaattac
     8821 tgtttgaagt ttgtagatat aagcgacaaa aacaatcata ctgcaccttt tgttgacagt
     8881 ctactccaga catatcatag ttccagtaaa tactttgcaa tccaacagtt cttataggcg
     8941 ctattgtatt ctaagaaatt aatagacgag tttctaagca aacctctaat actcaataaa
     9001 aatcaa
//
LOCUS       DAVBCW010000079         8979 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVBCW010000079 DAVBCW010000000
VERSION     DAVBCW010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8979
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.079"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            <1..82
                     /locus_tag="VQ514_001554"
     CDS             <1..82
                     /locus_tag="VQ514_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET2500298.1"
                     /translation="DYYNNKRIKVKLKGLSLVQYRTKSFG"
     gene            complement(198..1001)
                     /locus_tag="VQ514_001555"
     CDS             complement(198..1001)
                     /locus_tag="VQ514_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HET2500299.1"
                     /translation="MQSTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNTPFQQHSKY
                     FRDALVLDNAKILQRRSEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
     gene            complement(1278..2033)
                     /locus_tag="VQ514_001556"
     CDS             complement(1278..2033)
                     /locus_tag="VQ514_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HET2500300.1"
                     /translation="MSKHPHYELLNLIGYGLAKFDKLFIKEFQCSSKSEFYRYVVSLG
                     IAETTGVVKNRMDLFDPYFDNNRKGWWQKAEVYRFRKDLIDMMFGNEDVHSYAEIVKM
                     LLASEGKKTGITIVEKPIVRTKFKRLQETGMEAENYFILHFDKEEKFQGGQLTDARLY
                     GDGYDFQVDVQEYSYLAEVKGIRKSKGRVRLTAKEFEKVKEFQSDFILSLVTNLDDIP
                     KLVLIDNPLKHFEFKKNIIKNEIIEYRSVEDLY"
     gene            complement(2103..4754)
                     /locus_tag="VQ514_001557"
     CDS             complement(2103..4754)
                     /locus_tag="VQ514_001557"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HET2500301.1"
                     /translation="MSKELSSKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ISRYDLGRESFLTKVWEWKDEYSTTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPKFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTDDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNEAGEIYVGEEAPAGDGWKQDEDVLDTWFSS
                     ALWPFSTMGWPNTETTDFKRYYPNSTLVTGYDIIPFWVSRMIFQGIEFTGKSPFKNAL
                     IHGLIRDEEGRKMSKSLGNGIDPMDVIDKYGTDSLRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEEEKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQISEGSIVTAEYPTVNPAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIFLPLVDLLNVEEELARLEKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     ERDKQADYQAKYDATVARIDEMKKLVK"
     gene            complement(4747..5325)
                     /locus_tag="VQ514_001558"
     CDS             complement(4747..5325)
                     /locus_tag="VQ514_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET2500302.1"
                     /translation="MHLFIIGAPASGKMTIGQELSRLTDATLFYNHQAIDFALEIYQD
                     YTEEMWEFVRGITFSFLGASARNQRSVILTDVIDFSNQYQLMYLKQIQNLLDDYHQEI
                     LFVELETSLEECIRRNRTENRLKHKPLKRHIEVSEREILETAETLQLNSQYQPNELHH
                     YFKINNTNLSAEEAAKQIQNKINKIEKGHTHV"
     gene            complement(5325..5795)
                     /locus_tag="VQ514_001559"
     CDS             complement(5325..5795)
                     /locus_tag="VQ514_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2500303.1"
                     /translation="MARTELPDKIETERLVLRVRTVADAEDIFDYASLPEVAYPAGYG
                     IVVKGTDKIVGSVDFNHRHEDDVLEIGYTLHPDYWGRGYVPEAARALIDLAFKDLGLH
                     KIELTCFGYNLQSQRVAEKLGFTLEARIRDRKDAQGNCCDDLRYALLKSEWEVI"
     gene            complement(5782..6360)
                     /locus_tag="VQ514_001560"
     CDS             complement(5782..6360)
                     /locus_tag="VQ514_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HET2500304.1"
                     /translation="MKQSFKTNKLYYGFPVFILGYQDQNFGYNITTCSSSYSLGDWVV
                     IGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEKLERLGVHYEISERTQ
                     TPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVAPELLDEKGHFKNQLFAPTYFM
                     GDGYQRVYRYLDKRVDMKGSFIKKARKKDGKN"
     gene            complement(6643..7038)
                     /locus_tag="VQ514_001561"
     CDS             complement(6643..7038)
                     /locus_tag="VQ514_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HET2500305.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            complement(7134..7388)
                     /locus_tag="VQ514_001562"
     CDS             complement(7134..7388)
                     /locus_tag="VQ514_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HET2500306.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKVGKGFHDLPEGLFGERNY"
     gene            complement(7397..8731)
                     /locus_tag="VQ514_001563"
     CDS             complement(7397..8731)
                     /locus_tag="VQ514_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HET2500307.1"
                     /translation="MTDEWIHILRDILLELHNGASPESVQDRFDATFTGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVLDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYELMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTVKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKEDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKTIMKRLREGSKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            complement(8731..8964)
                     /locus_tag="VQ514_001564"
     CDS             complement(8731..8964)
                     /locus_tag="VQ514_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HET2500308.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLTGTPMDKIVRTLEANGYEVIGLD"
BASE COUNT         2539 a         2015 c         1702 g         2723 t
ORIGIN      
        1 tgattactac aacaataaac gaattaaggt aaaactaaaa ggacttagcc ttgtgcaata
       61 cagaactaaa tccttcggat aaattatttg tctaactttt tggggtcagt acacctaaaa
      121 ctttgatgat atacgttttc ttgtgagaat atttacttca tttttgccta aaattcaatg
      181 tttactcagt atttggatta tgaaaaatcg aggtctaaat ctagatacat tttttctgaa
      241 gacaaatcat tttgaccacc gagcaagaga ttttcaaaaa aagctgttaa aaactcagaa
      301 cgtcgctgta aaatctttgc attatctaat accaaggcat cacgaaaata tttggaatgt
      361 tgctgaaatg gtgtattatc aatatcaaaa ccaaactcac gaagatactg aatcaaaaag
      421 accgttactg tccgagtgtt tccttcgcga aatggatgaa tctgccagat tcctgaaata
      481 aaatgctgga tttgtttaac cacatccgcc tgagttagtg tcgcatatgc aacttgtttt
      541 tcctgattaa aatcataatc taaggtcatt tgaatcatgg agtaatcaga gtacacaaca
      601 ctttcaccat tcaaaacagg ttcattcttt gtgatattgg tctgacgaaa ttgccccacc
      661 ggaatagagg gttcaaatat atcttgaaac aactccttat gaatagcaag taaggtcgca
      721 ggactaaagc taaagcctct tcgagacaat agttctacaa tacgtagaga aaccaagtct
      781 gcctcttccg tacttgcatc aatagtatgg tgataagccg ttgcatcctc ataaacctgc
      841 tcataagtca gttctccccg ggactgtttc tcagccaaag attccatata cgctgatggc
      901 actagattgt caactttctg cagaccaaaa cctatccgcc ataaatcacg cttcgcttca
      961 taagacaagt ttggattgtc aatgttgtaa gttgattgca tagaaatatc ctttcaattg
     1021 tttttcttca ttaccagtaa atcacgaaat tggttggaaa ctggcttaaa ctataaaaag
     1081 tcctcacaat aggttctatg atatatatat atacttcatg taaataataa aagagttata
     1141 atcagcacta ctgataaatt caataccgat ttcgccatct taaaacttag tcaaacgaat
     1201 accgttgatt tccagttcat taagtcgttg acctgtcaag acttctcgtt ccttcaactg
     1261 gaaaagttaa gggggaacta atacaaatcc tctacgcttc tatattcaat gatttcattt
     1321 ttgataatat tctttttaaa ctcaaaatgt tttaaaggat tatcgattaa cactaacttt
     1381 ggaatatcat ctagatttgt gaccaaggat aaaataaaat ccgattgaaa ctccttgact
     1441 ttttcaaatt cttttgcagt taaacgcaca cgccccttag attttcggat accttttact
     1501 tcggcaaggt aagaatactc ttggacatct acctgaaagt catatccatc accataaaga
     1561 cgggcatcgg ttaactgtcc accttggaat ttttcttctt tatcaaaatg aaggataaaa
     1621 taattctcag cctccattcc cgtttcctgt agacgtttga atttagttcg aactattggt
     1681 ttttcaacga tggttatgcc tgttttcttc ccttcactgg caagtaacat tttaactatt
     1741 tcagcataac tatgaacatc ttcatttcca aacatcatat caattaaatc tttacgaaaa
     1801 cgataaactt cagctttctg ccaccaacct tttcgattat tgtcaaaata aggatcaaat
     1861 aaatccattc tattttttac aactccagtt gtttcagcaa ttcccagaga aaccacatag
     1921 cgataaaact ccgacttgct agagcattga aattctttta taaaaagctt gtcaaacttg
     1981 gcaagaccgt agccaattaa atttaacaat tcataatgag ggtgtttgga catcactttc
     2041 tccatcaata gatattgatt tcattatacc aaaaaacacg gctcatcacc gtgtttctgt
     2101 gtttatttca ccaacttctt catctcatca atacgtgcta cggtcgcatc atacttagct
     2161 tggtaatctg cttgcttgtc gcgttctttt tggacaactt ctggtttagc gttggcgacg
     2221 aagcgttcgt tgctgagttt tttgccaacc atgtcgagtt ctttctgcca tttggcaagt
     2281 tctttttcca gacgtgccaa ttcttcttcg acattcaaga ggtctaccag tggcaagaag
     2341 atttcggctc ctgtgatgac gcttgacata gccagttcag gtgcagggat ggttgatgcg
     2401 atttccaagt gttcaggatt tgtgaaacgt ttgatgtagt tgacattgct gttaaagaag
     2461 gcttccaagt cgctatcgct tgtcttaaca aggatagtga taggcttgct tggagcaacg
     2521 tttacttccg cacgcgcatt acgaacggca cggatcaagt ctttaaggct ttccacacca
     2581 gtgtgagcag caaggtcttc aaaggctggg ttgacagttg ggtattctgc tgtaacgata
     2641 gatccttctg agatttgtcc aaagatttcc tctgtcacaa atggcatgat tgggtgaagg
     2701 agacgaagga tcttgtccaa agtgtatagt agaacagaac gtgtgatgac tttctcttct
     2761 tcgttatcgc tataaaggac ttccttggtc aactcaacat accagttggc aaattcttcc
     2821 cagatgaagt tgtaaaggat atgaccagcc acaccaaact cgaacttatc aaagttttca
     2881 gtaacttttg caatggtttc gttgagattg tggagaatcc agcggtccgt cacattacca
     2941 gcctcacctg ttgcaacttt tgtgacattg tcatgcgcca catccagcgt caaaccttca
     3001 ttgttcatga ggatatagcg agaaatgttc caaattttgt taataaagtt ccatgaagca
     3061 tccattttct cgtaagagaa acgaacgtct tgaccaggag ctgagccgtt tgacaagaac
     3121 caacgaagac tatctgtacc atatttatca ataacatcca ttgggtcaat gccatttcca
     3181 agagatttag acatcttacg tccttcttca tcacgaatta gaccatggat aagagcattt
     3241 ttaaatgggc tctttcctgt aaattcaatt ccttggaaaa tcatgcgtga tacccagaaa
     3301 ggaataatat catagcctgt tacgagtgtt gaatttggat agtagcgttt aaagtcagta
     3361 gtttcggtat taggccagcc catggttgag aagggccaga gggcagagct gaaccaagtg
     3421 tcaaggacat cttcgtcctg tttccagcca tcgcccgctg gggcttcctc gccgacatag
     3481 atttcgcctg cttcattata ccaagcaggg atttggtgac cccaccagag ctgacgggaa
     3541 atcacccagt cgtggacatt ttccatccat tgcaggaagg tgtcgttgaa gcgcggtggg
     3601 taaaattcca ccttgtcatc tgtgtcttgg ttggcaatgg cgttcttggc caattggtcc
     3661 atcttgacga accattgagt agacaagcgt ggctcaacta cgacacctgt ccgttctgag
     3721 tgaccgactg aatggacacg tttttcgatt ttgacaaggg caccgatttc ttccaactta
     3781 gcaacgactg ccttacgagc ttcaaaacga tccatgcctg aaaattcaaa ggcaagctcg
     3841 ttcatagttc cgtcgtcgtt catgacgttg acttgtggca agttatgacg ttggccaacc
     3901 aagaagtcat ttggatcgtg ggcaggtgtg attttcacga caccagtacc aaacttagga
     3961 tctgcgtgct catcaccaac gattgggatt aatttattag caattggaag gatgacattt
     4021 ttaccaatca agtccttgta gcgcgggtct tctgggttga ccgcaaccgc aacgtcccca
     4081 aacatggtct caggacgagt tgtagcaact tcaagggcgc gtgaaccatc ttccagcatg
     4141 taattcatgt gatagaaggc accttcgacg tccttgtgaa taacctcgat atcagaaagg
     4201 gctgtgcgag ctgctgggtc ccagttgatg ataaactcac cacggtagat ccagcctttc
     4261 ttgtaaaggt ccacaaagac cttacgaaca gcttttgaca aaccttcgtc aagagtgaaa
     4321 cgctcacgag agtagtctac agagagcccc atcttgcccc attgttcctt gatagtagtg
     4381 gaatattcgt ctttccattc ccagactttc gtcaagaaag actcacgacc aaggtcatag
     4441 cgggaaatgc cctcaccacg caagcgctcc tctaccttag cctgagtggc aatccctgcg
     4501 tggtccatcc caggaagcca aagggtatca aaaccttgca tgcgtttttg acggatgata
     4561 atatcttgca aagttgtatc ccaagcgtga ccaaggtgaa gtttacctgt aacgtttggt
     4621 ggtggaataa cgattgaata aggcttagcc ttttgatcgc ctgaaggctt gaaaacatca
     4681 gcatcaagcc atttttggta acgaccagcc tcaacctcgg ctggattgta tttagatgaa
     4741 agttctttag acatgtgtgt gtcctttctc tattttgttt attttatttt gaatttgctt
     4801 agcagcttct tctgcagaca aattcgtatt atttatttta aagtagtggt gcaactcatt
     4861 cggttgatat tgggaattta attgaagtgt ttcagcggtc tctaaaattt ctctttcaga
     4921 tacctcaata tgtcgtttta agggtttgtg ctttaatcga ttctccgttc gatttcgacg
     4981 tatgcactct tcaagacttg tttccaactc aacaaaaaga atctcttgat gataatcatc
     5041 caacaaattc tgaatttgct ttaaatacat cagttgatac tgatttgaaa aatcaattac
     5101 atctgttaaa attactgatc gctgatttct tgcacttgct ccaaggaatg aaaaagtgat
     5161 tccacgaaca aattcccaca tttcctctgt atagtcctga tagatttcta gtgcaaaatc
     5221 aatggcttga tggttataaa atagggtagc atccgtcagt cgagataatt cttgaccaat
     5281 ggtcattttt cctgatgctg gagcaccaat gatgaaaaga tgcatcaaat cacctcccac
     5341 tcactcttca gcaaggcata tctcaaatca tcacagcagt tgccttgagc atctttacgg
     5401 tctcttatgc gagcttcgag ggtaaagcca agcttttccg cgactcgttg actttgaagg
     5461 ttatatccaa agcaagttag ttcaatcttg tgaagaccca aatctttaaa ggctaagtca
     5521 atcaaggcac gcgcagcttc tggcacataa cctcgccccc aatagtctgg gtgtaaggta
     5581 tagccgattt ccagcacatc atcttcatgg cgatggttga aatcgacaga gccaacgatc
     5641 ttatccgttc ctttgacgac aatcccgtag cctgctggat aggcgacctc tggcaaacta
     5701 gcatagtcaa agatatcctc agcatccgcc acagtacgga ctcgtaaaac gagacgttct
     5761 gtttcgattt tatctggcag ctcagttctt gccatccttc ttcctcgctt ttttgatgaa
     5821 actgcccttc atatctacac gcttatccag atagcgataa acgcgctgat atccatctcc
     5881 catgaaatag gttggggcaa acagttgatt tttaaaatgt cccttttcat ccaggagttc
     5941 tggggcaaca agtcgctcaa gaatcttggc aaagatgtgg caaataccgt cttcctcaac
     6001 aatcctatct acccgacaat ctaaaacaag tggacaggcg tctaaaatag gagtctgagt
     6061 tcgttcagaa atttcataat gcactcccaa acgttccaat ttctcctgat gactgataaa
     6121 accagcctgc tccatcgcaa gcatagaagt ttcatcagaa atattcacag taaatttttg
     6181 atactgtttg atctgctctg cggcattctc tctcgcaacg actccaatca caacccaatc
     6241 tcctagacta tacgaggaac tacaggtcgt gatgttatag ccaaaattct gatcttgata
     6301 tcctaaaata aaaacaggaa aaccataata tagtttatta gttttaaatg attgttttat
     6361 aacaacctcc tttgactaag acacaaaaga aaagccctgc catctacatg acagggacga
     6421 atgtatttat ccgcggtacc acccaatttc gggccaagcc cgcatcttta gtctttaaaa
     6481 taaaaagaca gaaatatttc attttttcag tcattagcaa ccagttttga cacatttgtg
     6541 gacttctcag cgccgccact ttctgtaaaa tgtgggattc aaaacacctc taatagctct
     6601 attgtagcaa gtttttctcg gaaatgcaag gctcaagaaa aattacttga acttgatgcc
     6661 attttctttc aatttcttga tggtagctgg gccgattcct ttcaaggcaa ggagttcttt
     6721 ttcagtccag tttttgaaat ctgaagcaga cttgattcct tcatcataga aagtcttggc
     6781 acgatcaagt ggaagtccac ccaagtttgc tgcaaaatct tctacagaat tagctacttt
     6841 ttgagcttgc tctttggtag cttctactgc ttgttcaact tttttagaaa cagccttacc
     6901 agctgcgctt gctgactgtt ctgcacgctt cacgactttt tttgtctcat ctacaacctt
     6961 agtcacagta cttgaaaagg cacctgcgcg acggaggcca tttcgtaatt gttttttacg
     7021 attgagtttc tttgacatga taaaatcctt tcaataaaaa atccctgctc tgacaaggat
     7081 ttaacataaa cattctatca agattttagt gaaaaatcaa gaatctttct cgtttaataa
     7141 tttcgctcac caaacaagcc ttctggcaaa tcatggaatc ccttgcctac tttgaagttt
     7201 tcaaacttac caagcaagaa ctgataagca tccaagcgac tctcgtagcg aatcatggca
     7261 tctttggcac gctcgtcctg gtcttcttcg taaacttttt gactttcctt gtgcgccatg
     7321 tcgttaatca tgatttgggt attgacccaa gcttcaaatt ccttcataaa ttcttgttcg
     7381 taactcattt ttctccttat tctaatccac gaaaatagtc cgtatcaatc tcacggtagg
     7441 gctggatatc ctgaacatac tccaacactc cttggaattc tccgtcttca tcgtgtactg
     7501 cagcataggt gatgtggaca aacttacctc gcgactcaga cttgaaccac atttcatact
     7561 tgtctttgct tccctcacga agcctcttca tgatagtttt gaccttgtcc aagtacttag
     7621 gcggatggca gagttcgaca ttgcgcccga cttgagacgg cgtccgtttg aaaatcatct
     7681 catcagctgg cgtattgtca ttgtaatact ggaaaatatc ttctttattg acaaaggtaa
     7741 tctccatagg gagatgattg aggatgagat tggcctgctc gactgaaaga tagccattac
     7801 caaaagcctg ttgactatgg cggtccagca cagcttcctt ttccttaggg gtaaaggtaa
     7861 tggtaaaatg gccttctggc gtatctatga cttgttgaac ttgaccttct gccgtatcta
     7921 gctgtacagg ctcctctgca atcttttcct caataaagct ctgtcgttct ggcacccatt
     7981 tctctgacgg acggatgatg gcatagccat aggcatcgct ctcctccgca atctgaagcc
     8041 agtcatcctg agtaaaagac tcaaggagaa tcatgaggag gatggactct tccttgaaaa
     8101 tcatactttc aaactctgtc gcaaaagctt caaaagcttc ctttacactg ctaattgaca
     8161 cttctggtag tgacttgact gtcgttagag ctgtttgaaa gagttcccta atctgatcat
     8221 ccactcccca cataactttg ggaggtgaat cgtgtccata gcgctccatg ataggaaaga
     8281 agagttcttc cttacgttgg taatggatgt caaattgccc cacaagcccc atctgacgca
     8341 ccaagccctt gcgcatctcc gcaagcattt cctcgtcttc catcaactca taggtatcta
     8401 acaatctacg aatgcgaatc aaagctgcac ggagggccaa attttcttcc ttaaagacac
     8461 gaactgggtg cccagggtgt tcggtatcta aaacttcgac acctttgata gcatttttaa
     8521 aaagattggc atggacatca cagagttcca taacatcttc aaaagtgacg cccgaatccg
     8581 agttcatcag ctcgtgctcc ataagggaaa tctcgatggc tgacacgccc gtaaaggtcg
     8641 catcaaagcg atcttgaacc gactcaggag aggcgccatt gtgcaattct aacaaaatat
     8701 cccgtaggat atgaatccat tcatctgtca ttagtctaat ccaatcactt cgtagccatt
     8761 cgcttccagt gtgcgtacaa tcttgtccat aggagttcct gttagcttag aaccctgttt
     8821 aagtgatact ttacgaccaa ctgtattgcg cattaaggga ttggcaaggg gtttaaaacc
     8881 caactccact agaatttcca agacttctgg atgcttgtcc accacttctg caacaggaat
     8941 tgacacatcg atgatattgt ccatgacgac ctccattgt
//
LOCUS       DAVBCW010000080         8885 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVBCW010000080 DAVBCW010000000
VERSION     DAVBCW010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8885
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.080"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            168..854
                     /locus_tag="VQ514_001565"
     CDS             168..854
                     /locus_tag="VQ514_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HET2500309.1"
                     /translation="METKKIKNLKGQIIVSCQALEGEPLYTPNGGVMPLLAKTAFQAG
                     AKGIRANSVRDISEIKEEVDLPIIGIIKRDYDGFEPFISATMKEIDELVSEGVDILAL
                     DCTNRSRPGYDNITDFIHDIKVKYPNQLLMAAISTFEEGKVAAESGVDFVGTTLSGYT
                     PYSPKKDNPDFELVERLVKELDVPVIAEGRISTPEQARKMLDLGAYAVVVGGAITRPL
                     EIAKKFIEVV"
     gene            855..1787
                     /locus_tag="VQ514_001566"
     CDS             855..1787
                     /locus_tag="VQ514_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2500310.1"
                     /translation="MNQEILKKSKIQLTVEHIQKYWILYLMMIPATVLLILFTYGPMY
                     GIIMAFQDFTVFKGYTGSPFVGLKHFQRLFSDPLFYRLFKNTFMVGVLDFLFFFPAPL
                     IFALILNEVRKVRFKSVVQSISYLPHFIPLVVMVGIIFELFGSYGIINSLLSSLGMEP
                     ISFFTKSEWFLPLYIGSGVWKTIGWGSIIYMGALTNIDSTLYEAADMDGANRWHKMWH
                     VTLPSLRPTVVTLFILNAGGIMQVGFEKVFLMSSPATYEVSDVLSTYVYRQGILNSDF
                     SYSAAVGLFNNIVALLFVLLANKIAKKLGEEGIV"
     gene            1798..2727
                     /locus_tag="VQ514_001567"
     CDS             1798..2727
                     /locus_tag="VQ514_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2500311.1"
                     /translation="MIFSKNTKKSNRDKETFVDYLFIFILIIITLLMLYPLWWVVMTS
                     LSEPVWLSKNVVKLFPLKIDITAYKFIFGDSKLWQAYGNSVLYAALSTIVSVAVCSMY
                     AYPLTIPEIRGKKFWNTLILIPMFFSGGMIPLYIQVANLGLLNTAWALVLPGAVSTYY
                     IILFRTQFKSIPMELRESARIDGASEFRIFWNLVLPLSKVIFMIIALYCIVGSWNSFT
                     PPLLYLNDDTKMPLSIYLRNLVVTNTTDTAELSLSAGNTYYQQIAAQGGGIGMRIAIK
                     MGTIVVSVIPIMMIYPFIQKHFVKGILLGSVKG"
     gene            2741..4180
                     /locus_tag="VQ514_001568"
     CDS             2741..4180
                     /locus_tag="VQ514_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500312.1"
                     /translation="MQNLKKYLLLDSGLVVGYTLENAQLKLNKLKKDTENNPLFGEEY
                     FAENPKLWEVRFDNSYPNVIYDKELKLYRCWYQTFVSDEASEETPLAERGEKEYIVKS
                     SRMTALCYAESKDGVKWEKPNLNLVKFKGSKDNNIVLMHAHGAGIMLDEQELDPKKRY
                     KLVSKLEFSPNLHYMVVVFSEDGIHFDEPIPWKKWNPAADSHNFPFRDPKTGKYAVIT
                     RTWANNVRLSAITFSDDFINWSEPKEILRGDGFEDQIYSMPVISYADIYLGFASMYHE
                     GDTLNENYDKVDLELKFSANLEQWDSVCKGDYLIERGGGVYPQNADFDASCIFTAAPI
                     IEDDKIWFYYMGGNGNHTGFRESSLARGYIKKDQFAYYTNKREGIPAKISTVSFQTYD
                     DEIYILADIPENGYIKVAVGTKYGNAYSDFGQEDISLVQVSDGKYKLEFNKKSILDLG
                     NSPVAFKFEIENAKLYGIEGSIQQHYLKY"
     gene            4249..5826
                     /locus_tag="VQ514_001569"
     CDS             4249..5826
                     /locus_tag="VQ514_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2500313.1"
                     /translation="MVKLNKVLKGSVAFLSASLLLAACGNNANSKPAEKTADGKTKIS
                     FLSTWQINDVRPPKDETLVGQWLEEQLGIDIDLVTIPGEGVSSKVNSLITSNQLPTVL
                     LTTGDKSDIAGINKLGKQGAFLDLSQHMDKLPNYKKYLEKNNALAQIEDDEKHIYAFT
                     KFFTDENIMYTTPILRKDLLVGSEFEDLSKIKTVDDYTKVLKYLTEKQGSPAFIQRNG
                     YEGFMKRVTPLWNLSHRTYYDYESDSYKHPVEQPQLKQFVEWLKELRKDNVLHPDWAV
                     MKDETWEGLLASNKGSFTVDRMNIIGDNNFDKSFDWQPLVYPEINGKRYLQPKTPYIS
                     DSGWVINANAPKEEIDKALEFFNFLYADENQEKLAIGFEGKTFTRENPNTQAGIKWLI
                     QIYGENAGNDKAELLFKHGNQAFTRVQTKLDIEAQPGTFPKVMYDQVDKIKKDLGGFR
                     PDTPVLSFTTEELDVLTKSEAGLNTFFDENIIKFIEGQRDMSEWDSFINEAKSKDLDK
                     VTKVYNDAYKRVKDRMK"
     gene            5886..7346
                     /locus_tag="VQ514_001570"
     CDS             5886..7346
                     /locus_tag="VQ514_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET2500314.1"
                     /translation="MYDVVIIGAGSSGALAAIASGRLGLKTCLIEKGSRIGGVPINTL
                     MGSFANLYFDDSGRVNASKIVGELIERIIKKGGTVFNSMEELMNVNDFYQITIPYQPE
                     VYESVLTDMLIESKVDILLNVEVNNIIYDSKNIKSLEILSNQEVKLIDSKVFIDATGN
                     ATIASASGADVYEKNSSYGCLMRVGGVDISKTIEHIEKTKQWNYIEGYNQWLEEVLAN
                     RKYRATGAVLKDPVSYDHAPMLSKDDHFMNDEKWNYIKERWEKFRFIYTLEVSLFRNE
                     LKKAVESGDFNFEIYHDDKSGVTMNGDGISYGSWGQNVALINVAKAFGFNPKYMEDES
                     HANILSRKYNLTFLSFLKKYVPGFENSFILDQGTRATNRSCRHIDNSSMMDEILDFPM
                     YTFKTMYSYQTPKEITYKSIVGGKLNNLFVIGKGAYQSESFRSQISCMLMGISSAAAA
                     KTIIEDKSNTLNINRDLFKSNLDILFTWEFSNWITD"
     gene            7368..7823
                     /locus_tag="VQ514_001571"
     CDS             7368..7823
                     /locus_tag="VQ514_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HET2500315.1"
                     /translation="MNDTNKNQYFVYEFDTSFVEMSYSQFFEGYCILFSKEPYPSLSD
                     MPMEFRKEFLMEMSILGDAMLEVLNAYRINYDILGNSTPVLHAHLFPRYMWEEEERRK
                     TVVHRYDSSVFSNPEFEFTIEKHGPIIKKLRSAIDRHYREYQQLLDDSN"
     gene            7910..8315
                     /gene="cdd"
                     /locus_tag="VQ514_001572"
                     /pseudo
     CDS             7910..8315
                     /gene="cdd"
                     /locus_tag="VQ514_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221146.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
     gene            8328..8777
                     /locus_tag="VQ514_001573"
     CDS             8328..8777
                     /locus_tag="VQ514_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET2500316.1"
                     /translation="MELVEFKNGHVNTVHEHIAITKVKEFLNNHDILKDKGGIHKIST
                     DFFYNIIDMETTTEENRPWESHKEYYDVHYLLNGEETILYNFLSQMELSEYKVDDDWQ
                     QMNGTALFSIKLKKDMLLLLEPNDAHRTGLLVEEPLNIKKVVFKVKI"
BASE COUNT         3093 a         1153 c         1665 g         2974 t
ORIGIN      
        1 gtgtattgaa tctataacag tacgccttaa atgctaaaat atttctataa attaatttaa
       61 ctttcctgat agggctgttc atatcttatt tcaattcact atataaataa aattgtagta
      121 gatgttattg aattagtaaa aaaaataaaa aaacggaggt tttctgaatg gaaactaaaa
      181 aaattaaaaa tttaaaaggt caaataattg tttcctgcca agcgttagaa ggagagccac
      241 tttatactcc aaatggagga gtgatgccgt tattagcaaa aaccgccttt caagcaggcg
      301 ctaaaggtat tcgtgctaat tctgtccgcg atatttcaga aataaaagaa gaagttgact
      361 taccaattat aggtatcata aaaagagatt acgatggctt tgaacctttt atttcagcaa
      421 ctatgaaaga gattgatgaa ttagttagtg aaggagtaga cattttagct ttggattgta
      481 ctaatcgttc aagaccaggt tatgataata ttactgactt tattcatgat attaaagtca
      541 agtatccaaa ccaattatta atggctgcta tttctacttt tgaagaaggg aaagtagctg
      601 ctgaatcggg agtagatttt gtgggtacga cattatctgg ttatacacca tatagcccta
      661 aaaaagataa tcctgatttt gagttagttg aaagattggt aaaagagctt gacgtaccag
      721 ttattgctga aggaagaata agtactccgg aacaagctag aaaaatgtta gatttaggtg
      781 cttacgcagt ggttgttgga ggagctatca ctcgtccttt ggaaatagcg aagaagttta
      841 ttgaggtagt ttagatgaat caagaaatat taaaaaagag taagattcaa ttgacagttg
      901 aacatattca aaaatattgg attttatatt taatgatgat tccagcaact gttctactaa
      961 ttttatttac gtatggacca atgtacggca ttattatggc ctttcaagac tttactgtct
     1021 tcaagggata tactggaagt ccatttgtcg gtttaaaaca ttttcaaaga ttgttctcag
     1081 atcctttgtt ttatagattg ttcaagaata cgttcatggt aggggtttta gattttttat
     1141 tctttttccc agctccgctt atttttgcac tcatattaaa cgaagtcaga aaagtaagat
     1201 ttaagagtgt agttcaatct attagttatc ttcctcattt tattccccta gtggttatgg
     1261 ttggtattat ttttgaacta tttggttcat atggtattat taacagttta ctttcatcac
     1321 ttggaatgga gccaataagt ttctttacaa aatctgaatg gttcttgcct ctctacattg
     1381 gttcaggagt atggaaaaca ataggttggg gttctatcat ttatatggga gcattaacca
     1441 atatagattc aactttatat gaagcagcag atatggacgg ggctaataga tggcacaaaa
     1501 tgtggcatgt aactctacca agtttaagac ctacagtagt tactttgttt atactaaatg
     1561 ctggtggtat catgcaagta ggatttgaaa aggtattctt gatgtctagt cctgcaacat
     1621 atgaagtatc tgatgtatta tctacttatg tttatagaca aggtatttta aattctgatt
     1681 tcagttactc agctgcagta ggattattta ataacatagt agctttgtta tttgttttat
     1741 tagctaataa gattgctaaa aaacttggcg aagaaggaat tgtataaggg gggtaaaatg
     1801 attttttcaa aaaatacaaa aaagagtaat agagacaaag aaaccttcgt tgattatctc
     1861 tttatattca ttttaataat aataacctta cttatgttgt atccattatg gtgggtagtg
     1921 atgacgtcac tttcagaacc agtttggcta tctaaaaacg ttgttaaatt atttccctta
     1981 aaaatagata ttactgcata taagtttatt tttggggatt ctaaactatg gcaagcatat
     2041 ggtaatagtg tattgtatgc tgcactatct acaattgtta gtgttgcggt atgtagtatg
     2101 tatgcctatc cacttacaat tcctgaaatt aggggtaaaa aattctggaa tactcttatt
     2161 ttgattccaa tgttctttag tggaggaatg atcccgcttt atatacaagt tgcaaacctc
     2221 ggtttattga atactgcttg ggctcttgtt ttacctggtg ctgtttctac atattatata
     2281 attttgttta gaacacagtt taaatcaatc ccaatggagc ttcgtgagtc tgctagaatt
     2341 gatggggcaa gtgaatttag aattttttgg aatttagtat taccattatc aaaagttatt
     2401 ttcatgatta tagcacttta ttgcatagtt ggttcatgga atagttttac tcctccatta
     2461 ctttatttga atgacgacac taagatgcct ttatctattt atttgagaaa tttagtcgtt
     2521 acaaatacaa ctgatactgc tgaattatca ctttctgcag gtaatacata ttaccaacaa
     2581 attgctgctc aaggtggagg tattggtatg cgtattgcta ttaagatggg tactattgtt
     2641 gttagtgtta ttccaattat gatgatttat ccatttattc agaaacattt tgttaaggga
     2701 atcctgctag gttctgtgaa aggttaataa ggtaattatt ttgcaaaatt taaaaaaata
     2761 tcttttatta gatagcggtt tagtagttgg gtatacatta gaaaatgctc aattaaaatt
     2821 aaataagcta aaaaaagata ctgaaaacaa tccactattt ggtgaagagt attttgccga
     2881 aaatcctaaa ttatgggaag ttcgttttga taattcatat ccaaacgtta tctatgataa
     2941 agaattgaaa ttatatagat gttggtatca aacatttgtt agtgatgaag caagcgaaga
     3001 gactccttta gcagaacgtg gagagaagga atacattgtc aagtcatcta gaatgacagc
     3061 actttgctat gctgaaagta aagatggagt aaaatgggaa aaaccaaatc tcaatctagt
     3121 aaaatttaaa ggttcaaaag ataataatat tgttctgatg catgcacacg gtgcaggtat
     3181 catgctagat gaacaggagt tggatcctaa gaaacgatat aaattagttt caaaattaga
     3241 attttctccg aatcttcatt acatggttgt tgttttttca gaagacggta ttcattttga
     3301 cgaacctatt ccatggaaaa aatggaatcc tgcagcagat agtcataatt ttccatttag
     3361 agatccaaaa actggtaaat acgctgttat tacaagaacc tgggcaaata acgtacgtct
     3421 aagtgcaatt actttcagtg atgattttat taattggtca gaacctaaag aaatccttag
     3481 gggagatgga tttgaagatc aaatttattc tatgccagta atttcatacg cggatatcta
     3541 tttaggtttt gcttcaatgt accatgaagg cgacacttta aatgaaaact atgataaagt
     3601 agacttagaa ttgaaattct ctgctaattt ggaacaatgg gatagtgtat gtaagggcga
     3661 ctatctcatt gaaaggggag gtggagtata cccgcagaat gctgattttg atgctagttg
     3721 tattttcacg gctgctccaa taattgaaga tgataagatt tggttctact atatgggtgg
     3781 aaatggtaat cacactggct tccgagaaag ttcattagct agaggatata ttaagaaaga
     3841 ccaatttgct tattatacta ataagagaga aggaattcca gctaaaatat ctacagttag
     3901 cttccaaact tacgatgatg aaatttatat cttagctgat attcctgaaa atggttatat
     3961 taaagttgcg gtaggtacta aatacggtaa tgcttattca gatttcggtc aggaagatat
     4021 ctctcttgtt caagtttccg atggtaagta taagttggaa tttaataaaa aatctatctt
     4081 agatttaggt aattctccag ttgcgttcaa atttgaaatc gagaatgcaa aattgtatgg
     4141 tattgaaggt agtattcagc aacactatct caaatattaa ccagaataat tcgttaacaa
     4201 ataatctcta tattagtgga gatagatata taaaatggag gtcttattat ggtaaagctt
     4261 aataaggtat taaaagggag tgttgcattc ctgagtgcaa gtttattgct agcagcttgt
     4321 ggcaataacg ctaattcaaa gcccgctgaa aaaacagctg atggtaaaac gaaaatttcg
     4381 tttctatcta catggcaaat taatgatgtt cgtccgccta aagatgaaac acttgttggt
     4441 caatggttag aagaacagct aggtattgat attgatcttg taactattcc tggcgaggga
     4501 gtaagttcta aagtaaattc tcttattact agtaaccagt taccgactgt tttgttgaca
     4561 acaggagata agagtgatat cgctggtatc aacaaacttg gtaaacaggg tgcattctta
     4621 gatttgtcac aacacatgga caaactacca aactataaga agtaccttga aaaaaataat
     4681 gcattagctc aaatagaaga tgatgaaaaa catatttatg ctttcacaaa attctttact
     4741 gatgagaata tcatgtatac tacaccaatt ctgagaaaag acttacttgt tggttcagaa
     4801 tttgaagatc tttcaaaaat taaaactgtt gatgactata caaaagtttt gaaatatttg
     4861 actgaaaaac aaggttctcc agcatttatt caacgtaatg gctatgaagg cttcatgaag
     4921 cgagttacac cattgtggaa tttaagccat cgtacatact atgactatga atcagattca
     4981 tataaacatc cagttgaaca acctcaattg aaacaattcg ttgaatggtt gaaagaatta
     5041 cgtaaagata acgtacttca ccctgactgg gcagttatga aagatgaaac ttgggaaggt
     5101 ctattggctt ctaacaaggg ttcattcact gttgaccgta tgaatatcat tggagataat
     5161 aactttgata aatcatttga ttggcagcca cttgtttatc cagaaattaa tggaaaacgt
     5221 tatttgcaac cgaaaacacc gtacatttct gattcaggtt gggtaatcaa tgccaatgca
     5281 ccaaaagaag aaattgataa agcgttagaa ttctttaatt tcttatacgc tgatgaaaat
     5341 caagaaaaac ttgctattgg atttgaaggt aaaacattta cacgtgaaaa ccctaatact
     5401 caagcaggta ttaaatggtt gattcaaatt tacggtgaaa atgctggaaa tgataaagca
     5461 gaactgctgt ttaaacatgg taaccaagca tttactcgcg ttcaaactaa attagatatt
     5521 gaggcacagc caggaacatt ccctaaagtt atgtatgatc aagtagataa aattaagaaa
     5581 gatttaggtg gttttagacc agatactccg gttctttcct ttacaactga agaattagat
     5641 gttttaacta aatctgaagc aggattaaat actttctttg atgaaaatat tattaaattc
     5701 attgaaggac aaagagatat gagtgagtgg gattcattta tcaatgaagc taaatctaaa
     5761 gacttggata aagttacaaa agtatataat gatgcctaca aacgtgtcaa agatagaatg
     5821 aaatagacta aatggtagct ctctggtttt tcagagagct actactgcct gagggggagt
     5881 aagctttgta tgatgtagta attataggtg caggaagttc gggcgcattg gctgcaattg
     5941 cttcggggcg tctagggtta aaaacttgtc taatagagaa gggaagtaga attggtggag
     6001 ttcctattaa tacactaatg gggtcatttg ctaatctata ttttgatgat tctggtagag
     6061 taaatgcaag taaaattgtt ggggaattaa tagaacgtat tatcaaaaaa ggtggtactg
     6121 tctttaactc tatggaagaa ttgatgaatg taaatgattt ttatcagatt acaattccct
     6181 atcaaccaga agtttatgaa tcggttttaa ctgatatgtt gattgagtct aaagttgata
     6241 ttttactaaa tgttgaagtt aataatatta tttatgatag taaaaatatt aaaagtcttg
     6301 aaattttaag taatcaggaa gttaaattga ttgattctaa agtttttatt gatgctacag
     6361 ggaatgcaac aattgcttct gctagtggag ctgatgttta tgaaaaaaat tcttcttacg
     6421 gttgtttaat gagagttgga ggagttgata tctcaaagac tatagaacat attgaaaaaa
     6481 caaagcagtg gaactatata gaaggatata atcagtggtt agaagaagta ttagcaaatc
     6541 gaaagtacag agccactgga gcagttttaa aagatccagt ttcttatgat catgccccta
     6601 tgctttcaaa agacgaccat tttatgaatg atgaaaaatg gaattacatt aaagaaagat
     6661 gggaaaaatt tagatttatt tacactttag aagtatcact atttaggaat gaattaaaaa
     6721 aagctgtaga atctggggat tttaattttg aaatttatca tgatgataaa tctggagtca
     6781 ctatgaatgg cgacggtata agctatggtt catggggtca aaatgtagca ttaattaatg
     6841 ttgcaaaagc atttggattc aatcccaaat atatggaaga tgaatcacat gctaatattc
     6901 tttctagaaa atataattta acatttttat cttttcttaa aaaatatgta ccaggttttg
     6961 aaaattcttt tatccttgat cagggaacta gagctactaa cagatcatgt aggcacattg
     7021 ataattcatc tatgatggac gaaattcttg attttccaat gtatacattt aagactatgt
     7081 atagctatca aactcctaaa gaaataacgt acaaatcaat tgtaggtgga aaattgaata
     7141 atttgtttgt aataggtaaa ggtgcttacc aatccgaaag ttttagatca caaattagtt
     7201 gtatgttaat ggggatatca tcagcagcag cggcaaaaac tattattgaa gataaaagca
     7261 atactctaaa cattaataga gatttgttta aatcgaactt agatatatta tttacatggg
     7321 agttttctaa ttggattact gattaacaaa taataaggga atattaaatg aatgatacta
     7381 ataaaaatca atactttgtt tatgaatttg atactagctt tgtagaaatg tcttattctc
     7441 agttttttga aggatattgt attctttttt caaaagaacc ctatccttct ttatctgata
     7501 tgccaatgga atttagaaaa gaatttttaa tggaaatgag tatattaggt gatgcgatgc
     7561 ttgaagtgtt aaatgcctat agaattaatt atgatatctt aggaaattca acacctgtac
     7621 ttcatgctca tttatttccg agatacatgt gggaagagga agagagaaga aaaactgtag
     7681 ttcatagata tgattcttca gtttttagca atcctgagtt tgaatttact atagaaaagc
     7741 atggaccaat tattaaaaaa ctgaggtctg ctattgatag acattataga gagtatcaac
     7801 aattattaga tgattcaaac tgaactccac attttcgatt agatttaata ccttgtatat
     7861 tacaactagg ttgtggatga tataattttt tgaaaaatat attttaggaa tgacttataa
     7921 agcaattata tcagaggcta ttaaatcaat ggccaaagct tatgctcctt attctgattt
     7981 taaggttgga gctgcgttag taactgaaga taaaaaattt tttctggatg taatatagaa
     8041 aatgctgctt tttctcctac ttgctgtgca gaacgagtat ctttttataa agctgtattt
     8101 aaaggagaac gttcgttttc aagaacatgt attgtaggtg gaaaaaatgg tatcttatca
     8161 tcttttatag caccttgcgg agtgtgtcgc caggtaatga tagaatttgt aatcctgatt
     8221 catttgaagt tatattggct agtgatgttg atacttataa agtatataaa cttaaagaac
     8281 tactaccagt tagttttaat caagaaaatt tataacagga gggaactatg gaattagttg
     8341 aatttaaaaa tggacatgtt aatacggttc atgagcatat cgcaataacg aaagtaaaag
     8401 agtttctaaa taatcatgat atattaaaag ataaaggtgg catacataaa atttctactg
     8461 atttctttta caatataatt gatatggaaa ctacaactga agagaataga ccgtgggaaa
     8521 gtcataagga atattatgat gtccattatc tgttaaatgg agaagaaaca attttatata
     8581 acttcttgtc tcaaatggaa ttgtctgaat ataaagtaga tgatgattgg caacaaatga
     8641 atgggacagc tttatttagt attaaattaa agaaagatat gttacttttg ttggaaccaa
     8701 acgatgcaca tagaacaggc ctattggttg aagaaccact taatattaag aaagttgttt
     8761 ttaaggtaaa aatttaactg aaagtacttt cggggattaa gaattttaaa atatttaaga
     8821 aattgtattc taaagttcta aaattataga tatttaattt aaagcttgtt aaaatagaat
     8881 agtaa
//
LOCUS       DAVBCW010000081         8864 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVBCW010000081 DAVBCW010000000
VERSION     DAVBCW010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8864
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.081"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(135..1103)
                     /locus_tag="VQ514_001574"
     CDS             complement(135..1103)
                     /locus_tag="VQ514_001574"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HET2500317.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(1248..2063)
                     /locus_tag="VQ514_001575"
     CDS             complement(1248..2063)
                     /locus_tag="VQ514_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HET2500318.1"
                     /translation="MKKSSILFIFILLLCIGLQYETIYYTDGSRSGAEYGLMGVSIFL
                     ALFYMIPALYFLFRIGKKWELPKKVLILSLLGGMFLSGWLSSFTNTYIHDLLGVLFPD
                     SPFLNAFESAIVAPLVEEPLKLLPLVFVLALIPVRKLKSLFLLGIASGLGFQMIEDIG
                     YIRTDLPEGFDFTISRILERIISGIASHWTFSGLAVVGVYLLYRAHKEQKVGKKQGLI
                     FLGLALRTHFLFNSPFVELETELPLAIPVVTAIALYGFYHAYCFVEKHNELMT"
     gene            complement(2088..2585)
                     /locus_tag="VQ514_001576"
     CDS             complement(2088..2585)
                     /locus_tag="VQ514_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HET2500319.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            complement(2658..4019)
                     /gene="radA"
                     /locus_tag="VQ514_001577"
     CDS             complement(2658..4019)
                     /gene="radA"
                     /locus_tag="VQ514_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HET2500320.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MSVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(4033..4548)
                     /locus_tag="VQ514_001578"
     CDS             complement(4033..4548)
                     /locus_tag="VQ514_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET2500321.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGFPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(4550..4993)
                     /locus_tag="VQ514_001579"
     CDS             complement(4550..4993)
                     /locus_tag="VQ514_001579"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HET2500322.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            complement(5050..5147)
                     /gene="ffs"
                     /locus_tag="VQ514_001580"
     ncRNA           complement(5050..5147)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VQ514_001580"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5180..5647)
                     /locus_tag="VQ514_001581"
     CDS             complement(5180..5647)
                     /locus_tag="VQ514_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HET2500323.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYDILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            complement(5848..7134)
                     /locus_tag="VQ514_001582"
     CDS             complement(5848..7134)
                     /locus_tag="VQ514_001582"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HET2500324.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGIGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGARNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            complement(7365..7601)
                     /locus_tag="VQ514_001583"
     CDS             complement(7365..7601)
                     /locus_tag="VQ514_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HET2500325.1"
                     /translation="MLQKIYEQMTNFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCRRDFIVYHYRVAYRLYLEKLVMNRGFISC"
     gene            7867..8664
                     /locus_tag="VQ514_001584"
                     /pseudo
     CDS             7867..8664
                     /locus_tag="VQ514_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8699..>8864)
                     /locus_tag="VQ514_001585"
     CDS             complement(8699..>8864)
                     /locus_tag="VQ514_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2500326.1"
                     /translation="LCEEFGHKLLPLPPYSPEYNPIEKTWAYIKKNLKKVLPSCNTFY
                     EALFSCSCFN"
BASE COUNT         2844 a         2112 c         1427 g         2481 t
ORIGIN      
        1 tgatgacgta cagtcatatt tcttctcttt ttattatatc acagttttaa atctagcttt
       61 actagattca ccgctactat ctatttattc ggaaaaaaga cgaaaaaacc tgagaatcat
      121 ctcaggcttg gtcattaaat ttttttctca atatcgaaaa gtggagaaag tggtcgtttt
      181 tcatgaatac gtacgatagc atcccctagg agatgagcga ttgaaatctg ctcaatctta
      241 tcaatcaaac gctcttctgg cagatagatg gtatccaaaa caaccaattt cttaatagct
      301 gatttttgga tattgtccgt agcaggacca gaaagaactg ggtgcgtaca gcttgcatag
      361 acttcaacag caccagcttc cgcaagagca tctgccgcat gacaaatcgt tccagcagta
      421 tcaatcatat catcaatcaa gatacaagtc ttgccttcaa ccttaccgat gatgttcata
      481 acttcactag tattcatctt atcaacgcta cgacgtttat caataatagc gatagatgtt
      541 ttcaaaaatt ctgccaactt acgagcacga gtcacccctc catggtccgg gctgacaacc
      601 acatagtcag aaccaaccat accacgacgc tcaaaataat ctgcaatcag aggagcaccc
      661 atcaaatgat ccacaggaat atcaaagaat ccttgaattt gcgcagcatg caagtcgatg
      721 gtcaataaac gatccactcc agctacttca agcatatttg cgacaagttt tgaagtgatt
      781 ggctcacgcg ctctcgcctt tctatcctga cgtgcatacc catagtaagg catgacaaca
      841 ttgacagatt ctgcactcgc acgcttcaaa gcatctacca taatcaaaat ttcaagcaga
      901 ttgtcattta caggcgaact agttgattgt aggataaaga cgtgtttccc acggattgat
      961 tcttcaatgt tgacctgaat ctctccatct gaaaattggc gaacacttga tttccccaac
     1021 tctatcccaa tctcctgcgc cacacgttct gccaattctt tattagaaga aagggcaaac
     1081 agctttaaat cagaaaaaga catgatttcc tccggtatat atgtatcgct tgtgcttttc
     1141 acaagatttt ccatctacca ttgtagcgct ttttgcacta tttttcaatc aaaaataaaa
     1201 gaagggcacc atatttgtac ccttgcatca ttcttttgaa aaatattcta ggtcatcaac
     1261 tcattgtgtt tctcaacaaa gcaataagca tgataaaaac catagagagc aatagccgta
     1321 accactggaa tcgctaaagg caactctgtt tccaactcca caaaaggaga gttaaacaag
     1381 aagtgagttc tcaaggctaa acctagaaaa ataaggccct gtttcttgcc aaccttctgt
     1441 tctttatggg ctctgtaaag caagtaaaca cctactacag ctagacctga aaaagtccag
     1501 tgagaggcaa ttcctgagat gatacgctct aaaattcgcg aaatagtaaa gtcaaagccc
     1561 tctggcaaat ccgtacgaat ataaccaata tcctcaatca tttggaatcc caaaccggaa
     1621 gcaattccaa gtaaaaacaa agattttaat tttcgcacag gaatcaaagc caaaacaaaa
     1681 acaagtggca ataatttcaa gggttcttct accaaaggag ccacaatagc actttcaaag
     1741 gcatttaaaa atggactatc tgggaaaaga acccccagta aatcatggat ataagtatta
     1801 gtaaaactag acaaccagcc tgaaaggaac atccctccca ataaagacag aatcaaaacc
     1861 ttctttggca attcccattt tttcccaata cggaagagaa aataaagagc cggaatcatg
     1921 taaaagagag ctagaaagat agaaactccc attagtccat attccgcacc tgacctcgaa
     1981 ccgtccgtat agtagatggt ttcatactgt aaaccaatac atagcaataa aataaaaata
     2041 aataaaatac tgcttttctt catacacttt ctttctaaat gaagtattta taattctacg
     2101 actgtcatac ttcctgtatc aacattgtaa atggcaccag agataatgac atcgtctggt
     2161 attaggggag actcgataag cagttgcata tcctcgcgta cactctcttc tatatcttgg
     2221 aagggcaaga agtcctggtc tgacacatct acacctaatt cctcttttaa atactcctga
     2281 aaaggttcat tttcaaaggt ctgagcacca cagtctgtat ggtgcaatac cacaatctct
     2341 cttgtcccca tttgttgctg ggaaataact agcgaacgaa tcatgtcttc agtcactcga
     2401 ccacctgcat tccgcaagat atgagcatcc ccaagtgcca agcccagagc ttgcgcaacg
     2461 tgcagacgag agtccataca ggtcacaata gctactcttg ttttgggttt aagtggcaga
     2521 tttaactgcc catgtagggc aacataagcc tgattagctt gcataaactg ttcaaaatac
     2581 gacacgattc cctccttgaa aatttgatag tcaaatattt ctcctatctt atcattttta
     2641 agagaatttg tcacggatta tgcaaagacc tttttcaaaa cttcctgaat cgttgtcacg
     2701 ccaatgacct gaatttcctt aggcagagtg attcctgtca aggaattctt aggtacataa
     2761 atcttagtaa agcccagttt agcagcttcg ttgatgcgtt gctcaatacg attcacgcgc
     2821 cgaatctctc ctgtcaagcc cagttctccg acaaaacatt cctgaggatt agttggcttg
     2881 tctttgtagc tcgaagcaat agcaactgca acagccaagt caatggcagg ttcatccaat
     2941 ttaacaccac cagcagattt gagataggca tcctgatttt gcaagagaag ccctgcccgt
     3001 ttttccaaaa cagacataat caagctagca cggttaaaat caagtcctgt cgtagtacgc
     3061 ttggcatttc caaacatggt cggtgttacc aaagcctgaa cctccgccaa aatcggacgc
     3121 gtcccttcca tggttacaac gatggaggaa ccagtcgccc catccaaacg ctcttctagg
     3181 aaaacttgac tcggattgag tacctcaacc aagccgcccg actgcatctc aaaaatccca
     3241 atctcattag tggaaccaaa acgatttttg accgctctca aaatacgaaa ggtgtggtga
     3301 cgctcccctt caaagtaaag caccgtatcc accatatgct ccaacatacg aggcccagcc
     3361 aaggttcctt ctttggtcac atgacctacg ataaagatgg caatgttatt ggtcttggcc
     3421 aactgcatga gttcagcggt cacttcacgc acctgagaaa cagacccctg cacccctgaa
     3481 atctcaggag acatgatggt ctggatggaa tcaataatga gaaagtctgg ctggatacgc
     3541 tccacttctg cacgaacact ctgcatattg gtctctgcat agagataaaa ctcactatca
     3601 atatcaccta agcgctctgc acgtagttta atctgctggg cagactcctc cccactgaca
     3661 tagagaactg tccccacttg ggacaactgg gttgagactt gtaggagaag agttgatttc
     3721 ccaatcccag gatccccacc gatgaggacg agacttcctg gtaccactcc gcctccaagc
     3781 acacggttga attcctccat ctccgtcttg gttcgattga cattgatgga agtcacctca
     3841 gctagtttca tgggcttggt tttctcacct gtcaaggaca cacgcgcatt cttgacctcg
     3901 gcaacctcaa cctcttccac aaaagaagac caagacccac agttggggca acgtcccaga
     3961 tatttagggg aattataccc acaattttga catacaaatg tcgctttttt ctttgcgatg
     4021 acaaacctct ttctatatct ctaactcaca ctcaatcact tggcaaaaat caatcttctc
     4081 atttggcaca aactggcgca tgagcattcg atgagcaaca actaccacag tctgatgttc
     4141 tcgatactta gacatacatt ctagaaaccg agacttcatt tccgtagctg tctcatattg
     4201 aataggacta ttaggaagca actccccctt gttttctaaa aacagtcttc tagctgtttc
     4261 aaagttttct attcctgttt tatagacctg ccattcatgt aataaaggct ctactcttaa
     4321 aggaaaaccc gtagcacaga ccacatacga agccgtttct aaagctcttg tgactgcaga
     4381 agatacgatt atttcagctg acgagagtaa aggatttttg ctcaatttct ggacttgctg
     4441 ccgtcccatc tcagacaagg gtgccaaatc tatcccaaat cctatataag aacgctcctc
     4501 taactcacgg taatctggct ccccatgacg tacaaagata atcttcattc tagtgccctg
     4561 tcgatccaaa tccaccagtt cgaacgccat cagccgcatc tccatctgca attaagaaag
     4621 tagcaaaaac agcctggaca atacgctccc caacttcaag aacaacctct tggtctgtga
     4681 tattcttcat ctgcgcaaaa atatgccctt catttccagg atttccataa taatccccat
     4741 caatgactcc aactgagtta attaaaacca agcccttctt acgaggattt gaagaacgat
     4801 catagaggta gagaacctca gtcggctgca tataagcctt aacccctgtc ggaaccaaga
     4861 caatctctcc tggcgcaaca actgtacgca cagcaacctt taagtcgtaa ccagccgcat
     4921 gcgctgtctc acgcttgggc aataaatttt catctgtaaa actcgaaacc aattcaaaac
     4981 cacgaatttt cataattttc tcttttctat tatcatttat tctagattat tctatcttat
     5041 ttattcggaa aaagcacgaa aaaaagagca cacaattcaa atcgcttagg gctgctggat
     5101 tcctcccctg acccgcttca cgcagaactg ttgctccact atttattata tcacattcct
     5161 attcatttta aaagcaaaat tattttttcc gtctatttct aaaaaaatcc tgcatgatag
     5221 ctgcgcattc atcttccaaa attcctattt caacctccac acgatgatta agacgctcat
     5281 ctgtcaagat atcgtacaaa cttccagcag cgccaaattt ctggttttta gccccataga
     5341 ccacatttgg aatgcgggca agcccaatcg ccccactaca catgacacaa ggttcaatgg
     5401 tcacaaaaag tgtgcaatcc agcaagcgcc agctctcctc actcaagttc gcatcctcta
     5461 tagccataat ttccgcatgc ataaccgctc gctgtaattc ctcacgcgca ttatgcccac
     5521 gaccaatgat ttccccatct ttgacaatca cacaaccaat tggaatttca tcgtgttcaa
     5581 gagcaatctc agcctctctc aaagcctccc tcataaagac ttctttttct tcaagcgtat
     5641 aatacatctc ttttctcttt tcctacttat cgattttatt attatatcat gaatcccaac
     5701 acaaaaaaag ccaccgaatg cggtgactct atagggagat tattatgaaa aagaaaagtt
     5761 taggatattt gttacaacaa gttaggaggt cttcttgtaa ctgtctatag tatacccgac
     5821 ctatcttaaa caaatcttaa aaatctctta ggaccaaaca ctttctaaaa tatttgtttg
     5881 ttcacgacca ggacctactg agaaagtaga aatacgaacg ccaaccaatt cactcacacg
     5941 acgaacatag ttacgcgcat tctcaggaag atcttccaaa ttgcgagctc cggtaatatc
     6001 ttctgaccaa cctggcaact cttcatagat aggcttgcaa cgtttcaatt gctcaagact
     6061 agctggatag tagtcaatac gttgaccgtc aagatcatag gccacacaaa ttttcacagt
     6121 atccaagccg ctcaaaacat caatagagtt caaagaaagg ttagtaatac cagaaacacg
     6181 acggctatga cgcatcacaa ctgagtcaaa ccaacctaca cgacgtggac gaccagttgt
     6241 tgtaccatat tcatgaccca cttcacggat acgttctccc acttcatcaa acaactcagt
     6301 tgggaaagga ccatctccta cacgactcgt ataagcttta catacaccta caaccttgtc
     6361 aatcttgctt ggaccgatac cagaaccaat tgtcacacca ccagctacag ggtttgatga
     6421 cgtaacaaat ggataagtac cttgatcgat atctagcata acaccttgtg caccttcaaa
     6481 aagcacacgt ttgccattat caagcgcatc attcaagata acagatgtat ctatcacgta
     6541 tttcttgatt tgttgaccat attcgtaata ttcttcaaaa atatcatcga aaacaatcgc
     6601 tttactgtca tacaattttt caaaaagacg attcttttca gcaaggttac gttctaaacg
     6661 ctcacggaaa atatctttat ctaaaagatc tgcaatacga attccaacac gagcagcctt
     6721 gtccatataa gctggaccaa ttcccttaat tgtcgtacca atcttattgt cgcccttagc
     6781 ttcttcttgc aagcgatcca actcgatatg ataaggcaaa ataacatgcg cacgatcaga
     6841 aatacgcaag ttatcagttg ttacaccttc ctcatgaaga tagctcaact cttttacaag
     6901 agatttagga tttacaacca taccattccc aatgacagat attttttcag ggaagaaaat
     6961 cccagatgga atcaagtgca acttaaattt cttaccgtca atcacaatcg tgtgaccagc
     7021 attatcacca ccttggtaac gtgcaattac ttctgcattc gctgaaagga agtctgtaat
     7081 cttcccttta ccttcatcac cccattgggt acctacaaca acaactgaag tcataatttt
     7141 gtctgagccc tcaggctctt cctttctcac atacatggca ggactctcac ctgcaattat
     7201 atcttacaat ttattataat aaaaaatcgc ctttttatca agaagaaaca atagaaagat
     7261 ttgctatttc caactattaa aaaatgattt agaaaaatta ctagctattt actattatct
     7321 ttccataaaa gagtaaatta gttcggaaat ttactaaaat tacctcaaca agaaataaaa
     7381 ccccgattca ttaccaattt ttcaagatac aaacgataag caacacgata atggtaaacg
     7441 ataaaatccc tacgacaacc aatgccatat ctcactaaat aataaattaa aaatttaaaa
     7501 tgaacatgtt cccagtcaaa attatcacca aatgtaggac catactcttc ttcaatacta
     7561 tcatagaaat tagtcatctg ctcataaatt ttttgtaaca taatcaaata ctccttttct
     7621 tttttataaa cttattctaa caaaaaattt tacacattca ctatcaattc ctgaattgtt
     7681 aaaataccag cctcaacaag ataaaaatag gaaaagttga caaaatagaa acaaatttgc
     7741 tacctaaatt tcaaacgatt aaagttctaa aaagaccgcc ttaaaacttt tgggtaaatc
     7801 ctattagaac tataatattt atatatatgt attatggttc tgattttcta gtataaaaaa
     7861 attcaaacta gaattcttct tagaaaaggc cgttttaagt gctatcattg ctcaaaaatg
     7921 gaggtcgctg agacttctat cgtcaagaag aatcatcaaa ttccttgtat catcaaccaa
     7981 aagattgctc agaagttaat tgaaaagact tctatgaccg acattgatca tcagttgtct
     8041 atttcaactt caactgtcat tcgcaagatc aataattttc actttgagca tgatttttcg
     8101 cgtcttcctg agattatgtc ctaggacgtt gaaacagtcc ggggagtgac tgtttcaatc
     8161 gggagatgga gatgagcttt attgctcagg actttgataa actcaatatc ataactgttc
     8221 ttgagagcag aacacaagcc atcatccgaa atcccatgaa tacaaggcta tcaagcgcta
     8281 ctggaagctc attcaacaag atagtcagaa actgagtgat aaacgatttt atcgccctac
     8341 ttttcgtatg cacttgacta ataaagaaat tctagataaa cttttgagct attccgaaga
     8401 cttgaaacac cactataatc tctatcagct cttgcttttc cacctctgtt tcagactttg
     8461 tttaaaacct tcctcaaaga taaagagaaa atcgtcaacg cccttcaatt accttattcc
     8521 aacgccaaac tggaagccac caataatctc atcaaactta tcaagcacaa tgcctttggt
     8581 tttaggaact ttgaaaactt caaaaaagaa aggacgaaat ttgtcctttc tagatcttcg
     8641 ctttcttcaa cccactacag ttgacaaaga accgaaaaat gtctcgcctc taatatagtc
     8701 aattgaaaca agaacaagag aaaagagcct cgtaaaaggt attgcaactt ggtaatacct
     8761 ttttgaggtt ctttttgata taagcccatg ttttctcaat aggattgtac tcaggtgagt
     8821 agggaggaag aggtaaaagt ttatgcccaa actcctcgca taaa
//
LOCUS       DAVBCW010000082         8737 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVBCW010000082 DAVBCW010000000
VERSION     DAVBCW010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8737
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.082"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(314..433)
                     /locus_tag="VQ514_001586"
     CDS             complement(314..433)
                     /locus_tag="VQ514_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HET2500327.1"
                     /translation="MDFKSFIIGLVVGIFGPYMYNLIRKIFSKFPKKDTDSTP"
     gene            complement(436..666)
                     /gene="blpU"
                     /locus_tag="VQ514_001587"
     CDS             complement(436..666)
                     /gene="blpU"
                     /locus_tag="VQ514_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HET2500328.1"
                     /translation="MDTKMMSQFSVMDTEMLACVEGGGCNWGDFAKAGVGGAAARGLQ
                     LGIKTGTWQGAATGAAGGAILGGVAYAATCWW"
     gene            complement(735..1004)
                     /gene="blpJ"
                     /locus_tag="VQ514_001588"
     CDS             complement(735..1004)
                     /gene="blpJ"
                     /locus_tag="VQ514_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpJ"
                     /protein_id="HET2500329.1"
                     /translation="MNTKMLSQLEVMDTEMLAKVEGGYSSTDCQNALITGVTTGIITG
                     GTGAGLATLGVAGLAGAFVGAHIGAIGGGLTCLGGMVGDKLGLSW"
     gene            complement(1471..1668)
                     /gene="blpI"
                     /locus_tag="VQ514_001589"
     CDS             complement(1471..1668)
                     /gene="blpI"
                     /locus_tag="VQ514_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpI"
                     /protein_id="HET2500330.1"
                     /translation="MNTKMMEQFSVMDNEELEIVSGGRGNLGSAIGGCIGAVLLAAAT
                     GPITGGAATLICVGSGIMSSL"
     gene            1950..4103
                     /gene="blpA"
                     /locus_tag="VQ514_001590"
     CDS             1950..4103
                     /gene="blpA"
                     /locus_tag="VQ514_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_125458604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter BlpA"
                     /protein_id="HET2500331.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLVIGDPDPSVKITKMSKERFFSEWTGVAIFLAPKPSYQPHKDKKNGLLSFLPL
                     IFKQKSLIAYIVLSSLLVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVITYIL
                     QQVMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDANSII
                     DALASTILSLFLDVSILILVGGVLLAQNPNLFLLSLISIPIYMFIIFSFMKPFEKMNH
                     DVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYSILQT
                     SLKQGTKLVLNILILWFGAQLVMSSKISIGQLITFNTLLSYFTTPMENIINLQTKLQS
                     AKVANNRLNEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDTLTDINLTIK
                     QGDKVSLVGVSGSGKTTLAKMIVNFFEPYKGHISINHQDIKNIDKKVLRRHINYLPQQ
                     AYIFNGSILENLTLGGNHMISQEDILRACELAEIRQDIERMPMGYQTQLSDGAGLSGG
                     QKQRIALARALLTKSPVLILDEATSGLDVLTEKKVIDNLMSLTDKTILFVAHRLSIAE
                     RTNRVIVLDQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            4114..5475
                     /locus_tag="VQ514_001591"
     CDS             4114..5475
                     /locus_tag="VQ514_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HET2500332.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTN
                     TEVSNQANLSNSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRY
                     LVASQGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLNQATQRLENNTLTSPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
     gene            5532..5660
                     /gene="blpC"
                     /locus_tag="VQ514_001592"
     CDS             5532..5660
                     /gene="blpC"
                     /locus_tag="VQ514_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HET2500333.1"
                     /translation="MDKKQNLTSFQELTTTELNQITGGGLWEDLLYNINRYAHYIT"
     gene            complement(5731..7071)
                     /locus_tag="VQ514_001593"
     CDS             complement(5731..7071)
                     /locus_tag="VQ514_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2500334.1"
                     /translation="MNVAWILLYTLVTHGLKIVIFFKVDGISLTFERIFKAFLFKILL
                     AVVFGMLGYMVGNTYLSYFMEPLYGIGLSFLLLRELPKKLLLFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDDYSFIWLCIIIFNFFISLAFLKWLDYDFTSLRKGILDKDFQK
                     SLTQINWIMGAYYLVIQNLSYFEYEQGIQSTTVRHLILVFYLLFFMGIIKKLDTYLKD
                     KLHERLNQEQDLRYREMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EIYDSVLKDSSEKLQDNKYDLGRLVNIRDKALKSLLAGKFLKARDKNIVFNVEVPEEI
                     QVEGMSLLDFLTIVSILCDNAIEASVEGSQPHVSIAFLKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQNQVFRQVLTVIHTE"
     gene            complement(7085..7822)
                     /locus_tag="VQ514_001594"
     CDS             complement(7085..7822)
                     /locus_tag="VQ514_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2500335.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKEHHITPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANGQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSTRPHRVILYTKTDRLEFTASLEEVLKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINNLH"
     gene            complement(7827..8165)
                     /locus_tag="VQ514_001595"
     CDS             complement(7827..8165)
                     /locus_tag="VQ514_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2500336.1"
                     /translation="MKHMIIQTQKTVYKVNVDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LQNLSNLENQYGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            8442..>8737
                     /locus_tag="VQ514_001596"
     CDS             8442..>8737
                     /locus_tag="VQ514_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HET2500337.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKT"
BASE COUNT         2881 a         1821 c         1357 g         2678 t
ORIGIN      
        1 tatttttttc ttcataagat ttcctcctaa atgtgcgatt tatcttagtt gagcaagaac
       61 atttacactg ctagtatagc acttattttg accttggatc actcaaatca taaatggtca
      121 tcgaaacatc ttgaattgtt aaaaaattta aaaagtaagc attaaaaaca tactttcctc
      181 tttatattgt attgatacca acttgtttgt agacttttca tcctgctatc acatatcatt
      241 ttgacaggcg aaacaaaatt aaagaaactc ccctgtaaat taagctagca aatacagggg
      301 agaaatttat tttttagggg gtactatctg tatccttttt gggaaatttt gaaaatattt
      361 ttctaattaa attatacata taaggaccaa atataccaac tactaaacca ataataaaac
      421 ttttaaaatc cataattacc accaacatgt tgctgcatag gctacacctc caagtatagc
      481 tccacccgca gcaccagttg ctgcaccttg ccatgttcct gttttaatgc ctagttgaag
      541 acctcttgct gctgctcctc caacacctgc tttggcaaaa tctccccaat tgcatccgcc
      601 accttcaacg caagcaagca tttcagtatc cataacagaa aattgtgaca tcatttttgt
      661 atccatgaca aatactcctt ttttaaaaaa ctaaaataaa tcagaataga atcctcataa
      721 ttttactata agtcttacca acttagtccc aatttatcac caaccatacc tcctaagcat
      781 gttaatccac ccccaattgc accaatgtgt gctccaacaa atgcaccagc aagtccagct
      841 actcctaaag tggccaaacc tgctccagtt ccaccagtta taattcccgt agtgactcct
      901 gtaatcagtg cattttgaca atcagtggag ctataccccc cttcaacttt cgcaagcatt
      961 tcagtatcca taacctctaa ctgtgacaac atttttgtat tcatgatgaa tacctccttt
     1021 ttattttcaa tttgttacca aagtcttaaa ttcaataaac aaatagattt tttatagtat
     1081 ctttttgatt ttcttaaaaa agtatatacg tctactatct tcttaaaggt agcagtacct
     1141 attttttagt ctaagatttc aataatcttg agtatctaaa atatcttaat ttcgttattc
     1201 tccttgcaat aaaaagtttt actatactat ttattaactt gcagaaagca aaaaatatta
     1261 gtaaataata gtttatagtt aagtttttta ttcctaccaa tccatcaact aagtaaaaca
     1321 tcaacgatta cataaacgat tgataatata attaaaattt tgctaactat cttattctca
     1381 tcattcttag ataactttga tattttgtaa gtaagtaaat aagacagtaa attaatagcg
     1441 ataataatac tatatttaag aatcataatc ttacaaagag gacataattc ctgaacctac
     1501 acaaataagt gttgctgctc ccccagttat cggaccagtc gcagcagcta atagtactgc
     1561 tccaatacaa ccaccgattg cagatcctaa attgcctctt cctccactaa ctatttcgag
     1621 ttcttcatta tccataacag aaaattgttc catcattttt gtattcatga caaatactcc
     1681 ttttttcttt ttttattttt gtcttgttgt aactttgata agtttagtat atcatcgttt
     1741 tttaaaattt ttcatccaga tcttgaattg tcatcgaaac gtcttgaatt agctttttta
     1801 tttcaagcca cctctaaatg tttaaaaaaa ataatttcta atcacttttt taccattcag
     1861 gaagttttaa tgactattca agatttcata aaatatgaac ttagttttat gacataatag
     1921 acctatccac tatatgaaaa gaattgccaa tgacttctta taaacgtaca tttgttcctc
     1981 aaatagatgc gagagactgt ggtgtcgctg ccttagcctc gattgctaaa ttctatggtt
     2041 cagatttttc tctagctcac ttgagagaac ttgcaaagac caataaagaa gggacgactg
     2101 ctcttggcat tgtaaaagcc gctgatgaaa tgggctttga aacaaggcct gttcaagcag
     2161 ataaaactct ctttgacatg agtgatgtcc cctatccatt tatcgttcac gttaacaaag
     2221 aaggaaaact ccaacattac tatgttgtct atcaaacaaa gaaagactat ctggttattg
     2281 gtgatcctga cccttctgta aaaatcacta aaatgtcaaa agaacgcttt ttctctgaat
     2341 ggactggagt agctattttt ctagctccca aacccagcta tcaaccccat aaagataaaa
     2401 agaatggtct actaagcttc cttcctctga ttttcaaaca aaaatctctc attgcttaca
     2461 ttgttctctc aagcttattg gtcactatta tcaatatagg tggttcttat tatctccaag
     2521 gaatcttaga tgaatacatc ccaaatcaga tgaaatcaac tttaggaatc atctcagttg
     2581 gtctggttat cacctatatc ctccaacaag tcatgagctt ctccagagat tatctcctaa
     2641 ccgttctgag ccagagatta agcattgatg tgattttatc ctatattcgc catatttttg
     2701 aacttcccat gtctttcttt gcgacacgtc gtacaggaga aatcatttca cggttcacag
     2761 atgctaactc tattatagat gccttggctt ctaccattct ttctcttttc ctggatgttt
     2821 ctattctgat tcttgtaggg ggcgtcttac tggcacaaaa ccctaatctc ttccttcttt
     2881 ctcttatttc tattcctata tacatgttca tcatcttttc ttttatgaag cctttcgaaa
     2941 aaatgaacca tgatgtcatg caaagtaatt ctatggttag ctctgccatt atcgaagata
     3001 tcaacgggat tgaaactata aagtcgctca cgagtgaaga aaatcgctat caaaatatag
     3061 acagcgaatt tgtagattat ttggaaaaat cctttaagct cagtaaatat tctattttac
     3121 aaacaagttt aaagcaggga acaaaattag ttctgaatat ccttatccta tggtttggcg
     3181 ctcaattagt catgtcgagt aaaatttcta tcggtcagct gattaccttt aacacacttc
     3241 tttcttactt tacaactcct atggaaaata ttatcaacct ccaaaccaaa ctccaatctg
     3301 cgaaggttgc taataaccgt ttgaacgaag tctatctagt cgaatctgaa tttcaagttc
     3361 aagaaaaccc tgttcattca cattttttga tgggcgatat tgaatttgat gacctttctt
     3421 ataagtatgg ttttggacga gataccttaa cagatattaa tctcacgatt aaacaaggag
     3481 ataaggttag cctagttgga gttagtggtt ctggtaaaac aactttagcc aaaatgattg
     3541 tcaatttctt tgaaccctac aaaggacata tttccatcaa tcatcaggat attaaaaaca
     3601 ttgataaaaa agtcttgcgc cgtcatatta attacctacc acaacaagcc tatatcttta
     3661 atggctctat cttggaaaac ttaactttgg gcggtaatca tatgattagc caagaagata
     3721 ttctaagagc ttgtgaatta gctgaaattc gtcaagacat tgaaagaatg cctatgggct
     3781 atcaaactca gctctctgat ggagctggtc tatcaggagg acaaaagcaa cgaatagccc
     3841 tcgctcgtgc tcttttaact aaatctcctg ttttaatact agacgaagct actagcggtc
     3901 ttgatgtctt gactgagaaa aaggttatag ataatcttat gtctctaact gataaaacca
     3961 ttctctttgt agcccatcgt ctcagtatag ccgaacgaac caaccgtgtc attgttcttg
     4021 accaggggaa aatcattgaa gttggtagtc accaagagtt aatgcaggcg caaggtttct
     4081 accatcatct attcaataaa taaggagaat gtcatgaatc ctaatctttt tagaagcgtc
     4141 gagttttatc agagacgtta ccataactat gcgacagtgt taattatacc tctttcatta
     4201 ctatttactt tcatcttgat tttctccctt gttgccacaa aagaaattac tgttacttcc
     4261 caaggagaaa tcgcccctac aagtgtcatt gcctccattc agtcaaccag tgataatcct
     4321 atcctagcta atcatttagt ggcaaatcaa gtagttgaaa aaggggactt actcatcaaa
     4381 tactctgaaa caatggaaga aagtcagaaa actgccttag caactcaatt acaaagactt
     4441 gagaagcaaa aagaaggact tggaattttg aaacaaagct tagaaaaagc gactgatctt
     4501 ttttctggcg aggatgaatt tggctaccat aataccttta tgaattttac taaacaatcc
     4561 catgatattg aactgggtat cacaaagact aacaccgaag tttcaaatca agctaatctt
     4621 tccaatagca gttcatcagc tattgaacaa gaaattacaa aagttcaaca acaaattgga
     4681 gaatatcaag agttgagaga tgctatcata aataacagag cacgcttacc aactggcaat
     4741 ccgcaccagt caattttgaa tcgttatctt gtagcctcac aaggacaaac acaaggaact
     4801 gcagaggagc catttttatc tcaaattaat caaagtattg caggtcttga atcatctatc
     4861 gcaagcctca aaattcagca agctggtatc ggaagtgtag caacttatga taacagttta
     4921 gcaaccaaaa ttgaagtact ccgcactcag tttttacaga cagcctcaca gcaacaacta
     4981 actgtggaga atcaattaac agaattaaaa gtacaactaa atcaagccac acagcgtttg
     5041 gaaaacaata ccttaacctc cccaagtaaa ggtatcgttc atctgaacag cgaatttgaa
     5101 ggtaaaaata gaattccaac tggtacagaa attgctcaaa tattccctgt catcacagat
     5161 acaagagaag tactaatcac ttactacgta tcttctgact atctacctct actagataaa
     5221 ggacaaactg taagattaaa actggagaag attggaaatc acggcaccac catcatcggc
     5281 caacttcaga caattgatca aactcctacc agaacagagc aaggaaatct ctttaaatta
     5341 accgctcttg caaaactatc taacgaggat agtaaactca tccaatatgg cttacaaggt
     5401 cgcgtcacta gtgtaactac aaagaaaaca tattttgatt atttcaaaga taaaatttta
     5461 acacattctg attaattttc agataacact ctataactat ttattatctt atcaaaaagg
     5521 agaatcataa catggataag aaacaaaacc taacttcatt tcaagaacta acaactaccg
     5581 aactcaacca aattacaggt ggaggattgt gggaagattt attatataac attaatagat
     5641 atgctcatta catcacataa gaacttcatc atccaataca actataaaaa aataagaccg
     5701 agaaacaagt actctcggtc ttatttttca tcattctgta tgtatcacag taagtacctg
     5761 acgaaagact tgattttgac aggtggtatt tagactggta ttaggatggc tttccacaat
     5821 cttcatgacg gtatagagac caactcctct ctcctcccct ttagaactgg ctccaaagga
     5881 gaagatttca gaaatatcga tgccctcttc tttgatggag ttttcgatga taaaggtctc
     5941 ctgtgctcca ttttttaaaa aggcgattga aacatgaggt tgactacctt ccacactggc
     6001 ttcaatagca ttgtcacaaa ggatagacac aatggttaga aaatcaagta gactcatccc
     6061 ctcgacctga atctcctcag gaacttcgac attaaagaca atgttcttat ctctagcttt
     6121 tagaaatttc cctgctagga gacttttgag ggctttatca cgaatattca ccaatcgacc
     6181 aaggtcatat ttattgtcct gcaatttttc actagaatcc tttaagaccg agtcgtagat
     6241 ctcttttatc tgctccatat cctcctcttc aatgcccaga cgtaagctag tcaagaggtt
     6301 ggtgtagtca tggcgaaaac tccgtacttc cttataaagc tcctctatat gccgactata
     6361 ccgctccatc tctctgtagc gcaagtcctg ctcttggttc agtctctcat ggagtttgtc
     6421 cttcaaatag gtatccaatt tcttgataat ccccataaaa aagagcaggt aaaagactag
     6481 gatgagatgg cgaacagtcg ttgattgaat cccctgttca tattcaaagt aagacagatt
     6541 ttgtatcacc aaatagtaag cccccattat ccagttaatc tgagtcaggg acttttgaaa
     6601 atctttatcg agaatcccct ttctcaagct agtgaaatca tagtccaacc atttcaaaaa
     6661 agctagagaa atgaagaaat tgaaaattat tatacataac caaataaatg agtagtcatc
     6721 atatacttgc ccttgtccca aaaatggaag cacaaaatag gaaacacctc tataaaagag
     6781 attcactaat atcattggaa agagaccata aaagagaagg agttttttag gaagttctct
     6841 taacaataag aaagataagc ctatgccgta caagggttcc ataaaataag atagataagt
     6901 atttcctacc atatagccaa gcattccaaa aacaacggcc aacagtatct taaaaagaaa
     6961 ggccttaaaa atcctctcaa aagtgagact aattccatct accttaaaga atatgacaat
     7021 ttttaatccg tgagtaacaa gtgtatacaa caatatccaa gcaacattca taaactctcc
     7081 tagctcagtg taagttatta atagcctcag acacttccct gaccttataa cgcgcgatta
     7141 gacaacttcc accattggga aagaaaagca gtttttcttt cttatctaaa tgcaccacat
     7201 tggcaggatt gatgagaaaa gagcgatggc actgcaagag acgaggctcc tgcttgagaa
     7261 cctcctctag actcgctgta aattccagcc tgtctgtctt ggtatagaga ataacacgat
     7321 ggggtcttgt ggacgtttcg agatagtaaa cctctttaaa aggatactgg aattgagcaa
     7381 attttgattt aaagtaaaag caatcttccg ctagactttt actgtcttga ccattggcat
     7441 agaggagggc tgtctcgata cgagattcaa actcctctgc cgaaagggcc ttatcaatat
     7501 agtccagagc agacacttgg tagcgaaagg acaggggcat aaactccgag tgagtcgtca
     7561 caaagacaat cagggcatac ggatcccgat cccgaatctt tctagccact tccagaccct
     7621 tcatctcctc atttcgaatc tcaatatcca aaaagaatag ctgatgggcc cccttctcat
     7681 gcacctctgc cagcagttgg tctggcttgc caaatacctc aaaagagctg ggagtgatat
     7741 gatgttcctt caagagtttc tcaatcgtcg tttcaatcct agtctgttgg gaaaaatcat
     7801 cctctaaaac aaatattctc atcttcttac tctccctctt tcaaccattt ttgacgaatt
     7861 tctctatagc gacgtctggc atacttgaca gcgtattgac cttcttctcc gagaaaaagg
     7921 attctttctt gaaaatcaat cgattttaat ttatcaagat taaccaaaca atttcgatga
     7981 catctcatca aggtttcccc atattggttc tcaagattac ttaaattttg aagcatatta
     8041 aaactagctt cttctgtaac aatctgtacg gtatgggctt tagttggatg tgtttggata
     8101 tagtagatat cgtcgacgtt tactttatag actgttttct gcgtctgaat aatcatatgt
     8161 ttcattggat tctcctccta agaaaagaat aacagaaaag ttctagccca agcccaccca
     8221 atcctaaaca gtctttgaaa gctcctaaaa ggattatttt tctacaacat catgatgagc
     8281 ttacaactat tttatcataa aattcactca gcgcaattca agacatttca atgacaatta
     8341 aagatttgaa taaaaaaaaa gaattaaaaa tgataaacta acagtatcaa aacacagttg
     8401 ccagaagtaa gactggcacc cagattaaag gaggcattcc catgacagat acagacccca
     8461 tcaaaagagc tcatactttg attactgact taaacaaagc ctaccaagca tgcaaacagg
     8521 caagcgctga cgacgtccgc tttcaggagc aattaaactc tattcttggt tttttagcca
     8581 aggctgaaac agtggataat cgattcttga ttgaactaga aaaattttac cagacttcca
     8641 gtcttctcat gggactcagc gctcttgatc cagatgctcc aactcgcgcc gcttggcggg
     8701 cctatgaccg cttccacttt gaccaagtca agaccaa
//
LOCUS       DAVBCW010000083         8660 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVBCW010000083 DAVBCW010000000
VERSION     DAVBCW010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8660
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.083"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     regulatory      79..167
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            382..1050
                     /locus_tag="VQ514_001597"
     CDS             382..1050
                     /locus_tag="VQ514_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HET2500338.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVDKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     gene            1293..2075
                     /gene="thiM"
                     /locus_tag="VQ514_001598"
     CDS             1293..2075
                     /gene="thiM"
                     /locus_tag="VQ514_001598"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HET2500339.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVMIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            2077..2706
                     /locus_tag="VQ514_001599"
     CDS             2077..2706
                     /locus_tag="VQ514_001599"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET2500340.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFGTQSKDDAGGTIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNMDGVSIISAISYAKNIEKTVREMSEQ"
     regulatory      2915..3003
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3265..3825
                     /locus_tag="VQ514_001600"
     CDS             3265..3825
                     /locus_tag="VQ514_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2500341.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGFVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     gene            3826..5211
                     /locus_tag="VQ514_001601"
     CDS             3826..5211
                     /locus_tag="VQ514_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2500342.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQMI
                     AQVSSLPYSGQVLIDGSEVSQLSIVERVQTVGILLQNPNHQFTMESLFEELVFTMENI
                     GYHLQEIDSKIAEVVQQCRCKDILHRLIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELVYDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMNRVTGELGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGSITWKGSVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            5213..5863
                     /locus_tag="VQ514_001602"
     CDS             5213..5863
                     /locus_tag="VQ514_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HET2500343.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAIFPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQKLHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAYGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            5874..6566
                     /gene="tenA"
                     /locus_tag="VQ514_001603"
     CDS             5874..6566
                     /gene="tenA"
                     /locus_tag="VQ514_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HET2500344.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEINDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            6571..7095
                     /gene="thiW"
                     /locus_tag="VQ514_001604"
     CDS             6571..7095
                     /gene="thiW"
                     /locus_tag="VQ514_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HET2500345.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFAERID"
     gene            7095..7898
                     /locus_tag="VQ514_001605"
     CDS             7095..7898
                     /locus_tag="VQ514_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HET2500346.1"
                     /translation="MQALTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRMAASYANQSSLPMVVDAVGVTTSSIRKS
                     LVKDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPG
                     MSFLVTGPKDLIVSENQVAVLENGCTELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            7891..8523
                     /locus_tag="VQ514_001606"
     CDS             7891..8523
                     /locus_tag="VQ514_001606"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET2500347.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARKVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPV
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
BASE COUNT         2547 a         1522 c         1891 g         2700 t
ORIGIN      
        1 tatatataaa gatatttttc ggtcaaatat cttgctaggg ctttcatttt ttgctatact
       61 ggttcagtaa ttaaataata gagacatttg gggtgcttta ggctgagata atacccattg
      121 aacctgatac agttaagact ggcgaaggga aatgtgaaac gttttttcgt atggcgaaat
      181 gaacgatcta atcttgtaag tcaactctaa ttcttgattg taattggagt tttttgttta
      241 tataaactgg ataggcttgt ttagttagct ctctgcaaat gctctcgatg tgctttaaaa
      301 ggatcaagtg gaattacgat tttgcttgat atttcttggt gttttattaa ttactaacaa
      361 gtaaagagag gagaagtaaa tatggaaaca caagactatg catttcagcc aggtttgact
      421 gttggagaat tattaaaaag cagtcagaag gattggcagg ctgcaatcaa tcatcgtttt
      481 gttaaggaac tttttgcggg gacaattgag aataaggtct taaaggacta tctgattcaa
      541 gattatcact tctttgatgc cttcttatcc atgctgggtg cctgcgtagc tcacgcagat
      601 aaacttgaat ccaagcttcg ttttgccaag caactaggct ttcttgaagc agatgaagat
      661 ggttatttcc aaaaggcttt taaagagtta aaagtagcag agaatgacta tctagaagtg
      721 actttgcacc ctgtaacaaa agcctttcag gatctaatgt attcggcggt ggcttcatca
      781 gactatgccc atcttttggt catgctggtc attgcagaag gtctctattt agactggggt
      841 tctaaagatt tggctttacc tgaagtctat attcattcgg aatggatcaa tctccacaga
      901 ggtcctttct ttgcagagtg ggttcaattt ctggttgacg aactcaatcg tgtcgataaa
      961 aatcgagaag atttgacaga acttcagcaa cgctggaatc aagcagtcgc tttagaatta
     1021 gctttttttg atattggtta cgatgtctag agaaggctca tgatatttac aaatttgatg
     1081 aggtaagact cttcgaaaat ctcttcaaac cacgtcagtt ctatccacaa cctcaaaaca
     1141 gtgttttgag caacctgcgg ttagcctcct agtttgctct ttgattttca ttgagtataa
     1201 atcattcaat gtaaatgaaa aaaattctta acgaaagata gaaataaatc gaaatcagta
     1261 gatcgtataa agtgaaaagg aaggatttca atatgacaag tttaaaatta ttaaaagaaa
     1321 aagcaccatt ggtcatttgt ataaccaatg atgtagtaaa aaatttcaca gcaaatggat
     1381 tagtagcact gggcgcctca ccagccatga gtgagtttcc agcagattta gaggatttgt
     1441 taaagtatgc tggtggttta ttaataaaca taggaacatt gacagatgaa aattggaaat
     1501 tataccaagc tgctctgaaa attgcagaga aatataatgt cccagcagtt ttagatcctg
     1561 tagcctgtgg agcaggagaa tatagaaaaa aagtagcaga tgatctaatc aacaattata
     1621 aactagcagc gattagagga aatgctggcg agattgcctc tttagtagga atagatgtgg
     1681 catctaaagg agtagatagt gcgggcgtag ataatattga cgaaattgct ctagcagcaa
     1741 atgagaagtt caatattcca atagtagtaa caggtgaagt ggatgccatt gctgttaatg
     1801 gagaagtggt aatgattcat aatggtagtg ctatgatgcc gaaagtcatt gggacaggat
     1861 gcttattagg agctgtagta gcaagcttta tcggactaga aaaaggtcaa gaattgaaat
     1921 cattagaaac agcaatgttg gtttacaata tcgctggaga aatggcagaa aaacgtccaa
     1981 atggacatct tcctgggaca tttaaagttg aatttataaa tgccttatac gagattacag
     2041 atgaagatgt aaaggaattc aaaagagtga agtaagatgt ttcataaaga attactaaaa
     2101 ctatatttta tttgtggaac gactacttgc caaggaaaaa atctatatac agtcgttgag
     2161 gaagccttaa aaggtggtat aaccttattt caatttcgag aaaaaggtga gggtgcttta
     2221 gaaggcttag aaaaacttga gctagctatc caaattaaag aactttgtaa aaaatacaat
     2281 gttccattta tcgttaatga cgatatagat ttagcaatgg aaattgacgc cgatggcgta
     2341 catgtaggtc aagatgatat tggtgttgat gaaattagaa aattgatgcc agataaaata
     2401 attggtcttt ctataagaaa cgaagaagaa tttcaacaat caaaagttga atatgtagat
     2461 tatgtaggtg ttgggcctgt atttggtacc cagtcaaagg atgatgctgg tggtactata
     2521 ggttatgaag gtcttgaatt gatgagaaaa ctattgccac aaatgccttt agttgcaatt
     2581 ggtgggatac agacgaaaca tattaaagac attataaaga ccaatatgga tggcgtttca
     2641 atcatttcgg caatatcgta tgcaaaaaat atagaaaaaa ctgttcggga aatgagtgaa
     2701 caatagatat ctatccttgg attctaagta agtaggattt tcctttttta aaaagctatg
     2761 tccagattca gcttgaatgg ctaattcatc aaacaggtca tttgtttttt gcaaaagaaa
     2821 gatctgctgt ctttgtgagt gcaaggctgt atttccagtt caatttcttg aactagtata
     2881 aaatgtgtgt tataataatc acataattaa ataataaaga catttggggt gctttaggct
     2941 gagatgatac ccattgaacc tgatacagtt aagactggcg aagggaaatg tgaaatgttt
     3001 ttttcgtatg tcgaaaagaa ctgtctaatc ttgtaagctg aactctaatt tgtatctgta
     3061 attagagttt tttcttttac gaaactggat gaagatgata tagtagattg aaataagatg
     3121 cgaacaaatc aattagaaaa ttcaaattaa tttctagaaa tattttagtg ttcttgtgta
     3181 ctgttctaaa ttcagtctgc tatacaaagt atttaggtcg gtcttcttga gtgccccact
     3241 tttctttaaa aaaaggagtt ttttatgttg aaaaaatggc agttaaaaga tgttatcttg
     3301 cttgctttct tgtctatctt ttttggtggg gttttcgttg gttcaggata tgtgtataat
     3361 attctcagtc tactcttaac acctcttggt ttgcaggcct ttgccaatga aatcctcttc
     3421 ggtctctggt gtatggctgc gcccattgct gccatctttg ttccgagagt cggaagtgca
     3481 acgattggag aagtgctagc tgcgcttgct gaagtccttt atggtagcca atttggtcta
     3541 ggagctcttt tgtctggctt tgttcaaggt ttgggaagtg aatttggttt tatcgtaact
     3601 aagaatcgct atgaaagttg gctctctcta actgctaata gtattgggat tacgcttgtt
     3661 agctttgtct atgaatacat taagttaggt tactacgcct tttcccttcc gtttgtcctt
     3721 tccttgcttg tggtacgttt tatttctgtt tatttcttct gtaccatctt ggttcgtgcc
     3781 attgtcaaac tctatcatca gtttgcaact ggaggaaaag catagatggg tctggaacta
     3841 cgagcgattc agtccccaat cttctctgag ccgtttgatt ttacttttca tgcgcaagcc
     3901 tttaccttgt tagttgggag tagtgggtct ggaaaatcca gcctctttca aatgattgcc
     3961 caagttagtt ctcttcccta tagcggtcaa gtcctgatag atgggagcga ggtcagtcag
     4021 ctttctatcg tcgaacgtgt ccagacggtt ggtattctct tgcaaaatcc taatcatcaa
     4081 tttaccatgg agagcttgtt tgaggagttg gtttttacca tggaaaatat cggctatcac
     4141 cttcaggaaa ttgattctaa aatagcagag gttgtccagc aatgtcgttg caaggacatc
     4201 ttgcaccgtc tcatccatca cttatcaggt ggggaaaagc aaaaagcagc gctggctgtc
     4261 ctctttgcca tgaatcctag ggtctatctc ttggatgagc ccttcgcttc cattgaccgc
     4321 aagagcagaa tcgagatatt ggagattcta aaagagttgg tctatgatgg gaagacagtt
     4381 attttgtgcg accatgattt atctgactat aaagcctata tcgaccatat ggtggagcta
     4441 agagacggaa aactaaggga agtgtttcaa atcccttcct atgagatgac acaggttgct
     4501 tcaaaggaag ttgcttctag cccggaacta ttccatatga accgtgtgac tggtgagctt
     4561 ggtaatcgcc ccctcttttc aattgctgat ttcacattct atcaagggat ttcctgtatc
     4621 ctgggtgaca atggtgtcgg gaaatcaacc ctctttcggt ctattcttca atttcaaaag
     4681 tataagggga gcattacttg gaagggttcg gtcctgaaaa agaaaaagag tttgtatcgt
     4741 gatctgactg gtgttgttca ggaagctgag aagcagttta tccgagtcag tctgcgagag
     4801 gagcttcaat tagatggacc tgattctgaa agaaatcagc ggatttttca agctttacga
     4861 tattttgatt tggagcaggc agtcgataag agtccctatc aattaagtgg tggtcagcaa
     4921 aaaattcttc agctcctgac catcttgacc agtaaggctt ccgtgatctt gctagatgaa
     4981 ccttttgcag gtttggatga tagagcctgc cattattttt gcaagtggat tgtggaggag
     5041 aggaatcaag gaagaagttt tctgctcatt agtcatcgtt tagacccttt gatttctgtg
     5101 gttgattatt ggattgagat gactagtcag ggtcttcggc atgtgaaaga agtgaccatt
     5161 accaaaccac ttacatctca gagtagcaat acccaagggg aggtgagata gtatggtcaa
     5221 agtagcaacc cagacaccga ttatcagtct cttcttgctg attttatcct tggaaacatc
     5281 tttcattcct tcgattgctc tgaatctttc ggtagtcgca ttttgtattc tctttatgct
     5341 ctattaccgt cgatttaaaa tgttagcttg gatgatcata cttgccattt tcccatcttt
     5401 tgccaactac tgggcagttc agttacacgg agatgcttca caggcagtca tgcttggaac
     5461 gagggccttt gtgacagttt gtatcggcct tgtctttgtt tcctctattt cactaaaaga
     5521 gcttctcttg tacttggctc aaaaggggct atcacgctct tggtcctatg ccttgattgt
     5581 ggtattcaat tcttttcctc tcattcagca agaaatcaag tccctcaaag aagcttgcct
     5641 attacgtggt caaaaactac atttttggtc gcccttgatt tacagtaagg ttctgatgac
     5701 agtctttagg tggcgccatc tttacctgag agctctatct gcttacggat atgacgaaca
     5761 tgcacagttg aagaatagct atcggacttt ttatattcct aaaaaaacaa aattaatcta
     5821 cctgcttttc tttttattgc ttcaaaccag tctattttta taaaggagtt attatggaat
     5881 ttacagatat tgcgatggaa ttatccaaga aagcttggca ggcttccttt catcatccct
     5941 ttattttaca attacaagag gggaatttag aacctgccat tttccgctat tacctgattc
     6001 aggatgccta ctatctgaag gctttctcag aaatctatca tcttttggct gataagactt
     6061 caaaccaaga gatgaaaaga ctcttgaaac aaaatgctca gggtttagtg gagggtgagt
     6121 tatttattcg ccaacaattt ttcaaggaat tggaaatcaa cgaccaggaa atggagcaac
     6181 atccaatcgc tccaacctgt tatcattaca tttctcatat ttataggcaa tttgcagaac
     6241 cgaacttagc catagctttt gcaagcttgc ttccttgtcc ttggttatac catgatatag
     6301 gcaaatcact taatcttaaa ccatcaccaa atcctctcta tcaacaatgg attgaaactt
     6361 atattacgga tgagttagag caacagatca gagaggaggg agcactggtc aatcagcttt
     6421 atcgagaaag tgatgagaca gataagcaaa aaatgctaga tgccttccac atcagtgttc
     6481 atatggaagc caagttttgg gagatggctt accaacacca gacatggaag agcgatttac
     6541 agtctttaga aaaaggagaa gaatagaaac atgagaaagc accaactaca agttcacaaa
     6601 ttgaccattt tatctatgat gattgccctt gatgtagtcc ttacacctat ctttcggatt
     6661 gagggaatgg caccgatgtc cagtgtagtc aatattctag cgggaatcat gatgggacct
     6721 gtttatgcct tggctatggc tacagtgaca gcctttatcc gtatgacgac tcaagggatt
     6781 ccgcctttag ctctcacagg agcgactttt ggagcccttc tagcaggtct cttttataag
     6841 tacggtcgaa aattttactt ttctgccttg ggagaaattg tgggaacagg tattattggt
     6901 tctattgttt cctatcctgt tatggtgctc tttacaggat cggctgctaa gcttagttgg
     6961 tttatctaca ctcctcgatt tttcggagca accttgattg gtacagcgat ttcctttatt
     7021 gcatttagat ttttaatcaa gcaggaattc tttaaaaaag tgcaaggata tttctttgct
     7081 gaaaggatag actgatgcag gcattgacaa atccttttcc tataggctct agttccctca
     7141 ttcactgtat gaccaatgag atttcttgtg agatgctggc aaatgggatt ttggctctgg
     7201 gatgcaaacc tgtcatggca gatgatcccc gtgaagttct tgattttact aagcaaagtc
     7261 aggctctctt catcaatttg gggcatttgt cagctgagaa ggaaaaagca atccgcatgg
     7321 cagcttcgta tgcaaaccaa tcttctctcc cgatggtagt agatgcggtt ggcgtaacga
     7381 cttcatccat tcgtaagagc ttagttaaag accttttaga ctatagacct acggtcctta
     7441 aaggaaacat gtcagaaatt cgaagtcttg ttggattaaa gcaccacggc gttggggtcg
     7501 atgcgagtgc taaagatcaa gaaacggagg atttgcttca agtcttgaaa gactggtgtc
     7561 agacctatcc tggtatgtct ttcttagtta ctggccccaa ggaccttatt gtttcggaga
     7621 atcaagttgc tgtactggag aatggctgta ctgaattaga ctggataaca gggacaggag
     7681 acttggttgg agccttaaca gccgtttttc tcagccaagg aaagactggt tttgaagctt
     7741 cttgcttagc agtctcttat ctcaatatcg ctgctgagaa aatagttgtt caaggaatgg
     7801 gattggaaga atttcgttac caagtactca atcagctttc gctcctaaga agagatgaaa
     7861 attggctaga taccatcaaa ggagaggttt atgaatagag aagcacttag actatatctg
     7921 gtaaccaatc gctatcaaga ttccgtggaa agctttcttg caaaagttga gacggcctgc
     7981 cgttcagggg ttaccatagt ccaattgcga gaaaaaaatc tcacaaccaa tcaatattat
     8041 caactggcaa aacaagtcaa ggaaataaca gatgcctatc aggtcccctt gataatcgat
     8101 gatcggttgg atgtttgtct tgcggttgat gcagcaggtc tgcatatcgg agatgatgaa
     8161 ttaccagttt cggtagctcg caaagtcttg ggtcctgaaa aaatcctcgg tgtcaccgct
     8221 aaaacggtta aaagagctct ggaagcggag aaatcaggtg cagattactt gggtacagga
     8281 gccattttcc caaccaccac caaggaaaat gcgcccatta ctctgatatc gaccttgaaa
     8341 acaatttgcc aaacggttgc cattccagta gttgctattg gaggcttgac ttcggagaat
     8401 attgatcagc ttatgggcac tggcatagct ggtgtagctg ttgtacgtga tttgatgcaa
     8461 gcagaagata ttgaggcaaa aacgcaagcc tttttaacaa agttgcatga cattctttcc
     8521 taattaatac tcaatgaaaa tcaaagagta aactagaaag ctagtcgcag gttgctcaaa
     8581 gtactgcctt gaggttttag ataagactga cgaagtcagc tcaaaacagt gttttgaggt
     8641 tgcagatgga agctgacgta
//
LOCUS       DAVBCW010000084         8480 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVBCW010000084 DAVBCW010000000
VERSION     DAVBCW010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8480
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.084"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            <1..102
                     /locus_tag="VQ514_001607"
     CDS             <1..102
                     /locus_tag="VQ514_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HET2500348.1"
                     /translation="VPQPVQSGDMVRALKEGLINLYKEDGTSVTVRA"
     gene            508..1314
                     /locus_tag="VQ514_001608"
     CDS             508..1314
                     /locus_tag="VQ514_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2500349.1"
                     /translation="MILKDINFALNKGEIVGLVGRNGVGKSTLMKILVQNNQPTSGNI
                     ISSDNVGYLIEEPKLFLSKTGLENLKYLSNLYGVDYNQERFRSLIQELDLTQSINKKV
                     KTYSLGTKQKLALLLTLVTEPDILILDEPTNGLDIESSQIVLAVLKKLALNENVGILI
                     SSHKLEDIEEICERVLFLENGLLTFQKVGKDSHNFLFEIAFSSATDRDIFITKQEFGD
                     IVQEEGLRITMSGNIQSSELFKFFNENSIKVVDFETKKETLKDIYLNRSK"
     gene            1606..2048
                     /locus_tag="VQ514_001609"
                     /pseudo
     CDS             1606..2048
                     /locus_tag="VQ514_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640911.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2438..2647
                     /locus_tag="VQ514_001610"
     CDS             2438..2647
                     /locus_tag="VQ514_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2500350.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            2637..3125
                     /locus_tag="VQ514_001611"
     CDS             2637..3125
                     /locus_tag="VQ514_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500351.1"
                     /translation="MKNRFYYYQLLDEREEQLINKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYVIYLPWVFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            3241..4701
                     /locus_tag="VQ514_001612"
     CDS             3241..4701
                     /locus_tag="VQ514_001612"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HET2500352.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            4698..5522
                     /gene="nadE"
                     /locus_tag="VQ514_001613"
     CDS             4698..5522
                     /gene="nadE"
                     /locus_tag="VQ514_001613"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HET2500353.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            5920..6471
                     /locus_tag="VQ514_001614"
     CDS             5920..6471
                     /locus_tag="VQ514_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2500354.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDIEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGNGMMTETLKAILDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            7171..7878
                     /locus_tag="VQ514_001615"
     CDS             7171..7878
                     /locus_tag="VQ514_001615"
                     /inference="COORDINATES: protein motif:HMM:NF019142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HET2500355.1"
                     /translation="MFRRNLNTSFVHAADGIQYVRDDTRDKEEGIEYDDADNGGIIVK
                     VATKPKVVTKKISSTRIRYEKDETKDRSENPVTIDGEDGYVTTTRTYDVNPETGHVTE
                     QVTVDRKQATDTVIKVPAKSKVEEVLVPCATKYEADNDLSAGQEQEITLGKNGKTVTT
                     ITYDVDGKSGQVTESTLSQKEDSQTRVVKKGTKPQVLVQEIPIETEYLDDPTLDKSQE
                     VEEVGEIGKLLLLQSIL"
     gene            7932..8321
                     /locus_tag="VQ514_001616"
     CDS             7932..8321
                     /locus_tag="VQ514_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HET2500356.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTDWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNASGELAVNTSIDGYRVNDNGEWVR"
BASE COUNT         2760 a         1373 c         1770 g         2577 t
ORIGIN      
        1 gtcccacaac ccgttcaatc aggagacatg gttcgagctc ttaaagaggg gcttatcaat
       61 ctttataagg aagatggaac cagcgtcaca gttcgtgctt aatgtagttg tttagtttta
      121 aaaaattatg caaagctcca tatacaacac ttaaacgaga ttaaagaatg gcgaaatccc
      181 ttgatgcgca agagattagc tgtctttttt attttttaag tgataaagtc ggagcttagg
      241 tatcaaagcc tatcaaatta aacaaagaag cgatgtctta gatattttga aaaaaattaa
      301 taagcagaaa actctctatt attttgttgt agagagtttt ttgttaataa aattttacaa
      361 aatgacattt atatattgca tcaagttaga tatatgatat aatattgtta aaaagaggcg
      421 caacttttta aactcaataa gaatcaaaga gtaaaccatg aatattaatg taggaataaa
      481 aatgtaagta agcattatgg tcattcaatc attctcaaag atataaattt tgcacttaac
      541 aagggtgaaa ttgttggtct agtagggaga aatggagttg gtaagagtac gttgatgaaa
      601 attcttgttc aaaataatca accgacttca ggtaatatta taagcagtga taatgttggg
      661 tatttaatcg aagaaccaaa attattttta tctaaaacag gtttagagaa tttaaaatat
      721 ttgtcaaatt tatatggtgt tgactacaat caagaaagat ttagaagttt gatccaagag
      781 ttagatttga ctcagtctat taataaaaaa gtaaagacct attctttggg tacaaaacaa
      841 aaattagctt tgcttctaac tctcgttacg gaacctgata tattgatttt agatgaaccg
      901 actaatggtt tagatattga atcatcacaa atagttttag cggttctaaa aaaattagct
      961 ttaaatgaaa acgtgggaat tttaatatcg agtcataaat tagaagacat tgaagaaatt
     1021 tgtgagagag ttcttttctt ggagaacggg cttttgacat ttcaaaaagt aggaaaagat
     1081 agtcataatt tcttgtttga gatagctttt tcatcagcta cagatagaga cattttcatt
     1141 accaaacaag aatttgggga tattgttcag gaagagggat tgagaattac tatgtctggg
     1201 aatattcaaa gtagtgagct ttttaaattt tttaacgaaa actctattaa agtagttgat
     1261 tttgaaacta aaaaagagac gcttaaagat atttacctaa atcgttcaaa ataaaggaag
     1321 gttataatca tgaaattaaa taaattgaat tttcttaagg aaaatataag aaatttatat
     1381 tcatcaggcg taatatatct cagtttgctt atctcgttta taccgccgat attgcttaca
     1441 ttctttattc taaagactta agggacatcg cttggtatta agcatatttc aaacttttat
     1501 gctatgctcg gtatgttaat ggctgttata catgctaatc gagtcattag tagagatttt
     1561 tcccacaata cggtaagttt gttttataat caacagaaga atcggatgat ttacgtcttg
     1621 tctaattttc tatatgccat ctcagcttcc attatttatg ctttgaatgg cattgtgtta
     1681 ctagtcatag taagtaaatt gggtattcca ggtgatttag gattagattt tatagtagct
     1741 attgtagtca atacaatttt gttagtcctg ttttattttc tattatctta cattttctat
     1801 ttatacaaat tgaaaagtgg cttggtattt tagtagcttt actactcttt atccctaata
     1861 tattaaatac gatgatgaat actagtaatg atttgtttat caaagcaatt gaacttcttc
     1921 ctttttattc tttacctgta tttgtggctt caaatacgat gtctattagt cagtatcttg
     1981 tggcaatcac tacaatcatt ttattgtact ttttcactct caagaaaagc aagaagtatt
     2041 cattttagtt tcatttagta ttattttgca tacctaaaat acagtaaaaa atcagtcata
     2101 ttggtatgct cctgctttca ctattcaaca cgtttttgac ttatactagg ctcatttcca
     2161 aaagcattat ataatagtga tatgaaacca actaaactaa acaagaaata taagcaataa
     2221 aaactcgttt aaaagatcct actaaagcta atgctaaata aaaataaaaa taaaagagta
     2281 aactaggaag tttatttcaa acaacctaaa atactgattt tcggctgaag ataatactgg
     2341 agtgcaaatt aatggggtta taataaatag ctgacagctt gtgttagttt ggatttttta
     2401 agagtagatg agtattaaaa ctataaggag gacgaaggtg gctaaaaatt taaaattaaa
     2461 attagctcgg gtagagcgtg atttaacaca aggtcaactg gcagaggctg tcggggtgac
     2521 acgccagact attggtttga tagaggcggg aaaatacaat cccagtctct cgctctgcca
     2581 gtctatttgc agatgtttag ggaaaaccct agaccaacta ttttgggagg aagaagatga
     2641 aaaatagatt ttattattat caattactag acgaaagaga agaacaactg atcaataaag
     2701 cgggctctga aagtttctat atctgcatcg ctttgtcgct cctatcttat atcatttcag
     2761 tattagcacc aagccttttt aattctaata tgctgctaat cgttatcatc atagggacat
     2821 tttacttttt taaccgtgcc cgttatctgg gagtgaccta ctatagtcgt tttcatttta
     2881 cgattttggg ttgttttttc ctaaccttgg ctattacggc tcttttgatg ttgcagaatt
     2941 atcaattcaa catagaaatt tatcagcaca atcctttgaa ttttaaatac ctgtctgctt
     3001 gggtcattac ttatgtcatt taccttccgt gggtctttat tggcaatctt ggtcttaaga
     3061 gctatggcga atgggctcag aaaaaatttg aacaagatat ggatgaattg gagagtggag
     3121 aatagcttgt tactcttttc tcaatccagc taaaatgtga tataatagta ctaatttatt
     3181 ggaatacatg aaagttcttg aaaattttca tgggtttcta gctaaggaag taggaaaagt
     3241 atgtatccag atgatagttt gacattgcac acggacttgt accagatcaa catgatgcag
     3301 gtttactttg accaagggat tcacaataag aaggcggtct ttgaggtgta tttccgccaa
     3361 cagcctttta agaacggcta tgcggttttt gcaggtttgg aaagaattgt gaactatctt
     3421 gaagacttgc gtttttcaga tagtgatata gcctatttgg agtcgcttgg ttatcatggg
     3481 gcgttcttgg attaccttcg caatttcaag ttggagttga ccgttcgttc tgcccaagaa
     3541 ggggatttgg tttttgctaa tgaaccgatt gtgcaggtgg aaggacctct agcccaatgt
     3601 cagttggtcg aaacggctct tttgaacatc gtcaactacc agaccttggt ggcgacgaag
     3661 gcagctcgta ttcgttcggt tatcgaagat gaacccttga tggagtttgg gacacgtcgg
     3721 gctcaagaaa tggatgcggc catctgggga acacgcgcag ctgtgattgg tggcgccaat
     3781 ggaaccagca acgtgcgtgc gggtaagctc tttgacattc ctgttttggg aacccatgcc
     3841 catgccttgg tacaggttta tggcaatgac tatgaagctt tcaaggctta cgctgcgacc
     3901 cacaaaaatt gtgtctttct tgtggatacc tatgacaccc ttcgcatcgg tgtaccagct
     3961 gccattcagg tggcgcgtga gctgggtgat cagattaact ttatgggtgt gcggattgac
     4021 tctggggata ttgcctacat ttctaagaaa gtccgtcagc aactggatga ggctggattt
     4081 acagaggcta agatttatgc ttctaatgat ctagatgaaa ataccatcct caatctcaag
     4141 atgcaaaagg ccaagattga tgtctggggc gtgggtacca agctgattac agcttatgac
     4201 cagccagctc ttggtgcagt ttacaagatt gttgcaatcg aagatgaaac tggtcagatg
     4261 cgcaatacga ttaagctgtc taataatgcg gaaaaagtct ctacgccagg taagaagcag
     4321 gtgtggcgca ttaccagtcg tgaaaaaggc aagtcagaag gcgactatat cacttatgat
     4381 ggtgtggata ttagcgacat gacagaaatc aagatgttcc atccgaccta tacatacatc
     4441 aagaagacgg ttcgtaattt tgatgccgtt cctctcttgg tggatatctt caaagaagga
     4501 atattagttt acaacttgcc tagtttgact gacattcagg attatgcccg taaggaattt
     4561 gacaagttgt gggatgagta taagcgtgtg ctcaatccgc agcactatcc agtggatttg
     4621 gcgcgtgatg tatggcaaga taagatggac ttgattgata agatgcgcaa ggaagccctt
     4681 ggtgaaggag aagaagaatg agtttgcaag aaacgattat ccaagagctg ggtgtcaaac
     4741 cagtgattga tgcccaggaa gaaatccgtc gttctattga tttcttaaaa agatatctga
     4801 aaaaacatcc cttcctaaaa acctttgtac tagggatttc tgggggacaa gactcaacct
     4861 tggcagggcg tttggcgcaa ttagctatgg aagaactgcg agctgaaacg ggagacgaca
     4921 gctacaaatt tatcgctgtc cgcctgccat acggagtgca agctgatgaa gcagatgctc
     4981 aaaaagccct agccttcatc cagccagatg tcagcttggt tgtgaatatc aaggaatcag
     5041 ctgatgccat gacagctgca gttgaagcga caggtagtcc tgtttcagac ttcaacaagg
     5101 ggaatatcaa ggcacgttgc cgtatgattg ctcagtatgc ccttgctggt tcccatagcg
     5161 gagcggtcat tggaacagac cacgccgcgg aaaatatcac aggtttcttt accaagtttg
     5221 gtgacggcgg tgcggatatt ctccctcttt accgcctcaa taaacgccaa ggaaaacagc
     5281 tcttgcagaa acttggcgca gagccagccc tttatgaaaa aatcccaacg gcagacctag
     5341 aagaagataa accaggccta gctgacgaag tcgcacttgg agtcacctac gcagagattg
     5401 acgactacct agaaggcaaa acaatcagcc cagaagctca agcgaccatt gaaaactggt
     5461 ggcacaaagg ccaacacaaa cgccacttac ccatcactgt atttgatgac ttttgggagt
     5521 aaaaacctcc attggaggtt tttaccctga gcctagaaat gagaaagcga agaaggtccg
     5581 gggacctttt tagcttcttg ccctgaaatt aaaaagcaag aaaaacctcc actggaggtt
     5641 ttcagcctct catcttgaaa taagaaagtg agagaaggtc tgggggatct tgaaccccga
     5701 gtttagaaat aagaaaatga ggcagattcg gtaactcgaa gagttcgatt tcatcgtctt
     5761 acccctgcaa cgatgactag gtttgaaaaa gcttgctaga gcgcatttca aaccaggcag
     5821 cgactgcgtc aagaaattag aagacaaact cgttttctag ctgttactga gttgagcctt
     5881 tttactacga gcatagaaat aaggaagtga ggtagcatca tgaaatctat cggtacgcaa
     5941 atattacaga cagaacgttt gattttaaga agatttgtgg agagtgatgc agaagccatg
     6001 tttcaaaatt gggcttcatc tgctgagaat ctgacctatg ttacctggga tccccatcct
     6061 gatatcgaaa tcactcgaaa ctcgattcgc aattgggttg cttcctatac taatctcaac
     6121 tattataaat gggccatttg tctaaaagaa aacccagagc aagtgatagg agatatcagc
     6181 atcgttaaga tagacgaggc tgatttaagc tgtgaaattg gctatgtgtt aggcaaggct
     6241 tactggggaa atggtatgat gacagagact ttgaaagcta tcttggactt ttgttttact
     6301 caagcaggtt ttcaaaaggt cagagcacgt tatgccagtc tcaacccagc ttcaggtcgt
     6361 gtcatggaaa aggctggaat gtcctatcta caaaccattg ttaatggtgt agagagaaaa
     6421 ggctatcttg cggatcttat ttattatggt ataagtaggg aagaatgttg aattctattt
     6481 tctgtttcta tcgaagtcaa ctatttattg taaatataat aattagcatt ccaagtttat
     6541 ttgaaacttt aaaatagcat attgattagt acaagacaga tgttctagtt ccttctttaa
     6601 tctggtttag tattagttaa aaaatcgctt taagcttgta actaagaggg agctaatcga
     6661 ctagattctc cagccgaaca ggtggtaatg tactttttat agtgtaatcc tagctgttgt
     6721 taaatttaaa atagaatcct ctatcgagtt agggaattaa attcaaccaa ttttattcat
     6781 gttttttcta tcaaattatc taatattaaa atagtctcat tctgatgaga aaactattct
     6841 atttatactg catagttgta taaggaatga tgaaagatga gtgaaaatta tcaagttgga
     6901 atgtttgtat ctaaatatat tagcatgtat ttagataaga tgtctgcaat cctttatata
     6961 tgagattgat tacgatatag ttaaagtgtt agttcaataa tcaatatatc attctaagtc
     7021 tattttatat ataaagtata ttgtttaacg tttgatagaa aagatgctat actagaagta
     7081 ggatgtaatg gcaaaatcta atactaataa gcgctatgca ttacgtaaac tcggaaagat
     7141 ttttggttct gcggtaatcg ggctaacttt atttttagga ggaatctgaa tacaagtttt
     7201 gttcatgctg ctgatgggat tcaatatgtc agagatgata ctagagataa agaagaggga
     7261 atagagtatg atgacgctga caatgggggt attattgtaa aagtagcgac taaacctaag
     7321 gtagtaacca agaaaatttc aagtacgcga attcgttatg aaaaagatga aacaaaagac
     7381 cgtagtgaaa atcctgttac aattgatgga gaggatggct atgtaactac gacaaggacc
     7441 tacgatgtta atccagagac tggtcatgtt accgaacagg ttactgttga tagaaaacaa
     7501 gccacggata cagttatcaa agttccagct aaaagcaagg ttgaagaagt tcttgttcca
     7561 tgtgctacta aatatgaagc agacaatgac ctttctgcag gacaggagca agagattact
     7621 ctaggaaaga atgggaaaac agttacaacg ataacttatg atgtagatgg aaagagtgga
     7681 caagtaactg agagtacttt aagtcaaaaa gaagactctc aaacaagagt tgttaaaaaa
     7741 ggaaccaagc cccaagttct tgtccaagaa attccaatcg aaacagaata tctcgatgac
     7801 ccaactcttg ataaaagtca agaagtagaa gaagtaggag aaattggtaa attactctta
     7861 ctacaatcta tactgtagat gaacgtgatg gaacaattga agaaactact tctcgtcaaa
     7921 ttactaaaga gatggtaaaa agacgtataa ggagagggac gagagaacct gaaaaagttg
     7981 ttgttcctga gcaatcatct attccttcgt atcctgtatc tgttacatct aaccaaggaa
     8041 cagatgtagc agtagaacca gctaaagcag ttgctccaac aacagactgg aaacaagaaa
     8101 atggtatgtg gtatttttat aatactgatg gttccatggc aacaggttgg gtacaagtta
     8161 atggttcatg gtactacctc aacagcaacg gttctatgaa agtcaatcaa tggttccaag
     8221 ttggtggtaa atggtattat gtaaatgcat cgggtgagtt agcggtcaat acaagtatag
     8281 atggctatag agtcaatgat aatggtgaat gggtgcgtta acagattcct ccctgcgaca
     8341 aaattgataa tagtatgtct gaatatataa ttatatataa ttatatataa aaagctatag
     8401 ataaggtcat cttatagcga ctataaaaaa gtgaacaagc ctagtcttct gtacattaga
     8461 attcagattt gttcactttt
//
LOCUS       DAVBCW010000085         8438 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVBCW010000085 DAVBCW010000000
VERSION     DAVBCW010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8438
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.085"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            72..1349
                     /locus_tag="VQ514_001617"
     CDS             72..1349
                     /locus_tag="VQ514_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2500357.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITSYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
     gene            1362..2009
                     /gene="vex2"
                     /locus_tag="VQ514_001618"
     CDS             1362..2009
                     /gene="vex2"
                     /locus_tag="VQ514_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HET2500358.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQIDYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKDTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            2061..3440
                     /gene="vex3"
                     /locus_tag="VQ514_001619"
     CDS             2061..3440
                     /gene="vex3"
                     /locus_tag="VQ514_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HET2500359.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            3620..3703
                     /gene="pep27"
                     /locus_tag="VQ514_001620"
     CDS             3620..3703
                     /gene="pep27"
                     /locus_tag="VQ514_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198524447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HET2500360.1"
                     /translation="MRKEFHNVLSSGQLLADKRPARDYNRK"
     gene            3752..4408
                     /gene="vncR"
                     /locus_tag="VQ514_001621"
     CDS             3752..4408
                     /gene="vncR"
                     /locus_tag="VQ514_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HET2500361.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASIAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            4405..5733
                     /gene="vncS"
                     /locus_tag="VQ514_001622"
     CDS             4405..5733
                     /gene="vncS"
                     /locus_tag="VQ514_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HET2500362.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGATMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIDFPKVVQD"
     gene            5874..6755
                     /locus_tag="VQ514_001623"
     CDS             5874..6755
                     /locus_tag="VQ514_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HET2500363.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(7076..8266)
                     /locus_tag="VQ514_001624"
     CDS             complement(7076..8266)
                     /locus_tag="VQ514_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HET2500364.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTNNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
BASE COUNT         2609 a         1597 c         1837 g         2395 t
ORIGIN      
        1 attttttttc gtatttcata aacatttcat atttgggttt tataatagtc ttacaaatat
       61 ggaggtgaca aatgaatcca atccaaagat cttgggctta tgtcagcaga aagcgactga
      121 gaagttttat tttatttctg attttattgg tcttattggc cggaatttca gcctgtttga
      181 ctctgatgaa gtccaacaaa acagtagaaa gcaatcttta taaatcactc aatacatctt
      241 tttctattaa gaagatagag aatggtcaga cattcaagtt gtcagaccta gcatctgtaa
      301 gcaagattaa ggggctggaa aatgtctctc ctgaacttga gacggtcgca aaactaaaag
      361 acaaggaagc agtgactggt gagcagagcg tggagcgtga tgatttatca gctgcagaca
      421 ataacttggt tagcttaacg gctcttgagg attcatccaa ggatgtaacc tttaccagtt
      481 cggctttcaa tctaaaagaa gggcgacacc ttcaaaaagg ggattccaag aaaatcctta
      541 tccacgaaga attggctaag aagaacggtc tttcacttca tgacaagatt ggcttggatg
      601 ctggtcagtc tgaatctgga aaaggacaaa cagtagagtt tgagattatc ggcatctttt
      661 ctggtaaaaa acaagagaaa ttcacaggct tgtcttctga cttcagtgaa aatcaagtct
      721 ttacagacta tgaaagtagc caaacccttt tgggcaatag tgaagctcaa gtcagtgcag
      781 cacgcttcta tgtagaaaat cctaaggaaa tggacggact catgaagcag gtagaaaact
      841 tggccttgga aaatcaaggc taccaagtcg aaaaggaaaa caaggctttt gaacaaatca
      901 aagactcagt tgcaactttc caaaccttct tgaccatctt cctttatggg atgttgatag
      961 caggagctgg agccttaatt ctggttttgt ctctctggtt gagagaacgg gtctatgaag
     1021 tggggatttt acttgcactt ggaaaaggca agagctcgat cttcctacaa ttctgtttag
     1081 aggtagtttt ggtatctctt ggagctttgc ttccagcatt tgttgcagga aacggaatca
     1141 caagttatct actccaaact ctactagcaa gtggagatca ggcaagctta caagatacac
     1201 tagccaaagc aagcagttta tcaactagca tcttatcttt tgcagaatcc tatgtttttc
     1261 tagttctgct tagttgctta tctgtagccc tttgtttcct attcttattt agaaaatcac
     1321 cgaaagaaat tttatcatct attagttaag aaggagaaat catgacttta ttacaattac
     1381 aagatgttac ctaccgttat aagaatactg ctgaagcagt cctatatcag atcgattata
     1441 attttgaacc tggaaaattt tacagtatta ttggggagtc aggagcagga aaatccacac
     1501 tcttgtccct acttgctggt ctagatagtc ctgttgaagg ttctatcctt tttcaaggag
     1561 aggatattcg taagaagggc tattcttacc atcgcatgca ccatatttcc ctggtctttc
     1621 aaaattataa cttgatagat tatctttctc cgctggaaaa tatccgcttg gtcaacaaaa
     1681 aggcaagcaa ggatacactt cttgagcttg gtttggatga aagccagatc aagcggaatg
     1741 ttctccagtt atcaggtggt caacagcaac gtgttgccat tgctcgcagt ttggtctcag
     1801 aagctccagt tattctagct gatgagccaa caggaaatct ggatcctaaa actgctggag
     1861 atattgtcga actactcaaa tcacttgccc agaaaacagg taaatgtgtg attgtcgtaa
     1921 ctcacagtaa agaagtggca caagcgtcag atattacact tgaattaaag gataagaaac
     1981 tgactgaaac gcgcaatact agtaaataat ttgagcttat tttgatagaa tgattaaaac
     2041 aaaatctaga aagggaatct atgttacaca acgcatttgc ctatgttaca aggaagtttt
     2101 tcaaatcgat tgtcatcttc ctgattattc tcctcatggc gagcttgagt ttggtcggct
     2161 tgtcaatcaa gggagctact gccaaggctt ctcaggagac ctttaaaaat atcactaata
     2221 gcttctccat gcaaatcaat cgtcgcgtca accaaggaac gcctcgtggt gctgggaata
     2281 tcaagggtga agacatcaaa aaaatcaccg aaaacaaggc catcgagtct tatgtcaaac
     2341 gtatcaacgc tatcggagat ttgactggat atgacctgat tgaaacgcca gaaaccaaga
     2401 agaatctcac tgctgatcgt gccaagcgtt ttggaagtag cttgatgatt acaggtgtca
     2461 atgactcctc taaagaagac aagtttgtct ctggttctta taaactagtc gaaggagagc
     2521 acttaaccaa cgacgacaag gataaaatcc tcttgcacaa ggacttggca gccaaacacg
     2581 gctggaaagt aggggacaag gttaaactgg actctaatat ctacgatgca gataatgaaa
     2641 aaggagccaa ggaaacagtt gaagtgacaa tcaagggact ctttgatggt cataataagt
     2701 cagcagtaac ctactcacaa gaactttacg aaaacacagc tattacagac attcacactg
     2761 ctgcaaaact ttatggatac acagaagaca cagccattta tggggacgca accttctttg
     2821 taacagcaga caagaacttg gatgatgtta tgaaagagtt gaatggcatc agtggtatca
     2881 actggaagag ctacacactc gtcaagagct cctctaacta cccagctctt gagcaatcta
     2941 tctctggtat gtacaagatg gccaacctcc tcttctgggg tagcttgagc ttctcagttc
     3001 tcctccttgc cctcttgctc agcctttgga tcaacgcccg tcgcaaggaa gtgggaattc
     3061 tcctctctat cggcctcaag caggcaagta tcttgggtca attcatcacc gaatctatct
     3121 tgattgctat ccctgctcta gtttctgctt acttcctagc taattacact gcccgtgcaa
     3181 ttggaaacac tgtccttgcc aatgtgactt caggtgttgc caaacaggct agtaaggcgg
     3241 ctcaagcctc taaccttggt ggtggtgcag aagtagatgg ctttagcaag accttgtcga
     3301 gcctagacat ttccattcag acatcagact ttatcatcat ttttgtcctt gccttggttc
     3361 tagtggttct cgttatggcg cttgcttcaa gcaatctcct tagaaaacaa ccaaaagagc
     3421 tcttgctgga tggtgaataa atttgaaaaa atgagtctag aataaagatt gcatcttgtg
     3481 tttctattca agaatagtgg ataggaatgg ctatttaaca attcaaaata aatccgaaag
     3541 cagtggtgaa aatcattgct ttcagttgct ttctttgtac tttagtgctt aaatataata
     3601 tactaaagtt atggaattta tgagaaagga atttcacaac gttttatcta gtggtcagtt
     3661 gcttgcagac aaaaggccag caagagacta taatagaaaa tagagtaggt atttattcta
     3721 agaaaaataa aaaatagaga gcagttaaag tatgaaaatt ttaattgtag aagatgaaga
     3781 gatgatccgt gagggggtca gtgattattt gacggattgt ggctatgaaa ctattgaggc
     3841 agcggacggt caggaagctc tggagcaatt ttctagctat gaggtggccc tggttttact
     3901 ggatatccag atgcccaagc tcaacggctt agaagtccta gctgagattc gtaaaaccag
     3961 tcaggttcct gtcttgatgt tgacagcttt tcaagatgag gaatacaaga tgagtgcctt
     4021 tgcctctttg gcagatggct atctggaaaa acctttctcc ctctcccttt taaaagtgag
     4081 ggtggacgcg attttcaagc gctactacga tacaggacga atcttttctt acaaggatac
     4141 caaggtggac tttgaaagct acagtgcaag catcgcaggt caagaagtgc ctatcaatgc
     4201 caaagagttg gaaattctgg actatctagt gaaaaatgaa ggccgggcct tgactcgatc
     4261 tcagattatc gatgccgtct ggaaagcgac agatgaggtt ccctttgacc gtgttattga
     4321 tgtttatatc aaggaattgc ggaaaaagct agacttggat tgtatcctca ctgtgcgcaa
     4381 tgttggttat aaattggagc gaaaatgaaa cgaacaggtt tatttgcaaa gatatttatc
     4441 tataccttct cgatatttag tgttctggtt atctgccttc atttagctat ttattttctt
     4501 tttccttcga cttatctgag tcatcgtcag gaaaccattg gtcaaaaggc aacagccatt
     4561 gcccagtccc tagaagggaa agataggcag agtatcgagc aagtgttaga cttgtattcc
     4621 cagactagtg atatcaaggg gaccgtcaaa ggtgagatga ccgaggacaa gttagaagtc
     4681 aaggacagtc ttcctctgga cacagaccgc cagacaacct ctctctttat tgaggagcgc
     4741 gaggtgaaaa cgcaagacgg tgctactatg attctccagt ttctagcttc catggattta
     4801 caaaaggaag cggagcaaat cagtctccag tttcttccct ataccttgct ggcctccttt
     4861 ctgatttccc tcttggtggc ctacatctac gctcggacta ttgttgcacc gatcttggaa
     4921 atcaagcggg tgacccgtcg gatgatggac ctggattccc aagtgcgatt gcgcgtggat
     4981 tctaaggatg agataggtaa tctcaaggaa caaatcaata gcctctacca gcatctcttg
     5041 actgttattg cggacttgca tgaaaagaat gaagccattc tccagctgga gaagatgaag
     5101 gtcgaattcc tacgaggagc ttctcatgaa ttgaaaacac cgctggctag tttgaaaatc
     5161 ctaatcgaaa atatgagaga gaatatcggt cgttataagg atagagacca gtatctggga
     5221 gttgccttgg ggattgtgga tgaactcaat caccatgttc tgcagatact ttccctctct
     5281 tctgtgcagg aattgcgaga tgatagggaa acaattgacc tcctccagat gacgcaaaat
     5341 ctggtcaaag attatgcctt gctagccaag gaaagagagc tccagataga caatagtttg
     5401 acccatcagc aggcttatct aaacccatca gttatgaagt tgattctttc taatctcatc
     5461 agcaatgcca ttaagcactc tgttccaggt ggcttagttc gaattggaga aagagaagga
     5521 gaacttttta tcgaaaatag ctgtagctca gaggaacaag aaaaactagc ccagtctttt
     5581 tctgacaatg ccagtcgcaa ggtcaagggg tctggtatgg ggctctttgt ggttaagagt
     5641 ctattagaac atgaaaaatt agcttatcgt ttcgagatgg aggagaatcg tttaaccttc
     5701 tttatagatt ttccaaaagt cgtccaagac tagggagaga aagggtttac atagatggag
     5761 ttagaagaaa atcaatcgaa actgcgggaa aaactagatt tttttggcaa aaagtgataa
     5821 aatgaacaat gtaaatggga tgacccataa aaatatacag gaggcctgat aaaatggcaa
     5881 tcgtttcagc agaaaaattt gtccaagcag cccgtgacaa cggttatgca gttggtggat
     5941 ttaacacaaa caaccttgag tggactcaag ctatcttgcg cgcagcagaa gctaaaaaag
     6001 ctccagtttt gatccaaact tcaatgggtg ctgctaaata catgggtggt tacaaagttg
     6061 ctcgcaactt gatcgctaac cttgttgaat caatgggtat cactgtacca gtagctatcc
     6121 accttgacca cggtcactac gaagatgcac ttgagtgtat cgaagttggt tatacttcaa
     6181 tcatgtttga cggttcacac cttccagttg aagaaaacct taaattggct aaagaagttg
     6241 ttgaaaaagc acacgctaaa ggtatctcag tagaagctga agttggtact atcggtggtg
     6301 aagaagacgg aatcatcggt aaaggtgaat tggctccaat cgaagacgct aaagcaatgg
     6361 ttgaaactgg tatcgacttc ttggcagctg gtatcggtaa catccacggc ccttacccag
     6421 taaactggga aggtcttgac cttgaccact tgcaaaaatt gacagaagct cttccaggat
     6481 tcccaatcgt attgcacggt ggatcaggta ttcctgatga gcaaatccaa gcagctatca
     6541 aacttggtgt tgccaaagtt aacgttaaca cagaatgcca aatcgcattc gctaacgcaa
     6601 ctcgtaaatt tgctcgtgac tacgaagcaa acgaagcaga atacgacaag aaaaaactct
     6661 tcgacccacg taaattcttg gctgacggtg taaaagctat ccaagcatcg gttgaagaac
     6721 gtatcgacgt attcggttca gaaggtaaag cataatctag ctgaataata cagataaaac
     6781 ctgcctgtga gggtgggttt tttgatttca agctactcgt tttagtatag taaactgaat
     6841 ctagactagc acaatgctgc ttctatagcg ttttgaaact agaataggac aacataactg
     6901 ctaaaagatt tctataaatt catttgattt tcctaatcaa tttgttcgta tcctatttca
     6961 ctccactata aaatattctt atcaattgat ttgaatgcca aaattccatc gttccggttt
     7021 tatttcattt ggctataaaa aactctcctg ggaaatccca ggagagtatg attacttatt
     7081 tgaacttgaa tccttcgtaa ataagctctg tttttggatt ttgtttctta atctgtttgg
     7141 caagtgcctt catcatagaa ataggaccac acatatagac ggttgcatgt tcgggcactt
     7201 ctttttgttc aaaattaaga tagccgtctt tcgtactgtc gattagatgg agttcaaaat
     7261 taggattttt ctgagcatag ttacggagta aatctaggta gactgcattt tcatctccac
     7321 ggaagctata gtagaagtga acctgtttat ctaaaatagg atgttcacgg atgtaagaga
     7381 tgaagggggt gatcccaata cctccagcaa tccaaacctg attttctcgt ccttcttcta
     7441 tgatcatgtg tccgtaagct ctgtctaggg ttactttgct gccggcttga agattatcat
     7501 agatattatt ggtatggtcg cctgaatttt taacagtaaa gtaaagagtt tgaccatgac
     7561 ctcctgagat agaaaaggga tgcggagcac tttcaaagcc ttcttggaaa atctttagaa
     7621 aggcaaattg tcctgattga tagttgaaag gtctgctaag atggatttga atttctctag
     7681 tatcgtgatt taagcgtttg agatgggtaa tttttcctag ataggggaag gaaatctttt
     7741 gatatagaaa aatgatataa aaaccagcta gtaagcctaa aagggcatag ctaccaacaa
     7801 gaaaacttag aagattaaat gtaaggagac gattgcccat tatcatgtag atgtgaaaga
     7861 gtcctaaaat ataggctagg taaaccaggc ggtgaatcca tcgccaagct tcgtattgga
     7921 tgtatttgcc taaataggcg acaaggatga tgctggcaaa gatatagatg gcaagattac
     7981 caaactgagc agctaagcga gagccccaca aaccgcccat actaaagtta tgaaagatta
     8041 gtaggatgat ggagagaaag gctgtgaatt tgtggacggt gtagaccttc tccaaaccgt
     8101 gaaaccagct ttctagtagt gggagacgag tggctaggat aaaagtcaga gataggcttg
     8161 ttaaagctag tcctggaatc atgaattggg gagaagtgtt catccaagtc aaaagagtca
     8221 agataaaact agctatgata aagagtagtc ctttgactga tttcatagaa aattccattt
     8281 catttagatt tcgatttgtt gtaaatgaat ttgttacatt ttatcataga aaatgtatgg
     8341 tgtcaaattg aggtctataa atatctactc tcatcaaaaa actctccaat tgaactggag
     8401 agtggctgtt tatacttaat gaaaatcaaa gagcaaac
//
LOCUS       DAVBCW010000086         8195 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVBCW010000086 DAVBCW010000000
VERSION     DAVBCW010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8195
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.086"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(<1..947)
                     /locus_tag="VQ514_001625"
     CDS             complement(<1..947)
                     /locus_tag="VQ514_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2500365.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQVIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFILVTFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTI"
     gene            complement(1027..1890)
                     /locus_tag="VQ514_001626"
     CDS             complement(1027..1890)
                     /locus_tag="VQ514_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HET2500366.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTSLYFELNWILLQGLICQRDASYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQEAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(1893..3794)
                     /locus_tag="VQ514_001627"
     CDS             complement(1893..3794)
                     /locus_tag="VQ514_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET2500367.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFNDLRRTERQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETISWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENAAV
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINCVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSDQV"
     gene            complement(4222..4497)
                     /locus_tag="VQ514_001628"
     CDS             complement(4222..4497)
                     /locus_tag="VQ514_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET2500368.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(4603..5442)
                     /locus_tag="VQ514_001629"
     CDS             complement(4603..5442)
                     /locus_tag="VQ514_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET2500369.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVIVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRIGRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            5584..6432
                     /locus_tag="VQ514_001630"
     CDS             5584..6432
                     /locus_tag="VQ514_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HET2500370.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSGWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(6525..7442)
                     /locus_tag="VQ514_001631"
     CDS             complement(6525..7442)
                     /locus_tag="VQ514_001631"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HET2500371.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            7879..8084
                     /locus_tag="VQ514_001632"
                     /pseudo
     CDS             7879..8084
                     /locus_tag="VQ514_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         2555 a         1714 c         1360 g         2566 t
ORIGIN      
        1 aaaatcgtca taaacaattc acaaactaaa tttccagaaa aggaaagaaa agttggaagt
       61 ggtaatccca tcataaaaac tccgacacct gtcaaagcca gtaaaatcaa aagattataa
      121 atattagctt taattttact agctagaaga gccccaatga tggaaccaat agcccccata
      181 gttaaaatac ttgcataggc tccttctgac ccgtaaagct gattcgaaaa gggaagtaga
      241 aattcaaaag ctgcaaaaaa gaaattaacg ctggaagcta ccagcaaaag gaagaaaatt
      301 tcttgctgat gccagatata gtgtaaccca tccttgatat ctacaaaaat atctctccca
      361 gtaaaagcct ttttctcttg aacttttgct tcctcttttg gaaggaaagt cactagaata
      421 aaagcaatga aaaaagtcag cgagtctagc agtagcgtca tatggagact tgcaaactgt
      481 aaaacaagga aggaaagaac aggagagcta acacctacaa cctgcaaaac cagctctaag
      541 cgagaattat agatcacaat ctcatctttc tccaccactt cagttatgat agctttattg
      601 gctgtgcgag aaaaggcaaa agcaataacc tgcacaatgt tagcaacaat caaagcgcca
      661 atcatccagc tatcattcct tatgaaagaa atagccagac aaagaatccc acaaacaaga
      721 tctgccgtca ttaaaatctt acgacgagaa aaacggtctg aaataactcc gccaaaggga
      781 ttgacgagaa tagatgtgac gagctcagaa atctgataca ttcctaaaac tgtctgtcct
      841 atagtcccca tagaagccaa ccagacacta tttccataat catagagcat atttcccatt
      901 ttattgatag ccccacggct aatcaactgc actgcatagc gattcatatt aaagctcctc
      961 tcaaattttg aaactattgt atcaaaaccg aaaggagctt tttatttttt cccttatttg
     1021 ggaaaattaa cttttgacaa atttttcgta gtgttcctga taataggcta cttgctctgg
     1081 aagacctaac acatcaaaaa tatgcatggc ctcttgcatc tgcttacagc cttctttaca
     1141 ctgtcctttt tgatataagg caaaaccttt taaataatgg aaaacattac gctcataaag
     1201 cttaatacct ttgtcaataa tcttctctgt ataagcctca aaatagttgg cattataaaa
     1261 agaagaatgc tctaaacaat gctggtaaca attgagggcc aaaatcaaca ctaatctctt
     1321 atggcgacta atctcttggt aaaattcctc cctctccata acttctctac caatccgagt
     1381 gacatagtct acatcgtaga aactatagag gttaccgaaa agaatcaact catacatggt
     1441 ccattcttct gttttgaaga gataatctgc taccttatcc aaatcatcct gcttcatatc
     1501 ataactcgca tctctttgac aaatcagacc ttgtagcaaa atccagttca gctcaaaata
     1561 aagggaagtc gtcgaactct tagacttttc aagttgttct ctttgaagct tttgaaaacc
     1621 tgcaatatcg tttgaatagt aaagtgggat aatctgtgcc atcatagaca catgttcatg
     1681 attatgaaaa ttccttgcct tatccatgaa attttcgatt gttacatgaa tgttatccaa
     1741 aatctcaaag aaacgggaga ctgccaggtc agactcccca agctcaaagc gagataactg
     1801 agaggtagag caggattcgc ctgctgcttc ctttaaagaa taatttccac ttgttcgaaa
     1861 ttcacgaaat acttttccaa gatgttccat ctttacacct gatctgataa ttcttcccac
     1921 tcaagcatag cttcttcctg acgatggctg attttatcca gctcagcctg taattccatg
     1981 agtttgtcgg catcgtttgt ttccaacatt tgttcagaaa tggcttggct ttgactttct
     2041 agctcttcaa tttcagcttc tagactttcg atttgtcgca tgagtttgcg aacttctttt
     2101 tgactttctt tctgggcctg atagtcattg actggacttg cttcctttgc ttgattgcta
     2161 gttgaagctt cctcagtctg actcatttct gctgttgctt tcttctcaac atagtagtcg
     2221 taatctccaa ggtagagagt tgaaccattc tcagacaatt ccaaaacatg agttgccaca
     2281 caattgataa agtaacgatc atgactgaca aaaagcaagg taccatcaaa gtcaatcaag
     2341 gcattttcta gcacttcctt actatcaata tccaagtggt tggtcggctc atccagaatc
     2401 aaaaagttat tgttttccat agacaattta gctaaaagca aacgagcttt ttcgccacca
     2461 gatagcatgc cgactgattt tttaacatca tctcctgaga aaaggaaggc tccaagacgg
     2521 ttgcggattt caacttctgg tgtcaatttg aaatcattcc agagttcatc cagcaccgta
     2581 ttacttggtg tcagcttgct ttgggtttgg tcatagtaac caacctcaac attagcgcca
     2641 aagcgctttt ctcccttgat aaacggaatt tgatccacga ttgatttgat aaaggttgat
     2701 tttccgatac cattgggacc aacgatagca acagcattca tcttacgaag gtctaggtta
     2761 atcggttgtg acaggacttc cccatcatag ccaacagccg cattttcaac agtcaaaaca
     2821 acattgcccg acgttttttc agactggaag gtcatgttgg ctgctttctt gccagcttcc
     2881 ggcttgtcca aacgctccat tttttctagt tgtttacggc gagattgagc acgtttggtc
     2941 gttgaagcac gaactagatt gcgattgaca aagtcttcca gagcagcgat ttccttctgt
     3001 tgcttttcat agttttttgc ctcagtagct agcttttgct ccttcaactc gacaaaacga
     3061 gagtaattcc ccacatagcg atccaaggaa tgcttggtca aatctagcgt aattgtcgca
     3121 accttgtcca agaaataacg gtcgtggctg acgataatga gggcaccgct atagtttacc
     3181 aagtaattct ctagccagga gatggtttca atatccaagt ggttagttgg ctcgtccaag
     3241 accaagagat tgggcttttc aaggagcatt ttagctagag ccaaacgagt attttgacca
     3301 cctgaaagtt cagctatttt catctgccac atagactcgt caaacttgaa tccattcaaa
     3361 atcgctcgaa tatcagcttc ataggtaaag ccacctgctt ggcgaaaatt ctcagataag
     3421 cggtcataat ctgacatcag tttatccaaa tcctcaccag acttttcacc catctccagc
     3481 tccatctgac gcagttgtct ctccgtccga cgcaaatcat taaagacatg aagcatttca
     3541 tcgtagatgg tattttcaga ctcaaaacgg ctatcttggg ctaggtaaga cagagaaata
     3601 tcttttttct tattgatttc tccgctagtt ggctcctctt ctccaactaa aatcttcaaa
     3661 agagtagact tacctgcacc atttttccca acaagagcaa tccgatctcg ttcatcaacc
     3721 tgcaggttga tattatcgaa aagaacctct cctgcaaaag aacgttcaat tttattagct
     3781 tgtaaaataa tcataagagt agtatagcat gtttccctaa ggcattcaag ataatcgtaa
     3841 gtcttttagt acaactttta taacataaaa taaactaaat tatgtatatt ttatattaga
     3901 ttccttcact atcttgttgg attttctaac cagctaatct tgtttcaaat agttatcgca
     3961 caagcctatt atttaattct tttcattatt tacgtacgcg tatagcagat tgaaataaga
     4021 tgagaacaaa tcgattggga aagtaaaatt aatttctata aatgttttag caattgtttc
     4081 gtactatttt agattcagtc tactatgtac aatattttcg gaacattcaa ctttttaact
     4141 ctatttatta ctagatttca taattaaaaa gcctactgac caagctagaa agcttgatac
     4201 aataggcttt ttaaagactg attatttaac agcgtcttta agagctttac cagctttgaa
     4261 tgctggtact ttagaagctg caattgtcat ttctttacca gtttgtgggt tgcgaccttt
     4321 acgttctgca cgctcacgaa cttcaaagtt accaaaaccg atcaattgaa ctttttcacc
     4381 agctgcaaga tagtcagcta ctgctgcaaa tacagcttca actgctgctg ctgagtcttt
     4441 cttagtcaat tctgtagctt ctgctacttt agcgatcaaa tcttgtttgt ttgccatgtt
     4501 aatgattcct ccagataatt tctaattaac aaatataatc atatcctaaa atccctttta
     4561 ggtcaagtta aaaacgctat ttcttccaat tttatttatt ttttaggagt ggtaacgtat
     4621 caaaatagcc caagcgttct cacccgtgtg agtttgaata atggaacccg tttccaaaac
     4681 agaaattggc ttttcaacat aagcttgtaa gctttctttc atctcttttg cccaatcatc
     4741 actaccagaa tatgaaattc caatctctgc tacagcacgt tcagaaagcg atgttatcaa
     4801 ctcatctaac cattttttaa atgttttagt tccacgacct ttaaccattg gctgcaattc
     4861 atggtctttc atttgcatga caacacggat attgagaagg gagctcaaca agccagttac
     4921 acggccaatt cgtccacctt tgacaagatt ttccaaagtt gaaacaccaa tataaagctc
     4981 tgtatggttt ttaacctctt ctacatgaga taaaattgcc tccatatctt taccttcttg
     5041 agctaacttc gcagcctcaa caacttggaa tttcagggct tggtcagtga aggaactatc
     5101 aacaacaatc acatctgcag tagataggct agcaccttgg cgtgctgctt ctaccgtacc
     5161 cgaaagagca tgggacatat gaatagcaag aatctggcca ccatctttgc ataggtcttc
     5221 aaaaatctca gcaaagacac ctacaggtgg ctgacttgtt ttcggaagat tcttactttc
     5281 ttgcatcaac tgaagaaatt taccttcttc tttcaaatcc gcatcagaat aaacaacatt
     5341 atcaatcatt acagataatg gaacaattgt aatatctaat tgctttacta gttcaggttc
     5401 aatagtaaca gatgaatcgg ttacaatctt aatttttgtc atagtatcaa tctttctatt
     5461 ttaggattca gattggtttc cttacttcta attatatcaa aaaaaagatt aaaaatccta
     5521 atggagtcaa tcaaattttc cgtaaaattt gatataatca acttataaga aaagaggtgt
     5581 cctatgatta aaaaaattta ccccattttt accattttac taggtgctgc tatttatgct
     5641 tttggactga cttattttgt agttccccat catctctttg aaggaggggc gacaggtatt
     5701 accctcatca cattttatct ttttaaaatc cctgtttccc tcatgaacct gctgattaat
     5761 attccccttt tcatcctagc ttggaagatc tttggagcca aatccctcta ttctagttta
     5821 ctaggaacct tagctttgtc cggctggtta gctttttttg agcatattcc ccttcatatt
     5881 gatcttcaag gtgatttact aatcacagcc cttatagcgg gaatcctatt gggaattggc
     5941 cttggaatta tttttaatgc tggaggtaca actggcggaa ctgatattct agctcgtatt
     6001 ctcaacaaat acactcatat atccatagga aaactgctct ttatcttaga tttttgtatt
     6061 ctcatgttga ttctcctaat cttcaaggat ttgagattgg tttcctacac gcttttgttt
     6121 gattttattg tttctcgtgt tattgatttg attggtgaag gaggatatgc cggcaaaggc
     6181 tttatgatta tcacaaaacg tcctgaccaa cttgctaagg cgattaatga tgacctcgga
     6241 agaggtgtta cttttatttc tggtcaaggc tactatagta aagaaaattt gaaaatcatc
     6301 tactgtattg tcggaagaaa tgaaattgtg aaaacgaagg aaatgattca tcgaatcgat
     6361 cctcaagcct ttataactat tacagaagcc catgaaatcc taggagaagg cttcaccttt
     6421 gaaaaagaat aaaaagaggt aatgtcgtga cctcaaaagt tagactaaat catctatctt
     6481 ttgggttaca gacaacctct tttttgtttt atttactcaa gctcttaaga ccaattccga
     6541 gttacttctt catcagcctt taactgatcc actaattggt caactgagtc aaatttggtc
     6601 atatcacgaa tgcgatcaag ccaataaacc atgacggttt ccccataaat atcttgatta
     6661 aaatcaaaaa tattgacttc aaaacgtgct tcttctccat caaaggtcac atttttcccg
     6721 acactagcca tagcacgata cttctgtctt tgaatctcaa catcaacgac ataaacgcca
     6781 tctgctggca tataagtacg gtctaaaagc actaaattcg ctgtcggata accaattgta
     6841 cgaccacgag cattaccatg aaccaccata cctcttgatg gaagcggtgc ccccaaaagt
     6901 tttcctgctt ctttcacatt tccatctaaa atagcttgac ggatacgagt tgaactaatc
     6961 tttcctttct catcttctac aggtggaaca atgataactt ctccatcaaa gtaatccttt
     7021 aaatcttctg ctgttttttt gtcagaacca aatgtataat caaaacctgc aacaataatt
     7081 ttggcattca tagccttgat ataagttgca aagaattctt gtgcagtgag actagcgaat
     7141 tgactactaa aatcaaggag atataattct tctacacctt cgcgctttaa ttttctttca
     7201 cgttcagcag ggttcaaaat atgcaaaaac aaatccggat gataaggctc taaagcgatc
     7261 tttggagatt cattaaaggt cataacgacg ataggtaata aatcctttct cgcagccttg
     7321 ttggcaacac gaaataattc ttgatgcccc ttatgtatgc catcaaaata gccgagaaca
     7381 acgactgaat cagatggtgt gccaatatct ttttggtttt ttataggaat agtaataatc
     7441 ataaaataat tatatcatag cgatagctat ttctggaaca gaaaatctga aatgttgttt
     7501 ttttcacatg aagtgtacct gttttcaaaa agcactttat tctatcgttg cttaactatg
     7561 aactttgcaa tattcttctc aaaaacttgt aggacatctt caaaattttg caaggagtga
     7621 ttagacttgt tcggtaacca taaagtgtca tactatgtta tgtatgaaaa agcaatgcaa
     7681 ctaactcctg agaactttaa attactaatt ggtgccgaaa aggtagaatt tagaatcgag
     7741 gtacacctat ggctgtaaaa tttacaaaat gagacaactt gggcaagatg tttgaagaat
     7801 ttcctaaact ccctgatttg aagcaagtca ctttccctga tgacaaagaa aaaagccaaa
     7861 acagcaaaga aaaactagat gactgctttc caacaactcc catctagtgt gcttcagact
     7921 gggctatttt tctctccatc tgttagcttg gattctcaga ccgtttcagc taaagaatat
     7981 cttttccctt atcagaagga acggctcaaa ccattcagac aagtgaaggg acgacaagcc
     8041 aatatttgaa accagatagc agttcttata gtcaattgaa ataaaatctg aagaaatcga
     8101 gtaggaaact catatcaatt tttaacagtg ttctattcca gattcatact caatgaaaat
     8161 taaagtgcaa actaggaagt tagccgcagg ctgta
//
LOCUS       DAVBCW010000087         8179 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVBCW010000087 DAVBCW010000000
VERSION     DAVBCW010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8179
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.087"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            110..1735
                     /gene="treC"
                     /locus_tag="VQ514_001633"
     CDS             110..1735
                     /gene="treC"
                     /locus_tag="VQ514_001633"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HET2500372.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTGNGIGDFRGIIGKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCIGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNPNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKDINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            1877..2125
                     /locus_tag="VQ514_001634"
     CDS             1877..2125
                     /locus_tag="VQ514_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HET2500373.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            2332..3126
                     /gene="racE"
                     /locus_tag="VQ514_001635"
     CDS             2332..3126
                     /gene="racE"
                     /locus_tag="VQ514_001635"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HET2500374.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIYVEHVEL"
     gene            3123..4094
                     /locus_tag="VQ514_001636"
     CDS             3123..4094
                     /locus_tag="VQ514_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HET2500375.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGKSSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            4070..4591
                     /locus_tag="VQ514_001637"
     CDS             4070..4591
                     /locus_tag="VQ514_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET2500376.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWLEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            4588..5049
                     /locus_tag="VQ514_001638"
     CDS             4588..5049
                     /locus_tag="VQ514_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET2500377.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            5040..5780
                     /gene="xerD"
                     /locus_tag="VQ514_001639"
     CDS             5040..5780
                     /gene="xerD"
                     /locus_tag="VQ514_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HET2500378.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            5780..6508
                     /locus_tag="VQ514_001640"
     CDS             5780..6508
                     /locus_tag="VQ514_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HET2500379.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            6527..7096
                     /locus_tag="VQ514_001641"
     CDS             6527..7096
                     /locus_tag="VQ514_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HET2500380.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKSQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            7083..7805
                     /locus_tag="VQ514_001642"
     CDS             7083..7805
                     /locus_tag="VQ514_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HET2500381.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            7805..8047
                     /gene="yidD"
                     /locus_tag="VQ514_001643"
     CDS             7805..8047
                     /gene="yidD"
                     /locus_tag="VQ514_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HET2500382.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
BASE COUNT         2364 a         1632 c         1949 g         2234 t
ORIGIN      
        1 ctgtggctag cttcctagtt tgctctttga ttttcattga gtataattga aatagcacaa
       61 atagggacgg aagactgctt cctccctttt atcagaaaag gagaaacaga tgacacttga
      121 taaaggaaaa gtagtctatc aaatctatcc aaaatcttac aaagacacca ctggcaatgg
      181 tattggggat ttccgtggga ttatcggaaa aattccctat ctagccaaac taggtgtgga
      241 tatggtctgg ctcaatccat tctatccaag tcctcaacgg gataatggtt acgatatttc
      301 agattatatg gcagtggatc ctctttttgg tgatatggct gattttgagg aaatggtgtg
      361 tatcggtaaa gagcacaaga ttgactttat gctggacatg gtacttaatc attgttcgac
      421 agagcatgaa tggtttcaga aagccctagc tggtgataag tattatcaag actttttctt
      481 catccaagac caaccaacag attggcagtc taagtttggt ggttctgcat gggcgccttt
      541 cggggatact gggaaatact atcttcacct atttgatgag acccaggctg accttaactg
      601 gcgcaatccc aatgtccgta aggagctttt caaggttgtt aatttctggc gcgacaaggg
      661 tgtcaaaggt tttcgatttg atgtgatcaa tttgattggt aaggacgagg tttcagtgga
      721 ctgtcctgaa aatgaaggga agccagctta tacagacaaa cccatcgttc ataactattt
      781 gcgtatgatg aaccaagcca cttttggatc tgacgatagc tttatgacag ttggggaaat
      841 gtcttctacc accatggaaa actgtgtcct ctattcgtcg cctgatcgtc aggaattatc
      901 catgaccttt aattttcatc acctcaaggt ggactataag gatggacaaa agtggacctt
      961 ggctcccttt gattttgaag agttgaagag tctttaccat agttggggta aggaaatgag
     1021 tgataaagac ggttggagtg ccctcttttg gaacaatcac gatcaaccac gtgccttgaa
     1081 ccgttttgtc gatattcaaa acttccgcaa ggaaggagcc accatgctag cagccagcat
     1141 tcacctgtca cgtgggacac cttatatcta catgggtgag gaaatcggga tgattgaccc
     1201 agactatgat tctatggctg attacgtgga tgtcgaatcg cttaatgcct atcagatgct
     1261 cctagaagaa ggaaagagtc agcaagaagc cttccagatt attcaagcta agtcgcgtga
     1321 taattcacgc attcccatgc agtgggatgc ttcggaaaat gcaggatttt caacaggcac
     1381 tccttggttg aaagctggta aatcctataa agacatcaat gtagaaaatg aaatccaggg
     1441 tccgattttt accttctatc aagacttgat tcgactgcgt aaggaaatgc caatcatttc
     1501 agaaggaagt tacaaaccag cctttgaaga tagcaaacaa gtctacgctt ttgaacgcca
     1561 gtttgaggat caaaagttac tagtgctcaa taacttttat gctaaagaag tggaaatcga
     1621 cttgccagca gtctaccaaa atggacagat tttgatttca aactatgaag atgcagaagt
     1681 gtctgagaaa attctactaa aaccctatca aacactggct atttatgtaa attaaactta
     1741 gcaaggagac ctctttttca gaaaagcagg gttctttttg ctgtctattc catccctgaa
     1801 agtgtagaac aaactggtca gggtggctag tttgtggtat aatgaaaggg actcaaaaag
     1861 aaacaggaga aaaatgatgg atttactttt agcaattgta ttgattgtgc tagcttttct
     1921 aggaggagct cttggaggaa tgtacttggt tcgtaagcaa atcgaaaaag aatttgctga
     1981 caacccacgt ttgaatgctg aagcagttcg tactcttttg agtgcaaatg gtcaaaaacc
     2041 aagcgaagct aaggtacaac aagtttacca ccaaatcatc cgccaacaaa aggcagccct
     2101 tgctaacaat aaaaagaaaa aataaataag gaaaagtctg ggatgaaagt tccagacttc
     2161 tcactatgtt ggttatggtt tagggatgag actttttcct tgcattctat tgatatttga
     2221 ttatgattaa gagagaggta gttgacattc tatcaagctg tcggtcagtt cgttcagtac
     2281 aaggaatcat aatgaacgat caatcagaaa aaagactaga aagaagactg tatggataat
     2341 cgaccaattg gttttttgga ttcgggtgtc gggggcttga ccgttgtgcg cgagctcatg
     2401 cgccagcttc cccatgaaga aatcgtctat attggagatt cggcgcgggc gccctatggc
     2461 ccccgtccag ctgagcaaat tcgtgaatat acttggcagc tggtcaactt tctcttgacc
     2521 aaggatgtca aaatgattgt cattgcttgt aacactgcga ctgcggtcgt ctgggaagaa
     2581 atcaaggctc aactagatat tcctgtcttg ggtgtaattt tgccaggagc ttcggcagcc
     2641 atcaagtcca gtcaaggtgg gaaaatcgga gtgattggaa cgcccatgac ggtacaatca
     2701 gacatatacc gtcagaaaat ccatgatctg gatcccgact tacaggtgga gagcttggcc
     2761 tgtcccaagt ttgctccctt ggttgagtca ggtgccctgt caaccagtgt taccaagaag
     2821 gtggtctatg aaaccctgcg tcccttggtt ggaaaggtgg atagcctgat tttgggctgt
     2881 actcattatc cactccttcg ccctattatc caaaatgtca tggggccaaa ggttcagctc
     2941 atcgatagtg gggcagagtg cgtacgggat atctcagtct tactcaatta ttttgaaatc
     3001 aatcgtggtc gcgatgctgg accactccat caccgttttt acacaacagc cagtagccaa
     3061 agttttgcac aaattggtga agaatggctg gaaaaagaga tttatgtgga gcatgtagaa
     3121 ttatgacaaa taaaatttat gaatataagg atgaccagga ttggtatgtt gggtcttata
     3181 gtatttttgg tggcgttaac agtttgagcg actataagac agattttcct ctgtttgaat
     3241 tctccaaaat atttggagat gaagagtatg gtttcccgct ttcagttact gttttacgct
     3301 atggttctac ctaccgtttg ttctcctttg tggtagacat gcttaatcaa gaaatgggac
     3361 gaaacttgga agttattcaa cgtcatgggg ccctgctctt ggttgaaaat gggcaactct
     3421 tgtatgtaga attgcctaaa gaaggggtca atgttcatga tttctttgag acaagcaagg
     3481 tcagagaaac cttgttgatt gcgactcgta acgaaggcaa aaccaaggaa ttccgagcta
     3541 tctttgataa gttaggctac gatgtggaaa atcttaatga ctaccctgac ctgcctgaag
     3601 tagcagaaac aggtatgacc tttgaagaaa atgcccgcct taaggcagaa accatttctc
     3661 aattaacggg caagatggtt ttggcagatg attctggtct caaagtcgat gtccttggtg
     3721 gcttaccagg cgtctggtca gctcgtttcg caggtgtggg agcaactgac cgtgaaaata
     3781 atgccaaact cttgcacgaa ttggccatgg tctttgaact caaggaccgc tcggctcagt
     3841 tccacacaac cctagtcgta gccagcccaa ataaggaaag tttagttgtt gaagcagact
     3901 ggtcaggtta tattaacttt gaacctaagg gtgaaaatgg ctttggctat gatcccctct
     3961 tccttgtagg agagacaggt aagtcatcag ctgaattaac cctggaagaa aaaaatagtc
     4021 aatctcaccg tgccttagcc gttaagaaac ttttggaggt atttccatca tggcaaagca
     4081 aaccatcatt gtaatgagcg attcccatgg cgatagcttg attgtggaag aagtccgtga
     4141 tcgctatgtg ggcaaagtcg atgccgtttt tcataacggc gattctgaac tacgtccgga
     4201 ttctccactt tgggagggca tccgcgttgt taaaggaaac atggacttct acgccggcta
     4261 cccagaacgt ctggtgactg agcttggttc gaccaagatt atccaaactc atggtcactt
     4321 gtttgacatc aatttcaact ttcaaaagtt ggactactgg gctcaggagg aagaggccgc
     4381 tatctgcctc tatggtcact tgcatgtgcc aagtgcttgg ttggaaggca agatcctctt
     4441 tctaaatcca ggttctatca gtcaaccacg aggtaccatc agagaatgtc tctatgctcg
     4501 tgtggagatt gatgatagtt acttcaaagt ggactttttg acacgagatc acgaggtgta
     4561 tccaggtttg tccaaggagt ttagccgatg attgccaagg agtttgagac tttcttgttg
     4621 gggcaggagg aaactttttt gacccctgct aaaaatctag ctgtgttgat tgatacccac
     4681 aatgcggatc atgcgaccct cttgctcagt cagatgacct atacccgtgt tcccgttgtg
     4741 acagatgaaa aacagtttgt tgggacgatt ggactcagag atattatggc ttatcagatg
     4801 gagcatgact tgagccaaga aatcatggcg gatacggata tcgttcatat gacaaaaacg
     4861 gacgtagcgg ttgtttcgcc tgatttcacc attacggagg tcttgcacaa gctagtagat
     4921 gagtccttct taccggttgt ggatgcagag ggtattttcc aagggattat tacgcgtaag
     4981 tccatcctca aggccgttaa tgccctcttg catgacttta gtaaggaata tgagattcga
     5041 tgccaatgag agacaggatt tcagcctttt tagaggaaaa gcagggcttg tctgtcaatt
     5101 ccaagcagtc ctataagtat gatttggagc aatttttaga catggtaggt gagcggattt
     5161 ctgagaccag tctcaagatt taccaagccc agctagccaa tctaaaaatc agcgcccaga
     5221 agcgaaagat ttcggcctgt aaccaatttc tatactttct ctatcaaaaa ggagaggtgg
     5281 acagctttta ccgcttggaa ttagccaaac aagctgaaaa gaagacggaa aagccagaga
     5341 ttctatacct agactctttt tggcaggaaa gcgaccatcc agagggccgc ttgctagcgc
     5401 tcttaatcct agaaatgggg ctcttgccca gtgagatttt agccatcaag gttgcggaca
     5461 tcaatctgga ttttcaggtg ttgcgaatca gcaaggcttc ccaacagagg attgtcacca
     5521 ttcccacggc cttgctttca gaattggaac ccttgatggg gcagacctat ctttttgaaa
     5581 gaggagagaa accctattct cgtcagtggg cctttcgtca gttagaatct tttgtcaagg
     5641 agaaaggttt tccatcctta tcagctcaag tcttacgtga acagtttatt ctaagacaaa
     5701 tagaaaacaa ggtcgatttg tacgaaattg caaaaaaatt aggattaaaa acagtcctga
     5761 ccttagaaaa atatagataa tggatattaa attaaaagat tttgaaggcc ccctggactt
     5821 gctcttgcat ctggtttcta agtaccagat ggatatctac gatgtgccca ttacggaagt
     5881 catcgaacag tatctagcct atgtttcaac cctgcaggcc atgcgtctgg aagtgacggg
     5941 tgagtacatg gtcatggcta gtcagctcat gctgattaag agtcgtaaac tccttccgaa
     6001 ggtagcagaa gtgacagact tgggggatga cctggagcag gacctcctct ctcaaatcga
     6061 agaatatcgc aagttcaagc tcttgggtga gcacttggaa gccaagcacc aagaacgggc
     6121 ccagtattat tccaaagcgc cgacagagtt gatttacgaa gatgcggagc ttgtgcatga
     6181 caagacgacc attgacctct ttttggcttt ttcaaatatc ctagccaaga aaaaagagga
     6241 gtttgcacaa aatcacacga ctatcttgcg ggatgagtat aagattgagg acatgatgat
     6301 tatcgtgaaa gagtccttga ttggacgaga tcaattgcgc ttgcaggatt tgttcaagga
     6361 agcccagaat gtccaagagg tcatcaccct ctttttggca accctagagt taatcaaaac
     6421 ccaggagctg atcctcgtgc aagaggagag ttttggagat atctatctca tggaaaagaa
     6481 ggaagaaagt caagtgcctc aaagctagac ttgatagaga ggaaagatga gtactttagc
     6541 aaaaatagaa gcgctcttgt ttgtagcggg tgaagatggg attcgggtcc gccagttagc
     6601 tgaactcctc tctctgccac cgacaggcat ccagcagagt ttaggaaaat tagcccagaa
     6661 gtatgaaaag gacccagatt ccagtttggc tttgattgag acaagtggtg cttatagatt
     6721 ggtgaccaag tctcaatttg cagagatttt gaaggaatac tctaaggcgc ctatcaacca
     6781 gagcttgtct cgggctgccc ttgagacctt gtccattatt gcctacaaac agccgattac
     6841 gcggatagaa attgatgcca tccgtggagt taactcgagt ggagccttgg caaagttgca
     6901 ggcttttgac ctgataaagg aagacgggaa aaaggaagta ttggggcgcc ccaacctcta
     6961 tgtgactacg gattatttcc tagattacat ggggataaac catttagaag aattaccagt
     7021 gattgatgag cttgagattc aagcccaaga aagccaatta tttggtgaaa ggatagaaga
     7081 agatgagaat caataagtat attgcccacg caggtgtggc cagtaggaga aaagcagaag
     7141 agctgattaa gcaaggcttg gtgacggtta acggccaagt ggtgcgtgaa ctagcaacca
     7201 ctatcaagtc aggcgacaag gtcgaagttg aaggtcaacc tatctacaac gaagaaaagg
     7261 tctactatct gcttaacaaa ccacgcggtg tgatttccag tgtgacagat gataagggtc
     7321 gcaagacggt tgtcgacctc ttgcccaatg tcaaagagcg tatttaccct gtgggtcgtt
     7381 tggactggga tacatcaggt gtcttgattt tgaccaatga tggggacttt acagacgaga
     7441 tgattcaccc tcgtaatgag attgacaagg tttatgtcgc gcgtgttaag ggtgtggcca
     7501 ataaggacaa tctccgcccc ttgacccgtg gtcttgagat tgatggtaag aaaaccaagc
     7561 cagctgttta tgaaattctc aaagtggacc cagtcaaaaa tcgctctgtg gtgcagttga
     7621 ccatccatga agggcgtaac catcaggtta aaaagatgtt tgaagctgtt ggtctccaag
     7681 tagataagtt gtctcggact cgtttcggac acctagactt gacaggactc cgtccaggag
     7741 aatcccgtcg tcttaataaa aaagaaatca gccaactaca caccatggct gtaactaaga
     7801 aataatgaaa cgaattttaa tagcgcctgt gcgcttttac caacgtttta tctcaccagt
     7861 ctttccaccc tcttgtcgct ttgagctgac ttgttccaac tacatgattc aggctattga
     7921 aaaacatggt tttaaggggg tattgatggg cttggctcgg attttacgtt gtcatccctg
     7981 gtcgaaaaca ggtaaggacc ccgttccaga ccacttttcc cttaaacgaa atcaagaagg
     8041 ggaatgaggt ggggtaaata gatttcaaaa tgataaaaac gcatcctatc aggtttgagt
     8101 gaacttgata ggatgcgttt tagaatgtca aaattttata ctcttcgaaa atcccttcaa
     8161 accgcgtcag ctttcatct
//
LOCUS       DAVBCW010000088         7565 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVBCW010000088 DAVBCW010000000
VERSION     DAVBCW010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7565
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.088"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            119..778
                     /locus_tag="VQ514_001644"
     CDS             119..778
                     /locus_tag="VQ514_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HET2500383.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRIIEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            803..1870
                     /locus_tag="VQ514_001645"
     CDS             803..1870
                     /locus_tag="VQ514_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2500384.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
     gene            1974..2984
                     /locus_tag="VQ514_001646"
     CDS             1974..2984
                     /locus_tag="VQ514_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2500385.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            2996..4687
                     /locus_tag="VQ514_001647"
     CDS             2996..4687
                     /locus_tag="VQ514_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET2500386.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            4690..5400
                     /locus_tag="VQ514_001648"
     CDS             4690..5400
                     /locus_tag="VQ514_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HET2500387.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
     gene            5496..5864
                     /locus_tag="VQ514_001649"
     CDS             5496..5864
                     /locus_tag="VQ514_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500388.1"
                     /translation="MKDYKINFDLGKIEYFDNNCLIQVYKFISFYDICEMVFAFHLPP
                     DELITNVIFKEKINSMLKCYIDRLLYVFINPTHFTEKVNLQFYGSFFSYEFICREVGN
                     ILKNKGVKCNLNFFEGEEYL"
     gene            5972..6973
                     /locus_tag="VQ514_001650"
     CDS             5972..6973
                     /locus_tag="VQ514_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2500389.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
BASE COUNT         2510 a         1022 c         1438 g         2595 t
ORIGIN      
        1 cttttaatga aagataaatt aaaaaaaatg tttgacagcg cttgacaaag gaaacggttt
       61 cattgtataa tgtaaacaca atgacaccga tgtcaaatat aaaaaagaag gtctttatat
      121 gaaaaactta acaaaaatta agttcaaaga aaatggggaa tttaatcatt tccctgggaa
      181 tacagttgta gcaaatcttt atactaaaca agatttgatg gaagttgttg atattattca
      241 atcacgttat agagaattac catttatcga taagtttact ttaactccaa ggaattctat
      301 tcacatgact gtaattgaat tgttgtgcca tgaaaatcgt gaaacggagt tttggagcag
      361 taatcttcct ctagatacac ctttacagga aatacatgat tactttgcta aacaacttga
      421 aatttttcca ttgttggatg aagaaattca tatgcgtata attgaaatgg gaaaacaaaa
      481 catactagtt gaacctgcag atgaagcttc tgcaaagaga ttagaagaaa ttcgtactta
      541 tgtctcagaa aaagcaggtg tttgtttccc taatcatgat agatatcaat tccatatttc
      601 aattgggtat cttcggattc ctctaaccga agaggaggaa gaagagttta ctaaagtcag
      661 agcagaatta actgaaattt tattagagaa gattccaact attactgtaa accgtattga
      721 ctatactgta tttgaagata tgaggcaatt tgttccatat cacgaaaaat ttaaataaaa
      781 taataaaaaa ggagaactta tcatgaaaaa aatgaaagtt tggtctactg tacttgcaac
      841 gggagttgct cttactacac ttgctgcttg ctctggaggt tcaaattcta cgactgcttc
      901 ttcatctgaa gaaaaagctg ataaaagtca agaattagtt atctattcga actcagtctc
      961 aaatggtcgt ggtgattggt taactgctaa agcaaaagaa gctggtttta atataaaaat
     1021 ggttgatatc gctggcgctc aattagcaga ccgtgttatt gctgagaaga ataatgcagt
     1081 tgcagatatg gtatttggaa ttggtgctgt tgactcaaat aaaattagag atcaaaaatt
     1141 actagtacag tacaagccta aatggttaga taaaattgat caatctttat cagataaaga
     1201 taattattat aatcctgtga ttgttcaacc attagtttta attggggcgc ctgatgtaaa
     1261 agaaatgcct aaagattgga ctgaattagg tagtaagtat aaaggtaaat attcaattta
     1321 tggtcttcaa ggtggtacag gacgggcaat tctagcaagt atcttagttc gttaccttga
     1381 tgaaaaagga gatttaggcg tatcagaaaa aggatgggaa gttgcaaaag aatactttgc
     1441 aaatgcctat actcttcaaa aaggtgaaag ctcagttgtt aaagcgttag ataaagaaag
     1501 cccaattcaa tacggtatga tctggggttc tggagcgtta attggacaaa aagaacaaaa
     1561 tgttgaattc aaagttatga ctcctgagat tggtgtacca tttgtaactg aacaaactat
     1621 ggttttaagc actagtaaaa aacaagcgtt agctaaagaa tttattgatt ggtttggtca
     1681 atcagaaatt caagtagaat atagtaaaaa atttggatct attcctgcca ataaagatgc
     1741 cctaaaagag ttacctgaag atacgaaaaa atttgttgat caagtgaaac cacaaaatat
     1801 tgactgggaa gctgttggaa agcatttgga tgaatgggta gaaaaagctg aattagaata
     1861 cgtaaaataa gaaataagag ttatttttta tgtccaagtc tctctattat ttctaaattt
     1921 aaggaagaaa taaagagagg cttttggttt tagtaataga gggagttagt gagatgatta
     1981 aatttgataa tattcaaatt aaatatggtg attttgttgc aattgataat ctgaatttag
     2041 atatacatga aggggaattc tttacatttc ttgggccttc aggatgtggt aaatcaacta
     2101 ctttgagagc attggtaggt tttttagatc catcatcagg aagtattgaa gttaatggaa
     2161 cagatgtcac tcatttggaa cctgaaaagc gtggaattgg tattgtattt caatcttatg
     2221 cgctatttcc aactatgact gtttttgata atattgcatt tggtttaaaa gttaagaagg
     2281 tagctccaga tgttattaaa gctaaagtat cagcagtggc agcaaaaatt aagatctctg
     2341 atcaacagtt acagcgtaat gtatcagaat tatctggggg tcaacaacaa agggtagcat
     2401 tggctcgtgc tctggttctt gaacctaaaa ttctttgtct agatgaacca ttgtcaaacc
     2461 ttgacgcaaa attacgtgta gatttgagaa aagagttgaa aagacttcaa aaagagttag
     2521 gtattactac tttatatgtt actcatgatc aagaggaagc cttgacttta tctgatagaa
     2581 ttgcagtctt taacaatgga tacatcgaac aggtcggtac accagtagag atttatcata
     2641 attctcaaac tgaatttgta tgtgatttta ttggagatat taatgttttg accgatgaaa
     2701 cagtccacga agtattactg aaaaatacaa gcgttttctt agaggataaa aaaggttaca
     2761 ttcgattaga gaaagttcga ttcaatcgtg aaactgaaca agattttatt ctaaaaggga
     2821 caattattga tgttgagttt tctggagtta caattcacta tacaataaaa gtttctgaaa
     2881 gtcagattct taatgtaaca agtattgata gtcaggctgc tattagatct gtcggagaaa
     2941 gtgtggaatt atttatcaca ccatcagacg ttctgcaatt ttaaggaggt gcagtatgcg
     3001 tcataaatta aatttaaaag attggcttat tcgtttaggg ttaatctggt tcttagtaac
     3061 atttattatt tatccaaact ttgatctagt agtgaatgta tttgtaaaag gaggagaatt
     3121 ttcccttgat gctgtacatc gtgttctaaa atctcagagg gcacttcaga gtattatgaa
     3181 cagttttaag ttagcatttt cactcattat tacagttaat gtcgtaggta ttctttgtgt
     3241 tctatttaca gagtactttg atattaaagg tgctaaaatt ttaaaattag gttatatgac
     3301 ctctttaatt tatggaggag tggttttagc gactggatat aaatttgtct atggtcctta
     3361 tggattgatt acaaaatttt tacaaaatgt tatcccttct ttagactcta actggtttat
     3421 tgggtatggt gcagtcttat tcattatgac attttcagga actgctaatc atacattgtt
     3481 tttaacaaat acaattcgaa gcgttgacta tcacactatt gaggctgctc gaaatatggg
     3541 agcaaaacca tttactgttt tccgaaaagt agtgttacca accttaattc caactctatt
     3601 tgcacttact attatggttt ttcttagtgg tttatctgca gtagcagcac ccatgattgt
     3661 tggtggtaaa gaatttcaaa ctataaatcc aatgattatt acatttgcag ggatggggaa
     3721 ttctcgtgat ttagctgcct tacttgcaat tattttaggt attgcaacta caattttgct
     3781 tactatcatg aataagatag aaaaaggtgg aaattatatt tctatctcta agactaaagc
     3841 gcctcttaaa aaacaaaaaa ttgcgtctaa gccttggaat atcattgctc acattgtagc
     3901 atatggattg ttcacagttt tcatgcttcc actaattttt atagtattat actcatttac
     3961 agatccagtt gcaattcaaa caggtaactt aacattatca aactttactt tagaaaatta
     4021 tcgcttattc tttagtaata gtgcggcatt ctctccattc ttggtcagct ttatttattc
     4081 tattattgct gcgacaacag caacaattct cgcagttgta tttgctcgtg ttgtcagaaa
     4141 acataaatct cgttttgatt tcttatttga atatggtgct ctacttcctt ggttactacc
     4201 aagtacactt ttagcagtaa gtttattatt tacttttaat cagccacaat ttcttgtctt
     4261 gaatcagatt ttggtaggta gtttggtaat tctacttatt gcatatatag ttgtaaaaat
     4321 cccattttct tatagaatgg tacgtgctat tttatttagt gttgatgatg agatggaaga
     4381 tgcagcaaga agtatgggtg cttcaccttt ttatactatg atgaaggtta tcattccatt
     4441 tattttaccg gttgttctct ctgttattgc tttaaacttt aactctttat taactgactt
     4501 cgacttatct gtattccttt accaccccct agctcaacca ttaggtatta cgattcgatc
     4561 tgcaggtgat gaaacagcaa catctaatgc acaagctctg gtatttgttt atacaattgt
     4621 tctgatgatt atttctggaa cggtattata cttcacacaa agaccggggc gtaaagtaag
     4681 gaaataatca tgacagccac tagtcttggg ttatcaaata ttgaaatagt tgtcaggatt
     4741 gttttatcag tagtcattgg tagtataatt ggtttagaga gagggagcaa atcccagcct
     4801 gcaggcatcc gaacttatag tattgtttgt ctagctgcat gtttgattat gatgacgaat
     4861 gaatacgtat cttataaatt tgggacagga gatcctacac gattaggagc tcaagttata
     4921 tcaggtgtgg gttttctagg cgctggaacg attcttatta cagataaaaa gaaaattaca
     4981 ggtctgacaa ctgcagcagg catttgggct tcggcaggaa ttggattagc tattggagta
     5041 ggtttttatg agggagctct tttagtagcc atttctgttt ggggtgtgat atccatgttc
     5101 caaccactaa aaaaatatct acaaaatcgt tctaaaatga ttgaattgta tatagtagtt
     5161 aaatctacag aagcctataa tcgtgtattg gtttattgtg cagaaaatgg tatcagaatg
     5221 accgattcaa gaacagcgtt tggagatgtt aattctgaca gaattgaata ctttgatgtt
     5281 ccagataaaa aaatagcgtc atttattact ctggaattat caggtagatt tgaacatctt
     5341 cgactgatgg aagaaattgc taatattgta ggtgttattt atgtcgaaga aattagctga
     5401 aatttagtaa acaaagggac agatgttttt gtccttttgt tttttagata ttttaatcat
     5461 ctaatttttg agtagatttt tgctcagagg tttttatgaa agattataaa atcaattttg
     5521 atttaggtaa aatagaatat tttgataata attgtttgat acaagtatac aaatttattt
     5581 ctttttatga tatttgtgaa atggtgtttg cctttcattt acctcctgat gagttgataa
     5641 caaatgttat ctttaaagaa aaaattaact ctatgttaaa atgttacatt gacaggctat
     5701 tatatgtttt tataaatcct acacatttta ctgaaaaagt taatctacaa ttttatggta
     5761 gttttttctc atatgaattt atttgtcgtg aagttggaaa cattcttaaa aataaaggtg
     5821 tgaaatgcaa tttaaacttt tttgaagggg aagaatattt gtaaaattaa ttttgtgaat
     5881 ttgtggatct taaataattt actaaattct cttgttaaaa aaaattaaat attgtatagt
     5941 aatattagaa tgtttgtaat agaggactga aatgaaaaat ataaatggga aaaaggtaac
     6001 tatttatgat attgcacgtt tatcaggttt ttctccaaaa acagtttcac gagttattaa
     6061 tggtggagta aatgttaaag aagaaactta tcaagcgatt caaaaagtta tagaggaatt
     6121 gtcgtatatt ccaaatgcgt atgcaaaaaa tttaactaaa aaagaagcta taaatatatt
     6181 gatttctgta aaaaaaatcg actcttttcc tttaatttgg tttcatacgc tgttagataa
     6241 agtgttacgg acgtgtaaag aatttggtgt taatgctata gttgagtact ttggagagga
     6301 ggatacaatt agtaattcaa ttatatcaag cacaggtagc ctagtagatg gtgttattgt
     6361 tttttatgaa agtgtagatg atatcagaat tcagtattta aagaagaacc atatgccttt
     6421 tcttgttttc ggtgaatctc aaacgtctgg agtagtctat gtatctaata ataattttca
     6481 agctacttat gatatgatga aagctgtaac tgaagaaaaa tttaaaaata tgtggttgct
     6541 tatgggagga gaatcccatg ttaataagga tcgtgaaaga ggtgtgcgtt cttttctgaa
     6601 tgataaaaat tactttatgg atttaaaagt catttatggc ttatctacaa ttgattcagt
     6661 ttattcttat gcaatgcagc acttgactac ccaaaattat ccggacatta tttttgtttc
     6721 aggagatgaa aaggttcagg gcttgattcg tgcgtgctat gagaaaggaa ttcttattcc
     6781 agatgatatt tctatcattg gatttgataa tattcctatt tctcagtatt atactccagc
     6841 tttgtctaca attgctccca attatgttaa attggctaaa gaaatgatag aaggtgtttt
     6901 agcaattatt aaaggggaaa gtgtcacatc tgttgaagtt tctcctaaat ttgttaggag
     6961 acagagtttc tagtattgtt taattcttga aaatcctagt ggaaaagatg gagtgttcta
     7021 aaataagttc tgtttagcct ccattcagtt tctattgagt tggatccaat ttctaacaag
     7081 tgggacatgt tcatgttcga tctaaggaag atatggtcac attcgaatga tatcaaaaaa
     7141 atcgtaaaaa agtcttgaca aagaaaaaac aaagtattaa aataagttca acaaatcaga
     7201 aaagtaacta tttttgatct tcagggagcc tgtggtgatt gtgaataggt ggttggaagt
     7261 agtgaaagtg ggctgatttt gaaaatgaat ttgaaacaat gaaaattcgg tgcgcacacc
     7321 ttacagtgca gcttgttgtt aaacaaggca gagatgtaaa gggatagtcc ctttataatt
     7381 gaggtggcac cgcgttacca acgccctcac acggaagtat attctgtgtg tgggcttttt
     7441 tctatccgtc gtttggttta tcttttatta gggccttaag tcgctttgat gaacttgagt
     7501 tctatctaca gctccttgcc tcctactaaa agtaaacaaa aaggctggac taatacgaaa
     7561 aaagc
//
LOCUS       DAVBCW010000089         7549 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVBCW010000089 DAVBCW010000000
VERSION     DAVBCW010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7549
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.089"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            172..1563
                     /locus_tag="VQ514_001651"
     CDS             172..1563
                     /locus_tag="VQ514_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET2500390.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKIEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIDQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKHQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            1677..2519
                     /locus_tag="VQ514_001652"
     CDS             1677..2519
                     /locus_tag="VQ514_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET2500391.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQEQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     IAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERETLKTIAVQSGAPRVD
                     DVDF"
     gene            2792..3553
                     /locus_tag="VQ514_001653"
     CDS             2792..3553
                     /locus_tag="VQ514_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET2500392.1"
                     /translation="MDNLYLVKDDSQLATFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSDFNAATQIIRESAVPAYTNNRRMVMAPDLAVWKELYLYQLMDYECSQQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEHFLDDFDGYDSYIWFEEGMVEYISRKYFLTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWRSFLTVDKLVENLG
                     SVQAVLDSYHLWANTEKTFPLLDWFVQQKLIEKEI"
     gene            3923..4840
                     /gene="glyQ"
                     /locus_tag="VQ514_001654"
     CDS             3923..4840
                     /gene="glyQ"
                     /locus_tag="VQ514_001654"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HET2500393.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRVKLLAEDAE"
     gene            5100..7136
                     /locus_tag="VQ514_001655"
     CDS             5100..7136
                     /locus_tag="VQ514_001655"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HET2500394.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQAVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSELFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            7178..7435
                     /locus_tag="VQ514_001656"
     CDS             7178..7435
                     /locus_tag="VQ514_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HET2500395.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
BASE COUNT         2365 a         1325 c         1688 g         2171 t
ORIGIN      
        1 aggtctacta ttctagcttc aatctactag aaattccata gatagaaaac tacataatct
       61 ctacagatac ggatgttgga gttgatgtaa gatgctttgg cttgctagag gaattgtgga
      121 ttgccaaatt gtatcattga aattattgct caaatttgtt atgatataaa tatgaataaa
      181 agtagactag gacgtggcag acacgggaaa acgagacatg tattattggc tttgattggt
      241 attttagcaa tttctatttg cctattaggc ggatttattg cttttaagat ctaccagcaa
      301 aaaagttttg agcaaaagat tgaatcgctc aaaaaagaga aagatgatca attgagtgag
      361 ggaaatcaga aggagcattt tcgtcagggg caagccgaag tgattgccta ttatcctctc
      421 caaggggaga aagtgatttc ctctgttagg gagctgataa atcaagatgt taaggacaag
      481 ctagaaagta aggacaatct tgttttctac tatacagagc aagaagagtc aggtttaaag
      541 ggagtcgtta atcgtaatgt gaccaaacaa atctatgatt tagttgcttt taagattgaa
      601 gagactgaaa agaccagtct aggaaaggtt cacttaacag aagatgggca accttttaca
      661 cttgaccaac tgttttcaga tgctagtaag gctaaggaac agctgataaa agagttgacc
      721 tccttcatag aggataaaaa aatagatcaa gaccagagtg agcagattgt aaaaaacttc
      781 tctgaccaag acttgtctgc atggaatttt gattacaagg atagtcagat tatcctttat
      841 ccaagtcctg tggttgaaaa tttagaagag atagccttgc cagtatctgc tttctttgat
      901 gttatccaat cttcgtactt actcgaaaaa gatgcggcct tgtaccaatc ttactttgat
      961 aagaaacatc aaaaagttgt cgctctaacc tttgatgatg gtccaaatcc agcaacgacc
     1021 ccgcaggtat tagagaccct agctaaatat gatattaaag cgactttctt tgtgcttggg
     1081 aaaaatgttt ctgggaatga ggacttggtg aagaggataa aatctgaagg tcatgttgtt
     1141 ggaaaccata gttggagcca tccgattctc tcgcaactct ctcttgatga agctaaaaag
     1201 cagattactg atactgagga tgtgctaact aaagtgctgg gttctagttc taaactcatg
     1261 cgtccacctt atggtgctat tacagatgat attcgcaata gcttggattt gagctttatc
     1321 atgtgggatg tggatagtct ggactggaag agtaaaaatg aagcatctat tttgacagaa
     1381 attcagcatc aagtagctaa cggctctatc gttttgatgc atgatattca cagtccgaca
     1441 gtcaatgcct tgccaagggt cattgagtat ttgaaaaatc aaggttatac ctttgtgacc
     1501 ataccagaga tgctcaatac tcgcctaaaa gctcatgagc tgtactatag tcgtgatgaa
     1561 taagcaagaa aaaataggtc tgttagatat ttgacagact tattttttac agaatatagt
     1621 actacttaaa aaatgtttta tgctataatt gatgaataaa atagaaggag aagcatatga
     1681 atacctatca attaaataat ggagtagaaa ttccagtatt gggatttgga acttttaagg
     1741 ctaaggatgg agaagaagcc tatcgtgcag tgttagaagc cttgaaggct ggttatcgtc
     1801 atattgatac ggcggcgatt tatcagaatg aagaaagtgt tggtcaagca atcaaagata
     1861 gcggagttcc acgtgaagaa atgttcgtaa ctaccaagct ttggaatagt cagcaaacct
     1921 atgagcaaac tcgtcaagct ttggaaaaat ctatagaaaa actgggcttg gattatttgg
     1981 atttgtattt gattcattgg ccgaacccaa aaccgctcag agaaaatgac gcatggaaaa
     2041 ctcgcaatgc ggaagtttgg agagcgatgg aagacctcta tcaagaaggg aaaatccgtg
     2101 ctatcggcgt tagcaatttt cttccccatc atttggatgc cttgcttgaa actgcaacta
     2161 tcgttcctgc ggtcaatcaa gttcgcttgg cgccaggtgt gtatcaagaa caagtcgtag
     2221 cttactgtcg tgaaaaggga attttattgg aagcttgggg gccttttggt cagggagaac
     2281 tatttgatag caagcaagtg caagaaatcg cagcaaatca cggaaaatcg attgctcaga
     2341 tagccttggc ctggagcttg gcagaaggat ttttaccact tccaaaatct gtcacaacct
     2401 ctcgtattca agctaatctt gattgctttg gaattgaact gagtcatgag gagagagaaa
     2461 ccttaaaaac gattgctgtt caatcgggtg ctccacgagt tgatgatgtg gatttctaga
     2521 aaatcataaa aagaattgta cattattcta atttttgata taatagtcag caggaaagaa
     2581 agtcttatgg cgttcttcaa gcgagcttgg gatagtggga gccaagtagg gcaaaataat
     2641 gagctggcgc tttctgtagt attttcaaaa acaatgaagt aataaattag ggtggaaccg
     2701 cgtttctgac gcccctaggt taaatcaacc taggattgtc ggacgcggtt cttttgctta
     2761 ttcagtctat tgtgtgaaag aaaggagagc cgtggacaac ctttatcttg taaaagacga
     2821 tagtcaacta gctacatttc gtgattttgt agtaagaaat actgaaaagt tgaaagatta
     2881 tcaatctttt ttaaagaatg aacttgcagt ctgtgattta ccgcaagccg ttatttggtc
     2941 agattttaat gctgctacac agattattag ggaaagcgct gttccagcct atacaaataa
     3001 tagacgaatg gttatggcgc ctgatttagc tgtttggaaa gagttgtatt tgtatcaatt
     3061 gatggactac gagtgttctc agcaaactca agcaatagaa agtcactatc attctttatc
     3121 tgaaaatttc ctcttacaga ttgtaggaca tgagttagct cattggtcgg agcatttttt
     3181 agatgatttt gatggttatg actcttatat ctggttcgaa gaggggatgg ttgaatatat
     3241 tagtcgcaag tatttcttga cagaagagga atttcaagcg gaaaaaattt gtaatcaatc
     3301 tctcgtagaa ctttttcaga agaagtatgg ttggcattca ttgaatgatt ttggttcttc
     3361 gacttatgat aagaactatg caagtatttt ttatgaatac tggcgcagct ttttgacagt
     3421 agataagttg gtagaaaatt taggtagtgt acaagcggtc ttagattctt atcatttatg
     3481 ggcaaataca gaaaaaactt ttcccttgtt agattggttt gttcagcaga aattaattga
     3541 aaaagaaata taaaaactaa aggagtaaac aatgtctaag aaattgacat ttcactgcgt
     3601 cagtggcaga aacctcctta cagtcggact gccccacgct cagcactaga gtgcctgagc
     3661 tagacgcagt actaactcgt cttgcctcgt atgatcgacg aggcagactc atgtcgcaag
     3721 taattatttt ttattaagga gtaaacaatg tctaagaaat taacatttca ctgcatcagt
     3781 ggcagaaacc tccttacagt cggactgccc cacgctcagc accctagtgc tgagctagac
     3841 gcagtactaa ctcgtcttgc ctcgtataat cgacgaggca gactcgtgtc gcaagaaatc
     3901 attttttatt aaggagtaaa caatgtctaa gaaattaaca tttcaagaaa ttattttgac
     3961 tttgcaacaa ttttggaatg accaaggttg tatgcttatg caggcttatg acaatgaaaa
     4021 aggtgcgggg acaatgagtc cttacacttt ccttcgtgct atcggacctg agccatggaa
     4081 tgcagcttat gtagagccat cacgtcgtcc tgctgacggt cgttatgggg aaaaccctaa
     4141 ccgtctctac caacaccacc aattccaggt ggtcatgaag ccttctccat caaatatcca
     4201 agaactttac cttgagtctt tggaaaaatt gggaatcaat cctttggagc acgatattcg
     4261 ttttgttgag gacaactggg aaaacccatc aactggttca gctggtcttg gttgggaagt
     4321 ttggcttgac ggaatggaaa tcactcagtt cacttatttc caacaagtcg gtggattggc
     4381 aactggccct gtgactgcgg aagttaccta tggtttggag cgcttggctt cttacattca
     4441 agaagtagac tctgtctatg atatcgagtg ggctgatggt gtaaaatacg gagaaatctt
     4501 tatccagcct gagtatgagc actcaaaata ttcatttgaa atttcggacc aagaaatgtt
     4561 gcttgaaaac tttgataagt ttgaaaaaga agctggtcgt gcattagaag aaggcttggt
     4621 acaccctgcc tatgactatg ttctcaaatg ttcacatacc tttaatctgc ttgacgcgcg
     4681 tggtgccgta tctgtaacag agcgtgcagg ctatatcgct cgtatccgta acttggcccg
     4741 tgttgtagcc aaaacctttg ttgcagaacg caaacgccta ggttacccac ttttggatga
     4801 agaaacacga gttaaacttt tagcagaaga cgcagaatag tgagtaatac tgtactctaa
     4861 aatcgttaga ggcaagtcga agacttgcta gagggatatg caccgccgta ctgttatgtg
     4921 gggatttttg aaaccttagg ttcaaaaatc agtcagctaa atccactttg atgagattgt
     4981 tggaaatctt gataaattta gcattggatt tccatacaga ctcacagagt tagttagcga
     5041 cgtccccagt acctaaggtg catatcaaaa aagattgaag aaaatgtaaa ggaaaaaaca
     5101 tgacaaaaaa cttattagta gaactcggtc ttgaagaatt accagcctat gttgtcacac
     5161 caagtgaaaa acaactaggc gaaaaaatgg cagccttcct caaggaaaac cgcctgtctt
     5221 ttgaagccat tcaaaccttc tcaacaccac gtcgtttggc tgttcgtgtg actggtttat
     5281 cagacaaaca gtctgatttg acagaagatt tcaagggacc agcaaagaaa attgccttgg
     5341 atagtgatgg aaactttacc aaagcagctc aaggatttgt ccgtggaaaa ggcttgactg
     5401 ttgaagatat cgaattccgt gaaatcaagg gtgaagaata tgtctatgtc actaaggaag
     5461 aagttggtca agcagttgaa gccattgttc ctggtgttgt agatgtcttg aagtcattga
     5521 ctttccctgt cagcatgcac tgggctggaa atagctttga atacattcgt cctgtccaca
     5581 ctttaactgt tctcttggat gagcaagagt ttgacttgga tttccttgat atcaagggaa
     5641 gtcgtgtgag ccgtggccat cgtttcttgg ggaaagaaac caaaattcag tcagcattga
     5701 gctatgaaga agaccttcgt aagcagtttg taatcgcaga tccatgtgaa cgtgagcaaa
     5761 tgattgttga ccaaatcaag gaaattgagg caaaacatgg tgtacgtatc gaaattgatg
     5821 cggatttgct gaatgaagtc ttgaatttgg ttgaataccc aactgccttc atgggaagtt
     5881 ttgatgctaa ataccttgaa gttccagaag aagtcttggt gacttctatg aaggaacacc
     5941 agcgttactt tgttgttcgt gatcaagatg gaaaactctt gccaaacttc atttctgttc
     6001 gtaacggaaa cgcagagcgt ttgaaaaatg tcattaaagg aaatgaaaaa gtcttggtag
     6061 cccgcttgga agacggagaa ttcttctggc gtgaagacca aaaattggtg atttcagatc
     6121 ttgttgaaaa attaaacaat gtcaccttcc atgagaagat tggttccctt cgtgaacaca
     6181 tgattcgtac gggtcaaatc actgtacttt tggcagaaaa agctggtttg tcagtggatg
     6241 aaacagttga ccttgctcgt gcagcagcca tttacaagtt tgacttattg acaggtatgg
     6301 ttggtgaatt tgacgaactc caaggaatta tgggtgaaaa atacaccctt cttgctggtg
     6361 aaactccagc ggtggcagct gctattcgtg aacactacat gcctacatca gctgaaggag
     6421 aacttccaga gagcaaggtc ggcgcagttc tagccattgc agacaaattg gatacgattt
     6481 tgagtttctt ctcagtagga ttgattccat caggttctaa tgacccttat gcccttcgtc
     6541 gtgcaactca aggtgtggtt cgtatcttgg atgcctttgg ttggcacatt gctatggatg
     6601 agctgattga tagcctttat gcattgaaat ttgacagttt gacttatgaa aataaagcag
     6661 aggttatgga ctttatcaag gctcgtgttg ataagatgat gggctctact ccaaaagata
     6721 tcaaggaagc agttcttgca ggttcaaact ttgttgtggc agatatgttg gaagcagcaa
     6781 gtgctctcgt agaagtaagc aaggaagaag attttaaacc atctgttgaa tcactttctc
     6841 gtgcctttaa cctggctgag aaggcagaag gggttgctac agttgattca gaactatttg
     6901 agaatgacca agaaaaagct ttggcagaag cagtagaaac actcgtttta tcaggacctg
     6961 caagtcagca attgaaacaa ctctttgcgc ttagcccagt cattgatgct ttctttgaaa
     7021 atactatggt aatggctgaa gatcaggctg tccgtcaaaa tcgtttggca atcttgtcac
     7081 aactaaccaa gaaagcagct aagtttgctt gttttaacca aattaacact aaataaaatt
     7141 tgataaacgg actttatctt attacaaagg agaagaaatg gatccgaaaa aaattgctcg
     7201 tatcaatgag cttgctaaaa agaaaaaaac agaaggctta acaccagaag aaaaagtgga
     7261 acaagccaaa ctacgtgagg agtacatcga aggttatcgc cgcgctgttc gtcaccacat
     7321 tgaaggaatc aaaattgtgg acgaagaagg aaacgatgtt acaccagaaa aactacgcca
     7381 agtacaacgt gaaaaaggat tacatggccg tagtcttgat gacccaaatt cataatatgt
     7441 actctttctt ttaacaaaga tagatgaaac gataacaaag agactagcag tttgtgtttg
     7501 ctagtctttt ttcgctaaaa aaggaaccat aatggttcct aaaaactat
//
LOCUS       DAVBCW010000090         7367 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVBCW010000090 DAVBCW010000000
VERSION     DAVBCW010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7367
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.090"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            58..927
                     /locus_tag="VQ514_001657"
     CDS             58..927
                     /locus_tag="VQ514_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET2500396.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            931..1887
                     /locus_tag="VQ514_001658"
     CDS             931..1887
                     /locus_tag="VQ514_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET2500397.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKLPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            1887..2651
                     /locus_tag="VQ514_001659"
     CDS             1887..2651
                     /locus_tag="VQ514_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2500398.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            2651..3361
                     /locus_tag="VQ514_001660"
     CDS             2651..3361
                     /locus_tag="VQ514_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2500399.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKIPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            3720..4376
                     /locus_tag="VQ514_001661"
     CDS             3720..4376
                     /locus_tag="VQ514_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET2500400.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKIGILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDESIDLPALREKFEANGIQVEEIARTSAKVL
                     "
     gene            4484..5579
                     /gene="prfB"
                     /locus_tag="VQ514_001662"
     CDS             join(4484..4555,4557..5579)
                     /gene="prfB"
                     /locus_tag="VQ514_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HET2500401.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQDKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            5597..6289
                     /gene="ftsE"
                     /locus_tag="VQ514_001663"
     CDS             5597..6289
                     /gene="ftsE"
                     /locus_tag="VQ514_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HET2500402.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            6282..7208
                     /locus_tag="VQ514_001664"
     CDS             6282..7208
                     /locus_tag="VQ514_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2500403.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKI"
BASE COUNT         2174 a         1274 c         1654 g         2265 t
ORIGIN      
        1 aacctatcaa aaagtgaggg aaaaccctcg gaattataaa tagaaagagt gaatcttatg
       61 ctccaacaac tcgtaaatgg tttgattcta ggtagtgttt acgcgctgtt agccctagga
      121 tataccatgg tttacggaat tatcaagctc atcaacttcg cccatggtga tatttatatg
      181 atgggagcct ttatcggtta tttcttgatc aattctttcc aaatgaattt ctttgtagcg
      241 cttattgtag ctatgctagc gacagctatt cttggtgtcg tgattgagtt tcttgcttac
      301 cgacctttgc gccactctac tcgtattgct gttttgatta cggctattgg ggtttctttc
      361 ctattggagt atggaatggt ctatctggtt ggtgccaata cccgtgcctt ccctcaagcg
      421 attcaaacag ttcgatatga tttgggacca attagcttaa caaatgtgca gttaatgatt
      481 ttggccattt ccttgatttt gatgattttg ttacaagtca ttgtccaaaa gactaagatg
      541 gggaaagcca tgcgtgcagt atcagtagat agcgacgcgg cgcaattgat ggggatcaat
      601 gtaaaccgta cgattagctt taccttcgct ttgggttctg ctcttgcggg tgcggctggt
      661 gttctgattg ctctttatta taactctctt gagcctttga tgggggttac tccaggtctt
      721 aaatctttcg ttgccgcagt acttggtggt atcggaatta ttcctggtgc ggctcttggt
      781 ggctttgtga ttggtctatt ggaaaccttt gcgactgcct ttgggatgtc agatttccgt
      841 gatgccattg tttatggaat cttgttgttg atcttgattg tccgcccagc tggtatcctt
      901 ggtaagaatg tgaaagagaa ggtgtaaacg atgaaggaaa atttaaaagt taatattcta
      961 tggttactcc ttttgttagc tggctatagc ttgattagtg tactggtttc agtcggagta
     1021 cttaatctat tctatgtaca gattttacaa caaattggaa ttaatattat tttggctgtt
     1081 ggtctcaact taatcgttgg tttttcagga caattttcac ttggtcatgc tggtttcatg
     1141 gcgattggtg cctatgcagc agctattatt ggttctaaat taccaaccta cggtgccttc
     1201 tttggagcta tgcttgtagg ggctttgctt tcaggagcag ttgccttact tgtcggcatt
     1261 ccaaccttgc gcttgaaggg ggactatctt gcggtagcaa ctctaggtgt ttctgaaatt
     1321 atccgtatct ttatcatcaa tggtggaagt cttacaaatg gtgcggcagg tatcttaggg
     1381 attcctaact ttacaacttg gcaaatggtt tacttctttg tcgtgattac aaccattgca
     1441 accttgaact tcttgcgtag cccaattggc cgttcaaccc tctctgttcg tgaagatgaa
     1501 atcgctgctg agtcagttgg ggttaatacg actaaaatta aaatcatcgc ttttgtcttt
     1561 ggtgccatta ctgcaagtat tgctgggtca cttcaggcag gatttatcgg gtctgttgta
     1621 ccgaaagatt acaccttcat taactcaatc aacgttttga ttattgttgt atttggtgga
     1681 ctcggttcca ttacaggtgc gattgtttcg gctattgttc tgggaatttt gaatatgctt
     1741 ctccaagatg ttgctagtgt gcgtatgatt atttacgctt tagccttggt attggtaatg
     1801 attttcagac caggtggact ccttggaacg tgggaactga gcctatcacg tttctttaaa
     1861 aaatctaaga aggaggaaca aaactaatgg cattacttga agtaaaacag ttaaccaaac
     1921 attttggtgg tctaacagct gttggagatg tgactcttga attgaacgaa ggggaactgg
     1981 ttggattaat cggtccaaac ggagctggga aaaccaccct tttcaacctt ttgaccggtg
     2041 tttatgaacc aagcgaggga acagtaaccc tagatggtca ccttttgaat gggaaatcac
     2101 cttataagat tgcctctttg ggacttggac gtactttcca aaatatccgt ctctttaaag
     2161 atttaacagt tttagataat gttttgattg cttttggaaa ccatcacaaa cagcatgttt
     2221 ttactagttt cttacgctta ccagcttttt acaagagtga aaaagaatta aaggctaaag
     2281 ctttggaatt gttgaaaatc tttgatttag atggtgatgc agagactctt gctaaaaatc
     2341 tttcctacgg acaacaacgt cgtttggaaa ttgttcgtgc ccttgctacg gaacctaaaa
     2401 ttctcttctt agatgaacca gcagcaggta tgaacccaca ggaaacagcc gaattgactg
     2461 agttaattcg tcgtatcaaa gatgaattta agattacaat catgttgatt gaacacgata
     2521 tgaatctggt catggaagta acagaacgta tctacgtact tgaatatggc cgtttaattg
     2581 ctcaaggaac tccagacgaa attaagacca ataaacgcgt tatcgaagct tatctaggag
     2641 gtgaagccta atgtctatgt taaaagttga aaatctttct gtgcattacg gtatgatcca
     2701 agcagtccgt gatgtaagct ttgaagttaa tgaaggagaa gttgtttccc ttatcggtgc
     2761 caacggtgca ggtaagacaa ctattcttcg caccttgtca ggtttggttc gaccaagttc
     2821 aggaaagatt gaatttttag gtcaagaaat ccaaaaaata ccagctcaga aaatcgtggc
     2881 aggtggtctt tcacaagttc cagaaggacg ccacgtcttt cctggcttga ctgttatgga
     2941 aaatcttgaa atgggagctt tcttaaagaa aaatcgtgaa gaaaatcaag ctaacttgaa
     3001 gaaggttttc tcacgctttc ctcgtcttga agaacgtaag aaccaagatg cagctactct
     3061 ttcaggaggg gaacaacaaa tgcttgccat gggacgcgct cttatgtcaa caccaaaact
     3121 tcttctttta gatgaaccat caatgggact tgccccaatc ttcatccaag agatttttga
     3181 tatcattcaa gatattcaga agcaaggaac aaccgtcctc ttgattgaac aaaatgccaa
     3241 taaagcactt gcaatctctg accgaggata tgtattggaa acaggaaaaa tcgtcctatc
     3301 aggaacagga aaagaactcg cttcatcaga agaagtcaga aaagcatatc taggtggcta
     3361 aaacaatcca gtggattgtt ttagtcggca gatgaagatt acgaagtaat catcaatata
     3421 gtccgggggc cctttttagt cggtagattg agattgcgaa gcaatcatca aatccagagg
     3481 attgttttag ccggtagatt gagattgcaa acaaatctgc atctacattg aaagcttaat
     3541 ttctaataat tgaaaaaatc gaatgaaaag tttcttacct tcattcacag agctcgattt
     3601 tagagctctt tttgctagct tattcatact tttctaaatt ttgaaaaaga aatgtaagcg
     3661 tttgatagat ttgcaaaaag attgtataat agggataaga atagaaaagg agaagtctca
     3721 tggcagttaa agattttatg acccgcaagg tagtttatat tagtccagat acaacagtat
     3781 ctcatgcagc agatttgatg agagagcaag gtttgcaccg tctgcctgtt atcgaaaatg
     3841 atcaattggt tggtttggtg actgagggaa ccattgcaca agcaagtcca tctaaagcaa
     3901 caagtctttc tatctatgag atgaattatc ttctgaataa gacaaaagta aaagatgtca
     3961 tgattcgcga tgttgtcact gtctcaggct atgctagtct agaagatgca acttatctga
     4021 tgttgaaaaa taagattggt attctccctg tcgtagataa ccatcaagta tacggagtta
     4081 ttactgaccg tgacgttttc caagcctttc ttgaaattgc aggttatggc gaagaaggga
     4141 ttcgtgtacg ctttgttaca gaagatgaag ttggtgttct tggaaaaatt gtttctttga
     4201 ttgtagaaga aaatttgaat atctcccata cagtcaatat tccgcgtaag gatggtaagg
     4261 tgattatcga agtgcaaatc gatgaatcaa ttgatttacc agccttgaga gaaaaatttg
     4321 aagcaaatgg tattcaagtg gaagaaatcg ctcgcacttc agcaaaagtc ttgtaagaag
     4381 ggaagtccaa aggcttcttt tttcatgaaa aggggattag agcaaaagat ggaaagaaat
     4441 gataaaatat gctataatga aataatgtaa aaaaggagta tttatggaca tttcagtaat
     4501 tcgtcagaaa attgacgcaa atcgtgaaaa attagcttct ttcagggggt ctctttgacc
     4561 tcgaagggct agaggaagag attgccatct tggaaaacaa gatgacagaa cctgattttt
     4621 ggaacgataa tattgcggcc caaaaaacgt cgcaagaatt gaatgaatta aaaaacactt
     4681 acaatacctt ccataagatg gaagagttgc aggatgaagt cgaaatttta ttggattttt
     4741 tggctgaaga cgagtcagtg catgatgaac tggtagcgca gttagccgaa cttgataaga
     4801 taatgaccag ctacgagatg actctactct tgtcagaacc ttatgaccac aacaatgcca
     4861 tcttggaaat ccatccaggt tctggtggta ctgaggcgca ggactggggt gatatgttgc
     4921 ttcgtatgta tactcgttat ggtaatgcta aaggctttaa agtggaagtg ttggattacc
     4981 aagcaggtga tgaggctggt attaagtcgg taactttatc atttgaaggg cctaatgcct
     5041 atggtctcct caagtcagaa atgggtgttc accgcttagt gcgaatctca ccatttgact
     5101 ctgccaaacg tcgccatacc tctttcacat ctgtagaagt gatgccagaa ttggatgata
     5161 ctattgaagt ggaaatccgt gaagatgata tcaagatgga taccttccgt tcaggtggtg
     5221 ccggtggaca aaacgtcaat aaggtttcaa caggtgtacg tttaacccac attccaactg
     5281 gaattgttgt ccaatcaaca gtggatcgta cccagtatgg aaatagagat cgtgccatga
     5341 agatgttgca ggctaagctc tatcaaatgg agcaagataa gaaggctgcg gaggtagatt
     5401 ctctcaaagg tgagaaaaag gagatcactt ggggaagcca aatccgttct tatgtcttca
     5461 cgccttatac tatggtaaaa gatcaccgaa ctagctttga ggttgctcag gtagataagg
     5521 ttatggatgg ggacctagat ggttttatag atgcttatct caagtggcga attagctaag
     5581 atagaaagga actcacatgt caattattga aatgagagat gtcgttaaaa aatacgacaa
     5641 cggaacgact gctctacgcg gtgtttcggt cagcgttcaa ccgggggaat ttgcttacat
     5701 cgtaggacct tcaggagcag ggaagtcaac ttttattcgt tctctgtatc gtgaagtaaa
     5761 aatcgataaa ggaagtctat cagttgctgg ttttaatctg gttaagatca aaaagaaaga
     5821 tgtcccgctt ctacgtcgta gtgttggggt tgtcttccaa gattataaat tgttaccaaa
     5881 gaaaactgtc tatgaaaata ttgcttacgc tatggaagta atcggggaaa atcgccgtaa
     5941 tatcaaaaga cgagtgatgg aagttttgga cttggttgga ttgaagcata aggttcgttc
     6001 tttcccaaat gaactctcag gtggggagca gcagcgaatt gcgattgcgc gtgcaattgt
     6061 aaataatccc aaagtattga tagctgatga gccaacagga aatctggatc cggataattc
     6121 atgggaaatt atgaatctct tggaacggat taacctacaa gggacaacta ttttgatggc
     6181 gactcataat agccagattg taaatacctt gcgccaccgt gtcattgcca ttgaaaatgg
     6241 ccgtgtcgtt cgtgacgaat caaaaggaga gtatggatac gatgattagt agattttttc
     6301 gccatttatt tgaagcctta aaaagtttga aacgaaatgg ttggatgaca gtagctgctg
     6361 tcagttcagt catgattact ttgaccttgg tggcaatatt tgcatctgtt attttcaata
     6421 cagcgaaact agctacagat attgaaaata atgtccgtgt agtagtttat atccgaaagg
     6481 atgtggaaga taatagtcag acaattgaaa aagaaggtca aactgttaca aataatgact
     6541 accacaaggt atatgattct ttgaagaaca tgtctacggt taaaagtgtt accttttcaa
     6601 gtaaagaaga acaatatgaa aaattaaccg agataatggg agataactgg aaaatctttg
     6661 aaggagatgc caatcctctc tatgatgcct atattgtaga ggcaaacact ccaaatgatg
     6721 taaaaactat agccgaagat gctaaaaaaa ttgaaggtgt ctctgaggtt caagatggcg
     6781 gtgccaatac agaaagactc ttcaagttag cttcatttat ccgtgtttgg ggactaggga
     6841 ttgctgcttt gttaattttt atcgcagttt tcttgatttc aaataccatt cgtattacca
     6901 ttatttcccg cagtcgcgaa attcaaatca tgcgcttggt tggagctaaa aacagttata
     6961 tccgtggacc gttcttgtta gaaggagcct ttatcggttt attgggagct atcgcaccat
     7021 ctgttttggt ctttattgtt tatcaaattg tttaccaatc tgtcaacaaa tcgttggtag
     7081 ggcaaaatct atccatgatt agtccagatt tatttagtcc gttgatgatt gccctactat
     7141 ttgtgattgg ggttttcatt ggttcattgg gatcaggaat atccatgcgc cgattcttga
     7201 agatttaggt aaaatagctg cttttatgag gagattgtaa aatctccttt tttgctacaa
     7261 gagtttttga aaagagatgc gtagaagaaa agagcttcca aagaagtccc ccagagaaga
     7321 cttcttgatg gctaatttga acttgaaatt tggcgcacta gaaattt
//
LOCUS       DAVBCW010000091         7361 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVBCW010000091 DAVBCW010000000
VERSION     DAVBCW010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7361
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.091"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(<1..825)
                     /locus_tag="VQ514_001665"
     CDS             complement(<1..825)
                     /locus_tag="VQ514_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2500404.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKSTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIIAGISNGA"
     gene            complement(839..1705)
                     /locus_tag="VQ514_001666"
     CDS             complement(839..1705)
                     /locus_tag="VQ514_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2500405.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSIVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVIS
                     IIQLRVSKKFEI"
     gene            complement(1719..2978)
                     /locus_tag="VQ514_001667"
     CDS             complement(1719..2978)
                     /locus_tag="VQ514_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2500406.1"
                     /translation="MEWYKKIGLLATTGLALFGLGACSNYGKSADDTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRSE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            complement(3070..5232)
                     /locus_tag="VQ514_001668"
     CDS             complement(3070..5232)
                     /locus_tag="VQ514_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HET2500407.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATITSYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIATLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIILSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHEIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMIRYFPQVWASDNTDAIARLPIQYGSSYFYPTISMGA
                     HVSAVPNHQMGRMTPLETRGHVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            5340..6200
                     /locus_tag="VQ514_001669"
     CDS             5340..6200
                     /locus_tag="VQ514_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET2500408.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFYYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKSPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            6197..7132
                     /locus_tag="VQ514_001670"
     CDS             6197..7132
                     /locus_tag="VQ514_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HET2500409.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTAIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
BASE COUNT         2348 a         1528 c         1329 g         2156 t
ORIGIN      
        1 tgccccgttg cttattcctg cgatgatatg gcgttggaag aagagataga caatggtgat
       61 actgataatg ccgaccacgt aagaggcaaa gcttggtccg tagtcgttga aatattggcc
      121 tgcgtagttg tattggaaca aaggcagagt ccacattttg gaatcccggt tcaagacaag
      181 gagtggcaac atgaagtcat tccagaacca aagggcattg atgatcatgg ttgtcgcatg
      241 catcggtttc atcattggga agatgatgcg gaaataggtt gtaaattgat tagccccatc
      301 gatctctgct gcttcatcca gactttctgg aatcgagatt ttgatatagc caacatagag
      361 aaagagggtc tgtggaatcg cataggtcaa gtagagcaag atcaaaccaa aggtattagc
      421 caaaccgagt ttactcatca taaccgtaat cggaatcatg atgacttgga aaggtacgaa
      481 gattccgagg attaagaggg tatacatgat ggtaaaggct tttcttttac tcatattgcg
      541 agcgatggag taggctgcca tagggataaa gatcattact gcaagtaaag acaagacagt
      601 gatgacgaca gagttccaat aatagcctcc aatcccatca gctaagagac ggctaaagtt
      661 gtcccatgtg aagttggttg gaaagccaaa gaaattatct acaatatcct tagtggattt
      721 gaaggaacta aagagggtag caaggagcgg cactaaaatc agaaccgatc ctagaatcaa
      781 tagaatgtat ttgccaatca gggcttttct ttcatcttgt ttcatcatgc ttctcctctt
      841 aaatttcaaa tttcttagat actctcaatt ggatgatcga aatcactaca attaagaaga
      901 acaagattac ggcaatggca ttggcataac cgaattggtt gtttttaaag gcatagttat
      961 aaaccaagag cccaagtgag gttgtggcat tgtttggacc accaccggtc atggcaaaga
     1021 cttggtcaaa ggcagtcagc ccacctttta gggctaggat aaagactata gagacacttg
     1081 gtagcaagta aggcaattca atgttccaga aaacttgctt gctagtcgca ccatcaatcc
     1141 ttgctgcctc tgtaatctca gttggaatag attgcaaacc agctaggaag atgatgatgg
     1201 gcatagccac cccttgccaa agaaggacaa agacagccgc aaagattgct ccccacttag
     1261 tccctaaaag actggtttgg agaaattcaa tatgaagggc attcccaatc gctggaagac
     1321 cgtagttgaa gacttgcttg aagatcaaag ccactgtcaa accagataaa acagctggga
     1381 agaagaacca agcacggaag aaggtttggc ctttgatttt agagttcaag acacgcgcaa
     1441 tgaagatccc gagtgcaatc tcaccaacca ccatggcaat cgtaatgatt gcggtaaagc
     1501 caatcgcatt catgaatttt ggatccatga agaggagctt aaagttgttt aagccgacaa
     1561 atttgtagtt gtaagtcaat cctgtccagt tggtaaaact gtaaaaggct ccttgaaaca
     1621 tcggcacata gaagaaaatt gcttgtaaca agagggggat gaccacaaaa gcccatgccc
     1681 aatatttttg taatactttt ttcatagtct ctctactcct aatccacatc cgctttcatc
     1741 gggttaaaga aggcattcaa atcattgacc atgccttgtt tatcaccggt caagacatag
     1801 ttcatggtca aggtatggaa gtctgcttca ctggtccagt attgttgcaa ccagaccaag
     1861 tgacgatccg taaaggcata ttcggtcata ccagcaagcg gtgaatcttc tcctgcttgt
     1921 ttgacccctt cgatcgctgt tggagatccg tccacatcgt agtatttttg catgacttct
     1981 gaacgggtca tatattccac aaaggcattg gcttcttttg gatgtttggt ggtggctgag
     2041 atagaccatg ccaagtctcc cgcaccaacg gttaagcttt gtcctttttc ttttcctgga
     2101 atcatgaagg ttccaatctt aaagttcggt ttttgttcat taatcgctgt gatcgcccaa
     2161 gacccatttg gtgtcatgag gacatcccca cgtgcgaagg ctccgataac atcggtatag
     2221 ccagcacctt cccagttctt ttgcttagag ccattaatgc gaaggatgtc catgaccttg
     2281 atatcatctt tcataatcgg atccgacaat ttaatagcat ttggttgaga ataacgaagg
     2341 tattgatttg cttcttttcc tccacctgtt gctgtcgcaa aggctaattg attgtaacca
     2401 ttgagtgtcc aagcatctgc acctgcaatt ccaaatggtg tttgtccttt agcaacgata
     2461 tctttgacta actgttcaaa ttcatcccag gtttcaggaa ccttcaagcc cagttcttcg
     2521 aatttatctt tgttgtagta aattccataa gcattagctg taaaaggaac gttgtaaact
     2581 ttttcgttta cagcatattt ttcagcgtag ccatttttca cgcgtttcag gtagtctttg
     2641 ttactcaaat cttcaaagac acctgctttt gcccattctt gcagttcgat ggactgtggg
     2701 taaatattga ccacatcagg cacatctcct gcgagaacgc gtgtcttcaa tacttcacca
     2761 gcatttggta cattgacgac tttgaccttt atcttagggt tttccttctc aaaatcacga
     2821 gtgatttctt ccaaggtttt ggtcatttct tttttctggt tgaaatactc gatggtcact
     2881 gtgtcatccg cagatttacc atagttggag caagcaccga gcccaaacaa agctaaacct
     2941 gtagttgcaa gaagtccgat ttttttatac cattccatta gaaagcctcc tttataaatt
     3001 tatacaccct tatcaaactg cacttcaaaa gttagataga ataaatctaa cttttggggt
     3061 cagtacatat catagttttc taaaaatata ctgtctactc aaaaaatctc cttgggataa
     3121 gataacagtt aagcccgcat acattagttc tgcacctgag taaacttcgc cattttcctg
     3181 taatttatat agtccctctt catccaaatc ttttaatttt aaagttgttt ccatggtctc
     3241 tacaacagat aaaacgcgaa cgtaggttac aatcgtttga tttccgtaat taaattgtac
     3301 agctgcttca ttggatgcag tatcaggatt aattagtcta tactgctgtc ctaactgaac
     3361 tactggtcgt aattctttat acaagttcac ctgattagca atcgtagctt tctcttcatc
     3421 tgataaattt gtcaaatcaa gctcatagcc caaatttccc atcattgcta catggccacg
     3481 tgtttctaat ggtgtcattc gtcccatctg atgattcggt actgctgaca catgagcccc
     3541 catagaaatg gttggataga aataggatga accgtattga attggtaaac gtgcaatggc
     3601 atcagtatta tcactagccc agacttgtgg gaaatagcgt atcataccaa gatcatttcg
     3661 tccaccacca ccagagcagg actcaaagag aatatggctg tgcttctctg tcagataaga
     3721 aacgagttca taaagcccca gcatgtactg atgagattgc atctgtgtct ctagataagt
     3781 taatccattc cctagcttag tgatattgcg gttcatatcc catttaatgt aatcaatctc
     3841 atgataagat aggagttgat ctaagacact tttcaagtat tctactacct gaggattggc
     3901 aagattaagt actaattgat tccgagaata agtatgctca tagtcaggaa cctgaatagc
     3961 ccagtcaggg tgttgacgat acaaatcact atctacagaa atcatttcgg gttctaacca
     4021 aagtccaaac tgcaaacctc tttcatggat agctgaaatc agactttcta gacttccacc
     4081 cagtttttcc tcattaacaa cccaatcacc taaagcacga ttatcatcaa aacgattgcc
     4141 aaaccaacca tcatctaata caaaaagttc aatgccaact ttcttagctt catctgctaa
     4201 ctctaacagt ttttctctct gaaagtcaaa gtaagtagct tcccagttat tgattagaat
     4261 tggacgttct tttttagaaa attcacttag aataatgtgc ttcagtacaa aattctgact
     4321 ttcatgacta ataccagtta atccctgatc tgaatgagtc actaaagcta ccggtgtttc
     4381 aaagtattcc tcaggggcta acttccaaga aaagttttct ggattaatgc caatagccac
     4441 ccgaacttca ttcaattgat ttttttgaac aaaagcttca aagttgccac tatacattag
     4501 ttgaatagca aacacattcc cagcatcctc tgtgactcct tgttcgcata gtagaagagc
     4561 tggtgtttga gcatgaccag aagcacctcg gtttgaacta atcgaaaaga ttccttgttc
     4621 tacctgttga cgtctaacag tcttttcacg agcataagca ccctgcagag ttgctatttc
     4681 gtaatctgca gctggaaaat cagccataaa agaaaaatct ttatggatga caacttcctg
     4741 attactatta ttatctaatt tactgtagct agtaatagtc gcatcattat taaaagtagt
     4801 ataatacaaa gtcagactaa gttgagcctt agaatcttct aacattaaga caagagtctc
     4861 tgtatcgtcc atgctatgtg gagaaggtaa gccctgtgga ccattctgac cttttaaaat
     4921 ctttgcttct acaaatcgaa agtctgttac ttcagttaca ctatgctgaa cctgtatagt
     4981 tggtttccta aaatctccta agccatgttg tccaaaaatc tgtcgctgag tatctaaact
     5041 aaaggttcga ttagtagccg ttggatttcc tgaaaaggca tggtctcgtt cataaacact
     5101 attggaacct ttatagttct taatagtctt tcctaaatgt ttcaaaagta agtagccatt
     5161 tcgattttca ataatcaaac ttagattttt actctcaaca taaaatagat tattctctat
     5221 cctaactccc atttacttca cctcatcact ttattgatta tattttatca cctgaaatcg
     5281 ctttccaaaa tagaaaaatg tctcaagaat atggtaaaat gttaggtagg aggtagcaca
     5341 tgttagtttt ttcagaatac cagactggaa caatcgacct tgccctaagc ttttatggat
     5401 atgaggaatg cacacctaat tactcttttg gtccagccat tcgtgataca tacgttctac
     5461 attacattac taaaggacaa ggaaaatttt attacaaggg taaaattgtt gatttaaaag
     5521 aaggagattt ctttctatta aaaccagagg aactaacctt ttatcaagca gatagtaaag
     5581 aaccttgggc ctactactgg ttaggaatca ctggagggaa atcccctgat tattttgctc
     5641 tttctcaaat ttctgatcaa tcctatctca tccaatctga aacttgtcat acccagacta
     5701 ctgcaaaact catctcagac attgtccgct tcgctcagat tacaaaatca agtgaattag
     5761 ctcaactcca tatcatggga caacttcatg aactgatgtt tcatctggga actattgctc
     5821 ccaatcagaa aaaaaagaat atttcatcaa cccaccaact ctatcttgaa tgcaaacgat
     5881 taattgatag ccactatcct caatcactta caattcaaga tttagcaaaa gaactatccg
     5941 ttcacagaag ctacttatca agcgtattca aagaatttaa taccttatca cccaaagaat
     6001 acctactcta cgttcgaatg caccgagcta gacaacttct cgaaaatacc caagagtcca
     6061 tcaaggtaat tgcatactcg gtaggttttt cagatccact ccatttttcg aaagcttata
     6121 aacaatactt taatcagact ccaagtcata caagaaaaga atactctcaa taccaactag
     6181 tgagaaaggc aacattatga aatcctacca agctgtctac caaatcctat ctaaagaaac
     6241 cgactatatc agcggagaaa aaatcgcaga aaaactatcc ctaagccgaa cagcaatttg
     6301 gaaagccatc aagcgactag aacaagaagg cattgaaatt gatagtatca aaaatagagg
     6361 atataaactg atgaatggtg accttattct tccagagatt ctagaagaaa atcttccaat
     6421 taaagtcagc tttaaacccg aaacaaaatc aacacaacta gatgcaaaag aagcaattga
     6481 tttaggccat gaagcaaata ccctctatct agcttcctat caaacagcag gccgaggccg
     6541 ttttcaacgt tccttctact caccacaagg tggtatttat atgacactcc atcttaaacc
     6601 aaatctcccc tatgacaaat taccatccta cacactactt gtagctggag ctgtctacaa
     6661 agccattaag aacctaactt taatagatgt cgacataaaa tgggtcaatg atatctatct
     6721 aaacaatcat aaaattggag gaatccttac tgaagcaatg acctctgtag aaactggctt
     6781 agtcacagat atcattattg gagtaggtat caatttcact attaaagact tccctcagga
     6841 attaaaagaa aaagctgcca gcttatttaa agctacagct cctataacaa ggaatgaatt
     6901 gatcatagaa atctggcgtg ctttcttcga aacaccagca gaagagctat tatacctata
     6961 caaaaaacag tcattcattc taggaaaaga agtcactttc acactagagc aaaaagacta
     7021 caagggactt gctaaagaca tctcagaaaa tggaaaactt ttagttcaat gtgataacgg
     7081 aaaagaaatc tggctaaata gtggcgaaat ttctctcaat agttggaagt aaaataacac
     7141 aattataata taaacgatat aaaaataact tcagattagt aattcaatta agttttacgg
     7201 atctgaagtt ttattgtctc taaaaacaaa aaagagagtt acaaactctc attaaaacgg
     7261 gctctataat atttgtagtg ggtaaattcc ctatggatat tatggagcct attttgtgta
     7321 gaaaaaaagt cccatatgac ctataatgaa aagcgacaaa a
//
LOCUS       DAVBCW010000092         7297 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVBCW010000092 DAVBCW010000000
VERSION     DAVBCW010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7297
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.092"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            226..6657
                     /locus_tag="VQ514_001671"
     CDS             226..6657
                     /locus_tag="VQ514_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET2500410.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALNISDKEKV
                     VLDKETENKEKHEDIHNAIETSQDFKEKKTAVIEGKEVVSKNPVTDNNTSNEEARIKE
                     DSNQSQGDHTDSFVNKNTENPKKEDKVVYIAEFKDKESGEKAIKQLSNLKNTKVLYTY
                     DRIFNGSAIETTPDNLDKIKQIEGISSVERAQKVQPMMNHARKEIGVEEAIDYLKSIN
                     APFGKNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKI
                     PHAFNYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQ
                     IFSYKMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRAL
                     RKAGIPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQT
                     VEFDKVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDL
                     RGKIAVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGT
                     KSQVFSISGDDGVKLWNMINPNKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVG
                     DEKEIDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVIN
                     GKSTYGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQ
                     NTARPMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGS
                     ISLKEIKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPYGK
                     QIVPEIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESV
                     EEMEALNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGK
                     PKIPGTLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNQGINAPSSSG
                     SKIANIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVIS
                     REHFIRGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATK
                     IRGQFEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLIT
                     KDTYHKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYA
                     GEGQGRNRKLDKDGNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFANHADEKG
                     MISYYLVDPDKDASKYEKLGEISEDKLKNAKSPEENTNNNQAKDEDSKPDEKSSVEGE
                     ASLEINKTISTIREFENKDLKKLIKKKFREVDDFTSETGKRIEEYDYKYDDKGNIIAY
                     DDGSALQYETEKLDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNSYKSIEI
                     KATKYDSHSKTMIFDLYANINDIVDGLAFAGDMRLFVKDDNQIKAETKIRMPEKNKET
                     KAEYPYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKGY
                     ALKVTTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRSTQ
                     AVLMSALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDKS
                     NVEAIKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNWE
                     LRVNESVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMDG
                     NVYLQTGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKRN
                     NGFDGEIQEQHIYVNGKEYTSFDDIKQITDKTLNIKIVVKDFARNTTVKEFILNKDTG
                     EVSELKPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKKD
                     AGYVINLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDLQ
                     REDHSQKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIVG
                     IFLGLKKKNQD"
     gene            complement(6948..7297)
                     /locus_tag="VQ514_001672"
                     /pseudo
     CDS             complement(6948..7297)
                     /locus_tag="VQ514_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2851 a          985 c         1416 g         2045 t
ORIGIN      
        1 taatcttatt ttttaacgtg tgctaaaaaa taaattaatg catgttaatt tttcttgaat
       61 taaatttttt taaatgatat actgttttta tggagagata acaattatat ataaatgtac
      121 tactctattt cctagataat acttagtaaa actttttata acaaaggcta gcaaagttaa
      181 agtttattat ctattggaag ttaaataaat atgtaaggaa ttaaaatgaa aaaaagtaca
      241 gtattgtcat taaccacagc tgcagttatt ttagcagcct atgcccctaa tgaggtagtc
      301 ttagcagaca catctagctc tgaagatgct ttaaacatct ctgataaaga aaaagtagta
      361 ttagataagg aaacagaaaa taaagagaaa catgaagata ttcataatgc tatagaaact
      421 tcacaggatt ttaaagagaa gaaaacagca gtcattgagg gaaaagaagt tgttagtaaa
      481 aatcctgtga cagacaataa cactagcaat gaagaagcaa gaatcaaaga agattccaat
      541 caatcccaag gagatcatac ggactcattt gtgaataaaa acacagaaaa tcccaaaaaa
      601 gaagataaag ttgtctatat tgctgaattt aaagataaag aatctggaga aaaagcaatc
      661 aagcaactat ccaatcttaa gaatacaaaa gttttatata cttatgatag aatttttaac
      721 ggtagtgcca tagaaacaac tccagataac ttggacaaaa ttaaacaaat agaaggtatt
      781 tcatcggttg aaagggcaca aaaagtccaa cccatgatga atcatgccag aaaggaaatt
      841 ggagttgagg aagctattga ttacctaaag tctatcaatg ctccgtttgg gaaaaatttt
      901 gatggtagag gtatggtcat ttcaaatatc gatactggaa cagattatag acataaggct
      961 atgagaatcg atgatgatgc caaagcctca atgagattta aaaaagaaga cttaaaagga
     1021 actgataaaa attattggtt gagtgataaa atccctcatg cgttcaatta ttataatggt
     1081 ggcaaaatca ctgtagaaaa atatgatgat ggaagggatt attttgaccc acatgggatg
     1141 catattgcag ggattcttgc tggaaatgat actgaacaag atatcaaaaa ctttaacggc
     1201 atagatggaa ttgcacctaa tgcacaaatt ttctcttaca aaatgtattc tgacgcagga
     1261 tctgggtttg cgggtgatga aacaatgttt catgctattg aagattctat caaacacaac
     1321 gttgatgttg tttcggtatc atctggtttt acaggaacag gtcttgtagg tgagaaatat
     1381 tggcaagcta ttcgggcatt aagaaaagca ggcattccaa tggttgtcgc tacgggtaac
     1441 tatgcgactt ctgcttcaag ttcttcatgg gatttagtag caaataatca tctgaaaatg
     1501 accgacactg gaaatgtaac acgaactgca gcacatgaag atgcgatagc ggtcgcttct
     1561 gctaaaaatc aaacagttga gtttgataaa gttaacatag gtggagaaag ttttaaatac
     1621 agaaatatag gggccttttt cgataagaat aaaatcacaa caaatgaaga tggaacaaaa
     1681 gctcctagta aattaaaatt tgtatatata ggcaaggggc aagaccaaga tttgataggt
     1741 ttggatctta ggggcaaaat tgcagtaatg gatagaattt atacaaagga tttaaaaaat
     1801 gcttttaaaa aagctatgga taagggtgca cgcgccatta tggttgtaaa tactgtaaat
     1861 tactacaata gagataattg gacagagctt ccagctatgg gatatgaagc ggatgaaggt
     1921 actaaaagtc aagtgttttc aatttcagga gatgatggtg taaagctatg gaacatgatt
     1981 aatcctaata aaaaaactga agtcaaaaga aataataaag aagattttaa agataaattg
     2041 gagcaatact atccaattga tatggaaagt tttaattcca acaaaccgaa tgtaggtgac
     2101 gaaaaagaga ttgactttaa gtttgcacct gacacagaca aagaactcta taaagaagat
     2161 atcatcgttc cagcaggatc tacatcttgg gggccaagaa tagatttact tttaaaaccc
     2221 gatgtttcag cacctggtaa aaatattaaa tccacgctta atgttattaa tggcaaatca
     2281 acttatggct atatgtcagg aactagtatg gcgactccaa tcgtggcagc ttctactgtt
     2341 ttgattagac cgaaattaaa ggaaatgctt gaaagacctg tattgaaaaa tcttaaggga
     2401 gatgacaaaa tagatcttac aagtcttaca aaaattgccc tacaaaatac tgcgcgacct
     2461 atgatggatg caacttcttg gaaagaaaaa agtcaatact ttgcatcacc tagacaacag
     2521 ggagcaggcc taattaatgt ggccaatgct ttgagaaatg aagttgtagc aactttcaaa
     2581 aacacggatt ctaaaggttt ggtaaactca tatggttcca tttctcttaa agaaataaaa
     2641 ggtgataaaa aatactttac aatcaagctt cacaatacat caaacagacc tttgactttt
     2701 aaagtttcag catcagcgat aactacagat tctctaactg acagattaaa acttgatgaa
     2761 acatataaag atgaaaaatc tccatatggt aagcaaattg ttccagaaat tcacccagaa
     2821 aaagtcaaag gagcaaatat cacatttgag catgatactt tcactatagg cgcaaattct
     2881 agctttgatt tgaatgcggt tataaatgtt ggagaggcca aaaacaaaaa taaatttgta
     2941 gaatcattta ttcattttga gtcagtggaa gaaatggaag ctctaaactc caacgggaag
     3001 aaaataaact tccaaccttc tttgtcgatg cctctaatgg gatttgctgg gaattggaac
     3061 cacgaaccaa tccttgataa atgggcttgg gaagaagggt caagatcaaa aacactggga
     3121 ggttatgatg atgatggtaa accgaaaatt ccaggaacct taaataaggg aattggtgga
     3181 gaacatggta tagataaatt taatccagca ggagttatac aaaatagaaa agataaaaat
     3241 acaacatccc tggatcaaaa tccagaatta tttgctttca ataaccaagg gatcaacgct
     3301 ccatcatcaa gtggttctaa gattgctaac atttatcctt tagattcaaa tggaaatcct
     3361 caagatgctc aacttgaaag aggattaaca ccttctccac ttgtattaag aagtgcagaa
     3421 gaaggattga tttcaatagt aaatacaaat aaagagggag aaaatcaaag agacttaaaa
     3481 gtcatttcga gagaacactt tattagagga attttaaatt ctaaaagcaa tgatgcaaag
     3541 ggaatcaaat catctaaact aaaagtttgg ggtgacttga agtgggatgg actcatctat
     3601 aatcctagag gtagagaaga aaatgcacca gaaagtaagg ataatcaaga tcctgctact
     3661 aagataagag gtcaatttga accgattgcg gaaggtcaat atttctataa atttaaatat
     3721 agattaacta aagattaccc atggcaggtt tcctatattc ctgtaaaaat tgataacacc
     3781 gcccctaaga ttgtttcggt tgatttttca aatcctgaaa aaattaagtt gattacaaag
     3841 gatacttatc ataaggtaaa agatcagtat aagaatgaaa cgctatttgc gagagatcaa
     3901 aaagaacatc ctgaaaaatt tgacgagatt gcgaacgaag tttggtatgc tggcgccgct
     3961 cttgttaatg aagatggaga ggttgaaaaa aatcttgaag taacttacgc aggtgagggt
     4021 caaggaagaa atagaaaact tgataaagac ggaaatacca tttatgaaat taaaggtgcg
     4081 ggagatttaa ggggaaaaat cattgaagtc attgcattag atggttctag caatttcaca
     4141 aagattcata gaattaaatt tgctaatcat gctgatgaaa aggggatgat ttcttattac
     4201 ctagtagatc ctgacaaaga tgcttctaaa tatgaaaaac ttggtgaaat ttctgaagat
     4261 aaactcaaaa atgcaaagag cccagaggaa aataccaata ataatcaagc taaggatgaa
     4321 gattcaaaac cagatgaaaa aagttcagtt gagggagaag ctagccttga aataaataaa
     4381 actatttcaa caattagaga gtttgaaaat aaagacctaa agaaactcat taaaaagaaa
     4441 tttagagaag ttgatgattt tacaagtgaa actggtaaga gaatagagga atacgattat
     4501 aaatacgatg ataagggaaa tattattgct tatgacgatg gtagtgcctt acaatatgaa
     4561 actgaaaaac ttgacgaaat aaaatcaaaa atttatggtg ttctaagccc atctaaagat
     4621 ggacactttg aaattcttgg aaagataagc aatgtttcta aaaatgccaa ggtatattat
     4681 ggaaatagct ataaatcgat agaaatcaaa gcgactaagt atgattcaca ttcaaaaacg
     4741 atgatatttg atttatacgc taatattaat gatattgtag atggattagc ttttgctgga
     4801 gatatgagat tatttgttaa agatgacaat caaataaaag ctgaaactaa gattagaatg
     4861 cctgaaaaaa ataaggaaac taaagcagaa tatccctatg tatcaagtta tgggaatgta
     4921 atcgaattag gagaaggaga tctttcaaag aacaaaccag acaatttaac taaaatggaa
     4981 tctggtaaaa tctattctga ttcagaaaaa caacaatatc tgttaaagga taacatcatt
     5041 ctaagaaaag gctatgcact aaaagtgact acctataatc ctggaaaaac ggatatgtta
     5101 gaaggaaatg gagtctatag caaggaagat atagcaaaaa tacaaaaggc caatcctaat
     5161 ctaagagtcc tttcagaaac aacaatttat gctgatagta gaaatgttga agatggaaga
     5221 agtacccaag ctgtattaat gtcggctttg gacggcttta acattataag gtatcaagtg
     5281 tttacattta aaatgaacga taaaggggaa gctatcgata aagacggaaa tcttgtgaca
     5341 gattcttcta aacttgtatt atttggtaag gatgataaag aatacactgg agaggataag
     5401 tccaatgtag aagctataaa agaagatggc tccatgttat ttattgatac caaaccagta
     5461 aacctttcaa tggataagaa ctactttaat ccatctaaat ctaataaaat ttatgtacga
     5521 aatccagaat tttatttaag aggtaagatt tctgataagg gtggttttaa ctgggaattg
     5581 agagttaatg aatcggttgt agataattat ttaatctacg gagatttaca cattgataac
     5641 actagagatt ttaatattaa gctgaatgtt aaagacggtg acatcatgga ctggggaatg
     5701 aaagactata aagcaaacgg atttccagat aaggtaacag atatggatgg aaatgtttat
     5761 cttcaaactg gctatagcga tttgaatgct aaagcagttg gagtccacta tcagttttta
     5821 tatgataatg ttaaacccga agtaaacatt gatcctaagg gaaatactag tatcgaatat
     5881 gctgatggaa aatctgtagt ctttaacatc aatgataaaa gaaataatgg attcgatggt
     5941 gagattcaag aacaacatat ttatgtaaat ggaaaagaat atacatcatt tgatgatatt
     6001 aaacaaataa cagacaagac actaaacatt aagattgttg taaaagattt tgcaagaaat
     6061 acaaccgtaa aagaattcat tttaaataaa gatacgggag aggtaagtga attaaaacct
     6121 catagggtaa ctgtgaccat tcaaaatgga aaagaaatga gttcaacgat agtgtcggaa
     6181 gaagatttta ttttacctgt ttataagggt gaattagaaa aaggatacca atttgatggt
     6241 tgggaaattt ctggtttcga aggtaaaaaa gacgctggct atgttattaa tctatcaaaa
     6301 gataccttta taaaacctgt attcaagaaa atagaggaga aaaaggagga agaaaataaa
     6361 cctacttttg atgtatcgaa aaagaaagat aacccacaag taaaccatag tcaattaaat
     6421 gaaagtcaca gaaaagagga tttacaaaga gaagatcatt cacaaaaatc tgattcaact
     6481 aaggatgtta cagctacagt tcttgataaa aacaatatca gtagtaaatc aactactaac
     6541 aatcctaata agttgccaaa aactggaaca gcaagcggag cccagacact attagctgcc
     6601 ggaataatgt ttatagtagg aatttttctt ggattgaaga aaaaaaatca agattaagac
     6661 aaaagctata gaaaaaaatg gtttatgtac tgagattaga tagtgaggtg atgacatagt
     6721 tttgtgaaaa cagccattta taactcaatt atttagttta cttcactatt gatactattt
     6781 ggagttatta atggacttag tttagataac taatgaattg attgaaaggg ttagtattga
     6841 caatattggt catattgatt agaaaataga gtctatcaaa atttaaaggc taatagaggt
     6901 gatgagacaa tttcggctct ttgtcaactg tagtgggttg aagtcagcta agctcgagaa
     6961 aggacaaatt ttgtcctttc ttttttgata ttcagagcga tgaaaatccg ttttttgaag
     7021 ttttcaaagt ttcgaaaacc aaaggcattg cgcttgataa gtttgatgag attattggtc
     7081 gcttccagtt tggcattaga atagtgtagt tgaagggcat tgacaatctt ctctttatct
     7141 ttgaggaagg ttttagagga tgaacctgat tcagattgtc ctcaatgagt ccgaaaaatt
     7201 tgtcaggctc cttattctga aagtgaaaaa gcaagagttg atagagatta tagtggtgtt
     7261 tcaagtcttc tgaatagctc aaaagtttat ctatagt
//
LOCUS       DAVBCW010000093         7283 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVBCW010000093 DAVBCW010000000
VERSION     DAVBCW010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7283
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.093"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(<1..207)
                     /locus_tag="VQ514_001673"
     CDS             complement(<1..207)
                     /locus_tag="VQ514_001673"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500411.1"
                     /translation="MENMTNPLLLVQIRVHGYLLDFASSEGQRQKHYRLKNLPQTVEL
                     TVDDVEEDVDLTLPENRSYQEADFF"
     gene            complement(275..916)
                     /locus_tag="VQ514_001674"
     CDS             complement(275..916)
                     /locus_tag="VQ514_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2500412.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            complement(918..2948)
                     /locus_tag="VQ514_001675"
     CDS             complement(918..2948)
                     /locus_tag="VQ514_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HET2500413.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEVLSHLAVEHNSLIARRIVEPNEAGETLFTYATYGEGELPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLAATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQFICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSTYYQRLFTDYL
                     QNFSSESVEVTSQKYYLPQVSLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            complement(3217..4116)
                     /locus_tag="VQ514_001676"
     CDS             complement(3217..4116)
                     /locus_tag="VQ514_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2500414.1"
                     /translation="MLIGQKIKEIRIEKGVSRPYFCGDEQELTVRQLSRIESGASQPS
                     LPKLAYIARRLGVPVYSLMPDFSALPPAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMSQIIEKEVYSTNDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTKLENFFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALREERDFSLAESFYQSSKTFAK
                     LIGDGFLVKKLTEEWQEDVKKYL"
     gene            complement(4188..4748)
                     /gene="rnmV"
                     /locus_tag="VQ514_001677"
     CDS             complement(4188..4748)
                     /gene="rnmV"
                     /locus_tag="VQ514_001677"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HET2500415.1"
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINAQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVRHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(4748..5521)
                     /locus_tag="VQ514_001678"
     CDS             complement(4748..5521)
                     /locus_tag="VQ514_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET2500416.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVTQMNIVGFDKPTIE
                     RALELVDEYDQLYATIGWHPTEAGTYTEEVEAYLLDKLKHPKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKILVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(5826..6719)
                     /locus_tag="VQ514_001679"
     CDS             complement(5826..6719)
                     /locus_tag="VQ514_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HET2500417.1"
                     /translation="MVAQDPKAQDSTKPTAEKSTVKAPAQRVDVKDITHLTDEEKVKV
                     AILQANGSALDGATINVAGDGTATITFPDGSVVTILGKDTVQQSAKGESVTQEATPEY
                     KLENTPGGDKGGNTGSSDANANAGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKN
                     AIEKAAKDKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGV
                     QAIVMVTVPKRPVAPNAAPKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALAS
                     LGLVVATSGFALLGRKTRRRK"
     gene            complement(7003..7137)
                     /gene="rpmH"
                     /locus_tag="VQ514_001680"
     CDS             complement(7003..7137)
                     /gene="rpmH"
                     /locus_tag="VQ514_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HET2500418.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
BASE COUNT         2054 a         1715 c         1254 g         2260 t
ORIGIN      
        1 aaaaaaatca gcttcttgat aacttcgatt ttcgggtagg gtcaaatcca catcctcctc
       61 cacatcatcc actgtcagtt caaccgtttg aggtaaattt ttcaaacgat aatgcttttg
      121 cctttgccct tcactactag caaaatcaag caaatagcca tgcactcgga tttgaaccaa
      181 aaggagagga ttggtcatat tttccatgat gaggatatct tataaaccac ctctggcact
      241 aggataatca gataccctgc gaatacggat tttttcacac atgagcaagt ttcctcatat
      301 caatgatttc atcagcctta ttccagacta ggggattatg ggtcgcaatg atgataattc
      361 gattttcatc tttcaaatcc accaagagat tcataacctc ctctgaattt tcaggatcaa
      421 gagctgctgt tggttcatct gccaaaatca agggtggatt tttcaaaata gttttcgcaa
      481 gggcaactcg ttgggcctct cccccagata aagtataaat tttttgttct aaatccaaat
      541 accctagatt aactttttct aaagccccca cttgcctttc caaacgttct acttttgaga
      601 ttttctgacc aacaaaaccc aaatccaaat tttctttgat tgattggttt tctaagaggc
      661 caaaattttg aaagagatag cccatctgat ctcgaaaata ctcacgagtg ggaatggttt
      721 ttaaatcttt cccctgatag agaacccttc ctccatctat cttttctagc tttcccaaga
      781 tattcagcag cgtcgtcttt ccgcttccac tctttccgat taaggcataa accttgccct
      841 tctccagctt gagattcaaa ccagagaaaa tcgcgcggtc attaaatttc ttttccaatc
      901 cttgaatatc aatcatctta ttttcctttc aatactgcca tgctaccagc ttcttctttt
      961 ttgtcctgcc ttactaaaat caagagggcg ttaagcgtaa acaaagctac aactagagcg
     1021 ctaatcaaac catctcttgt caaaatacta gataggacta ggccaagcaa gagaactcct
     1081 ccttgcgcca gtaaatactt gccatgaagc tcatagattg tcataccagc aagacgttta
     1141 atcataagct cccgtctgaa ctgctcaaaa tagagaagat tcatggaatc aaataacaag
     1201 atagccgtag acagggtcaa aatcgtccca ataaggagag agtaaaactc tacccgtttg
     1261 tcatttagta ccttggcaaa aaatagctgg ctttttacca agtaagaaac cttgtgatac
     1321 agaccttgct cttttagagc tgtgatgctg tctccatatc gatctagttt caaggcacta
     1381 ttagccgtag ttccccacaa catccctgca acaggtcttg ttccagtcgc ttgcggcgtt
     1441 agaactacaa taatcggatc ttttaggtac tggcgtgttg tcttgtaccc atcattatag
     1501 aggaaacgtt cctgtcctgt ttctgtaaaa gctagactta cctgtgggag gtagtatttc
     1561 tgactcgtca cttctacact ttcagatgaa aagttttgca ggtaatctgt aaacaatctt
     1621 tggtagtagg tagactgctc tcgcaagctc tcaggcaaga ttagcccaaa ctctccctca
     1681 gacaagtttt gcatcttgtc cataaactct gaagaaacgg taatcttttc ttctatcaga
     1741 tagcgtggtg agacatagat aacattccct gacggtgtgt agtcactgag acgattgcca
     1801 tctaggtcca cttctgctcc gtctgagaaa ttgtcaacat tgctcataat ataaaaagag
     1861 tctgtattgg ctaaccgttc ttcagtaaat gtctgccact cacgattatc cttacgcgtt
     1921 ccttcttcat cggcaaatgc actagaccaa ccaaaggata gacggtaacg gtctgaggac
     1981 tggctccatt tattgctagc tctctccatt tcctgcattt cacggtagtg gggtaggaga
     2041 gctgtcgcac tcgatccgac caccaataca gctaagagtt gccccaccat catcaatgtc
     2101 atcatacgtt tgagagggag tttccctttc aatagatcca ccagactatt ttcctgtaaa
     2161 ccaagtagat agacgacact tagtaaggta gaaatccctg ccagggtcaa tccataaagt
     2221 agaagagcaa tgatgaccag ttgcaccgtt gccataaaca aggcaccttg ataccagaga
     2281 atccccaatc ccaaaagact ggataccagc actgagcaga taaactgtct cacatcttct
     2341 aacactggtc tgagagcaac tccaaacaag ctctcaccag ctattaagcg aatccctgcc
     2401 tgacgaaggg atttgatccg ataaatcagg gtcagactca taaaggtcag cagaaaaata
     2461 gctaaactca gtagcaccat aggggtggcc gccaatagta agactatcga aaatggatct
     2521 tctccattcg aaacaaagcc ttgatagcca agttctttca aggtggtctg taagctcact
     2581 ccatccaaac ttcctgacac aatcaagtaa gaccctaata aatcactcgt ttctgcactc
     2641 tccttggagg aaatggtcag accttctgga agctctccct caccataagt ggcataggta
     2701 aagagtgttt ctccagcttc atttggctca acgattcgac gggcgatgag actattatgc
     2761 tctaccgcca agtgactcaa aacctctgtc acctcttcgc gactagctac tggttgattt
     2821 atcatcattc ctgtcacatc aacagaagga tagttaccaa ataaaatctc ttctcgatgg
     2881 tcaacggaag ttatcaccat ataaagcgat accaatacca ttgaaatcaa aataaataaa
     2941 cgtttcatca tttcctccaa aaagttactt ggtcacttag tgagaaggtg accacagtca
     3001 ctgcgatgtt aagaagctta ttaagttctt tctactagca acatcttcaa atacattata
     3061 tccgtttttt caagaactat caaggacatc aatgtcctta aaatctaact atttcatatt
     3121 atttttagct tatatctaaa aaaacagttc taaagacgaa ctttagaact gttttgatac
     3181 gaggacaagg acatttttgt cactgattca ctatgtttat aaatattttt tgacatcctc
     3241 ttgccattcc tctgtcaatt tctttactag aaatccatct ccaattagct tcgcaaatgt
     3301 tttagaagac tgataaaatg attcagctaa actaaaatct ctttcttctc ttaatgcttg
     3361 cttccacaaa aacataaata caagaggttt cttttgataa tcttgagttt tatccataat
     3421 ttcttgtaaa cacgatagat aatcattaaa acaatcagtt actcctacct tttcaagaca
     3481 acacattcct gaaataagag tatctctaat tataaagaag ttttctaatt tagttacctt
     3541 tttctgttcc aaaagaaaga gcattagctt ttcgatttga tttataaatt tggcaagatc
     3601 ttttctaatg agcatttgat aaaaaaacaa acgaatcaac aacatatcat ttgttgaata
     3661 aacttctttt tctatgatct gagacatatg ttcctcaagt attaactctg catattcagg
     3721 ccttctagtt ctagaagtat ccaaacacgc ctgcaatact tcacatgcta attgttcttc
     3781 tttaggaaga ttatcaaagt atgttttata aatctcttcc aaacacgctt ccttcttatc
     3841 gtactcctct tctttaccat agattggttc acgtaaaatc tggtatttca attctaaata
     3901 agcaggagga agagctgaaa aatccggcat aaggctataa actggaactc ctagccggcg
     3961 agcaatatag gctaacttgg gcaaactcgg ttgcgaagct ccactttcaa ttcgcgacag
     4021 ttgacgaact gtcagttctt gctcatctcc acaaaaatat ggacgactaa ctcctttttc
     4081 tatccgaatc tctttaattt tttgaccaat taacatcaga cgcctcctct tctacctgct
     4141 tcgaaaaata ctgtgaaact taaaaaattg taacggctac atctttccta ctcataagat
     4201 ttcatagctt cttccacttc tgccaaagta accccaaaca actctaggcg tttgaggagt
     4261 tgcttgccgt tagaatagcc gattcggaga gtctctccga gatattctct acgcttacgg
     4321 ctgtctgccc ctgctagaaa accaaggcga atcaagtcgc tacgactaat gtcaaactga
     4381 ctctcatgtt caaattgttc tgtcacctga gctagagccg ttttcaggtc ttcatagctg
     4441 gcatgctcaa ttcccagaga acgccccttg gtcttggact ttggaacagc ttcatctcgc
     4501 ttgagaaagg catgccgaac tgttggaatg gccgtcataa tcatgcgccg aatccgctcc
     4561 ccattaaaat ctgggtctgt aaagacaatg actccatgac gttggtgcag gcgctgaatc
     4621 cgctctatat cctgagcatt gatggcagaa cctcgagtct cataggtctc cacatcgaaa
     4681 taacgtttga gattgaccgt atcatcacgc ccttcaacca cgataacttg agaaattttc
     4741 tctttcatta cttgctgtcc aatccaaaaa tgcgttctgc atttgcagtc gttgctaccg
     4801 ccagctcttc tgtcgtcata ccacgcaagt cagcgataaa gtcgaccaca tagcgagtat
     4861 aggctgtttt attttcacga ccacgcttgg gtacaggtgc taagtaaggc gcatctgttt
     4921 ccaccaaaat cttgtccaaa ggtaactctt tagctgcttc ttggaggtca gttgccttct
     4981 taaaagtcac cactcctgag aaggaaatgg tcataccaag atccacaaac ttctctgccc
     5041 actcaagcgt ccctgaaaat gaatgcatga taccaccacg aggaccaacg ccctcactct
     5101 tgataatctc ataggtatct tccagcgcat cacgggtatg gacaacaaaa ggcaaatcca
     5161 agtccttaga tagctgaatc tgacggcgaa aaacctgctc ctgcacctct ttgggcgctg
     5221 tcatccaatg gtaatctaag ccaatttcac ctaaagccac aaccttggga tgttttaact
     5281 tatccaacaa gtaagcctca acttcctctg tataagtacc agcttctgta ggatgccaac
     5341 caatagtcgc atagagctga tcatactcat ctaccaactc caaggcacgc tcaatcgtcg
     5401 gtttatcaaa accaacaata ttcatctgtg tcacacccat ctcagcagcc aaggcaattt
     5461 cttctgcctc acgacctgca aattcttcta cattcaagtg tgtatgtgta tcaaaaatca
     5521 tctcttctaa cctcgttttc tatcttctat tataccaaaa aagaggaggg gcacctaatt
     5581 tttcggtttc ccctcctctc ttcaatagag agctattctg ctatcttttc tatccgatat
     5641 tgcccatctc ctattccaca gttagagaca gaagagattg gctacattga tagaatattc
     5701 ctattcagct aaagtatgtg attatcttgg gttctcactt ttctcaccca tcactaggat
     5761 tgatgacttc tcaaaatccc ccttataatc taactttaag tagagaatag aattttctag
     5821 ctgttctatt ttctacgtct agtctttctt cctagcaaag caaaaccact tgttgccacc
     5881 actagaccaa gactagcaag tgctgcactt gatgctgaac ctgtgttagg taattgtttg
     5941 ccagcaggtg actggtaggt aacatcttgc attgttcctg cagttgcttg cggtgcactt
     6001 gttgtcttag gagcagcatt aggagccact ggtctcttag gaactgtaac catgacaatg
     6061 gcttgcactc caatcgtttc tgcttccttc acatcttcca tagttttcgc attttcaatc
     6121 tctttgagag ctgcttgttt ttctgcttcc actcttgcta aaagttctgc tttttcttta
     6181 tcagaaagcg gtgcgccttt gatttcatcc tgcttgtcct tggctgcttt ttcaatggca
     6241 tttttagctg attctttttc agtagctaat tgcttagaag cttgtgattg agctgagttt
     6301 tgtgaacctg tgtgagctga tccacccgcc tggctaccac cgcctgcatt cgcattagca
     6361 tctgagcttc cagtattgcc tcccttatct ccacctggtg tattttctag cttatactct
     6421 ggtgtagctt cttgagttac agattcacct ttcgcagatt gttgaactgt atcttttcct
     6481 agaatcgtca ctactgaacc atctgggaat gtgattgttg ctgtaccatc tccagctaca
     6541 ttgattgtcg ctccgtctaa tgctgaacca tttgcttgta aaatagcaac cttaactttt
     6601 tcttcatctg ttaaatgagt tatatctttt acatctactc tttgagcagg tgctttaaca
     6661 gttgattttt cagcagtcgg tttagtgcta tcttgtgctt ttggatcttg tgctaccaat
     6721 tgatttcctg gaattgtaat agctgaacca tctttaaaga cgattgtcgc tgcgccatct
     6781 ccagctactt caactttata gtaaaatgaa ataataacag gacaaatcga tcaggacagt
     6841 caaatcgatt tctaacaatg ttttagaaat agaggtgtac tattctagtt tcaatctact
     6901 ataacatcgc ttccgaatcc atttgcatct actgaacttt tttacaaaaa taaaaatcag
     6961 tagactcgag ttcctactga tttttgatag aattcgtttg gattatgcag ccaaaacttt
     7021 gcgtccttta cgacgacgag ctgccaatac gcgacgacca tttttagttg acatacggtt
     7081 acggaatccg tgtttgcgcg cacgacgaag tttacttggt tgataagtac gtttcacgat
     7141 gaatacctcc tcatagattt tgtattcgtt tagccggcta taaagtgatc agttactaaa
     7201 catactttac tattctatct gattctttca cttttgtcaa tagctctagg caaatgtttc
     7261 cagctttttc gaatgcaagc cct
//
LOCUS       DAVBCW010000094         7115 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVBCW010000094 DAVBCW010000000
VERSION     DAVBCW010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7115
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.094"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            487..1530
                     /locus_tag="VQ514_001681"
     CDS             487..1530
                     /locus_tag="VQ514_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HET2500419.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGHNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFYTESEEQ"
     gene            1697..3784
                     /gene="topA"
                     /locus_tag="VQ514_001682"
     CDS             1697..3784
                     /gene="topA"
                     /locus_tag="VQ514_001682"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HET2500420.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNDEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQNGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            4005..4364
                     /locus_tag="VQ514_001683"
     CDS             4005..4364
                     /locus_tag="VQ514_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HET2500421.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            4457..5089
                     /locus_tag="VQ514_001684"
     CDS             4457..5089
                     /locus_tag="VQ514_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HET2500422.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDIRLTAQAGSQGVVFGTLTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLENRQTFIDQLGVTQLHGTKVVF"
     gene            5133..5789
                     /locus_tag="VQ514_001685"
     CDS             5133..5789
                     /locus_tag="VQ514_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HET2500423.1"
                     /translation="MFEIFKSYQFNQEKAHAFGFVVNSGVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETDDLYPHVHMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            5808..6440
                     /locus_tag="VQ514_001686"
     CDS             5808..6440
                     /locus_tag="VQ514_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HET2500424.1"
                     /translation="MNFQLAKYSLLKKFSENIEFTTPGEYGAIFKYLIENVKTDRQII
                     CSPHCHDDLGMAVANSLAAVKNGAGRVEGTINGIRERAENAALEEIAVALNIRQDYYQ
                     VETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
                     TPELVGVKIPLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALADKK"
     gene            6468..6902
                     /locus_tag="VQ514_001687"
     CDS             6468..6902
                     /locus_tag="VQ514_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HET2500425.1"
                     /translation="MVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEF
                     NATGQGSVEAIFNAIDKFFNQSVRLVSYTINAVTDGIDAQDRVLVTVENRDTETIFNA
                     AGLDFDVLKASAIAYINANTFVQKENACEMGRSVSYHDMPSV"
     gene            6914..>7115
                     /locus_tag="VQ514_001688"
     CDS             6914..>7115
                     /locus_tag="VQ514_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HET2500426.1"
                     /translation="MAKKIVALVGDGIGPEIMEAGLEVLEALAEKTGFDYEIDRRPFG
                     GADIDAAGPPLPDETLKASREAD"
BASE COUNT         2201 a         1233 c         1569 g         2112 t
ORIGIN      
        1 aaggtgcact gttatagatt caatacacta taatttagtg taattgagaa aggagaaatg
       61 attgtgattg atgttggcta ggttatgttc aatgattcct accgtctcaa atcttgtcag
      121 taaggaaaaa taaattcttc aaaagtagag attacaaggc ttgtttaaga aagaattcaa
      181 agaccttgac aaataaaaat aaaatggtta ttataaaaaa tggtctgaaa tagatgatga
      241 tacttttcga aaatcttttt acgtcagctc agctttgcct tgctgtgttt tgagcaagct
      301 acggttagct tccgagtttg attttcattt actagaaatg aaactgatga gagatatcag
      361 tagacatttg agtcaggata ttatggaaaa tgataaaaag agctcgtgag attggcatat
      421 cagactacta aagtattgag tttgttagga ttttagcgac tagttagctg ggaaaggaag
      481 atatttgtga caaataataa actgtattcg ttgatagaat ttagaaataa aatatatgaa
      541 gaattagaac tttccagaag tgatttagcg attttactat gtgccatgct tatcgcctct
      601 atcggattaa atatggattc gactcccgtg attattggag ccatgttaat ctctcctttg
      661 atgacaccta ttctgggagt ggggctctct ctagctatat ttgattttaa attgttaaga
      721 aaatctttta aaatattagc tattcaaatt cttgccagtt taatagcttc aacactttat
      781 ttttatcttt ctcccatttc gtatgctagt tcggaaattg ttgctagaac ctctccgact
      841 atttgggatg ttctcattgc ttttgtagga gggatagcag gtattattgg tgctaggaaa
      901 aaagagacca ataatattgt tcctggtgtt gctattgcaa ccgccttgat gcctcctctt
      961 tgtacagtag gttatgctat tgcttctgct aatctaaaat ttatcatagg ctcctcttac
     1021 ctattcctca tcaattgtag ctttattgtc attgcgactt atataggtgt taggttgatg
     1081 atggttaaga aacattattt taaagataat gaagaagact ctaaaatgcg taggattttg
     1141 cttctagttg ctgttttgct gatgattccg agtttcatct ctgcaacgac tttagtgaga
     1201 gaaacgttga aaaaagagtc ccttaagaaa tttatatcag agcagtttca ggggcataat
     1261 attttgaaaa aaacctattc taaaaagact cataccctaa agctaaccat ttcaggaaat
     1321 tatttgacag aagaagaact cgatatgatt tcaagtaaga gaggtgacta tggtttaagt
     1381 gatgtttctg ttcaagtttc acaattgtct gattcagaac aacttagtaa ggaagaactg
     1441 gtggagtatt tcttccagta tatcaaggat aaggaagcaa aagaaaagga aaaagctaat
     1501 aagttttata cagagtctga ggagcaataa tttcttgaga atagctggtt tctctcgtga
     1561 gtcttctatg tatatcaaag gaagactgag gttttaagta taaaactttt cttctattat
     1621 agtagctaag tcttgacagg gttcaaaaaa tggtttacac tttataaagt ttattacttt
     1681 gaaaaggtgt gatactgtgg ctacggcaac aaaaaagaaa aaatcaacag ttaaaaaaaa
     1741 tctagtcatc gtggagtcgc ctgctaaggc caagacgatt gaaaaatatc taggcagaaa
     1801 ctacaaggtt ttagccagtg tcgggcatat ccgtgatttg aagaaatcca gtatgtccgt
     1861 cgatattgaa aataattatg aaccgcaata tatcaatatc cgaggaaaag gccctcttat
     1921 caatgacttg aaaaaagaag ctaaaaaagc taataaagtt tttctcgcga gtgacccgga
     1981 ccgtgaagga gaagcgattt cttggcattt ggcccatatt ctcaacttgg atgaaaatga
     2041 tgccaaccgt gtggtcttca atgaaatcac caaggatgca gtcaaaaatg cttttaaaga
     2101 acctcgtaag atcgatatgg acttggtcga tgcccaacaa gctcgtcgga tcttggatcg
     2161 cttggtaggg tattcgattt cgcctatttt gtggaagaag gtcaagaagg gcttgtcagc
     2221 aggtcgcgtt cagtccattg cccttaaact catcattgac cgtgaaaatg aaatcaatgc
     2281 cttccagcca gaagaatact ggacagttga tgctgtcttt aaaaagggaa ccaaacaatt
     2341 tcatgcttcc ttctatggag tagatggtaa aaagatgaaa ctgaccagca atgacgaagt
     2401 caaggaagtc ttgtctcgtc tgactagtaa agacttttca gtagatcagg tggataagaa
     2461 agagcgcaag cgcaatgctc ctttacccta taccacttca tctatgcaga tggatgctgc
     2521 caataaaatc aatttccgta ctcgaaaaac catgatggtt gcccaacagc tctatgaagg
     2581 aattaatatt ggttctggtg ttcaaggttt gattacctat atgcgtaccg attcgactcg
     2641 tatcagtcct gtagcgcaaa atgaggcggc aagcttcatt acggatcgtt ttggtagcaa
     2701 gtattctaag cacggtagca aggtcaaaaa cgcatcaggt gctcaggatg cccatgaggc
     2761 tattcgtccg tcaagtgtct ttaatacacc agaaagcatc gctaagtatc tggacaagga
     2821 tcagctcaag ctatataccc ttatctggaa tcgttttgtg gctagccaga tgacagcggc
     2881 cgtttttgat accatggctg ttaaattgtc tcaaaatgga gttcaatttg ctgccaatgg
     2941 tagtcaggtt aagtttgatg gttatcttgc catttataat gattctgaca agaataagat
     3001 gttaccggac atggttgttg gagatgtggt caaacaggtc aatagcaaac cagagcaaca
     3061 tttcacccaa ccgcctgccc gttattctga agcaacactg attaaaacct tagaggaaaa
     3121 tggggttgga cgtccatcaa cctatgcgcc aaccattgaa accattcaga aacgttatta
     3181 tgttcgcctg gcagccaaac gttttgaacc gacagagttg ggagaaattg tcaataagct
     3241 catcgttgaa tatttcccag atatcgtaaa cgtgaccttc acagctgaaa tggaaggtaa
     3301 actggatgat gtcgaagttg gaaaagagca gtggcgacgg gtcattgatg ctttttacaa
     3361 accattctct aaagaagttg ccaaggctga agaagaaatg gaaaaaatcc agattaagga
     3421 tgaaccagct ggatttgact gtgaagtgtg tggcagtcca atggtcatta aacttggtcg
     3481 ttttggtaaa ttctacgctt gtagcaattt cccagattgc cgtcataccc aagcaatcgt
     3541 gaaagagatt ggtgttgagt gtccaagctg tcatcaggga caaattattg agcgaaaaac
     3601 caagcgtaat cgcctattct atggttgcaa tcgctatcca gaatgtgaat ttacctcttg
     3661 ggacaagcct gttggtcgtg actgtccaaa atgtggcaac ttcctcatgg agaaaaaagt
     3721 ccgtggtggt ggcaagcagg ttgtttgtag caaaggcgac tacgaggaag aaaagattaa
     3781 ataagaggag gggcccgaaa gtaaatttca ggctcctttt ggttagaact tgacaaaata
     3841 catcatttta tgcgaaacta gaagaagatt attttatact caatgagaat caaagagcaa
     3901 attagaaagc tagtcgcagg ttgctcaaag cactactttg aggttgaaga tggaagctga
     3961 cctggtttga agagattttc gaagagtatt aactaggaga agttatgcgt cttatctatc
     4021 tgataattgg ttttttatca ctggccttgg ctattgttgg ggttgtttta cccttgttgc
     4081 cgacaacgcc tttccttttg ttgtctattg cttgtttctc cagaagttcc aagcgtttcg
     4141 aagactggct ttatcatacc aagctctatc aaacatatgt agctgatttt cgcgagacta
     4201 agtctattac gcgtgaacga aagaaaaaaa tcatcgtctc tatctacgtc ttgatgggaa
     4261 tttctattta ttttgcacct cttttaccag tcaaaatcgg tctgggtgct ttgaccatct
     4321 ttattactta ttatctcttc aaggtcattc cagacaaaga atagttaaaa cagtagttat
     4381 ttgccttgat aaaattgaaa gcatattcat aacaatatga tataataaat ttgaagtaat
     4441 attcaaggag aatcaaatga tttacgaatt ttgtgctgaa aatgtgactt tacttgaaaa
     4501 agcgatgcag gctggagctc gtcggattga actctgtgat aatctagcag ttggtgggac
     4561 aacacccagc tatggagtga ctaaggcagc ggttgaactg gcagctaact acgatacaac
     4621 catcatgacc atgattcggc cacgtggtgg tgactttgtc tataatgacc tagaaattgc
     4681 tatcatgcta gaagacattc gtttgactgc tcaggctgga agtcaagggg ttgtatttgg
     4741 aactttaact gctgataaaa agttggataa gcctaatctg gaaaagttaa ttgctgcatc
     4801 aaaaggaatg gaaattgtct ttcacatggc ctttgatgaa ctaagtgatg aagatcaacc
     4861 ggaagctatt gactggctca gtcaagccgg tgtcactcgt atcctaactc gtgctggtgt
     4921 gtctggcgac tccttagaaa aacgttttgt tcactatcac agaattttgg agtacgctaa
     4981 aggtaaaatt gaaattctac caggtggggg gattgacctt gaaaaccgtc aaacctttat
     5041 cgaccagttg ggggtaacac aattgcatgg tactaaggtt gttttttaaa aaatagaaag
     5101 gaactgctag ctttgggtag cagttttcac ttatgtttga aatttttaaa tcctatcaat
     5161 ttaatcaaga aaaggctcat gcttttggtt ttgtagtaaa tagtggagtc tggacctata
     5221 gttgccagat tttgcaaggt gactttgtca tgactgtgtc catcactgct gataatgtga
     5281 actttcaagt ctttgaccaa gagactgatg acctctatcc tcacgttcat atggaaagca
     5341 tgaggggaag ttttgtcgga aatgtccgtg aggcttgtct ggagattctt taccagattc
     5401 ggaaggcttg ttttgatgtg caagatttta tctgtcatca gactaagcgt attatgactc
     5461 aagttcagga aaagtatgga aaccagttgg agtatctgtg ggaaaaatcg cctgatacag
     5521 ctgtattgcg ccatgaaggc aatcaaaagt ggtatgccgt cttgatgaaa atctcttgga
     5581 ataagctgga aaagggcaga gaaggacaag tggaagcagt caacctcaag catgaccaag
     5641 tagctaattt actttcacaa aaggggattt atccagcctt tcatatgagc aagcgctact
     5701 ggattagtgt gtcccttgat gatactttat cagatgaaga agtactggaa ttgatagaaa
     5761 aaagttggaa cttaacctct aaaaaatgaa atattttaat aattttcatg aactttcaat
     5821 tagctaaata ttctttactg aagaaatttt cagaaaatat agaatttacc acaccagggg
     5881 aatatggtgc catcttcaaa tacctgattg agaatgtcaa gacggatcgt cagatcatct
     5941 gttcgcctca ctgtcatgat gacctcggaa tggcagtggc aaatagcctt gctgctgtca
     6001 agaatggtgc aggacgtgtt gaagggacta tcaatggtat tagggagcga gctgaaaatg
     6061 ctgctttgga agaaattgca gtggctctca atattcgcca agattactac caagtagaaa
     6121 ccagtattgt cctaaatgag accatcaata cgtcagaaat ggtttctcgc ttctctggta
     6181 ttccagttcc taaaaacaaa gccgtcgttg gtggcaatac cttctcccat gaatctggta
     6241 ttcaccaaga tggagtcctt aaaaatcctc tcacttatga gatcatcaca cctgaattgg
     6301 ttggtgttaa gattccgctt ggaaaattat ctggtcgcca tgcttttgtt gagaaactga
     6361 gagaattggc cctagatttt acagaagagg atatcaaacc actctttgct aagttcaagg
     6421 cactggccga taagaagtaa gaaatcacag atgcagatac tcgagctttg gtagctggaa
     6481 ccatggttga aaatccagaa ggcttccact ttgatgattt acaacttcaa actcatgcag
     6541 ataatgacat tgaagcgctc gttagcctag ccaatatgga tggtgagaaa gtcgaattta
     6601 atgcgacagg gcaaggttcc gttgaagcaa tctttaacgc tatcgataag ttctttaacc
     6661 aatctgttcg tttggtgtcc tacactatca atgcggtaac agatggaatc gatgcccagg
     6721 atcgggtttt ggtcactgtt gaaaacagag atacagaaac catctttaat gcagcagggc
     6781 ttgattttga tgtgttgaag gcttctgcta ttgcctatat aaacgctaat acctttgttc
     6841 aaaaagagaa tgcatgtgag atgggacgca gtgtttctta ccacgatatg cctagtgtgt
     6901 aaaggagaag gctatggcaa agaaaatagt agctctagta ggagacggaa ttggcccaga
     6961 aatcatggag gctggtttag aagttctgga ggctctagct gaaaaaacag gttttgacta
     7021 tgagattgac agacgaccgt tcggaggtgc agatattgat gcagcaggac ctcccttacc
     7081 tgatgaaacc cttaaggcaa gtagggaagc agatg
//
LOCUS       DAVBCW010000095         7061 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVBCW010000095 DAVBCW010000000
VERSION     DAVBCW010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7061
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.095"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(52..1113)
                     /locus_tag="VQ514_001689"
     CDS             complement(52..1113)
                     /locus_tag="VQ514_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HET2500427.1"
                     /translation="MNKRVQAFLAKMQEKELDGVIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMGLTRIGFEDEISVSYY
                     HRMQAAFAGLNLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            complement(1106..3937)
                     /gene="uvrA"
                     /locus_tag="VQ514_001690"
     CDS             complement(1106..3937)
                     /gene="uvrA"
                     /locus_tag="VQ514_001690"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET2500428.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAIKASSVEQIVDKVLELPERQRLQILSPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDNSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPNASKTLHEGALAPWNPISSNYYPNMLEQAMTAFGVAMDKP
                     FEDLSEEDKNLILYGSDGKKFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCHGYRLNDQALSVRVGGEQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVIGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            4069..5013
                     /locus_tag="VQ514_001691"
     CDS             4069..5013
                     /locus_tag="VQ514_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HET2500429.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            5025..5708
                     /locus_tag="VQ514_001692"
     CDS             5025..5708
                     /locus_tag="VQ514_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HET2500430.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASVFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            5963..6994
                     /locus_tag="VQ514_001693"
     CDS             5963..6994
                     /locus_tag="VQ514_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HET2500431.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGRALFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNAGHATPRAIVPFGVHAHVDAQE
                     QVIRFDYNK"
BASE COUNT         1990 a         1666 c         1437 g         1968 t
ORIGIN      
        1 aatttttttt gacttttttc gtcattttcg tacttttttc ttgacaaaca actaaatgac
       61 tatcaactct tttggagcta gggtcaataa ttcacaacct gtctctgtaa tcaggatatc
      121 atcctcgata cgaacgccat atttgccttc gatatagata cctggttcat cggtcaaggc
      181 catacctgtc ttaatagttt ctgtagaagt ctgactaaag tagggttcct catggatatc
      241 cagaccaata ccgtggccaa tgccgtgagt aaagtagtca ccataacctg cctcaatgat
      301 aatatcacga gggattttgt caaagtcacg gaaacctaag cctgccttag cttggtcaat
      361 caaggcttgg ttagctttta gaaccgtatt gtaaatctct gcctgctcat cgctaacatg
      421 ccctagatag atagtccggg tcatatcact gacatagtgg tcatagagac agccgaagtc
      481 catggtaatg gcttctccca actccactgg tttgtgcatt ggatgggcat ggggtttaga
      541 agaattgata ccgctagcta ggatcgtatc aaaagataag ccagatgctc ccaactcacg
      601 catgcggaaa tcaaggaagt tggcaatctc aatttcagtt tttcctggtt tgataaagtc
      661 aagcgcatcg cggaaagctt ggtctgagat agaacaagcc ttgcgaatcg ctgcaatctc
      721 tgcctcatcc ttaatcatac gaagaccttc cacaaactga gtttgtggaa gcaagttcaa
      781 acctgcaaaa gctgcctgca tacggtggta ataagacact gaaatctcat cttcaaaacc
      841 gatacgagtc aagcccatgt ccttaacaat tcctgcaatg acagccaatt catcacgatc
      901 agccacaatc tcaaaaccac tggtttcttg cttagctgcg atgatatagc gagagtctgt
      961 cactaatacc tgacggtcac ggctgataaa gactgttccg tttgagcccc aaaaaccagt
     1021 caaataatag acgtttttaa gattgttgat gatgacacca tctagttctt tttcttgcat
     1081 tttagctaga aatgcttgta cgcgtttatt catgatgtaa ctttcctttc aaatagtgtc
     1141 ctgtatagct ggcttcgttg gcagctactt cttctggagt tcctgttacg atgatggttc
     1201 caccaccgac accgccctca ggtcccaagt caatgatatg gtctgccgtc ttgataacat
     1261 ccagattgtg ctcgatgacg aggactgtat tgccatcgtc tacaaagcga gctaaaacct
     1321 tgagcaggcg agcaatgtcc tctgtatgaa gccctgtcgt cggttcatcc agaatgtaga
     1381 aagattttcc tgtcgagcgt ttgtggagtt cgctagctaa cttcatacgt tgggcttctc
     1441 ccccagaaag ggtggtagct ggctgtccca aggtcacata gcctagccct acatccttga
     1501 tggtctggag tttgcgttga attttcggaa tgtgttggaa aaattctacc gcatcgttga
     1561 ccgtcatatc caagacctgc gaaatattct tttccttgta gtgaacttct agggtttcac
     1621 tgttatagcg ggttccgtgg caaacttcac aagccacata aacatctggc aagaagtgca
     1681 tctcaatctt gataatcccg tcacctgagc aagcttcaca gcgacctccc ttgacgttga
     1741 aactgaagcg tcccttcttg tagcctcgaa tcttggcttc atttgtctga gcaaaaaggt
     1801 cacgtatatc gtcaaaaact cctgtatagg tagctgggtt agacctcggc gtccgtccga
     1861 tagggctctg gtcaatatca atcaaacggt cgacatgctc aatccctgta atagtcttaa
     1921 acttaccagg tttgtctgaa ttacggttga gcttctgggc aatggctttt ttgagaatgc
     1981 tgttgattag agtcgatttc cctgaacccg acacacctgt cactgcgata aattttccta
     2041 gtggaaagcg agccgtgaca ttttgcaagt tgttctcacg cgctcctatc acttcaataa
     2101 aacgaccatt tccgacacgg cgctcttctg gtactgggat gacacgtttg cctgacaagt
     2161 actgacctgt gatagacttg ctgttgcgag ccacttgctt aggtgtacct gctgcaacaa
     2221 tctcaccacc aaaaacaccg gcaccaggac caacgtcaat cagataatca gcttcacgca
     2281 tggtatcttc gtcgtgttcc accacgataa gagtattgcc caagtcacgc atctttttca
     2341 gactggcaat caggcggtca ttgtctctct ggtgaagacc gattgacggc tcgtctagga
     2401 tatagaggac gcctgatagg ttggaaccaa tctgggttgc caaacgaatg cgctgacttt
     2461 ccccacctga aagggttcct gctgaacgtg acagggttag atagttaaga cccacattat
     2521 taaggaaggt caaacgatcc ttgatttcct tgagaatggg acgagcaatg atggcttcat
     2581 tttcagacaa agttaactgg ctcaccaagt ccaagtgatc tgcgatagac aggtctgaaa
     2641 tttctccgat atgtggccct tgctcaccac ccacacggac agacaaggcc tggtcattga
     2701 gacgataacc gtgacaggtt ccgcaagtca gctcattcat gtagaggcgc atctgagtgc
     2761 gagtgtaatc gctattggtt tcatggtaac gacgtttgat attattgata actccctcaa
     2821 acggaatgtc gatatcgcgc acgccaccaa attcattctc atagtggaaa tggaatttct
     2881 taccatctga gccgtagaga atcaagttct tgtcttcttc tgacaagtcc tcaaaaggct
     2941 tatccatagc cactccaaaa gccgtcatgg cctgctctag catgtttgga tagtagttgg
     3001 atgagatagg attccaaggt gccagcgctc cctcatgtaa ggttttgctg gcatttggca
     3061 ctaccaaatc agtatccacc tccagcttga tgcccaagcc gtcacactca ctacaagagc
     3121 caaaaggagc attgaaagaa aagagacgag gctctaactc tgggacagta aaaccacaaa
     3181 ctggacaggc ataatgctca gagaacaaca actcagagtt gtccatggtg tcgataatga
     3241 cataaccttc tgcaatacga agggcagcct caatggaatc aaagagacga ctacgaatgc
     3301 cctccttgat aacaatacgg tcaaccacga catcgatatt gtgttgcttg ctcttagaca
     3361 actctggcac ttcggtcaca tcatagactt ccccatccac acggacacga acatacccgt
     3421 ctttctgaac cttctcgata acactcttat gttggccttt tttcttgcgg atgacaggag
     3481 acaagatctg caagcgctgg cgttcaggta actccaaaac cttatcaacg atttgctcca
     3541 cagaagaagc cttgatagcc ccatgcccgt tgatacagta aggcgtcccc acacgcgcgt
     3601 agaggagacg cagatagtca ttgatttcag tcgttgttcc caccgtcgag cgagggtttt
     3661 tgctagtcgt tttctggtcg atagaaatag ctgggctgag accatcaatg gcatctacat
     3721 ctggtttttc catatttccc aagaactgac gagcgtaggc ggacaaactc tctacatagc
     3781 gacgttgtcc ctccgcatag agggtatcaa aagccaaact ggacttccct gaacctgaca
     3841 agccagtcac gacaaccaac ttgtctcgcg gaatctccac atcaatattt tttaaattat
     3901 gggcacgcgc cccatgaatg acaattttat cttgcatctt tgttctttct agtccattat
     3961 tgcttaccat tataccaaaa aaagtgagat tctattaccc aaaaggccga ttttgtagta
     4021 taatagtaca gtgtgaaaaa atctgaaaaa tgagaaagga taagggatat gaaacaagtt
     4081 tttctctcta caacaactga atttaaagag atcgatacgc ttgaaccggg tacttggatc
     4141 aatctcgtca atccgactca aaatgaatca ctcgaaatcg ccaacacctt cgatattgat
     4201 attgccgacc ttcgagcacc gctcgatgcg gaagaaatgt ctcgtattac cattgaagac
     4261 gagtataccc tgattatcgt agacgtgccg gtcacggagg aaagaaataa ccgcacctac
     4321 tacgtaacca tcccgcttgg tattatcatc actgaggaaa ccattatcac tacgtgtttg
     4381 gaaccactac ctgtccttga tgtctttatc aaccgtcgat tgcgtaattt ctataccttc
     4441 atgcgttcac gttttatctt tcaaattctt tatcgcaatg cagagcttta cctaacagcc
     4501 cttcgttcaa tcgaccgcaa gagtgaacaa atcgaaagtc aactgcatca atcaactcgt
     4561 aatgaagaat tgattgagct catggaattg gaaaaaacta tcgtctattt caaggcctcc
     4621 ctcaaaacaa atgagcgcgt gattaagaaa ttgaccagtt caaccagcaa tatcaagaaa
     4681 taccttgagg acgaagacct gcttgaagac accctgattg aaacccaaca ggccatcgag
     4741 atggcagata tttatggaaa cgtcttgcat tctatgacag agacctttgc ctctatcatt
     4801 tctaacaacc agaacaacat catgaaaacc ttggcccttg tgaccatcgt catgtccatc
     4861 ccaaccatgg tcttttctgc ctacgggatg aactttaagg ataatgaaat tcccctaaac
     4921 ggagagccaa atgccttctg gttaatcgtc tttatcgcct ttgctatgag tgtctcgctc
     4981 actctctatc tcatccataa aaaatggttc taaaaggagt tcctatgtct caaattgatc
     5041 tacaaaaatt aactaagaaa aaccaagagt ttgtccacat tgctacccaa caattcatca
     5101 aagatgggaa aacagacgct gaaatccaga ctatttttga ggaagtcatt ccccaaatcc
     5161 ttgaggagca atctaaaggt acaactgccc gttccctata cggcgcacca actcattggg
     5221 ctcatagctt cactgtcaaa gagcagtacg aaaaagagca tccaaaagaa aatgatgacc
     5281 caaaactgat gattatggac tcagctcttt tcatcactag cctctttgcc cttgtcagcg
     5341 ccctcacaac cttctttgcg gcagatcaag ctttcggcta tggattgatt actcttctat
     5401 tagttggact ggttggtgga tttgccttct acttgatgta ctactttgtt taccaatact
     5461 atggaccaga tatggatcgc agtcaacgtc cacctttctg gaaatctgta ctagttatcc
     5521 tagcttctgt gttcctttgg ttgcttgtct tctttgcaac aagcttccta ccagctagcc
     5581 ttaacccagt actggatcca ttgccactag ctattattgg agcagccctc ctagcccttc
     5641 gcttctatct caagaaacgc ttgaatatcc gtagtgcaag tgcaggacca acacgctatc
     5701 aagaataaga aaacgataaa agcaactgca ggtgcggttg ctttttcact tacttttttg
     5761 agttatactc aatgaaaatc aaagagcaaa ctaggaagct agctgcaggt tgctcaaagc
     5821 acagctttga ggttgcagat aaaactgacg tggtttgaag agattttcga agagtattaa
     5881 aagtattctt ctgaaatccc acatagcttt ctcttatatt ttgtgataaa ataggctcaa
     5941 tctatttcta ggaggatgag atatggtttc tactattggt attgttagtt tatctagtgg
     6001 cattatcgga gaggattttg tcaaacacga agtggacttg ggtatccaac gtctcaagga
     6061 tctgggactc aatcccatct ttttgcccca ttcgttaaaa ggattagact ttatcaagga
     6121 ccatcctgaa gctcgtgcag aggatttgat tcatgccttt tctgatgata gcatcgacat
     6181 gatcctatgt gccatcggtg gagacgatac ctatcgcttg ctaccttatc tttttgaaaa
     6241 tgaccaactc caaaaggtta tcaagcaaaa aatttttctt ggcttctcgg atacaaccat
     6301 gaaccacctc atgttgcata aattaggaat caagactttt tatggtcaat cctttttagc
     6361 agacatttgt gaattagaca aagaaatgct agcctatagc cttcactact ttaaagaatt
     6421 gattgagacg ggaagaattt cagaaatccg ccctagtgac gtttggtatg aggaacgaac
     6481 tgattttagt cccacggccc tgggaacacc tcgtgtcagt catacaaata caggttttga
     6541 cttgttgcaa ggaagggcac tattcgaggg gaaaatcctc ggtggttgcc tcgaatccct
     6601 ctacgatatc tttgacaact ctcgatacgc agatagcacg gagctctgcc aaaaatacaa
     6661 acttttccct gacttgtcag actgggaagg gaaaatcctc ttgctcgaaa caagcgaaga
     6721 aaagcctaag ccagaagact tcaaaaagat gttgctaact ttaaaggaca ctggcatatt
     6781 cgcggtcatc aatggactct tggtcggaaa acctatggat gaaactttcc atgacgacta
     6841 taaagaggca ctattggata tcattgacag caatatcccg attgtctata atctgaatgc
     6901 cggccacgca actccaagag caattgtacc ctttggggtc catgctcatg tagatgcaca
     6961 ggaacaagtc attcgctttg actataacaa ataaagctgg gaaaagtttc tcagcttttt
     7021 tctcggtatc attactggca ttttcatcac atattacagc a
//
LOCUS       DAVBCW010000096         7013 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVBCW010000096 DAVBCW010000000
VERSION     DAVBCW010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7013
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.096"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(317..2008)
                     /locus_tag="VQ514_001694"
     CDS             complement(317..2008)
                     /locus_tag="VQ514_001694"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HET2500432.1"
                     /translation="MNTKELIASELASIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            2144..2590
                     /gene="argR"
                     /locus_tag="VQ514_001695"
     CDS             2144..2590
                     /gene="argR"
                     /locus_tag="VQ514_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET2500433.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            2641..5175
                     /gene="mutS"
                     /locus_tag="VQ514_001696"
     CDS             2641..5175
                     /gene="mutS"
                     /locus_tag="VQ514_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HET2500434.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKLDSQNNFLVAIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSCQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPALAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLITGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTENPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            5322..>7013
                     /locus_tag="VQ514_001697"
     CDS             5322..>7013
                     /locus_tag="VQ514_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2500435.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDVPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQEGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
BASE COUNT         1901 a         1370 c         1667 g         2075 t
ORIGIN      
        1 tttggttagg tgaattagtg aagcgtttag gcaagtgtct ctggttacga cgtcatggac
       61 tctaaatcga ttatatttag gggtcatgac tagtgaagca gttagctagt tcgcatataa
      121 gcggctagcg tctaacaatt aggaacttta gttccaataa ctttaagatt acgacgtttt
      181 aggacataaa tcgatcatat ttatgtccta aaactagtga agtatgtcct aaaactagtg
      241 aagcgcctag ccaaagtccg aataggattt ggcgttagtt acttagattg ctttgcaatc
      301 aagtaacttt ggcgatttac atcttctctg gcgcttctac tccaagcaag cgaagggctt
      361 ctttgagaac gactgcggtt gcgtagctga gggctagacg gctgtcgcgt tctgggcttt
      421 catccaagat acgtgtatgt gcatagtatt tgttaaagga ttgagccagg ctaattgcaa
      481 atttagcaat gatagaaggt tcaaagttat ctgccgcacg gttgataata cgtgggaagt
      541 cttgaatgag tttaatgatt tcccagcttt cagtatcatt caagctatag ttgccagctg
      601 tttctggttt gaaatcggct ttgcgtaaga tagattggat acgagcgtag gcatattgaa
      661 cgtaaggtcc agtttcaccc tcgaaggata ccatagcctc taggtcgaag tcgtatccat
      721 ttgtacggtc ggttttgagg tcatagaatt taatggctcc aaccccaaca gcatgtgcta
      781 cttggtcttt gttttctagt tcaggatttt tagcctcgat ttggaccttg gcacggctaa
      841 cagcctctgc aacagtaggc tctagcaaga tgacattccc tttacgagta gagagtttct
      901 tcccttcttt tgtaaccaaa ccaaaaggaa cgtgagtaat gtcgtcactc cagtcgtagc
      961 ccatctcttg caagacagct ttgagctgtt taaagtgggc agattgttct tgaccaacga
     1021 catagataga tttagcaaat tggtattcgt ttttacggta gagggctgca gccaagtcac
     1081 gtgtgatata gagagttgca ccatcagact tcttgatgag ggctggatgt tcaattccgt
     1141 atttctcaag attcacaact tgggcacctt ctgattcaag aagtagtcct ttttcagaaa
     1201 gaatgtctac aactgcatcc atcttatcat tgtagaaggc ttctccgtta tagctgtcaa
     1261 attcaacctt caattcattg taaaggcggt taaattccac taaactttca tcgcggaacc
     1321 attgccaaag agcgagagct tcctcatctc cattttcaag tttacggaac cattcgcgcg
     1381 cttcttcatc caagctaggg tcattttcag cttcagcgtt gatgcggaca tagagtttaa
     1441 ggagttcatc gattggatga gcttttacag cttcttcgtc gccccatttt ttgtaggcaa
     1501 caatcaacat cccaaattgt ttaccccagt ctcccaaatg gttgaccttg accgtttgat
     1561 aaccgatttt ttggaaaata tgtgacaagc tatctccgat aacagttgaa cgcaggtggc
     1621 caatagaaaa tggtttagcg atattcggac tagacatgtc gataacaaca ttttcttgtt
     1681 taccaatatt ttggtcagca tagtgttctt tttcagtggt aacagcttgc aatacttgag
     1741 cagaaatggc agatttatca aggaaaaagt taacgtaagg tcctgttgcg acaacttttt
     1801 caaaggcttg gctgttcatt ttttcagcca gttcagccgc aatcatttgt ggtgctttac
     1861 gttcgacttt ggcaagagaa aaagcaggga aagcgatgtc acccatttct gagtttttag
     1921 gggtttccag taactttaaa atagcctctt ggtccaggct atcaatgatg ctagccaatt
     1981 cgctagcaat caattctttt gtattcatta agagctcctt tttggacttt tctactattt
     2041 tatcacaatt ttaaagaaag aagaaaaaat ttttgaaatc tcctgttttt ttggtataat
     2101 atggttataa atatagttat aaatatgcac gcaagaggat tttatgagaa aaagagatcg
     2161 tcatcagtta ataaaaaaaa tgattactga ggagaaatta agtacacaaa aagaaattca
     2221 agatcggttg gaggcgcaca atgtttgtgt gacgcagaca accttgtctc gtgatttgcg
     2281 cgaaatcggc ttgaccaagg tcaagaaaaa tgatatggtg tattatgtac tagtaaatga
     2341 gacagaaaag attgatttgg tggaattttt gtctcatcat ttagaaggtg ttgcaagagc
     2401 agagtttacc ttggtgcttc ataccaaatt gggagaagcc tctgttttgg caaatattgt
     2461 agatgtaaat aaggatgaat ggattttagg aacagttgct ggtgccaata ccttattggt
     2521 tatttgtcga gatcagcacg ttgccaaact catggaagat cgtttgctag atttgatgaa
     2581 agataagtaa ggtcttggga gttgctctca agacttattt ttgaaaagga gagacagaaa
     2641 atggcgatag aaaagctatc acccggcatg caacagtatg tggatattaa aaagcaatat
     2701 ccagatgctt ttttgctctt tcggatgggt gatttttatg aattatttta tgaggatgcg
     2761 gtcaatgctg cgcagattct ggaaatttcc ttaacgagtc gcaacaagaa tgccgacaat
     2821 ccgatcccta tggcgggtgt tccctatcat tctgcccaac agtatatcga tgtcttgatt
     2881 gagcagggtt ataaggtggc tatcgcagag cagatggaag atcctaaaca agcagttggg
     2941 gttgttaaac gagaggttgt tcaggtcatt acgccaggga cagtggtcga tagcagtaag
     3001 ctggacagtc agaataattt tttggttgcc atagaccgcg aaggcaatca atttggccta
     3061 gcttatatgg atttggtgac gggtgacttt tatgtgacag gtcttttgga tttcacgctg
     3121 gtttgtgggg aaatccgtaa cctcaaggct cgagaagtgg tgttgggtta tgacttgtct
     3181 gaggaagaag aacaaatcct cagctgccag atgaatctgg tactctctta tgaaaaagaa
     3241 agctttgaag accttcattt attggatttg cgattggcaa cggtggagca aacggcatct
     3301 agtaagctgc tccagtatgt tcatcggact cagatgaggg aattgaacca cctcaaacct
     3361 gttatccgct acgaaattaa ggatttcttg cagatggatt atgcgaccaa ggctagtctg
     3421 gatttggttg agaatgctcg ctcaggtaag aaacaaggca gtcttttctg gcttttggat
     3481 gaaaccaaaa cggctatggg gatgcgtctc ttgcgttctt ggattcatcg ccccttgatt
     3541 gataaggaac gaatcgtcca acgtcaagaa gtagtgcagg tctttctcga ccatttcttt
     3601 gagcgtagtg acttgacaga cagtctcaag ggtgtttatg acattgagcg cttggctagt
     3661 cgtgtttctt ttggcaaaac caatccaaag gatctcttgc agttggcgac taccttgtct
     3721 agtgtgccac ggattcgtgc gattttagaa gggatggagc aacctgctct agcctatctc
     3781 atcgcacaac tggatgcaat ccctgagttg gagagtttga ttagcgcagc gattgctcct
     3841 gaagctcctc atgtgattac agatggggga attatccgga ctggatttga tgagacttta
     3901 gacaagtatc gttgcgttct cagagaaggg actagctgga ttgctgagat tgaggctaag
     3961 gagcgagaaa actctggtat cagcacgctc aagattgact acaataaaaa ggatggctac
     4021 tattttcatg tgaccaattc gcaactggga aatgtgccag cccacttttt ccgcaaggcg
     4081 acgctgaaaa actcagaacg ctttggaacc gaagaattag cccgtatcga gggagatatg
     4141 cttgaggcgc gtgagaagtc agccaacctc gaatacgaaa tatttatgcg cattcgtgaa
     4201 gaggtcggca agtacatcca gcgtttacaa gctttagccc aaggaattgc gacggttgat
     4261 gtcttacaga gtctggcggt tgtggctgaa acccagcatt tgattcgacc tgagtttggt
     4321 gacgattcac aaattgatat ccggaaaggg cgccatgctg tcgttgaaaa ggttatgggg
     4381 gctcagacct atattccaaa tacgattcag atggcagaag ataccagtat tcaactgatt
     4441 acagggccaa acatgagtgg gaagtctacc tatatgcgtc agttagccat gacggcggtt
     4501 atggcccagc tgggttccta tgttcctgct gaaagcgccc atttaccgat ttttgatgcg
     4561 atttttaccc gtatcggagc agcagatgac ttggtttcgg gtcagtcaac ctttatggtg
     4621 gagatgatgg aggccaataa tgccatttcg catgcgacca agaactctct cattctcttt
     4681 gatgaattgg gacgtggaac tgcaacttat gacgggatgg ctcttgctca gtccatcatc
     4741 gaatatatcc atgagcacat cggagctaag accctctttg cgacccacta ccatgagttg
     4801 actagtctgg agtctagttt acaacacttg gtcaatgtcc acgtggcaac tttggagcag
     4861 gatgggcagg tcaccttcct tcacaagatt gaaccgggac cagctgataa atcctacggt
     4921 atccatgttg ccaagattgc tggcttgcca gcagaccttt tagcaagggc ggataagatt
     4981 ttgactcagc tagagaatca aggaacagag aatcctcctc ccatgagaca aactagtgct
     5041 gtcactgaac agatttcact ctttgatagg gcagaagagc atcctatcct agcagaatta
     5101 gctaaactgg atgtgtataa tatgacacct atgcaggtta tgaatgtctt agtagagtta
     5161 aaacagaaac tataaaacca agactcacta gttaatctag ctgtagcaag gagacttctt
     5221 tgacaattct ccactttttt gctagaatga catcacacaa acagaatgaa aaggagctga
     5281 cgcattgtcg ctcccttttg tctatttttt aaggagaaag tatgctgatt cagaaaataa
     5341 aaacctacaa gtggcaggcc ctggcttcgc tcctgatgac aggcttgatg gttgctagtt
     5401 cacttctgca accgcgttat ctgcaggaag tcttaggcgc cctccttact gggaaatatg
     5461 aagctattta tagtatcggg gcttggttga ttggtgtggc cgtagtcggt ctagttgctg
     5521 gtggactcaa tgttgtcctc gcagcctata ttgcccaagg agtttcatcc gaccttcggg
     5581 aggatgcctt ccgtaaaatt caaacctttt cttatgctga tattgaacaa tttaatgcgg
     5641 gaaatctagt cgttcgaatg acaaatgata tcaaccagat tcagaacgtt gtcatgatga
     5701 ccttccagat tcttttcaga cttcccctct tgttcatcgg ttcgtttatc ctagcggttc
     5761 aaaccttacc ttctctgtgg tgggtgattg ttctcatggt agtcttgatt tttggtttga
     5821 ctgctgtcat gatgggaatg atggggcctc gttttgccaa gtttcaaacc cttcttgagc
     5881 gcatcaatgc cattgccaag gaaaatttac gtggcgttcg tgtggtcaag tcctttgtcc
     5941 aagaaaaaga gcaatttgct aagtttacag aggtctcaga cgagcttctt ggtcaaaacc
     6001 tttacattgg ttatgccttt tcagtagtgg aaccctttat gatgttggtt ggttacgggg
     6061 cggtcttcct ctctatttgg ctggtcgcgg gaatggttca gtcggatccg tctgttgttg
     6121 gttccatcgc ttcttttgtt aattacctaa gccagattat ctttaccatt gttatggttg
     6181 gatttttggg aaattctgtc agccgtgcca tgatttccat gcgtcgtatt cgagaaattc
     6241 ttgacgcaga gccagctatg accttcaagg atgtcccaga tgaagagttg gttggaagcc
     6301 ttagctttga aaatgtgacc tttacctatc caatggacaa ggaaccgatg ctgaaagatg
     6361 tgagctttac tattgaacct ggtcaaatgg ttggtgtagt tggagcgact ggtgcaggaa
     6421 agtcaacctt ggctcaattg attccacgtc tctttgatcc acaggaaggg gctatcaaaa
     6481 tcggaggtaa ggatattcga gaagtgagtg aaggaaccct gcgtaaaaca gtttccatcg
     6541 ttctccaacg tgccattctt tttagtggaa cgattgcaga taacttgaga caggggaagg
     6601 gaaatgctac tctatttgaa atggagcgcg cagccaatat tgcccaggct agtgaattca
     6661 ttcatcgtat ggagaaaacc tttgaaagtc cagttgaaga acggggaacc aatttctctg
     6721 gtggacaaaa acaaaggatg tcgattgcgc gtgggattgt cagcaatcca cgtattctga
     6781 tttttgatga ttcgacctca gccttggatg ccaaatcaga gcgcttggtg caagaagctt
     6841 tgaataagga cttgaagggg acgacaacca ttattattgc tcaaaaaatt agctcggttg
     6901 tccatgcaga caagatcttg gttctaaatc aaggacgatt gattggtcaa ggcacgcatg
     6961 cagacttggt tgccaacaat gccgtttacc gtgaaatcta tgaaacacag aaa
//
LOCUS       DAVBCW010000097         6896 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVBCW010000097 DAVBCW010000000
VERSION     DAVBCW010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6896
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.097"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(251..1393)
                     /locus_tag="VQ514_001698"
     CDS             complement(251..1393)
                     /locus_tag="VQ514_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET2500436.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEAPKEVASSSEVTKTV
                     IASEEVAPSTGTSVPEEQTAETSSAVAEEAPQGTTPAEKQETQASPQAASAVEATTTS
                     SEAKEVASSNGATAAVSTYQPEETKIISTTYEAPAAPDYAGLAVAKSENAGLQPQTAA
                     FKEEIANLFGITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGI
                     SYIIWKQRFYAPFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            complement(1559..2179)
                     /locus_tag="VQ514_001699"
     CDS             complement(1559..2179)
                     /locus_tag="VQ514_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET2500437.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQNLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNTFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            complement(2183..3463)
                     /locus_tag="VQ514_001700"
     CDS             complement(2183..3463)
                     /locus_tag="VQ514_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET2500438.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTILSRLVGLVILWSQLKLPYGKLTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            complement(3524..5008)
                     /locus_tag="VQ514_001701"
     CDS             complement(3524..5008)
                     /locus_tag="VQ514_001701"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET2500439.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPVTYPKVDLDF
                     DKLKDASYQEVAKLVLSAFLDDFTAEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKGGVSKVQELQMTTQNGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGEKVNFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTAELMNALNTQGQYKLTDFDAEILDLFAAEYATEEETAAEIKRVYESDS
                     YIEDPHTAVASAVYRKYQAVTGDVTKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEIAPIRHKTTVAAADMQAAVEAYLGL"
     gene            complement(5084..6361)
                     /locus_tag="VQ514_001702"
     CDS             complement(5084..6361)
                     /locus_tag="VQ514_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HET2500440.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     IKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATSEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            complement(6468..6551)
                     /locus_tag="VQ514_001703"
     tRNA            complement(6468..6551)
                     /locus_tag="VQ514_001703"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6515..6517),aa:Leu,seq:caa)
     gene            complement(6561..6632)
                     /locus_tag="VQ514_001704"
     tRNA            complement(6561..6632)
                     /locus_tag="VQ514_001704"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6598..6600),aa:Gln,seq:ttg)
     gene            complement(6646..6718)
                     /locus_tag="VQ514_001705"
     tRNA            complement(6646..6718)
                     /locus_tag="VQ514_001705"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6683..6685),aa:His,seq:gtg)
     gene            complement(6733..6803)
                     /locus_tag="VQ514_001706"
     tRNA            complement(6733..6803)
                     /locus_tag="VQ514_001706"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6769..6771),aa:Trp,seq:cca)
     gene            complement(6808..6888)
                     /locus_tag="VQ514_001707"
     tRNA            complement(6808..6888)
                     /locus_tag="VQ514_001707"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6852..6854),aa:Tyr,seq:gta)
BASE COUNT         2088 a         1617 c         1224 g         1967 t
ORIGIN      
        1 aagaatgttg cttagattat tacaagatga taactttcca ttttccattc aaattgatac
       61 tcaatgaaaa tcaaaaagca aactaggaag ctagtcgcag gttgctcaaa gcacagcttt
      121 gaggtttcag ataaaactga cgtggtttga agagattttc gaagagtatg acaaagaaaa
      181 aacgaacgag aatcgcttcc catcacgaaa gctagatctc attcgtcaaa atgatgttac
      241 caagtcgggt ttatccattc attgaaacgt gaacgtgatc atagtgattt tctgtcacac
      301 taccacggtc tggcattggg ttccaagtgt tagctggccc atatttgcta tcgaatggag
      361 catagaaacg ttgtttccag atgatgtaac taatgccacg gctggccatg ttttgaatag
      421 catattccgc aatcttatcc cctaattctg aacgttctgg taccataaag tcgatagcca
      481 aaccttttcc gtgatctcca ctgtctcctg gacgataacc gctaaaggat gtaatgccaa
      541 acaagttagc aatttcttct ttaaaggcag ctgtttgtgg ttgaagacct gcattttcag
      601 attttgctac tgcaagtcca gcataatcgg gcgcagctgg agcctcgtaa gttgttgaaa
      661 ttattttcgt ctcttctggt tgataagtag aaactgctgc tgtagctcca tttgatgatg
      721 ctacttcttt tgcttctgaa cttgttgtag ttgcttccac tgctgatgca gcttgagggc
      781 ttgcttgtgt ttcctgcttc tcagctggag tcgttccctg aggagcttct tctgcaactg
      841 cactgcttgt ttcggccgtt tgctcctctg ggacagaagt gcccgtagat ggtgccactt
      901 cttcagaagc aatcactgtc tttgtaactt ctgaacttga tgctacttct tttggagctt
      961 ctgcaattgg ttgagaaagg tctgcaacct gaacagtttg atcatcaacg gtcacttgat
     1021 tagtggtcaa atctgctgtc gcagttgtca cttcttcact agagtctgct tgaggtgttt
     1081 ggatttcaac ttctgttact tcttctgctt cattgacagt cgttgtcaaa acagtttctg
     1141 ggaaaatcaa gtccatatta gtgattttgt tcagattcgc aagcactgtg acatctacac
     1201 ccaaggcttc tgcaatggtg ctcaaagtat caccatactg tacggtataa cttgttttgt
     1261 tgtccgtttt agtcaaatcg ttttggattt gctcaacact acgtgcagtc caaagaactt
     1321 cttctgcttg agttgccaat actggggcaa atgacaaggc tactgttgac gctaataaca
     1381 ttcttttctt cattctttca aattcctttc aaatgagtac ctgtctatca taacacaaaa
     1441 aatgtagaaa aaaagccctc tcgggcctat ttaacagaac tgtctattcc ttgtaacaaa
     1501 ctagattact tgaaatagtt tgttaggtct ctgtcacaaa actgacacaa tctttttatc
     1561 acttagtctc aaaaatacgg gaaatatctg ctaacgcttg aatcctgtga ttcccttcat
     1621 aagtagactc taaaaagttg atactttgaa tcccactatt ctgggcaaat tccacatcca
     1681 gagtccgatc ccctatataa taagtattat cagaattcaa ctgatactta tctagcagat
     1741 aggtagccgc ttctggactt ggcttccgca caaagccact ctgactggtc aaaatctctg
     1801 taaaatagga ttccaccccc aagtccttga gaatggtaaa agtgttgttc cccttatgag
     1861 tatatataaa ctgctgaatt cctgattcgt ctgcccaagc tagcacctca cgcgcacctg
     1921 gcatcaaaac tacctgagca ttcttctcag ccagactctg ggcacgcacc tgatttagca
     1981 cctcaacatc cagatttcta tcttctgcca cccgcacaag caaattttgc accgaatact
     2041 tgaagataaa ctctctcacc ttctccttat cataaggaat agaaaactga gcaaaagtct
     2101 cctcaatccc tgataaaatc gcttcgtaag agtccaataa agtcccgtct aaatcccaaa
     2161 taaaagctgt tttttgcatt tcctatcctt tcaagctcat atagcgctgg taacgatagc
     2221 gtagaaataa ccagcgaaaa ccattatcca agagagaccc tgcccaaata ccaggcaagc
     2281 cccaaccaag cacaataccc atcagatatc ctgtcccaat gcggatacac cacatgccta
     2341 tacttgtcgc ataaaaaggg aggcgtgcat ttcctaatcc ctgccagact gccgtataga
     2401 tgactgttcc tgtcgtcata ggggtaccaa gtagtgaaaa cagtgtcact agaacactag
     2461 cctccaccgc tagagaatca gtcgtataga gatgagttaa tggtacaccc aagacatata
     2521 tactaaagga caggggcaac atgaggaaca gagaaagcca aaaggtttgt ttactcaaac
     2581 tagcaactct tttccaatca tcctctccaa ctgctcgggc caacagcatg accgttgccg
     2641 tagcgacacc aaaggcaggc atatagttaa actgggtcaa gacttctccg attgcattcc
     2701 cagcaactgc ctccgtccca aaagaaacga ccaaggcaat gatcactaca tctccagccc
     2761 tcatcataag tcgctctcca gctgctggta aagccaaggt caacagttcc ttatctaaac
     2821 caaaagttag cttcccataa ggcagtttta attgtgacca caaaatcaca agaccaacca
     2881 aacgagacag aattgtcccc caagcaacac cagctatccc catatccaga acaaaaatag
     2941 ctagacttga aaaaagaata ttcaaggcat tggataaaaa actaacatag agaggcagac
     3001 gtggattatg cgttgcacga atcaaggctc ccaaactagt cattaaacct aagagaacaa
     3061 tcgatccgcc taccaaagat agatacagtc caccactctc agctacatcc ctctccgtcc
     3121 ccaaaagtcc tatcatctct ttcccagcga agatggacaa aaatcctaaa aggaaactta
     3181 atagtaaggt aatcttcaac gcctcagtca catgataggc caacttcgac tggtctttct
     3241 gccctatgct ttttgaaata acactggaaa tagcagctcc cagagcgatg aaaatcgcct
     3301 gataaatggt gataatatta ccagctactg aaacccctga aatagctatc aatcctaaat
     3361 gagcaaccaa ataactgtcc accattccca ttagcatctg caaaaagttt tcacccatag
     3421 ctggcaatgc aatattaaga atgtctttat ttttcttaaa caatctctcc tcctgatgaa
     3481 aagaaactca gttggtttcc caaccgagtt tgctccctct gtcttaaagt cctaaataag
     3541 cctcaaccgc tgcttgcatg tcagcagctg ccactgttgt cttgtgacga attggagcta
     3601 tttcaagccc atcaactgct ggtggcactg caacgcctga gatttcatgt aattgagcca
     3661 aggcttcaaa gtctgtcaaa cctgcttttc cagttacagc ttctactgca actactggga
     3721 acttgtatgg actagctgtt gaagcaatca ctgtcttagt tacatctcca gtaaccgctt
     3781 ggtattttct ataaactgct gaggcaaccg ccgtatgtgg atcctcaata taagaatctg
     3841 actcataaac acgcttgatt tctgccgctg tttcttcctc agtcgcatat tcagctgcaa
     3901 agaggtccaa aatctctgca tcaaagtctg tcaacttata ttgtccttgc gtgttcaagg
     3961 cattcataag ttcagctgtc ttttcagcat tatttcccaa aagatggaaa atcaagcgct
     4021 ccaagtttga agataccaag atatccatag atgggctggt tgttacctta aactcacgtt
     4081 ttttgtcata gacacgtgtt ttaaagaagt ctgtcaagac attattgtca tttgaagcac
     4141 agattagctt gccaactggt aggccgattt gtttggcata aaaggcagcc aagatatttc
     4201 caaagtttcc tgttggtact gtgaagttaa ccttttcacc agctacaatt tcaccagtct
     4261 taaccaattg agcgtaagca taaacataat aaacaatttg tggcaccaga cgaccaatgt
     4321 tcatagagtt agctgatgaa aattgcaact tgttggtagt caatttttca cgaagagcca
     4381 cgtcgttaaa catgtgcttc acatttgttt gcgcatcgtc aaaattacca tcaatagcaa
     4441 taacatgagt attgtcacca ttctgagtgg tcatttgcaa ctcttgtacc ttgctgacac
     4501 caccctttgg ataaaagacg ataatctcag taccaggcac atccgcaaac cccgccatag
     4561 cagctttccc cgtgtcacca gatgtcgctg tcaagataac aatcttgttc tccaaaccat
     4621 gtttcttagc agcagtcgtc ataaagtatg gcaaaataga caaggccata tccttaaagg
     4681 caatcgttga accatggaaa agttccaaat tgtattgccc atctaatttc actaatggtg
     4741 caatagctgg agtatcaaat ttgctatcgt aggcattgtt gatacagtag tccaactcct
     4801 cagctgtaaa gtcatctaaa aatgctgaca aaactagctt agcaacttcc tggtaagaag
     4861 catctttcaa tttgtcaaag tccaaatcta cctttggata agtaaccggt gtaaacaaac
     4921 cgccgtccgt cgccaaacct tgcaaaattg cttggctggc agttactgta ttgttggcat
     4981 cacgcgttga ttgataaact aatgtcatta ctctctatcc tttatctata gtctcgtcca
     5041 ttatatcatg atttttcaga aaattctccc tttataagag aaattatagg ctcaattctt
     5101 ctctcaaagg ctgtaaatag gtcatttctt ccttatttct caccactagt ccctcctcag
     5161 ctagtaggag taagtctttg gaaaattcaa taatcgtagt ttcttcctca tctgtaagat
     5221 ttttcttaga aaattgtctc cttaaagact tgtaatcata accaaatact ttaaagaaag
     5281 gtgctgtttc taagtaagct tctaacttgt ctaaattaac caataatccc aagtgaaaag
     5341 ctgcagaagc aaaagtccta tcaagtggct gtgtacacac actacgaaac tcaactgttc
     5401 ctcgagtcgt taaatcttgg tactggtaac tacgatgagt ttcaaaatcc ttctcttggg
     5461 ggtaaataat aacctcatcc ccattcagag caaatgcttg gatttcggac gtagccaaat
     5521 agtccccagc ctgaatagga taaaaataat aggtctgccc atcacgttcc gcagtaaaaa
     5581 tcgcagaatg atttagatag tcaaaaaaat cagcttcatc attaaggagt ctagcattga
     5641 ccccaacatt ctctggatag ataccatgca tagattcttc ccagaaaata tcccttgaaa
     5701 ttttcgtatc ccaatccgca cccgaaaatt cagagtttgc aaataaataa gccttagccg
     5761 cttcaatttg agtaaaagca ttaatcaccc gtaagtagtt ggtttttgaa atatccagct
     5821 gaacctggct cccacagata aaagtaccat attcagggaa atgatgtaaa tctgatttaa
     5881 taatatttct actcaaattc aaataatcca tcaacatctg atagcgtgga taagccactg
     5941 gacaattctc atttttatcc cagttgggat ggataccaca gccaacaata gcatgatttg
     6001 attcagctaa ctttctctga attacattca tataattatt gaaacgattt tcgacctctt
     6061 gaatcgtttc agccttacca aatgcaaact caatcgttgt ataggaaact tcaaataaaa
     6121 tagcatcctg acttatcgga tctactaact ggatcagatt gccaaaatca tctacctttg
     6181 ctacggtgag atccaaagta gaaactaaat aatgaaatag atcctttata acttcaacat
     6241 ctgtagcatc cccttctaaa cttgcaacag gatactccaa ctcaattccg acaaacaatt
     6301 caggattttc tttaatattt ttcaaatacc gatgttttaa taaatcgata gaacaagaca
     6361 tactacccta cactttctta atctaaataa aatttgaata actataatta taccaaaaat
     6421 aatataaaaa aggaacgaag aataacaacg ttcctaacac tatactatac cggcggccgg
     6481 ggtcgaaccg gcacgtcctt gcggacactg gattttgagt ccagcgcgtc tgccaattcc
     6541 gccacgccgg caaatagtaa ctggggtagc tggattcgaa ccaacgcatg agggagtcaa
     6601 agtcccttgc cttaccgctt ggctataccc caataatata aaataggcga gtgatgggga
     6661 tcgaacccac gcatgccaga gccacaatct ggtgtgttaa ccacttcacc acacccgcca
     6721 taattctatt aacacgggca gtaggaattg aacccacact gaaggttttg gagaccttag
     6781 ttctaccttt aaactatgcc cgtaaaatgg aaggggaggg attcgaaccc ccgaacccga
     6841 aggagcggat ttacagtccg ccgcgtttag cctcttcgct acccttccaa aatata
//
LOCUS       DAVBCW010000098         6810 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVBCW010000098 DAVBCW010000000
VERSION     DAVBCW010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6810
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.098"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(147..494)
                     /locus_tag="VQ514_001708"
     CDS             complement(147..494)
                     /locus_tag="VQ514_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET2500441.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(605..2380)
                     /gene="spxB"
                     /locus_tag="VQ514_001709"
     CDS             complement(605..2380)
                     /gene="spxB"
                     /locus_tag="VQ514_001709"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HET2500442.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAISKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIASALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            complement(2587..4830)
                     /locus_tag="VQ514_001710"
     CDS             complement(2587..4830)
                     /locus_tag="VQ514_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET2500443.1"
                     /translation="MTEIVKASLENGIQKIRIRAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYIVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLTGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDIIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQSLHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLAISQLKKRGLRTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            complement(4840..5211)
                     /locus_tag="VQ514_001711"
     CDS             complement(4840..5211)
                     /locus_tag="VQ514_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HET2500444.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEITPE
                     QVGEFGFACGMNMMHGKMIVE"
     gene            complement(5222..5617)
                     /locus_tag="VQ514_001712"
     CDS             complement(5222..5617)
                     /locus_tag="VQ514_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HET2500445.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTIQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     regulatory      5801..5899
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5983..6774
                     /gene="thiD"
                     /locus_tag="VQ514_001713"
     CDS             5983..6774
                     /gene="thiD"
                     /locus_tag="VQ514_001713"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET2500446.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEGMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
BASE COUNT         1979 a         1529 c         1285 g         2017 t
ORIGIN      
        1 tggaatagaa tgctttttga aagcagatga gtattatatg caatttcttt atataaaaag
       61 accggattgc tccgatcttt caatagttca tattctcaat ttctatttta aaaatagcta
      121 aggttaacgt caaatgacta cgcgacctat ttcatacgat aaaaatcaag cactagacca
      181 gcaggtcctt gaactaataa ggactctgtt ccccaatcgg ttacagttgg tccgtgtaaa
      241 acctttatac caagctcatt caaccgtttg tagttctggt ctacatcctc aacctcgata
      301 tgaataatga ttcctgactg aaagttttcc aaaggaacca aatgattttg tgacaacata
      361 aggcagtgac taccaatcgt aaactgagca aaaccatcat cagcataatc tgccttttta
      421 tccaaaatat gctccaagtc agcacagact tggggaacat ttgaaacgat aatatctaat
      481 tgatttaaat tcatttactc tcctccataa aaagaccgaa ttgctccgat cttttaaagt
      541 tctgctctat gaaaatcaaa gaataaagtc tacaagtttc atatttgatt ttcggcgaga
      601 ggaattattt aattgcgcgt gattgcaatc cttcttcttc caagaagaga cggaatggta
      661 cgagttcttc tgcttcgtat ttttccttga aggctttgat agcttcttct gagtgaagtt
      721 ttggatccaa ttcaagtact tctactggaa gtggacggtg ttgagtgatg cgagcatcga
      781 tgacaacagt tttaccttct ttgttcaatt taacagcttc tgcaacaact gcatcgatgt
      841 cttcgatacg gtcaactgtg aatccaacag ctccttgagc ttccgcaatt ttagcgtagt
      901 cagcgtttgt gaagtctaca ccaaacaagt gtttgtttgt atcttcgtat ttgttcttga
      961 tgaagccgta ctcagcattt gagaagacaa ggttgataac tggaaggtcg tattgaacgt
     1021 ttgtgataac gtctgggtag cacatgttga atgctccatc acccatgatg ttccatactt
     1081 ggcgatctgg attgtctttc ttagcagcga taccaccagg aagggcaata cccattgtcg
     1141 caaagagcgg agatgtacgc cacatattct taggtgtcat gtgaaggtga cgagtagatg
     1201 tttgagtagt gttacctacg tcgattgagt agatagcgtc ttgatcagca tgtttgttga
     1261 ttgcattgta aacttgatac aattgcaatt caccctcagt tttaccttcg agtttgttca
     1321 tgtaatcacg ccagttttgg ttgttcttaa cgtttgcacg ccaccatgga gttgattcaa
     1381 ctgggtttac tttgtcaagg atagctttag ctgcttgacc agcatcacca aggattgaag
     1441 cgtcaagggc atgacgttta ccaagtttgt aagggtcgat atcgacttgg atgaattttt
     1501 cagtgttctt gaatgcttcg taaacttcag caaatgggaa gtttgaacca aggaaaagaa
     1561 ctgtgtctgc ttcaaagacc acttcgttgg ctggtttcca accaacacgg taagcagaac
     1621 ctgtcaaacc ttcatagttc cattcgaaag cttcaaagtt tttaccagtt gtgatgattg
     1681 gtgctttgat tttacgtgac aattcagtaa tcacttcacc agctttaaca ccaccaaatc
     1741 cagcatagat aactgggcgt tcagcattgt tcaagatttc aacagctttg tcgatttcaa
     1801 cttcgttcaa agcagaagcg atgaatgagc gttcgtatga acctgaaccg tagtatgagt
     1861 tttcgtcgat ttcttggaaa ccgaagttta ctggaatttc aacaacagct ggaccttttt
     1921 tagaaattgc agcacggcag gcttcgtcaa ttacttttgg caattgctca gcgtaagcta
     1981 cacgtttgtt gtaaacagcg ataccgttgt acattgggtt ttggttaagc tcttggaaag
     2041 catccatgtt caattcgtta actggacgtg atccaaggat cgctaggaat ggagtgttat
     2101 ccatagctgc atcgtaaaca ccgttaatca agtgagtcgc acctggacca cctgaaccaa
     2161 ctgcaacccc gattgagccg ccgaatttag cttgcataac cgctgcaaga gcacctgtct
     2221 cttcgtggcg aacttgtaag aagcggatat ctttgtcttc agccaaagcg tccatcaatg
     2281 agctgagtgt tcctgatggg ataccgtaga ttgtatctac gccccatgtt ttcaatacgt
     2341 taagcattgc tgcagatgca gtaattttcc cttgagtcat aatgataact ctccttcaat
     2401 ttttttagat ttggagaata cgattacata gaattggaaa cgttctccaa atttttacta
     2461 ttccactgta tcatatttat gctgactttt ctaaaaatct gctcaaaact ctctattctc
     2521 tattctaata cagttttgaa agttctgtca tttctgtttt ataacaaaga aatctagtca
     2581 ttacttttag tctattttac taaaatttaa cagaagggaa ctggtcagaa cagatacaga
     2641 actaaaggcc atggctagac ctgccaattc tgggttgaga gccagtccaa cacctgaaaa
     2701 gactcctgct gcaatcggaa ttccgacaac attgtagata aaagcccaga aaagattgag
     2761 tagaattcga tgaaaggttt tcttactcat atcaaaggca cgaaccactc ctaaaagatt
     2821 attggttgtc aacaccaaat ctgctgactc gatggcgata tctgttccag ctcccatagc
     2881 aatccccaca tctgctacac taagggcagg agcgtcattg ataccgtccc caacaaaggc
     2941 tactttccct gactgttgca gtttatggat ttcatgggct ttttcttctg gcaagacgcc
     3001 tgcaatgacc tcttcaattc cgatttgatc tgcaatagca cgcgccacac cagcattgtc
     3061 tcctgtcagc atgactgttc ggagaccacg tttttttagc tgactgatgg ctagcttagc
     3121 attttcctta ggaatatctt gcaaagcaag caagcctttg atttcattgt caacagctaa
     3181 gaacacaact gtcttagctt ctttttctag ttcttctagt ttatcttgat aagtattaga
     3241 aatatccata ccatccagca ttttagcatt tccaagtaaa acttgttttc cattgattcg
     3301 ccctgaaaca cctttcccgt gcaaggactg aaaattttca acagtttgaa actcaagtcc
     3361 agcttcactc gctcgcttaa cgatagcctc agccagtggg tgttgagaag catcttccaa
     3421 ggaggctgcc aatccaaaca cttctacttc gtcgccgatg atatctgtta ccacaggttt
     3481 cccttccgtc aaagtcccgg tcttatcaaa gacaagagtt tgaactttct ggatttcctg
     3541 taagacagtt ccatttttga ggagaacccc catcttggca ctacgtcctg tccccaccat
     3601 aagggctgtc ggtgttgcaa gtcccaaggc acaaggacag gcgataatca aaaccgccac
     3661 tccgtagaga agagaggaca caaagctagc tccaagcacg accacactat ccctgagcaa
     3721 gacgaaccaa acccaaaagg tcatgattcc taaaatgaca actactggga caaaaatccc
     3781 tgaaatctta tccgtcaagt cctgaatcgg cgcacgactt gtctgagctt tcttcacaaa
     3841 atccacaatc tgagccaaaa cagtctctga gccaactttt tctgctctaa agacaagcgt
     3901 tccactatga ttgatggttg agccaatgac agtatctcca actgtcttgt ccacaggcag
     3961 actctcacct gtcaccatgg attcgtcaat actagagaca ccttctacta cgacaccatc
     4021 aacagcaatc ttttcaccgg gacgcactcg aatcaggtcg cctaccttga cttgttccaa
     4081 aggaacttgg acataactat catcactcaa gacttctgcg gttttagctt gcaagtccag
     4141 taatttctcc acagcttggg acgtattttt tctcattttt tcctcaaaaa ctgctcccaa
     4201 aagaacgaaa aagaggataa atccagcact ttcgaagtaa acagggagac cagcaaagag
     4261 agcaactagg ctatagaaat aagccactag agttcccagc gcaaccaagg tatccatgtt
     4321 ggcattgtgc tttttaaaac tggcccaagc actctggata tatggcttac ctgcaactaa
     4381 cataataggc gttgttgcta gaaaggttcc ccaatgcatg acttgatgac taatgctacc
     4441 tgtcaacatc ccaatcatga gaatcacaag aggcacagta aagatactag taatccaaaa
     4501 acgttgcagg agagatagag attttcgagt cttctcaacg actatatagc ttcccttttg
     4561 catcttcatg ccacaagaaa attcatgtcg ccctaattct tgaggtgtaa aacgaatgac
     4621 tttctcctca tctacgccga ttggttccaa gataccttct tcttcaaaca gaatttcctt
     4681 ataacagttt gaaggagtag cacgatgaaa ggtaatctca gctggaattc ccttttgaag
     4741 ctggatatgg gctggatgat agcctttttc agctcggata cggatttttt gaatgccatt
     4801 ttctaagctt gctttcacaa tttctgtcat agtctccacc tactctacaa tcatcttgcc
     4861 gtgcatcatg ttcataccac aagcaaagcc aaactctcca acctgctcag gcgtgatttc
     4921 cactacatac tcttccccca ttggcaggtt cgcatgtaca ccaaaatctg gaaaaacaat
     4981 ttgatccaga catggtgaag gatccttgcg gtcaaagaca atgcgtgctg gcactgattt
     5041 cttgaggaca atcaactcag gagtatagcc tcccatgact tccactcgaa tctcttggta
     5101 tccgtttttt tgctgggctt tttgtccaga tttttcaggc tttttgaaaa accaaaacaa
     5161 gataaacgcg ataagggcaa tacaaataat ggttacaata ctatttaaca tgacgtctcc
     5221 tttacataca attacatctt acttctgtta cagcacttga tttcttctct gaaatcacag
     5281 cttccaagtc ttccaagtca gtctgagtaa attcacattc tacaatcaag tcagccaaca
     5341 aattcctaat cctacgggaa caaaccttgt ctttgatatc ttggacaagt aaatcccgac
     5401 tttggtctag agttaaaagg gctgaataaa caaaggactt gccttctttt ttccgagtca
     5461 gacactcttt ctcaaccaaa cgagccaaaa gagtttgaat ggtcgacttg gaccagtcga
     5521 accgctctgc caaaacccta atcaaatctg tactggtctg ctccccctgc atccaaataa
     5581 tcttcatgac ctgccattct gcatctgaaa tctgcattac catacctcca aaatctacat
     5641 ttgtcaatta cactcatcag tatactctta aaatctacat ttgtcaatta tagaaataat
     5701 attttcttcg aaaaatagaa ttttaatcat ttgaaaaacg atttgcagtc aaatattact
     5761 atataaacaa taaaaatatg ctatactaaa gaaaaaagaa aacaaccact aggggtgcgt
     5821 aaagctgaga ttaacgactg ttagatccct ctgactcaat ctaggtaatg ctagctgatg
     5881 gaagtggaaa tgataatggg gactagcagt cttctattgc ctttctaaaa cagactagct
     5941 tgttcttaag aatacaaact tcagttggtt gggaggtttt agatgactta tttacccgtt
     6001 gctttgacca ttgcagggac tgaccctagt ggtggtgctg gcattatggc agatttaaag
     6061 tcattccaag cgagagatgt ctatggaatg gctgttgtaa ccagtcttgt cgctcaaaat
     6121 accagaggtg ttcagctaat cgagcacgtt tctcctcaaa tgttgaaagc ccaattggag
     6181 agtgtctttt ctgatattcc acctcaggct gtaaaaactg gaatgttggc tactactgaa
     6241 atcatggaaa tcatccaacc ctatcttaaa aaactggatt gtccctatgt ccttgatcct
     6301 gttatggttg ctacaagtgg agatgccttg attgactcaa gtgctagaga ctatctcaaa
     6361 acaaacttac tacctctagc aactattatt acgccaaatc ttcctgaagc agaagagatt
     6421 gttggttttt caatccatga ccccgaaggc atgcagcgtg ctggtcgcct gattttaaaa
     6481 gaatttggtc ctcagtctgt ggttatcaaa ggcggacatc tcaaaggtgg tgctaaagat
     6541 ttcctcttta ccaagaatga acaatttgtc tgggaaagcc cacgaattca aacctgtcac
     6601 acccatggta ctggatgtac ctttgctgca gtgattactg ctgaactagc caagggcaag
     6661 agtctttacc aggcagttga taaggccaag gcctttatca caaaagctat tcaagatgcc
     6721 cctcaactcg gtcatggttc tggtccagtc aaccatacaa cttttaaaga ttaagaaaaa
     6781 aactctctag ttcccacttt aagggaatta
//
LOCUS       DAVBCW010000099         6800 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVBCW010000099 DAVBCW010000000
VERSION     DAVBCW010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6800
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.099"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            <1..316
                     /locus_tag="VQ514_001714"
     CDS             <1..316
                     /locus_tag="VQ514_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2500447.1"
                     /translation="ELIAPMTYEETMTSDFFEVWFQKFFLPTLTTPSVIIMDNVRFHR
                     MGKLELLCEEFGHKLLPLPPYSPEYNPIEKTWVHIKKHLKKVLPSCNTFYEALLSCSC
                     FN"
     gene            377..1339
                     /locus_tag="VQ514_001715"
     CDS             377..1339
                     /locus_tag="VQ514_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET2500448.1"
                     /translation="MNMSNITIDGQEMDLSQTMPSYAQVVSLYDDTSISVSSNETDKV
                     LAGSLYTDTNEQGLTIPSSLLKNWNEQTGKNLTANDLIGKSVSASIVESAAETSKIAQ
                     FQTKIVRVINDEDDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTE
                     ELQKNKKYTVLSQQRVLDIVIIFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRS
                     KEIGVMKAVGYQNRDVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSIT
                     KVFELNLLSVLGTLVFALLLGYVSAYFPARKISKMDPVEPLRYE"
     gene            1516..2523
                     /gene="gap"
                     /locus_tag="VQ514_001716"
     CDS             1516..2523
                     /gene="gap"
                     /locus_tag="VQ514_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET2500449.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFIKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2590..3426)
                     /locus_tag="VQ514_001717"
     CDS             complement(2590..3426)
                     /locus_tag="VQ514_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET2500450.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWNEIVNPGD
                     VCQLTFDEEDYPQKTIPWGNPDLVQEVYQDQYLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETYVSRLKQFSNKTSLARCKLKTGRTHQIR
                     VHLSHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLEKLTFTTLSNTFEKELKK
                     NG"
     gene            3552..5747
                     /locus_tag="VQ514_001718"
     CDS             3552..5747
                     /locus_tag="VQ514_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HET2500451.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMASQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVAAVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYIMAGKTGTTEAVFNPEYTSDQWVIAYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            5800..5952
                     /gene="rpmG"
                     /locus_tag="VQ514_001719"
     CDS             5800..5952
                     /gene="rpmG"
                     /locus_tag="VQ514_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET2500452.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            5962..6138
                     /gene="secE"
                     /locus_tag="VQ514_001720"
     CDS             5962..6138
                     /gene="secE"
                     /locus_tag="VQ514_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HET2500453.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            6194..6730
                     /gene="nusG"
                     /locus_tag="VQ514_001721"
     CDS             6194..6730
                     /gene="nusG"
                     /locus_tag="VQ514_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HET2500454.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAEL"
BASE COUNT         2110 a         1190 c         1475 g         2025 t
ORIGIN      
        1 tgaattaatc gctccaatga cttacgaaga gacgatgacg agcgactttt ttgaagtatg
       61 gtttcagaag tttttcttac caacattaac cacaccatcg gttattatta tggataatgt
      121 aagattccat agaatgggga agctagaact cttgtgtgaa gagtttgggc ataaactttt
      181 acctcttcct ccctactcac ctgagtacaa tcctattgag aaaacatggg ttcatatcaa
      241 aaagcacctc aaaaaggtat taccaagttg caataccttt tacgaggctc ttttgtcttg
      301 ttcttgtttc aattgactat atatcacgga tacagttggg aaagaagcta ttaaacattt
      361 ggagagtcct tttgggatga acatgtcaaa tattactata gatgggcaag aaatggatct
      421 gagtcaaacg atgccttctt atgctcaagt agtgagtctt tatgatgata caagtatctc
      481 tgttagtagt aatgagacgg acaaagtgtt ggctggctcc ctctatactg atacaaatga
      541 acagggatta acgattccaa gcagtttact aaaaaattgg aatgaacaga caggaaaaaa
      601 tttgacagct aatgatctta ttggcaaatc agtctcagcc agcattgtag aaagtgctgc
      661 cgaaactagt aagattgctc aatttcaaac gaagattgta cgtgtaatca atgatgaaga
      721 tgacatggag gacagcaaca gtttcatgct gtctcatcaa atggaaacga ttttgaaaga
      781 ggctggattt acgaaagctg tatcttattt tatcttggaa ctcaaagatc catcacagac
      841 aaaagtagta acagaagaat tacagaaaaa taagaagtat actgtgcttt ctcaacagag
      901 agttcttgat attgtgatta tctttattcg tgttattcag ggattattga ttgtgctttc
      961 atcacaagct attgtggtag cagcggttat gattggtatc attatttaca tcaatatcat
     1021 gcagcgttcc aaggaaatag gtgtcatgaa ggcagttggt tatcagaatc gtgatgtcaa
     1081 aggaattttt atttacgagg ctatctggat tgtaggcatc gccttgctgc tggcattttt
     1141 ggttgcacaa ggggtgggaa gtttggcgaa tgcgattgta agtcactttt acccatccat
     1201 cactaaagtt tttgaattaa atcttttatc tgttttagga actctagttt tcgctctatt
     1261 acttggttat gtctcagcct acttcccggc gcgtaagatt agtaaaatgg atcctgtaga
     1321 accgttacgc tatgaatagt aagggattcc ttgatttaca gtcttaaaaa gaaactaaca
     1381 tagaaaaaca atggacatac ttgcaattca actcaaatag ttgtataata ggtttgttga
     1441 aaggtgattt gtagtgattc aagttactct tttcacaata aaaatttcat gattttcata
     1501 aggaggaaat cactaatggt agttaaagtt ggtattaacg gtttcggacg tatcggtcgt
     1561 cttgctttcc gtcgtatcca aaacgtagaa ggtgttgaag ttacacgcat caacgacctt
     1621 acagatccag ttatgcttgc acacttgttg aaatacgaca caactcaagg tcgtttcgac
     1681 ggtactgttg aagttaaaga aggtggattt gaagttaacg gtaaattcat caaagtttct
     1741 gctgaacgtg atccagaaca aatcgactgg gctactgacg gtgtagaaat cgttcttgaa
     1801 gctactggtt tctttgctaa gaaagaagca gctgaaaaac accttaaagg tggagctaaa
     1861 aaagttgtta tcactgctcc tggtggaaac gacgttaaaa cagttgtatt caatactaac
     1921 cacgacgttc ttgacggtac tgaaacagtt atctcaggtg cttcatgtac tacaaactgc
     1981 ttggctccaa tggctaaagc tcttcaagac aactttggtg ttgttgaagg attgatgact
     2041 actatccacg cttacactgg tgaccaaatg atccttgacg gaccacaccg tggtggtgac
     2101 cttcgccgtg ctcgcgctgg tgctgcaaac atcgttccta actcaactgg tgctgcaaaa
     2161 gctatcggtc ttgtaatccc agaattgaat ggtaaacttg atggatctgc acaacgcgtt
     2221 ccaactccaa ctggatcagt tactgaattg gtagcagttc ttgaaaagaa cgttactgtt
     2281 gatgaagtga acgcagctat gaaagcagct tcaaacgaat catacggtta cacagaagat
     2341 ccaatcgtat cttcagatat cgtaggtatg tcttacggtt cattgtttga cgcaactcaa
     2401 actaaagttc ttgacgttga cggtaaacaa ttggttaaag ttgtatcatg gtacgacaac
     2461 gaaatgtcat acactgcaca acttgttcgt actcttgaat acttcgcaaa aattgctaaa
     2521 taattcttga gttgatagaa agcaaggctt tgtggtcttg ctttttatat ggaaaaatgg
     2581 atgacacgat catccattct tttttaattc tttttcaaat gtatttgaaa gggtagtgaa
     2641 agttagcttc tctaaagtaa gtgggtgggt aaaggaaagt cggaaggcat gaagcataag
     2701 ccgacttgtc tttgatttac tattatagag agggtcgccc aggataggaa gattatgatg
     2761 cgaaaggtgc acacgaatct gatgggttcg ccctgtcttt agcttgcaac gagccaagga
     2821 agtcttgttt gagaattgct ttaatctgct tacatacgtt tcagcatatt gcccattttt
     2881 tgcatcaact attctttttc tacgatcatg gcgatcacgt ccaattttgt ctctgaaaac
     2941 aagttctttt ctgttgatat ttccatcaac tagagcccaa tattctctag aaatctcttt
     3001 tttctccaat aagcgattga gaatgggcag gataaaagga tttttggcaa agagaactaa
     3061 gccactggtt tccatgtcca gacgatgaac gacatagcag gtttggccaa cataggtact
     3121 gacatggtta agaagggcaa tttcgtttgg ttgattacca tgcgttttca tcccctctgg
     3181 tttgtttaca ataatcaagt attgatcttg ataaacttcc tgcactaagt ctgggttgcc
     3241 ccaagggatc gtcttttggg gataatcttc ctcgtcaaaa gtcaactggc aaacatctcc
     3301 aggatttacg atttcgttcc agtggacttc ttcttgattt atcaaaatat gtttcttgat
     3361 tctcaaaaaa tgacggattt ttctagggat gaggagttgt tcctcaagta attgctttac
     3421 cgtcatttga ggtagagagg cgggtaatgt aaatgtgaat tgcatacaga tattgtaaca
     3481 aaaaaagccc tatttggata ggaaatagct aaattcttgt cttcctatga tgaagatgat
     3541 aaaataaacg catgaaatta gataaattat ttgagaaatt tctttctctt tttaaaaaag
     3601 aaacaagtga actagaggac tctgattcta ctatcttacg tcgctctcgt agtgatcgaa
     3661 aaaaattagc ccaagtaggt ccgattcgaa aattctggcg tcgttatcat ctaacaaaga
     3721 ttatccttat actaggtttg agtgcaggct tgctagttgg aatctatttg tttgctgtag
     3781 ccaagtcgac caatgtcaat gatttgcaaa atgccttgaa aactcggact cttatttttg
     3841 accgtgaaga aaaagaggct ggtgccttgt ctggtcaaaa gggaacctat gttgagctga
     3901 ctgatatcag taaaaacttg cagaatgctg ttattgcgac agaagaccgt tctttctata
     3961 aaaatgacgg gattaactat ggccgtttct tcttggctat tgtcactgct ggacgttcag
     4021 gtggtggctc taccattacc caacagctgg ctaaaaacgc ctatttatca caggatcaaa
     4081 ctgttgagag aaaagcgaaa gaatttttcc ttgccttaga attaagcaaa aaatatagta
     4141 aggagcaaat tctaaccatg taccttaaca acgcttattt tggaaatggt gtgtggggtg
     4201 tagaagatgc gagtaagaaa tactttggag tttctgcatc agaagtgagt ctggatcaag
     4261 ctgcgactct ggcagggatg ctcaaggggc cggaactgta taatcccttg aattccgtag
     4321 aagattctac taatcggcgc gatactgtct tgcagaatat ggttgcagca ggatatattg
     4381 ataaaaacca agaaaccgaa gctgctgaag ttgatatggc ttcgcaattg cacgataagt
     4441 atgaaggaaa aatctcagat taccgttacc cctcttattt tgatgcggtg gttaatgaag
     4501 ctgtttccaa gtataatcta acagaggaag agattgtcaa taatggctac cgcatttaca
     4561 cagagctgga ccaaaactac caagcaaata tgcagattgt ttatgaaaac acatcgctat
     4621 ttccgagggc agaggatgga acgtttgctc aatcaggaag tgtagctctc gaaccgaaaa
     4681 cagggggagt tcgtggagtt gtcggtcaag ttgctgacaa tgataaaact ggattccgga
     4741 atttcaacta tgcaacccaa tcaaagcgta gtcctggttc tacaattaag cctttagttg
     4801 tttatacgcc agcagttgaa gcaggctggg ctttgaataa gcagttggat aaccatacca
     4861 tgcagtatga cagctataag gttgataact atgcagggat caaaacgagt cgagaagttc
     4921 ctatgtatca agccttggca gaatcgctta atctacctgc tgttgccgct gttaatgatt
     4981 tgggtgtcga caaggctttt gaggcaggcg aaaaattcgg actcaacatg gaaaaggtcg
     5041 accgtgttct tggtgtcgcc ttgggaagcg gtgttgaaac caaccctctt caaatggctc
     5101 aagcatacgc tgcctttgca aatgaaggtt taatgcctga agctcatttt attagtagaa
     5161 ttgaaaatgc tagtggacaa gttattgcga gtcataaaaa ttcacaaaaa cgggtgattg
     5221 ataagtctgt agctgacaag atgaccagta tgatgttggg gactttcacc aacggtaccg
     5281 gtattagttc atcgcctgca gactatatta tggcagggaa aactggaaca actgaagcag
     5341 ttttcaatcc ggagtacaca agtgaccagt gggtaattgc ttatactccg gatgtagtga
     5401 ttagccactg gcttggcttt cctaccactg atgaaaatca ctatctagct ggctctactt
     5461 caaacggtgc agctcatgtc tttagaaaca ttgccaatac cattttacct tatacgccag
     5521 gaagtacctt tacggttgaa aatgcttata agcaaaatgg aattgcacca gccaatacaa
     5581 aaagacaagt acaaaccaat gataatagcc agacagatga taatttgtct gatattcgag
     5641 ggcgtgcgca aagtctagta gatgaggcta gccgggctat ctcagatgcg aagattaagg
     5701 aaaaggctca aacaatatgg gattcgatag tcaatctatt tcgctaagat gcttgtcaaa
     5761 gcctagcttt cttgttataa tggataggat ggaggcgtta tggcactaaa aaaagcaagc
     5821 ctagcttgtg cggtttgtgg ttcgagaaac tattcaatca agatcagcgg aaaccccaag
     5881 cctacacgac tagaagtaaa taaattttgt aagcattgtg gcaagtacac tacacacaga
     5941 gaaacgagat aggagagagc gatgcgtttt attggagata tttttagact tcttaaagac
     6001 acaacgtggc caactcgcaa ggaaagctgg agagattttc gttctatcat ggaatacaca
     6061 gctttctttg tagtaattat ttacattttt gaccagttga ttgtttcagg tttgattcga
     6121 tttattaaca ttttttagaa gattagtgga gttaattaca ctagaaatct tctatattat
     6181 gaaaggaaat atcatggata gttttgataa agggtggttt gttttacaaa cttattctgg
     6241 ttatgaaaat aaggtgaaag aaaatctatt acaacgtgca caaacctaca atatgttgga
     6301 taatattcta cgcgttgaaa ttccaacaca aacagtgcaa gttgaaaaaa atggaaagag
     6361 aaaagaagta gaagaaaatc gctttccagg ttatgttctt gtagaaatgg tcatgacaga
     6421 tgaagcttgg tttgttgttc gaaacacacc aaatgttaca ggatttgtcg gatcacacgg
     6481 gaacagatca aaaccaactc cattattgga acaagaaatt cgtgacattt tggtatctat
     6541 gggacaaact gttcaagaat ttgatttcga tgttgagatt ggtcaaactg tacgtattat
     6601 tgatggtgct tttgcagact acactggtaa gattacagaa attgataata acaaagtgaa
     6661 aatgattatc tctatgtttg gtaatgacac agttgcagaa gtaaacctaa accaaatcgc
     6721 agaattataa ccctgagaga ggctggagcc tctctttttt gtgcagttta ggagctaaag
     6781 ggaacagaat ggagaaaatg
//
LOCUS       DAVBCW010000100         6795 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVBCW010000100 DAVBCW010000000
VERSION     DAVBCW010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6795
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.100"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            60..761
                     /locus_tag="VQ514_001722"
     CDS             60..761
                     /locus_tag="VQ514_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2500455.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIQMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            763..3463
                     /locus_tag="VQ514_001723"
                     /pseudo
     CDS             763..3463
                     /locus_tag="VQ514_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
     gene            complement(3559..4050)
                     /gene="tpx"
                     /locus_tag="VQ514_001724"
     CDS             complement(3559..4050)
                     /gene="tpx"
                     /locus_tag="VQ514_001724"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HET2500456.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(4173..5102)
                     /gene="psaA"
                     /locus_tag="VQ514_001725"
     CDS             complement(4173..5102)
                     /gene="psaA"
                     /locus_tag="VQ514_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HET2500457.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            complement(5134..5982)
                     /locus_tag="VQ514_001726"
     CDS             complement(5134..5982)
                     /locus_tag="VQ514_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET2500458.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            complement(5979..6701)
                     /locus_tag="VQ514_001727"
     CDS             complement(5979..6701)
                     /locus_tag="VQ514_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HET2500459.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
BASE COUNT         1957 a         1329 c         1530 g         1979 t
ORIGIN      
        1 gacgtggttt gaagagagat tttcgaggag tataatttgt ttgatagagg gtgggtctga
       61 tggcttatat tgagatgaaa cactgttaca agcgttatca ggttggggac acggagattg
      121 tggccaatcg tgatgtgaat tttgagattg aaaaggggga gctggttatt atccttggtg
      181 cttcaggtgc aggcaagtca acagttctta accttcttgg gggaatggat accaatgatg
      241 aaggggaaat ctggattgat ggtgttaata ttgcggatta tagttcccac cagcgtacca
      301 attaccgtag aaatgatgtg gggtttgttt ttcagtttta taatctagtt tctaatctga
      361 cagctaagga aaatgtggaa ctggcttctg aaattgtgac agatgccttg aatcctgatc
      421 aggtcttgac agatgtaggt ctggctcatc gtctcaataa ctttccagcc cagctttctg
      481 gaggggagca acagcgagtc tccattgcac gcgcggtagc caaaaatcct aaaattctcc
      541 tttgtgatga accgactgga gccttggatt atcagacggg caagcaggtt ttgaaaattc
      601 tccaagacat gtctcgtcaa aagggagcga cggtgatcat cgtgactcat aatggagctt
      661 tggcgcccat tgctgatcgc gtgattcaaa tgcacgatgc cagtgtcaag gatgtggtgc
      721 tcaaccagca tcctcaggat attgacagtt tggagtacta gcatgatcaa gcgaaaaact
      781 tattggaagg acttaattca gtccttcaca ggctccaagg ggcgtttttt atccatcttg
      841 atcctgatga tgttgggatc tctagcctta gtaggcctca aagtaaccag tcccaacatg
      901 gaggcgacag ctaatgctta tttaacaact gctcaaacct tggatttggc agtcatgtct
      961 aactatggct tggatcaagc agaccaagaa gaactaaaac agacggaggg cgcagaggtc
     1021 gagtttggct atttgacaga tgtgactatg gataatgggc aggatgccat tcggctgtac
     1081 tccaaaccag agcgaatttc aacctttcag ctaagaaagg gacgacttcc tcagtcagac
     1141 aaggaaatcg ctttggccac tcatttgcaa ggccaataca gcgtgggaca ggagattagt
     1201 tttaaagaaa aagaagaggg tcattcctct ttaaaagacc atacttatac cattactggt
     1261 tttgtggatt cggctgaaat cctctcccag cgagatatgg gctacgcagg aagtggaagt
     1321 gggactctga cagcctatgg ggtgatttta cctagtcagt ttgatcagaa agtctacaat
     1381 atagctcgtt tgaaatatca agatttagca ggtttaaatg ccttttcatc agcttatgaa
     1441 gaaaaatcca agcaacatca ggaagacctt gaacaaactt tatcagataa tggcaaggta
     1501 cgtctgcaac ttttgaaaaa agaaggacaa gagtctctag acaaggggca agagaccctt
     1561 gacaaggctc agactaattt gcaggaaggc aagcgtcgtt tagcagctgc tcaagctcgt
     1621 atacaggctc aagaaagtca actagccttg tttcctcaag ttcagagaga gcaggctagt
     1681 gctcaactta cccaagccaa gcaggaattg ggcaaggaag aggataaact aaagcaagct
     1741 gaacaaaatc tagcccaaga aaaggaaaag ttagaaaaac atcagcaagt cttggacgat
     1801 ttggcggagc caaggtatca ggtctataat cgtcagacca tgccaggtgg tcagggctat
     1861 cttatgtata gcaatgcttc atccagtatt cgagcagtgg gcaatatctt tcctgtggta
     1921 ctttatgccg tagcatccat ggtgaccttt acgaccatga ctcgctttgt agacgaagag
     1981 cgaactcatg cagggatttt taaggccttg ggttaccgta gtaaggatat tatcaccaag
     2041 tttctccttt atggactagt agctgggact gtcggaacgg ctctaggtag tatacttggt
     2101 cattatttgc tagccagtgt aatttcaagt gtcattacaa aaggcatggt ggtgggagaa
     2161 acccagattc agttctattg gacctatagc ttactagctc ttgtcttgag ctggttggcg
     2221 agtgtgttac cagcctatct ggtggcttgg agggaacttc atgccgaagc agcccagctt
     2281 ctacttccta aacctcctgt caaaggagct aaaatcttat tggagcgtat cggttttatc
     2341 tggcgtcgtc tcagttttac tcataaggta acagcccgca acatctttcg ttataagcag
     2401 agaatgttga tgacaatctt tggtgtggca ggttctgtag ctctgctctt tgcaggtttg
     2461 ggaatccaat cttctgtagc aggagttccg tctaaacagt ttcaacaaat ccaacagtat
     2521 cagatgcttg tctctgaaaa tcctagtgcg accaatcagg acaaggtaga gctagcagaa
     2581 gtgttgaaag ggcaggagat actagcctac cagaaaatct attctaaaac gctagacaag
     2641 gatttcaaag gcaaggctgg tcttcaaaac attactctta tgatgataga gaaggaagat
     2701 ttgactccct ttatccatct tcaacatcat cagcaggagc tgacattaaa agatggcatc
     2761 gttattacag ctaaactcgc ccagctggca ggtgtcaagg ttgggcagac tttagaaatt
     2821 gaaggtaagg aactaaaggt cgctgctatt actgagaact acgttggtca ctttatttat
     2881 atgagtcagg ctagctatga gcaactttac ggacagctac cccaagccaa cacttatctg
     2941 gtctcattaa gggataccag tgcaactagt atcgaaagtc aggcgggctt gcttatgaat
     3001 caatctgcgg tgtccagcgt tgtccaaaat gcttcagcca ttcgactcta tcgctagctc
     3061 actcaatcag accatgacca tcttggtcat cgtatcagtt ctattagcta ttgtcatcct
     3121 ttacaatctg accaatatca acgtagctga gagaatccgt gaactctcca ctatcaaggt
     3181 tctcggtttt cataataatg aagtcaccct ctacatttac cgtgagacga ttgtgctgtc
     3241 ccttgtggga atcgtacttg gtctggtagc tggtttctat ttacaccaat ttttgattca
     3301 aatgatttcg cctgcgacta ttctctttta tccgcaggta ggctgggaag tctatgtaat
     3361 cccagtggca acagtaagca tcattttgac cttgcttggt ttcttcgtca attattatct
     3421 gagaaaggtt gatatgttag aagccctgaa atctgtagag taaggtagtt atttttagct
     3481 gattgaactt ctatttacta atattcaaaa atcctctgtc acaaagagca gggagctctt
     3541 tgtgacagag gattttttct atagggcttt agcagctgca attgcggctt cgaagtttgg
     3601 ctcagaattg atattatcca cgtattcaac gtagcgaatc gtattgtcag tatcgaggac
     3661 aaagactgcg cgtgctaata ggtgccattc gttgatcaag agggcataat cgcgcccgaa
     3721 agaatggtca aagtagtctg aaagcataat ggcattgtca aggccttcag caccgcacca
     3781 acgtttttga gcaaaaggta ggtccattga aacagtcaat acgaccgtgt tgtccagtcc
     3841 agccaattct tcattaaaac gacgtgtttg agttgagcag atgcctgtat cgatagaagg
     3901 aacgacactc aagacttttt tcttgccatc aaaatcagcc agagattttt tagaaagatc
     3961 tgttgtagta agagaaaaat caagcgcctt gtcgccgact tgtagttgtt tacctgtaaa
     4021 gctcacagga tttccgagaa aagttaccat aggatactcc aatctttttt cttccattgt
     4081 atctgaaaca gtcagaattt tccaatgatt tgaccggaaa tgtgggcata gaaaaaacgc
     4141 cagctcacat gagaatgacg tttttcagag gcttattttg ccaatccttc agcaatcttg
     4201 tcaaggttgt atttcatcat gctgtagtag ctgtcgcctt ctttaccttg ttctgcgata
     4261 gagtcagtaa agatttgtgc gtagattggg atgtttgtgt cttgagaaac agttttcatt
     4321 ggacggtcat ccacacttga ttctacaaag agtgatggaa cttttgtttg gcgaagtttt
     4381 tcaaccaagg tcttgatttg ttcaggagtt ccttcttctt cagtattgat ttcccagatg
     4441 taagcacttg ggacaccata ggctttagag aagtatttga atgctccttc gctggttaca
     4501 atgagtttct tttcagcagg gatcttatta aatttatcct tactttcttt atcaagtttg
     4561 tctaacttat cagtatattc tttgagattt ttttcataga attctttatt gttagggtct
     4621 ttggcgctca attgtttggc gatattttta gcaaaaataa taccgttttc aaggttaagc
     4681 caagcgtgtg ggtcttcttt tcctttttca ttttgacctt caaggtagat aacatcaacg
     4741 ccgtcgctga ctgcgaagta gtctttgttt tcagttttct tggcattttc taccaatttt
     4801 gtaaaccaag cattgccacc tgtttcaagg ttgataccgt tatagaaaat caaatcagcc
     4861 tcagaagttt tcttaacgtc ttcaggaagt ggttcgtatt cgtgtgggtc ttgcccaatc
     4921 ggaacgatac tatgaaggtc aattttgtca ccagcaatat ttttagtaat atcagcgatg
     4981 attgagtttg tagcaacaac ttttagtttt tgaccagaag ttgtatcttt ttttccgcta
     5041 gcacatgcta caagaatgat tgcagaaaga aagagaacga gtaatgtacc taattttttc
     5101 attagatcct ccaaattatt agggctttgc cccttatttt aacaaatgtt tatttttcag
     5161 tttcaaatat cgttgtttgg gagcgataaa gaagctaatg agaaagaaac tagcagctgt
     5221 aagcacgata ctagaacctg ccgcaacatt aaaactatag ccaataaaga gtcccaaaac
     5281 tgaagcagta gctccgaagg ttgaggaaag gaaaatcatg cttttcagac tattagcata
     5341 cagataagca gttgcagctg gggtaatcag catggctaca atcaggatag ttccgacact
     5401 ttgcatggct gtcacagaca cgagagtcag gagtaccatg agaaggtagt gatagaaatt
     5461 gacaggcatt cccatggctt tagccaagag ttcatcaaag gaagttatca agagttgctt
     5521 gaagaaaatc cagattaaca agaggatggc tgcccccaca cccatagtaa taaacatatc
     5581 cgtatcttgg acggccagga tattaccaaa aaggatatgg aaaaggtcag ttgaactttt
     5641 agcgacacca atcaagatga taccgagggc taagaaagaa gaaaaggtaa tgccgatggc
     5701 ggtatcgctt ttgataatcg agtttccttt gatgtaggta atgatgatgg cagctagcaa
     5761 tccaaagaca atggctccga taaagaagtc aaggcccaag atgaaggaga gggctacacc
     5821 tggtaagaca gcatgtgaaa tggcatctcc catgagtgac atcccgcgta gaataatgaa
     5881 acatcccaca gctccagcta caatcccgac gacaatagct gttatcaagg cattttgtag
     5941 gaaatggaat ttttgcaatc catcgataaa ttctgcaatc ataggtcacc tccattgaaa
     6001 aagagttgat taccgtaagc ttcttttaga ttggtttcgg taaaagtttc ttttgttgga
     6061 ccaaaggcaa tcacttctcg attgacaagt aagacttgat cgaagtagtg gggaatcttg
     6121 ctgaggtcgt ggtgaacgat gagaaccgtc ttcccagctt ttttcaaatc tctcagcgta
     6181 ttcatgatga tttcctcact gacagagtca atcccagcaa agggttcatc caagaggata
     6241 tagtcggctt cctgcaccaa acatctggca atcaaaaccc gctggaattg acctccagac
     6301 agttgactaa tttgacgttc agcgtagtca gctaggccga cgatttcaag ggcctcttgc
     6361 actttcttcc aatgtttagc ctttaaactt cgaaagagag gaatagaggg aaatagtcct
     6421 aacgagacgc attccttgac cttgatggga aagttgtagt cgatattgat tttttgttcg
     6481 acataggcaa ttcggtgtaa ggatttttta acttccttgt catcgagaaa tgcctgacct
     6541 tgatgtggga taattcccag catacctttt aatagtgttg atttcccagc gccgtttgga
     6601 ccaatgatgc cggtaattgt tggtccatgg agcactagtg aaatatcctt aagtgccaac
     6661 gtttctttgt aggagacact gaggttttcg atacgtatca taaacttata ttcctcctgt
     6721 ctcttaatat acattaaaaa aaaaattaag tcaagttaat ttttgaaaaa attaaaataa
     6781 taactgaaaa ataga
//
LOCUS       DAVBCW010000101         6750 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVBCW010000101 DAVBCW010000000
VERSION     DAVBCW010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6750
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.101"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(117..1541)
                     /locus_tag="VQ514_001728"
     CDS             complement(117..1541)
                     /locus_tag="VQ514_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HET2500460.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAIERAAYLHKTAAILERDKEKIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMLKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2085..4364)
                     /gene="pulA"
                     /locus_tag="VQ514_001729"
     CDS             complement(2085..4364)
                     /gene="pulA"
                     /locus_tag="VQ514_001729"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HET2500461.1"
                     /translation="MYSYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKTYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGCLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4476..6434)
                     /gene="ligA"
                     /locus_tag="VQ514_001730"
     CDS             complement(4476..6434)
                     /gene="ligA"
                     /locus_tag="VQ514_001730"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HET2500462.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KASQLLLQYFHSIENLSQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSVSKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6527..>6750)
                     /locus_tag="VQ514_001731"
     CDS             complement(6527..>6750)
                     /locus_tag="VQ514_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2500463.1"
                     /translation="FFTQVQTKVESEFLGRVLSTIFTLAILFMPIAKGFMTVLPSVHL
                     SSFLIIGSGVIILSCISFIYVRTHFEKLI"
BASE COUNT         1984 a         1493 c         1226 g         2047 t
ORIGIN      
        1 ctgtactatt ctagtttcaa tatactataa taccaaaaag ttcgtatttt ttctataatt
       61 attattattt tatagcaaaa aataaaacca ggaaaacaat ttcctgattt tacacgttat
      121 ttcacatcaa aaacaatgga tttgacattt gtcatcgctt caatgctata tttaattcct
      181 tgcactccag ctccagaacc tttgacacca aggaatggga aattatctgg accacgctgg
      241 gttttattat taatgtggac tgtacctact tcaagttttt cagcaatttc aaaggctttt
      301 ttgaaatcat ttgtaaagac tgatgattga aggccgaatt cagattcgtt ggcaaaggca
      361 atagcttcct ctacactagc cacacgaatg attggtaaaa caggaccaaa tggctcttcc
      421 catgccactt tcatatcttt tgtaacttgg tcaaaaagca ctggccagag gagattgccc
      481 tcacgtttga ttggtgtaag agcctgagcc tctttttctt gtgcatcctc aatcaagccc
      541 caaatgaagt cggctgaagc attgtcaata acaggtgtaa tatcagcatt gtcaaatgga
      601 tcaccaactg ttaatttaga aacttcttcc tgaagcaaag tagctaattt atctgctaca
      661 ctttcgagaa caatgacacg tttaatggcc gtgcaacgtt gtcctgagta gctaaaggct
      721 cccgcaacaa tttgcttggc agcatgttcc aaatctgcat cttctagtac aagagctgca
      781 tctttcccac caagttccaa catgatagga cgcataccag ctaaacgacc aatacgttct
      841 ccaataggag ttgaacctgt aaagttgatg aagttgactt ctttgtgctc aatgatataa
      901 tccccaattt ctgaaccacg acctgtaatg gtgttgaaaa cacctgccgg aatccctgct
      961 tcttcaaatg ctttagccaa caagagtcca gaaatggaac cttgtgttgg tggcttcaac
     1021 atgaccacat tccctgcaat caaggcaggt gcaattttag aagcagataa attaactgga
     1081 taattaaagg gagcaatcgc tagcacgata ccaactggtt cacgacggac aacagccagt
     1141 ttgtttttac ttgctgcctc aaaaccacca ccttccattg cttgtccagt gatacggaga
     1201 ccttcctcag cagcataacg aatcaagtct gctgtacgca ctacttctcc aattgctgct
     1261 ttaatccctt ttgctacttc tttggcaagg atagtaccaa ttttttcctt atcgcgttct
     1321 aaaatagctg ctgttttatg caaataagcc gcacgttcaa ttgctgataa agctcgccat
     1381 gctggcaggg ctgcacgcgc agcttgcata gcctcatcag cttcagtctg agtcatggct
     1441 ggaactgtac ccaattcttc ttgattgatt ggtgaataaa tcgtaatttc ttgttcagat
     1501 gatttccatt ttccatttac taaattctga tatcttgtca aattcttgtc ctcctgaaaa
     1561 tgattaagat atattttacc aaattttcag aaaataacaa ccctttttgc aaaatttttg
     1621 agattatttt cacaagcttg atttttcaaa gtatactcaa taaaaataaa aaaatccact
     1681 acgtcaaggc gaggctaatg tggtttgaag aaattttcga agagcgtgaa tgagtatcat
     1741 ctatagtaaa ataaaaaaac tgaacaattt ggttggggac agccaaacca atttctcaca
     1801 atgtttcaga aacaagggtg tgctattcca atttcagcct actataactg tcatagattg
     1861 ctgaaacaaa gtctaggtaa aagtcttcat aataaaaaga cctcctatca aatgttcaaa
     1921 aactttgata ggaggtcttg tttcgtgaaa atatttatca aattttctat acaagtgagc
     1981 tgttagccag gttctttcta ttctttcaat ttcaatgaat ggatttttta ccaatactca
     2041 taactgggaa tttttctgtc taaaaatagc gagatagatg gtatttataa aacactcaag
     2101 acagctagac taatatcatt taaaacatta tcttcttttg agcgactgtt ggttaccaac
     2161 atagctaaat ttcctgcatt ttcaaattga tagggttctg atttagcatt cacaactacc
     2221 aagaggtgtt ctttgccgtg aacttcatag ataaggcagc cgctatgttc aatcgcagaa
     2281 tgcacaaaga catgatggta aatttcatca tagctagagt aagaaaaggc accagttttt
     2341 gtcttcaatc ggatgacttg acggataaac tcaatactgt cttgacgctc attaatcaag
     2401 ttccagttca cttggttcac actgtcagga gcattatagc tattcatcgc acgctctcta
     2461 tcatcatggg tcaactcacc attttcacca gtcgcaacca gtttggtacg accaaattct
     2521 tgaccgattt ccataaaggc catcccctgc atgagcagat tcatggctgt ggcagtttcg
     2581 accttgcgca tgatttgctc tgaactttgg tctggatgaa gggttgccaa taaatcgtga
     2641 agattgtaat tgtcatgggc ttctacatag ttaagcacct gatttggatg tgtatagctt
     2701 cctaattcac gacttcctag gattgcttta gctagaattg gctctgtcgc agcaccactg
     2761 acaaaacctg acttgatagc accataaact tctccccctt tgacagcatc gcgctgattg
     2821 tcattaaaga aaccaatatt tggcatctgg taggcattgt ccttcttggc cttatcataa
     2881 ggggcaagac ctgttcccat atcccatcct tctccataga ggataatgtt ggagtcaatt
     2941 tcatccaagc tttgacgaat catctgcatg gtcttgacat catgaatccc catcaagtca
     3001 aaacggaagc cgtcaatatt atattcctgc acccagtata gaagagaatc aatcatatac
     3061 ttgcgaaaca tttcgtgttc actggctgtt tcatttccaa cacccgttcc attctggaag
     3121 gtaccatctg gattcatacg ataatagtaa tcagggactg ttgtttggaa tggtgcatca
     3181 acaactgaga aggtatggtt atagactaca tccataatga ctccaatacc cgcatcgtga
     3241 taagcttgaa ccatcacctt caaatcacga atgacctgag ctggatcatc tggattagtt
     3301 gaaaaactag tttctggcgc gttatagttt tgtggatcat aaccccagtt gtaggttaca
     3361 tttccatcct catcgtattc tttatgacgg tctgcaattg gttgcaattg aacataattg
     3421 tagcccagct tcttgatgta atcaaaagca gttgactggc cgtattggtt aactgttcca
     3481 gcctgagcag cacccaagaa agttcctcga agatgttcat ctacacccga tgtaggtgat
     3541 ttagtcaaat cacgaatatg catttcacag ataactgcct tacatggatt ttccaagcgc
     3601 caagtagcct ccgaaccgtg cttaacctcg aagttttcaa cttgcttttc tacatggctc
     3661 agaatagctg aacgtttgcc atcagggctg gtcgcgattg tataaggatc acgtgtcagt
     3721 gtttggtgat gagggaattg gacttgatac tgataagtct tacctaccaa atcttcttca
     3781 acatccaaac tccagacacc gattgtattg tccttatgat tataagagta gctattgcct
     3841 cttttcatct caaaagtctt ccaaacgggt gcatcattag cagctgattc ataaacgaca
     3901 acttgcactt ctgtcgctgt aggtgaccag agggaaaaat gagcctgatt gtcctctaca
     3961 cggcaaccca attctccttg gtaaccccaa tgatgatcaa aactagcact gttaatggcc
     4021 ttatcaaagg caaaaggatt ttgattttta tagaaaggac tggcaatagc aggattttca
     4081 gagtaataaa tcctatcatc gccttccaaa atccagacct ctgttaatag gggatagtga
     4141 ttaaaacgga tagaatattc tttactagtt tgacctgtat gaaccacaaa attcaagctt
     4201 tctataacat gtgaacttgg gtgttcaaag ctaaataaag ctccaaaata atcttctttg
     4261 taggttagca aatcaattcg ttgatcctga ctttttacaa aggagcaagt gtcatattct
     4321 ccattcttac gatggtaatg aatgcgcata gggtagctat acatttttta tttttccttt
     4381 ttactttgtt tctatttcac taataaattt ttgtcaatct cgtctcaatt aacagacata
     4441 gtcatattct ctaaactctg tttttaaacg atccattaca aactttctag ccatgcctca
     4501 tctctgacct ggataccaag ttcttgtgct ttttgcagtt tacttccagc gtctgcacct
     4561 accacgacga ggtcggtctt tttagaaaca ctacctgtca ctttggcacc cagactttcg
     4621 agtttacttt tagcttctga gcgcttgagt cgttccaatt ttcctgtcaa taccacggtc
     4681 aaacctgaca aggccgcatc cgctactacc gtctgtcctt tatagtccag attgacccca
     4741 gtttctttca attctctgag cagaatttca gagccttctg tcgcaaaata agtctgaaga
     4801 cttttggcaa tcacgccacc tagactttca atactagcca cttcctctga atctgcctga
     4861 gacagatttt caattgaatg gaaatattga agtaaaagct gactagcctt gcttccgaca
     4921 tgacgaattc ccaaaccaaa taaaagcttc tcggcagaat tttcctttga tgcttggata
     4981 gcctgataca gtttagcagc ggacttttcc ttaaccccct ctaaaaggag gaaatcctct
     5041 tcttgcaaac gataaatatc cgccacatcc ttgactaaat tagcagcaaa aagcttctca
     5101 acaatagatg gaccaaggcc tgtaatattc atagcatcac gagaagcaaa gtgaatcaag
     5161 ccttccatga tttgagcagg gcaacgcgga ttgatacaac gtagggccac ttcatcttca
     5221 aagtgcaaca agtcagagtt acaacttgga cagtttgtag ggatatctag tttttcttca
     5281 gaaacccgtt tggactctac cacacgtaaa acggcaggga tgatgtcacc agccttatat
     5341 acaatgaccg tatcgtcttt tcggatatct ttttcagcaa tataatctac attgtgcagg
     5401 gtcgcacggc taacagtcgt accggcaagt tgtactggtg ttagattagc agttggagtt
     5461 acaacaccgg tacggccaac tgtccagtca actgataaga gttgagcttc tttttcttca
     5521 gcagggaact tgtaggctac tgcccacttt ggagccttaa cggtaaaacc aagttcttct
     5581 tgacttgcta ggtcgttgac cttgattacc actccatcaa tatcgtaagg cagattttcc
     5641 cgttcctgtc ctacttcttg gataaaattc cagatttcat ctatgttttc agccaagatt
     5701 cgcttaggat tgaccacaaa acctagttgt tctaggtact tcaaaccctt ttcttggcta
     5761 tcacgagttg aagggctggc ttcttgatag agaaacgttg caagattacg cttggcaact
     5821 actgctgtat ccaactgacg cagagttcct gctgccgcat tacgaggatt agcaaattca
     5881 ggctctccat tttcttggcg cacttggtta acttggtcaa aggaagcgcg tggcatgtaa
     5941 cattccccac gaactgtgat atctagttct tctggcaaag tcaaagggat gtccttaaca
     6001 cgcttgaggt tttctgtgat attttcacca attgaaccat ctccacgtgt taccccagca
     6061 accaaaatcc ccttttcata agtcagcgag atagataagc catcgatttt cagctcacaa
     6121 atataggtcg gatgagccac ttccttacga acacgcgcat caaaagcatc tagctcctca
     6181 cgtgaaaaag catcctgcaa actataaaga ggatactgat gactgtattt ttcaaaacca
     6241 tctaaaacct tgccaccaac acgatgagtc ggactgtctg ctagcacttg ctctggataa
     6301 gcagtttcta actcgaccaa ctcacggtaa aggcggtcat actcactgtc tgaaaccgag
     6361 ggattatcgc tggtatagta ctcagtcgca tagcgattga gcaaagcgac taactcattc
     6421 attcttttat tcataagacc attttaccat aaaacaagcc ctcctcacaa acgagaaggg
     6481 cggaaaaaac acttagtttg aaattatttt tgaaactcaa gcaaccttat atcaattttt
     6541 caaaatgagt tcgaacataa ataaacgata tacaagacaa gatgataaca ccacttccaa
     6601 ttatcaggaa agaagagaga tgtacacttg gcaagactgt cataaatcct tttgcaatag
     6661 gcataaatag aatagctaag gtaaaaattg tactcagtac tcttccaaga aattcgctct
     6721 caaccttggt ttgtacttga gtaaaaaagt
//
LOCUS       DAVBCW010000102         6711 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVBCW010000102 DAVBCW010000000
VERSION     DAVBCW010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6711
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.102"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            123..845
                     /locus_tag="VQ514_001732"
     CDS             123..845
                     /locus_tag="VQ514_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HET2500464.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVMDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNDGQLAHVLLSPKRHVDKTYLAQVKGIMTQEDVETFAEGIPLKDFTC
                     QPAILELVSIDTEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
     gene            1062..2396
                     /gene="pepC"
                     /locus_tag="VQ514_001733"
     CDS             1062..2396
                     /gene="pepC"
                     /locus_tag="VQ514_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HET2500465.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQVTVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAK"
     gene            complement(2452..3363)
                     /locus_tag="VQ514_001734"
     CDS             complement(2452..3363)
                     /locus_tag="VQ514_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET2500466.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(3387..4190)
                     /locus_tag="VQ514_001735"
     CDS             complement(3387..4190)
                     /locus_tag="VQ514_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HET2500467.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            complement(4218..5207)
                     /gene="manX"
                     /locus_tag="VQ514_001736"
     CDS             complement(4218..5207)
                     /gene="manX"
                     /locus_tag="VQ514_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HET2500468.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            complement(5440..6459)
                     /gene="adhP"
                     /locus_tag="VQ514_001737"
     CDS             complement(5440..6459)
                     /gene="adhP"
                     /locus_tag="VQ514_001737"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HET2500469.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKNLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
BASE COUNT         2012 a         1387 c         1473 g         1839 t
ORIGIN      
        1 tagaactgac gaagtcagta acatatatac ggcaaggcga cgttgacgcg gtttgaagag
       61 tatgaaacga aaaggaggtt ggtcattgta ccagcctcct tttgatatga tataatagtt
      121 ctatgagatt agataaattt ttagttgcct gtgcggtggg aagtcggact gaggtcaaaa
      181 acttgctcaa ggctgggcgc gtgactgtaa atggtaaaaa agaaaaatca gctaaattgc
      241 agattgatga aaaaatagat gagattcgct ttgatgggca agtgttggag tatgaagagt
      301 ttgtctacta catgatgaac aagcccaaag gagttatctc agcgactgag gatcccaagc
      361 acagaaccgt tatggacttg ttggatgact tggcgcggag taaggaagtt ttcccagtag
      421 gacgcttgga tattgacacg catggtcttt tgctcttgac caatgatggt cagctggctc
      481 atgttcttct ttcgcccaag cgtcatgtgg acaagactta tctggcacaa gtcaagggaa
      541 tcatgaccca agaagatgtg gagacatttg ctgagggtat tcctctcaaa gactttacct
      601 gtcaacccgc tatactggag cttgtatcca tagatacaga aaagaatcaa agccaaatcc
      661 gtgtgaccat tgcagaaggg aagtttcatc agatcaagcg tatggtgggc tactgtggca
      721 aggaagtggt ggacttgcaa cgattgacta tgggaacttt agtattagat gagaacctgg
      781 aacgagggga atggcgtcgc ttgaccaagg aagaattaga aattctccgc gctaatatta
      841 tttaatttag tttaatactc aatgaaaatc aaagagcaaa ctaggaaact agccgcaggc
      901 tgtacttgag tacggcaagg cgacgttgac gtggtttgaa gagattttcg aagagtataa
      961 attcgaaaaa ggtgagggac aatgtcctcg ccttttatgt tttttagttg cttcctttgt
     1021 gaaaagagtt ataatagact gtagaataaa aggaggaatc tatgaacgcg attcaagaat
     1081 catttactga taaactattt gccaactatg aagcaaatgt aaaataccaa gcgattgaaa
     1141 atgctgccag ccacaacgga atttttgcag ctctggaacg tcgccaaagc catgtagaca
     1201 acacacctgt tttctcattg gatttaacca aggacaaggt cactaaccag aaagcgtctg
     1261 gtcgttgctg gatgtttgcg gctctcaaca ccttccgcca caaactcatc tcgcaataca
     1321 aattggagaa ctttgagttg tcacaagccc acactttctt ctgggataag tatgagaaat
     1381 caaactggtt cttggagcaa gtcattgcga cttcagacca agaattgact agccgcaagg
     1441 ttagcttctt actccaaaca cctcaacaag atggtggtca atgggatatg gtcgtttccc
     1501 tctttgaaaa atacggtgtc gtgcctaagt cagtttatcc tgagtctgtt tcatctagca
     1561 gcagtcgtga gctaaatgcg atccttaata aattgcttcg tcaagatgct caaatcttgc
     1621 gtgatttgct tgtttctggt gcagatcaag tgactgttca agctaagaaa gaagacctct
     1681 tgcaagaaat ctttaacttt cttgctatgt cattaggact tccaccacgc aagtttgact
     1741 ttgcttatcg cgataaagat aacaactaca aaagtgaaaa aggaatcaca ccacaagagt
     1801 tctacaagaa atatgtcaat cttcctttag aagactacgt ttctgttatc aatgctccaa
     1861 ctgctgataa accttacgga aaatcttaca cagttgagat gttggggaat gtggttggta
     1921 gccgtgcagt tcgttacatc aacgttccaa tggagcgctt gaaagaattg gcgattgccc
     1981 aaatgcaagc aggtgagact gtttggtttg gttctgatgt cggccagctc agtaaccgta
     2041 aggctggaat ccttgcgaca gatgtttatg actttgaatc aagcatggac attaaactta
     2101 ctcaagacaa ggctggacgt ttggactata gcgaaagctt gatgacccac gccatggtct
     2161 tgacaggtgt tgacttggac gaaaatggta aatcaaccaa gtggaaggtt gaaaactcat
     2221 ggggagacaa ggtcggtaca gatggttact ttgttgcctc agacgcttgg atggacgaat
     2281 acacatacca aatcgttgtt cgtaaggaat tgctgacagc agaagaacaa gctgcctatg
     2341 gagcagaacc aattgtactt gcaccatggg atccaatggg agccttggct aaataaaagc
     2401 atagaaaaaa ggaatcagat tttagaacct ggttcctttt tagttgcttg attacatgat
     2461 gtgaagaaca tgtgccacaa tacccactgc gaagagggca aggataatag tgattggaga
     2521 tacttttttc ttaagtaagt acatgcaaag taaagtaagg agtagtcctg ataatccagg
     2581 aatcaacata tccaagtttt gttggaaagt agtaactttt tcaggagttt gagacaatcc
     2641 ttgtcctact tgtgcgaatg cttcttggat acctttagac ccttctggca atttatccca
     2701 atggatataa gccttttcat ctagttgaac tttagaaaca tcgaaagcaa atttaatatt
     2761 tacccagcgt tgaacaagga cagcaagaat gaacatccca aggatagaag ctcctttagt
     2821 gatatcttga aggataccac cagacatatc tttagtgatt tctgatccag ccttgtatcc
     2881 aatctcttga acataccaca agaatgacat acgaatcaag ttccatgcaa caaagaagag
     2941 gagtggcccc aagatattgc cagtaagggc aagtgaagca ccgagagatc caaggattgg
     3001 gcgtactgta aaccagaata ctgggtcacc gataccagca agaggtccca tcataccgat
     3061 tttaacccct tggatagcag cgtcatcgat ttccacaccg ttagcacgtt cttcttcaag
     3121 cgcaagagta acccccatga ctggagcagc tacgtatgga tgagtgttga agaactcaag
     3181 gtgacgctca agagcagcga tttgatcttc ttttttagta tagagttttt tgatagctgg
     3241 aatgagtgta taagcccagc ccaagttttg catccgttca aagttccaag acccttgtaa
     3301 gaaggttgaa cgccaccaaa cttttttacg atctgattta gttaattgaa gtttttcagt
     3361 catgatgttt tcagtccttt cttatcttag tagtcttcta ggatatcgcc gattgggtcg
     3421 ttagaagttg cggctcctcc gccaccattt ccaccagttt tagaaaggtg aaggtagata
     3481 agagcgatag caacaccgat agcaccgaat ccgattagag taatatctga cacagcagcg
     3541 agaacgaaac caagagcgaa gaatggccat acttcacgag ttgccatcat gttgataacc
     3601 atggcgtaac caacggcaac gaccatacca ccaccgatag ccataccatc tttgagccag
     3661 tctggcatgg cactaaggat actttgtaca gtttcagttg gtaccataag gagaagagct
     3721 gcaggaagcg cgatacgaag tccttggaag agtagcgcaa tgaaatgcgc acgctccaca
     3781 gcgccgaagt cacctttttt agcggcagca tctgcagtat gaaccaaacc aactgaaatt
     3841 gtacgaacaa tcattgtcaa gaaaagtcca gctacggcaa gagggatagc aaccgcttgg
     3901 gcaacaccga taccagtctt ggtaaagtca ccaccaagaa ccatgataat ggcagcagcg
     3961 acagaagcaa gtgcagcatc aggagcgata gcagcaccga tatttgacca accaagggca
     4021 atcatttgaa gcgatccacc gaggataatc cctgcttcca agtgacctgt tacaagccca
     4081 ataagggtac aggctacaag tggttggtga aattggaact ggtcgaggat gccttcaaga
     4141 cctgcaaaga aggctacaac gactactaaa accatagaaa taatagacat gtttaaaatc
     4201 ctttcataaa taatggctta tttgacattg gctttgttaa tcaagtcaaa caaatctttt
     4261 ttagaatcat ttggtacttt acggacatca aattcaacac ccaagtcacg cattttttca
     4321 aatgtagcaa catcttcttt gtccatagac aaaacggtat tgaccaatgt tttacctgtt
     4381 gagtgagcca tagaaccaac gttaagagtc ttgattggca cgccgccttc gatggcacga
     4441 agggcatctt gaggtgtttc aaacaagata agggcatgtg tttctccaaa acgtgggtct
     4501 tttgaaatct caatcagttt ttgaattgga accacgttag ccttgacatt acctggagct
     4561 gcttgtttaa tcaattcttt acgaaggtcg tctttagcca cgttatctga agcaacgatg
     4621 atacgatttg cttttgaatc tggagtccaa gcagttgcaa cctgaccgtg aagtagacgt
     4681 gtgtcaagac gggcaagatt gattttcaat ttaccgtctc cgataacagt tccttctggg
     4741 atagcagttt gggcaactgg agcagctgca gcgcttgcaa cttcttcaac tggatttagc
     4801 tcttctggaa gagctttgat gccatctttg gcttctttaa tgatattagc agcgactttt
     4861 tctacacctg cagcagcgtc catgaggcgc tctgtgtagg cttgaatcaa catcggtaag
     4921 ttaagtcctg tgatgatggc aaacttacgc tcaggatttt ctcccatcac gcgactagct
     4981 tggttaaatg gagaaccact ccaaaggtca gccaaaacta gaacctcatc ttctgcgtca
     5041 aatgcagcaa cagcgttatt aaacttagcg tatagatcat caggaccttc atttggcata
     5101 aaggtcacaa cttgaacctt ttcttgttca ccaaagatca tagatcctga ctgatgaata
     5161 cccgcagcaa attcgccgtg gctcgcaata atgattccga tactcattat tgtcattcct
     5221 ccttttttgt tttagttttc atttaagaaa actttaagac tgtttaagta taaaccgttt
     5281 tcataaaaaa agcaactatt tatcataaaa aattaaaaaa tttttatttt tgtatgttga
     5341 tatgttgagt ttttgaggag taatcttgaa ttcttattta aaatttaata taaaaaagtt
     5401 ggaagaagct tccaactttt aaaataggtt tctattagat tagtgggtga agtcgagtac
     5461 catacgtcct tggatttggc ctttttccat ttcgtcgaaa atggcaacag catcttctac
     5521 tggacgtttt tgaacaactg ggactaccag accttctgca ccaaattgga aggcttcttc
     5581 taagtcttta cgagttccga caagagaacc gatgacttgg attccatcga ggactgtttt
     5641 aacgatgctg agttccatca tttcagaagg aagaccaaca gcgacgacgc gaccaccagc
     5701 acgaatggag tcaacagcct ggttgaaggc aactttagac acagcagtta cgacagctga
     5761 atgagctcct ccatcagttt tttctttaat gagtccagct acatcttcaa cttcgaggcc
     5821 gttaatcaca atgtcagcgc ctacttcttt tgcaagggca agtttgtcat tgttgatatc
     5881 gactgcgata acatgagcat tgaatacttt tttagcgtat tgaacagcga ggttaccaag
     5941 tccaccagca ccgtaaagaa caacccattg gcctggttca acttttgctt ctttgatagc
     6001 tttataggtt gttactccag cacatgtgat agaagaagct tgggctggat caagtccgtc
     6061 aggaactttg acagcatagt cagcagttac gatacattgt tcagccatac caccgtctac
     6121 tgagtagcca gcatttttca ctgtacggca aagggtttcg cgaccagttg tacagtattc
     6181 gcaagtgcca catccttcaa agaaccaagc aacgctgacg cggtcaccga ctttaaggtt
     6241 tttcacatct ggagcaatct ctttaacgat accgatacct tcgtgcccaa gaacacgtcc
     6301 tgggacttga ccaaagtcac catgagcaac gtggaggtcg gtgtggcaaa cgccacagta
     6361 ttcaacttct acaagtgctt ccccagtttc aagtggacgg agtacttttt cttcaatagc
     6421 aacaccagtg ctttctggat ttacaacaac agctttcata gctatcctcc ttgatattga
     6481 ccgagaacag ggacaacggg actggattga ttttttgtca acagagtcga gtgtgacgag
     6541 ctctcttgta tttatatgtg aagtatatca caaaagaaag ccttttccaa ggttttggag
     6601 acaaaaagta gaacaatcga ttaactggtt gatgagactg tgaaaagagc acaaagacca
     6661 gcttgcaagc tggtcatact cttcgaaaat ctcttcaaac cacgtcagct t
//
LOCUS       DAVBCW010000103         6594 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVBCW010000103 DAVBCW010000000
VERSION     DAVBCW010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6594
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.103"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            196..486
                     /locus_tag="VQ514_001738"
     CDS             196..486
                     /locus_tag="VQ514_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500470.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEYPELYAVAQTSGEIPEKNAKNCVRLSQLSLNKR"
     gene            510..1226
                     /locus_tag="VQ514_001739"
     CDS             510..1226
                     /locus_tag="VQ514_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET2500471.1"
                     /translation="MKKYFIGGLGSNAYHSKDFLQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEESICLIGHSIGGDLARYFASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIISDLDVLTSKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNEATWREEALKELPDYFDTIFLENFGHEL
                     YTQAPKEIASLMNEWLAYFL"
     gene            complement(1355..2170)
                     /locus_tag="VQ514_001740"
     CDS             complement(1355..2170)
                     /locus_tag="VQ514_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HET2500472.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWITAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(2167..3072)
                     /locus_tag="VQ514_001741"
     CDS             complement(2167..3072)
                     /locus_tag="VQ514_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HET2500473.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGMLFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMILAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLVYSLLIGVIL"
     gene            3470..5599
                     /locus_tag="VQ514_001742"
     CDS             3470..5599
                     /locus_tag="VQ514_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HET2500474.1"
                     /translation="MDKKYEKISQDLGVMLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRAQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYTAVKELFKRL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVSVGD
                     LVTVWVKKIDTEREKVNLSLLAPNETD"
     gene            5586..6035
                     /locus_tag="VQ514_001743"
     CDS             5586..6035
                     /locus_tag="VQ514_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HET2500475.1"
                     /translation="MKLTDYVKQVSLEDFGRAFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQKLGMEVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPLKLYRCQSCQQRYQRKRRIDTKRYRCGLCRGKLLLINQPED"
     gene            6094..6366
                     /locus_tag="VQ514_001744"
     CDS             6094..6366
                     /locus_tag="VQ514_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET2500476.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
BASE COUNT         2064 a         1203 c         1428 g         1899 t
ORIGIN      
        1 acaagacgct taaatcgtgc atcagttagt tgtttacttg cttcataatt catagaacta
       61 ctataccata ttttgtttcg caggaagtct aatgctaaaa tcttatcagt gagaagattt
      121 cttaaagttt attctttgta gtttcatctg tattagtaca taatttaact tagagaaaat
      181 aggaggacta atcgaatgga attaaaagat tttacagaaa aagaacagga aatgattaag
      241 aaaaggctta caatgtctaa tattagtgac aaagaaacta ctgagaagat tctggcgcta
      301 gtcccacaag acttgattaa gcgaatcccg ttttttgtca gaaaacatgc tacaacacgt
      361 acgattaaac gtatttcaat tgaataccct gaactctacg ctgtagctca aacaagtggt
      421 gagattccag aaaaaaacgc gaagaattgc gtcagattat cacaactatc tttgaacaaa
      481 agatgaataa gcatagtatt aagtagagaa tgaaaaaata ttttattggc ggtttgggaa
      541 gcaatgccta tcatagcaag gattttcttc aagaactaga ttcgcaggtc tattttctaa
      601 atccatatga aaagcatctt cgagatgaaa cagaattgaa atcatggttt aaaaatgaga
      661 ttgtagagga agaatctatc tgtctgatag gtcattctat tggaggcgat ttagctcgtt
      721 atttcgcatc ggaatttgaa gaagtaaaga aactgattct tttggatggt ggctatctag
      781 atttagataa gattttacct ttggatacag agttagagga aactaaaaat tatatcaaat
      841 ctcaaattat ttcggactta gatgttctta cttctaaaga aaaatctgaa gcaaagcatt
      901 ggtcagaaaa tatggagaaa gctgtaagac agtcctatca ctggaatgtt gagtataata
      961 gatatgagtt ggctataaat tatgaaaata tagaagcgat actccgccta cggagaaaaa
     1021 tacaagcttt taagagagaa gtgggagata ccttgtttat tagtcctcgc tatcctaatg
     1081 aagctacatg gagagaggaa gccctaaaag aattgccaga ctattttgat actatttttt
     1141 tagaaaactt tggccatgag ctttatactc aagcacctaa agaaatcgct agtctaatga
     1201 atgagtggct cgcttatttt ctatgagcta cttgtattgc tggaaatggt ataagatgaa
     1261 aatctacaag tcactaattt tcatataaca aaaaacgaac aggctctttt tctgttattc
     1321 agaagagaac ttgttcgttt ttatcttgat tttcttaaaa agctctatat acatagggat
     1381 tttctggttt atcgactttg gtaaagctgt cgatttccaa ggttggaagg ttttgtttga
     1441 gttctttatg gtagtgattg accagttttc ctttggctgt tatccaagtg ttgttttcat
     1501 actgccgggt attgcccttg gtcagcaatc catagacacc agaatctgcg atacagtgga
     1561 taatgccgaa tcggaacaga aattgactat tggcatgact ggggtcgtta tagacaaagc
     1621 ctgtaaactg gattgtcttg ccctcaaact catctggata gtcgtagata gcctccatga
     1681 cttccatata gttttcatta gtgatctgaa tactatcttg ggataagtat ttatccgccg
     1741 ccgttcgcat ttccttttca taggctgatt ttgaaaaata agaactggta tctggtttca
     1801 aatattggct tgtcgtccct tcgcttgtct gaatggctag atctgttcct tccgataggg
     1861 ggaaatgata acctttagca gaaacggtct gagaatccaa gctaacagtt gggaaagtta
     1921 agccgatgac aagtggaata gccagaagag aaatactcgt catcttggcc aatcggctgt
     1981 tcagatgact gtgggttttg acttgcttca tccagatata caattgaaca attgccaaga
     2041 taaaggaaag caccatggag atataggcca gataggaata gtgcatgttg atgtactggt
     2101 ttagtttgcc cgacagatgg aggtaaatag tcagttcaaa atagccagct aaaactaaaa
     2161 atcgaatcat aggatgactc caatcaagag agaatagact aagacgacaa gagttacaat
     2221 cgttatgaag tgactgataa atcgtgcttt caagtaattt ttcatcatga gaatattttt
     2281 gatatccagc attggaccaa tgacgagaaa ggccagaact ggtgccaaac cgaaactcga
     2341 gagaagagaa gcacctataa aggcatccgc ctcactacag agcgaaagaa gaaaggccaa
     2401 aatcatcaag agcaggatgg caaaaagagg agtcgcactg atagaggtca gaatccgagt
     2461 cggaacgtag acctgtatta tagaagcaaa gaggcagcca aataccaaat aacgccccgt
     2521 atcaaaaaat tcatcaatgg cctgcacaaa gacttgaaaa actttttttg cagaactcaa
     2581 gtaagaaaaa tcatgctcat gacaagccag acgattttct ttctgaatcg gttcttgcca
     2641 gaaaaatcct agaaaaattc ctagtattac agccacaaga atggaaccca gagctcgtaa
     2701 tagggcgaca tggaaggagt tgccaaaggc agaataggtc gcaaaaagaa caatgggatt
     2761 gataacaggt gctgtcacaa gaaaaggaac ggctgtgtaa ctgggaacct ttttttccag
     2821 aaaacgattg atgatgggga cgattccaca ttcacaagaa gggaaaagca taccgacaaa
     2881 ggtcccaaaa aagattctcc cccaacgatt tcgagggaga aaatgataaa ccttgtcagg
     2941 tgtgatataa acttcaatca gccctgagac aatgcttcct attagaacga agggaagggc
     3001 ttcaatgata atggagagaa aaatggctcc agtttgaagt acactagatg ggagttgttg
     3061 aaaagcagtc atctagtttt tcttttctgc tttggctttt ttctctttgt catcagggaa
     3121 agtaacttgt ttcaaatcag ggagtttagc aaactcttca aacatcttgt ccaagtcgtc
     3181 tcgttttgta aattttacag ccataggtgt acctcgattc taaagtctac ctctattata
     3241 ctattatttt gcggaaaagg gagtttttta atttgttttc gatagttgat acacttcgaa
     3301 aatctcttca aaccacgtca gcttcgcctt accgtatata tgtgacttat ccacaacctc
     3361 aaaacagtat tttgagcagc ctgcggctag cttcctagtt tgctttttga ttttcattga
     3421 gtatgaaaag gattggagaa agccttccag cttgtggtat aatagattta tggataaaaa
     3481 atatgaaaaa atctctcagg atttgggagt gatgttaaag caaattgata ccgttctaag
     3541 tttgacagct gaaggggcga ctattccctt tatcgcgcgt tatcgcaagg acatgactgg
     3601 tagtctggat gaggtggcga ttaaggctat tattgatttg gataaaagtc tgacaaatct
     3661 caatgaccgt aaggaagctg tcttagctaa gattcaagaa caaggtaagt tgaccaagga
     3721 attggaagaa gctatcttag ttgccgaaaa attagcagac gttgaagaac tctatcttcc
     3781 ttataaggaa aagcgtcgta ccaaggcaac cattgcccgt gaagctggac tctttcctct
     3841 tgctcgtttg attttgcaga atatagttga cttagagaaa gaagctgaaa agttcgtctg
     3901 tgaaggattt gcgactggca aggaagcctt gaccggtgca gttgatattt tggtcgaagc
     3961 cttatcggaa gatgtgacct tgcgttctat gacttatcag gaagtgctga gacactctaa
     4021 acttacttct caagccaagg atgaaagtct tgatgaaaag caggtttttc agatttatta
     4081 tgatttttca gagacagttg gaactatgca aggctatcgt accttggctc tcaatcgtgg
     4141 ggagaaactt ggtgtcttga agatcggttt tgaacatgcg acggaccgta ttcttgcctt
     4201 ctttgctact cgtttcaagg tgaaaaatgc ttatattgat gaagttgttc agcaatccgt
     4261 taagaaaaag gtcttgcctg ctatcgagcg tcgtattcgg acagaattaa ctgagaaagc
     4321 tgaagaagga gctatccaac ttttttctga caatctgcgc aatctcctct tggttgctcc
     4381 actgaaaggg cgcgtggttc ttggatttga cccagccttt cgtacaggtg ccaagttagc
     4441 tgtcgtggat gcaacaggaa aaatgctgac aactcaggtt atttatcctg ttaaaccagc
     4501 atcagctcgt caaatcgaag aagccaagaa agatttagca gatttaattg gtcaatacgg
     4561 tgtagagatt attgccattg gaaatggaac ggccagtcgt gaaagtgaag cttttgtagc
     4621 ggaagttctg aaagatttcc ctgaagtcag ctatgttatc gttaatgaaa gtggtgcttc
     4681 tgtctattct gccagcgaac ttgctcgtca ggagtttcca gacttgaccg ttgaaaaacg
     4741 ctctgccatt tctatcgccc gtcgtttgca agatcctctt gcggaattgg tcaaaatcga
     4801 tcctaagtca attggtgtcg gtcaatacca acacgatgtc agtcagaaga aactatctga
     4861 gagtctggac tttgttgtcg atacagtggt taaccaagtt ggtgtcaatg tcaatacagc
     4921 tagcccagct cttctttcac acgtagctgg actcaacaaa actatctctg aaaatattgt
     4981 caaataccgc gaggaagaag gaaaaatcac ttcacgcgcc caaatcaaga aagttcctcg
     5041 tctgggagcc aaggcctttg agcaggctgc tggtttcctt cgtatccctg aaagtagcaa
     5101 tatccttgat aatacaggag ttcacccaga gaattacact gcagttaagg aactcttcaa
     5161 acgtttagat attaaagacc taaacgaaga agcccaaagc aaactcaagt ccctttcagt
     5221 caaggaaatg gctcaagagc tagacctcgg tccagaaacc cttaaagata tcattgcaga
     5281 tcttctcaaa ccaggtcgag atttccgtga ttcctttgac gcacctgtgc ttcgccaaga
     5341 tgtcctagat atcaaagacc tagtggtagg tcagaagcta gaaggtgttg tgcgtaacgt
     5401 tgttgacttc ggtgccttcg ttgatatcgg gattcacgag gacggcttga ttcatatttc
     5461 tcatatgagt cgcaagttta tcaaacaccc tagtcaagtg gtgtcagttg gagatttggt
     5521 gaccgtttgg gtcaagaaaa ttgatactga acgtgaaaaa gttaatctgt cgctcctcgc
     5581 tccaaatgaa actgactgat tatgtcaagc aggtttcact agaagacttc gggagagctt
     5641 ttatccatca tgtccagtgg aataggcgtc tacgttcgac aggtgggcga tttttcccca
     5701 aagatgggca tctggatttt aatcccaagg tttatcagaa actgggtatg gaggttttta
     5761 ggaaaattgt ccgacatgaa ctctgtcatt atcaccttta tttccaaggg aagggctatc
     5821 aacacaagga tcgggatttt aaggaactgt tgaaagcagt ggatggatta aggtttgtac
     5881 catccttgcc caatagtaac tctaaaccac tcaagcttta tcgttgtcaa tcctgccagc
     5941 aaaggtatca gcgcaagcgt aggattgata ccaaacgcta tcgctgtgga ctttgtcgag
     6001 gtaaattgct tctgataaat cagcctgagg actgatgaaa gccgagcctc cccgtgatat
     6061 actatgtcta acctagcaga aagaggaaat atcatgaaca gtaaatttta taaaatgaga
     6121 cgcaatcgca tgatttcagg agttctggca ggtctgtcag acaagtggaa ttttgatgtt
     6181 accctagttc gtttcttgtt tgccattttt acagttgcaa actttggact tggcgtgatt
     6241 atctatatca tcctagcttc tatcatgcct accaaggaag aaatcgaagc agaaatgtac
     6301 ggaacaggcc cacgtaaacg caaagaagcc caagccattg acgacaatga aggctggttt
     6361 tggtgaggtt ctacagaaga tttggaataa gcaaaaagct ttaaatcaat agtctaaact
     6421 gcttgattta aagctttttt acttttatac tcaataaaaa tcaaagtgca aactaggaag
     6481 ccagccacag gctgtaattt agtcggcaat gtgaagttga catagtttgc actttgattt
     6541 ttgaatagta ttaataatat ttcgccagct tcatccacaa actatagtgg attg
//
LOCUS       DAVBCW010000104         6534 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVBCW010000104 DAVBCW010000000
VERSION     DAVBCW010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6534
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.104"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(15..245)
                     /locus_tag="VQ514_001745"
     CDS             complement(15..245)
                     /locus_tag="VQ514_001745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500477.1"
                     /translation="MSDIHVNIPGECLYDKIFVLSFIIYNISTNLNIVNGIFYIQAKK
                     DSITFEWKAKEQTRKLAIDSSKPCFEVVDIIK"
     gene            complement(865..2091)
                     /locus_tag="VQ514_001746"
     CDS             complement(865..2091)
                     /locus_tag="VQ514_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HET2500478.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVFEVGPGDEVIVPAITYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(2134..2826)
                     /locus_tag="VQ514_001747"
     CDS             complement(2134..2826)
                     /locus_tag="VQ514_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET2500479.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGNLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSTEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFKVLK"
     gene            complement(3012..4763)
                     /locus_tag="VQ514_001748"
     CDS             complement(3012..4763)
                     /locus_tag="VQ514_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2500480.1"
                     /translation="MQNKQEQWAVLKRLMSYLKPYRLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLVYANRSVALDALFLRPAMSLLKLLGYAVLMAYFGYRGLSIG
                     ITAGTMYAFIQYINRLFDPLIEVTQNFSTLQTSMVSAGRVFALVDERTYEPLQENGQA
                     KVQEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLALGG
                     TYHKMYSLQAGAMAYTL"
     gene            complement(4753..6498)
                     /locus_tag="VQ514_001749"
     CDS             complement(4753..6498)
                     /locus_tag="VQ514_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2500481.1"
                     /translation="MSIIQKLWWFFKLEKHRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSPAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGYDIRDYRLTELRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSEVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILNPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADFILVLQNGQIIERGRHEDLLAMDGWYAQT
                     YQSQQLEMKGEEDAE"
BASE COUNT         1972 a         1414 c         1375 g         1773 t
ORIGIN      
        1 gtcctattct tatttcattt tattatatct acaacctcaa agcagggttt tgagctatct
       61 atggccaact tcctagtttg ctctttagcc ttccattcaa aagtgatgct atctttctta
      121 gcttgaatat aaaatatccc atttactata tttaaatttg ttgatatgtt gtaaattata
      181 aatgaaagaa caaatatctt gtcatacaag cattcgccag ggatatttac atgaatatcg
      241 ctcatatggc ggattgaaac taaaacacta caatatattt cttaaataga ccttctataa
      301 aagctagatt taacctcgat aaaaatagat gcagtgcttc taaaatactg gaatagggca
      361 tataggtaat cgtagacaag tgttttatag attgtctcgc gggagatcta atgatgtata
      421 gcaagcatca ctaaacatat agaatatttg tgttattata atgtcaatat gctataaatt
      481 ctatcatgca aaagaatgac tagaacatga aagacaggca caaaactttg aacaatattc
      541 tgaaaacaaa aaaagacgcg agtttccaca atctcactca tggaaactca cgtctttcaa
      601 aaaatagggt gaattaaaaa ctagaacaag acactagatt ttctaaatga tgtctagtaa
      661 ggattctaaa tgctacacca aattgctcac atcttatttc aaaccacggg actcccacaa
      721 gaaaggaaaa tcctataaaa attctctctc tgaggagaga gaggcctcaa ctacaggtgt
      781 aaactattat caattgaaaa caacagtttt tacactcaat tagacttttt agggacagga
      841 tcactttcgc ttgactgtag tttgtcattt ttttgataaa gttagcactt tttcagaaac
      901 tgttttgaaa gtctcaatga tatagtctac ttcttcatcg cttaatttag tatgaagagg
      961 gagggtaatt tcattctcaa agaaggcata ggccttagga tagttcgtca tatcaaatcc
     1021 aagattctta taggctgtca agagaggaag cggtttgtag tgaacattac ttgcaattcc
     1081 tgctttagcc aattcttgga tgatgaggtt gcgttcttct aggcttgctc cttctacacg
     1141 ggtgatgtag aggtggcgtg aagattcgac agtttcagtc ttgtgtgcca aaggatggat
     1201 gcgagaacct gcaaaaccac tatcatagcg gtccacaatg tccttacggc gttgcaacaa
     1261 acttggatag cggtccaatt gtaccaaacc aagtgaagcc atgatatcgg tcatgttgca
     1321 cttataggct ggtgtaacga tatcgtattc ccatgacccc agttgcatct tggcaagagc
     1381 atccttagtt tgcccgtgaa gggaaaggat ttggaattcc ttgtacatct cttcgtcatc
     1441 aatcactgga ttggctttcc aagtcgcact tccaccttct gccgttgtaa agttcttaac
     1501 ggcatggaat gagaaggaag taaagtcagc gatagaacca gaaggttgtc ctttataagt
     1561 agatcccaaa gcgtgggcac tatcagagac aatgacaata cggttaaagg ccttttgcca
     1621 cttgcttgaa gcggtaaaga agtcacgttt tttctccacg acttggaaca aacggtcata
     1681 atcgcaaaca atccctgcga gctctactgg gataatcacc ttagttttct cagtgatagc
     1741 ttgctcaagc aggtcatagt ccatctcaaa cgtatctgct tggatatcca ccatgacagg
     1801 ggttgctccc acgtgcgtaa tgacactaca tgaagccgta taggttatgg ctggaacgat
     1861 gacttcatca ccaggtccca cttcaaaaac gcgtaaaatc aactccagag cggctgtcgc
     1921 agagttgaga caaacagtct taggtgtctg tgtgtaaaga gacaagcggc gctccagttc
     1981 ttttgtttta ggacctgttg tgatccaacc agaacgcagg gtatccgcta cttcagcaat
     2041 ttctgcttct gtgatatcag gcggtgaaaa tggaatattg taatttggca ttgatttgct
     2101 ccttttatct gtactcattc tcttatgact actttatttt agtaccttaa acacggtttg
     2161 aaacatgatt ttgatgtccc caaagaaact aaactctcgg agataggcga ggttatagcg
     2221 catcttttca ggaagaacat gctccacata ggcctgatca actgacagac ctttctctgt
     2281 catttgactg atgatggtgt cctcatcctt gtagttgatg ctggctggag aggtaattcc
     2341 tgcttgcaag agcaaggttg ccatcatttc agtgctatac tgctctgtat aacgtggcac
     2401 ttcaggtcgt gtaccgacaa aggacatctc acctttaagg acattgacca actgaggcag
     2461 ttcgtccaaa cggacacgtc ggatgaaatt tccaaccttg gtaatacggc tatcgttagc
     2521 agaagtcacc agatttcctt ttttatccgc atccgtcacc atggtacgga acttccaaat
     2581 cttgaaccga cggttgtact gggtcacacg ctcttgcttg taaatcactg gccccttgct
     2641 atccaacttg atccaaatgc tcaagatgag aaagatggga gaggtcagaa ccagtaagac
     2701 caaggccaaa acccagtcca agcaacgctt gaaaatcagc gaacccttcc ttttagagac
     2761 aagctggtag taagactcaa cctcgcttga tttcatttcc acaggcaagt cttcccattt
     2821 cagcatgctg ttctcctttg tttttattat actatttgtc aacacttttt gtcaacactt
     2881 ttcattatac cacaaaatgg aaaaggcggt gaaaaaaatc tgtaatttga aggatttcct
     2941 tctagtactc aatgaaaatc aaagtacagc tttgagattg cagataaagc tgacatggtt
     3001 taaagagatt ttcaaagagt ataggccatg gcccctgcct gcaaactata catcttgtga
     3061 taggttcctc ccagagccaa gagttcctca tgggttccac tctcgataat gcgtccctta
     3121 tccaagacat agatgcagtt ggcgtcttga atagtagaaa ggcggtgagc tatagcaata
     3181 gttgttcggc cctgtctcat cttcgccaga gaagcttgaa ccaagctttc tgtttcagag
     3241 tcaatattgg ctgtcgcttc atccaaaatc aggattttag gctggctggc gactgttcta
     3301 gcaaaggcaa gaagctggcg ttgcccagta gagaagctcg aaccacgctc ggaaacaggg
     3361 gagtcgtacc cctgaggaag ttcttgaata aaggaatctg catccacaaa ggctgccgca
     3421 gcctgaacct gctcatcact gatttcttgg tacatggcga tattggactt aatagttcca
     3481 tgatagagga agggttcctg caagaccaaa ccgatgtttt ttctcagctc ttcttgactg
     3541 aaatccctga tatccacatc atccaagaga actctccctg actggaattc ataaaagcgc
     3601 atgaggacat tgataataga cgatttccct gaacctgtat gacctacaaa ggcaatggtt
     3661 tcacccttat taacagagaa agaaatgtca tccagaatcg gatgtttacc gtcatatgag
     3721 aaacacacat gttcaaagcg gatattgcct tcttggactt tggcttgccc attttcttga
     3781 agaggttcat aggtcctctc gtctaccagg gcaaagacac gacctgcaga aaccatagac
     3841 gtttgcagtg ttgaaaagtt ttgcgtcacc tcaatcaaag ggtcaaagag gcggttgatg
     3901 tactggataa aagcatacat ggtcccggcc gttatcccga tagaaagacc acgatagcca
     3961 aaataggcca tcaagacagc ataacctaga agtttcaaca aactcatggc aggtctcaaa
     4021 aagagggcat ccaaggctac agaacggttg gcatagacca agtgctcttg gttgatttca
     4081 tcaaattctg cctgcaggcg cttctcttga ttaaaggcct ggattattct gattccctcg
     4141 atgttttcag ccagcttgct attgatatct gacaagagac ttctggtttt ctcaatgatt
     4201 ttcactgatt ttttccgata gagattgacc aaaaggaaaa tcaaggggag aaagagcaag
     4261 actaatgctg tcaaacgaaa atccaatacc aacatggtat aaagggttgt cagaaagatg
     4321 aaaactgctg agataaagct agataaaatc ccagaaaaca tatcactaat cgtctcggta
     4381 tcgttggtca aacgagaaac gatagaacct gctggcgtct tgtcaaagta agacatgccc
     4441 agtttctcca tattggcaaa ggcatcccga cgaatatccc taacaatact gtaagacacg
     4501 cgcgcaaaga gaagattgcc gacatactga actacagttt gtaggatgta gagaccatag
     4561 tagaccagca aaacggtaac ggctagttgg ttaagattgc tgagatactg gtcgataaag
     4621 tgggaagcca cgaggggtat gacactttta atgaccgtcg tcgctaggag aaaactgagt
     4681 gccaaaaagg tcaggagtct ataaggcttg agataagaca tcaagcgctt caatacagcc
     4741 cattgttctt gtttattctg catcttcttc tcctttcatt tctaactgct gagactggta
     4801 ggtttgggca taccagccat ccatcgctag caagtcttcg tgcctgcctc gttcgataat
     4861 ttgaccattt tgtagaacta aaataaaatc tgcatggaca acagcactga ggcgatgggc
     4921 agttataatg gttgtcttgt cctttcgcgt ctccttgaga ttgtcgataa tcgcatactc
     4981 tgtcttggca tctacggcgg ataaggaatc atccaaaatc aagatatcag ggtttaaaat
     5041 catagcccga ctcatagcca accgttgctt ttgaccacca gaaagactga ctcctttttc
     5101 accaatcagc gtatcaaatc cttgaggcat gtctacaata tcttggtaaa ctcgggctag
     5161 cttagtagct tcctcaacct ctgaaagggg caagttagga ttgccaaagc ggatattgtc
     5221 taggattgaa gtcgcaaaga gaaactggtc ctgaggaaca tagcccatga gactgcgaag
     5281 ttctgtcaaa cgatagtccc gaatatcgta accgtttaga taaatggcac ccttatccac
     5341 atcgtattca cgcaagagga gcttgattaa ggacgttttc ccagagcctg tctgcccaac
     5401 caagcccagt gtttgccctt ttgccaaact aaagtgaata tccgtcagtg tttcctcatt
     5461 ttcaaaagca aagctgtcaa tggcatactc caaacgccca ttttcaatac catccagagg
     5521 aaactcaggg tcttgtacag gagattcctg agacaaaaga ttttcaatcc gctggtaaga
     5581 aaccttccct cgctgagtag tattaaagag gaagccgatg gccataagag gccagaccag
     5641 catatccaaa tagctgataa aggtgactag attaccaact gtaatctgcc cttcctgaac
     5701 catcaaggag ccaaccaaaa gcgttaaaac ataggacgaa ccaacaaaca agagaaccat
     5761 agggtcaaag agactatcat atttcatggt ttgcaggttc ttttggaagg ttaattcatt
     5821 gactgcctga aaagacttca actcgtctgc ctgataaccg aaagacttgg tcactttgat
     5881 acctgatacg gactcctgta ccttgttatt gagttcagaa aaagcagctt gggattcgcc
     5941 aaaggcctta tgagtctttc tccctaggcg actagtcgca taggccatga aaggtagggg
     6001 gagaatggca acaagagtca tctgccatga gatgctaaag agcatggtca acaaagtcac
     6061 cagagccgtg atagaggcat ccaccgcaga catgacaccg ccacctgcta aacgagtcaa
     6121 ggcattgata tcattggttg cgtgtgccat cagatcaccc gtccgatagg tttgataaaa
     6181 ggctggcgac atttttgtga aatgcttaaa caagcgagac cgcatgatct gtcccaagca
     6241 ataagaggtc ccaaggatat acatacgcca cacatagcgc aaatagtaca taccaaaggc
     6301 tgcaagtagc aagtaaaata ggctaagaag gaggtcctgc tgggttaatt gccccgatgt
     6361 gatggcatca atgactcgcc ccataaccat aggaggaatg agattgagaa cggaaaccaa
     6421 gaccagggcc acaatcccga ctagataacg gtgtttttct aacttgaaaa accaccagag
     6481 tttttgaata atggacataa aatccctttc tgattgcaaa tggaaacctg aggc
//
LOCUS       DAVBCW010000105         6516 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVBCW010000105 DAVBCW010000000
VERSION     DAVBCW010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6516
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.105"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(76..2508)
                     /locus_tag="VQ514_001750"
     CDS             complement(76..2508)
                     /locus_tag="VQ514_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET2500482.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFMELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALVLGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     ALRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKVKYVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            complement(2510..2968)
                     /locus_tag="VQ514_001751"
     CDS             complement(2510..2968)
                     /locus_tag="VQ514_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HET2500483.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQVIQEVDRKGK"
     gene            complement(3087..3815)
                     /locus_tag="VQ514_001752"
     CDS             complement(3087..3815)
                     /locus_tag="VQ514_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2500484.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            complement(3815..4822)
                     /locus_tag="VQ514_001753"
     CDS             complement(3815..4822)
                     /locus_tag="VQ514_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET2500485.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            complement(4860..5618)
                     /locus_tag="VQ514_001754"
     CDS             complement(4860..5618)
                     /locus_tag="VQ514_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2500486.1"
                     /translation="MRNLKSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     NPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRT"
     gene            complement(5581..5871)
                     /locus_tag="VQ514_001755"
     CDS             complement(5581..5871)
                     /locus_tag="VQ514_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HET2500487.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     regulatory      complement(5942..6044)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(6127..>6516)
                     /gene="cbpD"
                     /locus_tag="VQ514_001756"
     CDS             complement(6127..>6516)
                     /gene="cbpD"
                     /locus_tag="VQ514_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HET2500488.1"
                     /translation="RRYIQLEGVTSSQNYQNQSGNISSYGSNNSSTVGWKKINGSWYH
                     FKSNGSKSTGWLKDGSSWYYLKLSGEMQTGWLKENGSWYYLGSSGAMKTGWYQVSGKW
                     YYSYSSGALAVNTTVDGYRVNSDGERV"
BASE COUNT         1796 a         1602 c         1194 g         1924 t
ORIGIN      
        1 cctagtttgc tctttgattt tcattgagtt tcactccact gtgacttatg gtcagatgct
       61 gatactttta ttcttttatg caatatcaaa ttttaactgg cctgctttga caccaatctt
      121 aagtgtgctg cctgccacta aatctccctt aagaagaagt tctgccaact tgtcctccac
      181 ttctgtttgc agggttctgc gaagtgggcg agctcccatc tctgggtcat atccttgatt
      241 tgctaacaat ttcagagctg aagcttgtaa tttcaagtca atgccttttt cagccaaact
      301 tgccactaaa ggcttgacca taatcttcac cacttcctgc atatggtcac tagataggct
      361 atggaagacc accttctcat caatacggtt gatgaattcc ggtctataag cttttttcag
      421 ttcttcaaac atgcgttttt ccatattttc ctggtcaaaa cgaatatcct tagccccaaa
      481 accaacagtc ttatcatcac gaagggcagt cgcacctagg ttcgatgtca taatgataat
      541 ggtatttgaa aaatcgacct tgcgtccctt gctatctgtc aaaacaccgt catccagaac
      601 ctgcaagaga acattaaaaa tatctgggtg ggccttctct acctcatcaa agaggagaac
      661 ggaatagggt ttattgcgaa ccttctctgt caactctcca ccttcttcgt aacccacata
      721 gcctggagga gctccattta gacggctggc tgcgaatttc tccatatact cactcatatc
      781 aaagcggata agggctgatt cgtcatcaaa gagaacttct gccaaagcct tggccaactc
      841 ggtcttaccg acacctgtgg gtcctaggaa cataaaggaa ccgatcggac gcttgtgact
      901 gcgaattcct gactgattac ggcgaatcgc acgactaatg cttgaaacag cttgatcttg
      961 accgatgaca cgtttatgga gttctgcttc aagatttaga tacttcttag catccgtttg
     1021 agtcagtttt tgaactggga ttcctgacaa gcgactcaag gtggtcaaaa tatcagactc
     1081 tgtcaccaag tctttgtaga caggtacttc ctcttctttt gcgattagct gggctgcctg
     1141 tttccacttg ccatccatca gggccttatc agctggactc aaatctgaat cgtctgcttt
     1201 tacatacttt accttatttt gcactgttgc tgctgcttcg tccaagaggt cgatagcaga
     1261 gtctggcaag tgacgactgg ttaaataacg atgagccatc ttaaccgctg tttcaaccgc
     1321 ttcatctgtg atttgtacac ggtgatgttt ctcataagtc gccttcaaac cttgtaaaat
     1381 agtcatacta tctgccacac ttggttcttc aatcgtcact ttagcgaaac gacgagaaag
     1441 agccgcatct ttttcgatat gtttttgata ttcttcctga gtggtggcac caaccgttct
     1501 caaagctcca cgcgccaagg ctggtttcaa gatattggcc gcatccagag tcgaatcaat
     1561 cccgctacca gaacccatga tggtgtggag ttcatcgata aagaggatga cttggccatc
     1621 ttcttcaata tccttgatga tattattcat gcgttcttca aagtcaccac ggaagcgtgt
     1681 ccctgcaacg acattcatca aatcaagttc taacacgcgc atcttagcca tttccgcagg
     1741 cacatcacca ctggcaatac gctgagcaag accaagcacc agagctgttt tcccgacacc
     1801 agcatcccca accaagacag ggttgttctt agtcttacgg ctcaagattt gaatcatacg
     1861 tgagatttcc ttgtcccgac cgatgactgg ttctaacttg ccagaacgcg cttgctctgt
     1921 caaatcatgc gtatagtcct cgagaccacc actaggagtc tgcggcatgc ccatcatatt
     1981 ggccatagaa ttttgtttgt cagccacagt acgatggcgt tggcgtaaag ccttgagatc
     2041 ttcacgagtc cagcctgccc gttcttctaa atttcgacga agagcagcaa tcttgacctg
     2101 atctttcttg tcttcataag aaaaaccagc cctctccaag atacgagtcg ccaaggcatt
     2161 gccatcatgc aaaatcgcat agaggacgtg ctctgtccct agtaccttag catggaccac
     2221 tgacgctaca tactctgctt catcaaaaag aacctgcaaa cgacgggaga acggcaattc
     2281 cataaaagtt tcatcctggc tatagtccgt ttcagtcagt tccaaagcca cctcttctaa
     2341 acggtccatc tcatacggat aatcatttaa agttgcccct gctacactat aactgtgatt
     2401 agacatggca atcaacaagt gccacgactc tagataacga gctccaaaat gtccagcaac
     2461 catgtaggca ctttcgatac attcattcaa tgcttttgaa tagttcatct tacttccctt
     2521 ttctatctac ctcttgtatg acctgacgta gcatgtttgc tcgaacaact ggagcttctt
     2581 ctcctaaaac gcgatccaaa gctactgatt ctagcaaatt catctcctgc ttggtcatca
     2641 attcctgctc aaccaaaagc tggagaatat cctcataaat ttcttgactg actcgctcac
     2701 caatcgagta aagcagctcc cggagcattt catgatgact agaaaactca atccgtccta
     2761 tacgaatgta gcctccgcca ccacgcttac tttcaaccaa gtagcctcta ctttccgtaa
     2821 agcgtgtctt gatcacgtag ttaatctgac taggaacaac ctgaaaggta tctgccaact
     2881 gactccgttg caactccacg ataccagatt gatctaaaat cgccttgatg taggcctcaa
     2941 tatgatccga tgtattttta aatctcataa caaacccacc tctctcttta aaccttgacc
     3001 ttgtttgact attataccga aattttctca tttttaaaag aaaaagggcg ctggtaaagg
     3061 ataatcttca ccaactccct atttttctac ttatctaagc ctaattctgc caagatttga
     3121 cgtttgtagg caatcttttg gacttccttg tcctcatctt cagaccaatc tagtttaatt
     3181 tctgaaacaa tctgcccagg gcgatttttc aagatataga tacggtcgct gagattgagg
     3241 gcctcctcaa tactatgcgt gatgatcagg gttgttagct gcaactgttt gtgaatctca
     3301 agataccaag cgtggagttc catctttgtc atctcatcca aggcgctaaa ggcctcatct
     3361 aagagaaaga gcttgtgccc aaaaaggtag gtccggagta aggctacacg ctggcgcatc
     3421 ccaccgctaa gttcatgagg atacttgtct cttacagctg tcagctggaa ggtcgcaaga
     3481 attttatccg ctcgggaaat agcttctgcc ttatccacct tttgaatcaa gaggggcaga
     3541 atgatatttc caagcaccgt cttgtgctcc aagagcagat ccttttgcaa catataactc
     3601 acgcgcccct tgggattttc ttcaccatca aggacaattc tccctgactg aacttctaaa
     3661 atcccagcga ttagattaaa gagggtggtc tttccaacac cacttgggcc tagaatggaa
     3721 accacttcgc ctgaagtcac ctgtaggttg atatcctcta aaatcctctc ctgaccatag
     3781 gcataactga catgctctag tctaatttct gtcattattt cacaaattcg ttggtgaagc
     3841 ctttgtctgt caagtcttct ttaaggatac cattttcttt atcccattta tagaaagcat
     3901 tccagcgagc tgcgtcaaat tgaccccatt tttccttgtc gcttgcgtat tcttttgaca
     3961 agtatttttg agattcgatg acaaagtcac gtttttcctt gagttcaggt gcattcttga
     4021 tgagaatatc tgcagcttct tctggatgtt ccatggcata ttggtagcct tttttgatgg
     4081 cttggatgac tttgcgagct tcttctttgt tatctttcag atagtcgttg tttgcgatga
     4141 taactggtga atagtagtca aactccttga catagtcttt caagtacatg aagttagcat
     4201 ctacaccttg agatttagca aggataccat cccaaccgta gtaaatccaa gcagtatcaa
     4261 agacgccatt ggcaatcggt gtgattgagt ttgagtcgtt atttggtact ttttcaacct
     4321 tctcaaagtc tccaccttga gattctacca aggttttcaa catagcaagt tcagttgggt
     4381 cattccatgt cccatatttc ttaccaacca agtcttttgg actgcttaca ttatcagatt
     4441 tacgagagat gattcctgat gtattgtgtt caacaatagc tgcaacggca gtgattcctg
     4501 ctcctttttc caatttctta gccatgtagt cttggaaata cactgcaaat ggtgcctttc
     4561 cgttgataac caagtcagaa gaactttctt ctggtggcaa tttcaaatca acatccactc
     4621 cagcttcttt gaaataacct ttttccttgg caacataaag ccctgtgtgg ttggtatttg
     4681 gtgtccagtc taggataaag tcaaccttct taagttctgc ctctttgttg tctttagaag
     4741 cagttccttg accacaggcc acaagcacaa cagctacaag agctgttaca agcgttaaaa
     4801 acactttcca tgttttcttc attttgattt cctcttttct ttttcaaaaa cgctctattt
     4861 taagtacgtt tccatttaat cacatatttt tcactgatat cgaccagctt catacccaaa
     4921 agactgataa tcgacaccag aataataatg gcaaacatgg tatcatactg aaacagtttt
     4981 ttagactgaa tcatataaac accaagacct tcaaaacctc ccaaccactc agataccaca
     5041 gttgtgataa aggcgtagga gacactgacc ctcagacctg cataaaagta aggcaggcta
     5101 accgggattt taaaatgcca caggatttgc caagggtttg ctcgcatcag actaaacaaa
     5161 gtcagcatat ccttgtcaca atgcctaaaa ccgtccaaaa tactaacgat gatgggaaag
     5221 gttgttgtta agataatcaa gacaatcttg ggcaaaatcc cataacccag ccacaagacc
     5281 aggataggag ctatggcaat ggtcggaatg gtctgaatga ccaccatcat agggtaaatc
     5341 aggtcattga gccaagtcaa actatccatg agcacagcca taagacaggc aatcaaaact
     5401 cccaaaatca gccccagtaa agccactctc aaggtcgccc agctatggtg ccagagaaat
     5461 tctctgtcac gaacaaaggg ctggagaatt tcaagaggtg tcggcaggat aaacttgggg
     5521 agaagtttaa gaaaacctgc taactgccag attgacaata ctccgagaaa gcccaataga
     5581 ctaatgtgtc gtctcagtat acttttcaag tttctcatca atacttaaaa tctctcctac
     5641 atttattttg acattggcaa agacattgtc tgcctcctgc cctgccactt ccagcgcttc
     5701 ttttagaatg cgcataagct catcaaactc cccttccaag accgtttcaa atggtgtcac
     5761 taccatcgtc acttcttgag tttgcagata agcaatgacc tgatcaataa cagctatccg
     5821 atcaatcccc tgtgctaggg gtaaaacttg caaggcaatg cttgctttca taagaacctc
     5881 ctcttctcgt tttcctttta aaaaaaagaa aaacctttac catacatgga aaaggtgcaa
     5941 aattagacta agtatcgttc cctccgctgg cattacccag atcaggtgcg gtcgaagttt
     6001 aacacttcct ctcagactgt acacagactc ccatatatta tatgtatata tgatagcgca
     6061 aaataagaaa aatgtcaagg aaactgctta cagaaaagag caggaaattt ctcctactcc
     6121 aattttctat actcgttctc catcactgtt tactctgtag ccatccaccg tcgtattaac
     6181 agctaaggcg cctgaagagt aagaataata ccacttacca gagacctggt accagcctgt
     6241 tttcattgcc cctgaactac ccagataata ccacgagcca ttctccttta accatcctgt
     6301 ctgcatttca ccagataatt tcaaataata ccagctagaa ccgtctttca gccatcctgt
     6361 tgatttagaa ccatttgatt tgaaatgata ccaactacca tttattttct tccaaccgac
     6421 agttgaacta ttattggatc catagctaga gatatttcct gattgattct gataattttg
     6481 tgaagaagtc actccctcta gctgtatata gcgacg
//
LOCUS       DAVBCW010000106         6489 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVBCW010000106 DAVBCW010000000
VERSION     DAVBCW010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6489
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.106"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            <1..211
                     /locus_tag="VQ514_001757"
     CDS             <1..211
                     /locus_tag="VQ514_001757"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500489.1"
                     /translation="PNPSNRRDDRVELSHQVIDTFFRLVNEPLVMFDDFITRDKTSMR
                     YQTHYQLKQKAVVRSFRKSSSDWRK"
     gene            298..2064
                     /gene="patB"
                     /locus_tag="VQ514_001758"
     CDS             298..2064
                     /gene="patB"
                     /locus_tag="VQ514_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HET2500490.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTHVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            2203..2892
                     /locus_tag="VQ514_001759"
     CDS             2203..2892
                     /locus_tag="VQ514_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET2500491.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLDEL"
     gene            2896..3075
                     /locus_tag="VQ514_001760"
     CDS             2896..3075
                     /locus_tag="VQ514_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500492.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            3062..4411
                     /locus_tag="VQ514_001761"
     CDS             3062..4411
                     /locus_tag="VQ514_001761"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HET2500493.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            4535..5995
                     /locus_tag="VQ514_001762"
     CDS             4535..5995
                     /locus_tag="VQ514_001762"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HET2500494.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDKFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENMLKEISK"
     gene            6009..6443
                     /locus_tag="VQ514_001763"
     CDS             6009..6443
                     /locus_tag="VQ514_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET2500495.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAEDGQIFTGF
                     CMEGTCGVFHLCAERAALFNTYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNER"
BASE COUNT         2031 a         1250 c         1396 g         1812 t
ORIGIN      
        1 tcccaatccc agtaaccgtc gtgatgaccg agttgaactc agccatcaag tcattgacac
       61 atttttccgc cttgtcaatg agcctcttgt aatgtttgat gatttcatta cacgagataa
      121 aacgtctatg cgttatcaaa ctcattacca attaaaacaa aaagctgtgg ttagatcctt
      181 tcggaaatcg tcaagcgatt ggaggaaatg aactaatcca cagcggctta ttccaagtat
      241 accacttggg ctttggcagt agctaactgc gctaaatata atataaggag gagtaaaatg
      301 aagacagttc aatttttttg gcattatttt aaggtctaca agttctcatt tgtagttgtc
      361 atcctgatga ttgttctggc gacttttgcc caagccctct ttccagtctt ttctggacaa
      421 gcggtgacgc agctagccaa tttagttcaa gcttatcaaa atggcaatcc agaacttgta
      481 tggcaaagcc tatcaggaat catggtcaat cttggcctgc tggttttggt tctatttatc
      541 tctagtgtaa tatacatgtg tctcatgacg cacgtgattg cagaatcgac caacgagatg
      601 cgcaaaggcc tctttggtaa gcttgctcag ttgacggttt ctttctttga ccgtcgacaa
      661 gatggcgata tcttgtctca ttttaccagt gatttggata atatcctcca agcctttaac
      721 gaaagcttga ttcaggtcat gagcaatatt gttttataca ttggtctgat tcttgtcatg
      781 ttttcgagaa atgtgacgct ggctctcatc accattgcca gcaccccatt ggctttcctt
      841 atgctgattt tcatcgtgaa aatggcacgc aaatacacca acctccagca gaaagaggta
      901 gggaagctca acgcctatat ggatgagagc atctcaggcc aaaaagccgt gattgtgcaa
      961 ggaattcaag aggatatgat ggcaggattt cttgaacaaa atgagcgcgt gcgcaaggca
     1021 acctttaaag gaagaatgtt ctcaggaatt cttttccctg tcatgaatgg gatgagcctg
     1081 attaatacag ccatcgtcat ctttgctggt tcggctgtac ttttgaatga taagtctatt
     1141 gaaacaagta cagccctagg tttgattgtt atgtttgcac aattttcaca gcagtactac
     1201 cagcctatta tccaagttgc agcgagttgg ggaagccttc agttggcctt tactggagct
     1261 gaacgaattc aggaaatgtt tgatgcagag gaggaaatcc gacctgaaaa ggctccaacc
     1321 ttcactaagt tgcaagaaag tgttgaaatc agtcatatcg atttttcata cttgcctgat
     1381 aaacctattt tgaaagatgt cagcatttct gcccctaaag gccagatgac agcagttgtt
     1441 gggccgacag gttcaggaaa aacgactatt atgaacctca tcaatcgctt ttatgatgtt
     1501 gatgctggtg gtatttattt tgatggtaaa gacattcgtg gctatgactt agatagtctt
     1561 agaagcaagg tgggaattgt attgcaagat tcggtcttgt ttagcggaac gattcgagac
     1621 aatatccgat ttggtgtgcc agatgctagt caggaaatgg ttgaggtagc agcaaaagca
     1681 acccacattc acgactatat cgaaagtttg cctgataagt acgatactct tattgatgat
     1741 gaccagagca tcttttcaac agggcagaag caattgattt caatcgctcg aaccctgatg
     1801 acagatccag aagttctcat tctcgatgaa gcaacttcaa acgtagatac ggtgacagaa
     1861 agcaagattc agcatgccat ggaggtggtt gtagcaggta gaactagttt cgtcattgcc
     1921 caccgcttga aaaccattct caatgcagat cagattattg tccttaaaga tggagaagtc
     1981 attgaacgtg gtaaccacca tgaacttttg aagctaggtg gcttttattc agaactctat
     2041 cacaatcaat ttgttttcga ataagaaaga agttgtccta tgtgggcagc tttttcttgt
     2101 ccataaaaaa tgtttatcac agccttaaaa aaaacatatt agacgaaagt cattttgagt
     2161 gatatgatag gactatcgtt agcattcgaa aggagaggca tcatggctag aacggttgta
     2221 ggagttgctg caaatctatg tcccgtagac gcagaaggca aaatcattca ttcatctgta
     2281 tcttgtagat tcgcagagat cattcgtcaa gtcggtggtc tccctttagt cattcctgtt
     2341 ggtgatgagt cagttgtacg tgattatgtg gaaatgattg acaaactcat tttgacagga
     2401 ggccaaaatg ttcatcctca gttttatgga gagaaaaaga ccgtcgagag cgatgattac
     2461 aatctggtcc gtgacgaatt tgaattggca ctcttgaagg aagcgcttcg tcagaataaa
     2521 ccaattatgg caatctgtcg cggtgtccaa cttgtcaatg ttgcctttgg tggaaccctc
     2581 aatcaagaaa tcgaaggtca ctggcaagga ctacctttcg ggacatctca ctctattgag
     2641 acagtagaag gaagcgtggt ggctaagcta tttgggaaag aaagtcaggt caactcagtc
     2701 catcgtcaaa gcattaaaga tttggcacct aatttccgtg taactgctat tgattcaaga
     2761 gaccaaacta ttgaagcgat tgagtctatc gacgagcacc gcattatcgg tttacagtgg
     2821 catccagagt ttctggttaa tgaagaagat ggcaatttag aattatttga gtatttattg
     2881 gatgaactgt aacaattgga acatctggtc gttctttctt ttattttttc aaaattttta
     2941 gaatgtggta ttttacgcaa acgtttgaat tctgataaaa attggagaaa ttcgacaaaa
     3001 aaacttgaaa aaaacgaagg taagcgttat gatagaaaag aagaaatatt ggaggaagaa
     3061 catgtcacat attaaatttg attattcaaa agttttagac aaatttgttg caccacatga
     3121 agtggaatac atgcaatcac aagtaacagc agcagatgaa ttgatccgta aaggaactgg
     3181 tgctggtagc gacttcttgg gatggttgga ccttcctgaa aaatacgacc gcgaagaatt
     3241 tgaccgcatc ttgaaagctg ctgagcaaat caagtcagac agcgatgttt tggttgtgat
     3301 cggtattggt ggatcttacc ttggtgcaaa agcagcaatc gacttcttga atcaccattt
     3361 tgctaacttg caaacaaaag aagaacgcaa agctccacaa atcctttacg caggaaactc
     3421 aatctcatct acttaccttg ctgacttggt agagtatgtt gcagacaaag acttctcagt
     3481 aaacgtaatt tctaaatcag gtacaacaac tgaaccagcg attgctttcc gtgtctttaa
     3541 agaactcttg gttaagaaat acggtcaaga agaagctaac aaacgtatct atgcaacaac
     3601 tgaccgccaa aagggtgctg ttaaggttga agcagacgct aacggttggg gaacatttgt
     3661 tgttccagat gatatcggtg gacgcttctc agtattgaca gccgttggtt tgcttccaat
     3721 cgcagcatca ggagctgaca taaaagctct tatggaaggt gcgaatgcag ctcgcaaaga
     3781 ctacacttca gacaaaatct ctgaaaacga agcttaccaa tacgcagctg ttcgtaacat
     3841 cctttatcgt aaaggctatg caactgagat cttggtaaac tatgagccat cacttcaata
     3901 cttctcagaa tggtggaaac aattggctgg tgaatcagaa ggaaaagacc aaaaaggtat
     3961 ctacccaact tcagccaact tctcaactga cttgcactca cttggtcaat ttatccaaga
     4021 aggaactcgt atcatgtttg aaacagttgt ccgtgttgac aaacctcgta aaaacgtgct
     4081 tattcctact ttggaagaag accttgacgg acttggttac cttcaaggaa aagacgttga
     4141 ctttgtaaac aaaaaagcaa ctgacggtgt tcttcttgcc cacacagatg gtgatgtacc
     4201 aaacatgtat gtgactcttc cagagcaaga cgctttcact cttggttaca ctatctactt
     4261 cttcgaattg gcaattgccc tttcaggtta cttgaatgct atcaacccat ttgaccaacc
     4321 aggtgttgaa gcttataaac gtaacatgtt tgcccttctt ggaaaaccag gatttgaaga
     4381 attgagcaaa gaacttaacg cacgtctata atagaagaaa agagtggttt gcccactctt
     4441 tttactctct ttatccatag aaattggact cagccaagac ttgtgatata atatagaaag
     4501 caaaaaggca gacgcctaga taataggaga aactatgtca aaagatatcc gcgtacgtta
     4561 cgcaccaagt ccaacaggac tactacacat cggaaatgct cgtacagcat tgtttaatta
     4621 cttgtatgcg cgccatcatg gtggaacatt tctcatccgt atcgaagata ctgaccgtaa
     4681 acgccatgtc gaggatggtg aacgttcaca acttgaaaac cttcgctggt taggcatgga
     4741 ttgggatgaa agtccagaat cacatgagaa ttatcgccag tctgagcgtt tggacttgta
     4801 tcaaaaatac atcgaccaac tattagctga aggaaaagcc tacaaatctt acgttacaga
     4861 agaagagttg gcagctgaac gtgaacgtca agaagtagct ggcgaaacac cacgctacat
     4921 caatgaatac cttggtatga gtgaagaaga aaaagcagct tacatcgcag aacgtgaagc
     4981 agcagggatc atcccaactg ttcgtttggc tgtcaatgag tcaggtatct acaagtggca
     5041 tgatatggtc aaaggcgata tcgaatttga aggtggcaat atcggtggtg actgggttat
     5101 ccaaaagaaa gacggttacc caacttacaa ctttgccgtt gttatcgatg accacgatat
     5161 gcaaatctct catgttatcc gtggagatga ccatattgct aatacaccaa aacagcttat
     5221 ggtctatgaa gctcttggtt gggaagctcc agagttcggt cacatgacct tgattatcaa
     5281 ctctgagact ggtaagaagt tatctaaacg tgatactaat acacttcaat ttatcgaaga
     5341 ttaccgaaag aaaggttacc ttccagaagc agtctttaat tttattgctc ttcttggttg
     5401 gaacccaggt ggtgaagatg agattttctc tcgtgaagaa ctcattaaac tttttgatga
     5461 aaaccgcctc agcaagtcac cagcagcctt tgatcagaaa aaactagact ggatgagcaa
     5521 tgattatatc aagaatgcag accttgaaac catctttgaa atggcaaaac cattcttaga
     5581 ggaagcaggc cgtttgactg acaaggctga aaaattagtt gagctctata aaccacaaat
     5641 gaaatcagta gatgagatta tcccattgac agatcttttc ttctcagatt tcccagaatt
     5701 gacagaagca gagcgcgaag tcatgacggg tgaaacagtt ccaacagttc ttgaagcatt
     5761 caaagcaaaa cttgaagcga tgacagatga taaatttgtg acagaaaata tcttcccaca
     5821 aattaaagca gttcaaaaag aaacaggtat taaagggaaa aatcttttca tgcctattcg
     5881 tatcgcagtt tcaggcgaaa tgcatgggcc agaattacca gatacaattt tcttgcttgg
     5941 acgtgaaaaa tcaattcagc atatcgaaaa catgctaaaa gaaatctcta aataaagagg
     6001 atacaataat ggacatctgg gaaaagatgt acgaagaagc acagaaacta tacaatccac
     6061 atgaagtatc agactttgtt tatgccaatc atgttgtagc tgcagtagaa gcagaagatg
     6121 gacaaatatt tacaggattc tgtatggagg gaacctgtgg tgttttccat ctctgcgcag
     6181 aacgggcggc cctcttcaat acgtaccaat tttcaggaca aactaaggtt aagaaagtat
     6241 tagcctttcg agacaaacca ccttatggtg gaagttcagc catgccttgc ggtgcttgta
     6301 gagaattcct tttagagctt aatgctgaaa ataaagatgc agaattcatg atggactata
     6361 atataagaaa aacagttaaa gtcgcagaac taatccctta ttggtgggga gaagaacgtg
     6421 cttctaagtt taatgagcga tagaagagtc agtataattc tggctttttt tactataagt
     6481 tgaaaaaat
//
LOCUS       DAVBCW010000107         6485 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVBCW010000107 DAVBCW010000000
VERSION     DAVBCW010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6485
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.107"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            <1..278
                     /locus_tag="VQ514_001764"
     CDS             <1..278
                     /locus_tag="VQ514_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET2500496.1"
                     /translation="GLENPFIIIDTNHDNSGKQYIEQIRIVRQTLINRAWNEKIKQFV
                     RGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALVREIYKTLGE"
     gene            280..1311
                     /locus_tag="VQ514_001765"
     CDS             280..1311
                     /locus_tag="VQ514_001765"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET2500497.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            1350..1718
                     /locus_tag="VQ514_001766"
     CDS             1350..1718
                     /locus_tag="VQ514_001766"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HET2500498.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            1708..2811
                     /locus_tag="VQ514_001767"
     CDS             1708..2811
                     /locus_tag="VQ514_001767"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET2500499.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVRVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLALPYDLIPALTLESALVHIKTVPAGACMGYGATYQTDSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKPYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            2830..4845
                     /gene="recG"
                     /locus_tag="VQ514_001768"
     CDS             2830..4845
                     /gene="recG"
                     /locus_tag="VQ514_001768"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HET2500500.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     IKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQMLKSENRVQGSGLVLNWSQEKVTAVKVSLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            5161..6141
                     /locus_tag="VQ514_001769"
     CDS             5161..6141
                     /locus_tag="VQ514_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HET2500501.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAMEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
BASE COUNT         1844 a         1285 c         1580 g         1776 t
ORIGIN      
        1 tgggcttgga aaatcctttt atcatcattg ataccaatca tgacaattct ggtaagcagt
       61 atattgaaca gatccgaatt gtccgccaga ccttgattaa ccgtgcttgg aatgaaaaaa
      121 ttaagcagtt cgttcgtggt tttatgattg agtcttatct ggaagatggt cgacaaaatg
      181 agccagaagt atttggcaag tctatcacag acccttgcct gggttgggat aacacagaag
      241 ctcttgtcag agaaatttac aaaacgttag gagaataaga tggcatttat tgaaaaaggt
      301 caagaaatcg atatggaagt catcaaggct gaaacccaat tgtctgcgga agccttgaga
      361 ctcaaggaaa gccgtgacag ggaattggca gatattattt caggggaaga tgaccgtatt
      421 ctcttggtga ttggtccttg ctcttctgat aatgaagagg cggtcttgga atatgctcgc
      481 cgtttatccg ccttgcaaaa gaaggtagcg gacaagattt tcatggtcat gcgcgtgtat
      541 actgctaagc ctcgtaccaa tggagacggc tataaaggat tggttcacca gccagatact
      601 tctaaggctc caagcctgat taacggtttg caggctgtgc gccagttgca ttaccgcgtg
      661 attacagaga ctggtttgac aacggcagat gagatgcttt atccgtcaaa tctgatcttg
      721 gtggatgact tggtcagcta ccatgccgtt ggagctcgtt ctgtggaaga ccaagagcac
      781 cgctttgtgg cttctgggat tgatgcacca gtagggatga aaaatccaac ctcaggaaat
      841 ttgggtgtta tgtttaacgc catctatgct gctcaaaaca agcaaacctt cctttatcat
      901 gggcaggaag ttgagacatc aggtaatcct ttggcccatg ttatcctccg tggagcagtc
      961 aacgagtatg gcaattatat gccgaattac tactatgaaa acctactcca agccattgaa
     1021 cgctatgaaa ccatgggact tgaaaatcct tttatcctca ttgacaccaa ccatgataac
     1081 tcaggcaagc aatatatgga gcagattcga attgttcgcc agaccttgca gaatcgtgat
     1141 tggaatgaga aaattaaaaa gacggttcga ggatttatga ttgaatctta cctagcagat
     1201 ggtcgtcaaa accaaccaga gatctttggt tgctctatta ctgacccttg cctaggttgg
     1261 gaaaatacag aggccttggt agaagagatt tatgttacct tgacaaaata agtgaaaagg
     1321 atggagttgg ggaatctcaa ctccttttga tgagaatgat agttggacac ggaattgaca
     1381 tcgaagaatt ggcttcgata gaaagcgcag ttacacgaca tgaaggattt gctaagcgtg
     1441 tactgaccgc tcaggaaatg gagcgcttca ccagtctcaa aggacgcagg caaatagaat
     1501 atttagctgg tcgctggtcg gctaaggagg ccttttccaa ggctatggga acgggcatta
     1561 gcaagctcgg ttttcaggat ttggaagtat tgaacaatga acgtggggcg ccttatttta
     1621 gtcaggcacc attttcagga aagatttggc tgtctatcag ccacaccgat cagtttgtga
     1681 cagccagtgt cattttggag gaaaatcatg aaagctagtc cacatagacc aaccaaggct
     1741 ctgattcatc tgggagctat tcgacaaaat attcagcaaa tgggggctca tatccctcaa
     1801 ggaacgctca agttggctgt ggttaaggcc aatgcttatg gtcatggagc tgttgccgtt
     1861 gccaaggcaa ttcaagatga tgttgatggc ttttgcgttt ccaatatcga tgaagccatt
     1921 gaactcagac aagctggact cagcaagcca atcctcattt taggagtttc tgaaatcgaa
     1981 gctgttgctc tagctaaaga atatgacttc accttgacag tggctggact ggagtggatt
     2041 caagcactct tagataagga agtggaccta actggattga cagtccacct caagattgat
     2101 tcagggatgg gacggattgg ttttagagag gcaagtgagg ttgagcaggc tcaagatttg
     2161 ctccaacaac acggtgttcg tgttgaagga atctttaccc actttgctac tgctgatgag
     2221 gaatcagatg actattttaa tgcccagtta gaacggttta aaactatttt agctagtatg
     2281 aaggaagttc cagagctggt tcatgctagc aattctgcaa cgactctttg gcatgtagag
     2341 actattttca atgcggttcg tatgggagat gccatgtatg gtctcaatcc aagtggagcg
     2401 gtcttggctt tgccttatga tttgataccg gccttgacct tggagtctgc tctggttcat
     2461 atcaagacag ttccagctgg tgcttgcatg ggctatggag caacttatca aacggatagc
     2521 gagcaagtca tcgcgaccgt gccaatcggg tatgcagatg gatggacaag agacatgcaa
     2581 aatttctctg tcttggtaga tggccaagct tgcccaattg tcggcagggt ttcgatggac
     2641 caaatcacta ttcgattgcc taagccttac ccactcggaa ccaaggtaac cttgattggc
     2701 tccaatgggg ataaggaaat cactgcaact caggtagcga cctaccgcgt aaccattaac
     2761 tatgaggtgg tttgcctcct cagcgaccgt attccgagag aatattacta gaaaagaaag
     2821 gagtggagca tgaatctaca tcaacccttg catgtcttgc ctggtgtggg accaaagtca
     2881 gcagaaaaat acgccaaact aggaattgaa aacttgcaag atctcttgct ctactttcct
     2941 ttccgttatg aagacttcaa aatcaagcag gtgctggagc tggaagacgg tgagaaggca
     3001 gttctttctg gtcaggtagt gactcctgct agtgtccagt attatggttt caagcgcaat
     3061 cgcctgcgtt ttagtctcaa gcagggagag gtcgtttttg cggtgaattt ctttaaccag
     3121 ccctatctgg ctgataaaat agagttggga gcaacccttg ctgtctttgg aaaatgggac
     3181 cgcgctaagg ctagtctgac tgggatgaag gttctggctc aggtagaaga tgacctccag
     3241 cctgtctatc gtctggctca gggaatcagt caggccagtc tggttaaggt catcaagacg
     3301 gcttttgatc agggactgga cctcttgata gaagaaaatc tgccccagtc tttactagac
     3361 aaatacaaac tcatgtcccg ttgtcaggca gtccgagcta tgcattttcc aaaggatttg
     3421 gcagaataca agcaggctct tcgccgtatc aagtttgagg aactctttta tttccaaatg
     3481 cagctgcaga tgctcaagtc tgaaaataga gttcagggaa gtggtctggt tctgaattgg
     3541 tctcaggaaa aagtgacagc agttaaagta agtcttcctt ttgccctgac ccaagctcag
     3601 gaaaagagtt tgcaggaaat tttaactgat atgaagtccg accaccacat gaatcgtctc
     3661 ctacaagggg atgtggggag tggaaaaacg gtagtcgctg gcttggccat gtttgcggca
     3721 gtgacagcag gttatcaggc tgccctaatg gtaccaacag aaatcctcgc agagcaacac
     3781 tttgagagtt tacagaacct ttttcccaat ttgaaactgg ctctcttgac aggttccttg
     3841 aaagctgcag aaaagagaga ggtcttggag accattgcca agggtgaggc tgatttgatt
     3901 atcggaactc acgctctgat acaagatggg gtggagtatg ctcgtcttgg tttgattatt
     3961 atcgatgagc agcaccgttt tggtgtaggg caaaggcgta ttttacggga aaaaggtgac
     4021 aatccagatg tcctcatgat gacggcgact cccattccac ggacgcttgc catcacagcc
     4081 tttggagata tggatgtttc cattatcgac cagatgccag caggtcggaa gcctattgtg
     4141 acgcgctgga tcaaacatga gcaactacct caggtcttga cttggttaga gggggaaatt
     4201 caaaaaggtt cccaagccta tgtcatctct cctttgattg aagaatcaga agctctagat
     4261 ttgaaaaatg ccattgcctt atcagaggag ttgacgactc attttgcagg caaggcagag
     4321 gtggctcttc tacatggtag gatgaagagt gacgaaaaag accagatcat gcaggatttc
     4381 aaggagagaa agacggatat tctggtttcg acgacggtta ttgaggttgg ggtcaacgtt
     4441 cccaatgcga ctgtcatgat tatcatggat gccgatcgct tcggtctcag tcaacttcac
     4501 cagcttagag gtcgtgtcgg tcggggggac aagcagtcct acgctgttct cgttgccaat
     4561 cccaagacgg attctgggaa agaccgcatg cgtatcatga cagaaacgac caatggattt
     4621 gtccttgcgg aggaagattt gaaaatgcgt ggttctggtg agatttttgg aaccagacag
     4681 tcaggacttc cagagttcca agtggctgat attatcgaag attttccgat tttagaagaa
     4741 gcaagaaagg ttgctagcta cattagttct atagaagctt ggcaagaaga tccagagtgg
     4801 cgcatgattg cccttcatct ggaaaagaaa gaacatctgg attaagcttt ctctaaggaa
     4861 aacttaagct agtttttagg tttggctctt atactaaagt catcaaaaag aaacgaggac
     4921 tctcatatga cagtaacgat taaagtaaat taccaaacca ctttccaaaa gaaggaagca
     4981 aaaaactagt ataaacagaa gagagagcga aatgctcttt ttcgtttcta aaactacttt
     5041 cagcccatca tcctaaaagt aaagaatcta aattcacttt ctatttatcc ttctttcttg
     5101 cattgattac atagatatgc tacagttgtg gtaacgatta caaaataaaa ggagcatgct
     5161 atgaaaaatc cagctttgct agaagaaatt aagacctata gaggaaggga tgaggttccg
     5221 gaagactttg atgctttctg ggatggggaa gtgaaaaatg tttccacgct tccatcctac
     5281 cacttggagg aaagagattt ccacattcct caagtcaagt gctatgagtt aacatttgaa
     5341 ggaagcaagg aaggaaaggt ctatgcacgc attgttcttc caaagagtga ggagaaggtt
     5401 ccactaatct tccattttca tggttatatg ggacgtggct gggactgggc cgacatgctg
     5461 ggcttcaccg tagctggtta cggtgttgtt tccatggatg tgcggggcca gtcaggttac
     5521 tcacaagacg gcttgcgttc tcctttagga aataccgtga aggggcatat tatccgtggt
     5581 gctatggaag gtcgggacca cctcttttat aaggatgttt atctggatat ttaccagttg
     5641 gtcgaaattg ttgctagtct gtctcaggtt gatgagaagc gtctttctag ctatggtgcc
     5701 tcacaaggag gggctctagc tctagttgca gcagcgctca atcctcgaat tcagaaaaca
     5761 gttgccattt atcccttctt gtcagacttc agacgggtga ttgagattgg taatactagc
     5821 gaggcttacg acgaactttt ccgttatttc aagtttcacg accccttcca tgaaacagag
     5881 gaggaaatca tggcgaccct tgcctatatc gatgtcaaaa atcttgccca tcgtatccaa
     5941 ggtgaggtta agatgattac gggcttggac gacgatgttt gctatcccat tacccagttt
     6001 gcgatttata atcgtctgac ctgcgataaa acctatcgca tcatgcctga gtatgctcac
     6061 gaagccatga atgtatttgt caatgaccaa gtctacaact ggctctgtgg aagtgagatt
     6121 ccttttaaat atctaaaata aggagtcgac tctaagcaca aaatcttaaa aattacaaac
     6181 acgcatagta tcaggggatt aaaaaaactt gatactatgc gttttatcat ggaaatatag
     6241 taaaatgaaa caagaatagg acaaaacgat caggacagtc aaatcgattt ctaacaatgt
     6301 tttagaagca aatgtgtact attctagttt caatctatta tatttataga attttttgtt
     6361 gctagatttg tcaaattgct taaaataatt tttttcagaa agcaaaagcc gatacctatc
     6421 gagtagggta gttcttgcta tcgtcaggct tgtctgtagg tgttaatact tttcaaaaat
     6481 ctctt
//
LOCUS       DAVBCW010000108         6354 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVBCW010000108 DAVBCW010000000
VERSION     DAVBCW010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6354
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.108"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            129..329
                     /locus_tag="VQ514_001770"
     CDS             129..329
                     /locus_tag="VQ514_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500502.1"
                     /translation="MVASASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            354..770
                     /locus_tag="VQ514_001771"
     CDS             354..770
                     /locus_tag="VQ514_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500503.1"
                     /translation="MKAALDSLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETN
                     SVPAGVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKA
                     QNLLQETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            1001..1708
                     /gene="ccdA2"
                     /locus_tag="VQ514_001772"
     CDS             1001..1708
                     /gene="ccdA2"
                     /locus_tag="VQ514_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HET2500504.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYVMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            1719..2276
                     /gene="sdbB"
                     /locus_tag="VQ514_001773"
     CDS             1719..2276
                     /gene="sdbB"
                     /locus_tag="VQ514_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HET2500505.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIA
                     VGKDAPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            2397..3788
                     /locus_tag="VQ514_001774"
     CDS             2397..3788
                     /locus_tag="VQ514_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET2500506.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPSWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            3931..4848
                     /gene="adcAII"
                     /locus_tag="VQ514_001775"
     CDS             3931..4848
                     /gene="adcAII"
                     /locus_tag="VQ514_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HET2500507.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            4856..>6354
                     /locus_tag="VQ514_001776"
     CDS             4856..>6354
                     /locus_tag="VQ514_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET2500508.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVSYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVDGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGNHFHYIPKSDL
                     SASELAAAQAYWNGKQGSRPSSSSSHNANPAQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLSERHVESDGLVFDPAQITYRTVNGVAVPHGDHYHFIPYSQLSPLEEKLA
                     RMIAVKGQNGAVLPGMHYLKPAPKPQVQPSTEKKQTDFAVEQVVRKVGEGYVVEIAGV
                     SHYVFAKDLAKDKIDAIENLLSKKTQETHALVAKKENVAPRDQEFYDKAYNLLTQAHK
                     VLSENKGRTSDFQALDKLAERLNNESSNKVKLVDDLLAFLAPITHPERLG"
BASE COUNT         1943 a         1154 c         1339 g         1918 t
ORIGIN      
        1 aaatcgaatt ctaacaatgt tttagaagtc tatatgtact attctagttt caatatacca
       61 taactgctcc tagtcaacct gcaagtcctg tcccagctcc aagctcagct ccgactcaag
      121 atcctagagt ggtagcaagc gcttcggcta gctcaacgag cacacaagct caagagcaag
      181 ttgacaagtc tgaacttcgt gccttgagtc aagagttaga ccaacgcttg aaagctttgg
      241 caacagtatc tgatccaaaa atcgatgcaa ccaaggctgt cctcctagat gctcaaaaag
      301 ctccggaaga tagtgccttg acagagtaag gtctgcatac tgcagtagag tctgtgaagg
      361 ctgccttgga ctctctcaaa gatgtgaaag caaatgctag cgacagcaag cctgcacagg
      421 acaagaagga tgcaaaacaa ggaacggaag atagtaagga ttcagataag atgactgaaa
      481 caaactcagt tccggcagga gtgattgtgg tcagtctact tgccctccta ggcgtgattg
      541 ccttctggct gattcgccgt aagaaagagt cagaaatcca gcaattaagc acggaattga
      601 tcaaggttct aggacagcta gatgcagaaa aagcggataa aaaagtcctt gccaaagccc
      661 aaaaccttct ccaagaaacc cttgatttcg tgaaagaaga aaatggctca gcagagacag
      721 aaactaaact agtagaggag cttaaagcaa tccttgacaa actcaagtaa aacatgattt
      781 atgaaatgtc agacctaaaa gacattttcg ttcacaaaag cagtgacgag taatccttgt
      841 cgttgctttt tggctatttt gggggataag atccgccttg actagcaaaa gaaaagattg
      901 gtccaatagt gcccgatacg attttgcgta gcagaaagct tacaaataaa attgaatata
      961 gtaaaatagg attagaatta ttaagaaagt tggttcttta ttggaaacga tagtattttt
     1021 aatctctgtt tttctagcag gtgttttatc ctttttttct ccttgtattt ttcctcttct
     1081 gccagtctat gctgggattt tattggatga tcaagaaagt gcaaaaagct tttctttgtt
     1141 tgggagaaag gttctctggt caggcttgat tcgaacactt tgctttatcg ctggtatctc
     1201 tctcattttc tttattctag gttttggtgc tggttacttt ggtcatattc tctatgcaaa
     1261 ttggtttcga tatgtcatgg gagctattat tatcattttg ggtcttcacc agatggaaat
     1321 ttttcatttg aagaaattag aagttcaaaa aagttttacc tttaaaaaat cagattctaa
     1381 tcgttattgg tcagcttttt tacttggtat tacctttagc tttggttgga caccttgtat
     1441 tggtccagtt ttaagttctg ttttagcact tgcggcttct ggaggcaatg gtgcttggca
     1501 aggagcgatt tatactctca tttacactct gggcatggcc cttcctttct tggtcttggc
     1561 actagcttca ggtctagtca tgccatattt tagtaaaatc aagcgtcata tgatgctact
     1621 aaagaaaatt ggtggtttcc tcattgtttt aatgggaatt ttgttactat taggacaagt
     1681 aaatgttcta gctggaattt ttgaataaag gaggacaaat gaaaaaagta atgtttgctg
     1741 gcttaagtct cttgtcatta gttgtattga tggcctgtgg tgaggaagaa actaaaaaga
     1801 ctcaagcagc acaacagcca aaacaacaaa cgactgtaca acaaattgct gttggaaaag
     1861 atgctccaga cttcacattg caatccatgg atggcaaaga agttaagtta tctgatttta
     1921 agggtaaaaa ggtttacttg aagttttggg cttcatggtg tggtccatgc aagaaaagta
     1981 tgccagagtt gatggaacta gcggcgaaac cagatcgtga tttcgaaatt cttactgtca
     2041 ttgcaccagg aattcaaggt gaaaaaactg ttgagcaatt cccacaatgg ttccaggaac
     2101 aaggatataa ggatatccca gttctttatg ataccaaagc aaccaccttc caagcttatc
     2161 aaattcgaag cattcctaca gaatatttaa ttgatagcca aggaaagatt ggaaagattc
     2221 aatttggtgc tatcagtaat gcggatgcag aagcagcatt taaagaaatg aactagaatc
     2281 gataaaatct gattacaaga tgtagtcaga tttttttaga aaaacatttt ataaatattc
     2341 acatatctcc tatatttatg gtaaaataga attatcagtt tattttggag tcaaagatga
     2401 atatatttag aacaaagaat gttagtttag ataaaacaga gatgcatagg catttgaagt
     2461 tatgggattt gattttgctg ggtatcggag ccatggtagg gacaggcgtc tttacaatca
     2521 caggtactgc agctgcaaca cttgctggcc cagccctagt gatttcaatc gttatttctg
     2581 ccttgtgtgt gggattatca gccctctttt ttgcagaatt tgcctcgcga gtacccgcta
     2641 caggaggtgc ctatagttac ctctatgcta tcttaggaga attcccttcc tggttggctg
     2701 gttggttaac catgatggag ttcatgacag ccatatcagg cgtagcttcg ggttgggcag
     2761 cttattttaa aggcttgctc tctcaatacg ggatagcctt tcctcaggct ttaaatggta
     2821 cctttaatcc tcaagcaggg acatttgttg atttattgcc tattcttgtc ttggtcttgg
     2881 tgacttcgct tgttttacta aatgccaagg cagccttacg ctttaattcg attctagtta
     2941 ttttgaaatt ttccgcttta gctctctttg ttttagtagg aatttggaat attaagtttg
     3001 ataattggag caattttgct ccttacggtt ttggacaaat ctatggagct agcactggta
     3061 ttatggctgg tgcgtccttg atgttcttcg gttttttggg atttgaatcc atctctatgg
     3121 ctgtagatga agtcaagact cctcaaaaaa atattcctag agggattgtc ttatcgcttt
     3181 ctatcgtaac cattctctat gccttggtga cccttgtttt gactggtgtg gttcactata
     3241 gtcatttaaa tgtcgatgat gccgttgcct ttgcccttcg tagtgttggg attagttggg
     3301 cagccaacta tgtgtcatta gtggctatct tgaccttgat tacagtttgt atttcgatga
     3361 cctatgccct atcgcgtatg atttacagtt tagcacgtga cggcttagtg cctgctgcct
     3421 ttaaggaact gacgaagact agcaagatac caaagaatgc tactatcttg acaggtctag
     3481 cttcagcagt agcagcagga atgttcccac tagccagtat tgcagccttt ttaaatattt
     3541 gtaccttagc ctacttgatc atgctggctt atggtctgat tcgcttacgg aaagaaaaag
     3601 gaatgcccaa agctggggaa tttaaaacac cattggtacc tctgttacca attttatcga
     3661 tcatcatttg tttatccttc atgttacagt ataatatgaa cacctggatt gctttcctag
     3721 ttgcgttgtt agtaggaagt attatttact tcacttatgg ctataagcat tctaccatag
     3781 aagaataaag tgaaagtcta ttgagttgtt gacactcagt agatttttat atgtgaaaga
     3841 aatttcaaat tttttctaaa aatatcttga cagattaaat tttcaggagt agaatattta
     3901 ctagttaatt aaatggttaa ggagttgttc atgaagaaac aaaatttatt tttagtcctg
     3961 ttaagtgtct ttcttttgtg tttgggggct tgtggtcaaa aggaaagtca gacaggaaag
     4021 gggatgaaaa ttgtgaccag tttttatcct atctacgcta tggttaagga agtatctggt
     4081 gacttgaatg atgttcggat gattcagtca agtagtggga ttcactcctt tgaaccttcg
     4141 gcaaatgata tcgcagccat ctatgatgca gatgtctttg tttaccattc tcatacactc
     4201 gaatcttggg caggaagtct ggatccaaat ctaaaaaaat ccaaagtgaa ggtcttagag
     4261 gcttctgagg gaatgacctt ggaacgtgtc cctggactag aggatgtgga agcaggggat
     4321 ggagttgatg aaaaaacgct ctatgaccct cacacatggc tagatcctga aaaagctgga
     4381 gaagaagccc aaattatcgc tgataaactt tcagaggtgg atagtgagca taaagagact
     4441 tatcaaaaaa atgcgcaagc ctttatcaaa aaagctcagg aattgactaa gaaattccaa
     4501 ccaaaatttg aaaaagcgac tcagaaaaca tttgtaacac aacatacagc cttttcttat
     4561 ctagcgaaga gatttgggct taatcaactt ggtattgcag gtatctctcc tgaacaagaa
     4621 ccaagtccac gacaactaac agaaattcag gaatttgtta agacctataa ggttaaaacg
     4681 atttttacag aaagtaacgc ttcttcaaaa gtagctgaaa ctcttgtcaa atcaacaggt
     4741 gtgggtctta aaactctgaa tcctttagag tcagacccac aaaatgacaa gacctattta
     4801 gaaaatcttg aagaaaatat gagtattcta gcagaagaat taaagtgagg aaagaatgaa
     4861 aatcaataaa aaatatctag caggttcagt ggcagtcctt gccctaagtg tttgttccta
     4921 tgaacttggt cgtcaccaag ctggtcaggt taagaaagag tctaatcgag tttcttatat
     4981 agatggtgat caggctggtc aaaaggcaga aaatttgaca ccagatgaag tcagtaagag
     5041 agaggggatc aacgccgaac aaattgttat caagattacg gatcaaggtt atgtgacctc
     5101 tcatggagac cattatcatt actataatgg caaggttcct tatgatgcca tcatcagtga
     5161 agaacttctc atgaaagatc cgaattatca gttgaaggat tcagacattg tcaatgaaat
     5221 caagggtggc tatgtgatta aggtagacgg aaaatactat gtttacctta aagatgcagc
     5281 ccatgcggac aatattcgga caaaagaaga gattaaacgt cagaagcagg aacgcagtca
     5341 taatcacaac tcaagagcag ataatgctgt tgctgcagcc agagcccaag gacgttatac
     5401 aacggatgat gggtatatct tcaatgcatc tgatatcatt gaggacacgg gtgatgctta
     5461 tattgtccct cacggcaatc acttccacta tattcctaag agtgatttgt ctgccagtga
     5521 attagctgct gcccaagcct attggaatgg gaagcaggga tctcgtcctt cttcaagttc
     5581 tagtcataat gcaaatccag ctcaaccaag attgtcagag aaccacaatc tgactgtcac
     5641 tccaacttat catcaaaatc aaggggaaaa catttcaagc cttttacgtg aattgtatgc
     5701 taaaccctta tcagaacgcc atgtagaatc tgatggccta gtatttgatc cggcacaaat
     5761 aacatatcgt acagtcaatg gtgttgctgt accgcacgga gaccattatc actttattcc
     5821 ttattcacaa ctgtcacctt tggaagaaaa attggctcgt atgattgctg ttaagggaca
     5881 aaatggtgca gtcttgccag gaatgcatta tttgaaacca gcaccaaaac cgcaagttca
     5941 accatcaact gagaaaaaac aaactgattt tgcagtagaa caggttgtta gaaaagttgg
     6001 agaaggctat gttgtggaaa ttgctggtgt cagtcactat gtttttgcta aagacttggc
     6061 taaggataag attgatgcta ttgaaaatct cttgtctaag aaaactcaag agacacatgc
     6121 tctagttgct aaaaaagaaa atgtcgctcc tcgtgaccaa gaattttatg ataaagctta
     6181 caatctgtta acccaggctc ataaagtctt gtctgaaaat aaaggtcgta cttctgattt
     6241 ccaagcctta gacaaattag cagaacgctt gaataatgaa tcttctaata aagtaaaatt
     6301 ggtagatgat ttattggcat tcctagcacc aattacccac ccagaacgcc ttgg
//
LOCUS       DAVBCW010000109         6325 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVBCW010000109 DAVBCW010000000
VERSION     DAVBCW010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6325
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.109"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            <1..368
                     /locus_tag="VQ514_001777"
     CDS             <1..368
                     /locus_tag="VQ514_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase TrpC"
                     /protein_id="HET2500509.1"
                     /translation="IVAALSEERLKELYDYATELGLEVLVETHNLAELEVAHRLGAEI
                     IGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTGQDAERLAPYFNGILVGTA
                     LMQAENVAQRIKELQIDKG"
     gene            355..954
                     /locus_tag="VQ514_001778"
     CDS             355..954
                     /locus_tag="VQ514_001778"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HET2500510.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVAADLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQSFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            932..2155
                     /gene="trpB"
                     /locus_tag="VQ514_001779"
     CDS             932..2155
                     /gene="trpB"
                     /locus_tag="VQ514_001779"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET2500511.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGQNLPDALVACVGGGSNAIGLFHPFVEDESVAMYGAEAAGFGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            2148..2924
                     /locus_tag="VQ514_001780"
     CDS             2148..2924
                     /locus_tag="VQ514_001780"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HET2500512.1"
                     /translation="MPKTLTKKLNAIKAAGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIKQAVAYQK"
     gene            complement(2896..2997)
                     /locus_tag="VQ514_001781"
                     /pseudo
     CDS             complement(2896..2997)
                     /locus_tag="VQ514_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            3226..3366
                     /locus_tag="VQ514_001782"
     CDS             3226..3366
                     /locus_tag="VQ514_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500513.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WT"
     gene            3403..3957
                     /locus_tag="VQ514_001783"
     CDS             3403..3957
                     /locus_tag="VQ514_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500514.1"
                     /translation="MFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFPL
                     VQKNKSGYLFEYAQMNTLYLNQHSSYKNDEDSHSFPIQMELVSDEMIQEIEQATKNSN
                     IEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDIP
                     NFSELSETEDFEIIPRIEKQEGQK"
     gene            3972..5123
                     /locus_tag="VQ514_001784"
     CDS             3972..5123
                     /locus_tag="VQ514_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HET2500515.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNITIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGNRLEQEANYFASCFLLPKEEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(5175..5834)
                     /locus_tag="VQ514_001785"
     CDS             complement(5175..5834)
                     /locus_tag="VQ514_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HET2500516.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELVLGLLFLLYSWELLSLGQVVLITAG
                     LTLGIYDFRHQEYPLLVWMTFHLILIASSGWNLVMVSFLALGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFLLQKKKERLPFVPFLLLAACVIIFGKLLL
                     V"
     gene            5904..>6325
                     /locus_tag="VQ514_001786"
     CDS             5904..>6325
                     /locus_tag="VQ514_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2500517.1"
                     /translation="MTIRFEEKVSIENAQLVCQWSNSLGKVFQEQWMGPRIPFLLTLQ
                     ALEGVFSIFDEQEFVGLIQKIRLEDSNLHIGRFFINPQKQEQGLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQMVEAPVR"
BASE COUNT         2047 a         1116 c         1443 g         1719 t
ORIGIN      
        1 ttattgtggc agccttgtcc gaagaacgcc tcaaggaact gtatgactac gcgacagagc
       61 ttggtctgga agtcttagtg gagactcaca atctagctga actagaggta gcccacagac
      121 ttggtgctga gattatcggg gtcaacaacc gcaacttgac tacctttgag gtcgacttgc
      181 agaccagtgt agatttagcc ccttactttg aggaaggtcg ctattacatt tctgaatctg
      241 ccattttcac agggcaggat gcggaacgac tagccccata ctttaacgga attttggtag
      301 ggacagctct tatgcaggca gaaaatgtgg cccagagaat caaggagttg cagattgaca
      361 aaggttaaaa tttgtggact atcgaccaaa gaagcggtgg aaacagccgt ttcagcagga
      421 gccgactata tcggttttgt ctttgcacct agtaaaagac aggtgacttt agaagaggca
      481 gctgagttgg caaagcttat tcctgcagat gtgaaaaagg ttggagtatt tgtttcacca
      541 agtcgggtag aactgctgga agcgattgac aaagttggct tggacttggt tcaagttcac
      601 ggtcaggtgg cagctgattt atttgagaat ttgccttgtg ccagtattca ggctgtgcag
      661 gtagatggaa atgggcatgt ccccaattct caggcagatt atctactctt tgatgcccct
      721 gtggcaggaa gtggtcagtc ctttgattgg ggtcaactgg atacgactgg actagcacag
      781 cccttcttta tcgcaggtgg ccttaatgaa gataatgtag taaaagcaat tcaacatttt
      841 actccctatg cagtagatgt atcgagcgga gtggagacag atggacaaaa agatcatgaa
      901 aagattagaa gatttataga gagggtaaag catggcatat caggaaccaa ataaagatgg
      961 attttacgga aaattcggcg gacgttttgt cccagaaaca ttgatgacag cagttttgga
     1021 gttggagaag gcctaccgtg aaagtcaggc agacccaagt ttccaagagg aattaaacca
     1081 gcttttgcgc cagtacgtag gacgtgaaac tcctctttat tacgcaaaaa acttgaccca
     1141 gcatatcggc ggagccaaga tttatctcaa acgggaagac cttaaccata caggagccca
     1201 caagattaac aatgccttgg gacaagtttg gcttgccaaa cgcatgggta aaaagaaaat
     1261 tatcgcagaa actggtgctg gtcagcacgg tgtggcaact gcaactgctg cggccctctt
     1321 taacatggaa tgtaccatct acatgggtga ggaagatgtc aaacgccaag ccctcaatgt
     1381 cttccgtatg gagcttttgg gagctaaggt cgaggccgtg acagatggtt cgcgcgtgct
     1441 caaggatgcg gtcaatgcag cccttcgttc atgggtggct aatatagatg atacccacta
     1501 tatccttggt tctgccttgg ggcctcatcc atttccagaa attgttcgtg acttccaaag
     1561 tgtcattggt cgagaggcta aacaacagta ccgtgacttg acaggacaaa atctgccaga
     1621 tgccctagta gcctgtgttg gtggtgggtc gaatgccatc ggtctcttcc atccctttgt
     1681 agaagatgag tcagtagcca tgtatggagc tgaagcggct ggatttggtg tggatacgga
     1741 gcaccacgca gctaccttga ccaagggtcg tccaggtgtc cttcacggtt ccctcatgga
     1801 tgtgctccaa gatgcccatg gtcaaattct tgaagccttc tctatctcag caggtttgga
     1861 ctatcctggt atcggtccag agcattctca ctaccacgat attaaacgtg ccagctatgt
     1921 ccctgtgaca gacgaagaag ccttggaagg attccaactc ttgtctcgtg tggaagggat
     1981 tatcccagct ttggaatcta gccatgctat cgcctttgcg gtgaaattgg ccaaagaact
     2041 tggaccagaa aagtctatga ttgtatgcct atcaggtcgt ggggacaagg atgtggttca
     2101 agtaaaagac cgcttggaag cagatgcagc aaagaaggga gaagctcatg cctaagacac
     2161 taacaaaaaa attaaatgct attaaagcag ctggaaaagg aatttttgtt ccctatatca
     2221 tggctggtga ccacgaaaaa ggtttggatg gtcttgctga aacaattcac tttttagaag
     2281 atttgggtgt ctctgcaatt gaagtgggta ttcccttttc agaccctgtt gcagatggac
     2341 ctgttatcga agaagctggc ttgcgcagtt tagcccacgg gacctctacc caggctttag
     2401 ttgaaacctt gaaaaccatt gaaacagaga ttccactggt catcatgacc tacttcaacc
     2461 ccctctttca gtacggtgtg gagaactttg tcaaagatct ggctgataca gctgtgaagg
     2521 gcttgattat cccagacctg cctcatgaac atgccaactt tgtagaacca tttttggcag
     2581 atacagatat cgccttgatt cctttagtaa gcttgaccac aggaattgag cgccagaaag
     2641 agttgattga aggggcggag ggattcgtct atgccgttgc catcaatggg gtaacaggga
     2701 aatcaggcaa ttaccgtgca gatttggaca agcacttggc gcaacttcat caagtggccg
     2761 acatcccagt cttgacaggt tttggtgtat ctagtcaagc cgatctggaa cgcttcaatg
     2821 cggtgtcaga tggtgttatc gtcggttcga aaatagtaaa agctctccac caaggagagc
     2881 cgattcagga ctttatcaaa caagcagtag cttaccaaaa ataattaaac aagtagcaag
     2941 taagaggaaa ggcacgaaag gaagtctttc ctttttcttt tgcaggcgaa aggctagttt
     3001 tatagtgaat tgaatctgga atattacact acggttccta aaatatttta agaaagtgat
     3061 ttgaatttcc caataaattt gttcagtttt taattgattt tactatagaa gatataattt
     3121 tctaaattac tctgacacag aactttaaga aaaagctaat tattgtagta atttctttaa
     3181 gaaaatgata taattcatta aaaagtattt ggaggaggaa gaattatgtt gaatcaggat
     3241 ctctttgatt cgcttgaggc acaaaaaatt gtagatactt tgatgaaagg gcaaaaagat
     3301 tatgtagatg aacgtctaga aaaaagagag acaatgatag tgtcgaatgg ttatgcatgg
     3361 acgtgaccta atcatattga tactgcattt gcatcagcag atttgtttga gtataaatta
     3421 caattagcag gacagacttg gggatattta gaatttgaaa caaatacaga aaaatatggg
     3481 aaagtattgt taattataaa gggtaagaag cgacttacga accaatttcc tttggtacaa
     3541 aaaaataaga gtggctactt atttgaatat gctcagatga atacacttta tcttaatcaa
     3601 cattcttcct acaaaaatga tgaagatagt cattcctttc caattcagat ggagttagtt
     3661 tctgatgaaa tgattcaaga aattgaacaa gctactaaaa attcgaatat cgaaaaattt
     3721 atgattttaa cttatgaggc ggactcagaa aacaatatta tatctgtaga tgttgttatg
     3781 cctgatgcac gaactggtca gttacacttg attcaagatt tgtctgagta tattcaatca
     3841 agttcgtacc atttcgaaga agctaaatat caagatattc ctaatttttc agaattatcg
     3901 gaaacagaag attttgaaat tattccaaga atagaaaaac aagaaggtca aaagtagtaa
     3961 ggaatatgta aatgtttaat ggtcgggtat tgaaagaatt acggctgtta aatggtttaa
     4021 gtagagcaga attagctcag agaattaatt taacggaaca agccatttgg cagtttgagt
     4081 ccaacgaaac gaaacctaaa ttatcaacca aaatgcattt ggccaaccaa tttcatgttg
     4141 atttaactta ttttgaacag gaagaagaga gcattcgatt tgattcttct gtaattgcct
     4201 ttagaaatgc agacctagca acacggaaaa caatagatat tcaaactatg tatttacata
     4261 aggtagatag tttgattgat tattttgaaa gttttgtaat tatacctaat attacaattc
     4321 atgacctaag taatgtagtg agtgaatctt atcataaggg agaatccatt gaggaattgg
     4381 ctctttatgc cagggaaaaa ttaggtattt caaaagataa tcatgatttg ctttataaat
     4441 tagaacgttc aggcatctat atcgtggaac gattaattaa tggccaagct gatgcttata
     4501 gcgcatggtc aaaattggga agaccttata ttgtgttggg aacgaataaa tcatctgtac
     4561 gtcgaaattt tgacttagct catgagctag gacatattct tttacacaaa tataaagata
     4621 tgaatgaaga tggcaatcgt ttggagcaag aagcaaatta ttttgcatca tgttttttat
     4681 tgccaaaaga agagttttta gtcaaatttg aagagagggt tggcaagcgt gtcagcaatc
     4741 ctgatagtta tattttattg aagtcggatt tgaatgtttc gatacaggct ttagagtatc
     4801 gagcttttaa gttaggatta ttgactccaa agcaacattc ttacttttat cgtcaaattg
     4861 cgcaaaaagg ttacaaaatg attgaacctt tggatgatca aatttttgtt aaaaaaccaa
     4921 gcaaagtaaa gagtattctg gacgtcgttt tgagtaatca tctagtcagt ctagcgacta
     4981 taatgtctaa acaaagtatt cgtttacagt ttataagcga aatattttca gtcgaaatga
     5041 aattttttga tcagtatcaa gaagatagaa gaacagatcg atttgataac atcatccctt
     5101 tgtacaaaag aaataattta taatttaact gtgaaataat ttcatgaagg acatatggca
     5161 gaaattggat tttatcagac aagcagtagc ttaccaaaaa taatcacaca agcagcaagt
     5221 aagaggaaag gcacgaaagg aagtctttcc tttttctttt gcaggagaaa ggccagaata
     5281 cccgtcgcag aagcgaactg aatcaagatc agtaactccg ttacgctaaa gacgagagca
     5341 caagaagcta aaaagaggaa atctcctgcg cccatgcgga tatcgataaa atgagccaaa
     5401 attccaagag caaggaagga gaccatgacc agattccagc cagaggaagc tattaggatt
     5461 aggtggaaag tcatccagac cagtaaggga tattcctgat ggcgaaagtc gtagatgcct
     5521 aaggtcaaac cagcggtgat taggacgact tgccccaagg aaagcaattc ccaagagtaa
     5581 agcagaaaga ggagtcctaa gactaattca aagagggcat accagacagg atagcgaact
     5641 ttgcagtagc gacagcgaaa gcgattgaag acctgtgaga gaatcggaat caaatctaag
     5701 ggacgcaagc gagtctgaca ggaatcgcag tgactggctg aactgataat ggattgctct
     5761 ggaaaacggt caatgaccaa accaagaaag gaagcgagaa tgctcccgac gagaaaaaaa
     5821 taaaaatcaa tcatacttat ctattcgtaa aaaatgggag aaatagtata atggagaaac
     5881 atagataaga tggtgaggaa aagatgacaa ttcgttttga agaaaaggtg agtatagaaa
     5941 acgctcagct cgtatgccaa tggtccaact ccttaggcaa agtctttcaa gaacaatgga
     6001 tgggaccaag gattcctttt ctactgaccc ttcaagcctt ggaaggagtc ttttcaatct
     6061 ttgatgaaca agagtttgtg ggacttatcc agaaaatcag gctagaagac agcaatcttc
     6121 atatcgggag attttttatc aacccccaga aacaggagca aggcttaggt agccaggctt
     6181 taaggaaatt tgttagtttg gcctttgaaa atgaagatat agatagtatt tctctaaatg
     6241 tcttcgaggc aaatcaaaga gctcagaatc tttaccaaaa agaaggattt gaaatcgttc
     6301 aaatggttga agcacctgta cgaaa
//
LOCUS       DAVBCW010000110         6008 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVBCW010000110 DAVBCW010000000
VERSION     DAVBCW010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6008
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.110"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(48..797)
                     /locus_tag="VQ514_001787"
     CDS             complement(48..797)
                     /locus_tag="VQ514_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HET2500518.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            930..3266
                     /locus_tag="VQ514_001788"
     CDS             930..3266
                     /locus_tag="VQ514_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HET2500519.1"
                     /translation="MDKNKIMGLTQREIKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAVASILTSYMA
                     HSEDKNPTAQAIRQRFQGQVVYPMLSNLPFSSDRKWGAMELEGLGIVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLNY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHSYVDCSKITDEELIALAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRKSMLRALPSALMVVFSVLFVKIF
                     GTSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTEQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            3368..4045
                     /locus_tag="VQ514_001789"
     CDS             3368..4045
                     /locus_tag="VQ514_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HET2500520.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            4032..4829
                     /locus_tag="VQ514_001790"
     CDS             4032..4829
                     /locus_tag="VQ514_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HET2500521.1"
                     /translation="MLASEVIHAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            4846..5949
                     /locus_tag="VQ514_001791"
     CDS             4846..5949
                     /locus_tag="VQ514_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET2500522.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQKIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLAILDQASANELFPGLQGFDRLLYASGGARVDGQLLVTRL
                     LEASHVKLVKEKVTLTPLASGYQIGEEEFEQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLVQDMEAYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDETLLQQME
                     EATLTHYLILAEATSKSERVGIRAYTSDFSPFFGQVPDLTGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPENYPIENYVKRVKSE"
BASE COUNT         1630 a         1207 c         1470 g         1701 t
ORIGIN      
        1 tgatatataa atgaaaacct tagctgtttg gccaaggttc atttcttcta tgcaagttct
       61 tctcttttct tatctgggtt ccagatgaag cttgcgataa agctaaagat gatggttagg
      121 atagcgagaa taatagcaag gatgagggca gggatgcgga taaaccacca gagtggattt
      181 ggttttttat cattgtgcca ttgtttcgtt tcttcgtcca gacgttggaa ttctgtttga
      241 atacgattgg cgactgtttc aatgccttca tcattcattt tcttgatatc tgagatatcg
      301 attggatttc caaagttcat atcgacacgt tcacggctaa tcaagccctt caaagtcatg
      361 ggaccggtgt aggtaaccgg catgatacgg accttggcca ttttggcaat cagtgctgcg
      421 ccccccttga catcgtttga gtggcggcta ccacttggaa acatgatgag agagcggtca
      481 ctttttttga gaacgttgat aggatatttg atggctgagg cgctgggatt ttcacggtcg
      541 atgggaaagg cgccacacat acgaatccac caaccaaaga tacggttggt aaagagttct
      601 ttttttgcca taaagatgaa ctgttttggc ttggtcgcaa aggccatata aacaggatcc
      661 caccaggtac ggtgaggcgc aactaaaata taattttcat cttgattagg aattttatca
      721 gtattatgat agtgagcatt gccattgatg gaccataaga gcaatacaac taatccacgc
      781 aaataagtat aaaacatgcg atctccttcg attgttttct tgttattatt ataccttatc
      841 aaaggagggc tggcaaactt ttcccttgac tagatgcata tttgggatga aattagaatt
      901 ctgttaaaaa aaatgatata atagaattta tggataaaaa taagattatg ggattaaccc
      961 aaagagaaat caaggaaaga caggctgagg gtttggtcaa tgactttacc gcatcagcca
     1021 gtaccagcac ttggcaaatc gttaaacgaa atgtctttac cctttttaac gctttgaact
     1081 ttgccattgc tttggccctt gcctttgtgc aggcttggag caatctggtc ttctttgctg
     1141 ttatctgctt taacgctttt tctgggattg tgaccgagct acgagccaaa cacatggtgg
     1201 acaagctcaa tctcatgacc aaggaaaagg tcaaaaccat ccgtgatggt caggaagttg
     1261 ctcttaatcc tgaagaatta gtgctaggag atgtcattcg tttgtctgca ggagagcaga
     1321 ttcctagtga tgccttggtt ttggaaggct ttgcggaagt caatgaagcc atgttaacgg
     1381 gagaaagtga tttggtgcaa aaggaagttg acggcttact tttgtcagga agtttcctag
     1441 ccagtgggtc agttttatct caagttcacc atgtcggtgc agacaactat gctgccaaac
     1501 tcatgcttga ggctaagacc gttaaaccca tcaactcccg tatcatgaaa tcgctggaca
     1561 agttggctgg ttttactggg aagattatca ttccctttgg tctggctctc ttgctggaag
     1621 ccttgctttt aaaaggcctg cctctcaagt catccgttgt aaactcgtcg acagctcttt
     1681 tgggaatgtt gcctaaggga attgcccttt tgaccattac ttcgctcttg actgcagtga
     1741 ttaagttggg cttgaaaaag gtcttggtgc aggagatgta ctctgttgag accttggcgc
     1801 gcgtggatat gctctgtctg gacaagacgg gtaccatcac ccaaggaaag atgcaggtgg
     1861 aggctgttct tccactgacg gaaacttatg gtgaagaggc tgttgccagc atcctgacca
     1921 gctatatggc ccatagtgag gataaaaatc caacagctca agccattcgc cagagattcc
     1981 aaggtcaagt agtctatcct atgctctcta atcttccctt ctccagcgac cgcaagtggg
     2041 gagccatgga gttagaaggt ttgggaatag ttttcttagg ggcacctgag atgttgctgg
     2101 attctgaggt cccagaagcc agagaggcct tggagagagg atcacgtgtc ttggtcttag
     2161 ctctcagtca ggagaaatta gaccatcaca aaccacagaa accatctgat attcaggctc
     2221 tagccttgct ggaaatcttg gatcctattc gagagggagc ggcagagacg ctgaactatc
     2281 tccgttctca ggaagtgggg ctcaagatta tctctggtga caatccagtt acggtatcca
     2341 gcattgccca gaaggctggt tttgcggact atcacagcta tgtcgattgt tcaaaaatca
     2401 cggatgagga attgattgct ctggcggagg agacagcgat tttcggacgt gtttcccctc
     2461 atcaaaagaa actcattatc caaacgctga aaaaagcggg tcatacaaca gctatgactg
     2521 gggatggagt taatgatatc ctggctcttc gtgaggcgga ttgttctatc gtaatggcgg
     2581 agggagaccc ggcaacccgt cagattgcca acctggttct cttgaactca gactttaatg
     2641 atgttcctga gattcttttt gaaggtcgtc gtgtggtcaa taacattgcc cacatcgctc
     2701 cgattttctt gataaagacc atctattcct tcctgttagc agtcatctgt attgccagtg
     2761 ctttactagg tcggtcagag tggattttga ttttcccctt cattccgatc cagattacca
     2821 tgattgacca gtttgtggaa ggtttcccac cattcgttct gacttttgag cgaaatatca
     2881 aacctgttga gccaaatttc ctcagaaaat ccatgcttcg tgccctacca agtgccctca
     2941 tggtcgtgtt tagcgttctt tttgtgaaaa tctttggaac gagtcaaggt tggtctgagt
     3001 tagaaatctc aactctcctc tattatctct tggggtcaat tggtttctta tccgtattta
     3061 gagcctgcat gccattcact ctatggcgag tcctcttgat tgtctggtca gtaggaggct
     3121 tcctagccac agctctcttc ccaagaatcc aaaaactgct tgaaatttca accttgacag
     3181 aacaaacgtt gcctgtttat ggtgtcatga tgttggtctt tacagtgatt ttcatcctga
     3241 ctagtcgcta tcaagctaga aaataaagaa agactgcaat ctgtggattg cagttttttt
     3301 aggtacaaga ttgccagccg aaatgtggta taataagagg taatagagtt ttggaaagtg
     3361 agagaagatg atttcaaaga gattagaatt ggtagcttcc tttgtgtcac agggggctat
     3421 tttactagat gtgggaagtg accatgctta tctgcctatc gagttggttg agagaggcca
     3481 aatcaaaagc gctattgcag gtgaggtggt ggaaggtccc tatcagtctg cggttaaaaa
     3541 tgttgaggct cacggcctaa aggagaaaat ccaagtccgt ttagccaatg gcttggcagc
     3601 ttttgaagag actgaccaag tgtctgtcat taccattgct ggcatgggtg gtcgtttgat
     3661 tgctaggatt ttagaagaag gtttggggaa gttagctaat gtagagcgtt tgatcctcca
     3721 gcccaataat cgtgaagacg acttgcgtat ctggctacag gatcatggat tccagattgt
     3781 agcagaaagc atcttagaag aagctggaaa gttttatgag attttggtgg tggaagcagg
     3841 acaaatgaag ctatcagcca gtgatgttcg ctttggtccc ttcttgtcca aagaagtcag
     3901 tccagtattt gtccaaaaat ggcaaaaaga agctgagaag ctagagttcg ctctcggaca
     3961 aatcccagaa aaaaatctgg aagaacgtca agttctagta gataagattc aagctatcaa
     4021 ggaggtgctc catgttagca agtgaagtga ttcatgctta tgaagccttt tgccctcagg
     4081 aattttctat ggagggagac agccgtggtc tgcaaattgg cactttagac aagggtatcc
     4141 aaagggtcat ggttgccctc gatattcgtg aagagacagt ggcagaggcc attgaaaagg
     4201 gtgtggactt gattatcgtc aagcacgcgc ctatctttcg tcctatcaag gacttgcttg
     4261 ccagccgtcc gcaaaatcag atttacatcg acctgattaa gcatgacatc gcagtttatg
     4321 tcagccatac caatattgat atcgttgaaa atgggctcaa tgactggttt tgtcagatgc
     4381 taggaatcga ggagacgact tatctgcagg aaacaggtcc agaacgtgga attggacgta
     4441 ttgggaatat tcagcctcag acattttggg aattggccca acaggtcaag caagtctttg
     4501 acctagatag ccttcgaatg gtgcattatc aagagaatga tttgcagaag cctatttcaa
     4561 gagtagcaat ttgtggtgga agcgggcagt ctttctataa ggatgctttg gcaaaggggg
     4621 cagatgtcta tatcactggt gatatctact accatactgc tcaggatatg ctgtctgatg
     4681 gcttgttagc attggatcca ggtcactata tcgaagtgct ttttgtggaa aaaatcgcag
     4741 cactccttac tcaatggaaa gagaaaaaag gctgggaact agagatttta cctagtcaag
     4801 catcgaccaa tcctttccgt catatctagt tagaaagtga agacaatgaa aaaagttgcc
     4861 attattggag caggaattgt gggagcaaca gctgcctact acctctcgag agaaagtgac
     4921 ctagaggtga ccgtttttga ccatgagcaa ggtcaagcca ccaaggccgc agcaggaatt
     4981 atcagtcctt ggttttccaa acgccgtaat aaagcctggt acaagatggc gcgcttgggg
     5041 gctgattttt atgtggattt attagctgat ttagagaaat caggacaaaa aatcgacttt
     5101 taccagcgtt cgggagtctt tctcttgaaa aaggatgaat ccaagttgga agaactttat
     5161 caactggccc tccagcgcag agaagaatct cccttgatag ggcaattagc cattctggac
     5221 caagcctcag ctaatgaatt attccctggt ttgcagggat ttgaccgcct gctctatgct
     5281 tctggtggag cgagagtaga tggccaactc ttggtaactc gtctgctaga agccagtcat
     5341 gtcaagctgg tcaaagaaaa agtgactctg acaccgttag catcaggcta ccagattggt
     5401 gaagaggagt ttgagcaggt tattttggcg acgggagctt ggttggggga catgttagaa
     5461 cccttgggtt atgaagtaga tgttcgtcct caaaaaggac aactacgaga ttatcagctg
     5521 gtccaagaca tggaagctta ccctgttgtc atgccagaag gggagtggga tttgattcct
     5581 tttgcaggtg ggaaattgtc cttaggcgct acccacgaaa atgacatggg atttgatttg
     5641 acggtagatg aaaccttgct ccaacaaatg gaggaggcca ccttgactca ctatctgatt
     5701 ttggctgaag ctacttcaaa atctgagcgt gttggaatcc gtgcctacac cagtgatttc
     5761 tctcctttct ttgggcaggt gcctgactta actggtgtct atgcagccag tggactaggt
     5821 tcatcaggcc tcacaactgg tcctattatt ggttaccatt tagctcaact gatccaagac
     5881 aaggagttga ccttggaccc tgaaaactac ccaattgaaa actatgtcaa acgagtaaaa
     5941 agcgaataat actcaatgaa aatcaaagaa caaactagga aactagccgc gggttactca
     6001 aagcactg
//
LOCUS       DAVBCW010000111         5991 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVBCW010000111 DAVBCW010000000
VERSION     DAVBCW010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5991
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.111"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            516..1535
                     /locus_tag="VQ514_001792"
     CDS             516..1535
                     /locus_tag="VQ514_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic biosynthesis protein"
                     /protein_id="HET2500523.1"
                     /translation="MSNTLKQLLLFREEFIKNILNGIDVDETVVIENDFVEELENFLK
                     ENDLLDFSNSIFFQLSGDKVIVNNIYPGYLAYFNRFLTFYPKIFAIKEVGEYIDFLNF
                     EKRIADMFYCWGFNANSRVLTAEKIIEIPNHQINAKYIKKVIKMSELLFGVDSNNRIR
                     LLNNRRELIKTIFQGSVIKSAIPALAATLDTISNSGSFQISLSKQIINNLVLSGKDKV
                     TIPRIELDNVVLSRQKIIVSVSYINDFLDLRFPDKIEYILNFLEGSGFNTKVMVYFGK
                     IVEEDKNYFVNLEKPQYFDFTSILFNKLFFKELNKFDYMVIEELIPNLDEDVMMTEYI
                     KEYTE"
     gene            1548..2651
                     /locus_tag="VQ514_001793"
     CDS             1548..2651
                     /locus_tag="VQ514_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500524.1"
                     /translation="MILSKVRCYIYNSKIRGKVLYELYEFINENKQLMDAINSIKVDS
                     SNGFSIQILHLGEQQTTVLLTDFFTKTLDLFKEFRVTNKSTLEQVKNQISILNSSGPK
                     MDLTNIKTDYSIVEEKLDWQSKLSKIYPNVEFIKIEKLKLILLCKYRNQIIDLEDDKK
                     RLLLLKLLYLTSKQFCYDEFKGAKFGCMSFFSHYQGYMSRLDSLDSDKFVSVKQRIVS
                     SISKLVEQYSDHIINLDRTLVDSLSDTLENQLFDDWDYILSEITTYSELLAKSGLLYN
                     SLDYKIDNVKKEVAKLSDFHQSLFKIEGFSDFFDSWQFQRYRSLVNQVYEIFSMLQVS
                     SVERTVLCGALSTIVFSKSNFNYQAFFEYLKDT"
     gene            2680..3588
                     /locus_tag="VQ514_001794"
     CDS             2680..3588
                     /locus_tag="VQ514_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500525.1"
                     /translation="MDYVDVILDEYGSVFLIKDHIKLKINNLELYRYFSSFGMSHLQF
                     RKEELPGKLKQFDFEKNKSDSYFSKKSSLIDFIKRNYFYDIKLYSDNPKNPIESWKNK
                     TEKIKIAKSTEILWSEVANFSSISKSPYYCLLYNLKNTENYILIISKTYFSAIQKMVS
                     YNLNNYFELEDVCCKSIVSKRELIENSININKSKMKLLNVCEGSKGYTRRVFKYNNIF
                     VGQLEFHSNIIFSEIALNELSIVKLLDSVESVLSEQLFGKLNWDNLFVVLSDDFNDYL
                     DINESQKYYSNGSLRGLELGIFMGGV"
     gene            3590..5488
                     /locus_tag="VQ514_001795"
     CDS             3590..5488
                     /locus_tag="VQ514_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HET2500526.1"
                     /translation="MRIIEIVDVEDQLLQLLSAHTNIDIRAQELSTLDPDSKVDLIIY
                     LKSRRDIENINNIIIKSPNRNIIVIISAFDIIYALYFPKSTFRPCLNCLLLKLKDNNP
                     EHNLLLFLKYGTLPSKHFNLDNRLKSNLILFNEKLDPKVEEYSIEGVNTTNFTFHFSC
                     SCENSGYDMSERMADILLGEGKKLSPKNYRLKKSEHLFDFTRYLDRNFGFFHHSYDEK
                     ISEYAPLHAVEINVDAGYESGWGRTFMTEAATKSAFLESLERYCNSRNRRYKKLIRGC
                     FSDLSKKYNTLNPVDFCLSNKEYRDSSYKFHDFDSEKNYDWIFGKNITTGKICLLPSQ
                     LVFYKDFSKSEENERFVYETSNGCALGSSIEEAIFYGILEIIERDNFLLTWYLKEEVK
                     QLSWESLPSRLRIINERLKEDNCELKLFNITKEVGIPVIWALYINHNENASIRSFSAA
                     GCHINPLSAIESAIFEVITSVPTFERLIEVRNKESFIEEIQNNFSLIRDIDDHILYHA
                     HADYKNKMPFLFKNNYSEYQNEFDENRIFNTYSHATLTSDLQALIKIIEKKFGDIYVV
                     NISDKVSEKFGLYCVKVLIPGLLNMSFGHSNRRVNIERIKNYVDYRGGNVNSITLNSI
                     NHLPHPFP"
     gene            5512..>5991
                     /locus_tag="VQ514_001796"
     CDS             5512..>5991
                     /locus_tag="VQ514_001796"
                     /inference="COORDINATES: protein motif:HMM:NF025394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiopeptide-type bacteriocin biosynthesis
                     protein"
                     /protein_id="HET2500527.1"
                     /translation="MKRDWKSKHLFYHDVQQFDKLVIYLSKFMDKLVNQNQIEKYFYI
                     RYWEGGPHIRLRYLVRDTQNFETIDSMINKYAYKFWENNPPMKLLNKTDYKKIYNESI
                     DNNGKMDRKLRKNATIENIAYIPEIERYGGEEALIVSEDLFCICSQVSTALLNVVDNY
                     "
BASE COUNT         2248 a          643 c         1041 g         2059 t
ORIGIN      
        1 acatgcgtta aaagttagtt gaaccgccgt atgccgaaca gcacgtacgg tggtgtgaga
       61 ggggctagag attatcccct actcgattgt ctatagctat aggggtaaag ttattagaga
      121 ggttgctagt ttatagagac atcttgattt gtaaaaagtt aaattttaga caattatccc
      181 ctttaaaaaa tctattaaac catagctagt tgaagtaaat ttttatagaa cataatttta
      241 tttatcaaga cattgtagag ttgaggcaaa ttgaaaaaga aagtttaaaa gttgataata
      301 agtcaataag aaagctatta attctaacga gattatttga tagtaatttg ttttttcagc
      361 tactattcgg acaatgttat tataataaac acaggagaga acaccagaag gtagggacat
      421 cagaagaaat agttcaggat atagttaaca taatcatcaa agaagctcct agactcgcta
      481 atgttaattt ggaaacatat tatccagatg aatatatgtc aaatacattg aagcaattac
      541 tactttttag agaggagttc ataaaaaata ttttgaatgg gattgatgtg gatgaaacag
      601 tagtaataga aaatgatttt gtagaagagt tggagaactt tttaaaagag aatgatttgc
      661 tagatttttc aaatagcata ttttttcagt tatctggaga taaagtaata gtgaataata
      721 tttatccagg atatttagca tattttaata ggttccttac attttatccg aaaatattcg
      781 ctattaaaga agttggagaa tatattgact ttttaaattt tgaaaagaga attgcggata
      841 tgttttattg ttggggattt aatgcaaatt ctagagtgtt gacagctgaa aaaataattg
      901 aaatcccaaa tcaccaaatc aatgctaagt acataaaaaa agttataaaa atgagtgaac
      961 tcttgtttgg tgttgatagt aataacagaa ttcggttgct taacaataga agggaattga
     1021 taaaaactat tttccaaggt tctgtaatta agtcagcaat tcctgctcta gctgctactt
     1081 tggatacaat cagtaacagt ggatcatttc aaatttcttt atcaaagcag ataattaata
     1141 atttagtatt aagtggtaaa gataaagtta caattcctag gatagagtta gataatgttg
     1201 ttctttcaag acagaagatt attgtatcag tttcgtatat aaatgatttt ttggatttga
     1261 gatttccaga taaaatagaa tacattttaa attttttaga aggatctggt ttcaatacta
     1321 aagtaatggt ctactttggg aaaattgtag aagaagataa gaactatttt gttaatcttg
     1381 aaaaacctca gtattttgat tttaccagta tacttttcaa taagttattc tttaaagaat
     1441 taaacaagtt tgattacatg gttattgaag aactcattcc aaatcttgat gaagatgtaa
     1501 tgatgacaga gtatataaaa gagtatactg aataggagca atctattatg attttaagta
     1561 aagtacgttg ttacatatat aattctaaaa ttagaggtaa agttctgtat gaattatatg
     1621 aatttataaa tgaaaataag caattgatgg acgcaattaa ttcaattaaa gttgacagta
     1681 gtaatggttt tagtattcaa atattacatt taggagaaca gcaaacaact gtcttattaa
     1741 cagatttttt tactaaaact ttagacttgt ttaaggaatt tagggttaca aataagagta
     1801 ctcttgagca ggtgaaaaat caaattagca tactaaattc ttctggtcca aaaatggatt
     1861 taacgaatat aaaaacagat tactcaatag tggaagaaaa attagattgg caaagtaaat
     1921 tatctaaaat ttatccgaat gttgaattta taaaaattga aaaattaaaa ttgattttgt
     1981 tgtgtaaata tagaaatcaa attattgact tagaggatga taaaaaaaga ttgttactat
     2041 tgaaattatt atatttgact tcaaagcaat tttgctatga tgaatttaaa ggagcgaaat
     2101 ttggatgtat gtcatttttt tcacattatc aaggttatat gagcagatta gattcactcg
     2161 actcagataa atttgtttcg gttaaacaga ggattgtttc ctctatttct aaattagttg
     2221 agcaatattc agatcatatt ataaatctgg atagaacttt agttgattct ctaagtgata
     2281 cccttgagaa tcagctattt gatgattggg attatatatt aagcgaaata actacatata
     2341 gcgaattatt agcaaaatca ggattgttat ataattcgct agactataag attgataatg
     2401 taaaaaaaga agtggccaag ctaagtgact ttcaccaatc tttatttaaa attgaaggat
     2461 tttcagattt ttttgatagt tggcaatttc aaagatatag gtctctcgtg aaccaagttt
     2521 atgaaatttt ttcaatgtta caagtaagta gtgtagaaag aactgtgttg tgcggagctc
     2581 taagtactat tgtctttagt aagtcaaatt ttaactatca agcttttttt gagtatctaa
     2641 aagatacgta gaattcgatt ttaaaggagg ctgaaatata tggattatgt agatgtaata
     2701 ttggatgagt atgggagtgt ttttctgata aaggatcaca taaaactaaa aattaataat
     2761 ctagaactat atcggtattt tagtagcttt ggaatgagtc atcttcaatt tagaaaagaa
     2821 gagcttccag gaaaattaaa gcagtttgat tttgaaaaaa ataaaagtga cagttatttt
     2881 tctaagaaaa gtagtctgat tgattttatt aagagaaatt atttttatga tattaagtta
     2941 tattctgata atccgaaaaa tccgatagag tcttggaaaa ataagacaga aaaaattaag
     3001 attgctaaaa gtacagaaat tctttggagt gaagtagcaa atttttcgtc aatttctaaa
     3061 tctccttact attgcttgtt atataattta aagaatactg aaaattacat attgataatc
     3121 agtaagacat atttttcagc catccaaaaa atggtttcgt ataatctaaa taattatttt
     3181 gaattagaag atgtttgttg taaaagcata gtgagcaagc gagaattgat agaaaattca
     3241 attaatatta acaaaagcaa aatgaaactg ctaaatgttt gcgaaggtag taaaggttac
     3301 acaagaagag tatttaagta caataatatt tttgtgggac aattggaatt tcactctaac
     3361 ataatctttt cagaaattgc tttaaatgaa ttaagtattg ttaagctttt agattctgtt
     3421 gaaagtgttt tatctgagca actatttgga aaattaaatt gggataattt atttgttgtt
     3481 ttatcagatg attttaatga ttatctggat ataaatgaat ctcaaaaata ctactcaaat
     3541 ggttctttga gaggactgga gcttggaatc tttatgggag gggtttaaaa tgagaataat
     3601 tgaaattgtt gatgttgagg accaactttt acaactttta agtgcacata ccaatattga
     3661 tattagagca caagagttat caacacttga cccagattca aaagttgatt tgatcatata
     3721 cttgaaatct cgacgtgata ttgaaaacat aaataatatt attattaaat cacctaacag
     3781 gaatatcatt gttataataa gtgcttttga tattatatat gcattgtatt ttccaaaatc
     3841 aacatttaga ccttgtttga actgtctatt attaaaactt aaagataaca accctgagca
     3901 taatctatta ttattcctga aatacggaac attacctagt aaacatttta atcttgataa
     3961 taggttaaaa tcaaatttaa tactatttaa tgagaagtta gaccctaagg tagaggagta
     4021 tagtattgag ggggtaaata ctacaaattt tactttccat tttagttgta gctgtgaaaa
     4081 tagtggttac gatatgtcgg aaagaatggc agatattcta cttggtgaag gaaaaaaatt
     4141 atctcctaaa aattatcgtt taaaaaagag tgagcatcta ttcgatttta ctagatatct
     4201 agatagaaat tttggatttt ttcatcattc atatgatgaa aaaatctccg aatatgcgcc
     4261 tctccatgca gtagagatta atgttgatgc tggttacgag tcaggctggg gaaggacttt
     4321 tatgacagag gctgcaacaa aatctgcttt tttagagtca ttagagagat actgcaactc
     4381 tagaaataga cgctataaaa aattaattag agggtgtttt tctgatttat ctaaaaagta
     4441 caatacatta aatcctgttg atttttgtct ttctaataaa gaatatagag atagttccta
     4501 taaatttcat gattttgatt ctgagaaaaa ttatgattgg atatttggta aaaatataac
     4561 taccggaaaa atttgcttat tgccctcgca actagtattt tataaagact ttagtaaaag
     4621 cgaagaaaat gaaaggtttg tttatgaaac atcaaatgga tgtgctttgg ggagtagtat
     4681 tgaggaagct attttttatg ggattttaga gattattgaa agagataatt ttttacttac
     4741 atggtattta aaagaagaag taaaacaatt atcatgggaa tctttaccat ctcgcctacg
     4801 tattattaat gaaagactaa aagaagataa ctgtgagctc aaacttttta atataactaa
     4861 agaagtgggc ataccagtaa tttgggcatt gtatataaat cataatgaaa atgctagtat
     4921 tcgaagtttt tcagctgcag gctgtcatat taatccttta agtgcaattg aatcagcaat
     4981 ttttgaagta ataacttctg tgccaacttt tgaaagatta attgaagtta gaaataaaga
     5041 gtcatttatt gaggaaattc agaataattt ttctttaata cgagatattg acgatcatat
     5101 attgtatcat gctcatgcgg attataaaaa taaaatgccc tttttattta aaaataatta
     5161 tagtgaatat caaaatgaat ttgatgagaa tagaattttt aatacttatt cacatgcaac
     5221 gcttacatca gatttacagg cactaataaa aattattgaa aaaaaatttg gagatatata
     5281 tgtagtgaat ataagtgata aagtttccga aaaatttgga ctttattgtg ttaaagtgtt
     5341 gattcctgga cttcttaata tgtcatttgg acattcaaat cggcgtgtaa atatagagag
     5401 gataaaaaat tatgtagatt atagaggggg aaatgtaaat agtattacat tgaattccat
     5461 aaaccatctt ccccatccat ttccataaga ttatatataa aggaataata aatgaaaaga
     5521 gattggaaat caaagcattt attttatcat gatgttcaac aatttgataa attggtaatc
     5581 tatttatcaa aatttatgga taaattagtt aatcagaatc agattgaaaa atatttctat
     5641 attcgttatt gggaaggggg gcctcatatt cgattaagat atttggtgag ggatacacaa
     5701 aattttgaaa caattgatag tatgataaat aagtatgctt ataaattttg ggaaaataat
     5761 cctccaatga agttattaaa taagaccgat tataagaaaa tttataatga gagtattgat
     5821 aacaacggaa agatggatag aaaattgagg aaaaatgcga ctattgaaaa cattgcctat
     5881 ataccggaaa tagagagata tggcggggaa gaagctctga tagtaagcga agatctattt
     5941 tgtatctgta gtcaggtttc aactgcgtta cttaatgtgg tagataacta t
//
LOCUS       DAVBCW010000112         5938 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVBCW010000112 DAVBCW010000000
VERSION     DAVBCW010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5938
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.112"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            405..1310
                     /locus_tag="VQ514_001797"
                     /pseudo
     CDS             405..1310
                     /locus_tag="VQ514_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512061.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            1355..1795
                     /locus_tag="VQ514_001798"
     CDS             1355..1795
                     /locus_tag="VQ514_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2500528.1"
                     /translation="MNFVLSSLSEGLLWSIMAIGVYLTFRILDIADMTAEGAFPMGAA
                     IVVSQIQAGANPWLATLLALLAGMVAGLISGMLHTKMKIPALLTGIVTLTGLYSINIK
                     IMGSVPNLSLGDSATVFKQLASLGLTNEGAVFSLSLVCLLLVWF"
     gene            1804..2232
                     /locus_tag="VQ514_001799"
     CDS             1804..2232
                     /locus_tag="VQ514_001799"
                     /inference="COORDINATES: protein motif:HMM:NF014684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500529.1"
                     /translation="MKTEIGLVLRSTGDNIPMSEANGVNVNTMKIVGYMISTGLIALC
                     GSLFAQNDGFSDVTSGTGTIVVGLSSEVLIHDLTIGGRLLSIGIGAIVYRLIILNIYE
                     IPNLDQNLVRLFNAILLALVLFAPALQKRLKIRGLKLRNE"
     gene            2245..3048
                     /locus_tag="VQ514_001800"
     CDS             2245..3048
                     /locus_tag="VQ514_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2500530.1"
                     /translation="MASLLTLENIHKTFEAGTVNENHVLKGLDLEVEEGDFISVIGGN
                     GAGKSTLMNILAGNLSVDEGDLLLAGKSIKHLSVRKRAKDIARVFQDPKMGTASRLTI
                     EENMAIALRRGQKRGLGWGVKEKDRIQFQEALKELNIGLENRLKVDTQYLSGGQRQAL
                     TLVMAALVKLKLLLLDEHTAALDPKTSQMVMDLTQKIVEYHQWTTLMITHDMNHAIEY
                     GNRLIMLYQGKIVVDVKGEEKKHLTVEDLMHLFQKNSGQSLVSDELVLG"
     gene            complement(3081..3791)
                     /gene="treR"
                     /locus_tag="VQ514_001801"
     CDS             complement(3081..3791)
                     /gene="treR"
                     /locus_tag="VQ514_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HET2500531.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            3976..>5938
                     /gene="treP"
                     /locus_tag="VQ514_001802"
     CDS             3976..>5938
                     /gene="treP"
                     /locus_tag="VQ514_001802"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HET2500532.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGNDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QRVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATR"
BASE COUNT         1658 a         1104 c         1381 g         1795 t
ORIGIN      
        1 ctgaaagaat tttcgaaaaa ttttaaaaaa ttttgaaata ttattgacag gtgatgaaaa
       61 aggtggtagg ataggaagga tcaaaaaaga aagcagaaaa agaaatgaat aagagacaag
      121 ccgtaactat gaatcaaaac ttttacttta gctactttag atagtgtttg tagacatgtc
      181 accatggatg caaacagttc aagcaatttg tttgtatcta tgtggctaag atttttgatt
      241 gtggtccata tagatgaata tctaaatgga ccagctaagt tgtaacttgt taggttattt
      301 caagtacccg tttagatagt atctaggcgt ttttttgttt tatcttttct gagaaggagt
      361 ttcattatga aagttgttcg aaaatcacta gccccctctt ggtagtgggg atcctcttga
      421 cttctctgat cagtttgcat cagttgaagg cagataagaa aagagatgtc tttcgtatcg
      481 gtatttcgca gtttattacc caccagtcct tagacgctac tagagaagga tttgtggatg
      541 agctggccaa acaaggctat gttgaggggg aaaatattga gattgatttg caaaatgcac
      601 agggagaaca aagaaatcta aaaacgattt cccagcaact agcagaatcc agcgatgtcg
      661 ttctagccat cgcaaggcct tctgctcaga gcttggccaa tacgacgcaa acgacaccag
      721 ttatcttttc agctgtaaca gacccagtca gtgccaagtt ggttgagtca agagaacatc
      781 ctgggggcaa tgtaactggg acaagcgatc agtcatcaga tgccatttca acccaaatca
      841 atttgataaa gaaagtgttg ctaaaggcta aaacgattgg aatcctctat actcagagcg
      901 agcccaattc ggttgtttaa aaggatgaag ctaagcgatt tctgaaagaa aaaggcttta
      961 ccgttgttga aaaaacaatc ttggacagta ataacgtcaa ggcggcagca gagagtttga
     1021 tggcagaggt ggatatggtt tttgtcccaa ctgataatat catttcgtca accatggaaa
     1081 cagttaagca ggtttctatt aaacacaagg ttccagtatt cggtggttca acagaaatga
     1141 ttgctgttgg tggcttgtat aactacggaa ccaattatga agaattggga agacagacag
     1201 ctcgcatgct gattcgtgtt ttaaaaggtg aggaaccaga aaatatagca gttgagttgc
     1261 ctgaaaaact ggaattgcat actaatcaag aaatggcaga tgcattgtga attgatatta
     1321 gtaagttaga aggcaaggaa taaggaggat taagatgaat tttgtgttat ctagtttatc
     1381 agaaggtttg ttgtggtcga ttatggcaat tggggtctac ttgactttcc gtattttgga
     1441 tattgcggat atgactgctg agggagcctt tcctatgggg gcagcaatcg ttgtatcaca
     1501 gatacaggca ggggcaaatc cttggcttgc gactttactt gctttgctgg caggtatggt
     1561 agcaggtctt atatcaggaa tgctccacac caagatgaaa atcccagctc tcttgacagg
     1621 gattgtgacc ttgacagggc tctattcaat caatattaaa atcatgggca gtgtacccaa
     1681 tctttccttg ggagattctg caaccgtctt taaacagttg gcgagcttgg gtctgacaaa
     1741 tgaaggagct gttttctcac ttagtttagt ctgtctctta cttgtttggt tttgaccctt
     1801 ttgatgaaaa cagagattgg tttggtcttg cgttcgactg gggacaatat cccgatgagt
     1861 gaggccaatg gagtcaatgt aaacaccatg aagattgttg gttacatgat ttcaaccggt
     1921 ttgattgccc tatgtggttc cttgtttgcc caaaatgatg gattttcaga tgtgacttct
     1981 gggacaggaa ccattgttgt tggtttgagc tcggaagtct tgatacacga cttgaccatt
     2041 ggaggccgct tattatccat cggaatcggt gctattgttt accgtttgat tattttaaat
     2101 atctatgaaa ttccaaatct agatcaaaac ttggtacgtc tctttaatgc aatcttgctc
     2161 gctttggttt tatttgcacc agcattgcaa aagagattaa agattcgtgg tctgaaattg
     2221 agaaatgaat aggaggagaa agttatggca agtttgttaa cacttgaaaa tattcacaag
     2281 acatttgaag cagggacggt caatgagaac catgtcctca aaggattaga tttagaggtt
     2341 gaagagggag attttatctc tgtgattggt ggaaatggag caggaaaatc cactttgatg
     2401 aatatcttgg ctggtaatct atcggtggac gaaggggacc ttctactggc agggaaatcc
     2461 attaaacatc tgagtgtcag aaagagagcc aaggatattg cccgtgtttt tcaagatcct
     2521 aagatgggaa cagcttctcg tttgacgatt gaggagaata tggctattgc cttgagacgt
     2581 gggcaaaaga gagggcttgg ttggggcgtg aaggagaagg atagaatcca gttccaagag
     2641 gccttgaagg agttgaatat tggtcttgaa aaccgcttaa aagtagatac tcaatatctc
     2701 tcaggaggac aaagacaggc tttgacccta gtcatggcag ctctggtgaa actcaaactt
     2761 ttgcttttgg atgaacatac tgcggcactt gacccgaaaa ccagtcaaat ggtgatggac
     2821 ttgacgcaaa agattgtgga gtaccatcag tggacgactt tgatgattac ccatgatatg
     2881 aatcatgcga ttgagtacgg caatcgtctc attatgctct atcaaggcaa gatagtggtg
     2941 gatgtcaagg gagaagagaa aaagcatctg acagttgaag accttatgca tctcttccag
     3001 aaaaatagtg gccaaagtct ggtcagtgat gaattagttt tgggataaag aaaaaggctg
     3061 agatctggtg tctcagtctt ttatttcttg cgttttgcaa aatctacaaa tctaaatttt
     3121 tctaacttat ggcgactttc ggtaaactga aattggcgcc cgtcttggag atagactttt
     3181 gatttaatcg aaacgacata agggtctttg ccaatgtcca tgagaatctt gtctcggtcg
     3241 cttgagtggt caatggtgat ttccttctga gcataatcaa taaggagttt gaggccattt
     3301 tctatataag aatagataga ctgctcagca atttggcgag tgagatttgg gatgagttcc
     3361 atatccagat agtccgtatc cagaacggat accagatcat ccaccacacg ctggcggacc
     3421 accttccaaa ccatccgaaa ctctgggaaa ccggttatca gtgaggattt tttatcaata
     3481 ataatcttgt ccagactgac cacgttggtt ttagagcgca gtccaagttc tttaactagt
     3541 tcttggtagc tggttaggtt ggatacagga aaattgacgg tttcttcttt gacgacttga
     3601 gaaccttgcc ctcttatctt tttgatcaat ccttcctctt ggaggagaga cagggccttt
     3661 cggacggtat cacgactcac ttgatattgc tccataaggt cgtgctcgct aggaaggaaa
     3721 tctccgacag cgtaagtctc gttttgaatg gtttcttgga tttgcttaaa taattgttgg
     3781 tatttcttca ttttgcctgc ttaaataggt ttttagtcta gtttaatgaa cacctgtatc
     3841 tattttacca tacaagtgct agaaaattta caaaaaaaca ccctttttat accaaacaag
     3901 ttgcagacaa gtttggtatc gtttacaata tacttatcaa atcaaacttg ctttgacaag
     3961 tataaggaga atcaaatggg aaaatttgaa caagaagcca aagatctgct tcaggcaatc
     4021 ggaggcaaag aaaatgtgac tgccgtaact cactgtgcaa cacggatgcg ctttgtttta
     4081 ggaaatgata agaaggctaa tgttaaagct atcgagtcaa ttccagctgt taaaggaacc
     4141 tttacaaatg caggtcaatt tcaggtaatc attggaaatg acgtgcccat cttttataat
     4201 gattttacag ctgtttcagg tattgagggt gtttccaaag aagcagccaa gtctgcagct
     4261 aagagtaatc aaaacgtggt ccaacgtgtc atgaccactc tggcggagat ttttactccg
     4321 attattccag ccttgatagt cggaggattg atcctcggtt tccgtaatgt cttggaaggt
     4381 gtccattggt cgatgttgga tggcaagacc atcacagaat cctctcagtt ttgggcaggt
     4441 gttaatcatt tcctctggtt gcctggtgaa gctatcttcc agttcttacc agtagggatt
     4501 acttggtctg tttctcgtaa gatgggaacc agccaaattt tgggaattgt tctcggaatc
     4561 tgtttggtat cgcctcagtt gctcaatgcc tatgcggttg cttcaacgcc agcagctgat
     4621 atcgcggcaa actgggtttg gaattttggc tattttactg ttaatcgtat cggttaccaa
     4681 gcccaagtta tcccagcctt gcttgcaggt ttgagtctat cttatcttga aatcttctgg
     4741 cgcaagcata tcccagaagt catttctatg atttttgtac ctttcttgtc attgattcca
     4801 gccttgattt tggctcatac tgttttggga ccaatcggtt ggacaattgg acaaggactt
     4861 tcatcagttg tcttggcagg tttaactggt ccagttaaat ggctcttcgg tgcaattttt
     4921 ggtgccctct acgctccatt tgtcatcaca ggtctgcacc atatgaccaa tgccattgat
     4981 acacaattga ttgcggatgc tggtggcact gccctatggc caatgattgc tctttctaat
     5041 attgctcaag gctcagccgt gtttgcctat tatttcatgc atcgccatga tgagcgtgag
     5101 gctcaggttt cacttcctgc aaccatttca gcctatctcg gtgttacaga accagctctt
     5161 tttggggtta acgtaaaata tatttatcca tttgttgctg ggatgactgg ttcagccctt
     5221 gcaggcatgt tatccgttac ttttaatgta actgcggctt ctattggtat cggtggtttg
     5281 ccaggtattc tctctattca acctcaatac atgctgccat ttgcaggaac tatgctagtt
     5341 gcgattgttg ttccaatgct cttgactttc ttcttccgca aggctggtct ctttacaaaa
     5401 acagagggcg atacgaactt gcaggcagaa ttcgttgctc aagaagaagc agaatttgtg
     5461 aaccatgaac cagtagaact tacttcggta gaaattatca gcccactaac tggccaagtg
     5521 aaagaattga gtcaagcgac ggatcctatt tttgcatcag gtgtcatggg gcaaggtcta
     5581 gtcattgaac caagccaagg tgagttgacc tctccagtta atgggacagt gacggttctt
     5641 ttccctacca agcatgccat cggcattgtc tctgacgagg gagttgaatt gctcatccac
     5701 atcggtatgg atacagtagg tcttgatggc aaaggttttg aaagtcttgt agtccaagga
     5761 gatcacgtta cagttggtca gcaactgatt cgttttgata tggatgtcat taaggctgca
     5821 ggtctggtga cagaaactcc tgttatcatc accaaccaag atgcttatac agcgactatt
     5881 cccggaactt atccgacaac gattcaagct ggagcatctc tcatggtcgc tacacgaa
//
LOCUS       DAVBCW010000113         5782 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVBCW010000113 DAVBCW010000000
VERSION     DAVBCW010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5782
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.113"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            203..751
                     /locus_tag="VQ514_001803"
     CDS             203..751
                     /locus_tag="VQ514_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET2500533.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIQ"
     gene            complement(988..>5782)
                     /locus_tag="VQ514_001804"
     CDS             complement(988..>5782)
                     /locus_tag="VQ514_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET2500534.1"
                     /translation="QGERGLTGAKGEKGDQGERGLTGAQGEKGDRGERGLTGAKGEKG
                     AQGQAGRDGVTPTVTVKDNKNDGTHTITINDGRGNVTSTVVRDGFDGASPLVATQRND
                     ADKTTTVIFYYDKNGNNELDASDKKLKEVVIADGAKGEKGDKGEQGLQGRDGEQGPKG
                     EDGKTPTVKVTDGQDGTHTITINDGKGGITTTVVRDGFDGASPLVSTHRNEADKTTTV
                     IFYYDLNDNNQFDEGDTKLKEVVIADGKQGPKGDKGDNGKDGFTPEVTVTDNNNGTHT
                     ITITQPDNRPSLTTIVKNGEDGKTPKVKAERDDAKKQTTLTFYIDKDGDGSYTAGKDE
                     LVQTTVVKDGQDGAAGASGRDGKEVLNGKVDPTTEGKDGDTFVNTQTGDVFVKKGNTW
                     EPAGNIKGPKGDKGADGAKGEKGAQGERGLTGAQGVKGEKGDQGERGLTGSKGEKGDQ
                     GERGLTGAQGAKGDKGEQGLQGRDGAQGPKGADGQRGPAGPQGPKGEQGNPGTPGKDG
                     KSLIAVKNGTETKVYVEDPARPGQPLNPNQPLATITDGRNGTNGQSPTITATRSVQNG
                     KNGVLVTITPVGGRPQTTFVEDGQKGADGKTPTVTITEGQNGTHTLTVHNPGSPDVTT
                     TIRDGATGQAGRDGKDVLNGKVNPQPNQGKNGDKYINTETGDVYVKNNGNWDKEGNIK
                     GPKGDKGADGAKGEKGDQGERGLTGAQGAKGADGAVGRDGRDGKDVLNGKANPEAHQG
                     KDGDKYVNTETGDVFVKNNGNWDKEGNIKGPKGDKGERGEDGKTPEVTVTPGKDGHST
                     DITFTVPGKDPVTVNVKDGENGLNGKTPKVDLLRVQGKNGNPSHTIVTFYTDENNDGK
                     YTPGTDELLGSEMIKDGAKGADGRDGKSLLTVKDGKETKVYQEDPANPGQPLNPEKPL
                     AVIRDGVDGKSPTVTAVRKDEAGHKGVEITVDNHDGSQPTTVFVQDGAKGETGATGQD
                     GQTPTITTQRGQDGQSTVVTITTSGKDPVTFTVKDGKNGKDGRAPKIKVEDITSPSRI
                     RRDTDAAATPTRNGIRVTVYDDVNDNGVYDEGVDKVLNSKDIYNGIDGRDGSAPTITT
                     KDNGDGTHTITVQNPDGSESTTVVKDGKDGKTANITTTENPDGSHTITVTNPDGSTKE
                     TVVKNGKDGKTPKVEVTDNNDGTHTVKVTDGDGNVTNAIIKDGKDGKAATATTTENPD
                     GSHTVTITNPDGTKNEFVVKNGRDGVDGRTPTASVRDNGDGSHTIVITNPEGVTTETT
                     VRDGKSPKVTITDEQNGTHKISVLNGDGTTTTTTVRDGREPKLEVIDNNDGSHTIKVT
                     GADGKGTTTTIFDGKSPKANIVDNGDGTHTLTIVDSDGREYKSIIKDGKDGKDSVSPT
                     VTVKNNNDGTHVVTITNPDGSKTEMVIKDGKDGKSPKVSVEDNGDGSHTITIINSDGT
                     VTKTVIKDGRDGRDGRDGKDGKDGKDGKDGKCGCQDKPVTPSNDKPVPPTPNVPTPEV
                     PVKPVPAQPTPNVPTPEVPVQPTPAVPTPEVPVKPVPAVPEQPVVPTPAQPATPVNAN
                     PVAPTTGKENRGDKLPETGSQSDYISVLLGSGILLSLYVGRRKEA"
BASE COUNT         1243 a         1340 c         1045 g         2154 t
ORIGIN      
        1 attctatttc tcccttcaaa caaaaccgat tttgaaagtg aaacagttct tactttttca
       61 gtcacaaatg actagagttt ccgaaatttt acaattttca aataaactct gagcaaattt
      121 cttgcaagtt cttttgtttt gttgtaatgt attttataac aacgagagag ttctcaaaat
      181 ttgaagaaaa aggagacaca tcatgtctaa aaaagtatta tttatcgtcg gatcactacg
      241 tcaaggttct ttcaaccacc aaatggcgct cgaagctgag aaagcacttg ctggtaaagc
      301 ggaagttagc taccttgatt attcagccct tcctctcttc agccaagatt tggaagttcc
      361 aacacatcca gctgtagctg ctgctcgtga agcagttctc gttgcggatg ctatctggat
      421 tttctctcca gtctacaact tctctatccc tggtacagta aaaaacttgc ttgactggct
      481 atctcgtgcc cttgacttgt ctgatacgcg tggcgtttct gcccttcaag acaagtttgt
      541 caccgtatca tctgtagcca atgcagggca cgatcaactt ttcgctatct acaaagacct
      601 cttgccattt atccgtacac aaggcgttgg tgatttcact gctgcacgtg ttaatgactc
      661 tgcctgggca gacggaaaat tggttcttga agaaacagtc ctaaactcac ttgaaaaaca
      721 agctcaagac ttggtcgaag cgattcagta aaaaatagaa aattttagaa agtctaaatc
      781 aattatagcc acctaatagg gtggcttttt atttctcaat caagattttc agacaaacat
      841 cagccatcca aaaaaacctt gagcgttacc actcaaggtt tttagcagtc ttttccatca
      901 taggaaaatc attctaagaa tattctttca ttcgagatct atcacttatc atcttctttc
      961 gagaaattag tgactgcttt tggctgatta agcttctttt cttcgtccta catataggct
     1021 caatagaata ccgctaccta aaagaacaga gatataatca gattggcttc cagtttcagg
     1081 taatttgtcc ccacggtttt ctttacctgt agttggtgct actgggttag catttactgg
     1141 agttgctggt tgagccggtg ttggtactac tggttgttct ggaaccgctg gtactggttt
     1201 aaccggtact tctggtgttg gaacagctgg agttggttgt actggcactt ctggtgtcgg
     1261 tacatttggt gttggttgcg ctggcacagg tttaaccggt acttctggtg tcggcacatt
     1321 tggtgttgga ggaactggtt tgtcatttga tggtgttact ggtttgtctt ggcatccaca
     1381 ttttccatct ttaccgtctt taccgtcttt accgtctttg ccgtctcgac catcacgtcc
     1441 atctctacca tctttaataa ctgtttttgt cacagttcca tcagaattga tgattgtgat
     1501 tgtatgacta ccatcaccat tatcttcaac agaaactttt ggtgatttac catctttacc
     1561 gtctttaatc accatttctg tcttacttcc atctggatta gtgattgtaa caacgtgagt
     1621 tccatcgtta ttatttttaa cagttacagt tggtgaaacg ctatctttgc cgtctttacc
     1681 atctttgata atagatttgt attcacgacc atcagaatct acgattgtta atgtatgagt
     1741 tccatctccg ttatcaacga tgttcgcttt tggtgattta ccatcaaaga ttgtagttgt
     1801 cgttccttta ccatcagcac ctgtcacttt aatagtgtgt gaaccatcgt tgttatcaat
     1861 aacttcaagt tttggttctc taccatcacg aactgtagtt gttgtagttg ttccgtcacc
     1921 atttagaaca gagatcttat gagttccatt ttgttcatca gttatagtca cttttggtga
     1981 tttaccatca cgaactgtgg tttcagttgt cacaccttct ggatttgtaa taacgattgt
     2041 atgacttccg tctccattat cacgaacaga tgcggttgga gtacgtccgt caacaccgtc
     2101 acgtccattc ttaacaacaa actcattctt agttccgtct gggttagtga ttgttactgt
     2161 gtggcttcca tctggatttt cagtagttgt tgctgttgca gctttaccgt ctttaccatc
     2221 tttgatgata gcgttggtaa cattgccgtc tccatctgta actttaacag tatgagttcc
     2281 atcgttgtta tccgttactt caactttagg agtcttaccg tctttaccgt ttttaacaac
     2341 agtttcttta gttgaaccat ctggatttgt caccgtaatt gtgtggcttc catctgggtt
     2401 ttctgttgta gtgatattcg cagttttacc gtctttacca tctttaacaa ctgttgttga
     2461 ttcagaacca tctgggtttt gaactgtgat agtgtgagtt ccatctccat tatcttttgt
     2521 agtaatagtt ggagctgaac catcacgtcc atctataccg ttataaatat ctttactatt
     2581 taatacttta tcgacacctt cgtcgtatac cccattgtca ttaacatcat cataaactgt
     2641 aacacggata ccgttacgcg ttggagttgc agcagcatct gtatcgcgtc taattcttga
     2701 aggtgaagta atatcttcta ctttgatttt cggtgcacgg ccatctttac cattcttacc
     2761 atcttttaca gtgaaggtta ctggatcttt acctgatgtt gtgatagtta caacagtgct
     2821 ttggccatct tgtccacgtt gagtagtgat tgtaggagtt tgtccatcct gaccggttgc
     2881 accagtttct cccttagcac catcttgaac aaagactgta gttggttgtg aaccatcatg
     2941 gttatcaaca gtgatttcta cacctttatg ccctgcttca tctttacgaa cagctgtaac
     3001 tgtaggtgat tttccatcta ctccatctct aattaccgca agtggttttt ctggatttaa
     3061 tggttgtcct gggttagctg gatcttcttg gtaaacttta gtttctttac catccttgac
     3121 agtaagcaat gatttaccat ctcgtccgtc cgcgccttta gcaccatctt taatcatttc
     3181 tgaacctaga agttcatcag ttcctggtgt atatttgccg tcattgtttt catctgtata
     3241 gaatgtcaca attgtatgag atggatttcc gttttttcct tggacacgaa gtaaatcaac
     3301 ttttggagtt ttaccgttca gaccattttc tccgtcctta acatttactg taactggatc
     3361 tttacctgga acagtgaatg taatgtcagt actatggcca tctttacctg gagttacagt
     3421 tacttctgga gtcttaccat cttctccgcg ttcacctttg tcacccttag ggcctttgat
     3481 gttgccctct ttatcccagt tgccgttatt cttaacgaag acgtcgcctg tttctgtatt
     3541 aacgtattta tcgccgtctt taccttgatg tgcttctggg ttagctttgc cgttcaacac
     3601 gtctttaccg tcacgtccat cacgacctac tgcgccatcc gcacctttag ctccttgcgc
     3661 tccagttagg ccgcgttcgc cttggtctcc tttttcgcct ttagcaccat ctgcaccttt
     3721 gtccccttta gggcctttga tgttgccttc tttatcccag tttccattgt ttttaacata
     3781 gacatcaccg gtttcggtat taatatattt atctccattt ttaccttggt ttggttgtgg
     3841 atttactttt ccgtttaata catctttacc atcacgacct gcttgtcctg tagctccatc
     3901 acggatcgta gttgtcacat ctggacttcc tggattatga accgttaatg tatgtgtgcc
     3961 gttttgcccc tcagttattg ttactgttgg ggttttccca tcagcaccct tttgtccatc
     4021 ctctacaaat gttgtttgtg gacgacctcc tactggtgta attgttacta atacaccatt
     4081 tttgccattt tgcacacttc gtgtcgctgt aattgttggt gattgtccat tagtaccgtt
     4141 tcttccatca gtgatagttg ctaatggttg gtttggatta agtggttgtc ctggtctagc
     4201 cggatcctct acatagactt ttgtttcagt tccattttta acagcaatta gagattttcc
     4261 atctttacct ggagttcctg gattaccttg ttctcctttt ggtccttgtg gtcccgctgg
     4321 tcctctttgt ccatctgcgc cttttggtcc ttgagctcca tcacgacctt gaagaccttg
     4381 ttcacctttg tcacctttgg caccttgcgc tccagttaaa ccgcgttcac cttggtctcc
     4441 tttttcacct ttagatccag ttagaccgcg ttcaccctga tctccctttt cacccttgac
     4501 accttgcgct ccagtcaggc cgcgttctcc ttgggctcct ttttcaccct tggcaccatc
     4561 tgcacctttg tcacctttcg gtcctttgat gtttcctgct ggttcccaag tgttaccttt
     4621 cttaacgaat acatctcctg tttgtgtatt tacgaatgtg tctccatctt taccttctgt
     4681 tgttgggtct acttttccgt ttaatacttc ttttccatca cgtccagatg ctcctgcagc
     4741 tccgtcttgt ccgtctttaa ctactgttgt ttgaactaac tcgtcttttc ctgctgtgta
     4801 acttccatct ccatctttgt caatgtagaa tgttaatgtt gtttgtttct tcgcatcatc
     4861 tcgttctgct ttgacttttg gtgtttttcc atcttcaccg tttttaacga ttgttgttaa
     4921 tgatggtctg ttgtctggtt gtgtgattgt gatggtgtgt gttccgttat tgttatctgt
     4981 aactgttact tctggtgtga atccatcttt tccgttatca cctttgtcac cttttggtcc
     5041 ttgttttcca tctgcgataa caacttcttt aagttttgta tctccttcat caaattgatt
     5101 attatcattt agatcataat agaaaataac agttgttgtt ttatctgctt catttctatg
     5161 agtagaaaca agagggcttg caccatcaaa tccatctctt actactgtag tagttatacc
     5221 acctttacca tcgttaattg taattgtatg cgttccatct tgaccatcag ttactttaac
     5281 tgttggggtt ttcccatctt ctccttttgg tccttgttca ccatcacgcc cttgaagacc
     5341 ttgttcacct ttgtcccctt tttcaccttt agcaccatct gcaattacaa cttcttttaa
     5401 tttcttatca gaagcatcta attcattgtt tccattttta tcgtaataga agataacagt
     5461 tgttgtctta tctgcgtcat ttcgttgagt cgcaactaat ggacttgcgc catcgaaacc
     5521 atctcttaca acagtacttg taacattacc tctaccgtcg ttaatagtga tagtatgagt
     5581 gccgtcattt ttattatctt taacggttac ggttggagtt acaccgtcac gacctgcctg
     5641 accttgggct cctttttcac ctttagctcc agttaaacca cgttcgcctc ggtctccttt
     5701 ttcaccttgg gctccagtga gaccgcgttc accttggtcc cctttttcac ctttagctcc
     5761 agttaaaccg cgttcacctt gg
//
LOCUS       DAVBCW010000114         5658 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVBCW010000114 DAVBCW010000000
VERSION     DAVBCW010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5658
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.114"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            163..1182
                     /gene="galE"
                     /locus_tag="VQ514_001805"
     CDS             163..1182
                     /gene="galE"
                     /locus_tag="VQ514_001805"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET2500535.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTATLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAIEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            1184..2137
                     /locus_tag="VQ514_001806"
     CDS             1184..2137
                     /locus_tag="VQ514_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2500536.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTSWSFRKLFFYSIE
                     GIINFSDFPLIIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDDRLKKIEFI"
     gene            2289..2954
                     /locus_tag="VQ514_001807"
     CDS             2289..2954
                     /locus_tag="VQ514_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500537.1"
                     /translation="MLSKEDYTEEIGLIKKQNYVEAELYPIVADIIKPTLKDSLSKRY
                     VFGRQRRGLGQIYYGLSNFPDIVILDKTYENKSRKSIKIEEWKKLRGCVEVKNLNYSL
                     ITEEKIKSTISNSFEHITGEMEQLTGEMGQLIGDLLWYKKVIYTNGIEWRFLSLDDKE
                     EIDNTIVEVVNKRIETEEAGNSFDWWKNIKDLSFNYTDICLSKDCRQEWNEFVKRVKE
                     IEW"
     gene            2993..3094
                     /locus_tag="VQ514_001808"
                     /pseudo
     CDS             2993..3094
                     /locus_tag="VQ514_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group 2 glycosyl transferase"
     gene            complement(3145..3351)
                     /locus_tag="VQ514_001809"
     CDS             complement(3145..3351)
                     /locus_tag="VQ514_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HET2500538.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            3403..3879
                     /locus_tag="VQ514_001810"
     CDS             3403..3879
                     /locus_tag="VQ514_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HET2500539.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            3888..4559
                     /locus_tag="VQ514_001811"
     CDS             3888..4559
                     /locus_tag="VQ514_001811"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HET2500540.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDFETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            4690..5028
                     /locus_tag="VQ514_001812"
     CDS             4690..5028
                     /locus_tag="VQ514_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HET2500541.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLADGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            5031..>5658
                     /locus_tag="VQ514_001813"
     CDS             5031..>5658
                     /locus_tag="VQ514_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HET2500542.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITKHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
BASE COUNT         1772 a          951 c         1254 g         1681 t
ORIGIN      
        1 aaccataccc atggcaaggc gacgttgacg tggtttgaat tggattttcg aagagtataa
       61 gcattttact gaaattttat caggtagttt aggatggcaa atgacattcc ctgtcaaaaa
      121 tggtaaaata agaaaaaata atctgagaat cgaggaaaaa agatgcaaga aaagattttg
      181 gtaactggtg gtgccggttt tattggaacc cacactgtta ttgaattgat ccaagcaggc
      241 catcaagttg ttgtggtgga taatcttgtc aatagcaatc gtaagagttt agaagttgtt
      301 gaaagaatca caggagttga gattcctttc tatgaggcag atattcgtga cacagctacc
      361 ctcagagata ttttcaagca ggaagaaccg actggtgtca ttcactttgc tggtttgaag
      421 gctgttggcg aatcaacacg tatccctctt gcctactatg acaacaatat cgctggaact
      481 gtcagccttt tgaaagccat agaagaaaac aactgtaaaa acatcatctt cagttcttct
      541 gcgacagttt acggggatcc gcacacagtg cccatcttgg aagatttccc actttcagtg
      601 accaacccat acggtcgtac taagctcatg ctagaggaaa ttttgactga tatttacaaa
      661 gcagactcag aatggaatgt tgtcttgctt cgttacttta acccaatcgg agcccatgag
      721 agtggtgatt tgggagaaaa tccaaacggt attccaaaca atctcttgcc atatgtgact
      781 caagtagccg ttggaaaatt agagcaagtg caagtgtttg gagacgatta cgatacggaa
      841 gatggaacag gtgttcgtga ctatatccac gttgtcgatt tggctaaggg tcacgttgca
      901 gctttgaaaa aaatccaaaa aggttcagga ctaaacgttt ataaccttgg aactggtaaa
      961 ggttactcag ttcttgaaat tatccaaaac atggaaaaag cggtgggacg tcctattcct
     1021 taccgtatcg tagaacgtcg cccaggtgat atcgctgcct gctactcaga cccagcaaaa
     1081 gctaaagcag aactcggttg ggaagcagaa ctcgacatca cccaaatgtg tgaagacgca
     1141 tggcgttggc aaagcaagca tccaaatgga tttgaagact aagatgatga tttcaatcat
     1201 cgtcccttgt ttaaacgaag aggaagtact tcctcttttt tatcaggctc tggaagcttt
     1261 acttccagat ttggaaacag aaatcgagta tgtctttgtc gatgatggat caagtgatgg
     1321 gaccttggaa ctcttaaagg cctatcggga gcaaaatccg gcagtccatt atatttcttt
     1381 ctctcgaaat tttggcaaag aagcagccct ttatgcaggc ttgcaatatg cgacaggaga
     1441 tttggtggtg gtgatggatg cagacctcca agatcctcct agtatgttgt ttgagatgaa
     1501 aaatgtacta gacaaaaatg tagacttgga ctgcgttggg acacggagaa ctagtcggga
     1561 gggagaaccc ttctttcgca gtttctgtgc tgttctcttt tatcgcctca tgcaaaaaat
     1621 cagcccagtg gctctgccgt cgggtgtccg tgattttcgt atgatgagaa ggtctgtagt
     1681 cgatgccatt ttaagcttga ccgagtccaa tcgtttttca aaggggctct tcgcctgggt
     1741 gggtttcaaa acctactatt tggactatcc aaatgtcgaa aggcaggctg gcaagaccag
     1801 ttggagtttt aggaaactct ttttctactc cattgaaggg attattaatt tttcagattt
     1861 tcccttgatt atagcctttg tagctggtct cctatcttgt tttctttctc tgctgatgac
     1921 cttttttgtt gtggttcgga ccctcatttt gggcaatccg acatctggtt ggacctctct
     1981 gatggctgtt attctctttc ttggaggcat tcaactcttg accattggga ttctcggcaa
     2041 gtatatcagt aagatttatt tagagactaa aaaaagacca ctttatctta tcaaagaaaa
     2101 aagtgatgat aggctaaaaa agatagaatt tatctgacat agtcactttt tgttatttgt
     2161 aattctttta gttcggagtt tgggtgattt ttaaaagaat tagggatttg aaatgattta
     2221 aaaaatctat gtagactaaa tttaaattgt agtgacttaa taaataaaaa atttaggagt
     2281 attatgttat gctaagtaaa gaggattata ctgaagaaat cgggctaata aagaaacaaa
     2341 attatgtaga ggctgaattg tatcctatag ttgctgatat aattaagcca actttaaaag
     2401 atagtttatc aaaaagatat gtcttcggaa gacaaagaag gggtttggga caaatttatt
     2461 atggtctaag taattttcct gatatagtta ttttagataa gacttatgaa aacaaatcta
     2521 ggaaaagtat aaaaattgaa gaatggaaga aacttcgagg atgcgtggaa gttaagaatt
     2581 taaattatag tttaattaca gaggaaaaaa tcaaatcaac catatcgaac agttttgaac
     2641 atataactgg agagatggaa caactaactg gagagatggg acaacttatt ggagaccttt
     2701 tatggtataa aaaagtaatt tatacaaatg gaattgaatg gagatttcta agtttagatg
     2761 acaaggaaga gatagataat acaattgttg aagtagttaa caaaagaata gaaacagaag
     2821 aagcgggaaa ttcttttgat tggtggaaga atattaaaga tttatcgttc aattatacgg
     2881 atatatgcct atctaaagat tgtagacaag agtggaatga atttgtaaaa agggtaaagg
     2941 aaatagaatg gtagaagtct attctcattg agaaatttag attttaagtt acagtaagat
     3001 ttatttagag actaaaaaaa gaccacttta tcttatcaaa gaaaaaagtg accttcctga
     3061 ttttacagaa aaaaataaag tgaaaagact ataattttac atggaaatgt gctaaactag
     3121 agggagtgga ttatcgccat tgatttaggc ttcctggttt ccaatcaggg cgcgagttgg
     3181 gcaattttta acagcctcta agacatcctg actaggagaa atttcttttt ccagttggtc
     3241 agggtcatcg taaaaacgca cgattccatt atcgtggtaa tcaaataaat cagaataagt
     3301 ttggcaaagc ccacaggcga tacatcgttc aggtataagt gtgattttca tgttcatatt
     3361 tatattgtaa taagaaaagt aaaaaaaaac aaggagtaag gtatggcaaa agaaccgtgg
     3421 caagaagata tctatgatca agaagaatca agagcagagc gtcggcatcg aaaccacgga
     3481 ggggctgata ggatggctaa tcgtattttg acgatcctag ctagtatttt ctttgtaatt
     3541 gtggtggtga tggtcatcgt tctcatctat ctatcatcgg ggggaagtaa tcgcacagca
     3601 gccttaaaag actttcatga ttctgatgca agtgtagtac aaatctcatc ttcaagcagt
     3661 tctcagcctg agcagagttc agagccagaa tctacttcta gtagttcaga agaagctgct
     3721 aatcctgaag gaacgattaa agttctcgca ggagaagggg aagcagctat tgccgctcgt
     3781 gcaggaatct ccattgctca gttagaggcc ttgaatcctg ggcacatggc tacaggatct
     3841 tggtttgcta atccaggtga tgttataaaa ataaaatagg agccgatatg aaaacaattc
     3901 aaattgctat tgatggtcct gcttccagcg gtaagagtac ggtcgcaaag attattgcta
     3961 aggattttgg attcacctac cttgatacag gagctatgta tcgtgcagcg acctatatgg
     4021 ctcttaagaa ccaattagga gttgaagaag tcgaagccct tctagccttg ttggaccagc
     4081 atccaatcag ctttggacgt tcagaaactg gagaccagct tgtttttgta ggagatgtgg
     4141 atattaccca tcctatccgt gaaaatgaag tgaccaatca tgtttctgct attgcagcaa
     4201 ttcctgaagt gcgtgagaaa ctggtttctc tccaacaaga aattgcccag caaggcggga
     4261 ttgtcatgga tggtcgcgat attggaactg ttgtattgcc acaagcagaa ttgaaaattt
     4321 tcctagtagc ttctgttgat gagagagcag agcgtcgtta caaggaaaat attgccaagg
     4381 gaattgaaac agactttgaa accctaaaaa aggaaattgc tgcgcgtgac tacaaggata
     4441 gtcatcgtga gacttctcct ctcaaacaag cagaggatgc tgtctacctt gatacaactg
     4501 gtttgaacat tcaagaagta gttgaaaaaa tcaaagcaga agctgaaaaa agaatgtaaa
     4561 tgtagaaaag ccgatagatt gatatcggtt tttttctaaa tttgtcaaaa tgctgatttt
     4621 agggtataat agttgctgtt cgagaaattt tgattttcaa agaatagtga atgatgataa
     4681 ggagaaacca tgaacaattt accaaattgt ccaaaatgta actcagagta tgtctacgaa
     4741 gacggtgccc tactggtttg cccagagtgt gctcatgagt ggaatcctgc tgaagttgca
     4801 gaagtagaag aaggtcttgt cgctatcgat gccaacggaa ataaattggc tgatggtgat
     4861 acagtaactc tcatcaagga cttgaaagta aaaggtgcgc caaaagattt gaaacaaggg
     4921 acgcgcgtga aaaatatccg catcgtagaa ggcgaccaca atatcgactg taagattgat
     4981 ggttttggtg ccatgaaact taaatcagag tttgtgagga agatttaagc atgtcaaaac
     5041 actataaact tgtattttat agccgtatct tcttgtttct agcggctttt acgggagttt
     5101 atcttgaaat tactaagcat ggtggttttg ggatgcttct ctattacacg gttctgtcca
     5161 acctcttggt aactattttt accctttatc ttctaaaggt tatgagccgt gtaggtgaaa
     5221 attggcaaag gccaagtctc ttgcgcttaa aaggtggggt caccatgagt atcatgatta
     5281 cctgtgtgat ttaccatttc ctcttggcgc ccattgcgac taatttctat accctagaaa
     5341 atttcctttg ccactatatc gttcccatct ggtttttagc ggataccctc ttttttgaca
     5401 aacagggtca atacaagatt tgggatccag cagtgtggac cattttaccc tttctgtata
     5461 tgatgtttgc tctttttaat ggcttggttc taaaactcaa tattccaaat gccaaggata
     5521 atcctttccc ttacttcttt ttgaatgtga acaagggttg gaatgttgtg tttaagtggt
     5581 gtctgattat ctttgttgcc tatatggtag caggatttat tttctacttt atcaagcaaa
     5641 tcaagagaaa gtcatcct
//
LOCUS       DAVBCW010000115         5586 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVBCW010000115 DAVBCW010000000
VERSION     DAVBCW010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.115"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(385..2274)
                     /locus_tag="VQ514_001814"
     CDS             complement(385..2274)
                     /locus_tag="VQ514_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HET2500543.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWSRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSRFERDCLNLAFSGKKEATLSNLFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGLEKTLQVGMG
                     VLTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDKAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            2412..3977
                     /locus_tag="VQ514_001815"
     CDS             2412..3977
                     /locus_tag="VQ514_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HET2500544.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIALPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            3977..4717
                     /locus_tag="VQ514_001816"
     CDS             3977..4717
                     /locus_tag="VQ514_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2500545.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEKIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(4826..5041)
                     /locus_tag="VQ514_001817"
     CDS             complement(4826..5041)
                     /locus_tag="VQ514_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500546.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
BASE COUNT         1750 a         1260 c          974 g         1602 t
ORIGIN      
        1 cggtgggcac tcatacaagc tgccaaagca tgcgctcgct tttcacctgc ttttaaggcc
       61 tatcttaaga ctaagttaga acaaggaaaa cattacaatg tagccatcat ccaccttgca
      121 aaaaaactta tccgaaccct gttttatatc ctaaaaaaga gctgccattt gacgaacaaa
      181 aagtgatgta atttctgaaa ttaacacagg attacctttg aaaaaatgga ctttttcaag
      241 ggctttttgc cgtgctctaa aatcatcgcc aaatcatcaa attgaaataa gaaatagttt
      301 agataaaact attgactttt cttatagttt gtctttcttc tatcttccat tatatcatac
      361 tttttcagtc tatggtagct ctctttaaaa ggctccgata ctgccgccac ctccgcctcc
      421 agagaagcca ccgccagaac ttccacttcc agaagatacg gagtaggtac ttgctgtatt
      481 tgcgacacta gcataatggc tcatttgcgc gcttgaatga taaaacatac tgtgccagcc
      541 ataagctaca tagagattga tatctggatt ttcaacttga atctgatgaa ccttcatcaa
      601 atgactaacc ttgtccgcat agccaaataa ggttgcatag accaagagac gattccaaac
      661 aacaatactt tccaattcag ccttatccag tcgtgcaatc tcacgtaaca tattttcaaa
      721 actggtccag agatagtaga cctcagctcc cgcttcattt agaacaccat cacgattatc
      781 tagtcgaagc ttccaataat agaaaacaga caaaactaac cctagaaaac caagtattgg
      841 caaagggagg taaagatagc catgaacgtc taaactgtac aagaacaaac caaatccgat
      901 aaataggggc aagatagtca agacacccat acccacttgc aaagtctttt ccaaaccagt
      961 taaaggacga tagtaatctg ggagccccca gaaggaaact cgttttctca ctccttcttg
     1021 catctggttc aatacctctt caaaagaaga tttgagttga agccctcttg cttgaatccg
     1081 tttttcatca gaaactttgg ctctacgata aagactatca gataccttgt aatccgcaaa
     1141 caaattggaa agagtggctt cttttttgcc tgaaaaggct agatttagac agtctctctc
     1201 aaatcttgac aaaccatctt ctttcaccaa tctcaagcca actgcatctc cttctgaaat
     1261 aatagagaca ttcccacggt ctatcacatc tagcaaggta gcttgaataa gttgatcaaa
     1321 ggtgaatttt ccagctccct tgaccaaggg actcacttcc tccaaggagg tcgagtagac
     1381 tgcttctgat aaaaccatag gctctaattc cattggtggt tcatagagac gatgattttt
     1441 ggcatatttg actgaaggag tggtctttct tctataaata aaatagaagc agacactcaa
     1501 taacaaggag atagaaagta tcgaagggag gacccaagta acgagttgtt tactctgatc
     1561 tttttctcta acaatcgagt cttctatctt attaaactct tctaaacgat tccctttcaa
     1621 gccctgatcc ctagcgctag caaaatcggt ccgagaccaa taggcatgca actcaactcc
     1681 acgcttagcc ggaagattgt ctaaacggat agtataatca aggttactct tttcaatcgt
     1741 tccctctcta aaaagtttcc ctgtatggaa aaagagtttt tcagccccct tgtctcccct
     1801 tacatgaaat tcaaactttt caatagactc tgaactatct gtcagaggtt gccaatttaa
     1861 ttcagcgata tcatcataaa ggaaaagtaa attttttaag ttccagacga ggtcaacttc
     1921 aactatgtcg ccctcctgac ctggattata gactctcaca gtataaccat ccgcttcttc
     1981 tgttacttcg ctagtcacat ctgctagttc tgcaccgttt ttcgcagcct gaacctttgg
     2041 atgagggtca atgtcaaacc cgctaggcat cttaccagca cgtccaagtc ccacgatttg
     2101 gcccttaaag tcctcctcaa actggtaaac tatcttctgt ctaaactctg ccgtattatc
     2161 tgcatgaata tacaaatccc cttgataaga gtttatcttg aaatcaatgg caaaaacaga
     2221 gagtggaaga aggcaaaaca agcctaacac cagtaagaaa aaagtttttt tcatcaacta
     2281 agcccccttt ttatctttct tatcattata gcatcttttc gtaagtaaag gcttacttat
     2341 gttgaaaaat tacgcatttt tcgataaaat aggagacagt tattttttaa tagatcagaa
     2401 taggagaaat catgagaaaa atatacttat ctattttcac aagtctcttg ctgatgctag
     2461 gacttgtcaa tgttgctcaa gccgatgaat atttacgcat cgggatggaa gcagcatatg
     2521 ctccctttaa ctggacccag gatgatgata gcaacggagc tgtcaaaatc gatgggacca
     2581 atcagtatgc caacggatac gatgttcaaa tcgccaagaa aatcgctaag gacttaggta
     2641 aagaaccttt ggttgttaaa accaagtggg aaggtctagt ccctgccctt acttctggta
     2701 agattgacat gattatcgca ggtatgagtc caactgcaga acgcaaacaa gaaattgcct
     2761 tttcgagcag ttactacact agcgaaccag ttttgcttgt caaaaaagat tctgcctacg
     2821 caagtgctaa atctttggat gactttaatg gtgcgaaaat cacttctcaa caaggtgtct
     2881 acctctacga cttgattgca caaatcccag gtgctaaaaa agaaacagcc atgggagact
     2941 tcgctcaaat gcgccaagct cttgaggctg gtgtcattga tgcttatgtt tctgaacgtc
     3001 cagaagcact gactgctgaa gctgcgaact ctaagttcaa gatgattcaa gtagaacctg
     3061 gtttcaaaac tggggaagaa gatacagcta tcgctatcgg gcttcgtaaa aatgacaatc
     3121 gtattagcca aatcaatgcc agcattgaaa ccatttcaaa agatgaccaa gttgccttga
     3181 tggatcgtat gatcaaggaa caacctgccg aagctacaac aactgaagag actagcagta
     3241 gtttctttag ccaagtcgct aaaattcttt ctgaaaactg gcaacaactc ttgcgtggtg
     3301 ctggtatcac tcttttaatc tctatcgtcg gaaccatcat aggtctcatt attggacttg
     3361 ccattggtgt cttccgtact gctcctctct ctgaaaacaa agccatttat ggccttcaaa
     3421 aactagtcgg ctgggttctc aatgtctaca ttgaaatttt ccgtggtacg ccaatgattg
     3481 ttcaatcgat ggttatctac tatggaactg cccaagcttt cgggatcaac cttgaccgta
     3541 cactggctgc tatcttcatc gtttcaatca ataccggtgc ctacatgact gaaatcgtcc
     3601 gtggtggtat cctagcagtt gacaagggac aatttgaagc tgcgactgct cttggtatga
     3661 cccataacca gaccatgcgt aagattgccc tacctcaggt agtccgtaac atcctacctg
     3721 caactggtaa tgaatttgtc atcaatatca aagatacatc tgtattgaac gttatctctg
     3781 ttgtcgaact ttatttctca ggaaataccg tggcaacaca aacctatcaa tacttccaga
     3841 catttacaat catcgccgtg atttactttg tcctcacctt caccgtaaca cgtatcctac
     3901 gatttatcga gcgccgaatg gacatggata cctatactac aggtgctaac caaatgcaaa
     3961 cggaggattt gaaataatga cacaagcaat ccttgaaatt aaacacctca aaaaatccta
     4021 tggacaaaac gaagtgctaa aagacatttc actcactgtc cacaagggag aggtcatctc
     4081 tatcatcgga agctctggaa gcggaaaatc gaccttccta cgctccatta acctacttga
     4141 aacaccaact gatggacaaa tcctttatca tggacaaaac gtcctcgaaa aaggctatga
     4201 cctcacgcaa taccgtgaaa agttggggat ggttttccaa tcctttaacc tctttgaaaa
     4261 tctcaatgtt cttgaaaaca caatcgtcgc tcagacaact gtcctaaaac gcgaacgcac
     4321 agaagctgaa aagattgcca aagaaaacct ggaaaaggtc ggcatgggag aacgctactg
     4381 gcaagccaaa ccaaaacaac tctcaggtgg tcaaaaacaa cgtgtggcca tcgctcgtgc
     4441 cctctccatg aatccggacg ctattctctt tgatgaacca acatcagctc tcgatccaga
     4501 aatggttgga gaagtcctca aaatcatgca ggacctggct caggaaggct tgaccatgat
     4561 tgtcgtaacc catgaaatgg aatttgcccg tgatgtctct caccgtgtta tctttatgga
     4621 taagggcgtg atcgctgaag aaggtaaacc agaagacctc ttcaccaatc ctaaagaaga
     4681 ccgaacaaaa gagttccttc aacgctatct caaataaaaa gaaaaggctg catcaaccgt
     4741 gcagtctttt tgctgtcctc aaaatgaaaa ggcagattct ctatctctac tacttgaagg
     4801 tcaagaatcc gccgttatcc aaagattaat cttcaaattt ttcataattt ttttcataga
     4861 aatcaattaa gcctagggct gtttcaaatg aaatattttt tacttttgca cgaccctgcg
     4921 ccagagcaat gatagacatt tcacgcgcat tcgtttcttt agagatacgg tagcctgtga
     4981 ttttcttatc acgaacccag ccaacaactg attctacttt ttcaaagttt gacttagcca
     5041 tgtttttctc ctattttctt ctttataata tttaattgta tacgttttta ctacttttgt
     5101 caataaaaaa aacatatatt caacgaaaat aattagttca gaattttctt acttcctttt
     5161 taaatccgat aatatatagg ttttgctagc ctctacacca taaatatttg ctaataaaac
     5221 tactgtatta ttaccctctt aaggtgcgaa attattcaag ttcttaaaaa atgcttctct
     5281 atttccgtaa gatagcttat ttcttcgatt taagaactga agatgatagt tctaaccttt
     5341 agaaatccaa gccttcacta gctcaaaaac tcctatcttt cttcctcaat aaatcatctg
     5401 agagataggt attccttata tccttcaaat tcatcactcc tttggatatt gaggtgctag
     5461 cctagaagca gacacccgat tatctaaacc atccttaata gagatgacaa gacagataag
     5521 ctcagttaat tacttatata tctttttaat atggtatatt tgtcaacaaa aagaaatcaa
     5581 atcata
//
LOCUS       DAVBCW010000116         5507 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVBCW010000116 DAVBCW010000000
VERSION     DAVBCW010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5507
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.116"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(2..196)
                     /locus_tag="VQ514_001818"
     CDS             complement(2..196)
                     /locus_tag="VQ514_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2500547.1"
                     /translation="MKGKEYENRTVSFAISSKGEKMKVENISYRVDHRILFDNISFDT
                     SSSDVTLITGKNGTGKSTLL"
     gene            complement(656..1384)
                     /locus_tag="VQ514_001819"
     CDS             complement(656..1384)
                     /locus_tag="VQ514_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET2500548.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            1542..2951
                     /locus_tag="VQ514_001820"
     CDS             1542..2951
                     /locus_tag="VQ514_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HET2500549.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLGLDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTEDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKAIYDIAMIVKEEYGNIPWFISENGMGVENEARFIDENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSLCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            2969..4264
                     /locus_tag="VQ514_001821"
     CDS             2969..4264
                     /locus_tag="VQ514_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2500550.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTKFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPSAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGIVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            4269..4577
                     /locus_tag="VQ514_001822"
     CDS             4269..4577
                     /locus_tag="VQ514_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2500551.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            4574..4882
                     /locus_tag="VQ514_001823"
     CDS             4574..4882
                     /locus_tag="VQ514_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2500552.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
BASE COUNT         1864 a          926 c          976 g         1741 t
ORIGIN      
        1 actatagtaa agttgacttt cctgtaccat ttttaccagt aattaatgtc acgtctgaac
       61 tcgaagtatc aaaagaaata ttatcaaaca atatacgatg atccacccta tacgaaatat
      121 tttctacttt cattttttct ccttttgaac taatagcaaa agaaacagtt ctgttttcat
      181 actctttccc cttcatctat attattcgta atgaaaaata aaaaaataaa atttttttaa
      241 atgattatga tccagaattt atttaatata ttaaaataat atataagcaa ataaattggg
      301 agattgaaat tcatttctag caatttgtta gaagtcacag tctactattc taacctcagt
      361 atactatatg tgttacaaga aaagactcaa aaacttctcg tcagtgtaag aatgtttgaa
      421 acactattta aaactaaata actataagaa gtagatcagg tgaacaattt cagtacctac
      481 tctataagct atacgaagat atttttcaac atgatggata aaacgatcac tcttttaaag
      541 ccatcttatt tagaaaagag aaaccattta agcatagatg ggaccttctt gccctgtttt
      601 aaatttttct taaactagag cctgtaaaag attggcttca ttttcacatt ttctcctagg
      661 aggaatgtcg taatgcaaaa gaattaaatt caaataaatc atagcgatga cgactaatcg
      721 agtactcaaa cggagttccg ttatccaaat aagccacttg ttctacttca aataccggtt
      781 ctgttggaag gagctgcaag taatgttgct cattttctga actagaagaa gctcgcaaga
      841 tttttgtagc actggcaata tgcaatccta actttccttc aatgtaattg taaatcgatt
      901 tttgtaaaat atcttcagta atacctggaa taacatcggt actcatataa gtttgttcca
      961 gaacataagg tttaccgtca ataatacgca ggcggtaaat attataaact ggactctgca
     1021 aagcgacctg tagtttttct gctaaaaatt catttgcaaa ttctaatttg aagtgaataa
     1081 tcctcgagtc aattgtagaa tgagcatctt cagatatttt tgtcagtccc cggatatctc
     1141 tttctggaac gattaatttt ctttttgagc tgccacgaga gagaacaaag gttccctgcc
     1201 cacgttttct aaaaattaag ccctcagaaa ctaacaagtc taaagctttt ttgatcgtca
     1261 ttcgactaca ggcaaactgt ttagctaatg tcatttcatc aggcaattgt tcgttcatgg
     1321 tatatttatt acagagaatc ttttcacgaa tctcattgta aataaataaa tattttgtac
     1381 tcatttaatc tggttctctc tataatattc taatagtagt ataacaaagt tcaagaaatg
     1441 aggcaaacac aatatatgta tcaacaattt ttaaaaatgt ctagacaaat tttaaaatta
     1501 tgctatactt tatgtatagc ataatagaaa ggaggttgag catgaaaaaa aagttagtat
     1561 ttcctaatct gttttggtgg ggagctgctt ctagcggacc tcagacagaa ggtcaatatg
     1621 gaaaagtaca tgaaaatgtg atggactact ggttcaaaac gcatccagaa gattttttcg
     1681 ataatgtcgg acctcttgta gccagtaact tttttcatac ttacaccgaa gatttccact
     1741 tgatgaagga aattggagtt aattctttcc gcacttccat ccaatggagt cgactcatca
     1801 agaatttaga gacaggtgag cctgatccaa aaggtattgc tttctacaat gccatcattg
     1861 aagaagctaa aaagaaccag atggatcttg tgatgaattt acatcatttt gatttaccag
     1921 tggaacttct tcaaaaatac ggtggttggg aaagcaaaca tgtagtggag ttattcgtga
     1981 agtttgccaa gactgctttc acatgctttg gagataaggt tcattactgg acaactttca
     2041 atgagccaat ggtcattcca gaagcaggat acttatatgc tttccattat ccaaatctaa
     2101 aaggaaaggg aaaagaggcc gtacaagtca tctataatct aaaccttgct agtgcaaaag
     2161 tgattcaact atatcgctca ttaggacttg atggaaagat tgggattatt ttaaacttga
     2221 cacctgctta tccaagaagt aattctccag aagacttaga agcaagtcga tttacagaag
     2281 acttctttaa caaagtcttc ttgaatccag ctgttaaagg aactttccca gaaagattgg
     2341 taaaacagct agagagagat ggcgtgttat ggagtcatac cgaaaaagag cttcaactga
     2401 tgaaatcaaa tacggttgat tttcttggag taaactacta ccatccaaaa cgtgttcaag
     2461 cacaagcaaa tcctgaggaa tatcagacgc cctggatgcc agaccaatac ttcaaagagt
     2521 atgaatggcc ggagcgtcgc atgaatccat atcgtggttg ggaaattttt ccgaaagcca
     2581 tttatgatat tgctatgatt gtgaaggaag aatatggtaa tatcccatgg tttatcagtg
     2641 agaacggaat gggtgttgaa aacgaagcac ggtttatcga tgaaaatgga gttatcgatg
     2701 acgtgtatcg tattgaattt tatgaagaac atttaagatg gctacataaa gccattgaag
     2761 agggaagtct ctgttttgga taccacgctt ggaccgcatt tgattgctgg tcttggaata
     2821 atgcatataa gaatcgttac ggatttatct ccgttgattt agaaacgcaa aagagaacca
     2881 tcaagagctc aggaagatgg tatcgcaaag taagtgacaa taacggtttt gaagtagaaa
     2941 ttgaggagta aagagaggag atattcagat gaaaaactca aaatttatag atcaatttgc
     3001 cacctttgct ggtaaactag ggaaccaaat tcatttaaaa accctaagag atgcattcgt
     3061 aacagtaatg ccattatata ttttggcagg tttaatcgtt cttttgaaca acacggtatt
     3121 taagtggatt ttccaagggg atacattaac aaaattccaa tattggggaa taacaattgc
     3181 aaacggtact ttaagtattt caggtatgat tattgctgta atggttggtt atttcttagc
     3241 taaaaacaga gatttcgaaa acccgtcagc agcatcaatg ctatcattag tttctttaat
     3301 tgtgatgatg ccaaatacag tttctgtagt tcctgacgga gcaaaagatg ctgtaaacat
     3361 ttcaggtgtt ctttcattca acaacacagg tacaggcgca atgttcgccg gggttatcgt
     3421 agcgattatt gcaacagaat tattcattga attatcaaac gttaaagctt tacaaatgaa
     3481 ccttggtgaa aatattccac cagctgttag tagatcattt agcgtattac ttccagtcat
     3541 gactgtcatc tccttatttg ggattgtttc agcattatta ttcaatataa ctggaatgaa
     3601 cttaatctca atcattacaa tctttattca agaaccaatt cgtcatattg gtacaagctt
     3661 aatcggggtc attattattt actctttagg aaatatgtta tggctatttg gtattcacca
     3721 agcagttatt tacagtgcca tcctagaacc attactatta attaacatta ctgaaaacat
     3781 cactgcagca aataatggac aagccattcc acacatcatc aacttatcac aaatacaaac
     3841 attcgcttta atgggtggta gtggatctac attatgttta ttaatagcaa cattcttagt
     3901 gagtcgcaat gctgtttcta aaaacgtggc taaattatct tttggacctg gtatcttcaa
     3961 tatcaatgaa ccagtattat tcggttaccc aatcgtttat aacatttcat tagctattcc
     4021 atttatcaca gttccagtcc ttggtatttt aatcagctac ttagcaacag ttacaggatt
     4081 catgagtcct gcatttatac aagttccttg gactacacca gtattcttaa atgcatggtt
     4141 agcaacagca ggggacgtga gagcagttct agttcaattc atcatctttg cacttggagt
     4201 tcttctatac attccattta tcaaagttaa tgacaaagtt gttgagcaag aaatggaagg
     4261 ttaagattat gaaacaaatt ttattagtat gtaatgcggg aatgtcgaca agtatgcttg
     4321 ttaaaaaaat gcaacaaagt gcgactgaac gtgggattga aatttctatc caagcaaaat
     4381 caatgacaga agcgaaaaag aatatccatg aagcagatgt cattctaatt gggccacaaa
     4441 ttcgttatga gttacttgct gtgaaagaaa ttgcaggaaa tatccctgta gataccattg
     4501 atatgcggga ttacggaatg atgaatggag caaaagttct ggaacaagca cttgcatgga
     4561 taggagaaat aagatgacag aagaaatgga atatatctgt tttcagctca ttgccaatag
     4621 tggagcagcc aaatcatcct ttattgaagc catccaacta gctaaggcag gaaatttaaa
     4681 agaagcgaaa attaaagttg aagaagcgga agactcctta gtagaagcgc acaagattca
     4741 ctctaatctc atacaaaaag aagctactgg agaaaaaata ggattctcct tgctatttat
     4801 gcatgcagaa gaccaaatgg cttcaacaga gattattcag ttgctatcta aagaattcat
     4861 cgatctctat caaaataaat aggaacactg tcgttcccat ctgtctttat taatgacggc
     4921 tacattgtag gaacctccaa aagttggaca aaatgattga aaggattaga tcctgcatta
     4981 cacaagatcc aatccttttc ttttactcta gtttttataa tgtgttgaat ttagaatagt
     5041 ccactgcagt ttctaaaaaa ttgctggaaa tagatgttaa gctccaattt atttgtttat
     5101 atcttatttc agtttactat actagcttat tattctagta aaaaattgaa tgagccacta
     5161 tttatttaat atgtatgatg agggaatgac tcaaaaactt cttgtcgata taagaacatc
     5221 tgagatagta ttttaaaacg aaaggactat aaaagctcat ttccttcgca cttattttat
     5281 agagatctat cattatactc tttagaaatc aaaaagcaac acaccttgtc atcgttttat
     5341 cgtaaacctg ttaatgaggg gctagtctta tctgcaatct caaatcagtg ttttgagctg
     5401 aatggcactc gttttctagt ttgctctttg attttaattg ggccttagta aatactactc
     5461 ttaaattaaa ctatagtgaa ttgaaataag atgtgaacaa ctctatc
//
LOCUS       DAVBCW010000117         4985 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVBCW010000117 DAVBCW010000000
VERSION     DAVBCW010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4985
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.117"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(44..283)
                     /locus_tag="VQ514_001824"
     CDS             complement(44..283)
                     /locus_tag="VQ514_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HET2500553.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            complement(303..575)
                     /gene="rpsP"
                     /locus_tag="VQ514_001825"
     CDS             complement(303..575)
                     /gene="rpsP"
                     /locus_tag="VQ514_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HET2500554.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            745..954
                     /locus_tag="VQ514_001826"
     CDS             745..954
                     /locus_tag="VQ514_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500555.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERELDTWLLSPKPVPKRNMDL
                     LVDDSLAGDIILLWRIQFGTFTTET"
     gene            1433..1654
                     /locus_tag="VQ514_001827"
     CDS             1433..1654
                     /locus_tag="VQ514_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500556.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEEVEDFQDDDEIPTII"
     gene            1737..2540
                     /locus_tag="VQ514_001828"
     CDS             1737..2540
                     /locus_tag="VQ514_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET2500557.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQSSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(2596..4137)
                     /locus_tag="VQ514_001829"
     CDS             complement(2596..4137)
                     /locus_tag="VQ514_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET2500558.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMEMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DLPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(4139..4621)
                     /locus_tag="VQ514_001830"
     CDS             complement(4139..4621)
                     /locus_tag="VQ514_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET2500559.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVYFCFTPQEQSTVGVGTPDIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN
                     "
     gene            complement(4621..>4985)
                     /locus_tag="VQ514_001831"
     CDS             complement(4621..>4985)
                     /locus_tag="VQ514_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S rRNA methyltransferase"
                     /protein_id="HET2500560.1"
                     /translation="LFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALELAELLKNIK
                     KSSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGGNCVVRQEHGTDIDAACGQL
                     LFNTMKRDRQKAVAAVNP"
BASE COUNT         1529 a         1058 c          935 g         1463 t
ORIGIN      
        1 cttctattcc acacaaaaaa gcgagacatc cgtcccgccc ttcttatttt tcgtcaataa
       61 cgattcttac ttttttgtat tcagttggga cagagtagac aatcgttctt atcgcagaaa
      121 tagtgcgacc cttacgaccg attacacgac ccacatcgct ttgatcaaga ttcaaatgat
      181 attccaaaaa ttctggtgta tcctcaatct tgatagttaa ggcatctggt tgtgaaatta
      241 agggtttcac aatcgcaata atgagatttt caatcgtatc catctgtcaa cctactttaa
      301 acttattttg aaaatttaga atcgtggaat tttttcaata cgccttcttt tgaaaggatg
      361 ttacgtactg tgtctgaagg ttgagctcca ttagccaacc atgcaagaac gcggtcttct
      421 ttcaaagtta cttggttttc agcaacaagt gggttgtaag ttccaactgt ttcgatgaaa
      481 cgtccgtcac gtggtgaacg tgaatctgct acgttgatac ggtagaaagg ttttttctta
      541 gaacccatac gagtcaaacg gattttaact gccattttta aagtctcatt tctttaattt
      601 tttatttcgg tgaaatagct gagctattta gcacatgttc tattatagca gatttctggc
      661 atgtgtcaag aaaaaaactt gacagactat aaatttctta aagtatttag aaatttgtta
      721 gaataggaaa taaatgtttg aaagatgcta tccgtgaata ctattttaga aaaattttat
      781 aaagaacacc aagtcaaacc cttcatatcg cctgaacgag agttggatac ttggcttcta
      841 agtcccaagc ccgttcccaa gcgaaatatg gacttattag tagatgattc actagcagga
      901 gatatcattt tactatggag aatccagttt ggtactttta ccactgaaac atgattttaa
      961 aaactcccat tcgaatatga ttcaaaattc gcttagaatc aatgttttcc cgctttttca
     1021 aaaaataaat tttcatctaa tttaaataac tttcaattaa gtgtgaattt tgcttaaaat
     1081 cattaaaaag caccccatgg tgtgaaccat gaagtgttgt aaatgctgtg ttgtagctag
     1141 ttcttagcgt ttagagaact ggctagcttt acgagctttc ttaagatctg gtttccaaaa
     1201 tactttgagt acacttatgg tattgatgcc cctaaacatc taaaaactct ggtagaaaaa
     1261 gggtatgctc ttgtcgaaac ttcttttgac tcacttgacc atctaaataa taccatgaaa
     1321 aagaatatcc tgaagaagaa gggagttact ggactttcta aaatgaaatc ttgcgacctg
     1381 gatcaagccc ttcatgagca tttttcagaa gaagaattag ctggtcactt tcatgtcctt
     1441 ctatggactt ttttacaatg gcattgctat cacacccaat acctatctaa gcgcctggtt
     1501 cgtaaacttt attgcagctc ttcctctaaa tttcctaatt gttgaaccaa ttgcccgttt
     1561 tatactaagt tcttttcaga aaccatttac tggggaagaa gttgaagaag ttgaagattt
     1621 tcaagatgat gatgaaatcc caactattat ctaagccagt tctgtaaact actaatattt
     1681 gaaatccact tctttttagg gtacaatggt ataaatgaat ttttgagagg atcagaatga
     1741 aaaaactagc aacccttctt ttactgtcta ctgtagccct agctgggtgt agcagcgtcc
     1801 aacgcagtct gcgtggtgat gattatgttg attccagtct tgctgctgaa gaaagttcca
     1861 aagtagctgc ccaatctgcc aaggagttaa acgatgcttt aacaaacgaa aacgccaatt
     1921 tcccacaact atctaaggaa gttgctgaag atgaagccga agtgattctc cacacaagcc
     1981 aaggtgatat tcgcattaaa ctcttcccta aactcgctcc tctagcggtt gaaaatttcc
     2041 tcactcacgc caaagaaggc tactataacg gtattacctt ccaccgtgtc atcgatggct
     2101 ttatggtcca aactggagat ccaaaagggg acggtacagg tggtcagtcc atctggcatg
     2161 acaaggataa gactaaagac aaaggaactg gtttcaagaa cgagattact ccttatttgt
     2221 ataacatccg tggtgctctt gctatggcta atactggtca accaaacacc aatggcagcc
     2281 agttcttcat caaccaaagc tctacagata cctcttctaa actccctaca agcaagtatc
     2341 cacagaaaat tatcgaagcc tacaaagaag gtggaaaccc tagtctagat ggcaaacacc
     2401 cagtctttgg tcaagtgatt gacggtatgg atgttgtgga taagattgct aaggccgaaa
     2461 aagatgaaaa agacaagcca actactgcta tcacaatcga cagcatcgaa gtggtgaaag
     2521 actacgattt taaatcttaa aaaccaaaaa aatacagtat ccacattcgg tactgtattt
     2581 cttttactct cattcttaag ttaaattatt aaaatcccat atttggtcta tccagccttc
     2641 ataaaagtct ggctcgtggc agaccataag gatagatccc ctatattctt tgagagcgcg
     2701 tttgagctca tcctttgcat ccacatccaa atggttggtc ggctcgtcca gcactaaaac
     2761 gttgttttca cgattcatca agagacagaa acgaaccttg gcttgctctc cccctgataa
     2821 tacttgaatc tggctttcaa tatgtttggt tgtcaaacca caacgggcaa gggctgcacg
     2881 gacttctgct tgattaaggg caggaaaggc attccagaca gcttcaagag gagtttggcg
     2941 attaccgcct tctacttcct gctcaaaata accaagttct aaataatctc cacgctccac
     3001 ttccccagcg attggcgaga taatgcccaa gagactcttc aagagagttg tttttccaat
     3061 accattagca ccaataatcg caaccttttg attgcgttcg aaggtaagat ttaaaggctt
     3121 agtaagagga aggtcgtaac caatttgcaa gttcttggct tggaagataa agcgccctgg
     3181 tgtacgagct ggtttgaaat caaaggatgg ttttggtttc tcactttgga gttcgataat
     3241 atccatctta tccaatttct tttgacgaga catagccata ttacgagttg caacacgggc
     3301 tttattacga gccacaaagt ccttgaggtc tgcaatctct ttctgctggc gttcgtaggc
     3361 tgcctctagc tgagatttct tcatagcata aacttcttgg aactggtagt agtcaccaga
     3421 gtaacgcgtc agctgttgat tttccacatg atagacaata ttgataacgt cattgaggaa
     3481 tggaatatcg tgcgaaatga gaacaaaggc attctcatag ttttggagat agcgcttgag
     3541 ccaatcaata tgctcagcat ccaagtagtt ggtcggctcg tccaacagca agatatcagg
     3601 cttttcaagg agaagttttg ccaaaagcac cttggttctt tgcccacctg acaaagaagt
     3661 tacatccgta tccatgccaa agtccataac accaagagca cgcgctactt cgtcaatctt
     3721 agcatccaag gtatagaaat cacgactctc cagacggtct tgaagttctc ctacttcttc
     3781 catgagagca tcaacatccg caccgtcttc agccatttcc atatagaggt cattgatacg
     3841 agcttcagct ttgaaaagct catcaaaagc cgtacggaga acatcacgca ccgactgtct
     3901 ttcagcaagg acagagtgct gatccaagta accagccgtc acatatttgg accactcaac
     3961 ctttccttca tctggcagca ttttaccagt cacgatactc ataaaggttg attttccttc
     4021 accattggca ccgaccaggc cgatatgttc tcccttgagg agacggaagg acacatcttc
     4081 aaaaattgca cggtcaccaa aaccgtgact cagattttta acttctaaaa tactcatttt
     4141 aattccttat cttgttttta tgtaatcgtt tataaaggag ccaagccaga tagccaccca
     4201 aagtgttggt ccacaaatca tcaatctcaa agacgcgatt gaaatcaaag aaaaagtcca
     4261 agattaattg cgtacactcg attccaagac tcacaagaaa actaaaaaga aggacctttt
     4321 ttgttttccg caaatttgga aatagataaa ggagttggaa aatcagagga aaaaacaaga
     4381 agacattgag gatattttgt aaaaaaatcc aacataattg tccaatgtca ctcacttcgc
     4441 ccagtttcca gagagaattg aaaggagtca aaagaaaaac caggcgtcca agatgctgaa
     4501 tatctggagt tcccactccc acggtagatt gttcttgagg agtaaagcaa aaatagacaa
     4561 tgcaaatgct atagaaaatg actccccaga ccaaaatatg attataagtc ttcttcatca
     4621 ttaaggattt accgctgcga ctgccttctg gcggtcacgt ttcattgtgt taaagagcaa
     4681 ttgtccacaa gctgcgtcaa tatctgtacc atgctcttga cgaaccacac agttgccccc
     4741 ttttttctta agcgtatcat agaaagccaa cacgcgctct ttgggactac ggctatattg
     4801 gtcatgctca ctaactgggt tataaggaat caagtttaca taagacgatt tcttgatgtt
     4861 cttgagcaat tcagccaatt ccaaggcttg ttccacaccg tcgttgactt cattaagcat
     4921 gatatattca aaggttacac gacggtttgt tgtctcaatg tagtattcaa tagcagcaaa
     4981 gagtt
//
LOCUS       DAVBCW010000118         4890 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVBCW010000118 DAVBCW010000000
VERSION     DAVBCW010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4890
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.118"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            21..866
                     /locus_tag="VQ514_001832"
     CDS             21..866
                     /locus_tag="VQ514_001832"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HET2500561.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            complement(1013..2074)
                     /gene="dinB"
                     /locus_tag="VQ514_001833"
     CDS             complement(1013..2074)
                     /gene="dinB"
                     /locus_tag="VQ514_001833"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HET2500562.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIRDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKSVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGVSYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILLAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSKKERGVRLLGITMTGF"
     gene            2422..4746
                     /gene="pflB"
                     /locus_tag="VQ514_001834"
     CDS             2422..4746
                     /gene="pflB"
                     /locus_tag="VQ514_001834"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HET2500563.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKSNFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
BASE COUNT         1421 a         1003 c          974 g         1492 t
ORIGIN      
        1 tataatatag ggagtaatct atgtatctta ttgaaatttt aaaatctatc ttcttcggga
       61 ttgttgaagg aattacggaa tggttgccga tttccagtac aggtcacttg attttagcag
      121 aggaattcat ccaataccaa aatcaaaatg aagcctttat gtccatgttt aatgtcgtga
      181 ttcagcttgg tgctatttta gcagttatgg tgatttattt taacaagctc aatcctttta
      241 aaccgactaa ggacaaacag gaagttcgta agacttggag actatggttg aaggtcttga
      301 ttgctacttt acctttactt ggtgtcttta aatttgatga ttggtttgat acccacttcc
      361 ataacatggt ttcagttgct ctcatgttga ttatctacgg ggttgccttc atctatttgg
      421 aaaagcgcaa taaagcgcgt gctatcgagc caagtgtaac agagttggac aagcttcctt
      481 atacgaccgc tttctatatc ggactcttcc aagttcttgc tcttttacca gggactagcc
      541 gttcaggtgc aacgattgtc ggtggtttgt taaatggaac cagtcgttca gttgtgacag
      601 aatttacctt ctatcttggg attcctgtta tgtttggagc tagtgcctta aagattttca
      661 aatttgtgaa agccggagaa ctcttgagct ttgggcaatt gtttttgctc ttggtcgcga
      721 tgggagtagc ttttgcggtc agcatggtgg ctattcgctt cttgaccagc tatgtgaaaa
      781 aacacgactt cacccttttt ggtaaatacc gtatcgtgct tggtagtgtt ttgctacttt
      841 acagttttgt ccgtttattt gtataagaaa aaccttgaag gggcaactct tcaaggtttt
      901 atactcttcg aaaatctctt caaaccgcgt cagctttatc tgcaacctca aaacagtgtt
      961 ttgagcagcc tgcggctagc ctcctagttt gctctttgat tttcattgag ctttaaaatc
     1021 cagtcatggt aatccccaat aggcggacac ctctttcttt cttgcttaat tcttcataga
     1081 gttgcagggc tatttggctt atctgactag catcttgtgt tttttgagca agactttttc
     1141 gtttggtaag agttgaaaag tcctcgtagc ggattttcaa aatgacaatt tttccagctt
     1201 tttcttgttg atgtagattg agagcgactt tttctgatag aagagtcagc tcttttttga
     1261 tatcttcctc agcaaggaga atcttcccgt aggttttctc cttgccgatt gatttacgga
     1321 tgcgattgga tttgactgga gagttgtgaa tgccacgagc ctttcgatac agatcatagc
     1381 ctagtctacc aaaacggtct attagggtta cctcaggaac ttcaagtaaa tcagcaccag
     1441 taaaaacgcc catttgatga agacgttcta ctgtcttttt tcctactcca tgaaatttgg
     1501 aaatatccat ttgtttgaga aaatcctcag cctgttcagg tagaatcact gtcaaaccat
     1561 gtggtttttg ataatcactc gccattttag ctaagaattt gttgtaagaa acgcctgcgg
     1621 aagcagttag atggagttct tgccagatat ctttttgaat gaggcgagca attttgaccg
     1681 ctgacttgat accgagttta ttttctgtca catccaaata ggcttcgtca atgctcatgg
     1741 gttcaatcaa atctgtatag cgcttaaaaa tagctcgaat ctggagtccc acagacttgt
     1801 atttctcata attccctgag ataaagacag cctggggaca acgttcataa gcttccttgg
     1861 aactcatggc agaatggaca ccaaaagctc ttgcctcata actacaggta gaaacgactc
     1921 cccgtccacc tgtttgccga gggtcgcttc caataatgac aggttttcct ctgagtttag
     1981 gattatccct gatttccact gcagcaaaaa aggcatccat gtcaatatgg atgatttttc
     2041 ttgacaaatc atttaacaaa ggaaaaatca acatgcctag caccttttta tactcttcga
     2101 aaatctcttc aaaccccgtc agctctatct gcaacctcaa aacagtgttt tgagcaatct
     2161 gcggctagct tcctagtttg cttttcgatt tccattgagt gttactgctt attttctttt
     2221 attataccct tttttctgaa aaaaagaaaa aaggacttta ttttttcaaa aatataatac
     2281 agtttgaaat aaaatataga ctgttttaga aaagaaagtg taaaaatagg gaattttcac
     2341 ttgttgaaat cggttactat atggtatact tgtcttatga atgtaacaga tgactgttac
     2401 tagaaaaaag aggacattaa tatggttgtt aagacagttg ttgaagcaca agatattttt
     2461 gacaaagctt gggaaggctt caaaggcgta gattggaaag aaaaagcaag tgtatcacga
     2521 tttgtacaag ctaactacac accttatgat ggagacgaaa gcttccttgc aggaccaaca
     2581 gagcgttcac ttcacatcaa gaaaattgta gaagaaacta aagcacacta cgaagaaact
     2641 cgtttcccaa tggacactcg tccaacatct atcgctgata tccctgctgg atttatcgac
     2701 aaagaaaatg aagttatctt cggtatccaa aacgatgaac tcttcaaatt gaacttcatg
     2761 ccaaaaggtg gtatccgtat ggctgaaact actttgaaag aaaatggata cgaaccagac
     2821 ccagctgttc acgaaatctt cactaaatat gtaacaacag ttaacgacgg tattttccgt
     2881 gcctacactt caaatattcg tcgcgctcgt cacgcacaca ctgtaactgg tcttccagat
     2941 gcatactcac gcggacgtat catcggtgtt tacgcacgtc ttgctcttta cggtgcagac
     3001 tacttgatgc aagaaaaagt aaatgactgg aatgcaatca aagaaatcga tgaagaaaca
     3061 atccgtcttc gtgaagaagt aaaccttcaa taccaagcat tgcaacaagt tgttcgcctg
     3121 ggtgaccttt acggggttga tgttcgcaaa ccagcgatga acgtgaaaga agcaatccaa
     3181 tgggttaaca ttgctttcat ggctgtctgc cgtgtgatta acggtgctgc tacatctcta
     3241 ggtcgtgtac caatcgtatt ggacatcttt gcagaacgtg accttgctcg tggtacattt
     3301 actgaatcag aaatccaaga attcgttgat gatttcgtta tgaaacttcg tacagttaaa
     3361 tttgctcgta caaaagctta tgaccaattg tactcaggtg acccaacctt tatcacaact
     3421 tctatggctg gtatgggtaa cgacggtcgt caccgtgtta ctaagatgga ctaccgtttc
     3481 ttgaacactc ttgacaacat cggtaactca ccagaaccaa acttgacagt tctttggact
     3541 gacaaattgc catacaactt ccgtcgctac tgtatgcaca tgagccacaa acactcttct
     3601 atccaatacg aaggtgtaac aacaatggct aaagacggat atggtgaaat gagctgtatc
     3661 tcatgctgtg tgtctccact tgatccagaa aatgaagaac aacgccacaa catccagtac
     3721 ttcggtgctc gtgtaaacgt tcttaaagcc cttcttactg gtttgaatgg tggttacgac
     3781 gatgttcaca aagactacaa agtatttgat atcgaaccaa tccgtgacga agttcttgaa
     3841 tttgaatcag ttaaatcgaa ctttgaaaaa tctcttgact ggttgactga cacttacgta
     3901 gatgccttga acatcatcca ctacatgact gataggtaca actacgaagc tgttcaaatg
     3961 gccttcttgc caactaaaca acgtgccaac atgggattcg gtatctgtgg atttgctaac
     4021 actgttgata cattgtcagc tatcaaatac gctacagtta aaccaatccg tgacgaagat
     4081 ggctacatct acgattacga aacaatcggt gactacccac gctggggtga agatgaccca
     4141 cgttcaaacg aattggcaga atggttgatc gaagcttaca caactcgtct acgtagccac
     4201 aaactataca aagacgcaga agctacagta tcacttttga caatcacatc taacgttgct
     4261 tactctaaac aaactggtaa ctcaccagtt cacaaaggtg tatacctcaa cgaagatggt
     4321 tctgtgaact tgtctaaact tgaattcttc tcaccaggtg ctaacccatc taacaaagct
     4381 aaaggtggtt ggttgcaaaa cttgaactca ctttctagcc ttgactttag ttatgcagct
     4441 gacggtatct cattgactac acaagtatca cctcgcgctc ttggtaagac tcgtgatgaa
     4501 caagttgata acttggtaac aatccttgat ggttacttcg aaaacggtgg acaacacgtt
     4561 aacttgaacg ttatggactt gaacgatgtt tacgaaaaaa tcatgtcagg cgaagacgtt
     4621 atcgtacgta tctctggata ctgtgtaaac actaaatacc tcactccaga acaaaaaact
     4681 gaattgacac aacgtgtctt ccacgaagtt ctttcaatgg atgacgcctt ggatgcattg
     4741 agctaatcaa gttcttgaat aataaaaagg aaccctcggt caaacgactg agggttttgt
     4801 gcttgggata gtatgagcaa ttccttcggc gcaatatgca atgtttttgg gctctttgtc
     4861 aactgtagtg ggttgaaaaa aagctaagct
//
LOCUS       DAVBCW010000119         4714 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVBCW010000119 DAVBCW010000000
VERSION     DAVBCW010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.119"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(157..1905)
                     /locus_tag="VQ514_001835"
     CDS             complement(157..1905)
                     /locus_tag="VQ514_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2500564.1"
                     /translation="MKRQTVNQTLKRLVVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFADRQGNGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKELSIDIPAGSKVAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRVSLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTISRVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            complement(1907..3631)
                     /locus_tag="VQ514_001836"
     CDS             complement(1907..3631)
                     /locus_tag="VQ514_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2500565.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFLSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFAESPENIHSELEQKQVTSGRVLQV
                     QELTFTYPDAAQPSLRDISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGLNQEVTDQELWQALEIAQAK
                     DFVSDKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENLPNMSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            complement(3689..4627)
                     /gene="msrB"
                     /locus_tag="VQ514_001837"
     CDS             complement(3689..4627)
                     /gene="msrB"
                     /locus_tag="VQ514_001837"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET2500566.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
BASE COUNT         1399 a         1037 c          984 g         1294 t
ORIGIN      
        1 acctcaaagc tgtgctttga gcaacctacg gctagcttcc tagtttgctc tttgattttc
       61 attgagtatt acagtaaaca atttttctag ctattgtaac aaaaaagtca ctcgaaggca
      121 acttttttcg tccataaact ttcaaaagag aatggcttat tcagaactaa aagccgcagc
      181 tttttgcatt tggtaatact taccctttct atccatgagt tcttgatggt taccatattc
      241 aacaatatca ccatctacta agacaagaat taaatccgca tcctgaatgg ttgacaaacg
      301 gtgagcaatg atgaagcttg tgcggccctt catgagtttt gcaaaggcat cctgtaccag
      361 cacttctgtc cgtgtatcaa tggaagaagt tgcctcgtct aagataagaa tctttggaat
      421 agccagaaag actcgggata tggtcaagag ctgagcttgg ccgacagaga gagattctcc
      481 agcattttcc aacttggtat cgtatccctg tggcaactgt tggatgaaaa agtctgcatt
      541 agctgctttg gcagcagcaa ttacttgctc tcgactggct tcaggattgc caaaggcaat
      601 attatcatga atggtccctt gtgtgagcca ggtttcttga agcaccatac caaactgctg
      661 tctcaatgat actcgtgtat aatcataaat ggattgccca tccagcaaga tatctcccga
      721 gctaatggga taaaaacgca taaggagatt gataagagtt gattttccag cacctgtcgg
      781 accaacgatg gctaccttac taccagctgg aatatcgata gacaactcct taatcaaaat
      841 tttttcagga tggtagccaa aagagacatg tttaaaggaa atagctccct taacttggtc
      901 actggtcaag acttccttac ctgtttcagc cacttcaggg ctatctaaga ccccatagat
      961 acgctctacg caagccagag cactttgcaa ctcagctagc actgaagaaa tatcgttaaa
     1021 gggcttggtg tattgctgaa catagttcaa aaaagtcact aaacgaccga cggtcaaggt
     1081 tgaacccatc atgatacgat aagctcctac tccagctaaa agggcataaa tgagtgcatt
     1141 tacaaagcga gtcgaaggat tgaccgttga agaataaaag atggctgact gagaatagcc
     1201 tgagtagttg tcatgagcct cacgcaatct ttggataaat tctgtttgag cattgaagga
     1261 ctggattata gtctgctggc taagcgattc ttcaatcaac tgagtctgaa ttcccctcgt
     1321 ctctgtttgc ttctggaaga gatgatagga tctcttggca ataaagcgtg aaatcaccat
     1381 ggacagtggc gtcaacagca agactaagag agtcatgagg agatgaattt ggagcatggc
     1441 tagaatacta accaaaatca tcaaaacacc aatgaaaaat tggttaaaaa tcatggtcaa
     1501 gccagctgcc aactgttcga tatccgtggt tacacgactg accatctctc cattgccttg
     1561 ccgatccgca aaagcaatcg gtaaccgatg gagtttatgg atgattcgct ctcgtaaatc
     1621 tctggtataa gagaagatta gacgattata gaggagagga ttggcccatt gtaccagagt
     1681 atttcctatt accaccaaga gcatctggag aaaaatctgc caaaaaactg gtgatgaacc
     1741 agccactagg acttggtcaa tgacctgccc aatcagaata ggtaggtaaa ttgataagcc
     1801 aacttgggca atagttccta gaaaggctag gaaaaggagg aagggatggt ttgctaaatc
     1861 tacgactaaa cgtttgagcg tctggtttac agtttgtcgt ttcattctag tcctcctttc
     1921 catgttggga tgcattgatt tcacgataga cttgactgga tttcatcaag tcatcgtgct
     1981 tgccaacagc tagcaactca cctttttcca agaggagaat ctggtccgcc atctggagtg
     2041 tcgaagtccg ttgagaaatc aagattaagc tcatatttgg cagattttcg cggatagctt
     2101 tcaagagctt ggactctgta atggtgtcca gtgctgaggt cgcatcatct aggatgagaa
     2161 acggagcctg gcgcaagact gctcgtgcga tagacagcct ttgtttttgt ccacctgaga
     2221 aatttcgccc tcctgcttca actagagcat ccaagagtcc ttccttatca ctgacaaaat
     2281 ccttagcttg cgcaatctcc aaggcttgcc agagttcttg atcagtcact tcttgattta
     2341 aacctaaagt caagttggaa cgaatagttc ccttaaagag ttcgaccttt tgaggtacat
     2401 aggcaatcca agaccgccac tgctccaaat taagaggact acgtccattt tgataaaggt
     2461 caatgttccc cttgtctact ggataaagtc caagtaagag ttgcaccaag cttgatttac
     2521 cagaaccagt ccccccaatg ataccaagga tttgtccttg agtcatatca aaggaaatgt
     2581 ctctcagaga aggctgggcc gcatcaggat aggtaaaagt caattcttgg acttgtaaaa
     2641 cccgaccact ggtaacttgc ttttgttcta attctgaatg gatgttttcg ggagattccg
     2701 caaagacctc ctcgattctc ttggctgaga tataggactg gttgagggaa ttgatcagca
     2761 tggctagctt gaccaattcc accaaaatct gtaagaggta attgataaga gcaatgagag
     2821 caccttgact gagtactcct ccttgaattg aaatatagcc ttgccagata ataacgagaa
     2881 gagttccatt gacaatcaga taggtcagag gtgttaataa actagacaag aaacctgtct
     2941 tttcttgtaa tctagcataa acttggttaa gggtttgaaa aatctgtaac tctcgttttt
     3001 cttgaccaaa agcacgaata acccgcatcc cttgcaattg ctggcgcgtt tcctgaacca
     3061 gttggtccgt tttctttctg agactactgt agagaggatt gaccaatcga gataacccta
     3121 caatgacaat ggtcaaaatg gcaaccatga ctaagaacca gaaagtcaac tcagctgaga
     3181 ttcgataagc cataaaaatg gcaccaaaaa cgataatggg cgctcgtaaa aagagacgca
     3241 ggaattgatt gataccagtc tgaatctggt aggtatccga agtcaagcga gtaaccaaac
     3301 tagaagttgt cagacggtct ctgctgtcct ttggcaagga aagaatatga cgataaagat
     3361 cgtttgtcaa ttccttagca aaacctactg ctgcctttgc tgagtaaaat tgagctatca
     3421 aggccactaa aacgccaatt actgcaaaga taaggagcag gccaatctgc atccagagat
     3481 gaccttgatc tccctgaggt aaagattggt caacaatccc agcaatcacc atgggaacca
     3541 agagctcaaa aacagcttct aacagcttga acaagggggc taaaattgat tccttgatgt
     3601 agggtttgaa gtaagataat aggtgtttca taaatccctt ctattcatat tctagaaatg
     3661 aagaaagtgg gaagccccac tctctgtttt atttgtttaa gtaaggcaat agatagccat
     3721 atcctgcttt ttccatctca tccttggcca caaagcgtaa agaagcagaa ttgatacagt
     3781 aacggaggcc gcctaactcc cgcggtccat ctgtgaaaac atgacccaag tgagcactgc
     3841 ctgaacgaga acgaacttca attcgctcca ttccatggct cagatccttg taataatgaa
     3901 tcaactcttt ggaaatcgga cggctaaaac ttggccaacc acaacctgaa gcaaacttat
     3961 ccttggcaaa aaagagtggc tcacctgtcg taatatctac ataaatcccc tcttcaaagg
     4021 tttggtcata ggcattggta aatggagcct ctgtagcagc ttcttgtgtg acacgataag
     4081 actcttcaga tagactggcc ttcaacacct cttgactagg cttttcatag tttgctgcat
     4141 caatcaatgg cttatcagca tcggtcacat cgatatgaca gtaacctgaa ggattcttcc
     4201 tgagatagtc ttggtggtag tcttcagcca gaatgtagtg gcgtaattgc tccacttcta
     4261 ctgcaatctt tcgacccagc atgcgttcct gctcctgcac cactgtgtag atagctggca
     4321 aatctgcttc atcctgataa taaatcccag ttcgatattg gcgaccacgg tcattccctt
     4381 gttgattgat agatagagga tcgataactc ggaaataata aagtaaaatc tctctgagtg
     4441 acacttcctt ctcatcgtaa atcacttgga ccgtttctgc atggtctgtt tccttgatca
     4501 actggtaatt ggtcgtttcg acttgaccat tagcgtagcc aacactggtt tctagcactc
     4561 cagaaatgcg tgaaaaatat tcctctaggc cccaaaaaca accacctgct agataaattt
     4621 ctgccatctt tatttctcct ttatcacgtt tttaactaat actcttcgaa aatctcttca
     4681 aaccacgtca gcgtcacctt accgtaggta tgtt
//
LOCUS       DAVBCW010000120         4559 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVBCW010000120 DAVBCW010000000
VERSION     DAVBCW010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.120"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            66..230
                     /locus_tag="VQ514_001838"
     CDS             66..230
                     /locus_tag="VQ514_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HET2500567.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            309..1037
                     /gene="yaaA"
                     /locus_tag="VQ514_001839"
     CDS             309..1037
                     /gene="yaaA"
                     /locus_tag="VQ514_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HET2500568.1"
                     /translation="MKILIPTAKEMNTDLPSIEAIPLKPESQTVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(1088..2572)
                     /locus_tag="VQ514_001840"
     CDS             complement(1088..2572)
                     /locus_tag="VQ514_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2500569.1"
                     /translation="MRNLLSTKVQRQLRLMETLIQNRNWMKLHELAEKLGCTERILKS
                     DLNELRIAFPSINIQSSVNGIMIDLEVNTSVEDIYQYFLANSQSFQLLEYMFFNEGLP
                     IYRTIENLYFSSANLYRLGRNITKVLSSQFQIELSFTPSEIRGNEIDIRYFFAQYFSE
                     RYYFLDWPFPDLPEEDLTEFADFFYKITNYPMRFSIYRMYKLMIAISIHRVKNGHFID
                     LPNHFYKEYYPLLKSIPNFQETLAYFSKHFGLEMTPDTIAQIFISFLQNDIFLDPQEF
                     FNSLEDNSQARYSYQLLSQILERLSKQYKITFTNHDELIWHLHNTAFFERQEIFSTPI
                     LFEQKALTIKKFEGYFPDFMGSARQELAQYRQAIGQHDHPEQLEHLMYTILTHAENLS
                     TQLLENRPPIKVLIISNFDHAISLTFVDMLSYYCNNRFTFDIWDELKTSPEILNQTDY
                     DIIVSNFYISGITKKFICRNHLSIMNLVNHLNTLSNEIHLSNTL"
     gene            2788..>4559
                     /locus_tag="VQ514_001841"
     CDS             2788..>4559
                     /locus_tag="VQ514_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HET2500570.1"
                     /translation="MKHSHKKSFDWYSMQQRYSIRKYYFGAVSVLLGTALVLGAAASV
                     QTVQAEENKQETTNSISVGRGEAATKPAEVSASNKEKTYAAPTVANPVETTPVKTGEV
                     TKPAEKVEEAKDKKEEVTHQDAIDKSKLLTALSRAKKLESKLYTEASAANLQTSIQAG
                     QSLLGKADASEAELSAAESSIQSSIIGLELRSNSNKGTVSETPVAKKANIVEAKEETN
                     PAATTDRSAVDSAVLPTSTAAKVETTSAPASINEILKPGLSLSDARQNPAIRKEDVDR
                     GYSGFRAAPAPTNQPVTNLGNNTVFTDISRGSHTFRGNGNSRRGNPIHFDVTTTRVRN
                     RVDFSIRYSGPGEFVNNNFILDKGNGFGEPSRATVSTPKLRDQVKNISKGGNFVSHSG
                     YSLEAAVETSTNQTIKFSLPIANPNGDLSVRLRPVTFNVGQGESGSATSNDPYSNSNY
                     YFREKPLLLDANPNGGTNNKTVSEDIDFQTVYLPTSKLPEGQTRLVREGEKGQRQIIY
                     KVHQLGNETILGLPISNSVIKEVKPRIMQIGVAKDLIDTVKPRVDQNKVGDTNNLTFY
                     LDNDGNGVYTEGVDELVQKIAIKDG"
BASE COUNT         1555 a          749 c          910 g         1345 t
ORIGIN      
        1 aaaaaggttt atttaataaa tatatgttat aataaggtaa attaagccta atggaggaca
       61 aagaaatggg tttttatttg atggttgctt caatgctact cggactgctc gctttgaaga
      121 taggtttttc tcagttcaag gagaaaaaag ataaattctt atctatctta acaagtttag
      181 ctggcacagc ccttgttctc gtggctgtct ggttaggatg gcccaaataa atagaaaaac
      241 ctcggtcaaa ccgaggtttt tcaaatgaat ttcttggttg tggctaaaaa atatgctaca
      301 ctatcaatat gaaaatttta atcccaacag caaaagaaat gaacacagac ttgccaagta
      361 tcgaggcaat tcctttaaaa ccagaaagtc agaccgtgct tgatgccttg gctctctatt
      421 ctgccagtca attggagagt ttctacaagg tatcagctga gaaagcggcg gaagaatttc
      481 aaaatatcca agctttgaaa aggcaaactg ctcaacacta tccagccttg aaactttttg
      541 atgggcttat gtaccgcaac attaagagag ataagctgac cgaggcggaa caagattatc
      601 ttgaaaatca tgtcttcatt acctcggctt tgtacggtgt tgttccagcc ttgtcaccca
      661 tggctcctca ccgtttggat tttttgatga aattaaaagt cgctggtaag actttgaaga
      721 gccattggaa ggcagtctat gatgaagctc tgaagaagga agaagtgatt ttctctctct
      781 tgtcatcaga gtttgagact gtattttcta aggaaatcag agcaaagatg gtgaccttca
      841 aattcatgga ggatagaggc ggtcagctga agattcactc aactatctcc aagaaagcgc
      901 gcggggcctt tctaacagct ttaatagaaa atcaagtaca aactgtgggg gaagcacgtc
      961 gcttgaactt tgctggattt gtttaccgag aagatttgtc acaaccacag ggattggttt
     1021 ttgtaaagga agtatagaaa tcagataaga gaaaagtcag cacgattatg ctgacttttt
     1081 tgataactta taaagtattg gacaaatgaa tctcatttga taaggtgtta agatgattaa
     1141 ccaaattcat gattgacaga tgatttcggc aaataaactt cttggtaatc cctgaaatat
     1201 agaaattaga cacgatgata tcgtaatctg tctgatttaa aatctcagga cttgttttta
     1261 attcatccca aatatcgaag gtaaagcggt tattacagta ataggaaagc atatcaacaa
     1321 aggtaaggga tatagcatgg tcaaagttac tgataatcaa aaccttgata ggtggtcgat
     1381 tttctaacaa ctgagtagag aggttttcag catgggttaa gatggtgtac atcaagtgtt
     1441 ccaactgttc aggatggtca tgctgtccaa ttgcctgacg gtattgagcc agttcttgac
     1501 gcgcacttcc cataaagtct gggaagtaac cttcaaattt tttaatcgtc aatgcttttt
     1561 gttcaaataa gattggggta gaaaagattt cctgtctttc aaagaaagca gtgttgtgta
     1621 aatgccaaat cagctcatcg tggttagtaa aagtaatctt atattgtttt gataggcgtt
     1681 ctaatatttg actaagtaat tggtaagagt atcttgcttg actgttatct tctaaagagt
     1741 tgaagaattc ttgaggatct aggaaaatat cattctgaag aaaagaaata aaaatttgag
     1801 cgattgtatc tggagtcatt tcaagcccaa aatgtttaga gaaataagca agtgtctcct
     1861 ggaaatttgg aatgctcttt aaaagaggat aatattcttt gtaaaagtgg ttggggaggt
     1921 caatgaagtg accattttta accctgtgga tactgatagc tatcatcagt ttgtacattc
     1981 tataaattga aaatcgcatt ggataattcg tgattttata gaagaagtca gcaaactctg
     2041 ttaaatcttc ctcaggaagg tcaggaaaag gccaatctag gaaataatag cgctcagaaa
     2101 agtattgggc aaaaaagtaa cgaatatcga tctcatttcc acgtatctcg gatggagtaa
     2161 aagataattc aatttgaaac tgagatgaaa gaacctttgt aatatttctg cccagtcgat
     2221 agaggttggc actactaaag taaagatttt ctattgttcg ataaataggt agcccctcat
     2281 tgaagaacat atactctagt aattgaaaag attgagaatt agcaagaaaa tattgataaa
     2341 tatcttctac actagtgtta acttctaaat ctatcatgat tccgttaaca gaagattgga
     2401 tgttaataga tggaaaggca atacgtaatt catttaaatc actttttaag attcgttctg
     2461 tacatcctaa tttttcggct agttcatgta atttcatcca attacgattc tgaattaggg
     2521 tttccattaa gcgtaattgt ctttgaactt ttgtggataa aaggtttctc atacgggtat
     2581 ttcctccata attcattata tctttttttg tctaagtagt ataaaaaaat tagagcaaga
     2641 aaattgtcgt tagtaatcgg aaaaattaga ataatataag tggcttttaa gtgcaaaaac
     2701 agaatatatt aattgttcag aaaatattga ttaatgcctc aaatagttgt acattgtaaa
     2761 ctataaaata ctatctagga gtttaaaatg aaacattcac ataaaaaatc atttgactgg
     2821 tatagcatgc aacaacgtta ttctattcgt aagtattact ttggtgcagt tagtgtcttg
     2881 ctcggtaccg ctttggtatt aggtgcagca gctagtgtcc aaacagtaca agcggaagaa
     2941 aacaaacaag aaactaccaa tagtatttct gttggtaggg gagaagcagc tactaaacca
     3001 gcagaggttt ctgcgtctaa taaagagaaa acctatgcag ctccaactgt agctaatcca
     3061 gtagaaacga ctccagttaa aactggagaa gttactaaac cagcagaaaa agttgaagaa
     3121 gcaaaagaca aaaaagagga agtaacgcat caagatgcta ttgacaagtc aaaattgtta
     3181 acggctcttt cgcgtgctaa aaagttagaa agcaagttat atacagaagc aagtgctgct
     3241 aacttgcaaa caagtatcca agctggtcaa agcttgcttg ggaaagcaga tgcatctgaa
     3301 gctgaattat cagcagcaga gtcatctatt caatcatcta ttattggtct agaacttcgt
     3361 tctaactcta acaaaggaac tgtatcagaa actcctgtag cgaagaaagc taatatagtt
     3421 gaagcgaagg aagagactaa tccagctgca acaactgatc gttcagctgt tgatagtgct
     3481 gttttgccaa ctagcacagc tgccaaagta gaaacaactt cagctccagc atctattaat
     3541 gaaatcttga aaccaggttt gagcctttct gatgctcgcc aaaatccagc tatccgtaag
     3601 gaagatgttg atagagggta tagtggtttt agagcggcgc ctgctccaac gaaccaacca
     3661 gtaactaatc taggaaataa tactgttttc actgatatta gcagaggttc tcatactttc
     3721 cgtggtaatg gaaattcacg tcgtggaaac ccgattcatt ttgatgtaac aacaactaga
     3781 gttagaaata gagtagattt ctcaatcaga tattcaggac caggtgagtt tgttaataac
     3841 aactttatct tagacaaagg aaatggattc ggagaacctt ctcgtgcaac tgtttcaaca
     3901 ccaaaattaa gagaccaagt taaaaacatt agtaagggtg gaaactttgt ttctcattca
     3961 ggctacagtt tagaagctgc agtagaaaca agcaccaatc aaacaattaa gtttagttta
     4021 cctatagcca accctaatgg tgatctgtct gttcgattaa gacctgttac ttttaatgta
     4081 ggtcaagggg aatcaggatc tgcaactagt aatgatccat atagtaactc taactattac
     4141 ttcagagaaa aaccactcct cttggatgca aatccaaatg gtggtactaa taataagact
     4201 gtttcagaag acattgactt ccaaactgtc tatcttccaa ctagtaagtt accagaaggt
     4261 caaactagat tagttcgaga aggtgaaaaa ggacaacgtc aaatcatcta taaagttcat
     4321 caattgggta acgaaacaat cctaggattg ccaattagta attcagttat taaagaagtt
     4381 aagccacgta ttatgcaaat tggtgtggct aaagatctaa tcgatacagt aaaaccacgt
     4441 gttgatcaaa ataaagtcgg tgatacaaat aacctcactt tctatcttga taacgatgga
     4501 aacggtgttt atactgaggg cgtagacgaa cttgttcaaa aaattgctat taaagatgg
//
LOCUS       DAVBCW010000121         4500 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVBCW010000121 DAVBCW010000000
VERSION     DAVBCW010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.121"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            131..1390
                     /locus_tag="VQ514_001842"
     CDS             131..1390
                     /locus_tag="VQ514_001842"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET2500571.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            1406..1957
                     /locus_tag="VQ514_001843"
     CDS             1406..1957
                     /locus_tag="VQ514_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2500572.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLFIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            1950..3227
                     /locus_tag="VQ514_001844"
     CDS             1950..3227
                     /locus_tag="VQ514_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET2500573.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIDRLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            3243..4103
                     /locus_tag="VQ514_001845"
     CDS             3243..4103
                     /locus_tag="VQ514_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HET2500574.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
BASE COUNT         1353 a          724 c         1037 g         1386 t
ORIGIN      
        1 attcagttac taactcgtca actctgattt atccaataaa attgaaaagg atggaaaaaa
       61 ggataaattt atgatatact ttattttgaa gaccttatta gaaatcttga aagagtattg
      121 aaaacttaga atgagaaaaa ttgttatcaa tggtggatta ccactgcaag gtgaaatcac
      181 tattagtggt gctaaaaata gtgtcgttgc cttaattcca gctattatct tggctgatga
      241 tgtggtgact ttggattgcg ttccagatat ttcggatgta gccagtcttg tcgaaatcat
      301 ggaattgatg ggagctactg ttaagcgtta tgacgatgta ttggagattg acccaagagg
      361 tgttcaaaat attccaatgc cttatggtaa aattaacagt cttcgtgcat cttactattt
      421 ttatgggagc ctcttaggcc gttttggtga agcgacagtt ggtctaccgg gaggatgtga
      481 tcttggtcct cgtccgattg acttacacct taaggcgttt gaagctatgg gtgccactgc
      541 tagctacgag ggagataaca tgaagttatc tgctaaagat acaggacttc atggtgcaag
      601 tatttacatg gatacggtta gtgtgggagc aacgattaat acgatgattg ctgcggttaa
      661 agcaaatggt cgtactatta ttgaaaatgc agcccgtgaa cctgagatta ttgatgtagc
      721 tactctcttg aataatatgg gtgcccatat ccgtggggca ggaactaata tcatcattat
      781 tgatggtgtt gaaagattac atgggacacg tcatcaggtg attccagacc gtattgaagc
      841 tggaacatat atatctttag ctgctgcagt tggtaaagga attcgtataa ataatgttct
      901 ttacgaacac ctggaagggt ttattgctaa gttggaagaa atgggagtga gaatgactgt
      961 atctgaggac agcatttttg tcgaggaaca gtctaatttg aaagcaatca atattaagac
     1021 agctccttac ccaggctttg caactgattt gcaacaaccg cttacccctc ttttactaag
     1081 agcgaatggt cgtggtacaa ttgtcgatac gatttacgaa aaacgtgtaa atcatgtttt
     1141 tgaactagca aagatggatg cggatatttc gacaacaaat ggtcatattt tgtacacggg
     1201 tggacgtgat ttacgtgggg ccagtgttaa agcgaccgac ttaagagctg gggctgcact
     1261 agtcattgct gggcttatgg ctgaaggtaa aactgaaatt accaatatcg agtttatctt
     1321 acgtggttat tctgatatta tcgaaaaatt acgtaattta ggagcggata ttagacttgt
     1381 tgaggattaa accgtagagg tgtttatgaa tatttggacc aaattagcaa tgttttcttt
     1441 ttttgaaacg gatcgcttgt atttgcgtcc tttctttttt agtgatagtc aggacttccg
     1501 cgagatagct tcaaatccag aaaatcttca atttattttc ccaacgcagg caagtctgga
     1561 agaaagtcaa tatgcactgg ccaattactt tatgaagtcc cctttgggag tgtgggcaat
     1621 ttgtgaccag aaaaatcaac aaatgattgg ttctattaaa tttgagaagt tagatgaaat
     1681 caaaaaagaa gctgagcttg gctatttttt gagaaaagat gcttggtcgc aaggatttat
     1741 gacagaggtt gttagaaaaa tttgtcagct ttcttttgag gaatttggct taaaacaatt
     1801 atttatcatt acccaccttg aaaatgaagc tagccaaaga gttgctctta agtctggatt
     1861 tagtttgttc cgtcagttta agggaagtga tcgttacaca agaaaaatgc gggattatct
     1921 tgaatttcgg tatgtaaaag gagagttcaa tgagtaagca tcaggaaatt ctaagctatt
     1981 tggaggaatt accagtaggt aaaagggtca gtgttcgtag catttcgaat catctaggag
     2041 ttagtgatgg aacagcctat cgagctatta aagaagctga aaaccgtgga attgtggaga
     2101 cccgtcctag aagtggaaca attcgtgtta aatcccagaa agttgctata gatagattaa
     2161 cgtttgctga aattgcagaa gtgacttctt ctgaggttct ggctgggcaa gaaggtttag
     2221 agagagaatt tagtaagttt tcaattggtg ccatgactga acaaaatatc ttgtcttacc
     2281 ttcatgatgg ggggctcttg attgtcggag accgaacccg tattcagttg ctagccttgg
     2341 aaaatgaaaa tgcagttctg gttacagggg gatttcaggt tcatgatgat gtgcttaaac
     2401 tggccaatca aaaagggatt cctgttctaa gaagtaagca tgataccttt accgtcgcta
     2461 ccatgatcaa taaagccttg tcaaatgtcc aaatcaagac tgatattctg acagttgaga
     2521 aactttatcg ccctagtcat gagtatggtt ttctgagaga gacagataca gttaaagatt
     2581 atttggactt ggttcgtaag aatcgtagca gccgtttccc tgttatcaat caacatcagg
     2641 tcgttgttgg cgttgtaacc atgagagacg ctggtgataa atcaccaagc acgacaattg
     2701 ataaggttat gtctcgtagt ctatttttgg ttggattatc gacaaatatt gccaatgtga
     2761 gtcaacggat gatcgcagaa gactttgaaa tggtaccagt tgttcgaagc aatcaaactt
     2821 tgcttggcgt tgtgacgcga cgagatgtca tggagaagat gagccgttcc caagtttcgg
     2881 ctctaccaac tttttctgag cagattggac aaaagctctc ttatcaccat gatgaagtag
     2941 tcattacagt ggaacccttt atgctagaaa aaaatggagt tttggctaat ggtgtattgg
     3001 cagaaattct gacccacatg acccaagatt tagttgttaa tagtggtcgc aatctcatta
     3061 tcgagcagat gctgatctac tttttgcagg ccgttcagat agatgatata ttgcgcattc
     3121 aggcacggat tattcatcat acgagacggt cagctataat tgattacgat atttatcatg
     3181 gtcaccagat tgtttcaaaa gcaaatgtga ctgttaaaat taattagaaa ctaggagaaa
     3241 agatgataac attaaaatca gctcgtgaaa tcgaagctat ggacaaggct ggtgattttc
     3301 tagcaagtat tcatataggc ttacgtgatt tgattaagcc aggcgtagat atgtgggaag
     3361 ttgaagaata tgtccgccgt cgttgtaaag aagaaaattt ccttccactt cagattgggg
     3421 ttgacggtgc catgatggac tatccttatg ctacctgttg ctctcttaac gatgaagtgg
     3481 ctcacgcttt ccctcgtcat tatatcttga aagatggtga tttgctcaaa gttgatatgg
     3541 ttttgggagg tcccattgct aaatctgacc taaatgtctc aaaattaaac ttcaacaatg
     3601 ttgaacaaat gaaaaaatac actcagagtt attctggtgg tttagcagac tcatgttggg
     3661 cttatgctgt tggtacaccg tccgaagaag tcaaaaactt gatggatgta accaaagaag
     3721 ctatgtacaa gggtattgag caagctgttg ttggaaatcg tatcggtgat atcggtgcgg
     3781 ctattcaaga atacgctgaa agtcgtggtt acggtgtagt gcgtgatttg gttggtcatg
     3841 gtgttggccc aactatgcac gaagaaccaa tggttcctaa ctatggtatt gcaggtcgtg
     3901 gactccgtct tcgtgaagga atggtcttaa ccattgaacc aatgatcaat acaggcgatt
     3961 gggaaattga tacagatatg aaaactggtt gggcgcataa gaccattgac ggtggattgt
     4021 catgtcagta tgaacaccaa tttgtcatta cgaaagatgg acctgttatc ttgactagcc
     4081 aaggtgaaga aggaacttat taataaaaag tgaaaagact actggaagtt tattatgaaa
     4141 aaatccagta gatcttttca taataaaacg cattgtatca agtgttaggg gctgatatca
     4201 tgcgtttttc tgcttttaag attttttcca actctgtttg taagcgcatc ataacaaagg
     4261 gtctaggatt cagggctctc ctcctatata ctattagtaa agtaaaacta agggaggata
     4321 ttttagtgtc gcagtctatt gttcctgtag agattccaca atattgtcgt tttgattcta
     4381 aaaagagaaa tggaattctg tttaatgttc gtattgccaa tcttaaattt acctttttta
     4441 gattagactt cctgcgaaac aaaacatggt atagtagttc tatgaatgat gaagcaagta
//
LOCUS       DAVBCW010000122         4476 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVBCW010000122 DAVBCW010000000
VERSION     DAVBCW010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.122"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            <1..239
                     /locus_tag="VQ514_001846"
     CDS             <1..239
                     /locus_tag="VQ514_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500575.1"
                     /translation="TGWAKVNGSWYYLNANGSMATGWVKDGDTWYYLEASGAMKASQW
                     FKVSDKWYYVNGLGALAVNTTVDGYKVNANGEWV"
     gene            640..1761
                     /gene="mnmA"
                     /locus_tag="VQ514_001847"
     CDS             640..1761
                     /gene="mnmA"
                     /locus_tag="VQ514_001847"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HET2500576.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRRLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            1902..2357
                     /locus_tag="VQ514_001848"
     CDS             1902..2357
                     /locus_tag="VQ514_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2500577.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALIVQNHKLLVTKDKGKYYTIGGA
                     IQVNESTEDAVVREVKEELDVKAQAGQLAFVVENRFEVDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLQNIQLVPAFLKTALPDWEGQLRHIHLEE"
     gene            2367..4280
                     /gene="mnmG"
                     /locus_tag="VQ514_001849"
     CDS             2367..4280
                     /gene="mnmG"
                     /locus_tag="VQ514_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HET2500578.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEVPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
BASE COUNT         1291 a          907 c         1092 g         1186 t
ORIGIN      
        1 ctacaggttg ggctaaagtc aacggttcat ggtactacct caacgctaac ggttcaatgg
       61 caacaggttg ggtgaaagat ggagatacct ggtactatct tgaagcatca ggtgctatga
      121 aagcaagcca atggttcaaa gtatcagata aatggtacta tgtcaatggt ttaggtgccc
      181 ttgcagtcaa cacaactgta gatggctata aagtcaatgc caatggtgaa tgggtttaag
      241 ccgattaaat taaatcatgt taagaacatt tgacatttta attttgaaac aaagataagg
      301 ttcgattgaa tagatttatg ttcgtattct ttaggtacct atcttatgat ttcaggaaat
      361 gtcattaaaa aaacgactcc ttttctctaa cttgaaaaat agattagaga aaatgggttg
      421 ttttatttat tatagttatt tgaatgaaga taagaagaag gtatactcac atcattcaca
      481 taatctgtat gttgactata agtttaaaaa aacaattttt aagctcttcc ttgtcttctc
      541 caaccaagcg tgttataatg aatactgctc aagcgacctt caatcgtgaa gcacacacga
      601 ccttcaatcg tgaataaacg aatagatggg agacttacca tgagtgataa ctctaaaaca
      661 cgtgttgtcg tggggatgag tggtggtgtt gattcgtcgg tgacggctct tttgctcaag
      721 gagcagggct acgatgtgat cggtatcttc atgaagaact gggatgacac agatgaaaac
      781 ggcgtctgta cggcgaccga agattacaag gatgtggttg cggtggcaga ccagattggc
      841 attccctact actctgtcaa ttttgaaaaa gagtactggg accgcgtttt tgagtatttc
      901 ctagcggaat accgtgcagg gcgcacgcca aatccggacg ttatgtgcaa caaggaaatc
      961 aagttcaagg cctttttgga ctatgccatg accttggggg cagactatgt agcgactggg
     1021 cattatgctc gagtggcgcg tgatgaggac ggcactgttc acatgcttcg tggcgtggac
     1081 aatggcaagg accagaccta tttcctcagc caactttcgc aagaacaact ccaaaaaacc
     1141 atgttccctc tgggacattt ggaaaagcct gaagtacgca gactagcaga agaagcaggc
     1201 ctttcgactg ctaagaagaa agactcgaca gggatttgct ttatcggaga aaagaacttt
     1261 aaaaactttc tcagcaacta cctgccagct cagcctggtc gcatgatgac tgtggatggt
     1321 cgcgatatgg gcgagcatgc aggtcttatg tactatacaa tcggtcagcg tggcggactc
     1381 ggtatcggtg ggcaacacgg cggtgacaat gccccttggt tcgttgtcgg aaaagatcta
     1441 agcaagaata ttctctatgt aggacaagga ttctaccatg attcgctcat gtcaactagc
     1501 ctagaagcca gtcaagtcca ctttactcgt gaaatgccag aagagtttac gctagaatgt
     1561 acggctaaat tccgttaccg tcagcctgac tctaaggtga ccgtccatgt caaaggagac
     1621 aaggcagaag ttatctttgc ggaaccacaa cgcgcgatta caccaggaca ggcagttgtc
     1681 ttttacgatg gcgaagagtg tctaggtggc ggtttgattg acaatgctta ccgcgatgga
     1741 caagtttgtc agtacattta gattgacaaa ttttctcaat ttgctacaat aataacagca
     1801 atagaaatga tggtcaaagc tcatggatgt tgcaggcttt tttgtcctgc acttctttgt
     1861 agttttgact gtttttgtgt cgtttaaggg aaaggacaag aatgactcaa caagactttc
     1921 ggacaaaagt agacaatacg gtttttggtg ttcgggctac agccttgatt gtccaaaatc
     1981 acaagcttct agttaccaaa gacaagggca agtattacac tattggaggt gcgattcaag
     2041 tcaatgaaag cacggaagac gcggtagtcc gtgaagtgaa ggaagaactg gatgtcaaag
     2101 ctcaagctgg gcagctagct tttgtggttg aaaatcgttt tgaagtggac ggcgtttcct
     2161 atcacaatat cgagtttcat tatctggtgg atttgcttga agatgccccg ttgaccatgc
     2221 aggaagatga gaaaaggcag ccctgtgagt ggattgactt ggataagctc cagaatatcc
     2281 agctagttcc agccttttta aaaacagccc taccagattg ggaaggccaa ctaagacaca
     2341 ttcatcttga ggaataggag agaaacatga cttatcattt tactgaagaa tacgatatta
     2401 ttgtaattgg tgcgggacac gctggggttg aggcttcctt ggccgctagc cgtatgggct
     2461 gtaaggtcct gcttgcgacc atcaatattg aaatgctggc tttcatgcct tgtaatccct
     2521 ctatcggtgg ttctgctaag gggattgtcg tacgtgaagt cgatgccctc ggtggcgaga
     2581 tggccaagac cattgacaag acttacatcc agatgaagat gctcaacaca gggaagggac
     2641 cagccgttcg tgcccttcgt gcgcaggctg ataaggaact ttactctaag gaaatgcgca
     2701 agacagttga aaatcaagaa aatctgaccc ttcgtcaaac catgattgat gagattttgg
     2761 tggaagatgg caaggttgtc ggtgtgcgta cagccaccca tcaagaatat gctgctaagg
     2821 ctgttattgt gacgacaggg actgctctcc gtggggaaat tatcatcgga gacctcaagt
     2881 actcatcagg tcctaaccac agcttggctt ctattaacct agctgacaat ctcaaggaac
     2941 tgggtctcga aatcggtcgt ttcaagacag gaacccctcc acgtgtcaag gcttcttcta
     3001 tcaattacga tgtgacggaa attcagccag gagacgaagt gcctaatcat ttctcataca
     3061 cttcacgtga tgaggattat gtcaaagatc aagtgccatg ctggttgacc tataccaatg
     3121 gtaccagtca tgagattatc caaaacaacc tccaccgtgc gcctatgttt acaggtgtgg
     3181 tcaagggagt ggggcctcgt tactgtccgt cgattgaaga caagattgtg cgctttgcgg
     3241 acaaggaacg tcaccaactc ttccttgagc cagaagggcg caatactgag gaagtctatg
     3301 ttcaaggact ttcaaccagt ctgcctgagg atgtccagcg tgacttggtt cattccatca
     3361 aaggtttgga aaatgcagag atgatgcgga caggttatgc tattgagtat gatatggtct
     3421 tgcctcatca gttgcgtgcg actttggaaa ccaagaaaat ctcaggtctc ttcactgctg
     3481 gtcagacaaa tggaacatca ggttatgaag aagctgctgg ccaagggatt atcgcgggta
     3541 tcaatgcggc tctgaaaatc caaggtaaac ctgagttgat tctaaaacga agtgacggtt
     3601 atatcggggt gatgatcgac gacttggtga ccaagggaac cattgaacct taccgtctct
     3661 tgaccagtcg tgctgaatac cgtctcattc ttcgtcatga caatgctgat atgcgcttga
     3721 ctgagatggg acgcgagatt ggccttgtgg atgatgaacg ctgggctcgt tttgaaatca
     3781 agaaaaatca atttgataat gagatgaaac gcctagacag tatcaaactc aagccagtca
     3841 aggaaaccaa tgctaaggtt gaggaaatgg gcttcaagcc gttgacagat gcggtgacag
     3901 ccaaggaatt ccttcgccgt ccagaagttt cttaccaaga tgtggtggcc ttcatcggac
     3961 cagctgcaga agacttggat gacaagatta tcgaattgat tgaaacagaa atcaagtacg
     4021 aaggctatat ttccaaagcc atggatcagg ttgctaagat gaaacgtatg gaagaaaaac
     4081 gcattccagc caatatcgac tgggatgaca tcgattctat tgcgacggaa gctcgtcaga
     4141 agttcaaact catcaatcca gaaaccatcg gccaagccag ccgtatttcg ggagtaaacc
     4201 cagcagatat ttctattttg atggtgtatc tggaaggtaa aaatcgtagt atttctaaaa
     4261 ctcttcaaaa atcaaaatga tacgtcgtcg gcttcttacg aatgagttca aagcttggct
     4321 ttgattcatc tccaacctcc catagttccc cgaactatgg gagctaactc aagttatgca
     4381 ttttcctagc cttctggtct gattttcatt gagttttaat ctcaaaagaa agcatagaga
     4441 aaaacagctc cgaagacggg gctgttttgt tgactt
//
LOCUS       DAVBCW010000123         4438 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVBCW010000123 DAVBCW010000000
VERSION     DAVBCW010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.123"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            <1..119
                     /locus_tag="VQ514_001850"
     CDS             <1..119
                     /locus_tag="VQ514_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2500579.1"
                     /translation="QQGFTDRLVGYSSSIALVFTTLIGMISLVQRRVLKEDD"
     gene            121..966
                     /locus_tag="VQ514_001851"
     CDS             121..966
                     /locus_tag="VQ514_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2500580.1"
                     /translation="MKRFKPTTILEYILLTLLAGLFLFPMVWMVVSAMKPEADVYKNL
                     TSWQAFLPSLNPADWFKPYQEVFERFSIFTYLGNSIFYAGTFAIGSIIVNALAGFAFA
                     KVNFSGKKILFGFLLALLIIPVETVLIPQFTIINSLGLVNNRLAVIIPGLASVFNIYL
                     FRNFFIAIPEEILESAKMDGASIVRIFFRIMLPMSKPAVATVGVLSFISSWNDYIWPL
                     MVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTISTIPLILVYVVAQKYIVQGLGGS
                     GTGIK"
     gene            976..2295
                     /locus_tag="VQ514_001852"
     CDS             976..2295
                     /locus_tag="VQ514_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HET2500581.1"
                     /translation="MINKTFTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEETGVRQVQNMAFSDDGIHFEKISQNPVATGSDLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKDNVGCIVLLGSDNLVEWQFESIFLKGGEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWREKRFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDTDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(2503..2575)
                     /locus_tag="VQ514_001853"
     tRNA            complement(2503..2575)
                     /locus_tag="VQ514_001853"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2540..2542),aa:Lys,seq:ctt)
     gene            complement(2839..4110)
                     /locus_tag="VQ514_001854"
     CDS             complement(2839..4110)
                     /locus_tag="VQ514_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HET2500582.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYAARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQRIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWIKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDRLRKI"
BASE COUNT         1294 a          809 c          976 g         1359 t
ORIGIN      
        1 atcaacaagg ttttactgat cgtcttgtag gatattctag ctcaatcgct ttagtattta
       61 caacgttaat tggtatgatt agcttagtac aacgtcgcgt tctgaaggag gatgactaag
      121 atgaaacgat ttaaaccaac aacaatctta gaatatattc tgttaacttt attggcagga
      181 ctcttccttt ttcctatggt ttggatggta gtttcagcta tgaagccaga agcggatgtt
      241 tataaaaatt tgactagttg gcaagccttc ttaccaagtt taaatcccgc tgattggttt
      301 aaaccttatc aggaagtatt tgagcgcttt agtattttta catatctagg gaacagcatt
      361 ttttatgctg gaacgtttgc tattggttct attattgtca atgcactggc cggtttcgct
      421 tttgcaaaag tcaatttttc aggtaagaaa attttatttg gtttcttact tgctttactt
      481 attattccag tggaaactgt gttaattcca caatttacca ttatcaattc cttaggtttg
      541 gtcaataatc gtcttgcagt tattattcca ggattggcga gtgtatttaa tatttactta
      601 tttaggaatt tctttattgc aattccagaa gagattcttg agtctgcaaa gatggacgga
      661 gcaagtatcg ttagaatttt ctttagaatt atgcttccaa tgtctaaacc tgcagttgct
      721 acagttggtg ttttatcatt tatttcaagc tggaatgatt atatctggcc attgatggtt
      781 ttgacagatt cttctaaatt ttcaatgcag gttgcaatta caactattaa tacaactcaa
      841 ccagtttata tcaatcaggt aatggctgta ttaactattt caacgattcc gttgattttg
      901 gtctatgtag tcgctcagaa atatattgtt caaggtctgg gtggttccgg aactggaata
      961 aaataggata tacttatgat aaataaaaca tttacagtag aaaaagccaa tcgttttata
     1021 gcagaaaata aacatctcgt taatactcaa tataagcctg aagaacattt ttcagctgag
     1081 attggttgga tcaatgatcc aaatggattt gtctattttc gtggagaata ccatctcttt
     1141 tatcaattct atccatatga tagtgtttgg gggcctatgc actggggaca tgctaaaagt
     1201 aaggacttgg tgacttggga gcacttgcca gtggcacttg ctcctgacca agattatgac
     1261 cgaaatggtt gtttctcagg ctctgccatt gtcaaggatg atcgcctctg gctcatgtac
     1321 actggacata tcgaagaaga aaccggtgtc cgccaagtgc aaaatatggc attttcagat
     1381 gacgggattc actttgaaaa gatttcccaa aatccagttg caactggatc agacttgcca
     1441 gatgagttga ttgctgctga tttccgtgat ccaaaactct ttgaaaaaga tggacgctat
     1501 tactccgtag tagctgccaa acacaaggat aatgtgggct gtatcgttct actagggtcc
     1561 gataacctag tagaatggca gttcgaatcc atctttttaa aaggaggaga acaccaaggc
     1621 tttatgtggg aatgcccaga ttacttcgag ttagatggga aagattgcct tattatgtca
     1681 cccatgcgtt atcagcgtga gggagactca tatcataaca tcaactcatc gcttttgttc
     1741 acgggtaagg tagattggag agaaaaacgt tttatcccag aatcagttca agaaattgat
     1801 catggccaag acttctatgc gcctcaaaca ttgttggacg atcaaaatcg tcgtatcctg
     1861 attgcttgga tgcagacatg ggggcgtacc cttccaaccc atgaccaaga acacaaatgg
     1921 gcatgtgcca tgactttacc tagaattcta agattggaag atggcaaact aagacaattc
     1981 cctgttaaaa aaggccaata tcaaatccaa atagataaag attgtcatta ccacttagga
     2041 aatgatacag attatcttga atttggttat gacagtaatg cgcagcaagt ttacattgat
     2101 cgtagccatc ttattcaaaa aattctaggt gaagaagaac aggacactag tcgacggtat
     2161 gtagatattg aagctaaaga attggaagtt gttctagata aaaattccat cgagattttt
     2221 gtcaatcaag gtgaagcaag cttgactgca acttattact taacggtgcc agctgagcta
     2281 tcacgaattg attaaaaatt aagttatttc tcctaaagaa aaagttctct ttctaaaata
     2341 gtggaaagag gactttttgt gttttgggta tataagctta gtttatggta tttgtaaaat
     2401 tggtgttgga ttatgattta agctagtttt ctaaagaatt tgaaaaaaat tttatttaag
     2461 caaaaaaacc ttggttccaa ggcttttcct gttgtattta gatgccccct acagggatcg
     2521 aacctgtgac ccacggatta agagtccgct gctctgccag ctgagctaag gaggcaaaga
     2581 aaaagctgta ttggtgccga aacttcacgg tttgtattga acccgcgcaa ttaagcaggt
     2641 gggcaactcg ctctaactga agctgtttcc gtgtgagacg gcctacatgc tgttagaaga
     2701 cttttgtttc cctaataata caaaaaatag tcggtcaaca cttaagtgtg aagtcgtaca
     2761 ccacagcgtt tctatactta tatgatacca ctttttcaaa aaaaatcaag aggaaagtgc
     2821 aattttttga aaaggatttc agatttttcg caaacgatcg atagcttcct ttctttgagc
     2881 caaagcccgt tcatatttac cagtatcttc tgcttggaaa tagtgatgat tacgaatttt
     2941 ttctggcaga tagtcttgct tgatccaatt tccaggatag ttgtgtggat agagatagtc
     3001 ttgggcattc cccagttcct tgcttccact gtagtgccca tcacgcaggt gtcgcggaat
     3061 aggcaagtgc cctgatgttt tgaggtcagc aagtgcctta tccatagcta cataggctga
     3121 gttggatttt ggagaaaggg ccaaatcaat cacgacattg gcaatgagaa tgcgggcttc
     3181 ggggaaacca atcctttgtg cagcatccag agcagtcacg gtatgaatct gggcctcagg
     3241 attggctaag ccaatatctt cataggcgat aacagtcaag cgacgagcga gactaggcag
     3301 atccccagcc tcaatcaagc gggcagcata gtggagactg gcatccacat ctgagccacg
     3361 gatagacttt tgcagggcag atagaacatc atagtgaccg tctccatcct tgtccatagt
     3421 gatgtagctt ctctgaagac tattttccat gatgtctaag gtgatgtggc gaatgccctc
     3481 gtcattctca ggggtagaga gaacagccaa gtccagtgag ttaaaggcag agcgaaggtc
     3541 tccgtttgta gaggttgcga tgaaatccag cgcatcctca tctagttcta ttggaaaatc
     3601 aaaaccacgt tcagggttac ttagagctat ctgcagggcc tctttgacgt cttggttaga
     3661 cagaggttcc aactcgaaaa tttgaacacg gctacgaatg gcaggagtga cagagaagaa
     3721 aggattttca gtcgttgctc caatcatgat gaccagtcca ctttccaaga gaggcaagag
     3781 gaagtcttgc ttggtcttat ctagtcgatg aatttcgtct agcaatagga cgagaccacc
     3841 agaaaattta gcttcttccg agatttcttg cagtcgcttt ttactatcaa ctgtcgcatt
     3901 gaaagttcga aaggcatact tggtcgttcc agcgatggca gaggcaatac tggttttgcc
     3961 gattccagga gggccatata gaatcatgga ggacaggcgg ttggcttcca ccatgcggcg
     4021 gatgattttt ccaggtccga ccagatgctc ctgaccgatg acctggtcga tggttttagg
     4081 gcgcatgcga agcgcgagat tgtctggcat agcagtcctt tctaacgtgg atttgagtag
     4141 aatgactcat ctactatatc ttactatgtc cattctagcc tattagttgt gatatttcaa
     4201 tggttttgta ataatcaggg agtgagatag aatctttttt actatttaat ttaatgagga
     4261 gaggtatcag catgagagat ttgattttcc ttaaagctca aaataggata gttgagccta
     4321 aagaagtgga agtgctgact tgagaaataa tattgctgaa tgtcattgta tgaactataa
     4381 tgaaaagaga ggtgataagc aatggaaatg atggaccaag tcaggaaatt ttgatttt
//
LOCUS       DAVBCW010000124         4393 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVBCW010000124 DAVBCW010000000
VERSION     DAVBCW010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.124"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(26..1036)
                     /locus_tag="VQ514_001855"
     CDS             complement(26..1036)
                     /locus_tag="VQ514_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HET2500583.1"
                     /translation="MSFLSKNGAGILACLLISIVSWYLGGFFPVIGAPVFAIFIGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVVLLGFGLNISQVFAVGQSSLPVILSTISIALI
                     IAYLFQRFFALDTKLATLVGVGSSICGGSAIAATAPVIDAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLT
                     RTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKELSKFLIVMAMSAIGLKTNLVAMVKSSGKSILLGAICWIAIILTTLGMQTLIG
                     IF"
     gene            1185..2354
                     /locus_tag="VQ514_001856"
     CDS             1185..2354
                     /locus_tag="VQ514_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET2500584.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQVISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPAAFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            2351..3121
                     /gene="recO"
                     /locus_tag="VQ514_001857"
     CDS             2351..3121
                     /gene="recO"
                     /locus_tag="VQ514_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HET2500585.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            3118..4110
                     /gene="plsX"
                     /locus_tag="VQ514_001858"
     CDS             3118..4110
                     /gene="plsX"
                     /locus_tag="VQ514_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HET2500586.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLRGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            4116..4349
                     /locus_tag="VQ514_001859"
     CDS             4116..4349
                     /locus_tag="VQ514_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET2500587.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQSVGDVVKIIQGK"
BASE COUNT         1276 a          836 c         1127 g         1154 t
ORIGIN      
        1 ggtttgaaga gattttcgaa gagtgttaga aaatgccgat aagggtctgc ataccaagag
       61 tggtgaggat gatggcaatc cagcagatgg ctccaagaag aatggatttt ccactggatt
      121 tgaccatagc gaccagattg gttttgagac cgatagcact catggccatg acaataagga
      181 atttagagag ttctttgaga ggggtaaaga aactactgga cacacctaga gaggtgagta
      241 gtgtagtgag gagagaggca aggataaagt aaaggataaa aagtgggaag acttttttca
      301 gttgtaggct ttgcttgttt tcttgttggc gactttgcca gtaggagaga aagagcgtga
      361 tagggataat agccaaagta cgtgtgagtt taacaatggt tgcagactcg agggtattgc
      421 tttggtaaag actgtcccaa gcgctggcgg cagccgttac agaggaagtg tcgttgaccg
      481 cagtccctgc aaagagggcg aagccttcat tggatagatg aagccaggtg ccgagggttg
      541 gaaagatgag cgcagccaag acattgaaga aaaagataac ggaaatggct tgggctactt
      601 ccttttcctt agcatcaata acgggcgctg tcgctgcaat ggcagaaccc ccacagatag
      661 aagaacctac tccaaccaag gtagccagtt ttgtatccag tgcaaagaaa cgctggaaga
      721 ggtaggcaat aatcagagct attgagatag tggacaggat gacagggagt gaagattggc
      781 caactgcgaa gacctgcgag atattgagac caaaaccaag caagacaacg gcatattgga
      841 gcaacttctt ggaactaaag gtcaaaccag catccagttg tttataggac gagagaaagg
      901 gatgtaggag catgcctatg aaaatggcaa aaacgggcgc gccaatcaca gggaagaatc
      961 ctcctaagta ccaagatacg atagaaatga gaaggcaggc caagatacct gctccatttt
     1021 ttgatagaaa tgacataaaa acctccaaat aaaatctatt accattatag acctataaac
     1081 aggaaaagta aaatagaaag tggaaagcta ttctctaatg tatttttgcg gtcggggggc
     1141 ttttgtagta taatagagat acgttttgaa agtaggaggt atctatggac ttaactaagc
     1201 gctttaataa acagttagat aaaattcaag tttcgttgat tcgtcagttt gaccaggtta
     1261 tttcggagat tcctggggtc ttgcgtttga ccttggggga acctgatttt acaacgccag
     1321 accatgtcaa ggaggcggcc aagcgagcga ttgatcagaa ccaatcctac tatacaggga
     1381 tgagtggtct gctgactcta cgtcaggcag ccagtgactt tgttaaggaa aagtaccaac
     1441 tggactatgc tcctgaaaat gaaattttgg ttacaattgg ggcgacagag gctttatctg
     1501 cgactttgac ggctattttg gaagagggag acaaggtact tttgccagct cctgcttatc
     1561 caggctatga accgattgtt aacttagttg gggcagaaat tgttgagatt gatacgactg
     1621 aaaatggttt tgtcttgact cctgagatgt tggagaaggc cattttggag cagggtgata
     1681 agctcaaggc ggttattctc aactatccag ccaatccgac aggaattacc tacagtcgag
     1741 agcagttaga ggccttggca gctgttttac gcaagtacga aatttttgtt gtctgtgatg
     1801 aggtttactc agaattgacc tacacaggcg aagcccatgt gtctctagga acgatgttga
     1861 gagaccaggc tattattatc aatggtttgt ctaaatcgca tgccatgaca ggttggcgtt
     1921 tggggctgat tttcgctcct gcggccttca cagcccagtt aatcaagagt caccaatact
     1981 tggtcactgc cgcaaatacc atggcgcaac atgctgcggt agaagccttg acggctggta
     2041 aaaatgatgc ggagcccatg aagaaggaat atatccaacg tcgggactat atcatcgaaa
     2101 aaatgactgc tcttggtttt gagattatca aaccagacgg tgccttctat atttttgcta
     2161 aaattccagc gggctacaat caagactcct ttgcttttct gaaggatttt gctcagaaga
     2221 aggccgttgc ctttatccct ggtgcagcct ttggacgtta cggggaaggc tacgtccgcc
     2281 tatcttatgc agccagcatg gagactatca aagaagccat gaaacgactt gaggagtaca
     2341 tgagagaagc atgattcagt ctatcacgag tcaaggcttg gtgctttaca atcgcaattt
     2401 tcgtgaggat gacaagctcg tcaaaatttt tacagagcag gttggcaaac gcatgttttt
     2461 tgtcaaacac gctggtcagt ctaagctggc gcctgttatt cagcccttgg tgctggcacg
     2521 atttctcttg cgaatcaatg atgacggact cagttacatc gaagactatc atgaggtcat
     2581 gacttttccc aagattaata gtgacctctt tgtcatggcc tatgcgacct atgtggcagc
     2641 tcttgcagat gctagtttgc aggacaatca gcaggatgct cccttgtttg cttttttgca
     2701 aaagactttg gagttgatgg aagcaggctt ggattatcag gttttgacca atatttttga
     2761 aattcaaatt ttgactcgat ttggaatcag cctcaatttt aatgagtgtg tcttctgcca
     2821 tcgggttggt caggcttttg acttttcttt caaatatgga gcctgcctct gtccagagca
     2881 ttatcatgag gataagagac gttgtcatct caatcccaat atcccctatc tgctcaatca
     2941 atttcaagct attgattttg agactttgga gaccatttcg ctcaagcctg gaatcaagca
     3001 agagctacgc caatttatgg atcaattata tgaagagtac gttgggattc acctaaaatc
     3061 aaagaaattt attgattccc tagcagactg gggacaatta ctaaaagagg aaaagaaatg
     3121 aaaaaaatcg cagtagatgc catggggggc gattacgcac ctcaggccat tgttgagggt
     3181 gtcaatcaag ccctatctga cttttcagat atcgaggttc aactttacgg agatgaagct
     3241 aaaatcaagc aatatctgac agcgacagag cgcgtcagca ttatccatac ggatgagaag
     3301 attgattcgg atgatgaacc tacgagagct attaggaata agaaaaatgc cagtatggta
     3361 ttggcagcca aggctgtcaa agatggtgaa gcagacgctg tcctttcggc tgggaataca
     3421 ggtgccttgt tggcagcagg attcttcatc gtgggtcgta tcaagaatat cgaccgtcct
     3481 ggactcatgt ctaccttgcc taccgttgat ggaaaaggtt ttgacatgct agaccttggt
     3541 gccaatgcag aaaatacagc ccagcacctc catcaatatg cggttctagg ttccttctat
     3601 gctaaaaatg tccgtggcat tgcgcaacca cgcgttggtt tgctcaacaa cggaacagag
     3661 agtagcaagg gcgacccgct tcgtaaggaa acttatgaat tactggcggc tgatgaaagt
     3721 ttgaacttta tcggaaacgt ggaagcgcgt gatttgatga atggcgttgc agatgttgtt
     3781 gtggcagatg gtttcacggg aaacgctgtg ctcaaatcca tcgaagggac agctatggga
     3841 atcatgggct tgctcaagac agctattaca ggtggtggtc ttcgagcgaa actaggtgcc
     3901 ctccttctca aggacagcct cagaggtttg aaaaaacagc tcaattattc agatgttggt
     3961 ggagcggtct tgtttggtgt taaggcacct gttgtcaaga ctcatggctc aagcgatgcc
     4021 aaggctgttt atagtacgat tcgtcagatc cgtaccatgc tagaaacaga cgtggttgcc
     4081 cagactgcgc gtgaattttc aggagaataa aagagatgac agaaaaagaa atttttgacc
     4141 gtattgtgac cattatccaa gagcgacagg gagaggactt tgtcgtgaca gaatccttga
     4201 gtctgaaaga cgatttggat gcggattctg ttgacttgat ggagtttatc ttgactctgg
     4261 aagatgaatt tagtatcgaa atcagcgatg aggaaattga ccaactccaa agtgtaggag
     4321 atgtggttaa aatcattcaa ggaaaatagc aatcggagtt ccaagtcaac ggaagtagat
     4381 ggtttttaga aat
//
LOCUS       DAVBCW010000125         4344 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVBCW010000125 DAVBCW010000000
VERSION     DAVBCW010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.125"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(10..657)
                     /locus_tag="VQ514_001860"
     CDS             complement(10..657)
                     /locus_tag="VQ514_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HET2500588.1"
                     /translation="MDFEYFYNREAERFNFLKVPEILVDREEFRGLSAEAIILYSILL
                     KQTGMSFKNNWIDKEGRVFIYFTVEEIMKRRNISKPTAIKTLDELDVKKGIGLIERVR
                     LGLGKPNIIYVKDFMSIFQVKENDLQKSKNLTSEVKDFNLRSKENELQEVKNLDSNYI
                     ENNKSKYSKREYSFGENGLGTFQNVFLAAEDISDLQIIMNSQLENYIRLPAKLES"
     gene            complement(766..1284)
                     /locus_tag="VQ514_001861"
     CDS             complement(766..1284)
                     /locus_tag="VQ514_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HET2500589.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            complement(1511..2380)
                     /gene="rsmI"
                     /locus_tag="VQ514_001862"
     CDS             complement(1511..2380)
                     /gene="rsmI"
                     /locus_tag="VQ514_001862"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HET2500590.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGTSAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFGS
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETPLKGECLLIVEGASQDVEEKDEEDLFLEIQARIQQGMKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            complement(2383..2700)
                     /gene="yabA"
                     /locus_tag="VQ514_001863"
     CDS             complement(2383..2700)
                     /gene="yabA"
                     /locus_tag="VQ514_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HET2500591.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            complement(2740..3630)
                     /locus_tag="VQ514_001864"
     CDS             complement(2740..3630)
                     /locus_tag="VQ514_001864"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HET2500592.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            complement(3627..4265)
                     /locus_tag="VQ514_001865"
     CDS             complement(3627..4265)
                     /locus_tag="VQ514_001865"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET2500593.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVKVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
BASE COUNT         1207 a          984 c          694 g         1459 t
ORIGIN      
        1 aatcatgaac taggattcta gttttgcagg aagtctaatg taattctcaa gctgtgagtt
       61 cattatgatt tgtaaatccg atatatcttc agcagctaaa aacacatttt gaaatgttcc
      121 aagtccgttt tcaccaaaac tatattctct cttactatac ttactcttat tattctctat
      181 atagttagag tcaaggttct taacctcttg aagttcattt tctttacttc tgaggttaaa
      241 atcttttact tctgaagtta agttttttga cttctgtaag tcattttctt ttacctgaaa
      301 tatactcata aagtctttaa cataaatgat gttcggctta ccaagtccaa gccttactct
      361 ttcgatcagt cctattcctt tttttacatc aagctcatct aatgttttta tggcagttgg
      421 ctttgagata tttcttcttt tcataatttc ttcgacagta aaatagataa atactctgcc
      481 ttccttgtct atccagttat tcttaaatga cattcctgtc tgtttaagaa gtatggaata
      541 aaggataatt gcttctgctg ataagccccg aaattcttct ctatcaacta atatctccgg
      601 tacttttaag aagttaaatc tttccgcttc tctgttataa aaatattcaa agtccatgca
      661 agatcacctc ccttcttaaa aatttgcaaa gaaaaagacg atagtttttc ttctatcgcc
      721 tttggtaacc atttttatga aattttttaa agttaaaaat ataatttata atactatctt
      781 cttccatttt ctgattttag ttctgaaagt tccaaaaggt gcaaccgtat ttacatgaat
      841 aaacttatac acttcccaag tcgctgtttt agtcgcttca tcagcccact ttctcatatg
      901 cggttcaaat aattcttcct cactcaacga atcaatcatt gcagagatag aattaatatt
      961 ttcatttaat tttgctttca actcttgcag agataaatga gcgtaggtat ctgtgaacca
     1021 ctgatataat tcaccaagtt gattccattt aaatttatcc gatggtgttt ttacttgaag
     1081 cccctttctt tcatcttctt cccatttaag aaccaagttg gtccaaccaa cctgataagc
     1141 aaggttttct gctggagttc tgtcaacttc atcagctctc ttatctttta aattttctgg
     1201 aatattatca aattctaaaa tatatttctc aaatgttttc tctatctcag cttttagttc
     1261 ttctttattt tcgtatgttc gcaaaaaatc accttctttc tgatttatca atcctttgat
     1321 ttaaaatgac cttatttttc tatttatacc ttctaagaac gctataactg ctgctccaaa
     1381 catcggtatt ccgtatgggc ttaataaaaa acttaatacc aaagcttcta taccaagtga
     1441 tacatcttta tattatcatc actaattaag ttaaacagaa acagacagaa ttattacatc
     1501 ctgtctccct ttattgtttt tcttcccaat cgtggtaggc agcgtagagt tgactcttat
     1561 tccactggta aatcttagct acttccttaa tagcttgatt tttcttcatg ccttgctgga
     1621 tacgggcttg gatttctaag aacaagtcct cctcgtcttt ttcctccaca tcctgactgg
     1681 caccttcaac aataagaagg cattcgccct tgagtggcgt ttcagcgatg ctttccagta
     1741 attcagaaat tgtacctcgt tggtattctt catagatttt ggtcaattcc ctgaccaaaa
     1801 caaccgagcg gtcaccgtag acttctaaca tattttccaa cgtgtctgct acacgatgag
     1861 gtgattcata aaaaatctgt gtttcaggat aatctttttt agagccaaaa aattgcttct
     1921 gttgacctga ttttctcggt aaaaaaccgt aaaagatatg tggctgtggc gctaaaccac
     1981 tggcaatcaa ggcagaaatt cctgcagagg tacctggaac agtcacaact gcaatttctt
     2041 cctcaatagc tgccttaact aaatcatgac cagggtctga aatgctaggc aaaccggcat
     2101 cagagacctg agcaatactt tgccctgctt tcaagaaacc aatcaaatca ggaatttttt
     2161 ccttggcatt gtgctcatga aaactgatct gcttggtgga aatgtcaaaa tgcttgagca
     2221 aaagccctgt attgcgcgta tcctcagcag caatccagtc cacttctttc aaggtctgga
     2281 tagcacgaaa agtcatatca tctagattgc caatcggcgt tgccactaga tacagcttgc
     2341 catagggaga ctgcccctta aaactttttt gaatttgcat gcttactccc tgtataacaa
     2401 ctcatcacag aacatacatt cttcgtcctg ctctcgacgt tgtccataaa aatcattaca
     2461 tacgtgaaat ccatcacggt aaatacgacg gacactttcg cgaacatgct tggccttgac
     2521 gggagcgtct gcttccacct cacccaagcg ttctcgcaac ttactatttt ccaagcgaag
     2581 agctgtattt tcttctacca agctcttgag atttttcttg atggcttcca catcggctaa
     2641 agttaccaat aattgttggg aaaaatcatc cagcgcgtca aataattctt ttttgtccat
     2701 aaaacagccc tttcctttct ttatcattca tttttgagtt tatatttctt tcaagaccag
     2761 atattccatg gcattttgaa agctgacatt agcttgccac atttttctag cttctagtaa
     2821 atcttgtaga atcactctta ctcttacctg caagaggtcc tgcccacaga gaacttcaag
     2881 aatccgtaaa acctgatcct gtttttcctt atcatctgcc aagttggcta atttggcaac
     2941 ctgtagataa ctttcttttt tcttagctac taaccaagtc agcaggcgtt cactttcatc
     3001 gaccaaggtc caaaaacttg cctgattagc caacttttct gcttcagctc gcgattgact
     3061 aaacttagct aaaagagtcg cttttttctt aacaagtccc atttgttcta agagtaagat
     3121 aagtttttct tcttgctttt taaagtggaa gatctgagtc cgacttcgga ttgtcggtaa
     3181 catcttttcc tcatcgctag tcaagaagaa aatataaact tcactctggg gttcttcgat
     3241 gaccttgagc agagaattgg ctgcgttggg atgcatttta tccgcttgct cgatgataaa
     3301 gacctgttgc tggctttcaa tccctgcttg agaaaactgt cccaccaatt cccgaatacg
     3361 ttctgtcttg atgacctgat tgactggctt aatcaaggtg acatctggaa actcttcctg
     3421 ttcaatcagc ttgcaacttc ggcatttctc acatggtaag acgccaactt tatccgtaca
     3481 aaagaggctc ttagctaaaa attgcgccat ttccaagctt ccaaagaaac ctgaaaagag
     3541 ataggcgtga ttgagctggt cttgttctaa gatacggaca aaacggtcaa actgagctgg
     3601 ttgccaagcc tttagttgat cttgtttcat ttggccaagc ccattccgtc aaacaagaca
     3661 gccttggtag tttccacaac ttgctccaaa gggagactag catcaatctt gacaatgcga
     3721 tttccctctt tatccagaag agaaaggtag ccttgacgaa cttttttatg caagtccaac
     3781 ccttccaaat ccaaacgatt aacctcgcgg tcactattag cagcaatacg agccagccct
     3841 tcttccacct cgatgtcaaa atagagtgtc aaatcgggtt tgaggccatc tgtcgcaaac
     3901 tgattgagcc agtcaatggc ttcaatatct aagccacgac caaatccctg ataggcaaca
     3961 gaactatcga taaaacgatc catgatgacc aacttgccag cttcaagggc tggaagaact
     4021 ttttccacca aatgctgtct gcgactggca atatagagaa gtagctctgt tttagcatcc
     4081 atctgagtat gacttggatc caaaatcact tcccgaatct tctccccaat caagactccg
     4141 ccaggttcac gggtcgtcag cacctttact cctttttcct ctaaaattgg tagcagagcc
     4201 tctaaaacac tggtcttgcc tgctccctct ggtccctcaa gagagactaa aaatcctttt
     4261 gacatgtcta actcatttct tttttactac ttctattcta tcaaaaaaat aggggtttgt
     4321 gacaatcttt ttgtcttctc attt
//
LOCUS       DAVBCW010000126         4227 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVBCW010000126 DAVBCW010000000
VERSION     DAVBCW010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.126"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            175..1341
                     /locus_tag="VQ514_001866"
     CDS             175..1341
                     /locus_tag="VQ514_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET2500594.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVANPAT
                     YLKKDVATVLVSFARSGNSPESLATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTTLLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            1457..2494
                     /locus_tag="VQ514_001867"
     CDS             1457..2494
                     /locus_tag="VQ514_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HET2500595.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHVYNGTLLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            2702..3556
                     /locus_tag="VQ514_001868"
     CDS             2702..3556
                     /locus_tag="VQ514_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET2500596.1"
                     /translation="MTYEYKSHIYLAETALNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREYYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLEDKFSVPSASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWMTSSQLEITDSDGI
                     VTRIRLAR"
     gene            3706..3963
                     /locus_tag="VQ514_001869"
     CDS             3706..3963
                     /locus_tag="VQ514_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HET2500597.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDRDCADIVTQLTAVRSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
BASE COUNT         1259 a          764 c          967 g         1237 t
ORIGIN      
        1 cggcaacctc aaagcagtgc tttgagcagc ctgcggctag tttcctacag attttagttg
       61 gaactcgatt caattcatgt gacaacgtga aaatcgttag agcattttat atagaatata
      121 catgggaatg tagcttactc ccattcccat atttaataga aaaagaggaa ctcaatgcta
      181 cattatacaa aagaagactt gctcgaattg ggtgcagaaa tcactacgcg tgaaatctac
      241 caacagcctg atgtatggag agaagctttt gaattttatc aagcaaaacg tgaagaaatt
      301 gcagccttcc tacaagaaat cgctgataaa catgactata ttaaggttat cttgacaggt
      361 gctgggactt ctgcttatgt gggagatacc ttgctacctt attttaagga agtctatgac
      421 gaacgcaaat ggaatttcaa tgctattgcg acaacagata tcgttgccaa tccagcaacc
      481 tatttgaaaa aagatgtggc aactgtcctt gtgtcttttg ctcgtagtgg gaattcgcct
      541 gaaagtttgg cgactgttga tttggccaaa tccttggtgg atgagcttta tcaagtgacg
      601 attacttgtg cagcagatgg taaattggct cttcaagctc acggtgatga tcgtaatctc
      661 ttgctcttgc aaccagctgt ctctaatgat gctggatttg ccatgacttc tagctttacg
      721 tctatgatgt tgacaactct cttggtcttt gatcctacag aatttgctgt taagtctgaa
      781 cgttttgaag ttgtatctag tcttgcccgt aaagttttag acaaggcaga agatgtcaaa
      841 gagctcgttg atttagactt taaccgtgtc atctatctag gcgctggtcc tttctttgga
      901 cttgctcatg aagctcagct caagattttg gaattaactg ctggtcaagt tgcgaccatg
      961 tatgaaagtc cagttggctt ccgtcacggt ccaaaatctc ttatcaacga caatacagtt
     1021 gttttggtct ttggtacaac gacagactac actcgtaagt acgacttgga cttggttcgt
     1081 gaagttgctg gtgaccagat tgctcgtcgt gttgtgcttt tgagtgatca agcttttggt
     1141 cttgaaaatg tcaaagaagt ggcccttggt tgtggcggtg tcttgaatga tatttaccgt
     1201 gtcttccctt acatcgttta tgcccaactc tttgctttat tgacttcact caaggtagaa
     1261 aataaaccag atacaccgtc tcctacaggt acagtaaacc gtgtagtaca aggtgtcata
     1321 attcacgaat atcaaaagta agacagtgtt tatgaattct tgacaagagg atttgtaaat
     1381 tatcagataa accatagatt gtcagtacgc tttctatggt ttgtttgctt gagagaaata
     1441 gtaaaaggag aacagaatga aagcatacac agagcgtgta tttggaaatg ttgagggtga
     1501 ggatgtcttg gcctatcgat ttgagacaga cggtggctac caacttgagg ttatgactta
     1561 tggtgcgact atcttgcgct atgtcgcacc tgacaaggct ggaaattttg ccaatgttat
     1621 cttgggattt gatgactttg atagttatgt aggcaatagt cccaagcatg gagcaagtgt
     1681 aggtcctgta gcgggtcgta ttgcaggtgc gacctttgag ctcaatggta agacctatga
     1741 ccttgaggtt aataatgcta gcaactgtaa tcacagtggt tcaactggtt gggattccag
     1801 cttgtttgaa gttgaagaag taagcgatca tggcttgact ctctacacag agcgtacaga
     1861 tgggacagga gggttccctg gaaatctcaa gatttggatc agttatcact tggaagaaac
     1921 tggtgcctat gaaatcagct acaaggtaac gaccgatcag gatacgctgg tcaatccaac
     1981 caaccacagc tatttcaact tgtctggtga tttcacgcag acgattgacc gtcatgtctt
     2041 ccaactaaac acagagggca tttactcaat cgctcctgac ggtgttcctg ccaaaactcc
     2101 agaagccaac cgtgatgtgg tcaaacacgt ctacaatggt accttgttga aggatatctt
     2161 tgcagaagaa gatgagcaaa tccagctggc atcaggtttg gatcatccat ttgcccttcc
     2221 tgcaggccat gacaatgctg gattccttta tgaccaaaat tcaggtcgct tcctgctttt
     2281 caagacagaa gctccttgct ttgtggtcta cacagcaaac tttgtggatg aaagtgtcat
     2341 cataggaggt cagccaatgc tacagcacaa tgggattgct cttgaagcgc aagctttacc
     2401 agatgccatt cacagtgacc ttaaaggcca agtcattctt aaagctggtc aaaccttcac
     2461 cagtaagaca cgttatgaac ttgttgtgaa ataaaaaaag ctggtcaaaa agtctttaaa
     2521 attaaaataa cgcatattgt caggtgatga aaagtttgat aatatgcgtt ttattatgaa
     2581 atgatttact ttatattatc ctgaaatggt gttatttttg agttatctgg cttgactttc
     2641 ttgatggaaa ttatataata gttgtaacaa tgataattac aactagaaaa gaggaccatt
     2701 tatgacctat gaatacaaga gtcacattta tttggcagag acagctttaa atgtaaagga
     2761 tttggccagt caaacagcct tttatcagca agttattggt ttagaaatct tatctcaaac
     2821 tgagacagag tccattctag gccttggtgg aaaagtcttg gtacagctga ttcaagcaca
     2881 agagagcgga gaagtgaggg aatattatgg tctttaccat ttggccattc tcttgcccac
     2941 acgcaaggct ttagctgatg tcttgaaaca cctgacagat ttacaaattc ctcttgtcgg
     3001 cggtgcagac catggttaca gtgaagctat ttacttggaa gatttggagg gaaatggcat
     3061 tgaactctat cgagataagc cagtttccac atgggatatt cgagaagatg gacgcattat
     3121 cggagtgaca gaggctcttg cggcgcagga catttatgag ttgggggaaa gagtagagcc
     3181 ctttatcctg gcagaaggta cgagaatggg gcatattcat ctttccgtca aggatagtcg
     3241 aaagtccagc cagttttatc aaaaggtgtt agggctagag gataaattca gtgtgcctag
     3301 cgctagttgg attgcggctg gagattacca tcatcattta gcagttaacg aatggggagg
     3361 aaaaggtctg gcttcgcgta agcaaggctt accaggttta gcctactatg tcatcgaagt
     3421 cgcacacaaa aaagaactat taacgattgc ccaacgagca caagaagttg acgcaccaat
     3481 caaatggatg acatcgagcc aattggaaat cacagactca gatggcatcg tgacccgtat
     3541 tcgtttagca aggtaaaatt aggaattttt aataagttat aagtattgct agtttgcttt
     3601 ttgaacggtc tttggtaaca gaactgcgtg agctggatgg gcgagaattt tgacaaagaa
     3661 gctgattaga gatataatga agaaaaatcg agcaaggaaa aagaaatgac aaattcaaag
     3721 tatattactt gtttgaaacg ttcagagggc cagttgtgtg ggattcaaaa gatgattgaa
     3781 ggagatcgtg actgtgctga tattgtgacg caactgacag cagtgagatc tagtgtagag
     3841 cgcgtgattg agatgataat taccgaaaat cttactgaat gtatcaatca gccactagat
     3901 gattctgaag ctcaaaagga acgcttagaa aaggctatcc gatacttgat taaacggaaa
     3961 taaatgaatg ttttgagacg atatgtgcaa aggtctatta actattagaa tagtttaatg
     4021 ggagtcaaaa gaagttgtaa aaaatcttta aaattaaaaa aacgcatagt atcaggtatt
     4081 gaaatgtact gaccccaaaa agttagattt tttctgtcta acttttgggg tgcagttcat
     4141 aagaaccttg gtaatatgcg ttttattatg gggagattta tacttttcga aaatctcttc
     4201 aaaccaggtc agcttcacct tgccgta
//
LOCUS       DAVBCW010000127         4194 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVBCW010000127 DAVBCW010000000
VERSION     DAVBCW010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.127"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(<1..1275)
                     /locus_tag="VQ514_001870"
     CDS             complement(<1..1275)
                     /locus_tag="VQ514_001870"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HET2500598.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIVMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQAHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVALPIENALDVYIA
                     VLGDGANVKALELVQTLRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLG"
     gene            complement(1353..2021)
                     /locus_tag="VQ514_001871"
     CDS             complement(1353..2021)
                     /locus_tag="VQ514_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2500599.1"
                     /translation="MSLSMSSADGENLKNEKLSIKDQFLTIYRNLRLVFLKSGQKNFG
                     FMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALFINQFCVLVAVIISSLTGND
                     YFGKQSLPRLMMWATVGLSLVGLANLFNQVVLGLLFLFFTLYVSGKVQPKISAMLMKN
                     LAPEVLARTSNFLGLLFTLSIPVGTACFSLVAVWNMQLTWMLFVGLSLLAIFLTILNL
                     KNDI"
     gene            complement(2333..2599)
                     /locus_tag="VQ514_001872"
     CDS             complement(2333..2599)
                     /locus_tag="VQ514_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500600.1"
                     /translation="MKLLFRNQAYRLLTLSRFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTFFTVFAGIRADYTRDKVKWMVYSGLFQAVLFF"
     gene            complement(2789..3640)
                     /gene="rgg"
                     /locus_tag="VQ514_001873"
     CDS             complement(2789..3640)
                     /gene="rgg"
                     /locus_tag="VQ514_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HET2500601.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELLV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSYNKQKHILNAIIIKSYMKSIDERVELTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNFFHTILLNGFLASIECEKFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            3764..4105
                     /locus_tag="VQ514_001874"
     CDS             3764..4105
                     /locus_tag="VQ514_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HET2500602.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIEDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
BASE COUNT         1331 a          883 c          820 g         1160 t
ORIGIN      
        1 tcctagtttt tcaaagattt ctgagaagtt ttggctgatt gtctcaagtg acacttgcac
       61 ttcttctcgg gtttggttgt tcttgaccgt cacttgtccg ctttcgactt cgctctctcc
      121 tagggtgatg agggtcttag ccgcaaagac atctgctgac ttgaactgag ctttgagctt
      181 acggttgagg taatcacgct ctgctttgaa accttgttgg cgaagagttt ggactagttc
      241 tagggctttg acatttgctc catcacccaa gactgcgata tagacatcta gggcgttttc
      301 gatagggagg gccacgcctt gtttttcaag gatgagaagc aggcgctcta caccaagccc
      361 aaaaccaaat ccagcagttt cagggcctcc aaagtaagca accaaaccat cgtagcgacc
      421 acccgcacag actgtcaagt cattgccctc aatctctgtg ataaactcga aaatggtgtg
      481 gttgtagtag tccagaccac gcaccatatt ggtatcgatg atgtaatcta ctccaagatt
      541 ttccaacatc tgacgcacag catcaaaatg agcttggctt tcttcatcaa gaaagtccaa
      601 gatagacggc gcattctcta ctgccacctt gtcttctttt tccttagagt ccaagacacg
      661 aagaggattt tcctccaagc gacgttggct atccttagac aaggtctcct tgagcggtgt
      721 caaatagtca atcaaggctt ggcggtaggc tgcacggctc tcaggatttc caagagtgtt
      781 gaggtgcaat ttgacacctt gaataccgat ttccttcaaa aaatgggctg ccataacgat
      841 tgtttccaca tcggtagctg gattgctaga gccaaaacac tcaacaccaa tctggtggaa
      901 ttggcgcaag cgccctgcct gtggacgctc ataacggaac ataggtccca tgtagtagaa
      961 cttgcttggc ttttgcactt ctggggcgaa gagtttattt tccacatagg aacggacaac
     1021 gggcgcagtt ccttctggac ggagggtaat atgacggtca cccttgtcat aaaaatcgta
     1081 catttccttg gttacgatat ccgttgtatc tccgacagag cgactgataa cctcgtaatg
     1141 ctcaaaaata ggcgtgcgca cttctgcgta gttgtagcgt ttgaaaattt cacgggcaaa
     1201 gccctcaacg tactgccact tggcagattc agcaggtaaa atatcctgcg ttccttttgg
     1261 tttttgtaat ttcataggga atcctcttta aacttaatag tcttatttta ccataaatag
     1321 agggattaaa acagtgagaa aaaaattagg atttagatat catttttgag attaagaatt
     1381 gttaaaaaaa tagctagcaa ggaaagacca acaaatagca tccaagtcaa ctgcatgttc
     1441 catacggcta ctagtgaaaa acaagctgtt cccacaggta tggataaggt aaacaataga
     1501 cctaaaaaat tactagtacg agctagaacc tctggagcta gatttttcat gagcatggca
     1561 ctaatctttg gttgaacttt accagacaca tacagagtaa agaagagaaa tagcaaacca
     1621 agcacgactt gattgaataa attagccaaa ccaactagac taagtcctac ggtcgcccac
     1681 atcatcaatc taggcaagga ctgcttccca aaataatcat tgcccgtaag gctactgatg
     1741 atgactgcta ctaaaacaca gaattgattg ataaatagtg cctctgtata agaaaaattc
     1801 aagagagaat ggctcaaaaa gaagatatta taaattccac ccaaagcgcc acccaaggaa
     1861 ttaataagca agacagcaaa gagcataaaa ccaaagtttt tctgtccact tttaagaaaa
     1921 acgagacgta aatttcggta aattgttagg aactggtctt tgatagaaag cttctcattt
     1981 tttaagtttt caccatcagc agatgacatt gacaggctca atttgctttt tcctaaaaag
     2041 aggatagtgg ctgatacgag gaaaaagcaa gcattaattc ccgcaacgag ggaaaaattg
     2101 ttgaccgata gagctaagag ccagactccg aaagcttgac caccaatagc tgaaatatag
     2161 gtgatgaact gtgaaaaaga ataagcctcc atcagatcat cttcagctac tttttcctta
     2221 ataagaggca tacgcaggcc acctgcaaaa tcactgatga tatcactaat gacattgatc
     2281 aaacacaggc tagaaaaggc aaagagacta gcttgctgaa caactagggc tgctagaaaa
     2341 atagaaccgc ctgaaacaaa ccgctataga ccatccattt gaccttgtcc ctcgtgtaat
     2401 ctgcccgaat ccctgcaaaa actgtaaaga aggtcggaag aatcatgaca atattcgcca
     2461 tagcaacagc aaaagatgct tgtgacaagg tcgatgcata gacgataaag accaggttga
     2521 aaatcgaagc accaaaagca ttgaagaagc gtgacaaagt caataggcga taagcttgat
     2581 ttctaaacaa tagtttcata gataatctcc ctccttgttc taagttctac atcaagaata
     2641 gctttagtat agcacttatg aatttgcgaa acgattcttt tgaatatatg caacaaataa
     2701 aatgagcaaa aaacgataga acttttgtaa tgacctgaga aagggcaaca gttctatcgc
     2761 ttcaaatttt ttcttagttt gcagatattc aacattcggt cgtatttcta tagtattcgg
     2821 cagatttatt acagccaagc atctcaaaaa tacggacagc atcctccatc tttttctggc
     2881 cttccttgac tctaccttgc ttgctatcaa ggagaccttc tgcccacaga taaacaattc
     2941 ggaaataggt ctcattttcc ttgtagaaat gctcttcgat aacacgttta aaataatagg
     3001 cattggtaaa tttttcacac tcaatactag ctaaaaagcc attcaatagt atagtatgaa
     3061 aaaagtttcg attaccagac atttccatta gaaaatcaga tttacgtacc atttctcgta
     3121 catatctagt aaaaagagaa acagataaaa atggagaact gactgaaaat aaattgagtt
     3181 catagattcc ccagatctcg gtagaaaaca aataatcatg aaggactttt ccttcctctg
     3241 ctgttaactc taccctttca tctatgctct tcatataaga cttgataata atggcattta
     3301 gaatatgttt ctgtttgttg taagaatggg catgcttttg atactccctg cgatataagt
     3361 cctcaagagg tgctatattc tttggttcca agacatctgt aatttctttt ctcaactcag
     3421 aatctgtatc atactggaaa cctctcgcca gaaagaggat ctcctccaca ctggcagata
     3481 tattttccag agcaaataga aacttttcca ccaaaagctc actctgacct gtttcaaaac
     3541 gggacaacat agacggcgaa aattgtcctc cggttgcttg tctcagtgag atatttcttg
     3601 actctcgtaa ttgtctaaag acttttccaa tctgctccat agacttcccc ttgattccgt
     3661 attttcttca ttttatcata tttttcagaa aattcatcaa aaacttgcca aattgtcaga
     3721 attatgagaa aatagaggat atttatcacg tggagggact gctatgagag acgatatcaa
     3781 aatcaatgac cgtgctttgg ccttgcaaga ccaaattatc gaaaaactag agaaagtttt
     3841 tgatacagat gtggaattgg atgtttacaa tctaggtctg atttatgaaa tcaatctgga
     3901 tgaaacgggg ctctgcaaga ttgtcatgac cttcaccgat actgcctgtg attgcgccga
     3961 aagcctgcct attgaaatcg tggcaggtct gaaacaaatc gagggtatcg aagatatcaa
     4021 ggttgaagtt acctggtcgc ctgcttggaa aatcacacga atcagtcgct atggccgtat
     4081 tgcccttgga ctaccacctc gttaagcagg ccaatcactt ttaaagatga aaaaagcaag
     4141 ccgaaaatgg cttgcttttt gatactcaat gaaaatcaaa gggcaaacta gaaa
//
LOCUS       DAVBCW010000128         4178 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVBCW010000128 DAVBCW010000000
VERSION     DAVBCW010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.128"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(26..199)
                     /locus_tag="VQ514_001875"
     CDS             complement(26..199)
                     /locus_tag="VQ514_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HET2500603.1"
                     /translation="MNIKKRVLSAGLTFASVLLLAACGQSGSDTKTYSSTFSGNPTTF
                     NYLLDYYTDNIVN"
     gene            complement(357..1964)
                     /locus_tag="VQ514_001876"
     CDS             complement(357..1964)
                     /locus_tag="VQ514_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HET2500604.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGIGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIAAIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENPNSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLVRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVELL"
     gene            2117..3601
                     /locus_tag="VQ514_001877"
     CDS             2117..3601
                     /locus_tag="VQ514_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HET2500605.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFLNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGISPADRKVAVVARQKAEETGGPALALELPNGDIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            3696..4178
                     /locus_tag="VQ514_001878"
     CDS             3696..4178
                     /locus_tag="VQ514_001878"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HET2500606.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
BASE COUNT         1207 a          957 c          799 g         1215 t
ORIGIN      
        1 gaggctcttt tgtcttgttc ttgtttcaat tgactatatt atcagtgtaa tagtctaata
       61 gatagttaaa tgtagttgga tttccactaa aggttgatga gtaagttttt gtatctgaac
      121 ctgattggcc gcaagcagct aaaagcaaaa cagatgcaaa agtcaggcct gcactaagga
      181 cacgcttctt tatgttcatc ttctttctcc ttaatagtgg gaatttgtaa agttaattga
      241 atttcgagaa tgaaagttta taaaccttgg ttataaaaaa caaaggattt ctgcttttat
      301 acagtcctcc cttgttttta tatgatttca attttaaatt tttctgcaaa aaatatttat
      361 agtaattcca cacagaaagc atcccatgga actaagattt gtttttcaaa gacttcttga
      421 gctagggtgt tttcaatcaa gacagatttg acttttcctt ctactgtcaa gtcttgctct
      481 tcattggaca agttagccac aactaggaag cgacggtcgc catccttacg tatataagca
      541 aagaccttat cagccgtatc aagcaattca aagtcagctc gaactagcca gctattctcc
      601 ttgcgaattt ggaccagttt ctgataggta tagaaaatag aatctggatt tgccagcgct
      661 tcttggacat tgatcatctc gtaatttgga ttaaccgcca accaaggttg acctgttgag
      721 aaaccagcgt ttttgctctc gtcccattgc ataggggtac gggcattgtc acgtccaata
      781 acacggatac tgtccatgat ttcttcaatc ggaacacctt tttcaagagc ctcacgcgca
      841 tagttgagag attcaatatc ttctacttga tccagtgttt caaacggata gttggtcatc
      901 ccaatctcct caccttggta gatataagga gttcctctca taagatgaag caagattgca
      961 aaggctttgg cagatttttc gcggtattct tggtcatttc cccagattga gacaatacga
     1021 gggaggtcat ggttgttcca gaagagggaa ttccagccgt cctcaactcc taactctgtc
     1081 tgccatttgt tgaagatttc ttttaactta gcgatattca gctctttttg atagtgccat
     1141 ttaggctgac cttcctgata ctgaagaccg atatgttcaa actggaagac catagacaat
     1201 tcttgcccct ttggatcaga gtagagcttg gcaatctctg gcgttgctcc ccaagtctcc
     1261 cctactgtca agagatcctt atctccaaag gtcgcctgat tcatttcctt gagataggga
     1321 tggagcatag gaccattatt gactaccttc tcgtcaggaa ttttgccaat catgtcaata
     1381 acatccatac ggaaaccacc aataccctta tcaatccaga agttcatcat ctcataaatt
     1441 ttctggcgaa gtttttcatt ttcccagttg agatccggct gtttcttgct gaaaaagtgg
     1501 agatagtatt gacctgactt ttcatcgtat tcccaagcag acccactaaa gatagaatct
     1561 aaatcgttag gctcatcccg ccagatatag tagtctcgct cagggctatt aggattttca
     1621 caggcctcta caaaccaggc atgctcatcc gaggtatgat tgaccaccaa gtccatgatg
     1681 atacggatat cacgcttctt agcttctgca atcagctgat ccatgtcctc catggttcca
     1741 aaaatagccg caatcgcttg ataatcagca atatcgtagc cattatcatc catagggctg
     1801 tcataaacgg gagaaagcca aattgctgtg attcctagct tggctagata gtccaactta
     1861 ctggtaattc ctggcaaatc gccaattcca tctccattac tatccataaa actctttgga
     1921 tagacttgat agactacggc attatgccac catttttctt gcatctgttt acctcattat
     1981 tttcataatc tatagtctat gatagcactt tttaaaattt tgtgcaagcg tttgcttaat
     2041 ctttttaatt tcttttttag ctccgtagtt tcctaacttc caatttttta ttataataga
     2101 gtccagaggt aaaaaaatga aaaaacaagc ttttagttct gaacagtatt tgaatttaca
     2161 acgcgaccat attttggagc gcattaacca atttgacggc aagctctact tagagtttgg
     2221 cggtaaaatg ttagaagatt tccacgctgc tcgtgtcctt cctggttatg aacctgacaa
     2281 caaaatcaag ctcttgcaag aattgaaaga gcaggttgag gttgtgattg ccattaatgc
     2341 tagcaacatt gaacattcca aagcacgtgg cgacttgggc atttcttatg accaagaagt
     2401 tcttcgtttg attgacaaat tcaatgaatt agggattttt gttggttccg ttgtcattac
     2461 acaatacgct ggccaacccg ctgcagatgc cttcctcaac caacttgaga aaaacggaat
     2521 tgattcttat cttcattatc caatcaaagg atatccgacg gatatggatc acatcatttc
     2581 cccagaaggc atgggcaaaa acgactacat caaaaccagt cgcaacttga ttgtcgtaac
     2641 cgctcctgga cccggttctg gaaaattggc aacgtgtatg tccaatatgt accacgacca
     2701 aatcaatggc atcaagtctg gctacgctaa atttgaaacc ttccctgttt ggaatcttcc
     2761 ccttcatcat ccagttaact tggcttatga agctgccaca gctgaccttg atgatgtcaa
     2821 catgattgac cccttccatc ttcaaaccta tggagaaacc actgtcaact acaaccgtga
     2881 tatcgaaatc ttcccagtgc tcaagcgcat gttggaacgt attctcggaa aatcaccata
     2941 cgcttcaccg acagatatgg gtgtcaacat ggttggtttc gctattacag atgacgaggc
     3001 tgctgtcgaa gcttctaaac aagaaatcat ccgccgttac tatcaaacag ttcttgactt
     3061 caaagctgaa aaagttggcg aagctgccgt caagaaaatt gagttgctca tgaacgacct
     3121 cggtatctca cctgcagacc gtaaggttgc tgtcgttgcg cgccaaaaag cagaagaaac
     3181 tggcggacca gccctagctc ttgaattacc aaatggtgac attgtcactg gtaaaaactc
     3241 agaactcttt ggccctacag ccgctgcctt gatcaacgcc atcaaaaaat cagctgacat
     3301 cgctaaagaa gtaaaactaa tcgagcctga agttgttaag ccaatccaag gacttaaaat
     3361 cgatcatctc ggtagccgca atccacgcct tcattcaaat gaaatcctga ttgcacttgc
     3421 tatcacagct acagaaaatc ctgatgctgc ccgcgctatg gaagaactcg gcaacctcaa
     3481 aggaagcgaa gcccactcaa ccatcatctt gactgatgaa gacaagaatg tccttcgtaa
     3541 actgggtatc aacgtaacct ttgacccata ctaccaatac gaccgcttat atcgtaagta
     3601 aagatttcaa cctctccagc ctcggagagg ttttctctct gtctcaggag caagctttat
     3661 gatataatga gagaagaaaa ctgaaaggat ttaccatgtc aaaagaagtt attgtcgaaa
     3721 gttttgaact tgaccacacc attgtcaaag caccctatgt tcgcttgatt ggggaagaaa
     3781 caggaccaaa aggagacatc atctccaatt atgatattcg cttggtgcaa ccaaacgaag
     3841 actctatccc tactgccggc cttcacacta tcgagcacct cttagccaaa ctcatccgta
     3901 cccgcattga cggcatgatt gactgttcac catttggttg ccgcacaggc ttccacatga
     3961 ttatgtgggg acgtcacacc agtgctaaaa tcgcagctgt tatcaaggat tcgctcaagg
     4021 aaatcgctga aactactact tgggaagatg tcccagggac aaccatcgaa tcttgcggaa
     4081 actacaagga ccacagcctc ttctctgcta aagaatgggc aaaactcatc ttggaacaag
     4141 ggatttcaga tgatgccttt gaacgtcatg tgatttaa
//
LOCUS       DAVBCW010000129         4176 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVBCW010000129 DAVBCW010000000
VERSION     DAVBCW010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.129"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            343..570
                     /locus_tag="VQ514_001879"
     CDS             343..570
                     /locus_tag="VQ514_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500607.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            858..3755
                     /gene="nanA"
                     /locus_tag="VQ514_001880"
     CDS             858..3755
                     /gene="nanA"
                     /locus_tag="VQ514_001880"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HET2500608.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGESSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYNNYNDAPLKVKPGQWNSVTFTVEKPTAE
                     LPNGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESGRNGKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGNRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKKIDGKTYQILY
                     REGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGNQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NRRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHNGKEYIILSNAGGPNRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWEFLSKDLISPTEAKVKRTREMGKGEMG
                     KGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKG
                     SIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLV
                     TLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
BASE COUNT         1432 a          675 c          901 g         1168 t
ORIGIN      
        1 tgttttgagc atcatgcggc tagcttctta gtttgctctt tgattttcat tgagtataaa
       61 aacagatgag tttctgtttt ctttttatgg actataaatg ttcagctgaa actactttca
      121 aggacattat tatataaaag aattttttga aactaaaatc tactatatta cactatattg
      181 aaagcgtttt aaaaatgagg tataataaat ttactaacgc ttataaaaag tgatagaatc
      241 tatctttatg tatatttaaa gatagattgc tgtaaaaata gtagtagcta tgcgaaataa
      301 cagatagaga gaagggattg aagcttagaa aaggggaata atatgatatt taaggcattc
      361 aagacaaaaa agcagagaaa aagacaagtt gaactacttt tgacagtttt tttcgacagt
      421 tttctgattg atttatttct tcacttattt gggattgtcc cctttaagct ggataagatt
      481 ctgattgtga gcttgattat atttcccatt atttctacaa gtatttatgc ttatgaaaag
      541 ctatttgaaa aagtgttcga taaggattga gcaggaagta tggtataaat agcataagct
      601 gatgtccatc atttgcttat aaagagatat tttagtttaa ttgcagcggt gtcctggtag
      661 ataaactaga ttggcaggag tctgattgga gaaaggagag gggaaaattg gcaccaattt
      721 gagatagttt gtttagttca tttttgtcat ttaaatgaac tgtagtaaaa gaaagttaat
      781 aaaagacaaa ctaagtgcat tttctggagt aaatgtctta tttcagaaat cgggatatag
      841 atatagagag gatcagtatg aatcggagtg ttcaagaacg taagtgtcgt tatagcatta
      901 ggaaactatc ggtaggagcg gtttctatga ttgtaggagc agtggtattt ggaacgtctc
      961 ctgttttagc tcaagaaggg gcaagtgagc aacctctggc aaatgaaact caactttcgg
     1021 gggagagctc aaccctaact gatacagaaa agagccagcc ttcttcagag actgaacttt
     1081 ctggcaataa gcaagaacaa gaaaggaaag ataagcaaga agaaaaaatt ccaagagatt
     1141 actatgcacg agatttggaa aatgtcgaaa cagtgataga aaaagaagat gttgaaacca
     1201 atgcttcaaa tggtcagaga gttgatttat caagtgaact agataaacta aagaaacttg
     1261 aaaacgcaac agttcacatg gagtttaagc cagatgccaa ggctccagca ttctataatc
     1321 tcttttctgt gtcaagtgct actaaaaaag atgagtactt cactatggca gtttacaata
     1381 atactgctac tctagagggg cgtggttcgg atgggaaaca gttttacaat aattacaacg
     1441 atgcaccctt aaaagttaaa ccaggtcagt ggaattctgt gactttcaca gttgaaaaac
     1501 cgacagcaga attgccaaat ggtcgagttc gcctgtatgt taatggtgtt ttatctcgaa
     1561 caagtctgaa atctggcaat ttcattaaag atatgccaga tgtaacgcat gtgcaaatcg
     1621 gagcaaccaa gcgtgccaac aatacggttt gggggtcaaa tctacagatt cggaatctca
     1681 ctgtgtataa tcgtgcttta acaccagaag aggtacaaaa acgtagtcaa ctttttaaac
     1741 gctcagattt agaaaaaaaa ctacctgaag gagcggcttt aacagagaaa acggacatat
     1801 tcgaaagcgg gcgtaacggt aaaccaaata aagatggaat caagagttat cgtattccag
     1861 cacttctcaa gacagataaa ggaactttga tcgcaggtgc agatgaacgc cgtctccatt
     1921 cgagtgactg gggtgatatc ggtatggtca tcagacgtag tgaagataat ggtaaaactt
     1981 ggggtaaccg agtaaccatt accaacttac gtgacaatcc aaaagcttct gacccatcga
     2041 tcggttcacc agtgaatatc gatatggtgt tggttcaaga tcctgaaacc aaacgaatct
     2101 tttctatcta tgacatgttc ccagaaggga agggaatctt tggaatgtct tcacaaaaag
     2161 aagaagccta caaaaaaatc gatggaaaaa cctatcaaat cctctatcgt gaaggagaaa
     2221 agggagctta taccattcga gaaaatggta ctgtctatac accagatggt aaggcgacag
     2281 actatcgcgt tgttgtagat cctgttaaac cagcctatag cgacaagggt gatctataca
     2341 agggtaacca attactaggc aatatctact tcacaacaaa caaaacttct ccatttagaa
     2401 ttgccaagga tagctatcta tggatgtcct acagtgatga cgacgggaag acatggtcag
     2461 cgcctcaaga tattactccg atggtcaaag ccgattggat gaaattcttg ggtgtaggtc
     2521 ctggaacagg aattgtactt cggaatgggc ctcacaaggg acggattttg ataccggttt
     2581 atacgactaa taatgtatct cacttaaatg gctcgcaatc ttctcgtgtc atctattcag
     2641 atgatcatgg aaaaacttgg catgctggag aagcggtcaa cgataaccgt caggtagacg
     2701 gtcaaaagat ccactcttct acgatgaaca atagacgtgc gcaaaataca gaatcaacgg
     2761 tggtacaact aaacaatgga gatgttaaac tctttatgcg tggtttgact ggagatcttc
     2821 aggttgctac aagtaaagac ggaggagtga cttgggagaa ggatatcaaa cgttatccac
     2881 aggttaaaga tgtctatgtt caaatgtctg ctatccatac gatgcataat ggaaaagaat
     2941 acattatcct cagcaatgca ggtggaccga atcgtgaaaa tgggatggtc cacttggcac
     3001 gtgtcgaaga aaatggtgag ttgacttggc tcaaacacaa tccaattcaa aaaggagagt
     3061 ttgcctataa ttcgctccaa gaattaggaa atggggagta tggcatcttg tatgaacata
     3121 ctgaaaaagg acaaaatgcc tataccctat catttagaaa atttaattgg gaatttttga
     3181 gcaaagatct gatttctcct accgaagcga aagtgaagcg aactagagag atgggcaaag
     3241 gagagatggg caaaggagtt attggcttgg agtttgactc agaagtattg gtcaacaagg
     3301 ctccaaccct tcaattggca aatggtaaaa cagcgacttt cctaacccag tatgatagca
     3361 aaaccttgtt gtttgcagta gataaggaag atatcggaca ggaaattatt ggtatagcta
     3421 aaggaagcat cgaaagtatg cataatcttc ctgtaaatct agcaggtgcc agagttcctg
     3481 gcggagtaaa tggtagcaaa gcagcggtgc atgaagttcc agaatttaca gggggagtta
     3541 atggtacaga gccagctgtt catgaaatcg cagagtataa gggatctgat tcgcttgtaa
     3601 ctcttactac aaaagaagat tatacttaca aagctcctct tgctcagcag gcacttcctg
     3661 aaacaggaaa caaggagagt gacctcctag cttcactagg actaacagct ttcttccttg
     3721 gtctgtttac gctagggaaa aagagagaac aataagagaa gaattctaaa catttgattt
     3781 tgtaaaaata gaaggagata gcaggttttc aagcctgcta tctttttttg atgacattca
     3841 ggctgatacg aaatcataag aggtctgaaa ctactttcag agtagtctgt tctataaaat
     3901 atagtagatt gaaataagat gtgaacaact ctatcaggaa agtcaaatta atttatagaa
     3961 aaattttagc agtcaaggtg tactgttata gattcaatat attatgtgac tattaacctt
     4021 ctcttctcct aaaattgact ttcttgtttt cttatcttgt ccactcgaaa caagtattgt
     4081 aagaatttga ttatttttga aagtactttt aatatacttg atatagttaa aaaagatttg
     4141 aaactaaatt ccaaattaga aaaagacttg aaatac
//
LOCUS       DAVBCW010000130         4137 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVBCW010000130 DAVBCW010000000
VERSION     DAVBCW010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.130"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            720..1712
                     /locus_tag="VQ514_001881"
     CDS             720..1712
                     /locus_tag="VQ514_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2500609.1"
                     /translation="MAMIEVEHLQKNFVKTVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPDSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYDGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            1714..2532
                     /locus_tag="VQ514_001882"
     CDS             1714..2532
                     /locus_tag="VQ514_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HET2500610.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVIYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            2534..3319
                     /locus_tag="VQ514_001883"
     CDS             2534..3319
                     /locus_tag="VQ514_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2500611.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            3358..3636
                     /locus_tag="VQ514_001884"
     CDS             3358..3636
                     /locus_tag="VQ514_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HET2500612.1"
                     /translation="MFVIEEVKDENQKKAVVAEVLKDLPEWFGIPESTQAYIEGTTTL
                     QVWTAYQESDLTRFVSLSYSSEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            3621..3818
                     /locus_tag="VQ514_001885"
     CDS             3621..3818
                     /locus_tag="VQ514_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2500613.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFPQLWNPQNPCQILIKKLE"
BASE COUNT         1152 a          705 c          897 g         1383 t
ORIGIN      
        1 gctatatatt atggtatcga atcttcatca gaatgataaa attaatcaat tgatatctga
       61 ttacaaacag aatatgaaag ctttttatat cactattgaa aaatttatac gagatgatga
      121 aagccttaag tgttatttta taaaggttat ttcaagtcgt tccaaggtaa caagtctaga
      181 tcagattgaa gctgataaaa cgatacaaag aaaatattca agtgagctaa aaaaatttat
      241 tggattttat aatgagatta tttgtgagga aaatagtttc ctacatgtac gaaagaggtg
      301 gtcgagttgg tttaggtagt cgatgcgtga gttgataatt ctcagggtat ggacttcttt
      361 ttcatgaatg aggtaaaaga gcaggtattg tttagagaca atcattctga gcatattttc
      421 tggatagagg gagtatccga ttttatgatc aaagttaata ccgccctctg gtgagaagat
      481 gagtaggttg gtaatttaaa ctattaaaca gaatttttga ttaaaagtat tattttatga
      541 gagaaatcct aatttcacaa tccataggca aactcttgca tttcgttttt tattggacta
      601 taataggttg gtataaagcc ttttgtagta ataaaatgta gaaggtgtag aaagcaagga
      661 tttagaatat ttgtagttaa aaacacaatg ttgctattcc ttacgatagg gagatagata
      721 tggcaatgat agaagtggaa catcttcaga aaaattttgt gaagactgtt aaggaaccgg
      781 gcttgaaggg ggctttgcgc tcctttattc atcctgaaaa gcagaccttt gaagcggtca
      841 aggatttgac ctttgaggtt ccaaaagggc agattttagg atttatcggg gcaaatggtg
      901 ctgggaagtc gacaaccatt aaaatgctga caggaatttt gaaaccaaca tctggttttt
      961 gtcggattaa cggcaagatt ccccaggaca atcggcaaga ttatgtcaaa gatattggcg
     1021 tagtctttgg acaacgcacc cagctatggt gggatttggc tctgcaagag acctacactg
     1081 tcttaaaaga gatttatgat gtgccagact cgctctttca taagcgtatg gactttttga
     1141 atgaagtctt ggatttgaag gactttatca aggatcccgt gcggactctt tcactgggac
     1201 aacggatgcg ggcggatatt gcggcctcct tgctccacaa tcccaaggtt ctttttttag
     1261 atgagccgac cattggtttg gacgtttcgg ttaaggataa tattcgtcgg gcaattactc
     1321 agatcaatca agaggaagaa actaccattc ttttgaccac tcacgatttg agtgatattg
     1381 agcaactttg tgatcggatt ttcatgattg acaaggggca agagattttt gatggaacgg
     1441 tgagccaact caaggagacc tttggtaaga tgaagactct ctcttttgaa ctgctaccag
     1501 gtcaaagtca tctcgtctct cactatgacg gtctgtctga tatgaccatt gatagacaag
     1561 gaaacagcct caacattgaa tttgatagtt ctcgctacca gtcagctgac attatcaagc
     1621 aaaccctgtc tgattttgaa atccgcgatt tgaagatggt ggatacggat attgaggata
     1681 ttatccgtcg cttctaccga aaggagctct aggatgatca aattgtggag acgttataaa
     1741 ccctttatca atgcaggggt tcaggagttg attacttacc gagtcaactt tattctctat
     1801 cggattggcg atgtcatggg ggcttttgtg gccttttatc tctggaaggc tgtctttgat
     1861 tcttcgcaag agtctttgat tcagggcttc agtatggcgg atatcaccct ctacatcatc
     1921 atgagttttg tgaccaatct tctgactaga tccgattcgt cctttatgat tggggaggag
     1981 gtcaaggatg gctccattat catgcgtttg ttgcgaccag tgcattttgc ggcctcctat
     2041 cttttcaccg agcttggttc caagtggttg atttttatca gcgttggcct tccattttta
     2101 agtgtcattg tcttgatgaa aatcatatcg ggtcaaggta ttgtagaggt gctaggatta
     2161 actgtcattt atctttttag cttaacgctc gcctatctga ttaacttttt ctttaatatt
     2221 tgctttggat tttcagcctt tgtgtttaaa aatctttggg gttccaacct acttaagact
     2281 tccatagtgg cttttatgtc ggggagtttg attcccttgg cattctttcc aaaggttgtt
     2341 tcagatattc tctccttttt gcctttttca tccttgattt atactccagt tatgatcatt
     2401 gttggaaaat acgatgccag tcagattctt caggcactcc ttttgcagtt cttctggctc
     2461 ttagtgatgg tgggattgtc tcagttgatt tggaaacggg tccagtcctt tatcaccatt
     2521 caaggaggtt agtatgaaaa aatatcaacg aatgcatctg atttttatca gacaatacat
     2581 caaacaaatc atggaatata aggtggattt tgtggttggt gtcttgggag tctttctggc
     2641 tcaaggcttg aatctcttgt ttctcaatgt catctttcaa catattccat tcctagaagg
     2701 ctggaccttt caagagatag ctttcattta tggattttcc ttgattccca agggaatgga
     2761 ccatctcttt tttgacaatc tctgggcact agggcaacgc ctagtccgaa aaggggagtt
     2821 tgacaagtat ctgactcgtc ccatcaatcc tctctttcac atcctagttg aaacctttca
     2881 gattgatgcc ttgggtgaac tcttagtcgg tggtatttta ttgggaacaa cagtgaccag
     2941 cattgtttgg actcttccga aattcctgct tttcctagtt tgtattcctt ttgcgacctt
     3001 gatttatact tctcttaaaa tcgcaacagc cagtatcgcc ttttggacta agcagtcagg
     3061 cgccatgatt tacatcttct atatgttcaa tgactttgct aagtatccga tttctattta
     3121 caattctctt cttcgttggt tgattagctt tatcgtgcct ttcgccttta cagcctacta
     3181 tccagctagc tatttcttac aggaaaagga tgtgttcttt aacgtaggag gtttgatgtt
     3241 gatttctctg gttttctttg ttatttccct taaactttgg gataagggct tagattccta
     3301 cgaaagtgcg ggttcgtaaa agctaaagta agactaaaat caagaaagaa acttatgatg
     3361 tttgtaattg aagaagtcaa ggatgaaaat caaaaaaagg cagttgtagc tgaggttttg
     3421 aaggatttgc cagaatggtt tggaatccca gaaagcacac aagcctatat agaaggaacc
     3481 acgacactgc aagtttggac cgcctatcag gagagtgatt tgactagatt tgtaagctta
     3541 tcctattcga gtgaagattg tgcagagatt gattgtctcg gcgtaaaaaa gcttatcaag
     3601 gtagaaaaat tgggagccaa ttgcttgcta ctttagagag tgaagctcgt aaaaaagttg
     3661 gttatctgca ggtcaaaaca gtggcagaag gttctaataa agattatgat cgaacaaatg
     3721 acttttatcg aggtcttggc tttaaaaagt tagagatttt tcctcaacta tggaatccgc
     3781 aaaatccttg tcagattttg attaaaaagc ttgaataata ttacttgaca tctattctca
     3841 gagtgccata ctgtaagtgt aatcgccgat ttagctcagt tggtagagca aggcactcgt
     3901 aaagcctagg ttataggtag ataaacgact gaggagttga aaaaatagat aggtagaaga
     3961 taaccgttaa gccttattct tagcggttat ttatattgtt taatagcgct gatatttcat
     4021 caattatgcc tgttttcgtg tttctggtag ttgttcaagt ttattgctac tatttttgat
     4081 ggtatgaatg tgcttataat gtatcccggt taacgaaagt tttggactta tactctt
//
LOCUS       DAVBCW010000131         4119 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVBCW010000131 DAVBCW010000000
VERSION     DAVBCW010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.131"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            98..1357
                     /locus_tag="VQ514_001886"
     CDS             98..1357
                     /locus_tag="VQ514_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HET2500614.1"
                     /translation="MKVFQHVNIVTCDQDFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQIQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    1433..1568
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            1607..2107
                     /locus_tag="VQ514_001887"
     CDS             1607..2107
                     /locus_tag="VQ514_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HET2500615.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            2183..2551
                     /gene="rplL"
                     /locus_tag="VQ514_001888"
     CDS             2183..2551
                     /gene="rplL"
                     /locus_tag="VQ514_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HET2500616.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKTKLEEAGASVTLK"
     gene            2738..2908
                     /locus_tag="VQ514_001889"
     CDS             2738..2908
                     /locus_tag="VQ514_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500617.1"
                     /translation="MQKEGQSSRQFVKSHLMTIMGVDIVKATQLVDEMEQVGLIRFDE
                     FGNVGILVLEGQ"
     gene            2908..3156
                     /locus_tag="VQ514_001890"
                     /pseudo
     CDS             2908..3156
                     /locus_tag="VQ514_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            3170..3510
                     /locus_tag="VQ514_001891"
                     /pseudo
     CDS             3170..3510
                     /locus_tag="VQ514_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812122.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3503..3883
                     /locus_tag="VQ514_001892"
     CDS             3503..3883
                     /locus_tag="VQ514_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HET2500618.1"
                     /translation="MIDLYLSKNRQRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFVEIILKNPELTIRLPLAVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            3896..4039
                     /locus_tag="VQ514_001893"
     CDS             3896..4039
                     /locus_tag="VQ514_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500619.1"
                     /translation="MAERFWENLSIILAERNISWIELTRKMFAGEFHYPSELNRLYQK
                     IRH"
BASE COUNT         1324 a          722 c          884 g         1189 t
ORIGIN      
        1 agatagaact gacgaagtca gtaaccacac ctacggcaaa gtgaatctga agtggtttga
       61 agagagtaca acttgtcttt tagaaaagga gcctataatg aaagtctttc agcatgtaaa
      121 tatcgtgact tgtgatcaag atttccatgt ttatcttgat ggaatcttag cagtcaagga
      181 ttctcaaatc gtctatgtcg gtcaagataa gccagcgttt ttagagcaag ctgagcagat
      241 tatagactat cagggagctt ggattatgcc tggtttggtc aattgtcaca cccattctgc
      301 aatgacaggt ctgagaggga tccgagatga cagcaatctc catgaatggc tcaatgacta
      361 tatctggcca gcagaatctg agtttactcc cgacatgact accaatgcgg tcaaagaagc
      421 cctgacagag atgctccagt caggaacaac aacctttaac gatatgtata atcccaatgg
      481 tgtggatatc cagcaaattt atcaggtggt gaaaacttcc aagatgcgtt gttatttttc
      541 tccgactctc ttttcttcag agacagaaac aactgctgag actataagca gaactcgatc
      601 catcatagac gaaatcttaa aatataaaaa tccaaatttc aaggttatgg tagcacctca
      661 ttctccgtat agctgcagta gagacttgct ggaagcgagt ttggaaatgg caaaagagct
      721 aaatattccg ctccatgtcc atgtggcgga gaccaaggag gagtcaggaa ttatcctcaa
      781 acgatacggc aaacgccccc ttgcttttct tgaagaactg ggttatttag atcatccgtc
      841 cgtatttgct cacggggtcg aattaaacga gagagaaatt gaacgcttgg catcttctca
      901 agtggctatc gcccacaatc ctatcagtaa cctcaaactg gcatcaggaa ttgctccaat
      961 tatccagatc caaaaagcgg gagtagcagt cggaattgcg actgactcgg ttgcttccaa
     1021 taacaatcta gatatgtttg aggaaggaag gactgcagct cttcttcaga agatgaaaag
     1081 tggggatgcc agccagtttc caatcgaaac agctctcaag gtactgacaa tcgaaggggc
     1141 taaggtcctt ggaatggaaa atcagatagg aagtctggaa gtcggcaagc aagcagattt
     1201 tctggtcatt caaccacaag ggaaaattca tctccaacct caggaaaata tgctgtctca
     1261 cctggtttat gcagttaaat ctagtgatgt agatgatgtt tatatcgccg gagaacaggt
     1321 tgttaagcaa ggtcaagtcc tgacagtaga actttaaaag aaaaatcacg aaaaatttta
     1381 aaaaaagttt gcaaaaatct tgcattcttt ttttgactat gctatactta tatacggttt
     1441 aaaaaaactg cctaagacag taggggagct cgactcataa atatcctacc gaggacaaga
     1501 cgtatcatgt aaaaagaagc gtattgtact ttcgtgtcta ggtttgggcg cgtttttctt
     1561 tttgaaaaat tccccaagca aaataattac ggaggtgaac acactaatga gtgaagcaat
     1621 tattgctaaa aaagcggaac tagttgacgt agtagctgaa aaaatgaaag ctgctgcatc
     1681 tatcgtcgtt gtagacgctc gtggtttgac agttgagcaa gatacagttc ttcgtcgcga
     1741 gcttcgtgga agcgaagttg agtataaagt tattaaaaac tcaatcttgc gtcgtgcagc
     1801 tgaaaaagct ggtcttgaag atcttgcatc tgtatttgtt ggaccatctg cagtagcatt
     1861 ttctaatgaa gatgttatcg caccagcgaa aatcttgaac gacttttcta aaaacgctga
     1921 agcacttgaa attaaaggtg gtgcaatcga aggcgctgtc gcatctaaag aagagattct
     1981 tgcacttgca actcttccaa accgcgaagg acttctttct atgctccttt ctgtacttca
     2041 agcgccagtg cgcaacgttg ctcttgcagt caaagcggtt gcagaaagca aagaagacgc
     2101 ggcttaatct taagctacac agcgtagcct agctacgaaa aaaactatta taaaatttaa
     2161 aacttatttg gaggaaataa caatggcatt gaacattgaa aacattattg ctgaaattaa
     2221 agaagcttca atccttgaat tgaacgacct tgtaaaagct atcgaagaag aatttggtgt
     2281 aactgcagct gctcctgtag ctgttgctgc agctgatgca gctgatgctg gtgctgctaa
     2341 agattcattc gacgttgaat tgacatctgc aggcgacaaa aaagttggcg ttatcaaagt
     2401 tgtacgtgaa atcactggtc ttggtcttaa agaagctaaa gaacttgttg acggtgcacc
     2461 agcacttgtt aaagaaggcg ttgcaactgc agaagctgaa gaaatcaaaa ctaaattgga
     2521 agaagctgga gcttcagtta ctcttaaata atagagtatc gcaatttgat tgcggaatcc
     2581 ctatttacca gtctttgaga gcgataagcg attgatagaa actgataaat tgatttggtt
     2641 tcctgccaaa aattctttgg caggattttt gttttaataa actttcgggg gtagacacta
     2701 gattgtctac cccttttttt gtgtttcgga ggtaattatg caaaaggaag gacagtcatc
     2761 acgtcaattc gtgaagagtc atctcatgac tatcatggga gttgatattg taaaagcaac
     2821 tcagttagtt gatgaaatgg aacaagtagg tttaatccga tttgatgaat ttggaaatgt
     2881 tggaatttta gtcttggagg gacaataatg aagtgtatta ccgctaatca atatcagaca
     2941 tcagagcgtt attataagct ccccaaactt ttatttgaga gtgaacggta taagaatatg
     3001 aaactggagg ttaaggtggt ttactcagtt ttaaaagatc ggttggagtt gtctttgagc
     3061 aaaggttgga ttgatgagga tggggctatt tatttgattt attccaattc aaatttgatg
     3121 gcacttttag gctgttcaaa gtcaaaatta ctctccatgt gagtttgaag tgacattttt
     3181 agatgattac cataaaaaac ataactaccc actattttac gaatcctatc ttcaaaacgt
     3241 tatggaattc cttgaaagtc aagacataaa gaatggggtt gatgcctttg tagatgatca
     3301 tcaaaatctc gtttttgttt tatatggaca aggctatcga gccgagggaa aagagggaat
     3361 acttacaacc caagtaactg taaaagctta tgatgaagac aagaaaccga ttaacttcgc
     3421 aaatttatta gattccttaa tttattagat tccttaatcg tgtcagaata tcaaatggaa
     3481 ccgaatcttt gggaggtctc ccatgattga tctctatcta agtaaaaata gacaaagaaa
     3541 tcaacttctt ttagatttct tccaaaacta tggcatagag gtatcttgtc attcagtttc
     3601 tgaaatgaca aaggacaaat taattgagat gatgagctat tcttcagatt gttttgagtt
     3661 tttatctccc aatttattac gatttaagaa ccgtgacaat ctaagattaa cggatttcgt
     3721 tgagataatt ttaaaaaatc ctgaactaac catcagactt cctcttgcgg tttcaaataa
     3781 gcgagtttat ccaagtctga atttggaaga ggctagagct ctattgccaa gagatacgaa
     3841 acaattgatt tacatggcgc aaacacatta tttatctaac taaaggagaa gcaatatggc
     3901 tgaacgattt tgggagaact tgtccattat tttggctgaa agaaatatca gctggattga
     3961 gttaaccaga aaaatgtttg cgggagagtt tcactatcca agtgaattga atcgtttgta
     4021 tcaaaagatt cgccactaga agatggaaca atgaatgcct caaagtccat gggtagaaag
     4081 gattgtgcag gtattagatt tagattatga agatttatt
//
LOCUS       DAVBCW010000132         4092 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVBCW010000132 DAVBCW010000000
VERSION     DAVBCW010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.132"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            509..1162
                     /gene="rr06"
                     /locus_tag="VQ514_001894"
     CDS             509..1162
                     /gene="rr06"
                     /locus_tag="VQ514_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HET2500620.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDIWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            1159..2499
                     /gene="hk06"
                     /locus_tag="VQ514_001895"
     CDS             1159..2499
                     /gene="hk06"
                     /locus_tag="VQ514_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HET2500621.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILFLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGEVGKQINGMYEHLLKV
                     IHELESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIA
                     KSINKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIEN
                     QLTDATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQE
                     LEHLFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            2582..3109
                     /locus_tag="VQ514_001896"
     CDS             2582..3109
                     /locus_tag="VQ514_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HET2500622.1"
                     /translation="MVLAIILVTFFIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAMVHKTIFDGMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYVTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            3188..3991
                     /locus_tag="VQ514_001897"
                     /pseudo
     CDS             3188..3991
                     /locus_tag="VQ514_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RICH domain-containing protein"
BASE COUNT         1429 a          597 c          842 g         1224 t
ORIGIN      
        1 tttgaagaga ttttcgaaga gtatgaaact aaaacctata gcttctaaac gatccgtggt
       61 tttcaccatt caacacaaaa ttcatatgtt tattaccctc cgtcgtattt gtcttagagc
      121 gtgtgtagta gaaaaagagc agtcttatct gaaattttta ttctttcaaa agagacctgt
      181 ttcttttttg catgtcaaat ccgttctagc tggtatttga aaaatcaaac taatattcaa
      241 tgaaaatcaa agaacaaact aggcagctag ccacaggttg ctcaaaacac tgttttgagg
      301 ttgtagatag agctgacgta gtttgaagag attttcgaag agtataagct gcaagatgaa
      361 tgattttctt gtattgacgt tgttgttgac aaaaagtagc ggataaatga aatccattcc
      421 attatcatag atgataggct ggtaggaaat tttcaaatag catacaggaa atagatgtat
      481 ggagttctgg tagtagaaag ggagagagat gaacatttta gttgcagatg acgaggaaat
      541 gattagagaa ggaattgcag catttctgac agaagagggt tatcatgtca ttatggctaa
      601 ggatggacaa gaggtcttgg aaaaatttca agatctccct atccatctca tggtattgga
      661 tttaatgatg cctaggaaga gtggttttga agtgttaaaa gaaatcaatc aaaagcacga
      721 tattcctgtc atcgtcttga gtgctctggg agatgaaact actcagtcac aggtatttga
      781 tctctatgct gatgatcatg tgacaaaacc tttttctttg gtactgcttg tcaagcgtat
      841 taaggcgctt atcagacgtt actacgtcat agaggatatt tggcgatatc aggatgtaac
      901 agtggatttt acctcttaca aagcacatta taaaaatgaa gaaattgatc tcaaaccaaa
      961 ggaattactg gtactaaagt gtttgattca gcataaaaat caagttttaa gtagagagca
     1021 gatattggaa gaaatttcaa aagatgtagc tgatttacct tgtgataggg tcgttgatgt
     1081 ctatattcgt actcttcgca aaaaattagc tttagattgt atcgtgactg tgaaaaatgt
     1141 tgggtataag attagcttat gataaaaaat cctaaattat taaccaagtc ttttttaaga
     1201 agttttgcaa ttctaggtgg tgttggtcta gtcattcata tagctattta tttgaccttt
     1261 cctttttatt atattcaact ggagggggaa aagtttaatg agagcgcaag agtgtttacg
     1321 gagtatttaa agactaagac atctgatgaa attccaagct tactccagtc ttattcaaag
     1381 tccttgacca tatctgctca ccttaaaaga gatattgtag ataagcgact ccctcttgtg
     1441 catgacttgg atattaaaga tggaaagcta tcaaattata tcgtgatgtt agatatgtct
     1501 gttagtacag cagatggtaa acaggtaaca gtgcaatttg ttcacggggt ggatgtctac
     1561 aaagaagcaa agaatatttt gtttttgtat cttccatata catttttggt tacaattgct
     1621 ttttcctttg ttttttctta tttttatact aaacgcttgc tcaatcctct tttttacatt
     1681 tcagaagtga ctagtaaaat gcaagatttg gatgacaata ttcgttttga tgaaagtagg
     1741 aaagatgaag ttggtgaagt tggagaagtt ggaaaacaga ttaatggtat gtatgagcac
     1801 ttgttgaagg ttattcatga gttggaaagt cgtaatgagc aaattgtaaa attgcaaaat
     1861 caaaaggttt cctttgtccg tggagcatca catgagttga aaaccccttt agccagtctt
     1921 agaattatcc tagagaatat gcagcataat attggagatt acaaagatca tccaaaatat
     1981 attgcaaaga gtataaataa gattgaccag atgagccact tattggaaga agtactggag
     2041 tcttctaaat tccaagagtg gacagagtgt cgtgagacct tgactgttaa gccagtttta
     2101 gtagatattt tatcacgtta tcaagaatta gctcattcaa taggtgttac aattgaaaat
     2161 caattgacag atgctaccag ggtcgtcatg agtcttaggg cattggataa ggttttgaca
     2221 aacctgatta gtaatgcaat taaatattca gataaaaatg ggcgcgtaat catatccgag
     2281 caagatggct atctctctat caaaaataca tgtgcgcctc taagtgacca agaactagaa
     2341 catttatttg atatattcta tcattctcaa atcgtgacag ataaggatga aagttccggt
     2401 ttgggtcttt acattgtgaa taatatttta gaaagctatc aaatggacta tagttttctc
     2461 ccttatgaac acggtatgga atttaagatt agcttgtaga cagattagtt ttttattaaa
     2521 gttcatatag ggttaacata agtgtgttat tctttgtgta gataaaagaa aggatactaa
     2581 tatggtatta gcgattattt tagtaacatt ctttattcga ttgatttttt taaagcgttc
     2641 gatagagaat gagaaacgaa tccttagcaa tggcgggaaa gaatttggag ttgagaatac
     2701 aaaacgatta actatggctc atattgtttt ttatctctct tgcttggttg aggcaatggt
     2761 gcacaagaca atttttgatg gcatgggcat ggttggttta gtcttgctta ttttttctat
     2821 gctgatgtta atgttggtga ttcacttgtt gggagatatt tggacagtga agcttatgct
     2881 tgtcaataat cacaaatatg tagatcatat cttgtttagg acagtaaaac atcctaatta
     2941 ctttttaaat attcttcctg agttgattgg cttgaccttg ttgagtcatg cttatgtgac
     3001 ttttgtttta gtttttccag tttatgcagt tattttgtat cgacgaatag ctgaagagga
     3061 aaagctatta catgaagtta taatcccaaa tggaagcata aagagataaa tacaaaattc
     3121 gatttatata cagttcatat tgaagtgata tagtaaggtt aaagaaaaaa tatagaagga
     3181 aataaacatg tttgcatcaa aaagcgaaag aaaagtacat tattcaattc gtaaatttag
     3241 tattggagta gctagtgtag tagttgctag cttgttctta ggaggagtag ttcatgcaga
     3301 aggggttaga agtggggata cccccaaggt tacatctagt ggggatgaag tcgagtcgca
     3361 tctaaaaaaa atattgagtg agatccaaac acaattagat agaaaacaac atgccaaaac
     3421 tgtagcgcta attaacaagt tgcaagacat taagagaacg tattcgtata aattaaatgt
     3481 tttagaagag aagccgaaag ctgagttgac gtcaaaaaca aaagaagagt taaccgcagc
     3541 ttttgagcaa tttaaaaaag atacattacc aacagaacca ggaaaaaagg tagcagaagc
     3601 tgagaagaag gttgcagaag ctgagaaaaa agccgaggat caaaaagaag aagatcgtcg
     3661 taactaccca accaatactt acaaaacgct tgaacttgaa attgctgagt tcgatgtgaa
     3721 agttaaagaa gcggagcttg aactattaaa agagaaagct aagaaacctt taaacgaggg
     3781 cacaattaag caagcaaaag agaaagttga gagtaaaaaa gctgaggcta caaggttaga
     3841 aaacatcaag acagatcgta aaaaagcaga agaagaagct aaacgaaaag cagcagaaga
     3901 agataaagtt aaagaaaaac cagctgaaca accacaacca gcgccggctc ctcaaccaga
     3961 aaaaccagct ccaaaaccag aaaaaccagc tccagctcca aaaccagaga atccagctcc
     4021 aaaaccagaa aaaccagctc cagctccaaa accagagaat ccagctgaac aaccaaaagc
     4081 agaaaaacca gc
//
LOCUS       DAVBCW010000133         3964 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVBCW010000133 DAVBCW010000000
VERSION     DAVBCW010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.133"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(<1..370)
                     /locus_tag="VQ514_001898"
     CDS             complement(<1..370)
                     /locus_tag="VQ514_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2500623.1"
                     /translation="MLLVLVSLVHSFGSVSIIAHTIHQKFNLKVPNYRQEEDWARMGL
                     PITRKEISNWHIKASQYYLESLYKLLREKLLEQPLLHADETSYRVLESDSHLTYYWTF
                     LSGKAENQAITLYHHDQRRSG"
     gene            complement(446..1297)
                     /gene="rfbD"
                     /locus_tag="VQ514_001899"
                     /pseudo
     CDS             complement(446..1297)
                     /gene="rfbD"
                     /locus_tag="VQ514_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647909.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
     gene            complement(1363..2412)
                     /gene="rfbB"
                     /locus_tag="VQ514_001900"
     CDS             complement(1363..2412)
                     /gene="rfbB"
                     /locus_tag="VQ514_001900"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HET2500624.1"
                     /translation="MTEYKNIIVTGGAGFIGSNFVHYVYENFPDVHVTVLDKLTYAGN
                     RANIEEILGNRVELVVGDIADAELVDKLVAQADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGIYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILTKGQIGETYLIGADGEKNNKEVLELILKEMGQATD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKATIKWYTDNQEWWKAEKE
                     AVEANYAKTQEIITV"
     gene            complement(2422..3018)
                     /locus_tag="VQ514_001901"
     CDS             complement(2422..3018)
                     /locus_tag="VQ514_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HET2500625.1"
                     /translation="MTDNFFGKTLAARKVEAIPGMLEFDIPVHGDNRGWFKENFQKEK
                     VLPLGFPESFFAEGKLQNNVSFSRKNVLRGLHAEPWDKYISVADGGKVLGSWVDLREG
                     ETFGNTYQTVIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYA
                     DPSLGIEWENLEEADVSEADKHHPLLKDVKPLKKEDLE"
     gene            complement(3019..3876)
                     /gene="rfbA"
                     /locus_tag="VQ514_001902"
     CDS             complement(3019..3876)
                     /gene="rfbA"
                     /locus_tag="VQ514_001902"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HET2500626.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIRDILIISTPQDLHRFQELLQDGSEFGIKLSYAEQPSTDGLAIGEEFISDDSVALIL
                     GDNIYHGPGLSTMLQKAAKKEKGATVFGYQVKDPERFGVVEFDTDMNAISIEEKPEYP
                     RSNYAVTGLYFYDNDVVEIAKSIKPSPRGELEITDVNKAYLDRGDLSVELMGRGFAWL
                     NTGTHESLLEASQYIETVQRMQNVQVANLEEIAYRMGYISREDVLALAQPLKKNEYGQ
                     YLLRLIGEA"
BASE COUNT         1107 a          917 c          719 g         1221 t
ORIGIN      
        1 aaccactccg acgctgatca tggtggtaca gcgtgattgc ttgattctca gctttcccag
       61 acaaaaaagt ccaatagtag gtcagatggc tatcactctc tagaacccga taagaggttt
      121 catccgcatg aagaagaggt tgttctaaca acttttctcg taaaagctta taaagggact
      181 ctaaatagta ttgacttgcc ttgatatgcc aattagagat ttccttacgt gtgattggta
      241 aacccatcct agcccaatct tcttcttggc gataattggg taccttcaga ttaaacttct
      301 gatggatggt gtgagcgata atagaaactg agccaaagct atgcaccaaa gagaccagca
      361 ccaacaacaa gataattgta catcttaagc tcctttggag ttcattatct gcctctttta
      421 ttagactaga aaatcattct actacttatc tcacttcttg tttgtaaaat tcttgcaatg
      481 catcttgcca agttggaata acaaatccag tagctttggc tttggccagg ctcatcgttg
      541 agtttagcgg acgttgagct ttggctggaa attgactgga atctactggc ttgacttcga
      601 catctgtatc tttcaaaatt tcaactgcaa aatcatacca tgttgtgtct tctgtcgcat
      661 catttgacaa atgataataa ccaaattcct tacgattttc agctaggtag atcatgaatt
      721 cagccaaggt acgagtccaa gtcggacgac cgtactggtc atttacaact gttaaagtct
      781 tatgagtttt cgcaagattt tgcatggtaa aaacgaagtt tttgccataa tttccaaata
      841 cccaggcagt acggataata tagaaattag acacatgctt ctcaactaac tcttccccca
      901 tacgcttagt gcgtccatat tctgtctgtg gatctggtcg gtcatcaact tcccactctt
      961 gtccaactgg tttcttaccg tcaaagacct agtccgtaga aatataaact agagttgcac
     1021 catgcttttc agatgctttt gcgacatttt ttgtccccat cacattgatg gcgaagtcca
     1081 actcttttcc ttcatcctct gctgcatcaa cagcggtgta ggctgcacaa tggtagacta
     1141 aagtcggttt cacctcttca aaaactttct caaccatttc ttcattggta atgtccatct
     1201 cagccacatc tactgccacg tattcttcat tacgttcatc caataaatag cgaagttccg
     1261 ttcctaattg gccatttgcc cctgtaatta aaatcatctc tagacctttc taatatgtaa
     1321 ctcttcccaa tatagcaata aaaagcagcg atttcctgct ttttatactg taataatctc
     1381 ctgagtctta gcataattgg cttcaacagc ttctttttct gctttccacc attcttggtt
     1441 atctgtatac cacttgattg ttgccttgag cccagcttca aagttggtaa attcaggttt
     1501 ccaccccaac tcatcacgga gcttgctggc atcaatcgca tagcgaaggt catgtcctgc
     1561 acggtcagtc acatgatcat aggcatccgt agcttgtccc atttccttaa ggataagttc
     1621 caaaacttcc ttattgttct tctcaccatc agccccaatc aagtaggttt caccgatttg
     1681 cccttttgtc aagattgtcc aaactcctga agaatggtca ttggtatgaa tccagtcacg
     1741 aacgttctta ccttcaccgt aaagttttgg cttaattcca cttaggatgt tagtaatctg
     1801 acgtgggatg aatttttcga tatgttgata aggaccgtag ttatttgaac agttggaaat
     1861 cgttgccttg actccaaaag aacgcaccca ggctttgaca atcaagtctg atgcggcctt
     1921 ggttgatgag tacggagagc ttgggttgta ttttgtttca gcagtaaatt tctcacctgg
     1981 tccttcacca tgacctggca aatcttctcg taaagggaga tccccataaa cttcatctgt
     2041 cgatacatgg tggaagcgaa tatcatactt acgagcagct tctaaaagag tatagattcc
     2101 aatgaagtta gtatgaataa atggcgatgg atcattgagc gaattatcat tgtggctttc
     2161 cgctgcataa tgaacgatag catctgcttg agcaaccaac ttgtctacca actccgcatc
     2221 agcaatgtca ccaacaacta actcaacacg attgcctaaa atttcctcaa tattcgcgcg
     2281 gtttccagca taagtcaact tatctaggac tgtcacgtga acatctggaa agttctcgta
     2341 aacataatgg acaaagttag aaccgataaa gccagctcca cctgtcacga taatattttt
     2401 gtattcagtc atattctttc cttattccaa atcttctttt ttcaaaggct tcacgtcctt
     2461 aagtagggga tgatgtttgt ctgcttcaga tacgtctgct tcttccagat tttcccattc
     2521 aataccaagg cttggatcag cgtagttcac aaaggcatac ttgggtttga gttcaagagc
     2581 ccagtaatca ttgaccagat agctatatga cactgtatct gataaaactt ggaagccatt
     2641 agctacgcct cgaggaacaa agattccctt gcttgcatca attactgtct gataggtatt
     2701 cccaaaggtt tcaccctcgc gtagatcaac ccaagaaccc agaactttcc ctccatctgc
     2761 tacagagatg tacttatccc aaggctctgc gtggaggcct cgaaggacat ttttacgtga
     2821 gaaggataca ttgttttgca attttccttc tgcaaagaaa gactctggaa atccaagtgg
     2881 aagcactttt tccttttgga aattttcttt aaaccagcca cgattatctc catgaacggg
     2941 gatatcaaac tccaacatgc ctggaatagc ttcaaccttg cgtgccgcaa gcgtcttacc
     3001 gaaaaaatta tctgtcatct atgcttctcc aatcaaacgg agcagatact gtccgtattc
     3061 atttttctta agtggttggg ctaaggccaa tacatcttct cgactgatat agcccatacg
     3121 gtaagcaatt tcttctaagt ttgctacctg aacattttgc atccgttgca ctgtttcgat
     3181 gtactgtgaa gcctctagta aactttcatg agttccagta ttcagccaag caaagccacg
     3241 tcccataagc tcaacagaca aatcaccacg atctaggtaa gccttgttta catctgtaat
     3301 ttccagttca ccacgagggc ttggtttaat actcttggca atttcgacaa catcgttatc
     3361 atagaaatac agtcctgtca ctgcatagtt ggagcgagga tactccggtt tttcttctat
     3421 ggaaatagca ttcatgtctg tatcaaactc aaccacacca aaacgctctg gatccttcac
     3481 ttggtagcca aaaacagtcg cacctttctc tttcttggct gctttttgaa gcattgtgct
     3541 cagaccagga ccatggtaga tattgtcgcc taaaatcaag gcaacgctat cgtcagaaat
     3601 aaactcttcc ccaattgcca aaccatctgt acttggttgc tctgcataag aaagtttgat
     3661 cccaaactca gatccgtctt gaagaagctc ttgaaatcga tgtaaatcct gtggagtgga
     3721 aataatcaaa atatccctaa tcccagccaa catcaatgtt gaaagtgggt agtaaatcat
     3781 cggtttatca taaaccggca taagttgttt tgacgcagcg cgagtcaaag gatataaacg
     3841 tgtccccgaa ccacctgcta gaataatacc tttcatagta gggtaccttt ctttgttatt
     3901 ttagagtcaa ttactattaa ttgtgtactg ctctaaaaag aaaacaatca tcccatgttt
     3961 tata
//
LOCUS       DAVBCW010000134         3747 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVBCW010000134 DAVBCW010000000
VERSION     DAVBCW010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.134"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            443..1804
                     /gene="trpE"
                     /locus_tag="VQ514_001903"
     CDS             443..1804
                     /gene="trpE"
                     /locus_tag="VQ514_001903"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HET2500627.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQAIDRDPLDFLYEVTHESQHHSDLPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEGMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKALATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMEVELFRY
                     VMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRSVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            1801..2367
                     /locus_tag="VQ514_001904"
     CDS             1801..2367
                     /locus_tag="VQ514_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HET2500628.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMICDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            2378..3382
                     /gene="trpD"
                     /locus_tag="VQ514_001905"
     CDS             2378..3382
                     /gene="trpD"
                     /locus_tag="VQ514_001905"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HET2500629.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEDYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            3379..>3747
                     /locus_tag="VQ514_001906"
     CDS             3379..>3747
                     /locus_tag="VQ514_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase TrpC"
                     /protein_id="HET2500630.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDC
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIR"
BASE COUNT         1107 a          718 c          921 g         1001 t
ORIGIN      
        1 atacctacgg caaggcgacg ttgacgcggt ttgaagagat tttcgaagag tataaaggtg
       61 taccgagatg aacatctctt catcaatgtc tctggtcgtg gaggcaagga cctagctgcg
      121 attgcagatt actagaagct aaaaaataat agatggaaaa attacagtct tttctctcac
      181 agagagcttg tggttgctga aaacaagcag agaaagctgt aaagttgggt tcatctgaaa
      241 cgagagaaga aaacatcatt ctcaagggag tgccctttat cgcacagcct gttacaggag
      301 atatctgaga caggaatgag agataggaga aatcctataa ttgaggtggc accgcgaatt
      361 tcgtcctcac gcaagttatt ttgcgtgggg atttttcata cagtcgccac gaactagaat
      421 tagaactgaa agggaaatga caatggaacg aatcattcat ggagatgtct tatcaccaat
      481 cttggcttat atgcgcttaa aggggcaaca taaggttatt ttagagagta ttccgagaga
      541 caaggaaaca gctcgttttt ctatcctagc ctataatcca gtttttgaga ttaagtttga
      601 aaatggagtt ctctatcaaa atggtcaagc gattgatcgg gatcccttgg atttccttta
      661 tgaagtgact catgagagcc agcaccattc agacctccct tttggtgggg gagccattgg
      721 ttttgttggt tacgatatga tttcgcttta tgaagaaatt ggtcaaatcc ctgaggatac
      781 aattgggacg ccagacatgc atttctttgt ctatgagagc tatatggtct ttgaccacaa
      841 gaaggagaag atacatgtca tagaggatgc actttatagt gagcgcagtc aagaggcctt
      901 ggaaaaatcc ttgaaccaag tgcttgagga attacgcatt cctgctccaa atgaatttga
      961 agacttggat ttatcaccac ttgacttcaa accgcatatc gctccacata agtttgaggg
     1021 aatggtagaa acagctcgtg acttgattcg taacggagat atgttccaat gtgtactcag
     1081 ccagcgtttc tcagcagaag ttactggaaa tccatttgac ttctacagaa atctccgcgt
     1141 gactaatcca tctaattacc tttatttcta tgattttggg gattatcaaa tcatcggagc
     1201 tagtccagaa agtttggttt ctgtcaaaaa tggcatcgtg acaaccaatc cgattgcagg
     1261 gacgcgacca agaggggcta cggatgaaga agacaaggcc ttggcgactg acctcctctc
     1321 tgatgagaag gaaacagcag aacatcgaat gttggtagac ttggggcgta acgatattgg
     1381 ccgcatctct gaaacggcca gtgtccaagt tactaagtat atggaggtgg agctcttccg
     1441 ctacgtcatg catttgacca gtgtggtcaa gggacgtttg cttgcagaac tcactgccat
     1501 ggatgccttg aaagcaacgc ttcctgctgg aaccgtttct ggagcaccaa aaattcgagc
     1561 tatgagacgc atctatgaac tggaaacgga aaaacggagc gtatatgcag gagcaatcgg
     1621 ctacttgtct gcgacgggtg atatggattt ggccattgcc atccgaacca tgattctcaa
     1681 aaatcaaaga gcctatgtgc aggctggggc agggattgtc tacgactcta tcgcccaaaa
     1741 cgaataccaa gaaaccatta acaaagcaaa atctatgact agaattggag aactaagacc
     1801 atgattttat tgattgacaa ctatgattct tttacctata acttggccca atacattggg
     1861 aattttgcag aagttcaggt tttgagaaat gatgattcca agctgtatga agaagctgaa
     1921 aaagcagatg gtctggtctt ttctcctggt cctggttggc cagttgatgc tggaaagatg
     1981 gaagacatga tttgtgattt tgcaggcaag aagccgattc tagggatttg tttgggtcac
     2041 caagccattg cagaagtctt tggtggtaag ctaggtttgg ctccaaaagt catgcatggg
     2101 aaacagagca atatcaactt tgaagcgcca tctgttttgt atcaaggtat tgaggatgga
     2161 cgtgcggtca tgcgttatca cagtattttg attgaggaaa tgccagaaga ctttgaagtg
     2221 acagctcgtt cgactgatga ccaagccatc atggggattc aacataaaaa cctaccgatt
     2281 tatggcttcc agtaccatcc agagagcatt ggaacgccag acggcttgtc ttctattcgg
     2341 aattttatcg agaaggttgt aaagtgagga aactaggatg aaagagatta ttgaaaaact
     2401 agcaaaattt gaaaatttat caggtgtgga aatgacggat gtcattgagc gtatcgtaac
     2461 tgggcgtgta actgaagcgc agattgcttc tctcctctta gctcttaaga tgaaggggga
     2521 aacacctgaa gaacgtacag ccattgccca agtcatgaga ggacatgccc agcatattcc
     2581 aactgagatt catgatgcta tggacaactg tggtacaggt ggggacaagt ctttcagttt
     2641 taatatttcc acaactgcag cctttgtctt ggctggtggc ggtattcaca tggccaagca
     2701 tggtaaccgc tcgatttctt ctaaatctgg ttccgcagat gtcctcgaag ccttgggaat
     2761 caatcttgac ctcaaaccag ctgaactagg taaggtcttt gataaaactg gaatcgtctt
     2821 tctcttcgct aaaaatatgc acccagctat gaaatacatc atgccagctc gtttggaatt
     2881 gggaattcca acgattatga acttgactgg tccactgatt catccaatgg ctttagaaac
     2941 acagcttctt ggaattagtc gtccagaact cctagaaagt acagctcagg ttttgaaaaa
     3001 tatgggtcgc aaacgtgcca tcgtggttgc tggaccagaa gggttggatg aagctggctt
     3061 gaacggaaca accaagattg cacttcttga aaatggcgaa atcagcttgt caagctttac
     3121 tccagaggat ttgggaatgg aagactatgc tatggaagat attcgtggtg ggaatgctca
     3181 ggaaaatgca gaaattttgc ttagcgttct gaaaaacgaa gcaagtccat tcttggaaac
     3241 gacagtcttg aatgctggtc ttggtttcta tgctaatggt aagattgata gcatcaagga
     3301 aggagttgcc ttggcccgtc aagtgattgc tagaggcaag gcccttgaaa aactcagact
     3361 gttacaggag taccaaaaat gagtcaggaa tttttagcac gaatcttaga gcagaaggcg
     3421 cgtgaggtgg agcagatgaa gctggagcaa atccagcctc tgcgccagac ctatcgcttg
     3481 gcagaatttt tgaagaatca tcaggactgc ttgcaggtaa tcgctgaggt caagaaagct
     3541 agccctagtt tgggagatat caatctcgat gtggatattg tgcaacaggc ccagacttat
     3601 gaagaaaacg gagcagtgat gatttcggtg ttgacagatg aggttttctt taaagggcat
     3661 ttggattatc tacgggaaat ttccagtcag gtagagattc cgacgctcaa caaagacttt
     3721 atcatagatg aaaagcaaat catccgc
//
LOCUS       DAVBCW010000135         3727 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVBCW010000135 DAVBCW010000000
VERSION     DAVBCW010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.135"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(<1..1415)
                     /locus_tag="VQ514_001907"
     CDS             complement(<1..1415)
                     /locus_tag="VQ514_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec-dependent serine-rich glycoprotein
                     adhesin"
                     /protein_id="HET2500631.1"
                     /translation="MFKVMRGGVDTTQVMTETVEDKVSHSITGLDILKGIVAAGTVIS
                     GTVATQTKVFTNESAVLEKTVEKTDALATNDTVVLGTISTSNSASSTSLSASESASTS
                     ASESASTSASTSASTSASESASTSASTSISASSTVVGSQTAAATEATAKKVEEDRKKP
                     ASDYAASVTNVNLQSYAKRRKRSVDSIEQLLASIKAAVFSGNTIVNGAPAINASLNIA
                     KSETKVYTGTGLDSVYHVPIYYKLTVTNDGSKLTFTYTVTYVDPTTRALGNLSKMGQK
                     MGHGYSIYNTGTSIQTMLTLGSDLGTPSDVKNYITDKDGVLVRSYNTSTMTTWGSGYT
                     WGNGAQMNGFFAKQGYGLTSSWTVPITGTDTSFTFTPYAAKTDNIRTNYFKGRGKVVE
                     SSTTSQSLSQSKSLSVSASQSASASASTSASESASTSASASASTSASASASTSASASA
                     STSASASASTSASESASTSASA"
     gene            complement(1413..1526)
                     /locus_tag="VQ514_001908"
                     /pseudo
     CDS             complement(1413..1526)
                     /locus_tag="VQ514_001908"
                     /inference="COORDINATES: protein motif:HMM:TIGR03715.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="KxYKxGKxW signal peptide domain-containing
                     protein"
     gene            complement(1767..2225)
                     /locus_tag="VQ514_001909"
     CDS             complement(1767..2225)
                     /locus_tag="VQ514_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2500632.1"
                     /translation="MGKERRKQGNLERKKRVVKKRKIPLEELKAFVEAHPDAFLREIA
                     ARFDCALPSVWAVLKQIKVILKKTTSFREQKPEKVSEFLDILDNLKDLPVLYIDETGI
                     NRYLYRPYAGAPRGEKVYDKISGRRFERTNEVEQKLNGSFLIRYIDSQIR"
     gene            complement(2192..2335)
                     /locus_tag="VQ514_001910"
     CDS             complement(2192..2335)
                     /locus_tag="VQ514_001910"
                     /inference="COORDINATES: protein motif:HMM:NF013842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2500633.1"
                     /translation="MAYSTDFKQGALDSIKEGHSHVEAAKFFGVGVRTLFTWEKKDVN
                     KGT"
     gene            2604..2984
                     /locus_tag="VQ514_001911"
     CDS             2604..2984
                     /locus_tag="VQ514_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET2500634.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            3083..3304
                     /locus_tag="VQ514_001912"
     CDS             3083..3304
                     /locus_tag="VQ514_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HET2500635.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            3321..3635
                     /gene="trxA"
                     /locus_tag="VQ514_001913"
     CDS             3321..3635
                     /gene="trxA"
                     /locus_tag="VQ514_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET2500636.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
BASE COUNT         1121 a          755 c          719 g         1132 t
ORIGIN      
        1 gctgacgcac tcgttgatgc agattcagac gctgaggtac ttgctgaagc tgaggcactc
       61 gttgatgcgg aagctgacgc actcgttgat gctgaggctg aggcacttgt acttgctgag
      121 gctgatgcgc tggttgaggc cgattcagaa gctgaggtgc ttgctgaggc tgaggcgctt
      181 tgactagcac ttactgagag tgacttagac tgtgaaagtg actgactggt cgtgctagat
      241 tcaactacct ttcctctacc cttgaagtag ttagttctaa tgttatctgt tttagcagcg
      301 taaggggtaa atgtaaagga tgtatccgtt ccagtaattg gtacagtcca agatgatgtt
      361 aatccatatc cttgcttagc aaagaaacca ttcatttggg caccatttcc ccaagtatac
      421 ccactacccc acgtcgtcat tgtagatgta ttataggatc gaaccagtac accatctttg
      481 tcagtaatgt agttctttac atctgaaggg gtaccaagat cactgccaag ggttaacatt
      541 gtttgtattg aagtacctgt attatagata gaatacccat gacccatctt ttgacccatc
      601 tttgaaagat taccaagagc ccttgtggta ggatctacat acgtaaccgt ataggtaaag
      661 gtcagttttg aaccatcatt tgtcactgtc aatttatagt aaattggaac atgatatacc
      721 gaatctaaac ctgtacctgt ataaactttt gtctcacttt tagcaatgtt tagacttgca
      781 ttaattgcag gggcgccatt tacaatcgta ttgccagaaa aaacagcagc ttttatagaa
      841 gccagcaatt gctcgatgga atccactgaa cgcttgcgtc gcttagcata agattggaga
      901 ttgacatttg taactgatgc tgcataatca ctagctggtt tcttacgatc ttcttcgacc
      961 ttcttagcag ttgcttctgt agcggcagct gtttgtgatc ctaccacagt agatgatgca
     1021 gagatacttg tcgaagccga tgtacttgct gattctgatg cacttgtact cgctgaggtc
     1081 gaagcgctgg ttgaggctga ctcagatgca gatgtacttg ccgactctga agctgacaaa
     1141 ctagttgaac tcgctgaatt acttgtagat atcgtaccta gaactactgt atcatttgtt
     1201 gccaaagcat ccgttttctc tacagttttt tcaagtactg ccgactcatt tgtaaatacc
     1261 ttcgtttgag ttgcaacggt tccacttatg acagttcccg cagcaactat tcccttgagg
     1321 atatcaagcc cagtaattga atgacttact ttatcttcta ccgtttcggt catgacctga
     1381 gtagtatcaa caccaccacg catgacctta aacaagccaa aaagagaagt cgaggcccgt
     1441 agccaatgct tccctgattt gattaatttg aaccgagtta ctcggtctgt ttctctatat
     1501 tcacccttct gacgtctaaa aaatattttg aaatttcctt tcgagtatta ttttcatgta
     1561 tggatataaa taaattacca tactataatt atagaatagg gaacccaaaa tagcaagaaa
     1621 cttgaggtat ttttgctaaa aaaaaaaagc attttactat tttatatata tattataaga
     1681 taagtttttt ataaagacac gcaaatgagt ataaatcagc taatttttgg tttatattcc
     1741 tatttttatg cataattatt ctttcattat ctaatttgtg aatcaatata tctgattaga
     1801 aaactaccat tgagtttttg ctccacctca ttagtccgct caaaacgccg tccgctaatc
     1861 ttgtcataga ctttctcccc tctaggagcc cctgcataag gacgatagag gtagcggttg
     1921 attcccgttt cgtcaatata taggactggt aaatctttta ggttatccaa aatatcaaga
     1981 aactcagaaa ctttctcagg cttttgttcc ctaaaactgg tcgtcttttt taaaatgacc
     2041 ttaatctgct ttaaaactgc ccatacggag ggcaaagcac aatcaaaacg ggccgcaatt
     2101 tcccgtaaaa aagcgtctgg atgagcctct acaaaggctt tcaattcttc taaagggatc
     2161 ttacgctttt tgacgactcg ctttttccgc tctaagttcc cttgtttacg tctttctttt
     2221 cccacgtgaa gagagttctg acgccaacac caaaaaactt ggctgcctcg acatgactgt
     2281 gcccctcttt gatggaatct aatgctcctt gtttaaaatc agtactatat gccatagctt
     2341 tattagacca tgcttatttt aaactaagca actatagata gagaaatgaa ataaaatcta
     2401 aattcgaatg tgataaggag ttagaaattg aaacaatagg attcccccat tattcacaca
     2461 aaaataaaac cactaaaaaa cagagcttgc cagcattctt gaaaagtagt ataattattg
     2521 catgcgcaat catcttgtga cgcagagact gcccgttaat ggactttctt ctatattatg
     2581 actctaaaaa gaaaggagac actatgacct atttggaaaa atggtttgac ttcaatcggc
     2641 gtcagaaaga aattgaaagt ctcttggaag agaccattgc ccaacagagc gagcaaagtc
     2701 tgaccttgaa agagttctac ctgctctact atctggactt ggctgaagaa aaatctctac
     2761 gccagattga cctgccagat aaacttcatc tgagcccgag cgcagtttct aggatggtgg
     2821 ctcgcttgga agctaaaaat tgcggtctac tcagtcgcat gtgttgccat caagatagac
     2881 gctctagttt tatctgcctg acaaatgatg ggcaaaagac actggcctcg ctacaaaaga
     2941 ctgtcgaaga aagcttggaa gcaggtttgg atttcttgat ttaagatgat tttaaaaaaa
     3001 gttgcgtgcg caatcatttt tcttgacatt ctcttttata aggagtaaaa taaagttagc
     3061 attaaacaaa ggagttttaa atatgattga aattacctat atagatgcca gcaagaacga
     3121 aagaactgtg actttcgagt cttatgaaga ctttgaacgt tcacaacaag cttgccttat
     3181 cggcgtcgca gactactacc ctgtccaaaa attaacttac aagggtcata atttggacta
     3241 ccatgggact tatggagata tcttcttcta tctcatgaaa caagatttaa gccaatataa
     3301 ctaaaaaaag gagaaataca atggcaaaag caattacaga tgcaacattc gaacaagaaa
     3361 caaaagacgg tttggtctta gtagacttct gggcaacttg gtgtggtcca tgtcgtatgc
     3421 aaggtccaat cttggacaaa ttgtctgaag aactttcaga agatgtcttg aaaatcgtta
     3481 aaatggacgt tgatgaaaat ccaaacacag ctcgtgcttt tggaatcatg tctattccaa
     3541 ctcttctctt caaaaaagac ggccaagttg tcaaacaagt tgcaggtgtt cacacagcag
     3601 aacaaatcaa ggccatcatt gctgaattga gctaatcaaa cgagagacca agtactttct
     3661 ttggtctctt ttttcttgcc ctttgccatt tttcaaaaaa tatgctagac tatagtagat
     3721 tgaaact
//
LOCUS       DAVBCW010000136         3565 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVBCW010000136 DAVBCW010000000
VERSION     DAVBCW010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.136"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(84..1244)
                     /locus_tag="VQ514_001914"
     CDS             complement(84..1244)
                     /locus_tag="VQ514_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET2500637.1"
                     /translation="MKKKFALSFVALASVALLAACVEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTNMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            complement(1345..1593)
                     /locus_tag="VQ514_001915"
     CDS             complement(1345..1593)
                     /locus_tag="VQ514_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HET2500638.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            complement(1672..2262)
                     /gene="clpP"
                     /locus_tag="VQ514_001916"
     CDS             complement(1672..2262)
                     /gene="clpP"
                     /locus_tag="VQ514_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HET2500639.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            complement(2436..3065)
                     /gene="upp"
                     /locus_tag="VQ514_001917"
     CDS             complement(2436..3065)
                     /gene="upp"
                     /locus_tag="VQ514_001917"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HET2500640.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETFQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(3154..>3565)
                     /locus_tag="VQ514_001918"
     CDS             complement(3154..>3565)
                     /locus_tag="VQ514_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HET2500641.1"
                     /translation="ANRSTCKRAKVGAILVKDNKVISTGYNGSVSGTEHCIDHECLVI
                     EGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNCTKQLLQVGCKRVVYINQYR
                     MDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
BASE COUNT          982 a          762 c          664 g         1157 t
ORIGIN      
        1 agtttgaaga gattttcgaa gagtattaaa tatcgaaaca gggagtgagt caaaggctca
       61 ttccctattt caacattttt ctattatggt tttacaactt ctgctgcttc aactttacca
      121 ttgttcatgg tcatcatgta agcagttttg actgtgttgt ggtctgcatc gaagcttgtt
      181 tgaccagtta caccttcaaa atcttttgtt ttagcaaggt tatccttgat ttcacctgaa
      241 tttttagcac cttttgctgc gtttgctaca aggtgaactg aatcataagc caaggctgca
      301 aatgttgaag gctcttcatt gtacttagca cggtaagcgt caaggaaggc tttagcttta
      361 gctgaaactt ctacagtagt tgagaagcct gagataaagt agatgtttga tgctttttca
      421 gcagttgctt gttgtacaaa ctcctcaccg ttgaatccat caccaccaac gattggtttg
      481 tcaattccca tgccacgcgc ttggtttaca atcttaccag cctcattata gtaaccagga
      541 acaacgatag catcaaagtc tttccctttc atgtttgtaa gggctgcttg gaagtctgtg
      601 tcacctgcta cgaaagtttc atctgcaacg atttcaccct tgtatgactc gcggaaagat
      661 ttagcaatcc ctttagcata gtcactggca ttgtcagtgt aaagaacaac tttcttagca
      721 tttaattttt cagaaacata gtttgagata atttttcctt ggaagctatc ttggaaagtt
      781 ccaataaaga ggtaatcttg acctttagtc aatccatctt gagtcgcact tggtgagatc
      841 aatggaacac ctgcttttgt agcgttcgct accgcagctg cagtcgcacc agatgtcgca
      901 ggtcctacga ctgctgatac tttagattgg gttacaaggt tagttgtaac tgaagcagcc
      961 tcagctgttt cagacttatt atctttatcg actacttcga tttgttttcc atcgatacca
     1021 cctgcggcat tgatttcatc aacagccaat tgggcacctt tttgttcagc tgttccgtat
     1081 gcagctaaag aacctgattc ttcaaagtta aacccgattt taattgtctt ttcctctact
     1141 gagttaccag cagtgttgac tgctccagac ttcacttcta cacaggctgc aagaagtgct
     1201 acacttgcaa gcgccacaaa cgatagggca aattttttct tcatcttttt gtctccttat
     1261 ttcttaaata gtctgttaag aatataccga attatcagaa aattgtcaat gttttccttg
     1321 aactttttcg atgataacgt tttcctatcg ataaagattg cccacaaagg gtgtttccaa
     1381 ctcttggata tgacaaaccc taactttttt aataaatttt tccttgctca agcgtccgac
     1441 caattgctcc acttcttgag ttggaacata gagttgtaag taacgatgtt tcttggaatg
     1501 ataggtaata tctccataat cctgcagttt tttggcatca cgattatagt aaagatagat
     1561 aatcaagcca gagcgattga ctttttcaaa catgataaat cctctttcta agaaatctct
     1621 ccctattata ccaaaaaaag caaacccagt cgagtttgcc ttctatcatc attagttcaa
     1681 tgaattgttg gccataattt catcaataaa gccatattca agtgtttcct gggcgctcat
     1741 ccagttatca cgttctgcat ctgcatggac tttttccatt gactgacctg aattttcagc
     1801 caagattttt tccaaggtat tacgagtttt gagcaagtgt tctgcagcga tagccatatc
     1861 agtttgttgg gtaccaccac ctgtaccgcc cattggttgg tgaatcatgt attcagcatt
     1921 tggaagcatg aaacgtttgc cttttgctcc acttgatgcg atgactgttc ccatagatgc
     1981 agccattccc ataacaatgg tttggacatc tgccttgata aagttcatgg tatctacgat
     2041 tgccaaacca gctgaaacag aaccacctgg tgtattgaca taaaggtaaa tatcttttgt
     2101 actatcttgg gcatccaaga aaagcagttg ggcaataaca gagttagcca tattgtcttc
     2161 aaccggacct gtcagcataa tgatgcggtc tttgagaaga cgtgagtaaa tatcgtaaga
     2221 acgttctcca cggcttgttt gttcaataac tacaggaatc attcatttct ccttttgagt
     2281 tttaattttg ttggtcaaat gactgaagat aagactatta taatatcttg gtcaaaaaag
     2341 gtcaaatttt tgctctgctt tcattagaca gaaacaaaaa cccaacctcc tttcgtgact
     2401 ggaaatactt ttccaagtca ttcttctttt cgatcttatt ttgtaccgaa caagcggtct
     2461 ccagcatctc caagacctgg aacgatataa ccgtgttcgt tcaaacgttc atccaaggct
     2521 gctgtaaaga tttctacatc tggatgagct tcttgaaggg cttttacacc ctctggagca
     2581 gatacaaggc agacaaattt gatatttgat gcgccacgtt ttttaagaga atcaacagcc
     2641 aagattgctg agccacctgt tgccaacatt gggtctacta caaaaatttg acgttggtca
     2701 atgtcctcag gcaatttcac caagtattca actggttgaa atgtttcttc atcacggtac
     2761 ataccgatgt ggccaacttt agcagctgga accaagctca agagaccatc aaccatcccg
     2821 atacctgcac gcaagattgg gacgatggcc aatttcttac ctgccaattg tttttgaact
     2881 gtttttgtaa ttggtgtttc gatttccaca tcttctagtg gaagatcacg aagtacttca
     2941 taccccatca acattgcaat ctcatctact agctcacgaa aagcttttgt agaagtatct
     3001 gtacgacgca agattgacaa tttgtgttga atcagtgggt gattaataac ttcaattttt
     3061 cccatttttg gaattccttc tttcaattta ttcttcttat tataccaaaa aacgggttaa
     3121 aaatctttct aaaccattta tttttgataa tttttacatt agatcagcct ctttaagagc
     3181 tgtctgtact gtctcaagtg gtaaatgggt caattctgtc cctttttctt gataaaggta
     3241 ttgggcgtag tcgtccattc ggtactggtt gatataaacc acacgcttgc agccgacctg
     3301 aagcaattgt tttgtacagt tgagacaagg aaaatgggtt acataggctg taaagccttt
     3361 gggaacacca cgctcagcac cttgaaggat agcattgacc tcagcgtgaa gggtgcgaac
     3421 acagtggcct tcaatgacca aacattcgtg atcaatacaa tgctcagtcc ctgacaccga
     3481 accattgtaa ccagtggaaa taaccttatt atcttttacc agaatcgcgc ccactttagc
     3541 acgtttacaa gtggaacgat tcgca
//
LOCUS       DAVBCW010000137         3485 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVBCW010000137 DAVBCW010000000
VERSION     DAVBCW010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.137"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            174..632
                     /locus_tag="VQ514_001919"
     CDS             174..632
                     /locus_tag="VQ514_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HET2500642.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            645..1253
                     /locus_tag="VQ514_001920"
     CDS             645..1253
                     /locus_tag="VQ514_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500643.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            1324..1656
                     /locus_tag="VQ514_001921"
     CDS             1324..1656
                     /locus_tag="VQ514_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500644.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDYLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            1667..3082
                     /gene="ply"
                     /locus_tag="VQ514_001922"
     CDS             1667..3082
                     /gene="ply"
                     /locus_tag="VQ514_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HET2500645.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
BASE COUNT         1077 a          614 c          780 g         1014 t
ORIGIN      
        1 ggagcttaaa tcgtgtatca gttaattgtt tacttgcttc ataattcata gaactactat
       61 accatatttt gtttcgcagg aagtctaata agttatagtc cagtttttac tatactttga
      121 tattcaaata tgttatacta tgattatcaa agtaaatgaa aggagttacg aatatgacgc
      181 cagaagaaat gtacctgaca gagcgattag acgtacagat agctcatttt ttaaagaaaa
      241 gcgttcaaca tcgtaggcgc tataaggtat taaaaataac agaaatcgtg gcaggtttcc
      301 tcatagctgt cttttgtgct attcctatgc caggtgatcg ctaccgtttg atttcggttg
      361 ccttatccag tctcggcttg ctgtgtgagg ggattatcaa tttgtataat gcaaaggaaa
      421 attggatttc ttaccaaaaa actgcgcaac tcctggagaa agaaaaattc ctctatcaat
      481 gccaaacgga gaaatatgca ggaaagacca aggcttttgc cctatttgtc aagacatgcg
      541 aaggtcttat ctcagaggag attaaccagt gggaaagtat ccagtcaaaa gaagtggcag
      601 ctagtgcaga tgctccagtt aaaaaagagt aggaggtaga ggaaatgtct caatccagct
      661 acctgtcgcc cttgctctgg ttaaaaaaag aagccgataa ggaaaagatg agcgcgaccc
      721 agtgccagat atttttcttt tactatcaaa tgtttgagct cttatttgct agagaaagcg
      781 acatgaaaga cttatgtctg ggaacgaaag gtttttattt ctcgcagtta gagaaaaatt
      841 tgctttctgg agtttcccga tttctaaaaa acttggaggg gaaagtaact ctcaaggcta
      901 accaagaagt atcagctcgc aaagcccttt ttctagcctt gacaactagc caatcagatt
      961 ggcaggagtt agctcctgtt tttgattttt atcagactat cgggaggctt gaaaatcctt
     1021 ctctcttgag ttctcaggac agacaacatc tgatgtggat ttaccagtca gctttggaga
     1081 aggattatat tgtcaaggtt attggcgaca agcattttgt attgaagaga caagatgcta
     1141 ctaaattgac agcgcgccaa actcaaactt tggaaattct gagtcaatca gaagacttgg
     1201 tcaatcctgt ctatgttaca ttaggagaaa agggggtgct cttgcttgat taagagagga
     1261 gatgttgtag ctctttattt gccttttccg actattagta gcgatttggc tgtgaagaat
     1321 catatgtata tctgtattga caacagcatg actaaaaaca aagagttggt taaaaatcag
     1381 accttcaaac cagctctctt gaccagacgt ttggtcaaga actttatgat agaagagcca
     1441 gatctagctc gtaatccttt tacaagacca accttgattg acttagataa ggtatttatg
     1501 ttggataata cggtcattcc gacttcttat ctagccagac ggcgacgcaa tgtctcagaa
     1561 gaattgtacg aggaaatttt ggattactta gtccaaccac ggctgatttc gctgaacaag
     1621 tctgagttta tgcaactcaa tccaggaact tattaggagg tagaagatgg caaataaagc
     1681 agtaaatgac tttatactag ctatgaatta cgataaaaag aaactcttga cccatcaggg
     1741 agaaagtatt gaaaatcgtt tcatcaaaga gggtaatcag ctacccgatg agtttgttgt
     1801 tatcgaaaga aagaagcgga gcttgtcgac aaatacaagt gatatttctg taacagctac
     1861 caacgacagt cgcctctatc ctggagcact tctcgtagtg gatgagacct tgttagagaa
     1921 taatcccact cttcttgcgg tcgatcgtgc tccgatgact tatagtattg atttgcctgg
     1981 tttggcaagt agcgatagct ttctccaagt ggaagacccc agcaattcaa gtgttcgcgg
     2041 agcggtaaac gatttgttgg ctaagtggca tcaggattat ggtcaggtca ataatgtccc
     2101 agctagaatg cagtatgaaa aaatcacggc tcacagcatg gaacaactca aggtcaagtt
     2161 tggttctgac tttgaaaaga cagggaattc tcttgatatt gattttaact ctgtccattc
     2221 aggcgaaaag cagattcaga ttgttaattt taagcagatt tattatacag tcagcgtaga
     2281 cgctgttaaa aatccaggag atgtgtttca agatactgta acggtagagg atttaaaaca
     2341 gagaggaatt tctgcagagc gtcctttggt ctatatttcg agtgttgctt atgggcgcca
     2401 agtctatctc aagttggaaa ccacgagtaa gagtgatgaa gtagaggctg cttttgaagc
     2461 tttgataaaa ggagtcaagg tagctcctca gacagagtgg aagcagattt tggacaatac
     2521 agaagtgaag gcggttattt tagggggcga cccaagttcg ggtgcccgag ttgtaacagg
     2581 caaggtggat atggtagagg acttgattca agaaggcagt cgctttacag cagatcatcc
     2641 aggcttgccg atttcctata caacttcttt tttacgtgac aatgtagttg cgacctttca
     2701 aaatagtaca gattatgttg agactaaggt tacagcttac agaaacggag atttactgct
     2761 ggatcatagt ggtgcctatg ttgcccaata ttatattact tggaatgaat tatcctatga
     2821 tcatcaaggt aaggaagtct tgactcctaa ggcttgggac agaaatgggc aggatttaac
     2881 ggctcacttt accactagta ttcctttaaa agggaatgtt cgtaatctct ctgtcaaaat
     2941 tagagagtgt accgggcttg cctgggaatg gtggcgtacg gtttatgaaa aaaccgattt
     3001 gccactagtg cgtaagcgga cgatttctat ttggggaaca actctctatc ctcaggtaga
     3061 agataaggta gaaaatgact aggagaggag aatgcttgcg acaaaaagag gcgatgatct
     3121 ctctgcggat atctgaaagt ttatctcttg cctagcgatt tccattggag tatgctcgtg
     3181 atgttaaaat gaatctatca aacagaatgt gtaaaaaagt aaaaagggta gccaattcct
     3241 tgatatacag gggattgatt atcctttact tgttttagat gattggaaat tcaagaacga
     3301 cacgtatcgg aaatattttg ctgtttatat ctaataccca tttatggaag aaatgaaatt
     3361 aaatatgaac aaatcaattg ctagcaatgg tttagaagtg ccagtgtact agtctaactt
     3421 ctccatcttg ggaacacgta aattatactc ttcgaaaatc tcttcaaacc aggtcagccc
     3481 tatcc
//
LOCUS       DAVBCW010000138         3479 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVBCW010000138 DAVBCW010000000
VERSION     DAVBCW010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.138"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            65..1305
                     /locus_tag="VQ514_001923"
     CDS             join(65..404,404..1305)
                     /locus_tag="VQ514_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET2500646.1"
                     /translation="MVKLIDRYGIEIVKKGKNRHYSSKLKQEMMDKALLEGCSQRSIS
                     LDYALPNQGMLSFWPAQYKKNGYTIVEKTRGRPAKMGRKRKKTWEEMTELERLQEENE
                     RLRTEVAYLKKFKRVRGKGRSPRARKAETVREMVSGGFRLDFLLETARLARSTYYYQL
                     KQLDGVDKDKEIKTEIQGIDNEHKGNYGYRRIHLELRNRGFVVNHKKVQRLMRILGLT
                     ARIRRKRKYSSYQGEIGKKAENLIQRQFEASRPMEKCYTDVTEFAIPNSTQKLYLSPV
                     LDGFNSEIIAYHLSTSPNLEQVKSMLEQAFTEKYYENTILHSDQGWQYQHDSYHRFLE
                     SKGIQASMSRKGNSPDNGMMESFFGILKSEMFYGYEKNFRSLENLEQAIVDYIDYYNN
                     KRIKVKLKGLSSVQYRTKSFG"
     gene            complement(1540..2148)
                     /locus_tag="VQ514_001924"
     CDS             complement(1540..2148)
                     /locus_tag="VQ514_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HET2500647.1"
                     /translation="MQIKFIDKVSNLIMLNLLYVASVVTVIAIGSGESALIATLIKIV
                     RHEESYPYRDFANSFFKDYWKNLGAALISNLPILILLFSLFFLPYIPLPIYIISILRH
                     IGVIYIILHLIATTFLIPLIGRYNNTLKNSLHNSIMLAYKHFFIAVLIRIIEIIPVLL
                     FFILQNQLLVWITLMIFILPSITKYANAFLYNFIFSKYEKLN"
     gene            2314..2484
                     /locus_tag="VQ514_001925"
                     /pseudo
     CDS             2314..2484
                     /locus_tag="VQ514_001925"
                     /inference="COORDINATES: protein motif:HMM:NF013577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
     gene            2473..3084
                     /locus_tag="VQ514_001926"
     CDS             2473..3084
                     /locus_tag="VQ514_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001821036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HET2500648.1"
                     /translation="MTKDILELESQKMSSDTFIDEIKNNYLSIVESTRKLIDGRQIEL
                     AIKLIREANQILMIGVGSSGNAAREFESSLLRIGIISKTVIDTHFQLMHTALLKDNDL
                     IIAFSLSGSTKEVEETLLNAKRKNVKIISITNYSSRNIAKLSDCVLLTSKKESYLEGG
                     SLMAKASQLFIIDVICTRLSLINYEDTICKKEEIASLLSNKVE"
BASE COUNT         1315 a          443 c          620 g         1101 t
ORIGIN      
        1 gcaaggacaa agcttcaaac agctttcaaa aagatttggt gtggatgttt ctggtctaaa
       61 gtacatggtg aaattaattg accgttatgg catagaaatc gttaaaaaag ggaagaatcg
      121 tcactattct tctaaattga aacaagaaat gatggataaa gccttactgg aaggttgttc
      181 acaaagaagt attagtcttg attatgccct cccaaaccaa ggaatgcttt cattttggcc
      241 ggcacaatac aagaaaaacg ggtatactat tgttgagaaa acaagaggga gaccagctaa
      301 aatgggacgt aaacggaaga agacttggga agaaatgaca gaactagaac gactccagga
      361 ggagaatgaa cgcttacgga ctgaggtggc ctacctaaaa aagttaaaag agttagagga
      421 aagggacgaa gccctagagc gagaaaggca gagacagtta gagaaatggt ttcaggagga
      481 tttcgactag attttcttct tgaaacagca cgtttagctc gctcgactta ctattatcag
      541 ttgaaacaac tggatggggt tgataaagat aaagagatta aaaccgaaat tcagggcatt
      601 gataatgaac ataaaggcaa ctatggctat cgtcgaatcc atttagaact aaggaatcgt
      661 ggttttgtgg tcaatcataa gaaagttcaa cggttgatga gaattcttgg tttaacggct
      721 cgaattcgcc gtaaacggaa gtattcttcc taccaaggag agattggcaa gaaagcagag
      781 aatctcattc aacgccagtt cgaagcatcg aggccaatgg aaaagtgcta tacagatgtg
      841 acagagtttg ccattccaaa tagcacacag aaactctatt tatcaccagt tttagatggt
      901 tttaacagtg aaatcattgc ttatcatctt tctacttcgc caaacttaga acaagtgaag
      961 agtatgttag aacaggcctt tacagagaaa tactatgaga atacgattct ccatagtgac
     1021 caaggctggc aataccaaca cgattcttat catcggttcc tagagagtaa gggaattcaa
     1081 gcatccatgt cacgcaaggg caacagccca gacaacggca tgatggaatc tttctttggc
     1141 attttaaaat ccgaaatgtt ttatggctat gagaagaact ttagatcttt agaaaacctt
     1201 gaacaagcta ttgtggacta cattgattac tacaacaaca aacgaattaa ggtaaaacta
     1261 aaaggactta gttctgtgca gtacagaact aaatcctttg gataaattat ttgtctaatt
     1321 ttttggggtc agtacagtat taaataccat gatcttatgc gttttataat aaaaaatctg
     1381 ttcttgtttt atttttagaa aatttactaa agactaaaaa tattatgaat aatttaaaaa
     1441 atagtcaggt tatatagttt tttgaaatta aaattatttt atcgttgaaa ttagttaaaa
     1501 atttttataa aatcaagatg caaggctatg agatgttctc tagtttaatt tttcatactt
     1561 cgagaatata aagttatata aaaatgcatt agcatatttt gttattgagg gaagtataaa
     1621 aatcattaaa gttatccaaa ctagtaattg attctgtaat ataaagaaga gtagaactgg
     1681 gataatttca ataattctaa taagaacggc aataaaaaaa tgtttatagg ctaacatgat
     1741 tgaattgtgt agagaatttt ttaaagtgtt attatatcta ccgattaaag gaattagaaa
     1801 tgttgttgct attaaatgaa gtatgatata tattactcca atatgtcgta agatagaaat
     1861 gatatatata ggtaatggta tgtagggtag gaagaacaaa ctgaataata agattaaaat
     1921 tggtaaatta gatattaatg ctgctcctaa atttttccaa taatctttaa aaaaactgtt
     1981 tgcgaagtct ctatagggat aggattcctc gtgtcgtaca atttttatta gagttgctat
     2041 tagggcactt tcacctgatc cgatagcaat aactgttact actgacgcta catagagtag
     2101 atttaacatt ataagatttg aaactttatc aataaatttt atctgcatct ttaactcctg
     2161 ctgtttttat acttataata tcacatcatt tgagtttcat caactttgaa tacattaaga
     2221 aagaatataa ctatgaggaa aggaaaaaat tattatgaat aaatccaaaa aatataaaga
     2281 aagaattcat tatgaatacg ataatcttac taaatcagag aaaaaattgc tgactatatt
     2341 ttagttagag atagtgaaat agtttatgaa acgtcttcag aaattgttag taaagtaaaa
     2401 gttggagagg gaactcttaa tcgattttgc aaaaagattg gatactctgg ttttcaggaa
     2461 ctaaaattaa aaatgacgaa agatatatta gagttagaat ctcaaaaaat gagtagtgac
     2521 acatttattg atgaaataaa aaataattat ttatctattg tggaaagtac aagaaaatta
     2581 attgatggta gacaaataga attagctata aaattaataa gagaagcaaa ccagatttta
     2641 atgattggtg tgggttctag tggaaatgcg gctagagaat ttgagagtag tttgttaaga
     2701 attggaataa taagtaagac ggtaattgat acccattttc aactcatgca tactgcgtta
     2761 ttgaaagata atgatttgat aatagcgttt agtttatctg gttccacaaa agaagttgag
     2821 gaaactttat taaacgcaaa aagaaaaaat gttaaaatta tttcaataac aaattattcg
     2881 tccagaaata tagcaaaatt atctgactgc gttcttttga catcaaaaaa agaatcttat
     2941 ttagagggtg gtagtttaat ggctaaggct tctcaactat tcataattga tgtaatatgc
     3001 actagactct ctttaataaa ttatgaagat actatatgta aaaaagaaga gattgcttct
     3061 ttgctgtcaa ataaagtgga ataaatcaat aatttttaaa ttttaataaa aattttttaa
     3121 aataaattaa aaaacgatcc taaaagggtc gtttttttgc taaagtttta aaaataaaca
     3181 ataattaaga ttatagtgcg ttgaatctat attaggacat aacaattctt aaatcattgt
     3241 tagaaattag tttgaatttt ccaatcaatt tgttcgattt ttacatcatt gcattatatc
     3301 acgtgactat taaccttgtt ttctcctaaa attgattttc ttcttttctt atcttgtcca
     3361 ctcgaaacaa gtattgtaag aatttgatta tttttgaaag tacttttaat atatttgaga
     3421 tagttaaaaa agatttgaaa ctaaattcca aattagaaaa agacttgaaa tgcttaaaa
//
LOCUS       DAVBCW010000139         3405 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVBCW010000139 DAVBCW010000000
VERSION     DAVBCW010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.139"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            139..2772
                     /gene="polA"
                     /locus_tag="VQ514_001927"
     CDS             139..2772
                     /gene="polA"
                     /locus_tag="VQ514_001927"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HET2500649.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVAEGLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIVVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYMQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            2857..3294
                     /locus_tag="VQ514_001928"
     CDS             2857..3294
                     /locus_tag="VQ514_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET2500650.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
BASE COUNT          921 a          594 c          872 g         1018 t
ORIGIN      
        1 tactatatta tagcacattt ttaaatctag gtttactaaa ttctctggct ctatctattt
       61 attcggaaaa agtttgaaaa atacttgctc tagctcttcc caatggtatt ttttgattct
      121 ttcctttata atgggtgtat ggataagaaa aaattattat tgattgatgg gtcttctgta
      181 gcttttcggg cgttttttgc gctgtatcag cagttggacc gttttaagaa tgcggctggt
      241 ttgcatacca atgcgattta tggttttcag ttgatgttga gtcatttgtt ggagcgggtt
      301 gagccgagtc atattttggt ggcttttgat gcgggaaaga cgaccttccg gacagagatg
      361 tatgcggact ataagggtgg tcgggccaag actcctgatg agtttcgtga gcaatttcct
      421 ttcattcgtg agttgctgga tcatatgggg attcgtcact atgagctggc tcagtatgag
      481 gcggatgaca tcattgggac gttggataag ctagcagagc aggatggttt tgatattacc
      541 attgtcagtg gggacaagga tttgattcag ctgacggatg agcatacggt ggttgaaatt
      601 tccaagaaag gtgtggctga gtttgaggcc tttacgccag attacctcat ggaagaaatg
      661 ggcctcacac cagctcagtt tatcgatctc aaggcgctca tgggtgataa gtcggataat
      721 atccctgggg tgaccaaagt cggtgaaaag acgggtatta agctcttgct ggagcatggt
      781 tcgcttgagg ggatttatga aaatattgat ggaatgaaga cttctaagat gaaggaaaat
      841 ctcatcaatg acaaggaaca ggcctttttg tctaaaacac tagcgaccat tgataccaag
      901 gcaccgattg cgattggttt agaggacttg gtctatagtg gtccagatgt tgaaaatctt
      961 gggaaatttt acgatgagat gggattcaaa cagctcaagc aggctttaaa tgtgtcgtca
     1021 gctgatgtgg ctgagggttt ggattttact attgttgacc aaatcagtca agatatgctg
     1081 agtgaagagt ctatcttcca ctttgagctt tttggtgaga attatcatac ggataatttg
     1141 gttggatttg cctggtcttg tggggataag ctctatgcca cagacaagct tgagctgttg
     1201 caagacccga ttttcaagga tttcttagaa aaaacatctc tgagagttta tgactttaag
     1261 aaggttaaag ttcttttgca acgttttggt gtggatttgc aggcgcctgc ttttgacatc
     1321 cgtttggcta aatacctcct ttcgactgtg gaggacaatg aaattgcgac catcgctagt
     1381 ctttatggtc agacttactt ggttgatgat gaaactttct acggtaaggg tgttaaaaag
     1441 gccattcctg aacgtgagaa attcttggaa cacttagctt gtaaacttgc tgttttggta
     1501 gaaacagagc ctattttact tgaaaaactc agcgaaaatg ggcaattaga gcttctttat
     1561 gatatggagc aacctctggc ttttgttctt gccaagatgg aaattgctgg gattgtggtc
     1621 aagaaagaga ccttgcttga gatgcaggct gaaaatgagc ttgtcattga aaaactgact
     1681 caagagattt acgagctggc tggtgaggag tttaatgtca actcgcctaa gcagttgggc
     1741 gtgcttctct ttgagaaatt gggacttcct ctagaataca ctaagaaaac caagacaggt
     1801 tattcgacag cagtggatgt gttggagcgt ctcgctccta ttgctccgat tgttaagaaa
     1861 atcctggatt accgtcaaat tgctaagatt caatctactt atgtaattgg cttgcaggac
     1921 tggattttgg ctgatggaaa gattcatact cgctatatgc aggatttgac ccagaccggg
     1981 cgtttgtcta gtgtggatcc aaacttgcaa aatattcctg cccgattgga acaagggcgc
     2041 ttgattcgga aggcttttgt gccagagtgg gaggatagtg tgctactcag ctctgactat
     2101 tcacagattg aattgcgcgt tttggcgcat atttctaagg atgagcactt gattaaggcc
     2161 ttccaagagg gggcagatat ccatacttcg acagccatgc gggtctttgg cattgagcgt
     2221 cctgataatg tgactgcaaa tgatcgtcgc aatgccaagg cagttaactt tggagtggtt
     2281 tatgggattt cagactttgg cttgtctaat aatttgggaa ttagtcgtaa ggaagccaaa
     2341 gcctacattg atacctactt tgaacgtttt ccaggtatta aaaactacat ggatgaagtg
     2401 gtgcgggagg cgcgtgataa gggctatgta gagaccctct ttaagcgtcg ccgtgagttg
     2461 ccagatatca attcgcgcaa cttcaatatt cgtggttttg cggagcgaac tgctatcaac
     2521 tcacctatcc agggttcggc agcagatatt ctcaagattg ccatgattca gctggataaa
     2581 gccttggttg caggtggtta tcagactaag atgctgttac aagtgcacga tgaaatcgtc
     2641 cttgaagtgc ctaaatctga attggtagag atgaaaaaat tggtgaaaca aaccatggaa
     2701 gaagccattc aactcagtgt tcctcttatc gcagatgaga atgaaggggc aacctggtac
     2761 gaggctaaat aaaaaggggg ctagtcctcc ttttttgtag tagaattctg caaacctttt
     2821 caaaatgtgc tatactgatg aaaaaggagg ttttctatga gtcaagaatt tatcaatcca
     2881 agtgatggcg tgattcgtca gtatctcgca acgagtaaaa cccttgctgt ggtgggtttg
     2941 tctgaccgtg aagaaacaac cagcaatcga gtgacaaagg aaatgcaggc tcggggctat
     3001 aaaatcatcc cagtcaatcc caaggcggca ggtggcgaaa tcttgggtga aaaggcttat
     3061 gctagcctcg ctgaaattcc ttttcctgta gatattgtca atgtttatcg tcgcagtgaa
     3121 tttctgcccg atgtggcgcg tgattttctc aaagctgatg ccaagatttt ttgggcacag
     3181 ctaggacttg aaagtttaga agcgaaagag attttgcgtg atggtggatg tgatgatatc
     3241 gtgatgaatc gctgtatcaa gagagaacat actcgcttga ttgaggaagc ataagaaaaa
     3301 ggtagctgat gggctacctt ttgtgttata ctcaatgaaa atcaaagagc aaactaggaa
     3361 actagtcgcg ggctgctcaa aacactgttt tgaggttgta gatac
//
LOCUS       DAVBCW010000140         3398 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVBCW010000140 DAVBCW010000000
VERSION     DAVBCW010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.140"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            549..1178
                     /locus_tag="VQ514_001929"
     CDS             549..1178
                     /locus_tag="VQ514_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET2500651.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            complement(1301..3193)
                     /gene="pepO"
                     /locus_tag="VQ514_001930"
     CDS             complement(1301..3193)
                     /gene="pepO"
                     /locus_tag="VQ514_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HET2500652.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
BASE COUNT          935 a          787 c          656 g         1020 t
ORIGIN      
        1 aaaatcctca agatactttc ttctatcctt tagtttataa ggagaatacc tatgaaaaaa
       61 ctgctatttc tatctttgct ctcctaatgt taggagtttg ctgcctgttc ctattcagcc
      121 agcaaagcta taaaaaactc gttcaatact atgctaacga ccagaacctg cccagtagga
      181 taacttatag tgaatatagc gacaaatgag aagccaacta cggtagcact ctaaacatca
      241 cgtctatcaa acaagctaat gacggagttt atgcaaccta tgaagggcaa ttgacacctt
      301 tccaatattg ataaattgat aaccagcctg tcttcatcta gtcatgctgg tttttaagtt
      361 cattttaaat ccttacctat tctccctaac tgtgctatac ttaatttata ctcaatgaaa
      421 atcaaagagc aaactagaaa gctagccgca ggctgttcaa agcactgctt tgaggttgca
      481 gataaagttg acgcggtttg aagagatttt cgaagagtat tagtacattc tttgagattg
      541 gagttagtat gaaaatccat aaaaccgtga atcctgttgc ctatgaaaat acctattatc
      601 tagaaggcga aaagcacctc atcgtcgtcg atcctggtag tcattgggaa gccattcgtc
      661 agacaatcga gaagatcaac aaaccgatct gtgctattct cttgacccac gcccattatg
      721 accatatcat gagtctggac ttggttcgcg agacgtttgg caatcctcct gtctatatcg
      781 cagagagcga agccagctgg ctctacactc ctgtcgataa tctctccggt ctccctcgcc
      841 acgatgatat ggcagatgtg gtcacaaaac ctgcagaaca cacctttgtc tttcacgaag
      901 aataccaact agaggaattt cgttttaagg ttctaccgac cccagggcac tctatcggtg
      961 gtgtttccct agtctttcct gatgctcacc tagtcttgac gggagatgct ctattccgcg
     1021 aaactatcgg acggaccgac cttccgactg gtagcatgga gcaactcctt catagtatcc
     1081 agacccaact cttcacccta ccaaactacg atgtctatcc aggacatggt ccagctacta
     1141 ctatcgctca cgaaaaggcc ttcaatccct ttttctagca agatgatgac aatcgaaatt
     1201 taagtaaact atccagcaaa tctttctatt acaaaaggca tcctatcaag gttttcacac
     1261 gtgattggat gccttttttc tgatgactag attttttgta ttaccaaata atcacgcgct
     1321 cctctggtga acgccacatt ccgtctcctt ctttgacatc ataggttgta aagaaatcgt
     1381 cgaagtttgg tacttgcaca ttgacacgga gtttggctgg tgcgtgcaca tcgacgctag
     1441 ccaaaagttt cataaattct ggtcgacctt tcatgcgcca gatgcgaccg aagttgtaga
     1501 agaactcttc tgctgagaag tctgcttctc tcttagctgc ttcaagcgct gctgcgattc
     1561 ctcccaagtc agccacgttt tctgatacag tcaatttacc gttaatggtt gctccataag
     1621 aatcctgtcc atcaaattgg tcaatgactt tttgtgtttt ctccttgaag gcagcatagt
     1681 cgctctctgt ccaccaatcc ttgaggctac cattttcgtc aaaggaagcc ccgttagtat
     1741 caaaggcgtg ggaaatttca tgggcaatca ctgccccaat accaccgtag ttagcagaag
     1801 atgactgatg caagtcatag aaaggcgcct gtaaaatggc cgctggaaag acaatcaggt
     1861 tcttctgagg attgtagtag gcattgacca tatgagcagg catgccccat tccttatagt
     1921 ctacaggctg gttccactta ctccaactgt gcttgatttc cacacgcgca aaggctagag
     1981 cattctcaaa aagactggca gtttcattca ctaccttatc cttgtaacgt gcaggcaatt
     2041 cttctggata gccaatataa ggtttgatca cattgagctt cacgatagcc tgtttacagg
     2101 tttctggagt gagccagtca ttcttaagca gacgctcctt atagacatca atcatggttg
     2161 ccactttttt ctccacatcc gccttggctt ctggagagaa cttctcacgg gcgtaccaaa
     2221 gccccagggc ttgcttgaaa ggttcctgtg ctagatgata agctgctttg accttatctt
     2281 ttgcctctgg aactccagaa agggcacggc tgtaggcacc agacaaaaca cggatatcct
     2341 ctgttaaata gctggttgaa agattgacaa cactcaaaat caaggttgct ttaaggagag
     2401 accaggcttc ctcactgtaa aattgctctg ctgcttgcca gaaacgttcc tcgtctacaa
     2461 taaccttgtc tggtaattgc ccaataactg ctttgaagaa gtcatccaaa ggtagggcag
     2521 gcgcgaattt cttgaaatct tcgtaagaat atggatgata gagtttagca tattctgaac
     2581 tttcttcatt agagagcacc actgccgcaa ctcggcggtc caattcaagt cttttttcta
     2641 gcaagtcttc aatttcttca tcagagaaat cataagcctt gaggagattt gcgctgcttt
     2701 ctttccaaag agtcaagagc tcttcgcgct gaggatgttc ttctgcatag taggtcgtat
     2761 ctggcaagat tgtgcttgga gcgctagccc atagaacatt gattctagca tccataaagt
     2821 ctggcgatac accaaaagga aggaagtttg gttttcctgc aagctcaaac tctgctagtt
     2881 tagctgtaaa atccgcaaaa gtttccaatt cttggaattc tttaaggagt ggtaagacag
     2941 gtgtgatacc gtcagcttct ctcttgtcaa aatcacgaac taggcggtgg tatttgacaa
     3001 agttttccaa gatagcatcc tcaggcactt cttcacctgc taaccacttg tctgttgtcg
     3061 ccagcatcag gtcttcaatt tcctggtcta aatcaacaaa acctcctgtt tgagacttat
     3121 ctgctgggat ttcagctgtc tgttgccatt ctccattgat agcatcataa aaatcatctt
     3181 gataacgtgt catcttgttc tcgctttcat ttgtatttgc atttatctta acaaaaatca
     3241 ctagggaatg caattaataa ccatttaact attatttttt agctattata aaaaaccttt
     3301 ccatgataaa acgcataatt ccaagttttt tacacttgat actatgcgtt ttataattta
     3361 gaaatttatc ctgaaagtta tctttagaat ctattttt
//
LOCUS       DAVBCW010000141         3262 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVBCW010000141 DAVBCW010000000
VERSION     DAVBCW010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.141"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(216..827)
                     /gene="rpsD"
                     /locus_tag="VQ514_001931"
     CDS             complement(216..827)
                     /gene="rpsD"
                     /locus_tag="VQ514_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HET2500653.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(1022..2074)
                     /locus_tag="VQ514_001932"
     CDS             complement(1022..2074)
                     /locus_tag="VQ514_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HET2500654.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVTKLEKGQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(2071..2769)
                     /locus_tag="VQ514_001933"
     CDS             complement(2071..2769)
                     /locus_tag="VQ514_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2500655.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(2936..>3262)
                     /locus_tag="VQ514_001934"
     CDS             complement(2936..>3262)
                     /locus_tag="VQ514_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET2500656.1"
                     /translation="VKETSDTANGSLSPSNSGSGVTPMNHNHATGTTDSMPADTMTSS
                     TNTMAGENMAASANKMSDTMMSEDKAMLPNTGETQTSMASIGFLGLALAGLLGGLGLK
                     NKKEEN"
BASE COUNT          912 a          736 c          625 g          989 t
ORIGIN      
        1 ttttttacga aaatctattg aatatgccat aaaaggatta taccacattg tgtactattt
       61 ttggttcatt ttactatatc actttggact atatcggtct agttatgtct aaacgattat
      121 agctggatac taagagaaat caaaaaagca ctaagggaga acgtcccaaa gtgcttaata
      181 tcaaggcctc aaagcctgtc ttattcaata aaatattaca acatcttgtt gtagaattca
      241 acgacaagtg cttcgttgat ttctgggttg atttcgtcgc gttctggcaa gcgagtcaat
      301 gaaccttcca atttttcagc gtcgaatgat acgaatgctg gacgtccaag agtagcttct
      361 actgcttcaa ggattgctgg aactttcaat gatttttcac gaactgagat cacttgacct
      421 ggagttacgc ggtatgatgg gatatcaacg cgtttcccgt caacaaggat gtgaccgtgg
      481 tttacgaatt gacgagcttg acgacgagta gtcgcgagac caagacggta aacaacgtta
      541 tccaaacgac gttccaaaag aagcataaag ttgaaaccta ggattccgcc tttgattttt
      601 gtagcttgta cgaacaagtt acggaattgt ttttcaccta caccgtaagt gaaacgaagt
      661 ttttgttttt cagccaattg caaaccgtat tctgacaatt tagaacggtt gtttggtccg
      721 tgttgtcctg gtacgtagtt acgacgtgcc aattctttac ctgtacctgt aagtgaaagg
      781 ccaaggcgac gagcttgttt ccaagatggt cctgtataac gtgacatatg aatgtcctcc
      841 tgatataaat aatatttcgg tggaaatagt cacttagaaa gccctgattc gtgcagatgc
      901 ccttcgccta aacagccaag gttacttgtc ataagacacc tgttgacgag cttcatgctt
      961 tcctgctgat atttcacaca aaatccattt taccatgaat agctggattt gtaaaggggt
     1021 tttaggcttt attttcacta ccagagagat tgagaacgag ggtaaaggta cttcctagac
     1081 cgtactggct gctgactgtg atttccccac ccaattgatg ggccaattca cgcgcaatcg
     1141 caagtcctaa tccatgacca cctgtcttca tgttacgcga agtttcgaca cgataaaggc
     1201 gtttgaaaat attttccaaa tcctctgggg caatgccctg cccttcatcg gtcacactga
     1261 ttgaaagctg gcccttctcc agcttagtca ccacttccag cttggttcct ggagcagaat
     1321 atttaaaagc gttatcgacc agattcacca agatacgaga aagcttagca taatctccct
     1381 caatccgggc agactctggg attacctgca agtggacatc tcttctctcc tgctcaatca
     1441 aaaactgaaa ttcactcatg cactcaatta agagcttgtc cagaaaaata ctgtctttac
     1501 tggtagtttc cacctgattt ctagctgtgt ttagggtcaa aaaattcaac tcctcaacca
     1561 gtttattgag cctctccgtc tggcgtccaa tggttgctag ataatgagct tgctccgact
     1621 ccttgataat cccatccaaa atcccttcta ccgtcgcttg gatcgaagtg ataggggtct
     1681 taatatcatg cgacaactgg gcaatcatca agcccttttc tcgttcgctt tcttccaagg
     1741 aatcaaagct tacctgcaaa tcatgggaca tctcattaaa agtttgccct aattgctgaa
     1801 attctacagg accttgaacc tccaaatttg aaggaaaatc cttggccgct acccgcttgg
     1861 catgctcctt gagtttgccc aacgacgtaa agactggcaa taggagaaag agactaatcc
     1921 cagcaccgac aaggctggca acgatggtca tcccaagcaa aaagtaaatc tcgcctttcg
     1981 caatcagcat tttttgaaca gcccaaaaaa caaccaaaat ggttaagagg gttgaaataa
     2041 tatatccaac caaaatataa ctttttagtt tcatgtttgt cctctcggtt tctctatctt
     2101 atatcccaac ccccaaactg tcttaatagt gggcgtttgg tcactactat attttgccag
     2161 ctcctgtcga agagcatgga tatgcacatt caaggtattg gtgtcatcca cgtagtcttc
     2221 tttccagatc ttttcataga ggtctgtctt ggagaaaact cgctctggat tgctagctaa
     2281 aatccacagc aattcaaatg atttaacagt taaatccagc atttcttctc ctatttgaac
     2341 ttcatgacta ctatgattca tttttagatt gccaagggaa atcagctctg tttcgccccc
     2401 acgatgaagg cggcgcaaaa tattgtggac acgcaaaacc agctcacgtg ggctaaaagg
     2461 cttagcaata aaatcatctg ctcccaagct caggccgtaa atcttgtcct gttcactggt
     2521 cttagcagta ataaatagga aaggctgctc tggtgataag tattgaacct cactgattaa
     2581 atcataacca tccatccgag gcatcataac atctgtgata atcaaatcaa tcggtttttt
     2641 cttgaacagc tctagcgctt ccagtccatc atgggctacc aagacctgat aacctgcctg
     2701 aattaagtat ctctgatgaa tatctgtgat ttctacctcg tcgtcaacga gtaaaattgt
     2761 ctttcccatc tgtctctcct ttgataaaaa cagtgctata ctgctttaag tataacacta
     2821 tttttattgt tttacgatga ttttaatctg ctattgaaag aaactcctga ttgctgattg
     2881 agttgttaaa ctaatcttag tcagcccact atccatcatt tatttcctta gctgattagt
     2941 tttcttcttt tttgtttttc aaacctagac caccgagtaa acctgcaagc gcaagcccaa
     3001 ggaaaccaat acttgccatt gatgtttgag tctcaccagt atttggtagc atagctttat
     3061 cctctgacat catcgtatca gacatcttgt tagcagaagc agccatgttt tcacctgcca
     3121 tcgtgttggt agaacttgtc atggtgtcag caggcatgct atctgtagta cctgtagcat
     3181 gattgtgatt catcggagtc acgccagaac cagagttaga aggtgataat gaaccatttg
     3241 ctgtgtctga tgtttcttta ac
//
LOCUS       DAVBCW010000142         3219 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.142, whole genome shotgun sequence.
ACCESSION   DAVBCW010000142 DAVBCW010000000
VERSION     DAVBCW010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.142"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(19..726)
                     /locus_tag="VQ514_001935"
     CDS             complement(19..726)
                     /locus_tag="VQ514_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2500657.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVAGLSIVVLALFIMGARKTKLASFNFSFFRAKDLARLVLSYLVIICSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            complement(779..2197)
                     /locus_tag="VQ514_001936"
     CDS             complement(779..2197)
                     /locus_tag="VQ514_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HET2500658.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLTMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNDENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHAMIWILDALFILNYISMAL"
     gene            2387..3199
                     /locus_tag="VQ514_001937"
     CDS             2387..3199
                     /locus_tag="VQ514_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2500659.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKKTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKEMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
BASE COUNT         1097 a          700 c          579 g          843 t
ORIGIN      
        1 gttcctacat aaaaactttt acacagaaat tcctaatgtc cgactcataa tcaccacaag
       61 agccaacaaa cagaaagcaa tcccattaac aatcatgtga agcaagatcg acatttccaa
      121 acgttgggtc ttgtaggccg tccaagatag aactgtcgac atacctccat aaatcaataa
      181 agaaggtaaa ttacttggtt gatgcaataa agcaaacaca atcgtaccga ctacaaatcc
      241 caagttctcc ttgcctcgga aaatcttttt aggaacaatc ccacggcaca agatttcctc
      301 acaaattgga gcaagcaagg ctagcaagaa gaagctagaa atcaacgaac tattctgaac
      361 catatcgtta atctgagact ggttagctgt tgtcgtctca tttgacagtt gcaacaagat
      421 agaaccaagt atatttgaac agataatgac taaataactc aagaccaaac gtgccaaatc
      481 tttagctcta aaaaaagaaa aattaaaact agctaacttg gttttacgag ctcccataat
      541 aaatagagcc agaaccacaa ttgaaaggcc agcaactatc agccctgact gtagcagggc
      601 tacttctttt aaagtcaaaa tagaggtaac cactagggga acctgatata aaaggacggc
      661 aagtaaaaag accaaaatcc agcctgcacg attcaacaat tctttccaca tatttttctc
      721 tttcatcacc tatctccttt gtattaaaaa aagggggaaa tccccctggg tcattctatt
      781 ataaggccat gctgatgtag ttaaggataa acaaggcatc caaaatccaa atcatggcat
      841 gaacatcttt aacttgacct ttgacaagct tagtcaaagt gtaagtcaag aaaccaactg
      901 caatcccttg agtgatagag tagctgaatc ccataaagat agatgtgaag aaggcaggaa
      961 ctgcttcaga catatcatcc caatggatat ttttcaagct accaagcatc ataatcccaa
     1021 cgataatcaa gattggagct gtagccgctg ttggtacgat cgctagaagt gggctaaaga
     1081 agcttgaaat cgcaaaacag atagctacaa ccaaggctgt caaaccagta cgtccacctg
     1141 caccgatacc agcagcagac tcaacataag tcgttacgtt tgaagtacct gcaatggcac
     1201 cgactgtcgt tccaatcaaa tcagagtaaa gagccttatc caatttggct gattggtgat
     1261 tttcatcatt tgtcgctaca ataccaactt tttcacctgt accgatcaag gtaccaattg
     1321 tgtcaaaaat atctgtcaat gagaaggcaa gaatggccat cagagtttca ggcaagcgag
     1381 ctgtatctga aaccaaagct cccaaacctt ctgaaccaag agctgcacca aagattgtct
     1441 tcaaatcttc aaaagctgca ccaacatggt tattagcaaa atcgatacta gacaaatcaa
     1501 ccaaaccaac tgcaatagca agaacagttg ttgtcaagat agagagaata attccccctt
     1561 taatcccttt gatgacaaag aagatagtaa tggcaagtcc tgcaagagcc actaaaacag
     1621 ctggattatt aaagctgacc aatcctggaa ctgctgaaga gtttgctgca atcgttgctt
     1681 gagctttgtc agccccttct cctacaacag tatagttgcc tggatcaatc gtgaatttca
     1741 aaagtccagc attcttaatc cctacatagg caaggaagac accgatacca gctgaaatag
     1801 ctgagcgaag agcattggga atcgattcaa tgatcatttt acgaacattt gtcaaggtaa
     1861 taatcaatga aataatccca cagatgaaga ccatagctag ggcttcttgc caagaataac
     1921 caagtccaaa tacgactgta aaggtaaaga aggcattgag tcccatacct ggtgcttgag
     1981 cgtatggcaa gttagcatag aaggccatcg tcaaggtacc agcaactgca ccgataatcg
     2041 ttgcaagaaa cacaccctga gcaggcattc ctgtttgtga aagtatttgt gggtttacaa
     2101 agagaatata gctcattgca aagaaagttg ttaaaccagc gagaacctct gtacgaacgt
     2161 ctgtaccgtt ctcttttagt ttaaataatt tgtccatcat caatctcctt ttaattttta
     2221 cgaacaataa tagaattata tcatttatca ttcacttttt caatgtcttt gtttgatttg
     2281 tttaagaaat taatccaatt tcattttttg tttggaatct gcttttctgc ctgctttctg
     2341 ttataataga agacatctta tctgaaaaca cactagaaag gtccatatga cgaaaaaaat
     2401 tattgcagtt gacctagatg gaaccctgct caactcagac agtcaaattt ctgactttac
     2461 caaaaaaacc atcaaaaaag ttgctgaaaa aggccatcag gttattatta cgacaggtcg
     2521 cccttaccgt atgtcaaaag atttttaccg tgaactgggc ttagacactc ctatgattaa
     2581 cttcaacgga tcccttactc atttaccaga ccaagtttgg gattttgaaa agtgtttgac
     2641 tgtagacaaa aaatatctgc tagatatggt tcaacgttca gaggacattc aagccgattt
     2701 tatcgctgga gaatatcgta aaaaattcta cattacaaat cccaatgaag aaattgccaa
     2761 tcccaaacta tttggtgtag aagctttcca gcctgaagat caattccagc ctgaattggt
     2821 gaccaaggac cctaactgta tcctcttgca gaccagagcc agtgacaaat atgccttggc
     2881 aaaagaaatg aacgccttct accagcatca actttctatc aatacctggg gaggtccgct
     2941 caatatcctt gaatgtaccc caaaaggtgt caacaaggcc tttgctttgg actacttgct
     3001 caagataatg aatcgtgaca aaaaagattt gattgccttt ggagatgaac acaatgatac
     3061 cgaaatgctc gcttttgctg ggaagggtta tgccatgaaa aatgccaatc cagagctact
     3121 cccttatgca gatgagcaaa tttcccttac caacgaccaa gatggggttg ccaaaaccct
     3181 acaagactta ttcttataac ctatactgat actcaatga
//
LOCUS       DAVBCW010000143         3198 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVBCW010000143 DAVBCW010000000
VERSION     DAVBCW010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.143"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            <1..128
                     /locus_tag="VQ514_001938"
     CDS             <1..128
                     /locus_tag="VQ514_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2500660.1"
                     /translation="ENIFAKVKTFKIFSTTYRNHRKRFGLRMNLSAGIINHELGF"
     gene            complement(125..370)
                     /locus_tag="VQ514_001939"
     CDS             complement(125..370)
                     /locus_tag="VQ514_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HET2500661.1"
                     /translation="MEQSPQKKKGTTLEHIILLRGVTPNGKNAIPKMSYLVDILTEAD
                     FQQVRTYIQSGNIILESNLALEEIREQVHTLLDFLQN"
     gene            complement(385..1557)
                     /locus_tag="VQ514_001940"
     CDS             complement(385..1557)
                     /locus_tag="VQ514_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET2500662.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSNPI"
     gene            complement(1616..2461)
                     /locus_tag="VQ514_001941"
     CDS             complement(1616..2461)
                     /locus_tag="VQ514_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET2500663.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEDGGLLM
                     GYEID"
     gene            2586..3143
                     /locus_tag="VQ514_001942"
     CDS             2586..3143
                     /locus_tag="VQ514_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET2500664.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
BASE COUNT          959 a          639 c          647 g          953 t
ORIGIN      
        1 ttgagaacat ctttgccaaa gtaaaaacgt ttaaaatatt ttcaacaacc tatcgaaatc
       61 atcgtaaacg cttcggatta cgaatgaatt tgagtgctgg tattatcaat catgaactag
      121 gattctagtt ttgcaggaag tctaatagag tatgaacctg ttctcgtatt tcttcgagag
      181 ctaagttact ttcaagaata atatttccac tttgaatata agttcgaact tgttgaaaat
      241 cagcttctgt caagatatct actagataag acattttagg gatagcattt tttccattag
      301 gagtgactcc ccttagcaag ataatatgtt ccaaagtagt tcctttcttt ttttgggggg
      361 attgttccat ttagtaatgt atatttagat tggatttgat cccgccaccc agccggtaca
      421 gagggcagaa gtgatgttaa agccacctgt gtgggcattg atatccataa cttcgcctgc
      481 aaagtggagg ccaggtacca gcttactttc aagggtttta ggattgattt ccttgagact
      541 gactccaccc ttggtaacaa aggactttgc aagggacatt tttccagtta caggaatttt
      601 aagttcttta atggactgga caagttgttc tcgttccttt tcagtcagtt gtttgacttt
      661 ttcaggatat ccttgtacaa aaaattcggc caagcgttct ggtaacaagg tttttaaagc
      721 gtttttcaag gatttttccc gattttcttc tagaaatgta accaagtcct tctcagaaag
      781 ttgaggcaaa acatcgagtg agagaacctc cccacctttg acaaagctag acatgcgtag
      841 ggcagcagga cctgacaaac caaagtgggt aaagagtaaa tcatgagtga tgacatgctt
      901 accataactt agggtcacat cgtccaaaga aatcccttgt aaggctttat gtggaaaatc
      961 tgttaataaa ggactttcag cagcctcaag atcggtgatg gtatgcttaa aatggcgagc
     1021 aatctcgtga ccaaaaccag tcgaaccagt cgaaggataa gacttaccac ctgttgtgac
     1081 aatgagtttc tcacaagtga aggtttgatc cgctgactta aggacaaact ggtcatctac
     1141 ttttttaaca gaaacgattt ctatttgagt agcaacttga ccacctagtt cggtgatttt
     1201 cttttccaaa gcttcgataa tagtccgaga cttgtcactg gctggaaaga cgcgtccgtg
     1261 gtcttcgacc ttaagtttaa caccattttc tgtaaaaaag ttgatgatgt catgattatc
     1321 gaactgggag aaaacactgt aaagaaagcg tccgtttcca ggaattccag ctagcaggtt
     1381 gtctaagcta ccattgttgg tcacattgca acgtccccca ccagtcccag ctaatttttt
     1441 tccaagtttc cgattttttt cgatgaggag ggttttctgt ccataaaagc tactggaaat
     1501 cgtagccatc ataccagcag gtcccccacc gatgacaata gtatcaaaat gtttcatagc
     1561 tctattgtac cacaaaaaaa caagagatga tggtcacctc ttgtcaagaa tacaattaat
     1621 caatttcata gcccatcagc aaaccgccat cttctgcata gaaactgcag agaccagagg
     1681 ttggtagaat tttaatatcc gcttgtggga aggtttcacg gattcgctct gagagctgtt
     1741 gacaacattt ttcgttattg cgttgggcca tgacaatacg gccaccagca tatccagctt
     1801 ttactaactc atcataggca gcttgaactg atttctttga tccccttgct ttttgtagca
     1861 attcgagagt tccagtttca ctagcttttc cgaccatacg aatgttgaga aggccaacga
     1921 ccgtaccgat aagcttgctc aaacggccgt tcttcaccaa gttatcgact ttggctagga
     1981 caaagagcaa cttagttttt tcttgatagg cggtgatagc ttcaaccact tcttcaaaag
     2041 acaagccctg gtcaatcaag tcattcaatt tttctacgag taggtcaact tcaccaccag
     2101 cagataaact atcaatcaca tgaatcttag tgtcaggatg gtcttccaga taaatattct
     2161 ttgctagttg agcactattg tgactgccag aaagggtacc tgtgatggtt actaggaaaa
     2221 tgtttttggc accttcaaat gctcgcaaat agtcatctgg gcttggacaa gccgattttg
     2281 aagcttctgc agttgcatac atggtttcca tcatttggtc aatatcgaga ctggcgtcat
     2341 caacaaagac ctgatcagct acttgaatgg ttaaggggac acttacaaag gttgtgttaa
     2401 tagctggtgt tggcagttga cgataatcac aaccagagtc agcaataatc ttccaagtca
     2461 tagaaattct ccatctttgt cagttataca ttgacaaagg ttctgtcttt ttttacaatt
     2521 atatcatgaa aacccttgaa acaaaagcat catccgtctg ttgtgacaag tagaaagaaa
     2581 atgttatgtc tgaacgtaga atctctgaaa agtctcttga aaatctcaga aaatcaaacc
     2641 aagaatccaa tttattaacc agagaagcca ttgaaacagc cctcttgcaa ctcttggaaa
     2701 aaaaggaact gaccaagatt agtatttctg aattggtcaa acgtgcaggt gtttcgcgtg
     2761 cggcctttta tcgcaattat gactccaaag aggaaatttt agagagcgtc tttaaacgaa
     2821 ctgtccacaa tattatggaa cagctgcatc attacgattt aaagacagac ctttatttgg
     2881 tttgggttca ccttttccgg gaggccagaa aggaagctag agtaattcaa ttggccttgg
     2941 attaccatct ggaaaaaatc tttgtccaag ccatgcagga atttctagaa aaataccatg
     3001 ggaaatcaaa aggtgtcagc tcttatcttc attccttctg gagctcagcc atcgtctctg
     3061 tccttctaaa atggatcaag gatggcatga aggtaccagc tgaaaagatt gcagatttag
     3121 gtttaccatt ttttaaaaaa tagagaaaaa ggagaaaaga gatgactgaa aaaagactgg
     3181 cctgggatga gtattttg
//
LOCUS       DAVBCW010000144         3073 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.144, whole genome shotgun sequence.
ACCESSION   DAVBCW010000144 DAVBCW010000000
VERSION     DAVBCW010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.144"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            49..764
                     /locus_tag="VQ514_001943"
                     /pseudo
     CDS             49..764
                     /locus_tag="VQ514_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(730..2205)
                     /locus_tag="VQ514_001944"
     CDS             complement(730..2205)
                     /locus_tag="VQ514_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET2500665.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNPEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEEVIELSEYARHEINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDDSVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
     gene            2912..>3073
                     /locus_tag="VQ514_001945"
     CDS             2912..>3073
                     /locus_tag="VQ514_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HET2500666.1"
                     /translation="MQDNYTTKGKHLTIDSRRLIERWKKEGKSNREIASLLGKAPQTI
                     HTKIKRRTVR"
BASE COUNT          960 a          671 c          544 g          898 t
ORIGIN      
        1 tcgtggataa ccaagtagac ttgaaatact acatctggag gtactcccat gctttatgtg
       61 ggcattgatg tcgctaaaaa taaacacgat gttacagcct tgaatgttca aggaaaaact
      121 gttcttaaac cactcacttt ttcaaataat aaagctggtt ttgaactctt agatctgtct
      181 cttcgacagc tcaaccaaga ctgtctcatc gctcttgaag atactggaca ttatgccttt
      241 aatctactga acttcctgca tgaacaagga tataaagttt acacctacaa tcctttgctc
      301 atcaagaaat ttgccaaatc attatcactt cgcaagacta aaacggataa gaaagacgct
      361 catggcatcg ctcttaaact tctttctgac cccaatcgtg aacaatttca acacaataat
      421 cggcaagtag aactaaaaat actgactaga catattcatc gtctcaagaa aaaacagtct
      481 gattggaaag tacaatacac tcgttgcctt gatatcatct ttcctgagtt ggataaaatc
      541 gttggaaagc attcagaata tacctaccaa ctcttgacac gctaccctaa tcctcagaaa
      601 aggattgagg caggatttga taagctgata gaaattaagc gattgaccgc ttctaattca
      661 agggcttttt gccgtgctct aaaatcatcg ccaaatcatc aaattgaaat aagaaatagt
      721 ttagataaac tattgacttt tcttatagtt tgtctttctc ttaataacgt tgatatgatt
      781 gacctctgga tcttccgtcc cttggaggga gcaaccacgt tccttggcga attggatata
      841 gtcgacaatt tctcgtgtaa tgcgttcacc aggagccaag ataggaatac ctggagggta
      901 acacataaca aattctccac agacctgtcc aacagaatca tccaaagtta aactttttct
      961 ttctgaatag aaggcttctt gcggagacag cactaactcg ggctgaatat attctcctgc
     1021 tatcaaatct tttccatctc ttgaatagag tctcttaata tcagccagag caccaaccaa
     1081 gcgctcgatg tcttggatgc ggtcgccgat ggaaatatag gccaagatat tgccgatatc
     1141 accaaactcg atctgaatgt cgtattcgtc tcgcaagagg tcataaacct cgatacctgt
     1201 taagccaata ccctgagtgt aaactgacaa cttagttacg tcaaaatcgc aaaccgaaac
     1261 accgtctatt aactcttttg agtaggcata gtagccaccg atagcattga tttcatgacg
     1321 agcgtactcg gatagctcaa tgacttcctc aaacgactct ttaccacgaa gggccaagtt
     1381 gcgacgtgaa atatccaaac tagccatcaa taagtaagag gcagatgtag actgggtcag
     1441 gtttataatc tgacgaacgt actcgggatt catctgctcc ccgattaaaa gaagcgaact
     1501 ttgtgtcaaa ctcccaccag acttatgcat ggaaactgct gccatatcag ctcctgcatc
     1561 catagcagaa attggaagtt taccagtgaa atgcaaatgc gctccatggg cttcatctac
     1621 taaaaccatc atgccagctt catgagccat ttctgtcaac ccctttaggt ctgaacagat
     1681 gccgtagtaa gtaggattgt tgattaggat agccttagca tctggatggt cctttatggc
     1741 ctgtgctact cggtcatttt caagacctaa agcgatacca atcttaggat ctacactcat
     1801 ctcgatatag atgggaatgg caccacatag aaccaacgca ttgatagcag atttatggac
     1861 atttcgtggc agaataatct tatctcctgc cttgcaggtt gccagaatca tagtctgcac
     1921 cgatgaagtt gttccaccaa tcattagaaa ggcatggcta gctccaaaag catctgcagc
     1981 cagctcctct gcatcacgaa taatcgaaat aggatggcct aaattatcca agggtttcat
     2041 cgaattgaca tcaatgccta cacatttttc tcctaagagt tcgacaagtt ctggatttcc
     2101 ccgtccacgc ttgtgacctg gaacatcaaa gggaacaatc cttttcttgc gtaacttcac
     2161 caaggcctca taaattgggg cttggttttg atctaactct ttcaaaacat actcctttca
     2221 atatttttag gaccttatga aaactaagga actaaaggtt gactcatgaa aaaagagcag
     2281 gttttcacct gctctgcaat cttctgacgt gtttattttg gtttctgttg agtatgtctg
     2341 ttcccgatgt ttcctaactc tctagtagca aatattacgt actttattga tcgtttcaca
     2401 agatgacgtg tgctttcacc acactaaaaa tttatgaatt aggacaagtg tcctaacatc
     2461 aacttataaa agtaggcttt taaccctatc aataatgtgg cttttcaacc acgcttcggc
     2521 attcaaccct gcctgtccgt aggcattttt tactcgggtt tatatttgct agaaaacatc
     2581 atctcatttt ctaagcttta ttactatatc atgtttttaa gaacttgtaa agtatttttt
     2641 gataaaaaac aaaaatataa tagattgaaa ctagaatagt acacctctgc ttctaaaaca
     2701 ttgttagaaa tagattttac tgtcctgatc gatttgttct gttcttgttt cattttacta
     2761 tatgttcgta aatgaaagtt ctataatgaa gttagccacc cagccctcct aaaaattaga
     2821 tggttcaact agctgatagc tcttgtaggc ataagaaaac acctctgtgt tatacttgtt
     2881 gttcaaccac aaacacaaga aaggcacaga aatgcaagac aattatacta caaaaggtaa
     2941 acatttgaca atcgatagcc gtcgcttaat cgaaagatgg aaaaaagaag gaaaatcaaa
     3001 tagagaaatt gcctctctac ttggaaaagc tcctcaaact atccacacta aaatcaagcg
     3061 taggacagtc cga
//
LOCUS       DAVBCW010000145         2953 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.145, whole genome shotgun sequence.
ACCESSION   DAVBCW010000145 DAVBCW010000000
VERSION     DAVBCW010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.145"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            153..437
                     /gene="groES"
                     /locus_tag="VQ514_001946"
     CDS             153..437
                     /gene="groES"
                     /locus_tag="VQ514_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HET2500667.1"
                     /translation="MLKPLGDRVVLKIEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            453..2075
                     /gene="groL"
                     /locus_tag="VQ514_001947"
     CDS             453..2075
                     /gene="groL"
                     /locus_tag="VQ514_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HET2500668.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            2213..2953
                     /locus_tag="VQ514_001948"
                     /pseudo
     CDS             2213..2953
                     /locus_tag="VQ514_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567576.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
BASE COUNT          932 a          570 c          680 g          771 t
ORIGIN      
        1 ttaaaaagtc tgagttggtc tcaggctttt tatcttgaga aagtcagact tttttcttga
       61 ttatttctga ctaagtgata caatagaact atgaattagc actcaggtat aaagagcgct
      121 aataatatct atctcattat ggaggaaatc agatgttgaa accattaggg gaccgtgtgg
      181 tcttaaaaat agaagaaaaa gaacaaaccg ttgggggctt tgttctcgca ggttcagccc
      241 aagaaaaaac caaaacagcc caagttgtgg ctactggaca aggtgttcgt accttgaacg
      301 gtgacttggt tgctccaagt gttaaaactg gagaccgtgt cttagttgaa gcccacgcag
      361 gtcttgatgt caaagatggc gatgaaaagt acatcatcgt aggcgaagct aacatcttgg
      421 caatcattga agaatagaag gagaaagtaa gtatgtcaaa agaaattaaa ttttcatcag
      481 atgcccgttc agctatggtc cgtggtgtcg atatccttgc agacactgtt aaagtaacct
      541 tgggaccaaa aggtcgcaat gtcgttcttg aaaaatcatt tggctcacca ttgattacca
      601 atgacggtgt gactattgcc aaagaaattg aattagaaga ccattttgaa aatatgggtg
      661 ccaaattggt atcagaagtg gcttcaaaaa ccaatgatat cgcaggtgat ggaactacaa
      721 ctgcaactgt tttgacccaa gcaatcgtcc gtgaaggaat caaaaacgtc acagcaggtg
      781 caaatccaat cggtattcgt cgtgggattg aaacagcagt tgccgcagca gttgaagctt
      841 tgaaaaacaa cgccatccct gttgccaata aagaagctat cgctcaagtt gcagccgtat
      901 cttctcgttc tgaaaaagtt ggtgagtaca tctctgaagc aatggaaaaa gttggcaaag
      961 acggtgtcat caccatcgaa gagtcacgtg gtatggaaac agagcttgaa gtcgtagaag
     1021 gaatgcagtt tgaccgtggt tacctttcac agtacatggt gactgatagc gaaaaaatgg
     1081 tggctgacct tgaaaatccg tacattttga ttacagacaa gaaaatttcc aatatccaag
     1141 aaatcttgcc acttttggaa agcattctcc aaagcaatcg tccactcttg attattgcgg
     1201 atgatgtgga tggcgaggct cttccaactc ttgttttgaa caagattcgt ggaaccttca
     1261 acgtagtagc agtcaaggca cctggttttg gtgaccgtcg caaagccatg cttgaagata
     1321 tcgccatctt aacaggcgga acagttatca cagaagacct tggtcttgag ttgaaagatg
     1381 cgacaattga agctcttggt caagcagcga gagtgaccgt ggacaaagat agcacggtta
     1441 ttgtagaagg tgcaggaaat cctgaagcga tttctcaccg tgttgcggtt atcaagtctc
     1501 aaatcgaaac tacaacttct gaatttgacc gtgaaaaatt gcaagaacgc ttggccaaat
     1561 tgtcaggtgg tgtagcggtt attaaggtcg gagccgcaac tgaaactgag ttgaaagaaa
     1621 tgaaactccg cattgaagat gccctcaacg ctactcgtgc agctgttgaa gaaggtattg
     1681 ttgcaggtgg tggaacagct cttgccaatg tgattccagc tgttgctacc ttggaattga
     1741 caggagatga agcaacagga cgtaatattg ttctccgtgc tttggaagaa cccgttcgtc
     1801 aaattgctca caatgcagga tttgaaggat ctatcgttat cgatcgtttg aaaaatgctg
     1861 agcttggtat aggattcaac gcagcaactg gcgagtgggt taacatgatt gatcaaggta
     1921 tcattgatcc agttaaagtg agtcgttcag ccctacaaaa tgcagcatct gtagccagct
     1981 tgattttgac aacagaagca gtcgtagcca ataaaccaga accagtagcc ccagctccag
     2041 caatggatcc aagtatgatg ggcgggatga tgtaagcttt ctatagaaaa caacttataa
     2101 aaaacacaaa aggagggaat gactaaccct tctttttata atattcactt ctaaattgat
     2161 ttgagctctt ctaacttata tgataaaata agactagaaa aaggagaaga acatgatcga
     2221 tgtagaagaa attctgtgca agatgccccc caatcagaag attaattatg accgtgtcat
     2281 gcagaaaatg gtacaagcat gggagaaaaa tgagcagcgg ccaaccattc tcgtgcatgt
     2341 ttgctgtgcc ccttgtagta cctatacact agaatatttg accaagtatg cagatgtgac
     2401 catctatttt gccaattcta atatccatcc caaagtagaa taccataagc gggtctatgt
     2461 catcaagaaa tttgttagtg attttaatga tcggacagga aatacggttc agtacctaga
     2521 agctccctac gaacccaatt aataccgaaa actagttagg ggactagagg aggagcccga
     2581 aggtggcgac cgttgcaagg tttgttttga ctaccgactg gataaaacag cgcaagtggc
     2641 tatggacttg ggctttgact actttggttc agccttgacc atcagtcctc ataagaattc
     2701 tcaaaccatc aatagcatcg gaatcgatgt gcaaaaaatt tacacgaccc actatcttcc
     2761 caacgatttc aagaaaaatc aaggctacaa acgttcagta gagatgtgtg aggagtatga
     2821 tatctatcgt caatgttatt gtggctgcgt ctatggcagc ccaagcccag aatattgacc
     2881 tggttcaagt taagaaggac gccacagcat gcttgttgga taaggatgtt gagaaagact
     2941 attctcatat caa
//
LOCUS       DAVBCW010000146         2891 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.146, whole genome shotgun sequence.
ACCESSION   DAVBCW010000146 DAVBCW010000000
VERSION     DAVBCW010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.146"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(91..1140)
                     /locus_tag="VQ514_001949"
     CDS             complement(91..1140)
                     /locus_tag="VQ514_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2500669.1"
                     /translation="MKDLFLKRKQAFRKECLCYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEAIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LDWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(1137..1868)
                     /locus_tag="VQ514_001950"
     CDS             complement(1137..1868)
                     /locus_tag="VQ514_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2500670.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            1936..2346
                     /locus_tag="VQ514_001951"
     CDS             1936..2346
                     /locus_tag="VQ514_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HET2500671.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMNATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            2356..2643
                     /locus_tag="VQ514_001952"
     CDS             2356..2643
                     /locus_tag="VQ514_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500672.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIIDQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
BASE COUNT          967 a          649 c          549 g          726 t
ORIGIN      
        1 tctctacgga ccaaccatta tcctgtagta acaaaaaaga agctgagaca gactgaactc
       61 aacttctttt ttagtgtcgt atcagcaatg ttagtcctgc atctgacgtt ttacctgata
      121 aggcaaatac aagactagta aaaccaaacc agctcctagt aaggctagaa gggccagttt
      181 ttcttggaag gtaatcaacc caacaactaa ttccacaagt aaaacaaaac tggtcaatcc
      241 tcgaactacc tcctgtaagc ctttttcctt ttgccccttg tccagcggaa agagttgggt
      301 caaatactgg tagtcaaagg catgatagag ggccagcaac tggaagagca agaggtagtt
      361 aaagagaact accactgctg tcgcaatcca agcttgctcg ataaaaacct gcgccagtac
      421 ggaaagtaat agaagacgaa gactgagagc aaagaggtcg ccatttcgca gataagaacg
      481 cagatagaga ttttgccaaa tcttcccagg caccttctga acagccttta agataaagtc
      541 cagataggca cgacgcttaa cgctgtttga aattcccttg acctgcgtaa agagggcaaa
      601 gaaacgaagc aagacttgct tacgcttgct ttcttgagaa ataacatagt cccagtccag
      661 tccagtttca gtaaaaaatt tgctggcctt ttgacgaaag tggaaatatt ttcctacccc
      721 caataaaagc acatatagca gaaaaactgg caagccataa cccattgcta aaaataaagg
      781 cgcaaataac agcaagaaaa gggtctgtac aaagagccaa aagactaggg aaatgccagt
      841 ttgacgcttg agatggagct tgattgcctc ttctccaact aagagaaaga gcttgtctgg
      901 agcctccata taggtggcaa ttcctcccca aagtaagagt aaaacagacg taattcctac
      961 aaacaaaagg ataggccaat gattttcagg aaaatgttgt aagagttgac tgtactggta
     1021 ggctagaaag cccaacagga caagcaggaa caagacaaag tggtcattga gcacatagcg
     1081 cagataacaa agacactcct tacgaaaggc ctgctttctc tttaaaaaca agtctttcat
     1141 agatcctcct ctttggtcag agccaagtaa atatcattca aactagcctc aggcatatca
     1201 aaggcttcac gtagttgcag gagattgcct ttggcacgca cctctccctt gtgaagaatg
     1261 acaaaggcat cacacatctt ttccgccgaa tccagcacgt gggtactcat gagaatagac
     1321 ttgccctttt gcttctccac ttccaaaagc tgaatcaaat cagaaatagc cagcggatca
     1381 agaccaagga aaggctcatc cacgatgaaa agacttggat ccaccacaaa agcacagata
     1441 atcatgacct tctgcttcat cccttttgaa aaatgaacag ggaaccagtc taatttctgt
     1501 tccaaacgga acatttttaa caagggctct actcgttcga aagccacttt ttgctcaata
     1561 ccgtaagcca tagcaaccgt ttcgatatgc tctctgaggg tcaattcctc atacagacta
     1621 ggcgtctcag gaatgtagcc aatctgcttg cggtagctag tcgcatctcc ttgcagagtc
     1681 aggccattga tattgatgga gccactataa ggtgccaaca gaccgataat ctcattgatc
     1741 gtcgttgatt tcccagcacc attgagacca atcaaaccga ccaactgccc actttcaaca
     1801 gtaaaggaca catctttcaa aacaggaaca tgaacatagc cacctgtcag gtttttaatt
     1861 tctaacatat tttctccaaa tctggtataa tgtagctata ttatatcaaa attcagtaca
     1921 gtagaggtag attttatgtc agattgcatt ttttgtaaaa tcatcgcagg ggaaattcct
     1981 gcttcaaaag tatatgaaga tgagcaggtc cttgcctttc ttgatatctc tcaagtaaca
     2041 ctaggacaca ccttggtcgt gccaaaagaa cactatcgca atcttttgga gatgaatgct
     2101 acgagcgcca gccaactctt tgcccaagta ccaaaagtag ctcaaaaagt catgaaagtc
     2161 actaaggctg ctggtatgaa tatcatttcc aactgtgaag aagtcgctgg tcaaacagtt
     2221 tttcatactc acgttcacct tgtgcctcgc tacagtgctg acgatgacct caagattgat
     2281 tttatcgccc acgaaccaga ctttgacaaa cttgctcaag tcgctgaaac catcaaaaac
     2341 gcttaaggag ttgctatgaa attatccaac ctactgctat ttgcaggagc tgcagccgga
     2401 agttatctgg ttacaaaaaa tcgccaaacc atcacagatg aagtcttgaa taccactgac
     2461 cgcgttcaag ctatcaaaga cgatgtggat attatccaaa atagcctgca aatcatcgac
     2521 cagcaaaaag aacttatcaa ggaataccaa gaagacttga cttacaagtt taaggtcttg
     2581 gaaaaggata tccagactag actagctgtg ataaaagaaa tgcagggaac tgaagataag
     2641 taaaaagagc cccccggctc ttttttaaaa cgtaaacaag ttatagtgaa ttgaatttag
     2701 aatagcacat catagtttct aaagcattgt tagagattac tttaaaatcc tcttatcaat
     2761 ttgttcatat tctatttcaa tctactatat atatcccatc aactatgcat cataatttag
     2821 gtaactcata ctcaataaaa atcaaaaagc aaactagaaa gctaggcaca gactgctcaa
     2881 aacaccgttt t
//
LOCUS       DAVBCW010000147         2856 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.147, whole genome shotgun sequence.
ACCESSION   DAVBCW010000147 DAVBCW010000000
VERSION     DAVBCW010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.147"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(<1..941)
                     /locus_tag="VQ514_001953"
     CDS             complement(<1..941)
                     /locus_tag="VQ514_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HET2500673.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(919..2682)
                     /gene="aspS"
                     /locus_tag="VQ514_001954"
     CDS             complement(919..2682)
                     /gene="aspS"
                     /locus_tag="VQ514_001954"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HET2500674.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGTVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEQEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAIVVKEAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVFFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETVHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSTEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
BASE COUNT          828 a          676 c          569 g          783 t
ORIGIN      
        1 taattatcta tctcaacaaa tcttcctaga atatgaacat tttccgagac agagacaaag
       61 gagcttggat ccacttgtgt cataatctgt ttaaattcat taaactctgc acgtgtaatg
      121 acagtgatta aaactgcctt tctctcgtga ttataggttc cttctgcatc gtggatcatg
      181 gttgctccgc ggtgcaattt tttatggatt ttttcaatta ccttctctgg atgatttgtc
      241 acaatcatgg cctgcatacg cttttgctta gtaaagactg cgtctgtcac acggctagag
      301 acaaagatgg taatcataga ataaagagcg tatttccaac caaaggtcaa acctgctatc
      361 agcatgatag ttccatttac caagaaagaa atactaccga cattcttacc cgttttctta
      421 cgaatagtca ggctgacgat atccgtccca ccactggaga tattgtttcg aagagcaaaa
      481 ccaatcccca aacccataac aacaccccca aaaagggaat tgataatggg atcctccgtc
      541 aaggttgcca cagggacaaa ctggataaag aaggaactca tagataccgt gataaaggta
      601 aagacggtga acttatggcc aatctgatac caagctaaga ccatcaaagg gaagttaatg
      661 gcgtagaagc ttagcgaaat cggaatatga aaaccaaacc agtgattact caaggcagag
      721 ataatctgtg ccagacctgt tgcaccactc gaatacacat gccctggttg gaaaaagaaa
      781 ttaactgcta ctgctgataa aaaaccatag accagagagg ccgaaatctt ctcatcatac
      841 ttttctcgag agatactttg taagacacgt aaaattttta tctgataagc aaagcggcgc
      901 agataatagc gccaccgctt aattcgtttt gtttgtttca tcttcttcta cttgtaagct
      961 gagttcctct agttgtttga gagcgactgt tgatggagct tgtgtcattg ggtcagttgc
     1021 cttgttgttc ttaggaaagg caatgacttc acggatattt tcttctccag caagcaacat
     1081 gacaaaacgg tcaagcccga tagccaaacc accgtgtggt gggaaaccat agtccatggc
     1141 ttcaagaagg aaaccaaact ggtcattggc ttcttcagtt gagaaaccaa gagccttgaa
     1201 catgcgttct tgaaggtctt tttggttgat acgaaggcta ccaccaccaa gctcataacc
     1261 gttcaagacg atatcgtaag caatggcacg aaccttagcc aaatcacctt ctaattcgtg
     1321 aacagtctct tcctgtggaa gggtgaaagg atggtgggcg ctcatgtagc ggccttcttc
     1381 ttcagaccat tcaaacatcg gccagtcaac cacccaaagg aagttgaact tatcattatc
     1441 aatcaagcca agctctttag caatacgtcc acgaagggca cccagtgttg cattagccac
     1501 ttcaagcgta tccgccacaa agaaaaccaa gtccttatct tcaagagcaa gcgctgttgt
     1561 caattcttct tggataccag tcaagaactt ggcaactggt ccgtttaatt ctccatcaac
     1621 caccttgacc caagcaagac ctttggcacc atactgtttg gctacttccg tcatcttgtc
     1681 gatgtcttta cgtgaatagt tgtccgcagc ttctttgacc acaatcgctt ttacagcagg
     1741 tgcttctgaa aagactttaa agtctacacc tttgaccact tctgtcaagt cctgaagcaa
     1801 catgtcaaaa cgagtatctg gcttgtcaga accgtaaaga gccatagcat catcgtattt
     1861 catacgaggg aatggtagcg ttacttcgat gccttttgtt tccttcatca cgcgcgcgat
     1921 caagccttct gtaatatctt ggatttcttg ctcagtaagg aaggacgttt ccaagtcgac
     1981 ctgagtaaat tcaggctggc ggtctccacg caagtcctcg tcacggaaac atttaacgat
     2041 ttggtagtaa cggtcaaaac cagcattcat caagagctgt ttcgtgattt gtggactttg
     2101 aggaagagcg taaaaatgcc ccttattaac acgagacggc actaaataat cacgcgcccc
     2161 ttcaggcgtt gacttagaaa ggaatggtgt ctccacgtcg ataaactcca actcatccaa
     2221 gtagttgcgg atagagtggg tcaccttggc acgaagttta agattttcca acatttctgg
     2281 acgacgaagg tcaaggtaac ggtaacgcaa acgtgtatcg tcatttgcct caatgccatc
     2341 cttaatctca aatggtgttg tcttagctgt gttaagcaca ataagagctg tcacgtttaa
     2401 ctcaaccgta ccagttggca acttatcatt ggcttgctca cgcgcagcga cctgaccagt
     2461 cacctcaata acaaattcgc tacgaaggct ttcagctgtt gccataacct ctgcagatac
     2521 tttttcaggg ttgataacca actgcatgat tccttcacgg tcacgaagat cgataaagat
     2581 caaaccacca aggtcacgac gacggccaac ccatcctttc aaggttattt cttgtccgat
     2641 gtgttcctca cgaacacgac cagcatacat actacgtttc attatttctc tcctctttta
     2701 ttctgttact attttaccat aaaagcgcag gctcttcatg aaaatcatca gaaaagtttg
     2761 ccagtcttta aaagtcaggt gaaagcccta aaaattagcg ctaatactct tcgaaaatct
     2821 cttcaaacca cgtcagcgtc gccttgccgt aggtat
//
LOCUS       DAVBCW010000148         2840 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.148, whole genome shotgun sequence.
ACCESSION   DAVBCW010000148 DAVBCW010000000
VERSION     DAVBCW010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.148"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            87..2783
                     /locus_tag="VQ514_001955"
                     /pseudo
     CDS             87..2783
                     /locus_tag="VQ514_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
BASE COUNT          876 a          458 c          624 g          882 t
ORIGIN      
        1 gcttgtgaga tagctataaa agtgtggaga aatagcttga aatttagtat aatatagcta
       61 aactatttgt ttaaagtgag aaaaaaatgg gaaattttag ctttctttta aaaaatgacg
      121 aatatgaatc tttttcaaaa ccttgcattg aagctgagaa tatgattgct acatcaactg
      181 tggctactgc ctttatggcg cgtcgtgctt tagagcaggc tgtccattgg atatatagtc
      241 acgattcata tttagaagct ccctatcgtg ctactctatc ttctttagta tgggatgatg
      301 attttaggga tatcgtagat tctgaactcc acaagcagat agttctgttg attcggtggg
      361 gaaaccatgc tgctcatggt ggtgaaatta aggaacgaga agcgatttta gctttgcatc
      421 atttgtatca gtttgttaat tttatcgatt attgttacag caatgagttt gtggagcgtt
      481 attttgatga gaagtgctta ccactttcag caaacatcaa ataccgagaa actccacaat
      541 ctatgataaa gttacaagac agtttaccag aactgcctga ttttcatgaa cagatggctg
      601 ctcagtccgt agaagttcaa gagacttata ctgaaaaacg tgagactgca gcgcaacggc
      661 aagatgtgcc tttccatatt gatcaattat ctgaggcaga gacaagaaag ctctttattg
      721 atatcgatct ccgtttagca ggatggatat ttgaagaaaa ctgtcgtgtt gagatagccg
      781 ttgatggtct caagcacggt tcaggaattg gttactgtga ctatgtactt tatggtaaaa
      841 atgggaaaat tttagcgatt gtggaggcta aaaaagcctc tgtcaatcca gaagtagggg
      901 aagtacaggt caaagaatat gctgaagctt tggagaaaca tatcggctat cagccaattt
      961 gctttattac aaatgggttg aagcactata tacttgatgg tccgaaccgc cgccagattg
     1021 caggctttta ctctcaagaa gaattgcaat tagtgatgga tagacgtcat cttcaaaaac
     1081 cgcttgagga tatttctagt aaaattaggg acgatatttc cgggcgtcac taccaaaaac
     1141 atgccattgc aagcgtttgt gaagctttct ctgatcatcg tagacaggca cttttggtta
     1201 tggcaactgg ggcggggaaa actcgtacag cagtttctct agttgatatc ttatcacgtc
     1261 ataactgggt aaaaaacgtt ctcttcttag ccgatagaac ttccttggtt aagcaagcat
     1321 atgattcgtt tagaaaatta ctcccagatc tttccgtttg taacttctta gaagataaag
     1381 aaggagctca atcaagtcga atggtctttt caacttatcc gaccatgatt ggagcgatta
     1441 gtggtcaaga agaagtaaat caacgccctt ttactgttgg gcattttgac cttatcataa
     1501 ttgacgaatc tcaccgttct atttatcaga aatacaagtc catttttgat tattttgatg
     1561 caaggattgt aggcttaaca gctactccgc gtcaagattt agataaaaac acctatggat
     1621 tctttaattt ggagaatggg gttccaacat atgcatatga tttggaagag gctgttaaag
     1681 acggatattt agtagcctat cattctatcg aaaccaaact gaaactacct acggatggtc
     1741 tacattatga tgatttgtcc gaagaagaaa aggaacattt tgatagcaaa tttgaagacg
     1801 atagctgtga aaaagatatt gatgggagtg tgtttaattc ctttattttc aataaaagta
     1861 cagtagaaat tgttttaaat gaactcatga caagaggaat tcagacagcc tcgggtgatg
     1921 aaattggtaa aactattatt tttgctaaaa atcatgacca tgcggaatat atcagaggta
     1981 tttttaacaa ccgctatcct gaaaaaggga gcgactatgc tcaggtgatt gattatagta
     2041 ttaagcatta tcagaccttg attgatgatt ttaaaattaa ggagaagtat cctcaaattg
     2101 cgatttctgt cgatatgtta gatacaggta ttgatgtacc agaggttgtt aatttagtct
     2161 tcttcaagaa agtacgctct aaaactaagt tttggcagat gattggtcga ggaacccgtc
     2221 tatgtaaaga tttatttgga cctgagcagg ataaggaaaa cttcttggta tttgattatg
     2281 gggacaattt tgattatttt cgtgcagatc caagagatgg agagggtcgt cacattgttt
     2341 cgctgactca gcgtttattt aatatcaaag tggacttgat tcgagaactt cagggactcc
     2401 aataccaaga agatcagttt gcgagagcat accgtcagca gcttgtctcg gaacttcaag
     2461 gtcgtataga gagcttaaat gagttggact tcagggttcg tatggtttta gatacagttt
     2521 atagctatag gaaattggaa agttggcaga atctaactgc tgttacaagt gaaaccattc
     2581 aaaaaaatct ctctccgctt ttatttgatg aagataaaga agatgagatg gcgaggagat
     2641 ttgatttgtg gttgcttcat attcagttgg ggcaactgac agctaaatct tccactgttc
     2701 atatttccca agtgatgaag acggctagag ctctttctgc tattggcaat atcccgcagg
     2761 tttttgagca ggctgaaatt atggctcttt gtcaactgta gtgggttgaa aaaaagctaa
     2821 gcttgagaaa ggacaaattt
//
LOCUS       DAVBCW010000149         2828 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.149, whole genome shotgun sequence.
ACCESSION   DAVBCW010000149 DAVBCW010000000
VERSION     DAVBCW010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.149"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(381..914)
                     /locus_tag="VQ514_001956"
     CDS             complement(381..914)
                     /locus_tag="VQ514_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HET2500675.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(1003..1779)
                     /gene="recX"
                     /locus_tag="VQ514_001957"
     CDS             complement(1003..1779)
                     /gene="recX"
                     /locus_tag="VQ514_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HET2500676.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINANQLSGDKGPYVLTQKLAQKGISKSTIEEILNDFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYFDAKIAFDELDSQVDEETTQELIFKELDK
                     QYTKYARKYEGYKLKQRLTQVLARKGYDFSDIASALREYL"
     gene            1817..>2828
                     /gene="rlmD"
                     /locus_tag="VQ514_001958"
     CDS             1817..>2828
                     /gene="rlmD"
                     /locus_tag="VQ514_001958"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HET2500677.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPE"
BASE COUNT          910 a          503 c          505 g          910 t
ORIGIN      
        1 atttcatttt actatactgt tacgaaatta gatatataaa tgaaaacttg cctcaaggga
       61 ggttttttgc tagtagaaaa gcgatagaca gaaattgttg gactcttaaa aaaatgaagt
      121 tttgctcaaa agagcttctt aaattaaaac gagcaattat cagttcaaat ctgtttcaaa
      181 agaagaacgg ataaattaat cgatgctttg tttttgagtt aggaagttaa gcatgccaaa
      241 ctgagtcgta ggaataaatt tctttataga aatgtgttgt aactctgcta taatggattt
      301 attccttttt gtgtttacac aatttatttt atactaccaa aaaaacaagg tcaggatttt
      361 gttcctgacc tttgacaact ttaccgattc tttagttcta catagcgctt gtaccaaatg
      421 tttacatagg cttctgagaa aggaccacgt ccattgttaa tccaatcaac aagaattttg
      481 acatgttctt ttaaaatata gtccaagtca tcagaataat tcattttgcg tttgtgacgc
      541 tcgtactctt caacgtccaa gagacgtttt tccccatctg taaaaatttt aacatccaaa
      601 tcgtaatcaa tatacttcag tgcttcttca tccagatagt aggggctagc catattgcaa
      661 tagtaagaag ttccattatc acgaatcatg gcaatgatat taaaccaata tttcttgtga
      721 aagtaaacaa tagccggttc tcgagtgacc caacgacgac catcactttc ggtaacaagt
      781 gtatgatcgt tgacaccaat aatggcgttt tctgttgttt ttagtaccat ggtgtcccgc
      841 caagttcggt ggagactccc atcatgctta taactttgaa ttgtaataaa gtcgccttct
      901 tttggaagct tcataactaa ccaactttct acaatttata agtttatcat ttactattgt
      961 accataaaat tacctaaaat ctgtgaattt cacttggaaa tattaaagat attctctaag
     1021 agcgcttgct atatccgaaa aatcgtagcc ctttcgtgct aaaacttgag ttaaacgctg
     1081 cttcagtttg tatccttcat actttcgggc atacttagta tattgcttat caagttcctt
     1141 gaagatgagt tcctgagtcg tttcttcatc aacttgacta tccaattcgt caaaggcaat
     1201 tttagcatca aaataagaga agcccttgtt agtcaagttc tggataatct tatcttgcag
     1261 ggcacgagct ggaagttttc cctcatattt tttcaatagt ttattggcta cacgttgagc
     1321 aacttccgaa aaatcaaaat cattcaagat ttcttctata gtagattttg aaattccttt
     1381 ttgtgctaat ttctgagtca gtacataagg tcccttgtct cctgaaagtt gattggcatt
     1441 gatgatagca taagcgtact gaccatcatt aatccactta tcttctttaa gattagcaat
     1501 aacttgagaa acgatgtttt tatcaatatc gtattttttc agatattctc tgacttcttt
     1561 ttcagtgcgt gctttaaagg ataagtggta gagggccaga ttcttaccat aagaaaattg
     1621 agcaaagtct tgaatctctt tcaattcctc ttcgcttatc accttatctc tcgataacat
     1681 aaaacgaaca attgtatctt cggtgatata gcatttgtcg ccattatcaa gctccatcag
     1741 atagagtctt tttttctttt caagttttgt gattttcata gctctattat aactcaaaat
     1801 gtgataagat aggggtatga atctgaaagt gaaacaaaaa ataccattaa aaatcaagcg
     1861 catgggaatt aacggtgagg gaatcggctt ttaccaaaaa acattagtct ttgtaccagg
     1921 agctctcaaa ggcgaagata tctattgtca gattacttct attagacgca actttgttga
     1981 agcaaaatta ctgaaggtca acaagaagtc taaatttcga attgtgccat cttgtactat
     2041 ttataatgaa tgcggaggct gccaaatcat gcacctgcat tatgataagc agctggagtt
     2101 caagacggac ttacttcatc aagcgctgaa aaaatttgct cctgcaggat atgaaaatta
     2161 tgaaattcgt ccaactattg gaatgcagga accaaaatat tacagagcta agttacaatt
     2221 tcagactcga aaatttaaaa atcaggtcaa ggcgggctta tatgcacaaa actctcacta
     2281 tttagtagag ttgaaagact gcctggtaca agataaggaa acccaagtga ttgctaatcg
     2341 cttagcagaa ttacttactt atcaccagat tccaatcacg gatgagagaa aagttctagg
     2401 tgtccgtact attatggtcc gacgcgcgag aaagaccgga caggttcaga ttattattgt
     2461 tacaaaccgc cagcttaatt taactcaatt ggtaaaagag ttggttaaag atttcccaga
     2521 agttgtgaca gtagctgtta atacaaatac agctaaaacc agtgagatat atggtgaaaa
     2581 gacagagatt atctgggggc aagagagtat tcaagaaggt gtactcaatt atgaattttc
     2641 actatcccct cgagcttttt atcaactaaa tcctgagcaa acagaagtcc tctatagcga
     2701 agcagtaaaa gcgctggatg ttgataaaga agaccatttg attgacgctt attgtggagt
     2761 tggaacgatt ggatttgcct ttgcaaagaa agtaaaaaca ctcagaggta tggatattat
     2821 tccagaag
//
LOCUS       DAVBCW010000150         2820 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.150, whole genome shotgun sequence.
ACCESSION   DAVBCW010000150 DAVBCW010000000
VERSION     DAVBCW010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.150"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            52..693
                     /locus_tag="VQ514_001959"
     CDS             52..693
                     /locus_tag="VQ514_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HET2500678.1"
                     /translation="MRAGVLDANDGIISIAGVVIGVASATTNIWIIFLSGFAAILAGA
                     FSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYAAYIQNEEFKTSAQLLT
                     NKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSDYRI
                     PATVLIVGVALLLTGYTSARLGKAPTKTAMIRNLAIGLLTMGVTFLLEQLFSI"
     gene            complement(735..2723)
                     /locus_tag="VQ514_001960"
     CDS             complement(735..2723)
                     /locus_tag="VQ514_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2500679.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTVTYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVVFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQTKVVITVLV
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSSESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYSVKEKEVLRYSNFGIANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNEGVKEQKALTLNDHQFSVKEEFTKDFIVNHVPNQF
                     NILTADYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVNASEAEQLKVAEEYEKYLQKF
                     NAQLNTEGNYVYGSTLADASAQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFLHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(2725..>2820)
                     /locus_tag="VQ514_001961"
     CDS             complement(2725..>2820)
                     /locus_tag="VQ514_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2500680.1"
                     /translation="LYNQIYRGEKTERQMFQEISDTLTVMASEVN"
BASE COUNT          871 a          564 c          552 g          833 t
ORIGIN      
        1 tcagaaacaa atcacgaaat tgattcaaat tttgcaggtc gtttaaatat cctgcgtgcg
       61 ggtgttcttg atgctaacga tggaattatt tccattgctg gtgtggttat cggggttgcc
      121 agtgccacga ccaatatctg gattatcttt ttatcaggat ttgcggctat cttagctggt
      181 gccttttcaa tggctggtgg agaatatgta tccgtttcaa ctccaaaaga taccgaggaa
      241 gctgccgttt cgcgagaaaa actcttgcta gaccaagata gggaactagc caaaaaatcc
      301 ctctatgctg cttatatcca aaatgaagaa ttcaaaactt ctgcccaact cttgactaat
      361 aagatctttc ttaaaaatcc actcaaggct ctggtagagg aaaaatatgg gattgagtat
      421 gaagaattta ccaatccttg gcacgctgcc atttctagct tcgttgcctt tttccttaga
      481 agtttgcctc caatgctgtc agtgaccatt tttccaagtg attaccgcat ccctgctacc
      541 gtccttattg tcggtgtggc ccttcttctc actggttaca caagtgctag acttggaaaa
      601 gccccaacca aaacagctat gattcggaac cttgctattg gtctcttgac catgggagtt
      661 accttcctgc tcgaacaact tttcagcatt tagaatacaa gaaatacctc gattttgaag
      721 tcgaggtatc ttttttacat ttgcacaatc ttgcgataac ttcttgaagt aatcatgaaa
      781 atcagcacat aggcgatgag gaagatagcg cagatagaca aggtcacaat caacatcata
      841 gtcgtatcca gtacaccaat cacttttaaa atcagactaa gcatatggta ggcaaaggct
      901 agatgtagga aggcaaagag caaaggaagg aagaatacag ttaaaacctg tttgttgatg
      961 gtttgcttga tttgcttttg atctaaaccg actttctgca agataataaa gcgctcacgg
     1021 tcttcatatc cttcagaaat ttgtttgtag tagatgacca gaacggttcc gaccataaag
     1081 ataatggata agaaaatacc gataaagaag acaccaccaa agagggcact catctgagca
     1141 ctagcatctg ctagagtgct accatacaca tagttacctt cagtgtttaa ttgagcatta
     1201 aacttttgta agtatttttc atactcctca gcgaccttga gttgttctgc ttcactggca
     1261 tttacattca taccaccgta aaactgatta tagatagccg aatctgggaa ttgatccaaa
     1321 aaggcttgta aatcaggtac aacaaggtaa ttgtaatcag cagtcaaaat attaaactga
     1381 tttggaacat ggttgacaat aaaatcttta gtaaattctt cttttacaga aaattgatga
     1441 tcatttagag ttagagcttt ctgttcttta actccctcat tctttgcaaa gagtccgacc
     1501 tcatttcctg atagagacag tttttgacca gtcatatttt cataatcttt ttggtcaaat
     1561 accatgaaaa ctgtttttgg ttggacacgg ttttgtcctt tttcaaaaat agttaactta
     1621 gttccttctt gatttgcaat accaaagtta ctgtaacgaa gtacttcttt ctctttgaca
     1681 ctataacctt tgtcacttgc aaactggctc aagagtttgt ccaagtcttc tttttcaaca
     1741 ttttgccctg aaaccccaaa atcatgagga tttagaactt ttttaaagct ttctgaggaa
     1801 ttgaaaatgc ttgtcgctgc tgacatggtt accaaaacca ttgttgacaa gatagcgatg
     1861 gtggctagtc caactgcatt tttcttcata cggaaaatca agttagaaac agatatgaga
     1921 ttatttggtt ggtaatagta tttcttattt ttctttaaga tttggaggaa aacggtaatc
     1981 cctgcattaa acaagagata agtcccaaag ataaccagta aaacagctat gaagaaggtt
     2041 gttaaggctg taagaggatc ttttaccgta agggcaagat aatagccaat tcctaaactt
     2101 atggaaccaa gaatggtttg gagaggaagg aagcgacctt ttttctctcc actagctttc
     2161 tcacgagaga gctggagggc attcatacgg gcgattcgaa gggcattcag gaacatgagg
     2221 cctaggaaaa tcaaaccgaa gacaacaagc actgtaatga caactttcgt ctggaaggta
     2281 gcaaccagct caaccttcaa tttcattagt ttgagcagga aagcgaaaat taacttgtca
     2341 aacaaggctc caataccgat acccgctcca acagttagaa tcccaaatac cactaactcc
     2401 ttaaaggtca tactgataag atgacgcttc tccaagccca acatgccata aattcctagt
     2461 tccttggaac ggttcttcat gacaaaacta ttggcataga gaacgataat ggctgacgca
     2521 agggtgacga caaacatacc aaatccaagt gtagcctgaa tggttgttcc tccacgaatt
     2581 tcagcaatct tgggattgaa ggttagagag taaaagagat aggtgacagt gactgccaag
     2641 agaacagcca gcgcaaaagg atagtagagt ttgcggtttt taatcaagtt cgataccgct
     2701 aacttattgg ttaatcgaaa catactaatt cacctcgctt gccatgacag tcaaggtatc
     2761 agagatttct tggaacatct gacgttctgt cttctctcca cggtagattt gattgtaaag
//
LOCUS       DAVBCW010000151         2802 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.151, whole genome shotgun sequence.
ACCESSION   DAVBCW010000151 DAVBCW010000000
VERSION     DAVBCW010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.151"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(304..1614)
                     /locus_tag="VQ514_001962"
     CDS             complement(304..1614)
                     /locus_tag="VQ514_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HET2500681.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNFAITNGTLDVLAQHILYRTRTYPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYRKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTSIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            1851..2723
                     /locus_tag="VQ514_001963"
     CDS             1851..2723
                     /locus_tag="VQ514_001963"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HET2500682.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAILLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
BASE COUNT          841 a          488 c          652 g          821 t
ORIGIN      
        1 tcctgatcta tttgtcctgt tcttatttca tttcactata tctcaaattg agtatgacga
       61 agtgcgctcc catgtcctgg gaacgcactt tcttcatatt tttcatattc ttgaatccat
      121 cgataaagac tattgggatg aatttttaaa gttgaactaa tcatttttac aggatgaggt
      181 ttacagcaga gagtttgaag gctttatcaa aggtttttct tggcataatg acttttcctc
      241 gtttccactt aattttgtgt ctactttatt ataccaagtc cacccttaag ttagataata
      301 aatctaactt aaggaagcta gaaggatgag aatccaggtg gtcaagagtc ccaaacttaa
      361 gctgattggg acacccagaa taatttgctt tttgaaggca aggccacgtt cctctatatt
      421 gggaagtgag agttgaatga gagaaccagc tgatgaaaag ggtgagatat tagtagatag
      481 agcgccaata acggtggctg ttgtgagtaa gtgaatatca atctggggat tttgagcact
      541 gatgatagca atgatgggaa agagggctgg agctacaacg gatagggtgg aactaaagag
      601 tgacatcacc ccggctatca cacaaaagaa cagaggtaac cagaaatgag gaatggatgt
      661 tgtcatgagg tgccctatca gtgtgactaa acctgacttg accgctagag acattagtaa
      721 gctcatgccg cagagcatga taattgtagc ccagggaact ttagccaaaa tggcttcttg
      781 cttccctaat ttgagcctta aggcgaggca gaccatgagt attgagacaa agccaatatc
      841 aaatgttttt cgataagtag ctatccaggc gatgtttggg aaaatgagat gcaacaaggg
      901 aaaaagccaa accaaaacca tgctgctgat catgagcaag gtggtttgtc tttgaacctt
      961 gctgaggagt ggtggttggt caatagtcaa ggatgagttt gttcttccct tactatagtg
     1021 actgtaacag gataataaaa gcaagacaat gagtgggtag ataatgctga cgataaagat
     1081 atgattgcca agtgaaaaag cttgctcttc ccatcccatt tgcttaaaca ggccttgaaa
     1141 gacaatgcct gagctactgg ttatcaaatt agcccctcct gaagctcccc aattgacggc
     1201 ttgagctcca atcaaagggt gtttgtccgc tttttgacag agagtaatcg ctagaggaca
     1261 gcaaacggcc atagtagtga aaaatccagc acctaaagca gacaaaaggg ttgccatcag
     1321 gtataaaatc atgtagaggg cgttagggta ggtgcgtgtg cggtagagaa tgtgttgagc
     1381 caaaacatca agagtaccgt tagttattgc aaagttataa aagagagaga cgctaaaaat
     1441 ggtaaaaaag agtgaggttg gccaaaaatg aagaagttct ttggggctta atcccatgag
     1501 agtggttgcg atgaggtaag aaaaagcaat agccagcagg ccaatattga ttttggtgcg
     1561 gtaaccaatt ccaatggcta gagcaatggc gctaatcatt attaaatgaa tcattgaatt
     1621 gtcctttcag ttagaatatg gaaataggtt ggcaattttg gcgctcaaaa gaccacctgt
     1681 ttcctattgg tacttgacta ttatacacaa aaaaaataca attgtcttga caattgtatt
     1741 gacacttgtt agaattaaaa atgaggtatt caggcttgtg atttcctatg gaagttaata
     1801 gtgattgcct ctaatgctta caagtgatat taaaaataga ggacctagtg atgtcaatca
     1861 tttcaactga tttaacccct tttcaaatag atgatacatt gaaagcagcc ttgcgagaag
     1921 atgttcattc cgaagattac agtaccaatg ccatttttga tcatcatggc caagccaagg
     1981 tgtcgctttt tgccaaggaa gctggtgttt tagcggggct aaccgttttt caaagggttt
     2041 ttaccctatt tgatgccgag gtgaccttcc agaatcctca tcaatttaag gatggggatc
     2101 gtttgactag tggcgatttg gttttagaaa tcataggctc ggtgagaagt ctcttaacat
     2161 gtgaacgcgt tgccttgaat tttttacaac atttatcagg gattgcttcg atgacagctg
     2221 cttatgtaga agccttaggc gatgattgca ttaaggtatt tgatactcga aaaactactc
     2281 ctaatttacg tctttttgag aaatatgcag tgagagttgg cggtggctat aatcatcgct
     2341 ttaatttatc agatgctatc ctgctaaaag acaatcacat tgcggcagta ggtagtgttc
     2401 aaaaggcaat tgctcaagcg cgtgcctatg ccccttttgt gaaaatggtc gaggtggaag
     2461 tggaaagcct tgctgctgcc gaagaagctg cggcggcggg tgctgatatt atcatgttgg
     2521 ataatatgtc attggaacag attgaacagg ccattaccct aattgcagga cgttctcgga
     2581 ttgaatgttc tggaaatatt gatatgacca ctattagccg ttttcgtggt ttagcgattg
     2641 attacgtctc cagtggtagt ttaacccata gtgctaagag tcttgatttt tccatgaagg
     2701 ggttaaccta ccttgatgtc taagttgtaa aataaactaa ctttttaaag gatgtctttc
     2761 ctctagaacg agttttatgt cagatagttt aaacgcctct tc
//
LOCUS       DAVBCW010000152         2639 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.152, whole genome shotgun sequence.
ACCESSION   DAVBCW010000152 DAVBCW010000000
VERSION     DAVBCW010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.152"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            200..2359
                     /gene="pfbA"
                     /locus_tag="VQ514_001964"
     CDS             200..2359
                     /gene="pfbA"
                     /locus_tag="VQ514_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HET2500683.1"
                     /translation="MLKIVKKLEVLMKYFVPNEVFSIRKLKVGTCSVLLAISILGSQG
                     ILSDEVVTSSSPMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNS
                     ISSNGTIRSNSQLDNRTVESTVISTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAV
                     GDGIHDDRQAIQDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILN
                     GINIKNHPSIVFMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLI
                     NSSGAFAIGNSNNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQ
                     IISKESIQIEPLTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTL
                     STQNPSNIKILNNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVN
                     AYSYKNTKDLLDLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVSDITVTKNVIN
                     NNSKETEQPNIELLRVSDNLVVSENSIFGGKEGIVIEDSKGKITVLNNQFYNLSGKYI
                     SFIKSNANGKESVIRDSDGNFNIVTENGLYKIVTNNLSDKNEKEKNKEEKQSNSNNVI
                     DSNQKNGEFNSSKDNRQMNDKIDNKQDNKTEEVNYKIVGDGRETENHINKSKEIVDVK
                     QKLPKTGSNKIMELFLTVTGIGLLLTLKGLKYYGKDK"
     gene            2343..2639
                     /locus_tag="VQ514_001965"
     CDS             2343..2639
                     /locus_tag="VQ514_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500684.1"
                     /translation="MVKINKICSIQGSSVENEDIVGSQNQYFWIIGGATDLYNSKEEI
                     GYSVSEVVHILSESLSVNCKESKTLKQIFETALLEVKDEIGLNSYELTEYSKMK"
BASE COUNT         1028 a          324 c          497 g          790 t
ORIGIN      
        1 ttcagtctac tatagaactg accaagtcag taacctagac ttagggcaag gcggcactga
       61 cctagtttga agagatttcc gaagagtata aattttaata ttttcttgtg ttattccttg
      121 acaattcaat ttggaaaata tatgataaag ataatgacag cggtgtcatt ctatctattt
      181 taagaaaagt aataatcaat tgttaaaaat agtaaaaaaa ttggaggttc tgatgaaata
      241 ttttgttcct aatgaggtat tcagtattcg taaattaaag gtggggactt gctcggtact
      301 attggcaatt tcaattttgg gaagccaagg tattttatcg gatgaagttg ttactagttc
      361 ttcaccgatg gctacaaaag agtcttctaa tgcaatcact aatgatttag ataattcacc
      421 aactgttaat caaaatcgtt ctgctgaaat gattgcctct aattcaacca ctaatggttt
      481 agataattcg ttaagtgtta atagtatcag ctctaatggt actattcgtt ccaattcaca
      541 attagacaac agaacagttg aatctacagt aatatctact aatgaaaata agagttataa
      601 ggaagatgtt ataagtgaca gaattatcaa aaaagaattt gaagatactg ctttaagtgt
      661 aaaagattat ggtgcggtag gtgatgggat tcatgatgat cgacaagcaa ttcaagatgc
      721 aatagatgct gcagctcaag ggctaggtgg aggaaatgta tattttcctg aaggaactta
      781 tttagtaaaa gaaattgttt ttttaaaaag tcatacacac ttagaattga atgagaaagc
      841 tacaattcta aatggtataa atattaagaa tcacccttcc attgttttta tgacaggttt
      901 atttacggat gatggtgcgc aagtagaatg gggcccaaca gaagatatta gttattctgg
      961 tggtacgatt gatatgaacg gtgctttgaa tgaagaagga actaaagcaa aaaatctacc
     1021 acttataaat tcttcaggtg catttgctat tgggaattca aataacgtaa ctataaaaaa
     1081 tgtaacattc aaggatagtt atcaagggca tgctattcaa attgcaggtt cgaaaaatgt
     1141 attagttgat aattctcgtt ttcttgggca agccttaccc aaaacgatga aggatgggca
     1201 aatcataagt aaggagagca ttcagattga accattaact agaaaaggtt ttccttatgc
     1261 cttgaatgat gatgggaaaa aatctgaaaa tgtgactatt caaaattcct attttggcaa
     1321 aagtgataaa tctggggaat tagtaacagc aattggcaca cactatcaaa cattgtcgac
     1381 acagaacccc tctaatatta aaattttaaa taatcatttt gataacatga tgtatgcagg
     1441 tgtacgtttt acaggattca ctgatgtatt aatcaaagga aatcgctttg ataagaaagt
     1501 taaaggagag agtgtacatt atcgagaaag cggagcagct ttagtaaatg cttatagcta
     1561 taaaaacact aaagacctat tagatttaaa taaacaggtg gttatcgccg aaaatatatt
     1621 taatattgcc gatcctaaaa caaaagcgat acgagttgca aaagatagtg cagaatattt
     1681 aggaaaagta tcagatatta ctgtaacaaa aaatgtaatt aataataatt ctaaggaaac
     1741 agaacaacca aatattgaat tattacgagt tagtgataat ttagtagtct cagagaatag
     1801 tatattcggt ggtaaagaag gaattgttat tgaggattca aagggtaaaa taaccgtttt
     1861 aaataaccaa ttttataatt tatccggtaa gtatatatca ttcatcaaat ctaatgcaaa
     1921 tgggaaagaa tctgttatac gtgatagcga tggtaatttc aatattgtaa cggagaatgg
     1981 gctttacaaa attgtaacaa ataatttaag tgataaaaac gaaaaagaaa aaaacaaaga
     2041 ggaaaaacaa tctaattcaa ataatgtaat tgatagtaac cagaagaacg gagagtttaa
     2101 ctcaagtaaa gataatagac aaatgaatga caagatcgac aataaacaag ataataagac
     2161 agaagaagta aactataaaa tagttggaga tggcagagaa actgaaaatc atattaataa
     2221 atctaaagaa atagtagatg taaaacaaaa attaccaaag actggttcga acaagattat
     2281 ggaactattc ttaacagtga caggaattgg tttacttttg acactaaaag ggttgaagta
     2341 ttatggtaaa gataaataaa atttgttcga tacaaggaag ttccgtagaa aatgaggata
     2401 ttgtaggttc acaaaatcag tatttttgga ttattggtgg agctacagat ttatataatt
     2461 ctaaagagga gataggttat tcagtctcag aagttgtaca tattttatca gaaagtttgt
     2521 cggttaattg taaagaatca aaaactctta aacaaatatt tgaaactgct ttactcgagg
     2581 ttaaagatga aatcggttta aactcatatg aattaacaga gtatagtaaa atgaaataa
//
LOCUS       DAVBCW010000153         2637 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.153, whole genome shotgun sequence.
ACCESSION   DAVBCW010000153 DAVBCW010000000
VERSION     DAVBCW010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.153"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(560..1429)
                     /locus_tag="VQ514_001966"
     CDS             complement(560..1429)
                     /locus_tag="VQ514_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HET2500685.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTEKPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
     gene            complement(1652..2275)
                     /locus_tag="VQ514_001967"
     CDS             complement(1652..2275)
                     /locus_tag="VQ514_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET2500686.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVISGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPEKPIPQPNPEHPSVPTPNPELPNQETPTPDKPTPEPGTPKAETPVNPDPE
                     VPTYETGKREELPNTGTEANATLASAGIMTLLAGLGLGFFKKKEDEK"
     gene            complement(2233..>2637)
                     /locus_tag="VQ514_001968"
     CDS             complement(2233..>2637)
                     /locus_tag="VQ514_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HET2500687.1"
                     /translation="TFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANYYK
                     EGSQEKLAESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPANA
                     YQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
BASE COUNT          733 a          550 c          508 g          846 t
ORIGIN      
        1 ctcaaaactg tgttttgaac aacctgcggc tagcttctta gtttgccctt tgattttcat
       61 tgagtataaa attgcagata aaacgataac aatcatagga ctcagcagag tcaatgtagg
      121 tcgaagaata gcccccctaa tcgcttattt tatagcaaaa tgaaatagaa actgaacatt
      181 ttgttgagga aggtaaatca attcctcaca atgtttgaga aagaagggag gactattcca
      241 acttcaaccc actataaagc agacttacaa gatagggaac ccatctccga taatatatag
      301 tagaatgaaa actgcacaat ttgattggag aattcaaacc aatttctaac aatgttttag
      361 aaacaagggt ctattatttc aactgcaacc tactatagcc cattgaagct ggaatagccc
      421 actgagactt ctaaaccact gctggagagg agtttgttta gagtttcttt catttcccta
      481 aaaaatcaaa aagagagaac cagtctgatc ctctcttact tatagatggt gctcactcaa
      541 atgttaaagg agctatttct tagagataaa ggaatttgaa tacagctact gccgcaattg
      601 ctgctgcgat aggtgctact actggtaccc aagaatacca ccattttgaa tcgcctttat
      661 gctcaccaag aactgatttt ggaaggaaag catgaaggag acgtggtccc aagtcacggg
      721 ctgggttcaa ggcaggtcct gtaggtcctc caagtgatgt taccaaagcc ataacgagga
      781 atccaagtgc caagtgagcc acagaaagtc ctgaagcagt gtgtggagcc acctgtgctt
      841 taatagccaa gtcagaaaaa tcaactgttt gtcctgcttc tattgccttt tgtttcatga
      901 attgaagcac ttcagcacca aagaagtttt tagtcaaacc aagagctgca aagaacaaaa
      961 caaatgaacc aacaaactca ttaatcaaac cattgacagt tgctgcatag cgactttctt
     1021 ttgtaccatg gtcaatactt gaaatagttg agaaagttcc caagatgtta tttggttttt
     1081 cagttttcaa gtagaatgga cggtatgttg ccacaactaa ggcttggcca aagatagccc
     1141 ccaagacttg cgcgataatg taaggtacca cttgtgccca agggaaaaga ccgctaactg
     1201 caagccctag agtgaaagca gggttgatgt gattcccaga tacgttacca aacatcaagg
     1261 ctgggatcat aacccccata ccataaccaa cagcgatgac gatccagcca ctttggtgac
     1321 ctttcgtacc tttaagttca acgttggcaa ctgcaccatt cccaagaatg atcaaaatgg
     1381 cagttcccaa aaattcagtg gcatacttca gtgcccatgt gaaatccatt tgctagattc
     1441 tccttaaaat tttttagata ttccttattt tatcaatttt taccctattt agcaactgat
     1501 ttctggaaat atttttaaaa aaataggatt catagtttcc cagttcaacg tacttgtaag
     1561 cgtttttatt cttcacgaaa gacctcattc tcaaaaccag gaaaaagccc acatctgtga
     1621 tcttttcctg gttcctagat tctaaaatct attatttttc atcttctttt ttcttgaaga
     1681 atcctaatcc tagaccagct aacaaggtca tgataccagc actagccaag gtagcattag
     1741 cttctgtacc tgtgtttggc aattcctctc tcttacctgt ctcataagtc ggaacttctg
     1801 ggtctggatt cactggagtt tcagcttttg gagtacctgg ttctggagtt ggtttatctg
     1861 gtgttggagt ctcttgattt ggtagttctg ggtttggtgt cggaacactt ggatgctctg
     1921 gatttggttg tgggattggt ttttctggag ttggtttatc tggtgttgat aaacggtcat
     1981 accttaccgt tatttcttta tcactagagt ctgacgtaac ttcttgtgat tcaactgttg
     2041 gaatatctgg atctttgtac ttgtcaatct taccagatat aacctcgtcc cagtttcctg
     2101 ttgtccattc accgtaggtt acaactcccg tgaccttgtt ctcagttttt gtacggctta
     2161 aggttacagg ttgaacaaca tcttctttta cattttggtt cgtaacttta tcaacgtaat
     2221 gaatgatacg cgttatagtc ttcgtctcag tagaggttgc tgttttggga accactgttt
     2281 cctcaacatt ctcacggtag taatagtcaa ctgttgcacc gtcttctggt acgcatttgc
     2341 aggagttgct accaaggtgt atgttgtttt ccttgtgata actcggtctt ctttgtcctc
     2401 agttgttgtt ttcccttcaa tagtttttga ttctgtggta tactcagaac ctatcgctaa
     2461 atcagctttt ataacagact ctgccaactt ctcttggcta ccttctttat agtaattggc
     2521 tgttactgta gcagtggttg gcgcttcgct ttctctataa actaaggtca ctgttctacc
     2581 ttcgcttaca atattcccag ttaaacttgc agaatttgta tctgcttctt taaaagt
//
LOCUS       DAVBCW010000154         2621 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.154, whole genome shotgun sequence.
ACCESSION   DAVBCW010000154 DAVBCW010000000
VERSION     DAVBCW010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.154"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(478..703)
                     /locus_tag="VQ514_001969"
                     /pseudo
     CDS             complement(478..703)
                     /locus_tag="VQ514_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1551..2423
                     /locus_tag="VQ514_001970"
     CDS             1551..2423
                     /locus_tag="VQ514_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HET2500688.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
BASE COUNT          779 a          453 c          510 g          879 t
ORIGIN      
        1 agatatgcac aaattgatta ggaaattcaa atgaatttct acaaatgttt tagcaatcgt
       61 aatgtacttg tctagattcg atctgatata ttttcgattt aatgatatgg tatttaaaac
      121 ctccaaagta gcttgctcca ttcttttact tacgtgagtg tagatgttat ttactgtttt
      181 agcgtttttg tgttccactc taaccattat agcattcttc tcagctagtg tactaaggag
      241 tgtgtgcctg aaaatatggg aactaagggg ctggtttatc ggtttctcta gtttagtatt
      301 tgccttttgc aaagtgatgt taaatgcctt tctctaaatt tacatatcac tattgtttaa
      361 caaaatctaa tctactttag gtcacttatt ctttttttga aatgtagaat gaactttttc
      421 aaagtttttc gaatctttta aaatctgttt gttttatatc gccattctcc ccctttttta
      481 attctcccta tatagcctga caactttccc gatggtacga atatggttgc tttcgtctag
      541 gtggatgtcg gggtatttgg gattgagttt ttttaaggta gccttggcgg agtttcttga
      601 catagttagc gccgtctact tggaagatgc cgatggtatt atagtcaatc tgtggggtat
      661 tcttgataaa taggtagtcg ctgtttctta tctttggctc catggacttg ctgacgacat
      721 aagcgattgg gtcgtagtcg tctgggataa tggaaactcc ataactaaat cgtcgtcctg
      781 catcgagcgg ctacctgcag agataaacta cctaacacga gagtaagtag tctgtctgta
      841 gtcgtccagt ctgatgattt ttacgatatt tcgtttttct gctcatacag ttgcctctcg
      901 gcataggtca gaactttacc ttgtctgggt ggtgcccgtt ggtcgtagat agattggata
      961 tcgctaggag aatccttttg aactggagga aagagggtat caatcaagct actgaatact
     1021 ttagctaagt caaatatagt atttttctta gtagacctaa cccttttttc ataatttcca
     1081 atggtgtttt tacttatacc aatcttagta cccaattctt attgagtcca accattacta
     1141 gtctatattg ttttatagtt gattgagttt ggaatagtac gctgtagctg ctaaaacatt
     1201 tctaggaatt aatttgactt tcctaataga gttgttcata tcttatttca atctactatg
     1261 tttttcacct ctaacaatcg caatctcttc tttatccatg aatgaaatcg ctttctattt
     1321 ttgtaagtaa agcatcacac gaaatccacg aaaatgaaaa cctttgttgt gttttcgtaa
     1381 aaaatttgtt gacagagcac gaaacgccta ctataattaa atcaagctta aggaaataac
     1441 aaaaaaacaa atcggaggga aagacgatga acacattaaa tgagaaagta atcaatatct
     1501 gtaaagcagt agttaaagaa actttaatcc aagacattta ggagaaagaa atgaagaaat
     1561 caagaaaatt agccacatta ggaatctgtt cagccttgtt tttaggcttg gctgcttgtc
     1621 aacaacaaca tgctacttct gaggggacga atcaaaggca aagcagttca gcgaaagttc
     1681 catggaaagc ttcatacacc aacctaaaca accaggtaag tacagaagag gtcaaatctc
     1741 tcttatcagc tcacttggat ccaaatagtg ttgatgcatt ttttaatctc gttaatgact
     1801 ataataccat tgtcggctca actggcttat caggagattt cacttccttt actcacaccg
     1861 aatacgatgt tgagaaaatc agtcatctct ggaatcaaaa gaagggcgat ttcgttggga
     1921 ccaactgccg tatcaatagt tattgtcttt tgaaaaattc agtcaccatt ccaaagcttg
     1981 aaaagaatga ccaattgctt ttcctagata acgatgcgat tgataaagga aaggtctttg
     2041 attcacaaga taaggaagag tttgatattc tattttcgag agttccaact gaggcaacta
     2101 cagatgtcaa ggttcacgct gaaaagatgg aaacattctt ctcacaattt caattcaatg
     2161 aaaaagctcg aatgctgtct gtagtcttgc acgacaatct ggatggcgag tatctgtttg
     2221 taggccatgt tggggtctta gtacctgctg atgacggttt cttatttgta gagaaattga
     2281 ctttcgaaga gccctaccaa gcgattaaat ttgctagtaa ggaagattgc tacaagtatt
     2341 tgggcaccaa gtatgcggat tatacaggcg agggactggc taagcctttt atcatggata
     2401 atgataagtg ggttaaactt taatagtgtt atgatatgac agtaatctta ggatttttat
     2461 ttctattttt gatgacttgg tgggttattt caatcgttaa tagccagaaa ccacgcaaaa
     2521 atgaaacatt tattggctat atccaaagat acgatattga tgggaatggc tatcaatcat
     2581 taccgtgtcc tcagaactga gaggagttct tgaaaccgta a
//
LOCUS       DAVBCW010000155         2605 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.155, whole genome shotgun sequence.
ACCESSION   DAVBCW010000155 DAVBCW010000000
VERSION     DAVBCW010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.155"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            412..2517
                     /locus_tag="VQ514_001971"
     CDS             412..2517
                     /locus_tag="VQ514_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET2500689.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTSEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSQLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMKDGVLVIREKA"
BASE COUNT          840 a          469 c          605 g          691 t
ORIGIN      
        1 tatatagttt tgagatggtt gatagaactg acgaagtcag ctcaaaacat ggttttgagg
       61 ttgtagataa ggagaagctg atgtggtttg aagaggtttt cgaagagtgt aaaaaaatat
      121 tggtcagtta aagccaaaac tcttgacctt ttctgaccaa tgtagtatat tatgaacgta
      181 aggtaaatga aagccttact agaacactta tttaaagtaa aatagaaaga ggtgggtcta
      241 tgtttcatct agaaatcttc agaagtaaag atggtctact cctgcttgaa aaagaaaaac
      301 cagaaatagt acgtagggta gcggtttaac tagtctaaat tttttaaaac aatggtcaaa
      361 gatagtcaat atcagtaatc ataactaagt aaacaaaaag aggtaaagaa tatgaataac
      421 aactttaata attttaataa catggatgat ttatttaacc aattgatggg tggtatgcga
      481 ggatatagtt ctgaaaatcg ccgttactta attaatggac gcgaagtcac atctgaggaa
      541 tttgctcact atcgtgcgac tggtcaatta ccaggaaatg cagaaactga tgtgcaaatg
      601 ccacaacagg catcaggtat gaaacaagac ggtgtccttg caaaactagg tcgaaacttg
      661 acagcagaag cgcgtgaggg caagttggat cctgttatcg gacgaaacaa ggaaattcaa
      721 gaaacatctg aaatcctctc acgccgcacc aagaacaatc ctgttttggt cggagatgca
      781 ggtgttggta agacagcagt tgtcgaaggt ctagcgcaag ccattgtgaa cggagatgtt
      841 cctgctgcta tcaagaacaa ggaaattatt tctattgata tctcaggtct tgaggctggt
      901 actcaatacc gtggtagctt tgaagaaaat gtccaaaact tagtcaatga agtgaaagaa
      961 gcagggaata ttatcctctt ctttgatgaa attcaccaaa ttcttggtgc tggtagcact
     1021 ggtggagaca gtggttctaa aggacttgcg gatattctca agccagctct ctctcgtgga
     1081 gaattgacag tgattggggc aacaactcaa gacgaatacc gtaacaccat cttgaagaat
     1141 gctgctcttg ctcgtcgttt caacgaagtg aaggtcaatg ctccttcagc agagaatact
     1201 tttaaaattc ttcaaggcat tcgtgacctc tatcaacaac atcacaatgt catcttgcca
     1261 gacgaagtct tgaaagcagc ggtggattat tctgttcaat acattcctca acgtagcttg
     1321 ccagataagg ctattgacct tgtcgatgta acagctgctc acttggcggc tcaacatcca
     1381 gtaacagatg tgcatgctgt tgaacgagaa atcgaaacgg aaaaagacaa gcaagaaaaa
     1441 gcagttgaag cagaagattt tgaagcagct ctaaactata aaacacgcat tgcagaattg
     1501 gaaaagaaaa tcgaaaacca cacagaagat atgaaagtga ctgcaagtgt caacgatgtg
     1561 gctgaatctg tggaacgaat gacaggtatc ccagtatcgc aaatgggagc ttcagatatc
     1621 gaacgtttga aagatatggc tcatcgcttg caagacaagg tgattggtca agataaggcc
     1681 gtagaagctg tagctcgtgc tatccgtcgt aaccgtgctg gttttgatga aggaaatcgc
     1741 ccaatcggca gcttcctctt tgtagggtct actggggttg gtaagacgga gcttgctaag
     1801 caattggcac tcgatatgtt tggaacccag gatgcgatta tccgtttgga tatgtctgaa
     1861 tacagtgacc gcacagctgt ttctaagcta attggtacaa cagcaggcta tgtgggttat
     1921 gatgacaata gcaatacctt aacagaacgt gttcgtcgca atccatactc tatcattctc
     1981 ttggatgaaa ttgaaaaggc tgatcctcaa gttattaccc ttctcctcca agttctagat
     2041 gatggtcgtt tgacagatgg tcaaggaaat acagtaaact tcaagaacac tgtcattatt
     2101 gcgacctcaa atgctggatt tggctatgaa gccaacttga cagaagatgc ggataaacca
     2161 gaattgatgg accgtttgaa acccttcttc cgtccagaat tcctcaaccg ctttaatgca
     2221 gtcatcgagt tctcacaatt gactaaggaa gacctttcta agattgtaga tttgatgttg
     2281 gctgaagtta accaaacctt ggctaagaaa gacattgact tggtagtcag tcaagcggct
     2341 aaagattata tcacagaaga aggttacgac gaagtcatgg gggttcgtcc tctccgtcgc
     2401 gtggttgaac aagaaattcg tgataaggtg acagacttcc acttggatca tttagatgct
     2461 aaacatctgg aagcagatat gaaagatggc gttttggtta ttcgtgagaa agcctaagac
     2521 agaattttga ggataaaaaa gaaggagcca gctgaaaaaa ctggttcctt ttgaggtacg
     2581 acgggcatgt cgtatagtag aagtg
//
LOCUS       DAVBCW010000156         2574 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.156, whole genome shotgun sequence.
ACCESSION   DAVBCW010000156 DAVBCW010000000
VERSION     DAVBCW010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.156"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            190..504
                     /locus_tag="VQ514_001972"
     CDS             190..504
                     /locus_tag="VQ514_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500690.1"
                     /translation="MWVLVFILFMIFFYSNNSKKIKKLENKIKKLERKEKGNAEMSRL
                     LQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRRVDNTGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            514..720
                     /locus_tag="VQ514_001973"
     CDS             514..720
                     /locus_tag="VQ514_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2500691.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            720..917
                     /locus_tag="VQ514_001974"
     CDS             720..917
                     /locus_tag="VQ514_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500692.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            920..1642
                     /locus_tag="VQ514_001975"
     CDS             920..1642
                     /locus_tag="VQ514_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HET2500693.1"
                     /translation="MKKKRRLLFLMSIVLGMFVGMFKARVESHEIILDVKALMPWISA
                     ICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAFNITG
                     VAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLEFFAT
                     PKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQLLAFL
                     LVGAIHVYINVMQLPMVKRYFK"
     gene            1654..2295
                     /locus_tag="VQ514_001976"
     CDS             1654..2295
                     /locus_tag="VQ514_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2500694.1"
                     /translation="MKLLKNLGWILLALLSFLFIYGFIQGLATASLALGASPYAVTLL
                     YVALAGVYVYGIYKWYQKAPVHIEKSGFNRFIWLPVLVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            2313..2543
                     /locus_tag="VQ514_001977"
     CDS             2313..2543
                     /locus_tag="VQ514_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500695.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
BASE COUNT          754 a          424 c          521 g          875 t
ORIGIN      
        1 gctgacgtgg tttgaagaga ttttcgaaga gtattaatga tagataaact ggtcagtaac
       61 ctactcctga cctttttctt ttgcaaaatg acaaaaatca taaacttttt gacaactata
      121 cttgtcaaaa agaaaaagat gtgttacaat gaattcaagt taagaaatag gaagcagaaa
      181 ggagttataa tgtgggtact agtatttata ctatttatga ttttctttta ttctaataat
      241 tctaaaaaaa tcaagaaact agagaataaa atcaaaaaac ttgagcgaaa agagaaagga
      301 aacgcagaaa tgtcgagatt attgcaagaa atgattggaa aggaaccaat tataacggga
      361 gtgtatattg ggccagataa ctgggaagtt gtggatgttg atgaggaatg ggtaaagcta
      421 cgacgtgtag ataatacggg aaaagaaaaa ttcaagttgc aacgcattga ggatatccaa
      481 accgttgaat ttgacggaga gtaggtgtgt aacatgcaac ttaaaaatcg tctaaaagag
      541 cttagagctc gcgatggtct caatcaaacc gacctagcca aactggcagg ggtttccaga
      601 cagaccatta gcctactaga acgggatgag tacaccccat ccattatcat tgccctgaaa
      661 atatctcaaa ttttcaacga aacagtcgaa tcggtatttc gtttggagga ggacgagtga
      721 tgaacaagta taaagtgatt tattatgtag tggtcatagc tttattagtc agtgtatttc
      781 tactgattgg gatggaccta agctggttta atccctatca aagcgaccaa tttgtttggg
      841 tctactttgc tctcatccca gtaattgaat ggattgaaaa gaaatccaaa aatctagcaa
      901 gtgaaaaagg agaatgaata tgaaaaagaa acgtagattg ttatttttga tgtctattgt
      961 cttgggcatg tttgtaggga tgtttaaggc acgtgtcgaa tcccacgaaa ttattttaga
     1021 tgtaaaagcc ttgatgccat ggatatcagc tatttgttta ctgataggtt tcattagtat
     1081 gtttttgact ttcaatttct taaagaaaag cagaaaattt cattccttgt atcaagagga
     1141 aatggatgac gatctgaatg aaacctatta tgtgcaaatg tatcggaatc ttgagtttgg
     1201 aaccattgct tttaatatta caggtgtagc gattccattg gctattttta tttcattaag
     1261 tgaggtgatt atattgcata caaaccctca aacatttttc ctttctttct tactctttgt
     1321 ggtattctta gtcgctcaaa aatctctttt taaaaccatt gcgattgttc gtcagtttga
     1381 tttagaattt ttcgctacac caaaggatgt cttgaactat ataaattctt atgatgaagg
     1441 ggagcgtcag gctaatttgg aacagagttt tcgaatttta ttccaattac accaatatgt
     1501 cttaccagcc ttatatattt ttcttattat catttctttc ttgacaggag agattcagtt
     1561 actagctttc ttgcttgtag gagccatcca tgtttatatc aatgtgatgc agttacctat
     1621 ggtaaaacgt tatttcaaat aaaggagtta tctatgaaat tacttaaaaa ccttggctgg
     1681 attcttctag cccttctatc ctttttattt atctatggct ttattcaggg gctcgcgact
     1741 gcgtcgcttg ctttaggcgc ttcaccctat gctgttactc tactctatgt ggccttggct
     1801 ggagtatatg tgtacggcat ttacaaatgg tatcagaagg ctcctgttca tattgagaag
     1861 agcggcttta atcgatttat ttggcttcct gtcttggttt ggttcttatc tctggtcgtt
     1921 caattcttct tgccagatga tccttcagta aatcagcaaa tagcgacaga cttgaccttg
     1981 tctcaaccac ttttctcatt ctttgctgtg gttatttttg ctcctttgac ggaagagatt
     2041 gtttttagag ggatgttagc acgctatctc tttcctaagc aggacaatag taaacgaacc
     2101 ctgatttttc ttctggtatc tagtcttcta tttgccttga ttcattttcc aggtgatgtg
     2161 caacaatttt ttgtctattt tagccttggt tttagtttgg gtttggctta cattagcaga
     2221 aaaggtctgg tctacagtat ttctcttcac gctttgaata atttagtcgg ctttttgatg
     2281 attctcatgc tataatagag tcaggaggtc acatgaaacg agtaatttta ttagcagtga
     2341 ttcaggcagt cgttctattt ttcatcattg gagcgctagc ttatgccttc aaaggcgatt
     2401 tcttctataa ctatctagca gttgtctttg ctcctattgc aggtgtactg cgttttggga
     2461 cggcttacat aacggaaatt gtcttgcctc gaaaggcagc cgaaatcgct gaaaagcgta
     2521 aagcaggcaa aaattcaaaa taaaagagtc cggtgaagtt catcggattt ttgt
//
LOCUS       DAVBCW010000157         2514 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.157, whole genome shotgun sequence.
ACCESSION   DAVBCW010000157 DAVBCW010000000
VERSION     DAVBCW010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.157"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(173..838)
                     /locus_tag="VQ514_001978"
     CDS             complement(173..838)
                     /locus_tag="VQ514_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HET2500696.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            complement(852..2231)
                     /locus_tag="VQ514_001979"
     CDS             complement(852..2231)
                     /locus_tag="VQ514_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HET2500697.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLI
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
BASE COUNT          822 a          571 c          470 g          651 t
ORIGIN      
        1 cccgcagcta gtttcctagt ttgatctttg attttcattg agtattactc tctcttgtca
       61 cttccttcta ttttaccata aagtccagcc tttgaagaac ttttactaga agacaagggg
      121 cttctgtctc tatttgccat cttgggcatc aaaaaagagg ggtcatccct ctttacgaat
      181 tcaatgctac tagggtatcc aaatactggt tgttgatgac tgccaaaata taggtatctg
      241 ctttcaagag gtcatctggt ccaaattcaa catccaatgg ggaattttcc tgctctcgga
      301 aacccaaaat attcagattg tatttgccac ggaggtctaa tttactcaga ctttgacctg
      361 cccaagactg aggaattttc atctccacga tagacacatt tttatccaac tgaaagacat
      421 caacactatt atggaaaaga atggtctgtg ctagagactg ccccatttca tactctggcg
      481 agataaccga gtcagctcca atcttttcta gcactttctt agcggtctga cttttgacct
      541 tagcaataac agtcggtacc cccaaactct tacagtgcat aaccgcaagc acactcgact
      601 ccagattttc acctgtcgcg actacaacgg tatcgcaggt atcaatccct gctgatctca
      661 ataattcttc atctgtgatg tcaccaatca ctccacgcgc caaaactggc tcaaactgat
      721 tgatgcgctc tgcgtggtca tcaatagcga taatattcat atcctgcttg gctagggcag
      781 ctaggacact gctcccaaaa attcccaagc ccaaaattcc aatcgtacga tctgacatgc
      841 tctttccttt cttaaccaat actaatatct gctttcatat agtgaatcat atctttcttt
      901 tctggatggt aatctgccaa gctaacaaac aaggtcaagg gaccaattcg tcccataaac
      961 ataagtggca tgataacact gagagccaat ttcccaaggt caggagtcag atttgccgtt
     1021 acaccaactg tactaagagc tgaaatggtt tcaaatatga ggtggataaa gggaggattg
     1081 cctttggctg ttatccctag cagaatcaat cctatcaaga agctcatcaa aaagataata
     1141 aagacactaa aggatttttg aaccgttcgc ggcgcaatcg ttcgtctcgc aacattggca
     1201 tgaggcaagc ctagaagctc acttcgtgca aagaccaaga ggacaaaaaa tgtcgtaatc
     1261 ttgagtcccc cagctgttcc tccaggtgcc ccacctagaa acatctgtaa gatataaatc
     1321 aaaagagtca caggatgagc ctgagtataa tctatcgtag aaaagccagc tgttcgcatc
     1381 gtcactgttt gaaaaaagct aactaaaacc ttatcggcaa cagggagatt gccaatcgtt
     1441 ccagcattgt tccactcaag aaagagagta gttgccgttc caaataacaa caaacctata
     1501 gtcaacaata gtacaagctt cgtatgaaag tgcagacgtc cttttttctt tcttcctaca
     1561 tgaccagcca aatcaaacca gaccataaaa ccaaggccgc ctgtaataat caagcctgca
     1621 atcaccagat tgaccagtaa atcggtctga aaagcaaata aactggtgct ccctaaatta
     1681 tcaaaaccgg cattacagaa ggctgagatc gctagaaaaa tggaactaaa aagaccacgt
     1741 ccccagccaa gttgaggaat aaggcgaaaa ctaagcaaaa tagctcccaa gctctcaacc
     1801 aaaaaggtcg tgagaaaaat agaatagaca aactttctca aagatcgagt ttctccataa
     1861 ctaaaactat cctgaatagt tgcacggcta cgaagactaa gcttttgctt gctctggata
     1921 tagaaaaccc caataaaggt catgagccct agaccaccga tctgaatcaa gagcaaacag
     1981 attatttggc cccatatatt ataggtgtga gctactggaa gggttgagag acctgtcaca
     2041 cagactgcag agacagcagt gaaaagatga tcaaaataag tcgctcgtga gctttcaact
     2101 tggacaaagg gcaagctcaa aagaagagag cctagtaaaa tgaccaaggc aaaactcaaa
     2161 aaaatacgac gggctggcga taaacgagat aagcctagct ctattttttc cttagataat
     2221 ttgaataaca tgtttccatt gtaccataaa aagttcgaaa tgacttagat aggataaggc
     2281 aaaatctact aacaccaagc ctaaaaaatg ccaccttttt attgatagct taggcaagaa
     2341 caatacttga tagatgtgac tataggtcaa aaaagcaaga caggagcttt taacgccagc
     2401 tactctatat gcttttcagg agaaaatttc tctttttgat tataggcaat gagaaccaag
     2461 gcatcgatta gaaagctagt cacagattgc tcaaaacact gttttgaggt tgca
//
LOCUS       DAVBCW010000158         2440 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.158, whole genome shotgun sequence.
ACCESSION   DAVBCW010000158 DAVBCW010000000
VERSION     DAVBCW010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.158"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            79..996
                     /locus_tag="VQ514_001980"
                     /pseudo
     CDS             79..996
                     /locus_tag="VQ514_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001822901.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            complement(1036..1980)
                     /gene="manA"
                     /locus_tag="VQ514_001981"
     CDS             complement(1036..1980)
                     /gene="manA"
                     /locus_tag="VQ514_001981"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HET2500698.1"
                     /translation="MSEPLFLQSVMQEKIWGGTKLRDEFGYDIPSEKVGEYWAISAHL
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDALLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTIKADDLRSTILVSNDFFAVYKWEITGKVNFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVESWTLEGQDLELIVSHP"
     gene            complement(2112..2393)
                     /locus_tag="VQ514_001982"
     CDS             complement(2112..2393)
                     /locus_tag="VQ514_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HET2500699.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYKDGVDLLGYTTWG
                     CIDSVSAGTGEMNKRYGFIYVDRDNVGNGTLKRSKKKSFYWYMSFIAMV"
BASE COUNT          631 a          572 c          456 g          781 t
ORIGIN      
        1 atcgtgcatc agttagttgt ttacttgctt catcattcat agaactacta tgccatattt
       61 tgtttcgcag gaagtctgat catttcaaat ccagccattg ctctaggagc tcaagcgacc
      121 tttatcctat tgaatatctt cattgtatca cgtggggttc aaaaagggat tgaaagagct
      181 tcgaaagtca tgatgcccct gctctttatc atctttgtcg tcatcatcga acgctctctc
      241 agtttgccaa atgccatgga aggggttctt tacttcctca aaccagattt ttcaaaactg
      301 actagcgctg gtctcctcta tgctctgaga caatctttct ttgccctctc actaggggtt
      361 acagccatgt tgacctatgc ttcttacttg gacaagaaaa ccaatctggt ccagtcaaga
      421 atctccatcg tagccatgaa tatctcggta tccatcatgg caggtctagc cattttccaa
      481 gctcggtccc ccttcagact gaatattgaa gggggaccga gcttggtctt tatcgtcttg
      541 cctcaactct ttgacaagat gccttttgga accattttct atgtcctctt cctctttgcg
      601 actgtcactt cttctgtcgt tatgctggaa atcaatgtag acaatatcac caatcaggac
      661 aacagcaagc gtgctaaatg gagtgttatt ttaggaattt tgacctttgt ctttggcatt
      721 ccttcagccc tatcttacgg tgtcatggcg gatgttcaca tttttggtaa agccttcttt
      781 gacgctatgg acttcttggt ttccaatctc ctcatgccat ttggagctct cttcctttca
      841 ctttttacag gctatatctt taaaaaggct cttgcaatgg aggaactcca tctcgatgaa
      901 agagcatgga aacaaggact gttccaagtc tggctcttcc ttcttcgttt cgtcattcca
      961 atcatcatcg tggtcttcat cgcccaattt atgtaatcaa aaggacttga gtaatgaact
     1021 caggtccttt cttttttatg gatgactaac aattaattct aaatcttgcc cttctagagt
     1081 ccaagattca acatcacttg gtaggataaa gtgtctccct ttttggattg gatagttttt
     1141 cccgtcaaca gttagttgac cttgaccagc caagacactc aacaagctgt agtcagctgt
     1201 cttttcaaag ttaacttttc cagtaatttc ccatttgtaa actgcgaaga aatcattgga
     1261 tacaaggata gtggaacgta gatcatctgc tttgattgtc actggacggc tatttgcggg
     1321 ttcaccaatg ttcaagacat cgatagattt ttcaaggtga agttcacgta aattgccctt
     1381 gtcatcctta cggtcaaagt catagacacg gtaagtggta tcgctagact gctgggtttc
     1441 aagaatcaag atacccgccc cgatagcgtg catggtaccg cttggtacat agaagaaatc
     1501 tcctgcttta actggcactt ttgtcaacag cgcatcccag ttcttatcct cgatttgctg
     1561 gcggagttct tcttttgact tggcattgtg accgtagata atctctgaac cttcatctgc
     1621 tgcaatgatg taccaacatt ctgtttttcc aagttcgcct tcatgctcga gtccataagc
     1681 atcgtctggg tgaacttgga cactgagcca gtcgttggca tcgaggatct tggtcaaaag
     1741 tggaaataca ggttctggac gattgccaaa taattcacgg tgttccgcat acaaagtagc
     1801 aagatctgtt ccctcgtaac gaccattggc aactttagaa acaccattta gatgagctga
     1861 gatagcccag tactctccga ctttttcact tgggatgtcg tagccaaact catcacgtag
     1921 cttggtacca ccccagattt tttcttgcat aactgattgt aaaaataatg gttctgacat
     1981 cttaatctcc tgtctgattt ttctcccctc attatagcaa aaaaagagtt ccatttgaac
     2041 tcttttttac atcttataaa gcagggagaa gattttataa aaatagtaaa caaatgtgct
     2101 ctactcgatg cttacaccat tgctataaat gacatgtacc aatagaagga cttcttcttg
     2161 ctacgtttga gagtcccatt tcctacatta tctcgatcta catagataaa gccatagcgc
     2221 ttattcattt cccctgtgcc agctgaaacc gaatcgatac agccccatgt cgtataacca
     2281 agcaagtcaa ccccgtcttt gtaaatggca tctcgcatgg ccttgatgtg ggcctctaag
     2341 taagtaatcc gatagtcatc tgctacataa ccattctcat ccggtgtatc catagcaccg
     2401 agtccatttt ctacgataat actatactaa aatcaaaaag
//
LOCUS       DAVBCW010000159         2419 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.159, whole genome shotgun sequence.
ACCESSION   DAVBCW010000159 DAVBCW010000000
VERSION     DAVBCW010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.159"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            <1..1491
                     /gene="phtA"
                     /locus_tag="VQ514_001983"
     CDS             <1..1491
                     /gene="phtA"
                     /locus_tag="VQ514_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HET2500700.1"
                     /translation="ARGVAVPHGDHYHFIPYSQMSELEERIARIIPLRYRSNHWVPDS
                     RPEQPSPQPTPEPSPGPQPAPNLKIDSNSSLVSQLVRKVGEGYVFEEKGISRYVFAKD
                     LPSETVKNLESKLSKQESVSHTLTAKKENVAPRDQEFYDKAYNLLTEAHKALFENKGR
                     NSDFQALDKLLERLNDESTNKEKLVDDLLAFLAPITHPERLGKPNSQIEYTEDEVRIA
                     QLADKYTTSDGYIFDEHDIISDEGDAYVTPHMGHSHWIGKDSLSDKEKVAAQAYTKEK
                     GILPPSPDADVKANPTGDSAAAIYNRVKGEKRIPLVRLPYMVEHTVEVKNGNLIIPHK
                     DHYHNIKFAWFDDHTYKAPNGYTLEDLFATIKYYVEHPDERPHSNDGWGNASEHVLGK
                     KDHSEDPNKNFKADEEPVEETPAEPEVPQVETEKVEAQLKEAEVLLAKVTDSSLKANA
                     TETLAGLRNNLTLQIMDNNSIMAEAEKLLALLKGSNPSSVSKEKIN"
     gene            1649..>2419
                     /locus_tag="VQ514_001984"
     CDS             1649..>2419
                     /locus_tag="VQ514_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET2500701.1"
                     /translation="MKINKKYLAGSVATLVLSVCAYELGLHQAQTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISEE
                     LLMKDPNYQLKDEDIISEIKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTSTNDGAVAFARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNW"
BASE COUNT          852 a          440 c          490 g          637 t
ORIGIN      
        1 gctagaggtg ttgcagtgcc acacggagat cattaccact tcatccctta ctctcaaatg
       61 tctgaattgg aagaacgaat cgctcgtatt attccccttc gttatcgttc aaaccattgg
      121 gtaccagatt caaggccaga acaaccaagt ccacaaccga ctccggaacc tagtccaggc
      181 ccgcaacctg caccaaatct taaaatagac tcaaattctt ctttggttag tcagctggta
      241 cgaaaagttg gggaaggata tgtattcgaa gaaaagggca tctctcgtta tgtctttgcg
      301 aaagatttac catctgaaac tgttaaaaat cttgaaagta agttatcaaa acaagagagt
      361 gtttcacaca ctttaactgc taaaaaagaa aatgttgctc ctcgtgacca agaattttat
      421 gataaagcat ataatctgtt aactgaggct cataaagcct tgtttgaaaa taagggtcgt
      481 aattctgatt tccaagcctt agacaaatta ttagaacgct tgaatgatga atcgactaat
      541 aaagaaaaat tggtagatga tttattggca ttcctagcac caattaccca tccagagcga
      601 cttggcaagc caaattctca aattgagtat actgaagacg aagttcgtat tgctcaatta
      661 gctgataagt atacaacgtc agatggttac atttttgatg aacatgatat aatcagtgat
      721 gaaggagatg catatgtaac gcctcatatg ggccatagtc actggattgg aaaagatagc
      781 ctttctgata aggaaaaagt tgcagctcaa gcctatacta aagaaaaagg tatcctacct
      841 ccatctccag acgcagatgt taaagcaaat ccaactggag atagtgcagc agctatttac
      901 aatcgtgtga aaggggaaaa acgaattcca ctcgttcgac ttccatatat ggttgagcat
      961 acagttgagg ttaaaaacgg taatttgatt attcctcata aggatcatta ccataatatt
     1021 aaatttgctt ggtttgatga tcacacatac aaagctccaa atggctatac cttggaagat
     1081 ttgtttgcga cgattaagta ctacgtagaa caccctgacg aacgtccaca ttctaatgat
     1141 ggatggggca atgccagtga gcatgtgtta ggcaagaaag accacagtga agatccaaat
     1201 aagaacttca aagcggatga agagccagta gaggaaacac ctgctgagcc agaagtccct
     1261 caagtagaga ctgaaaaagt agaagcccaa ctcaaagaag cagaagtttt gcttgcgaaa
     1321 gtaacggatt ctagtctgaa agccaatgca acagaaactc tagctggttt acgaaataat
     1381 ttgactcttc aaattatgga taacaatagt atcatggcag aagcagaaaa attacttgcg
     1441 ttgttaaaag gaagtaatcc ttcatctgta agtaaggaaa aaataaacta atgaaaaatg
     1501 aaagtctcga taaagaggct ttcattttta ttatgtatat atgtaaaatt cttgacaagc
     1561 aatattaaaa agagtaaact attaactagt taattaaccg gtttattact ttatagtgaa
     1621 tcaaatatac ttaagaaaag aggaaagaat gaaaatcaat aaaaaatatc tagctgggtc
     1681 agtagctaca cttgttttaa gtgtctgtgc ttatgaacta ggtttgcatc aagctcaaac
     1741 tgtaaaagaa aataatcgtg tttcctatat agatggaaaa caagcgacgc aaaaaacgga
     1801 gaatttgact cctgatgagg ttagcaagcg tgaaggaatc aatgccgaac aaatcgtcat
     1861 caagataaca gaccaaggct atgtcacttc acatggggac cactatcatt attacaatgg
     1921 taaggttcct tatgacgcta tcatcagtga agaattgctg atgaaggatc caaactacca
     1981 gctaaaggac gaggatatta tcagtgaaat caagggtggt tatgtgatta aggtagatgg
     2041 aaaatactat gtttacctta aggatgcagc tcatgcggat aatgtccgta caaaagaaga
     2101 aatcaatcgg caaaaacaag aacatagtca gcatcgtgaa ggagggactt caacaaacga
     2161 tggtgcggta gcctttgcac gttcacaagg acgctatact acagatgatg gttatatctt
     2221 taatgcttct gatatcattg aggacacggg tgatgcttat atcgttcctc acggcgacca
     2281 ttaccattac attcctaaga atgagttatc agctagcgag ttagctgctg cagaagcctt
     2341 cctatctggt cggggaaatc tgtcaaattc aagaacctat cgccgacaaa atagcgataa
     2401 cacttcaaga acaaactgg
//
LOCUS       DAVBCW010000160         2263 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.160, whole genome shotgun sequence.
ACCESSION   DAVBCW010000160 DAVBCW010000000
VERSION     DAVBCW010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.160"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            73..822
                     /gene="truA"
                     /locus_tag="VQ514_001985"
     CDS             73..822
                     /gene="truA"
                     /locus_tag="VQ514_001985"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HET2500702.1"
                     /translation="MTRYKATISYDGYAFAGFQRQPHARSVQEEIEKTLTRLNKGQAI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFTVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            812..1585
                     /locus_tag="VQ514_001986"
     CDS             812..1585
                     /locus_tag="VQ514_001986"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET2500703.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            1563..2024
                     /locus_tag="VQ514_001987"
     CDS             1563..2024
                     /locus_tag="VQ514_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2500704.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
BASE COUNT          636 a          436 c          511 g          680 t
ORIGIN      
        1 agatttttat acccatacaa tatttgtttt aggtccttgt ttacagacta atttcttgat
       61 aaaatagagg ttatgacgag atataaagca actatttcct atgatggtta tgcctttgct
      121 ggctttcagc gccagcctca tgcgcgtagc gttcaggaag aaattgaaaa aaccttgacc
      181 agattaaata aagggcaagc cattactgtt cacggtgctg gtaggacaga tagtggggtt
      241 catgccctgg gacaggtcat tcattttgac ctgccttatc agatggatga ggagaaactc
      301 cgttttgcct tggataccca gtctcctgaa gatattgatg tgatttcgat tgagcttgtg
      361 gcagatgatt ttcattgccg ttatgccaag catagcaaga cctatgagtt tactgtggat
      421 agaggacgtc ccaaaaatcc gatgcgccgt cactatgcca cccactttcc ctacccactc
      481 gatgtggaac gaatgcagat tgcaatcaaa aagctagagg gaacccatga ttttaccggt
      541 tttacagcct ctgggactag tgtagaggat aaggttcgca ccatcacaga agctagttta
      601 atagtcgatg agacaggaca atttttgacc tttacctttt caggaaatgg tttcttgtat
      661 aaacagattc gcaatatggt ggggactctt ctcaaaatcg gtaacaaccg catgccagta
      721 gagcagattg atctcatctt ggagaagaag gacaggcaac ttgcaggtcc cactgcagca
      781 ccaaatggtt tgtatttaaa ggagattcgt tatgaagaat aatcgtattt tagcactttc
      841 tggaaatgat atttttagtg gtggtggact gtcagctgat ttggctacct ataccttgaa
      901 cggtttgcat gggtttgtag cagtgacttg tttgacagcc ttgacagaaa aaggatttga
      961 agtctttcca actgatgata ccatttttca acatgaatta gatagcttgc gtgatgtgga
     1021 atttggggga attaagattg gtcttctccc tactgtcagt gtggctgaga aggccttgga
     1081 ctttatcaaa caacgcccag gagtacctgt ggtgttggat cctgtcttgg tctgcaagga
     1141 aacgcatgat gtagctgtca gtgagctctg ccaagagttg attcgctttt tcccttatgt
     1201 cagtgtgatt acgcctaatc tcccagaagc agaattatta tccggtcagg aaattaaaac
     1261 cttggaagac atgaaaactg cagcgcagaa attgcatgat ttaggagcgc cagcagtcat
     1321 tatcaaggga ggcaatcgtc ttagtcagga caaggctgtg gatgtctttt atgatggaca
     1381 gacctttact atcctagaaa atccagttat ccaaggccaa aatgctggtg caggttgtac
     1441 ctttgcctct agcattgcca gtcacttgat taaaggtgat aaacttttgc cagcagtaga
     1501 aagctctaag gctttcgttt atcgtgctat tgcacaagca gatcagtatg gagtaagaca
     1561 atatgaagca aacaaaaaca actaaaatcg cccttgtatc cctattaacc gccctttctg
     1621 tggttctagg ttatttctta aaaatcccaa cacctacagg aattctaact cttttagatg
     1681 ctggtgtctt ctttgcggcc ttttactttg gtagtcgtga aggagcggta gtcggaggac
     1741 tagcaagttt cttgattgac ctcttatcag gctaccctca gtggatgttc tttagcttgg
     1801 tcaaccatgg cttgcaggga tttttcgcag gatttaaagg aaaaagtcag tggttaggcc
     1861 ttattttagc aactattgcc atggtaggag gctacgcctt gggttctact ttgatgaatg
     1921 gctgggcagc agccctccca gaaattctac caaatttcat gcaaaatatg gtagggatga
     1981 ttgtaggatt tattcttagt caaagtatca agaagattaa gtaatactct tcgaaaatct
     2041 cttcaaacca cgtcagcttc catctgcaac ctcaaaacac tgttttgagc agcctgaggc
     2101 tagctttcta gtttgcactt tgattttcat tgagtataaa gaggttgagt aaaaagtatt
     2161 tcaaataaga aaaacgcata gtatcaggtg ttgaatgaac tgccccccaa aaagttagac
     2221 agaaaaaatc taactttttg gggtgttttt attatgaaat taa
//
LOCUS       DAVBCW010000161         2217 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.161, whole genome shotgun sequence.
ACCESSION   DAVBCW010000161 DAVBCW010000000
VERSION     DAVBCW010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.161"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            101..1078
                     /locus_tag="VQ514_001988"
     CDS             101..1078
                     /locus_tag="VQ514_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET2500705.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLAG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFEKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYKTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            1075..2157
                     /locus_tag="VQ514_001989"
     CDS             1075..2157
                     /locus_tag="VQ514_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2500706.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILTVSLASDVNVKFRKIILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
BASE COUNT          701 a          500 c          402 g          614 t
ORIGIN      
        1 aaagctaata gcttatttga aattttctcc tctctgttgg atagtcccgt ctatctatgt
       61 tataatgaaa gaaggattta aaatcggaaa aggagtattt atgctaaaat taggtgtcat
      121 cggaactggc gctatcagcc atcatttcat agaagcagcc cataccagtg gagaatacca
      181 actggtcgca atctattcta gaaaactaga aactgcagca acctttgctt ctcgctatca
      241 gaatatccaa ctattcgatc aattagaagt cttcttcaaa tcttcttttg atttagtcta
      301 tatcgccagt ccaaactcct tacattttgc tcaggcaaaa gctgccttgt ctgctggtaa
      361 acatgttatt ctcgaaaagc cagctgtcac tcaaccacaa gaatggtttg atttgattca
      421 aacagctgaa aaaaataact gttttatctt tgaagcagct cgtaattacc acgagaaagc
      481 ctttactact atcaaaaact ttttagcaga taagcaagtg ttaggagcag atttcaacta
      541 tgccaaatac tcttccaaga tgcctgactt gttggctgga caaacaccaa atgtcttttc
      601 agaccgtttt gctggtggag ccctcatgga tttggggatt tatcccctct atgctgctgt
      661 tcgtcttttt gaaaaagcta atgacgcgac ttaccatgct caacagcttg acaatagcat
      721 tgacctaaat ggagatggta tcctcttcta cccagactat caagttcaca tcaaggctgg
      781 aaaaaacatc acttccaatc ttccttgcga gatttataca acagatggaa ccttgactct
      841 caacacgatt gagcatattc gctcagctat ttttaccgac caccaaggaa atcaagtcca
      901 gcttcctatc caacaggctc ctcatacgat gactgaggaa gtcgctgcat ttgcacacat
      961 gatccagcaa ccagatctga atctctacaa aacttggctg tatgatgcag gctctgttca
     1021 tgagctacta tataccatgc gccagactgc tggcattaga tttgaggcag aaaaatgaaa
     1081 accaaactac cgactgaatg gcaagaactg agtgaccaac tcggtttcca agaattcacc
     1141 cccattcaaa ctcaactatt tgagcccctt cttgctggag aaaacctcct aggagtgagc
     1201 caaaccggaa ctggtaagac cctagcgtat ctcctaccaa gtcttctcag actacaaaag
     1261 aaaaaagccc aacaactctt gattctagca ccaaacacgg aacttgctgg acagattttt
     1321 gatgtatgta aaacgtgggc agaagctatc ggattgactg ctcaactctt cctttcaggt
     1381 tcaagtcaga aacgacaaat tgaacgtctt aaaaaggggc cagaaattct gattggaact
     1441 cctggccgta tctttgaact cattaaactt aaaaaaatca agatgatgaa tgtggaaacc
     1501 atcatcctgg atgaatttga ccaattacta gatgattctc agattcactt tgtagaaaaa
     1561 atcactcact acgcacctcg tgaccaccaa ctggtctaca tgagtgccac gaccaagttt
     1621 gaccaagaaa agattgtgcc gaatacacgc actattgatc tctctgacca aaaattggac
     1681 aatatccagc atttctacat gcaggtagac caacgtcatc gagtggatat gctacgaaaa
     1741 ctggctcatg tagaggattt ccgtggtcta gtcttcttca atagcctgtc agaccttggg
     1801 aatgccgagg aaaaactaca gtatcgtgat atattgactg tttccctcgc tagtgatgtt
     1861 aatgttaaat ttagaaaaat catcttagaa aagtttaaag ataaccaact aaccctgctt
     1921 cttgcaactg accttttggc tcgtggaatt gatatcgata gcctagaatg tgtcgtaaac
     1981 tttgatattc ctagagatag cgaaacctac acacaccgtg ctggccgtac tggccgtatg
     2041 ggcaaggaag gatatgttat tactctcgtc actcatccag aagaaatcaa aaaacttaaa
     2101 aagtttgcaa gtatccgaga aattgtccta aaaaaccaag aactctatat caaataaagc
     2161 cccattgctc tatacacatc cagtgtatgg agctttttta taagcaattc aatttta
//
LOCUS       DAVBCW010000162         2148 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.162, whole genome shotgun sequence.
ACCESSION   DAVBCW010000162 DAVBCW010000000
VERSION     DAVBCW010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.162"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            79..528
                     /locus_tag="VQ514_001990"
     CDS             79..528
                     /locus_tag="VQ514_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2500707.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGGEQVGILIINDGSQDQT
                     QEIAECLASKYPNIVRAIYQENKCHGGAVNRGLAEASGRYFKVVDSDDWVDPRAYLKI
                     LETLQELESKGQEVDAFVTNFVYEKEGQSRKKSMSYESVLPVRQIFD"
     gene            685..1095
                     /locus_tag="VQ514_001991"
     CDS             685..1095
                     /locus_tag="VQ514_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HET2500708.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKRIDQQLKVNRLLVDQLD
                     LSQVSHPKMREYLLNHIEITTVISSTLLNRSETAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            1211..>2148
                     /gene="pavB"
                     /locus_tag="VQ514_001992"
     CDS             1211..>2148
                     /gene="pavB"
                     /locus_tag="VQ514_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HET2500709.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVEKAVKDNVKDS"
BASE COUNT          660 a          410 c          477 g          601 t
ORIGIN      
        1 cggcaaggcg acgttgacgc ggtttgaaga gattttcgaa gagtataaga aaaaatcagt
       61 cccctaaagg agcagattat gaagttattg tctatcgcca tttctagcta taatgcagca
      121 gcctatcttc attactgtgt ggagtcgcta gtgattggtg gtgagcaagt tgggattttg
      181 attatcaatg acgggtctca ggatcagact caggaaatcg ctgagtgttt agctagcaag
      241 tatcctaata tcgttagagc catctatcag gaaaataaat gccatggcgg tgcggtcaat
      301 cgtggcttgg cagaggcttc tgggcgctat tttaaagtag ttgacagtga tgactgggtg
      361 gatcctcgtg cctacttgaa aattcttgaa accttgcagg aacttgagag caagggtcaa
      421 gaggtggatg cctttgtgac caattttgtc tatgaaaagg aaggtcagtc tcgtaagaag
      481 agtatgagtt acgagtcagt cttgcctgtt cggcagattt ttgactagga ccaggtcgga
      541 aatttctcca aaggccagta tatcatgata cactcgctga tttatcggac agatttgttg
      601 cgtgctagcc agttctaact gcctgaacat actttttatg tcgatgatct ctttgtcttt
      661 acgccccttc agcaggtcaa gaccatgtac tatctgcctg tcgatttcta tcgttatttg
      721 attgggcgtg aggaccagtc tgtcaatgag caagtgatga ttaagcgcat tgaccagcaa
      781 ctcaaggtca atcgactctt ggtagaccag cttgatttgt cccaagtgag tcatcctaaa
      841 atgcgagaat atctgctgaa tcatattgaa atcacgacgg tgatttccag taccctgctc
      901 aaccgatctg aaacagcgga gcatctggca aaaaaacgcc aattgtggac ctatattcag
      961 cagaaaaatc cagaagtctt tcaggctatt cgtaagacca tgttgagccg tttgaccaaa
     1021 cattctgtct tgccagatcg caaactgtcc aatgtcgtct atcaaatcac caaatctgtt
     1081 tatggattta attaatataa gtgttttata agagggattt aagaaaaatt ttaacttttt
     1141 cttagtcctt tttaatttca ggagattata ctagagtcat caaataaaga aagactctaa
     1201 ggagaatcct atgaaattca atccaaatca aagatatact cgttggtcta ttcgccgtct
     1261 cagtgtcggt gttgcctcag ttgttgtggc tagtggcttc tttgtcctag ttggtcagcc
     1321 aagttctgta cgtgccgatg tggtcaatcc gaccccaggt caagtcttac ctgaagagac
     1381 atcgggaacg aaagagggtg acttatcaga aaaaccagga gacaccgttc tcactcaagc
     1441 gaaacctgag ggcgttactg gaaatacgaa ttcacttccg acacctacag aaagaactga
     1501 agtgagcgag gaaacaaact cttctagtct ggatacactt tttgaaaaag atgaagaagc
     1561 tcaaaaaaat ccagagctaa cagatgtctt aaaagaaact gtagatacag ctgatgtgga
     1621 tgggacacaa gcaagtccag cagaaactac tcctgaacaa gtaaaaggtg gagtgaaaga
     1681 aaatacaaaa gacagcatcg atgttcctgc tgcttatctt gaaaaagctg aagggaaagg
     1741 tcctttcact gccggtgtaa accaagtaat tccttatgaa ctattcgctg gtgatggtat
     1801 gttaactcgt ctattactaa aagcttcgga taatgctcct tggtctgaca atggtactgc
     1861 taaaaatcct gctttacctc ctcttgaagg attaacaaaa gggaaatact tctatgaagt
     1921 agacttaaat ggcaatactg ttggtaaaca aggtcaagct ttaattgatc aacttcgcgc
     1981 taatggtact caaacttata aagctactgt taaagtttac ggaaataaag acggtaaagc
     2041 tgacttgact aatctagttg ctactaaaaa tgtagacatc aacatcaatg gattagttgc
     2101 taaagaaact gtcgaaaaag ctgttaaaga taacgttaaa gacagtat
//
LOCUS       DAVBCW010000163         2123 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.163, whole genome shotgun sequence.
ACCESSION   DAVBCW010000163 DAVBCW010000000
VERSION     DAVBCW010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.163"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            368..1714
                     /gene="gdhA"
                     /locus_tag="VQ514_001993"
     CDS             368..1714
                     /gene="gdhA"
                     /locus_tag="VQ514_001993"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HET2500710.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
BASE COUNT          676 a          384 c          402 g          661 t
ORIGIN      
        1 ttggatttct tctttagttg atttcatatc actattatat aatgattttt gattttagtc
       61 tggtataaat attgctttcc tccaaaatgg tcataatttt actagcaaat ctaacatatc
      121 acggataaat taacaagtga tttctgaatt gctaaacatt ttcttttctt atagcatacc
      181 atactttaag attttgtctt tgagaaagat atttccaaga aaaacgttcg tttttttatc
      241 tattttcaaa aaaaccaaaa actgaattga aagaattttt agaaaatttc tgttttttct
      301 ttacagtgaa aaaaaattct gctataatgt cttatgtaat aaaatatgac aataaaagga
      361 gaacgatatg acatctgcta aagaatatat ccaaagcgtg tttgaaactg tgaaagctcg
      421 taacgggcac gaggctgaat tcctccaagc tgttgaagaa tttttcaaca ctttagaacc
      481 tgtatttgaa aaacaccctg agtatatcga agaaaatatc ttggcacgta ttactgagcc
      541 tgagcgcgtg gtttctttcc gtgttccttg ggttgaccgt gatggaaaaa ttcaagtaaa
      601 ccgtggttac cgtgttcaat tcaactcagc tgttggacca tacaaaggtg gacttcgttt
      661 ccacccaact gtaaaccaag ggatcttgaa attcctcgga tttgaacaaa tctttaaaaa
      721 tgtcttgact ggacttccta tcggtggagg taaaggtgga tcagacttcg atcctaaagg
      781 taaaacagat gctgaagtga tgcgcttctg ccaaagcttc atgactgaat tgcaaaaaca
      841 catcggacca tcacttgacg tacctgctgg tgatatcggt gttggtggac gtgaaattgg
      901 ttacctttac ggtcaataca aacgtcttaa ccaatttgat gctggtgtct tgactggtaa
      961 acctcttgga tttggtggta gcttgattcg tccagaagca actggttacg gtttggttta
     1021 ctatactgaa gaaatgctca aagctaacgg taacagcttt gctggtaaga aagtcgttat
     1081 ttcaggttct ggtaacgttg ctcaatatgc tcttcaaaaa gcaactgaac tcggtgcaac
     1141 tgttatttct gtttctgact caaatggtta tgtcatcgat gaaaatggta tcgacttcga
     1201 tcttttggtt gatgttaaag aaaaacgtcg tgctcgtttg actgagtatg cagctgaaaa
     1261 agcaactgca acctaccacg aaggtactgt atggacttac gctggaaact atgacattgc
     1321 tcttccatgt gccactcaaa acgaaatcaa cggtgaagca gctaaacgtt tggttgctca
     1381 aggcgttatc tgtgtatctg aaggtgccaa catgccgagc gaccttgatg ccatcaaagt
     1441 ttacaaagaa aatggtatct tctacggacc tgcaaaagct gccaacgctg gtggtgtagc
     1501 cgtttcagct cttgaaatga gccaaaacag tcttcgcctc tcatggactc gtgaagaagt
     1561 tgatggccgt ctcaaagaca tcatgacaaa catcttcaac acagctaaaa cgacttcaga
     1621 aacatacggt cttgataaag actaccttgc aggagctaac attgctgcct ttgaaaatgt
     1681 agcaaatgcc atgattgcac aaggtattgt ttaagattta tttactcaat cctcaatcac
     1741 tacgattggg gatttttagc ttgaattaaa aaattggaaa atctaattaa ataaaatgaa
     1801 atgaaatgaa ataaaaactg aacaaattga ttgcggaatt caaaccaatt tctaacatac
     1861 ttctagaaat taatttgact tttctaatcg atttgttcat atcttatttc aatttactat
     1921 acttacttaa ccctttttat actcaatgaa aatcaaagag caaactagga agcgagccgc
     1981 aggctgtact tgagtacggc aaggcaaagc tgacgtggtt tgaagagatt ttcaaagagt
     2041 attaaacaaa aagaaaaacc gctactccta agaatagcga tttaatcatg tttttaagct
     2101 actactgtag ttgctctatt att
//
LOCUS       DAVBCW010000164         2112 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.164, whole genome shotgun sequence.
ACCESSION   DAVBCW010000164 DAVBCW010000000
VERSION     DAVBCW010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.164"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            99..1592
                     /locus_tag="VQ514_001994"
     CDS             99..1592
                     /locus_tag="VQ514_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HET2500711.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            1605..>2112
                     /locus_tag="VQ514_001995"
     CDS             1605..>2112
                     /locus_tag="VQ514_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2500712.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLREHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKRLITKRK
                     LQLDELNLL"
BASE COUNT          745 a          321 c          417 g          629 t
ORIGIN      
        1 attcaggtga cgcatagata tagttaattg aagctttgtt tgaaatctga taaaataatg
       61 atattactaa gttttaaaaa ctaaagaaaa gggaagatat gattacaggc gaattaaaaa
      121 ataaaatcga tcagctgtgg gaaattcttt ggacagaagg aaacgcaaat cctttaacaa
      181 atattgaaca gttgacttat ctcttattta tgaaagattt ggatagtgtc gagcttggac
      241 gtgaaagtga tgctgaattt ctagggattc cttatgaggg agtttttcca aaagataaac
      301 ctgaataccg ttggtcaact tttaaaaata taggagatgc tcaggaagtt tatcgtttaa
      361 tgactcagga gatttttccg tttattaaaa atctcaaggg ggatacagat gatacagcct
      421 tttcacgata tatgcgagaa gctatttttc aaataaataa acctgctacg cttcaaaagg
      481 caatttctat cttagatgtt tttccaacta ggggattaga tgtagatttt gataatgaca
      541 aacaaagtat tactgatatc ggagatatct atgaatatct gttatcaaaa ttgtcgaccg
      601 caggtaaaaa tggacagttc cgtacacctc gtcacatcat cgatatgatg gttgagttga
      661 tgcaaccgac tatcaaagat atcatctcag atcccgctat gggttctgct ggcttcttag
      721 tatctgctag ccgttactta aagcgtaaga aagatgaatg ggaaaccaat acagataata
      781 tcaatcattt tcataatcag atgtttcatg gaaatgatac ggatacgact atgttgagac
      841 ttggggcgat gaacatgatg ctacatggag tagaaaatcc acaaatcagt taccttgact
      901 cgctgtctca agataatgaa gaagccgata aatatacttt ggttttagca aatcctcctt
      961 ttaagggctc acttgactac aattcaacct ctaatgacct tcttgcaacc gtaaaaacca
     1021 aaaaaacaga attactcttt ctttctcttt tcttgcgaac tttaaaacca ggtggacgag
     1081 cagcagttat cgtacctgat ggtgtccttt ttggttcgtc taaagctcat aaaggaattc
     1141 gtcaggaaat tgtagagaat cataagcttg atgctgtaat ctcaatgcct agtggtgtgt
     1201 tcaagcctta tgctggagtt tcaactgcca ttctcatctt tacaaaaact ggtaatggtg
     1261 gtactgacaa agtctggttt tacgatatga aagcggatgg tttaagtttg gatgataagc
     1321 gacaaccgat tagcgacaat gatattccag atattatcga acgctttcat catcttgaaa
     1381 aagaagcaga acgtcagaga acggatcaat ctttctttgt tccagttgct gagataaagg
     1441 aaaatgatta tgatttgtct atcaataaat ataaagagat tgagtatgaa aaagttgagt
     1501 atgaaccaac agaggtcatc ttaaagaaaa tcaatgattt agaaaaagaa attcaagctg
     1561 gcttggctga attagaaaaa ttactcaagt agagaggtgg ctgtatgaaa aaagtgaagt
     1621 tgggggaagt cttatctcta aaaaaaggca agaaagccac tgtacttgct gaacaaacaa
     1681 ctctaagcca acgttatatt caaatagatg atttaagaaa taataataat ttaaaattca
     1741 ctgaaagttt aaatatgact gaagcactcc cagatgatat tctgatagca tgggatggag
     1801 ctaatgcagg aacagttggt tatggattat cgggagctgt tggtagtaca attacggtct
     1861 taaaaaagaa tgagcgatac aaagaaaaaa ttatatcaga ttacttggga gtctttttgg
     1921 aaagtaaatc gcagtattta cgagaacatt caacaggtgc aacaattcct catttaaaca
     1981 agaatatatt acttgattta caattagaat tgctaggtat cgaagaacaa gagaacatta
     2041 tctgtattct taatacgatt aaaaggctta ttactaaaag aaaattgcag ttagatgaac
     2101 taaacttgct cg
//
LOCUS       DAVBCW010000165         2089 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.165, whole genome shotgun sequence.
ACCESSION   DAVBCW010000165 DAVBCW010000000
VERSION     DAVBCW010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.165"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(88..292)
                     /locus_tag="VQ514_001996"
                     /pseudo
     CDS             complement(88..292)
                     /locus_tag="VQ514_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            649..>2089
                     /gene="pcpA"
                     /locus_tag="VQ514_001997"
     CDS             649..>2089
                     /gene="pcpA"
                     /locus_tag="VQ514_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HET2500713.1"
                     /translation="MKKTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSII
                     QQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVA
                     SQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDGA
                     FDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLT
                     LPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESY
                     KTPKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERL
                     AFYGNLELKELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEI
                     HIKNKSTEFSVKKDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETD
                     VAKPKKNSNQGVVGWVKDKGLWYYLNESGS"
BASE COUNT          754 a          293 c          336 g          706 t
ORIGIN      
        1 gtgtttaagt tgataagctg tttttaacac agctaacatc tcttcaaaag tggtgcgctg
       61 aacaccaaca agacgcttaa atcgtgcatc ggttagttgt ttacttgctt cataatttcg
      121 cagggagtct attgactctt tggtaggtgt caatgttttt ttcatctatc ccgagaatta
      181 ttttcccgcc atttgtattt gcaaatgctg agtaggtttc ccagaaagac tctggaagat
      241 tgtttttagc ttttttgtat tctaaatcaa ccccttcaaa ttttaagtcc atatttttcc
      301 tttacatctg ttttttgtgg ttctggtatt tgttcaagtt gagtgataat atagcgaatt
      361 gaatttcgag agtttttact cagttaattt cttttttaac ccactttaat ttctttttta
      421 acccacttta atttcttttt taacacgggt taaaaaagaa attaaagtgg gttaattttt
      481 cttgacttaa aatttaaaaa tgatatactg tgtttatgga gagatgacaa tttataaaat
      541 gtactactct attctactag ataatgattg aataaaactt tttataacaa cggctagcaa
      601 agttataatt ttatatctat tagaaaataa atatgtaagg aaatttaaat gaaaaaaact
      661 acaatattat cattaactac agctgcggtt attttagcag catatgtccc taatgaacca
      721 atcctagcag atactcctag ttcggaagta atcaaagaga ctaaagttgg aagtattatt
      781 caacaaaata atatcaaata taaggttcta actgtagaag gtaacatagg aactgttcaa
      841 gtgggtaatg gagttactcc tgtagagttt gaagctggtc aagatggaaa accattcacg
      901 attcctacaa aaatcacagt aggtgataaa gtatttaccg ttactgaagt agctagtcaa
      961 gcttttagtt attatccaga tgaaacaggt agaattgtct actatcctag ctctattact
     1021 atcccatcaa gcataaaaaa aatacaaaaa aaaggcttcc atggaagtaa agctaaaact
     1081 attatttttg acaaaggcag tcagctggag aaaattgaag atggagcttt tgatttttct
     1141 gaattagaag agattgaatt gcctgcatct ctagaatata ttggaacaag tgcattttct
     1201 tttagtcaaa aattgaaaaa gctaaccttt tcctcaagtt caaaattaga attaatatca
     1261 catgaggctt ttgctaattt atcaaattta gagaaactaa cattaccaaa atcggttaaa
     1321 acattaggaa gtaatctatt tagactcact actagcttaa aacatgttga tgttgaagaa
     1381 ggaaatgaat cgtttgcctc agttgatggt gttttgtttt caaaagataa aacccaatta
     1441 atttattatc caagtcaaaa aaatgacgaa agttataaaa cgcctaagga gacaaaagaa
     1501 cttgcatcat attcgtttaa taaaaattct tacttgaaaa aactcgaatt gaatgaaggt
     1561 ttagaaaaaa tcggtacttt tgcatttgca gatgcgatta aacttgaaga aattagctta
     1621 ccaaatagtt tagaaactat tgaacgttta gccttttacg gtaatttaga attaaaagaa
     1681 cttatattac cagataatgt taaaaatttt ggtaaacacg ttatgaacgg tttaccaaaa
     1741 ttaaaaagtt taacaattgg taataatatc aactcattgc cgtccttctt cctaagtggc
     1801 gtcttagatt cattaaagga aattcatatt aagaataaaa gtacagagtt ttctgtgaaa
     1861 aaagatacat ttgcaattcc tgaaactgtt aagttctatg taacatcaga acatataaaa
     1921 gatgttctta aatcaaattt atctactagt aatgatatca ttgttgaaaa agtagataat
     1981 ataaaacaag aaactgatgt agctaaacct aaaaagaatt ctaatcaggg agtagttggt
     2041 tgggttaaag acaaaggttt atggtattac ttaaatgaat caggttcaa
//
LOCUS       DAVBCW010000166         1860 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.166, whole genome shotgun sequence.
ACCESSION   DAVBCW010000166 DAVBCW010000000
VERSION     DAVBCW010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.166"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(3..968)
                     /locus_tag="VQ514_001998"
     CDS             complement(3..968)
                     /locus_tag="VQ514_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET2500714.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKVQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKFIS"
     gene            complement(1086..1499)
                     /locus_tag="VQ514_001999"
     CDS             complement(1086..1499)
                     /locus_tag="VQ514_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HET2500715.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSSSALNMIVNLPEQ
                     FVFGKPLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWISEHSVREKVKK"
     gene            complement(1496..1732)
                     /locus_tag="VQ514_002000"
     CDS             complement(1496..1732)
                     /locus_tag="VQ514_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HET2500716.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
BASE COUNT          544 a          362 c          274 g          680 t
ORIGIN      
        1 ttttaagaga tgaatttttg gtgtgaatgc ttgacattat tttgattgac catggcatag
       61 gccagggttg tgtcaatctt gctatgacct agcagttttt ggacttgttc gataggcata
      121 cctttatcaa ttgccttagt cgctaaagtt cttctgaact tatgtgggtg aaccttttgt
      181 atgccgagtt tgtctcctaa ctttcttaag cgaatctcta caccagcaat tccaagcctc
      241 tggacttttc caactagcgt tacaaaaaga gcagggtgac tatcttttct gtcgttaaga
      301 taatttctta aatgaatttt cgtacgagcg tcaaaatata ctggtctctc cttctttcct
      361 ttaccaaaga caacacactc tctgttttca aaatcaatat ctgaacgatt caactgtaca
      421 agctccccta cacgcatacc tgtcgatgct agtaggtcta ttattgccaa atctctcaaa
      481 ttttcacagt tatcacgcat aatttccaaa tgttcatcag tataagtttc cttcacattt
      541 tgctcagtct taattttctt tatccgtcga atgggatttt taatgatata ttcctcttgc
      601 tcaagccaag caaaaaaaga agacaatata cgcctaatat tgtctaaatt tgccttacta
      661 caatcctttt cagactggta atttgctaaa taactcctta aatcatctgt tgtgagttgt
      721 gtcacagact ctttaatagc attaaacaag ttttcaatcg tcctctgata ataacgaatt
      781 gtttttgagc tacagccctc tacctgcttg gcggtaataa atagttgcaa caggttatta
      841 ttcgaatact tctgctgctt ttttccgtct actagatgat gttctaaact ctctctcaac
      901 gctagtaatt gttcattatt taaaagaggt aacatttctt gcatgataat tgtaactttt
      961 gattccataa tttctcactt tcttattcat caataaaaag agacaatatc agtttctgca
     1021 ttacagaatt ctaatattgt ctgttcattt attttcttgt gctcattcaa atccagcata
     1081 ctaggttact ttttgacctt ttctctaaca gaatgttcag aaatccattt ggagtagctt
     1141 gtcatttttt catcagttaa agcttctact gcgatggtta cacacatttt tactgcttct
     1201 tctaccgtta cagaaaaacg atagccgttt aattgtaaaa atttgaccaa aacgaagaaa
     1261 gcagttcttt tattagcatt agcaaaaaca tgcttcttta tcaattggac aaatagtatc
     1321 gttgctttat caaaaattgt tggataaaga ggcttcccaa agacaaattg ttctggtaag
     1381 ttcacaatca tatttaaggc agaagaacta actgtttgaa ttttctcatt tggagaatac
     1441 cgttgaattg ctaaagcatt tattttttca atttgctttt ctgtcaaata gattgtcatt
     1501 tttccaccaa agctttgaaa acatcgtcgt attcgtcaaa tattttatct aaatttaagt
     1561 caaaggcatc atcagataca taagaaacct gctgccccaa ttcttcagga gtaaagataa
     1621 tctcaccact tggtaacaat ttcgcttcgt atttaacacc acttggaata ttaaattcac
     1681 ttggaatcgt aatggtgatt gaatttcctt gttttcttgt ttttactacc atctgatttc
     1741 ctcctttgaa gtattataac aaatcattac cgtaattaca atggcagaat atcagccaaa
     1801 atactcctgc atcagagatt tcttcaaagt ttcaagttct tccagagatt tttggattgc
//
LOCUS       DAVBCW010000167         1795 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.167, whole genome shotgun sequence.
ACCESSION   DAVBCW010000167 DAVBCW010000000
VERSION     DAVBCW010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.167"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            <1..944
                     /locus_tag="VQ514_002001"
     CDS             <1..944
                     /locus_tag="VQ514_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HET2500717.1"
                     /translation="PSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFL
                     GSNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDS
                     KKNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYM
                     LIPNVDRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVL
                     KRGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKN
                     HSDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            1324..1709
                     /gene="tnpA"
                     /locus_tag="VQ514_002002"
                     /pseudo
     CDS             1324..1709
                     /gene="tnpA"
                     /locus_tag="VQ514_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          635 a          309 c          356 g          495 t
ORIGIN      
        1 taccgtctgc tttcgttgga acgagcaaac tagatgatgc caatgcgaca gcaaccatgg
       61 atttcaccaa ctggaatcaa acattgactg ctcataagag ctggtttatg ctaaaggata
      121 agattgcctt tttaggaagc aatatccaaa acacttcaac agatactgct gcaactacaa
      181 ttgaccagag aaaactggaa tcaagtaatc catataaagt ctatgtcaat gataaagaag
      241 cctcccttac agaacaagaa aaggattatc ctgaaaccca aagtgtcttt ttagaatcgt
      301 ccgattcgaa aaagaatatt ggttactttt tctttaagaa gagttcaatc agtatgagta
      361 aggctttgca aaagggagcc tggaaggata tcaatgaagg acagtcagac aaggaagttg
      421 aaaatgaatt tcttactatt agtcaggctc ataagcaaaa tggagattct tatggctata
      481 tgctcattcc taacgtggat cgtgccacct tcaatcaaat gataaaagag ttagaaagta
      541 gcctcatcga aaataacgaa acccttcagt ctgtttatga tgctaaacaa ggagtttggg
      601 gcattgtgaa atatgatgat tctgtctcta ctatttccaa ccaattccaa gttttgaaac
      661 gtggagtcta taccattcga aaagaagggg atgaatataa gattgcctac tataatcctg
      721 aaacccagga atcagctcca gatcaggaag tctttaaaaa gctagagcaa gcagctcagc
      781 cacaagtaca gaattcaaaa gaaaaggaaa aatctgaaga agaaaagaac cattcggatc
      841 aaaagaatct ccctcagaca ggagaaggtc agtcaatctt ggcaagtcta gggttcttgc
      901 tacttggggc gttttattta ttccgtagag gaaagaacaa ctaatttgtt catcatctag
      961 atgaattaca tgaaactgtg gaaaatagaa aaaatccgaa gaaggccaaa tatcttcgga
     1021 tttttctttt aaaaagggat aagttgatac ttatcctttt ttacttcatt atgtgactat
     1081 agcgttttga aactagaata ggacgtcata actgctaaaa gatttctata aattcatttg
     1141 attttcctaa tcaatttgtt cgtatcctat ttcactccac tatattagtc cgtctcgctc
     1201 cgtgaggtgc gagacaaaac aaccacccgc tatgcgggtg cgcgtcgaag gctataccaa
     1261 aaaactccaa acgcgataca ataaaggtgt tcaatccaat tgtaaagcga aaggagaaaa
     1321 aatatggcac aaaagactca tagtttatca tacacaaagt ggatgtgttc tatcatattg
     1381 tgttcacccc taagtataga cgaaaagtca tctataatca atatagaagt agtttaggcg
     1441 aaatatttca tcgcttgtgt ggttataaag gtgttgaaat gatcgagggt cacttaatgc
     1501 cagaccatgt acacatgtta gtcagtattc cactaaggct aagtgtttcg agtttcatgg
     1561 ggtatttaaa aggcaaaagt gcactcatga tgtttgacaa acacgccaat ctcaagtaca
     1621 agtttgggaa tcggcatttc tgggcggaag gttattatgt aagtacagta gggcttaata
     1681 aagccacact taagaaatat agtcaagatt aaagaaatta tcgagtggat gatttcttca
     1741 cgagtatgaa aatttgagaa cgagtaaagt atgatatata gtaaaatgaa ataag
//
LOCUS       DAVBCW010000168         1690 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.168, whole genome shotgun sequence.
ACCESSION   DAVBCW010000168 DAVBCW010000000
VERSION     DAVBCW010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.168"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(242..1006)
                     /locus_tag="VQ514_002003"
     CDS             complement(242..1006)
                     /locus_tag="VQ514_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HET2500718.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLDEEI
                     DRYAREVGADCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPSRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            1076..1237
                     /locus_tag="VQ514_002004"
     CDS             1076..1237
                     /locus_tag="VQ514_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
                     /protein_id="HET2500719.1"
                     /translation="MDKKQAYIVSCHSGLRSYIAEPILKQAGFTAQNLDGAYSLYKMV
                     NPEGVEYGN"
     gene            1227..1523
                     /locus_tag="VQ514_002005"
     CDS             1227..1523
                     /locus_tag="VQ514_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET2500720.1"
                     /translation="METSISMADFYGKYQNENLELIDVREAHEFQAGHAPGAKNLPLS
                     TLEQGYKELKPDHEYYVICQGGVRSASTCQFLISQGLTVTNVEGGMNAWPGQVE"
BASE COUNT          529 a          413 c          329 g          419 t
ORIGIN      
        1 gaagctgacc tggtttgaaa agattttcga agagtatcat ttcatctcac tctaaatctt
       61 accaacataa aacgcatagt atcaaggttt tcatcacctg atatgatacg tttttctgat
      121 tttaaagatt tttcccaacc tcaacatgct taggacaaga ggaaggtgac ctcgtttaaa
      181 catatttttg aaaagtatga gctagacatt ttatgataaa acaaatcctt tgccagtaca
      241 actaaaggtg gtgaacactt gctaaactca gttctagcgc cttattaaaa gcttccgagc
      301 cccagtcacg actgtcgtac tggtccaagt ccgctagaga atctgctgtg aacaacaatt
      361 caccccagag aaccccacgc aattgagcta ctgccgcaag agcagaacac tccatctcca
      421 caacagcaca gccttcttcc ttacgataag ccaccttttc agccgtttct cggtaaaaac
      481 cgtctgtcgt ccaggtcatg acttcttcat aaggaatccc tctgtcttcc aaaacttcct
      541 caatagcagc aatagcctct ggctgcattt ccatataacg agaaggtgcc acatagtggt
      601 aactggctcc ttcatctcgc agagcgcgaa cagggactag aaaggcattt tcctctatat
      661 cagctagaac accacaggtc ccagtagaga taatctgctc cacaccatag ccaatcaacc
      721 aatccataaa ctgggctgct ggagcggagc caacaggagc ctgagccaga cagacctcct
      781 cacccttgta gttgatgaca tagactggat aggtcttggt agcagaaacg aattcgccga
      841 cacaatccgc ccctacttcc cttgcatagc ggtcaatctc ctcatctaaa aatgcataaa
      901 cacacttctt tggcaactgc aagtccagcc cctcgtgatt gggcatgata accgcctgag
      961 gattgtcatc aaactctaaa ataggaatcg catgtttctg aatcatagcc cacctcctct
     1021 aattttgtac tattgtatca aaaactgaca aagtgtcaag gaggaagctg atgaactgga
     1081 caaaaaacaa gcctacatcg tcagctgcca cagtggtttg cgcagctata tcgcagagcc
     1141 tatcctcaag caagcaggat ttaccgccca aaaccttgac ggcgcttatt cactatacaa
     1201 aatggttaac ccagaaggag tagaatatgg aaactagtat cagcatggct gacttttatg
     1261 gaaaatacca aaatgaaaat ctagaactta tcgatgtccg tgaagcacat gaattccaag
     1321 caggacatgc accaggtgcc aaaaatcttc cattaagtac cttagagcaa ggctacaaag
     1381 aactcaaacc tgaccatgaa tactatgtta tttgtcaagg aggagtccgc tctgcatcta
     1441 cctgccaatt tctcatctcc caaggcctca ccgttaccaa cgtagaaggt ggtatgaatg
     1501 cttggcctgg tcaagtagaa taatcaagag gaactgggca aaaactcaat ttcatgagaa
     1561 gatgaagtaa tactcaatga aaattaaagt gcaaactagg aagttagccg caggctgctc
     1621 aaaacactgt tttaaggttg tggatggaac tgacgaagtc agctcaaaac actgttttga
     1681 ggttgtggat
//
LOCUS       DAVBCW010000169         1639 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.169, whole genome shotgun sequence.
ACCESSION   DAVBCW010000169 DAVBCW010000000
VERSION     DAVBCW010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.169"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            109..408
                     /locus_tag="VQ514_002006"
     CDS             109..408
                     /locus_tag="VQ514_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic biosynthesis protein"
                     /protein_id="HET2500721.1"
                     /translation="MDSYAINFWESKGVGIDKKILEFIQKNITVIKNKNFLNKDFDIY
                     VKKIHNLFFVISTLVSKEQYMGIVSSQIHMFANRMGISPTIEVTMLDFFLREDSD"
     gene            410..1180
                     /locus_tag="VQ514_002007"
     CDS             410..1180
                     /locus_tag="VQ514_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HET2500722.1"
                     /translation="MEKYFFDISIFEKIKKVLSYQSKYWEISKPRDKYSFKSQNRISG
                     SNSIKFKDFELDYELESLGKILLKRRSFLLNEFDKNASFDKLIYLLKTVFSPTNCKFV
                     FGEEVPLFSYPTSGALNSITPFVYIDNMDEVKNGIYEVKYDGLEFKKSMNIIDLETFT
                     PITSKNSDVQNNSFKKVNMIIMLCSNYENIFNKYGLLAYRLLHLEAGHIAQNIQLVST
                     ALGIKSLPMGGWFDDKIETFINEISDKKNIYLYAIAVG"
     gene            1200..1364
                     /locus_tag="VQ514_002008"
     CDS             1200..1364
                     /locus_tag="VQ514_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500723.1"
                     /translation="MNAEDKFRELENENKLLKLHNKELENEIQNGWKFWIWVTVPILG
                     LIYAIIQKIF"
BASE COUNT          601 a          180 c          268 g          590 t
ORIGIN      
        1 gataactatt attacgaaaa aaaattcttg tgtaaaattc tattgacttt ctatattatt
       61 tttgtatttc ataacgaatg taatttaaca agtgaagaaa ttttaaagat ggattcctat
      121 gctattaatt tctgggagag taaaggggtc ggtattgaca aaaaaatctt ggaatttatt
      181 caaaaaaata ttacagttat taaaaataaa aattttttga ataaagattt tgatatttat
      241 gttaaaaaga ttcataattt attctttgtc atttctactt tagtttcaaa agagcaatac
      301 atggggatag tttcttccca aatacatatg ttcgcaaata ggatgggaat ttctcccacc
      361 attgaagtta ccatgctaga tttttttctg agggaggatt ctgattaata tggaaaaata
      421 tttttttgat atttctattt ttgaaaaaat aaaaaaagta ttatcatacc aatctaaata
      481 ttgggaaata tcgaagccta gggataagta cagtttcaaa agtcaaaata gaatatctgg
      541 ctctaatagc attaaattta aagattttga gttagattat gaactggagt cacttggtaa
      601 aatactttta aaaaggagat cgtttttact aaatgaattt gataagaatg cgagttttga
      661 caaactgatt tacttgttga aaactgtatt ctctcctaca aattgcaagt ttgtatttgg
      721 agaagaggtt cctttattta gctatcctac atcaggtgcg ttaaattcaa ttactccttt
      781 tgtttatatt gataatatgg atgaagtaaa gaatggtata tatgaagtaa agtatgacgg
      841 acttgaattt aaaaaaagta tgaatattat agacttagaa acatttaccc caataacaag
      901 taaaaacagt gacgtacaaa ataattcatt taaaaaggta aatatgataa ttatgttatg
      961 ttcgaattat gaaaatattt tcaataaata tggattatta gcttatcgac ttttgcattt
     1021 agaggctggt catattgcac aaaatatcca gcttgtttct actgcgctag gaataaaaag
     1081 tttaccgatg ggaggttggt ttgatgataa aatagaaact tttattaatg aaattagtga
     1141 taaaaaaaat atttatctct atgcaattgc agttggataa atttaaggag gaagtgtaga
     1201 tgaacgcaga ggataaattt cgcgaactag aaaatgaaaa taaacttcta aaacttcata
     1261 acaaggagct tgagaatgaa attcaaaatg gatggaaatt ttggatatgg gtaactgtcc
     1321 ctattttagg tttgatatat gctattattc aaaaaatttt ttaattatgc tagtttgtca
     1381 ggttgagtag tttttgttca aaatttaata aacttttttc aatgaagatt atctaaagat
     1441 tgcttctcag atgagaatgg cttctctatc ttatataaat tatcttcata gagtgtcctg
     1501 ttattcatgt tatcacagaa tagatagttt ttttggctct ataatatttg tagtgggtaa
     1561 atcccctata gatattatgg agcctatttt tgtgtagaaa aaaagtccca tatcatctat
     1621 aatgaaaagc aaccaaact
//
LOCUS       DAVBCW010000170         1596 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.170, whole genome shotgun sequence.
ACCESSION   DAVBCW010000170 DAVBCW010000000
VERSION     DAVBCW010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.170"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(6..605)
                     /locus_tag="VQ514_002009"
     CDS             complement(6..605)
                     /locus_tag="VQ514_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HET2500724.1"
                     /translation="MTFCAGYVDAYTFIVRGNTLVAGQAGNVVFLSVELIQNNVSDVR
                     DKLLTLITFIMGVFLLTIYKEKLRIVKKPILSLIPLAILSIIIGFVPQTVDNIYLVQP
                     LAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLAFGDYFRTKHTPFLREGFIFVSLL
                     SSFVLGDVFSAYLTIFYHEKTILGVPIMMSIFYLSMLFA"
     gene            1075..1374
                     /locus_tag="VQ514_002010"
     CDS             1075..1374
                     /locus_tag="VQ514_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HET2500725.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
BASE COUNT          603 a          304 c          251 g          438 t
ORIGIN      
        1 tgccactagg caaaaagcat actgagataa aaaatgctca tcataatagg aacaccaaga
       61 atggtctttt catgatagaa aatcgtcaaa taggctgaaa agacatcgcc aaggacaaaa
      121 ctactaagca ggctaacaaa tatgaaccct tcacgcaaaa aaggagtgtg cttagttcgg
      181 aaataatctc caaaagctag cattgttcgt ttgatattcc ctgtcataaa ggcattattg
      241 taggcaatac ccgaaacctc accaaaagca gttgtcacca gtcccataca gaaggccaag
      301 ggctgcacta gatagatatt atctacagtt tgtggcacaa aaccaataat tattgatagg
      361 attgctaaag gaatcaagga cagaataggt tttttcacaa ttctcaattt ttccttataa
      421 atcgttaata aaaagactcc cattataaac gttattaagg ttaaaagctt atccctaaca
      481 tccgaaacat tattttgtat tagctctacc gaaagaaaga caacatttcc agcttgtcca
      541 gctacaaggg tattccctcg aacaataaaa gtgtaagcat ccacatatcc agcacaaaat
      601 gtcaaaaaag tgctaacctt ttagactgac gtgatatttt tcttataggt aataacctca
      661 ttttccctcc cattgtattt tctcttagaa atattgtacc atttctttct aaaaaatcgt
      721 agactaccat ttagatttta ctattagcat aaaaataata atagacaact atttatccaa
      781 aaatagatag aggtaacatg tttgcaaaca aagcatacga acctttagta aaatcatttc
      841 catgaaacta gaatagagcc cctcttagca aaaatcatta ttttaattta tttctaatca
      901 ctccttgaca taaataactc tcaccaataa aagactatgt cttaaaaaaa tggtataata
      961 aaatcaatac ctgggcttga tggctatgct actaataaca attaggagag aaaatcaggc
     1021 acttgttaac aacaaggatt atccccttga gatgaaagga actttagaaa tcttatgatg
     1081 aacatgcaaa acatgatgcg tcaagcacaa aaacttcaaa aacaaatgga acaaagccaa
     1141 gctgaacttg ctgctatgca atttgttggc aaatctgctc aagatcttgt ccaagcgacc
     1201 ttaactggcg ataagaaagt tgtcagcatt gatttcaatc cagctgtcgt tgacccagag
     1261 gaccttgaga ctctttctga tatgaccgtt caagccatca actctgctct tgaacaaatc
     1321 gatgaaacta ctaagaaaaa actgggtgct ttcgctggga aattgccatt ctaaaaacaa
     1381 ggagctagaa caatacttgt cgataacaaa ggctaagaaa ggtgcaaaaa ttataaaaaa
     1441 cgcatagtat caagcaacct aaaactttgg gctctataat atttgtagtg ggtaaatcca
     1501 ctatagatat tatggagcct attttattgt agaaaaaaat cccataagat ctataatgaa
     1561 aagcgaccaa acaactcatt agaaagattc atatgg
//
LOCUS       DAVBCW010000171         1594 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.171, whole genome shotgun sequence.
ACCESSION   DAVBCW010000171 DAVBCW010000000
VERSION     DAVBCW010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.171"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            100..1575
                     /locus_tag="VQ514_002011"
     CDS             100..1575
                     /locus_tag="VQ514_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HET2500726.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNIVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLQGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
BASE COUNT          441 a          322 c          319 g          512 t
ORIGIN      
        1 tccagtattt gacaatattg aagcagctat taaagaacac ggattaagct aattctaatt
       61 tgttaacatt catataacat aaaaaaggga ggaactgtta tggatgcaat ctttgaccta
      121 atcggaaagg ttttcaatcc cattttagaa atgggtggac ctgtcatcat gttaatcatt
      181 ttgacagtat tggctttact ttttggagtg aaattctcca aagcgcttga aggtggtatc
      241 aaacttgcca tcgctcttac aggtatcggt gctatcatcg gtatgctaaa tggtgctttc
      301 tcagcatcac ttgcaaaatt cgttgaaaac actggtatcc aattgagtat taccgacgtt
      361 ggttgggcac cactcgcaac aatcacttgg ggttctgctt ggacactata cttcttgctc
      421 atcatgttga ttgtcaacat agtgatgcta gctatgaaga aaacagatac acttgacgtt
      481 gatatctttg atatctggca cttgtctatc acaggtctct tgattaaatg gtatgctgat
      541 aacaatggtg tgagtcaagg ggtttcactc tttattgcta cagcagctat cgtccttgtc
      601 ggtgtgttga aaattatcaa ctctgacttg atgaaaccta catttgatga ccttcttaac
      661 gccccaagtt catcaccaat gacatcaact cacatgaact acatgatgaa cccagttatc
      721 atggttttgg ataagatttt tgaaaaattc ttcccaggcc ttgataaata tgactttgat
      781 gctgctaaat tgaacaagaa aatcggtttc tggggatcta aattcttcat cggtttcatc
      841 cttggtatcg ttatcggtat tatgggaact ccacatccaa ttgcaggtgt tgcagatgca
      901 gataaatggc gtcttgttat caaaggatgg ttgtctcttg gtttgactgc cggtgtatct
      961 ttggaactct tctcacttat cggttcatgg ttcatcgcag ccgtagaacc actatcacaa
     1021 ggtattacaa acgttgctac taaacgtctt caaggacgta aattcaatat cggtcttgac
     1081 tggccattca tcgctggtcg tgctgaaatc tgggcttgtg ccaacgtact tgcaccaatc
     1141 atgttgattg aagcagtgct tctttcaaaa gttggaaatg gtatcttgcc acttgcaggt
     1201 atcatcgcta tgggtgttac tccagctctc ttggttgtaa ctcgtggtaa attgctccgt
     1261 atgattatct tcggaacact cttgttgcca ctcttccttc tttcaggtac acttattgca
     1321 ccatttgcaa cagaacttgc taaaggtgta ggtgccttcc cagaaggtgt gagccaaact
     1381 caattgatta ctcactctac tcttgaagga ccaatcgaaa aacttcttgg ttggacaatt
     1441 ggtaacacta caactggtga tatcaaagca atccttggtg cagtagtctt ccttgtattc
     1501 tatatcggta tctttgcttg gtacagaaaa caaatgatca aacgtaacga agagtacgca
     1561 gcaaaagcaa aataatgcgc tcctataaaa tata
//
LOCUS       DAVBCW010000172         1581 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.172, whole genome shotgun sequence.
ACCESSION   DAVBCW010000172 DAVBCW010000000
VERSION     DAVBCW010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.172"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(120..1316)
                     /gene="tuf"
                     /locus_tag="VQ514_002012"
     CDS             complement(120..1316)
                     /gene="tuf"
                     /locus_tag="VQ514_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET2500727.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
BASE COUNT          441 a          311 c          328 g          501 t
ORIGIN      
        1 tatagtttgc tctttgattt tcattgagta ttagttcaat agctaaaaaa agaaccttgc
       61 caagcaagat tcttttagtg tctgacttaa ataattgttc ttctgggaac taaatcgaat
      121 taagcttcga tttctgtaac catacctgaa ccaacagtac gtccaccctc acggatagag
      181 aatgtagtac cttgttctac ggcgattggg tgaatcaact caacatcgat tgtcacgtta
      241 tcaccaggca ttaccatttc agtacctgct ggaagttcga ttgaacctgt aacgtcagta
      301 gtacggaagt agaattgtgg acggtagttg ttgaagaatg gagtgtgacg tccaccttct
      361 tctttagtaa ggatgtagac ttcacctttg aatttagtgt gtgggttgat tgaacctggt
      421 ttagcgataa cttgtccacg ttcgatttca tcacgttgaa caccacgaag aaggacacct
      481 acgttatctc cagcaagacc ttcgtcaagt tgtttacgga acatttcaac accagtaaca
      541 actgcttttt gagtttcttc tttgatacca acgatttcga tttcgtcgtt gactttaacg
      601 ataccacggt cgatacgtcc tgaagcaact gtaccacgtc cagtgattga gaatacgtcc
      661 tcgactggaa gaagcaatgg tttgtcagtg tcacgttctg gttctgggat atactcatca
      721 actgtgttca tcaattccat aacgatgtct tcgtatttag agtcaccttc aagagcttta
      781 agtgctgaac cttggataac tggaagatcg tcacctggga agtcgtattc tgacaatagg
      841 tcacggattt ccatttcaac caattcaagc aattcttcgt cgtcaaccaa gtcaactttg
      901 ttcatgaaga cgataaggtg tttaacacca acctgacgtg aaagaaggat gtgctcacga
      961 gtttgtggca ttggtccgtc agttgaagct actacaagga tagctccgtc catttgagca
     1021 gcaccagtga tcatgttttt aacgtagtcc gcgtgtcctg gagcgtcgat gtgagcgtag
     1081 tgacgttttt cagtttcgta ctcaacgtgc gcagtgttga tagtgatacc gcgttcgcgt
     1141 tcttctggag cagcatcgat agacgcatag tctttaggtt ggttaactga tgaaggcaag
     1201 cgacgtgcca aaacagttgt gatagctgca gttagggtag ttttaccgtg gtcaacgtgt
     1261 ccgatagtac caatgttaac gtgtggttta ctacgatcgt atttttcttt tgccatttga
     1321 gtaaaagcct ccaataaaat atattttata gatagacagt aggcaataca gtctaacttt
     1381 ccttactatt ttatcaaatt tcagctaaat tgcaagtgtt ttacaacgtt tttgtgaatt
     1441 attcttggta aaaatttaac taattaatag aaagtactaa atagacttat ccttcaagga
     1501 gttttcagca ttatccatcc actcacttgc tgaaaatgta aacagtctct ggtttatata
     1561 gtaaaatgaa acaagaatag a
//
LOCUS       DAVBCW010000173         1565 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.173, whole genome shotgun sequence.
ACCESSION   DAVBCW010000173 DAVBCW010000000
VERSION     DAVBCW010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.173"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(173..313)
                     /locus_tag="VQ514_002013"
     CDS             complement(173..313)
                     /locus_tag="VQ514_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500728.1"
                     /translation="MKFFWTILAILFIKPIIGIVKFFWMIISFAVQLLFYKILDWFFK
                     LI"
     gene            complement(310..492)
                     /locus_tag="VQ514_002014"
     CDS             complement(310..492)
                     /locus_tag="VQ514_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500729.1"
                     /translation="MNPIKAFAKIYGNYFLTVQGVKVMKTIKKDDHVVVGLGKLFIAD
                     KLMDTARWLIKPEERE"
     gene            complement(494..1277)
                     /locus_tag="VQ514_002015"
                     /pseudo
     CDS             complement(494..1277)
                     /locus_tag="VQ514_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          523 a          324 c          236 g          482 t
ORIGIN      
        1 gatagaactg acgaagtcag taaccatacc tacagcaagg tgaagctgac gtggtttgaa
       61 gagattttcg aagagtataa aatgatttta ctgcttatat tataccatat ggagagagat
      121 taaaaaacta gcagtggagt tcctgctagt tttctgcgac ttggattacc atctagataa
      181 gtttaaagaa ccaatccaat atcttgtaaa acagcaattg aactgcaaaa gaaatgatca
      241 tccaaaagaa tttcacaatc ccaataatcg gtttgataaa aagaatagca agaattgtcc
      301 agaaaaattt cattctctct cctctggctt aatgagccac cgagccgtat ccattagctt
      361 atctgcaata aagagttttc ccagaccaac aacgacatgg tcatctttct ttatcgtttt
      421 catcactttt acaccttgca cggtcaaaaa gtaattacca taaattttag caaaagcttt
      481 gataggattc atattattct cctttgatga tttcagcctc ttttcggatt gtttcaacat
      541 cttcttgata ttgaacttca ctatagttga ctagcttgga taattctttc tgcaagcttt
      601 cccccattgg tttcatagtt gcaaaggtta aaccacctga aatgatacca cccaagatag
      661 gaataaattt ccccattcct ttggccaacc ctcccttggt aagattcaca ccaaatattt
      721 ttaagacttt tttcaaaata gggtaccaaa gcgtctttgt taaagcttta ttaggcactg
      781 ttttcattac ctgtttggca attgttatac caccagcacg tagcaaacaa agcagcggtt
      841 ccattcaccc ctaacattac gcctagataa agcaagaggg tattttgagc atcttcactc
      901 aactcctcac gtgaagccca aagatcctca taaccataaa tataacctaa ttcttgggcc
      961 aatttcagag agaaaccata aaattgagcc acataagctg gaatggtaat tgccatagca
     1021 agccctccag gtaatcctgc caaaacagaa gtcccactcg ctaataaaac attatcccta
     1081 atacaggcat tagccactcg atctaagatt tcttgagata agagagacgt tggaccttgt
     1141 tccaataagg taggaatgtc ctgtggaccc aattctttgg aaaacttatc cactaaaaat
     1201 ttcgaacgat caaccttaac aactggtagt tttaccactt gttgtaatac ttgcatagct
     1261 aagtcttgtt cagccatata caaactcctt atttcttttt tgcaccttat catattatat
     1321 ttcctacaaa aaaagcattt tctaattaga taacattaaa aaatacagaa cccctttttt
     1381 atagtaaaat aaaacaagaa caggacaaat cgatcaggac agtcaaatcg atttctaaca
     1441 atgttttaga agtagaggcg tactattcta gtttcaatat attttaaaag aatatttttc
     1501 catttcaaat tcaacctatt ttcctccacc aaaaaagggc tctataatat ttgtagtggg
     1561 taaat
//
LOCUS       DAVBCW010000174         1448 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.174, whole genome shotgun sequence.
ACCESSION   DAVBCW010000174 DAVBCW010000000
VERSION     DAVBCW010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.174"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            117..1268
                     /locus_tag="VQ514_002016"
     CDS             117..1268
                     /locus_tag="VQ514_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2500730.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
BASE COUNT          359 a          266 c          319 g          504 t
ORIGIN      
        1 ggattcattt tcgaatttaa gaaggagtct acaaaccaat gaggcttctt cttttcttgc
       61 ttatttgata actatcaaaa aagtagaaga ttaagagaaa tatagaaaga gatttcatga
      121 aacaattttt agaacgggcc agcattttgg ctctctccct cgttttgatt acctcctttt
      181 ccatttcgag tgccctacca gccatgtttg actattatca gggttattct aaggaacaaa
      241 ttgagctctt ggtgagcttg ccttcctttg gaatcatgat gatgttactg ctaaatggtt
      301 tcttagaaaa aatatttcct gagcgcttac agattagttt gggcttgctg attttatcat
      361 tgagcggtac agctcccttc tggtaccaag cctatccctt tgtctttgga acacggcttc
      421 tctttggttt gggtcttggg atgatcaatg ccaaggccat ttctattatc agtgaacgct
      481 accaaggaaa aaggcgaatt cagatgttag ggctacgcgc ttctgcagag gtcgttggag
      541 cttctctcat taccttggcc gtcggtcagt tgttggcctt tggttggaca gctatctttc
      601 tagcctatag tgctggattt ttggtgctgc ccctttatct gctctttgtc ccttatggaa
      661 aatcaaagaa agaagtcaag aaaagagcga aggaagcaag tcgtttaact cgagaaatga
      721 aaggcttgat ttttacctta gctatcgaag cggcagttgt agtttgtacc aatacagcta
      781 ttaccatccg tattccaagt ttgatggtgg aaagaggatt gggggatgcc cagttatcta
      841 gttttgttct tagtatcatg cagttgatcg ggattgtggc tggggtgagt ttttctttct
      901 tgatttctat ctttaaagag aaactgctcc tctggtctgg tattaccttt ggcttggggc
      961 aaatcgtgat tgccttgtct tcatccttgt gggtggtagt agcaggaagt gttctggctg
     1021 gatttgccta tagtgtagtc ttgacgacgg tctttcaact tgtctctgaa cgaattccag
     1081 ctaaactcct caatcaagca acttcatttg ctgtattagg ctgtagtttc ggagccttta
     1141 cgaccccatt tgttctaggt gcaattggct tactaactca caatgggatg ttggtcttta
     1201 gtatcttagg aggttggttg attgtaatct ctatctttgt catgtaccta cttcagaaga
     1261 gagcttagga ttgattccta agtttttctt ttgatactca atgaaaatca aagagcatag
     1321 ttgattgagt ttggaatagt acgctgtagc tgctaaaata tttctagaaa ttaatttgac
     1381 ttttctaata gagttattca tatcttattt caatctacta tagaaagcta gccgcaggtt
     1441 gctcaaaa
//
LOCUS       DAVBCW010000175         1424 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.175, whole genome shotgun sequence.
ACCESSION   DAVBCW010000175 DAVBCW010000000
VERSION     DAVBCW010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.175"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            <1..165
                     /locus_tag="VQ514_002017"
     CDS             <1..165
                     /locus_tag="VQ514_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HET2500731.1"
                     /translation="GKCVRTGRKQLLKLSSLYAYSELFSALYSRIRKVNLNLPVPYEP
                     PGRKASLMMH"
     gene            complement(565..1266)
                     /locus_tag="VQ514_002018"
     CDS             complement(565..1266)
                     /locus_tag="VQ514_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HET2500732.1"
                     /translation="MYSGFTHYKYYRAEIIRKVQEPEFWKEVNLSDLEKIRLAIRDLL
                     QFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHYLKTHLDEESISKL
                     YHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREIIGLDRESANRIFSK
                     FLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQLLFQHQLPVLRNI
                     VQIIELINNRAGEAA"
BASE COUNT          482 a          258 c          246 g          438 t
ORIGIN      
        1 ggaaaatgtg ttcgaacagg acgcaagcag ctcctcaaat tgtctagtct ctatgcctat
       61 tccgaattgt tttcagcact ttattctagg attagaaaag tcaacctgaa tcttcctgtt
      121 ccttatgaac cacctggaag aaaagcgtcg ttaatgatgc attaaagaac agtcgagatg
      181 aaaaaatcgt gtgacgcacc aagggaggag tctgcccttt tgaggaaatc tagcgaggaa
      241 aaacgatact ggaacagcag aaagtaaaac tgacctcatg aggaggaaga aagtggctca
      301 tgaggtcagg ggttttgtaa gttacatcta gttgagagag gtatgaatga tttgggttaa
      361 aatcaaagta gctcttttgt taataactcg atcaacaaac atcgtggtac aagtatctac
      421 tttgaaattt atcaaccact taacaactga tactgtattc taggaaaacg atgacattct
      481 tcctaataaa acttctcata tatagcataa atttctactc tttttaattt taaatgagtc
      541 tcagcatggc aatcacttta gaatttaagc cgcttctcca gctcgattat tgataagttc
      601 aatgatttga acaatattac gaagtactgg tagttggtgt tggaagagta gttgaacaga
      661 accataagat ttaaacggct cttgcgttaa cactttcgtc tctaaaaaac cattttctac
      721 aatgtagtct acaatcaatt ttacaaatga aatctgcctg gcattaagat tctcatccga
      781 caaaaattta gagaaaatac gattggcaga ctctctatct aagccaataa tctcacgaac
      841 caatctcgga attgccttat tttcataatg actttggtag tctgctttac tacctaattt
      901 ttcccaaagc aatttttcaa gtgcaagcat atcatcagat gtcaactttt tattatggta
      961 tagcttagaa atggactcct catccagatg agttttcaaa taatgctcaa ctttttcatt
     1021 gtaagaccga agatcgttga cctgcaaaaa tgctgtggtc tcgtgaacag tggagagtat
     1081 acgatcttca aagttaacat agtagggttt acggtctgtt ttatccaaaa actgtaataa
     1141 atctcgaata gcaagacgaa ttttttccaa atcagacaag ttaacttctt tccaaaattc
     1201 aggctcctgt actttcctga taatttcggc tctataatat ttgtagtggg taaatccact
     1261 atagatatta tggagcctat tttattgtag aaaaaaagtc ccataagatc tataatgaaa
     1321 agcgaccaaa caactcatta gaaagattca tatggaacaa ttacatttta tcacaaaact
     1381 actcgatatc aaagacacaa atatccaaat tatagatgtc gtca
//
LOCUS       DAVBCW010000176         1336 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.176, whole genome shotgun sequence.
ACCESSION   DAVBCW010000176 DAVBCW010000000
VERSION     DAVBCW010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.176"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(137..430)
                     /gene="rpmA"
                     /locus_tag="VQ514_002019"
     CDS             complement(137..430)
                     /gene="rpmA"
                     /locus_tag="VQ514_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HET2500733.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(447..791)
                     /locus_tag="VQ514_002020"
     CDS             complement(447..791)
                     /locus_tag="VQ514_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HET2500734.1"
                     /translation="MIQAVFERAEDGELRGAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENY
                     SEFVQTRVITEN"
     gene            complement(807..1121)
                     /gene="rplU"
                     /locus_tag="VQ514_002021"
     CDS             complement(807..1121)
                     /gene="rplU"
                     /locus_tag="VQ514_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HET2500735.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYIEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(1157..1244)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
BASE COUNT          358 a          290 c          237 g          451 t
ORIGIN      
        1 tatacatatt aaaaagtatg gatatcaaaa caaaaaaaca ggctctccga aaactcggaa
       61 agccttattt taagctgttt ctagcttcct cacctttaca tttcaaggtt cgggataaaa
      121 ggttcaatgg acctttttat ttagcgattg ggtaaacaga aacttgtttt ttatcgcgtc
      181 ctttacgttc aaagcgtact acgccttcaa ctttagcgaa caaagtatcg tctccaccac
      241 gaccaacgtt tacacctgga tagatgtgtg taccacgttg acggtaaagg attgatccac
      301 ctgttacagt ttgtccgtca gctgctttag ctccaagacg ttttgcttgt gaatcacgtc
      361 cgtttgatgt agaacctcca ccttttttgt gggcgaaaag ttgcaagttg ttaagagtca
      421 tttttaacat aatgttttcc tccgtgttag ttttctgtga taactctggt ttggacgaac
      481 tcagaatagt tctccgataa gtttgccata cctaagaaaa atgattcaaa gaataactgg
      541 gtcatttctc tctggtgtga aggaagatct tttggtattt caaccatcag atagccacct
      601 tcatcttcgt ttaattctag gattggttca tagcctgcaa atttctcaat agaattgata
      661 aagttaatgg caagcgtaga aaccgatgca cacacgacat ctaagccgta ttcgccactc
      721 tcggcgtgtc cagtaatttc cgcacccctc agctcgccat cttcggctct ctcaaagact
      781 gcttgtatca tgtgttctcc ttaaaattaa gcgttaattg cgttgatgac aacttttgta
      841 tatggttgac ggtgaccttg tttacggtgg ctaccttttt taggtttgta cttgtaagta
      901 acaactttct tttgttttcc ttgtttttca acagttccaa ctacagtagc tccagcaaca
      961 agtggagttc cgacaacagt gttttcacca ccaacaagaa caacttcgtt aaaagtaact
     1021 tcttgaccag cttcaacgtt caatttttca atgtaaactg cttgaccaac ttcaacttta
     1081 acttgttttc cgccagtttt gataattgcg tatgtgctca ttatgcacct cctatgattt
     1141 ttatgggttt ccccgatttt ttgtgaagac tcgcctagca tcgtgggaca aaccacttaa
     1201 acttgaataa tcaagcaacg atgttcgtgc ggttgcacag gatcgtgcat agtcaactct
     1261 tcaagtatag catatctcct attttcttac aagtaataac acctaaaatc aagctttttc
     1321 ttttactttt ttctgc
//
LOCUS       DAVBCW010000177         1329 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.177, whole genome shotgun sequence.
ACCESSION   DAVBCW010000177 DAVBCW010000000
VERSION     DAVBCW010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.177"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(<1..640)
                     /locus_tag="VQ514_002022"
     CDS             complement(<1..640)
                     /locus_tag="VQ514_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2500736.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFDEINERGQTILMVTHSTAAASRAKR"
     gene            1068..1259
                     /locus_tag="VQ514_002023"
     CDS             1068..1259
                     /locus_tag="VQ514_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500737.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFIGTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
BASE COUNT          395 a          258 c          253 g          423 t
ORIGIN      
        1 cacgtttagc tctgctagct gctgctgttg agtgggttac catgagaatg gtttggccgc
       61 gctcattgat ttcatcaaag acatcaagta aggctgcaga tgacttggag tcaagagctc
      121 ctgtcggttc gtccgcaagg agaatttcag gttcagtgat gatggcgcgt gctactgcta
      181 cacgctgttt ctggccacca gaaatctcgt aagggtactt ctcttgtaat tggtttattc
      241 ccagattctc agctgtcacc accaatttct tcatcatctc tgtaataggt cttcttgaca
      301 agacaagcgg aagcaagata ttgtccttta cagatagagt atctagcaag ttaaagtctt
      361 ggaagacaaa gcccaacttt tcacgacgga agctagaggc ctgtgaattt ttaatggttg
      421 cggtgtcagt tccattcaag taaacctgac cacgacttgg tttatccagc atagctagaa
      481 tattgagaag agttgattta ccagaaccag actcacccat gatggcaacg tagtcaccct
      541 tttcgacggt aaagtgaata tccttgaggg cttctacttg gttgccctga aaacgtgttt
      601 tataaatttt ttgaacgtgt tttacatcta aaagtgtcat gagaatctcc tttcttaata
      661 tcccatcaaa tgaaatgtag cttgcatcat agtgcatccc agctgaacgc gctcaatttc
      721 tttgtgactt ggttttctag ttttcttatg taagatttgt ttcatgatgt tactcctttg
      781 ttctttatga gtctagttta catcaaaaaa agaccgcttg ccataaccta accttacaaa
      841 agagacttta atcttacatt tttgtaagat tgggtgaaat ataagcaaaa ttacttagaa
      901 aatgatttga tttcctattt ttaaagttgt aaaatataag tatgaaaagg cttactttgg
      961 aaaggaggaa gcgggaacta tcatttgagt aagcttacct agataacata ccattcaatt
     1021 taacacttgc aagtcgttca acctataagg aaagaaggtg cgctttaatg aaacacaaag
     1081 aacatatact catcggactt ctctatctgc tctctccatt catcggtcag ctcttagttg
     1141 aacacacaca ctttatcggt acagaattta ccggcactgc ttatgttatt tgctggttat
     1201 cagttgttat cagcatccac catttttcca aaaatgtatt gtctcagcag caaaaataga
     1261 ttaaaaaact gagtttaaat tactgctttt ataataaaat gaaataagaa caggacaaat
     1321 cgatcagga
//
LOCUS       DAVBCW010000178         1314 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.178, whole genome shotgun sequence.
ACCESSION   DAVBCW010000178 DAVBCW010000000
VERSION     DAVBCW010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.178"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            223..663
                     /locus_tag="VQ514_002024"
     CDS             223..663
                     /locus_tag="VQ514_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2500738.1"
                     /translation="MKVELQISETYEEEKLIVQAPQPTDKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPTNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            660..1118
                     /locus_tag="VQ514_002025"
     CDS             660..1118
                     /locus_tag="VQ514_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HET2500739.1"
                     /translation="MKKQVFHDAAAGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWAAIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
BASE COUNT          419 a          284 c          244 g          367 t
ORIGIN      
        1 agtaaaatag tggtatactc aataaaaatc aaagagcaaa ctagaaagct aggcgcagac
       61 agctcaaaac actgttttga ggttgtagat aaaactgaca aagtcaacaa ctatcctacg
      121 gcaagacgac gctgacgtgg tttgaagaga tttttgaaga gtattaataa aggtgtacaa
      181 gaagtgagat taattttgta tgaaaaacaa ggagagaaat ttatgaaagt agaattacag
      241 attagtgaga cttacgagga ggaaaagctg attgtccaag cacctcagcc gacagataaa
      301 gttcagaaag tcatcgagtt cgcagaaaat ctggaccaaa gagaaaaaat caaagggaaa
      361 atcgatgatc aggtctatct agttgagatt ggcaagattc agcgcttcta tatcgagaat
      421 cggaaggttc tagcagaaac cgcgagtcag acctacagca ttgatttgcg actctatcag
      481 gttcttaaac tcttgccaac caattttatc caaatttccc aatcagaaat catcaatatc
      541 gattccatct ctcacctcaa gctcacgcca aacggtctgg tagaaatttt cttgaaaaac
      601 gaaagcttca cctactcttc acgccgttat ctaaaaacca tcaaggagaa attagaacta
      661 tgaaaaaaca agtatttcac gatgcagctg ccggtgttct tatcggcctc atcctctcta
      721 tcctcttttc actcatttat gcaccaaata cctacgcacc actaaatccc tactctctca
      781 tcggccaagt gatggatcag catcaggttc acggtgcctt ggtcttgctc tactgcacac
      841 ttatctgggc agccattggt atgctcttca actttggcaa tcgcttattc agccgtgact
      901 ggagcatgct tcgtgccact ctgactcatt tcttccttat gctggctggc tttgtcccac
      961 tagcaactct tgctggttgg ttccctttcc actggatttt ctacctccag ctcattatcg
     1021 agtttgcgat tgtctatctc atcatctggg ctattctcta taaaagagag gctaaaaaag
     1081 tagatcacat caatcaactc ttggagcata gaaaatagga acctcataat aagcaaatcc
     1141 gagaaagaaa cttctcggtt tttctttatc ctgctgcacc ttactatagt gaaatgaaat
     1201 aagaacagga taaatcgatc aggacagtca aatcgatttc taacaatgtt ttagaagcag
     1261 aggtgtacta ttctagtttc aatcctctat atcataacag ttatttcaga tctt
//
LOCUS       DAVBCW010000179         1304 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.179, whole genome shotgun sequence.
ACCESSION   DAVBCW010000179 DAVBCW010000000
VERSION     DAVBCW010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.179"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            <1..702
                     /locus_tag="VQ514_002026"
     CDS             <1..702
                     /locus_tag="VQ514_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HET2500740.1"
                     /translation="IVCDTANTARIDDKRYSQGDFLIKIDHHPNDDVYGDLSWVDTSS
                     SSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFLYPSTTARTLRLAAYLREHNF
                     DFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVILSQKILKQYNVTDAETAAIV
                     GAPGRIDSVSLWGIFVEQADGHYRVRLRSKIHPINEIAKEHDGGGHPLASGANSYSLE
                     ENEIIYQKLKNLLKN"
     gene            756..1040
                     /locus_tag="VQ514_002027"
     CDS             756..1040
                     /locus_tag="VQ514_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HET2500741.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(1097..>1304)
                     /locus_tag="VQ514_002028"
     CDS             complement(1097..>1304)
                     /locus_tag="VQ514_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron permease"
                     /protein_id="HET2500742.1"
                     /translation="PRFEALEQAIASKDLEKVREAFKKMNSTWTINESVVRDNSTAHY
                     GRVETAISFLPSSMETEPTDESGT"
BASE COUNT          388 a          288 c          269 g          359 t
ORIGIN      
        1 atcgtctgtg atacagctaa tactgctcgt atcgatgata agcgctatag tcaaggtgat
       61 tttctcatta agattgacca ccatccaaat gatgatgtat acggtgacct gtcttgggtc
      121 gatactagtt caagtagcgc tagcgagatg attaccctat ttgcccaaac aacccaacta
      181 gccttggcag atcgcgatgc tgagttgctc tttgcaggaa ttgtcggtga tacaggtcgc
      241 ttcctctacc cttctaccac tgcacggact cttcgcctgg ctgcttattt gagagaacat
      301 aactttgact ttgcggctct cactcgcaaa atggacacta tgagctacaa aattgctaaa
      361 ctgcaaggct acatctacga ccatctggaa gtggatgaaa atggtgctgc tcgcgttatc
      421 ctgagtcaga aaatcttgaa acaatacaat gtaaccgatg ctgaaactgc ggccattgta
      481 ggtgcacctg ggcgtattga tagtgttagt ctctggggaa tttttgtgga acaggctgat
      541 ggccactacc gcgttcgctt acgcagtaaa atccatccta tcaatgaaat cgccaaggag
      601 catgatggtg gaggccaccc tctagcaagt ggtgctaatt cctatagcct agaagaaaat
      661 gaaatcatct accaaaagtt aaaaaacttg cttaaaaact gataaaatac ttgccaaact
      721 tttcagaatc tgatagacta gtatggtaac aatctatggc tcgcaaagag accatggcag
      781 aaaggaaata ttgcaaaatg aaaaaagata tccatccaga atatcgccca gttgtcttca
      841 tggacacaac tactggttac caattcctta gcggttcaac aaaacgctct aacgaaacag
      901 ttgagttcga aggcgaaact tacccattga tccgtgtgga aatttcatca gactcacacc
      961 cattctacac tggacgtcaa aagttcactc aagcagatgg acgcgtggat cgtttcaaca
     1021 aaaaatacgg tctcaaataa tgataaaaga acagtttcga ctgttctttt ttgtttcttg
     1081 aaatcaactg ctgttttcat gttccagact catctgtagg ttcggtttcc atgctactag
     1141 gcaggaagga aatagctgtt tcaacacgtc cataatgagc tgtactattg tcacgaacca
     1201 cactttcatt gatggtccaa gtggaattca ttttcttaaa agcttctcgg actttttcca
     1261 aatctttgga ggcaatggcc tgttccaagg cttcaaaacg agga
//
LOCUS       DAVBCW010000180         1190 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.180, whole genome shotgun sequence.
ACCESSION   DAVBCW010000180 DAVBCW010000000
VERSION     DAVBCW010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.180"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            239..>1190
                     /locus_tag="VQ514_002029"
     CDS             239..>1190
                     /locus_tag="VQ514_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HET2500743.1"
                     /translation="MQEHYTPKGKHLTIDNRRLIERWKNENKSNREIAGLLGKAPQTI
                     HNEVKRGTTLQQVRKGLYKKVYSADYAQTVYQFNRKRSVKKLILTKEIREKILHYHKQ
                     KFSPEMMVNKKQVKVGISTIYYWFHNGHLGLTKADMLYPRKRKGVKKQASPNFKPAGK
                     SIEERPDVINLRLENGHYEIDTVLLTKIKNYCLLVLTDRRSRHQIIRLIPNKTAESVN
                     QALTLLLGEHRILSITADNGSEFKRLSEVFPEEHIYYAHAYSSWERGSNENHNRLIRR
                     WLPKGTKKTTPKEVAFIENWINNYPKKCLDYKSPSEFLLGG"
BASE COUNT          413 a          210 c          249 g          318 t
ORIGIN      
        1 agccacctta ccttagtcaa gaattagatg tttcactatg tttgagtaag ttgatgattt
       61 catttgataa caggtttgaa actgttaggc taggtggcca aggctaatca tagccttggt
      121 ttagctgaaa aaacaggttc aagggttcct gttgtcaaat gaaatgtgat ttaaggtata
      181 agaaaacacc tctgtgctat acttgttgtt caccacaaac acaaggaaag gcacagagat
      241 gcaagaacat tataccccaa aagggaaaca tttgacaata gataaccgtc gcttgattga
      301 gcggtggaag aatgaaaata agtccaatcg tgaaattgca ggcttgttag gaaaggcgcc
      361 tcaaacgatt cataatgaag tcaaaagagg tacaacttta caacaagtga gaaaagggct
      421 atacaaaaag gtctattctg ccgattacgc acaaactgtt taccaattca atcgaaaacg
      481 gtcggtgaaa aagttaattt taacaaagga aatcagagag aagatcttac actatcataa
      541 gcaaaaattt tcgcctgaaa tgatggttaa caagaagcaa gtgaaagttg gtatttcaac
      601 catctactac tggtttcata atggtcattt aggattgacg aaggccgaca tgctttatcc
      661 cagaaaaagg aaaggtgtca agaagcaagc tagtccgaac tttaagccgg caggtaaatc
      721 tatcgaagaa cgtcctgacg ttattaatct tcgcttggaa aatggtcatt atgaaattga
      781 taccgtccta ctgactaaga taaaaaatta ttgcctgtta gtcttaaccg accggcggag
      841 cagacaccaa attataaggt taattccaaa taaaactgct gaatctgtca atcaggcgct
      901 tacgttacta ttaggggagc atcgtattct gtccattact gcagataatg gttcggagtt
      961 caaacgattg tctgaggtat ttcctgagga acatatctac tacgcacatg cttactcttc
     1021 atgggagaga ggttcaaatg aaaatcataa tcgattaatt cggagatggt tacctaaagg
     1081 aaccaagaaa acgactccga aagaagtagc ttttatcgaa aattggatta acaactaccc
     1141 taaaaaatgc ttggactaca agtcgccaag tgaatttctt ttgggtggct
//
LOCUS       DAVBCW010000181         1175 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.181, whole genome shotgun sequence.
ACCESSION   DAVBCW010000181 DAVBCW010000000
VERSION     DAVBCW010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.181"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            385..>1175
                     /locus_tag="VQ514_002030"
     CDS             385..>1175
                     /locus_tag="VQ514_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001822067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chordopoxvirus G3 family protein"
                     /protein_id="HET2500744.1"
                     /translation="METSVPVFGNCCLDLPAEAEALVLAEAEALVLAEAEALVDAEAD
                     ALVDAEAEALVDAEADALVDADSDALALVEADVLTEVDALVDADVLAEVDALVDAEAD
                     ALVLAEAEALVDADSDAEILAEADALVDAEAEALVLAEADALVDAEADALVLAEAEAL
                     VDAEADALVLAEAEALVDAEADALVLAEADALVLAEAEALVDAEAEALVDADSEALVD
                     AEADALIDADSEADVLAEAEALVDAEADALVDADSEALVDAEAEA"
BASE COUNT          230 a          265 c          382 g          298 t
ORIGIN      
        1 gcactggtac ttgcggaagc tgaagcgctt gtcgatgctg aggctgaggc agacgttgat
       61 gcggattcag acgcacttgt gcttgctgag gctgacgcgc tggtacttgc tgaagccgaa
      121 gcactggtac ttgctgaagc cgaggcgctt gtcaatgctg aggcagacgt tgatgcggat
      181 tcagacgcac tcgttgatgc tgaggctgaa gcacttgttg aagctgatgt acttgctgat
      241 gtcgacgcgc ttgttgatgc tgaagctgag atacttgctg aagctgaggc actggttgat
      301 gctgaagctg aggcgctggt gcttgctgaa gccgaagcac ttgttgaggc cgattcagac
      361 gctgaggtac ttgctacaga tgacttggaa acttctgtac ctgtatttgg caattgctgt
      421 cttgacttac ctgctgaagc cgaggcgctg gtgcttgctg aggctgaggc gctggtactt
      481 gctgaagccg aggcgcttgt tgatgctgaa gccgacgcac tcgttgatgc ggaagctgag
      541 gcactggttg acgctgaagc cgacgcactc gttgatgcag attcagacgc acttgcgctt
      601 gttgaagccg atgtacttac tgaggtcgac gcgcttgttg atgccgacgt gcttgctgag
      661 gtcgacgcgc ttgttgatgc tgaagctgac gcactggtgc ttgctgaagc cgaggcgctc
      721 gttgatgccg attcagacgc tgagatactt gctgaggctg atgcactcgt tgatgctgaa
      781 gctgaagcac tggtacttgc tgaggctgat gcactggttg atgctgaggc tgacgcactt
      841 gtacttgctg aggccgaggc actcgttgat gctgaagctg acgcactggt gcttgctgaa
      901 gccgaggcgc tcgttgatgc tgaagctgac gcacttgtgc ttgctgaagc tgacgcactt
      961 gtacttgctg aagccgaagc actcgttgat gctgaggctg aagcactggt tgatgccgat
     1021 tcagaggcac tggttgacgc tgaagccgac gcacttattg atgcagattc agaagctgat
     1081 gtacttgctg aagccgaagc gcttgtcgat gctgaggctg atgcactcgt tgatgccgat
     1141 tcagaggcac tggttgacgc tgaagctgag gcaga
//
LOCUS       DAVBCW010000182         1124 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.182, whole genome shotgun sequence.
ACCESSION   DAVBCW010000182 DAVBCW010000000
VERSION     DAVBCW010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.182"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            84..491
                     /locus_tag="VQ514_002031"
     CDS             84..491
                     /locus_tag="VQ514_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2500745.1"
                     /translation="MARYGVYAVIPNPEQKQIVLVQEPNGAWFLPCGEIEAGENHQEA
                     LKRELIEELGFTAEIGTYYGQADEYFYSRHRDTYYYNPAYLYEATSFKEVQKPLENFN
                     HIAWFPIDEAIQNLKRGSHKWAIESWKKQHKIG"
     gene            656..955
                     /locus_tag="VQ514_002032"
     CDS             656..955
                     /locus_tag="VQ514_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HET2500746.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKTSVNEANIKTLYSEKLIGISIPIK"
BASE COUNT          402 a          225 c          189 g          308 t
ORIGIN      
        1 actataataa taacgatact agataatgag gaaaatgcaa tggcaaaccc aactttcgga
       61 gaaaaaaagc gaatacagac tatattgctc gctatggcgt atatgcagtt atccctaatc
      121 ctgaacaaaa acaaattgtt cttgttcaag aaccaaatgg tgcttggttc ctaccatgtg
      181 gagaaattga agcaggtgaa aatcatcagg aagccctaaa gcgtgagttg attgaagagc
      241 ttggtttcac agcagaaatt ggtacctatt acggacaagc tgacgaatat ttctattctc
      301 gtcatcgtga tacctactac tacaatcctg cctacctcta tgaagcaact tctttcaaag
      361 aagtacaaaa gccactagaa aactttaatc atattgcctg gttccctatt gacgaggcta
      421 tccaaaacct taaacgtggt agccataaat gggccattga atcttggaaa aaacagcata
      481 agattggcta atactcttcg aaaataaaat tcaaaccacg tcagcgtcgc cttaccgtac
      541 tcaagtatag cctgcggcta gcttcctagt ttgctctttg attttcattg agtataaatc
      601 aagcgacttt attcaaaaag tgctataata gaattagaaa atcggaggaa atattatgaa
      661 gcttatcaat acgacaaact cacactcgca acttgtaaaa agtcaactag aaagtaccga
      721 tgcaaccctt gttgaggttt attctgcagg gaatacagat gttattttta cccaagctcc
      781 gcttcactat gaaatcctca tctcaaacaa acaccgtgct attcgtgagc ctgaaatcga
      841 aaccattcaa gaatttttct tgaaacgtaa aattgataag acctctgtta atgaagccaa
      901 tatcaagaca ctctactcag agaaattaat tggaatttca atcccaatca agtaagaaaa
      961 ttgagtaaaa actcgatttg agagaaagtc caataagtct ttccataaca aaacgcatag
     1021 taacaagagt ttctacaccc actattatac gtttttatca ttttgaaatc tgctgctaat
     1081 caatgaagaa gcaaagatca aactaggcag ctagttacaa gtta
//
LOCUS       DAVBCW010000183         1112 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.183, whole genome shotgun sequence.
ACCESSION   DAVBCW010000183 DAVBCW010000000
VERSION     DAVBCW010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.183"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(1..449)
                     /locus_tag="VQ514_002033"
                     /pseudo
     CDS             complement(1..449)
                     /locus_tag="VQ514_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
     gene            complement(685..996)
                     /locus_tag="VQ514_002034"
     CDS             complement(685..996)
                     /locus_tag="VQ514_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500747.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYVCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCPFCFSRFNPGCQNHKERYF
                     K"
BASE COUNT          344 a          232 c          186 g          350 t
ORIGIN      
        1 taagaaacca cttcagcgac aaacttgtac tcagggattt tacgacgagc accaaacaac
       61 tgctcatttt cagatggtgt accatagtaa aatcctgtag ccagttcacg ttgggcaacc
      121 ttccacatct cgtccaccaa gtcttgtttg atagcttcaa acttttcagg actttcaaga
      181 taggcatcca cagccgcctt gtagcagttg gttactgtta atacatagtg aatagactcc
      241 atacgtcctt cgatttttag actgtccaca ccattttcaa tcatatctgg aatgtggtca
      301 atcatagaca tgtcaacggc tgacattgaa aattcttctg gaatctcacc ctgcaaactc
      361 ttacgttctt tcccaaatgg catatcgtaa aggtcgtatt tccaacggca tgactgagaa
      421 catccaccac ggttggcatc acgcatactc tagacgcaat tctaatcttt tagactttgc
      481 ataataactg ttaaacagtt gaaaattaac ccagttttaa caagtagtca aaataccaga
      541 taaaggaaaa aaagtcttta aaatcttaaa aacgcatagt atcaggtatt gaacaatctt
      601 gatattatgc gttttattgt gggaagattt acttcatttt ctcttggaat tgagttttct
      661 cctacttcaa aactagatga tttgctattt aaaatagcgt tccttatgat tttggcaacc
      721 tggattaaag cgagaaaaac aaaaggggca acttaagctt tctttatatt gcttatatgt
      781 tagtagtttt agacaaacac cacataaaat aggcttatcc tgtatcaaag ataagggata
      841 cggctcaaaa gggtgatttt ctaatgaatc atgacaccga taacaagcat agtacttttt
      901 acaaacatag cattgtaaag caataatatc cagcttgcta tggtaatgaa tgcatcttgt
      961 ttgcttatcc actaagaaac cctgtgcctc tatcatttcc ttttcctttc ttaagctcga
     1021 actgtgacgc tggttccatc ttccttataa agattgatga gtccttcttt taatgcacga
     1081 accatgtctc cagcttgaac aggttgaggc ac
//
LOCUS       DAVBCW010000184         1081 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.184, whole genome shotgun sequence.
ACCESSION   DAVBCW010000184 DAVBCW010000000
VERSION     DAVBCW010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.184"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            <1..>1081
                     /locus_tag="VQ514_002035"
     rRNA            <1..>1081
                     /locus_tag="VQ514_002035"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          315 a          213 c          325 g          228 t
ORIGIN      
        1 gggacacgtg aaatcccgtc ggaatctggg aggaccatct cccaacccta aatactccct
       61 agtgaccgat agtgaaccag taccgtgagg gaaaggtgaa aagcaccccg ggaggggagt
      121 gaaatagaac ctgaaaccgt gtgcctacaa caagttcgag cccgttaatg ggtgagagcg
      181 tgccttttgt agaatgaacc ggcgagttac gttatgatgc gaggttaagt tgaagagacg
      241 gagccgtagg gaaaccgagt ctgaataggg cgccttagta tcatgacgta gacccgaaac
      301 catgtgacct acccatgagc aggttgaagg tgcggtaaga cgcactggag gaccgaacca
      361 gggcacgttg aaaagtgctt ggatgacttg tgggtagcgg agaaattcca aacgaacttg
      421 gagatagctg gttctctccg aaatagcttt agggctagcg tcgacattag agattcttgg
      481 aggtagagca ctgtttgggt gaggggtcca tcccggatta ccaatctcag ataaactccg
      541 aatgccaatg aattatggtc ggcagtcaga ctgcgagtgc taagatccgt agtcgaaagg
      601 gaaacagccc agaccaccag ctaaggtccc aaaataattg ttaagtggaa aaggatgtgg
      661 ggttgcacag acaactagga tgttagctta gaagcagcta ttcattcaaa gagtgcgtaa
      721 tagctcacta gtcgagtgac cctgcgccga aaatgtaccg gggctaaaac aatttaccga
      781 agctgtggat acctttatag gtatggtagg agagcgttct atgtgtgatg aaggtatacc
      841 gtgaggagtg ctggaacgca tagaagtgag aatgccggta tgagtagcga aagacaggtg
      901 agaatcctgt ccaccgtaag actaaggttt ccaggggaag gctcgtccgc cctgggttag
      961 tcgggaccta aggagagacc gaaaggtgta tccgatggac aacaggttga tattcctgta
     1021 ctagagtatg tagtgatgga gggacgcagt aggctaacta aagcagacga ttggaagagt
     1081 c
//
LOCUS       DAVBCW010000185         1080 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.185, whole genome shotgun sequence.
ACCESSION   DAVBCW010000185 DAVBCW010000000
VERSION     DAVBCW010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.185"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(<1..107)
                     /locus_tag="VQ514_002036"
     CDS             complement(<1..107)
                     /locus_tag="VQ514_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2500748.1"
                     /translation="MQDNYTTKAKHLTIDSRRLIERWKKEGKSNREIASL"
     gene            complement(565..963)
                     /locus_tag="VQ514_002037"
     CDS             complement(565..963)
                     /locus_tag="VQ514_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2500749.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLQIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
BASE COUNT          320 a          208 c          158 g          394 t
ORIGIN      
        1 agagaggcaa tttctctatt tgattttcct tcttttttcc atcgttcgat taagcgacgg
       61 ctatcgattg tcaaatgttt ggcttttgta gtataattgt cttgcatctc tgtgcctttc
      121 ttgtgtttgt ggttgaacaa caagtataac acagaggtgt tttcttatgc ctacaagagt
      181 tttcattatt tccattttct ttcggatttc actctattct gaaaaacttg tgtatattta
      241 ctgaagctag caagtcttac ctgtaaattt aatgaaagca acacaaaatc ctagagggga
      301 atctcggatt aatgtcaatt taaaaagata gagagttttt agtttaaata aattgtttta
      361 aaatatcaac aacatcactt cttttcttaa cctgataagc cttgattcct aattttggag
      421 ctacgattat attgtcctca atatcgtcta gaaagacaca atttctaggt tataactggt
      481 atttatcgat agttactcat atacatcagt ccacctccat acttatgtgc gagcctctct
      541 ttgtattata cctccatact caccttacag attcttttgg taataatatc tttgcctaat
      601 gtagagatag tcttgcaaag aaaaaacttc cttgtagcca tgtttctgat aaaagtccgg
      661 tgcctggaac tgataagtat tgacaaaggc aaaacaacaa tttcgattct tagcttcact
      721 ttctgcctgt tgcaatagtt ttgaaccgat tccttgccct cgcagttcct cttttacaaa
      781 caaatactcg atttgtagcc aatttccaaa agtctctgct atcaaacccg caaggagatt
      841 gcccttttca tcttcgacat aaagattaag tggctcactt tcagcctctt ccctttttga
      901 acggttataa acacgaatca gattccctat ttcttgcgat ttatgtgatt ccttattttc
      961 caatctaaag tataatgaaa tgaaataaaa catgcgcaaa tcgattaagg aatttaatct
     1021 aatttctaat aatgtcttag aaatcaaagt ttactatttt aacttcaatg cactatacat
//
LOCUS       DAVBCW010000186         1065 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.186, whole genome shotgun sequence.
ACCESSION   DAVBCW010000186 DAVBCW010000000
VERSION     DAVBCW010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.186"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            43..396
                     /locus_tag="VQ514_002038"
     CDS             43..396
                     /locus_tag="VQ514_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HET2500750.1"
                     /translation="MILRHLGISPTNDLVAKKIFSNPEITCQFIRDMLDLPAKNVTIL
                     EGSNIHVLPSMPYSAQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWAYLCSQV
                     NQNLEKIRQREGDTH"
     gene            611..727
                     /locus_tag="VQ514_002039"
     CDS             611..727
                     /locus_tag="VQ514_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HET2500751.1"
                     /translation="MVGVFRQQALYPATATSHYPSKSTAGLQELVRGGQENV"
     gene            720..953
                     /locus_tag="VQ514_002040"
     CDS             720..953
                     /locus_tag="VQ514_002040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500752.1"
                     /translation="MFSQLHMREEQVLLAQDYALETARAEGLEQGLERGKVEGRAERK
                     LFAFLDIVRQGLLTSEVASQQLGMSVSEFEALL"
BASE COUNT          335 a          214 c          234 g          282 t
ORIGIN      
        1 tgttcacatc ttatttcaat tcactataga aggaggaaaa ggatgattct cagacatctg
       61 ggaattagcc cgaccaatga cttggttgct aagaaaattt tcagcaaccc agaaatcact
      121 tgtcaattta tccgcgatat gctggacttg ccagccaaaa atgtgactat tttggaggga
      181 agtaacattc atgtcttgcc ttccatgccc tactcagcgc aggattttta taccagtata
      241 gacgtcttgg cggagttgga taacggtact caagtaatta ttgagattca agtccatcat
      301 cagaattttt tcatcaatca cttgtgggct tacctgtgca gtcaggttaa tcaaaatctt
      361 gaaaaaattc gtcagcgaga aggtgatact cactagatct acaaacacat cgctcctgtt
      421 tacgtcattg ctatcgtgga tagtaattat ttctcagatg acctggcttt tcatagcttt
      481 agtatgcgcg aagacacaac aggtgaggta ttggcgatta ccaacaatgg acaggaaaac
      541 catctggtta agatggcatt cttggaatta aaaaatacag agaaaccagc aaagacaagg
      601 ttcgcaagcc atggttggag tttttcggca acaagccctt tacccagcaa ccgcaacgag
      661 ccattaccca agcaaatcaa ctgctggact acaagagctg gtccgaggag gacaggaaaa
      721 tgtttagtca actacatatg cgagaagaac aagtcttgtt agcacaggac tatgccttgg
      781 aaactgctag ggctgaaggc cttgaacaag gactagagcg tgggaaagtt gaaggaaggg
      841 cagaaaggaa actttttgcc ttcctagaca tagtacgcca aggtcttctg acttctgagg
      901 ttgccagcca gcaattaggt atgtcagtat ctgaatttga ggcactgttg taaaatggct
      961 ccataatatc catagtgggt aaatccccta tggatattat ggagcctatt tttgtgtaga
     1021 aaaaaagtcc catatgacct ataatgaaaa gcgacaaaac aactc
//
LOCUS       DAVBCW010000187         1054 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.187, whole genome shotgun sequence.
ACCESSION   DAVBCW010000187 DAVBCW010000000
VERSION     DAVBCW010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.187"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            <1..555
                     /locus_tag="VQ514_002041"
     CDS             <1..555
                     /locus_tag="VQ514_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2500753.1"
                     /translation="KDETTSYPIYEIPEAWRYIKFASLVNFRIGKTPPRSEATFWGTE
                     IPWVSISDMPISGYVTNTRESISKLALKSKKIDISPKGTLLMSFKLSIGKVAILDIPA
                     THNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLNSTSISELLIPIS
                     NHEEMKRIISKVDLLFQKVSQLFE"
     gene            complement(503..1048)
                     /locus_tag="VQ514_002042"
     CDS             complement(503..1048)
                     /locus_tag="VQ514_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2500754.1"
                     /translation="MNWVVIKIKDIFSINTGLSYKKGDLSINNKGVRIIRGGNIKPLE
                     FSLLDNDYYIDTQFISSEQVYLKHNQLITPVSTSLEHIGKFARIDKDYDGVVAGGFIF
                     QLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQALYNIPKTTLSELLIPLAPFE
                     EQELITQKVEKLFEKVNQLWK"
BASE COUNT          380 a          165 c          153 g          356 t
ORIGIN      
        1 aaagacgaaa caacctctta tcctatatat gaaatcccag aagcatggag atatattaaa
       61 tttgctagcc tagttaactt cagaatagga aaaactcctc cacgcagtga agctactttt
      121 tggggaactg aaataccttg ggtatctata tcagatatgc ctatatctgg ttatgtaact
      181 aatacgagag aatctatttc taaattagca ttaaaatcaa agaaaataga catttcacct
      241 aaaggaactt tattaatgag ttttaaatta tctattggaa aagttgccat attggatatt
      301 cctgccactc ataatgaagc cattatatcg atattcccct atgctaataa agaaaatatt
      361 atcagagatt acttaatgat atttttgcca cttatctcta ctttaggtga ttcaaaagat
      421 gctataaaag ggaaaacatt aaatagtacc agtatctccg aattattaat ccctatttct
      481 aatcatgaag agatgaaaag aatcatttcc aaagttgatt tactttttca aaaagtttct
      541 caactttttg agtaataagt tcctgttcct caaaaggagc taacggaatt aatagctcgc
      601 ttagtgtagt tttaggaata ttatataaag cttgacctga tagtttagtt attgctttca
      661 attgtttata aaataacgga gaggacaagt taaataatag aaattttgaa ataatctctg
      721 aactttcgaa tggtgttaat tggaaaataa atccaccagc cacaacacca tcatagtctt
      781 tatcgattct tgcaaacttt ccaatatgtt ctaaagaggt tgatacaggt gttattagct
      841 gattatgttt taaataaact tgctcagagg agatgaattg tgtatcaatg tagtaatcat
      901 tatccaacag agaaaattct aaaggcttaa tattaccacc acgtataatt ctaacacctt
      961 tattattaat gcttaaatcg cccttcttgt aagaaagacc tgtatttatt gaaaaaatat
     1021 cttttatttt tataacaacc caattcatag gtat
//
LOCUS       DAVBCW010000188         1035 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.188, whole genome shotgun sequence.
ACCESSION   DAVBCW010000188 DAVBCW010000000
VERSION     DAVBCW010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.188"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(<1..990)
                     /gene="ltrA"
                     /locus_tag="VQ514_002043"
     CDS             complement(<1..990)
                     /gene="ltrA"
                     /locus_tag="VQ514_002043"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HET2500755.1"
                     /translation="MSKLLDKILSRDNMLEAYNQVKSNKGSAGIDGITIEEMDGYLRQ
                     NWRLTKELIKQRKYKPQPVLRVEIPKPNGGIRQLGIPTVMDRMIQQAIVQVISPICEP
                     HFSDTSYGFRPNRSCEKAIMKLLEYLNDGYEWIVDIDLEKFFDTVPQDRLMSLVHNII
                     EDGDTESLIRKYLHSGVIINGQRYKTLVGTPQGGNLSPLLSNIMLNELDKELEKRGLR
                     FVRYADDCVITVGSEAAAKRVMYSVSRFIEKRLGLKVNMTKTKITRPRELKYLGFGFW
                     KSSDGWKSRPHQDSVWRFKLKLKKLTQRKWSIDLTRRIEQLNLSIRGWINYFSL"
BASE COUNT          270 a          226 c          183 g          356 t
ORIGIN      
        1 caatgagaaa tagtttatcc atcctcgaat agacaaattc agttgctcaa tacgtcttgt
       61 taggtctata ctccatttcc tctgtgttag tttcttcaat ttaagcttaa atctccaaac
      121 actatcttga tgtggacgac ttttccaacc atctgatgat ttccagaacc cgaaacctag
      181 atatttcaac tctcttggtc tggtaatctt agtcttggtc atgtttactt tcagacctag
      241 ccgtttctca ataaaacgac tgactgaata catcacacgc ttagcggctg cctcgcttcc
      301 gaccgtaatc acacaatcgt ctgcgtagcg aacaaatcga agccctctct tttctaattc
      361 cttgtccaat tcattaagca tgatattgga taagagagga gataaatttc ctccctgtgg
      421 cgtgccaact agcgttttat aacgttgacc gttaatgata acacctgaat gaagatattt
      481 acgaatcaag gattccgtat ctccgtcttc gataatgtta tgtactaagg acatcaatct
      541 atcttgagga actgtatcga aaaatttctc taggtctatg tccactatcc actcatagcc
      601 gtcatttaag tactctaaga gcttcatgat ggctttttca catgacctat ttggtctgaa
      661 tccatagctc gtatctgaga aatggggttc acaaataggg ctaataactt gaacaatggc
      721 ctgttgaatc attctatcca taactgttgg aattcctagt tggcggatgc ctccgtttgg
      781 tttgggaatc tcaactctaa gaactggttg aggcttatat tttctctgtt ttatcagttc
      841 cttagtcagg cgccagtttt gtctgagata gccatccatc tcttcgatag taattccatc
      901 aatcccagct gagcctttat tagattttac ttgattgtag gcttcaagca tattgtcgcg
      961 tgataatatt ttatctagca attttgacat gtgcctattc ctttcttgtt ttggtgcctg
     1021 agggacatct tatat
//
LOCUS       DAVBCW010000189         1005 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.189, whole genome shotgun sequence.
ACCESSION   DAVBCW010000189 DAVBCW010000000
VERSION     DAVBCW010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.189"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(34..228)
                     /locus_tag="VQ514_002044"
     CDS             complement(34..228)
                     /locus_tag="VQ514_002044"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500756.1"
                     /translation="MSSKKHPLGEKRNHTGSKLRQGFWMVFRLGVNNWGFKEIENKKP
                     SKNSIAGENEIILNENQRAN"
     gene            290..>1005
                     /locus_tag="VQ514_002045"
     CDS             290..>1005
                     /locus_tag="VQ514_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSpn5 family transposase"
                     /protein_id="HET2500757.1"
                     /translation="MNSLPNHHFQNKSFYQLSFDGGHLTQYGGLIFFQELFSQLKLKE
                     RISKYLVTNDQRRYCRYSDSDILVQFLFQLLTGYGTDYACKELSADAYFPKLLEGGQL
                     ASQPTLSRFLSRTDEETVHSLRCLNLELVEFFLQFHQLNQLIVDIDSTHFTTYGKQEG
                     VAYNAHYRAHGYHPLYAFEGKTGYCFNAQLRPGNRYCSEEADSFITPVLERFNQLLFR
                     MDSGFATPKLYDLIEKTGQY"
BASE COUNT          277 a          218 c          172 g          338 t
ORIGIN      
        1 aaacagtgtt ttgagcagcc tgcagctagt ttcctagttt gctctttgat tttcattgag
       61 tattatttca ttttctcctg caattgaatt cttgctcggc tttttgtttt ctatttcttt
      121 aaaaccccaa ttattcaccc ctaatctaaa aaccatccag aatccttgcc ttagcttaga
      181 tcctgtatgg tttctttttt cacccaatgg gtgtttttta ctagacaaaa aagaatttcc
      241 cctttatggt ataagtgtag aaaaaaacac aaaaagaaag gaaactcaca tgaacagttt
      301 accaaatcat cacttccaaa acaagtcttt ttaccaacta tctttcgatg gaggtcattt
      361 aacccagtat ggtggtctta tcttttttca ggaacttttt tcccagttga aactaaaaga
      421 gcggatttct aagtatttag taacgaatga ccaacgccgc tactgtcgtt attcggattc
      481 agatatcctt gtccagttcc tctttcaact gttaacaggc tatggaacgg actatgcttg
      541 taaagaattg tcagctgatg cctactttcc aaaattgttg gaaggagggc agcttgcttc
      601 acagccaacc ttatcccgtt ttctttccag aactgacgag gaaacagtcc atagtttgcg
      661 atgcctcaac cttgaattgg tcgaattctt tttacagttt caccagctaa accaactcat
      721 tgtagatatc gattctaccc atttcacaac ttatggcaag caagaaggtg ttgcttataa
      781 cgcccactat cgtgctcatg gctatcatcc tctttatgct ttcgagggga agacaggtta
      841 ttgtttcaat gcccagcttc gtcctggtaa tcgttattgt tctgaagagg cagacagctt
      901 tatcacacct gttttagaac ggtttaatca acttctcttt cgaatggata gtggctttgc
      961 gacccccaaa ttatacgatt taattgaaaa aacagggcaa tacta
//
LOCUS       DAVBCW010000190          954 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.190, whole genome shotgun sequence.
ACCESSION   DAVBCW010000190 DAVBCW010000000
VERSION     DAVBCW010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..954
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.190"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(<1..337)
                     /locus_tag="VQ514_002046"
     CDS             complement(<1..337)
                     /locus_tag="VQ514_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002202361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2500758.1"
                     /translation="MDSASIIAHTIHQKFNLKVPNYRQEEDWARMGLPITRKEIANWH
                     IKASQYYLESLYNLLREKLLEQPLLHADETSYRVLESDSQLTYYWTFLSGKSEKQGIT
                     LYHHDQCRSG"
     gene            complement(345..650)
                     /locus_tag="VQ514_002047"
     CDS             complement(345..650)
                     /locus_tag="VQ514_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HET2500759.1"
                     /translation="MFPVETEEITYKRKKSKGKRQALLAQFDSEEVHHQVEESICSDC
                     QGDLKEIGATLQRQELVFIPAQLKRIDHIQHAYKCQACSDKNPSDKIVKAPIPKAPL"
     gene            complement(637..831)
                     /locus_tag="VQ514_002048"
     CDS             complement(637..831)
                     /locus_tag="VQ514_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2500760.1"
                     /translation="MKIIQQQSATIDSLTNALALLREQVAYLTQKLYGKSSEKSVCPS
                     GQLNLFEEESPSEKDGDVPS"
BASE COUNT          259 a          185 c          188 g          322 t
ORIGIN      
        1 aaccacttcg acactgatca tggtggtaaa gcgtaatccc ttgtttctct gatttacctg
       61 acaaaaaagt ccaatagtaa gtcagctgac tatcactttc taagacccga taagaggttt
      121 catctgcatg aagaagaggt tgttctaaca acttttctcg taaaaggtta taaagggact
      181 ctaaatagta ttgactcgcc ttgatatgcc aattagcaat ttccttacgt gtgattggta
      241 aacccatcct agcccaatct tcttcttggc gataattagg taccttcaga ttaaacttct
      301 gatggatggt gtgagcgata atagaagctg agtcaatagt ctcgttataa aggggcttta
      361 ggaataggag ctttcacaat tttatcactc ggatttttat cactgcatgc ttggcatttg
      421 taagcgtgtt ggatatgatc tatgcgtttt aattgcgcag gaataaagac taattcttgt
      481 cgttgaaggg ttgctccaat ctcttttaga tctccctgac aatcagagca aatgctctct
      541 tctacttgat gatgaacttc ttctgaatca aattgggcaa gaagagcttg gcgtttccct
      601 ttagatttct tacgtttgta ggtaatttcc tctgtttcaa ctgggaacat ctccatcttt
      661 ctcagatgga gactcttcct caaaaaggtt gagttgtcca gaggggcaaa cacttttctc
      721 agaggatttt ccatagagct tttgagtcag ataagccact tgttcacgaa gaagggcaag
      781 tgcattggtg agactatcaa ttgtagcact ctgttgttga atgattttca atcttcttcc
      841 atgggcttcc cctccttgtt ttctaccttt attatactaa aaagaaagtc ctcatttcaa
      901 tagaaatcac gactttctga tggatttatt tttggagtga tagaaaagtc cttc
//
LOCUS       DAVBCW010000191          922 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.191, whole genome shotgun sequence.
ACCESSION   DAVBCW010000191 DAVBCW010000000
VERSION     DAVBCW010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.191"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            122..590
                     /locus_tag="VQ514_002049"
                     /pseudo
     CDS             122..590
                     /locus_tag="VQ514_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817843.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          336 a          139 c          172 g          275 t
ORIGIN      
        1 gagaagagga ttgccatctt ctccattttt atgtgcagag ttatagtggc ttgatgctgg
       61 gaaagtacac tgtgactgct aaaacattcc tagaagctgc tttgatttcc ctaatctatt
      121 tatgcaaatt ttatgttatt ttactataac agttgttgct aagccaaata atagtgggga
      181 agttcattta gacgtaagca ttgaagataa tcagggaggt agtgggtata atttcagttc
      241 tgtttcaagt agctcacaaa cagctaaata tgaaggaact ggttataata acaattcatc
      301 attatatata acgattgata aaacgtctga tgcaacagct cttttgaaat taaagttgaa
      361 taatgttgat aatcaacctg ctactgaagt tcctagttca ggaattactg taaaattaaa
      421 tgctaaagat aatgctggaa actggacaag tgcttcgaat aaagacaagt gcttcgaata
      481 aaaaagaaat aacagtaaaa attgtttctg ctaaaccgac atatccagac aaaatcttag
      541 tgaaaaatcc tgataatata aaagatacag aaaaaaatgc cattattgaa aaattgaaag
      601 aggcaaataa aaatcatcca gcaggagctc caacctttgc taaaggtgaa ggagagcatg
      661 caaatgatat tgtagcaact tattcagatg gtacaactta ttatgtaccg ttaaatgatg
      721 tgacaaaata tgcgaggtag tggctgtacc actcacttat tcacctcccc gtgatttgta
      781 gtagtgatag gctttctcac tattattata aaacaaaata aagatcacaa cactttttca
      841 ttctgtgttg tgccttgagt gaaacgaaag gaatgaatta taaatatgaa aagtatagtc
      901 actagcatag atgagcgctt gc
//
LOCUS       DAVBCW010000192          753 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.192, whole genome shotgun sequence.
ACCESSION   DAVBCW010000192 DAVBCW010000000
VERSION     DAVBCW010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.192"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            6..>753
                     /locus_tag="VQ514_002050"
     CDS             6..>753
                     /locus_tag="VQ514_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001856522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chordopoxvirus G3 family protein"
                     /protein_id="HET2500761.1"
                     /translation="MLAEAEALVLAEADALVDADSEALVDAETEALVDADSDALVLAE
                     AEALVDAEAEALVDAEADALVDADSDAEVLAEAEALVDAEAEALVDAETEALVLAEAE
                     ALVLTEAEALVLAEAEALVDADSEALVDAETEALVDAEADALVLAEAEALVDADSEAD
                     VLAEAEALVLAEADALVDAEAEALVLAEAEALVLAEAEALVLAEADALVDADADALVD
                     ADSDAEILVDAEAEALVDAEAEALVDAEADA"
BASE COUNT          157 a          165 c          244 g          187 t
ORIGIN      
        1 cactggtgct tgctgaagcc gaggcgctag tgcttgctga agccgacgca ctcgttgatg
       61 cggattcaga ggcactggtt gacgctgaga ctgaagcact cgttgatgct gactcagacg
      121 cactggtact tgcggaagcc gaagcactgg ttgatgctga agccgaggcg cttgtcgatg
      181 cggaagctga tgcacttgtt gatgccgatt cagacgctga ggtgcttgct gaagctgagg
      241 cgctcgttga cgctgaagcc gaagcactgg ttgatgctga aactgaggca cttgtgcttg
      301 ctgaagccga agcactggta cttactgaag ctgaggcact cgtacttgct gaagccgagg
      361 cacttgttga tgcggattca gaggcactgg ttgacgctga gactgaagca ctcgttgatg
      421 ctgaagccga cgcactggta cttgctgaag ccgaggcact tgttgatgcg gattcagaag
      481 ctgatgtgct tgctgaagct gaggcgctag tacttgctga agctgatgca ctggttgatg
      541 ctgaggctga agcacttgta cttgctgagg ctgaggcgct tgtacttgct gaggctgaag
      601 cactggtgct tgctgaagct gatgcactcg ttgatgcgga tgctgacgca ctggttgatg
      661 cagattcaga cgctgagata cttgttgatg ctgaagctga agcacttgtt gacgctgagg
      721 ctgaagcact tgttgatgct gaagctgatg cac
//
LOCUS       DAVBCW010000193          748 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.193, whole genome shotgun sequence.
ACCESSION   DAVBCW010000193 DAVBCW010000000
VERSION     DAVBCW010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.193"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(<1..>748)
                     /locus_tag="VQ514_002051"
     CDS             complement(<1..>748)
                     /locus_tag="VQ514_002051"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500762.1"
                     /translation="ALVDAEAEALVLAEADALVLAEADALVLAEADALVDADSDALVD
                     ADSDAEVLAEADALVLAEADALVLAEAEALVLAEAEALVLADSDALVLAEAEALVEAE
                     ADALVDAEADALVLAEADALVDADSEALVLAEADALVDAEAEALVLAEADALVLAETE
                     ALVLAEADALVDAEAEALVLAETEALVLAEAEALVDAEAEALVLAEAEALVDADSDAE
                     VLAEADALVDAEAEALVLAEADALVLAEADA"
BASE COUNT          188 a          246 c          174 g          140 t
ORIGIN      
        1 gtgcgtcggc ttcagcaagc acaagtgcgt cagcctcagc aagtaccagc gcctcagcct
       61 cagcatcaac cagtgcgtcg gcttcagcaa gtacctcagc gtctgaatcg gcatcaacaa
      121 gtgcttcagc ttcagcaagt accagcgcct cagcttcagc atcaaccagt gcttcggctt
      181 cagcaagcac cagtgcctca gtctcagcaa gcacaagcgc ctcagcttca gcatcaacca
      241 gtgcgtcagc ctcagcaagt accagtgcct cagtctcagc aagcaccagt gcgtcggctt
      301 cagcaagcac aagcgcctca gcttcagcat caaccagtgc gtcagcctca gcaagtacca
      361 gtgcctctga atccgcatca acaagtgcat cggcttcagc aagcaccagt gcgtcagctt
      421 ccgcatcaac gagtgcgtcg gcttcagcct caaccagcgc ctcagcctca gcaagtacaa
      481 gtgcatcaga gtcagcaagt accagtgctt cggcttcagc aagtactagc gcctcagctt
      541 cagcaagtac cagtgcgtca gcttcagcaa gtaccagtgc gtcggcctca gcaagtacct
      601 cagcgtctga atcggcatca acgagtgcgt ctgaatccgc atcaacaagt gcgtcagctt
      661 cagcaagtac aagtgcgtca gcttcagcaa gtacaagtgc gtcagcttca gcaagtacta
      721 gcgcctcagc ctcagcatca acgagtgc
//
LOCUS       DAVBCW010000194          719 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.194, whole genome shotgun sequence.
ACCESSION   DAVBCW010000194 DAVBCW010000000
VERSION     DAVBCW010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.194"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            298..447
                     /locus_tag="VQ514_002052"
     CDS             298..447
                     /locus_tag="VQ514_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HET2500763.1"
                     /translation="MGDQPVQDTETSSALILSHYLDEQDLSEKLKSDLQWFELENKLL
                     NLWEY"
     gene            435..>719
                     /locus_tag="VQ514_002053"
     CDS             435..>719
                     /locus_tag="VQ514_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500764.1"
                     /translation="MGVLGYYEAFVPYVSNQYKNQAEEEGKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEKLGKLKPITINYNGKSEVIDSKEKLQELMNKAVKDEVAQ"
BASE COUNT          277 a           89 c          140 g          213 t
ORIGIN      
        1 aagagcttgg caacaggctc tttttgtatt gcgtttatta agaaaaacaa aaaacatcga
       61 tatacattta actaaaaatt attgtatgtt atctccctta attaggaatg ataagggaat
      121 aactagaaag atttgtgaat acaaactatt tctgatatac aaaatataca gtaataatga
      181 atgatgggag atgggatgaa agaatttcaa tttgagagaa agccgcgttt ttctttgagg
      241 ccatatgcaa taggagcttg ttaggtcttg ctaggaacga gtttattttt tgctggtatg
      301 ggtgatcagc ctgtacagga tacagaaacg agttcagcac taattttaag tcattatttg
      361 gatgagcagg atttatctga aaagctgaaa tctgatttgc aatggtttga attagaaaac
      421 aagcttttga acttatggga gtattaggtt actatgaagc atttgttcct tatgtttcaa
      481 atcaatacaa aaaccaagct gaagaagaag gcaaaccgct atctgataaa tatattttcg
      541 aaaaaatatt aggaaaaaca tatgcagcct tcaaaaaaga tcaaattaat gagcgtgtcg
      601 agaaattagg taagttaaaa cctattacaa taaattacaa cggaaaatca gaagtaattg
      661 atagtaaaga aaaattacaa gagcttatga ataaagccgt taaagacgaa gtggctcaa
//
LOCUS       DAVBCW010000195          707 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.195, whole genome shotgun sequence.
ACCESSION   DAVBCW010000195 DAVBCW010000000
VERSION     DAVBCW010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.195"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            <1..71
                     /locus_tag="VQ514_002054"
     rRNA            <1..71
                     /locus_tag="VQ514_002054"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            123..195
                     /locus_tag="VQ514_002055"
     tRNA            123..195
                     /locus_tag="VQ514_002055"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:156..158,aa:Ala,seq:tgc)
     gene            320..>707
                     /locus_tag="VQ514_002056"
     rRNA            320..>707
                     /locus_tag="VQ514_002056"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          220 a          122 c          203 g          162 t
ORIGIN      
        1 tgggatagat gattggggtg aagtcgtaac aaggtagccg tatcggaagg tgcggctgga
       61 tcacctcctt tctaaggata aggaactgcg cattggtctt gtttagtctt gagaggtctt
      121 gtggggcctt agctcagctg ggagagcgcc tgctttgcac gcaggaggtc agcggttcga
      181 tcccgctagg ctccattggt gagagatcac caagtaatgc acattgaaaa ttgaatatct
      241 atatcaaata gtaacaagaa aataaaccga aaacgctgta gtattaataa gagtttatga
      301 ctgaaaggtc agaaaataag gttaagttaa taagggcgca cggtggatgc cttggcacta
      361 ggagccgacg aaggacgtga caaacgacga tatgccttgg gtagctgtaa gtaagcgatg
      421 atccagggat ttccgaatgg gggaacccaa caggtaatac ctgttaccca catctgttaa
      481 ggatgtgagg aggaagacgc agtgaactga aacatctaag tagctgcagg aagagaaagc
      541 aaaagcgatt gccttagtag cggcgagcga aacggcagaa gggcaaaccg aagagtttac
      601 tcttcggggt tgtaggactg caatgtggac tcaaagatta tagaagaatg atttgggaag
      661 atcagccaaa gagagtaata gcctcgtatt taaaatagtc tttgtac
//
LOCUS       DAVBCW010000196          705 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.196, whole genome shotgun sequence.
ACCESSION   DAVBCW010000196 DAVBCW010000000
VERSION     DAVBCW010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.196"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(<1..>705)
                     /locus_tag="VQ514_002057"
     rRNA            complement(<1..>705)
                     /locus_tag="VQ514_002057"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          145 a          227 c          145 g          188 t
ORIGIN      
        1 ggtaaggttc ttcgcgttgc ttcgaattaa accacatgct ccaccgcttg tgcgggcccc
       61 cgtcaattcc tttgagtttc aaccttgcgg tcgtactccc caggcggagt gcttaatgcg
      121 ttagctacgg cactaaaccc cggaaagggt ctaacaccta gcactcatcg tttacagcgt
      181 ggactaccag ggtatctaat cctgtttgct ccccacgctt tcgagcctca gcgtcagtta
      241 caagccagag agccgctttc gccaccggtg ttcctccata tatctacgca tttcaccgct
      301 acacatggaa ttccactctc ccctcttgca ctcaagttaa acagtttcca aagcctacta
      361 tggttaagcc acagccttta acttcagact tatctaaccg cctgcgctcg ctttacgccc
      421 aataaatccg gacaacgctc gggacctacg tattaccgcg gctgctggca cgtagttagc
      481 cgtccctttc tggtaagata ccgtcacagt gtgaactttc cactctcaca ctcgttcttc
      541 tcttacaaca gagctttacg atccgaaaac cttcttcact cacgcggcgt tgctcggtca
      601 gacttccgtc cattgccgaa gattccctac tgctgcctcc cgtaggagtc tgggccgtgt
      661 ctcagtccca gtgtggccga tcaccctctc aggtcggcta tgtat
//
LOCUS       DAVBCW010000197          704 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.197, whole genome shotgun sequence.
ACCESSION   DAVBCW010000197 DAVBCW010000000
VERSION     DAVBCW010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.197"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            77..>704
                     /locus_tag="VQ514_002058"
     CDS             77..>704
                     /locus_tag="VQ514_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2500765.1"
                     /translation="MLYVGIDIAKNKHDVTALNVQGKTVLKPLTFSNNKAGFELLDLS
                     LRQLNQDCLIALKLLSDPNREQFQHDNRQVDLKILARHIHRLKKKQSDWKVQYTRCLD
                     IIFPELDKIVGKHSEYTYQLLTRYPNPQKRIEAGFDKLIEIKRLTASKIQDILSVAPR
                     SIGTTSPAREFEIIEIIKHYKRLIDKAETCVNDLMAEFNSVITTVTGIG"
BASE COUNT          226 a          152 c          130 g          196 t
ORIGIN      
        1 tctcgtctaa ggtaacaagg caaaaagttc gtggataacc aagtagactt gaaatactat
       61 atctggaggt actcccatgc tttatgtggg cattgatatc gctaaaaata aacacgatgt
      121 tacagccttg aatgttcaag gaaaaactgt tcttaaacca ctcacttttt caaataataa
      181 agctggtttt gaactcttag atctgtctct tcgacagctc aaccaagact gtctcatcgc
      241 tcttaaactt ctttctgacc ccaatcgtga acaatttcaa cacgataatc ggcaagtaga
      301 cctaaaaata ctggctagac atattcatcg tctcaagaaa aaacagtctg attggaaagt
      361 acaatacact cgttgtcttg atatcatctt tcctgagttg gataaaatcg ttggaaagca
      421 ttcagaatat acctaccaac tcttgacgcg ctaccctaat cctcagaaaa ggattgaggc
      481 aggatttgat aagctgatag aaattaagcg attgaccgct tctaaaattc aggatatcct
      541 ctcagtcgca cctcgttcta tcggaacaac atctcctgct cgtgaattcg aaatcatcga
      601 aatcatcaaa cattacaaga ggctcattga caaggcggaa acatgtgtca atgacttgat
      661 ggctgagttc aactcagtca tcacgacggt tactgggatt gggg
//
LOCUS       DAVBCW010000198          700 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.198, whole genome shotgun sequence.
ACCESSION   DAVBCW010000198 DAVBCW010000000
VERSION     DAVBCW010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.198"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(196..>700)
                     /locus_tag="VQ514_002059"
     CDS             complement(196..>700)
                     /locus_tag="VQ514_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2500766.1"
                     /translation="GELTILQILYGSSLISFLWMYGQRKQAHKVNMKSRMKWLGVEFV
                     SLLIISLCFSLIHAQGSTNQANLIGLQHQIPWFSFLLFLINASMVEEFLYREILWNLV
                     RKLDIRVALTSVLFALAHHTGTILAWCLYVSLGMFLGMVRYKSDLWGSMGLHLVWNLL
                     VYSLLLF"
BASE COUNT          252 a          150 c           96 g          202 t
ORIGIN      
        1 ctcctcctat ttaaattttt gggattaagt actttatcca ccctcattat actctctcca
       61 ccagtaaaat gcaagcaatt atacaatgtt gtcacataga aaataatgtt tccgtaactt
      121 ttcaaagtaa cttccatctc tctcccaaaa ctggaagtta gttttagaag ttacctaaaa
      181 atcaggtcac ctattttaaa aaagcagcaa actataaact agtaggttcc acaccaaatg
      241 tagtcccata ctgccccata agtcagattt atagcgcacc atacctaaaa acatcccaag
      301 tgaaacatac aaacaccaag ctagaatggt tcctgtatga tgtgctaagg caaataaaac
      361 acttgtcaaa gcaactctga tatctaattt tctgaccaaa ttccataaaa tttctcgata
      421 cagaaattct tcaaccatac tcgcattgat taagaacaat aaaaatgaaa accaaggaat
      481 ttgatgttga aggccaatta agtttgcttg attcgtgctt ccttgagcat gaatcagact
      541 aaaacataga cttataatca gtaggctaac aaattcaaca ccaagccatt tcatcctaga
      601 tttcatattg accttatgcg cttgtttgcg ttggccatac atccataaaa aagaaatgag
      661 tgacgaacca tagagaatct gtagtatagt taactcaccg
//
LOCUS       DAVBCW010000199          692 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.199, whole genome shotgun sequence.
ACCESSION   DAVBCW010000199 DAVBCW010000000
VERSION     DAVBCW010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.199"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(74..147)
                     /locus_tag="VQ514_002060"
     tRNA            complement(74..147)
                     /locus_tag="VQ514_002060"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(111..113),aa:Arg,seq:acg)
     gene            complement(157..242)
                     /locus_tag="VQ514_002061"
     tRNA            complement(157..242)
                     /locus_tag="VQ514_002061"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(206..208),aa:Leu,seq:taa)
     gene            complement(250..321)
                     /locus_tag="VQ514_002062"
     tRNA            complement(250..321)
                     /locus_tag="VQ514_002062"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Gly,seq:gcc)
     gene            complement(344..416)
                     /locus_tag="VQ514_002063"
     tRNA            complement(344..416)
                     /locus_tag="VQ514_002063"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(381..383),aa:Thr,seq:tgt)
     gene            complement(435..516)
                     /locus_tag="VQ514_002064"
     tRNA            complement(435..516)
                     /locus_tag="VQ514_002064"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(480..482),aa:Leu,seq:tag)
     gene            complement(522..594)
                     /locus_tag="VQ514_002065"
     tRNA            complement(522..594)
                     /locus_tag="VQ514_002065"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(559..561),aa:Lys,seq:ttt)
     gene            complement(619..691)
                     /locus_tag="VQ514_002066"
     tRNA            complement(619..691)
                     /locus_tag="VQ514_002066"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(656..658),aa:Asp,seq:gtc)
BASE COUNT          179 a          205 c          149 g          159 t
ORIGIN      
        1 cgaacctgcg acaccttggt cccaaaccaa gtactctacc aagctgagct acttcccgag
       61 ttaaatagaa aaatgcaccc tagaggagtc gaacctctaa ccgcctgatt cgtagtcagg
      121 tactctatcc agttgagcta agggtgctcc atattatgcc gaggaccgga atcgaaccgg
      181 tacgatcgtt accaatcgca ggattttaag tcctgtgcgt ctgccagttc cgccaccccg
      241 gcctctctaa gcgaacgacg ggattcgaac ccgcgacccc caccttggca aggtggtgtt
      301 ctaccactga actacgttcg cactgttttc ttctatctaa aaatgccggc tacatgactt
      361 gaacacgcga ccctctgatt acaaatcaga tgctctacca actgagctaa gccggctcat
      421 ttgttatatc ttaatgcggg ttaagggact tgaaccccca cgccgttaag cgccagatcc
      481 taaatctggt gcgtctgcca attccgccaa acccgcatat atgacccgta ctgggctcga
      541 accagtgacc cattgattaa aagtcaattg ctctaccaac tgagctaacg agtctaaaat
      601 aacttgcgtt accttaaacg gtcccgacgg gaatcgaacc cgcgatcttc gccgtgacag
      661 ggcgacgtga taaccgctac actacgggac ct
//
LOCUS       DAVBCW010000200          644 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.200, whole genome shotgun sequence.
ACCESSION   DAVBCW010000200 DAVBCW010000000
VERSION     DAVBCW010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.200"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(95..499)
                     /locus_tag="VQ514_002067"
     CDS             complement(95..499)
                     /locus_tag="VQ514_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HET2500767.1"
                     /translation="MKTFFAKKRNIFLARLFLGQLPLLVSTYLFLSRQFLNFSVVFQF
                     LLVVINLASILVTVYLTREMRIREFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITA
                     GESYQQIIAYIGAVLCLIIMLLLMWGLKYYKK"
BASE COUNT          245 a          117 c           99 g          183 t
ORIGIN      
        1 tatactatac catattttgt ttcgcaggga atctattata aaagggtaag tattgcaaaa
       61 acacttaccc ttttctttta tacttcatta agctctactt tttataatac ttcaagcccc
      121 acatgagcag aagcatgatg attaagcaga gaacagcgcc aatataagcg attatttgtt
      181 ggtaggattc tcctgctgtg atacctctat acaaacaaat aatagacata aaacctgtca
      241 agccgatgaa cataagttga ttggttctag gactaaccaa atcatcatct tcaaactctc
      301 ttatcctcat ttccctagtg agataaacag taaccaaaat agaagccaag ttaataacta
      361 ctaaaagaaa ttggaaaact acggaaaaat ttaaaaactg acgagataga aatagataag
      421 tagaaacaag caagggcaac tgacctaaga acaatctcgc aaggaagatg ttccgttttt
      481 tagcaaaaaa agttttcatt tcttttctcc tttcttttta ttgatagcaa aatagatcat
      541 aactgcaatc acataggcta tggtataaaa tagctgatac caagcactct ccctaagcgg
      601 atatagaaag atggacatga ttagatacag aacgaaaata atca
//
LOCUS       DAVBCW010000201          636 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.201, whole genome shotgun sequence.
ACCESSION   DAVBCW010000201 DAVBCW010000000
VERSION     DAVBCW010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.201"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            <1..>636
                     /locus_tag="VQ514_002068"
     rRNA            <1..>636
                     /locus_tag="VQ514_002068"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          179 a          135 c          181 g          141 t
ORIGIN      
        1 agcgaagttt agtagcgaag ttagtgacgt cacactgcca agaaaagctt ctagcgttta
       61 aacatactct acccgtaccg caaaccgaca caggtagtcg aggcgagtag cctcaggtga
      121 gcgagagaac tctcgttaag gaactcggca aaatgacccc gtaacttcgg gagaaggggt
      181 gctgacttta agtcagccgc agtgaatagg cccaagcaac tgtttatcaa aaacacagct
      241 ctctgctaaa tcgtaagatg atgtataggg ggtgacgcct gcccggtgct ggaaggttaa
      301 gaggagtgct tagcgtaagc gaaggtatga attgaagccc cagtaaacgg cggccgtaac
      361 tataacggtc ctaaggtagc gaaattcctt gtcgggtaag ttccgacccg cacgaaaggc
      421 gtaatgattt gggcactgtc tcaacgagag actcggtgaa attttagtac ctgtgaagat
      481 gcaggttacc cgcgacagga cggaaagacc ccatggagct ttactgcagt ttgatattga
      541 gtgtctgtac cacatgtaca ggataggtag gagtctaaga gatcgggacg ccagtttcga
      601 aggagacgct gttgggatac tacccttgtg ttatgg
//
LOCUS       DAVBCW010000202          618 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.202, whole genome shotgun sequence.
ACCESSION   DAVBCW010000202 DAVBCW010000000
VERSION     DAVBCW010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.202"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(1..227)
                     /locus_tag="VQ514_002069"
                     /pseudo
     CDS             complement(1..227)
                     /locus_tag="VQ514_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897432.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(303..530)
                     /locus_tag="VQ514_002070"
     CDS             complement(303..530)
                     /locus_tag="VQ514_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2500768.1"
                     /translation="MRERLLTQPLLHADETSYRVLESHSHLTYYWTFLSGKVENQAIT
                     LYHHDQSRSGSVVQEFLGDYSGYVHCDMLRQ"
BASE COUNT          174 a          146 c          123 g          175 t
ORIGIN      
        1 tggagatgtt cttgacgttt ctgtagtcgt tcatcagctg gcaaagcctc ccagtctctt
       61 tccaaggaaa ataactgatc acaataagct aaacctttag ctcctaagga tgatttatct
      121 gcttgcttgg gggtcgcttc aaaaaatttc cttctcacat gcgcccaaca tccaacaagt
      181 tcagcttctt ccagttgacg ataagcttct agcagttcgc agtttaccaa gcttagcgtc
      241 gccttgctcc taaaccttgg actgctatcg cataggcaga actagaggac taaagtccta
      301 agttactgcc gcaacatatc acaatgaaca tagccagaat aatctcctag gaattcttgt
      361 actaccgaac cactccgact ctgatcatga tggtacagcg tgattgcttg attctcaact
      421 ttcccagaca aaaaggtcca atagtaggtc agatggctat gactctctag aacccgataa
      481 gaagtttcat ccgcatgaag taagggctga gtcaatagtc tctctcgcaa gaggttataa
      541 aggggcttta ggaataggag ctttcacaag cttatccaga tgattatctt ttactcgtta
      601 tggacaatgc tatatggc
//
LOCUS       DAVBCW010000203          617 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.203, whole genome shotgun sequence.
ACCESSION   DAVBCW010000203 DAVBCW010000000
VERSION     DAVBCW010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.203"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            <1..>617
                     /locus_tag="VQ514_002071"
     CDS             <1..>617
                     /locus_tag="VQ514_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET2500769.1"
                     /translation="ARGVAVPHGNHYHFIPYEQMSELEKRIARIIPLRYRSNHWVPDS
                     RPEQPSPQSTPEPSPSLQPAPNPQPAPSNPIDEKLVKEAVRKVGDGYVFEENGVSRYI
                     PAKDLSAETAAGIDSKLAKQESLSHKLGAKKTDLPSSDREFYNKTYDLLARIHQDLLD
                     NKGRQVDFEALDNLLERLKDVSSDKVKLVDDILAFLAPIRHPERLG"
BASE COUNT          195 a          133 c          126 g          163 t
ORIGIN      
        1 gccagaggtg tagctgtccc tcatggtaac cattaccact ttatccctta tgaacaaatg
       61 tctgaattgg aaaaacgaat tgctcgtatt attccccttc gttatcgttc aaaccattgg
      121 gtaccagatt caagaccaga acaaccaagt ccacaatcga ctccggaacc tagtccaagt
      181 ctgcaacctg caccaaatcc tcaaccagct ccaagcaatc caattgatga gaaattggtc
      241 aaagaagctg ttcgaaaagt aggcgatggt tatgtctttg aggagaatgg agtttctcgt
      301 tatatcccag ccaaggatct ttcagcagaa acagcagcag gcattgatag caaactggcc
      361 aagcaggaaa gtttatcgca taagctagga gctaagaaaa ctgacctccc atctagtgat
      421 agagaatttt acaataagac ttatgactta ctagcaagaa ttcaccaaga tttacttgat
      481 aataaaggtc gacaagttga ttttgaggct ttggataacc tgttggaacg actcaaggat
      541 gtctcaagtg ataaagtcaa gttagtggat gatattcttg ccttcttagc tccgattcgt
      601 catccagaac gtttagg
//
LOCUS       DAVBCW010000204          616 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.204, whole genome shotgun sequence.
ACCESSION   DAVBCW010000204 DAVBCW010000000
VERSION     DAVBCW010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.204"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(194..>616)
                     /locus_tag="VQ514_002072"
     CDS             complement(194..>616)
                     /locus_tag="VQ514_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HET2500770.1"
                     /translation="KPNEEKPQTEKPEEDKEHDEVSEPTHPESDEKENHAGLNPSADN
                     LYKPSTDTEETEEEAEDTTDEAEIPQVEHSVINAKIAEAEALLEKVTDSSIRQNAVET
                     LTGLKSSLLLGTKDNNTISAEVDSLLALLKESQPTPIQ"
BASE COUNT          158 a          121 c           93 g          244 t
ORIGIN      
        1 taaatgatga agttttttct caacttcttt tactttatta aatagttttc taaaccggtt
       61 atttaaccat ttaattaacc agtaaatagt ttactattta aaattacttt tgtcaagttt
      121 ctgttacgtt tttctaaaaa agagaagtaa cacgaaaggt tacttctcca taaaatatgc
      181 tccattcatt ttactactgt ataggagtcg gttgactttc ttttaacaaa gccaagagac
      241 tatctacttc tgctgaaata gtgttattat ctttcgttcc gagaagaaga ctacttttta
      301 gaccagtcaa tgtctccaca gcattttgtc taatactaga atctgttact ttttctagca
      361 aggcctccgc ctctgctatc ttagcgttaa taacagaatg ctctacttga ggaatttcag
      421 cctcatctgt agtatcttca gcttcttcct ctgtctcttc cgtatcagtg cttggtttat
      481 aaagattatc tgctgaagga tttaaaccag cgtgattctc tttttcatca gattcagggt
      541 gagttggctc acttacttca tcatgttcct tatcttcctc aggtttttct gtctgaggtt
      601 tctcctcgtt tggttt
//
LOCUS       DAVBCW010000205          606 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.205, whole genome shotgun sequence.
ACCESSION   DAVBCW010000205 DAVBCW010000000
VERSION     DAVBCW010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.205"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            165..383
                     /locus_tag="VQ514_002073"
     CDS             165..383
                     /locus_tag="VQ514_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HET2500771.1"
                     /translation="MKKISVTVIGYFEINIDENITDILYVNGTAILYHYLRSIVSIVS
                     AIDSSEAMLLPTINVLELLDKSQPFEEE"
BASE COUNT          208 a           69 c          108 g          221 t
ORIGIN      
        1 ttttcattta gtataacaga ttgttagaag ttggtttaaa tttcctaatc agtttgttca
       61 catttacctt cgatatatta tatcccatag ttaaggttgg tcatacagat gattatagtc
      121 atggagccgt aaaacttagt gtttctttag ttgacaaaga tgccatgaaa aaaatatctg
      181 taactgtaat aggatatttt gaaataaata tagatgaaaa tatcaccgat attctatacg
      241 taaatggtac tgctattctt tatcattatt tacgttctat tgtttcaata gtttcggcaa
      301 ttgatagcag tgaagcaatg ttgctaccta ccattaatgt tttagagtta ctagataaat
      361 ctcaaccttt tgaagaagaa taatttatta gctcactaaa ttgagggtaa ggaaaagtaa
      421 aatcagtaag aaaaatgtct tgcattatac agcaaccttt tgggaatgag tagatggatt
      481 gaataaaatt ttattaagag tggatgattt atctgtagat tattattgga cagttagtct
      541 tgaagtagtc taagaattag gttataatca gtagaatcct tgctaataat gaggaggtta
      601 gtttat
//
LOCUS       DAVBCW010000206          587 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.206, whole genome shotgun sequence.
ACCESSION   DAVBCW010000206 DAVBCW010000000
VERSION     DAVBCW010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.206"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            <1..>587
                     /locus_tag="VQ514_002074"
     rRNA            <1..>587
                     /locus_tag="VQ514_002074"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          144 a          134 c          183 g          126 t
ORIGIN      
        1 gacgggcagt ttgactgggg cggtcgcctc ctaaaaggta acggaggcgc ccaaaggttc
       61 cctcagaatg gttggaaatc attcgcagag tgtaaaggta taagggagct tgactgcgag
      121 agctacaact cgagcaggga cgaaagtcgg gcttagtgat ccggtggttc cgtatggaag
      181 ggccatcgct caacggataa aagctaccct ggggataaca ggcttatctc ccccaagagt
      241 tcacatcgac ggggaggttt ggcacctcga tgtcggctcg tcgcatcctg gggctgtagt
      301 cggtcccaag ggttgggctg ttcgcccatt aaagcggcac gcgagctggg ttcagaacgt
      361 cgtgagacag ttcggtccct atccgtcgcg ggcgtaggaa atttgagagg atctgctcct
      421 agtacgagag gaccagagtg gacttaccgc tggtgtacca gttgtcttgc caaaggcatc
      481 gctgggtagc tatgtaggga agggataaac gctgaaagca tctaagtgtg aaacccacct
      541 caagatgaga tttcccatga ttatatatca gtaagagccc tgagaga
//
LOCUS       DAVBCW010000207          573 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.207, whole genome shotgun sequence.
ACCESSION   DAVBCW010000207 DAVBCW010000000
VERSION     DAVBCW010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.207"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(<1..>573)
                     /locus_tag="VQ514_002075"
     CDS             complement(<1..>573)
                     /locus_tag="VQ514_002075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500772.1"
                     /translation="DAEAEALVDAEADALVLAEADALVLAEAEALVDAEAEALVLAEA
                     EALVDAEADALVEADSEAEVLAEAEALVDAEADALVLAEAEALVDADSEADVLAEAEA
                     LVLAEVEALVEADSDALVEADSDAEVLAEAEALVDAEAEALVLAEADALVLAEAEALV
                     LAEAEALVLAEAEALVDAEAEALVDADSDA"
BASE COUNT          139 a          195 c          127 g          112 t
ORIGIN      
        1 cagcgtctga atcggcatca acgagcgcct cagcttcagc gtcaaccagt gcttcggctt
       61 cagcaagcac cagcgcctcg gcttcagcaa gtactagcgc ctcagcttca gcaagtacca
      121 gtgcgtcagc ctcagcaagt accagtgctt cagcctcagc gtcgacaagt gcctcggctt
      181 cagcaagtac ctcagcgtct gaatcggcct caaccagtgc atctgaatcg gcctcaacca
      241 gtgcctcaac ctcagcaagt accagtgctt cagcctcagc aagcacatca gcttctgaat
      301 cagcatcaac cagtgcctca gcctcagcaa gtactagtgc atcagcttca gcatcaacga
      361 gtgcttcagc ttcagcaagc acctcagctt ctgaatcggc ctcaacgagt gcgtcagcct
      421 cagcgtcaac gagtgcttcg gcttcagcaa gcacaagcgc ctcagcttcc gcatcaacaa
      481 gtgcctcggc ttcagcaagt accagtgcgt cagcttcagc aagcacaagt gcgtcagctt
      541 cagcgtcaac cagcgcctca gcttcagcat caa
//
LOCUS       DAVBCW010000208          560 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.208, whole genome shotgun sequence.
ACCESSION   DAVBCW010000208 DAVBCW010000000
VERSION     DAVBCW010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.208"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            <1..>560
                     /locus_tag="VQ514_002076"
     CDS             <1..>560
                     /locus_tag="VQ514_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET2500773.1"
                     /translation="YTDDEIQVAKLAGKYTTEDGYIFDPRDITSDEGDAYVTPHMTHS
                     HWIKKDSLSEAERAAAQAYAKEKGLTPPSTDHQDSGNTEVKGAEAIYNRVKAAKKVPL
                     DRMPYNLQYTVEVKNGSLIIPHYDHYHNIKFEWFDEGLYEAPKGYTLEDLLATVKYYV
                     EHPNERPHSDNGFGNASDHVQRNKNG"
BASE COUNT          183 a          113 c          126 g          138 t
ORIGIN      
        1 tacactgatg atgagattca agtagccaag ttggcaggca agtacacaac agaagacggt
       61 tatatctttg atcctcgtga tataaccagt gatgaggggg atgcctatgt aactccacat
      121 atgacccata gccactggat taaaaaagat agtttgtctg aagctgagag agcggcagcc
      181 caggcttatg ctaaagagaa aggtttgacc cctccttcga cagaccatca ggattcagga
      241 aatactgagg taaaaggagc agaagctatc tacaaccgcg tgaaagcagc taagaaggtg
      301 ccacttgatc gtatgcctta caatcttcaa tatactgtag aagtcaaaaa cggtagttta
      361 atcatacctc attatgacca ttaccataac atcaaatttg agtggtttga cgaaggcctt
      421 tatgaggcac ctaaggggta tactcttgag gatcttttgg cgactgtcaa gtactatgtc
      481 gaacatccaa acgaacgtcc gcattcagat aatggttttg gtaacgctag cgaccatgtt
      541 caaagaaaca aaaatggtca
//
LOCUS       DAVBCW010000209          547 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.209, whole genome shotgun sequence.
ACCESSION   DAVBCW010000209 DAVBCW010000000
VERSION     DAVBCW010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.209"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(259..346)
                     /locus_tag="VQ514_002077"
     tRNA            complement(259..346)
                     /locus_tag="VQ514_002077"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(310..312),aa:Ser,seq:gct)
     gene            complement(353..426)
                     /locus_tag="VQ514_002078"
     tRNA            complement(353..426)
                     /locus_tag="VQ514_002078"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(390..392),aa:Ile,seq:gat)
     gene            complement(462..532)
                     /locus_tag="VQ514_002079"
     tRNA            complement(462..532)
                     /locus_tag="VQ514_002079"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(498..500),aa:Gly,seq:tcc)
BASE COUNT          184 a          124 c           93 g          146 t
ORIGIN      
        1 atctcatcaa acttatcaag tgcaatgcct ttggttttcg aaactttgaa aactttaaaa
       61 aacggatttt tatcgctttg aacatcaaaa aagaaaggac gaaatttgtc ctttctcgag
      121 cttagctttt cttcaaccca ctacagttga caaagagcca taaaaataac ttcagattag
      181 taattcaatt aagttttacg gatctgaagc tttattgtct ctaaaaataa aaaagagagt
      241 tacaaactct cattaaaacg gagaataagg gattcgaacc cttgcgccag ttacccgacc
      301 taacgattta gcaaaccgtc ctcttcagcc tcttgagtaa ttctccaatt aatgggcacg
      361 agtggactcg aaccaccgac ctcacgctta tcaggcgtgc gctctaacca cctgagctac
      421 gcgcccaagt taaaaaactt ggtaatttga acaaagttca aagcgggtga cgagaatcga
      481 actcgcgaca acagcttgga aggctgtagt tttaccacta aactacaccc gcataaatac
      541 tatcaat
//
LOCUS       DAVBCW010000210          531 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.210, whole genome shotgun sequence.
ACCESSION   DAVBCW010000210 DAVBCW010000000
VERSION     DAVBCW010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.210"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
BASE COUNT          135 a          128 c           95 g          173 t
ORIGIN      
        1 tgacttgcat cagtagcggc cttattgaca tttttcagcg ccttctgcaa gggttgcgtg
       61 tcgccatcga tttcaatttt gatacctttg atatttcctg ccatatttcc tcctttctca
      121 aaaaaataga aaagcgctga gagaacttct accactgata atgcagccag accaaggaac
      181 ttggtctcag aatcgctctc tcagcactca tttttcttta aaaactgtca aaatcagctt
      241 gcgtggcttt ccgttcgcca cccttatcct cgctccgtag attcacataa tccgtctgat
      301 aatccagagc cattccgatt gaaatgtgct ttagatcatc aatagaaaga ccagtttctt
      361 tacagcaaga tagataggat tctactgtga agatttcttc gctagctgat tctgactcat
      421 ctggtgcttt tttgtcgtca tgctcgcatt tagcatttcc atcagcacag gtccaacttc
      481 ctgaatcggg aagatttcca tttccatgaa gaattgttca taaggcttga t
//
LOCUS       DAVBCW010000211          513 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.211, whole genome shotgun sequence.
ACCESSION   DAVBCW010000211 DAVBCW010000000
VERSION     DAVBCW010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.211"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(84..513)
                     /locus_tag="VQ514_002080"
                     /pseudo
     CDS             complement(84..513)
                     /locus_tag="VQ514_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547411.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          130 a          107 c           93 g          183 t
ORIGIN      
        1 ctaggataaa gcaaagagcc tgggacaaaa tagttctcga ccacaaaaaa gctagagatt
       61 tccaattgtg gaactttagc tttttaattt tgagtcgttc tcttattgta ttttaggagg
      121 ttattggcca taagtaccaa tcccatgtca attctcactt gacgcttgcc cctcagatta
      181 catctcttgt aacccaaaca agcctttatc tgcccaaaga caggtgacat tctttagctt
      241 tcaagttttg atagccttcg ttcatataca gtcccttttg aggggctgat tcaggttcgt
      301 cggcgtagta aaccttgatt tcttgtttaa agtctgtctg tgttttctga tgtttggtat
      361 ggtgaaaacg ataataccag ccatcaggat gtgtatagct atcctccttg tcattatagt
      421 accaattcgc taagtttcta gctgactgtt tatagcctct cttctgttcc ttatcaaaca
      481 tggcatattt aatcagatgg tttacctcct ttt
//
LOCUS       DAVBCW010000212          513 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.212, whole genome shotgun sequence.
ACCESSION   DAVBCW010000212 DAVBCW010000000
VERSION     DAVBCW010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.212"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            152..460
                     /locus_tag="VQ514_002081"
                     /pseudo
     CDS             152..460
                     /locus_tag="VQ514_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
BASE COUNT          204 a           64 c           97 g          148 t
ORIGIN      
        1 tatcgcttac caatatttgc taaactttca gaaaaaaagt tggagtatat gatgctaggt
       61 gactgcgtca tgttagtaaa cgaaatggaa ataactgatc atagagtaga taacttattt
      121 gaaaaaggga aaaatgagat aaaagattca agtagtagat ttagcaatag gacatataaa
      181 agctttagaa aaagtatctg aaaaaacaga tgtttatatc tataacctag gctcgggaga
      241 aggcactagt gtattacaac ttgtaaatac atttgaaagt gttaataaga tccctattcc
      301 ttataaaata gttccaagac gttcaggaga cgttgcaact tattatgcaa atgcagacaa
      361 agcatataag gaattaaatt ggaggacaac aaaatcgatt gaagacatgt gtagagatac
      421 atggaattgg caatcaaaaa atcccaatgg ctataattga tgatactgtc tatatgagtg
      481 ctgtattttg gaaaaattac ttaacctttt aat
//
LOCUS       DAVBCW010000213          501 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.213, whole genome shotgun sequence.
ACCESSION   DAVBCW010000213 DAVBCW010000000
VERSION     DAVBCW010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.213"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            <1..>501
                     /locus_tag="VQ514_002082"
     CDS             <1..>501
                     /locus_tag="VQ514_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2500774.1"
                     /translation="FGENKIFESIDNLFDIIDGDRGKNYPKSDELFSEEYCLFLNTKN
                     VTKNGFSFDTKQFITKTKDKLLRKGKLERYDIVLTTRGTVGNVAYYDELIKYKHLRIN
                     SGMVILRPKTPNLNQKFIIHVLRNNNYSRVISGSAQPQLPITKLKKILLPLPPLALQN
                     EFADFVA"
BASE COUNT          190 a           76 c           84 g          151 t
ORIGIN      
        1 gtttggggaa aataaaatat ttgaaagcat tgataactta tttgatatta tagatggtga
       61 taggggcaaa aattatccta aatcagatga gttgtttagt gaggagtact gtttattttt
      121 aaatacaaag aatgttacta aaaacggatt ttcattcgat acaaagcaat ttatcactaa
      181 aacaaaggat aaattacttc gaaaaggcaa gcttgagcgt tatgatatag tcttgacaac
      241 aagaggtact gttggaaatg tagcgtacta cgatgaatta ataaaatata aacatttacg
      301 tataaattca ggtatggtaa tattacgtcc caagacacca aatctaaatc agaaatttat
      361 tatccatgtt ttaaggaata ataattatag tcgagtgata tcaggaagtg ctcagcctca
      421 gttaccaatt acaaaattaa aaaaaatact tctccccctc cccccactag ccctccaaaa
      481 tgagttcgca gactttgtag c
//
LOCUS       DAVBCW010000214          500 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.214, whole genome shotgun sequence.
ACCESSION   DAVBCW010000214 DAVBCW010000000
VERSION     DAVBCW010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.214"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(4..381)
                     /locus_tag="VQ514_002083"
     CDS             complement(4..381)
                     /locus_tag="VQ514_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HET2500775.1"
                     /translation="MICQFIRDMLDLPAKNVTILEGSNIHVLPSMPYSAQDFYTSIDV
                     LAELDNGIQVIIEIQVHHQNFFINRLWAYLCSQVNQNLEKIRQREGDTHQSYKQIALV
                     YAIAIVDSNYFSDDLAFHSFIVK"
BASE COUNT          143 a           94 c           97 g          166 t
ORIGIN      
        1 atttcatttt actataaaac tatgaaaagc taggtcatct gagaagtaat tactatcgac
       61 aattgcgata gcgtatacta gtgcgatttg tttgtagctc tggtgggtat caccttcacg
      121 ttggcgaatt ttttctaggt tttgattaac ctgactgcac agataagccc ataggcgatt
      181 gatgaaaaaa ttctgatgat gaacctgaat ttcgatgata acttggattc cattatctaa
      241 ctccgccaag acgtctatac tagtatagaa atcttgcgct gagtagggca tggaaggcaa
      301 gacgtgaatg ttacttccct ccaaaatcgt cacatttttt gctggcaagt ccagcatatc
      361 gcggataaat tgacaaatca ttagttgggc tgatacctgg atgtctgaga atcctccttt
      421 tcctccttct attatagcac atttttaaat ctaggtttgc tagattctat gcttctatct
      481 atttattcgg aaaagaaagc
//
LOCUS       DAVBCW010000215          486 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.215, whole genome shotgun sequence.
ACCESSION   DAVBCW010000215 DAVBCW010000000
VERSION     DAVBCW010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.215"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(126..>486)
                     /locus_tag="VQ514_002084"
     CDS             complement(126..>486)
                     /locus_tag="VQ514_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2500776.1"
                     /translation="YDEIMAMPMKFNTIISEMGSNISGGQRQQIALARALINNPSIVI
                     LDEATSALDTINEERITKYIQSQGCTQIIIAHRLSTIKDADIIFVMKGGKIVESGNHK
                     YLITLGGEYYSLYTKRK"
BASE COUNT          149 a          104 c           54 g          179 t
ORIGIN      
        1 cctaaagaaa taccatattc aactggaaaa tctaaaaaaa ttgcaaacac tattccaatc
       61 atcaccccta gtgaggctat tgttaaaatt acatattcat tttctttctt cattttcttt
      121 acacctcatt tcctttttgt atataagctg tagtactctc caccaagagt tattaagtac
      181 ttatgatttc ctgactcaac aatcttacca cctttcatta caaaaataat atccgcatcc
      241 ttaatcgttg acaatctatg agctataatt atttgagtac aaccctgact ttgtatatac
      301 tttgttattc tttcctcatt aatagtgtct aatgcactag ttgcttcatc taaaattaca
      361 atactaggat tatttattaa tgcacgtgcc agtgctatct gttgcctttg cccacctgaa
      421 atatttgacc ccatctctga gatgatagta ttaaatttca tcggcatagc catgatttca
      481 tcatag
//
LOCUS       DAVBCW010000216          486 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.216, whole genome shotgun sequence.
ACCESSION   DAVBCW010000216 DAVBCW010000000
VERSION     DAVBCW010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.216"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(55..128)
                     /locus_tag="VQ514_002085"
     tRNA            complement(55..128)
                     /locus_tag="VQ514_002085"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(92..94),aa:Met,seq:cat)
     gene            complement(142..231)
                     /locus_tag="VQ514_002086"
     tRNA            complement(142..231)
                     /locus_tag="VQ514_002086"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(193..195),aa:Ser,seq:tga)
     gene            complement(314..387)
                     /locus_tag="VQ514_002087"
     tRNA            complement(314..387)
                     /locus_tag="VQ514_002087"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(351..353),aa:Ile,seq:gat)
BASE COUNT          137 a          134 c          109 g          106 t
ORIGIN      
        1 gccgacacat ggagcttcaa tccattgctc taccaactga gctaccgagc cttattgcgg
       61 gagcaggatt tgaacctacg accttcgggt tatgagcccg acgagctacc gagctgctcc
      121 atcccgcgtt aataatataa aaggaggatg tgggattcga acccacgcac gcttttacac
      181 gcctgacggt tttcaagacc gttcccttca gccggacttg ggtaatcctc caaaatagtt
      241 tatacgggaa caagcccgtg tcctcttagt gaacagataa tattccaatc ccctagtaat
      301 ggactaaaca ctatgggcac gagtggactc gaaccaccga cctcacgctt atcaggcgtg
      361 cgctctaacc acctgagcta cgcgcccaag tcaaaaactt ggtacaaaga acaaagttca
      421 aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtagt tttaccacta
      481 aactac
//
LOCUS       DAVBCW010000217          482 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.217, whole genome shotgun sequence.
ACCESSION   DAVBCW010000217 DAVBCW010000000
VERSION     DAVBCW010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.217"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            30..>482
                     /locus_tag="VQ514_002088"
     CDS             30..>482
                     /locus_tag="VQ514_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET2500777.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVF"
BASE COUNT          178 a           62 c          116 g          126 t
ORIGIN      
        1 gttagactgt atctagaaag gggaaaatta tgattaaaga attgtatgaa gaagtccaag
       61 ggactgtgta taagtgtaga aatgaatatt accttcattt atgggaattg tcggattggg
      121 accaagaagg catgctctgc ttacatgaat tgattagtag agaagaagga ctggtagacg
      181 atattccacg tttaaggaaa tatttcaaaa ccaagtttcg aaatcgaatt ttagactata
      241 tccgtaagca ggaaagtcag aagcgtagat acgataaaga accctatgaa gaagtgggtg
      301 agatcagtca tcgtataagt gaggggggtc tctggctaga tgattattat ctctttcatg
      361 aaacactaag agattataga aacaaacaaa gtaaagagaa acaagaagaa ctagaacgcg
      421 tcttaagcaa tgaacgattt cgagggcgtc aaagagtatt aagagactta cgcattgtgt
      481 tt
//
LOCUS       DAVBCW010000218          463 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.218, whole genome shotgun sequence.
ACCESSION   DAVBCW010000218 DAVBCW010000000
VERSION     DAVBCW010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.218"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(137..>463)
                     /locus_tag="VQ514_002089"
     CDS             complement(137..>463)
                     /locus_tag="VQ514_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HET2500778.1"
                     /translation="KPQTEKPEEETPREEKPQSEKPESPKPTEEPEEESPEESEEPQV
                     ETEKVEEKMREAEDLLGKIQDPIIKSNAKETLTGLKNNLLFGTQDNNTIMAEAEKLLA
                     LLKESK"
BASE COUNT           90 a           98 c           77 g          198 t
ORIGIN      
        1 tactattcta gtttcaatct actatatttt gcacattttc atgaaaaaaa tgagaactag
       61 aactcacatt ctgctctcat ttttcgtttt cccgttctcc tatcctgttt ttaggagtta
      121 gaaaatgctg ctacctttac ttactctcct ttaataaagc caatagtttt tcagcttctg
      181 ccataatagt attgttgtcc tgggtgccaa atagtaaatt attttttaat cctgtgagag
      241 tctctttggc attggacttg ataattggat cctggatttt tccaagtaaa tcttcagcct
      301 ctctcatttt ttcttcaacc ttttcagtct cgacctgagg ttcttctgat tcctctggtg
      361 attcttcttc tggttcctct gttggttttg gagactctgg tttctcgctt tgcggtttct
      421 cttctcgagg ggtttcttcc tcaggttttt ctgtctgagg ttt
//
LOCUS       DAVBCW010000219          449 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.219, whole genome shotgun sequence.
ACCESSION   DAVBCW010000219 DAVBCW010000000
VERSION     DAVBCW010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.219"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
BASE COUNT          145 a           77 c          109 g          118 t
ORIGIN      
        1 cggataagga ggcttcaaac tctaaagtcc aaaaaggtag tcgtaaccta tatgtgtaaa
       61 tcacgagagt aattgaattc ggactaaggt ttgtgtgaaa aagataaatc tttctagagt
      121 ctaaagactc tgcgtcagat ttcctatttt cactgtaacc ttttaacgtc ctcatatctt
      181 gtataaacga ggaaagatgt acgacttatc ccgtgaggtt tcatgagcgc tgaaagcgta
      241 gtaacaacga atcatgagaa gtcagccgag cccatagtag tgaggaaact tccgtaatgg
      301 aagtggagcg aaggggtgaa tactcaaaca gtctggggag agactgtttg aggtctgtcg
      361 ctagaaagag aaaacgacag atcgaagtaa tcctacttca cttgtgtctg taaaatgagt
      421 ggtctgatag aactggactt tgacacgta
//
LOCUS       DAVBCW010000220          432 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.220, whole genome shotgun sequence.
ACCESSION   DAVBCW010000220 DAVBCW010000000
VERSION     DAVBCW010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.220"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(1..432)
                     /locus_tag="VQ514_002090"
                     /pseudo
     CDS             complement(1..432)
                     /locus_tag="VQ514_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          136 a           78 c           95 g          123 t
ORIGIN      
        1 gatagtggtg tttcaagtct tccgaatagc tcaaaagctt gtctaaaatc tctttattgg
       61 ttaaatgcat acaaaaagta ggacgataaa aacgcttatc actcagttta cggctatcct
      121 gttgtatgag cttccagtag cgcttgatag ccttgtattc atgggatttt cgatgaaact
      181 gattcatgat ttaaacacgc acacgactca tagcacggct aagatgttgc acaatgtgaa
      241 agcgatcaag aacgatttta gcattcggga gtgaaacagt ctgggagact gtttcagcct
      301 gagcctagaa atttgaaagc gaagctgtct agctaagtca tagtaagggc taaacatatc
      361 catagtaata attttgacgc gacatcggac aactctatcg tagcgaagaa agtgatttcg
      421 aatgatagct tg
//
LOCUS       DAVBCW010000221          432 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.221, whole genome shotgun sequence.
ACCESSION   DAVBCW010000221 DAVBCW010000000
VERSION     DAVBCW010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.221"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
BASE COUNT          143 a           82 c           75 g          132 t
ORIGIN      
        1 tggtttcgca ggaagtctat tatatatgca cagacatatg aaatcttttt gagacagcag
       61 ttatgatata tttatccatt cacaacatct tctgtactaa tactacaata ttggtacatt
      121 ttgattacac aatcgtttca aaaaatatga tggaagcctg agtattttat agtaattccc
      181 ttcctatcaa ctagatcaac cagttgagtg ttgaatgttg aaacacccta gcctcagcaa
      241 aggactatga gcagcatttc ctagccaata ggacgctcca aatcacctat tctaaatcca
      301 tcactagctt ggtcagtaag atacagactg caaggcgaga ctgacgtgtt tgatgagatt
      361 ttggaagagt attacaatca tagattcacc agtagacttg accgcagatt taactatagt
      421 agattgaaac ta
//
LOCUS       DAVBCW010000222          422 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.222, whole genome shotgun sequence.
ACCESSION   DAVBCW010000222 DAVBCW010000000
VERSION     DAVBCW010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.222"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(<1..>422)
                     /locus_tag="VQ514_002091"
     CDS             complement(<1..>422)
                     /locus_tag="VQ514_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2500779.1"
                     /translation="TVLSRLGDLSLPCPQDEDLTILPHSAYSETLYQAGSWSHKRRVC
                     QFSERKEGNLFYDVISLVTNMTSGTSQDQFQLYRGRGQAENFIKEMKEGFFGDKTDSS
                     TLIKNEVRMMMSCIAYNLYLFLKHLAGGDFQTLTIKRFR"
BASE COUNT          116 a           87 c          102 g          117 t
ORIGIN      
        1 cggaagcgtt tgattgttaa agtttggaag tcacctccag ctagatgttt gagaaaaaga
       61 tagagattgt aggcgataca gctcatcatc atacgaactt cgtttttgat taaggttgaa
      121 ctatccgttt tatcgccaaa aaatccctcc ttcatctcct tgatgaaatt ctcggcttga
      181 ccacgtccac gataaagctg aaactggtct tggcttgttc cactcgtcat atttgtaacg
      241 agagaaataa catcgtagaa caagtttcct tcttttcgtt cagagaactg gcagacacga
      301 cgcttgtgcg accaagatcc tgcttgatag agagtttctg agtaggcgga gtggggcaag
      361 atggttaagt cctcatcctg tgggcaaggg agggaaaggt ctccaagacg gctcagaaca
      421 gt
//
LOCUS       DAVBCW010000223          411 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.223, whole genome shotgun sequence.
ACCESSION   DAVBCW010000223 DAVBCW010000000
VERSION     DAVBCW010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.223"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            <1..>411
                     /locus_tag="VQ514_002092"
     CDS             <1..>411
                     /locus_tag="VQ514_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5-like element IS1515 family transposase"
                     /protein_id="HET2500780.1"
                     /translation="GKKKRHTLKTQIMLDLTTHKVCQMAFSDGHTHDFTLFKESIGQS
                     LPETTLAFVDLGYLGILKFHENTFIPAKNSKNRRLSEDDKQLNKEMSAIRIEIEHFNA
                     KFKTFQIMSVPYRNRRKRFELRAELICAIINYEV"
BASE COUNT          138 a           79 c           76 g          118 t
ORIGIN      
        1 ctggtaaaaa gaaacgacac accttaaaaa ctcaaattat gctggatttg acgacacata
       61 aagtctgtca aatggccttt tctgacggac atacgcatga ttttactctc ttcaaagaaa
      121 gtattggaca aagtttgcct gaaacgacgc ttgcctttgt tgacctaggt tatttaggca
      181 tcttgaaatt tcatgagaat actttcattc ctgctaaaaa ttccaaaaat cgccgcctga
      241 gtgaggatga taagcagtta aataaagaga tgtcagcgat acgaattgaa attgaacatt
      301 ttaacgctaa attcaagacc ttccaaatca tgtcagtccc ttatcgtaac cgcagaaaac
      361 gtttcgagtt acgggcggaa ttaatttgtg ccatcatcaa ttatgaagtg a
//
LOCUS       DAVBCW010000224          381 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.224, whole genome shotgun sequence.
ACCESSION   DAVBCW010000224 DAVBCW010000000
VERSION     DAVBCW010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.224"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            <1..>381
                     /locus_tag="VQ514_002093"
     CDS             <1..>381
                     /locus_tag="VQ514_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HET2500781.1"
                     /translation="GKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSF
                     RSQHPHYLELQQEYGKDSVEYTKDFAGKMVESLVTKLSSLRYNLLIEGTLRTVDVPKK
                     TAQLLKNKGYEVQLALIATKPKLS"
BASE COUNT          140 a           58 c           83 g          100 t
ORIGIN      
        1 gaggaaaaaa gtccagtaag caacctatag cgattttgct cggagggcaa agtggtgccg
       61 gtaagactac aattcatcgt attaaacaga aagaatttca aggaaatatt gttatcatag
      121 atggtgatag ttttcgttct cagcatccac actatttaga actgcagcaa gaatatggca
      181 aagacagtgt agaatatacc aaagattttg caggaaaaat ggtagagtct ttagtaacaa
      241 aattgagtag tttgagatac aatcttttga tagagggaac tttacgaaca gttgatgttc
      301 caaagaaaac ggcacaactc ttgaaaaata agggatatga agtacaatta gccttgattg
      361 cgacaaagcc taagctgtcc t
//
LOCUS       DAVBCW010000225          372 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.225, whole genome shotgun sequence.
ACCESSION   DAVBCW010000225 DAVBCW010000000
VERSION     DAVBCW010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.225"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            21..>372
                     /locus_tag="VQ514_002094"
     CDS             21..>372
                     /locus_tag="VQ514_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS1515 family transposase"
                     /protein_id="HET2500782.1"
                     /translation="METVYDKAQKLNSKNFKLLIGVKKETFQLMLEHLNSAYQIQHRK
                     GGRPRSLPMEDQLIMTLRYLRYYPTQRLLAFDFGVGVATVNAIITWVEDTLRASGSFD
                     LDHLEAPSAAVAIDV"
BASE COUNT          106 a           84 c           80 g          102 t
ORIGIN      
        1 aaaagtgtta tactattttt atggaaacag tatacgacaa agcacaaaaa cttaactcaa
       61 aaaacttcaa actattgatt ggtgtcaaaa aggaaacctt tcaactcatg ctagaacacc
      121 tgaattcagc ctatcagatt cagcaccgaa aaggtggacg tccacgtagt ctgcccatgg
      181 aagaccagct cattatgacc ctccgttact tgcgatatta tcccactcag cgtctgctgg
      241 cctttgattt tggcgtcggt gtagctacgg taaatgccat catcacttgg gtggaggata
      301 cacttcgtgc gtcaggtagc tttgatttgg accatttaga agccccgagt gctgctgtgg
      361 ctattgacgt ga
//
LOCUS       DAVBCW010000226          361 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.226, whole genome shotgun sequence.
ACCESSION   DAVBCW010000226 DAVBCW010000000
VERSION     DAVBCW010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.226"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            18..266
                     /locus_tag="VQ514_002095"
     CDS             18..266
                     /locus_tag="VQ514_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HET2500783.1"
                     /translation="MVCSILASTSVLAVWVDGGQWNYGVGWSGNFGYSDYLHSTRSHT
                     ATVKDGNKFSKDRAEAEAWARASIFKFPPTGMEYFYGF"
BASE COUNT           93 a           61 c           81 g          126 t
ORIGIN      
        1 ttggtgttgc atctttaatg gtatgtagta tacttgcttc tacaagtgtt ttggcagtat
       61 gggttgatgg tggtcaatgg aactatggag taggatggtc aggaaatttt ggttattctg
      121 attatttaca ttctactcgc tctcatacag caactgttaa agatggaaat aaattctcta
      181 aggatcgtgc agaagctgag gcatgggctc gggcttcaat cttcaaattc ccaccaacgg
      241 gaatggaata tttctatgga ttttaattaa aattctccct ccccttttgg agagggagtt
      301 tcttcacggg aggtatctct atgaaaaaaa tcagtcatct ttgtatgtta ctcctgcttc
      361 t
//
LOCUS       DAVBCW010000227          355 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.227, whole genome shotgun sequence.
ACCESSION   DAVBCW010000227 DAVBCW010000000
VERSION     DAVBCW010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.227"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            <1..335
                     /locus_tag="VQ514_002096"
     CDS             <1..335
                     /locus_tag="VQ514_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HET2500784.1"
                     /translation="AKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRT
                     GLDDKLLDTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEA
                     VVKLIKKV"
BASE COUNT          115 a           58 c           74 g          108 t
ORIGIN      
        1 atgccaagcg aaatgctaaa agaatgggat ttgacaatac tcattatgaa gctggaacgg
       61 cagaagagat tattcctcgt tggtacaagg aaggctaccg agcagatgct ttgattgttg
      121 acccaccacg tacaggtctg gatgataagt tattagatac tattcttact tatgtaccag
      181 aaaaaatggt ttatatttct tgtaatgttt cgaccttggc tcgtgatttg gtacgcttag
      241 tagaagtcta tgatcttcat tatatccagt cggtcgatat gttcccacat acagctcgaa
      301 ctgaagctgt tgtaaaatta ataaaaaaag tttaaaaaag gtgttgacaa aacat
//
LOCUS       DAVBCW010000228          349 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.228, whole genome shotgun sequence.
ACCESSION   DAVBCW010000228 DAVBCW010000000
VERSION     DAVBCW010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.228"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            <1..347
                     /locus_tag="VQ514_002097"
     CDS             <1..347
                     /locus_tag="VQ514_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HET2500785.1"
                     /translation="KKLREQQATMLEDIDYLKSEQPIHPSYAKSLLKKRKARVVVCLG
                     GIDSPAYADKTFAQSVFRQAEIDFKDHFNINRYDLLPKKFADAALAYWMTWEPSTNTK
                     MKIMKLNSFDEG"
BASE COUNT          108 a           72 c           84 g           85 t
ORIGIN      
        1 agaagaaatt acgtgagcaa caggcgacca tgctggaaga tattgactat ctcaagagtg
       61 agcaaccgat tcacccaagc tatgctaaat cgctcctgaa gaagcgtaag gctcgggtcg
      121 tggtttgctt aggtggcatt gatagtccgg cttatgcgga taagactttc gctcagtcag
      181 tctttagaca agctgagatt gactttaaag accacttcaa cattaatcgc tatgacttgc
      241 taccgaaaaa attcgcagat gcagccttgg cctattggat gacttgggag ccaagcacca
      301 ataccaagat gaaaatcatg aaattgaact catttgacga agggtagga
//
LOCUS       DAVBCW010000229          347 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.229, whole genome shotgun sequence.
ACCESSION   DAVBCW010000229 DAVBCW010000000
VERSION     DAVBCW010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.229"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            141..>347
                     /locus_tag="VQ514_002098"
     CDS             141..>347
                     /locus_tag="VQ514_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HET2500786.1"
                     /translation="MAIADKVIIEDFTAYVELFSSKSGQKVEEKYLHAESDYQKVLVY
                     LKNKFDYQKSIVPYVIYFLFKTGMR"
BASE COUNT          117 a           38 c           73 g          119 t
ORIGIN      
        1 tcatcgaaag gctatttgga aataagcctg tatcacaaat taagcctagc gagtatcaaa
       61 aaattatgaa cgagtatgga aaaactgtat cgagaaattt tttaggaagg ttgaattctt
      121 gtattcgggc aagtatacag atggctattg ctgataaggt gataatagag gattttactg
      181 cttatgttga gttgttttct tctaagagtg gacaaaaggt tgaggaaaag tatttgcatg
      241 ctgagtcaga ttatcaaaaa gttttagtat atttgaaaaa taagtttgat tatcagaaat
      301 ctattgtacc gtatgtaatt tatttccttt ttaaaactgg catgcgt
//
LOCUS       DAVBCW010000230          344 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.230, whole genome shotgun sequence.
ACCESSION   DAVBCW010000230 DAVBCW010000000
VERSION     DAVBCW010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.230"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            <1..>344
                     /locus_tag="VQ514_002099"
     CDS             <1..>344
                     /locus_tag="VQ514_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HET2500787.1"
                     /translation="QQQPPKTEKPAQPSTPKTGWKQENGMWYFYNTDGSMATGWLQNN
                     GSWYYLNSNGAMATGWLQNNGSWYYLNANGSMATGWLQNNGSWYYLNANGSMATGWLQ
                     YNGSWYYLNANG"
BASE COUNT          119 a           81 c           74 g           70 t
ORIGIN      
        1 caacagcaac cgccaaaaac tgaaaaacca gcacaaccat ctactccaaa aacaggctgg
       61 aaacaagaaa acggtatgtg gtacttctac aatactgatg gttcaatggc gacaggatgg
      121 ctccaaaaca atggctcatg gtactacctc aacagcaatg gcgctatggc gacaggatgg
      181 ctccaaaaca atggttcatg gtactatcta aacgctaatg gttcaatggc aacaggatgg
      241 ctccaaaaca atggctcatg gtactaccta aacgctaatg gttcaatggc gacaggatgg
      301 ctccaataca atggttcatg gtactaccta aacgctaatg gtga
//
LOCUS       DAVBCW010000231          322 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.231, whole genome shotgun sequence.
ACCESSION   DAVBCW010000231 DAVBCW010000000
VERSION     DAVBCW010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.231"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(<1..>322)
                     /locus_tag="VQ514_002100"
     CDS             complement(<1..>322)
                     /locus_tag="VQ514_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2500788.1"
                     /translation="KSHEYKAIKRYWKLIQQDSRKLSDKRFYRPTFRMHLTNKEILDK
                     ILSYSEDLKHHYQIYQLLLFHFQNKDPEKFFGLIEDNLKQVHPLFQTVFKTFLKNKEK
                     IVNAL"
BASE COUNT           94 a           46 c           70 g          112 t
ORIGIN      
        1 aagggcgttg acgattttct ctttgttctt tagaaaggtt ttaaagacag tctgaaaaag
       61 aggatgaacc tgcttcagat tgtcctcaat gagtccgaaa aatttctcag ggtctttgtt
      121 ctgaaagtga aaaagtaaga gttgatagat ctgatagtgg tgtttcaagt cttctgaata
      181 gcttaaaatc ttgtcaagaa tttctttatt tgttaagtgc atgcgaaaag tagggcgata
      241 aaaacgttta tcgctcaatt tacgactatc ctgttggatg agtttccagt aacgcttgat
      301 agccttgtat tcatgagatt tt
//
LOCUS       DAVBCW010000232          292 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.232, whole genome shotgun sequence.
ACCESSION   DAVBCW010000232 DAVBCW010000000
VERSION     DAVBCW010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.232"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            51..>292
                     /locus_tag="VQ514_002101"
     rRNA            51..>292
                     /locus_tag="VQ514_002101"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           90 a           54 c           81 g           67 t
ORIGIN      
        1 gaaaaaaaca ataaatctgt cagtgacaga aatgagtgag aactcaaact tttaatgaga
       61 gtttgatcct ggctcaggac gaacgctggc ggcgtgccta atacatgcaa gtagaacgct
      121 gaaggaggag cttgcttctc tggatgagtt gcgaacgggt gagtaacgcg taggtaacct
      181 gcctggtagc gggggataac tattggaaac gatagctaat accgcataag agtagatgtt
      241 gcatgacatt tgcttaaaag gtgcacttgc atcactacca gatggacctg cg
//
LOCUS       DAVBCW010000233          289 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.233, whole genome shotgun sequence.
ACCESSION   DAVBCW010000233 DAVBCW010000000
VERSION     DAVBCW010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.233"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            <1..>289
                     /locus_tag="VQ514_002102"
     CDS             <1..>289
                     /locus_tag="VQ514_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HET2500789.1"
                     /translation="VKEPLTALQLVNTATPVGFGLAYFIAKLLKKNIYTQEEIETLKS
                     AVPMGIVNIVEGVIPIVMNNLVPGLIATGIGGAVGGAVSLTMGADSAVPFGG"
BASE COUNT           74 a           46 c           72 g           97 t
ORIGIN      
        1 tgtgaaagaa ccattgactg ctttgcaatt ggtgaatact gctacaccag ttggatttgg
       61 attggcctat tttatcgcga aattactcaa aaaaaatatc tatactcaag aggaaatcga
      121 aacattgaaa tcggctgttc ctatggggat tgtcaatatt gttgaaggtg taattccgat
      181 tgttatgaat aacttggttc caggtctcat tgcaacaggt atcggtggtg ctgttggtgg
      241 tgctgtttct ttgacaatgg gtgctgattc tgccgtgcca tttggtgga
//
LOCUS       DAVBCW010000234          279 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.234, whole genome shotgun sequence.
ACCESSION   DAVBCW010000234 DAVBCW010000000
VERSION     DAVBCW010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.234"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
BASE COUNT           83 a           53 c           77 g           66 t
ORIGIN      
        1 tttttttgaa tcaatagaaa aatcagcctg aatcatttag gctgatttta agtacgacgg
       61 gcatgtcgtg catctgaggt gtaagtcctc cgtgggcacc tgctaccggt gaacccaata
      121 gcgattccca agcctgacta tcgtgaggta gcggattaaa acggtctggg gatagaccgt
      181 tttaagtctg acgcaggaaa taagaatcgt cagaggaagg gatagcgaaa tcgaggctct
      241 acgaacagga acgtgatagt aaggcgtata cagcggata
//
LOCUS       DAVBCW010000235          277 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.235, whole genome shotgun sequence.
ACCESSION   DAVBCW010000235 DAVBCW010000000
VERSION     DAVBCW010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.235"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            <1..>277
                     /locus_tag="VQ514_002103"
     CDS             <1..>277
                     /locus_tag="VQ514_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2500790.1"
                     /translation="QRIIEAIESALEKVDEYAESYNRLEQLDKEFPDKLKKSILQYAM
                     QGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQG"
BASE COUNT          126 a           37 c           47 g           67 t
ORIGIN      
        1 aacaacgcat aatagaagca atcgaatcag ctttagaaaa agtagatgaa tatgctgaaa
       61 gttataatag actagaacag ctagataaag aatttccaga taaactaaaa aaatctattc
      121 ttcaatatgc tatgcaagga aaattagttg aacaagaccc aaatgatgaa tcagtcgaag
      181 ttttacttga aaaaatacga gcagaaaaac aaaaactctt tgaagaaggc aagattaaaa
      241 agaaagattt ggacatttct attgtttccc aaggaga
//
LOCUS       DAVBCW010000236          270 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.236, whole genome shotgun sequence.
ACCESSION   DAVBCW010000236 DAVBCW010000000
VERSION     DAVBCW010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.236"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            <1..>270
                     /locus_tag="VQ514_002104"
     rRNA            <1..>270
                     /locus_tag="VQ514_002104"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           69 a           74 c           72 g           55 t
ORIGIN      
        1 tgccggtaat aaaccggagg aaggtgggga tgacgtcaaa tcatcatgcc ccttatgacc
       61 tgggctacac acgtgctaca atggctggta caacgagtcg caagccggtg acggcaagct
      121 aatctcttaa agccagtctc agttcggatt gtaggctgca actcgcctac atgaagtcgg
      181 aatcgctagt aatcgcggat cagcacgccg cggtgaatac gttcccgggc cttgtacaca
      241 ccgcccgtca caccacgaga gtttgtaaca
//
LOCUS       DAVBCW010000237          268 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.237, whole genome shotgun sequence.
ACCESSION   DAVBCW010000237 DAVBCW010000000
VERSION     DAVBCW010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.237"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            <1..186
                     /locus_tag="VQ514_002105"
     CDS             <1..186
                     /locus_tag="VQ514_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500791.1"
                     /translation="IFTKQIRHWILSDQVISGKRKLFFREDTPKEILDLYENIKSKLD
                     FAYQEVHSNNGLKKYEK"
BASE COUNT          100 a           33 c           44 g           91 t
ORIGIN      
        1 atttttacaa aacaaattcg tcattggatt cttagtgatc aagttatttc agggaaaaga
       61 aaacttttct tccgggaaga tactccaaaa gagattttag atttgtacga aaacattaaa
      121 tctaaacttg attttgctta ccaagaagtt cattctaata atgggttgaa aaaatatgaa
      181 aaatagaata attgatgttt ttgaagtagt aaatcgcctc cttgtaataa ctgttgaaaa
      241 tcctgatttc gaagacttga gagttaat
//
LOCUS       DAVBCW010000238          267 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.239, whole genome shotgun sequence.
ACCESSION   DAVBCW010000238 DAVBCW010000000
VERSION     DAVBCW010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.239"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
BASE COUNT          108 a           29 c           48 g           82 t
ORIGIN      
        1 cttggagtat ttgtttgtgt gtttactttt atataacaaa ctataaacac aacaaaaata
       61 taaaaaaaga gacaaaaaag aacagaaagt aattaacaac gatttcatat agtgttatac
      121 ttatagcata aagttaatgg aaagaaggga aaaccttatg gtattagatt atttctttga
      181 caaaaacctt gtgttttgct tagaagcgga taatcaagaa taactctttg atcaggtagc
      241 aagcttgttg gaggaacgag aaattgt
//
LOCUS       DAVBCW010000239          267 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.240, whole genome shotgun sequence.
ACCESSION   DAVBCW010000239 DAVBCW010000000
VERSION     DAVBCW010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.240"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            158..>267
                     /locus_tag="VQ514_002106"
     CDS             158..>267
                     /locus_tag="VQ514_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HET2500792.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEQLFDQVATLLEEREIV"
BASE COUNT          108 a           28 c           48 g           83 t
ORIGIN      
        1 cttggagtat ttgtttgtgt gtttattttt atataacaaa ctataaacaa aacaaaaata
       61 taaaaaaaga gacaaaaaag aacagaaagc aattgacaac ggtttcatat agtgttatac
      121 ttatggcata aagttaatgg aaagaaggga aaaacttatg gtattagatt atttctttga
      181 caaaaacctt gtgttttgct tagaagcgga taatcaagaa caactctttg atcaggttgc
      241 aactttattg gaagaacgag aaattgt
//
LOCUS       DAVBCW010000240          264 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.241, whole genome shotgun sequence.
ACCESSION   DAVBCW010000240 DAVBCW010000000
VERSION     DAVBCW010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.241"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(<1..>264)
                     /locus_tag="VQ514_002107"
     CDS             complement(<1..>264)
                     /locus_tag="VQ514_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2500793.1"
                     /translation="HQLAISTSTVIRKLNDFHFEHDFSRLPKIMSWDEYAFTKGKMSF
                     IAQDFDNLNIITVLEGRTQAVIRNHFLRYDRAVRCQVKIITMDM"
BASE COUNT           86 a           51 c           54 g           73 t
ORIGIN      
        1 catatccatc gtaatgattt tcacttgaca acgaacggct ctatcgtagc gaagaaagtg
       61 atttcggatg acagcttgtg ttctgccttc aagaacagtg ataatattaa gattatcaaa
      121 atcttgcgca atgaaactca tctttccctt agtgaaggca tactcatccc aagacataat
      181 ctttggaagc cgagaaaaat catgctcaaa gtgaaagtca ttgagcttgc gaatgacagt
      241 tgaagttgaa atggccagct gatg
//
LOCUS       DAVBCW010000241          251 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.242, whole genome shotgun sequence.
ACCESSION   DAVBCW010000241 DAVBCW010000000
VERSION     DAVBCW010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.242"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            1..251
                     /locus_tag="VQ514_002108"
                     /pseudo
     CDS             1..251
                     /locus_tag="VQ514_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
BASE COUNT           82 a           53 c           51 g           65 t
ORIGIN      
        1 ttacaattga tgccttattt caggcttata aaaaggagtt aggtcgttcc tacacacgtg
       61 atgccttcta tcaactgttg aagcgccatg gttggcgaaa tattacgcca cgtccagaac
      121 atcctaagaa agcagacgct caaaccattg tcgcgtctaa aaataaaatc tcaattcaag
      181 aagacaagaa agcgctttaa aaccagtaga cgttttcata aggttcgctt gatgtaccaa
      241 gatgaggctg g
//
LOCUS       DAVBCW010000242          239 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.243, whole genome shotgun sequence.
ACCESSION   DAVBCW010000242 DAVBCW010000000
VERSION     DAVBCW010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.243"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            16..>239
                     /gene="tnpB"
                     /locus_tag="VQ514_002109"
     CDS             16..>239
                     /gene="tnpB"
                     /locus_tag="VQ514_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HET2500794.1"
                     /translation="MMIVLSDLGQVHLVCGKTDMRQGIDSLAYVVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDGQGFWLLYKRFENG"
BASE COUNT           64 a           40 c           57 g           78 t
ORIGIN      
        1 gaaaggtatt gtactatgat gattgtacta agtgatttag gtcaagttca ccttgtttgt
       61 ggcaagacag atatgaggca gggaatagac tcattagcct atgtagttaa aacccacttt
      121 gaattggatc ctttctccgg tcaagtcttt ctcttttgtg gtggacgtaa agaccgcttt
      181 aaagcccttt actgggatgg tcaaggattt tggctactat ataaacgctt tgagaacgg
//
LOCUS       DAVBCW010000243          230 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.244, whole genome shotgun sequence.
ACCESSION   DAVBCW010000243 DAVBCW010000000
VERSION     DAVBCW010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..230
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.244"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            1..230
                     /locus_tag="VQ514_002110"
                     /pseudo
     CDS             1..230
                     /locus_tag="VQ514_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT           80 a           44 c           47 g           59 t
ORIGIN      
        1 aaaagattct ccactatcat aaccaaaaat ttttgcctga aatgatggtt atggctaaag
       61 gggttaacgt gggaatttca accatttact attggattca tcatggaaaa ttggggtaaa
      121 gcaagcagga tctgctttac cctagaaaag gaaaagtgct taagaaacag gccagcacca
      181 actttaaacc tgctggtcaa tccatcgaac agcggcttga agctatcaat
//
LOCUS       DAVBCW010000244          227 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.245, whole genome shotgun sequence.
ACCESSION   DAVBCW010000244 DAVBCW010000000
VERSION     DAVBCW010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.245"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            complement(47..>227)
                     /locus_tag="VQ514_002111"
     CDS             complement(47..>227)
                     /locus_tag="VQ514_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2500795.1"
                     /translation="LSSPIVQSVIEEKASGSTKQKELLTKTIKEYLIPLPPLPEQSRI
                     VDKIEQFFAHIDALI"
BASE COUNT           68 a           32 c           46 g           81 t
ORIGIN      
        1 ctatcttatg tttgatagaa attccaccgc acgtaaaact attatactaa attagtgcgt
       61 caatatgggc gaaaaattgt tcgattttat caacgattct ggattgttca ggaaggggtg
      121 ggagggggat taaatattct tttatagttt tcgttaataa ttctttttgt tttgtactac
      181 ccgacgcttt ttcttcaata actgactgaa caataggaga ggaaaga
//
LOCUS       DAVBCW010000245          227 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.246, whole genome shotgun sequence.
ACCESSION   DAVBCW010000245 DAVBCW010000000
VERSION     DAVBCW010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.246"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
BASE COUNT           71 a           43 c           32 g           81 t
ORIGIN      
        1 gatttcttct ttagttgatt tcatattact attgtataat gctttttgat ttgagtctag
       61 tattatactc ttcgaaaatc tcttcaaacc gcgtcagctt cgccttgtcg tatagatgtt
      121 actgacttcg tcagttctat ctacaaccta aaaacagtgt tttgagcagt ctgcagctaa
      181 ctttctataa caaaatgaaa taagaacaag acaaatcaat caagaca
//
LOCUS       DAVBCW010000246          212 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN18679
            SAMEA1919805-rid20186813.denovo.247, whole genome shotgun sequence.
ACCESSION   DAVBCW010000246 DAVBCW010000000
VERSION     DAVBCW010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919805
            Sequence Read Archive: ERR298089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980254.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980254.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:57:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,070
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 42
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 55 of 105
            Pseudo Genes (incomplete)         :: 42 of 105
            Pseudo Genes (internal stop)      :: 30 of 105
            Pseudo Genes (multiple problems)  :: 19 of 105
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919805-rid20186813.denovo.247"
                     /strain="MN18679"
                     /db_xref="taxon:1313"
     gene            <1..>212
                     /locus_tag="VQ514_002112"
     CDS             <1..>212
                     /locus_tag="VQ514_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2500796.1"
                     /translation="ADSYRNYFDFWYRLSLDKVKNMLVKNTVTPLWEGYQAPGGWPDK
                     YGKRNNKEDYAPLRELFGPIGKYYGN"
BASE COUNT           71 a           25 c           49 g           67 t
ORIGIN      
        1 gcggacagtt atcgaaatta ttttgatttt tggtatagat tatctttaga taaggttaag
       61 aatatgctag tcaaaaatac agtaactcct ctttgggaag gttatcaagc acctggtgga
      121 tggcctgata agtatggtaa gagaaataat aaagaagact atgccccatt gagagagttg
      181 tttggaccta ttggtaagta ttatggtaac aa
//
